id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-009800-tajkpgkj	Orellana, Mhica V.	An immunoprobe to measure Rubisco concentrations and maximal photosynthetic rates of individual phytoplankton cells	2003-12-22		.txt	text/plain	5874	306	42	The cross‐reactivity of an immunological probe to the key photosynthetic enzyme Rubisco (ribulose‐1,5‐bisphosphate carboxylase/oxygenase) was characterized as part of a larger effort to determine maximal photosynthetic rates of individual phytoplankton cells. In order to use an immunoprobe to quantitatively measure Rubisco concentration within individual cells in a mixed-species assemblage, it is imperative to determine: first, whether the probe is truly specific to only the large subunit of Rubisco; second, whether the probe binds to the Rubisco large subunit of all phytoplankton species with equal affinity; and third, whether immunologically determined concentrations of Rubisco are well correlated with maximal photosynthetic rates. The results indicate that an affinity-purified anti-Rubisco probe can be used to quantify Rubisco concentrations and maximal photosynthetic potential of individual phytoplankton cells in mixed-species assemblages. When the more quantitative dot blot assays were used with purified Rubisco from different species, a high degree of variability in the binding affinity with the polyclonal antiserum was evident (Fig. 1) .	./cache/cord-009800-tajkpgkj.txt	./txt/cord-009800-tajkpgkj.txt