id sid tid token lemma pos cord-329061-1xut73dq 1 1 key key NN cord-329061-1xut73dq 1 2 : : : cord-329061-1xut73dq 2 1 cord-329061 cord-329061 NNP cord-329061-1xut73dq 2 2 - - HYPH cord-329061-1xut73dq 2 3 1xut73dq 1xut73dq CD cord-329061-1xut73dq 2 4 authors author NNS cord-329061-1xut73dq 2 5 : : : cord-329061-1xut73dq 2 6 Bhatt Bhatt NNP cord-329061-1xut73dq 2 7 , , , cord-329061-1xut73dq 2 8 Pravin Pravin NNP cord-329061-1xut73dq 2 9 N. N. NNP cord-329061-1xut73dq 2 10 ; ; : cord-329061-1xut73dq 2 11 Percy Percy NNP cord-329061-1xut73dq 2 12 , , , cord-329061-1xut73dq 2 13 Dean Dean NNP cord-329061-1xut73dq 2 14 H. H. NNP cord-329061-1xut73dq 2 15 ; ; : cord-329061-1xut73dq 2 16 Jonas Jonas NNP cord-329061-1xut73dq 2 17 , , , cord-329061-1xut73dq 2 18 Albert Albert NNP cord-329061-1xut73dq 2 19 M. M. NNP cord-329061-1xut73dq 2 20 title title NN cord-329061-1xut73dq 2 21 : : : cord-329061-1xut73dq 2 22 Characterization characterization NN cord-329061-1xut73dq 2 23 of of IN cord-329061-1xut73dq 2 24 the the DT cord-329061-1xut73dq 2 25 Virus Virus NNP cord-329061-1xut73dq 2 26 of of IN cord-329061-1xut73dq 2 27 Sialodacryoadenitis Sialodacryoadenitis NNP cord-329061-1xut73dq 2 28 of of IN cord-329061-1xut73dq 2 29 Rats Rats NNPS cord-329061-1xut73dq 2 30 : : : cord-329061-1xut73dq 3 1 A a DT cord-329061-1xut73dq 3 2 Member Member NNP cord-329061-1xut73dq 3 3 of of IN cord-329061-1xut73dq 3 4 the the DT cord-329061-1xut73dq 3 5 Coronavirus Coronavirus NNP cord-329061-1xut73dq 3 6 Group Group NNP cord-329061-1xut73dq 3 7 date date NN cord-329061-1xut73dq 3 8 : : : cord-329061-1xut73dq 3 9 1972 1972 CD cord-329061-1xut73dq 3 10 - - SYM cord-329061-1xut73dq 3 11 08 08 CD cord-329061-1xut73dq 3 12 - - SYM cord-329061-1xut73dq 3 13 17 17 CD cord-329061-1xut73dq 3 14 journal journal NN cord-329061-1xut73dq 3 15 : : : cord-329061-1xut73dq 4 1 J J NNP cord-329061-1xut73dq 4 2 Infect infect VBP cord-329061-1xut73dq 4 3 Dis Dis NNP cord-329061-1xut73dq 4 4 DOI doi NN cord-329061-1xut73dq 5 1 : : : cord-329061-1xut73dq 5 2 10.1093 10.1093 CD cord-329061-1xut73dq 5 3 / / SYM cord-329061-1xut73dq 5 4 infdis/126.2.123 infdis/126.2.123 NN cord-329061-1xut73dq 5 5 sha sha NNP cord-329061-1xut73dq 5 6 : : : cord-329061-1xut73dq 5 7 e3b7b10c6eef820f3a93188926cae2f6296f34b4 e3b7b10c6eef820f3a93188926cae2f6296f34b4 NNP cord-329061-1xut73dq 5 8 doc_id doc_id CD cord-329061-1xut73dq 5 9 : : : cord-329061-1xut73dq 5 10 329061 329061 CD cord-329061-1xut73dq 6 1 cord_uid cord_uid NN cord-329061-1xut73dq 6 2 : : : cord-329061-1xut73dq 7 1 1xut73dq 1xut73dq CD cord-329061-1xut73dq 8 1 The the DT cord-329061-1xut73dq 8 2 virus virus NN cord-329061-1xut73dq 8 3 that that WDT cord-329061-1xut73dq 8 4 causes cause VBZ cord-329061-1xut73dq 8 5 sialodacryoadenitis sialodacryoadenitis NN cord-329061-1xut73dq 8 6 in in IN cord-329061-1xut73dq 8 7 rats rat NNS cord-329061-1xut73dq 8 8 has have VBZ cord-329061-1xut73dq 8 9 been be VBN cord-329061-1xut73dq 8 10 isolated isolate VBN cord-329061-1xut73dq 8 11 in in IN cord-329061-1xut73dq 8 12 mice mouse NNS cord-329061-1xut73dq 8 13 and and CC cord-329061-1xut73dq 8 14 in in IN cord-329061-1xut73dq 8 15 primary primary JJ cord-329061-1xut73dq 8 16 cultures culture NNS cord-329061-1xut73dq 8 17 of of IN cord-329061-1xut73dq 8 18 rat rat NN cord-329061-1xut73dq 8 19 - - HYPH cord-329061-1xut73dq 8 20 kidney kidney NN cord-329061-1xut73dq 8 21 cells cell NNS cord-329061-1xut73dq 8 22 and and CC cord-329061-1xut73dq 8 23 has have VBZ cord-329061-1xut73dq 8 24 been be VBN cord-329061-1xut73dq 8 25 characterized characterize VBN cord-329061-1xut73dq 8 26 as as IN cord-329061-1xut73dq 8 27 a a DT cord-329061-1xut73dq 8 28 heat heat NN cord-329061-1xut73dq 8 29 - - HYPH cord-329061-1xut73dq 8 30 labile labile JJ cord-329061-1xut73dq 8 31 RNA RNA NNP cord-329061-1xut73dq 8 32 virus virus NN cord-329061-1xut73dq 8 33 that that WDT cord-329061-1xut73dq 8 34 is be VBZ cord-329061-1xut73dq 8 35 sensitive sensitive JJ cord-329061-1xut73dq 8 36 to to IN cord-329061-1xut73dq 8 37 lipid lipid NN cord-329061-1xut73dq 8 38 solvents solvent NNS cord-329061-1xut73dq 8 39 and and CC cord-329061-1xut73dq 8 40 is be VBZ cord-329061-1xut73dq 8 41 relatively relatively RB cord-329061-1xut73dq 8 42 stable stable JJ cord-329061-1xut73dq 8 43 at at IN cord-329061-1xut73dq 8 44 pH pH NNP cord-329061-1xut73dq 8 45 3.0 3.0 CD cord-329061-1xut73dq 8 46 . . . cord-329061-1xut73dq 9 1 This this DT cord-329061-1xut73dq 9 2 virus virus NN cord-329061-1xut73dq 9 3 is be VBZ cord-329061-1xut73dq 9 4 antigenically antigenically RB cord-329061-1xut73dq 9 5 related relate VBN cord-329061-1xut73dq 9 6 to to IN cord-329061-1xut73dq 9 7 the the DT cord-329061-1xut73dq 9 8 virus virus NN cord-329061-1xut73dq 9 9 of of IN cord-329061-1xut73dq 9 10 hepatitis hepatitis NN cord-329061-1xut73dq 9 11 in in IN cord-329061-1xut73dq 9 12 mice mouse NNS cord-329061-1xut73dq 9 13 and and CC cord-329061-1xut73dq 9 14 to to IN cord-329061-1xut73dq 9 15 coronavirus coronavirus NN cord-329061-1xut73dq 9 16 of of IN cord-329061-1xut73dq 9 17 rats rat NNS cord-329061-1xut73dq 9 18 . . . cord-329061-1xut73dq 10 1 The the DT cord-329061-1xut73dq 10 2 range range NN cord-329061-1xut73dq 10 3 of of IN cord-329061-1xut73dq 10 4 hosts host NNS cord-329061-1xut73dq 10 5 of of IN cord-329061-1xut73dq 10 6 this this DT cord-329061-1xut73dq 10 7 agent agent NN cord-329061-1xut73dq 10 8 appears appear VBZ cord-329061-1xut73dq 10 9 to to TO cord-329061-1xut73dq 10 10 be be VB cord-329061-1xut73dq 10 11 narrow narrow JJ cord-329061-1xut73dq 10 12 . . . cord-329061-1xut73dq 11 1 On on IN cord-329061-1xut73dq 11 2 the the DT cord-329061-1xut73dq 11 3 basis basis NN cord-329061-1xut73dq 11 4 of of IN cord-329061-1xut73dq 11 5 available available JJ cord-329061-1xut73dq 11 6 biologic biologic JJ cord-329061-1xut73dq 11 7 characteristics characteristic NNS cord-329061-1xut73dq 11 8 , , , cord-329061-1xut73dq 11 9 it -PRON- PRP cord-329061-1xut73dq 11 10 has have VBZ cord-329061-1xut73dq 11 11 been be VBN cord-329061-1xut73dq 11 12 placed place VBN cord-329061-1xut73dq 11 13 in in IN cord-329061-1xut73dq 11 14 the the DT cord-329061-1xut73dq 11 15 coronavirus coronavirus NN cord-329061-1xut73dq 11 16 group group NN cord-329061-1xut73dq 11 17 . . . cord-329061-1xut73dq 12 1 cated cat VBN cord-329061-1xut73dq 12 2 that that IN cord-329061-1xut73dq 12 3 neither neither DT cord-329061-1xut73dq 12 4 source source NN cord-329061-1xut73dq 12 5 had have VBD cord-329061-1xut73dq 12 6 detectable detectable JJ cord-329061-1xut73dq 12 7 serum serum NN cord-329061-1xut73dq 12 8 antibodies antibody NNS cord-329061-1xut73dq 12 9 to to IN cord-329061-1xut73dq 12 10 SDA SDA NNP cord-329061-1xut73dq 12 11 virus virus NN cord-329061-1xut73dq 12 12 and and CC cord-329061-1xut73dq 12 13 that that IN cord-329061-1xut73dq 12 14 both both DT cord-329061-1xut73dq 12 15 were be VBD cord-329061-1xut73dq 12 16 susceptible susceptible JJ cord-329061-1xut73dq 12 17 to to IN cord-329061-1xut73dq 12 18 infection infection NN cord-329061-1xut73dq 12 19 with with IN cord-329061-1xut73dq 12 20 this this DT cord-329061-1xut73dq 12 21 agent agent NN cord-329061-1xut73dq 12 22 . . . cord-329061-1xut73dq 13 1 Rats rat NNS cord-329061-1xut73dq 13 2 were be VBD cord-329061-1xut73dq 13 3 inoculated inoculate VBN cord-329061-1xut73dq 13 4 intranasally intranasally RB cord-329061-1xut73dq 13 5 with with IN cord-329061-1xut73dq 13 6 0.1 0.1 CD cord-329061-1xut73dq 13 7 ml ml NNS cord-329061-1xut73dq 13 8 of of IN cord-329061-1xut73dq 13 9 virus virus NN cord-329061-1xut73dq 13 10 - - HYPH cord-329061-1xut73dq 13 11 infected infect VBN cord-329061-1xut73dq 13 12 salivary salivary NN cord-329061-1xut73dq 13 13 - - HYPH cord-329061-1xut73dq 13 14 gland gland NN cord-329061-1xut73dq 13 15 suspension suspension NN cord-329061-1xut73dq 13 16 , , , cord-329061-1xut73dq 13 17 observed observe VBD cord-329061-1xut73dq 13 18 daily daily RB cord-329061-1xut73dq 13 19 for for IN cord-329061-1xut73dq 13 20 evidence evidence NN cord-329061-1xut73dq 13 21 of of IN cord-329061-1xut73dq 13 22 overt overt JJ cord-329061-1xut73dq 13 23 illness illness NN cord-329061-1xut73dq 13 24 , , , cord-329061-1xut73dq 13 25 and and CC cord-329061-1xut73dq 13 26 sacrificed sacrifice VBD cord-329061-1xut73dq 13 27 at at IN cord-329061-1xut73dq 13 28 various various JJ cord-329061-1xut73dq 13 29 intervals interval NNS cord-329061-1xut73dq 13 30 . . . cord-329061-1xut73dq 14 1 All all DT cord-329061-1xut73dq 14 2 rats rat NNS cord-329061-1xut73dq 14 3 were be VBD cord-329061-1xut73dq 14 4 maintained maintain VBN cord-329061-1xut73dq 14 5 in in IN cord-329061-1xut73dq 14 6 rigid rigid JJ cord-329061-1xut73dq 14 7 plastic plastic JJ cord-329061-1xut73dq 14 8 isolators isolator NNS cord-329061-1xut73dq 14 9 with with IN cord-329061-1xut73dq 14 10 high high JJ cord-329061-1xut73dq 14 11 - - HYPH cord-329061-1xut73dq 14 12 efficiency efficiency NN cord-329061-1xut73dq 14 13 air air NN cord-329061-1xut73dq 14 14 filters filter NNS cord-329061-1xut73dq 14 15 . . . cord-329061-1xut73dq 15 1 Tissue tissue NN cord-329061-1xut73dq 15 2 culture culture NN cord-329061-1xut73dq 15 3 . . . cord-329061-1xut73dq 16 1 Three three CD cord-329061-1xut73dq 16 2 cell cell NN cord-329061-1xut73dq 16 3 lines line NNS cord-329061-1xut73dq 16 4 , , , cord-329061-1xut73dq 16 5 baby baby NN cord-329061-1xut73dq 16 6 - - HYPH cord-329061-1xut73dq 16 7 hamster hamster NN cord-329061-1xut73dq 16 8 kidney kidney NN cord-329061-1xut73dq 16 9 ( ( -LRB- cord-329061-1xut73dq 16 10 BHK-21 BHK-21 NNP cord-329061-1xut73dq 16 11 ) ) -RRB- cord-329061-1xut73dq 16 12 , , , cord-329061-1xut73dq 16 13 VERa VERa NNS cord-329061-1xut73dq 16 14 , , , cord-329061-1xut73dq 16 15 and and CC cord-329061-1xut73dq 16 16 Hep-2 Hep-2 NNP cord-329061-1xut73dq 16 17 , , , cord-329061-1xut73dq 16 18 and and CC cord-329061-1xut73dq 16 19 primary primary JJ cord-329061-1xut73dq 16 20 monolayer monolayer NN cord-329061-1xut73dq 16 21 cultures culture NNS cord-329061-1xut73dq 16 22 of of IN cord-329061-1xut73dq 16 23 rat rat NN cord-329061-1xut73dq 16 24 embryo embryo NN cord-329061-1xut73dq 16 25 , , , cord-329061-1xut73dq 16 26 rabbit rabbit NN cord-329061-1xut73dq 16 27 kidney kidney NN cord-329061-1xut73dq 16 28 , , , cord-329061-1xut73dq 16 29 rhesus rhesus NN cord-329061-1xut73dq 16 30 - - HYPH cord-329061-1xut73dq 16 31 monkey monkey NN cord-329061-1xut73dq 16 32 kidney kidney NN cord-329061-1xut73dq 16 33 , , , cord-329061-1xut73dq 16 34 guinea guinea NN cord-329061-1xut73dq 16 35 - - HYPH cord-329061-1xut73dq 16 36 pig pig NN cord-329061-1xut73dq 16 37 - - HYPH cord-329061-1xut73dq 16 38 embryo embryo NN cord-329061-1xut73dq 16 39 skin skin NN cord-329061-1xut73dq 16 40 , , , cord-329061-1xut73dq 16 41 muscle muscle NN cord-329061-1xut73dq 16 42 , , , cord-329061-1xut73dq 16 43 and and CC cord-329061-1xut73dq 16 44 kidney kidney NN cord-329061-1xut73dq 16 45 were be VBD cord-329061-1xut73dq 16 46 used use VBN cord-329061-1xut73dq 16 47 as as IN cord-329061-1xut73dq 16 48 previously previously RB cord-329061-1xut73dq 16 49 described describe VBN cord-329061-1xut73dq 16 50 [ [ -LRB- cord-329061-1xut73dq 16 51 l l NN cord-329061-1xut73dq 16 52 ] ] -RRB- cord-329061-1xut73dq 16 53 . . . cord-329061-1xut73dq 17 1 Monolayers monolayer NNS cord-329061-1xut73dq 17 2 obtained obtain VBD cord-329061-1xut73dq 17 3 from from IN cord-329061-1xut73dq 17 4 explant explant JJ cord-329061-1xut73dq 17 5 cultures culture NNS cord-329061-1xut73dq 17 6 of of IN cord-329061-1xut73dq 17 7 submaxillary submaxillary NN cord-329061-1xut73dq 17 8 , , , cord-329061-1xut73dq 17 9 parotid parotid NN cord-329061-1xut73dq 17 10 , , , cord-329061-1xut73dq 17 11 Harderian Harderian NNP cord-329061-1xut73dq 17 12 , , , cord-329061-1xut73dq 17 13 and and CC cord-329061-1xut73dq 17 14 exorbital exorbital JJ cord-329061-1xut73dq 17 15 glands gland NNS cord-329061-1xut73dq 17 16 of of IN cord-329061-1xut73dq 17 17 germfree germfree JJ cord-329061-1xut73dq 17 18 rats rat NNS cord-329061-1xut73dq 17 19 , , , cord-329061-1xut73dq 17 20 monolayers monolayer NNS cord-329061-1xut73dq 17 21 of of IN cord-329061-1xut73dq 17 22 trypsin trypsin NN cord-329061-1xut73dq 17 23 - - HYPH cord-329061-1xut73dq 17 24 dispersed disperse VBN cord-329061-1xut73dq 17 25 brain brain NN cord-329061-1xut73dq 17 26 cells cell NNS cord-329061-1xut73dq 17 27 of of IN cord-329061-1xut73dq 17 28 infant infant NN cord-329061-1xut73dq 17 29 mice mouse NNS cord-329061-1xut73dq 17 30 , , , cord-329061-1xut73dq 17 31 and and CC cord-329061-1xut73dq 17 32 a a DT cord-329061-1xut73dq 17 33 line line NN cord-329061-1xut73dq 17 34 of of IN cord-329061-1xut73dq 17 35 polyoma polyoma NN cord-329061-1xut73dq 17 36 - - HYPH cord-329061-1xut73dq 17 37 transformed transform VBN cord-329061-1xut73dq 17 38 mouse mouse NN cord-329061-1xut73dq 17 39 cells cell NNS cord-329061-1xut73dq 17 40 ( ( -LRB- cord-329061-1xut73dq 17 41 Py Py NNP cord-329061-1xut73dq 17 42 - - HYPH cord-329061-1xut73dq 17 43 AL AL NNP cord-329061-1xut73dq 17 44 / / SYM cord-329061-1xut73dq 17 45 N N NNP cord-329061-1xut73dq 17 46 ) ) -RRB- cord-329061-1xut73dq 17 47 [ [ -LRB- cord-329061-1xut73dq 17 48 6J 6j CD cord-329061-1xut73dq 17 49 were be VBD cord-329061-1xut73dq 17 50 also also RB cord-329061-1xut73dq 17 51 tested test VBN cord-329061-1xut73dq 17 52 . . . cord-329061-1xut73dq 18 1 At at IN cord-329061-1xut73dq 18 2 a a DT cord-329061-1xut73dq 18 3 later later JJ cord-329061-1xut73dq 18 4 stage stage NN cord-329061-1xut73dq 18 5 in in IN cord-329061-1xut73dq 18 6 the the DT cord-329061-1xut73dq 18 7 study study NN cord-329061-1xut73dq 18 8 , , , cord-329061-1xut73dq 18 9 primary primary JJ cord-329061-1xut73dq 18 10 rat rat NN cord-329061-1xut73dq 18 11 - - HYPH cord-329061-1xut73dq 18 12 kidney kidney NN cord-329061-1xut73dq 18 13 ( ( -LRB- cord-329061-1xut73dq 18 14 PRK PRK NNP cord-329061-1xut73dq 18 15 ) ) -RRB- cord-329061-1xut73dq 18 16 cultures culture NNS cord-329061-1xut73dq 18 17 prepared prepare VBN cord-329061-1xut73dq 18 18 from from IN cord-329061-1xut73dq 18 19 kidneys kidney NNS cord-329061-1xut73dq 18 20 of of IN cord-329061-1xut73dq 18 21 weanling weanle VBG cord-329061-1xut73dq 18 22 Charles Charles NNP cord-329061-1xut73dq 18 23 River River NNP cord-329061-1xut73dq 18 24 CD CD NNP cord-329061-1xut73dq 18 25 germfree germfree NN cord-329061-1xut73dq 18 26 or or CC cord-329061-1xut73dq 18 27 conventional conventional JJ cord-329061-1xut73dq 18 28 inbred inbred JJ cord-329061-1xut73dq 18 29 dark dark JJ cord-329061-1xut73dq 18 30 Agouti Agouti NNP cord-329061-1xut73dq 18 31 fDA fDA '' cord-329061-1xut73dq 18 32 ) ) -RRB- cord-329061-1xut73dq 18 33 rats rat NNS cord-329061-1xut73dq 18 34 were be VBD cord-329061-1xut73dq 18 35 used use VBN cord-329061-1xut73dq 18 36 . . . cord-329061-1xut73dq 19 1 Inoculated inoculate VBN cord-329061-1xut73dq 19 2 tubes tube NNS cord-329061-1xut73dq 19 3 were be VBD cord-329061-1xut73dq 19 4 kept keep VBN cord-329061-1xut73dq 19 5 in in IN cord-329061-1xut73dq 19 6 a a DT cord-329061-1xut73dq 19 7 roller roller NN cord-329061-1xut73dq 19 8 drum drum NN cord-329061-1xut73dq 19 9 at at IN cord-329061-1xut73dq 19 10 37 37 CD cord-329061-1xut73dq 19 11 C c NN cord-329061-1xut73dq 19 12 and and CC cord-329061-1xut73dq 19 13 observed observe VBD cord-329061-1xut73dq 19 14 for for IN cord-329061-1xut73dq 19 15 cytopathic cytopathic JJ cord-329061-1xut73dq 19 16 effect effect NN cord-329061-1xut73dq 19 17 ( ( -LRB- cord-329061-1xut73dq 19 18 CPE CPE NNP cord-329061-1xut73dq 19 19 ) ) -RRB- cord-329061-1xut73dq 19 20 at at IN cord-329061-1xut73dq 19 21 intervals interval NNS cord-329061-1xut73dq 19 22 of of IN cord-329061-1xut73dq 19 23 two two CD cord-329061-1xut73dq 19 24 to to TO cord-329061-1xut73dq 19 25 four four CD cord-329061-1xut73dq 19 26 days day NNS cord-329061-1xut73dq 19 27 for for IN cord-329061-1xut73dq 19 28 at at RB cord-329061-1xut73dq 19 29 least least RBS cord-329061-1xut73dq 19 30 21 21 CD cord-329061-1xut73dq 19 31 days day NNS cord-329061-1xut73dq 19 32 . . . cord-329061-1xut73dq 20 1 The the DT cord-329061-1xut73dq 20 2 fluid fluid JJ cord-329061-1xut73dq 20 3 medium medium NN cord-329061-1xut73dq 20 4 was be VBD cord-329061-1xut73dq 20 5 changed change VBN cord-329061-1xut73dq 20 6 when when WRB cord-329061-1xut73dq 20 7 necessary necessary JJ cord-329061-1xut73dq 20 8 . . . cord-329061-1xut73dq 21 1 In in IN cord-329061-1xut73dq 21 2 the the DT cord-329061-1xut73dq 21 3 absence absence NN cord-329061-1xut73dq 21 4 of of IN cord-329061-1xut73dq 21 5 CPE CPE NNP cord-329061-1xut73dq 21 6 , , , cord-329061-1xut73dq 21 7 a a DT cord-329061-1xut73dq 21 8 blind blind JJ cord-329061-1xut73dq 21 9 passage passage NN cord-329061-1xut73dq 21 10 was be VBD cord-329061-1xut73dq 21 11 made make VBN cord-329061-1xut73dq 21 12 between between IN cord-329061-1xut73dq 21 13 the the DT cord-329061-1xut73dq 21 14 eighth eighth JJ cord-329061-1xut73dq 21 15 and and CC cord-329061-1xut73dq 21 16 16th 16th JJ cord-329061-1xut73dq 21 17 day day NN cord-329061-1xut73dq 21 18 after after IN cord-329061-1xut73dq 21 19 inoculation inoculation NN cord-329061-1xut73dq 21 20 . . . cord-329061-1xut73dq 22 1 Cultures culture NNS cord-329061-1xut73dq 22 2 for for IN cord-329061-1xut73dq 22 3 passage passage NN cord-329061-1xut73dq 22 4 were be VBD cord-329061-1xut73dq 22 5 observed observe VBN cord-329061-1xut73dq 22 6 for for IN cord-329061-1xut73dq 22 7 one one CD cord-329061-1xut73dq 22 8 to to TO cord-329061-1xut73dq 22 9 two two CD cord-329061-1xut73dq 22 10 weeks week NNS cord-329061-1xut73dq 22 11 for for IN cord-329061-1xut73dq 22 12 development development NN cord-329061-1xut73dq 22 13 of of IN cord-329061-1xut73dq 22 14 CPE CPE NNP cord-329061-1xut73dq 22 15 , , , cord-329061-1xut73dq 22 16 and and CC cord-329061-1xut73dq 22 17 , , , cord-329061-1xut73dq 22 18 in in IN cord-329061-1xut73dq 22 19 the the DT cord-329061-1xut73dq 22 20 absence absence NN cord-329061-1xut73dq 22 21 of of IN cord-329061-1xut73dq 22 22 CPE CPE NNP cord-329061-1xut73dq 22 23 , , , cord-329061-1xut73dq 22 24 VERa VERa NNS cord-329061-1xut73dq 22 25 , , , cord-329061-1xut73dq 22 26 BHK-21 BHK-21 NNP cord-329061-1xut73dq 22 27 , , , cord-329061-1xut73dq 22 28 and and CC cord-329061-1xut73dq 22 29 PMK PMK NNP cord-329061-1xut73dq 22 30 cultures culture NNS cord-329061-1xut73dq 22 31 were be VBD cord-329061-1xut73dq 22 32 challenged challenge VBN cord-329061-1xut73dq 22 33 on on IN cord-329061-1xut73dq 22 34 the the DT cord-329061-1xut73dq 22 35 12th 12th JJ cord-329061-1xut73dq 22 36 day day NN cord-329061-1xut73dq 22 37 after after IN cord-329061-1xut73dq 22 38 inoculation inoculation NN cord-329061-1xut73dq 22 39 with with IN cord-329061-1xut73dq 22 40 Chandipura Chandipura NNP cord-329061-1xut73dq 22 41 [ [ -LRB- cord-329061-1xut73dq 22 42 8J 8J NNP cord-329061-1xut73dq 22 43 virus virus NN cord-329061-1xut73dq 22 44 , , , cord-329061-1xut73dq 22 45 an an DT cord-329061-1xut73dq 22 46 arbovirus arbovirus NN cord-329061-1xut73dq 22 47 of of IN cord-329061-1xut73dq 22 48 the the DT cord-329061-1xut73dq 22 49 vesicular vesicular JJ cord-329061-1xut73dq 22 50 stomatitis stomatitis NN cord-329061-1xut73dq 22 51 viral viral JJ cord-329061-1xut73dq 22 52 group group NN cord-329061-1xut73dq 22 53 , , , cord-329061-1xut73dq 22 54 for for IN cord-329061-1xut73dq 22 55 determination determination NN cord-329061-1xut73dq 22 56 of of IN cord-329061-1xut73dq 22 57 interference interference NN cord-329061-1xut73dq 22 58 . . . cord-329061-1xut73dq 23 1 In in IN cord-329061-1xut73dq 23 2 addition addition NN cord-329061-1xut73dq 23 3 , , , cord-329061-1xut73dq 23 4 the the DT cord-329061-1xut73dq 23 5 fluid fluid NN cord-329061-1xut73dq 23 6 from from IN cord-329061-1xut73dq 23 7 each each DT cord-329061-1xut73dq 23 8 of of IN cord-329061-1xut73dq 23 9 the the DT cord-329061-1xut73dq 23 10 second second JJ cord-329061-1xut73dq 23 11 - - HYPH cord-329061-1xut73dq 23 12 passage passage NN cord-329061-1xut73dq 23 13 cultures culture NNS cord-329061-1xut73dq 23 14 was be VBD cord-329061-1xut73dq 23 15 inoculated inoculate VBN cord-329061-1xut73dq 23 16 ic ic NNP cord-329061-1xut73dq 23 17 into into IN cord-329061-1xut73dq 23 18 infant infant JJ cord-329061-1xut73dq 23 19 mice mouse NNS cord-329061-1xut73dq 23 20 , , , cord-329061-1xut73dq 23 21 and and CC cord-329061-1xut73dq 23 22 the the DT cord-329061-1xut73dq 23 23 mice mouse NNS cord-329061-1xut73dq 23 24 were be VBD cord-329061-1xut73dq 23 25 observed observe VBN cord-329061-1xut73dq 23 26 for for IN cord-329061-1xut73dq 23 27 21 21 CD cord-329061-1xut73dq 23 28 days day NNS cord-329061-1xut73dq 23 29 . . . cord-329061-1xut73dq 24 1 In in IN cord-329061-1xut73dq 24 2 some some DT cord-329061-1xut73dq 24 3 instances instance NNS cord-329061-1xut73dq 24 4 tissueculture tissueculture VBP cord-329061-1xut73dq 24 5 fluid fluid NN cord-329061-1xut73dq 24 6 from from IN cord-329061-1xut73dq 24 7 inoculated inoculate VBN cord-329061-1xut73dq 24 8 tubes tube NNS cord-329061-1xut73dq 24 9 was be VBD cord-329061-1xut73dq 24 10 passaged passage VBN cord-329061-1xut73dq 24 11 into into IN cord-329061-1xut73dq 24 12 infant infant NN cord-329061-1xut73dq 24 13 mice mouse NNS cord-329061-1xut73dq 24 14 without without IN cord-329061-1xut73dq 24 15 further further JJ cord-329061-1xut73dq 24 16 passages passage NNS cord-329061-1xut73dq 24 17 in in IN cord-329061-1xut73dq 24 18 tissue tissue NN cord-329061-1xut73dq 24 19 culture culture NN cord-329061-1xut73dq 24 20 . . . cord-329061-1xut73dq 25 1 Monolayer Monolayer NNP cord-329061-1xut73dq 25 2 cultures culture NNS cord-329061-1xut73dq 25 3 of of IN cord-329061-1xut73dq 25 4 PRK PRK NNP cord-329061-1xut73dq 25 5 , , , cord-329061-1xut73dq 25 6 infant infant NN cord-329061-1xut73dq 25 7 - - HYPH cord-329061-1xut73dq 25 8 mouse mouse NN cord-329061-1xut73dq 25 9 brain brain NN cord-329061-1xut73dq 25 10 , , , cord-329061-1xut73dq 25 11 and and CC cord-329061-1xut73dq 25 12 Py Py NNP cord-329061-1xut73dq 25 13 - - HYPH cord-329061-1xut73dq 25 14 AL AL NNP cord-329061-1xut73dq 25 15 / / SYM cord-329061-1xut73dq 25 16 N n CD cord-329061-1xut73dq 25 17 cells cell NNS cord-329061-1xut73dq 25 18 were be VBD cord-329061-1xut73dq 25 19 also also RB cord-329061-1xut73dq 25 20 examined examine VBN cord-329061-1xut73dq 25 21 by by IN cord-329061-1xut73dq 25 22 indirect indirect JJ cord-329061-1xut73dq 25 23 immunofluorescence immunofluorescence NN cord-329061-1xut73dq 25 24 for for IN cord-329061-1xut73dq 25 25 the the DT cord-329061-1xut73dq 25 26 presence presence NN cord-329061-1xut73dq 25 27 of of IN cord-329061-1xut73dq 25 28 viral viral JJ cord-329061-1xut73dq 25 29 antigen antigen NN cord-329061-1xut73dq 25 30 [ [ -LRB- cord-329061-1xut73dq 25 31 5 5 CD cord-329061-1xut73dq 25 32 ] ] -RRB- cord-329061-1xut73dq 25 33 . . . cord-329061-1xut73dq 26 1 Characterization characterization NN cord-329061-1xut73dq 26 2 of of IN cord-329061-1xut73dq 26 3 the the DT cord-329061-1xut73dq 26 4 virus virus NN cord-329061-1xut73dq 26 5 . . . cord-329061-1xut73dq 27 1 For for IN cord-329061-1xut73dq 27 2 determination determination NN cord-329061-1xut73dq 27 3 of of IN cord-329061-1xut73dq 27 4 the the DT cord-329061-1xut73dq 27 5 effect effect NN cord-329061-1xut73dq 27 6 of of IN cord-329061-1xut73dq 27 7 5-bromodeoxyuridine 5-bromodeoxyuridine CD cord-329061-1xut73dq 27 8 ( ( -LRB- cord-329061-1xut73dq 27 9 5-BUDR 5-budr CD cord-329061-1xut73dq 27 10 ) ) -RRB- cord-329061-1xut73dq 27 11 , , , cord-329061-1xut73dq 27 12 lipid lipid NN cord-329061-1xut73dq 27 13 solvent solvent NN cord-329061-1xut73dq 27 14 , , , cord-329061-1xut73dq 27 15 low low JJ cord-329061-1xut73dq 27 16 pH ph NN cord-329061-1xut73dq 27 17 , , , cord-329061-1xut73dq 27 18 and and CC cord-329061-1xut73dq 27 19 various various JJ cord-329061-1xut73dq 27 20 temperatures temperature NNS cord-329061-1xut73dq 27 21 , , , cord-329061-1xut73dq 27 22 methods method NNS cord-329061-1xut73dq 27 23 described describe VBN cord-329061-1xut73dq 27 24 by by IN cord-329061-1xut73dq 27 25 Bhatt Bhatt NNP cord-329061-1xut73dq 27 26 et et NNP cord-329061-1xut73dq 27 27 al al NNP cord-329061-1xut73dq 27 28 . . . cord-329061-1xut73dq 28 1 [ [ -LRB- cord-329061-1xut73dq 28 2 9J 9j CD cord-329061-1xut73dq 28 3 were be VBD cord-329061-1xut73dq 28 4 used use VBN cord-329061-1xut73dq 28 5 . . . cord-329061-1xut73dq 29 1 The the DT cord-329061-1xut73dq 29 2 hemagglutination hemagglutination NN cord-329061-1xut73dq 29 3 method method NN cord-329061-1xut73dq 29 4 will will MD cord-329061-1xut73dq 29 5 be be VB cord-329061-1xut73dq 29 6 Bhatt Bhatt NNP cord-329061-1xut73dq 29 7 , , , cord-329061-1xut73dq 29 8 Percy Percy NNP cord-329061-1xut73dq 29 9 , , , cord-329061-1xut73dq 29 10 and and CC cord-329061-1xut73dq 29 11 Jonas Jonas NNP cord-329061-1xut73dq 29 12 described describe VBD cord-329061-1xut73dq 29 13 under under IN cord-329061-1xut73dq 29 14 Results result NNS cord-329061-1xut73dq 29 15 . . . cord-329061-1xut73dq 30 1 Staining stain VBG cord-329061-1xut73dq 30 2 with with IN cord-329061-1xut73dq 30 3 acridine acridine NNP cord-329061-1xut73dq 30 4 orange orange NNP cord-329061-1xut73dq 30 5 was be VBD cord-329061-1xut73dq 30 6 done do VBN cord-329061-1xut73dq 30 7 according accord VBG cord-329061-1xut73dq 30 8 to to IN cord-329061-1xut73dq 30 9 the the DT cord-329061-1xut73dq 30 10 method method NN cord-329061-1xut73dq 30 11 of of IN cord-329061-1xut73dq 30 12 Hsiung Hsiung NNP cord-329061-1xut73dq 30 13 [ [ -LRB- cord-329061-1xut73dq 30 14 10 10 CD cord-329061-1xut73dq 30 15 ] ] -RRB- cord-329061-1xut73dq 30 16 . . . cord-329061-1xut73dq 31 1 Preparation preparation NN cord-329061-1xut73dq 31 2 of of IN cord-329061-1xut73dq 31 3 immune immune JJ cord-329061-1xut73dq 31 4 sera sera NNP cord-329061-1xut73dq 31 5 . . . cord-329061-1xut73dq 32 1 Hyperimmune Hyperimmune NNP cord-329061-1xut73dq 32 2 sera sera NNP cord-329061-1xut73dq 32 3 were be VBD cord-329061-1xut73dq 32 4 prepared prepare VBN cord-329061-1xut73dq 32 5 in in IN cord-329061-1xut73dq 32 6 rats rat NNS cord-329061-1xut73dq 32 7 and and CC cord-329061-1xut73dq 32 8 mice mouse NNS cord-329061-1xut73dq 32 9 by by IN cord-329061-1xut73dq 32 10 repeated repeat VBN cord-329061-1xut73dq 32 11 inoculation inoculation NN cord-329061-1xut73dq 32 12 of of IN cord-329061-1xut73dq 32 13 a a DT cord-329061-1xut73dq 32 14 suspension suspension NN cord-329061-1xut73dq 32 15 of of IN cord-329061-1xut73dq 32 16 salivary salivary JJ cord-329061-1xut73dq 32 17 glands gland NNS cord-329061-1xut73dq 32 18 from from IN cord-329061-1xut73dq 32 19 infected infected JJ cord-329061-1xut73dq 32 20 rats rat NNS cord-329061-1xut73dq 32 21 and and CC cord-329061-1xut73dq 32 22 of of IN cord-329061-1xut73dq 32 23 brains brain NNS cord-329061-1xut73dq 32 24 from from IN cord-329061-1xut73dq 32 25 infected infected JJ cord-329061-1xut73dq 32 26 mice mouse NNS cord-329061-1xut73dq 32 27 , , , cord-329061-1xut73dq 32 28 respectively respectively RB cord-329061-1xut73dq 32 29 . . . cord-329061-1xut73dq 33 1 Complement complement NN cord-329061-1xut73dq 33 2 - - HYPH cord-329061-1xut73dq 33 3 fixation fixation NN cord-329061-1xut73dq 33 4 test test NN cord-329061-1xut73dq 33 5 . . . cord-329061-1xut73dq 34 1 Complement complement NN cord-329061-1xut73dq 34 2 - - HYPH cord-329061-1xut73dq 34 3 fixing fix VBG cord-329061-1xut73dq 34 4 antigen antigen NN cord-329061-1xut73dq 34 5 was be VBD cord-329061-1xut73dq 34 6 prepared prepare VBN cord-329061-1xut73dq 34 7 by by IN cord-329061-1xut73dq 34 8 sucrose sucrose NN cord-329061-1xut73dq 34 9 - - HYPH cord-329061-1xut73dq 34 10 acetone acetone NN cord-329061-1xut73dq 34 11 extraction extraction NN cord-329061-1xut73dq 34 12 from from IN cord-329061-1xut73dq 34 13 infected infected JJ cord-329061-1xut73dq 34 14 brains brain NNS cord-329061-1xut73dq 34 15 of of IN cord-329061-1xut73dq 34 16 two two CD cord-329061-1xut73dq 34 17 - - HYPH cord-329061-1xut73dq 34 18 to to TO cord-329061-1xut73dq 34 19 four four CD cord-329061-1xut73dq 34 20 - - HYPH cord-329061-1xut73dq 34 21 day day NN cord-329061-1xut73dq 34 22 - - HYPH cord-329061-1xut73dq 34 23 old old JJ cord-329061-1xut73dq 34 24 mice mouse NNS cord-329061-1xut73dq 34 25 . . . cord-329061-1xut73dq 35 1 Polyvalent Polyvalent NNP cord-329061-1xut73dq 35 2 mouse mouse NN cord-329061-1xut73dq 35 3 - - HYPH cord-329061-1xut73dq 35 4 hepatitis hepatitis NN cord-329061-1xut73dq 35 5 CF cf NN cord-329061-1xut73dq 35 6 antigen antigen NN cord-329061-1xut73dq 35 7 prepared prepare VBN cord-329061-1xut73dq 35 8 in in IN cord-329061-1xut73dq 35 9 tissue tissue NN cord-329061-1xut73dq 35 10 culture culture NN cord-329061-1xut73dq 35 11 was be VBD cord-329061-1xut73dq 35 12 obtained obtain VBN cord-329061-1xut73dq 35 13 from from IN cord-329061-1xut73dq 35 14 Microbiological Microbiological NNP cord-329061-1xut73dq 35 15 Associates Associates NNPS cord-329061-1xut73dq 35 16 . . . cord-329061-1xut73dq 36 1 The the DT cord-329061-1xut73dq 36 2 CF CF NNP cord-329061-1xut73dq 36 3 test test NN cord-329061-1xut73dq 36 4 was be VBD cord-329061-1xut73dq 36 5 performed perform VBN cord-329061-1xut73dq 36 6 by by IN cord-329061-1xut73dq 36 7 the the DT cord-329061-1xut73dq 36 8 micromethod micromethod NNP cord-329061-1xut73dq 36 9 [ [ -LRB- cord-329061-1xut73dq 36 10 l l NNP cord-329061-1xut73dq 36 11 l l NNP cord-329061-1xut73dq 36 12 ] ] -RRB- cord-329061-1xut73dq 36 13 using use VBG cord-329061-1xut73dq 36 14 two two CD cord-329061-1xut73dq 36 15 units unit NNS cord-329061-1xut73dq 36 16 of of IN cord-329061-1xut73dq 36 17 complement complement NN cord-329061-1xut73dq 36 18 and and CC cord-329061-1xut73dq 36 19 four four CD cord-329061-1xut73dq 36 20 to to TO cord-329061-1xut73dq 36 21 eight eight CD cord-329061-1xut73dq 36 22 units unit NNS cord-329061-1xut73dq 36 23 of of IN cord-329061-1xut73dq 36 24 antigen antigen NN cord-329061-1xut73dq 36 25 . . . cord-329061-1xut73dq 37 1 Serum serum NN cord-329061-1xut73dq 37 2 of of IN cord-329061-1xut73dq 37 3 mice mouse NNS cord-329061-1xut73dq 37 4 immune immune JJ cord-329061-1xut73dq 37 5 to to IN cord-329061-1xut73dq 37 6 SDA SDA NNP cord-329061-1xut73dq 37 7 strain strain VB cord-329061-1xut73dq 37 8 681 681 CD cord-329061-1xut73dq 37 9 was be VBD cord-329061-1xut73dq 37 10 tested test VBN cord-329061-1xut73dq 37 11 against against IN cord-329061-1xut73dq 37 12 118 118 CD cord-329061-1xut73dq 37 13 viral viral JJ cord-329061-1xut73dq 37 14 antigens antigen NNS cord-329061-1xut73dq 37 15 by by IN cord-329061-1xut73dq 37 16 the the DT cord-329061-1xut73dq 37 17 CF CF NNP cord-329061-1xut73dq 37 18 test test NN cord-329061-1xut73dq 37 19 . . . cord-329061-1xut73dq 38 1 Neutralization neutralization NN cord-329061-1xut73dq 38 2 test test NN cord-329061-1xut73dq 38 3 . . . cord-329061-1xut73dq 39 1 The the DT cord-329061-1xut73dq 39 2 neutralization neutralization NN cord-329061-1xut73dq 39 3 ( ( -LRB- cord-329061-1xut73dq 39 4 N n NN cord-329061-1xut73dq 39 5 ) ) -RRB- cord-329061-1xut73dq 39 6 test test NN cord-329061-1xut73dq 39 7 with with IN cord-329061-1xut73dq 39 8 sera sera NNP cord-329061-1xut73dq 39 9 immune immune NN cord-329061-1xut73dq 39 10 to to IN cord-329061-1xut73dq 39 11 murine murine JJ cord-329061-1xut73dq 39 12 viruses virus NNS cord-329061-1xut73dq 39 13 was be VBD cord-329061-1xut73dq 39 14 performed perform VBN cord-329061-1xut73dq 39 15 in in IN cord-329061-1xut73dq 39 16 infant infant NN cord-329061-1xut73dq 39 17 mice mouse NNS cord-329061-1xut73dq 39 18 , , , cord-329061-1xut73dq 39 19 and and CC cord-329061-1xut73dq 39 20 these these DT cord-329061-1xut73dq 39 21 animals animal NNS cord-329061-1xut73dq 39 22 were be VBD cord-329061-1xut73dq 39 23 observed observe VBN cord-329061-1xut73dq 39 24 for for IN cord-329061-1xut73dq 39 25 14 14 CD cord-329061-1xut73dq 39 26 days day NNS cord-329061-1xut73dq 39 27 after after IN cord-329061-1xut73dq 39 28 inoculation inoculation NN cord-329061-1xut73dq 39 29 . . . cord-329061-1xut73dq 40 1 PRK PRK NNP cord-329061-1xut73dq 40 2 cultures culture NNS cord-329061-1xut73dq 40 3 were be VBD cord-329061-1xut73dq 40 4 used use VBN cord-329061-1xut73dq 40 5 for for IN cord-329061-1xut73dq 40 6 cross cross JJ cord-329061-1xut73dq 40 7 - - JJ cord-329061-1xut73dq 40 8 N n JJ cord-329061-1xut73dq 40 9 tests test NNS cord-329061-1xut73dq 40 10 , , , cord-329061-1xut73dq 40 11 using use VBG cord-329061-1xut73dq 40 12 SDA SDA NNP cord-329061-1xut73dq 40 13 strain strain VB cord-329061-1xut73dq 40 14 681 681 CD cord-329061-1xut73dq 40 15 and and CC cord-329061-1xut73dq 40 16 Parker Parker NNP cord-329061-1xut73dq 40 17 's 's POS cord-329061-1xut73dq 40 18 RCV RCV NNP cord-329061-1xut73dq 40 19 . . . cord-329061-1xut73dq 41 1 Sera Sera NNP cord-329061-1xut73dq 41 2 were be VBD cord-329061-1xut73dq 41 3 inactivated inactivate VBN cord-329061-1xut73dq 41 4 at at IN cord-329061-1xut73dq 41 5 56 56 CD cord-329061-1xut73dq 41 6 C c NN cord-329061-1xut73dq 41 7 for for IN cord-329061-1xut73dq 41 8 30 30 CD cord-329061-1xut73dq 41 9 min min NN cord-329061-1xut73dq 41 10 . . . cord-329061-1xut73dq 42 1 Cultures culture NNS cord-329061-1xut73dq 42 2 were be VBD cord-329061-1xut73dq 42 3 examined examine VBN cord-329061-1xut73dq 42 4 on on IN cord-329061-1xut73dq 42 5 the the DT cord-329061-1xut73dq 42 6 third third JJ cord-329061-1xut73dq 42 7 and and CC cord-329061-1xut73dq 42 8 fifth fifth JJ cord-329061-1xut73dq 42 9 days day NNS cord-329061-1xut73dq 42 10 after after IN cord-329061-1xut73dq 42 11 inoculation inoculation NN cord-329061-1xut73dq 42 12 . . . cord-329061-1xut73dq 43 1 The the DT cord-329061-1xut73dq 43 2 titers titer NNS cord-329061-1xut73dq 43 3 of of IN cord-329061-1xut73dq 43 4 antibody antibody NN cord-329061-1xut73dq 43 5 and and CC cord-329061-1xut73dq 43 6 virus virus NN cord-329061-1xut73dq 43 7 were be VBD cord-329061-1xut73dq 43 8 calculated calculate VBN cord-329061-1xut73dq 43 9 by by IN cord-329061-1xut73dq 43 10 the the DT cord-329061-1xut73dq 43 11 method method NN cord-329061-1xut73dq 43 12 of of IN cord-329061-1xut73dq 43 13 Reed Reed NNP cord-329061-1xut73dq 43 14 and and CC cord-329061-1xut73dq 43 15 Muench Muench NNP cord-329061-1xut73dq 43 16 [ [ -LRB- cord-329061-1xut73dq 43 17 12J. 12j. CD cord-329061-1xut73dq 43 18 Fluorescent fluorescent JJ cord-329061-1xut73dq 43 19 - - HYPH cord-329061-1xut73dq 43 20 antibody antibody NN cord-329061-1xut73dq 43 21 method method NN cord-329061-1xut73dq 43 22 . . . cord-329061-1xut73dq 44 1 Pieces piece NNS cord-329061-1xut73dq 44 2 of of IN cord-329061-1xut73dq 44 3 mouse mouse NN cord-329061-1xut73dq 44 4 brain brain NN cord-329061-1xut73dq 44 5 2 2 CD cord-329061-1xut73dq 44 6 - - SYM cord-329061-1xut73dq 44 7 4 4 CD cord-329061-1xut73dq 44 8 mm mm NN cord-329061-1xut73dq 44 9 thick thick JJ cord-329061-1xut73dq 44 10 were be VBD cord-329061-1xut73dq 44 11 quick quick RB cord-329061-1xut73dq 44 12 - - HYPH cord-329061-1xut73dq 44 13 frozen frozen JJ cord-329061-1xut73dq 44 14 in in IN cord-329061-1xut73dq 44 15 a a DT cord-329061-1xut73dq 44 16 dry dry JJ cord-329061-1xut73dq 44 17 ice ice NN cord-329061-1xut73dq 44 18 - - HYPH cord-329061-1xut73dq 44 19 alcohol alcohol NN cord-329061-1xut73dq 44 20 bath bath NN cord-329061-1xut73dq 44 21 and and CC cord-329061-1xut73dq 44 22 stored store VBN cord-329061-1xut73dq 44 23 at at IN cord-329061-1xut73dq 44 24 -83 -83 NNP cord-329061-1xut73dq 44 25 C. C. NNP cord-329061-1xut73dq 44 26 Sections Sections NNP cord-329061-1xut73dq 44 27 6 6 CD cord-329061-1xut73dq 44 28 - - HYPH cord-329061-1xut73dq 44 29 8f!m 8f!m NN cord-329061-1xut73dq 44 30 thick thick NN cord-329061-1xut73dq 44 31 were be VBD cord-329061-1xut73dq 44 32 cut cut VBN cord-329061-1xut73dq 44 33 in in IN cord-329061-1xut73dq 44 34 a a DT cord-329061-1xut73dq 44 35 cryostat cryostat NN cord-329061-1xut73dq 44 36 , , , cord-329061-1xut73dq 44 37 two two CD cord-329061-1xut73dq 44 38 sections section NNS cord-329061-1xut73dq 44 39 were be VBD cord-329061-1xut73dq 44 40 mounted mount VBN cord-329061-1xut73dq 44 41 per per IN cord-329061-1xut73dq 44 42 slide slide NN cord-329061-1xut73dq 44 43 , , , cord-329061-1xut73dq 44 44 and and CC cord-329061-1xut73dq 44 45 then then RB cord-329061-1xut73dq 44 46 the the DT cord-329061-1xut73dq 44 47 slides slide NNS cord-329061-1xut73dq 44 48 were be VBD cord-329061-1xut73dq 44 49 fixed fix VBN cord-329061-1xut73dq 44 50 in in IN cord-329061-1xut73dq 44 51 acetone acetone NN cord-329061-1xut73dq 44 52 at at IN cord-329061-1xut73dq 44 53 25 25 CD cord-329061-1xut73dq 44 54 C c NN cord-329061-1xut73dq 44 55 for for IN cord-329061-1xut73dq 44 56 15 15 CD cord-329061-1xut73dq 44 57 - - SYM cord-329061-1xut73dq 44 58 20 20 CD cord-329061-1xut73dq 44 59 min min NN cord-329061-1xut73dq 44 60 and and CC cord-329061-1xut73dq 44 61 dried dry VBN cord-329061-1xut73dq 44 62 at at IN cord-329061-1xut73dq 44 63 37 37 CD cord-329061-1xut73dq 44 64 C c NN cord-329061-1xut73dq 44 65 for for IN cord-329061-1xut73dq 44 66 15 15 CD cord-329061-1xut73dq 44 67 min min NN cord-329061-1xut73dq 44 68 . . . cord-329061-1xut73dq 45 1 Sections section NNS cord-329061-1xut73dq 45 2 were be VBD cord-329061-1xut73dq 45 3 stained stain VBN cord-329061-1xut73dq 45 4 immediately immediately RB cord-329061-1xut73dq 45 5 or or CC cord-329061-1xut73dq 45 6 stored store VBN cord-329061-1xut73dq 45 7 at at IN cord-329061-1xut73dq 45 8 -25 -25 NN cord-329061-1xut73dq 45 9 C C NNP cord-329061-1xut73dq 45 10 for for IN cord-329061-1xut73dq 45 11 1 1 CD cord-329061-1xut73dq 45 12 - - SYM cord-329061-1xut73dq 45 13 30 30 CD cord-329061-1xut73dq 45 14 days day NNS cord-329061-1xut73dq 45 15 before before IN cord-329061-1xut73dq 45 16 use use NN cord-329061-1xut73dq 45 17 . . . cord-329061-1xut73dq 46 1 Tissue tissue NN cord-329061-1xut73dq 46 2 - - HYPH cord-329061-1xut73dq 46 3 culture culture NN cord-329061-1xut73dq 46 4 cover cover NN cord-329061-1xut73dq 46 5 slips slip NNS cord-329061-1xut73dq 46 6 were be VBD cord-329061-1xut73dq 46 7 similarly similarly RB cord-329061-1xut73dq 46 8 prepared prepared JJ cord-329061-1xut73dq 46 9 but but CC cord-329061-1xut73dq 46 10 at at IN cord-329061-1xut73dq 46 11 times time NNS cord-329061-1xut73dq 46 12 were be VBD cord-329061-1xut73dq 46 13 kept keep VBN cord-329061-1xut73dq 46 14 in in IN cord-329061-1xut73dq 46 15 chilled chilled JJ cord-329061-1xut73dq 46 16 acetone acetone NN cord-329061-1xut73dq 46 17 at at IN cord-329061-1xut73dq 46 18 -25 -25 NN cord-329061-1xut73dq 46 19 C C NNP cord-329061-1xut73dq 46 20 for for IN cord-329061-1xut73dq 46 21 18 18 CD cord-329061-1xut73dq 46 22 hr hr NN cord-329061-1xut73dq 46 23 . . . cord-329061-1xut73dq 47 1 The the DT cord-329061-1xut73dq 47 2 section section NN cord-329061-1xut73dq 47 3 and and CC cord-329061-1xut73dq 47 4 cover cover NN cord-329061-1xut73dq 47 5 - - HYPH cord-329061-1xut73dq 47 6 slip slip NN cord-329061-1xut73dq 47 7 preparations preparation NNS cord-329061-1xut73dq 47 8 were be VBD cord-329061-1xut73dq 47 9 reacted react VBN cord-329061-1xut73dq 47 10 with with IN cord-329061-1xut73dq 47 11 sera sera NNP cord-329061-1xut73dq 47 12 immune immune NN cord-329061-1xut73dq 47 13 to to IN cord-329061-1xut73dq 47 14 virus virus NN cord-329061-1xut73dq 47 15 for for IN cord-329061-1xut73dq 47 16 20 20 CD cord-329061-1xut73dq 47 17 min min NN cord-329061-1xut73dq 47 18 at at IN cord-329061-1xut73dq 47 19 25 25 CD cord-329061-1xut73dq 47 20 C c NN cord-329061-1xut73dq 47 21 and and CC cord-329061-1xut73dq 47 22 then then RB cord-329061-1xut73dq 47 23 exposed expose VBN cord-329061-1xut73dq 47 24 to to IN cord-329061-1xut73dq 47 25 mouse mouse NN cord-329061-1xut73dq 47 26 or or CC cord-329061-1xut73dq 47 27 rat rat NN cord-329061-1xut73dq 47 28 antiglobulin antiglobulin NN cord-329061-1xut73dq 47 29 conjugate conjugate NNP cord-329061-1xut73dq 47 30 for for IN cord-329061-1xut73dq 47 31 20 20 CD cord-329061-1xut73dq 47 32 min min NN cord-329061-1xut73dq 47 33 . . . cord-329061-1xut73dq 48 1 Phosphate phosphate NN cord-329061-1xut73dq 48 2 - - HYPH cord-329061-1xut73dq 48 3 buffered buffer VBN cord-329061-1xut73dq 48 4 saline saline NN cord-329061-1xut73dq 48 5 ( ( -LRB- cord-329061-1xut73dq 48 6 PBS PBS NNP cord-329061-1xut73dq 48 7 ) ) -RRB- cord-329061-1xut73dq 48 8 was be VBD cord-329061-1xut73dq 48 9 used use VBN cord-329061-1xut73dq 48 10 for for IN cord-329061-1xut73dq 48 11 washing washing NN cord-329061-1xut73dq 48 12 . . . cord-329061-1xut73dq 49 1 Preparations preparation NNS cord-329061-1xut73dq 49 2 were be VBD cord-329061-1xut73dq 49 3 examined examine VBN cord-329061-1xut73dq 49 4 with with IN cord-329061-1xut73dq 49 5 a a DT cord-329061-1xut73dq 49 6 Carl Carl NNP cord-329061-1xut73dq 49 7 Zeiss Zeiss NNP cord-329061-1xut73dq 49 8 microscope microscope NN cord-329061-1xut73dq 49 9 fitted fit VBN cord-329061-1xut73dq 49 10 with with IN cord-329061-1xut73dq 49 11 an an DT cord-329061-1xut73dq 49 12 HBO HBO NNP cord-329061-1xut73dq 49 13 200 200 CD cord-329061-1xut73dq 49 14 W/4 W/4 NNP cord-329061-1xut73dq 49 15 supermercury supermercury NNP cord-329061-1xut73dq 49 16 lamp lamp NN cord-329061-1xut73dq 49 17 , , , cord-329061-1xut73dq 49 18 a a DT cord-329061-1xut73dq 49 19 UG-5 UG-5 NNP cord-329061-1xut73dq 49 20 exciter exciter NN cord-329061-1xut73dq 49 21 filter filter NN cord-329061-1xut73dq 49 22 , , , cord-329061-1xut73dq 49 23 and and CC cord-329061-1xut73dq 49 24 a a DT cord-329061-1xut73dq 49 25 47/65 47/65 CD cord-329061-1xut73dq 49 26 barrier barrier NN cord-329061-1xut73dq 49 27 filter filter NN cord-329061-1xut73dq 49 28 . . . cord-329061-1xut73dq 50 1 Histopathology Histopathology NNP cord-329061-1xut73dq 50 2 . . . cord-329061-1xut73dq 51 1 Histologic Histologic NNP cord-329061-1xut73dq 51 2 examination examination NN cord-329061-1xut73dq 51 3 of of IN cord-329061-1xut73dq 51 4 tissue tissue NN cord-329061-1xut73dq 51 5 from from IN cord-329061-1xut73dq 51 6 inoculated inoculate VBN cord-329061-1xut73dq 51 7 rats rat NNS cord-329061-1xut73dq 51 8 included include VBD cord-329061-1xut73dq 51 9 Harderian Harderian NNP cord-329061-1xut73dq 51 10 , , , cord-329061-1xut73dq 51 11 exorbital exorbital JJ cord-329061-1xut73dq 51 12 , , , cord-329061-1xut73dq 51 13 parotid parotid NN cord-329061-1xut73dq 51 14 , , , cord-329061-1xut73dq 51 15 and and CC cord-329061-1xut73dq 51 16 submaxillary submaxillary JJ cord-329061-1xut73dq 51 17 glands gland NNS cord-329061-1xut73dq 51 18 . . . cord-329061-1xut73dq 52 1 In in IN cord-329061-1xut73dq 52 2 suckling suckle VBG cord-329061-1xut73dq 52 3 mice mouse NNS cord-329061-1xut73dq 52 4 , , , cord-329061-1xut73dq 52 5 coronal coronal JJ cord-329061-1xut73dq 52 6 sections section NNS cord-329061-1xut73dq 52 7 of of IN cord-329061-1xut73dq 52 8 brain brain NN cord-329061-1xut73dq 52 9 and and CC cord-329061-1xut73dq 52 10 serial serial JJ cord-329061-1xut73dq 52 11 transverse transverse JJ cord-329061-1xut73dq 52 12 sections section NNS cord-329061-1xut73dq 52 13 of of IN cord-329061-1xut73dq 52 14 the the DT cord-329061-1xut73dq 52 15 thoracic thoracic JJ cord-329061-1xut73dq 52 16 and and CC cord-329061-1xut73dq 52 17 abdominal abdominal JJ cord-329061-1xut73dq 52 18 regions region NNS cord-329061-1xut73dq 52 19 were be VBD cord-329061-1xut73dq 52 20 examined examine VBN cord-329061-1xut73dq 52 21 . . . cord-329061-1xut73dq 53 1 Attempts attempt NNS cord-329061-1xut73dq 53 2 to to TO cord-329061-1xut73dq 53 3 induce induce VB cord-329061-1xut73dq 53 4 disease disease NN cord-329061-1xut73dq 53 5 in in IN cord-329061-1xut73dq 53 6 weanling weanle VBG cord-329061-1xut73dq 53 7 mice mouse NNS cord-329061-1xut73dq 53 8 . . . cord-329061-1xut73dq 54 1 Sixty sixty CD cord-329061-1xut73dq 54 2 female female JJ cord-329061-1xut73dq 54 3 mice mouse NNS cord-329061-1xut73dq 54 4 , , , cord-329061-1xut73dq 54 5 three three CD cord-329061-1xut73dq 54 6 - - HYPH cord-329061-1xut73dq 54 7 to to TO cord-329061-1xut73dq 54 8 four four CD cord-329061-1xut73dq 54 9 - - HYPH cord-329061-1xut73dq 54 10 weeks week NNS cord-329061-1xut73dq 54 11 old old JJ cord-329061-1xut73dq 54 12 , , , cord-329061-1xut73dq 54 13 were be VBD cord-329061-1xut73dq 54 14 given give VBN cord-329061-1xut73dq 54 15 2.5 2.5 CD cord-329061-1xut73dq 54 16 mg mg NN cord-329061-1xut73dq 54 17 of of IN cord-329061-1xut73dq 54 18 cortisone cortisone NN cord-329061-1xut73dq 55 1 i -PRON- PRP cord-329061-1xut73dq 55 2 m be VBP cord-329061-1xut73dq 55 3 twice twice PDT cord-329061-1xut73dq 55 4 a a DT cord-329061-1xut73dq 55 5 week week NN cord-329061-1xut73dq 56 1 beginning begin VBG cord-329061-1xut73dq 56 2 a a DT cord-329061-1xut73dq 56 3 week week NN cord-329061-1xut73dq 56 4 before before IN cord-329061-1xut73dq 56 5 inoculation inoculation NN cord-329061-1xut73dq 56 6 and and CC cord-329061-1xut73dq 56 7 continuing continue VBG cord-329061-1xut73dq 56 8 until until IN cord-329061-1xut73dq 56 9 the the DT cord-329061-1xut73dq 56 10 end end NN cord-329061-1xut73dq 56 11 of of IN cord-329061-1xut73dq 56 12 the the DT cord-329061-1xut73dq 56 13 experiment experiment NN cord-329061-1xut73dq 56 14 . . . cord-329061-1xut73dq 57 1 Twenty twenty CD cord-329061-1xut73dq 57 2 mice mouse NNS cord-329061-1xut73dq 57 3 each each DT cord-329061-1xut73dq 57 4 were be VBD cord-329061-1xut73dq 57 5 inoculated inoculate VBN cord-329061-1xut73dq 57 6 ic ic NNP cord-329061-1xut73dq 57 7 and and CC cord-329061-1xut73dq 57 8 ip ip NN cord-329061-1xut73dq 57 9 with with IN cord-329061-1xut73dq 57 10 0.03 0.03 CD cord-329061-1xut73dq 57 11 ml ml NNS cord-329061-1xut73dq 57 12 and and CC cord-329061-1xut73dq 57 13 0.1 0.1 CD cord-329061-1xut73dq 57 14 ml ml NNS cord-329061-1xut73dq 57 15 of of IN cord-329061-1xut73dq 57 16 viral viral JJ cord-329061-1xut73dq 57 17 suspension suspension NN cord-329061-1xut73dq 57 18 containing contain VBG cord-329061-1xut73dq 57 19 2 2 CD cord-329061-1xut73dq 57 20 X x SYM cord-329061-1xut73dq 57 21 1()3·9 1()3·9 CD cord-329061-1xut73dq 57 22 and and CC cord-329061-1xut73dq 57 23 6.3 6.3 CD cord-329061-1xut73dq 57 24 X x NN cord-329061-1xut73dq 57 25 10 10 CD cord-329061-1xut73dq 57 26 3 3 CD cord-329061-1xut73dq 57 27 . . . cord-329061-1xut73dq 58 1 9 9 CD cord-329061-1xut73dq 58 2 infant infant NN cord-329061-1xut73dq 58 3 mouse mouse NN cord-329061-1xut73dq 58 4 LD50 LD50 NNP cord-329061-1xut73dq 58 5 ( ( -LRB- cord-329061-1xut73dq 58 6 IMLD50 imld50 NN cord-329061-1xut73dq 58 7 ) ) -RRB- cord-329061-1xut73dq 58 8 , , , cord-329061-1xut73dq 58 9 respectively respectively RB cord-329061-1xut73dq 58 10 . . . cord-329061-1xut73dq 59 1 Twenty twenty CD cord-329061-1xut73dq 59 2 control control NN cord-329061-1xut73dq 59 3 mice mouse NNS cord-329061-1xut73dq 59 4 were be VBD cord-329061-1xut73dq 59 5 inoculated inoculate VBN cord-329061-1xut73dq 59 6 with with IN cord-329061-1xut73dq 59 7 diluent diluent NN cord-329061-1xut73dq 59 8 , , , cord-329061-1xut73dq 59 9 10 10 CD cord-329061-1xut73dq 59 10 by by IN cord-329061-1xut73dq 59 11 the the DT cord-329061-1xut73dq 59 12 ic ic NNP cord-329061-1xut73dq 59 13 route route NN cord-329061-1xut73dq 59 14 and and CC cord-329061-1xut73dq 59 15 10 10 CD cord-329061-1xut73dq 59 16 by by IN cord-329061-1xut73dq 59 17 the the DT cord-329061-1xut73dq 59 18 ip ip NN cord-329061-1xut73dq 59 19 route route NN cord-329061-1xut73dq 59 20 . . . cord-329061-1xut73dq 60 1 Another another DT cord-329061-1xut73dq 60 2 group group NN cord-329061-1xut73dq 60 3 of of IN cord-329061-1xut73dq 60 4 controls control NNS cord-329061-1xut73dq 60 5 was be VBD cord-329061-1xut73dq 60 6 neither neither CC cord-329061-1xut73dq 60 7 inoculated inoculate VBN cord-329061-1xut73dq 60 8 nor nor CC cord-329061-1xut73dq 60 9 given give VBN cord-329061-1xut73dq 60 10 cortisone cortisone NN cord-329061-1xut73dq 60 11 . . . cord-329061-1xut73dq 61 1 Six six CD cord-329061-1xut73dq 61 2 mice mouse NNS cord-329061-1xut73dq 61 3 from from IN cord-329061-1xut73dq 61 4 each each DT cord-329061-1xut73dq 61 5 group group NN cord-329061-1xut73dq 61 6 were be VBD cord-329061-1xut73dq 61 7 killed kill VBN cord-329061-1xut73dq 61 8 for for IN cord-329061-1xut73dq 61 9 histologic histologic JJ cord-329061-1xut73dq 61 10 studies study NNS cord-329061-1xut73dq 61 11 on on IN cord-329061-1xut73dq 61 12 the the DT cord-329061-1xut73dq 61 13 seventh seventh JJ cord-329061-1xut73dq 61 14 day day NN cord-329061-1xut73dq 61 15 after after IN cord-329061-1xut73dq 61 16 inoculation inoculation NN cord-329061-1xut73dq 61 17 and and CC cord-329061-1xut73dq 61 18 two two CD cord-329061-1xut73dq 61 19 were be VBD cord-329061-1xut73dq 61 20 killed kill VBN cord-329061-1xut73dq 61 21 on on IN cord-329061-1xut73dq 61 22 the the DT cord-329061-1xut73dq 61 23 14th 14th JJ cord-329061-1xut73dq 61 24 day day NN cord-329061-1xut73dq 61 25 after after IN cord-329061-1xut73dq 61 26 inoculation inoculation NN cord-329061-1xut73dq 61 27 . . . cord-329061-1xut73dq 62 1 Remaining remain VBG cord-329061-1xut73dq 62 2 mice mouse NNS cord-329061-1xut73dq 62 3 were be VBD cord-329061-1xut73dq 62 4 observed observe VBN cord-329061-1xut73dq 62 5 until until IN cord-329061-1xut73dq 62 6 the the DT cord-329061-1xut73dq 62 7 21st 21st JJ cord-329061-1xut73dq 62 8 day day NN cord-329061-1xut73dq 62 9 after after IN cord-329061-1xut73dq 62 10 inoculation inoculation NN cord-329061-1xut73dq 62 11 , , , cord-329061-1xut73dq 62 12 when when WRB cord-329061-1xut73dq 62 13 the the DT cord-329061-1xut73dq 62 14 experiment experiment NN cord-329061-1xut73dq 62 15 was be VBD cord-329061-1xut73dq 62 16 terminated terminate VBN cord-329061-1xut73dq 62 17 . . . cord-329061-1xut73dq 63 1 A a DT cord-329061-1xut73dq 63 2 complete complete JJ cord-329061-1xut73dq 63 3 necropsy necropsy JJ cord-329061-1xut73dq 63 4 was be VBD cord-329061-1xut73dq 63 5 done do VBN cord-329061-1xut73dq 63 6 on on IN cord-329061-1xut73dq 63 7 each each DT cord-329061-1xut73dq 63 8 mouse mouse NN cord-329061-1xut73dq 63 9 . . . cord-329061-1xut73dq 64 1 Induction induction NN cord-329061-1xut73dq 64 2 of of IN cord-329061-1xut73dq 64 3 sialodacryoadenitis sialodacryoadenitis NN cord-329061-1xut73dq 64 4 in in IN cord-329061-1xut73dq 64 5 susceptible susceptible JJ cord-329061-1xut73dq 64 6 rats rat NNS cord-329061-1xut73dq 64 7 by by IN cord-329061-1xut73dq 64 8 mouse mouse NN cord-329061-1xut73dq 64 9 - - HYPH cord-329061-1xut73dq 64 10 brain brain NN cord-329061-1xut73dq 64 11 - - HYPH cord-329061-1xut73dq 64 12 adapted adapt VBN cord-329061-1xut73dq 64 13 virus virus NN cord-329061-1xut73dq 64 14 . . . cord-329061-1xut73dq 65 1 Eleven eleven CD cord-329061-1xut73dq 65 2 rats rat NNS cord-329061-1xut73dq 65 3 , , , cord-329061-1xut73dq 65 4 weighing weigh VBG cord-329061-1xut73dq 65 5 250 250 CD cord-329061-1xut73dq 65 6 g g NN cord-329061-1xut73dq 65 7 and and CC cord-329061-1xut73dq 65 8 from from IN cord-329061-1xut73dq 65 9 a a DT cord-329061-1xut73dq 65 10 colony colony NN cord-329061-1xut73dq 65 11 known know VBN cord-329061-1xut73dq 65 12 to to TO cord-329061-1xut73dq 65 13 be be VB cord-329061-1xut73dq 65 14 susceptible susceptible JJ cord-329061-1xut73dq 65 15 to to IN cord-329061-1xut73dq 65 16 SDA SDA NNP cord-329061-1xut73dq 65 17 virus virus NN cord-329061-1xut73dq 65 18 , , , cord-329061-1xut73dq 65 19 were be VBD cord-329061-1xut73dq 65 20 inoculated inoculate VBN cord-329061-1xut73dq 65 21 by by IN cord-329061-1xut73dq 65 22 the the DT cord-329061-1xut73dq 65 23 intranasal intranasal NN cord-329061-1xut73dq 65 24 route route NN cord-329061-1xut73dq 65 25 with with IN cord-329061-1xut73dq 65 26 fourth fourth JJ cord-329061-1xut73dq 65 27 passage passage NN cord-329061-1xut73dq 65 28 , , , cord-329061-1xut73dq 65 29 infectedmouse infectedmouse NN cord-329061-1xut73dq 65 30 - - HYPH cord-329061-1xut73dq 65 31 brain brain NN cord-329061-1xut73dq 65 32 material material NN cord-329061-1xut73dq 65 33 . . . cord-329061-1xut73dq 66 1 The the DT cord-329061-1xut73dq 66 2 inoculum inoculum NN cord-329061-1xut73dq 66 3 contained contain VBD cord-329061-1xut73dq 66 4 approximately approximately RB cord-329061-1xut73dq 66 5 6.6 6.6 CD cord-329061-1xut73dq 66 6 X x NN cord-329061-1xut73dq 66 7 10 10 CD cord-329061-1xut73dq 66 8 3 3 CD cord-329061-1xut73dq 66 9 . . . cord-329061-1xut73dq 67 1 8 8 CD cord-329061-1xut73dq 67 2 IMLD IMLD NNP cord-329061-1xut73dq 67 3 50 50 CD cord-329061-1xut73dq 67 4 of of IN cord-329061-1xut73dq 67 5 virus virus NN cord-329061-1xut73dq 67 6 . . . cord-329061-1xut73dq 68 1 Rats rat NNS cord-329061-1xut73dq 68 2 were be VBD cord-329061-1xut73dq 68 3 sacrificed sacrifice VBN cord-329061-1xut73dq 68 4 on on IN cord-329061-1xut73dq 68 5 the the DT cord-329061-1xut73dq 68 6 fifth fifth JJ cord-329061-1xut73dq 68 7 , , , cord-329061-1xut73dq 68 8 sixth sixth JJ cord-329061-1xut73dq 68 9 , , , cord-329061-1xut73dq 68 10 and and CC cord-329061-1xut73dq 68 11 eighth eighth JJ cord-329061-1xut73dq 68 12 days day NNS cord-329061-1xut73dq 68 13 after after IN cord-329061-1xut73dq 68 14 inoculation inoculation NN cord-329061-1xut73dq 68 15 . . . cord-329061-1xut73dq 69 1 Harderian harderian JJ cord-329061-1xut73dq 69 2 and and CC cord-329061-1xut73dq 69 3 submaxillary submaxillary JJ cord-329061-1xut73dq 69 4 glands gland NNS cord-329061-1xut73dq 69 5 were be VBD cord-329061-1xut73dq 69 6 processed process VBN cord-329061-1xut73dq 69 7 for for IN cord-329061-1xut73dq 69 8 isolation isolation NN cord-329061-1xut73dq 69 9 of of IN cord-329061-1xut73dq 69 10 virus virus NN cord-329061-1xut73dq 69 11 and and CC cord-329061-1xut73dq 69 12 histologic histologic JJ cord-329061-1xut73dq 69 13 examination examination NN cord-329061-1xut73dq 69 14 , , , cord-329061-1xut73dq 69 15 whereas whereas IN cord-329061-1xut73dq 69 16 parotid parotid JJ cord-329061-1xut73dq 69 17 gland gland NN cord-329061-1xut73dq 69 18 was be VBD cord-329061-1xut73dq 69 19 collected collect VBN cord-329061-1xut73dq 69 20 for for IN cord-329061-1xut73dq 69 21 histologic histologic JJ cord-329061-1xut73dq 69 22 examination examination NN cord-329061-1xut73dq 69 23 only only RB cord-329061-1xut73dq 69 24 . . . cord-329061-1xut73dq 70 1 Adaptation adaptation NN cord-329061-1xut73dq 70 2 to to IN cord-329061-1xut73dq 70 3 mice mouse NNS cord-329061-1xut73dq 70 4 and and CC cord-329061-1xut73dq 70 5 related relate VBN cord-329061-1xut73dq 70 6 observations observation NNS cord-329061-1xut73dq 70 7 . . . cord-329061-1xut73dq 71 1 A a DT cord-329061-1xut73dq 71 2 10 10 CD cord-329061-1xut73dq 71 3 % % NN cord-329061-1xut73dq 71 4 suspension suspension NN cord-329061-1xut73dq 71 5 of of IN cord-329061-1xut73dq 71 6 infected infected JJ cord-329061-1xut73dq 71 7 salivary salivary NN cord-329061-1xut73dq 71 8 glands gland NNS cord-329061-1xut73dq 71 9 was be VBD cord-329061-1xut73dq 71 10 inoculated inoculate VBN cord-329061-1xut73dq 71 11 ic ic NNP cord-329061-1xut73dq 71 12 into into IN cord-329061-1xut73dq 71 13 one one CD cord-329061-1xut73dq 71 14 - - HYPH cord-329061-1xut73dq 71 15 day day NN cord-329061-1xut73dq 71 16 - - HYPH cord-329061-1xut73dq 71 17 old old JJ cord-329061-1xut73dq 71 18 mice mouse NNS cord-329061-1xut73dq 71 19 . . . cord-329061-1xut73dq 72 1 One one CD cord-329061-1xut73dq 72 2 mouse mouse NN cord-329061-1xut73dq 72 3 was be VBD cord-329061-1xut73dq 72 4 sick sick JJ cord-329061-1xut73dq 72 5 on on IN cord-329061-1xut73dq 72 6 the the DT cord-329061-1xut73dq 72 7 fifth fifth JJ cord-329061-1xut73dq 72 8 day day NN cord-329061-1xut73dq 72 9 after after IN cord-329061-1xut73dq 72 10 inoculation inoculation NN cord-329061-1xut73dq 72 11 , , , cord-329061-1xut73dq 72 12 eight eight CD cord-329061-1xut73dq 72 13 more more JJR cord-329061-1xut73dq 72 14 were be VBD cord-329061-1xut73dq 72 15 sick sick JJ cord-329061-1xut73dq 72 16 on on IN cord-329061-1xut73dq 72 17 the the DT cord-329061-1xut73dq 72 18 seventh seventh JJ cord-329061-1xut73dq 72 19 day day NN cord-329061-1xut73dq 72 20 after after IN cord-329061-1xut73dq 72 21 inoculation inoculation NN cord-329061-1xut73dq 72 22 , , , cord-329061-1xut73dq 72 23 and and CC cord-329061-1xut73dq 72 24 six six CD cord-329061-1xut73dq 72 25 on on IN cord-329061-1xut73dq 72 26 the the DT cord-329061-1xut73dq 72 27 eighth eighth JJ cord-329061-1xut73dq 72 28 day day NN cord-329061-1xut73dq 72 29 after after IN cord-329061-1xut73dq 72 30 inoculation inoculation NN cord-329061-1xut73dq 72 31 . . . cord-329061-1xut73dq 73 1 Some some DT cord-329061-1xut73dq 73 2 of of IN cord-329061-1xut73dq 73 3 these these DT cord-329061-1xut73dq 73 4 animals animal NNS cord-329061-1xut73dq 73 5 were be VBD cord-329061-1xut73dq 73 6 killed kill VBN cord-329061-1xut73dq 73 7 , , , cord-329061-1xut73dq 73 8 and and CC cord-329061-1xut73dq 73 9 tissues tissue NNS cord-329061-1xut73dq 73 10 were be VBD cord-329061-1xut73dq 73 11 harvested harvest VBN cord-329061-1xut73dq 73 12 for for IN cord-329061-1xut73dq 73 13 passages passage NNS cord-329061-1xut73dq 73 14 and and CC cord-329061-1xut73dq 73 15 histologic histologic JJ cord-329061-1xut73dq 73 16 study study NN cord-329061-1xut73dq 73 17 , , , cord-329061-1xut73dq 73 18 but but CC cord-329061-1xut73dq 73 19 mice mouse NNS cord-329061-1xut73dq 73 20 that that WDT cord-329061-1xut73dq 73 21 were be VBD cord-329061-1xut73dq 73 22 sick sick JJ cord-329061-1xut73dq 73 23 but but CC cord-329061-1xut73dq 73 24 not not RB cord-329061-1xut73dq 73 25 killed kill VBN cord-329061-1xut73dq 73 26 died die VBD cord-329061-1xut73dq 73 27 on on IN cord-329061-1xut73dq 73 28 the the DT cord-329061-1xut73dq 73 29 10th 10th JJ cord-329061-1xut73dq 73 30 day day NN cord-329061-1xut73dq 73 31 after after IN cord-329061-1xut73dq 73 32 inoculation inoculation NN cord-329061-1xut73dq 73 33 . . . cord-329061-1xut73dq 74 1 One one CD cord-329061-1xut73dq 74 2 mouse mouse NN cord-329061-1xut73dq 74 3 was be VBD cord-329061-1xut73dq 74 4 unaffected unaffected JJ cord-329061-1xut73dq 74 5 and and CC cord-329061-1xut73dq 74 6 survived survive VBD cord-329061-1xut73dq 74 7 until until IN cord-329061-1xut73dq 74 8 the the DT cord-329061-1xut73dq 74 9 21st 21st JJ cord-329061-1xut73dq 74 10 day day NN cord-329061-1xut73dq 74 11 , , , cord-329061-1xut73dq 74 12 when when WRB cord-329061-1xut73dq 74 13 it -PRON- PRP cord-329061-1xut73dq 74 14 was be VBD cord-329061-1xut73dq 74 15 discarded discard VBN cord-329061-1xut73dq 74 16 . . . cord-329061-1xut73dq 75 1 The the DT cord-329061-1xut73dq 75 2 disease disease NN cord-329061-1xut73dq 75 3 was be VBD cord-329061-1xut73dq 75 4 characterized characterize VBN cord-329061-1xut73dq 75 5 by by IN cord-329061-1xut73dq 75 6 ataxia ataxia NN cord-329061-1xut73dq 75 7 and and CC cord-329061-1xut73dq 75 8 uncoordination uncoordination NN cord-329061-1xut73dq 75 9 , , , cord-329061-1xut73dq 75 10 followed follow VBN cord-329061-1xut73dq 75 11 by by IN cord-329061-1xut73dq 75 12 paresis paresis NN cord-329061-1xut73dq 75 13 , , , cord-329061-1xut73dq 75 14 paralysis paralysis NN cord-329061-1xut73dq 75 15 , , , cord-329061-1xut73dq 75 16 and and CC cord-329061-1xut73dq 75 17 death death NN cord-329061-1xut73dq 75 18 . . . cord-329061-1xut73dq 76 1 The the DT cord-329061-1xut73dq 76 2 same same JJ cord-329061-1xut73dq 76 3 pattern pattern NN cord-329061-1xut73dq 76 4 of of IN cord-329061-1xut73dq 76 5 illness illness NN cord-329061-1xut73dq 76 6 was be VBD cord-329061-1xut73dq 76 7 observed observe VBN cord-329061-1xut73dq 76 8 on on IN cord-329061-1xut73dq 76 9 further further JJ cord-329061-1xut73dq 76 10 passages passage NNS cord-329061-1xut73dq 76 11 . . . cord-329061-1xut73dq 77 1 By by IN cord-329061-1xut73dq 77 2 the the DT cord-329061-1xut73dq 77 3 fifth fifth JJ cord-329061-1xut73dq 77 4 mouse mouse NN cord-329061-1xut73dq 77 5 - - HYPH cord-329061-1xut73dq 77 6 brain brain NN cord-329061-1xut73dq 77 7 passage passage NN cord-329061-1xut73dq 77 8 , , , cord-329061-1xut73dq 77 9 the the DT cord-329061-1xut73dq 77 10 incubation incubation NN cord-329061-1xut73dq 77 11 period period NN cord-329061-1xut73dq 77 12 was be VBD cord-329061-1xut73dq 77 13 125 125 CD cord-329061-1xut73dq 77 14 shortened shorten VBN cord-329061-1xut73dq 77 15 to to IN cord-329061-1xut73dq 77 16 two two CD cord-329061-1xut73dq 77 17 to to TO cord-329061-1xut73dq 77 18 three three CD cord-329061-1xut73dq 77 19 days day NNS cord-329061-1xut73dq 77 20 . . . cord-329061-1xut73dq 78 1 There there EX cord-329061-1xut73dq 78 2 was be VBD cord-329061-1xut73dq 78 3 usually usually RB cord-329061-1xut73dq 78 4 a a DT cord-329061-1xut73dq 78 5 random random JJ cord-329061-1xut73dq 78 6 pattern pattern NN cord-329061-1xut73dq 78 7 of of IN cord-329061-1xut73dq 78 8 illness illness NN cord-329061-1xut73dq 78 9 and and CC cord-329061-1xut73dq 78 10 death death NN cord-329061-1xut73dq 78 11 from from IN cord-329061-1xut73dq 78 12 two two CD cord-329061-1xut73dq 78 13 to to TO cord-329061-1xut73dq 78 14 eight eight CD cord-329061-1xut73dq 78 15 days day NNS cord-329061-1xut73dq 78 16 and and CC cord-329061-1xut73dq 78 17 occasionally occasionally RB cord-329061-1xut73dq 78 18 up up IN cord-329061-1xut73dq 78 19 to to TO cord-329061-1xut73dq 78 20 10 10 CD cord-329061-1xut73dq 78 21 days day NNS cord-329061-1xut73dq 78 22 after after IN cord-329061-1xut73dq 78 23 inoculation inoculation NN cord-329061-1xut73dq 78 24 . . . cord-329061-1xut73dq 79 1 The the DT cord-329061-1xut73dq 79 2 pattern pattern NN cord-329061-1xut73dq 79 3 has have VBZ cord-329061-1xut73dq 79 4 remained remain VBN cord-329061-1xut73dq 79 5 unchanged unchanged JJ cord-329061-1xut73dq 79 6 for for IN cord-329061-1xut73dq 79 7 29 29 CD cord-329061-1xut73dq 79 8 passages passage NNS cord-329061-1xut73dq 79 9 with with IN cord-329061-1xut73dq 79 10 this this DT cord-329061-1xut73dq 79 11 strain strain NN cord-329061-1xut73dq 79 12 of of IN cord-329061-1xut73dq 79 13 virus virus NN cord-329061-1xut73dq 79 14 . . . cord-329061-1xut73dq 80 1 One one CD cord-329061-1xut73dq 80 2 other other JJ cord-329061-1xut73dq 80 3 observation observation NN cord-329061-1xut73dq 80 4 made make VBN cord-329061-1xut73dq 80 5 during during IN cord-329061-1xut73dq 80 6 the the DT cord-329061-1xut73dq 80 7 first first JJ cord-329061-1xut73dq 80 8 passage passage NN cord-329061-1xut73dq 80 9 in in IN cord-329061-1xut73dq 80 10 mice mouse NNS cord-329061-1xut73dq 80 11 and and CC cord-329061-1xut73dq 80 12 amply amply RB cord-329061-1xut73dq 80 13 confirmed confirm VBD cord-329061-1xut73dq 80 14 during during IN cord-329061-1xut73dq 80 15 subsequent subsequent JJ cord-329061-1xut73dq 80 16 work work NN cord-329061-1xut73dq 80 17 was be VBD cord-329061-1xut73dq 80 18 emaciation emaciation NN cord-329061-1xut73dq 80 19 of of IN cord-329061-1xut73dq 80 20 sick sick JJ cord-329061-1xut73dq 80 21 mice mouse NNS cord-329061-1xut73dq 80 22 as as IN cord-329061-1xut73dq 80 23 compared compare VBN cord-329061-1xut73dq 80 24 to to IN cord-329061-1xut73dq 80 25 uninoculated uninoculated JJ cord-329061-1xut73dq 80 26 control control NN cord-329061-1xut73dq 80 27 mice mouse NNS cord-329061-1xut73dq 80 28 of of IN cord-329061-1xut73dq 80 29 the the DT cord-329061-1xut73dq 80 30 same same JJ cord-329061-1xut73dq 80 31 age age NN cord-329061-1xut73dq 80 32 . . . cord-329061-1xut73dq 81 1 These these DT cord-329061-1xut73dq 81 2 differences difference NNS cord-329061-1xut73dq 81 3 were be VBD cord-329061-1xut73dq 81 4 more more RBR cord-329061-1xut73dq 81 5 marked mark VBN cord-329061-1xut73dq 81 6 in in IN cord-329061-1xut73dq 81 7 mice mouse NNS cord-329061-1xut73dq 81 8 that that WDT cord-329061-1xut73dq 81 9 were be VBD cord-329061-1xut73dq 81 10 two two CD cord-329061-1xut73dq 81 11 to to TO cord-329061-1xut73dq 81 12 four four CD cord-329061-1xut73dq 81 13 days day NNS cord-329061-1xut73dq 81 14 old old JJ cord-329061-1xut73dq 81 15 or or CC cord-329061-1xut73dq 81 16 older old JJR cord-329061-1xut73dq 81 17 when when WRB cord-329061-1xut73dq 81 18 inoculated inoculate VBN cord-329061-1xut73dq 81 19 . . . cord-329061-1xut73dq 82 1 Other other JJ cord-329061-1xut73dq 82 2 significant significant JJ cord-329061-1xut73dq 82 3 observations observation NNS cord-329061-1xut73dq 82 4 can can MD cord-329061-1xut73dq 82 5 be be VB cord-329061-1xut73dq 82 6 summarized summarize VBN cord-329061-1xut73dq 82 7 as as IN cord-329061-1xut73dq 82 8 follows follow VBZ cord-329061-1xut73dq 82 9 : : : cord-329061-1xut73dq 83 1 ( ( -LRB- cord-329061-1xut73dq 83 2 l l NN cord-329061-1xut73dq 83 3 ) ) -RRB- cord-329061-1xut73dq 83 4 The the DT cord-329061-1xut73dq 83 5 agent agent NN cord-329061-1xut73dq 83 6 of of IN cord-329061-1xut73dq 83 7 SDA SDA NNP cord-329061-1xut73dq 83 8 does do VBZ cord-329061-1xut73dq 83 9 not not RB cord-329061-1xut73dq 83 10 cause cause VB cord-329061-1xut73dq 83 11 detectable detectable JJ cord-329061-1xut73dq 83 12 illness illness NN cord-329061-1xut73dq 83 13 in in IN cord-329061-1xut73dq 83 14 weanling weanle VBG cord-329061-1xut73dq 83 15 ( ( -LRB- cord-329061-1xut73dq 83 16 three three CD cord-329061-1xut73dq 83 17 - - HYPH cord-329061-1xut73dq 83 18 to to TO cord-329061-1xut73dq 83 19 four four CD cord-329061-1xut73dq 83 20 - - HYPH cord-329061-1xut73dq 83 21 week week NN cord-329061-1xut73dq 83 22 - - HYPH cord-329061-1xut73dq 83 23 old old JJ cord-329061-1xut73dq 83 24 ) ) -RRB- cord-329061-1xut73dq 83 25 mice mouse NNS cord-329061-1xut73dq 83 26 when when WRB cord-329061-1xut73dq 83 27 inoculated inoculate VBN cord-329061-1xut73dq 83 28 ic ic NNP cord-329061-1xut73dq 83 29 or or CC cord-329061-1xut73dq 83 30 ip ip NN cord-329061-1xut73dq 83 31 or or CC cord-329061-1xut73dq 83 32 in in IN cord-329061-1xut73dq 83 33 infant infant NN cord-329061-1xut73dq 83 34 mice mouse NNS cord-329061-1xut73dq 83 35 inoculated inoculate VBD cord-329061-1xut73dq 83 36 ip ip NN cord-329061-1xut73dq 83 37 . . . cord-329061-1xut73dq 84 1 ( ( -LRB- cord-329061-1xut73dq 84 2 2 2 LS cord-329061-1xut73dq 84 3 ) ) -RRB- cord-329061-1xut73dq 85 1 A a DT cord-329061-1xut73dq 85 2 comparative comparative JJ cord-329061-1xut73dq 85 3 titration titration NN cord-329061-1xut73dq 85 4 was be VBD cord-329061-1xut73dq 85 5 done do VBN cord-329061-1xut73dq 85 6 in in IN cord-329061-1xut73dq 85 7 mice mouse NNS cord-329061-1xut73dq 85 8 two two CD cord-329061-1xut73dq 85 9 days day NNS cord-329061-1xut73dq 85 10 old old JJ cord-329061-1xut73dq 85 11 , , , cord-329061-1xut73dq 85 12 13 13 CD cord-329061-1xut73dq 85 13 days day NNS cord-329061-1xut73dq 85 14 old old JJ cord-329061-1xut73dq 85 15 , , , cord-329061-1xut73dq 85 16 and and CC cord-329061-1xut73dq 85 17 22 22 CD cord-329061-1xut73dq 85 18 days day NNS cord-329061-1xut73dq 85 19 old old JJ cord-329061-1xut73dq 86 1 that that WDT cord-329061-1xut73dq 86 2 were be VBD cord-329061-1xut73dq 86 3 inoculated inoculate VBN cord-329061-1xut73dq 86 4 ic ic NNP cord-329061-1xut73dq 86 5 with with IN cord-329061-1xut73dq 86 6 viral viral JJ cord-329061-1xut73dq 86 7 stock stock NN cord-329061-1xut73dq 86 8 passaged passage VBD cord-329061-1xut73dq 86 9 12 12 CD cord-329061-1xut73dq 86 10 times time NNS cord-329061-1xut73dq 86 11 in in IN cord-329061-1xut73dq 86 12 mouse mouse NN cord-329061-1xut73dq 86 13 brain brain NN cord-329061-1xut73dq 86 14 . . . cord-329061-1xut73dq 87 1 Titers titer NNS cord-329061-1xut73dq 87 2 were be VBD cord-329061-1xut73dq 87 3 10 10 CD cord-329061-1xut73dq 87 4 4 4 CD cord-329061-1xut73dq 87 5 . . . cord-329061-1xut73dq 87 6 00 00 NFP cord-329061-1xut73dq 87 7 , , , cord-329061-1xut73dq 87 8 10 10 CD cord-329061-1xut73dq 87 9 4 4 CD cord-329061-1xut73dq 87 10 . . . cord-329061-1xut73dq 87 11 25 25 CD cord-329061-1xut73dq 87 12 , , , cord-329061-1xut73dq 87 13 and and CC cord-329061-1xut73dq 87 14 < < XX cord-329061-1xut73dq 87 15 10 10 CD cord-329061-1xut73dq 87 16 2 2 CD cord-329061-1xut73dq 87 17 . . . cord-329061-1xut73dq 87 18 0 0 NFP cord-329061-1xut73dq 88 1 IMLD50/0.015 IMLD50/0.015 NNP cord-329061-1xut73dq 88 2 mI mI NNP cord-329061-1xut73dq 88 3 , , , cord-329061-1xut73dq 88 4 respectively respectively RB cord-329061-1xut73dq 88 5 . . . cord-329061-1xut73dq 89 1 ( ( -LRB- cord-329061-1xut73dq 89 2 3 3 LS cord-329061-1xut73dq 89 3 ) ) -RRB- cord-329061-1xut73dq 89 4 Virus Virus NNP cord-329061-1xut73dq 89 5 has have VBZ cord-329061-1xut73dq 89 6 undergone undergo VBN cord-329061-1xut73dq 89 7 29 29 CD cord-329061-1xut73dq 89 8 serial serial JJ cord-329061-1xut73dq 89 9 ic ic NN cord-329061-1xut73dq 89 10 passages passage NNS cord-329061-1xut73dq 89 11 in in IN cord-329061-1xut73dq 89 12 zero zero CD cord-329061-1xut73dq 89 13 - - HYPH cord-329061-1xut73dq 89 14 to to TO cord-329061-1xut73dq 89 15 six six CD cord-329061-1xut73dq 89 16 - - HYPH cord-329061-1xut73dq 89 17 day day NN cord-329061-1xut73dq 89 18 - - HYPH cord-329061-1xut73dq 89 19 old old JJ cord-329061-1xut73dq 89 20 mice mouse NNS cord-329061-1xut73dq 89 21 , , , cord-329061-1xut73dq 89 22 the the DT cord-329061-1xut73dq 89 23 cumulative cumulative JJ cord-329061-1xut73dq 89 24 dilution dilution NN cord-329061-1xut73dq 89 25 of of IN cord-329061-1xut73dq 89 26 which which WDT cord-329061-1xut73dq 89 27 exceeds exceed VBZ cord-329061-1xut73dq 89 28 10 10 CD cord-329061-1xut73dq 89 29 - - SYM cord-329061-1xut73dq 89 30 100 100 CD cord-329061-1xut73dq 89 31 . . . cord-329061-1xut73dq 90 1 ( ( -LRB- cord-329061-1xut73dq 90 2 4 4 LS cord-329061-1xut73dq 90 3 ) ) -RRB- cord-329061-1xut73dq 91 1 The the DT cord-329061-1xut73dq 91 2 titer titer NN cord-329061-1xut73dq 91 3 of of IN cord-329061-1xut73dq 91 4 virus virus NN cord-329061-1xut73dq 91 5 between between IN cord-329061-1xut73dq 91 6 the the DT cord-329061-1xut73dq 91 7 fifth fifth JJ cord-329061-1xut73dq 91 8 and and CC cord-329061-1xut73dq 91 9 29th 29th JJ cord-329061-1xut73dq 91 10 passage passage NN cord-329061-1xut73dq 91 11 in in IN cord-329061-1xut73dq 91 12 mouse mouse NN cord-329061-1xut73dq 91 13 brain brain NN cord-329061-1xut73dq 91 14 has have VBZ cord-329061-1xut73dq 91 15 remained remain VBN cord-329061-1xut73dq 91 16 relatively relatively RB cord-329061-1xut73dq 91 17 stable stable JJ cord-329061-1xut73dq 91 18 at at IN cord-329061-1xut73dq 91 19 10 10 CD cord-329061-1xut73dq 91 20 3 3 CD cord-329061-1xut73dq 91 21 . . . cord-329061-1xut73dq 92 1 5_105.0 5_105.0 CD cord-329061-1xut73dq 92 2 IMLD50/0.015 IMLD50/0.015 NNP cord-329061-1xut73dq 92 3 ml ml NN cord-329061-1xut73dq 92 4 ( ( -LRB- cord-329061-1xut73dq 92 5 usually usually RB cord-329061-1xut73dq 92 6 around around IN cord-329061-1xut73dq 92 7 1Q3·7 1q3·7 CD cord-329061-1xut73dq 92 8 IMLD50/0.015 IMLD50/0.015 NNP cord-329061-1xut73dq 92 9 ml ml NN cord-329061-1xut73dq 92 10 ) ) -RRB- cord-329061-1xut73dq 92 11 . . . cord-329061-1xut73dq 93 1 ( ( -LRB- cord-329061-1xut73dq 93 2 5 5 LS cord-329061-1xut73dq 93 3 ) ) -RRB- cord-329061-1xut73dq 94 1 The the DT cord-329061-1xut73dq 94 2 original original JJ cord-329061-1xut73dq 94 3 salivary salivary NN cord-329061-1xut73dq 94 4 - - HYPH cord-329061-1xut73dq 94 5 gland gland NN cord-329061-1xut73dq 94 6 suspension suspension NN cord-329061-1xut73dq 94 7 was be VBD cord-329061-1xut73dq 94 8 titrated titrate VBN cord-329061-1xut73dq 94 9 in in IN cord-329061-1xut73dq 94 10 one one CD cord-329061-1xut73dq 94 11 - - HYPH cord-329061-1xut73dq 94 12 day day NN cord-329061-1xut73dq 94 13 - - HYPH cord-329061-1xut73dq 94 14 old old JJ cord-329061-1xut73dq 94 15 mice mouse NNS cord-329061-1xut73dq 94 16 and and CC cord-329061-1xut73dq 94 17 had have VBD cord-329061-1xut73dq 94 18 a a DT cord-329061-1xut73dq 94 19 titer titer NN cord-329061-1xut73dq 94 20 of of IN cord-329061-1xut73dq 94 21 1()3·6 1()3·6 CD cord-329061-1xut73dq 94 22 IMLD IMLD NNP cord-329061-1xut73dq 94 23 50 50 CD cord-329061-1xut73dq 94 24 / / SYM cord-329061-1xut73dq 94 25 O.015 O.015 NNP cord-329061-1xut73dq 94 26 ml ml XX cord-329061-1xut73dq 94 27 . . . cord-329061-1xut73dq 95 1 ( ( -LRB- cord-329061-1xut73dq 95 2 6 6 CD cord-329061-1xut73dq 95 3 ) ) -RRB- cord-329061-1xut73dq 96 1 When when WRB cord-329061-1xut73dq 96 2 inoculated inoculate VBN cord-329061-1xut73dq 96 3 intranasally intranasally RB cord-329061-1xut73dq 96 4 into into IN cord-329061-1xut73dq 96 5 susceptible susceptible JJ cord-329061-1xut73dq 96 6 rats rat NNS cord-329061-1xut73dq 96 7 , , , cord-329061-1xut73dq 96 8 mouse mouse NN cord-329061-1xut73dq 96 9 - - HYPH cord-329061-1xut73dq 96 10 brain brain NN cord-329061-1xut73dq 96 11 - - HYPH cord-329061-1xut73dq 96 12 adapted adapt VBN cord-329061-1xut73dq 96 13 virus virus NN cord-329061-1xut73dq 96 14 produced produce VBN cord-329061-1xut73dq 96 15 sialodacryoadenitis sialodacryoadenitis NNP cord-329061-1xut73dq 96 16 . . . cord-329061-1xut73dq 97 1 ( ( -LRB- cord-329061-1xut73dq 97 2 7 7 LS cord-329061-1xut73dq 97 3 ) ) -RRB- cord-329061-1xut73dq 97 4 Brains brain NNS cord-329061-1xut73dq 97 5 from from IN cord-329061-1xut73dq 97 6 two two CD cord-329061-1xut73dq 97 7 uninoculated uninoculated JJ cord-329061-1xut73dq 97 8 mice mouse NNS cord-329061-1xut73dq 97 9 ( ( -LRB- cord-329061-1xut73dq 97 10 two two CD cord-329061-1xut73dq 97 11 days day NNS cord-329061-1xut73dq 97 12 old old JJ cord-329061-1xut73dq 97 13 ) ) -RRB- cord-329061-1xut73dq 97 14 were be VBD cord-329061-1xut73dq 97 15 harvested harvest VBN cord-329061-1xut73dq 97 16 as as IN cord-329061-1xut73dq 97 17 controls control NNS cord-329061-1xut73dq 97 18 ; ; : cord-329061-1xut73dq 97 19 seven seven CD cord-329061-1xut73dq 97 20 serial serial JJ cord-329061-1xut73dq 97 21 ic ic NN cord-329061-1xut73dq 97 22 passages passage NNS cord-329061-1xut73dq 97 23 of of IN cord-329061-1xut73dq 97 24 this this DT cord-329061-1xut73dq 97 25 material material NN cord-329061-1xut73dq 97 26 were be VBD cord-329061-1xut73dq 97 27 made make VBN cord-329061-1xut73dq 97 28 at at IN cord-329061-1xut73dq 97 29 intervals interval NNS cord-329061-1xut73dq 97 30 of of IN cord-329061-1xut73dq 97 31 six six CD cord-329061-1xut73dq 97 32 to to TO cord-329061-1xut73dq 97 33 seven seven CD cord-329061-1xut73dq 97 34 days day NNS cord-329061-1xut73dq 97 35 in in IN cord-329061-1xut73dq 97 36 mice mouse NNS cord-329061-1xut73dq 97 37 three three CD cord-329061-1xut73dq 97 38 to to TO cord-329061-1xut73dq 97 39 four four CD cord-329061-1xut73dq 97 40 days day NNS cord-329061-1xut73dq 97 41 old old JJ cord-329061-1xut73dq 97 42 . . . cord-329061-1xut73dq 98 1 No no DT cord-329061-1xut73dq 98 2 agent agent NN cord-329061-1xut73dq 98 3 pathogenic pathogenic JJ cord-329061-1xut73dq 98 4 for for IN cord-329061-1xut73dq 98 5 mice mouse NNS cord-329061-1xut73dq 98 6 was be VBD cord-329061-1xut73dq 98 7 isolated isolate VBN cord-329061-1xut73dq 98 8 from from IN cord-329061-1xut73dq 98 9 these these DT cord-329061-1xut73dq 98 10 control control NN cord-329061-1xut73dq 98 11 animals animal NNS cord-329061-1xut73dq 98 12 . . . cord-329061-1xut73dq 99 1 Histopathologic Histopathologic NNP cord-329061-1xut73dq 99 2 and and CC cord-329061-1xut73dq 99 3 immunofluorescent immunofluorescent NN cord-329061-1xut73dq 99 4 observations observation NNS cord-329061-1xut73dq 99 5 in in IN cord-329061-1xut73dq 99 6 inoculated inoculate VBN cord-329061-1xut73dq 99 7 mice mouse NNS cord-329061-1xut73dq 99 8 . . . cord-329061-1xut73dq 100 1 In in IN cord-329061-1xut73dq 100 2 general general JJ cord-329061-1xut73dq 100 3 , , , cord-329061-1xut73dq 100 4 histologic histologic JJ cord-329061-1xut73dq 100 5 changes change NNS cord-329061-1xut73dq 100 6 observed observe VBN cord-329061-1xut73dq 100 7 in in IN cord-329061-1xut73dq 100 8 the the DT cord-329061-1xut73dq 100 9 central central JJ cord-329061-1xut73dq 100 10 nervous nervous JJ cord-329061-1xut73dq 100 11 system system NN cord-329061-1xut73dq 100 12 of of IN cord-329061-1xut73dq 100 13 inoculated inoculate VBN cord-329061-1xut73dq 100 14 mice mouse NNS cord-329061-1xut73dq 100 15 were be VBD cord-329061-1xut73dq 100 16 characterized characterize VBN cord-329061-1xut73dq 100 17 by by IN cord-329061-1xut73dq 100 18 diffuse diffuse JJ cord-329061-1xut73dq 100 19 and and CC cord-329061-1xut73dq 100 20 focal focal JJ cord-329061-1xut73dq 100 21 neuronal neuronal JJ cord-329061-1xut73dq 100 22 degeneration degeneration NN cord-329061-1xut73dq 100 23 with with IN cord-329061-1xut73dq 100 24 minimal minimal JJ cord-329061-1xut73dq 100 25 inflammatory inflammatory JJ cord-329061-1xut73dq 100 26 cell cell NN cord-329061-1xut73dq 100 27 response response NN cord-329061-1xut73dq 100 28 . . . cord-329061-1xut73dq 101 1 Regions region NNS cord-329061-1xut73dq 101 2 of of IN cord-329061-1xut73dq 101 3 brain brain NN cord-329061-1xut73dq 101 4 most most RBS cord-329061-1xut73dq 101 5 frequently frequently RB cord-329061-1xut73dq 101 6 involved involve VBN cord-329061-1xut73dq 101 7 were be VBD cord-329061-1xut73dq 101 8 the the DT cord-329061-1xut73dq 101 9 cortices cortex NNS cord-329061-1xut73dq 101 10 of of IN cord-329061-1xut73dq 101 11 the the DT cord-329061-1xut73dq 101 12 occipital occipital JJ cord-329061-1xut73dq 101 13 and and CC cord-329061-1xut73dq 101 14 parietal parietal JJ cord-329061-1xut73dq 101 15 lobes lobe NNS cord-329061-1xut73dq 101 16 . . . cord-329061-1xut73dq 102 1 Other other JJ cord-329061-1xut73dq 102 2 foci focus NNS cord-329061-1xut73dq 102 3 of of IN cord-329061-1xut73dq 102 4 neuronal neuronal JJ cord-329061-1xut73dq 102 5 destruction destruction NN cord-329061-1xut73dq 102 6 were be VBD cord-329061-1xut73dq 102 7 scattered scatter VBN cord-329061-1xut73dq 102 8 elsewhere elsewhere RB cord-329061-1xut73dq 102 9 in in IN cord-329061-1xut73dq 102 10 the the DT cord-329061-1xut73dq 102 11 central central JJ cord-329061-1xut73dq 102 12 nervous nervous JJ cord-329061-1xut73dq 102 13 system system NN cord-329061-1xut73dq 102 14 ; ; : cord-329061-1xut73dq 102 15 there there EX cord-329061-1xut73dq 102 16 was be VBD cord-329061-1xut73dq 102 17 relatively relatively RB cord-329061-1xut73dq 102 18 little little JJ cord-329061-1xut73dq 102 19 destruction destruction NN cord-329061-1xut73dq 102 20 0 0 NFP cord-329061-1xut73dq 102 21 _SP cord-329061-1xut73dq 103 1 Some some DT cord-329061-1xut73dq 103 2 important important JJ cord-329061-1xut73dq 103 3 observations observation NNS cord-329061-1xut73dq 103 4 are be VBP cord-329061-1xut73dq 103 5 summarized summarize VBN cord-329061-1xut73dq 103 6 as as IN cord-329061-1xut73dq 103 7 follows follow VBZ cord-329061-1xut73dq 103 8 . . . cord-329061-1xut73dq 104 1 ( ( -LRB- cord-329061-1xut73dq 104 2 l l NN cord-329061-1xut73dq 104 3 ) ) -RRB- cord-329061-1xut73dq 104 4 Cultures culture NNS cord-329061-1xut73dq 104 5 were be VBD cord-329061-1xut73dq 104 6 most most RBS cord-329061-1xut73dq 104 7 sensitive sensitive JJ cord-329061-1xut73dq 104 8 when when WRB cord-329061-1xut73dq 104 9 used use VBN cord-329061-1xut73dq 104 10 within within IN cord-329061-1xut73dq 104 11 a a DT cord-329061-1xut73dq 104 12 week week NN cord-329061-1xut73dq 104 13 after after IN cord-329061-1xut73dq 104 14 seeding seed VBG cord-329061-1xut73dq 104 15 ; ; : cord-329061-1xut73dq 104 16 then then RB cord-329061-1xut73dq 104 17 sensitivity sensitivity NN cord-329061-1xut73dq 104 18 decreased decrease VBD cord-329061-1xut73dq 104 19 . . . cord-329061-1xut73dq 105 1 The the DT cord-329061-1xut73dq 105 2 CPE CPE NNP cord-329061-1xut73dq 105 3 was be VBD cord-329061-1xut73dq 105 4 delayed delay VBN cord-329061-1xut73dq 105 5 and and CC cord-329061-1xut73dq 105 6 less less RBR cord-329061-1xut73dq 105 7 extensive extensive JJ cord-329061-1xut73dq 105 8 in in IN cord-329061-1xut73dq 105 9 older old JJR cord-329061-1xut73dq 105 10 cultures culture NNS cord-329061-1xut73dq 105 11 . . . cord-329061-1xut73dq 106 1 ( ( -LRB- cord-329061-1xut73dq 106 2 2 2 LS cord-329061-1xut73dq 106 3 ) ) -RRB- cord-329061-1xut73dq 106 4 Development development NN cord-329061-1xut73dq 106 5 of of IN cord-329061-1xut73dq 106 6 virus virus NN cord-329061-1xut73dq 106 7 in in IN cord-329061-1xut73dq 106 8 PRK PRK NNP cord-329061-1xut73dq 106 9 cells cell NNS cord-329061-1xut73dq 106 10 was be VBD cord-329061-1xut73dq 106 11 monitored monitor VBN cord-329061-1xut73dq 106 12 by by IN cord-329061-1xut73dq 106 13 CPE CPE NNP cord-329061-1xut73dq 106 14 , , , cord-329061-1xut73dq 106 15 detection detection NN cord-329061-1xut73dq 106 16 of of IN cord-329061-1xut73dq 106 17 viral viral JJ cord-329061-1xut73dq 106 18 antigen antigen NN cord-329061-1xut73dq 106 19 by by IN cord-329061-1xut73dq 106 20 indirect indirect JJ cord-329061-1xut73dq 106 21 immunofluorescence immunofluorescence NN cord-329061-1xut73dq 106 22 , , , cord-329061-1xut73dq 106 23 and and CC cord-329061-1xut73dq 106 24 quantitation quantitation NN cord-329061-1xut73dq 106 25 of of IN cord-329061-1xut73dq 106 26 infectious infectious JJ cord-329061-1xut73dq 106 27 virus virus NN cord-329061-1xut73dq 106 28 in in IN cord-329061-1xut73dq 106 29 PRK PRK NNP cord-329061-1xut73dq 106 30 tubes tube NNS cord-329061-1xut73dq 106 31 . . . cord-329061-1xut73dq 107 1 Results result NNS cord-329061-1xut73dq 107 2 are be VBP cord-329061-1xut73dq 107 3 presented present VBN cord-329061-1xut73dq 107 4 in in IN cord-329061-1xut73dq 107 5 table table NN cord-329061-1xut73dq 107 6 1 1 CD cord-329061-1xut73dq 107 7 . . . cord-329061-1xut73dq 108 1 Significantly significantly RB cord-329061-1xut73dq 108 2 , , , cord-329061-1xut73dq 108 3 detectable detectable JJ cord-329061-1xut73dq 108 4 viral viral JJ cord-329061-1xut73dq 108 5 antigen antigen NN cord-329061-1xut73dq 108 6 developed develop VBN cord-329061-1xut73dq 108 7 by by IN cord-329061-1xut73dq 108 8 12 12 CD cord-329061-1xut73dq 108 9 hr hr NN cord-329061-1xut73dq 108 10 and and CC cord-329061-1xut73dq 108 11 was be VBD cord-329061-1xut73dq 108 12 followed follow VBN cord-329061-1xut73dq 108 13 by by IN cord-329061-1xut73dq 108 14 release release NN cord-329061-1xut73dq 108 15 of of IN cord-329061-1xut73dq 108 16 infectious infectious JJ cord-329061-1xut73dq 108 17 virus virus NN cord-329061-1xut73dq 108 18 into into IN cord-329061-1xut73dq 108 19 the the DT cord-329061-1xut73dq 108 20 medium medium NN cord-329061-1xut73dq 108 21 . . . cord-329061-1xut73dq 109 1 CPE CPE NNP cord-329061-1xut73dq 109 2 was be VBD cord-329061-1xut73dq 109 3 detected detect VBN cord-329061-1xut73dq 109 4 at at IN cord-329061-1xut73dq 109 5 24 24 CD cord-329061-1xut73dq 109 6 hr hr NN cord-329061-1xut73dq 109 7 . . . cord-329061-1xut73dq 110 1 Beyond beyond IN cord-329061-1xut73dq 110 2 24 24 CD cord-329061-1xut73dq 110 3 hr hr NN cord-329061-1xut73dq 110 4 , , , cord-329061-1xut73dq 110 5 quantitation quantitation NN cord-329061-1xut73dq 110 6 of of IN cord-329061-1xut73dq 110 7 viral viral JJ cord-329061-1xut73dq 110 8 antigen antigen NN cord-329061-1xut73dq 110 9 was be VBD cord-329061-1xut73dq 110 10 difficult difficult JJ cord-329061-1xut73dq 110 11 due due IN cord-329061-1xut73dq 110 12 to to IN cord-329061-1xut73dq 110 13 lysis lysis NN cord-329061-1xut73dq 110 14 of of IN cord-329061-1xut73dq 110 15 cell cell NN cord-329061-1xut73dq 110 16 sheets sheet NNS cord-329061-1xut73dq 110 17 , , , cord-329061-1xut73dq 110 18 and and CC cord-329061-1xut73dq 110 19 after after IN cord-329061-1xut73dq 110 20 36 36 CD cord-329061-1xut73dq 110 21 hr hr NN cord-329061-1xut73dq 110 22 , , , cord-329061-1xut73dq 110 23 titer titer NN cord-329061-1xut73dq 110 24 of of IN cord-329061-1xut73dq 110 25 infectious infectious JJ cord-329061-1xut73dq 110 26 virus virus NN cord-329061-1xut73dq 110 27 decreased decrease VBD cord-329061-1xut73dq 110 28 . . . cord-329061-1xut73dq 111 1 ( ( -LRB- cord-329061-1xut73dq 111 2 3 3 LS cord-329061-1xut73dq 111 3 ) ) -RRB- cord-329061-1xut73dq 112 1 The the DT cord-329061-1xut73dq 112 2 sensitivity sensitivity NN cord-329061-1xut73dq 112 3 of of IN cord-329061-1xut73dq 112 4 inoculation inoculation NN cord-329061-1xut73dq 112 5 of of IN cord-329061-1xut73dq 112 6 mice mouse NNS cord-329061-1xut73dq 112 7 ic ic NNP cord-329061-1xut73dq 112 8 with with IN cord-329061-1xut73dq 112 9 strain strain NN cord-329061-1xut73dq 112 10 681 681 CD cord-329061-1xut73dq 112 11 virus virus NN cord-329061-1xut73dq 112 12 was be VBD cord-329061-1xut73dq 112 13 compared compare VBN cord-329061-1xut73dq 112 14 with with IN cord-329061-1xut73dq 112 15 that that DT cord-329061-1xut73dq 112 16 of of IN cord-329061-1xut73dq 112 17 inoculation inoculation NN cord-329061-1xut73dq 112 18 of of IN cord-329061-1xut73dq 112 19 PRK PRK NNP cord-329061-1xut73dq 112 20 cultures culture NNS cord-329061-1xut73dq 112 21 . . . cord-329061-1xut73dq 113 1 Titers titer NNS cord-329061-1xut73dq 113 2 obtained obtain VBD cord-329061-1xut73dq 113 3 with with IN cord-329061-1xut73dq 113 4 a a DT cord-329061-1xut73dq 113 5 mouse mouse NN cord-329061-1xut73dq 113 6 - - HYPH cord-329061-1xut73dq 113 7 brain brain NN cord-329061-1xut73dq 113 8 - - HYPH cord-329061-1xut73dq 113 9 adapted adapt VBN cord-329061-1xut73dq 113 10 virus virus NN cord-329061-1xut73dq 113 11 were be VBD cord-329061-1xut73dq 113 12 2.5 2.5 CD cord-329061-1xut73dq 113 13 X X NNS cord-329061-1xut73dq 113 14 10 10 CD cord-329061-1xut73dq 113 15 4 4 CD cord-329061-1xut73dq 113 16 IMLD50 imld50 CD cord-329061-1xut73dq 113 17 in in IN cord-329061-1xut73dq 113 18 mice mouse NNS cord-329061-1xut73dq 113 19 and and CC cord-329061-1xut73dq 113 20 lOX lox NN cord-329061-1xut73dq 113 21 10 10 CD cord-329061-1xut73dq 113 22 4 4 CD cord-329061-1xut73dq 113 23 . . . cord-329061-1xut73dq 114 1 3 3 LS cord-329061-1xut73dq 114 2 TCID50 TCID50 NNP cord-329061-1xut73dq 114 3 in in IN cord-329061-1xut73dq 114 4 PRK PRK NNP cord-329061-1xut73dq 114 5 cultures culture NNS cord-329061-1xut73dq 114 6 . . . cord-329061-1xut73dq 115 1 Similar similar JJ cord-329061-1xut73dq 115 2 differences difference NNS cord-329061-1xut73dq 115 3 were be VBD cord-329061-1xut73dq 115 4 also also RB cord-329061-1xut73dq 115 5 noted note VBN cord-329061-1xut73dq 115 6 in in IN cord-329061-1xut73dq 115 7 other other JJ cord-329061-1xut73dq 115 8 experiments experiment NNS cord-329061-1xut73dq 115 9 . . . cord-329061-1xut73dq 116 1 Characterization characterization NN cord-329061-1xut73dq 116 2 of of IN cord-329061-1xut73dq 116 3 the the DT cord-329061-1xut73dq 116 4 agent agent NN cord-329061-1xut73dq 116 5 . . . cord-329061-1xut73dq 117 1 The the DT cord-329061-1xut73dq 117 2 effect effect NN cord-329061-1xut73dq 117 3 of of IN cord-329061-1xut73dq 117 4 5-BUDR 5-budr CD cord-329061-1xut73dq 117 5 on on IN cord-329061-1xut73dq 117 6 viral viral JJ cord-329061-1xut73dq 117 7 multiplication multiplication NN cord-329061-1xut73dq 117 8 was be VBD cord-329061-1xut73dq 117 9 determined determine VBN cord-329061-1xut73dq 117 10 by by IN cord-329061-1xut73dq 117 11 the the DT cord-329061-1xut73dq 117 12 method method NN cord-329061-1xut73dq 117 13 of of IN cord-329061-1xut73dq 117 14 Bhatt Bhatt NNP cord-329061-1xut73dq 117 15 et et NNP cord-329061-1xut73dq 117 16 al al NNP cord-329061-1xut73dq 117 17 . . . cord-329061-1xut73dq 118 1 [ [ -LRB- cord-329061-1xut73dq 118 2 9 9 CD cord-329061-1xut73dq 118 3 ] ] -RRB- cord-329061-1xut73dq 118 4 . . . cord-329061-1xut73dq 119 1 Chandipura Chandipura NNP cord-329061-1xut73dq 119 2 virus virus NN cord-329061-1xut73dq 119 3 was be VBD cord-329061-1xut73dq 119 4 used use VBN cord-329061-1xut73dq 119 5 as as IN cord-329061-1xut73dq 119 6 RNA RNA NNP cord-329061-1xut73dq 119 7 control control NN cord-329061-1xut73dq 119 8 ( ( -LRB- cord-329061-1xut73dq 119 9 P. P. NNP cord-329061-1xut73dq 119 10 N. N. NNP cord-329061-1xut73dq 119 11 Bhatt Bhatt NNP cord-329061-1xut73dq 119 12 , , , cord-329061-1xut73dq 120 1 unpub unpub NNP cord-329061-1xut73dq 120 2 - - HYPH cord-329061-1xut73dq 120 3 Hour Hour NNP cord-329061-1xut73dq 120 4 in in IN cord-329061-1xut73dq 120 5 the the DT cord-329061-1xut73dq 120 6 cerebellum cerebellum NN cord-329061-1xut73dq 120 7 . . . cord-329061-1xut73dq 121 1 Affected affected JJ cord-329061-1xut73dq 121 2 neurons neuron NNS cord-329061-1xut73dq 121 3 were be VBD cord-329061-1xut73dq 121 4 pyknotic pyknotic JJ cord-329061-1xut73dq 121 5 and and CC cord-329061-1xut73dq 121 6 densely densely RB cord-329061-1xut73dq 121 7 eosinophilic eosinophilic JJ cord-329061-1xut73dq 121 8 . . . cord-329061-1xut73dq 122 1 In in IN cord-329061-1xut73dq 122 2 addition addition NN cord-329061-1xut73dq 122 3 , , , cord-329061-1xut73dq 122 4 there there EX cord-329061-1xut73dq 122 5 was be VBD cord-329061-1xut73dq 122 6 a a DT cord-329061-1xut73dq 122 7 scattering scattering NN cord-329061-1xut73dq 122 8 of of IN cord-329061-1xut73dq 122 9 shrunken shrunken JJ cord-329061-1xut73dq 122 10 , , , cord-329061-1xut73dq 122 11 densely densely RB cord-329061-1xut73dq 122 12 staining stain VBG cord-329061-1xut73dq 122 13 astrocytes astrocyte NNS cord-329061-1xut73dq 122 14 in in IN cord-329061-1xut73dq 122 15 these these DT cord-329061-1xut73dq 122 16 areas area NNS cord-329061-1xut73dq 122 17 . . . cord-329061-1xut73dq 123 1 Occasionally occasionally RB cord-329061-1xut73dq 123 2 there there EX cord-329061-1xut73dq 123 3 was be VBD cord-329061-1xut73dq 123 4 hypertrophy hypertrophy NN cord-329061-1xut73dq 123 5 and and CC cord-329061-1xut73dq 123 6 hyperplasia hyperplasia NN cord-329061-1xut73dq 123 7 of of IN cord-329061-1xut73dq 123 8 capillary capillary JJ cord-329061-1xut73dq 123 9 endothelial endothelial JJ cord-329061-1xut73dq 123 10 cells cell NNS cord-329061-1xut73dq 123 11 and and CC cord-329061-1xut73dq 123 12 minimal minimal JJ cord-329061-1xut73dq 123 13 perivascular perivascular JJ cord-329061-1xut73dq 123 14 cuffing cuffing NN cord-329061-1xut73dq 123 15 with with IN cord-329061-1xut73dq 123 16 mononuclear mononuclear JJ cord-329061-1xut73dq 123 17 cells cell NNS cord-329061-1xut73dq 123 18 . . . cord-329061-1xut73dq 124 1 Sometimes sometimes RB cord-329061-1xut73dq 124 2 a a DT cord-329061-1xut73dq 124 3 few few JJ cord-329061-1xut73dq 124 4 polymorphonuclear polymorphonuclear JJ cord-329061-1xut73dq 124 5 leukocytes leukocyte NNS cord-329061-1xut73dq 124 6 were be VBD cord-329061-1xut73dq 124 7 scattered scatter VBN cord-329061-1xut73dq 124 8 in in IN cord-329061-1xut73dq 124 9 areas area NNS cord-329061-1xut73dq 124 10 of of IN cord-329061-1xut73dq 124 11 destruction destruction NN cord-329061-1xut73dq 124 12 . . . cord-329061-1xut73dq 125 1 Spinal spinal JJ cord-329061-1xut73dq 125 2 cord cord NN cord-329061-1xut73dq 125 3 , , , cord-329061-1xut73dq 125 4 salivary salivary JJ cord-329061-1xut73dq 125 5 glands gland NNS cord-329061-1xut73dq 125 6 , , , cord-329061-1xut73dq 125 7 lung lung NN cord-329061-1xut73dq 125 8 , , , cord-329061-1xut73dq 125 9 heart heart NN cord-329061-1xut73dq 125 10 , , , cord-329061-1xut73dq 125 11 liver liver NN cord-329061-1xut73dq 125 12 , , , cord-329061-1xut73dq 125 13 kidney kidney NN cord-329061-1xut73dq 125 14 , , , cord-329061-1xut73dq 125 15 spleen spleen NNS cord-329061-1xut73dq 125 16 , , , cord-329061-1xut73dq 125 17 and and CC cord-329061-1xut73dq 125 18 intestine intestine NN cord-329061-1xut73dq 125 19 were be VBD cord-329061-1xut73dq 125 20 histologically histologically RB cord-329061-1xut73dq 125 21 normal normal JJ cord-329061-1xut73dq 125 22 . . . cord-329061-1xut73dq 126 1 Immunofluorescence immunofluorescence NN cord-329061-1xut73dq 126 2 procedures procedure NNS cord-329061-1xut73dq 126 3 detected detect VBD cord-329061-1xut73dq 126 4 viral viral JJ cord-329061-1xut73dq 126 5 antigen antigen NN cord-329061-1xut73dq 126 6 in in IN cord-329061-1xut73dq 126 7 regions region NNS cord-329061-1xut73dq 126 8 where where WRB cord-329061-1xut73dq 126 9 frank frank JJ cord-329061-1xut73dq 126 10 cellular cellular JJ cord-329061-1xut73dq 126 11 necrosis necrosis NN cord-329061-1xut73dq 126 12 was be VBD cord-329061-1xut73dq 126 13 seen see VBN cord-329061-1xut73dq 126 14 by by IN cord-329061-1xut73dq 126 15 standard standard JJ cord-329061-1xut73dq 126 16 histologic histologic JJ cord-329061-1xut73dq 126 17 techniques technique NNS cord-329061-1xut73dq 126 18 . . . cord-329061-1xut73dq 127 1 In in IN cord-329061-1xut73dq 127 2 addition addition NN cord-329061-1xut73dq 127 3 , , , cord-329061-1xut73dq 127 4 intense intense JJ cord-329061-1xut73dq 127 5 staining staining NN cord-329061-1xut73dq 127 6 was be VBD cord-329061-1xut73dq 127 7 observed observe VBN cord-329061-1xut73dq 127 8 in in IN cord-329061-1xut73dq 127 9 neuronal neuronal JJ cord-329061-1xut73dq 127 10 cytoplasm cytoplasm NN cord-329061-1xut73dq 127 11 of of IN cord-329061-1xut73dq 127 12 scattered scatter VBN cord-329061-1xut73dq 127 13 cells cell NNS cord-329061-1xut73dq 127 14 that that WDT cord-329061-1xut73dq 127 15 were be VBD cord-329061-1xut73dq 127 16 intact intact JJ cord-329061-1xut73dq 127 17 and and CC cord-329061-1xut73dq 127 18 not not RB cord-329061-1xut73dq 127 19 associated associate VBN cord-329061-1xut73dq 127 20 with with IN cord-329061-1xut73dq 127 21 frank frank JJ cord-329061-1xut73dq 127 22 necrosis necrosis NN cord-329061-1xut73dq 127 23 . . . cord-329061-1xut73dq 128 1 Serial serial JJ cord-329061-1xut73dq 128 2 coronal coronal JJ cord-329061-1xut73dq 128 3 sections section NNS cord-329061-1xut73dq 128 4 had have VBD cord-329061-1xut73dq 128 5 immunofluorescence immunofluorescence NN cord-329061-1xut73dq 128 6 staining staining NN cord-329061-1xut73dq 128 7 in in IN cord-329061-1xut73dq 128 8 dorsal dorsal JJ cord-329061-1xut73dq 128 9 cortical cortical JJ cord-329061-1xut73dq 128 10 areas area NNS cord-329061-1xut73dq 128 11 , , , cord-329061-1xut73dq 128 12 the the DT cord-329061-1xut73dq 128 13 ventral ventral JJ cord-329061-1xut73dq 128 14 portion portion NN cord-329061-1xut73dq 128 15 of of IN cord-329061-1xut73dq 128 16 Ammon Ammon NNP cord-329061-1xut73dq 128 17 's 's POS cord-329061-1xut73dq 128 18 horn horn NN cord-329061-1xut73dq 128 19 , , , cord-329061-1xut73dq 128 20 the the DT cord-329061-1xut73dq 128 21 hypothalamus hypothalamus NN cord-329061-1xut73dq 128 22 , , , cord-329061-1xut73dq 128 23 and and CC cord-329061-1xut73dq 128 24 the the DT cord-329061-1xut73dq 128 25 brain brain NN cord-329061-1xut73dq 128 26 stem stem NN cord-329061-1xut73dq 128 27 , , , cord-329061-1xut73dq 128 28 but but CC cord-329061-1xut73dq 128 29 fluorescence fluorescence NN cord-329061-1xut73dq 128 30 was be VBD cord-329061-1xut73dq 128 31 rarely rarely RB cord-329061-1xut73dq 128 32 found find VBN cord-329061-1xut73dq 128 33 in in IN cord-329061-1xut73dq 128 34 cerebellar cerebellar NNP cord-329061-1xut73dq 128 35 folia folia NNP cord-329061-1xut73dq 128 36 and and CC cord-329061-1xut73dq 128 37 white white JJ cord-329061-1xut73dq 128 38 matter matter NN cord-329061-1xut73dq 128 39 . . . cord-329061-1xut73dq 129 1 A a DT cord-329061-1xut73dq 129 2 ttempts ttempt NNS cord-329061-1xut73dq 129 3 to to TO cord-329061-1xut73dq 129 4 adapt adapt VB cord-329061-1xut73dq 129 5 the the DT cord-329061-1xut73dq 129 6 agent agent NN cord-329061-1xut73dq 129 7 to to TO cord-329061-1xut73dq 129 8 monolayer monolayer VB cord-329061-1xut73dq 129 9 cell cell NN cord-329061-1xut73dq 129 10 cultures culture NNS cord-329061-1xut73dq 129 11 . . . cord-329061-1xut73dq 130 1 The the DT cord-329061-1xut73dq 130 2 original original JJ cord-329061-1xut73dq 130 3 salivary salivary NN cord-329061-1xut73dq 130 4 - - HYPH cord-329061-1xut73dq 130 5 gland gland NN cord-329061-1xut73dq 130 6 suspension suspension NN cord-329061-1xut73dq 130 7 inoculated inoculate VBN cord-329061-1xut73dq 130 8 onto onto IN cord-329061-1xut73dq 130 9 various various JJ cord-329061-1xut73dq 130 10 monolayer monolayer NN cord-329061-1xut73dq 130 11 cell cell NN cord-329061-1xut73dq 130 12 cultures culture NNS cord-329061-1xut73dq 130 13 produced produce VBD cord-329061-1xut73dq 130 14 no no DT cord-329061-1xut73dq 130 15 detectable detectable JJ cord-329061-1xut73dq 130 16 CPE CPE NNP cord-329061-1xut73dq 130 17 up up IN cord-329061-1xut73dq 130 18 to to TO cord-329061-1xut73dq 130 19 21 21 CD cord-329061-1xut73dq 130 20 days day NNS cord-329061-1xut73dq 130 21 after after IN cord-329061-1xut73dq 130 22 inoculation inoculation NN cord-329061-1xut73dq 130 23 . . . cord-329061-1xut73dq 131 1 When when WRB cord-329061-1xut73dq 131 2 blind blind JJ cord-329061-1xut73dq 131 3 passages passage NNS cord-329061-1xut73dq 131 4 were be VBD cord-329061-1xut73dq 131 5 made make VBN cord-329061-1xut73dq 131 6 and and CC cord-329061-1xut73dq 131 7 cultures culture NNS cord-329061-1xut73dq 131 8 were be VBD cord-329061-1xut73dq 131 9 challenged challenge VBN cord-329061-1xut73dq 131 10 with with IN cord-329061-1xut73dq 131 11 Chandipura Chandipura NNP cord-329061-1xut73dq 131 12 virus virus NN cord-329061-1xut73dq 131 13 [ [ -LRB- cord-329061-1xut73dq 131 14 8 8 CD cord-329061-1xut73dq 131 15 ] ] -RRB- cord-329061-1xut73dq 131 16 , , , cord-329061-1xut73dq 131 17 interference interference NN cord-329061-1xut73dq 131 18 was be VBD cord-329061-1xut73dq 131 19 not not RB cord-329061-1xut73dq 131 20 observed observe VBN cord-329061-1xut73dq 131 21 . . . cord-329061-1xut73dq 132 1 Fluid fluid NN cord-329061-1xut73dq 132 2 from from IN cord-329061-1xut73dq 132 3 the the DT cord-329061-1xut73dq 132 4 second second JJ cord-329061-1xut73dq 132 5 passage passage NN cord-329061-1xut73dq 132 6 in in IN cord-329061-1xut73dq 132 7 tissue tissue NN cord-329061-1xut73dq 132 8 culture culture NN cord-329061-1xut73dq 132 9 was be VBD cord-329061-1xut73dq 132 10 inoculated inoculate VBN cord-329061-1xut73dq 132 11 ic ic NNP cord-329061-1xut73dq 132 12 into into IN cord-329061-1xut73dq 132 13 infant infant JJ cord-329061-1xut73dq 132 14 mice mouse NNS cord-329061-1xut73dq 132 15 ; ; : cord-329061-1xut73dq 132 16 the the DT cord-329061-1xut73dq 132 17 results result NNS cord-329061-1xut73dq 132 18 were be VBD cord-329061-1xut73dq 132 19 negative negative JJ cord-329061-1xut73dq 132 20 . . . cord-329061-1xut73dq 133 1 Attempts attempt NNS cord-329061-1xut73dq 133 2 were be VBD cord-329061-1xut73dq 133 3 made make VBN cord-329061-1xut73dq 133 4 to to TO cord-329061-1xut73dq 133 5 propagate propagate VB cord-329061-1xut73dq 133 6 mouse mouse NN cord-329061-1xut73dq 133 7 - - HYPH cord-329061-1xut73dq 133 8 brainadapted brainadapte VBN cord-329061-1xut73dq 133 9 virus virus NN cord-329061-1xut73dq 133 10 to to IN cord-329061-1xut73dq 133 11 cell cell NN cord-329061-1xut73dq 133 12 - - HYPH cord-329061-1xut73dq 133 13 culture culture NN cord-329061-1xut73dq 133 14 systems system NNS cord-329061-1xut73dq 133 15 , , , cord-329061-1xut73dq 133 16 such such JJ cord-329061-1xut73dq 133 17 as as IN cord-329061-1xut73dq 133 18 monolayers monolayer NNS cord-329061-1xut73dq 133 19 obtained obtain VBN cord-329061-1xut73dq 133 20 from from IN cord-329061-1xut73dq 133 21 explant explant JJ cord-329061-1xut73dq 133 22 cultures culture NNS cord-329061-1xut73dq 133 23 of of IN cord-329061-1xut73dq 133 24 parotid parotid NN cord-329061-1xut73dq 133 25 , , , cord-329061-1xut73dq 133 26 Harderian Harderian NNP cord-329061-1xut73dq 133 27 , , , cord-329061-1xut73dq 133 28 exorbital exorbital JJ cord-329061-1xut73dq 133 29 , , , cord-329061-1xut73dq 133 30 and and CC cord-329061-1xut73dq 133 31 submaxillary submaxillary JJ cord-329061-1xut73dq 133 32 glands gland NNS cord-329061-1xut73dq 133 33 of of IN cord-329061-1xut73dq 133 34 germfree germfree JJ cord-329061-1xut73dq 133 35 rats rat NNS cord-329061-1xut73dq 133 36 and and CC cord-329061-1xut73dq 133 37 monolayers monolayer NNS cord-329061-1xut73dq 133 38 of of IN cord-329061-1xut73dq 133 39 trypsindispersed trypsindispersed JJ cord-329061-1xut73dq 133 40 infant infant NN cord-329061-1xut73dq 133 41 - - HYPH cord-329061-1xut73dq 133 42 mouse mouse NN cord-329061-1xut73dq 133 43 - - HYPH cord-329061-1xut73dq 133 44 brain brain NN cord-329061-1xut73dq 133 45 cultures culture NNS cord-329061-1xut73dq 133 46 . . . cord-329061-1xut73dq 134 1 There there EX cord-329061-1xut73dq 134 2 was be VBD cord-329061-1xut73dq 134 3 no no DT cord-329061-1xut73dq 134 4 detectable detectable JJ cord-329061-1xut73dq 134 5 CPE CPE NNP cord-329061-1xut73dq 134 6 . . . cord-329061-1xut73dq 135 1 Similar similar JJ cord-329061-1xut73dq 135 2 results result NNS cord-329061-1xut73dq 135 3 were be VBD cord-329061-1xut73dq 135 4 obtained obtain VBN cord-329061-1xut73dq 135 5 with with IN cord-329061-1xut73dq 135 6 the the DT cord-329061-1xut73dq 135 7 Py Py NNP cord-329061-1xut73dq 135 8 - - HYPH cord-329061-1xut73dq 135 9 AL AL NNP cord-329061-1xut73dq 135 10 / / SYM cord-329061-1xut73dq 135 11 N n CD cord-329061-1xut73dq 135 12 cell cell NN cord-329061-1xut73dq 135 13 line line NN cord-329061-1xut73dq 135 14 . . . cord-329061-1xut73dq 136 1 Infectious infectious JJ cord-329061-1xut73dq 136 2 virus virus NN cord-329061-1xut73dq 136 3 or or CC cord-329061-1xut73dq 136 4 viral viral JJ cord-329061-1xut73dq 136 5 antigen antigen NN cord-329061-1xut73dq 136 6 was be VBD cord-329061-1xut73dq 136 7 not not RB cord-329061-1xut73dq 136 8 detected detect VBN cord-329061-1xut73dq 136 9 when when WRB cord-329061-1xut73dq 136 10 tissue tissue NN cord-329061-1xut73dq 136 11 - - HYPH cord-329061-1xut73dq 136 12 culture culture NN cord-329061-1xut73dq 136 13 fluids fluid NNS cord-329061-1xut73dq 136 14 from from IN cord-329061-1xut73dq 136 15 infected infect VBN cord-329061-1xut73dq 136 16 - - HYPH cord-329061-1xut73dq 136 17 mouse mouse NN cord-329061-1xut73dq 136 18 - - HYPH cord-329061-1xut73dq 136 19 brain brain NN cord-329061-1xut73dq 136 20 and and CC cord-329061-1xut73dq 136 21 Py Py NNP cord-329061-1xut73dq 136 22 - - HYPH cord-329061-1xut73dq 136 23 AL AL NNP cord-329061-1xut73dq 136 24 / / SYM cord-329061-1xut73dq 136 25 N n CD cord-329061-1xut73dq 136 26 cultures culture NNS cord-329061-1xut73dq 136 27 were be VBD cord-329061-1xut73dq 136 28 inoculated inoculate VBN cord-329061-1xut73dq 136 29 ic ic NNP cord-329061-1xut73dq 136 30 into into IN cord-329061-1xut73dq 136 31 infant infant JJ cord-329061-1xut73dq 136 32 mice mouse NNS cord-329061-1xut73dq 136 33 or or CC cord-329061-1xut73dq 136 34 when when WRB cord-329061-1xut73dq 136 35 monolayers monolayer NNS cord-329061-1xut73dq 136 36 were be VBD cord-329061-1xut73dq 136 37 examined examine VBN cord-329061-1xut73dq 136 38 by by IN cord-329061-1xut73dq 136 39 indirect indirect JJ cord-329061-1xut73dq 136 40 immunofluorescence immunofluorescence NN cord-329061-1xut73dq 136 41 . . . cord-329061-1xut73dq 137 1 However however RB cord-329061-1xut73dq 137 2 , , , cord-329061-1xut73dq 137 3 PRK PRK NNP cord-329061-1xut73dq 137 4 cultures culture NNS cord-329061-1xut73dq 137 5 showed show VBD cord-329061-1xut73dq 137 6 CPE CPE NNP cord-329061-1xut73dq 137 7 characterized characterize VBN cord-329061-1xut73dq 137 8 by by IN cord-329061-1xut73dq 137 9 formation formation NN cord-329061-1xut73dq 137 10 of of IN cord-329061-1xut73dq 137 11 multinucleated multinucleated JJ cord-329061-1xut73dq 137 12 giant giant JJ cord-329061-1xut73dq 137 13 cells cell NNS cord-329061-1xut73dq 137 14 , , , cord-329061-1xut73dq 137 15 which which WDT cord-329061-1xut73dq 137 16 were be VBD cord-329061-1xut73dq 137 17 seen see VBN cord-329061-1xut73dq 137 18 as as IN cord-329061-1xut73dq 137 19 highly highly RB cord-329061-1xut73dq 137 20 reflective reflective JJ cord-329061-1xut73dq 137 21 masses masse NNS cord-329061-1xut73dq 137 22 . . . cord-329061-1xut73dq 138 1 These these DT cord-329061-1xut73dq 138 2 cells cell NNS cord-329061-1xut73dq 138 3 fell fall VBD cord-329061-1xut73dq 138 4 off off IN cord-329061-1xut73dq 138 5 the the DT cord-329061-1xut73dq 138 6 glass glass NN cord-329061-1xut73dq 138 7 wall wall NN cord-329061-1xut73dq 138 8 a a DT cord-329061-1xut73dq 138 9 few few JJ cord-329061-1xut73dq 138 10 hours hour NNS cord-329061-1xut73dq 138 11 later later RB cord-329061-1xut73dq 138 12 and and CC cord-329061-1xut73dq 138 13 were be VBD cord-329061-1xut73dq 138 14 seen see VBN cord-329061-1xut73dq 138 15 floating float VBG cord-329061-1xut73dq 138 16 in in IN cord-329061-1xut73dq 138 17 medium medium NN cord-329061-1xut73dq 138 18 . . . cord-329061-1xut73dq 139 1 Tissue tissue NN cord-329061-1xut73dq 139 2 - - HYPH cord-329061-1xut73dq 139 3 culture culture NN cord-329061-1xut73dq 139 4 fluids fluid NNS cord-329061-1xut73dq 139 5 of of IN cord-329061-1xut73dq 139 6 these these DT cord-329061-1xut73dq 139 7 cultures culture NNS cord-329061-1xut73dq 139 8 contained contain VBD cord-329061-1xut73dq 139 9 virus virus NN cord-329061-1xut73dq 139 10 as as IN cord-329061-1xut73dq 139 11 detected detect VBN cord-329061-1xut73dq 139 12 in in IN cord-329061-1xut73dq 139 13 infant infant NN cord-329061-1xut73dq 139 14 mice mouse NNS cord-329061-1xut73dq 139 15 , , , cord-329061-1xut73dq 139 16 and and CC cord-329061-1xut73dq 139 17 cultures culture NNS cord-329061-1xut73dq 139 18 were be VBD cord-329061-1xut73dq 139 19 positive positive JJ cord-329061-1xut73dq 139 20 for for IN cord-329061-1xut73dq 139 21 viral viral JJ cord-329061-1xut73dq 139 22 antigen antigen NN cord-329061-1xut73dq 139 23 by by IN cord-329061-1xut73dq 139 24 indirect indirect JJ cord-329061-1xut73dq 139 25 immunofluorescence immunofluorescence NN cord-329061-1xut73dq 139 26 . . . cord-329061-1xut73dq 140 1 Sensitivity sensitivity NN cord-329061-1xut73dq 140 2 of of IN cord-329061-1xut73dq 140 3 SDA SDA NNP cord-329061-1xut73dq 140 4 virus virus NN cord-329061-1xut73dq 140 5 to to IN cord-329061-1xut73dq 140 6 a a DT cord-329061-1xut73dq 140 7 lipid lipid NN cord-329061-1xut73dq 140 8 solvent solvent NN cord-329061-1xut73dq 140 9 was be VBD cord-329061-1xut73dq 140 10 also also RB cord-329061-1xut73dq 140 11 tested test VBN cord-329061-1xut73dq 140 12 . . . cord-329061-1xut73dq 141 1 The the DT cord-329061-1xut73dq 141 2 titer titer NN cord-329061-1xut73dq 141 3 of of IN cord-329061-1xut73dq 141 4 virus virus NN cord-329061-1xut73dq 141 5 was be VBD cord-329061-1xut73dq 141 6 10 10 CD cord-329061-1xut73dq 141 7 3 3 CD cord-329061-1xut73dq 141 8 . . . cord-329061-1xut73dq 142 1 8 8 CD cord-329061-1xut73dq 142 2 and and CC cord-329061-1xut73dq 142 3 < < XX cord-329061-1xut73dq 142 4 10 10 CD cord-329061-1xut73dq 142 5 2 2 CD cord-329061-1xut73dq 142 6 . . . cord-329061-1xut73dq 142 7 0 0 NFP cord-329061-1xut73dq 143 1 IMLD50/0.015 IMLD50/0.015 NNP cord-329061-1xut73dq 143 2 ml ml NNP cord-329061-1xut73dq 143 3 for for IN cord-329061-1xut73dq 143 4 controls control NNS cord-329061-1xut73dq 143 5 and and CC cord-329061-1xut73dq 143 6 chloroformtreated chloroformtreated JJ cord-329061-1xut73dq 143 7 samples sample NNS cord-329061-1xut73dq 143 8 , , , cord-329061-1xut73dq 143 9 respectively respectively RB cord-329061-1xut73dq 143 10 . . . cord-329061-1xut73dq 144 1 The the DT cord-329061-1xut73dq 144 2 test test NN cord-329061-1xut73dq 144 3 was be VBD cord-329061-1xut73dq 144 4 repeated repeat VBN cord-329061-1xut73dq 144 5 with with IN cord-329061-1xut73dq 144 6 similar similar JJ cord-329061-1xut73dq 144 7 results result NNS cord-329061-1xut73dq 144 8 , , , cord-329061-1xut73dq 144 9 and and CC cord-329061-1xut73dq 144 10 it -PRON- PRP cord-329061-1xut73dq 144 11 was be VBD cord-329061-1xut73dq 144 12 concluded conclude VBN cord-329061-1xut73dq 144 13 that that IN cord-329061-1xut73dq 144 14 the the DT cord-329061-1xut73dq 144 15 agent agent NN cord-329061-1xut73dq 144 16 is be VBZ cord-329061-1xut73dq 144 17 sensitive sensitive JJ cord-329061-1xut73dq 144 18 to to IN cord-329061-1xut73dq 144 19 lipid lipid NN cord-329061-1xut73dq 144 20 solvents solvent NNS cord-329061-1xut73dq 144 21 . . . cord-329061-1xut73dq 145 1 Effect effect NN cord-329061-1xut73dq 145 2 of of IN cord-329061-1xut73dq 145 3 low low JJ cord-329061-1xut73dq 145 4 pH ph NN cord-329061-1xut73dq 145 5 on on IN cord-329061-1xut73dq 145 6 infectivity infectivity NN cord-329061-1xut73dq 145 7 . . . cord-329061-1xut73dq 146 1 The the DT cord-329061-1xut73dq 146 2 test test NN cord-329061-1xut73dq 146 3 was be VBD cord-329061-1xut73dq 146 4 performed perform VBN cord-329061-1xut73dq 146 5 as as IN cord-329061-1xut73dq 146 6 described describe VBN cord-329061-1xut73dq 146 7 by by IN cord-329061-1xut73dq 146 8 Leibhaber Leibhaber NNP cord-329061-1xut73dq 146 9 [ [ -LRB- cord-329061-1xut73dq 146 10 13 13 CD cord-329061-1xut73dq 146 11 ] ] -RRB- cord-329061-1xut73dq 146 12 . . . cord-329061-1xut73dq 147 1 Tenfold tenfold IN cord-329061-1xut73dq 147 2 serial serial JJ cord-329061-1xut73dq 147 3 dilutions dilution NNS cord-329061-1xut73dq 147 4 of of IN cord-329061-1xut73dq 147 5 infected infected JJ cord-329061-1xut73dq 147 6 - - HYPH cord-329061-1xut73dq 147 7 mouse mouse NN cord-329061-1xut73dq 147 8 - - HYPH cord-329061-1xut73dq 147 9 brain brain NN cord-329061-1xut73dq 147 10 suspension suspension NN cord-329061-1xut73dq 147 11 kept keep VBD cord-329061-1xut73dq 147 12 at at IN cord-329061-1xut73dq 147 13 different different JJ cord-329061-1xut73dq 147 14 pH ph NN cord-329061-1xut73dq 147 15 values value NNS cord-329061-1xut73dq 147 16 were be VBD cord-329061-1xut73dq 147 17 made make VBN cord-329061-1xut73dq 147 18 in in IN cord-329061-1xut73dq 147 19 Eagle Eagle NNP cord-329061-1xut73dq 147 20 's 's POS cord-329061-1xut73dq 147 21 minimal minimal JJ cord-329061-1xut73dq 147 22 essential essential JJ cord-329061-1xut73dq 147 23 medium medium NN cord-329061-1xut73dq 147 24 in in IN cord-329061-1xut73dq 147 25 Earle Earle NNP cord-329061-1xut73dq 147 26 's 's POS cord-329061-1xut73dq 147 27 base base NN cord-329061-1xut73dq 147 28 with with IN cord-329061-1xut73dq 147 29 3 3 CD cord-329061-1xut73dq 147 30 % % NN cord-329061-1xut73dq 147 31 fetal fetal JJ cord-329061-1xut73dq 147 32 bovine bovine JJ cord-329061-1xut73dq 147 33 serum serum NN cord-329061-1xut73dq 147 34 ( ( -LRB- cord-329061-1xut73dq 147 35 FBS FBS NNP cord-329061-1xut73dq 147 36 ) ) -RRB- cord-329061-1xut73dq 147 37 . . . cord-329061-1xut73dq 148 1 The the DT cord-329061-1xut73dq 148 2 pH ph NN cord-329061-1xut73dq 148 3 of of IN cord-329061-1xut73dq 148 4 each each DT cord-329061-1xut73dq 148 5 dilution dilution NN cord-329061-1xut73dq 148 6 was be VBD cord-329061-1xut73dq 148 7 adjusted adjust VBN cord-329061-1xut73dq 148 8 to to IN cord-329061-1xut73dq 148 9 approximately approximately RB cord-329061-1xut73dq 148 10 7.0 7.0 CD cord-329061-1xut73dq 148 11 by by IN cord-329061-1xut73dq 148 12 addition addition NN cord-329061-1xut73dq 148 13 of of IN cord-329061-1xut73dq 148 14 Tris Tris NNP cord-329061-1xut73dq 148 15 , , , cord-329061-1xut73dq 148 16 and and CC cord-329061-1xut73dq 148 17 this this DT cord-329061-1xut73dq 148 18 solution solution NN cord-329061-1xut73dq 148 19 was be VBD cord-329061-1xut73dq 148 20 inoculated inoculate VBN cord-329061-1xut73dq 148 21 into into IN cord-329061-1xut73dq 148 22 mice mouse NNS cord-329061-1xut73dq 148 23 . . . cord-329061-1xut73dq 149 1 End end NN cord-329061-1xut73dq 149 2 points point NNS cord-329061-1xut73dq 149 3 of of IN cord-329061-1xut73dq 149 4 infectivity infectivity NN cord-329061-1xut73dq 149 5 were be VBD cord-329061-1xut73dq 149 6 calculated calculate VBN cord-329061-1xut73dq 149 7 by by IN cord-329061-1xut73dq 149 8 the the DT cord-329061-1xut73dq 149 9 method method NN cord-329061-1xut73dq 149 10 of of IN cord-329061-1xut73dq 149 11 Reed Reed NNP cord-329061-1xut73dq 149 12 and and CC cord-329061-1xut73dq 149 13 Muench Muench NNP cord-329061-1xut73dq 149 14 [ [ -LRB- cord-329061-1xut73dq 149 15 12 12 CD cord-329061-1xut73dq 149 16 ] ] -RRB- cord-329061-1xut73dq 149 17 . . . cord-329061-1xut73dq 150 1 The the DT cord-329061-1xut73dq 150 2 titers titer NNS cord-329061-1xut73dq 150 3 of of IN cord-329061-1xut73dq 150 4 infectious infectious JJ cord-329061-1xut73dq 150 5 virus virus NN cord-329061-1xut73dq 150 6 detected detect VBN cord-329061-1xut73dq 150 7 in in IN cord-329061-1xut73dq 150 8 PBS PBS NNP cord-329061-1xut73dq 150 9 after after IN cord-329061-1xut73dq 150 10 incubation incubation NN cord-329061-1xut73dq 150 11 for for IN cord-329061-1xut73dq 150 12 3.0 3.0 CD cord-329061-1xut73dq 150 13 hr hr NN cord-329061-1xut73dq 150 14 at at IN cord-329061-1xut73dq 150 15 25 25 CD cord-329061-1xut73dq 150 16 C C NNP cord-329061-1xut73dq 150 17 was be VBD cord-329061-1xut73dq 150 18 103 103 CD cord-329061-1xut73dq 150 19 . . . cord-329061-1xut73dq 151 1 6 6 CD cord-329061-1xut73dq 151 2 IMLD50/0.015 IMLD50/0.015 NNP cord-329061-1xut73dq 151 3 ml ml NNS cord-329061-1xut73dq 151 4 , , , cord-329061-1xut73dq 151 5 whereas whereas IN cord-329061-1xut73dq 151 6 at at IN cord-329061-1xut73dq 151 7 pH pH NNP cord-329061-1xut73dq 151 8 7.0 7.0 CD cord-329061-1xut73dq 151 9 and and CC cord-329061-1xut73dq 151 10 pH pH NNP cord-329061-1xut73dq 151 11 3.0 3.0 NNP cord-329061-1xut73dq 151 12 it -PRON- PRP cord-329061-1xut73dq 151 13 was be VBD cord-329061-1xut73dq 151 14 10 10 CD cord-329061-1xut73dq 151 15 3 3 CD cord-329061-1xut73dq 151 16 . . . cord-329061-1xut73dq 152 1 9 9 CD cord-329061-1xut73dq 152 2 IMLD50/0.015 IMLD50/0.015 NNP cord-329061-1xut73dq 152 3 m1 m1 NNP cord-329061-1xut73dq 152 4 and and CC cord-329061-1xut73dq 152 5 lQ2·8 lQ2·8 NNP cord-329061-1xut73dq 152 6 1MLD50/ 1mld50/ CD cord-329061-1xut73dq 152 7 0.015 0.015 CD cord-329061-1xut73dq 152 8 ml ml NNS cord-329061-1xut73dq 152 9 , , , cord-329061-1xut73dq 152 10 respectively respectively RB cord-329061-1xut73dq 152 11 . . . cord-329061-1xut73dq 153 1 Thus thus RB cord-329061-1xut73dq 153 2 infectious infectious JJ cord-329061-1xut73dq 153 3 virus virus NN cord-329061-1xut73dq 153 4 was be VBD cord-329061-1xut73dq 153 5 relatively relatively RB cord-329061-1xut73dq 153 6 stable stable JJ cord-329061-1xut73dq 153 7 at at IN cord-329061-1xut73dq 153 8 low low JJ cord-329061-1xut73dq 153 9 pH. ph. NN cord-329061-1xut73dq 153 10 Effect Effect NNP cord-329061-1xut73dq 153 11 of of IN cord-329061-1xut73dq 153 12 temperature temperature NN cord-329061-1xut73dq 153 13 on on IN cord-329061-1xut73dq 153 14 infectivity infectivity NN cord-329061-1xut73dq 153 15 . . . cord-329061-1xut73dq 154 1 The the DT cord-329061-1xut73dq 154 2 effect effect NN cord-329061-1xut73dq 154 3 of of IN cord-329061-1xut73dq 154 4 a a DT cord-329061-1xut73dq 154 5 temperature temperature NN cord-329061-1xut73dq 154 6 of of IN cord-329061-1xut73dq 154 7 37 37 CD cord-329061-1xut73dq 154 8 C c NN cord-329061-1xut73dq 154 9 on on IN cord-329061-1xut73dq 154 10 infectious infectious JJ cord-329061-1xut73dq 154 11 virus virus NN cord-329061-1xut73dq 154 12 was be VBD cord-329061-1xut73dq 154 13 determined determine VBN cord-329061-1xut73dq 154 14 as as IN cord-329061-1xut73dq 154 15 outlined outline VBN cord-329061-1xut73dq 154 16 by by IN cord-329061-1xut73dq 154 17 Bhatt Bhatt NNP cord-329061-1xut73dq 154 18 et et NNP cord-329061-1xut73dq 154 19 al al NNP cord-329061-1xut73dq 154 20 . . . cord-329061-1xut73dq 155 1 [ [ -LRB- cord-329061-1xut73dq 155 2 9 9 CD cord-329061-1xut73dq 155 3 ] ] -RRB- cord-329061-1xut73dq 155 4 . . . cord-329061-1xut73dq 156 1 To to TO cord-329061-1xut73dq 156 2 determine determine VB cord-329061-1xut73dq 156 3 the the DT cord-329061-1xut73dq 156 4 effect effect NN cord-329061-1xut73dq 156 5 of of IN cord-329061-1xut73dq 156 6 a a DT cord-329061-1xut73dq 156 7 temperature temperature NN cord-329061-1xut73dq 156 8 of of IN cord-329061-1xut73dq 156 9 56 56 CD cord-329061-1xut73dq 156 10 C c NN cord-329061-1xut73dq 156 11 , , , cord-329061-1xut73dq 156 12 infectivity infectivity NN cord-329061-1xut73dq 156 13 was be VBD cord-329061-1xut73dq 156 14 determined determine VBN cord-329061-1xut73dq 156 15 at at IN cord-329061-1xut73dq 156 16 intervals interval NNS cord-329061-1xut73dq 156 17 of of IN cord-329061-1xut73dq 156 18 0 0 CD cord-329061-1xut73dq 156 19 , , , cord-329061-1xut73dq 156 20 5 5 CD cord-329061-1xut73dq 156 21 , , , cord-329061-1xut73dq 156 22 and and CC cord-329061-1xut73dq 156 23 10 10 CD cord-329061-1xut73dq 156 24 min min NN cord-329061-1xut73dq 156 25 . . . cord-329061-1xut73dq 157 1 An an DT cord-329061-1xut73dq 157 2 aliquot aliquot NN cord-329061-1xut73dq 157 3 of of IN cord-329061-1xut73dq 157 4 viral viral JJ cord-329061-1xut73dq 157 5 stock stock NN cord-329061-1xut73dq 157 6 was be VBD cord-329061-1xut73dq 157 7 kept keep VBN cord-329061-1xut73dq 157 8 at at IN cord-329061-1xut73dq 157 9 4 4 CD cord-329061-1xut73dq 157 10 C c NN cord-329061-1xut73dq 157 11 , , , cord-329061-1xut73dq 157 12 and and CC cord-329061-1xut73dq 157 13 infectivity infectivity NN cord-329061-1xut73dq 157 14 was be VBD cord-329061-1xut73dq 157 15 determined determine VBN cord-329061-1xut73dq 157 16 on on IN cord-329061-1xut73dq 157 17 days day NNS cord-329061-1xut73dq 157 18 0 0 CD cord-329061-1xut73dq 157 19 , , , cord-329061-1xut73dq 157 20 7 7 CD cord-329061-1xut73dq 157 21 , , , cord-329061-1xut73dq 157 22 and and CC cord-329061-1xut73dq 157 23 28 28 CD cord-329061-1xut73dq 157 24 . . . cord-329061-1xut73dq 158 1 Infectivity infectivity NN cord-329061-1xut73dq 158 2 of of IN cord-329061-1xut73dq 158 3 viral viral JJ cord-329061-1xut73dq 158 4 strain strain NN cord-329061-1xut73dq 158 5 681 681 CD cord-329061-1xut73dq 158 6 was be VBD cord-329061-1xut73dq 158 7 stable stable JJ cord-329061-1xut73dq 158 8 in in IN cord-329061-1xut73dq 158 9 PBS PBS NNP cord-329061-1xut73dq 158 10 plus plus CC cord-329061-1xut73dq 158 11 3 3 CD cord-329061-1xut73dq 158 12 % % NN cord-329061-1xut73dq 158 13 FBS FBS NNP cord-329061-1xut73dq 158 14 at at IN cord-329061-1xut73dq 158 15 37 37 CD cord-329061-1xut73dq 158 16 C c NN cord-329061-1xut73dq 158 17 for for IN cord-329061-1xut73dq 158 18 3 3 CD cord-329061-1xut73dq 158 19 hr hr NN cord-329061-1xut73dq 158 20 ; ; : cord-329061-1xut73dq 158 21 the the DT cord-329061-1xut73dq 158 22 titer titer NN cord-329061-1xut73dq 158 23 then then RB cord-329061-1xut73dq 158 24 decreased decrease VBD cord-329061-1xut73dq 158 25 by by IN cord-329061-1xut73dq 158 26 1.1 1.1 CD cord-329061-1xut73dq 158 27 log log NN cord-329061-1xut73dq 158 28 . . . cord-329061-1xut73dq 159 1 , , , cord-329061-1xut73dq 159 2 by by IN cord-329061-1xut73dq 159 3 5 5 CD cord-329061-1xut73dq 159 4 hr hr NN cord-329061-1xut73dq 159 5 . . . cord-329061-1xut73dq 160 1 At at IN cord-329061-1xut73dq 160 2 56 56 CD cord-329061-1xut73dq 160 3 C c NN cord-329061-1xut73dq 160 4 the the DT cord-329061-1xut73dq 160 5 infectivity infectivity NN cord-329061-1xut73dq 160 6 decreased decrease VBD cord-329061-1xut73dq 160 7 from from IN cord-329061-1xut73dq 160 8 10 10 CD cord-329061-1xut73dq 160 9 3 3 CD cord-329061-1xut73dq 160 10 . . . cord-329061-1xut73dq 160 11 8 8 CD cord-329061-1xut73dq 160 12 1MLD50 1mld50 CD cord-329061-1xut73dq 160 13 at at IN cord-329061-1xut73dq 160 14 zero zero CD cord-329061-1xut73dq 160 15 time time NN cord-329061-1xut73dq 160 16 to to TO cord-329061-1xut73dq 160 17 trace trace NN cord-329061-1xut73dq 160 18 levels level NNS cord-329061-1xut73dq 160 19 by by IN cord-329061-1xut73dq 160 20 5 5 CD cord-329061-1xut73dq 161 1 The the DT cord-329061-1xut73dq 161 2 size size NN cord-329061-1xut73dq 161 3 of of IN cord-329061-1xut73dq 161 4 infectious infectious JJ cord-329061-1xut73dq 161 5 viral viral JJ cord-329061-1xut73dq 161 6 particles particle NNS cord-329061-1xut73dq 161 7 . . . cord-329061-1xut73dq 162 1 The the DT cord-329061-1xut73dq 162 2 approximate approximate JJ cord-329061-1xut73dq 162 3 size size NN cord-329061-1xut73dq 162 4 of of IN cord-329061-1xut73dq 162 5 infectious infectious JJ cord-329061-1xut73dq 162 6 viral viral JJ cord-329061-1xut73dq 162 7 particles particle NNS cord-329061-1xut73dq 162 8 was be VBD cord-329061-1xut73dq 162 9 determined determine VBN cord-329061-1xut73dq 162 10 by by IN cord-329061-1xut73dq 162 11 the the DT cord-329061-1xut73dq 162 12 method method NN cord-329061-1xut73dq 162 13 of of IN cord-329061-1xut73dq 162 14 Atoynatan Atoynatan NNP cord-329061-1xut73dq 162 15 and and CC cord-329061-1xut73dq 162 16 Hsiung Hsiung NNP cord-329061-1xut73dq 162 17 [ [ -LRB- cord-329061-1xut73dq 162 18 14 14 CD cord-329061-1xut73dq 162 19 ] ] -RRB- cord-329061-1xut73dq 162 20 and and CC cord-329061-1xut73dq 162 21 Casals Casals NNPS cord-329061-1xut73dq 162 22 [ [ -LRB- cord-329061-1xut73dq 162 23 15 15 CD cord-329061-1xut73dq 162 24 ] ] -RRB- cord-329061-1xut73dq 162 25 as as IN cord-329061-1xut73dq 162 26 modified modify VBN cord-329061-1xut73dq 162 27 by by IN cord-329061-1xut73dq 162 28 Bhatt Bhatt NNP cord-329061-1xut73dq 162 29 et et NNP cord-329061-1xut73dq 162 30 al al NNP cord-329061-1xut73dq 162 31 . . . cord-329061-1xut73dq 163 1 [ [ -LRB- cord-329061-1xut73dq 163 2 9 9 CD cord-329061-1xut73dq 163 3 ] ] -RRB- cord-329061-1xut73dq 163 4 . . . cord-329061-1xut73dq 164 1 A a DT cord-329061-1xut73dq 164 2 fresh fresh JJ cord-329061-1xut73dq 164 3 , , , cord-329061-1xut73dq 164 4 10 10 CD cord-329061-1xut73dq 164 5 % % NN cord-329061-1xut73dq 164 6 suspension suspension NN cord-329061-1xut73dq 164 7 of of IN cord-329061-1xut73dq 164 8 mouse mouse NN cord-329061-1xut73dq 164 9 brain brain NN cord-329061-1xut73dq 164 10 was be VBD cord-329061-1xut73dq 164 11 made make VBN cord-329061-1xut73dq 164 12 in in IN cord-329061-1xut73dq 164 13 PBS PBS NNP cord-329061-1xut73dq 164 14 plus plus CC cord-329061-1xut73dq 164 15 FBS FBS NNP cord-329061-1xut73dq 164 16 , , , cord-329061-1xut73dq 164 17 clarified clarify VBN cord-329061-1xut73dq 164 18 by by IN cord-329061-1xut73dq 164 19 centrifugation centrifugation NN cord-329061-1xut73dq 164 20 at at IN cord-329061-1xut73dq 164 21 1,000 1,000 CD cord-329061-1xut73dq 164 22 g g NN cord-329061-1xut73dq 164 23 for for IN cord-329061-1xut73dq 164 24 20 20 CD cord-329061-1xut73dq 164 25 min min NN cord-329061-1xut73dq 164 26 , , , cord-329061-1xut73dq 164 27 and and CC cord-329061-1xut73dq 164 28 filtered filter VBN cord-329061-1xut73dq 164 29 through through IN cord-329061-1xut73dq 164 30 MilIipore MilIipore NNP cord-329061-1xut73dq 164 31 filters filter NNS cord-329061-1xut73dq 164 32 ( ( -LRB- cord-329061-1xut73dq 164 33 Millipore Millipore NNP cord-329061-1xut73dq 164 34 Corp. Corp. NNP cord-329061-1xut73dq 164 35 , , , cord-329061-1xut73dq 164 36 Bedford Bedford NNP cord-329061-1xut73dq 164 37 , , , cord-329061-1xut73dq 164 38 Mass. Massachusetts NNP cord-329061-1xut73dq 164 39 ) ) -RRB- cord-329061-1xut73dq 164 40 of of IN cord-329061-1xut73dq 164 41 various various JJ cord-329061-1xut73dq 164 42 pore pore NN cord-329061-1xut73dq 164 43 sizes size NNS cord-329061-1xut73dq 164 44 . . . cord-329061-1xut73dq 165 1 Viral viral JJ cord-329061-1xut73dq 165 2 titers titer NNS cord-329061-1xut73dq 165 3 obtained obtain VBD cord-329061-1xut73dq 165 4 were be VBD cord-329061-1xut73dq 165 5 103 103 CD cord-329061-1xut73dq 165 6 . . . cord-329061-1xut73dq 166 1 5 5 CD cord-329061-1xut73dq 166 2 , , , cord-329061-1xut73dq 166 3 104.2 104.2 CD cord-329061-1xut73dq 166 4 , , , cord-329061-1xut73dq 166 5 103 103 CD cord-329061-1xut73dq 166 6 . . . cord-329061-1xut73dq 167 1 8 8 CD cord-329061-1xut73dq 167 2 , , , cord-329061-1xut73dq 167 3 10 10 CD cord-329061-1xut73dq 167 4 3 3 CD cord-329061-1xut73dq 167 5 . . . cord-329061-1xut73dq 167 6 0 0 NFP cord-329061-1xut73dq 167 7 , , , cord-329061-1xut73dq 167 8 and and CC cord-329061-1xut73dq 167 9 < < XX cord-329061-1xut73dq 167 10 lQ2 lq2 XX cord-329061-1xut73dq 167 11 1MLD50/0.015 1mld50/0.015 CD cord-329061-1xut73dq 167 12 m1 m1 NNP cord-329061-1xut73dq 167 13 for for IN cord-329061-1xut73dq 167 14 unfiltered unfiltered JJ cord-329061-1xut73dq 167 15 virus virus NN cord-329061-1xut73dq 167 16 and and CC cord-329061-1xut73dq 167 17 after after IN cord-329061-1xut73dq 167 18 filtration filtration NN cord-329061-1xut73dq 167 19 through through IN cord-329061-1xut73dq 167 20 pore pore NN cord-329061-1xut73dq 167 21 sizes size NNS cord-329061-1xut73dq 167 22 1,200 1,200 CD cord-329061-1xut73dq 167 23 urn urn NN cord-329061-1xut73dq 167 24 , , , cord-329061-1xut73dq 167 25 450 450 CD cord-329061-1xut73dq 167 26 / / SYM cord-329061-1xut73dq 167 27 lm lm NNP cord-329061-1xut73dq 167 28 , , , cord-329061-1xut73dq 167 29 220 220 CD cord-329061-1xut73dq 167 30 / / SYM cord-329061-1xut73dq 167 31 lm lm NNP cord-329061-1xut73dq 167 32 , , , cord-329061-1xut73dq 167 33 and and CC cord-329061-1xut73dq 167 34 100 100 CD cord-329061-1xut73dq 167 35 urn urn NN cord-329061-1xut73dq 167 36 , , , cord-329061-1xut73dq 167 37 respectively respectively RB cord-329061-1xut73dq 167 38 . . . cord-329061-1xut73dq 168 1 Results result NNS cord-329061-1xut73dq 168 2 indicate indicate VBP cord-329061-1xut73dq 168 3 that that IN cord-329061-1xut73dq 168 4 the the DT cord-329061-1xut73dq 168 5 size size NN cord-329061-1xut73dq 168 6 of of IN cord-329061-1xut73dq 168 7 the the DT cord-329061-1xut73dq 168 8 virus virus NN cord-329061-1xut73dq 168 9 is be VBZ cord-329061-1xut73dq 168 10 less less JJR cord-329061-1xut73dq 168 11 than than IN cord-329061-1xut73dq 168 12 220 220 CD cord-329061-1xut73dq 168 13 urn urn NN cord-329061-1xut73dq 168 14 but but CC cord-329061-1xut73dq 168 15 greater great JJR cord-329061-1xut73dq 168 16 than than IN cord-329061-1xut73dq 168 17 100 100 CD cord-329061-1xut73dq 168 18 urn urn NN cord-329061-1xut73dq 168 19 . . . cord-329061-1xut73dq 169 1 The the DT cord-329061-1xut73dq 169 2 particles particle NNS cord-329061-1xut73dq 169 3 without without IN cord-329061-1xut73dq 169 4 membranes membrane NNS cord-329061-1xut73dq 169 5 measured measure VBN cord-329061-1xut73dq 169 6 by by IN cord-329061-1xut73dq 169 7 electron electron NN cord-329061-1xut73dq 169 8 microscopy microscopy NNS cord-329061-1xut73dq 169 9 were be VBD cord-329061-1xut73dq 169 10 previously previously RB cord-329061-1xut73dq 169 11 reported report VBN cord-329061-1xut73dq 169 12 to to TO cord-329061-1xut73dq 169 13 be be VB cord-329061-1xut73dq 169 14 6 6 CD cord-329061-1xut73dq 169 15 < < XX cord-329061-1xut73dq 169 16 f--70 f--70 NN cord-329061-1xut73dq 170 1 /lm /lm NFP cord-329061-1xut73dq 170 2 [ [ -LRB- cord-329061-1xut73dq 170 3 1 1 CD cord-329061-1xut73dq 170 4 ] ] -RRB- cord-329061-1xut73dq 170 5 . . . cord-329061-1xut73dq 171 1 Detection detection NN cord-329061-1xut73dq 171 2 of of IN cord-329061-1xut73dq 171 3 hemagglutination hemagglutination NN cord-329061-1xut73dq 171 4 . . . cord-329061-1xut73dq 172 1 Two two CD cord-329061-1xut73dq 172 2 sources source NNS cord-329061-1xut73dq 172 3 of of IN cord-329061-1xut73dq 172 4 antigen antigen NN cord-329061-1xut73dq 172 5 were be VBD cord-329061-1xut73dq 172 6 used use VBN cord-329061-1xut73dq 172 7 . . . cord-329061-1xut73dq 173 1 ( ( -LRB- cord-329061-1xut73dq 173 2 1 1 CD cord-329061-1xut73dq 173 3 ) ) -RRB- cord-329061-1xut73dq 174 1 A a DT cord-329061-1xut73dq 174 2 10 10 CD cord-329061-1xut73dq 174 3 % % NN cord-329061-1xut73dq 174 4 suspension suspension NN cord-329061-1xut73dq 174 5 of of IN cord-329061-1xut73dq 174 6 infected infected JJ cord-329061-1xut73dq 174 7 salivary salivary NN cord-329061-1xut73dq 174 8 gland gland NN cord-329061-1xut73dq 174 9 was be VBD cord-329061-1xut73dq 174 10 tested test VBN cord-329061-1xut73dq 174 11 for for IN cord-329061-1xut73dq 174 12 its -PRON- PRP$ cord-329061-1xut73dq 174 13 capacity capacity NN cord-329061-1xut73dq 174 14 to to TO cord-329061-1xut73dq 174 15 hemagglutinate hemagglutinate VB cord-329061-1xut73dq 174 16 red red JJ cord-329061-1xut73dq 174 17 blood blood NN cord-329061-1xut73dq 174 18 cells cell NNS cord-329061-1xut73dq 174 19 ( ( -LRB- cord-329061-1xut73dq 174 20 RBCs rbc NNS cord-329061-1xut73dq 174 21 ) ) -RRB- cord-329061-1xut73dq 174 22 of of IN cord-329061-1xut73dq 174 23 rabbits rabbit NNS cord-329061-1xut73dq 174 24 , , , cord-329061-1xut73dq 174 25 guinea guinea NN cord-329061-1xut73dq 174 26 pigs pig NNS cord-329061-1xut73dq 174 27 , , , cord-329061-1xut73dq 174 28 and and CC cord-329061-1xut73dq 174 29 geese goose NNS cord-329061-1xut73dq 174 30 by by IN cord-329061-1xut73dq 174 31 the the DT cord-329061-1xut73dq 174 32 method method NN cord-329061-1xut73dq 174 33 of of IN cord-329061-1xut73dq 174 34 Ashe Ashe NNP cord-329061-1xut73dq 174 35 [ [ -LRB- cord-329061-1xut73dq 174 36 16 16 CD cord-329061-1xut73dq 174 37 ] ] -RRB- cord-329061-1xut73dq 174 38 . . . cord-329061-1xut73dq 175 1 ( ( -LRB- cord-329061-1xut73dq 175 2 2 2 LS cord-329061-1xut73dq 175 3 ) ) -RRB- cord-329061-1xut73dq 175 4 Either either CC cord-329061-1xut73dq 175 5 a a DT cord-329061-1xut73dq 175 6 10 10 CD cord-329061-1xut73dq 175 7 % % NN cord-329061-1xut73dq 175 8 suspension suspension NN cord-329061-1xut73dq 175 9 of of IN cord-329061-1xut73dq 175 10 infected infected JJ cord-329061-1xut73dq 175 11 mouse mouse NN cord-329061-1xut73dq 175 12 brain brain NN cord-329061-1xut73dq 175 13 in in IN cord-329061-1xut73dq 175 14 PBS PBS NNP cord-329061-1xut73dq 175 15 or or CC cord-329061-1xut73dq 175 16 infected infected JJ cord-329061-1xut73dq 175 17 mouse mouse NN cord-329061-1xut73dq 175 18 brain brain NN cord-329061-1xut73dq 175 19 extracted extract VBN cord-329061-1xut73dq 175 20 with with IN cord-329061-1xut73dq 175 21 sucrose sucrose NN cord-329061-1xut73dq 175 22 and and CC cord-329061-1xut73dq 175 23 acetone acetone NN cord-329061-1xut73dq 175 24 was be VBD cord-329061-1xut73dq 175 25 tested test VBN cord-329061-1xut73dq 175 26 for for IN cord-329061-1xut73dq 175 27 hem hem NNP cord-329061-1xut73dq 175 28 agglutinability agglutinability NNP cord-329061-1xut73dq 175 29 with with IN cord-329061-1xut73dq 175 30 RBCs rbc NNS cord-329061-1xut73dq 175 31 of of IN cord-329061-1xut73dq 175 32 rats rat NNS cord-329061-1xut73dq 175 33 , , , cord-329061-1xut73dq 175 34 mice mouse NNS cord-329061-1xut73dq 175 35 , , , cord-329061-1xut73dq 175 36 guinea guinea NN cord-329061-1xut73dq 175 37 pigs pig NNS cord-329061-1xut73dq 175 38 , , , cord-329061-1xut73dq 175 39 and and CC cord-329061-1xut73dq 175 40 geese goose NNS cord-329061-1xut73dq 175 41 . . . cord-329061-1xut73dq 176 1 The the DT cord-329061-1xut73dq 176 2 microtiter microtiter NN cord-329061-1xut73dq 176 3 method method NN cord-329061-1xut73dq 176 4 [ [ -LRB- cord-329061-1xut73dq 176 5 11 11 CD cord-329061-1xut73dq 176 6 ] ] -RRB- cord-329061-1xut73dq 176 7 was be VBD cord-329061-1xut73dq 176 8 used use VBN cord-329061-1xut73dq 176 9 for for IN cord-329061-1xut73dq 176 10 all all DT cord-329061-1xut73dq 176 11 tests test NNS cord-329061-1xut73dq 176 12 , , , cord-329061-1xut73dq 176 13 and and CC cord-329061-1xut73dq 176 14 incubation incubation NN cord-329061-1xut73dq 176 15 was be VBD cord-329061-1xut73dq 176 16 at at IN cord-329061-1xut73dq 176 17 4 4 CD cord-329061-1xut73dq 176 18 C C NNP cord-329061-1xut73dq 176 19 , , , cord-329061-1xut73dq 176 20 25 25 CD cord-329061-1xut73dq 176 21 C c NN cord-329061-1xut73dq 176 22 , , , cord-329061-1xut73dq 176 23 and and CC cord-329061-1xut73dq 176 24 37 37 CD cord-329061-1xut73dq 176 25 C. C. NNP cord-329061-1xut73dq 176 26 RBCs rbc NNS cord-329061-1xut73dq 176 27 were be VBD cord-329061-1xut73dq 176 28 suspended suspend VBN cord-329061-1xut73dq 176 29 ( ( -LRB- cord-329061-1xut73dq 176 30 0.5 0.5 CD cord-329061-1xut73dq 176 31 % % NN cord-329061-1xut73dq 176 32 ) ) -RRB- cord-329061-1xut73dq 176 33 in in IN cord-329061-1xut73dq 176 34 PBS PBS NNP cord-329061-1xut73dq 176 35 . . . cord-329061-1xut73dq 177 1 Hemagglutination Hemagglutination NNP cord-329061-1xut73dq 177 2 was be VBD cord-329061-1xut73dq 177 3 not not RB cord-329061-1xut73dq 177 4 detected detect VBN cord-329061-1xut73dq 177 5 at at IN cord-329061-1xut73dq 177 6 4 4 CD cord-329061-1xut73dq 177 7 C C NNP cord-329061-1xut73dq 177 8 , , , cord-329061-1xut73dq 177 9 25 25 CD cord-329061-1xut73dq 177 10 C c NN cord-329061-1xut73dq 177 11 , , , cord-329061-1xut73dq 177 12 or or CC cord-329061-1xut73dq 177 13 37 37 CD cord-329061-1xut73dq 177 14 C c NN cord-329061-1xut73dq 177 15 using use VBG cord-329061-1xut73dq 177 16 a a DT cord-329061-1xut73dq 177 17 suspension suspension NN cord-329061-1xut73dq 177 18 of of IN cord-329061-1xut73dq 177 19 infected infected JJ cord-329061-1xut73dq 177 20 salivary salivary JJ cord-329061-1xut73dq 177 21 gland gland NN cord-329061-1xut73dq 177 22 and and CC cord-329061-1xut73dq 177 23 rabbit rabbit NN cord-329061-1xut73dq 177 24 , , , cord-329061-1xut73dq 177 25 guinea guinea NN cord-329061-1xut73dq 177 26 pig pig NN cord-329061-1xut73dq 177 27 , , , cord-329061-1xut73dq 177 28 or or CC cord-329061-1xut73dq 177 29 goose goose NN cord-329061-1xut73dq 177 30 cells cell NNS cord-329061-1xut73dq 177 31 . . . cord-329061-1xut73dq 178 1 A a DT cord-329061-1xut73dq 178 2 10 10 CD cord-329061-1xut73dq 178 3 % % NN cord-329061-1xut73dq 178 4 suspension suspension NN cord-329061-1xut73dq 178 5 of of IN cord-329061-1xut73dq 178 6 infected infected JJ cord-329061-1xut73dq 178 7 brain brain NN cord-329061-1xut73dq 178 8 in in IN cord-329061-1xut73dq 178 9 PBS PBS NNP cord-329061-1xut73dq 178 10 or or CC cord-329061-1xut73dq 178 11 sucrose sucrose NN cord-329061-1xut73dq 178 12 - - HYPH cord-329061-1xut73dq 178 13 acetone acetone NN cord-329061-1xut73dq 178 14 - - HYPH cord-329061-1xut73dq 178 15 extracted extract VBN cord-329061-1xut73dq 178 16 antigen antigen NN cord-329061-1xut73dq 178 17 from from IN cord-329061-1xut73dq 178 18 mouse mouse NN cord-329061-1xut73dq 178 19 brain brain NN cord-329061-1xut73dq 178 20 gave give VBD cord-329061-1xut73dq 178 21 unsatisfactory unsatisfactory JJ cord-329061-1xut73dq 178 22 results result NNS cord-329061-1xut73dq 178 23 with with IN cord-329061-1xut73dq 178 24 RBCs rbc NNS cord-329061-1xut73dq 178 25 of of IN cord-329061-1xut73dq 178 26 rats rat NNS cord-329061-1xut73dq 178 27 , , , cord-329061-1xut73dq 178 28 guinea guinea NN cord-329061-1xut73dq 178 29 pigs pig NNS cord-329061-1xut73dq 178 30 , , , cord-329061-1xut73dq 178 31 and and CC cord-329061-1xut73dq 178 32 mice mouse NNS cord-329061-1xut73dq 178 33 at at IN cord-329061-1xut73dq 178 34 25 25 CD cord-329061-1xut73dq 178 35 C c NN cord-329061-1xut73dq 178 36 and and CC cord-329061-1xut73dq 178 37 37 37 CD cord-329061-1xut73dq 178 38 C c NN cord-329061-1xut73dq 178 39 , , , cord-329061-1xut73dq 178 40 and and CC cord-329061-1xut73dq 178 41 there there EX cord-329061-1xut73dq 178 42 was be VBD cord-329061-1xut73dq 178 43 no no DT cord-329061-1xut73dq 178 44 HA HA NNP cord-329061-1xut73dq 178 45 activity activity NN cord-329061-1xut73dq 178 46 after after IN cord-329061-1xut73dq 178 47 incubation incubation NN cord-329061-1xut73dq 178 48 overnight overnight RB cord-329061-1xut73dq 178 49 at at IN cord-329061-1xut73dq 178 50 4 4 CD cord-329061-1xut73dq 178 51 C. c. NN cord-329061-1xut73dq 179 1 Neither neither DT cord-329061-1xut73dq 179 2 was be VBD cord-329061-1xut73dq 179 3 HA HA NNP cord-329061-1xut73dq 179 4 detected detect VBD cord-329061-1xut73dq 179 5 for for IN cord-329061-1xut73dq 179 6 goose goose NN cord-329061-1xut73dq 179 7 RBCs rbc NNS cord-329061-1xut73dq 179 8 at at IN cord-329061-1xut73dq 179 9 4 4 CD cord-329061-1xut73dq 179 10 C C NNP cord-329061-1xut73dq 179 11 , , , cord-329061-1xut73dq 179 12 25 25 CD cord-329061-1xut73dq 179 13 C c NN cord-329061-1xut73dq 179 14 , , , cord-329061-1xut73dq 179 15 or or CC cord-329061-1xut73dq 179 16 37 37 CD cord-329061-1xut73dq 179 17 C. C. NNP cord-329061-1xut73dq 179 18 Acridine Acridine NNP cord-329061-1xut73dq 179 19 - - HYPH cord-329061-1xut73dq 179 20 orange orange NN cord-329061-1xut73dq 179 21 staining staining NN cord-329061-1xut73dq 179 22 . . . cord-329061-1xut73dq 180 1 The the DT cord-329061-1xut73dq 180 2 cytoplasm cytoplasm NN cord-329061-1xut73dq 180 3 of of IN cord-329061-1xut73dq 180 4 infected infected JJ cord-329061-1xut73dq 180 5 cells cell NNS cord-329061-1xut73dq 180 6 stained stain VBN cord-329061-1xut73dq 180 7 orange orange JJ cord-329061-1xut73dq 180 8 - - HYPH cord-329061-1xut73dq 180 9 red red NN cord-329061-1xut73dq 180 10 with with IN cord-329061-1xut73dq 180 11 acridine acridine NNP cord-329061-1xut73dq 180 12 orange orange NNP cord-329061-1xut73dq 180 13 , , , cord-329061-1xut73dq 180 14 indicating indicate VBG cord-329061-1xut73dq 180 15 that that IN cord-329061-1xut73dq 180 16 virus virus NN cord-329061-1xut73dq 180 17 replicates replicate VBZ cord-329061-1xut73dq 180 18 in in IN cord-329061-1xut73dq 180 19 the the DT cord-329061-1xut73dq 180 20 cytoplasm cytoplasm NN cord-329061-1xut73dq 180 21 . . . cord-329061-1xut73dq 181 1 Antigenic antigenic JJ cord-329061-1xut73dq 181 2 relationship relationship NN cord-329061-1xut73dq 181 3 to to IN cord-329061-1xut73dq 181 4 other other JJ cord-329061-1xut73dq 181 5 viruses virus NNS cord-329061-1xut73dq 181 6 . . . cord-329061-1xut73dq 182 1 Results result NNS cord-329061-1xut73dq 182 2 of of IN cord-329061-1xut73dq 182 3 these these DT cord-329061-1xut73dq 182 4 experiments experiment NNS cord-329061-1xut73dq 182 5 are be VBP cord-329061-1xut73dq 182 6 summarized summarize VBN cord-329061-1xut73dq 182 7 as as IN cord-329061-1xut73dq 182 8 follows follow VBZ cord-329061-1xut73dq 182 9 . . . cord-329061-1xut73dq 183 1 ( ( -LRB- cord-329061-1xut73dq 183 2 1 1 LS cord-329061-1xut73dq 183 3 ) ) -RRB- cord-329061-1xut73dq 183 4 Serum serum NN cord-329061-1xut73dq 183 5 - - HYPH cord-329061-1xut73dq 183 6 neutralization neutralization NN cord-329061-1xut73dq 183 7 tests test NNS cord-329061-1xut73dq 183 8 were be VBD cord-329061-1xut73dq 183 9 conducted conduct VBN cord-329061-1xut73dq 183 10 with with IN cord-329061-1xut73dq 183 11 strain strain NN cord-329061-1xut73dq 183 12 681 681 CD cord-329061-1xut73dq 183 13 SDA SDA NNP cord-329061-1xut73dq 183 14 virus virus NN cord-329061-1xut73dq 183 15 with with IN cord-329061-1xut73dq 183 16 immune immune JJ cord-329061-1xut73dq 183 17 sera sera NNP cord-329061-1xut73dq 183 18 to to IN cord-329061-1xut73dq 183 19 * * NFP cord-329061-1xut73dq 184 1 Names name NNS cord-329061-1xut73dq 184 2 of of IN cord-329061-1xut73dq 184 3 the the DT cord-329061-1xut73dq 184 4 individual individual JJ cord-329061-1xut73dq 184 5 arboviruses arbovirus NNS cord-329061-1xut73dq 184 6 will will MD cord-329061-1xut73dq 184 7 be be VB cord-329061-1xut73dq 184 8 furnished furnish VBN cord-329061-1xut73dq 184 9 upon upon IN cord-329061-1xut73dq 184 10 request request NN cord-329061-1xut73dq 184 11 . . . cord-329061-1xut73dq 185 1 Theiler Theiler NNP cord-329061-1xut73dq 185 2 encephalomyelitis encephalomyelitis NN cord-329061-1xut73dq 185 3 virus virus NN cord-329061-1xut73dq 185 4 ( ( -LRB- cord-329061-1xut73dq 185 5 strain strain NN cord-329061-1xut73dq 185 6 GD GD NNP cord-329061-1xut73dq 185 7 VII VII NNP cord-329061-1xut73dq 185 8 ) ) -RRB- cord-329061-1xut73dq 185 9 , , , cord-329061-1xut73dq 185 10 Sendai Sendai NNP cord-329061-1xut73dq 185 11 , , , cord-329061-1xut73dq 185 12 MVM MVM NNP cord-329061-1xut73dq 185 13 , , , cord-329061-1xut73dq 185 14 and and CC cord-329061-1xut73dq 185 15 mouse mouse NN cord-329061-1xut73dq 185 16 adenovirus adenovirus NN cord-329061-1xut73dq 185 17 were be VBD cord-329061-1xut73dq 185 18 negative negative JJ cord-329061-1xut73dq 185 19 . . . cord-329061-1xut73dq 186 1 Immune Immune NNP cord-329061-1xut73dq 186 2 sera sera NNP cord-329061-1xut73dq 186 3 to to IN cord-329061-1xut73dq 186 4 Toolan Toolan NNP cord-329061-1xut73dq 186 5 H H NNP cord-329061-1xut73dq 186 6 - - HYPH cord-329061-1xut73dq 186 7 l l NN cord-329061-1xut73dq 186 8 virus virus NN cord-329061-1xut73dq 186 9 , , , cord-329061-1xut73dq 186 10 Kilham Kilham NNP cord-329061-1xut73dq 186 11 rat rat NN cord-329061-1xut73dq 186 12 virus virus NN cord-329061-1xut73dq 186 13 , , , cord-329061-1xut73dq 186 14 and and CC cord-329061-1xut73dq 186 15 simian simian JJ cord-329061-1xut73dq 186 16 myxovirus myxovirus NNP cord-329061-1xut73dq 186 17 SV SV NNP cord-329061-1xut73dq 186 18 5 5 CD cord-329061-1xut73dq 186 19 ( ( -LRB- cord-329061-1xut73dq 186 20 at at IN cord-329061-1xut73dq 186 21 dilutions dilution NNS cord-329061-1xut73dq 186 22 of of IN cord-329061-1xut73dq 186 23 1 1 CD cord-329061-1xut73dq 186 24 : : SYM cord-329061-1xut73dq 186 25 5 5 LS cord-329061-1xut73dq 186 26 ) ) -RRB- cord-329061-1xut73dq 186 27 did do VBD cord-329061-1xut73dq 186 28 not not RB cord-329061-1xut73dq 186 29 neutralize neutralize VB cord-329061-1xut73dq 186 30 SDA SDA NNP cord-329061-1xut73dq 186 31 virus virus NN cord-329061-1xut73dq 186 32 . . . cord-329061-1xut73dq 187 1 ( ( -LRB- cord-329061-1xut73dq 187 2 2 2 LS cord-329061-1xut73dq 187 3 ) ) -RRB- cord-329061-1xut73dq 187 4 Sera Sera NNP cord-329061-1xut73dq 187 5 of of IN cord-329061-1xut73dq 187 6 mice mouse NNS cord-329061-1xut73dq 187 7 immune immune JJ cord-329061-1xut73dq 187 8 to to TO cord-329061-1xut73dq 187 9 strain strain VB cord-329061-1xut73dq 187 10 681 681 CD cord-329061-1xut73dq 187 11 were be VBD cord-329061-1xut73dq 187 12 tested test VBN cord-329061-1xut73dq 187 13 by by IN cord-329061-1xut73dq 187 14 CF CF NNP cord-329061-1xut73dq 187 15 at at IN cord-329061-1xut73dq 187 16 dilutions dilution NNS cord-329061-1xut73dq 187 17 of of IN cord-329061-1xut73dq 187 18 1 1 CD cord-329061-1xut73dq 187 19 : : SYM cord-329061-1xut73dq 187 20 4 4 CD cord-329061-1xut73dq 187 21 , , , cord-329061-1xut73dq 187 22 1 1 CD cord-329061-1xut73dq 187 23 : : SYM cord-329061-1xut73dq 187 24 8 8 CD cord-329061-1xut73dq 187 25 , , , cord-329061-1xut73dq 187 26 and and CC cord-329061-1xut73dq 187 27 1 1 CD cord-329061-1xut73dq 187 28 : : SYM cord-329061-1xut73dq 187 29 16 16 CD cord-329061-1xut73dq 187 30 for for IN cord-329061-1xut73dq 187 31 reactivity reactivity NN cord-329061-1xut73dq 187 32 to to IN cord-329061-1xut73dq 187 33 118 118 CD cord-329061-1xut73dq 187 34 viral viral JJ cord-329061-1xut73dq 187 35 antigens antigen NNS cord-329061-1xut73dq 187 36 . . . cord-329061-1xut73dq 188 1 These these DT cord-329061-1xut73dq 188 2 agents agent NNS cord-329061-1xut73dq 188 3 are be VBP cord-329061-1xut73dq 188 4 listed list VBN cord-329061-1xut73dq 188 5 in in IN cord-329061-1xut73dq 188 6 table table NN cord-329061-1xut73dq 188 7 3 3 CD cord-329061-1xut73dq 188 8 . . . cord-329061-1xut73dq 189 1 There there EX cord-329061-1xut73dq 189 2 was be VBD cord-329061-1xut73dq 189 3 no no DT cord-329061-1xut73dq 189 4 reaction reaction NN cord-329061-1xut73dq 189 5 , , , cord-329061-1xut73dq 189 6 indicating indicate VBG cord-329061-1xut73dq 189 7 absence absence NN cord-329061-1xut73dq 189 8 of of IN cord-329061-1xut73dq 189 9 antigenic antigenic JJ cord-329061-1xut73dq 189 10 relationship relationship NN cord-329061-1xut73dq 189 11 between between IN cord-329061-1xut73dq 189 12 our -PRON- PRP$ cord-329061-1xut73dq 189 13 agent agent NN cord-329061-1xut73dq 189 14 and and CC cord-329061-1xut73dq 189 15 these these DT cord-329061-1xut73dq 189 16 118 118 CD cord-329061-1xut73dq 189 17 agents agent NNS cord-329061-1xut73dq 189 18 under under IN cord-329061-1xut73dq 189 19 the the DT cord-329061-1xut73dq 189 20 conditions condition NNS cord-329061-1xut73dq 189 21 of of IN cord-329061-1xut73dq 189 22 the the DT cord-329061-1xut73dq 189 23 CF cf NN cord-329061-1xut73dq 189 24 test test NN cord-329061-1xut73dq 189 25 . . . cord-329061-1xut73dq 190 1 ( ( -LRB- cord-329061-1xut73dq 190 2 3 3 LS cord-329061-1xut73dq 190 3 ) ) -RRB- cord-329061-1xut73dq 191 1 A a DT cord-329061-1xut73dq 191 2 checkerboard checkerboard JJ cord-329061-1xut73dq 191 3 cross cross NN cord-329061-1xut73dq 191 4 - - JJ cord-329061-1xut73dq 191 5 CF cf NN cord-329061-1xut73dq 191 6 test test NN cord-329061-1xut73dq 191 7 was be VBD cord-329061-1xut73dq 191 8 performed perform VBN cord-329061-1xut73dq 191 9 , , , cord-329061-1xut73dq 191 10 using use VBG cord-329061-1xut73dq 191 11 two two CD cord-329061-1xut73dq 191 12 different different JJ cord-329061-1xut73dq 191 13 sera sera NNP cord-329061-1xut73dq 191 14 from from IN cord-329061-1xut73dq 191 15 mice mouse NNS cord-329061-1xut73dq 191 16 immune immune JJ cord-329061-1xut73dq 191 17 to to IN cord-329061-1xut73dq 191 18 SDA SDA NNP cord-329061-1xut73dq 191 19 agent agent NN cord-329061-1xut73dq 191 20 and and CC cord-329061-1xut73dq 191 21 commercial commercial JJ cord-329061-1xut73dq 191 22 MHV MHV NNP cord-329061-1xut73dq 191 23 antigen antigen NN cord-329061-1xut73dq 191 24 and and CC cord-329061-1xut73dq 191 25 corresponding corresponding JJ cord-329061-1xut73dq 191 26 immune immune JJ cord-329061-1xut73dq 191 27 serum serum NN cord-329061-1xut73dq 191 28 . . . cord-329061-1xut73dq 192 1 On on IN cord-329061-1xut73dq 192 2 the the DT cord-329061-1xut73dq 192 3 Table Table NNP cord-329061-1xut73dq 192 4 5 5 CD cord-329061-1xut73dq 192 5 . . . cord-329061-1xut73dq 193 1 Results result NNS cord-329061-1xut73dq 193 2 of of IN cord-329061-1xut73dq 193 3 cross cross NN cord-329061-1xut73dq 193 4 - - JJ cord-329061-1xut73dq 193 5 neutralization neutralization JJ cord-329061-1xut73dq 193 6 tests test NNS cord-329061-1xut73dq 193 7 with with IN cord-329061-1xut73dq 193 8 sialodacryoadenitis sialodacryoadenitis NNP cord-329061-1xut73dq 193 9 virus virus NN cord-329061-1xut73dq 193 10 ( ( -LRB- cord-329061-1xut73dq 193 11 SDA SDA NNP cord-329061-1xut73dq 193 12 ) ) -RRB- cord-329061-1xut73dq 193 13 and and CC cord-329061-1xut73dq 193 14 rat rat NN cord-329061-1xut73dq 193 15 coronavirus coronavirus NN cord-329061-1xut73dq 193 16 ( ( -LRB- cord-329061-1xut73dq 193 17 ReV ReV NNP cord-329061-1xut73dq 193 18 ) ) -RRB- cord-329061-1xut73dq 193 19 and and CC cord-329061-1xut73dq 193 20 respective respective JJ cord-329061-1xut73dq 193 21 immune immune JJ cord-329061-1xut73dq 193 22 sera sera NNP cord-329061-1xut73dq 193 23 . . . cord-329061-1xut73dq 194 1 ( ( -LRB- cord-329061-1xut73dq 194 2 4 4 LS cord-329061-1xut73dq 194 3 ) ) -RRB- cord-329061-1xut73dq 194 4 Cross cross NN cord-329061-1xut73dq 194 5 - - NN cord-329061-1xut73dq 194 6 neutralization neutralization NN cord-329061-1xut73dq 194 7 tests test NNS cord-329061-1xut73dq 194 8 were be VBD cord-329061-1xut73dq 194 9 performed perform VBN cord-329061-1xut73dq 194 10 with with IN cord-329061-1xut73dq 194 11 Parker Parker NNP cord-329061-1xut73dq 194 12 's 's POS cord-329061-1xut73dq 194 13 rat rat NN cord-329061-1xut73dq 194 14 coronavirus coronavirus NN cord-329061-1xut73dq 194 15 and and CC cord-329061-1xut73dq 194 16 strain strain VB cord-329061-1xut73dq 194 17 681 681 CD cord-329061-1xut73dq 194 18 , , , cord-329061-1xut73dq 194 19 using use VBG cord-329061-1xut73dq 194 20 both both CC cord-329061-1xut73dq 194 21 homologous homologous JJ cord-329061-1xut73dq 194 22 and and CC cord-329061-1xut73dq 194 23 heterologous heterologous JJ cord-329061-1xut73dq 194 24 immune immune NNP cord-329061-1xut73dq 194 25 sera sera NNP cord-329061-1xut73dq 194 26 . . . cord-329061-1xut73dq 195 1 As as IN cord-329061-1xut73dq 195 2 shown show VBN cord-329061-1xut73dq 195 3 in in IN cord-329061-1xut73dq 195 4 table table NN cord-329061-1xut73dq 195 5 5 5 CD cord-329061-1xut73dq 195 6 , , , cord-329061-1xut73dq 195 7 there there EX cord-329061-1xut73dq 195 8 is be VBZ cord-329061-1xut73dq 195 9 cross cross NN cord-329061-1xut73dq 195 10 - - NN cord-329061-1xut73dq 195 11 neutralization neutralization NN cord-329061-1xut73dq 195 12 , , , cord-329061-1xut73dq 195 13 but but CC cord-329061-1xut73dq 195 14 there there EX cord-329061-1xut73dq 195 15 are be VBP cord-329061-1xut73dq 195 16 also also RB cord-329061-1xut73dq 195 17 antigenic antigenic JJ cord-329061-1xut73dq 195 18 differences difference NNS cord-329061-1xut73dq 195 19 between between IN cord-329061-1xut73dq 195 20 these these DT cord-329061-1xut73dq 195 21 viruses virus NNS cord-329061-1xut73dq 195 22 . . . cord-329061-1xut73dq 196 1 Induction induction NN cord-329061-1xut73dq 196 2 of of IN cord-329061-1xut73dq 196 3 disease disease NN cord-329061-1xut73dq 196 4 in in IN cord-329061-1xut73dq 196 5 weanling weanle VBG cord-329061-1xut73dq 196 6 mice mouse NNS cord-329061-1xut73dq 196 7 . . . cord-329061-1xut73dq 197 1 Neither neither DT cord-329061-1xut73dq 197 2 overt overt JJ cord-329061-1xut73dq 197 3 illness illness NN cord-329061-1xut73dq 197 4 nor nor CC cord-329061-1xut73dq 197 5 any any DT cord-329061-1xut73dq 197 6 gross gross JJ cord-329061-1xut73dq 197 7 or or CC cord-329061-1xut73dq 197 8 histologic histologic JJ cord-329061-1xut73dq 197 9 evidence evidence NN cord-329061-1xut73dq 197 10 of of IN cord-329061-1xut73dq 197 11 lesions lesion NNS cord-329061-1xut73dq 197 12 was be VBD cord-329061-1xut73dq 197 13 associated associate VBN cord-329061-1xut73dq 197 14 with with IN cord-329061-1xut73dq 197 15 this this DT cord-329061-1xut73dq 197 16 agent agent NN cord-329061-1xut73dq 197 17 . . . cord-329061-1xut73dq 198 1 More more RBR cord-329061-1xut73dq 198 2 specifically specifically RB cord-329061-1xut73dq 198 3 , , , cord-329061-1xut73dq 198 4 there there EX cord-329061-1xut73dq 198 5 were be VBD cord-329061-1xut73dq 198 6 no no DT cord-329061-1xut73dq 198 7 lesions lesion NNS cord-329061-1xut73dq 198 8 compatible compatible JJ cord-329061-1xut73dq 198 9 with with IN cord-329061-1xut73dq 198 10 infection infection NN cord-329061-1xut73dq 198 11 by by IN cord-329061-1xut73dq 198 12 MHV MHV NNP cord-329061-1xut73dq 198 13 either either CC cord-329061-1xut73dq 198 14 in in IN cord-329061-1xut73dq 198 15 the the DT cord-329061-1xut73dq 198 16 group group NN cord-329061-1xut73dq 198 17 treated treat VBN cord-329061-1xut73dq 198 18 with with IN cord-329061-1xut73dq 198 19 cortisone cortisone NN cord-329061-1xut73dq 198 20 or or CC cord-329061-1xut73dq 198 21 in in IN cord-329061-1xut73dq 198 22 the the DT cord-329061-1xut73dq 198 23 untreated untreated JJ cord-329061-1xut73dq 198 24 group group NN cord-329061-1xut73dq 198 25 . . . cord-329061-1xut73dq 199 1 Production production NN cord-329061-1xut73dq 199 2 of of IN cord-329061-1xut73dq 199 3 SDA SDA NNP cord-329061-1xut73dq 199 4 in in IN cord-329061-1xut73dq 199 5 susceptible susceptible JJ cord-329061-1xut73dq 199 6 rats rat NNS cord-329061-1xut73dq 199 7 by by IN cord-329061-1xut73dq 199 8 mousebrain mousebrain NN cord-329061-1xut73dq 199 9 - - HYPH cord-329061-1xut73dq 199 10 adapted adapt VBN cord-329061-1xut73dq 199 11 strain strain NN cord-329061-1xut73dq 199 12 681 681 CD cord-329061-1xut73dq 199 13 . . . cord-329061-1xut73dq 200 1 There there EX cord-329061-1xut73dq 200 2 was be VBD cord-329061-1xut73dq 200 3 no no DT cord-329061-1xut73dq 200 4 overt overt JJ cord-329061-1xut73dq 200 5 illness illness NN cord-329061-1xut73dq 200 6 in in IN cord-329061-1xut73dq 200 7 any any DT cord-329061-1xut73dq 200 8 rat rat NN cord-329061-1xut73dq 200 9 , , , cord-329061-1xut73dq 200 10 and and CC cord-329061-1xut73dq 200 11 at at IN cord-329061-1xut73dq 200 12 necropsy necropsy JJ cord-329061-1xut73dq 200 13 all all DT cord-329061-1xut73dq 200 14 organs organ NNS cord-329061-1xut73dq 200 15 , , , cord-329061-1xut73dq 200 16 including include VBG cord-329061-1xut73dq 200 17 the the DT cord-329061-1xut73dq 200 18 lacrimal lacrimal NN cord-329061-1xut73dq 200 19 and and CC cord-329061-1xut73dq 200 20 salivary salivary NN cord-329061-1xut73dq 200 21 glands gland NNS cord-329061-1xut73dq 200 22 , , , cord-329061-1xut73dq 200 23 were be VBD cord-329061-1xut73dq 200 24 grossly grossly RB cord-329061-1xut73dq 200 25 normal normal JJ cord-329061-1xut73dq 200 26 . . . cord-329061-1xut73dq 201 1 Histopathologic Histopathologic NNP cord-329061-1xut73dq 201 2 examination examination NN cord-329061-1xut73dq 201 3 of of IN cord-329061-1xut73dq 201 4 the the DT cord-329061-1xut73dq 201 5 Harderian Harderian NNP cord-329061-1xut73dq 201 6 , , , cord-329061-1xut73dq 201 7 exorbital exorbital JJ cord-329061-1xut73dq 201 8 , , , cord-329061-1xut73dq 201 9 parotid parotid NN cord-329061-1xut73dq 201 10 , , , cord-329061-1xut73dq 201 11 and and CC cord-329061-1xut73dq 201 12 submaxillary submaxillary JJ cord-329061-1xut73dq 201 13 salivary salivary JJ cord-329061-1xut73dq 201 14 glands gland NNS cord-329061-1xut73dq 201 15 showed show VBD cord-329061-1xut73dq 201 16 evidence evidence NN cord-329061-1xut73dq 201 17 of of IN cord-329061-1xut73dq 201 18 SDA SDA NNP cord-329061-1xut73dq 201 19 . . . cord-329061-1xut73dq 202 1 There there EX cord-329061-1xut73dq 202 2 was be VBD cord-329061-1xut73dq 202 3 considerable considerable JJ cord-329061-1xut73dq 202 4 variation variation NN cord-329061-1xut73dq 202 5 in in IN cord-329061-1xut73dq 202 6 the the DT cord-329061-1xut73dq 202 7 severity severity NN cord-329061-1xut73dq 202 8 of of IN cord-329061-1xut73dq 202 9 the the DT cord-329061-1xut73dq 202 10 reaction reaction NN cord-329061-1xut73dq 202 11 of of IN cord-329061-1xut73dq 202 12 affected affected JJ cord-329061-1xut73dq 202 13 glands gland NNS cord-329061-1xut73dq 202 14 . . . cord-329061-1xut73dq 203 1 In in IN cord-329061-1xut73dq 203 2 general general JJ cord-329061-1xut73dq 203 3 , , , cord-329061-1xut73dq 203 4 lesions lesion NNS cord-329061-1xut73dq 203 5 were be VBD cord-329061-1xut73dq 203 6 most most RBS cord-329061-1xut73dq 203 7 numerous numerous JJ cord-329061-1xut73dq 203 8 and and CC cord-329061-1xut73dq 203 9 most most RBS cord-329061-1xut73dq 203 10 severe severe JJ cord-329061-1xut73dq 203 11 in in IN cord-329061-1xut73dq 203 12 the the DT cord-329061-1xut73dq 203 13 parotid parotid NN cord-329061-1xut73dq 203 14 and and CC cord-329061-1xut73dq 203 15 exorbital exorbital JJ cord-329061-1xut73dq 203 16 glands gland NNS cord-329061-1xut73dq 203 17 . . . cord-329061-1xut73dq 204 1 Two two CD cord-329061-1xut73dq 204 2 important important JJ cord-329061-1xut73dq 204 3 features feature NNS cord-329061-1xut73dq 204 4 of of IN cord-329061-1xut73dq 204 5 this this DT cord-329061-1xut73dq 204 6 study study NN cord-329061-1xut73dq 204 7 were be VBD cord-329061-1xut73dq 204 8 the the DT cord-329061-1xut73dq 204 9 relative relative JJ cord-329061-1xut73dq 204 10 severity severity NN cord-329061-1xut73dq 204 11 of of IN cord-329061-1xut73dq 204 12 lesions lesion NNS cord-329061-1xut73dq 204 13 in in IN cord-329061-1xut73dq 204 14 the the DT cord-329061-1xut73dq 204 15 parotid parotid JJ cord-329061-1xut73dq 204 16 salivary salivary NN cord-329061-1xut73dq 204 17 glands gland NNS cord-329061-1xut73dq 204 18 and and CC cord-329061-1xut73dq 204 19 the the DT cord-329061-1xut73dq 204 20 failure failure NN cord-329061-1xut73dq 204 21 to to TO cord-329061-1xut73dq 204 22 isolate isolate VB cord-329061-1xut73dq 204 23 an an DT cord-329061-1xut73dq 204 24 agent agent NN cord-329061-1xut73dq 204 25 pathogenic pathogenic JJ cord-329061-1xut73dq 204 26 for for IN cord-329061-1xut73dq 204 27 mice mouse NNS cord-329061-1xut73dq 204 28 from from IN cord-329061-1xut73dq 204 29 these these DT cord-329061-1xut73dq 204 30 animals animal NNS cord-329061-1xut73dq 204 31 . . . cord-329061-1xut73dq 205 1 These these DT cord-329061-1xut73dq 205 2 findings finding NNS cord-329061-1xut73dq 205 3 suggest suggest VBP cord-329061-1xut73dq 205 4 that that IN cord-329061-1xut73dq 205 5 the the DT cord-329061-1xut73dq 205 6 mouse mouse NN cord-329061-1xut73dq 205 7 - - HYPH cord-329061-1xut73dq 205 8 brainadapted brainadapte VBN cord-329061-1xut73dq 205 9 virus virus NN cord-329061-1xut73dq 205 10 caused cause VBD cord-329061-1xut73dq 205 11 mild mild JJ cord-329061-1xut73dq 205 12 but but CC cord-329061-1xut73dq 205 13 definite definite JJ cord-329061-1xut73dq 205 14 lesions lesion NNS cord-329061-1xut73dq 205 15 compatible compatible JJ cord-329061-1xut73dq 205 16 with with IN cord-329061-1xut73dq 205 17 SDA SDA NNP cord-329061-1xut73dq 205 18 . . . cord-329061-1xut73dq 206 1 It -PRON- PRP cord-329061-1xut73dq 206 2 has have VBZ cord-329061-1xut73dq 206 3 been be VBN cord-329061-1xut73dq 206 4 approximately approximately RB cord-329061-1xut73dq 206 5 10 10 CD cord-329061-1xut73dq 206 6 years year NNS cord-329061-1xut73dq 206 7 since since IN cord-329061-1xut73dq 206 8 the the DT cord-329061-1xut73dq 206 9 first first JJ cord-329061-1xut73dq 206 10 recognized recognize VBN cord-329061-1xut73dq 206 11 outbreak outbreak NN cord-329061-1xut73dq 206 12 of of IN cord-329061-1xut73dq 206 13 SDA SDA NNP cord-329061-1xut73dq 206 14 in in IN cord-329061-1xut73dq 206 15 rats rat NNS cord-329061-1xut73dq 206 16 . . . cord-329061-1xut73dq 207 1 On on IN cord-329061-1xut73dq 207 2 the the DT cord-329061-1xut73dq 207 3 basis basis NN cord-329061-1xut73dq 207 4 of of IN cord-329061-1xut73dq 207 5 the the DT cord-329061-1xut73dq 207 6 information information NN cord-329061-1xut73dq 207 7 presented present VBN cord-329061-1xut73dq 207 8 in in IN cord-329061-1xut73dq 207 9 this this DT cord-329061-1xut73dq 207 10 report report NN cord-329061-1xut73dq 207 11 , , , cord-329061-1xut73dq 207 12 it -PRON- PRP cord-329061-1xut73dq 207 13 is be VBZ cord-329061-1xut73dq 207 14 concluded conclude VBN cord-329061-1xut73dq 207 15 that that IN cord-329061-1xut73dq 207 16 a a DT cord-329061-1xut73dq 207 17 viral viral JJ cord-329061-1xut73dq 207 18 agent agent NN cord-329061-1xut73dq 207 19 causing cause VBG cord-329061-1xut73dq 207 20 this this DT cord-329061-1xut73dq 207 21 disease disease NN cord-329061-1xut73dq 207 22 in in IN cord-329061-1xut73dq 207 23 rats rat NNS cord-329061-1xut73dq 207 24 has have VBZ cord-329061-1xut73dq 207 25 been be VBN cord-329061-1xut73dq 207 26 isolated isolate VBN cord-329061-1xut73dq 207 27 and and CC cord-329061-1xut73dq 207 28 adapted adapt VBN cord-329061-1xut73dq 207 29 to to TO cord-329061-1xut73dq 207 30 grow grow VB cord-329061-1xut73dq 207 31 in in IN cord-329061-1xut73dq 207 32 brains brain NNS cord-329061-1xut73dq 207 33 of of IN cord-329061-1xut73dq 207 34 infant infant NN cord-329061-1xut73dq 207 35 mice mouse NNS cord-329061-1xut73dq 207 36 and and CC cord-329061-1xut73dq 207 37 in in IN cord-329061-1xut73dq 207 38 primary primary JJ cord-329061-1xut73dq 207 39 rat rat NN cord-329061-1xut73dq 207 40 - - HYPH cord-329061-1xut73dq 207 41 kidney kidney NN cord-329061-1xut73dq 207 42 cultures culture NNS cord-329061-1xut73dq 207 43 . . . cord-329061-1xut73dq 208 1 The the DT cord-329061-1xut73dq 208 2 agent agent NN cord-329061-1xut73dq 208 3 probably probably RB cord-329061-1xut73dq 208 4 has have VBZ cord-329061-1xut73dq 208 5 RNA RNA NNP cord-329061-1xut73dq 208 6 as as IN cord-329061-1xut73dq 208 7 its -PRON- PRP$ cord-329061-1xut73dq 208 8 nucleic nucleic JJ cord-329061-1xut73dq 208 9 acid acid NN cord-329061-1xut73dq 208 10 and and CC cord-329061-1xut73dq 208 11 is be VBZ cord-329061-1xut73dq 208 12 sensitive sensitive JJ cord-329061-1xut73dq 208 13 to to IN cord-329061-1xut73dq 208 14 lipid lipid NN cord-329061-1xut73dq 208 15 solvents solvent NNS cord-329061-1xut73dq 208 16 . . . cord-329061-1xut73dq 209 1 It -PRON- PRP cord-329061-1xut73dq 209 2 is be VBZ cord-329061-1xut73dq 209 3 antigenically antigenically RB cord-329061-1xut73dq 209 4 related relate VBN cord-329061-1xut73dq 209 5 to to IN cord-329061-1xut73dq 209 6 the the DT cord-329061-1xut73dq 209 7 rat rat NN cord-329061-1xut73dq 209 8 coronavirus coronavirus NN cord-329061-1xut73dq 209 9 of of IN cord-329061-1xut73dq 209 10 Parker Parker NNP cord-329061-1xut73dq 209 11 and and CC cord-329061-1xut73dq 209 12 to to IN cord-329061-1xut73dq 209 13 mouse mouse NN cord-329061-1xut73dq 209 14 - - HYPH cord-329061-1xut73dq 209 15 hepatitis hepatitis NN cord-329061-1xut73dq 209 16 virus virus NN cord-329061-1xut73dq 209 17 . . . cord-329061-1xut73dq 210 1 It -PRON- PRP cord-329061-1xut73dq 210 2 apparently apparently RB cord-329061-1xut73dq 210 3 multiplies multiply VBZ cord-329061-1xut73dq 210 4 in in IN cord-329061-1xut73dq 210 5 the the DT cord-329061-1xut73dq 210 6 cytoplasm cytoplasm NN cord-329061-1xut73dq 210 7 and and CC cord-329061-1xut73dq 210 8 forms form NNS cord-329061-1xut73dq 210 9 multinucleated multinucleate VBN cord-329061-1xut73dq 210 10 giant giant JJ cord-329061-1xut73dq 210 11 cells cell NNS cord-329061-1xut73dq 210 12 in in IN cord-329061-1xut73dq 210 13 tissue tissue NN cord-329061-1xut73dq 210 14 culture culture NN cord-329061-1xut73dq 210 15 . . . cord-329061-1xut73dq 211 1 Some some DT cord-329061-1xut73dq 211 2 coronaviruses coronaviruse NNS cord-329061-1xut73dq 211 3 are be VBP cord-329061-1xut73dq 211 4 acid acid JJ cord-329061-1xut73dq 211 5 labile labile JJ cord-329061-1xut73dq 211 6 , , , cord-329061-1xut73dq 211 7 but but CC cord-329061-1xut73dq 211 8 the the DT cord-329061-1xut73dq 211 9 SDA SDA NNP cord-329061-1xut73dq 211 10 agent agent NN cord-329061-1xut73dq 211 11 is be VBZ cord-329061-1xut73dq 211 12 relatively relatively RB cord-329061-1xut73dq 211 13 stable stable JJ cord-329061-1xut73dq 211 14 at at IN cord-329061-1xut73dq 211 15 pH pH NNP cord-329061-1xut73dq 211 16 3.0 3.0 CD cord-329061-1xut73dq 211 17 . . . cord-329061-1xut73dq 212 1 The the DT cord-329061-1xut73dq 212 2 virus virus NN cord-329061-1xut73dq 212 3 of of IN cord-329061-1xut73dq 212 4 transmissible transmissible JJ cord-329061-1xut73dq 212 5 gastroenteritis gastroenteritis NN cord-329061-1xut73dq 212 6 is be VBZ cord-329061-1xut73dq 212 7 also also RB cord-329061-1xut73dq 212 8 stable stable JJ cord-329061-1xut73dq 212 9 at at IN cord-329061-1xut73dq 212 10 pH pH NNP cord-329061-1xut73dq 212 11 3.0 3.0 CD cord-329061-1xut73dq 212 12 , , , cord-329061-1xut73dq 212 13 yet yet CC cord-329061-1xut73dq 212 14 is be VBZ cord-329061-1xut73dq 212 15 considered consider VBN cord-329061-1xut73dq 212 16 a a DT cord-329061-1xut73dq 212 17 coronavirus coronavirus NN cord-329061-1xut73dq 212 18 [ [ -LRB- cord-329061-1xut73dq 212 19 17 17 CD cord-329061-1xut73dq 212 20 ] ] -RRB- cord-329061-1xut73dq 212 21 . . . cord-329061-1xut73dq 213 1 Thus thus RB cord-329061-1xut73dq 213 2 pH pH NNP cord-329061-1xut73dq 213 3 stability stability NN cord-329061-1xut73dq 213 4 may may MD cord-329061-1xut73dq 213 5 be be VB cord-329061-1xut73dq 213 6 a a DT cord-329061-1xut73dq 213 7 variable variable JJ cord-329061-1xut73dq 213 8 feature feature NN cord-329061-1xut73dq 213 9 of of IN cord-329061-1xut73dq 213 10 this this DT cord-329061-1xut73dq 213 11 group group NN cord-329061-1xut73dq 213 12 . . . cord-329061-1xut73dq 214 1 On on IN cord-329061-1xut73dq 214 2 the the DT cord-329061-1xut73dq 214 3 basis basis NN cord-329061-1xut73dq 214 4 of of IN cord-329061-1xut73dq 214 5 this this DT cord-329061-1xut73dq 214 6 information information NN cord-329061-1xut73dq 214 7 , , , cord-329061-1xut73dq 214 8 we -PRON- PRP cord-329061-1xut73dq 214 9 conclude conclude VBP cord-329061-1xut73dq 214 10 that that IN cord-329061-1xut73dq 214 11 this this DT cord-329061-1xut73dq 214 12 virus virus NN cord-329061-1xut73dq 214 13 belongs belong VBZ cord-329061-1xut73dq 214 14 to to IN cord-329061-1xut73dq 214 15 the the DT cord-329061-1xut73dq 214 16 corona corona NN cord-329061-1xut73dq 214 17 group group NN cord-329061-1xut73dq 214 18 , , , cord-329061-1xut73dq 214 19 even even RB cord-329061-1xut73dq 214 20 though though IN cord-329061-1xut73dq 214 21 information information NN cord-329061-1xut73dq 214 22 on on IN cord-329061-1xut73dq 214 23 the the DT cord-329061-1xut73dq 214 24 morphology morphology NN cord-329061-1xut73dq 214 25 of of IN cord-329061-1xut73dq 214 26 the the DT cord-329061-1xut73dq 214 27 negatively negatively RB cord-329061-1xut73dq 214 28 stained stain VBN cord-329061-1xut73dq 214 29 viral viral JJ cord-329061-1xut73dq 214 30 particle particle NN cord-329061-1xut73dq 214 31 and and CC cord-329061-1xut73dq 214 32 its -PRON- PRP$ cord-329061-1xut73dq 214 33 mode mode NN cord-329061-1xut73dq 214 34 of of IN cord-329061-1xut73dq 214 35 replication replication NN cord-329061-1xut73dq 214 36 in in IN cord-329061-1xut73dq 214 37 cells cell NNS cord-329061-1xut73dq 214 38 ( ( -LRB- cord-329061-1xut73dq 214 39 as as IN cord-329061-1xut73dq 214 40 determined determine VBN cord-329061-1xut73dq 214 41 by by IN cord-329061-1xut73dq 214 42 electron electron NN cord-329061-1xut73dq 214 43 microscopy microscopy NNP cord-329061-1xut73dq 214 44 ) ) -RRB- cord-329061-1xut73dq 214 45 has have VBZ cord-329061-1xut73dq 214 46 yet yet RB cord-329061-1xut73dq 214 47 to to TO cord-329061-1xut73dq 214 48 be be VB cord-329061-1xut73dq 214 49 obtained obtain VBN cord-329061-1xut73dq 214 50 . . . cord-329061-1xut73dq 215 1 In in IN cord-329061-1xut73dq 215 2 the the DT cord-329061-1xut73dq 215 3 affected affected JJ cord-329061-1xut73dq 215 4 tissues tissue NNS cord-329061-1xut73dq 215 5 , , , cord-329061-1xut73dq 215 6 the the DT cord-329061-1xut73dq 215 7 particles particle NNS cord-329061-1xut73dq 215 8 are be VBP cord-329061-1xut73dq 215 9 frequently frequently RB cord-329061-1xut73dq 215 10 found find VBN cord-329061-1xut73dq 215 11 in in IN cord-329061-1xut73dq 215 12 cytoplasmic cytoplasmic JJ cord-329061-1xut73dq 215 13 vesicles vesicle NNS cord-329061-1xut73dq 215 14 that that WDT cord-329061-1xut73dq 215 15 eventually eventually RB cord-329061-1xut73dq 215 16 contain contain VBP cord-329061-1xut73dq 215 17 lysosomal lysosomal JJ cord-329061-1xut73dq 215 18 activity activity NN cord-329061-1xut73dq 215 19 ( ( -LRB- cord-329061-1xut73dq 215 20 A. A. NNP cord-329061-1xut73dq 215 21 M. M. NNP cord-329061-1xut73dq 215 22 Jonas Jonas NNP cord-329061-1xut73dq 215 23 , , , cord-329061-1xut73dq 215 24 unpublished unpublished JJ cord-329061-1xut73dq 215 25 observation observation NN cord-329061-1xut73dq 215 26 ) ) -RRB- cord-329061-1xut73dq 215 27 . . . cord-329061-1xut73dq 216 1 The the DT cord-329061-1xut73dq 216 2 titer titer NN cord-329061-1xut73dq 216 3 of of IN cord-329061-1xut73dq 216 4 infectious infectious JJ cord-329061-1xut73dq 216 5 virus virus NN cord-329061-1xut73dq 216 6 in in IN cord-329061-1xut73dq 216 7 mouse mouse NN cord-329061-1xut73dq 216 8 brain brain NN cord-329061-1xut73dq 216 9 was be VBD cord-329061-1xut73dq 216 10 not not RB cord-329061-1xut73dq 216 11 increased increase VBN cord-329061-1xut73dq 216 12 by by IN cord-329061-1xut73dq 216 13 serial serial JJ cord-329061-1xut73dq 216 14 passage passage NN cord-329061-1xut73dq 216 15 . . . cord-329061-1xut73dq 217 1 This this DT cord-329061-1xut73dq 217 2 may may MD cord-329061-1xut73dq 217 3 be be VB cord-329061-1xut73dq 217 4 a a DT cord-329061-1xut73dq 217 5 reflection reflection NN cord-329061-1xut73dq 217 6 of of IN cord-329061-1xut73dq 217 7 the the DT cord-329061-1xut73dq 217 8 facts fact NNS cord-329061-1xut73dq 217 9 that that WDT cord-329061-1xut73dq 217 10 only only RB cord-329061-1xut73dq 217 11 selected select VBN cord-329061-1xut73dq 217 12 cells cell NNS cord-329061-1xut73dq 217 13 are be VBP cord-329061-1xut73dq 217 14 involved involve VBN cord-329061-1xut73dq 217 15 in in IN cord-329061-1xut73dq 217 16 viral viral JJ cord-329061-1xut73dq 217 17 multiplication multiplication NN cord-329061-1xut73dq 217 18 and and CC cord-329061-1xut73dq 217 19 that that IN cord-329061-1xut73dq 217 20 there there EX cord-329061-1xut73dq 217 21 may may MD cord-329061-1xut73dq 217 22 be be VB cord-329061-1xut73dq 217 23 a a DT cord-329061-1xut73dq 217 24 low low JJ cord-329061-1xut73dq 217 25 yield yield NN cord-329061-1xut73dq 217 26 of of IN cord-329061-1xut73dq 217 27 infectious infectious JJ cord-329061-1xut73dq 217 28 virus virus NN cord-329061-1xut73dq 217 29 per per IN cord-329061-1xut73dq 217 30 infected infected JJ cord-329061-1xut73dq 217 31 cell cell NN cord-329061-1xut73dq 217 32 . . . cord-329061-1xut73dq 218 1 ( ( -LRB- cord-329061-1xut73dq 218 2 The the DT cord-329061-1xut73dq 218 3 former former JJ cord-329061-1xut73dq 218 4 supposition supposition NN cord-329061-1xut73dq 218 5 has have VBZ cord-329061-1xut73dq 218 6 been be VBN cord-329061-1xut73dq 218 7 confirmed confirm VBN cord-329061-1xut73dq 218 8 by by IN cord-329061-1xut73dq 218 9 detailed detailed JJ cord-329061-1xut73dq 218 10 histopathologic histopathologic JJ cord-329061-1xut73dq 218 11 study study NN cord-329061-1xut73dq 218 12 supplemented supplement VBN cord-329061-1xut73dq 218 13 by by IN cord-329061-1xut73dq 218 14 fluorescent fluorescent JJ cord-329061-1xut73dq 218 15 - - HYPH cord-329061-1xut73dq 218 16 antibody antibody NN cord-329061-1xut73dq 218 17 tracing tracing NN cord-329061-1xut73dq 218 18 of of IN cord-329061-1xut73dq 218 19 infected infected JJ cord-329061-1xut73dq 218 20 cells cell NNS cord-329061-1xut73dq 218 21 . . . cord-329061-1xut73dq 218 22 ) ) -RRB- cord-329061-1xut73dq 219 1 When when WRB cord-329061-1xut73dq 219 2 working work VBG cord-329061-1xut73dq 219 3 with with IN cord-329061-1xut73dq 219 4 mouse mouse NN cord-329061-1xut73dq 219 5 - - HYPH cord-329061-1xut73dq 219 6 adapted adapt VBN cord-329061-1xut73dq 219 7 viruses virus NNS cord-329061-1xut73dq 219 8 of of IN cord-329061-1xut73dq 219 9 rats rat NNS cord-329061-1xut73dq 219 10 , , , cord-329061-1xut73dq 219 11 it -PRON- PRP cord-329061-1xut73dq 219 12 is be VBZ cord-329061-1xut73dq 219 13 essential essential JJ cord-329061-1xut73dq 219 14 to to TO cord-329061-1xut73dq 219 15 prove prove VB cord-329061-1xut73dq 219 16 that that IN cord-329061-1xut73dq 219 17 one one NN cord-329061-1xut73dq 219 18 has have VBZ cord-329061-1xut73dq 219 19 not not RB cord-329061-1xut73dq 219 20 picked pick VBN cord-329061-1xut73dq 219 21 up up RP cord-329061-1xut73dq 219 22 agents agent NNS cord-329061-1xut73dq 219 23 from from IN cord-329061-1xut73dq 219 24 mice mouse NNS cord-329061-1xut73dq 219 25 . . . cord-329061-1xut73dq 220 1 Evidence evidence NN cord-329061-1xut73dq 220 2 against against IN cord-329061-1xut73dq 220 3 this this DT cord-329061-1xut73dq 220 4 possibility possibility NN cord-329061-1xut73dq 220 5 includes include VBZ cord-329061-1xut73dq 220 6 the the DT cord-329061-1xut73dq 220 7 following following NN cord-329061-1xut73dq 220 8 . . . cord-329061-1xut73dq 221 1 ( ( -LRB- cord-329061-1xut73dq 221 2 l l NN cord-329061-1xut73dq 221 3 ) ) -RRB- cord-329061-1xut73dq 221 4 Sickness sickness NN cord-329061-1xut73dq 221 5 in in IN cord-329061-1xut73dq 221 6 mice mice NN cord-329061-1xut73dq 221 7 is be VBZ cord-329061-1xut73dq 221 8 produced produce VBN cord-329061-1xut73dq 221 9 only only RB cord-329061-1xut73dq 221 10 when when WRB cord-329061-1xut73dq 221 11 the the DT cord-329061-1xut73dq 221 12 animals animal NNS cord-329061-1xut73dq 221 13 are be VBP cord-329061-1xut73dq 221 14 inoculated inoculate VBN cord-329061-1xut73dq 221 15 with with IN cord-329061-1xut73dq 221 16 a a DT cord-329061-1xut73dq 221 17 suspension suspension NN cord-329061-1xut73dq 221 18 of of IN cord-329061-1xut73dq 221 19 infected infected JJ cord-329061-1xut73dq 221 20 salivary salivary NN cord-329061-1xut73dq 221 21 gland gland NN cord-329061-1xut73dq 221 22 and and CC cord-329061-1xut73dq 221 23 not not RB cord-329061-1xut73dq 221 24 when when WRB cord-329061-1xut73dq 221 25 normal normal JJ cord-329061-1xut73dq 221 26 salivary salivary NN cord-329061-1xut73dq 221 27 gland gland NN cord-329061-1xut73dq 221 28 is be VBZ cord-329061-1xut73dq 221 29 used use VBN cord-329061-1xut73dq 221 30 . . . cord-329061-1xut73dq 222 1 ( ( -LRB- cord-329061-1xut73dq 222 2 2 2 LS cord-329061-1xut73dq 222 3 ) ) -RRB- cord-329061-1xut73dq 222 4 Agents agent NNS cord-329061-1xut73dq 222 5 pathogenic pathogenic JJ cord-329061-1xut73dq 222 6 for for IN cord-329061-1xut73dq 222 7 mice mouse NNS cord-329061-1xut73dq 222 8 were be VBD cord-329061-1xut73dq 222 9 not not RB cord-329061-1xut73dq 222 10 recovered recover VBN cord-329061-1xut73dq 222 11 when when WRB cord-329061-1xut73dq 222 12 brains brain NNS cord-329061-1xut73dq 222 13 from from IN cord-329061-1xut73dq 222 14 normal normal JJ cord-329061-1xut73dq 222 15 mice mouse NNS cord-329061-1xut73dq 222 16 were be VBD cord-329061-1xut73dq 222 17 passaged passage VBN cord-329061-1xut73dq 222 18 consecutively consecutively RB cord-329061-1xut73dq 222 19 several several JJ cord-329061-1xut73dq 222 20 times time NNS cord-329061-1xut73dq 222 21 by by IN cord-329061-1xut73dq 222 22 the the DT cord-329061-1xut73dq 222 23 intracerebral intracerebral NNP cord-329061-1xut73dq 222 24 129 129 CD cord-329061-1xut73dq 222 25 route route NN cord-329061-1xut73dq 222 26 in in IN cord-329061-1xut73dq 222 27 mice mouse NNS cord-329061-1xut73dq 222 28 . . . cord-329061-1xut73dq 223 1 ( ( -LRB- cord-329061-1xut73dq 223 2 3 3 LS cord-329061-1xut73dq 223 3 ) ) -RRB- cord-329061-1xut73dq 223 4 Dr. Dr. NNP cord-329061-1xut73dq 223 5 R. R. NNP cord-329061-1xut73dq 223 6 E. E. NNP cord-329061-1xut73dq 223 7 Shope Shope NNP cord-329061-1xut73dq 223 8 tested test VBD cord-329061-1xut73dq 223 9 118 118 CD cord-329061-1xut73dq 223 10 viral viral JJ cord-329061-1xut73dq 223 11 antigens antigen NNS cord-329061-1xut73dq 223 12 prepared prepare VBN cord-329061-1xut73dq 223 13 from from IN cord-329061-1xut73dq 223 14 infected infected JJ cord-329061-1xut73dq 223 15 mouse mouse NN cord-329061-1xut73dq 223 16 brains brain NNS cord-329061-1xut73dq 223 17 with with IN cord-329061-1xut73dq 223 18 antiserum antiserum NN cord-329061-1xut73dq 223 19 to to IN cord-329061-1xut73dq 223 20 SDA SDA NNP cord-329061-1xut73dq 223 21 virus virus NN cord-329061-1xut73dq 223 22 . . . cord-329061-1xut73dq 224 1 These these DT cord-329061-1xut73dq 224 2 mice mouse NNS cord-329061-1xut73dq 224 3 were be VBD cord-329061-1xut73dq 224 4 from from IN cord-329061-1xut73dq 224 5 the the DT cord-329061-1xut73dq 224 6 same same JJ cord-329061-1xut73dq 224 7 colony colony NN cord-329061-1xut73dq 224 8 used use VBN cord-329061-1xut73dq 224 9 for for IN cord-329061-1xut73dq 224 10 our -PRON- PRP$ cord-329061-1xut73dq 224 11 work work NN cord-329061-1xut73dq 224 12 . . . cord-329061-1xut73dq 225 1 He -PRON- PRP cord-329061-1xut73dq 225 2 did do VBD cord-329061-1xut73dq 225 3 not not RB cord-329061-1xut73dq 225 4 find find VB cord-329061-1xut73dq 225 5 any any DT cord-329061-1xut73dq 225 6 reaction reaction NN cord-329061-1xut73dq 225 7 in in IN cord-329061-1xut73dq 225 8 CF CF NNP cord-329061-1xut73dq 225 9 tests test NNS cord-329061-1xut73dq 225 10 with with IN cord-329061-1xut73dq 225 11 immune immune JJ cord-329061-1xut73dq 225 12 serum serum NN cord-329061-1xut73dq 225 13 to to IN cord-329061-1xut73dq 225 14 SDA SDA NNP cord-329061-1xut73dq 225 15 virus virus NN cord-329061-1xut73dq 225 16 . . . cord-329061-1xut73dq 226 1 ( ( -LRB- cord-329061-1xut73dq 226 2 4 4 LS cord-329061-1xut73dq 226 3 ) ) -RRB- cord-329061-1xut73dq 226 4 Immune Immune NNP cord-329061-1xut73dq 226 5 sera sera NNP cord-329061-1xut73dq 226 6 prepared prepare VBD cord-329061-1xut73dq 226 7 in in IN cord-329061-1xut73dq 226 8 rats rat NNS cord-329061-1xut73dq 226 9 with with IN cord-329061-1xut73dq 226 10 virus virus NN cord-329061-1xut73dq 226 11 passaged passage VBN cord-329061-1xut73dq 226 12 in in IN cord-329061-1xut73dq 226 13 salivary salivary JJ cord-329061-1xut73dq 226 14 glands gland NNS cord-329061-1xut73dq 226 15 reacted react VBD cord-329061-1xut73dq 226 16 with with IN cord-329061-1xut73dq 226 17 antigen antigen NN cord-329061-1xut73dq 226 18 from from IN cord-329061-1xut73dq 226 19 murine murine JJ cord-329061-1xut73dq 226 20 brain brain NN cord-329061-1xut73dq 226 21 , , , cord-329061-1xut73dq 226 22 while while IN cord-329061-1xut73dq 226 23 sera sera NNP cord-329061-1xut73dq 226 24 taken take VBN cord-329061-1xut73dq 226 25 from from IN cord-329061-1xut73dq 226 26 the the DT cord-329061-1xut73dq 226 27 same same JJ cord-329061-1xut73dq 226 28 rats rat NNS cord-329061-1xut73dq 226 29 before before IN cord-329061-1xut73dq 226 30 immunization immunization NN cord-329061-1xut73dq 226 31 did do VBD cord-329061-1xut73dq 226 32 not not RB cord-329061-1xut73dq 226 33 . . . cord-329061-1xut73dq 227 1 ( ( -LRB- cord-329061-1xut73dq 227 2 5 5 LS cord-329061-1xut73dq 227 3 ) ) -RRB- cord-329061-1xut73dq 227 4 SDA SDA NNP cord-329061-1xut73dq 227 5 was be VBD cord-329061-1xut73dq 227 6 reproduced reproduce VBN cord-329061-1xut73dq 227 7 in in IN cord-329061-1xut73dq 227 8 rats rat NNS cord-329061-1xut73dq 227 9 by by IN cord-329061-1xut73dq 227 10 inoculation inoculation NN cord-329061-1xut73dq 227 11 of of IN cord-329061-1xut73dq 227 12 mouse mouse NN cord-329061-1xut73dq 227 13 - - HYPH cord-329061-1xut73dq 227 14 brain brain NN cord-329061-1xut73dq 227 15 - - HYPH cord-329061-1xut73dq 227 16 adapted adapt VBN cord-329061-1xut73dq 227 17 virus virus NN cord-329061-1xut73dq 227 18 . . . cord-329061-1xut73dq 228 1 Failure failure NN cord-329061-1xut73dq 228 2 to to TO cord-329061-1xut73dq 228 3 adapt adapt VB cord-329061-1xut73dq 228 4 SDA SDA NNP cord-329061-1xut73dq 228 5 virus virus NN cord-329061-1xut73dq 228 6 to to IN cord-329061-1xut73dq 228 7 tissue tissue NN cord-329061-1xut73dq 228 8 - - HYPH cord-329061-1xut73dq 228 9 culture culture NN cord-329061-1xut73dq 228 10 systems system NNS cord-329061-1xut73dq 228 11 other other JJ cord-329061-1xut73dq 228 12 than than IN cord-329061-1xut73dq 228 13 primary primary JJ cord-329061-1xut73dq 228 14 rat rat NN cord-329061-1xut73dq 228 15 - - HYPH cord-329061-1xut73dq 228 16 kidney kidney NN cord-329061-1xut73dq 228 17 cultures culture NNS cord-329061-1xut73dq 228 18 confirms confirm VBZ cord-329061-1xut73dq 228 19 the the DT cord-329061-1xut73dq 228 20 observation observation NN cord-329061-1xut73dq 228 21 that that IN cord-329061-1xut73dq 228 22 coronaviruses coronaviruse NNS cord-329061-1xut73dq 228 23 are be VBP cord-329061-1xut73dq 228 24 fastidious fastidious JJ cord-329061-1xut73dq 228 25 in in IN cord-329061-1xut73dq 228 26 their -PRON- PRP$ cord-329061-1xut73dq 228 27 cultural cultural JJ cord-329061-1xut73dq 228 28 requirements requirement NNS cord-329061-1xut73dq 228 29 [ [ -LRB- cord-329061-1xut73dq 228 30 18 18 CD cord-329061-1xut73dq 228 31 ] ] -RRB- cord-329061-1xut73dq 228 32 . . . cord-329061-1xut73dq 229 1 Absence absence NN cord-329061-1xut73dq 229 2 of of IN cord-329061-1xut73dq 229 3 viral viral JJ cord-329061-1xut73dq 229 4 multiplication multiplication NN cord-329061-1xut73dq 229 5 in in IN cord-329061-1xut73dq 229 6 mono1ayers mono1ayers NNPS cord-329061-1xut73dq 229 7 of of IN cord-329061-1xut73dq 229 8 ratsalivary ratsalivary NNP cord-329061-1xut73dq 229 9 - - HYPH cord-329061-1xut73dq 229 10 gland gland NN cord-329061-1xut73dq 229 11 and and CC cord-329061-1xut73dq 229 12 mouse mouse NN cord-329061-1xut73dq 229 13 - - HYPH cord-329061-1xut73dq 229 14 brain brain NN cord-329061-1xut73dq 229 15 tissue tissue NN cord-329061-1xut73dq 229 16 may may MD cord-329061-1xut73dq 229 17 be be VB cord-329061-1xut73dq 229 18 due due JJ cord-329061-1xut73dq 229 19 to to IN cord-329061-1xut73dq 229 20 absence absence NN cord-329061-1xut73dq 229 21 in in IN cord-329061-1xut73dq 229 22 culture culture NN cord-329061-1xut73dq 229 23 of of IN cord-329061-1xut73dq 229 24 cells cell NNS cord-329061-1xut73dq 229 25 that that WDT cord-329061-1xut73dq 229 26 normally normally RB cord-329061-1xut73dq 229 27 support support VBP cord-329061-1xut73dq 229 28 growth growth NN cord-329061-1xut73dq 229 29 of of IN cord-329061-1xut73dq 229 30 virus virus NN cord-329061-1xut73dq 229 31 in in IN cord-329061-1xut73dq 229 32 the the DT cord-329061-1xut73dq 229 33 intact intact JJ cord-329061-1xut73dq 229 34 host host NN cord-329061-1xut73dq 229 35 . . . cord-329061-1xut73dq 230 1 Complement complement NN cord-329061-1xut73dq 230 2 - - HYPH cord-329061-1xut73dq 230 3 fixing fix VBG cord-329061-1xut73dq 230 4 and and CC cord-329061-1xut73dq 230 5 neutralizing neutralize VBG cord-329061-1xut73dq 230 6 antibodies antibody NNS cord-329061-1xut73dq 230 7 to to IN cord-329061-1xut73dq 230 8 MHV MHV NNP cord-329061-1xut73dq 230 9 were be VBD cord-329061-1xut73dq 230 10 detected detect VBN cord-329061-1xut73dq 230 11 in in IN cord-329061-1xut73dq 230 12 the the DT cord-329061-1xut73dq 230 13 sera sera NNP cord-329061-1xut73dq 230 14 of of IN cord-329061-1xut73dq 230 15 men man NNS cord-329061-1xut73dq 230 16 and and CC cord-329061-1xut73dq 230 17 rats rat NNS cord-329061-1xut73dq 230 18 by by IN cord-329061-1xut73dq 230 19 Hartley Hartley NNP cord-329061-1xut73dq 230 20 et et NNP cord-329061-1xut73dq 230 21 al al NNP cord-329061-1xut73dq 230 22 . . . cord-329061-1xut73dq 231 1 [ [ -LRB- cord-329061-1xut73dq 231 2 19 19 CD cord-329061-1xut73dq 231 3 ] ] -RRB- cord-329061-1xut73dq 231 4 and and CC cord-329061-1xut73dq 231 5 were be VBD cord-329061-1xut73dq 231 6 interpreted interpret VBN cord-329061-1xut73dq 231 7 as as IN cord-329061-1xut73dq 231 8 indication indication NN cord-329061-1xut73dq 231 9 of of IN cord-329061-1xut73dq 231 10 infection infection NN cord-329061-1xut73dq 231 11 by by IN cord-329061-1xut73dq 231 12 antigenically antigenically RB cord-329061-1xut73dq 231 13 - - HYPH cord-329061-1xut73dq 231 14 related relate VBN cord-329061-1xut73dq 231 15 , , , cord-329061-1xut73dq 231 16 species species NN cord-329061-1xut73dq 231 17 - - HYPH cord-329061-1xut73dq 231 18 specific specific JJ cord-329061-1xut73dq 231 19 viruses virus NNS cord-329061-1xut73dq 231 20 . . . cord-329061-1xut73dq 232 1 This this DT cord-329061-1xut73dq 232 2 hypothesis hypothesis NN cord-329061-1xut73dq 232 3 has have VBZ cord-329061-1xut73dq 232 4 been be VBN cord-329061-1xut73dq 232 5 confirmed confirm VBN cord-329061-1xut73dq 232 6 in in IN cord-329061-1xut73dq 232 7 part part NN cord-329061-1xut73dq 232 8 by by IN cord-329061-1xut73dq 232 9 isolation isolation NN cord-329061-1xut73dq 232 10 of of IN cord-329061-1xut73dq 232 11 coronaviruses coronaviruse NNS cord-329061-1xut73dq 232 12 from from IN cord-329061-1xut73dq 232 13 man man NN cord-329061-1xut73dq 232 14 [ [ -LRB- cord-329061-1xut73dq 232 15 18 18 CD cord-329061-1xut73dq 232 16 ] ] -RRB- cord-329061-1xut73dq 232 17 . . . cord-329061-1xut73dq 233 1 SDA SDA NNP cord-329061-1xut73dq 233 2 agent agent NN cord-329061-1xut73dq 233 3 and and CC cord-329061-1xut73dq 233 4 Parker Parker NNP cord-329061-1xut73dq 233 5 's 's POS cord-329061-1xut73dq 233 6 rat rat NNP cord-329061-1xut73dq 233 7 corona corona NN cord-329061-1xut73dq 233 8 agent agent NN cord-329061-1xut73dq 233 9 may may MD cord-329061-1xut73dq 233 10 be be VB cord-329061-1xut73dq 233 11 responsible responsible JJ cord-329061-1xut73dq 233 12 for for IN cord-329061-1xut73dq 233 13 antibodies antibody NNS cord-329061-1xut73dq 233 14 to to IN cord-329061-1xut73dq 233 15 MHV MHV NNP cord-329061-1xut73dq 233 16 found find VBN cord-329061-1xut73dq 233 17 in in IN cord-329061-1xut73dq 233 18 rats rat NNS cord-329061-1xut73dq 233 19 . . . cord-329061-1xut73dq 234 1 Biologically biologically RB cord-329061-1xut73dq 234 2 , , , cord-329061-1xut73dq 234 3 SDA SDA NNP cord-329061-1xut73dq 234 4 virus virus NN cord-329061-1xut73dq 234 5 behaves behave VBZ cord-329061-1xut73dq 234 6 differently differently RB cord-329061-1xut73dq 234 7 from from IN cord-329061-1xut73dq 234 8 known know VBN cord-329061-1xut73dq 234 9 strains strain NNS cord-329061-1xut73dq 234 10 of of IN cord-329061-1xut73dq 234 11 MHV MHV NNP cord-329061-1xut73dq 234 12 , , , cord-329061-1xut73dq 234 13 since since IN cord-329061-1xut73dq 234 14 the the DT cord-329061-1xut73dq 234 15 former former JJ cord-329061-1xut73dq 234 16 does do VBZ cord-329061-1xut73dq 234 17 not not RB cord-329061-1xut73dq 234 18 multiply multiply VB cord-329061-1xut73dq 234 19 in in IN cord-329061-1xut73dq 234 20 the the DT cord-329061-1xut73dq 234 21 Py Py NNP cord-329061-1xut73dq 234 22 - - HYPH cord-329061-1xut73dq 234 23 AL/ AL/ NNP cord-329061-1xut73dq 234 24 N n CD cord-329061-1xut73dq 234 25 cell cell NN cord-329061-1xut73dq 234 26 line line NN cord-329061-1xut73dq 234 27 , , , cord-329061-1xut73dq 234 28 which which WDT cord-329061-1xut73dq 234 29 is be VBZ cord-329061-1xut73dq 234 30 highly highly RB cord-329061-1xut73dq 234 31 susceptible susceptible JJ cord-329061-1xut73dq 234 32 to to IN cord-329061-1xut73dq 234 33 MHV MHV NNP cord-329061-1xut73dq 234 34 [ [ -LRB- cord-329061-1xut73dq 234 35 6 6 CD cord-329061-1xut73dq 234 36 ] ] -RRB- cord-329061-1xut73dq 234 37 . . . cord-329061-1xut73dq 235 1 In in IN cord-329061-1xut73dq 235 2 addition addition NN cord-329061-1xut73dq 235 3 , , , cord-329061-1xut73dq 235 4 SDA SDA NNP cord-329061-1xut73dq 235 5 virus virus NN cord-329061-1xut73dq 235 6 does do VBZ cord-329061-1xut73dq 235 7 not not RB cord-329061-1xut73dq 235 8 induce induce VB cord-329061-1xut73dq 235 9 lesions lesion NNS cord-329061-1xut73dq 235 10 compatible compatible JJ cord-329061-1xut73dq 235 11 with with IN cord-329061-1xut73dq 235 12 those those DT cord-329061-1xut73dq 235 13 due due JJ cord-329061-1xut73dq 235 14 to to IN cord-329061-1xut73dq 235 15 MHV MHV NNP cord-329061-1xut73dq 235 16 if if IN cord-329061-1xut73dq 235 17 inoculated inoculate VBN cord-329061-1xut73dq 235 18 ic ic NNP cord-329061-1xut73dq 235 19 into into IN cord-329061-1xut73dq 235 20 infant infant JJ cord-329061-1xut73dq 235 21 mice mouse NNS cord-329061-1xut73dq 235 22 or or CC cord-329061-1xut73dq 235 23 if if IN cord-329061-1xut73dq 235 24 inoculated inoculate VBN cord-329061-1xut73dq 235 25 ic ic NNP cord-329061-1xut73dq 235 26 or or CC cord-329061-1xut73dq 235 27 ip ip NN cord-329061-1xut73dq 235 28 into into IN cord-329061-1xut73dq 235 29 cortisone cortisone NN cord-329061-1xut73dq 235 30 - - HYPH cord-329061-1xut73dq 235 31 treated treat VBN cord-329061-1xut73dq 235 32 weanling weanle VBG cord-329061-1xut73dq 235 33 mice mouse NNS cord-329061-1xut73dq 235 34 . . . cord-329061-1xut73dq 236 1 Therefore therefore RB cord-329061-1xut73dq 236 2 , , , cord-329061-1xut73dq 236 3 our -PRON- PRP$ cord-329061-1xut73dq 236 4 failure failure NN cord-329061-1xut73dq 236 5 to to TO cord-329061-1xut73dq 236 6 detect detect VB cord-329061-1xut73dq 236 7 antigenic antigenic JJ cord-329061-1xut73dq 236 8 differences difference NNS cord-329061-1xut73dq 236 9 between between IN cord-329061-1xut73dq 236 10 these these DT cord-329061-1xut73dq 236 11 agents agent NNS cord-329061-1xut73dq 236 12 by by IN cord-329061-1xut73dq 236 13 the the DT cord-329061-1xut73dq 236 14 complement complement NN cord-329061-1xut73dq 236 15 - - HYPH cord-329061-1xut73dq 236 16 fixation fixation NN cord-329061-1xut73dq 236 17 test test NN cord-329061-1xut73dq 236 18 should should MD cord-329061-1xut73dq 236 19 be be VB cord-329061-1xut73dq 236 20 interpreted interpret VBN cord-329061-1xut73dq 236 21 with with IN cord-329061-1xut73dq 236 22 caution caution NN cord-329061-1xut73dq 236 23 . . . cord-329061-1xut73dq 237 1 More More JJR cord-329061-1xut73dq 237 2 information information NN cord-329061-1xut73dq 237 3 may may MD cord-329061-1xut73dq 237 4 be be VB cord-329061-1xut73dq 237 5 obtained obtain VBN cord-329061-1xut73dq 237 6 for for IN cord-329061-1xut73dq 237 7 serologic serologic JJ cord-329061-1xut73dq 237 8 differentiation differentiation NN cord-329061-1xut73dq 237 9 by by IN cord-329061-1xut73dq 237 10 use use NN cord-329061-1xut73dq 237 11 of of IN cord-329061-1xut73dq 237 12 immune immune JJ cord-329061-1xut73dq 237 13 sera sera NNP cord-329061-1xut73dq 237 14 prepared prepare VBN cord-329061-1xut73dq 237 15 by by IN cord-329061-1xut73dq 237 16 different different JJ cord-329061-1xut73dq 237 17 schedules schedule NNS cord-329061-1xut73dq 237 18 of of IN cord-329061-1xut73dq 237 19 immunization immunization NN cord-329061-1xut73dq 237 20 and and CC cord-329061-1xut73dq 237 21 then then RB cord-329061-1xut73dq 237 22 tested test VBN cord-329061-1xut73dq 237 23 by by IN cord-329061-1xut73dq 237 24 neutralization neutralization NN cord-329061-1xut73dq 237 25 , , , cord-329061-1xut73dq 237 26 complement complement NN cord-329061-1xut73dq 237 27 - - HYPH cord-329061-1xut73dq 237 28 fixation fixation NN cord-329061-1xut73dq 237 29 , , , cord-329061-1xut73dq 237 30 fluorescent fluorescent NN cord-329061-1xut73dq 237 31 - - HYPH cord-329061-1xut73dq 237 32 antibody antibody NN cord-329061-1xut73dq 237 33 , , , cord-329061-1xut73dq 237 34 and and CC cord-329061-1xut73dq 237 35 gel gel NN cord-329061-1xut73dq 237 36 - - HYPH cord-329061-1xut73dq 237 37 diffusion diffusion NN cord-329061-1xut73dq 237 38 methods method NNS cord-329061-1xut73dq 237 39 . . . cord-329061-1xut73dq 238 1 Since since IN cord-329061-1xut73dq 238 2 there there EX cord-329061-1xut73dq 238 3 is be VBZ cord-329061-1xut73dq 238 4 more more JJR cord-329061-1xut73dq 238 5 than than IN cord-329061-1xut73dq 238 6 one one CD cord-329061-1xut73dq 238 7 serotype serotype NN cord-329061-1xut73dq 238 8 of of IN cord-329061-1xut73dq 238 9 human human NN cord-329061-1xut73dq 238 10 and and CC cord-329061-1xut73dq 238 11 mouse mouse NN cord-329061-1xut73dq 238 12 coronavirus coronavirus NN cord-329061-1xut73dq 238 13 [ [ -LRB- cord-329061-1xut73dq 238 14 18 18 CD cord-329061-1xut73dq 238 15 , , , cord-329061-1xut73dq 238 16 20 20 CD cord-329061-1xut73dq 238 17 ] ] -RRB- cord-329061-1xut73dq 238 18 , , , cord-329061-1xut73dq 238 19 it -PRON- PRP cord-329061-1xut73dq 238 20 is be VBZ cord-329061-1xut73dq 238 21 not not RB cord-329061-1xut73dq 238 22 surprising surprising JJ cord-329061-1xut73dq 238 23 that that IN cord-329061-1xut73dq 238 24 , , , cord-329061-1xut73dq 238 25 although although IN cord-329061-1xut73dq 238 26 there there EX cord-329061-1xut73dq 238 27 is be VBZ cord-329061-1xut73dq 238 28 much much JJ cord-329061-1xut73dq 238 29 crossneutralization crossneutralization NN cord-329061-1xut73dq 238 30 , , , cord-329061-1xut73dq 238 31 there there EX cord-329061-1xut73dq 238 32 seem seem VBP cord-329061-1xut73dq 238 33 to to TO cord-329061-1xut73dq 238 34 be be VB cord-329061-1xut73dq 238 35 antigenic antigenic JJ cord-329061-1xut73dq 238 36 differences difference NNS cord-329061-1xut73dq 238 37 between between IN cord-329061-1xut73dq 238 38 our -PRON- PRP$ cord-329061-1xut73dq 238 39 virus virus NN cord-329061-1xut73dq 238 40 and and CC cord-329061-1xut73dq 238 41 the the DT cord-329061-1xut73dq 238 42 one one NN cord-329061-1xut73dq 238 43 isolated isolate VBN cord-329061-1xut73dq 238 44 by by IN cord-329061-1xut73dq 238 45 Parker Parker NNP cord-329061-1xut73dq 238 46 et et NNP cord-329061-1xut73dq 238 47 al al NNP cord-329061-1xut73dq 238 48 . . . cord-329061-1xut73dq 239 1 [ [ -LRB- cord-329061-1xut73dq 239 2 4 4 CD cord-329061-1xut73dq 239 3 ] ] -RRB- cord-329061-1xut73dq 239 4 . . . cord-329061-1xut73dq 240 1 Several several JJ cord-329061-1xut73dq 240 2 serotypes serotype NNS cord-329061-1xut73dq 240 3 of of IN cord-329061-1xut73dq 240 4 rat rat NN cord-329061-1xut73dq 240 5 coronaviruses coronaviruse NNS cord-329061-1xut73dq 240 6 may may MD cord-329061-1xut73dq 240 7 therefore therefore RB cord-329061-1xut73dq 240 8 exist exist VB cord-329061-1xut73dq 240 9 . . . cord-329061-1xut73dq 241 1 It -PRON- PRP cord-329061-1xut73dq 241 2 is be VBZ cord-329061-1xut73dq 241 3 necessary necessary JJ cord-329061-1xut73dq 241 4 to to TO cord-329061-1xut73dq 241 5 delineate delineate VB cord-329061-1xut73dq 241 6 further further RB cord-329061-1xut73dq 241 7 the the DT cord-329061-1xut73dq 241 8 serotypes serotype NNS cord-329061-1xut73dq 241 9 , , , cord-329061-1xut73dq 241 10 the the DT cord-329061-1xut73dq 241 11 disease disease NN cord-329061-1xut73dq 241 12 poten poten NNP cord-329061-1xut73dq 241 13 - - HYPH cord-329061-1xut73dq 241 14 tials tial NNS cord-329061-1xut73dq 241 15 , , , cord-329061-1xut73dq 241 16 and and CC cord-329061-1xut73dq 241 17 the the DT cord-329061-1xut73dq 241 18 epidemiology epidemiology NN cord-329061-1xut73dq 241 19 of of IN cord-329061-1xut73dq 241 20 both both DT cord-329061-1xut73dq 241 21 agents agent NNS cord-329061-1xut73dq 241 22 . . . cord-329061-1xut73dq 242 1 For for IN cord-329061-1xut73dq 242 2 example example NN cord-329061-1xut73dq 242 3 , , , cord-329061-1xut73dq 242 4 our -PRON- PRP$ cord-329061-1xut73dq 242 5 agent agent NN cord-329061-1xut73dq 242 6 is be VBZ cord-329061-1xut73dq 242 7 known know VBN cord-329061-1xut73dq 242 8 to to TO cord-329061-1xut73dq 242 9 cause cause VB cord-329061-1xut73dq 242 10 SDA SDA NNP cord-329061-1xut73dq 242 11 , , , cord-329061-1xut73dq 242 12 while while IN cord-329061-1xut73dq 242 13 Parker Parker NNP cord-329061-1xut73dq 242 14 's 's POS cord-329061-1xut73dq 242 15 rat rat NN cord-329061-1xut73dq 242 16 coronavirus coronavirus NN cord-329061-1xut73dq 242 17 causes cause VBZ cord-329061-1xut73dq 242 18 pulmonary pulmonary JJ cord-329061-1xut73dq 242 19 lesions lesion NNS cord-329061-1xut73dq 242 20 . . . cord-329061-1xut73dq 243 1 The the DT cord-329061-1xut73dq 243 2 ability ability NN cord-329061-1xut73dq 243 3 of of IN cord-329061-1xut73dq 243 4 mouse mouse NN cord-329061-1xut73dq 243 5 - - HYPH cord-329061-1xut73dq 243 6 brain brain NN cord-329061-1xut73dq 243 7 - - HYPH cord-329061-1xut73dq 243 8 adapted adapt VBN cord-329061-1xut73dq 243 9 virus virus NN cord-329061-1xut73dq 243 10 to to TO cord-329061-1xut73dq 243 11 produce produce VB cord-329061-1xut73dq 243 12 the the DT cord-329061-1xut73dq 243 13 disease disease NN cord-329061-1xut73dq 243 14 in in IN cord-329061-1xut73dq 243 15 rats rat NNS cord-329061-1xut73dq 243 16 indicates indicate VBZ cord-329061-1xut73dq 243 17 that that IN cord-329061-1xut73dq 243 18 we -PRON- PRP cord-329061-1xut73dq 243 19 are be VBP cord-329061-1xut73dq 243 20 dealing deal VBG cord-329061-1xut73dq 243 21 with with IN cord-329061-1xut73dq 243 22 the the DT cord-329061-1xut73dq 243 23 same same JJ cord-329061-1xut73dq 243 24 agent agent NN cord-329061-1xut73dq 243 25 . . . cord-329061-1xut73dq 244 1 An an DT cord-329061-1xut73dq 244 2 evaluation evaluation NN cord-329061-1xut73dq 244 3 of of IN cord-329061-1xut73dq 244 4 the the DT cord-329061-1xut73dq 244 5 glands gland NNS cord-329061-1xut73dq 244 6 involved involve VBN cord-329061-1xut73dq 244 7 and and CC cord-329061-1xut73dq 244 8 the the DT cord-329061-1xut73dq 244 9 extent extent NN cord-329061-1xut73dq 244 10 of of IN cord-329061-1xut73dq 244 11 involvement involvement NN cord-329061-1xut73dq 244 12 may may MD cord-329061-1xut73dq 244 13 indicate indicate VB cord-329061-1xut73dq 244 14 a a DT cord-329061-1xut73dq 244 15 shift shift NN cord-329061-1xut73dq 244 16 in in IN cord-329061-1xut73dq 244 17 tissue tissue NN cord-329061-1xut73dq 244 18 tropism tropism NN cord-329061-1xut73dq 244 19 and and CC cord-329061-1xut73dq 244 20 also also RB cord-329061-1xut73dq 244 21 an an DT cord-329061-1xut73dq 244 22 apparent apparent JJ cord-329061-1xut73dq 244 23 reduction reduction NN cord-329061-1xut73dq 244 24 in in IN cord-329061-1xut73dq 244 25 virulence virulence NN cord-329061-1xut73dq 244 26 of of IN cord-329061-1xut73dq 244 27 virus virus NN cord-329061-1xut73dq 244 28 . . . cord-329061-1xut73dq 245 1 SDA SDA NNP cord-329061-1xut73dq 245 2 virus virus NN cord-329061-1xut73dq 245 3 was be VBD cord-329061-1xut73dq 245 4 not not RB cord-329061-1xut73dq 245 5 recovered recover VBN cord-329061-1xut73dq 245 6 from from IN cord-329061-1xut73dq 245 7 submaxillary submaxillary JJ cord-329061-1xut73dq 245 8 salivary salivary NN cord-329061-1xut73dq 245 9 glands gland NNS cord-329061-1xut73dq 245 10 when when WRB cord-329061-1xut73dq 245 11 mouse mouse NN cord-329061-1xut73dq 245 12 - - HYPH cord-329061-1xut73dq 245 13 adapted adapt VBN cord-329061-1xut73dq 245 14 - - HYPH cord-329061-1xut73dq 245 15 virus virus NN cord-329061-1xut73dq 245 16 was be VBD cord-329061-1xut73dq 245 17 inoculated inoculate VBN cord-329061-1xut73dq 245 18 into into IN cord-329061-1xut73dq 245 19 rats rat NNS cord-329061-1xut73dq 245 20 , , , cord-329061-1xut73dq 245 21 but but CC cord-329061-1xut73dq 245 22 a a DT cord-329061-1xut73dq 245 23 change change NN cord-329061-1xut73dq 245 24 in in IN cord-329061-1xut73dq 245 25 tissue tissue NN cord-329061-1xut73dq 245 26 tropism tropism NN cord-329061-1xut73dq 245 27 may may MD cord-329061-1xut73dq 245 28 have have VB cord-329061-1xut73dq 245 29 accounted account VBN cord-329061-1xut73dq 245 30 for for IN cord-329061-1xut73dq 245 31 this this DT cord-329061-1xut73dq 245 32 difficulty difficulty NN cord-329061-1xut73dq 245 33 . . . cord-329061-1xut73dq 246 1 Attempts attempt NNS cord-329061-1xut73dq 246 2 were be VBD cord-329061-1xut73dq 246 3 not not RB cord-329061-1xut73dq 246 4 made make VBN cord-329061-1xut73dq 246 5 to to TO cord-329061-1xut73dq 246 6 isolate isolate VB cord-329061-1xut73dq 246 7 virus virus NN cord-329061-1xut73dq 246 8 from from IN cord-329061-1xut73dq 246 9 parotid parotid JJ cord-329061-1xut73dq 246 10 and and CC cord-329061-1xut73dq 246 11 exorbital exorbital JJ cord-329061-1xut73dq 246 12 glands gland NNS cord-329061-1xut73dq 246 13 , , , cord-329061-1xut73dq 246 14 but but CC cord-329061-1xut73dq 246 15 histopathologically histopathologically RB cord-329061-1xut73dq 246 16 they -PRON- PRP cord-329061-1xut73dq 246 17 demonstrated demonstrate VBD cord-329061-1xut73dq 246 18 classical classical JJ cord-329061-1xut73dq 246 19 lesions lesion NNS cord-329061-1xut73dq 246 20 . . . cord-329061-1xut73dq 247 1 These these DT cord-329061-1xut73dq 247 2 tissues tissue NNS cord-329061-1xut73dq 247 3 were be VBD cord-329061-1xut73dq 247 4 only only RB cord-329061-1xut73dq 247 5 minimally minimally RB cord-329061-1xut73dq 247 6 involved involved JJ cord-329061-1xut73dq 247 7 in in IN cord-329061-1xut73dq 247 8 previous previous JJ cord-329061-1xut73dq 247 9 studies study NNS cord-329061-1xut73dq 247 10 with with IN cord-329061-1xut73dq 247 11 the the DT cord-329061-1xut73dq 247 12 strain strain NN cord-329061-1xut73dq 247 13 passaged passage VBN cord-329061-1xut73dq 247 14 in in IN cord-329061-1xut73dq 247 15 rats rat NNS cord-329061-1xut73dq 247 16 . . . cord-329061-1xut73dq 248 1 The the DT cord-329061-1xut73dq 248 2 apparent apparent JJ cord-329061-1xut73dq 248 3 shift shift NN cord-329061-1xut73dq 248 4 in in IN cord-329061-1xut73dq 248 5 tissue tissue NN cord-329061-1xut73dq 248 6 tropism tropism NN cord-329061-1xut73dq 248 7 needs need VBZ cord-329061-1xut73dq 248 8 confirmation confirmation NN cord-329061-1xut73dq 248 9 in in IN cord-329061-1xut73dq 248 10 both both CC cord-329061-1xut73dq 248 11 germfree germfree JJ cord-329061-1xut73dq 248 12 and and CC cord-329061-1xut73dq 248 13 conventional conventional JJ cord-329061-1xut73dq 248 14 , , , cord-329061-1xut73dq 248 15 susceptible susceptible JJ cord-329061-1xut73dq 248 16 rats rat NNS cord-329061-1xut73dq 248 17 of of IN cord-329061-1xut73dq 248 18 the the DT cord-329061-1xut73dq 248 19 same same JJ cord-329061-1xut73dq 248 20 strain strain NN cord-329061-1xut73dq 248 21 , , , cord-329061-1xut73dq 248 22 particularly particularly RB cord-329061-1xut73dq 248 23 since since IN cord-329061-1xut73dq 248 24 the the DT cord-329061-1xut73dq 248 25 transmission transmission NN cord-329061-1xut73dq 248 26 experiments experiment NNS cord-329061-1xut73dq 248 27 were be VBD cord-329061-1xut73dq 248 28 performed perform VBN cord-329061-1xut73dq 248 29 in in IN cord-329061-1xut73dq 248 30 susceptible susceptible JJ cord-329061-1xut73dq 248 31 , , , cord-329061-1xut73dq 248 32 specific specific JJ cord-329061-1xut73dq 248 33 - - HYPH cord-329061-1xut73dq 248 34 pathogenfree pathogenfree JJ cord-329061-1xut73dq 248 35 Wistar Wistar NNP cord-329061-1xut73dq 248 36 rats rat NNS cord-329061-1xut73dq 248 37 . . . cord-329061-1xut73dq 249 1 Sialodacryoadenitis Sialodacryoadenitis NNP cord-329061-1xut73dq 249 2 in in IN cord-329061-1xut73dq 249 3 the the DT cord-329061-1xut73dq 249 4 rat rat NN cord-329061-1xut73dq 249 5 . . . cord-329061-1xut73dq 250 1 A a DT cord-329061-1xut73dq 250 2 light light NN cord-329061-1xut73dq 250 3 and and CC cord-329061-1xut73dq 250 4 electron electron NN cord-329061-1xut73dq 250 5 microscopic microscopic JJ cord-329061-1xut73dq 250 6 study study NN cord-329061-1xut73dq 250 7 Studies study NNS cord-329061-1xut73dq 250 8 on on IN cord-329061-1xut73dq 250 9 the the DT cord-329061-1xut73dq 250 10 agent agent NN cord-329061-1xut73dq 250 11 of of IN cord-329061-1xut73dq 250 12 sialodacryoadenitis sialodacryoadenitis NNP cord-329061-1xut73dq 250 13 of of IN cord-329061-1xut73dq 250 14 rats rat NNS cord-329061-1xut73dq 250 15 Proc proc VB cord-329061-1xut73dq 250 16 . . . cord-329061-1xut73dq 251 1 p p NNP cord-329061-1xut73dq 251 2 Rat rat NN cord-329061-1xut73dq 251 3 coronavirus coronavirus NN cord-329061-1xut73dq 251 4 ( ( -LRB- cord-329061-1xut73dq 251 5 RCV RCV NNP cord-329061-1xut73dq 251 6 ) ) -RRB- cord-329061-1xut73dq 251 7 : : : cord-329061-1xut73dq 251 8 a a DT cord-329061-1xut73dq 251 9 prevalent prevalent JJ cord-329061-1xut73dq 251 10 , , , cord-329061-1xut73dq 251 11 naturally naturally RB cord-329061-1xut73dq 251 12 occurring occur VBG cord-329061-1xut73dq 251 13 pneumotropic pneumotropic NNP cord-329061-1xut73dq 251 14 virus virus NN cord-329061-1xut73dq 251 15 of of IN cord-329061-1xut73dq 251 16 rats rat NNS cord-329061-1xut73dq 251 17 . . . cord-329061-1xut73dq 252 1 Arch Arch NNP cord-329061-1xut73dq 252 2 . . . cord-329061-1xut73dq 253 1 Gesamte Gesamte NNP cord-329061-1xut73dq 253 2 Virusforsch Virusforsch NNP cord-329061-1xut73dq 253 3 Fluorescent Fluorescent NNP cord-329061-1xut73dq 253 4 antibody antibody NN cord-329061-1xut73dq 253 5 methods method NNS cord-329061-1xut73dq 254 1 Enhanced enhanced JJ cord-329061-1xut73dq 254 2 growth growth NN cord-329061-1xut73dq 254 3 of of IN cord-329061-1xut73dq 254 4 murine murine JJ cord-329061-1xut73dq 254 5 corona corona NN cord-329061-1xut73dq 254 6 viruses virus NNS cord-329061-1xut73dq 254 7 in in IN cord-329061-1xut73dq 254 8 cells cell NNS cord-329061-1xut73dq 254 9 transformed transform VBN cord-329061-1xut73dq 254 10 by by IN cord-329061-1xut73dq 254 11 polyoma polyoma NN cord-329061-1xut73dq 254 12 virus virus NN cord-329061-1xut73dq 254 13 Proc proc NN cord-329061-1xut73dq 254 14 . . . cord-329061-1xut73dq 255 1 p p LS cord-329061-1xut73dq 255 2 Tissue tissue NN cord-329061-1xut73dq 255 3 culture culture NN cord-329061-1xut73dq 255 4 technics technic NNS cord-329061-1xut73dq 255 5 for for IN cord-329061-1xut73dq 255 6 diagnostic diagnostic JJ cord-329061-1xut73dq 255 7 virology virology NN cord-329061-1xut73dq 255 8 Chandipura Chandipura NNP cord-329061-1xut73dq 255 9 , , , cord-329061-1xut73dq 255 10 a a DT cord-329061-1xut73dq 255 11 new new JJ cord-329061-1xut73dq 255 12 arbovirus arbovirus NN cord-329061-1xut73dq 255 13 isolated isolate VBN cord-329061-1xut73dq 255 14 in in IN cord-329061-1xut73dq 255 15 India India NNP cord-329061-1xut73dq 255 16 from from IN cord-329061-1xut73dq 255 17 patients patient NNS cord-329061-1xut73dq 255 18 with with IN cord-329061-1xut73dq 255 19 febrile febrile JJ cord-329061-1xut73dq 255 20 illness illness NN cord-329061-1xut73dq 255 21 Isolation isolation NN cord-329061-1xut73dq 255 22 and and CC cord-329061-1xut73dq 255 23 characterization characterization NN cord-329061-1xut73dq 255 24 of of IN cord-329061-1xut73dq 255 25 a a DT cord-329061-1xut73dq 255 26 herpeslike herpeslike NN cord-329061-1xut73dq 256 1 ( ( -LRB- cord-329061-1xut73dq 256 2 Hsiung Hsiung NNP cord-329061-1xut73dq 256 3 - - HYPH cord-329061-1xut73dq 256 4 Kaplow Kaplow NNP cord-329061-1xut73dq 256 5 ) ) -RRB- cord-329061-1xut73dq 256 6 virus virus NN cord-329061-1xut73dq 256 7 from from IN cord-329061-1xut73dq 256 8 guinea guinea NN cord-329061-1xut73dq 256 9 pigs pig NNS cord-329061-1xut73dq 256 10 Herpes Herpes NNP cord-329061-1xut73dq 256 11 and and CC cord-329061-1xut73dq 256 12 pox pox NN cord-329061-1xut73dq 256 13 viruses virus NNS cord-329061-1xut73dq 256 14 : : : cord-329061-1xut73dq 256 15 laboratory laboratory NN cord-329061-1xut73dq 256 16 procedures procedure NNS cord-329061-1xut73dq 256 17 Application Application NNP cord-329061-1xut73dq 256 18 of of IN cord-329061-1xut73dq 256 19 a a DT cord-329061-1xut73dq 256 20 microtechnique microtechnique NN cord-329061-1xut73dq 256 21 to to IN cord-329061-1xut73dq 256 22 viral viral JJ cord-329061-1xut73dq 256 23 serological serological JJ cord-329061-1xut73dq 256 24 investigations investigation NNS cord-329061-1xut73dq 257 1 A a DT cord-329061-1xut73dq 257 2 simple simple JJ cord-329061-1xut73dq 257 3 method method NN cord-329061-1xut73dq 257 4 of of IN cord-329061-1xut73dq 257 5 estimating estimate VBG cord-329061-1xut73dq 257 6 fifty fifty CD cord-329061-1xut73dq 257 7 per per IN cord-329061-1xut73dq 257 8 cent cent NN cord-329061-1xut73dq 257 9 endpoints endpoint NNS cord-329061-1xut73dq 257 10 Studies Studies NNPS cord-329061-1xut73dq 257 11 of of IN cord-329061-1xut73dq 257 12 a a DT cord-329061-1xut73dq 257 13 myxovirus myxovirus NNP cord-329061-1xut73dq 257 14 recovered recover VBN cord-329061-1xut73dq 257 15 from from IN cord-329061-1xut73dq 257 16 patients patient NNS cord-329061-1xut73dq 257 17 with with IN cord-329061-1xut73dq 257 18 infectious infectious JJ cord-329061-1xut73dq 257 19 hepatitis hepatitis NN cord-329061-1xut73dq 257 20 . . . cord-329061-1xut73dq 258 1 I. I. NNP cord-329061-1xut73dq 258 2 Isolation isolation NN cord-329061-1xut73dq 258 3 and and CC cord-329061-1xut73dq 258 4 characterization characterization NN cord-329061-1xut73dq 258 5 . . . cord-329061-1xut73dq 259 1 1 1 CD cord-329061-1xut73dq 259 2 Ultrafiltration Ultrafiltration NNP cord-329061-1xut73dq 259 3 of of IN cord-329061-1xut73dq 259 4 simian simian JJ cord-329061-1xut73dq 259 5 viruses virus NNS cord-329061-1xut73dq 259 6 Casals Casals NNP cord-329061-1xut73dq 259 7 , , , cord-329061-1xut73dq 259 8 1 1 CD cord-329061-1xut73dq 259 9 . . . cord-329061-1xut73dq 260 1 Filtration filtration NN cord-329061-1xut73dq 260 2 of of IN cord-329061-1xut73dq 260 3 arboviruses arbovirus NNS cord-329061-1xut73dq 260 4 through through IN cord-329061-1xut73dq 260 5 " " `` cord-329061-1xut73dq 260 6 Millipore Millipore NNP cord-329061-1xut73dq 260 7 " " '' cord-329061-1xut73dq 260 8 membranes membrane NNS cord-329061-1xut73dq 260 9 Previously previously RB cord-329061-1xut73dq 260 10 unrecognized unrecognized JJ cord-329061-1xut73dq 260 11 virus virus NN cord-329061-1xut73dq 260 12 from from IN cord-329061-1xut73dq 260 13 submaxillary submaxillary JJ cord-329061-1xut73dq 260 14 glands gland NNS cord-329061-1xut73dq 260 15 of of IN cord-329061-1xut73dq 260 16 gnotobiotic gnotobiotic JJ cord-329061-1xut73dq 260 17 and and CC cord-329061-1xut73dq 260 18 conventional conventional JJ cord-329061-1xut73dq 260 19 rats rat NNS cord-329061-1xut73dq 260 20 Diseases disease NNS cord-329061-1xut73dq 260 21 of of IN cord-329061-1xut73dq 260 22 swine swine NNP cord-329061-1xut73dq 260 23 Corona Corona NNP cord-329061-1xut73dq 260 24 viruses virus NNS cord-329061-1xut73dq 260 25 of of IN cord-329061-1xut73dq 260 26 man man NN cord-329061-1xut73dq 260 27 Antibodies Antibodies NNP cord-329061-1xut73dq 260 28 to to TO cord-329061-1xut73dq 260 29 mouse mouse VB cord-329061-1xut73dq 260 30 hepatitis hepatitis NN cord-329061-1xut73dq 260 31 viruses virus NNS cord-329061-1xut73dq 260 32 in in IN cord-329061-1xut73dq 260 33 human human JJ cord-329061-1xut73dq 260 34 sera sera FW cord-329061-1xut73dq 260 35 Viruses virus NNS cord-329061-1xut73dq 260 36 of of IN cord-329061-1xut73dq 260 37 laboratory laboratory NN cord-329061-1xut73dq 260 38 rodents rodent NNS cord-329061-1xut73dq 260 39 . . . cord-329061-1xut73dq 261 1 National National NNP cord-329061-1xut73dq 261 2 Cancer Cancer NNP cord-329061-1xut73dq 261 3 Institute Institute NNP cord-329061-1xut73dq 261 4 monograph monograph NN cord-329061-1xut73dq 261 5 no no DT