id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004416-qw6tusd2	Krishna, Smriti M.	Development of a two-stage limb ischemia model to better simulate human peripheral artery disease	2020-02-26		.txt	text/plain	8149	464	49	HLI was more severe in mice receiving the 2-stage compared to the 1-stage ischemia induction procedure as assessed by LDPI (p = 0.014), and reflected in a higher ischemic score (p = 0.004) and lower average distance travelled on a treadmill test (p = 0.045). Mice undergoing the 2-stage HLI also had lower expression of angiogenesis markers (vascular endothelial growth factor, p = 0.004; vascular endothelial growth factorreceptor 2, p = 0.008) and shear stress response mechano-transducer transient receptor potential vanilloid 4 (p = 0.041) within gastrocnemius muscle samples, compared to animals having the 1-stage HLI procedure. In contrast, the most commonly used animal model for initial testing of novel therapies for PAD is a model of acute blood supply interruption through ligation or excision of the femoral artery (referred to here as the 1-stage hind limb ischemia (HLI) model) 14, 15 .	./cache/cord-004416-qw6tusd2.txt	./txt/cord-004416-qw6tusd2.txt