id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-356207-tpn5cg4n	Beniac, Daniel R	Architecture of the SARS coronavirus prefusion spike	2006-07-16		.txt	text/plain	801	48	51	The emergence in 2003 of a new coronavirus (CoV) responsible for the atypical pneumonia termed severe acute respiratory syndrome (SARS) was a stark reminder that hitherto unknown viruses have the potential to cross species barriers to become new human pathogens. Here we describe the SARS-CoV 'spike' structure determined by single-particle cryo-EM, along with the docked atomic structures of the receptor-binding domain and prefusion core. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/nsmb1123) contains supplementary material, which is available to authorized users. SARS-CoV-enriched fractions were checked by SDS-Page and Western blotting, and rendered non-infectious by irradiation in a gamma cell on dry ice with a 2 Mrad exposure for 90 minutes. Formvar-carbon coated 400-mesh nickel grids were floated on drops of purified SARS-CoV (40μl) for 1 minute. Structure of SARS coronavirus spike receptor-binding domain complexed with receptor Solution structure of the severe acute respiratory syndrome-coronavirus heptad repeat 2 domain in the prefusion state	./cache/cord-356207-tpn5cg4n.txt	./txt/cord-356207-tpn5cg4n.txt