id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329061-1xut73dq	Bhatt, Pravin N.	Characterization of the Virus of Sialodacryoadenitis of Rats: A Member of the Coronavirus Group	1972-08-17		.txt	text/plain	4294	286	59	The virus that causes sialodacryoadenitis in rats has been isolated in mice and in primary cultures of rat-kidney cells and has been characterized as a heat-labile RNA virus that is sensitive to lipid solvents and is relatively stable at pH 3.0. Rats were inoculated intranasally with 0.1 ml of virus-infected salivary-gland suspension, observed daily for evidence of overt illness, and sacrificed at various intervals. Monolayers obtained from explant cultures of submaxillary, parotid, Harderian, and exorbital glands of germfree rats, monolayers of trypsin-dispersed brain cells of infant mice, and a line of polyoma-transformed mouse cells (Py-AL/N) [6J were also tested. The neutralization (N) test with sera immune to murine viruses was performed in infant mice, and these animals were observed for 14 days after inoculation. Infectious virus or viral antigen was not detected when tissue-culture fluids from infected-mouse-brain and Py-AL/N cultures were inoculated ic into infant mice or when monolayers were examined by indirect immunofluorescence. E. Shope tested 118 viral antigens prepared from infected mouse brains with antiserum to SDA virus.	./cache/cord-329061-1xut73dq.txt	./txt/cord-329061-1xut73dq.txt