id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-294559-u0r7oh9z	Bian, Hongfen	A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay	2019-01-18		.txt	text/plain	5654	346	63	title: A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay For rapid detection of PEDV, a new immunochromatographic assay (ICA) based on monoclonal antibodies (mAbs) was developed in this study. To compare its accuracy to other traditional detection methods, 27 swine stool samples from south of China were investigated with the new developed ICA, commercial strip and RT-PCR. Relying on signals emitted from gold nanoparticles labeled mAb (AuNPs-mAb), a new ICA was developed for sensitive, specific and on-site detection of PEDV in swine stool in China. They were capture and detection mAb, the size of gold nanoparticles, the type of sample pad, the type of conjugate pad, the type of Nitrocellulose membrane, the type of absorbent pad, the amount of tween-20 addition and the spray volume of AuNPs-mAb. The optimization methods are shown in the supplemental materials.	./cache/cord-294559-u0r7oh9z.txt	./txt/cord-294559-u0r7oh9z.txt