id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000483-zgapjjjw	Faux, Cassandra E.	Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection	2011-02-17		.txt	text/plain	1668	83	48	We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. We conducted a preliminary comparison of the relative sensitivity of a cross-section of published HRV-specifi c PCR primer pairs (most of which were fi rst published before HRV-C was reported), independent of most variables described above, by testing a panel of 57 clinical specimen nucleic acid extracts from combined nose and throat swabs from preschool children with colds and infl uenza-like illnesses in Melbourne, Australia. Five primer pairs, including real-time PCR (rtPCR) pair 5, did not amplify the HEVs, a positive feature for HRV-specifi c studies. We next selected 4 frequently published primer pairs (1, 5, 7, and 8) to examine 44 picornavirus-positive specimens (39 HRVs, 3 HEVs, and 2 untypeable picornaviruses) from nonhospitalized children with acute asthma exacerbation (6) .	./cache/cord-000483-zgapjjjw.txt	./txt/cord-000483-zgapjjjw.txt