id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001787-lj1nd922	Liu, Ying	Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis	2014-04-02		.txt	text/plain	3105	174	56	title: Enhancing production of ergosterol in Pichia pastoris GS115 by over-expression of 3-hydroxy-3-methylglutaryl CoA reductase from Glycyrrhiza uralensis pastoris strains containing different copy numbers of the GuHMGR gene were obtained and the content of ergosterol was analyzed by HPLC. In this study, we investigated how copy number variation (CNV) of the GuHMGR gene affects the formation of ergosterol. pastoris strains containing different copy numbers of the GuHMGR gene were induced to express the gene; P. pastoris strains was 1.07-2.51 times higher than in the negative control but with increase in the copy number of GuHMGR gene; the content of ergosterol showed an increasing-decreasingincreasing pattern. pastoris strain containing 44 copies of the GuHMGR gene. In this study, the dependence of the content of ergosterol on the copy number of the GuHMGR gene suggests that an increase in the latter could lead to an increase in the production of GA in G.	./cache/cord-001787-lj1nd922.txt	./txt/cord-001787-lj1nd922.txt