id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309742-fd1qmr87	Slepushkin, Vladimir A.	Infection of Human Airway Epithelia with H1N1, H2N2, and H3N2 Influenza A Virus Strains	2016-12-14		.txt	text/plain	5526	311	48	Therefore, we assayed A/PR/8/34 (H1N1), A/Japan/305/57 (H2N2), and X31 (H3N2) influenza virus strains for binding and infection on fully differentiated primary cultures of airway epithelia isolated from human bronchus, grown on semiporous filters at an air–liquid interface. These data show that influenza viruses with SAα2,3Gal binding specificity, like Japan, productively infect differentiated human airway epithelia from the apical surface. For measurement of the kinetics of influenza virus production from infected airway cells in one infectious cycle, 100 l of the virus preparation was diluted in Dulbecco's PBS (Life Technologies, Inc., Grand Island, NY) and applied to the apical surface of the epithelia (m.o.i. of 0.1). To infect airway epithelia with different influenza virus strains from the basolateral side, the Millicell culture insert was turned over and virus preparations diluted in PBS were applied in a 100-l volume to the bottom of the membrane (m.o.i. ϭ 0.1).	./cache/cord-309742-fd1qmr87.txt	./txt/cord-309742-fd1qmr87.txt