id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303381-xvzhb7ix	TSURUTA, Yuya	The requirement of environmental acidification for Ibaraki virus infection to host cells	2015-08-28		.txt	text/plain	1987	121	49	The effect of environmental acidification on Ibaraki virus (IBAV) infection was tested using endosomal inhibitory chemicals and low pH treatment. Treatment of target cells with endosomal inhibitors significantly decreased the progeny virus production. HmLu-1 (hamster lung) cells were infected with IBAV in the presence of three different endosome inhibitors, bafilomycin A1 (Baf A1, Sigma, St. Louis, MO, U.S.A.), chlorpromazine (CPZ, Abcam, Cambridge, U.K.) and dynasore (Wako, Osaka, Japan). These results indicated that IBAV utilizes clathrin-dependent endosomal pathway for infection and coincided with the previous research on bluetongue virus entry [8] . To confirm the effect of low pH on virus infectivity, purified IBAV was incubated in PBS (−) with several pH (pH=4, 7 or 9) for five min and infected to HmLu-1 at MOI=0.01. The result obtained above implicated that low pH treat-ment removes IBAV outer capsid proteins from the particle and initiates its infection.	./cache/cord-303381-xvzhb7ix.txt	./txt/cord-303381-xvzhb7ix.txt