id sid tid token lemma pos cord-293913-frkb8iso 1 1 key key JJ cord-293913-frkb8iso 1 2 : : : cord-293913-frkb8iso 1 3 cord-293913-frkb8iso cord-293913-frkb8iso ADD cord-293913-frkb8iso 1 4 authors author NNS cord-293913-frkb8iso 1 5 : : : cord-293913-frkb8iso 1 6 Gao Gao NNP cord-293913-frkb8iso 1 7 , , , cord-293913-frkb8iso 1 8 Hong Hong NNP cord-293913-frkb8iso 1 9 - - HYPH cord-293913-frkb8iso 1 10 Qiang Qiang NNP cord-293913-frkb8iso 1 11 ; ; : cord-293913-frkb8iso 1 12 Schiller Schiller NNP cord-293913-frkb8iso 1 13 , , , cord-293913-frkb8iso 1 14 Jennifer Jennifer NNP cord-293913-frkb8iso 1 15 J. J. NNP cord-293913-frkb8iso 1 16 ; ; : cord-293913-frkb8iso 1 17 Baker Baker NNP cord-293913-frkb8iso 1 18 , , , cord-293913-frkb8iso 1 19 Susan Susan NNP cord-293913-frkb8iso 1 20 C. C. NNP cord-293913-frkb8iso 1 21 title title NN cord-293913-frkb8iso 1 22 : : : cord-293913-frkb8iso 1 23 Identification identification NN cord-293913-frkb8iso 1 24 of of IN cord-293913-frkb8iso 1 25 the the DT cord-293913-frkb8iso 1 26 polymerase polymerase NN cord-293913-frkb8iso 1 27 polyprotein polyprotein NNP cord-293913-frkb8iso 1 28 products product NNS cord-293913-frkb8iso 1 29 p72 p72 NN cord-293913-frkb8iso 1 30 and and CC cord-293913-frkb8iso 1 31 p65 p65 NN cord-293913-frkb8iso 1 32 of of IN cord-293913-frkb8iso 1 33 the the DT cord-293913-frkb8iso 1 34 murine murine JJ cord-293913-frkb8iso 1 35 coronavirus coronavirus NN cord-293913-frkb8iso 1 36 MHV MHV NNP cord-293913-frkb8iso 1 37 - - HYPH cord-293913-frkb8iso 1 38 JHM JHM NNP cord-293913-frkb8iso 1 39 date date NN cord-293913-frkb8iso 1 40 : : : cord-293913-frkb8iso 1 41 1996 1996 CD cord-293913-frkb8iso 1 42 - - SYM cord-293913-frkb8iso 1 43 12 12 CD cord-293913-frkb8iso 1 44 - - SYM cord-293913-frkb8iso 1 45 31 31 CD cord-293913-frkb8iso 1 46 journal journal NN cord-293913-frkb8iso 1 47 : : : cord-293913-frkb8iso 2 1 Virus Virus NNP cord-293913-frkb8iso 2 2 Research Research NNP cord-293913-frkb8iso 2 3 DOI DOI NNP cord-293913-frkb8iso 2 4 : : : cord-293913-frkb8iso 3 1 10.1016 10.1016 CD cord-293913-frkb8iso 3 2 / / SYM cord-293913-frkb8iso 3 3 s0168 s0168 NNP cord-293913-frkb8iso 3 4 - - HYPH cord-293913-frkb8iso 3 5 1702(96)01368 1702(96)01368 NNP cord-293913-frkb8iso 3 6 - - HYPH cord-293913-frkb8iso 3 7 8 8 CD cord-293913-frkb8iso 3 8 sha sha NN cord-293913-frkb8iso 3 9 : : : cord-293913-frkb8iso 4 1 74afe9357aa764387a5a0ec524e83990232e838b 74afe9357aa764387a5a0ec524e83990232e838b NNP cord-293913-frkb8iso 5 1 doc_id doc_id CD cord-293913-frkb8iso 5 2 : : : cord-293913-frkb8iso 5 3 293913 293913 CD cord-293913-frkb8iso 5 4 cord_uid cord_uid NNS cord-293913-frkb8iso 5 5 : : : cord-293913-frkb8iso 6 1 frkb8iso frkb8iso NNP cord-293913-frkb8iso 7 1 Abstract Abstract NNP cord-293913-frkb8iso 8 1 The the DT cord-293913-frkb8iso 8 2 RNA RNA NNP cord-293913-frkb8iso 8 3 polymerase polymerase NN cord-293913-frkb8iso 8 4 gene gene NN cord-293913-frkb8iso 8 5 of of IN cord-293913-frkb8iso 8 6 murine murine JJ cord-293913-frkb8iso 8 7 coronavirus coronavirus NN cord-293913-frkb8iso 8 8 MHV MHV NNP cord-293913-frkb8iso 8 9 - - HYPH cord-293913-frkb8iso 8 10 JHM JHM NNP cord-293913-frkb8iso 8 11 encodes encode VBZ cord-293913-frkb8iso 8 12 a a DT cord-293913-frkb8iso 8 13 polyprotein polyprotein NN cord-293913-frkb8iso 8 14 of of IN cord-293913-frkb8iso 8 15 greater great JJR cord-293913-frkb8iso 8 16 than than IN cord-293913-frkb8iso 8 17 750 750 CD cord-293913-frkb8iso 8 18 kDa kDa NNS cord-293913-frkb8iso 8 19 . . . cord-293913-frkb8iso 9 1 This this DT cord-293913-frkb8iso 9 2 polyprotein polyprotein NN cord-293913-frkb8iso 9 3 is be VBZ cord-293913-frkb8iso 9 4 proposed propose VBN cord-293913-frkb8iso 9 5 to to TO cord-293913-frkb8iso 9 6 be be VB cord-293913-frkb8iso 9 7 processed process VBN cord-293913-frkb8iso 9 8 by by IN cord-293913-frkb8iso 9 9 two two CD cord-293913-frkb8iso 9 10 papain papain NN cord-293913-frkb8iso 9 11 - - HYPH cord-293913-frkb8iso 9 12 like like JJ cord-293913-frkb8iso 9 13 cysteine cysteine NN cord-293913-frkb8iso 9 14 proteinases proteinase NNS cord-293913-frkb8iso 9 15 , , , cord-293913-frkb8iso 9 16 PCP-1 PCP-1 NNP cord-293913-frkb8iso 9 17 and and CC cord-293913-frkb8iso 9 18 PCP-2 PCP-2 NNP cord-293913-frkb8iso 9 19 , , , cord-293913-frkb8iso 9 20 and and CC cord-293913-frkb8iso 9 21 a a DT cord-293913-frkb8iso 9 22 poliovirus poliovirus NN cord-293913-frkb8iso 9 23 3C 3c NN cord-293913-frkb8iso 9 24 - - HYPH cord-293913-frkb8iso 9 25 like like JJ cord-293913-frkb8iso 9 26 proteinase proteinase NN cord-293913-frkb8iso 9 27 domain domain NN cord-293913-frkb8iso 9 28 , , , cord-293913-frkb8iso 9 29 3C 3c NN cord-293913-frkb8iso 9 30 - - HYPH cord-293913-frkb8iso 9 31 pro pro NNP cord-293913-frkb8iso 9 32 , , , cord-293913-frkb8iso 9 33 to to TO cord-293913-frkb8iso 9 34 generate generate VB cord-293913-frkb8iso 9 35 protein protein NN cord-293913-frkb8iso 9 36 products product NNS cord-293913-frkb8iso 9 37 . . . cord-293913-frkb8iso 10 1 The the DT cord-293913-frkb8iso 10 2 amino amino NN cord-293913-frkb8iso 10 3 - - HYPH cord-293913-frkb8iso 10 4 terminal terminal NN cord-293913-frkb8iso 10 5 product product NN cord-293913-frkb8iso 10 6 of of IN cord-293913-frkb8iso 10 7 the the DT cord-293913-frkb8iso 10 8 MHV MHV NNP cord-293913-frkb8iso 10 9 polymerase polymerase NN cord-293913-frkb8iso 10 10 polyprotein polyprotein NN cord-293913-frkb8iso 10 11 , , , cord-293913-frkb8iso 10 12 p28 p28 CD cord-293913-frkb8iso 10 13 , , , cord-293913-frkb8iso 10 14 is be VBZ cord-293913-frkb8iso 10 15 generated generate VBN cord-293913-frkb8iso 10 16 by by IN cord-293913-frkb8iso 10 17 cleavage cleavage NN cord-293913-frkb8iso 10 18 of of IN cord-293913-frkb8iso 10 19 the the DT cord-293913-frkb8iso 10 20 polyprotein polyprotein NN cord-293913-frkb8iso 10 21 by by IN cord-293913-frkb8iso 10 22 PCP-1 PCP-1 NNP cord-293913-frkb8iso 10 23 . . . cord-293913-frkb8iso 11 1 To to TO cord-293913-frkb8iso 11 2 identify identify VB cord-293913-frkb8iso 11 3 the the DT cord-293913-frkb8iso 11 4 viral viral JJ cord-293913-frkb8iso 11 5 products product NNS cord-293913-frkb8iso 11 6 downstream downstream RB cord-293913-frkb8iso 11 7 of of IN cord-293913-frkb8iso 11 8 p28 p28 CD cord-293913-frkb8iso 11 9 , , , cord-293913-frkb8iso 11 10 we -PRON- PRP cord-293913-frkb8iso 11 11 generated generate VBD cord-293913-frkb8iso 11 12 a a DT cord-293913-frkb8iso 11 13 fusion fusion NN cord-293913-frkb8iso 11 14 - - HYPH cord-293913-frkb8iso 11 15 protein protein NN cord-293913-frkb8iso 11 16 specific specific JJ cord-293913-frkb8iso 11 17 antiserum antiserum NN cord-293913-frkb8iso 11 18 directed direct VBN cord-293913-frkb8iso 11 19 against against IN cord-293913-frkb8iso 11 20 the the DT cord-293913-frkb8iso 11 21 region region NN cord-293913-frkb8iso 11 22 adjacent adjacent JJ cord-293913-frkb8iso 11 23 to to IN cord-293913-frkb8iso 11 24 p28 p28 CD cord-293913-frkb8iso 11 25 and and CC cord-293913-frkb8iso 11 26 used use VBD cord-293913-frkb8iso 11 27 the the DT cord-293913-frkb8iso 11 28 antiserum antiserum NN cord-293913-frkb8iso 11 29 to to TO cord-293913-frkb8iso 11 30 detect detect VB cord-293913-frkb8iso 11 31 virus virus NN cord-293913-frkb8iso 11 32 - - HYPH cord-293913-frkb8iso 11 33 specific specific JJ cord-293913-frkb8iso 11 34 proteins protein NNS cord-293913-frkb8iso 11 35 from from IN cord-293913-frkb8iso 11 36 MHV MHV NNP cord-293913-frkb8iso 11 37 - - HYPH cord-293913-frkb8iso 11 38 JHM JHM NNP cord-293913-frkb8iso 11 39 infected infected JJ cord-293913-frkb8iso 11 40 cells cell NNS cord-293913-frkb8iso 11 41 . . . cord-293913-frkb8iso 12 1 When when WRB cord-293913-frkb8iso 12 2 this this DT cord-293913-frkb8iso 12 3 antiserum antiserum NN cord-293913-frkb8iso 12 4 was be VBD cord-293913-frkb8iso 12 5 used use VBN cord-293913-frkb8iso 12 6 to to TO cord-293913-frkb8iso 12 7 immunoprecipitate immunoprecipitate NNP cord-293913-frkb8iso 12 8 radiolabeled radiolabele VBD cord-293913-frkb8iso 12 9 proteins protein NNS cord-293913-frkb8iso 12 10 from from IN cord-293913-frkb8iso 12 11 MHV MHV NNP cord-293913-frkb8iso 12 12 - - HYPH cord-293913-frkb8iso 12 13 JHM JHM NNP cord-293913-frkb8iso 12 14 infected infected JJ cord-293913-frkb8iso 12 15 cell cell NN cord-293913-frkb8iso 12 16 lysates lysate NNS cord-293913-frkb8iso 12 17 , , , cord-293913-frkb8iso 12 18 virus virus NN cord-293913-frkb8iso 12 19 - - HYPH cord-293913-frkb8iso 12 20 specific specific JJ cord-293913-frkb8iso 12 21 proteins protein NNS cord-293913-frkb8iso 12 22 of of IN cord-293913-frkb8iso 12 23 72 72 CD cord-293913-frkb8iso 12 24 and and CC cord-293913-frkb8iso 12 25 65 65 CD cord-293913-frkb8iso 12 26 kDa kDa NNS cord-293913-frkb8iso 12 27 were be VBD cord-293913-frkb8iso 12 28 detected detect VBN cord-293913-frkb8iso 12 29 . . . cord-293913-frkb8iso 13 1 Furthermore furthermore RB cord-293913-frkb8iso 13 2 , , , cord-293913-frkb8iso 13 3 pulse pulse NN cord-293913-frkb8iso 13 4 and and CC cord-293913-frkb8iso 13 5 chase chase NN cord-293913-frkb8iso 13 6 experiments experiment NNS cord-293913-frkb8iso 13 7 demonstrated demonstrate VBD cord-293913-frkb8iso 13 8 that that IN cord-293913-frkb8iso 13 9 p72 p72 NN cord-293913-frkb8iso 13 10 is be VBZ cord-293913-frkb8iso 13 11 likely likely RB cord-293913-frkb8iso 13 12 a a DT cord-293913-frkb8iso 13 13 precursor precursor NN cord-293913-frkb8iso 13 14 to to IN cord-293913-frkb8iso 13 15 the the DT cord-293913-frkb8iso 13 16 mature mature JJ cord-293913-frkb8iso 13 17 protein protein NN cord-293913-frkb8iso 13 18 product product NN cord-293913-frkb8iso 13 19 , , , cord-293913-frkb8iso 13 20 p65 p65 ADD cord-293913-frkb8iso 13 21 . . . cord-293913-frkb8iso 14 1 To to TO cord-293913-frkb8iso 14 2 investigate investigate VB cord-293913-frkb8iso 14 3 which which WDT cord-293913-frkb8iso 14 4 viral viral JJ cord-293913-frkb8iso 14 5 proteinase proteinase NN cord-293913-frkb8iso 14 6 may may MD cord-293913-frkb8iso 14 7 be be VB cord-293913-frkb8iso 14 8 responsible responsible JJ cord-293913-frkb8iso 14 9 for for IN cord-293913-frkb8iso 14 10 generating generate VBG cord-293913-frkb8iso 14 11 p72 p72 NN cord-293913-frkb8iso 14 12 and and CC cord-293913-frkb8iso 14 13 p65 p65 ADD cord-293913-frkb8iso 14 14 , , , cord-293913-frkb8iso 14 15 we -PRON- PRP cord-293913-frkb8iso 14 16 expressed express VBD cord-293913-frkb8iso 14 17 the the DT cord-293913-frkb8iso 14 18 5′-region 5′-region CD cord-293913-frkb8iso 14 19 of of IN cord-293913-frkb8iso 14 20 the the DT cord-293913-frkb8iso 14 21 MHV MHV NNP cord-293913-frkb8iso 14 22 - - HYPH cord-293913-frkb8iso 14 23 JHM JHM NNP cord-293913-frkb8iso 14 24 RNA RNA NNP cord-293913-frkb8iso 14 25 polymerase polymerase NN cord-293913-frkb8iso 14 26 gene gene NN cord-293913-frkb8iso 14 27 including include VBG cord-293913-frkb8iso 14 28 the the DT cord-293913-frkb8iso 14 29 two two CD cord-293913-frkb8iso 14 30 papain papain NN cord-293913-frkb8iso 14 31 - - HYPH cord-293913-frkb8iso 14 32 like like JJ cord-293913-frkb8iso 14 33 cysteine cysteine NN cord-293913-frkb8iso 14 34 proteinase proteinase NN cord-293913-frkb8iso 14 35 domains domain NNS cord-293913-frkb8iso 14 36 in in IN cord-293913-frkb8iso 14 37 an an DT cord-293913-frkb8iso 14 38 in in FW cord-293913-frkb8iso 14 39 vitro vitro FW cord-293913-frkb8iso 14 40 transcription transcription NN cord-293913-frkb8iso 14 41 / / SYM cord-293913-frkb8iso 14 42 translation translation NN cord-293913-frkb8iso 14 43 system system NN cord-293913-frkb8iso 14 44 and and CC cord-293913-frkb8iso 14 45 analyzed analyze VBD cord-293913-frkb8iso 14 46 the the DT cord-293913-frkb8iso 14 47 translation translation NN cord-293913-frkb8iso 14 48 products product NNS cord-293913-frkb8iso 14 49 for for IN cord-293913-frkb8iso 14 50 proteolytic proteolytic JJ cord-293913-frkb8iso 14 51 processing processing NN cord-293913-frkb8iso 14 52 . . . cord-293913-frkb8iso 15 1 We -PRON- PRP cord-293913-frkb8iso 15 2 also also RB cord-293913-frkb8iso 15 3 cloned clone VBD cord-293913-frkb8iso 15 4 and and CC cord-293913-frkb8iso 15 5 expressed express VBD cord-293913-frkb8iso 15 6 the the DT cord-293913-frkb8iso 15 7 72 72 CD cord-293913-frkb8iso 15 8 kDa kDa NNP cord-293913-frkb8iso 15 9 region region NN cord-293913-frkb8iso 15 10 immediately immediately RB cord-293913-frkb8iso 15 11 downstream downstream RB cord-293913-frkb8iso 15 12 from from IN cord-293913-frkb8iso 15 13 p28 p28 CD cord-293913-frkb8iso 15 14 , , , cord-293913-frkb8iso 15 15 and and CC cord-293913-frkb8iso 15 16 tested test VBD cord-293913-frkb8iso 15 17 the the DT cord-293913-frkb8iso 15 18 ability ability NN cord-293913-frkb8iso 15 19 of of IN cord-293913-frkb8iso 15 20 in in FW cord-293913-frkb8iso 15 21 vitro vitro FW cord-293913-frkb8iso 15 22 translated translate VBN cord-293913-frkb8iso 15 23 PCP-1 PCP-1 NNP cord-293913-frkb8iso 15 24 and and CC cord-293913-frkb8iso 15 25 PCP-2 PCP-2 NNP cord-293913-frkb8iso 15 26 to to TO cord-293913-frkb8iso 15 27 cleave cleave VB cord-293913-frkb8iso 15 28 p72 p72 NN cord-293913-frkb8iso 15 29 to to IN cord-293913-frkb8iso 15 30 p65 p65 VB cord-293913-frkb8iso 15 31 in in IN cord-293913-frkb8iso 15 32 trans trans NNP cord-293913-frkb8iso 15 33 . . . cord-293913-frkb8iso 16 1 Our -PRON- PRP$ cord-293913-frkb8iso 16 2 results result NNS cord-293913-frkb8iso 16 3 indicate indicate VBP cord-293913-frkb8iso 16 4 that that IN cord-293913-frkb8iso 16 5 neither neither DT cord-293913-frkb8iso 16 6 viral viral JJ cord-293913-frkb8iso 16 7 proteinase proteinase NN cord-293913-frkb8iso 16 8 domain domain NN cord-293913-frkb8iso 16 9 PCP-1 PCP-1 NNP cord-293913-frkb8iso 16 10 nor nor CC cord-293913-frkb8iso 16 11 PCP-2 PCP-2 NNP cord-293913-frkb8iso 16 12 is be VBZ cord-293913-frkb8iso 16 13 capable capable JJ cord-293913-frkb8iso 16 14 of of IN cord-293913-frkb8iso 16 15 cleavage cleavage NN cord-293913-frkb8iso 16 16 of of IN cord-293913-frkb8iso 16 17 p72 p72 NN cord-293913-frkb8iso 16 18 to to TO cord-293913-frkb8iso 16 19 produce produce VB cord-293913-frkb8iso 16 20 p65 p65 NN cord-293913-frkb8iso 16 21 in in IN cord-293913-frkb8iso 16 22 vitro vitro FW cord-293913-frkb8iso 16 23 . . . cord-293913-frkb8iso 17 1 The the DT cord-293913-frkb8iso 17 2 role role NN cord-293913-frkb8iso 17 3 of of IN cord-293913-frkb8iso 17 4 MHV MHV NNP cord-293913-frkb8iso 17 5 proteinases proteinase VBZ cord-293913-frkb8iso 17 6 in in IN cord-293913-frkb8iso 17 7 the the DT cord-293913-frkb8iso 17 8 processing processing NN cord-293913-frkb8iso 17 9 of of IN cord-293913-frkb8iso 17 10 p72 p72 NN cord-293913-frkb8iso 17 11 and and CC cord-293913-frkb8iso 17 12 p65 p65 NN cord-293913-frkb8iso 17 13 is be VBZ cord-293913-frkb8iso 17 14 discussed discuss VBN cord-293913-frkb8iso 17 15 . . . cord-293913-frkb8iso 18 1 The the DT cord-293913-frkb8iso 18 2 MHV MHV NNP cord-293913-frkb8iso 18 3 genomic genomic JJ cord-293913-frkb8iso 18 4 RNA rna NN cord-293913-frkb8iso 18 5 is be VBZ cord-293913-frkb8iso 18 6 surrounded surround VBN cord-293913-frkb8iso 18 7 by by IN cord-293913-frkb8iso 18 8 a a DT cord-293913-frkb8iso 18 9 phosphoprotein phosphoprotein NN cord-293913-frkb8iso 18 10 to to TO cord-293913-frkb8iso 18 11 form form VB cord-293913-frkb8iso 18 12 a a DT cord-293913-frkb8iso 18 13 helical helical JJ cord-293913-frkb8iso 18 14 nucleocapsid nucleocapsid NN cord-293913-frkb8iso 18 15 structure structure NN cord-293913-frkb8iso 18 16 within within IN cord-293913-frkb8iso 18 17 a a DT cord-293913-frkb8iso 18 18 lipid lipid NN cord-293913-frkb8iso 18 19 envelope envelope NN cord-293913-frkb8iso 18 20 . . . cord-293913-frkb8iso 19 1 Upon upon IN cord-293913-frkb8iso 19 2 infection infection NN cord-293913-frkb8iso 19 3 , , , cord-293913-frkb8iso 19 4 the the DT cord-293913-frkb8iso 19 5 MHV MHV NNP cord-293913-frkb8iso 19 6 genomic genomic JJ cord-293913-frkb8iso 19 7 RNA RNA NNP cord-293913-frkb8iso 19 8 is be VBZ cord-293913-frkb8iso 19 9 translated translate VBN cord-293913-frkb8iso 19 10 to to TO cord-293913-frkb8iso 19 11 produce produce VB cord-293913-frkb8iso 19 12 a a DT cord-293913-frkb8iso 19 13 viral viral JJ cord-293913-frkb8iso 19 14 RNA rna NN cord-293913-frkb8iso 19 15 - - HYPH cord-293913-frkb8iso 19 16 dependent dependent JJ cord-293913-frkb8iso 19 17 RNA RNA NNP cord-293913-frkb8iso 19 18 polymerase polymerase NN cord-293913-frkb8iso 19 19 which which WDT cord-293913-frkb8iso 19 20 mediates mediate VBZ cord-293913-frkb8iso 19 21 the the DT cord-293913-frkb8iso 19 22 synthesis synthesis NN cord-293913-frkb8iso 19 23 of of IN cord-293913-frkb8iso 19 24 negative negative JJ cord-293913-frkb8iso 19 25 strand strand NN cord-293913-frkb8iso 19 26 RNA RNA NNP cord-293913-frkb8iso 19 27 and and CC cord-293913-frkb8iso 19 28 subgenomic subgenomic NNP cord-293913-frkb8iso 19 29 mRNAs mRNAs NNPS cord-293913-frkb8iso 19 30 . . . cord-293913-frkb8iso 20 1 The the DT cord-293913-frkb8iso 20 2 subgenomic subgenomic JJ cord-293913-frkb8iso 20 3 mRNAs mrnas WDT cord-293913-frkb8iso 20 4 form form VBP cord-293913-frkb8iso 20 5 a a DT cord-293913-frkb8iso 20 6 3'-coterminal 3'-coterminal CD cord-293913-frkb8iso 20 7 nested nest VBN cord-293913-frkb8iso 20 8 set set NN cord-293913-frkb8iso 20 9 and and CC cord-293913-frkb8iso 20 10 are be VBP cord-293913-frkb8iso 20 11 synthesized synthesize VBN cord-293913-frkb8iso 20 12 by by IN cord-293913-frkb8iso 20 13 a a DT cord-293913-frkb8iso 20 14 process process NN cord-293913-frkb8iso 20 15 that that WDT cord-293913-frkb8iso 20 16 involves involve VBZ cord-293913-frkb8iso 20 17 discontinuous discontinuous JJ cord-293913-frkb8iso 20 18 transcription transcription NN cord-293913-frkb8iso 21 1 ( ( -LRB- cord-293913-frkb8iso 21 2 Baric Baric NNP cord-293913-frkb8iso 21 3 et et NNP cord-293913-frkb8iso 21 4 al al NNP cord-293913-frkb8iso 21 5 . . . cord-293913-frkb8iso 22 1 , , , cord-293913-frkb8iso 22 2 1983 1983 CD cord-293913-frkb8iso 22 3 ; ; : cord-293913-frkb8iso 22 4 Spaan Spaan NNP cord-293913-frkb8iso 22 5 et et FW cord-293913-frkb8iso 22 6 al al NNP cord-293913-frkb8iso 22 7 . . NNP cord-293913-frkb8iso 22 8 , , , cord-293913-frkb8iso 22 9 1983 1983 CD cord-293913-frkb8iso 22 10 ) ) -RRB- cord-293913-frkb8iso 22 11 . . . cord-293913-frkb8iso 23 1 The the DT cord-293913-frkb8iso 23 2 discontinuous discontinuous JJ cord-293913-frkb8iso 23 3 transcription transcription NN cord-293913-frkb8iso 23 4 of of IN cord-293913-frkb8iso 23 5 MHV MHV NNP cord-293913-frkb8iso 23 6 RNA RNA NNP cord-293913-frkb8iso 23 7 is be VBZ cord-293913-frkb8iso 23 8 thought think VBN cord-293913-frkb8iso 23 9 to to TO cord-293913-frkb8iso 23 10 contribute contribute VB cord-293913-frkb8iso 23 11 to to IN cord-293913-frkb8iso 23 12 the the DT cord-293913-frkb8iso 23 13 high high JJ cord-293913-frkb8iso 23 14 frequency frequency NN cord-293913-frkb8iso 23 15 of of IN cord-293913-frkb8iso 23 16 RNA RNA NNP cord-293913-frkb8iso 23 17 recombination recombination NN cord-293913-frkb8iso 23 18 that that WDT cord-293913-frkb8iso 23 19 is be VBZ cord-293913-frkb8iso 23 20 also also RB cord-293913-frkb8iso 23 21 observed observe VBN cord-293913-frkb8iso 23 22 in in IN cord-293913-frkb8iso 23 23 MHV MHV NNP cord-293913-frkb8iso 23 24 - - HYPH cord-293913-frkb8iso 23 25 infected infect VBN cord-293913-frkb8iso 23 26 cells cell NNS cord-293913-frkb8iso 23 27 ( ( -LRB- cord-293913-frkb8iso 24 1 Makino Makino NNP cord-293913-frkb8iso 24 2 et et NNP cord-293913-frkb8iso 24 3 al al NNP cord-293913-frkb8iso 24 4 . . NNP cord-293913-frkb8iso 24 5 , , , cord-293913-frkb8iso 24 6 1986 1986 CD cord-293913-frkb8iso 24 7 ) ) -RRB- cord-293913-frkb8iso 24 8 . . . cord-293913-frkb8iso 25 1 The the DT cord-293913-frkb8iso 25 2 novel novel NN cord-293913-frkb8iso 25 3 transcription transcription NN cord-293913-frkb8iso 25 4 mechanism mechanism NN cord-293913-frkb8iso 25 5 proposed propose VBN cord-293913-frkb8iso 25 6 to to TO cord-293913-frkb8iso 25 7 generate generate VB cord-293913-frkb8iso 25 8 MHV MHV NNP cord-293913-frkb8iso 25 9 mRNAs mRNAs NNPS cord-293913-frkb8iso 25 10 and and CC cord-293913-frkb8iso 25 11 the the DT cord-293913-frkb8iso 25 12 high high JJ cord-293913-frkb8iso 25 13 frequency frequency NN cord-293913-frkb8iso 25 14 of of IN cord-293913-frkb8iso 25 15 MHV MHV NNP cord-293913-frkb8iso 25 16 RNA RNA NNP cord-293913-frkb8iso 25 17 recombination recombination NN cord-293913-frkb8iso 25 18 implicate implicate VBP cord-293913-frkb8iso 25 19 a a DT cord-293913-frkb8iso 25 20 polymerase polymerase NN cord-293913-frkb8iso 25 21 enzyme enzyme NN cord-293913-frkb8iso 25 22 with with IN cord-293913-frkb8iso 25 23 unusual unusual JJ cord-293913-frkb8iso 25 24 discontinuous discontinuous JJ cord-293913-frkb8iso 25 25 properties property NNS cord-293913-frkb8iso 25 26 ; ; : cord-293913-frkb8iso 25 27 however however RB cord-293913-frkb8iso 25 28 , , , cord-293913-frkb8iso 25 29 that that IN cord-293913-frkb8iso 25 30 polymerase polymerase NN cord-293913-frkb8iso 25 31 must must MD cord-293913-frkb8iso 25 32 also also RB cord-293913-frkb8iso 25 33 be be VB cord-293913-frkb8iso 25 34 capable capable JJ cord-293913-frkb8iso 25 35 of of IN cord-293913-frkb8iso 25 36 accurately accurately RB cord-293913-frkb8iso 25 37 replicating replicate VBG cord-293913-frkb8iso 25 38 the the DT cord-293913-frkb8iso 25 39 32 32 CD cord-293913-frkb8iso 25 40 kb kb NNP cord-293913-frkb8iso 25 41 genomic genomic JJ cord-293913-frkb8iso 25 42 RNA RNA NNP cord-293913-frkb8iso 25 43 to to TO cord-293913-frkb8iso 25 44 produce produce VB cord-293913-frkb8iso 25 45 infectious infectious JJ cord-293913-frkb8iso 25 46 virus virus NN cord-293913-frkb8iso 25 47 ( ( -LRB- cord-293913-frkb8iso 25 48 reviewed review VBN cord-293913-frkb8iso 25 49 by by IN cord-293913-frkb8iso 25 50 Lai Lai NNP cord-293913-frkb8iso 25 51 et et NNP cord-293913-frkb8iso 25 52 al al NNP cord-293913-frkb8iso 25 53 . . NNP cord-293913-frkb8iso 25 54 , , , cord-293913-frkb8iso 25 55 1994 1994 CD cord-293913-frkb8iso 25 56 ; ; . cord-293913-frkb8iso 26 1 van van NNP cord-293913-frkb8iso 26 2 der der NNP cord-293913-frkb8iso 26 3 Most Most NNP cord-293913-frkb8iso 26 4 and and CC cord-293913-frkb8iso 26 5 Spaan Spaan NNP cord-293913-frkb8iso 26 6 , , , cord-293913-frkb8iso 26 7 1995 1995 CD cord-293913-frkb8iso 26 8 ) ) -RRB- cord-293913-frkb8iso 26 9 . . . cord-293913-frkb8iso 27 1 To to TO cord-293913-frkb8iso 27 2 understand understand VB cord-293913-frkb8iso 27 3 the the DT cord-293913-frkb8iso 27 4 unique unique JJ cord-293913-frkb8iso 27 5 process process NN cord-293913-frkb8iso 27 6 of of IN cord-293913-frkb8iso 27 7 MHV MHV NNP cord-293913-frkb8iso 27 8 RNA RNA NNP cord-293913-frkb8iso 27 9 synthesis synthesis NN cord-293913-frkb8iso 27 10 , , , cord-293913-frkb8iso 27 11 we -PRON- PRP cord-293913-frkb8iso 27 12 aim aim VBP cord-293913-frkb8iso 27 13 to to TO cord-293913-frkb8iso 27 14 identify identify VB cord-293913-frkb8iso 27 15 the the DT cord-293913-frkb8iso 27 16 components component NNS cord-293913-frkb8iso 27 17 of of IN cord-293913-frkb8iso 27 18 the the DT cord-293913-frkb8iso 27 19 RNA RNA NNP cord-293913-frkb8iso 27 20 - - HYPH cord-293913-frkb8iso 27 21 dependent dependent JJ cord-293913-frkb8iso 27 22 RNA RNA NNP cord-293913-frkb8iso 27 23 polymerase polymerase NN cord-293913-frkb8iso 27 24 , , , cord-293913-frkb8iso 27 25 to to TO cord-293913-frkb8iso 27 26 determine determine VB cord-293913-frkb8iso 27 27 how how WRB cord-293913-frkb8iso 27 28 these these DT cord-293913-frkb8iso 27 29 proteins protein NNS cord-293913-frkb8iso 27 30 are be VBP cord-293913-frkb8iso 27 31 generated generate VBN cord-293913-frkb8iso 27 32 and and CC cord-293913-frkb8iso 27 33 to to TO cord-293913-frkb8iso 27 34 elucidate elucidate VB cord-293913-frkb8iso 27 35 their -PRON- PRP$ cord-293913-frkb8iso 27 36 function function NN cord-293913-frkb8iso 27 37 in in IN cord-293913-frkb8iso 27 38 the the DT cord-293913-frkb8iso 27 39 complex complex JJ cord-293913-frkb8iso 27 40 MHV MHV NNP cord-293913-frkb8iso 27 41 replication replication NN cord-293913-frkb8iso 27 42 strategy strategy NN cord-293913-frkb8iso 27 43 . . . cord-293913-frkb8iso 28 1 The the DT cord-293913-frkb8iso 28 2 MHV MHV NNP cord-293913-frkb8iso 28 3 RNA RNA NNP cord-293913-frkb8iso 28 4 - - HYPH cord-293913-frkb8iso 28 5 dependent dependent JJ cord-293913-frkb8iso 28 6 RNA RNA NNP cord-293913-frkb8iso 28 7 polymerase polymerase NN cord-293913-frkb8iso 28 8 is be VBZ cord-293913-frkb8iso 28 9 encoded encode VBN cord-293913-frkb8iso 28 10 by by IN cord-293913-frkb8iso 28 11 the the DT cord-293913-frkb8iso 28 12 5'-most 5'-most CD cord-293913-frkb8iso 28 13 gene gene NN cord-293913-frkb8iso 28 14 , , , cord-293913-frkb8iso 28 15 gene gene NN cord-293913-frkb8iso 28 16 1 1 CD cord-293913-frkb8iso 28 17 . . . cord-293913-frkb8iso 29 1 Gene gene NN cord-293913-frkb8iso 29 2 1 1 CD cord-293913-frkb8iso 29 3 is be VBZ cord-293913-frkb8iso 29 4 22 22 CD cord-293913-frkb8iso 29 5 kb kb NNS cord-293913-frkb8iso 29 6 and and CC cord-293913-frkb8iso 29 7 contains contain VBZ cord-293913-frkb8iso 29 8 two two CD cord-293913-frkb8iso 29 9 long long JJ cord-293913-frkb8iso 29 10 open open JJ cord-293913-frkb8iso 29 11 reading reading NN cord-293913-frkb8iso 29 12 frames frame NNS cord-293913-frkb8iso 29 13 ( ( -LRB- cord-293913-frkb8iso 29 14 ORFs ORFs NNP cord-293913-frkb8iso 29 15 ) ) -RRB- cord-293913-frkb8iso 29 16 designated designate VBN cord-293913-frkb8iso 29 17 ORF ORF NNP cord-293913-frkb8iso 29 18 la la NNP cord-293913-frkb8iso 29 19 and and CC cord-293913-frkb8iso 29 20 ORF ORF NNP cord-293913-frkb8iso 29 21 lb lb CD cord-293913-frkb8iso 30 1 ( ( -LRB- cord-293913-frkb8iso 30 2 Pachuk Pachuk NNP cord-293913-frkb8iso 30 3 et et NNP cord-293913-frkb8iso 30 4 al al NNP cord-293913-frkb8iso 30 5 . . . cord-293913-frkb8iso 31 1 , , , cord-293913-frkb8iso 31 2 1989 1989 CD cord-293913-frkb8iso 31 3 ; ; : cord-293913-frkb8iso 31 4 Lee Lee NNP cord-293913-frkb8iso 31 5 et et NNP cord-293913-frkb8iso 31 6 al al NNP cord-293913-frkb8iso 31 7 . . NNP cord-293913-frkb8iso 31 8 , , , cord-293913-frkb8iso 31 9 1991 1991 CD cord-293913-frkb8iso 31 10 ; ; : cord-293913-frkb8iso 31 11 Bonilla Bonilla NNP cord-293913-frkb8iso 31 12 et et NNP cord-293913-frkb8iso 31 13 al al NNP cord-293913-frkb8iso 31 14 . . NNP cord-293913-frkb8iso 31 15 , , , cord-293913-frkb8iso 31 16 1994 1994 CD cord-293913-frkb8iso 31 17 ) ) -RRB- cord-293913-frkb8iso 31 18 . . . cord-293913-frkb8iso 32 1 ORF ORF NNP cord-293913-frkb8iso 32 2 la la NNP cord-293913-frkb8iso 32 3 and and CC cord-293913-frkb8iso 32 4 ORF ORF NNP cord-293913-frkb8iso 32 5 lb lb CD cord-293913-frkb8iso 32 6 overlap overlap VBP cord-293913-frkb8iso 32 7 by by IN cord-293913-frkb8iso 32 8 75 75 CD cord-293913-frkb8iso 32 9 nucleotides nucleotide NNS cord-293913-frkb8iso 32 10 and and CC cord-293913-frkb8iso 32 11 may may MD cord-293913-frkb8iso 32 12 be be VB cord-293913-frkb8iso 32 13 translated translate VBN cord-293913-frkb8iso 32 14 to to TO cord-293913-frkb8iso 32 15 produce produce VB cord-293913-frkb8iso 32 16 a a DT cord-293913-frkb8iso 32 17 single single JJ cord-293913-frkb8iso 32 18 polyprotein polyprotein NN cord-293913-frkb8iso 32 19 of of IN cord-293913-frkb8iso 32 20 approximately approximately RB cord-293913-frkb8iso 32 21 750 750 CD cord-293913-frkb8iso 32 22 kDa kDa NNS cord-293913-frkb8iso 32 23 by by IN cord-293913-frkb8iso 32 24 a a DT cord-293913-frkb8iso 32 25 ribosomal ribosomal JJ cord-293913-frkb8iso 32 26 frameshift frameshift NN cord-293913-frkb8iso 32 27 mechanism mechanism NN cord-293913-frkb8iso 32 28 ( ( -LRB- cord-293913-frkb8iso 32 29 Brierly Brierly NNP cord-293913-frkb8iso 32 30 et et NNP cord-293913-frkb8iso 32 31 al al NNP cord-293913-frkb8iso 32 32 . . NNP cord-293913-frkb8iso 32 33 , , , cord-293913-frkb8iso 32 34 1987 1987 CD cord-293913-frkb8iso 33 1 ( ( -LRB- cord-293913-frkb8iso 33 2 Brierly Brierly NNP cord-293913-frkb8iso 33 3 et et NNP cord-293913-frkb8iso 33 4 al al NNP cord-293913-frkb8iso 33 5 . . NNP cord-293913-frkb8iso 33 6 , , , cord-293913-frkb8iso 33 7 , , , cord-293913-frkb8iso 33 8 1989 1989 CD cord-293913-frkb8iso 33 9 Bredenbeek Bredenbeek NNP cord-293913-frkb8iso 33 10 et et NNP cord-293913-frkb8iso 33 11 al al NNP cord-293913-frkb8iso 33 12 . . . cord-293913-frkb8iso 34 1 , , , cord-293913-frkb8iso 34 2 1990 1990 CD cord-293913-frkb8iso 34 3 ; ; : cord-293913-frkb8iso 34 4 Lee Lee NNP cord-293913-frkb8iso 34 5 et et NNP cord-293913-frkb8iso 34 6 al al NNP cord-293913-frkb8iso 34 7 . . NNP cord-293913-frkb8iso 34 8 , , , cord-293913-frkb8iso 34 9 1991 1991 CD cord-293913-frkb8iso 34 10 ) ) -RRB- cord-293913-frkb8iso 34 11 . . . cord-293913-frkb8iso 35 1 This this DT cord-293913-frkb8iso 35 2 large large JJ cord-293913-frkb8iso 35 3 polymerase polymerase NN cord-293913-frkb8iso 35 4 polyprotein polyprotein NN cord-293913-frkb8iso 35 5 is be VBZ cord-293913-frkb8iso 35 6 proposed propose VBN cord-293913-frkb8iso 35 7 to to TO cord-293913-frkb8iso 35 8 be be VB cord-293913-frkb8iso 35 9 processed process VBN cord-293913-frkb8iso 35 10 into into IN cord-293913-frkb8iso 35 11 mature mature JJ cord-293913-frkb8iso 35 12 protein protein NN cord-293913-frkb8iso 35 13 products product NNS cord-293913-frkb8iso 35 14 by by IN cord-293913-frkb8iso 35 15 viral viral JJ cord-293913-frkb8iso 35 16 proteinases proteinases NNP cord-293913-frkb8iso 35 17 encoded encode VBN cord-293913-frkb8iso 35 18 within within IN cord-293913-frkb8iso 35 19 the the DT cord-293913-frkb8iso 35 20 polyprotein polyprotein NN cord-293913-frkb8iso 35 21 , , , cord-293913-frkb8iso 35 22 and and CC cord-293913-frkb8iso 35 23 perhaps perhaps RB cord-293913-frkb8iso 35 24 by by IN cord-293913-frkb8iso 35 25 cellular cellular JJ cord-293913-frkb8iso 35 26 proteinases proteinase NNS cord-293913-frkb8iso 35 27 as as RB cord-293913-frkb8iso 35 28 well well RB cord-293913-frkb8iso 35 29 . . . cord-293913-frkb8iso 36 1 Three three CD cord-293913-frkb8iso 36 2 viral viral JJ cord-293913-frkb8iso 36 3 proteinase proteinase NN cord-293913-frkb8iso 36 4 domains domain NNS cord-293913-frkb8iso 36 5 , , , cord-293913-frkb8iso 36 6 including include VBG cord-293913-frkb8iso 36 7 two two CD cord-293913-frkb8iso 36 8 papainlike papainlike JJ cord-293913-frkb8iso 36 9 cysteine cysteine NN cord-293913-frkb8iso 36 10 proteinases proteinase NNS cord-293913-frkb8iso 36 11 ( ( -LRB- cord-293913-frkb8iso 36 12 PCP-1 PCP-1 NNP cord-293913-frkb8iso 36 13 and and CC cord-293913-frkb8iso 36 14 PCP-2 PCP-2 NNP cord-293913-frkb8iso 36 15 ) ) -RRB- cord-293913-frkb8iso 36 16 and and CC cord-293913-frkb8iso 36 17 a a DT cord-293913-frkb8iso 36 18 poliovirus poliovirus NN cord-293913-frkb8iso 36 19 3C 3c NN cord-293913-frkb8iso 36 20 - - HYPH cord-293913-frkb8iso 36 21 like like JJ cord-293913-frkb8iso 36 22 proteinase proteinase NN cord-293913-frkb8iso 36 23 domain domain NN cord-293913-frkb8iso 36 24 ( ( -LRB- cord-293913-frkb8iso 36 25 3C 3c NN cord-293913-frkb8iso 36 26 - - HYPH cord-293913-frkb8iso 36 27 pro pro NNP cord-293913-frkb8iso 36 28 ) ) -RRB- cord-293913-frkb8iso 36 29 , , , cord-293913-frkb8iso 36 30 have have VBP cord-293913-frkb8iso 36 31 been be VBN cord-293913-frkb8iso 36 32 predicted predict VBN cord-293913-frkb8iso 36 33 by by IN cord-293913-frkb8iso 36 34 amino amino NN cord-293913-frkb8iso 36 35 acid acid NN cord-293913-frkb8iso 36 36 sequence sequence NN cord-293913-frkb8iso 36 37 comparison comparison NN cord-293913-frkb8iso 36 38 ( ( -LRB- cord-293913-frkb8iso 36 39 Gorbalenya Gorbalenya NNP cord-293913-frkb8iso 36 40 et et NNP cord-293913-frkb8iso 36 41 al al NNP cord-293913-frkb8iso 36 42 . . NNP cord-293913-frkb8iso 36 43 , , , cord-293913-frkb8iso 36 44 1989 1989 CD cord-293913-frkb8iso 36 45 . . . cord-293913-frkb8iso 37 1 The the DT cord-293913-frkb8iso 37 2 first first JJ cord-293913-frkb8iso 37 3 direct direct JJ cord-293913-frkb8iso 37 4 evidence evidence NN cord-293913-frkb8iso 37 5 for for IN cord-293913-frkb8iso 37 6 proteolytic proteolytic JJ cord-293913-frkb8iso 37 7 processing processing NN cord-293913-frkb8iso 37 8 of of IN cord-293913-frkb8iso 37 9 the the DT cord-293913-frkb8iso 37 10 polymerase polymerase NN cord-293913-frkb8iso 37 11 polyprotein polyprotein NN cord-293913-frkb8iso 37 12 came come VBD cord-293913-frkb8iso 37 13 from from IN cord-293913-frkb8iso 37 14 in in IN cord-293913-frkb8iso 37 15 vitro vitro FW cord-293913-frkb8iso 37 16 translation translation NN cord-293913-frkb8iso 37 17 studies study NNS cord-293913-frkb8iso 37 18 of of IN cord-293913-frkb8iso 37 19 MHV MHV NNP cord-293913-frkb8iso 37 20 genomic genomic JJ cord-293913-frkb8iso 37 21 RNA rna NN cord-293913-frkb8iso 37 22 . . . cord-293913-frkb8iso 38 1 Denison Denison NNP cord-293913-frkb8iso 38 2 and and CC cord-293913-frkb8iso 38 3 Perlman Perlman NNP cord-293913-frkb8iso 38 4 showed show VBD cord-293913-frkb8iso 38 5 that that IN cord-293913-frkb8iso 38 6 in in IN cord-293913-frkb8iso 38 7 vitro vitro FW cord-293913-frkb8iso 38 8 translation translation NN cord-293913-frkb8iso 38 9 of of IN cord-293913-frkb8iso 38 10 viral viral JJ cord-293913-frkb8iso 38 11 genomic genomic JJ cord-293913-frkb8iso 38 12 RNA RNA NNP cord-293913-frkb8iso 38 13 produced produce VBD cord-293913-frkb8iso 38 14 p28 p28 CD cord-293913-frkb8iso 38 15 and and CC cord-293913-frkb8iso 38 16 p220 p220 NNP cord-293913-frkb8iso 38 17 , , , cord-293913-frkb8iso 38 18 and and CC cord-293913-frkb8iso 38 19 that that DT cord-293913-frkb8iso 38 20 production production NN cord-293913-frkb8iso 38 21 of of IN cord-293913-frkb8iso 38 22 p28 p28 NN cord-293913-frkb8iso 38 23 was be VBD cord-293913-frkb8iso 38 24 inhibited inhibit VBN cord-293913-frkb8iso 38 25 by by IN cord-293913-frkb8iso 38 26 the the DT cord-293913-frkb8iso 38 27 addition addition NN cord-293913-frkb8iso 38 28 of of IN cord-293913-frkb8iso 38 29 a a DT cord-293913-frkb8iso 38 30 proteinase proteinase NN cord-293913-frkb8iso 38 31 inhibitor inhibitor NN cord-293913-frkb8iso 38 32 , , , cord-293913-frkb8iso 38 33 zinc zinc NN cord-293913-frkb8iso 38 34 chloride chloride NN cord-293913-frkb8iso 38 35 ( ( -LRB- cord-293913-frkb8iso 38 36 Denison Denison NNP cord-293913-frkb8iso 38 37 and and CC cord-293913-frkb8iso 38 38 Perlman Perlman NNP cord-293913-frkb8iso 38 39 , , , cord-293913-frkb8iso 38 40 1986 1986 CD cord-293913-frkb8iso 38 41 ) ) -RRB- cord-293913-frkb8iso 38 42 . . . cord-293913-frkb8iso 39 1 Using use VBG cord-293913-frkb8iso 39 2 an an DT cord-293913-frkb8iso 39 3 antiserum antiserum NN cord-293913-frkb8iso 39 4 generated generate VBN cord-293913-frkb8iso 39 5 against against IN cord-293913-frkb8iso 39 6 a a DT cord-293913-frkb8iso 39 7 synthetic synthetic JJ cord-293913-frkb8iso 39 8 peptide peptide NN cord-293913-frkb8iso 39 9 representing represent VBG cord-293913-frkb8iso 39 10 a a DT cord-293913-frkb8iso 39 11 portion portion NN cord-293913-frkb8iso 39 12 of of IN cord-293913-frkb8iso 39 13 p28 p28 CD cord-293913-frkb8iso 39 14 , , , cord-293913-frkb8iso 39 15 the the DT cord-293913-frkb8iso 39 16 p28 p28 NNP cord-293913-frkb8iso 39 17 protein protein NN cord-293913-frkb8iso 39 18 was be VBD cord-293913-frkb8iso 39 19 detected detect VBN cord-293913-frkb8iso 39 20 in in IN cord-293913-frkb8iso 39 21 MHV mhv NN cord-293913-frkb8iso 39 22 - - HYPH cord-293913-frkb8iso 39 23 infected infect VBN cord-293913-frkb8iso 39 24 cells cell NNS cord-293913-frkb8iso 39 25 and and CC cord-293913-frkb8iso 39 26 shown show VBN cord-293913-frkb8iso 39 27 to to TO cord-293913-frkb8iso 39 28 be be VB cord-293913-frkb8iso 39 29 the the DT cord-293913-frkb8iso 39 30 amino amino NN cord-293913-frkb8iso 39 31 - - HYPH cord-293913-frkb8iso 39 32 terminal terminal NN cord-293913-frkb8iso 39 33 protein protein NN cord-293913-frkb8iso 39 34 of of IN cord-293913-frkb8iso 39 35 the the DT cord-293913-frkb8iso 39 36 polymerase polymerase NN cord-293913-frkb8iso 39 37 polyprotein polyprotein NN cord-293913-frkb8iso 39 38 ( ( -LRB- cord-293913-frkb8iso 39 39 Denison Denison NNP cord-293913-frkb8iso 39 40 and and CC cord-293913-frkb8iso 39 41 Perlman Perlman NNP cord-293913-frkb8iso 39 42 , , , cord-293913-frkb8iso 39 43 1987 1987 CD cord-293913-frkb8iso 39 44 ; ; : cord-293913-frkb8iso 40 1 Soe Soe NNP cord-293913-frkb8iso 40 2 et et FW cord-293913-frkb8iso 40 3 al al NNP cord-293913-frkb8iso 40 4 . . NNP cord-293913-frkb8iso 40 5 , , , cord-293913-frkb8iso 40 6 1987 1987 CD cord-293913-frkb8iso 40 7 ) ) -RRB- cord-293913-frkb8iso 40 8 . . . cord-293913-frkb8iso 41 1 Translation translation NN cord-293913-frkb8iso 41 2 of of IN cord-293913-frkb8iso 41 3 RNAs rna NNS cord-293913-frkb8iso 41 4 representing represent VBG cord-293913-frkb8iso 41 5 the the DT cord-293913-frkb8iso 41 6 5 5 CD cord-293913-frkb8iso 41 7 ' ' NN cord-293913-frkb8iso 41 8 end end NN cord-293913-frkb8iso 41 9 of of IN cord-293913-frkb8iso 41 10 the the DT cord-293913-frkb8iso 41 11 viral viral JJ cord-293913-frkb8iso 41 12 genome genome NN cord-293913-frkb8iso 41 13 revealed reveal VBD cord-293913-frkb8iso 41 14 that that IN cord-293913-frkb8iso 41 15 the the DT cord-293913-frkb8iso 41 16 PCP-1 PCP-1 NNP cord-293913-frkb8iso 41 17 domain domain NN cord-293913-frkb8iso 41 18 was be VBD cord-293913-frkb8iso 41 19 required require VBN cord-293913-frkb8iso 41 20 for for IN cord-293913-frkb8iso 41 21 the the DT cord-293913-frkb8iso 41 22 autoproteolytic autoproteolytic JJ cord-293913-frkb8iso 41 23 cleavage cleavage NN cord-293913-frkb8iso 41 24 of of IN cord-293913-frkb8iso 41 25 p28 p28 NNP cord-293913-frkb8iso 41 26 ( ( -LRB- cord-293913-frkb8iso 41 27 Baker Baker NNP cord-293913-frkb8iso 41 28 et et NNP cord-293913-frkb8iso 41 29 al al NNP cord-293913-frkb8iso 41 30 . . NNP cord-293913-frkb8iso 41 31 , , , cord-293913-frkb8iso 41 32 1989 1989 CD cord-293913-frkb8iso 41 33 ) ) -RRB- cord-293913-frkb8iso 41 34 . . . cord-293913-frkb8iso 42 1 The the DT cord-293913-frkb8iso 42 2 cleavage cleavage NN cord-293913-frkb8iso 42 3 of of IN cord-293913-frkb8iso 42 4 p28 p28 NN cord-293913-frkb8iso 42 5 by by IN cord-293913-frkb8iso 42 6 PCP-1 PCP-1 NNP cord-293913-frkb8iso 42 7 was be VBD cord-293913-frkb8iso 42 8 inhibited inhibit VBN cord-293913-frkb8iso 42 9 by by IN cord-293913-frkb8iso 42 10 leupeptin leupeptin NN cord-293913-frkb8iso 42 11 in in IN cord-293913-frkb8iso 42 12 both both DT cord-293913-frkb8iso 42 13 in in IN cord-293913-frkb8iso 42 14 vivo vivo NN cord-293913-frkb8iso 42 15 and and CC cord-293913-frkb8iso 42 16 in in IN cord-293913-frkb8iso 42 17 vitro vitro FW cord-293913-frkb8iso 42 18 reactions reaction NNS cord-293913-frkb8iso 42 19 ( ( -LRB- cord-293913-frkb8iso 42 20 Denison Denison NNP cord-293913-frkb8iso 42 21 and and CC cord-293913-frkb8iso 42 22 Perlman Perlman NNP cord-293913-frkb8iso 42 23 , , , cord-293913-frkb8iso 42 24 1986 1986 CD cord-293913-frkb8iso 42 25 ; ; : cord-293913-frkb8iso 42 26 Baker Baker NNP cord-293913-frkb8iso 42 27 et et NNP cord-293913-frkb8iso 42 28 al al NNP cord-293913-frkb8iso 42 29 . . NNP cord-293913-frkb8iso 42 30 , , , cord-293913-frkb8iso 42 31 1989 1989 CD cord-293913-frkb8iso 42 32 ) ) -RRB- cord-293913-frkb8iso 42 33 . . . cord-293913-frkb8iso 43 1 Furthermore furthermore RB cord-293913-frkb8iso 43 2 , , , cord-293913-frkb8iso 43 3 the the DT cord-293913-frkb8iso 43 4 catalytic catalytic JJ cord-293913-frkb8iso 43 5 cysteine cysteine NN cord-293913-frkb8iso 43 6 and and CC cord-293913-frkb8iso 43 7 histidine histidine NN cord-293913-frkb8iso 43 8 residues residue NNS cord-293913-frkb8iso 43 9 of of IN cord-293913-frkb8iso 43 10 the the DT cord-293913-frkb8iso 43 11 PCP-1 PCP-1 NNP cord-293913-frkb8iso 43 12 domain domain NN cord-293913-frkb8iso 43 13 have have VBP cord-293913-frkb8iso 43 14 been be VBN cord-293913-frkb8iso 43 15 identified identify VBN cord-293913-frkb8iso 43 16 ( ( -LRB- cord-293913-frkb8iso 43 17 Baker Baker NNP cord-293913-frkb8iso 43 18 et et NNP cord-293913-frkb8iso 43 19 al al NNP cord-293913-frkb8iso 43 20 . . NNP cord-293913-frkb8iso 43 21 , , , cord-293913-frkb8iso 43 22 1993 1993 CD cord-293913-frkb8iso 43 23 ) ) -RRB- cord-293913-frkb8iso 43 24 . . . cord-293913-frkb8iso 44 1 The the DT cord-293913-frkb8iso 44 2 cleavage cleavage NN cord-293913-frkb8iso 44 3 site site NN cord-293913-frkb8iso 44 4 recognized recognize VBN cord-293913-frkb8iso 44 5 by by IN cord-293913-frkb8iso 44 6 PCP-1 PCP-1 NNP cord-293913-frkb8iso 44 7 for for IN cord-293913-frkb8iso 44 8 the the DT cord-293913-frkb8iso 44 9 cleavage cleavage NN cord-293913-frkb8iso 44 10 of of IN cord-293913-frkb8iso 44 11 p28 p28 NN cord-293913-frkb8iso 44 12 has have VBZ cord-293913-frkb8iso 44 13 been be VBN cord-293913-frkb8iso 44 14 determined determine VBN cord-293913-frkb8iso 44 15 to to TO cord-293913-frkb8iso 44 16 be be VB cord-293913-frkb8iso 44 17 the the DT cord-293913-frkb8iso 44 18 Gly-247 Gly-247 NNP cord-293913-frkb8iso 44 19 / / SYM cord-293913-frkb8iso 44 20 Val-248 Val-248 NNP cord-293913-frkb8iso 44 21 dipeptide dipeptide NN cord-293913-frkb8iso 44 22 bond bond NN cord-293913-frkb8iso 44 23 ( ( -LRB- cord-293913-frkb8iso 44 24 Dong Dong NNP cord-293913-frkb8iso 44 25 and and CC cord-293913-frkb8iso 44 26 Baker Baker NNP cord-293913-frkb8iso 44 27 , , , cord-293913-frkb8iso 44 28 1994 1994 CD cord-293913-frkb8iso 44 29 ; ; : cord-293913-frkb8iso 44 30 Hughes Hughes NNP cord-293913-frkb8iso 44 31 et et NNP cord-293913-frkb8iso 44 32 al al NNP cord-293913-frkb8iso 44 33 . . NNP cord-293913-frkb8iso 44 34 , , , cord-293913-frkb8iso 44 35 1995 1995 CD cord-293913-frkb8iso 44 36 ) ) -RRB- cord-293913-frkb8iso 44 37 . . . cord-293913-frkb8iso 45 1 A a DT cord-293913-frkb8iso 45 2 number number NN cord-293913-frkb8iso 45 3 of of IN cord-293913-frkb8iso 45 4 potential potential JJ cord-293913-frkb8iso 45 5 PCP-1 PCP-1 NNP cord-293913-frkb8iso 45 6 cleavage cleavage NN cord-293913-frkb8iso 45 7 sites site NNS cord-293913-frkb8iso 45 8 are be VBP cord-293913-frkb8iso 45 9 predicted predict VBN cord-293913-frkb8iso 45 10 , , , cord-293913-frkb8iso 45 11 but but CC cord-293913-frkb8iso 45 12 only only RB cord-293913-frkb8iso 45 13 the the DT cord-293913-frkb8iso 45 14 p28 p28 CD cord-293913-frkb8iso 45 15 cleavage cleavage NN cord-293913-frkb8iso 45 16 site site NN cord-293913-frkb8iso 45 17 has have VBZ cord-293913-frkb8iso 45 18 been be VBN cord-293913-frkb8iso 45 19 experimentally experimentally RB cord-293913-frkb8iso 45 20 confirmed confirm VBN cord-293913-frkb8iso 45 21 . . . cord-293913-frkb8iso 46 1 A a DT cord-293913-frkb8iso 46 2 second second JJ cord-293913-frkb8iso 46 3 , , , cord-293913-frkb8iso 46 4 independent independent JJ cord-293913-frkb8iso 46 5 cleavage cleavage NN cord-293913-frkb8iso 46 6 event event NN cord-293913-frkb8iso 46 7 has have VBZ cord-293913-frkb8iso 46 8 recently recently RB cord-293913-frkb8iso 46 9 been be VBN cord-293913-frkb8iso 46 10 implicated implicate VBN cord-293913-frkb8iso 46 11 in in IN cord-293913-frkb8iso 46 12 the the DT cord-293913-frkb8iso 46 13 release release NN cord-293913-frkb8iso 46 14 of of IN cord-293913-frkb8iso 46 15 a a DT cord-293913-frkb8iso 46 16 65 65 CD cord-293913-frkb8iso 46 17 kDa kDa NNS cord-293913-frkb8iso 46 18 product product NN cord-293913-frkb8iso 46 19 in in IN cord-293913-frkb8iso 46 20 MHV MHV NNP cord-293913-frkb8iso 46 21 - - HYPH cord-293913-frkb8iso 46 22 A59 A59 NNP cord-293913-frkb8iso 46 23 infected infected JJ cord-293913-frkb8iso 46 24 cells cell NNS cord-293913-frkb8iso 46 25 . . . cord-293913-frkb8iso 47 1 Using use VBG cord-293913-frkb8iso 47 2 an an DT cord-293913-frkb8iso 47 3 antiserum antiserum NN cord-293913-frkb8iso 47 4 which which WDT cord-293913-frkb8iso 47 5 was be VBD cord-293913-frkb8iso 47 6 directed direct VBN cord-293913-frkb8iso 47 7 against against IN cord-293913-frkb8iso 47 8 a a DT cord-293913-frkb8iso 47 9 fusion fusion NN cord-293913-frkb8iso 47 10 protein protein NN cord-293913-frkb8iso 47 11 encoding encode VBG cord-293913-frkb8iso 47 12 the the DT cord-293913-frkb8iso 47 13 entire entire JJ cord-293913-frkb8iso 47 14 p28 p28 CD cord-293913-frkb8iso 47 15 domain domain NN cord-293913-frkb8iso 47 16 and and CC cord-293913-frkb8iso 47 17 the the DT cord-293913-frkb8iso 47 18 region region NN cord-293913-frkb8iso 47 19 adjacent adjacent JJ cord-293913-frkb8iso 47 20 to to IN cord-293913-frkb8iso 47 21 the the DT cord-293913-frkb8iso 47 22 p28 p28 CD cord-293913-frkb8iso 47 23 cleavage cleavage NN cord-293913-frkb8iso 47 24 site site NN cord-293913-frkb8iso 47 25 , , , cord-293913-frkb8iso 47 26 Denison Denison NNP cord-293913-frkb8iso 47 27 and and CC cord-293913-frkb8iso 47 28 co co NNS cord-293913-frkb8iso 47 29 - - NNS cord-293913-frkb8iso 47 30 workers worker NNS cord-293913-frkb8iso 48 1 immunoprecipitated immunoprecipitate VBD cord-293913-frkb8iso 48 2 a a DT cord-293913-frkb8iso 48 3 65 65 CD cord-293913-frkb8iso 48 4 kDa kda NN cord-293913-frkb8iso 48 5 protein protein NN cord-293913-frkb8iso 48 6 from from IN cord-293913-frkb8iso 48 7 MHV MHV NNP cord-293913-frkb8iso 48 8 - - HYPH cord-293913-frkb8iso 48 9 A59 A59 NNP cord-293913-frkb8iso 48 10 infected infected JJ cord-293913-frkb8iso 48 11 cells cell NNS cord-293913-frkb8iso 48 12 . . . cord-293913-frkb8iso 49 1 Additional additional JJ cord-293913-frkb8iso 49 2 in in IN cord-293913-frkb8iso 49 3 vitro vitro FW cord-293913-frkb8iso 49 4 experiments experiment NNS cord-293913-frkb8iso 49 5 using use VBG cord-293913-frkb8iso 49 6 cloned clone VBN cord-293913-frkb8iso 49 7 cDNAs cdna NNS cord-293913-frkb8iso 49 8 of of IN cord-293913-frkb8iso 49 9 MHV MHV NNP cord-293913-frkb8iso 49 10 - - HYPH cord-293913-frkb8iso 49 11 A59 A59 NNP cord-293913-frkb8iso 49 12 suggest suggest VBP cord-293913-frkb8iso 49 13 that that IN cord-293913-frkb8iso 49 14 p65 p65 PRP cord-293913-frkb8iso 49 15 may may MD cord-293913-frkb8iso 49 16 be be VB cord-293913-frkb8iso 49 17 cleaved cleave VBN cord-293913-frkb8iso 49 18 by by IN cord-293913-frkb8iso 49 19 the the DT cord-293913-frkb8iso 49 20 5'-most 5'-most CD cord-293913-frkb8iso 49 21 proteinase proteinase NN cord-293913-frkb8iso 49 22 PCP-1 PCP-1 NNP cord-293913-frkb8iso 49 23 . . . cord-293913-frkb8iso 50 1 However however RB cord-293913-frkb8iso 50 2 , , , cord-293913-frkb8iso 50 3 Kim Kim NNP cord-293913-frkb8iso 50 4 et et NNP cord-293913-frkb8iso 50 5 al al NNP cord-293913-frkb8iso 50 6 . . . cord-293913-frkb8iso 51 1 ( ( -LRB- cord-293913-frkb8iso 51 2 1995 1995 CD cord-293913-frkb8iso 51 3 ) ) -RRB- cord-293913-frkb8iso 51 4 have have VBP cord-293913-frkb8iso 51 5 shown show VBN cord-293913-frkb8iso 51 6 that that IN cord-293913-frkb8iso 51 7 E64d e64d NN cord-293913-frkb8iso 51 8 , , , cord-293913-frkb8iso 51 9 an an DT cord-293913-frkb8iso 51 10 irreversible irreversible JJ cord-293913-frkb8iso 51 11 inhibitor inhibitor NN cord-293913-frkb8iso 51 12 of of IN cord-293913-frkb8iso 51 13 cysteine cysteine NN cord-293913-frkb8iso 51 14 proteinases proteinase NNS cord-293913-frkb8iso 51 15 , , , cord-293913-frkb8iso 51 16 specifically specifically RB cord-293913-frkb8iso 51 17 blocks block VBZ cord-293913-frkb8iso 51 18 the the DT cord-293913-frkb8iso 51 19 in in IN cord-293913-frkb8iso 51 20 vivo vivo NN cord-293913-frkb8iso 51 21 cleavage cleavage NN cord-293913-frkb8iso 51 22 of of IN cord-293913-frkb8iso 51 23 p65 p65 NN cord-293913-frkb8iso 51 24 but but CC cord-293913-frkb8iso 51 25 surprisingly surprisingly RB cord-293913-frkb8iso 51 26 has have VBZ cord-293913-frkb8iso 51 27 very very RB cord-293913-frkb8iso 51 28 little little JJ cord-293913-frkb8iso 51 29 effect effect NN cord-293913-frkb8iso 51 30 on on IN cord-293913-frkb8iso 51 31 the the DT cord-293913-frkb8iso 51 32 cleavage cleavage NN cord-293913-frkb8iso 51 33 of of IN cord-293913-frkb8iso 51 34 p28 p28 CD cord-293913-frkb8iso 51 35 . . . cord-293913-frkb8iso 52 1 This this DT cord-293913-frkb8iso 52 2 suggests suggest VBZ cord-293913-frkb8iso 52 3 that that IN cord-293913-frkb8iso 52 4 a a DT cord-293913-frkb8iso 52 5 proteinase proteinase NN cord-293913-frkb8iso 52 6 other other JJ cord-293913-frkb8iso 52 7 than than IN cord-293913-frkb8iso 52 8 PCP-1 PCP-1 NNP cord-293913-frkb8iso 52 9 , , , cord-293913-frkb8iso 52 10 which which WDT cord-293913-frkb8iso 52 11 cleaves cleave VBZ cord-293913-frkb8iso 52 12 p28 p28 CD cord-293913-frkb8iso 52 13 , , , cord-293913-frkb8iso 52 14 is be VBZ cord-293913-frkb8iso 52 15 responsible responsible JJ cord-293913-frkb8iso 52 16 for for IN cord-293913-frkb8iso 52 17 the the DT cord-293913-frkb8iso 52 18 liberation liberation NN cord-293913-frkb8iso 52 19 of of IN cord-293913-frkb8iso 52 20 p65 p65 ADD cord-293913-frkb8iso 52 21 . . . cord-293913-frkb8iso 53 1 In in IN cord-293913-frkb8iso 53 2 this this DT cord-293913-frkb8iso 53 3 study study NN cord-293913-frkb8iso 53 4 , , , cord-293913-frkb8iso 53 5 we -PRON- PRP cord-293913-frkb8iso 53 6 aimed aim VBD cord-293913-frkb8iso 53 7 to to TO cord-293913-frkb8iso 53 8 identify identify VB cord-293913-frkb8iso 53 9 the the DT cord-293913-frkb8iso 53 10 proteolytic proteolytic JJ cord-293913-frkb8iso 53 11 product product NN cord-293913-frkb8iso 53 12 ad ad NN cord-293913-frkb8iso 53 13 - - HYPH cord-293913-frkb8iso 53 14 jacent jacent NN cord-293913-frkb8iso 53 15 to to IN cord-293913-frkb8iso 53 16 p28 p28 CD cord-293913-frkb8iso 53 17 in in IN cord-293913-frkb8iso 53 18 the the DT cord-293913-frkb8iso 53 19 MHV MHV NNP cord-293913-frkb8iso 53 20 - - HYPH cord-293913-frkb8iso 53 21 JHM JHM NNP cord-293913-frkb8iso 53 22 polymerase polymerase NN cord-293913-frkb8iso 53 23 polyprotein polyprotein NN cord-293913-frkb8iso 53 24 and and CC cord-293913-frkb8iso 53 25 to to TO cord-293913-frkb8iso 53 26 determine determine VB cord-293913-frkb8iso 53 27 how how WRB cord-293913-frkb8iso 53 28 that that DT cord-293913-frkb8iso 53 29 protein protein NN cord-293913-frkb8iso 53 30 is be VBZ cord-293913-frkb8iso 53 31 processed process VBN cord-293913-frkb8iso 53 32 . . . cord-293913-frkb8iso 54 1 To to TO cord-293913-frkb8iso 54 2 examine examine VB cord-293913-frkb8iso 54 3 the the DT cord-293913-frkb8iso 54 4 synthesis synthesis NN cord-293913-frkb8iso 54 5 and and CC cord-293913-frkb8iso 54 6 processing processing NN cord-293913-frkb8iso 54 7 of of IN cord-293913-frkb8iso 54 8 the the DT cord-293913-frkb8iso 54 9 MHV MHV NNP cord-293913-frkb8iso 54 10 - - HYPH cord-293913-frkb8iso 54 11 JHM JHM NNP cord-293913-frkb8iso 54 12 polymerase polymerase NN cord-293913-frkb8iso 54 13 polyprotein polyprotein NNP cord-293913-frkb8iso 54 14 , , , cord-293913-frkb8iso 54 15 we -PRON- PRP cord-293913-frkb8iso 54 16 used use VBD cord-293913-frkb8iso 54 17 antisera antisera NNP cord-293913-frkb8iso 54 18 directed direct VBN cord-293913-frkb8iso 54 19 against against IN cord-293913-frkb8iso 54 20 the the DT cord-293913-frkb8iso 54 21 amino amino NN cord-293913-frkb8iso 54 22 - - HYPH cord-293913-frkb8iso 54 23 terminal terminal NN cord-293913-frkb8iso 54 24 domains domain NNS cord-293913-frkb8iso 54 25 of of IN cord-293913-frkb8iso 54 26 the the DT cord-293913-frkb8iso 54 27 polymerase polymerase NN cord-293913-frkb8iso 54 28 , , , cord-293913-frkb8iso 54 29 anti anti JJ cord-293913-frkb8iso 54 30 - - JJ cord-293913-frkb8iso 54 31 p28 p28 JJ cord-293913-frkb8iso 54 32 and and CC cord-293913-frkb8iso 54 33 anti-647 anti-647 NNP cord-293913-frkb8iso 54 34 , , , cord-293913-frkb8iso 54 35 to to TO cord-293913-frkb8iso 54 36 immunoprecipitate immunoprecipitate VB cord-293913-frkb8iso 54 37 [ [ -LRB- cord-293913-frkb8iso 54 38 35S]methionine 35s]methionine CD cord-293913-frkb8iso 54 39 - - HYPH cord-293913-frkb8iso 54 40 labeled label VBN cord-293913-frkb8iso 54 41 proteins protein NNS cord-293913-frkb8iso 54 42 from from IN cord-293913-frkb8iso 54 43 MHV MHV NNP cord-293913-frkb8iso 54 44 - - HYPH cord-293913-frkb8iso 54 45 JHM JHM NNP cord-293913-frkb8iso 54 46 infected infected JJ cord-293913-frkb8iso 54 47 cells cell NNS cord-293913-frkb8iso 54 48 . . . cord-293913-frkb8iso 55 1 The the DT cord-293913-frkb8iso 55 2 anti anti JJ cord-293913-frkb8iso 55 3 - - JJ cord-293913-frkb8iso 55 4 p28 p28 JJ cord-293913-frkb8iso 55 5 serum serum NN cord-293913-frkb8iso 55 6 was be VBD cord-293913-frkb8iso 55 7 generated generate VBN cord-293913-frkb8iso 55 8 using use VBG cord-293913-frkb8iso 55 9 a a DT cord-293913-frkb8iso 55 10 synthetic synthetic JJ cord-293913-frkb8iso 55 11 peptide peptide NN cord-293913-frkb8iso 55 12 representing represent VBG cord-293913-frkb8iso 55 13 amino amino JJ cord-293913-frkb8iso 55 14 acids acid NNS cord-293913-frkb8iso 55 15 78 78 CD cord-293913-frkb8iso 55 16 - - SYM cord-293913-frkb8iso 55 17 93 93 CD cord-293913-frkb8iso 55 18 of of IN cord-293913-frkb8iso 55 19 MHV MHV NNP cord-293913-frkb8iso 55 20 - - HYPH cord-293913-frkb8iso 55 21 JHM JHM NNP cord-293913-frkb8iso 55 22 and and CC cord-293913-frkb8iso 55 23 has have VBZ cord-293913-frkb8iso 55 24 been be VBN cord-293913-frkb8iso 55 25 previously previously RB cord-293913-frkb8iso 55 26 described describe VBN cord-293913-frkb8iso 55 27 ( ( -LRB- cord-293913-frkb8iso 55 28 Baker Baker NNP cord-293913-frkb8iso 55 29 et et NNP cord-293913-frkb8iso 55 30 al al NNP cord-293913-frkb8iso 55 31 . . NNP cord-293913-frkb8iso 55 32 , , , cord-293913-frkb8iso 55 33 1989 1989 CD cord-293913-frkb8iso 55 34 ) ) -RRB- cord-293913-frkb8iso 55 35 . . . cord-293913-frkb8iso 56 1 For for IN cord-293913-frkb8iso 56 2 this this DT cord-293913-frkb8iso 56 3 study study NN cord-293913-frkb8iso 56 4 , , , cord-293913-frkb8iso 56 5 the the DT cord-293913-frkb8iso 56 6 anti-647 anti-647 NNP cord-293913-frkb8iso 56 7 serum serum NN cord-293913-frkb8iso 56 8 was be VBD cord-293913-frkb8iso 56 9 generated generate VBN cord-293913-frkb8iso 56 10 against against IN cord-293913-frkb8iso 56 11 a a DT cord-293913-frkb8iso 56 12 bacterial bacterial JJ cord-293913-frkb8iso 56 13 GST GST NNP cord-293913-frkb8iso 56 14 - - HYPH cord-293913-frkb8iso 56 15 MHV mhv NN cord-293913-frkb8iso 56 16 fusion fusion NN cord-293913-frkb8iso 56 17 protein protein NN cord-293913-frkb8iso 56 18 which which WDT cord-293913-frkb8iso 56 19 encodes encode VBZ cord-293913-frkb8iso 56 20 a a DT cord-293913-frkb8iso 56 21 647 647 CD cord-293913-frkb8iso 56 22 basepair basepair NN cord-293913-frkb8iso 56 23 domain domain NN cord-293913-frkb8iso 56 24 that that WDT cord-293913-frkb8iso 56 25 is be VBZ cord-293913-frkb8iso 56 26 adjacent adjacent JJ cord-293913-frkb8iso 56 27 to to IN cord-293913-frkb8iso 56 28 p28 p28 CD cord-293913-frkb8iso 56 29 ( ( -LRB- cord-293913-frkb8iso 56 30 see see VB cord-293913-frkb8iso 56 31 Fig Fig NNP cord-293913-frkb8iso 56 32 . . NNP cord-293913-frkb8iso 56 33 1 1 CD cord-293913-frkb8iso 56 34 ) ) -RRB- cord-293913-frkb8iso 56 35 . . . cord-293913-frkb8iso 57 1 The the DT cord-293913-frkb8iso 57 2 bacterial bacterial JJ cord-293913-frkb8iso 57 3 expression expression NN cord-293913-frkb8iso 57 4 vector vector NN cord-293913-frkb8iso 57 5 , , , cord-293913-frkb8iso 57 6 pGEX pgex JJ cord-293913-frkb8iso 57 7 - - HYPH cord-293913-frkb8iso 57 8 KG KG NNP cord-293913-frkb8iso 57 9 , , , cord-293913-frkb8iso 57 10 which which WDT cord-293913-frkb8iso 57 11 contains contain VBZ cord-293913-frkb8iso 57 12 the the DT cord-293913-frkb8iso 57 13 glutathione glutathione NN cord-293913-frkb8iso 57 14 S s NN cord-293913-frkb8iso 57 15 - - HYPH cord-293913-frkb8iso 57 16 transferase transferase NN cord-293913-frkb8iso 57 17 ( ( -LRB- cord-293913-frkb8iso 57 18 GST gst NN cord-293913-frkb8iso 57 19 ) ) -RRB- cord-293913-frkb8iso 57 20 gene gene NN cord-293913-frkb8iso 57 21 under under IN cord-293913-frkb8iso 57 22 the the DT cord-293913-frkb8iso 57 23 control control NN cord-293913-frkb8iso 57 24 of of IN cord-293913-frkb8iso 57 25 a a DT cord-293913-frkb8iso 57 26 tac tac NNP cord-293913-frkb8iso 57 27 promoter promoter NN cord-293913-frkb8iso 57 28 , , , cord-293913-frkb8iso 57 29 was be VBD cord-293913-frkb8iso 57 30 kindly kindly RB cord-293913-frkb8iso 57 31 provided provide VBN cord-293913-frkb8iso 57 32 by by IN cord-293913-frkb8iso 57 33 Dr. Dr. NNP cord-293913-frkb8iso 57 34 Steven Steven NNP cord-293913-frkb8iso 57 35 Broyles Broyles NNP cord-293913-frkb8iso 57 36 ( ( -LRB- cord-293913-frkb8iso 57 37 Purdue Purdue NNP cord-293913-frkb8iso 57 38 University University NNP cord-293913-frkb8iso 57 39 , , , cord-293913-frkb8iso 57 40 West West NNP cord-293913-frkb8iso 57 41 Lafayette Lafayette NNP cord-293913-frkb8iso 57 42 , , , cord-293913-frkb8iso 57 43 IN in IN cord-293913-frkb8iso 57 44 ) ) -RRB- cord-293913-frkb8iso 57 45 . . . cord-293913-frkb8iso 58 1 To to TO cord-293913-frkb8iso 58 2 generate generate VB cord-293913-frkb8iso 58 3 a a DT cord-293913-frkb8iso 58 4 GST GST NNP cord-293913-frkb8iso 58 5 - - HYPH cord-293913-frkb8iso 58 6 MHV mhv NN cord-293913-frkb8iso 58 7 fusion fusion NN cord-293913-frkb8iso 58 8 protvin protvin NNS cord-293913-frkb8iso 58 9 , , , cord-293913-frkb8iso 58 10 a a DT cord-293913-frkb8iso 58 11 647 647 CD cord-293913-frkb8iso 58 12 basepair basepair NN cord-293913-frkb8iso 58 13 XhoI XhoI NNP cord-293913-frkb8iso 58 14 fragment fragment NN cord-293913-frkb8iso 58 15 ( ( -LRB- cord-293913-frkb8iso 58 16 nt nt RB cord-293913-frkb8iso 58 17 1128 1128 CD cord-293913-frkb8iso 58 18 - - SYM cord-293913-frkb8iso 58 19 1775 1775 CD cord-293913-frkb8iso 58 20 of of IN cord-293913-frkb8iso 58 21 MHV MHV NNP cord-293913-frkb8iso 58 22 JHM JHM NNP cord-293913-frkb8iso 58 23 - - HYPH cord-293913-frkb8iso 58 24 X X NNP cord-293913-frkb8iso 58 25 ) ) -RRB- cord-293913-frkb8iso 58 26 , , , cord-293913-frkb8iso 58 27 was be VBD cord-293913-frkb8iso 58 28 isolated isolate VBN cord-293913-frkb8iso 58 29 from from IN cord-293913-frkb8iso 58 30 pT7-NBgl pt7-nbgl JJ cord-293913-frkb8iso 58 31 plasmid plasmid NN cord-293913-frkb8iso 58 32 DNA DNA NNP cord-293913-frkb8iso 58 33 ( ( -LRB- cord-293913-frkb8iso 58 34 Baker Baker NNP cord-293913-frkb8iso 58 35 et et NNP cord-293913-frkb8iso 58 36 at at IN cord-293913-frkb8iso 58 37 . . . cord-293913-frkb8iso 58 38 , , , cord-293913-frkb8iso 58 39 1989 1989 CD cord-293913-frkb8iso 58 40 ) ) -RRB- cord-293913-frkb8iso 58 41 and and CC cord-293913-frkb8iso 58 42 ligated ligate VBD cord-293913-frkb8iso 58 43 into into IN cord-293913-frkb8iso 58 44 the the DT cord-293913-frkb8iso 58 45 XhoI XhoI NNP cord-293913-frkb8iso 58 46 site site NN cord-293913-frkb8iso 58 47 of of IN cord-293913-frkb8iso 58 48 pGEX pgex JJ cord-293913-frkb8iso 58 49 - - HYPH cord-293913-frkb8iso 58 50 KG kg NN cord-293913-frkb8iso 58 51 . . . cord-293913-frkb8iso 59 1 The the DT cord-293913-frkb8iso 59 2 resulting result VBG cord-293913-frkb8iso 59 3 expression expression NN cord-293913-frkb8iso 59 4 plasmid plasmid NN cord-293913-frkb8iso 59 5 which which WDT cord-293913-frkb8iso 59 6 encodes encode VBZ cord-293913-frkb8iso 59 7 a a DT cord-293913-frkb8iso 59 8 GST GST NNP cord-293913-frkb8iso 59 9 - - HYPH cord-293913-frkb8iso 59 10 MHV mhv NN cord-293913-frkb8iso 59 11 fusion fusion NN cord-293913-frkb8iso 59 12 protein protein NN cord-293913-frkb8iso 59 13 with with IN cord-293913-frkb8iso 59 14 a a DT cord-293913-frkb8iso 59 15 predicted predict VBN cord-293913-frkb8iso 59 16 molec- molec- JJ cord-293913-frkb8iso 59 17 ular ular JJ cord-293913-frkb8iso 59 18 mass mass NN cord-293913-frkb8iso 59 19 of of IN cord-293913-frkb8iso 59 20 50 50 CD cord-293913-frkb8iso 59 21 kDa kDa NNS cord-293913-frkb8iso 59 22 , , , cord-293913-frkb8iso 59 23 was be VBD cord-293913-frkb8iso 59 24 designated designate VBN cord-293913-frkb8iso 59 25 pGEX-647 pGEX-647 VBN cord-293913-frkb8iso 59 26 . . . cord-293913-frkb8iso 60 1 The the DT cord-293913-frkb8iso 60 2 fusion fusion NN cord-293913-frkb8iso 60 3 protein protein NN cord-293913-frkb8iso 60 4 was be VBD cord-293913-frkb8iso 60 5 induced induce VBN cord-293913-frkb8iso 60 6 and and CC cord-293913-frkb8iso 60 7 isolated isolate VBN cord-293913-frkb8iso 60 8 following follow VBG cord-293913-frkb8iso 60 9 the the DT cord-293913-frkb8iso 60 10 method method NN cord-293913-frkb8iso 60 11 of of IN cord-293913-frkb8iso 60 12 Guan Guan NNP cord-293913-frkb8iso 60 13 ( ( -LRB- cord-293913-frkb8iso 60 14 Guan Guan NNP cord-293913-frkb8iso 60 15 and and CC cord-293913-frkb8iso 60 16 Dixon Dixon NNP cord-293913-frkb8iso 60 17 , , , cord-293913-frkb8iso 60 18 1991 1991 CD cord-293913-frkb8iso 60 19 ) ) -RRB- cord-293913-frkb8iso 60 20 , , , cord-293913-frkb8iso 60 21 and and CC cord-293913-frkb8iso 60 22 injected inject VBN cord-293913-frkb8iso 60 23 into into IN cord-293913-frkb8iso 60 24 rabbits rabbit NNS cord-293913-frkb8iso 60 25 to to TO cord-293913-frkb8iso 60 26 generate generate VB cord-293913-frkb8iso 60 27 polyclonal polyclonal JJ cord-293913-frkb8iso 60 28 antiserum antiserum NN cord-293913-frkb8iso 60 29 . . . cord-293913-frkb8iso 61 1 To to TO cord-293913-frkb8iso 61 2 identify identify VB cord-293913-frkb8iso 61 3 MHV MHV NNP cord-293913-frkb8iso 61 4 gene gene NN cord-293913-frkb8iso 61 5 products product NNS cord-293913-frkb8iso 61 6 , , , cord-293913-frkb8iso 61 7 a a DT cord-293913-frkb8iso 61 8 mouse mouse NN cord-293913-frkb8iso 61 9 fibroblast fibroblast NN cord-293913-frkb8iso 61 10 cell cell NN cord-293913-frkb8iso 61 11 line line NN cord-293913-frkb8iso 61 12 , , , cord-293913-frkb8iso 61 13 17C1 17c1 CD cord-293913-frkb8iso 61 14 - - SYM cord-293913-frkb8iso 61 15 1 1 CD cord-293913-frkb8iso 61 16 , , , cord-293913-frkb8iso 61 17 was be VBD cord-293913-frkb8iso 61 18 infected infect VBN cord-293913-frkb8iso 61 19 with with IN cord-293913-frkb8iso 61 20 MHV MHV NNP cord-293913-frkb8iso 61 21 - - HYPH cord-293913-frkb8iso 61 22 JHM JHM NNP cord-293913-frkb8iso 61 23 at at IN cord-293913-frkb8iso 61 24 a a DT cord-293913-frkb8iso 61 25 multiplicity multiplicity NN cord-293913-frkb8iso 61 26 of of IN cord-293913-frkb8iso 61 27 infection infection NN cord-293913-frkb8iso 61 28 ( ( -LRB- cord-293913-frkb8iso 61 29 m.o.i m.o.i NNP cord-293913-frkb8iso 61 30 . . NNP cord-293913-frkb8iso 61 31 ) ) -RRB- cord-293913-frkb8iso 61 32 of of IN cord-293913-frkb8iso 61 33 10 10 CD cord-293913-frkb8iso 61 34 and and CC cord-293913-frkb8iso 61 35 labeled label VBN cord-293913-frkb8iso 61 36 at at IN cord-293913-frkb8iso 61 37 6.5 6.5 CD cord-293913-frkb8iso 61 38 h h NN cord-293913-frkb8iso 61 39 post post NN cord-293913-frkb8iso 61 40 - - NN cord-293913-frkb8iso 61 41 infection infection JJ cord-293913-frkb8iso 61 42 ( ( -LRB- cord-293913-frkb8iso 61 43 p.i p.i NNP cord-293913-frkb8iso 61 44 . . NNP cord-293913-frkb8iso 61 45 ) ) -RRB- cord-293913-frkb8iso 61 46 with with IN cord-293913-frkb8iso 61 47 [ [ -LRB- cord-293913-frkb8iso 61 48 35S]methionine 35s]methionine CD cord-293913-frkb8iso 61 49 for for IN cord-293913-frkb8iso 61 50 120 120 CD cord-293913-frkb8iso 61 51 min min NN cord-293913-frkb8iso 61 52 . . . cord-293913-frkb8iso 62 1 Whole whole JJ cord-293913-frkb8iso 62 2 cell cell NN cord-293913-frkb8iso 62 3 lysates lysate NNS cord-293913-frkb8iso 62 4 were be VBD cord-293913-frkb8iso 62 5 prepared prepare VBN cord-293913-frkb8iso 62 6 and and CC cord-293913-frkb8iso 62 7 immunoprecipitated immunoprecipitate VBN cord-293913-frkb8iso 62 8 as as IN cord-293913-frkb8iso 62 9 previously previously RB cord-293913-frkb8iso 62 10 described describe VBN cord-293913-frkb8iso 62 11 ( ( -LRB- cord-293913-frkb8iso 62 12 Baker Baker NNP cord-293913-frkb8iso 62 13 et et NNP cord-293913-frkb8iso 62 14 al al NNP cord-293913-frkb8iso 62 15 . . NNP cord-293913-frkb8iso 62 16 , , , cord-293913-frkb8iso 62 17 1989 1989 CD cord-293913-frkb8iso 62 18 ) ) -RRB- cord-293913-frkb8iso 62 19 using use VBG cord-293913-frkb8iso 62 20 protein protein NN cord-293913-frkb8iso 62 21 A A NNP cord-293913-frkb8iso 62 22 - - HYPH cord-293913-frkb8iso 62 23 sepharose sepharose JJ cord-293913-frkb8iso 62 24 beads bead NNS cord-293913-frkb8iso 62 25 and and CC cord-293913-frkb8iso 62 26 anti anti JJ cord-293913-frkb8iso 62 27 - - JJ cord-293913-frkb8iso 62 28 p28 p28 JJ cord-293913-frkb8iso 62 29 , , , cord-293913-frkb8iso 62 30 anti-647 anti-647 JJ cord-293913-frkb8iso 62 31 or or CC cord-293913-frkb8iso 62 32 pre pre JJ cord-293913-frkb8iso 62 33 - - JJ cord-293913-frkb8iso 62 34 immune immune JJ cord-293913-frkb8iso 62 35 serum serum NN cord-293913-frkb8iso 62 36 , , , cord-293913-frkb8iso 62 37 and and CC cord-293913-frkb8iso 62 38 analyzed analyze VBN cord-293913-frkb8iso 62 39 by by IN cord-293913-frkb8iso 62 40 electrophoresis electrophoresis NN cord-293913-frkb8iso 62 41 in in IN cord-293913-frkb8iso 62 42 a a DT cord-293913-frkb8iso 62 43 10 10 CD cord-293913-frkb8iso 62 44 % % NN cord-293913-frkb8iso 62 45 SDS sds NN cord-293913-frkb8iso 62 46 - - HYPH cord-293913-frkb8iso 62 47 polyacrylamide polyacrylamide NN cord-293913-frkb8iso 62 48 gel gel NN cord-293913-frkb8iso 62 49 ( ( -LRB- cord-293913-frkb8iso 62 50 Fig fig NN cord-293913-frkb8iso 62 51 . . NNP cord-293913-frkb8iso 62 52 2 2 CD cord-293913-frkb8iso 62 53 ) ) -RRB- cord-293913-frkb8iso 62 54 . . . cord-293913-frkb8iso 63 1 The the DT cord-293913-frkb8iso 63 2 amino amino NN cord-293913-frkb8iso 63 3 - - HYPH cord-293913-frkb8iso 63 4 terminal terminal NN cord-293913-frkb8iso 63 5 product product NN cord-293913-frkb8iso 63 6 of of IN cord-293913-frkb8iso 63 7 the the DT cord-293913-frkb8iso 63 8 polymerase polymerase NN cord-293913-frkb8iso 63 9 polyprotein polyprotein NNP cord-293913-frkb8iso 63 10 , , , cord-293913-frkb8iso 63 11 p28 p28 CD cord-293913-frkb8iso 63 12 , , , cord-293913-frkb8iso 63 13 was be VBD cord-293913-frkb8iso 63 14 detected detect VBN cord-293913-frkb8iso 63 15 in in IN cord-293913-frkb8iso 63 16 MHV MHV NNP cord-293913-frkb8iso 63 17 - - HYPH cord-293913-frkb8iso 63 18 JHM JHM NNP cord-293913-frkb8iso 63 19 infected infected JJ cord-293913-frkb8iso 63 20 cells cell NNS cord-293913-frkb8iso 63 21 using use VBG cord-293913-frkb8iso 63 22 anti anti JJ cord-293913-frkb8iso 63 23 - - JJ cord-293913-frkb8iso 63 24 p28 p28 JJ cord-293913-frkb8iso 63 25 ( ( -LRB- cord-293913-frkb8iso 63 26 Lane Lane NNP cord-293913-frkb8iso 63 27 6 6 CD cord-293913-frkb8iso 63 28 ) ) -RRB- cord-293913-frkb8iso 63 29 , , , cord-293913-frkb8iso 63 30 demonstrating demonstrate VBG cord-293913-frkb8iso 63 31 that that DT cord-293913-frkb8iso 63 32 translation translation NN cord-293913-frkb8iso 63 33 and and CC cord-293913-frkb8iso 63 34 processing processing NN cord-293913-frkb8iso 63 35 of of IN cord-293913-frkb8iso 63 36 the the DT cord-293913-frkb8iso 63 37 polymerase polymerase NN cord-293913-frkb8iso 63 38 polyprotein polyprotein NN cord-293913-frkb8iso 63 39 was be VBD cord-293913-frkb8iso 63 40 occurring occur VBG cord-293913-frkb8iso 63 41 during during IN cord-293913-frkb8iso 63 42 the the DT cord-293913-frkb8iso 63 43 labeling labeling NN cord-293913-frkb8iso 63 44 period period NN cord-293913-frkb8iso 63 45 . . . cord-293913-frkb8iso 64 1 The the DT cord-293913-frkb8iso 64 2 anti-647 anti-647 JJ cord-293913-frkb8iso 64 3 serum serum NN cord-293913-frkb8iso 64 4 specifically specifically RB cord-293913-frkb8iso 64 5 precipitated precipitate VBD cord-293913-frkb8iso 64 6 a a DT cord-293913-frkb8iso 64 7 protein protein NN cord-293913-frkb8iso 64 8 with with IN cord-293913-frkb8iso 64 9 an an DT cord-293913-frkb8iso 64 10 apparent apparent JJ cord-293913-frkb8iso 64 11 molecular molecular JJ cord-293913-frkb8iso 64 12 mass mass NN cord-293913-frkb8iso 64 13 of of IN cord-293913-frkb8iso 64 14 65 65 CD cord-293913-frkb8iso 64 15 kDa kDa NNP cord-293913-frkb8iso 64 16 ( ( -LRB- cord-293913-frkb8iso 64 17 Lane Lane NNP cord-293913-frkb8iso 64 18 4 4 CD cord-293913-frkb8iso 64 19 ) ) -RRB- cord-293913-frkb8iso 64 20 , , , cord-293913-frkb8iso 64 21 which which WDT cord-293913-frkb8iso 64 22 was be VBD cord-293913-frkb8iso 64 23 not not RB cord-293913-frkb8iso 64 24 detected detect VBN cord-293913-frkb8iso 64 25 in in IN cord-293913-frkb8iso 64 26 mock mock JJ cord-293913-frkb8iso 64 27 infected infected JJ cord-293913-frkb8iso 64 28 cells cell NNS cord-293913-frkb8iso 64 29 ( ( -LRB- cord-293913-frkb8iso 64 30 Lane lane NN cord-293913-frkb8iso 64 31 3 3 CD cord-293913-frkb8iso 64 32 ) ) -RRB- cord-293913-frkb8iso 64 33 or or CC cord-293913-frkb8iso 64 34 from from IN cord-293913-frkb8iso 64 35 mock mock JJ cord-293913-frkb8iso 64 36 or or CC cord-293913-frkb8iso 64 37 MHV MHV NNP cord-293913-frkb8iso 64 38 - - HYPH cord-293913-frkb8iso 64 39 JHM JHM NNP cord-293913-frkb8iso 64 40 infected infected JJ cord-293913-frkb8iso 64 41 cell cell NN cord-293913-frkb8iso 64 42 lysates lysate NNS cord-293913-frkb8iso 64 43 immunoprecipitated immunoprecipitate VBN cord-293913-frkb8iso 64 44 with with IN cord-293913-frkb8iso 64 45 pre pre JJ cord-293913-frkb8iso 64 46 - - JJ cord-293913-frkb8iso 64 47 immune immune JJ cord-293913-frkb8iso 64 48 serum serum NN cord-293913-frkb8iso 64 49 ( ( -LRB- cord-293913-frkb8iso 64 50 Lanes lane NNS cord-293913-frkb8iso 64 51 1 1 CD cord-293913-frkb8iso 64 52 and and CC cord-293913-frkb8iso 64 53 2 2 CD cord-293913-frkb8iso 64 54 ) ) -RRB- cord-293913-frkb8iso 64 55 . . . cord-293913-frkb8iso 65 1 Two two CD cord-293913-frkb8iso 65 2 additional additional JJ cord-293913-frkb8iso 65 3 MHV MHV NNP cord-293913-frkb8iso 65 4 - - HYPH cord-293913-frkb8iso 65 5 JHM JHM NNP cord-293913-frkb8iso 65 6 specific specific JJ cord-293913-frkb8iso 65 7 proteins protein NNS cord-293913-frkb8iso 65 8 of of IN cord-293913-frkb8iso 65 9 72 72 CD cord-293913-frkb8iso 65 10 kDa kDa NNS cord-293913-frkb8iso 65 11 and and CC cord-293913-frkb8iso 65 12 greater great JJR cord-293913-frkb8iso 65 13 than than IN cord-293913-frkb8iso 65 14 300 300 CD cord-293913-frkb8iso 65 15 kDa kDa NNS cord-293913-frkb8iso 65 16 were be VBD cord-293913-frkb8iso 65 17 also also RB cord-293913-frkb8iso 65 18 detected detect VBN cord-293913-frkb8iso 65 19 using use VBG cord-293913-frkb8iso 65 20 anti-647 anti-647 JJ cord-293913-frkb8iso 65 21 serum serum NN cord-293913-frkb8iso 65 22 . . . cord-293913-frkb8iso 66 1 To to TO cord-293913-frkb8iso 66 2 determine determine VB cord-293913-frkb8iso 66 3 if if IN cord-293913-frkb8iso 66 4 the the DT cord-293913-frkb8iso 66 5 p72 p72 NN cord-293913-frkb8iso 66 6 and/or and/or CC cord-293913-frkb8iso 66 7 300 300 CD cord-293913-frkb8iso 66 8 kDa kDa NNS cord-293913-frkb8iso 66 9 proteins protein NNS cord-293913-frkb8iso 66 10 were be VBD cord-293913-frkb8iso 66 11 precursors precursor NNS cord-293913-frkb8iso 66 12 to to IN cord-293913-frkb8iso 66 13 p65 p65 ADD cord-293913-frkb8iso 66 14 , , , cord-293913-frkb8iso 66 15 we -PRON- PRP cord-293913-frkb8iso 66 16 performed perform VBD cord-293913-frkb8iso 66 17 pulse pulse NN cord-293913-frkb8iso 66 18 and and CC cord-293913-frkb8iso 66 19 chase chase NN cord-293913-frkb8iso 66 20 experiments experiment NNS cord-293913-frkb8iso 66 21 . . . cord-293913-frkb8iso 67 1 MHV MHV NNP cord-293913-frkb8iso 67 2 - - HYPH cord-293913-frkb8iso 67 3 JHM JHM NNP cord-293913-frkb8iso 67 4 infected infected JJ cord-293913-frkb8iso 67 5 cells cell NNS cord-293913-frkb8iso 67 6 were be VBD cord-293913-frkb8iso 67 7 pulse pulse NN cord-293913-frkb8iso 67 8 - - HYPH cord-293913-frkb8iso 67 9 labeled label VBN cord-293913-frkb8iso 67 10 with with IN cord-293913-frkb8iso 67 11 [ [ -LRB- cord-293913-frkb8iso 67 12 35S]methionine 35s]methionine CD cord-293913-frkb8iso 67 13 from from IN cord-293913-frkb8iso 67 14 7.0 7.0 CD cord-293913-frkb8iso 67 15 to to IN cord-293913-frkb8iso 67 16 7.5 7.5 CD cord-293913-frkb8iso 67 17 h h NN cord-293913-frkb8iso 67 18 p.i p.i NNP cord-293913-frkb8iso 67 19 . . . cord-293913-frkb8iso 68 1 Following follow VBG cord-293913-frkb8iso 68 2 the the DT cord-293913-frkb8iso 68 3 pulse pulse NN cord-293913-frkb8iso 68 4 , , , cord-293913-frkb8iso 68 5 the the DT cord-293913-frkb8iso 68 6 radiolabeled radiolabele VBN cord-293913-frkb8iso 68 7 proteins protein NNS cord-293913-frkb8iso 68 8 were be VBD cord-293913-frkb8iso 68 9 chased chase VBN cord-293913-frkb8iso 68 10 by by IN cord-293913-frkb8iso 68 11 replacing replace VBG cord-293913-frkb8iso 68 12 the the DT cord-293913-frkb8iso 68 13 labeling labeling NN cord-293913-frkb8iso 68 14 media medium NNS cord-293913-frkb8iso 68 15 with with IN cord-293913-frkb8iso 68 16 media medium NNS cord-293913-frkb8iso 68 17 containing contain VBG cord-293913-frkb8iso 68 18 excess excess JJ cord-293913-frkb8iso 68 19 unlabeled unlabeled JJ cord-293913-frkb8iso 68 20 methionine methionine NN cord-293913-frkb8iso 68 21 . . . cord-293913-frkb8iso 69 1 Whole whole JJ cord-293913-frkb8iso 69 2 cell cell NN cord-293913-frkb8iso 69 3 lysates lysate NNS cord-293913-frkb8iso 69 4 were be VBD cord-293913-frkb8iso 69 5 prepared prepare VBN cord-293913-frkb8iso 69 6 at at IN cord-293913-frkb8iso 69 7 various various JJ cord-293913-frkb8iso 69 8 times time NNS cord-293913-frkb8iso 69 9 during during IN cord-293913-frkb8iso 69 10 the the DT cord-293913-frkb8iso 69 11 chase chase NN cord-293913-frkb8iso 69 12 and and CC cord-293913-frkb8iso 69 13 immunoprecipitated immunoprecipitate VBD cord-293913-frkb8iso 69 14 with with IN cord-293913-frkb8iso 69 15 anti anti JJ cord-293913-frkb8iso 69 16 - - JJ cord-293913-frkb8iso 69 17 p28 p28 JJ cord-293913-frkb8iso 69 18 and and CC cord-293913-frkb8iso 69 19 anti-647 anti-647 NNP cord-293913-frkb8iso 69 20 , , , cord-293913-frkb8iso 69 21 and and CC cord-293913-frkb8iso 69 22 products product NNS cord-293913-frkb8iso 69 23 were be VBD cord-293913-frkb8iso 69 24 analyzed analyze VBN cord-293913-frkb8iso 69 25 by by IN cord-293913-frkb8iso 69 26 electrophoresis electrophoresis NN cord-293913-frkb8iso 69 27 in in IN cord-293913-frkb8iso 69 28 10 10 CD cord-293913-frkb8iso 69 29 % % NN cord-293913-frkb8iso 69 30 SDSpolyacrylamide sdspolyacrylamide JJ cord-293913-frkb8iso 69 31 gels gel NNS cord-293913-frkb8iso 69 32 ( ( -LRB- cord-293913-frkb8iso 69 33 Fig Fig NNP cord-293913-frkb8iso 69 34 . . NNP cord-293913-frkb8iso 69 35 3(A 3(a CD cord-293913-frkb8iso 69 36 , , , cord-293913-frkb8iso 69 37 B B NNP cord-293913-frkb8iso 69 38 ) ) -RRB- cord-293913-frkb8iso 69 39 ) ) -RRB- cord-293913-frkb8iso 69 40 . . . cord-293913-frkb8iso 70 1 The the DT cord-293913-frkb8iso 70 2 p28 p28 NNP cord-293913-frkb8iso 70 3 protein protein NN cord-293913-frkb8iso 70 4 is be VBZ cord-293913-frkb8iso 70 5 detected detect VBN cord-293913-frkb8iso 70 6 immediately immediately RB cord-293913-frkb8iso 70 7 after after IN cord-293913-frkb8iso 70 8 the the DT cord-293913-frkb8iso 70 9 30 30 CD cord-293913-frkb8iso 70 10 min min NN cord-293913-frkb8iso 70 11 pulse pulse NN cord-293913-frkb8iso 70 12 and and CC cord-293913-frkb8iso 70 13 no no DT cord-293913-frkb8iso 70 14 precursor precursor NN cord-293913-frkb8iso 70 15 protein protein NN cord-293913-frkb8iso 70 16 is be VBZ cord-293913-frkb8iso 70 17 observed observe VBN cord-293913-frkb8iso 70 18 ( ( -LRB- cord-293913-frkb8iso 70 19 Fig Fig NNP cord-293913-frkb8iso 70 20 . . . cord-293913-frkb8iso 70 21 _SP cord-293913-frkb8iso 70 22 3(A 3(a CD cord-293913-frkb8iso 70 23 ) ) -RRB- cord-293913-frkb8iso 70 24 ) ) -RRB- cord-293913-frkb8iso 70 25 . . . cord-293913-frkb8iso 71 1 The the DT cord-293913-frkb8iso 71 2 p28 p28 NNP cord-293913-frkb8iso 71 3 protein protein NN cord-293913-frkb8iso 71 4 also also RB cord-293913-frkb8iso 71 5 appears appear VBZ cord-293913-frkb8iso 71 6 to to TO cord-293913-frkb8iso 71 7 be be VB cord-293913-frkb8iso 71 8 stable stable JJ cord-293913-frkb8iso 71 9 throughout throughout IN cord-293913-frkb8iso 71 10 the the DT cord-293913-frkb8iso 71 11 120 120 CD cord-293913-frkb8iso 71 12 min min NN cord-293913-frkb8iso 71 13 chase chase NN cord-293913-frkb8iso 71 14 . . . cord-293913-frkb8iso 72 1 This this DT cord-293913-frkb8iso 72 2 result result NN cord-293913-frkb8iso 72 3 is be VBZ cord-293913-frkb8iso 72 4 consistent consistent JJ cord-293913-frkb8iso 72 5 with with IN cord-293913-frkb8iso 72 6 previous previous JJ cord-293913-frkb8iso 72 7 data datum NNS cord-293913-frkb8iso 72 8 demonstrating demonstrate VBG cord-293913-frkb8iso 72 9 that that IN cord-293913-frkb8iso 72 10 p28 p28 NN cord-293913-frkb8iso 72 11 is be VBZ cord-293913-frkb8iso 72 12 cleaved cleave VBN cord-293913-frkb8iso 72 13 in in IN cord-293913-frkb8iso 72 14 cis cis NNP cord-293913-frkb8iso 72 15 and and CC cord-293913-frkb8iso 72 16 is be VBZ cord-293913-frkb8iso 72 17 stable stable JJ cord-293913-frkb8iso 72 18 in in IN cord-293913-frkb8iso 72 19 MHV mhv NN cord-293913-frkb8iso 72 20 - - HYPH cord-293913-frkb8iso 72 21 infected infect VBN cord-293913-frkb8iso 72 22 cells cell NNS cord-293913-frkb8iso 72 23 ( ( -LRB- cord-293913-frkb8iso 72 24 Denison Denison NNP cord-293913-frkb8iso 72 25 and and CC cord-293913-frkb8iso 72 26 Perlman Perlman NNP cord-293913-frkb8iso 72 27 , , , cord-293913-frkb8iso 72 28 1987 1987 CD cord-293913-frkb8iso 72 29 ; ; : cord-293913-frkb8iso 72 30 Baker Baker NNP cord-293913-frkb8iso 72 31 et et NNP cord-293913-frkb8iso 72 32 al al NNP cord-293913-frkb8iso 72 33 . . NNP cord-293913-frkb8iso 72 34 , , , cord-293913-frkb8iso 72 35 1989 1989 CD cord-293913-frkb8iso 72 36 ) ) -RRB- cord-293913-frkb8iso 72 37 . . . cord-293913-frkb8iso 73 1 Immunoprecipitation immunoprecipitation NN cord-293913-frkb8iso 73 2 of of IN cord-293913-frkb8iso 73 3 the the DT cord-293913-frkb8iso 73 4 MHV MHV NNP cord-293913-frkb8iso 73 5 - - HYPH cord-293913-frkb8iso 73 6 JHM JHM NNP cord-293913-frkb8iso 73 7 infected infected JJ cord-293913-frkb8iso 73 8 lysates lysate NNS cord-293913-frkb8iso 73 9 with with IN cord-293913-frkb8iso 73 10 anti-647 anti-647 NNP cord-293913-frkb8iso 73 11 reveals reveal VBZ cord-293913-frkb8iso 73 12 a a DT cord-293913-frkb8iso 73 13 precursorproduct precursorproduct JJ cord-293913-frkb8iso 73 14 relationship relationship NN cord-293913-frkb8iso 73 15 of of IN cord-293913-frkb8iso 73 16 p72 p72 NN cord-293913-frkb8iso 73 17 and and CC cord-293913-frkb8iso 73 18 p65 p65 NN cord-293913-frkb8iso 73 19 ( ( -LRB- cord-293913-frkb8iso 73 20 Fig fig NN cord-293913-frkb8iso 73 21 . . . cord-293913-frkb8iso 74 1 3(B 3(b LS cord-293913-frkb8iso 74 2 ) ) -RRB- cord-293913-frkb8iso 74 3 ) ) -RRB- cord-293913-frkb8iso 74 4 . . . cord-293913-frkb8iso 75 1 The the DT cord-293913-frkb8iso 75 2 p72 p72 NN cord-293913-frkb8iso 75 3 protein protein NN cord-293913-frkb8iso 75 4 is be VBZ cord-293913-frkb8iso 75 5 the the DT cord-293913-frkb8iso 75 6 predominant predominant JJ cord-293913-frkb8iso 75 7 viral viral JJ cord-293913-frkb8iso 75 8 protein protein NN cord-293913-frkb8iso 75 9 precipitated precipitate VBN cord-293913-frkb8iso 75 10 from from IN cord-293913-frkb8iso 75 11 the the DT cord-293913-frkb8iso 75 12 pulse pulse NN cord-293913-frkb8iso 75 13 - - HYPH cord-293913-frkb8iso 75 14 labeled label VBN cord-293913-frkb8iso 75 15 cells cell NNS cord-293913-frkb8iso 75 16 . . . cord-293913-frkb8iso 76 1 The the DT cord-293913-frkb8iso 76 2 p65 p65 NN cord-293913-frkb8iso 76 3 protein protein NN cord-293913-frkb8iso 76 4 is be VBZ cord-293913-frkb8iso 76 5 detected detect VBN cord-293913-frkb8iso 76 6 by by IN cord-293913-frkb8iso 76 7 15 15 CD cord-293913-frkb8iso 76 8 min min NN cord-293913-frkb8iso 76 9 of of IN cord-293913-frkb8iso 76 10 chase chase NN cord-293913-frkb8iso 76 11 and and CC cord-293913-frkb8iso 76 12 gradually gradually RB cord-293913-frkb8iso 76 13 accumulates accumulate VBZ cord-293913-frkb8iso 76 14 with with IN cord-293913-frkb8iso 76 15 a a DT cord-293913-frkb8iso 76 16 concomitant concomitant JJ cord-293913-frkb8iso 76 17 decrease decrease NN cord-293913-frkb8iso 76 18 in in IN cord-293913-frkb8iso 76 19 p72 p72 NN cord-293913-frkb8iso 76 20 . . . cord-293913-frkb8iso 77 1 These these DT cord-293913-frkb8iso 77 2 results result NNS cord-293913-frkb8iso 77 3 indicate indicate VBP cord-293913-frkb8iso 77 4 that that IN cord-293913-frkb8iso 77 5 the the DT cord-293913-frkb8iso 77 6 p72 p72 NN cord-293913-frkb8iso 77 7 is be VBZ cord-293913-frkb8iso 77 8 likely likely RB cord-293913-frkb8iso 77 9 the the DT cord-293913-frkb8iso 77 10 precursor precursor NN cord-293913-frkb8iso 77 11 to to IN cord-293913-frkb8iso 77 12 the the DT cord-293913-frkb8iso 77 13 p65 p65 NN cord-293913-frkb8iso 77 14 protein protein NN cord-293913-frkb8iso 77 15 . . . cord-293913-frkb8iso 78 1 The the DT cord-293913-frkb8iso 78 2 p72 p72 NN cord-293913-frkb8iso 78 3 and and CC cord-293913-frkb8iso 78 4 p65 p65 NN cord-293913-frkb8iso 78 5 proteins protein NNS cord-293913-frkb8iso 78 6 may may MD cord-293913-frkb8iso 78 7 share share VB cord-293913-frkb8iso 78 8 either either CC cord-293913-frkb8iso 78 9 amino amino NN cord-293913-frkb8iso 78 10 - - HYPH cord-293913-frkb8iso 78 11 or or CC cord-293913-frkb8iso 78 12 carboxy carboxy JJ cord-293913-frkb8iso 78 13 - - HYPH cord-293913-frkb8iso 78 14 terminal terminal NN cord-293913-frkb8iso 78 15 regions region NNS cord-293913-frkb8iso 78 16 and and CC cord-293913-frkb8iso 78 17 be be VB cord-293913-frkb8iso 78 18 recognized recognize VBN cord-293913-frkb8iso 78 19 by by IN cord-293913-frkb8iso 78 20 the the DT cord-293913-frkb8iso 78 21 anti-647 anti-647 NNP cord-293913-frkb8iso 78 22 serum serum NN cord-293913-frkb8iso 78 23 . . . cord-293913-frkb8iso 79 1 The the DT cord-293913-frkb8iso 79 2 high high JJ cord-293913-frkb8iso 79 3 molecular molecular JJ cord-293913-frkb8iso 79 4 weight weight NN cord-293913-frkb8iso 79 5 protein protein NN cord-293913-frkb8iso 79 6 ( ( -LRB- cord-293913-frkb8iso 79 7 greater great JJR cord-293913-frkb8iso 79 8 than than IN cord-293913-frkb8iso 79 9 300 300 CD cord-293913-frkb8iso 79 10 kDa kDa NNS cord-293913-frkb8iso 79 11 ) ) -RRB- cord-293913-frkb8iso 79 12 was be VBD cord-293913-frkb8iso 79 13 also also RB cord-293913-frkb8iso 79 14 detected detect VBN cord-293913-frkb8iso 79 15 at at IN cord-293913-frkb8iso 79 16 very very RB cord-293913-frkb8iso 79 17 low low JJ cord-293913-frkb8iso 79 18 levels level NNS cord-293913-frkb8iso 79 19 in in IN cord-293913-frkb8iso 79 20 the the DT cord-293913-frkb8iso 79 21 pulse pulse NN cord-293913-frkb8iso 79 22 - - HYPH cord-293913-frkb8iso 79 23 chase chase NN cord-293913-frkb8iso 79 24 experiment experiment NN cord-293913-frkb8iso 79 25 . . . cord-293913-frkb8iso 80 1 This this DT cord-293913-frkb8iso 80 2 large large JJ cord-293913-frkb8iso 80 3 protein protein NN cord-293913-frkb8iso 80 4 likely likely RB cord-293913-frkb8iso 80 5 represents represent VBZ cord-293913-frkb8iso 80 6 a a DT cord-293913-frkb8iso 80 7 primary primary JJ cord-293913-frkb8iso 80 8 translation translation NN cord-293913-frkb8iso 80 9 product product NN cord-293913-frkb8iso 80 10 of of IN cord-293913-frkb8iso 80 11 ORF ORF NNP cord-293913-frkb8iso 80 12 la la NNP cord-293913-frkb8iso 80 13 . . . cord-293913-frkb8iso 81 1 This this DT cord-293913-frkb8iso 81 2 precursor precursor NN cord-293913-frkb8iso 81 3 polyprotein polyprotein NN cord-293913-frkb8iso 81 4 may may MD cord-293913-frkb8iso 81 5 be be VB cord-293913-frkb8iso 81 6 cleaved cleave VBN cord-293913-frkb8iso 81 7 to to TO cord-293913-frkb8iso 81 8 produce produce VB cord-293913-frkb8iso 81 9 p72 p72 NN cord-293913-frkb8iso 81 10 / / SYM cord-293913-frkb8iso 81 11 p65 p65 NN cord-293913-frkb8iso 81 12 and and CC cord-293913-frkb8iso 81 13 the the DT cord-293913-frkb8iso 81 14 p290 p290 NNP cord-293913-frkb8iso 81 15 which which WDT cord-293913-frkb8iso 81 16 was be VBD cord-293913-frkb8iso 81 17 detected detect VBN cord-293913-frkb8iso 81 18 by by IN cord-293913-frkb8iso 81 19 Denison Denison NNP cord-293913-frkb8iso 81 20 and and CC cord-293913-frkb8iso 81 21 co co NNS cord-293913-frkb8iso 81 22 - - NNS cord-293913-frkb8iso 81 23 workers worker NNS cord-293913-frkb8iso 81 24 ( ( -LRB- cord-293913-frkb8iso 81 25 Denison Denison NNP cord-293913-frkb8iso 81 26 et et NNP cord-293913-frkb8iso 81 27 al al NNP cord-293913-frkb8iso 81 28 . . NNP cord-293913-frkb8iso 81 29 , , , cord-293913-frkb8iso 81 30 1992 1992 CD cord-293913-frkb8iso 81 31 ) ) -RRB- cord-293913-frkb8iso 81 32 . . . cord-293913-frkb8iso 82 1 Analysis analysis NN cord-293913-frkb8iso 82 2 of of IN cord-293913-frkb8iso 82 3 the the DT cord-293913-frkb8iso 82 4 MHV MHV NNP cord-293913-frkb8iso 82 5 strain strain NN cord-293913-frkb8iso 82 6 A59 A59 NNP cord-293913-frkb8iso 82 7 polymerase polymerase NN cord-293913-frkb8iso 82 8 gene gene NN cord-293913-frkb8iso 82 9 products product NNS cord-293913-frkb8iso 82 10 has have VBZ cord-293913-frkb8iso 82 11 also also RB cord-293913-frkb8iso 82 12 identified identify VBN cord-293913-frkb8iso 82 13 a a DT cord-293913-frkb8iso 82 14 65 65 CD cord-293913-frkb8iso 82 15 kDa kda NN cord-293913-frkb8iso 82 16 protein protein NN cord-293913-frkb8iso 82 17 as as IN cord-293913-frkb8iso 82 18 the the DT cord-293913-frkb8iso 82 19 mature mature JJ cord-293913-frkb8iso 82 20 proteolytic proteolytic JJ cord-293913-frkb8iso 82 21 product product NN cord-293913-frkb8iso 82 22 adjacent adjacent JJ cord-293913-frkb8iso 82 23 to to IN cord-293913-frkb8iso 82 24 p28 p28 CD cord-293913-frkb8iso 82 25 . . . cord-293913-frkb8iso 83 1 The the DT cord-293913-frkb8iso 83 2 MHV MHV NNP cord-293913-frkb8iso 83 3 - - HYPH cord-293913-frkb8iso 83 4 A59 A59 NNP cord-293913-frkb8iso 83 5 p65 p65 NN cord-293913-frkb8iso 83 6 protein protein NN cord-293913-frkb8iso 83 7 was be VBD cord-293913-frkb8iso 83 8 identified identify VBN cord-293913-frkb8iso 83 9 using use VBG cord-293913-frkb8iso 83 10 a a DT cord-293913-frkb8iso 83 11 fusion fusion NN cord-293913-frkb8iso 83 12 protein protein NN cord-293913-frkb8iso 83 13 antiserum antiserum NN cord-293913-frkb8iso 83 14 ( ( -LRB- cord-293913-frkb8iso 83 15 UP102 UP102 NNP cord-293913-frkb8iso 83 16 ) ) -RRB- cord-293913-frkb8iso 83 17 that that WDT cord-293913-frkb8iso 83 18 was be VBD cord-293913-frkb8iso 83 19 generated generate VBN cord-293913-frkb8iso 83 20 against against IN cord-293913-frkb8iso 83 21 the the DT cord-293913-frkb8iso 83 22 entire entire JJ cord-293913-frkb8iso 83 23 p28 p28 CD cord-293913-frkb8iso 83 24 coding code VBG cord-293913-frkb8iso 83 25 region region NN cord-293913-frkb8iso 83 26 and and CC cord-293913-frkb8iso 83 27 approximately approximately RB cord-293913-frkb8iso 83 28 1 1 CD cord-293913-frkb8iso 83 29 kb kb CD cord-293913-frkb8iso 83 30 of of IN cord-293913-frkb8iso 83 31 adjacent adjacent JJ cord-293913-frkb8iso 83 32 sequence sequence NN cord-293913-frkb8iso 83 33 . . . cord-293913-frkb8iso 84 1 Consistent consistent JJ cord-293913-frkb8iso 84 2 with with IN cord-293913-frkb8iso 84 3 our -PRON- PRP$ cord-293913-frkb8iso 84 4 results result NNS cord-293913-frkb8iso 84 5 , , , cord-293913-frkb8iso 84 6 Denison Denison NNP cord-293913-frkb8iso 84 7 and and CC cord-293913-frkb8iso 84 8 co co NNS cord-293913-frkb8iso 84 9 - - NNS cord-293913-frkb8iso 84 10 workers worker NNS cord-293913-frkb8iso 84 11 demonstrated demonstrate VBD cord-293913-frkb8iso 84 12 that that IN cord-293913-frkb8iso 84 13 p28 p28 NN cord-293913-frkb8iso 84 14 is be VBZ cord-293913-frkb8iso 84 15 detected detect VBN cord-293913-frkb8iso 84 16 immediately immediately RB cord-293913-frkb8iso 84 17 after after IN cord-293913-frkb8iso 84 18 a a DT cord-293913-frkb8iso 84 19 30 30 CD cord-293913-frkb8iso 84 20 min min NN cord-293913-frkb8iso 84 21 pulse pulse NN cord-293913-frkb8iso 84 22 , , , cord-293913-frkb8iso 84 23 and and CC cord-293913-frkb8iso 84 24 that that IN cord-293913-frkb8iso 84 25 p65 p65 NN cord-293913-frkb8iso 84 26 is be VBZ cord-293913-frkb8iso 84 27 detected detect VBN cord-293913-frkb8iso 84 28 after after IN cord-293913-frkb8iso 84 29 approximately approximately RB cord-293913-frkb8iso 84 30 20 20 CD cord-293913-frkb8iso 84 31 min min NN cord-293913-frkb8iso 84 32 of of IN cord-293913-frkb8iso 84 33 chase chase NN cord-293913-frkb8iso 84 34 . . . cord-293913-frkb8iso 85 1 However however RB cord-293913-frkb8iso 85 2 , , , cord-293913-frkb8iso 85 3 no no DT cord-293913-frkb8iso 85 4 precursor precursor NN cord-293913-frkb8iso 85 5 protein protein NN cord-293913-frkb8iso 85 6 of of IN cord-293913-frkb8iso 85 7 72 72 CD cord-293913-frkb8iso 85 8 kDa kDa NNS cord-293913-frkb8iso 85 9 was be VBD cord-293913-frkb8iso 85 10 detected detect VBN cord-293913-frkb8iso 85 11 from from IN cord-293913-frkb8iso 85 12 MHV MHV NNP cord-293913-frkb8iso 85 13 - - HYPH cord-293913-frkb8iso 85 14 A59 A59 NNP cord-293913-frkb8iso 85 15 infected infected JJ cord-293913-frkb8iso 85 16 cells cell NNS cord-293913-frkb8iso 85 17 using use VBG cord-293913-frkb8iso 85 18 antiserum antiserum NN cord-293913-frkb8iso 85 19 UP102 UP102 NNP cord-293913-frkb8iso 85 20 . . . cord-293913-frkb8iso 86 1 There there EX cord-293913-frkb8iso 86 2 are be VBP cord-293913-frkb8iso 86 3 several several JJ cord-293913-frkb8iso 86 4 possible possible JJ cord-293913-frkb8iso 86 5 explanations explanation NNS cord-293913-frkb8iso 86 6 for for IN cord-293913-frkb8iso 86 7 this this DT cord-293913-frkb8iso 86 8 difference difference NN cord-293913-frkb8iso 86 9 . . . cord-293913-frkb8iso 87 1 First first RB cord-293913-frkb8iso 87 2 , , , cord-293913-frkb8iso 87 3 because because IN cord-293913-frkb8iso 87 4 the the DT cord-293913-frkb8iso 87 5 UP102 UP102 NNP cord-293913-frkb8iso 87 6 and and CC cord-293913-frkb8iso 87 7 647 647 CD cord-293913-frkb8iso 87 8 antisera antisera NN cord-293913-frkb8iso 87 9 were be VBD cord-293913-frkb8iso 87 10 generated generate VBN cord-293913-frkb8iso 87 11 against against IN cord-293913-frkb8iso 87 12 different different JJ cord-293913-frkb8iso 87 13 virus virus NN cord-293913-frkb8iso 87 14 strains strain NNS cord-293913-frkb8iso 87 15 and and CC cord-293913-frkb8iso 87 16 in in IN cord-293913-frkb8iso 87 17 different different JJ cord-293913-frkb8iso 87 18 fusion fusion NN cord-293913-frkb8iso 87 19 protein protein NN cord-293913-frkb8iso 87 20 constructs construct NNS cord-293913-frkb8iso 87 21 , , , cord-293913-frkb8iso 87 22 the the DT cord-293913-frkb8iso 87 23 antisera antisera NN cord-293913-frkb8iso 87 24 may may MD cord-293913-frkb8iso 87 25 recognize recognize VB cord-293913-frkb8iso 87 26 different different JJ cord-293913-frkb8iso 87 27 epitopes epitope NNS cord-293913-frkb8iso 87 28 . . . cord-293913-frkb8iso 88 1 The the DT cord-293913-frkb8iso 88 2 p72 p72 NN cord-293913-frkb8iso 88 3 precursor precursor NN cord-293913-frkb8iso 88 4 protein protein NN cord-293913-frkb8iso 88 5 may may MD cord-293913-frkb8iso 88 6 be be VB cord-293913-frkb8iso 88 7 folded fold VBN cord-293913-frkb8iso 88 8 in in IN cord-293913-frkb8iso 88 9 a a DT cord-293913-frkb8iso 88 10 conformation conformation NN cord-293913-frkb8iso 88 11 that that WDT cord-293913-frkb8iso 88 12 is be VBZ cord-293913-frkb8iso 88 13 not not RB cord-293913-frkb8iso 88 14 efficiently efficiently RB cord-293913-frkb8iso 88 15 recognized recognize VBN cord-293913-frkb8iso 88 16 by by IN cord-293913-frkb8iso 88 17 the the DT cord-293913-frkb8iso 88 18 UP102 UP102 NNP cord-293913-frkb8iso 88 19 antiserum antiserum NN cord-293913-frkb8iso 88 20 . . . cord-293913-frkb8iso 89 1 A a DT cord-293913-frkb8iso 89 2 second second JJ cord-293913-frkb8iso 89 3 possible possible JJ cord-293913-frkb8iso 89 4 explanation explanation NN cord-293913-frkb8iso 89 5 is be VBZ cord-293913-frkb8iso 89 6 that that IN cord-293913-frkb8iso 89 7 the the DT cord-293913-frkb8iso 89 8 proteolytic proteolytic JJ cord-293913-frkb8iso 89 9 processing processing NN cord-293913-frkb8iso 89 10 of of IN cord-293913-frkb8iso 89 11 the the DT cord-293913-frkb8iso 89 12 MHV MHV NNP cord-293913-frkb8iso 89 13 - - HYPH cord-293913-frkb8iso 89 14 A59 A59 NNP cord-293913-frkb8iso 89 15 strain strain NN cord-293913-frkb8iso 89 16 and and CC cord-293913-frkb8iso 89 17 the the DT cord-293913-frkb8iso 89 18 MHV MHV NNP cord-293913-frkb8iso 89 19 - - HYPH cord-293913-frkb8iso 89 20 JHM JHM NNP cord-293913-frkb8iso 89 21 strain strain NN cord-293913-frkb8iso 89 22 may may MD cord-293913-frkb8iso 89 23 differ differ VB cord-293913-frkb8iso 89 24 in in IN cord-293913-frkb8iso 89 25 this this DT cord-293913-frkb8iso 89 26 region region NN cord-293913-frkb8iso 89 27 . . . cord-293913-frkb8iso 90 1 To to TO cord-293913-frkb8iso 90 2 determine determine VB cord-293913-frkb8iso 90 3 how how WRB cord-293913-frkb8iso 90 4 p72 p72 NN cord-293913-frkb8iso 90 5 and and CC cord-293913-frkb8iso 90 6 p65 p65 NN cord-293913-frkb8iso 90 7 are be VBP cord-293913-frkb8iso 90 8 generated generate VBN cord-293913-frkb8iso 90 9 from from IN cord-293913-frkb8iso 90 10 the the DT cord-293913-frkb8iso 90 11 polymerase polymerase NN cord-293913-frkb8iso 90 12 polyprotein polyprotein NN cord-293913-frkb8iso 90 13 , , , cord-293913-frkb8iso 90 14 we -PRON- PRP cord-293913-frkb8iso 90 15 expressed express VBD cord-293913-frkb8iso 90 16 cDNA cdna NN cord-293913-frkb8iso 90 17 clones clone NNS cord-293913-frkb8iso 90 18 encoding encode VBG cord-293913-frkb8iso 90 19 the the DT cord-293913-frkb8iso 90 20 5'-end 5'-end CD cord-293913-frkb8iso 90 21 of of IN cord-293913-frkb8iso 91 1 ORF ORF NNP cord-293913-frkb8iso 91 2 l l NNP cord-293913-frkb8iso 91 3 a a NNP cord-293913-frkb8iso 91 4 , , , cord-293913-frkb8iso 91 5 including include VBG cord-293913-frkb8iso 91 6 the the DT cord-293913-frkb8iso 91 7 2 2 CD cord-293913-frkb8iso 91 8 papain papain NN cord-293913-frkb8iso 91 9 - - HYPH cord-293913-frkb8iso 91 10 like like JJ cord-293913-frkb8iso 91 11 cysteine cysteine NN cord-293913-frkb8iso 91 12 proteinase proteinase NN cord-293913-frkb8iso 91 13 domains domain NNS cord-293913-frkb8iso 91 14 , , , cord-293913-frkb8iso 91 15 and and CC cord-293913-frkb8iso 91 16 analyzed analyze VBD cord-293913-frkb8iso 91 17 the the DT cord-293913-frkb8iso 91 18 translation translation NN cord-293913-frkb8iso 91 19 reactions reaction NNS cord-293913-frkb8iso 91 20 for for IN cord-293913-frkb8iso 91 21 the the DT cord-293913-frkb8iso 91 22 presence presence NN cord-293913-frkb8iso 91 23 of of IN cord-293913-frkb8iso 91 24 proteolytic proteolytic JJ cord-293913-frkb8iso 91 25 products product NNS cord-293913-frkb8iso 91 26 . . . cord-293913-frkb8iso 92 1 The the DT cord-293913-frkb8iso 92 2 cDNA cdna NN cord-293913-frkb8iso 92 3 clones clone NNS cord-293913-frkb8iso 92 4 pT7-NA6 pt7-na6 JJ cord-293913-frkb8iso 92 5 and and CC cord-293913-frkb8iso 92 6 pT7-NBgl pT7-NBgl NNP cord-293913-frkb8iso 92 7 , , , cord-293913-frkb8iso 92 8 representing represent VBG cord-293913-frkb8iso 92 9 the the DT cord-293913-frkb8iso 92 10 5'-end 5'-end CD cord-293913-frkb8iso 92 11 6.8 6.8 CD cord-293913-frkb8iso 92 12 and and CC cord-293913-frkb8iso 92 13 5.3 5.3 CD cord-293913-frkb8iso 92 14 kb kb NNS cord-293913-frkb8iso 92 15 , , , cord-293913-frkb8iso 92 16 respectively respectively RB cord-293913-frkb8iso 92 17 ( ( -LRB- cord-293913-frkb8iso 92 18 see see VB cord-293913-frkb8iso 92 19 Fig Fig NNP cord-293913-frkb8iso 92 20 . . NNP cord-293913-frkb8iso 92 21 1 1 CD cord-293913-frkb8iso 92 22 ; ; : cord-293913-frkb8iso 92 23 Baker Baker NNP cord-293913-frkb8iso 92 24 et et NNP cord-293913-frkb8iso 92 25 al al NNP cord-293913-frkb8iso 92 26 . . NNP cord-293913-frkb8iso 92 27 , , , cord-293913-frkb8iso 92 28 1989 1989 CD cord-293913-frkb8iso 92 29 ; ; : cord-293913-frkb8iso 92 30 Lee Lee NNP cord-293913-frkb8iso 92 31 et et NNP cord-293913-frkb8iso 92 32 al al NNP cord-293913-frkb8iso 92 33 . . NNP cord-293913-frkb8iso 92 34 , , , cord-293913-frkb8iso 92 35 1991 1991 CD cord-293913-frkb8iso 92 36 ) ) -RRB- cord-293913-frkb8iso 92 37 , , , cord-293913-frkb8iso 92 38 were be VBD cord-293913-frkb8iso 92 39 expressed express VBN cord-293913-frkb8iso 92 40 in in IN cord-293913-frkb8iso 92 41 vitro vitro FW cord-293913-frkb8iso 92 42 in in IN cord-293913-frkb8iso 92 43 the the DT cord-293913-frkb8iso 92 44 presence presence NN cord-293913-frkb8iso 92 45 of of IN cord-293913-frkb8iso 92 46 [ [ -LRB- cord-293913-frkb8iso 92 47 35S]methionine 35s]methionine CD cord-293913-frkb8iso 92 48 in in IN cord-293913-frkb8iso 92 49 a a DT cord-293913-frkb8iso 92 50 coupled coupled JJ cord-293913-frkb8iso 92 51 transcription transcription NN cord-293913-frkb8iso 92 52 / / SYM cord-293913-frkb8iso 92 53 translation translation NN cord-293913-frkb8iso 92 54 system system NN cord-293913-frkb8iso 92 55 according accord VBG cord-293913-frkb8iso 92 56 to to IN cord-293913-frkb8iso 92 57 the the DT cord-293913-frkb8iso 92 58 manufacturer manufacturer NN cord-293913-frkb8iso 92 59 's 's POS cord-293913-frkb8iso 92 60 instructions instruction NNS cord-293913-frkb8iso 92 61 ( ( -LRB- cord-293913-frkb8iso 92 62 TNT TNT NNP cord-293913-frkb8iso 92 63 system system NN cord-293913-frkb8iso 92 64 , , , cord-293913-frkb8iso 92 65 Promega Promega NNP cord-293913-frkb8iso 92 66 , , , cord-293913-frkb8iso 92 67 Madison Madison NNP cord-293913-frkb8iso 92 68 , , , cord-293913-frkb8iso 92 69 WI WI NNP cord-293913-frkb8iso 92 70 ) ) -RRB- cord-293913-frkb8iso 92 71 . . . cord-293913-frkb8iso 93 1 The the DT cord-293913-frkb8iso 93 2 translation translation NN cord-293913-frkb8iso 93 3 products product NNS cord-293913-frkb8iso 93 4 were be VBD cord-293913-frkb8iso 93 5 analyzed analyze VBN cord-293913-frkb8iso 93 6 by by IN cord-293913-frkb8iso 93 7 SDSpolyacrylamide SDSpolyacrylamide NNP cord-293913-frkb8iso 93 8 gel gel NN cord-293913-frkb8iso 93 9 etectrophoresis etectrophoresis NN cord-293913-frkb8iso 93 10 ( ( -LRB- cord-293913-frkb8iso 93 11 SDS SDS NNP cord-293913-frkb8iso 93 12 - - HYPH cord-293913-frkb8iso 93 13 PAGE PAGE NNP cord-293913-frkb8iso 93 14 ) ) -RRB- cord-293913-frkb8iso 93 15 directly directly RB cord-293913-frkb8iso 93 16 ( ( -LRB- cord-293913-frkb8iso 93 17 Fig Fig NNP cord-293913-frkb8iso 93 18 . . NNP cord-293913-frkb8iso 93 19 4 4 CD cord-293913-frkb8iso 93 20 , , , cord-293913-frkb8iso 93 21 Lanes lane NNS cord-293913-frkb8iso 93 22 1 1 CD cord-293913-frkb8iso 93 23 and and CC cord-293913-frkb8iso 93 24 2 2 CD cord-293913-frkb8iso 93 25 ) ) -RRB- cord-293913-frkb8iso 93 26 or or CC cord-293913-frkb8iso 93 27 after after IN cord-293913-frkb8iso 93 28 immunoprecipitation immunoprecipitation NN cord-293913-frkb8iso 93 29 with with IN cord-293913-frkb8iso 93 30 anti-647 anti-647 NNP cord-293913-frkb8iso 93 31 ( ( -LRB- cord-293913-frkb8iso 93 32 Lanes Lanes NNP cord-293913-frkb8iso 93 33 4 4 CD cord-293913-frkb8iso 93 34 and and CC cord-293913-frkb8iso 93 35 5 5 CD cord-293913-frkb8iso 93 36 ) ) -RRB- cord-293913-frkb8iso 93 37 or or CC cord-293913-frkb8iso 93 38 anti anti JJ cord-293913-frkb8iso 93 39 - - JJ cord-293913-frkb8iso 93 40 p28 p28 JJ cord-293913-frkb8iso 93 41 ( ( -LRB- cord-293913-frkb8iso 93 42 Lanes Lanes NNP cord-293913-frkb8iso 93 43 6 6 CD cord-293913-frkb8iso 93 44 and and CC cord-293913-frkb8iso 93 45 7 7 CD cord-293913-frkb8iso 93 46 ) ) -RRB- cord-293913-frkb8iso 93 47 . . . cord-293913-frkb8iso 94 1 As as IN cord-293913-frkb8iso 94 2 expected expect VBN cord-293913-frkb8iso 94 3 , , , cord-293913-frkb8iso 94 4 p28 p28 NNP cord-293913-frkb8iso 94 5 is be VBZ cord-293913-frkb8iso 94 6 detected detect VBN cord-293913-frkb8iso 94 7 from from IN cord-293913-frkb8iso 94 8 the the DT cord-293913-frkb8iso 94 9 primary primary JJ cord-293913-frkb8iso 94 10 translation translation NN cord-293913-frkb8iso 94 11 products product NNS cord-293913-frkb8iso 94 12 of of IN cord-293913-frkb8iso 94 13 both both DT cord-293913-frkb8iso 94 14 pT7-NA6 pt7-na6 JJ cord-293913-frkb8iso 94 15 and and CC cord-293913-frkb8iso 94 16 pT7-NBgl pt7-nbgl JJ cord-293913-frkb8iso 94 17 , , , cord-293913-frkb8iso 94 18 since since IN cord-293913-frkb8iso 94 19 both both DT cord-293913-frkb8iso 94 20 encode encode VB cord-293913-frkb8iso 94 21 PCP-1 PCP-1 NNP cord-293913-frkb8iso 94 22 , , , cord-293913-frkb8iso 94 23 and and CC cord-293913-frkb8iso 94 24 it -PRON- PRP cord-293913-frkb8iso 94 25 has have VBZ cord-293913-frkb8iso 94 26 been be VBN cord-293913-frkb8iso 94 27 shown show VBN cord-293913-frkb8iso 94 28 that that IN cord-293913-frkb8iso 94 29 PCP-1 PCP-1 NNP cord-293913-frkb8iso 94 30 is be VBZ cord-293913-frkb8iso 94 31 responsible responsible JJ cord-293913-frkb8iso 94 32 for for IN cord-293913-frkb8iso 94 33 the the DT cord-293913-frkb8iso 94 34 cleavage cleavage NN cord-293913-frkb8iso 94 35 of of IN cord-293913-frkb8iso 94 36 p28 p28 NN cord-293913-frkb8iso 94 37 ( ( -LRB- cord-293913-frkb8iso 95 1 Baker Baker NNP cord-293913-frkb8iso 95 2 et et NNP cord-293913-frkb8iso 95 3 al al NNP cord-293913-frkb8iso 95 4 . . NNP cord-293913-frkb8iso 95 5 , , , cord-293913-frkb8iso 95 6 1989 1989 CD cord-293913-frkb8iso 95 7 ) ) -RRB- cord-293913-frkb8iso 95 8 . . . cord-293913-frkb8iso 96 1 Clone Clone NNP cord-293913-frkb8iso 96 2 pT7-NA6 pT7-NA6 NNP cord-293913-frkb8iso 96 3 contains contain VBZ cord-293913-frkb8iso 96 4 an an DT cord-293913-frkb8iso 96 5 extension extension NN cord-293913-frkb8iso 96 6 of of IN cord-293913-frkb8iso 96 7 the the DT cord-293913-frkb8iso 96 8 5'-end 5'-end CD cord-293913-frkb8iso 96 9 of of IN cord-293913-frkb8iso 96 10 the the DT cord-293913-frkb8iso 96 11 polymerase polymerase NN cord-293913-frkb8iso 96 12 gene gene NN cord-293913-frkb8iso 96 13 which which WDT cord-293913-frkb8iso 96 14 includes include VBZ cord-293913-frkb8iso 96 15 the the DT cord-293913-frkb8iso 96 16 putative putative JJ cord-293913-frkb8iso 96 17 PCP-2 PCP-2 NNP cord-293913-frkb8iso 96 18 domain domain NN cord-293913-frkb8iso 96 19 . . . cord-293913-frkb8iso 97 1 Analysis analysis NN cord-293913-frkb8iso 97 2 of of IN cord-293913-frkb8iso 97 3 pT7-NA6 pT7-NA6 NNP cord-293913-frkb8iso 97 4 in in IN cord-293913-frkb8iso 97 5 vitro vitro FW cord-293913-frkb8iso 97 6 translation translation NN cord-293913-frkb8iso 97 7 products product NNS cord-293913-frkb8iso 97 8 reveals reveal VBZ cord-293913-frkb8iso 97 9 protein protein NN cord-293913-frkb8iso 97 10 products product NNS cord-293913-frkb8iso 97 11 of of IN cord-293913-frkb8iso 97 12 approximately approximately RB cord-293913-frkb8iso 97 13 195 195 CD cord-293913-frkb8iso 97 14 and and CC cord-293913-frkb8iso 97 15 28 28 CD cord-293913-frkb8iso 97 16 kDa kDa NNS cord-293913-frkb8iso 97 17 . . . cord-293913-frkb8iso 98 1 No no DT cord-293913-frkb8iso 98 2 additional additional JJ cord-293913-frkb8iso 98 3 proteolytic proteolytic JJ cord-293913-frkb8iso 98 4 products product NNS cord-293913-frkb8iso 98 5 were be VBD cord-293913-frkb8iso 98 6 specifically specifically RB cord-293913-frkb8iso 98 7 detected detect VBN cord-293913-frkb8iso 98 8 either either CC cord-293913-frkb8iso 98 9 in in IN cord-293913-frkb8iso 98 10 the the DT cord-293913-frkb8iso 98 11 primary primary JJ cord-293913-frkb8iso 98 12 translation translation NN cord-293913-frkb8iso 98 13 products product NNS cord-293913-frkb8iso 98 14 ( ( -LRB- cord-293913-frkb8iso 98 15 Fig Fig NNP cord-293913-frkb8iso 98 16 . . NNP cord-293913-frkb8iso 98 17 4 4 CD cord-293913-frkb8iso 98 18 , , , cord-293913-frkb8iso 98 19 Lane Lane NNP cord-293913-frkb8iso 98 20 1 1 CD cord-293913-frkb8iso 98 21 ) ) -RRB- cord-293913-frkb8iso 98 22 or or CC cord-293913-frkb8iso 98 23 after after IN cord-293913-frkb8iso 98 24 immunoprecipitation immunoprecipitation NN cord-293913-frkb8iso 98 25 with with IN cord-293913-frkb8iso 98 26 anti-647 anti-647 NNP cord-293913-frkb8iso 98 27 ( ( -LRB- cord-293913-frkb8iso 98 28 Lane Lane NNP cord-293913-frkb8iso 98 29 4 4 CD cord-293913-frkb8iso 98 30 ) ) -RRB- cord-293913-frkb8iso 98 31 . . . cord-293913-frkb8iso 99 1 These these DT cord-293913-frkb8iso 99 2 results result NNS cord-293913-frkb8iso 99 3 indicate indicate VBP cord-293913-frkb8iso 99 4 that that IN cord-293913-frkb8iso 99 5 although although IN cord-293913-frkb8iso 99 6 PCP-1 PCP-1 NNP cord-293913-frkb8iso 99 7 can can MD cord-293913-frkb8iso 99 8 cleave cleave VB cord-293913-frkb8iso 99 9 p28 p28 NN cord-293913-frkb8iso 99 10 from from IN cord-293913-frkb8iso 99 11 the the DT cord-293913-frkb8iso 99 12 polyprotein polyprotein NN cord-293913-frkb8iso 99 13 representing represent VBG cord-293913-frkb8iso 99 14 the the DT cord-293913-frkb8iso 99 15 5 5 CD cord-293913-frkb8iso 99 16 ' ' POS cord-293913-frkb8iso 99 17 6.8 6.8 CD cord-293913-frkb8iso 99 18 kb kb NNS cord-293913-frkb8iso 99 19 of of IN cord-293913-frkb8iso 99 20 ORF ORF NNP cord-293913-frkb8iso 99 21 - - HYPH cord-293913-frkb8iso 99 22 la la NNP cord-293913-frkb8iso 99 23 , , , cord-293913-frkb8iso 99 24 neither neither CC cord-293913-frkb8iso 99 25 PCP-1 PCP-1 NNP cord-293913-frkb8iso 99 26 nor nor CC cord-293913-frkb8iso 99 27 PCP-2 PCP-2 NNP cord-293913-frkb8iso 99 28 can can MD cord-293913-frkb8iso 99 29 cleave cleave VB cord-293913-frkb8iso 99 30 p72 p72 NN cord-293913-frkb8iso 99 31 or or CC cord-293913-frkb8iso 99 32 p65 p65 NN cord-293913-frkb8iso 99 33 from from IN cord-293913-frkb8iso 99 34 the the DT cord-293913-frkb8iso 99 35 precursor precursor NN cord-293913-frkb8iso 99 36 polyprotein polyprotein NN cord-293913-frkb8iso 99 37 in in IN cord-293913-frkb8iso 99 38 vitro vitro FW cord-293913-frkb8iso 99 39 . . . cord-293913-frkb8iso 100 1 Although although IN cord-293913-frkb8iso 100 2 the the DT cord-293913-frkb8iso 100 3 viral viral JJ cord-293913-frkb8iso 100 4 proteinase proteinase NN cord-293913-frkb8iso 100 5 domains domain NNS cord-293913-frkb8iso 100 6 PCP-1 PCP-1 NNP cord-293913-frkb8iso 100 7 and and CC cord-293913-frkb8iso 100 8 PCP-2 PCP-2 NNP cord-293913-frkb8iso 100 9 do do VBP cord-293913-frkb8iso 100 10 not not RB cord-293913-frkb8iso 100 11 cleave cleave VB cord-293913-frkb8iso 100 12 p72 p72 NN cord-293913-frkb8iso 100 13 or or CC cord-293913-frkb8iso 100 14 p65 p65 NN cord-293913-frkb8iso 100 15 from from IN cord-293913-frkb8iso 100 16 the the DT cord-293913-frkb8iso 100 17 precursor precursor NN cord-293913-frkb8iso 100 18 polyproteins polyprotein NNS cord-293913-frkb8iso 100 19 described describe VBN cord-293913-frkb8iso 100 20 above above RB cord-293913-frkb8iso 100 21 , , , cord-293913-frkb8iso 100 22 they -PRON- PRP cord-293913-frkb8iso 100 23 may may MD cord-293913-frkb8iso 100 24 still still RB cord-293913-frkb8iso 100 25 be be VB cord-293913-frkb8iso 100 26 responsible responsible JJ cord-293913-frkb8iso 100 27 for for IN cord-293913-frkb8iso 100 28 the the DT cord-293913-frkb8iso 100 29 cleavage cleavage NN cord-293913-frkb8iso 100 30 of of IN cord-293913-frkb8iso 100 31 p65 p65 NN cord-293913-frkb8iso 100 32 if if IN cord-293913-frkb8iso 100 33 the the DT cord-293913-frkb8iso 100 34 appropriate appropriate JJ cord-293913-frkb8iso 100 35 precursor precursor NN cord-293913-frkb8iso 100 36 , , , cord-293913-frkb8iso 100 37 p72 p72 NN cord-293913-frkb8iso 100 38 , , , cord-293913-frkb8iso 100 39 is be VBZ cord-293913-frkb8iso 100 40 presented present VBN cord-293913-frkb8iso 100 41 . . . cord-293913-frkb8iso 101 1 Alternatively alternatively RB cord-293913-frkb8iso 101 2 , , , cord-293913-frkb8iso 101 3 p72 p72 NN cord-293913-frkb8iso 101 4 may may MD cord-293913-frkb8iso 101 5 be be VB cord-293913-frkb8iso 101 6 processed process VBN cord-293913-frkb8iso 101 7 by by IN cord-293913-frkb8iso 101 8 MHV MHV NNP cord-293913-frkb8iso 101 9 proteinase proteinase NN cord-293913-frkb8iso 101 10 3C 3c NN cord-293913-frkb8iso 101 11 - - HYPH cord-293913-frkb8iso 101 12 pro pro JJ cord-293913-frkb8iso 101 13 or or CC cord-293913-frkb8iso 101 14 some some DT cord-293913-frkb8iso 101 15 other other JJ cord-293913-frkb8iso 101 16 unidentified unidentified JJ cord-293913-frkb8iso 101 17 viral viral JJ cord-293913-frkb8iso 101 18 proteinase proteinase NN cord-293913-frkb8iso 101 19 , , , cord-293913-frkb8iso 101 20 to to TO cord-293913-frkb8iso 101 21 generate generate VB cord-293913-frkb8iso 101 22 p65 p65 CD cord-293913-frkb8iso 101 23 . . . cord-293913-frkb8iso 102 1 To to TO cord-293913-frkb8iso 102 2 address address VB cord-293913-frkb8iso 102 3 this this DT cord-293913-frkb8iso 102 4 possibility possibility NN cord-293913-frkb8iso 102 5 we -PRON- PRP cord-293913-frkb8iso 102 6 asked ask VBD cord-293913-frkb8iso 102 7 the the DT cord-293913-frkb8iso 102 8 following following JJ cord-293913-frkb8iso 102 9 question question NN cord-293913-frkb8iso 102 10 : : : cord-293913-frkb8iso 102 11 If if IN cord-293913-frkb8iso 102 12 p72 p72 NN cord-293913-frkb8iso 102 13 is be VBZ cord-293913-frkb8iso 102 14 presented present VBN cord-293913-frkb8iso 102 15 independently independently RB cord-293913-frkb8iso 102 16 , , , cord-293913-frkb8iso 102 17 will will MD cord-293913-frkb8iso 102 18 a a DT cord-293913-frkb8iso 102 19 viral viral JJ cord-293913-frkb8iso 102 20 proteinase proteinase NN cord-293913-frkb8iso 102 21 domain domain NN cord-293913-frkb8iso 102 22 recognize recognize VBP cord-293913-frkb8iso 102 23 and and CC cord-293913-frkb8iso 102 24 cleave cleave VB cord-293913-frkb8iso 102 25 it -PRON- PRP cord-293913-frkb8iso 102 26 to to TO cord-293913-frkb8iso 102 27 produce produce VB cord-293913-frkb8iso 102 28 p65 p65 CD cord-293913-frkb8iso 102 29 ? ? . cord-293913-frkb8iso 103 1 For for IN cord-293913-frkb8iso 103 2 this this DT cord-293913-frkb8iso 103 3 purpose purpose NN cord-293913-frkb8iso 103 4 , , , cord-293913-frkb8iso 103 5 we -PRON- PRP cord-293913-frkb8iso 103 6 reverse reverse VBP cord-293913-frkb8iso 103 7 - - HYPH cord-293913-frkb8iso 103 8 transcribed transcribe VBN cord-293913-frkb8iso 103 9 and and CC cord-293913-frkb8iso 103 10 polymerase polymerase NN cord-293913-frkb8iso 103 11 chain chain NN cord-293913-frkb8iso 103 12 reaction reaction NN cord-293913-frkb8iso 103 13 ( ( -LRB- cord-293913-frkb8iso 103 14 PCR PCR NNP cord-293913-frkb8iso 103 15 ) ) -RRB- cord-293913-frkb8iso 103 16 amplified amplify VBD cord-293913-frkb8iso 103 17 the the DT cord-293913-frkb8iso 103 18 72 72 CD cord-293913-frkb8iso 103 19 kDa kDa NNS cord-293913-frkb8iso 103 20 coding code VBG cord-293913-frkb8iso 103 21 region region NN cord-293913-frkb8iso 103 22 adjacent adjacent JJ cord-293913-frkb8iso 103 23 to to IN cord-293913-frkb8iso 103 24 p28 p28 NN cord-293913-frkb8iso 103 25 starting start VBG cord-293913-frkb8iso 103 26 at at IN cord-293913-frkb8iso 103 27 nucleotide nucleotide NN cord-293913-frkb8iso 103 28 956 956 CD cord-293913-frkb8iso 103 29 , , , cord-293913-frkb8iso 103 30 encoding encode VBG cord-293913-frkb8iso 103 31 the the DT cord-293913-frkb8iso 103 32 valine valine NN cord-293913-frkb8iso 103 33 in in IN cord-293913-frkb8iso 103 34 the the DT cord-293913-frkb8iso 103 35 PI pi NN cord-293913-frkb8iso 103 36 ' ' '' cord-293913-frkb8iso 103 37 position position NN cord-293913-frkb8iso 103 38 of of IN cord-293913-frkb8iso 103 39 the the DT cord-293913-frkb8iso 103 40 p28 p28 CD cord-293913-frkb8iso 103 41 cleavage cleavage NN cord-293913-frkb8iso 103 42 site site NN cord-293913-frkb8iso 103 43 , , , cord-293913-frkb8iso 103 44 and and CC cord-293913-frkb8iso 103 45 ending end VBG cord-293913-frkb8iso 103 46 precisely precisely RB cord-293913-frkb8iso 103 47 at at IN cord-293913-frkb8iso 103 48 a a DT cord-293913-frkb8iso 103 49 potential potential JJ cord-293913-frkb8iso 103 50 3C 3c NN cord-293913-frkb8iso 103 51 - - HYPH cord-293913-frkb8iso 103 52 pro pro NNP cord-293913-frkb8iso 103 53 E E NNP cord-293913-frkb8iso 103 54 / / SYM cord-293913-frkb8iso 103 55 S s NN cord-293913-frkb8iso 103 56 cleavage cleavage NN cord-293913-frkb8iso 103 57 site site NN cord-293913-frkb8iso 103 58 , , , cord-293913-frkb8iso 103 59 at at IN cord-293913-frkb8iso 103 60 nucleotide nucleotide JJ cord-293913-frkb8iso 103 61 2896 2896 CD cord-293913-frkb8iso 103 62 . . . cord-293913-frkb8iso 104 1 This this DT cord-293913-frkb8iso 104 2 region region NN cord-293913-frkb8iso 104 3 was be VBD cord-293913-frkb8iso 104 4 cloned clone VBN cord-293913-frkb8iso 104 5 into into IN cord-293913-frkb8iso 104 6 the the DT cord-293913-frkb8iso 104 7 expression expression NN cord-293913-frkb8iso 104 8 vector vector NN cord-293913-frkb8iso 104 9 pET-1 pET-1 NNP cord-293913-frkb8iso 104 10 ld ld NNP cord-293913-frkb8iso 104 11 ( ( -LRB- cord-293913-frkb8iso 104 12 Novagen Novagen NNP cord-293913-frkb8iso 104 13 , , , cord-293913-frkb8iso 104 14 Madison Madison NNP cord-293913-frkb8iso 104 15 , , , cord-293913-frkb8iso 104 16 WI WI NNP cord-293913-frkb8iso 104 17 ) ) -RRB- cord-293913-frkb8iso 104 18 , , , cord-293913-frkb8iso 104 19 generating generate VBG cord-293913-frkb8iso 104 20 plasmid plasmid NN cord-293913-frkb8iso 105 1 pET pet NN cord-293913-frkb8iso 105 2 - - HYPH cord-293913-frkb8iso 105 3 lid lid NN cord-293913-frkb8iso 105 4 / / SYM cord-293913-frkb8iso 105 5 p72 p72 NN cord-293913-frkb8iso 105 6 ( ( -LRB- cord-293913-frkb8iso 105 7 Fig Fig NNP cord-293913-frkb8iso 105 8 . . . cord-293913-frkb8iso 105 9 1 1 CD cord-293913-frkb8iso 105 10 ) ) -RRB- cord-293913-frkb8iso 105 11 . . . cord-293913-frkb8iso 106 1 In in IN cord-293913-frkb8iso 106 2 vitro vitro FW cord-293913-frkb8iso 106 3 transcription transcription NN cord-293913-frkb8iso 106 4 / / SYM cord-293913-frkb8iso 106 5 translation translation NN cord-293913-frkb8iso 106 6 of of IN cord-293913-frkb8iso 106 7 this this DT cord-293913-frkb8iso 106 8 plasmid plasmid NN cord-293913-frkb8iso 106 9 DNA dna NN cord-293913-frkb8iso 106 10 yields yield VBZ cord-293913-frkb8iso 106 11 a a DT cord-293913-frkb8iso 106 12 protein protein NN cord-293913-frkb8iso 106 13 which which WDT cord-293913-frkb8iso 106 14 , , , cord-293913-frkb8iso 106 15 when when WRB cord-293913-frkb8iso 106 16 analyzed analyze VBN cord-293913-frkb8iso 106 17 by by IN cord-293913-frkb8iso 106 18 SDS SDS NNP cord-293913-frkb8iso 106 19 - - HYPH cord-293913-frkb8iso 106 20 PAGE PAGE NNP cord-293913-frkb8iso 106 21 , , , cord-293913-frkb8iso 106 22 is be VBZ cord-293913-frkb8iso 106 23 approximately approximately RB cord-293913-frkb8iso 106 24 72 72 CD cord-293913-frkb8iso 106 25 kDa kDa NNS cord-293913-frkb8iso 106 26 ( ( -LRB- cord-293913-frkb8iso 106 27 Fig Fig NNP cord-293913-frkb8iso 106 28 . . . cord-293913-frkb8iso 106 29 5(A 5(a CD cord-293913-frkb8iso 106 30 ) ) -RRB- cord-293913-frkb8iso 106 31 , , , cord-293913-frkb8iso 106 32 Lane Lane NNP cord-293913-frkb8iso 106 33 1 1 CD cord-293913-frkb8iso 106 34 ) ) -RRB- cord-293913-frkb8iso 106 35 . . . cord-293913-frkb8iso 107 1 To to TO cord-293913-frkb8iso 107 2 determine determine VB cord-293913-frkb8iso 107 3 if if IN cord-293913-frkb8iso 107 4 MHV MHV NNP cord-293913-frkb8iso 107 5 proteinase proteinase NN cord-293913-frkb8iso 107 6 domains domain NNS cord-293913-frkb8iso 107 7 PCP-1 PCP-1 NNP cord-293913-frkb8iso 107 8 or or CC cord-293913-frkb8iso 107 9 PCP-2 PCP-2 NNP cord-293913-frkb8iso 107 10 act act VBP cord-293913-frkb8iso 107 11 in in IN cord-293913-frkb8iso 107 12 trans trans NNP cord-293913-frkb8iso 107 13 to to TO cord-293913-frkb8iso 107 14 cleave cleave VB cord-293913-frkb8iso 107 15 p72 p72 NN cord-293913-frkb8iso 107 16 , , , cord-293913-frkb8iso 107 17 we -PRON- PRP cord-293913-frkb8iso 107 18 generated generate VBD cord-293913-frkb8iso 107 19 radiolabeled radiolabeled JJ cord-293913-frkb8iso 107 20 p72 p72 NN cord-293913-frkb8iso 107 21 protein protein NN cord-293913-frkb8iso 107 22 and and CC cord-293913-frkb8iso 107 23 unlabeled unlabeled JJ cord-293913-frkb8iso 107 24 pT7-NBgl pT7-NBgl NNS cord-293913-frkb8iso 107 25 and and CC cord-293913-frkb8iso 107 26 pT7-NA6 pT7-NA6 . cord-293913-frkb8iso 108 1 The the DT cord-293913-frkb8iso 108 2 translation translation NN cord-293913-frkb8iso 108 3 products product NNS cord-293913-frkb8iso 108 4 were be VBD cord-293913-frkb8iso 108 5 analyzed analyze VBN cord-293913-frkb8iso 108 6 by by IN cord-293913-frkb8iso 108 7 10 10 CD cord-293913-frkb8iso 108 8 % % NN cord-293913-frkb8iso 108 9 SDS SDS NNP cord-293913-frkb8iso 108 10 - - HYPH cord-293913-frkb8iso 108 11 PAGE PAGE NNP cord-293913-frkb8iso 108 12 directly directly RB cord-293913-frkb8iso 108 13 ( ( -LRB- cord-293913-frkb8iso 108 14 Lanes lane NNS cord-293913-frkb8iso 108 15 1 1 CD cord-293913-frkb8iso 108 16 and and CC cord-293913-frkb8iso 108 17 2 2 CD cord-293913-frkb8iso 108 18 ) ) -RRB- cord-293913-frkb8iso 108 19 or or CC cord-293913-frkb8iso 108 20 following follow VBG cord-293913-frkb8iso 108 21 immunoprecipitation immunoprecipitation NN cord-293913-frkb8iso 108 22 with with IN cord-293913-frkb8iso 108 23 anti-647 anti-647 NNP cord-293913-frkb8iso 109 1 ( ( -LRB- cord-293913-frkb8iso 109 2 Lanes Lanes NNP cord-293913-frkb8iso 109 3 4 4 CD cord-293913-frkb8iso 109 4 and and CC cord-293913-frkb8iso 109 5 5 5 CD cord-293913-frkb8iso 109 6 ) ) -RRB- cord-293913-frkb8iso 109 7 or or CC cord-293913-frkb8iso 109 8 anti anti JJ cord-293913-frkb8iso 109 9 - - JJ cord-293913-frkb8iso 109 10 p28 p28 JJ cord-293913-frkb8iso 109 11 ( ( -LRB- cord-293913-frkb8iso 109 12 Lanes Lanes NNP cord-293913-frkb8iso 109 13 6 6 CD cord-293913-frkb8iso 109 14 and and CC cord-293913-frkb8iso 109 15 7 7 CD cord-293913-frkb8iso 109 16 ) ) -RRB- cord-293913-frkb8iso 109 17 . . . cord-293913-frkb8iso 110 1 Dots dot NNS cord-293913-frkb8iso 110 2 highlite highlite VBP cord-293913-frkb8iso 110 3 higher high JJR cord-293913-frkb8iso 110 4 molecular molecular JJ cord-293913-frkb8iso 110 5 weight weight NN cord-293913-frkb8iso 110 6 immunoprecipitated immunoprecipitate VBN cord-293913-frkb8iso 110 7 products product NNS cord-293913-frkb8iso 110 8 . . . cord-293913-frkb8iso 111 1 Lane lane NN cord-293913-frkb8iso 111 2 3 3 CD cord-293913-frkb8iso 111 3 contains contain VBZ cord-293913-frkb8iso 111 4 t4C t4c NN cord-293913-frkb8iso 111 5 - - HYPH cord-293913-frkb8iso 111 6 labeled label VBN cord-293913-frkb8iso 111 7 molecular molecular JJ cord-293913-frkb8iso 111 8 marker marker NN cord-293913-frkb8iso 111 9 polypeptides polypeptide NNS cord-293913-frkb8iso 111 10 . . . cord-293913-frkb8iso 112 1 Molecular molecular JJ cord-293913-frkb8iso 112 2 mass mass NN cord-293913-frkb8iso 112 3 standards standard NNS cord-293913-frkb8iso 112 4 are be VBP cord-293913-frkb8iso 112 5 given give VBN cord-293913-frkb8iso 112 6 in in IN cord-293913-frkb8iso 112 7 kilodaltons kilodalton NNS cord-293913-frkb8iso 112 8 . . . cord-293913-frkb8iso 113 1 translation translation NN cord-293913-frkb8iso 113 2 products product NNS cord-293913-frkb8iso 113 3 , , , cord-293913-frkb8iso 113 4 mixed mix VBD cord-293913-frkb8iso 113 5 the the DT cord-293913-frkb8iso 113 6 proteins protein NNS cord-293913-frkb8iso 113 7 and and CC cord-293913-frkb8iso 113 8 incubated incubate VBD cord-293913-frkb8iso 113 9 the the DT cord-293913-frkb8iso 113 10 mixtures mixture NNS cord-293913-frkb8iso 113 11 for for IN cord-293913-frkb8iso 113 12 60 60 CD cord-293913-frkb8iso 113 13 or or CC cord-293913-frkb8iso 113 14 120 120 CD cord-293913-frkb8iso 113 15 min min NN cord-293913-frkb8iso 113 16 . . . cord-293913-frkb8iso 114 1 The the DT cord-293913-frkb8iso 114 2 proteins protein NNS cord-293913-frkb8iso 114 3 were be VBD cord-293913-frkb8iso 114 4 then then RB cord-293913-frkb8iso 114 5 analyzed analyze VBN cord-293913-frkb8iso 114 6 by by IN cord-293913-frkb8iso 114 7 electrophoresis electrophoresis NN cord-293913-frkb8iso 114 8 on on IN cord-293913-frkb8iso 114 9 an an DT cord-293913-frkb8iso 114 10 SDS sds NN cord-293913-frkb8iso 114 11 - - HYPH cord-293913-frkb8iso 114 12 polyacrylamide polyacrylamide NN cord-293913-frkb8iso 114 13 gel gel NN cord-293913-frkb8iso 114 14 and and CC cord-293913-frkb8iso 114 15 visualized visualize VBN cord-293913-frkb8iso 114 16 by by IN cord-293913-frkb8iso 114 17 autoradiography autoradiography NNP cord-293913-frkb8iso 114 18 ( ( -LRB- cord-293913-frkb8iso 114 19 Fig Fig NNP cord-293913-frkb8iso 114 20 . . NNP cord-293913-frkb8iso 114 21 5 5 CD cord-293913-frkb8iso 114 22 ) ) -RRB- cord-293913-frkb8iso 114 23 . . . cord-293913-frkb8iso 115 1 In in IN cord-293913-frkb8iso 115 2 samples sample NNS cord-293913-frkb8iso 115 3 where where WRB cord-293913-frkb8iso 115 4 fullytranslated fullytranslated JJ cord-293913-frkb8iso 115 5 proteins protein NNS cord-293913-frkb8iso 115 6 are be VBP cord-293913-frkb8iso 115 7 mixed mixed JJ cord-293913-frkb8iso 115 8 and and CC cord-293913-frkb8iso 115 9 incubated incubate VBN cord-293913-frkb8iso 115 10 together together RB cord-293913-frkb8iso 115 11 for for IN cord-293913-frkb8iso 115 12 an an DT cord-293913-frkb8iso 115 13 additional additional JJ cord-293913-frkb8iso 115 14 60 60 CD cord-293913-frkb8iso 115 15 min min NN cord-293913-frkb8iso 115 16 , , , cord-293913-frkb8iso 115 17 p72 p72 NN cord-293913-frkb8iso 115 18 is be VBZ cord-293913-frkb8iso 115 19 not not RB cord-293913-frkb8iso 115 20 cleaved cleave VBN cord-293913-frkb8iso 115 21 into into IN cord-293913-frkb8iso 115 22 p65 p65 NN cord-293913-frkb8iso 115 23 by by IN cord-293913-frkb8iso 115 24 PCP-1 PCP-1 NNP cord-293913-frkb8iso 115 25 and and CC cord-293913-frkb8iso 115 26 PCP-2-containing pcp-2-containing NN cord-293913-frkb8iso 115 27 proteins protein NNS cord-293913-frkb8iso 115 28 ( ( -LRB- cord-293913-frkb8iso 115 29 translated translate VBN cord-293913-frkb8iso 115 30 from from IN cord-293913-frkb8iso 115 31 pT7-NBgl pt7-nbgl CD cord-293913-frkb8iso 115 32 and and CC cord-293913-frkb8iso 115 33 pT7-NA6 pT7-NA6 NNS cord-293913-frkb8iso 115 34 , , , cord-293913-frkb8iso 115 35 respectively respectively RB cord-293913-frkb8iso 115 36 ) ) -RRB- cord-293913-frkb8iso 116 1 ( ( -LRB- cord-293913-frkb8iso 116 2 Fig Fig NNP cord-293913-frkb8iso 116 3 . . . cord-293913-frkb8iso 116 4 5(A 5(a CD cord-293913-frkb8iso 116 5 ) ) -RRB- cord-293913-frkb8iso 116 6 , , , cord-293913-frkb8iso 116 7 Lanes Lanes NNP cord-293913-frkb8iso 116 8 3 3 CD cord-293913-frkb8iso 116 9 - - SYM cord-293913-frkb8iso 116 10 6 6 CD cord-293913-frkb8iso 116 11 ) ) -RRB- cord-293913-frkb8iso 116 12 . . . cord-293913-frkb8iso 117 1 Similarly similarly RB cord-293913-frkb8iso 117 2 , , , cord-293913-frkb8iso 117 3 when when WRB cord-293913-frkb8iso 117 4 p72 p72 NN cord-293913-frkb8iso 117 5 is be VBZ cord-293913-frkb8iso 117 6 included include VBN cord-293913-frkb8iso 117 7 in in IN cord-293913-frkb8iso 117 8 the the DT cord-293913-frkb8iso 117 9 pT7-NBgl pt7-nbgl JJ cord-293913-frkb8iso 117 10 and and CC cord-293913-frkb8iso 117 11 pT7-NA6 pt7-na6 JJ cord-293913-frkb8iso 117 12 translation translation NN cord-293913-frkb8iso 117 13 mixtures mixture NNS cord-293913-frkb8iso 117 14 to to TO cord-293913-frkb8iso 117 15 test test VB cord-293913-frkb8iso 117 16 the the DT cord-293913-frkb8iso 117 17 possibility possibility NN cord-293913-frkb8iso 117 18 of of IN cord-293913-frkb8iso 117 19 a a DT cord-293913-frkb8iso 117 20 co co JJ cord-293913-frkb8iso 117 21 - - JJ cord-293913-frkb8iso 117 22 translational translational JJ cord-293913-frkb8iso 117 23 activity activity NN cord-293913-frkb8iso 117 24 of of IN cord-293913-frkb8iso 118 1 PCP-1 PCP-1 NNP cord-293913-frkb8iso 118 2 and and CC cord-293913-frkb8iso 118 3 PCP-2 PCP-2 NNP cord-293913-frkb8iso 118 4 ( ( -LRB- cord-293913-frkb8iso 118 5 Fig Fig NNP cord-293913-frkb8iso 118 6 . . . cord-293913-frkb8iso 118 7 5(A 5(a CD cord-293913-frkb8iso 118 8 ) ) -RRB- cord-293913-frkb8iso 118 9 , , , cord-293913-frkb8iso 118 10 Lanes Lanes NNP cord-293913-frkb8iso 118 11 7 7 CD cord-293913-frkb8iso 118 12 - - SYM cord-293913-frkb8iso 118 13 12 12 CD cord-293913-frkb8iso 118 14 ) ) -RRB- cord-293913-frkb8iso 118 15 , , , cord-293913-frkb8iso 118 16 p72 p72 NN cord-293913-frkb8iso 118 17 also also RB cord-293913-frkb8iso 118 18 remains remain VBZ cord-293913-frkb8iso 118 19 uncleaved uncleaved JJ cord-293913-frkb8iso 118 20 . . . cord-293913-frkb8iso 119 1 Incubation incubation NN cord-293913-frkb8iso 119 2 of of IN cord-293913-frkb8iso 119 3 the the DT cord-293913-frkb8iso 119 4 p72 p72 NN cord-293913-frkb8iso 119 5 protein protein NN cord-293913-frkb8iso 119 6 with with IN cord-293913-frkb8iso 119 7 rabbit rabbit NN cord-293913-frkb8iso 119 8 reticulocyte reticulocyte NNP cord-293913-frkb8iso 119 9 lysates lysates NNP cord-293913-frkb8iso 119 10 ( ( -LRB- cord-293913-frkb8iso 119 11 Fig Fig NNP cord-293913-frkb8iso 119 12 . . . cord-293913-frkb8iso 119 13 5(A 5(a CD cord-293913-frkb8iso 119 14 ) ) -RRB- cord-293913-frkb8iso 119 15 , , , cord-293913-frkb8iso 119 16 Lanes lane NNS cord-293913-frkb8iso 119 17 1 1 CD cord-293913-frkb8iso 119 18 and and CC cord-293913-frkb8iso 119 19 2 2 CD cord-293913-frkb8iso 119 20 ) ) -RRB- cord-293913-frkb8iso 119 21 , , , cord-293913-frkb8iso 119 22 a a DT cord-293913-frkb8iso 119 23 major major JJ cord-293913-frkb8iso 119 24 component component NN cord-293913-frkb8iso 119 25 in in IN cord-293913-frkb8iso 119 26 the the DT cord-293913-frkb8iso 119 27 transcription transcription NN cord-293913-frkb8iso 119 28 / / SYM cord-293913-frkb8iso 119 29 translation translation NN cord-293913-frkb8iso 119 30 reactions reaction NNS cord-293913-frkb8iso 119 31 , , , cord-293913-frkb8iso 119 32 serves serve VBZ cord-293913-frkb8iso 119 33 as as IN cord-293913-frkb8iso 119 34 a a DT cord-293913-frkb8iso 119 35 negative negative JJ cord-293913-frkb8iso 119 36 control control NN cord-293913-frkb8iso 119 37 and and CC cord-293913-frkb8iso 119 38 reflects reflect VBZ cord-293913-frkb8iso 119 39 p72 p72 NN cord-293913-frkb8iso 119 40 stability stability NN cord-293913-frkb8iso 119 41 throughout throughout IN cord-293913-frkb8iso 119 42 the the DT cord-293913-frkb8iso 119 43 incubation incubation NN cord-293913-frkb8iso 119 44 period period NN cord-293913-frkb8iso 119 45 . . . cord-293913-frkb8iso 120 1 Results result NNS cord-293913-frkb8iso 120 2 from from IN cord-293913-frkb8iso 120 3 this this DT cord-293913-frkb8iso 120 4 experiment experiment NN cord-293913-frkb8iso 120 5 ' ' `` cord-293913-frkb8iso 120 6 indicate indicate VBP cord-293913-frkb8iso 120 7 that that IN cord-293913-frkb8iso 120 8 neither neither CC cord-293913-frkb8iso 120 9 PCP-1 PCP-1 NNP cord-293913-frkb8iso 120 10 nor nor CC cord-293913-frkb8iso 120 11 PCP-2 PCP-2 NNP cord-293913-frkb8iso 120 12 can can MD cord-293913-frkb8iso 120 13 mediate mediate VB cord-293913-frkb8iso 120 14 the the DT cord-293913-frkb8iso 120 15 trans trans NN cord-293913-frkb8iso 120 16 - - NN cord-293913-frkb8iso 120 17 cleavage cleavage NN cord-293913-frkb8iso 120 18 of of IN cord-293913-frkb8iso 120 19 p72 p72 NN cord-293913-frkb8iso 120 20 as as IN cord-293913-frkb8iso 120 21 a a DT cord-293913-frkb8iso 120 22 substrate substrate NN cord-293913-frkb8iso 120 23 to to TO cord-293913-frkb8iso 120 24 generate generate VB cord-293913-frkb8iso 120 25 p65 p65 NN cord-293913-frkb8iso 120 26 in in IN cord-293913-frkb8iso 120 27 vitro vitro FW cord-293913-frkb8iso 120 28 . . . cord-293913-frkb8iso 121 1 In in IN cord-293913-frkb8iso 121 2 summary summary NN cord-293913-frkb8iso 121 3 , , , cord-293913-frkb8iso 121 4 we -PRON- PRP cord-293913-frkb8iso 121 5 have have VBP cord-293913-frkb8iso 121 6 identified identify VBN cord-293913-frkb8iso 121 7 two two CD cord-293913-frkb8iso 121 8 proteolytic proteolytic JJ cord-293913-frkb8iso 121 9 products product NNS cord-293913-frkb8iso 121 10 , , , cord-293913-frkb8iso 121 11 p65 p65 NN cord-293913-frkb8iso 121 12 and and CC cord-293913-frkb8iso 121 13 p72 p72 NN cord-293913-frkb8iso 121 14 of of IN cord-293913-frkb8iso 121 15 the the DT cord-293913-frkb8iso 121 16 MHV MHV NNP cord-293913-frkb8iso 121 17 - - HYPH cord-293913-frkb8iso 121 18 JHM JHM NNP cord-293913-frkb8iso 121 19 polymerase polymerase NN cord-293913-frkb8iso 121 20 polyprotein polyprotein NN cord-293913-frkb8iso 121 21 , , , cord-293913-frkb8iso 121 22 from from IN cord-293913-frkb8iso 121 23 MHV MHV NNP cord-293913-frkb8iso 121 24 - - HYPH cord-293913-frkb8iso 121 25 JHM JHM NNP cord-293913-frkb8iso 121 26 infected infected JJ cord-293913-frkb8iso 121 27 cells cell NNS cord-293913-frkb8iso 121 28 . . . cord-293913-frkb8iso 122 1 A a DT cord-293913-frkb8iso 122 2 65 65 CD cord-293913-frkb8iso 122 3 kDa kDa NNP cord-293913-frkb8iso 122 4 protein protein NN cord-293913-frkb8iso 122 5 has have VBZ cord-293913-frkb8iso 122 6 also also RB cord-293913-frkb8iso 122 7 been be VBN cord-293913-frkb8iso 122 8 identified identify VBN cord-293913-frkb8iso 122 9 for for IN cord-293913-frkb8iso 122 10 MHV MHV NNP cord-293913-frkb8iso 122 11 - - HYPH cord-293913-frkb8iso 122 12 A59 A59 NNP cord-293913-frkb8iso 122 13 by by IN cord-293913-frkb8iso 122 14 Denison Denison NNP cord-293913-frkb8iso 122 15 et et NNP cord-293913-frkb8iso 122 16 al al NNP cord-293913-frkb8iso 122 17 . . . cord-293913-frkb8iso 123 1 ( ( -LRB- cord-293913-frkb8iso 123 2 1995 1995 CD cord-293913-frkb8iso 123 3 ) ) -RRB- cord-293913-frkb8iso 123 4 , , , cord-293913-frkb8iso 123 5 but but CC cord-293913-frkb8iso 123 6 the the DT cord-293913-frkb8iso 123 7 p72 p72 NN cord-293913-frkb8iso 123 8 protein protein NN cord-293913-frkb8iso 123 9 has have VBZ cord-293913-frkb8iso 123 10 not not RB cord-293913-frkb8iso 123 11 previously previously RB cord-293913-frkb8iso 123 12 been be VBN cord-293913-frkb8iso 123 13 reported report VBN cord-293913-frkb8iso 123 14 for for IN cord-293913-frkb8iso 123 15 any any DT cord-293913-frkb8iso 123 16 coronavirus coronavirus NN cord-293913-frkb8iso 123 17 . . . cord-293913-frkb8iso 124 1 Our -PRON- PRP$ cord-293913-frkb8iso 124 2 pulse pulse NN cord-293913-frkb8iso 124 3 - - HYPH cord-293913-frkb8iso 124 4 chase chase NN cord-293913-frkb8iso 124 5 experiments experiment NNS cord-293913-frkb8iso 124 6 indicate indicate VBP cord-293913-frkb8iso 124 7 that that IN cord-293913-frkb8iso 124 8 the the DT cord-293913-frkb8iso 124 9 72 72 CD cord-293913-frkb8iso 124 10 kDa kDa NNP cord-293913-frkb8iso 124 11 protein protein NN cord-293913-frkb8iso 124 12 is be VBZ cord-293913-frkb8iso 124 13 likely likely RB cord-293913-frkb8iso 124 14 a a DT cord-293913-frkb8iso 124 15 precursor precursor NN cord-293913-frkb8iso 124 16 to to IN cord-293913-frkb8iso 124 17 the the DT cord-293913-frkb8iso 124 18 p65 p65 NN cord-293913-frkb8iso 124 19 protein protein NN cord-293913-frkb8iso 124 20 ( ( -LRB- cord-293913-frkb8iso 124 21 Fig Fig NNP cord-293913-frkb8iso 124 22 . . . cord-293913-frkb8iso 124 23 3 3 CD cord-293913-frkb8iso 124 24 ) ) -RRB- cord-293913-frkb8iso 124 25 . . . cord-293913-frkb8iso 125 1 Using use VBG cord-293913-frkb8iso 125 2 both both DT cord-293913-frkb8iso 125 3 cis cis NNP cord-293913-frkb8iso 125 4 and and CC cord-293913-frkb8iso 125 5 trans trans JJ cord-293913-frkb8iso 125 6 - - JJ cord-293913-frkb8iso 125 7 cleavage cleavage JJ cord-293913-frkb8iso 125 8 assays assay NNS cord-293913-frkb8iso 125 9 , , , cord-293913-frkb8iso 125 10 we -PRON- PRP cord-293913-frkb8iso 125 11 showed show VBD cord-293913-frkb8iso 125 12 that that IN cord-293913-frkb8iso 125 13 neither neither CC cord-293913-frkb8iso 125 14 the the DT cord-293913-frkb8iso 125 15 PCP-1 PCP-1 NNP cord-293913-frkb8iso 125 16 nor nor CC cord-293913-frkb8iso 125 17 PCP-2 PCP-2 NNP cord-293913-frkb8iso 125 18 domain domain NN cord-293913-frkb8iso 125 19 mediates mediate VBZ cord-293913-frkb8iso 125 20 the the DT cord-293913-frkb8iso 125 21 cleavage cleavage NN cord-293913-frkb8iso 125 22 of of IN cord-293913-frkb8iso 125 23 p72 p72 NN cord-293913-frkb8iso 125 24 or or CC cord-293913-frkb8iso 125 25 p65 p65 NN cord-293913-frkb8iso 125 26 in in IN cord-293913-frkb8iso 125 27 vitro vitro FW cord-293913-frkb8iso 125 28 ( ( -LRB- cord-293913-frkb8iso 125 29 Figs fig NNS cord-293913-frkb8iso 125 30 . . . cord-293913-frkb8iso 126 1 4 4 CD cord-293913-frkb8iso 126 2 and and CC cord-293913-frkb8iso 126 3 5 5 CD cord-293913-frkb8iso 126 4 ) ) -RRB- cord-293913-frkb8iso 126 5 . . . cord-293913-frkb8iso 127 1 These these DT cord-293913-frkb8iso 127 2 results result NNS cord-293913-frkb8iso 127 3 suggest suggest VBP cord-293913-frkb8iso 127 4 that that IN cord-293913-frkb8iso 127 5 a a DT cord-293913-frkb8iso 127 6 proteolytic proteolytic JJ cord-293913-frkb8iso 127 7 pathway pathway NN cord-293913-frkb8iso 127 8 distinct distinct JJ cord-293913-frkb8iso 127 9 from from IN cord-293913-frkb8iso 127 10 that that DT cord-293913-frkb8iso 127 11 used use VBN cord-293913-frkb8iso 127 12 to to TO cord-293913-frkb8iso 127 13 generate generate VB cord-293913-frkb8iso 127 14 p28 p28 NN cord-293913-frkb8iso 127 15 is be VBZ cord-293913-frkb8iso 127 16 used use VBN cord-293913-frkb8iso 127 17 to to TO cord-293913-frkb8iso 127 18 generate generate VB cord-293913-frkb8iso 127 19 p72 p72 NN cord-293913-frkb8iso 127 20 and and CC cord-293913-frkb8iso 127 21 p65 p65 NN cord-293913-frkb8iso 127 22 in in IN cord-293913-frkb8iso 127 23 MHV MHV NNP cord-293913-frkb8iso 127 24 - - HYPH cord-293913-frkb8iso 127 25 JHM JHM NNP cord-293913-frkb8iso 127 26 infected infected JJ cord-293913-frkb8iso 127 27 cells cell NNS cord-293913-frkb8iso 127 28 . . . cord-293913-frkb8iso 128 1 It -PRON- PRP cord-293913-frkb8iso 128 2 is be VBZ cord-293913-frkb8iso 128 3 likely likely JJ cord-293913-frkb8iso 128 4 that that IN cord-293913-frkb8iso 128 5 a a DT cord-293913-frkb8iso 128 6 number number NN cord-293913-frkb8iso 128 7 of of IN cord-293913-frkb8iso 128 8 distinct distinct JJ cord-293913-frkb8iso 128 9 proteolytic proteolytic JJ cord-293913-frkb8iso 128 10 events event NNS cord-293913-frkb8iso 128 11 are be VBP cord-293913-frkb8iso 128 12 required require VBN cord-293913-frkb8iso 128 13 to to TO cord-293913-frkb8iso 128 14 generate generate VB cord-293913-frkb8iso 128 15 the the DT cord-293913-frkb8iso 128 16 functional functional JJ cord-293913-frkb8iso 128 17 MHV MHV NNP cord-293913-frkb8iso 128 18 RNA RNA NNP cord-293913-frkb8iso 128 19 polymerase polymerase NN cord-293913-frkb8iso 128 20 . . . cord-293913-frkb8iso 129 1 To to IN cord-293913-frkb8iso 129 2 date date NN cord-293913-frkb8iso 129 3 , , , cord-293913-frkb8iso 129 4 a a DT cord-293913-frkb8iso 129 5 number number NN cord-293913-frkb8iso 129 6 of of IN cord-293913-frkb8iso 129 7 proteins protein NNS cord-293913-frkb8iso 129 8 have have VBP cord-293913-frkb8iso 129 9 been be VBN cord-293913-frkb8iso 129 10 identified identify VBN cord-293913-frkb8iso 129 11 from from IN cord-293913-frkb8iso 129 12 MHV MHV NNP cord-293913-frkb8iso 129 13 infected infected JJ cord-293913-frkb8iso 129 14 cells cell NNS cord-293913-frkb8iso 129 15 , , , cord-293913-frkb8iso 129 16 including include VBG cord-293913-frkb8iso 129 17 p28 p28 CD cord-293913-frkb8iso 129 18 , , , cord-293913-frkb8iso 129 19 p72 p72 NN cord-293913-frkb8iso 129 20 , , , cord-293913-frkb8iso 129 21 p65 p65 NN cord-293913-frkb8iso 129 22 , , , cord-293913-frkb8iso 129 23 p50 p50 NN cord-293913-frkb8iso 129 24 , , , cord-293913-frkb8iso 129 25 p240 p240 NNP cord-293913-frkb8iso 129 26 and and CC cord-293913-frkb8iso 129 27 p290 p290 NNP cord-293913-frkb8iso 129 28 ( ( -LRB- cord-293913-frkb8iso 129 29 Denison Denison NNP cord-293913-frkb8iso 129 30 and and CC cord-293913-frkb8iso 129 31 Perlman Perlman NNP cord-293913-frkb8iso 129 32 , , , cord-293913-frkb8iso 129 33 1987 1987 CD cord-293913-frkb8iso 129 34 ; ; : cord-293913-frkb8iso 129 35 Denison Denison NNP cord-293913-frkb8iso 129 36 et et NNP cord-293913-frkb8iso 129 37 al al NNP cord-293913-frkb8iso 129 38 . . NNP cord-293913-frkb8iso 129 39 , , , cord-293913-frkb8iso 129 40 1992 1992 CD cord-293913-frkb8iso 130 1 Denison Denison NNP cord-293913-frkb8iso 130 2 et et NNP cord-293913-frkb8iso 130 3 al al NNP cord-293913-frkb8iso 130 4 . . . cord-293913-frkb8iso 131 1 , , , cord-293913-frkb8iso 131 2 , , , cord-293913-frkb8iso 131 3 1995 1995 CD cord-293913-frkb8iso 131 4 _SP cord-293913-frkb8iso 131 5 this this DT cord-293913-frkb8iso 131 6 report report NN cord-293913-frkb8iso 131 7 ) ) -RRB- cord-293913-frkb8iso 131 8 , , , cord-293913-frkb8iso 131 9 but but CC cord-293913-frkb8iso 131 10 only only RB cord-293913-frkb8iso 131 11 the the DT cord-293913-frkb8iso 131 12 proteolytic proteolytic JJ cord-293913-frkb8iso 131 13 pathway pathway NN cord-293913-frkb8iso 131 14 for for IN cord-293913-frkb8iso 131 15 generating generate VBG cord-293913-frkb8iso 131 16 p28 p28 CD cord-293913-frkb8iso 131 17 has have VBZ cord-293913-frkb8iso 131 18 been be VBN cord-293913-frkb8iso 131 19 elucidated elucidate VBN cord-293913-frkb8iso 131 20 ( ( -LRB- cord-293913-frkb8iso 132 1 Baker Baker NNP cord-293913-frkb8iso 132 2 et et NNP cord-293913-frkb8iso 132 3 al al NNP cord-293913-frkb8iso 132 4 . . . cord-293913-frkb8iso 133 1 , , , cord-293913-frkb8iso 133 2 1993 1993 CD cord-293913-frkb8iso 133 3 ; ; : cord-293913-frkb8iso 133 4 Dong Dong NNP cord-293913-frkb8iso 133 5 and and CC cord-293913-frkb8iso 133 6 Baker Baker NNP cord-293913-frkb8iso 133 7 , , , cord-293913-frkb8iso 133 8 1994 1994 CD cord-293913-frkb8iso 133 9 ; ; : cord-293913-frkb8iso 133 10 Hughes Hughes NNP cord-293913-frkb8iso 133 11 et et NNP cord-293913-frkb8iso 133 12 al al NNP cord-293913-frkb8iso 133 13 . . NNP cord-293913-frkb8iso 133 14 , , , cord-293913-frkb8iso 133 15 1995 1995 CD cord-293913-frkb8iso 133 16 ) ) -RRB- cord-293913-frkb8iso 133 17 . . . cord-293913-frkb8iso 134 1 There there EX cord-293913-frkb8iso 134 2 has have VBZ cord-293913-frkb8iso 134 3 been be VBN cord-293913-frkb8iso 134 4 no no DT cord-293913-frkb8iso 134 5 report report NN cord-293913-frkb8iso 134 6 of of IN cord-293913-frkb8iso 134 7 the the DT cord-293913-frkb8iso 134 8 cleavage cleavage NN cord-293913-frkb8iso 134 9 activity activity NN cord-293913-frkb8iso 134 10 of of IN cord-293913-frkb8iso 134 11 the the DT cord-293913-frkb8iso 134 12 PCP-2 PCP-2 NNP cord-293913-frkb8iso 134 13 domain domain NN cord-293913-frkb8iso 134 14 . . . cord-293913-frkb8iso 135 1 Re- re- XX cord-293913-frkb8iso 135 2 methionine methionine NN cord-293913-frkb8iso 135 3 for for IN cord-293913-frkb8iso 135 4 120 120 CD cord-293913-frkb8iso 135 5 min min NN cord-293913-frkb8iso 135 6 . . . cord-293913-frkb8iso 136 1 The the DT cord-293913-frkb8iso 136 2 p72 p72 NN cord-293913-frkb8iso 136 3 translation translation NN cord-293913-frkb8iso 136 4 product product NN cord-293913-frkb8iso 136 5 was be VBD cord-293913-frkb8iso 136 6 then then RB cord-293913-frkb8iso 136 7 incubated incubate VBN cord-293913-frkb8iso 136 8 with with IN cord-293913-frkb8iso 136 9 unlabeled unlabeled JJ cord-293913-frkb8iso 136 10 transcription transcription NN cord-293913-frkb8iso 136 11 / / SYM cord-293913-frkb8iso 136 12 translation translation NN cord-293913-frkb8iso 136 13 products product NNS cord-293913-frkb8iso 136 14 generated generate VBN cord-293913-frkb8iso 136 15 from from IN cord-293913-frkb8iso 136 16 : : : cord-293913-frkb8iso 136 17 rabbit rabbit NNP cord-293913-frkb8iso 136 18 reticulocyte reticulocyte NNP cord-293913-frkb8iso 136 19 lysates lysates NNP cord-293913-frkb8iso 136 20 alone alone RB cord-293913-frkb8iso 136 21 ( ( -LRB- cord-293913-frkb8iso 136 22 Lanes Lanes NNP cord-293913-frkb8iso 136 23 1 1 CD cord-293913-frkb8iso 136 24 and and CC cord-293913-frkb8iso 136 25 2 2 CD cord-293913-frkb8iso 136 26 ) ) -RRB- cord-293913-frkb8iso 136 27 , , , cord-293913-frkb8iso 136 28 pT7-NBgl pt7-nbgl JJ cord-293913-frkb8iso 136 29 plasmid plasmid NN cord-293913-frkb8iso 136 30 DNA DNA NNP cord-293913-frkb8iso 136 31 ( ( -LRB- cord-293913-frkb8iso 136 32 Lanes Lanes NNP cord-293913-frkb8iso 136 33 3 3 CD cord-293913-frkb8iso 136 34 and and CC cord-293913-frkb8iso 136 35 4 4 CD cord-293913-frkb8iso 136 36 ) ) -RRB- cord-293913-frkb8iso 136 37 or or CC cord-293913-frkb8iso 136 38 pT7-NA6 pt7-na6 JJ cord-293913-frkb8iso 136 39 plasmid plasmid NN cord-293913-frkb8iso 136 40 DNA DNA NNP cord-293913-frkb8iso 136 41 ( ( -LRB- cord-293913-frkb8iso 136 42 Lanes Lanes NNP cord-293913-frkb8iso 136 43 5 5 CD cord-293913-frkb8iso 136 44 and and CC cord-293913-frkb8iso 136 45 6 6 CD cord-293913-frkb8iso 136 46 ) ) -RRB- cord-293913-frkb8iso 136 47 . . . cord-293913-frkb8iso 137 1 To to TO cord-293913-frkb8iso 137 2 test test VB cord-293913-frkb8iso 137 3 the the DT cord-293913-frkb8iso 137 4 potential potential JJ cord-293913-frkb8iso 137 5 co co JJ cord-293913-frkb8iso 137 6 - - JJ cord-293913-frkb8iso 137 7 translational translational JJ cord-293913-frkb8iso 137 8 activity activity NN cord-293913-frkb8iso 137 9 of of IN cord-293913-frkb8iso 137 10 pT7-NBgl pt7-nbgl NN cord-293913-frkb8iso 137 11 and and CC cord-293913-frkb8iso 137 12 pT7-NA6 pt7-na6 JJ cord-293913-frkb8iso 137 13 translation translation NN cord-293913-frkb8iso 137 14 products product NNS cord-293913-frkb8iso 137 15 to to TO cord-293913-frkb8iso 137 16 cleave cleave VB cord-293913-frkb8iso 137 17 p72 p72 NN cord-293913-frkb8iso 137 18 in in IN cord-293913-frkb8iso 137 19 trans trans NNP cord-293913-frkb8iso 137 20 , , , cord-293913-frkb8iso 137 21 plasmid plasmid NNP cord-293913-frkb8iso 137 22 DNAs dna NNS cord-293913-frkb8iso 137 23 were be VBD cord-293913-frkb8iso 137 24 transcribed transcribe VBN cord-293913-frkb8iso 137 25 and and CC cord-293913-frkb8iso 137 26 translated translate VBN cord-293913-frkb8iso 137 27 in in IN cord-293913-frkb8iso 137 28 the the DT cord-293913-frkb8iso 137 29 absence absence NN cord-293913-frkb8iso 137 30 of of IN cord-293913-frkb8iso 137 31 [ [ -LRB- cord-293913-frkb8iso 137 32 35S]methionine 35s]methionine CD cord-293913-frkb8iso 137 33 , , , cord-293913-frkb8iso 137 34 but but CC cord-293913-frkb8iso 137 35 in in IN cord-293913-frkb8iso 137 36 the the DT cord-293913-frkb8iso 137 37 presence presence NN cord-293913-frkb8iso 137 38 of of IN cord-293913-frkb8iso 137 39 [ [ -LRB- cord-293913-frkb8iso 137 40 35S]methionine 35s]methionine CD cord-293913-frkb8iso 137 41 radiolabeled radiolabele VBN cord-293913-frkb8iso 137 42 p72 p72 NN cord-293913-frkb8iso 137 43 protein protein NN cord-293913-frkb8iso 137 44 ( ( -LRB- cord-293913-frkb8iso 137 45 Lanes Lanes NNP cord-293913-frkb8iso 137 46 7 7 CD cord-293913-frkb8iso 137 47 - - SYM cord-293913-frkb8iso 137 48 12 12 CD cord-293913-frkb8iso 137 49 ) ) -RRB- cord-293913-frkb8iso 137 50 . . . cord-293913-frkb8iso 138 1 The the DT cord-293913-frkb8iso 138 2 trans trans JJ cord-293913-frkb8iso 138 3 - - JJ cord-293913-frkb8iso 138 4 cleavage cleavage JJ cord-293913-frkb8iso 138 5 assay assay NN cord-293913-frkb8iso 138 6 products product NNS cord-293913-frkb8iso 138 7 were be VBD cord-293913-frkb8iso 138 8 analyzed analyze VBN cord-293913-frkb8iso 138 9 by by IN cord-293913-frkb8iso 138 10 electrophoresis electrophoresis NN cord-293913-frkb8iso 138 11 on on IN cord-293913-frkb8iso 138 12 a a DT cord-293913-frkb8iso 138 13 10 10 CD cord-293913-frkb8iso 138 14 % % NN cord-293913-frkb8iso 138 15 SDS sds NN cord-293913-frkb8iso 138 16 polyacrylamide polyacrylamide NN cord-293913-frkb8iso 138 17 gel gel NN cord-293913-frkb8iso 138 18 and and CC cord-293913-frkb8iso 138 19 visualized visualize VBN cord-293913-frkb8iso 138 20 by by IN cord-293913-frkb8iso 138 21 autoradiography autoradiography NNP cord-293913-frkb8iso 138 22 . . . cord-293913-frkb8iso 139 1 Molecular molecular JJ cord-293913-frkb8iso 139 2 weight weight NN cord-293913-frkb8iso 139 3 markers marker NNS cord-293913-frkb8iso 139 4 are be VBP cord-293913-frkb8iso 139 5 indicated indicate VBN cord-293913-frkb8iso 139 6 in in IN cord-293913-frkb8iso 139 7 kilodaltons kilodalton NNS cord-293913-frkb8iso 139 8 on on IN cord-293913-frkb8iso 139 9 the the DT cord-293913-frkb8iso 139 10 left left NN cord-293913-frkb8iso 139 11 of of IN cord-293913-frkb8iso 139 12 the the DT cord-293913-frkb8iso 139 13 gel gel NN cord-293913-frkb8iso 139 14 . . . cord-293913-frkb8iso 140 1 ( ( -LRB- cord-293913-frkb8iso 140 2 B b NN cord-293913-frkb8iso 140 3 ) ) -RRB- cord-293913-frkb8iso 141 1 In in IN cord-293913-frkb8iso 141 2 vitro vitro FW cord-293913-frkb8iso 141 3 transcription transcription NN cord-293913-frkb8iso 141 4 / / SYM cord-293913-frkb8iso 141 5 translation translation NN cord-293913-frkb8iso 141 6 products product NNS cord-293913-frkb8iso 141 7 generated generate VBN cord-293913-frkb8iso 141 8 from from IN cord-293913-frkb8iso 141 9 plasmid plasmid NN cord-293913-frkb8iso 141 10 DNA dna NN cord-293913-frkb8iso 141 11 pT7-NA6 pT7-NA6 NNP cord-293913-frkb8iso 141 12 ( ( -LRB- cord-293913-frkb8iso 141 13 Lane lane NN cord-293913-frkb8iso 141 14 1 1 CD cord-293913-frkb8iso 141 15 ) ) -RRB- cord-293913-frkb8iso 141 16 and and CC cord-293913-frkb8iso 141 17 pT7-NBgl pt7-nbgl JJ cord-293913-frkb8iso 141 18 ( ( -LRB- cord-293913-frkb8iso 141 19 Lane lane NN cord-293913-frkb8iso 141 20 2 2 CD cord-293913-frkb8iso 141 21 ) ) -RRB- cord-293913-frkb8iso 141 22 in in IN cord-293913-frkb8iso 141 23 the the DT cord-293913-frkb8iso 141 24 presence presence NN cord-293913-frkb8iso 141 25 of of IN cord-293913-frkb8iso 141 26 [ [ -LRB- cord-293913-frkb8iso 141 27 3SS]methionine 3SS]methionine NNP cord-293913-frkb8iso 141 28 as as IN cord-293913-frkb8iso 141 29 a a DT cord-293913-frkb8iso 141 30 control control NN cord-293913-frkb8iso 141 31 for for IN cord-293913-frkb8iso 141 32 parallel parallel JJ cord-293913-frkb8iso 141 33 unlabeled unlabeled JJ cord-293913-frkb8iso 141 34 translation translation NN cord-293913-frkb8iso 141 35 reactions reaction NNS cord-293913-frkb8iso 141 36 . . . cord-293913-frkb8iso 142 1 Products product NNS cord-293913-frkb8iso 142 2 were be VBD cord-293913-frkb8iso 142 3 analyzed analyze VBN cord-293913-frkb8iso 142 4 on on IN cord-293913-frkb8iso 142 5 a a DT cord-293913-frkb8iso 142 6 10 10 CD cord-293913-frkb8iso 142 7 % % NN cord-293913-frkb8iso 142 8 SDS sds NN cord-293913-frkb8iso 142 9 - - HYPH cord-293913-frkb8iso 142 10 polyacrylamide polyacrylamide NN cord-293913-frkb8iso 142 11 gel gel NN cord-293913-frkb8iso 142 12 and and CC cord-293913-frkb8iso 142 13 visualized visualize VBN cord-293913-frkb8iso 142 14 by by IN cord-293913-frkb8iso 142 15 autoradiography autoradiography NNP cord-293913-frkb8iso 142 16 . . . cord-293913-frkb8iso 143 1 The the DT cord-293913-frkb8iso 143 2 position position NN cord-293913-frkb8iso 143 3 of of IN cord-293913-frkb8iso 143 4 the the DT cord-293913-frkb8iso 143 5 p28 p28 CD cord-293913-frkb8iso 143 6 c c NN cord-293913-frkb8iso 143 7 / / SYM cord-293913-frkb8iso 143 8 s s NN cord-293913-frkb8iso 143 9 - - HYPH cord-293913-frkb8iso 143 10 cleavage cleavage NN cord-293913-frkb8iso 143 11 product product NN cord-293913-frkb8iso 143 12 is be VBZ cord-293913-frkb8iso 143 13 indicated indicate VBN cord-293913-frkb8iso 143 14 by by IN cord-293913-frkb8iso 143 15 the the DT cord-293913-frkb8iso 143 16 arrow arrow NN cord-293913-frkb8iso 143 17 on on IN cord-293913-frkb8iso 143 18 the the DT cord-293913-frkb8iso 143 19 right right NN cord-293913-frkb8iso 143 20 . . . cord-293913-frkb8iso 144 1 cently cently RB cord-293913-frkb8iso 144 2 , , , cord-293913-frkb8iso 144 3 the the DT cord-293913-frkb8iso 144 4 activity activity NN cord-293913-frkb8iso 144 5 of of IN cord-293913-frkb8iso 144 6 the the DT cord-293913-frkb8iso 144 7 coronavirus coronavirus NN cord-293913-frkb8iso 144 8 3C 3c NN cord-293913-frkb8iso 144 9 - - HYPH cord-293913-frkb8iso 144 10 pro pro JJ cord-293913-frkb8iso 144 11 domain domain NN cord-293913-frkb8iso 144 12 has have VBZ cord-293913-frkb8iso 144 13 been be VBN cord-293913-frkb8iso 144 14 identified identify VBN cord-293913-frkb8iso 144 15 for for IN cord-293913-frkb8iso 144 16 MHV MHV NNP cord-293913-frkb8iso 144 17 - - HYPH cord-293913-frkb8iso 144 18 A59 A59 NNP cord-293913-frkb8iso 144 19 , , , cord-293913-frkb8iso 144 20 human human JJ cord-293913-frkb8iso 144 21 coronavirus coronavirus NN cord-293913-frkb8iso 144 22 229-E 229-e CD cord-293913-frkb8iso 144 23 ( ( -LRB- cord-293913-frkb8iso 144 24 Ziebuhr Ziebuhr NNP cord-293913-frkb8iso 144 25 et et NNP cord-293913-frkb8iso 144 26 al al NNP cord-293913-frkb8iso 144 27 . . NNP cord-293913-frkb8iso 144 28 , , , cord-293913-frkb8iso 144 29 1995 1995 CD cord-293913-frkb8iso 144 30 ) ) -RRB- cord-293913-frkb8iso 144 31 and and CC cord-293913-frkb8iso 144 32 the the DT cord-293913-frkb8iso 144 33 avian avian JJ cord-293913-frkb8iso 144 34 coronavirus coronavirus NN cord-293913-frkb8iso 144 35 infectious infectious JJ cord-293913-frkb8iso 144 36 bronchitis bronchitis NN cord-293913-frkb8iso 144 37 virus virus NN cord-293913-frkb8iso 144 38 Tibbles Tibbles NNP cord-293913-frkb8iso 144 39 et et NNP cord-293913-frkb8iso 144 40 al al NNP cord-293913-frkb8iso 144 41 . . NNP cord-293913-frkb8iso 144 42 , , , cord-293913-frkb8iso 144 43 1996 1996 CD cord-293913-frkb8iso 144 44 ) ) -RRB- cord-293913-frkb8iso 144 45 . . . cord-293913-frkb8iso 145 1 Using use VBG cord-293913-frkb8iso 145 2 bacterial bacterial JJ cord-293913-frkb8iso 145 3 expression expression NN cord-293913-frkb8iso 145 4 vectors vector NNS cord-293913-frkb8iso 145 5 , , , cord-293913-frkb8iso 145 6 it -PRON- PRP cord-293913-frkb8iso 145 7 has have VBZ cord-293913-frkb8iso 145 8 been be VBN cord-293913-frkb8iso 145 9 shown show VBN cord-293913-frkb8iso 145 10 that that IN cord-293913-frkb8iso 145 11 the the DT cord-293913-frkb8iso 145 12 coronavirus coronavirus NN cord-293913-frkb8iso 145 13 3C 3c NN cord-293913-frkb8iso 145 14 - - HYPH cord-293913-frkb8iso 145 15 like like JJ cord-293913-frkb8iso 145 16 proteinases proteinases NNP cord-293913-frkb8iso 145 17 recognize recognize VBP cord-293913-frkb8iso 145 18 predicted predict VBN cord-293913-frkb8iso 145 19 cleavage cleavage NN cord-293913-frkb8iso 145 20 sites site NNS cord-293913-frkb8iso 145 21 , , , cord-293913-frkb8iso 145 22 Q Q NNP cord-293913-frkb8iso 145 23 / / SYM cord-293913-frkb8iso 145 24 S S NNP cord-293913-frkb8iso 145 25 for for IN cord-293913-frkb8iso 145 26 MHV MHV NNP cord-293913-frkb8iso 145 27 - - HYPH cord-293913-frkb8iso 145 28 A59 A59 NNP cord-293913-frkb8iso 145 29 and and CC cord-293913-frkb8iso 145 30 IBV IBV NNP cord-293913-frkb8iso 145 31 , , , cord-293913-frkb8iso 145 32 and and CC cord-293913-frkb8iso 145 33 Q Q NNP cord-293913-frkb8iso 145 34 / / , cord-293913-frkb8iso 145 35 A a NN cord-293913-frkb8iso 145 36 for for IN cord-293913-frkb8iso 145 37 229E 229e CD cord-293913-frkb8iso 145 38 , , , cord-293913-frkb8iso 145 39 to to TO cord-293913-frkb8iso 145 40 release release VB cord-293913-frkb8iso 145 41 the the DT cord-293913-frkb8iso 145 42 3C 3c NN cord-293913-frkb8iso 145 43 domain domain NN cord-293913-frkb8iso 145 44 from from IN cord-293913-frkb8iso 145 45 the the DT cord-293913-frkb8iso 145 46 polyprotein polyprotein JJ cord-293913-frkb8iso 145 47 precursor precursor NN cord-293913-frkb8iso 145 48 . . . cord-293913-frkb8iso 146 1 These these DT cord-293913-frkb8iso 146 2 cleavage cleavage NN cord-293913-frkb8iso 146 3 sites site NNS cord-293913-frkb8iso 146 4 are be VBP cord-293913-frkb8iso 146 5 consistent consistent JJ cord-293913-frkb8iso 146 6 with with IN cord-293913-frkb8iso 146 7 the the DT cord-293913-frkb8iso 146 8 consensus consensus NN cord-293913-frkb8iso 146 9 recognition recognition NN cord-293913-frkb8iso 146 10 sequence sequence NN cord-293913-frkb8iso 146 11 of of IN cord-293913-frkb8iso 146 12 Q Q NNP cord-293913-frkb8iso 146 13 , , , cord-293913-frkb8iso 146 14 E e NN cord-293913-frkb8iso 146 15 / / SYM cord-293913-frkb8iso 146 16 G G NNP cord-293913-frkb8iso 146 17 , , , cord-293913-frkb8iso 146 18 S S NNP cord-293913-frkb8iso 146 19 , , , cord-293913-frkb8iso 146 20 A A NNP cord-293913-frkb8iso 146 21 for for IN cord-293913-frkb8iso 146 22 3C 3C NNP cord-293913-frkb8iso 146 23 proteinases proteinases NNP cord-293913-frkb8iso 146 24 ( ( -LRB- cord-293913-frkb8iso 146 25 Dougherty Dougherty NNP cord-293913-frkb8iso 146 26 and and CC cord-293913-frkb8iso 146 27 Semler Semler NNP cord-293913-frkb8iso 146 28 , , , cord-293913-frkb8iso 146 29 1993 1993 CD cord-293913-frkb8iso 146 30 ; ; : cord-293913-frkb8iso 146 31 Krausslich Krausslich NNP cord-293913-frkb8iso 146 32 and and CC cord-293913-frkb8iso 146 33 Wimmer Wimmer NNP cord-293913-frkb8iso 146 34 , , , cord-293913-frkb8iso 146 35 1988 1988 CD cord-293913-frkb8iso 146 36 ; ; : cord-293913-frkb8iso 146 37 Palmenberg Palmenberg NNP cord-293913-frkb8iso 146 38 , , , cord-293913-frkb8iso 146 39 1990 1990 CD cord-293913-frkb8iso 146 40 ) ) -RRB- cord-293913-frkb8iso 146 41 . . . cord-293913-frkb8iso 147 1 The the DT cord-293913-frkb8iso 147 2 picornavirus picornavirus NNP cord-293913-frkb8iso 147 3 3C 3C NNP cord-293913-frkb8iso 147 4 proteinases proteinases NNP cord-293913-frkb8iso 147 5 recognize recognize VBP cord-293913-frkb8iso 147 6 multiple multiple JJ cord-293913-frkb8iso 147 7 sites site NNS cord-293913-frkb8iso 147 8 in in IN cord-293913-frkb8iso 147 9 their -PRON- PRP$ cord-293913-frkb8iso 147 10 polyprotein polyprotein NN cord-293913-frkb8iso 147 11 and and CC cord-293913-frkb8iso 147 12 cleave cleave VBP cord-293913-frkb8iso 147 13 both both DT cord-293913-frkb8iso 147 14 in in IN cord-293913-frkb8iso 147 15 cis cis NNP cord-293913-frkb8iso 147 16 and and CC cord-293913-frkb8iso 147 17 in in IN cord-293913-frkb8iso 147 18 trans trans NNP cord-293913-frkb8iso 147 19 . . . cord-293913-frkb8iso 148 1 The the DT cord-293913-frkb8iso 148 2 MHV MHV NNP cord-293913-frkb8iso 148 3 - - HYPH cord-293913-frkb8iso 148 4 JHM JHM NNP cord-293913-frkb8iso 148 5 3C 3C NNP cord-293913-frkb8iso 148 6 - - HYPH cord-293913-frkb8iso 148 7 pro pro NNP cord-293913-frkb8iso 148 8 may may MD cord-293913-frkb8iso 148 9 also also RB cord-293913-frkb8iso 148 10 have have VB cord-293913-frkb8iso 148 11 multiple multiple JJ cord-293913-frkb8iso 148 12 cleavage cleavage NN cord-293913-frkb8iso 148 13 sites site NNS cord-293913-frkb8iso 148 14 , , , cord-293913-frkb8iso 148 15 including include VBG cord-293913-frkb8iso 148 16 a a DT cord-293913-frkb8iso 148 17 putative putative JJ cord-293913-frkb8iso 148 18 E e NN cord-293913-frkb8iso 148 19 / / SYM cord-293913-frkb8iso 148 20 S s NN cord-293913-frkb8iso 148 21 cleavage cleavage NN cord-293913-frkb8iso 148 22 site site NN cord-293913-frkb8iso 148 23 in in IN cord-293913-frkb8iso 148 24 the the DT cord-293913-frkb8iso 148 25 region region NN cord-293913-frkb8iso 148 26 predicted predict VBN cord-293913-frkb8iso 148 27 for for IN cord-293913-frkb8iso 148 28 cleavage cleavage NN cord-293913-frkb8iso 148 29 of of IN cord-293913-frkb8iso 148 30 p72 p72 NN cord-293913-frkb8iso 148 31 . . . cord-293913-frkb8iso 149 1 We -PRON- PRP cord-293913-frkb8iso 149 2 are be VBP cord-293913-frkb8iso 149 3 currently currently RB cord-293913-frkb8iso 149 4 investigating investigate VBG cord-293913-frkb8iso 149 5 whether whether IN cord-293913-frkb8iso 149 6 the the DT cord-293913-frkb8iso 149 7 3C 3c NN cord-293913-frkb8iso 149 8 - - HYPH cord-293913-frkb8iso 149 9 pro pro JJ cord-293913-frkb8iso 149 10 domain domain NN cord-293913-frkb8iso 149 11 is be VBZ cord-293913-frkb8iso 149 12 in in IN cord-293913-frkb8iso 149 13 fact fact NN cord-293913-frkb8iso 149 14 responsible responsible JJ cord-293913-frkb8iso 149 15 for for IN cord-293913-frkb8iso 149 16 cleavage cleavage NN cord-293913-frkb8iso 149 17 of of IN cord-293913-frkb8iso 149 18 p72 p72 NN cord-293913-frkb8iso 149 19 or or CC cord-293913-frkb8iso 149 20 p65 p65 NN cord-293913-frkb8iso 149 21 , , , cord-293913-frkb8iso 149 22 or or CC cord-293913-frkb8iso 149 23 if if IN cord-293913-frkb8iso 149 24 an an DT cord-293913-frkb8iso 149 25 as as RB cord-293913-frkb8iso 149 26 yet yet RB cord-293913-frkb8iso 149 27 unidentified unidentified JJ cord-293913-frkb8iso 149 28 proteinase proteinase NN cord-293913-frkb8iso 149 29 domain domain NN cord-293913-frkb8iso 149 30 is be VBZ cord-293913-frkb8iso 149 31 involved involve VBN cord-293913-frkb8iso 149 32 in in IN cord-293913-frkb8iso 149 33 these these DT cord-293913-frkb8iso 149 34 processing processing NN cord-293913-frkb8iso 149 35 events event NNS cord-293913-frkb8iso 149 36 . . . cord-293913-frkb8iso 150 1 Identification identification NN cord-293913-frkb8iso 150 2 of of IN cord-293913-frkb8iso 150 3 a a DT cord-293913-frkb8iso 150 4 domain domain NN cord-293913-frkb8iso 150 5 required require VBN cord-293913-frkb8iso 150 6 for for IN cord-293913-frkb8iso 150 7 autoproteolytic autoproteolytic JJ cord-293913-frkb8iso 150 8 cleavage cleavage NN cord-293913-frkb8iso 150 9 of of IN cord-293913-frkb8iso 150 10 murine murine JJ cord-293913-frkb8iso 150 11 coronavirus coronavirus NN cord-293913-frkb8iso 150 12 gene gene NN cord-293913-frkb8iso 150 13 A A NNP cord-293913-frkb8iso 150 14 polyprotein polyprotein JJ cord-293913-frkb8iso 150 15 Identification identification NN cord-293913-frkb8iso 150 16 of of IN cord-293913-frkb8iso 150 17 the the DT cord-293913-frkb8iso 150 18 catalytic catalytic JJ cord-293913-frkb8iso 150 19 sites site NNS cord-293913-frkb8iso 150 20 of of IN cord-293913-frkb8iso 150 21 a a DT cord-293913-frkb8iso 150 22 papain papain NN cord-293913-frkb8iso 150 23 - - HYPH cord-293913-frkb8iso 150 24 like like JJ cord-293913-frkb8iso 150 25 cysteine cysteine NN cord-293913-frkb8iso 150 26 proteinase proteinase NN cord-293913-frkb8iso 150 27 of of IN cord-293913-frkb8iso 150 28 murine murine JJ cord-293913-frkb8iso 150 29 coronavirus coronavirus NN cord-293913-frkb8iso 150 30 Characterization characterization NN cord-293913-frkb8iso 150 31 of of IN cord-293913-frkb8iso 150 32 replicative replicative JJ cord-293913-frkb8iso 150 33 intermediate intermediate JJ cord-293913-frkb8iso 150 34 RNA RNA NNP cord-293913-frkb8iso 150 35 of of IN cord-293913-frkb8iso 150 36 mouse mouse NNP cord-293913-frkb8iso 150 37 hepatitis hepatitis NNP cord-293913-frkb8iso 150 38 virus virus NN cord-293913-frkb8iso 150 39 : : : cord-293913-frkb8iso 150 40 presence presence NN cord-293913-frkb8iso 150 41 of of IN cord-293913-frkb8iso 150 42 leader leader NN cord-293913-frkb8iso 150 43 RNA RNA NNP cord-293913-frkb8iso 150 44 sequences sequence NNS cord-293913-frkb8iso 150 45 on on IN cord-293913-frkb8iso 150 46 nascent nascent JJ cord-293913-frkb8iso 150 47 chains chain NNS cord-293913-frkb8iso 150 48 Mouse Mouse NNP cord-293913-frkb8iso 150 49 hepatitis hepatitis NN cord-293913-frkb8iso 150 50 virus virus NN cord-293913-frkb8iso 150 51 A59 A59 NNP cord-293913-frkb8iso 150 52 RNA RNA NNP cord-293913-frkb8iso 150 53 polymerase polymerase NN cord-293913-frkb8iso 150 54 gene gene NN cord-293913-frkb8iso 150 55 ORF ORF NNP cord-293913-frkb8iso 150 56 1 1 CD cord-293913-frkb8iso 151 1 a a LS cord-293913-frkb8iso 151 2 : : : cord-293913-frkb8iso 151 3 heterogeneity heterogeneity NN cord-293913-frkb8iso 151 4 among among IN cord-293913-frkb8iso 151 5 MHV MHV NNP cord-293913-frkb8iso 151 6 strains strain VBZ cord-293913-frkb8iso 151 7 Characterization characterization NN cord-293913-frkb8iso 151 8 of of IN cord-293913-frkb8iso 151 9 the the DT cord-293913-frkb8iso 151 10 leader leader NN cord-293913-frkb8iso 151 11 papain papain NN cord-293913-frkb8iso 151 12 - - HYPH cord-293913-frkb8iso 151 13 like like JJ cord-293913-frkb8iso 151 14 proteinase proteinase NN cord-293913-frkb8iso 151 15 of of IN cord-293913-frkb8iso 151 16 MHV MHV NNP cord-293913-frkb8iso 151 17 - - HYPH cord-293913-frkb8iso 151 18 A59 a59 NN cord-293913-frkb8iso 151 19 : : : cord-293913-frkb8iso 151 20 identification identification NN cord-293913-frkb8iso 151 21 of of IN cord-293913-frkb8iso 151 22 a a DT cord-293913-frkb8iso 151 23 new new JJ cord-293913-frkb8iso 151 24 in in FW cord-293913-frkb8iso 151 25 vitro vitro FW cord-293913-frkb8iso 151 26 cleavage cleavage NN cord-293913-frkb8iso 151 27 site site NN cord-293913-frkb8iso 152 1 The the DT cord-293913-frkb8iso 152 2 primary primary JJ cord-293913-frkb8iso 152 3 structure structure NN cord-293913-frkb8iso 152 4 and and CC cord-293913-frkb8iso 152 5 expression expression NN cord-293913-frkb8iso 152 6 of of IN cord-293913-frkb8iso 152 7 the the DT cord-293913-frkb8iso 152 8 second second JJ cord-293913-frkb8iso 152 9 open open JJ cord-293913-frkb8iso 152 10 reading reading NN cord-293913-frkb8iso 152 11 frame frame NN cord-293913-frkb8iso 152 12 of of IN cord-293913-frkb8iso 152 13 the the DT cord-293913-frkb8iso 152 14 polymerase polymerase NN cord-293913-frkb8iso 152 15 gene gene NN cord-293913-frkb8iso 152 16 of of IN cord-293913-frkb8iso 152 17 the the DT cord-293913-frkb8iso 152 18 coronavirus coronavirus NN cord-293913-frkb8iso 152 19 MHV MHV NNP cord-293913-frkb8iso 152 20 - - HYPH cord-293913-frkb8iso 152 21 A59 A59 NNP cord-293913-frkb8iso 152 22 ; ; : cord-293913-frkb8iso 152 23 a a DT cord-293913-frkb8iso 152 24 highly highly RB cord-293913-frkb8iso 152 25 conserved conserved JJ cord-293913-frkb8iso 152 26 polymerase polymerase NN cord-293913-frkb8iso 152 27 is be VBZ cord-293913-frkb8iso 152 28 expressed express VBN cord-293913-frkb8iso 152 29 by by IN cord-293913-frkb8iso 152 30 an an DT cord-293913-frkb8iso 152 31 efficient efficient JJ cord-293913-frkb8iso 152 32 ribosomal ribosomal JJ cord-293913-frkb8iso 152 33 frameshifting frameshifting NN cord-293913-frkb8iso 152 34 mechanism mechanism NN cord-293913-frkb8iso 153 1 An an DT cord-293913-frkb8iso 153 2 efficient efficient JJ cord-293913-frkb8iso 153 3 ribosomal ribosomal JJ cord-293913-frkb8iso 153 4 frame frame NN cord-293913-frkb8iso 153 5 - - HYPH cord-293913-frkb8iso 153 6 shifting shift VBG cord-293913-frkb8iso 153 7 signal signal NN cord-293913-frkb8iso 153 8 in in IN cord-293913-frkb8iso 153 9 the the DT cord-293913-frkb8iso 153 10 polymeraseencoding polymeraseencode VBG cord-293913-frkb8iso 153 11 region region NN cord-293913-frkb8iso 153 12 of of IN cord-293913-frkb8iso 153 13 the the DT cord-293913-frkb8iso 153 14 coronavirus coronavirus NN cord-293913-frkb8iso 153 15 IBV IBV NNP cord-293913-frkb8iso 153 16 Characterization characterization NN cord-293913-frkb8iso 153 17 of of IN cord-293913-frkb8iso 153 18 an an DT cord-293913-frkb8iso 153 19 efficient efficient JJ cord-293913-frkb8iso 153 20 coronavirus coronavirus NN cord-293913-frkb8iso 153 21 ribosomal ribosomal NN cord-293913-frkb8iso 153 22 frameshifting frameshifte VBG cord-293913-frkb8iso 153 23 signal signal NN cord-293913-frkb8iso 153 24 : : : cord-293913-frkb8iso 153 25 requirement requirement NN cord-293913-frkb8iso 153 26 for for IN cord-293913-frkb8iso 153 27 an an DT cord-293913-frkb8iso 153 28 RNA RNA NNP cord-293913-frkb8iso 153 29 pseudoknot pseudoknot NN cord-293913-frkb8iso 153 30 Identification identification NN cord-293913-frkb8iso 153 31 and and CC cord-293913-frkb8iso 153 32 characterization characterization NN cord-293913-frkb8iso 153 33 of of IN cord-293913-frkb8iso 153 34 a a DT cord-293913-frkb8iso 153 35 65 65 CD cord-293913-frkb8iso 153 36 kDa kDa NNP cord-293913-frkb8iso 153 37 protein protein NN cord-293913-frkb8iso 153 38 processed process VBN cord-293913-frkb8iso 153 39 from from IN cord-293913-frkb8iso 153 40 the the DT cord-293913-frkb8iso 153 41 gene gene NN cord-293913-frkb8iso 153 42 1 1 CD cord-293913-frkb8iso 153 43 polyprotein polyprotein NN cord-293913-frkb8iso 153 44 of of IN cord-293913-frkb8iso 153 45 the the DT cord-293913-frkb8iso 153 46 murine murine JJ cord-293913-frkb8iso 153 47 coronavirus coronavirus NN cord-293913-frkb8iso 153 48 MHV MHV NNP cord-293913-frkb8iso 153 49 - - HYPH cord-293913-frkb8iso 153 50 A59 A59 NNP cord-293913-frkb8iso 153 51 Translation translation NN cord-293913-frkb8iso 153 52 and and CC cord-293913-frkb8iso 153 53 processing processing NN cord-293913-frkb8iso 153 54 of of IN cord-293913-frkb8iso 153 55 mouse mouse NN cord-293913-frkb8iso 153 56 hepatitis hepatitis NNP cord-293913-frkb8iso 153 57 virus virus NN cord-293913-frkb8iso 153 58 virion virion NN cord-293913-frkb8iso 153 59 RNA RNA NNP cord-293913-frkb8iso 153 60 in in IN cord-293913-frkb8iso 153 61 a a DT cord-293913-frkb8iso 153 62 cell cell NN cord-293913-frkb8iso 153 63 - - HYPH cord-293913-frkb8iso 153 64 free free JJ cord-293913-frkb8iso 153 65 system system NN cord-293913-frkb8iso 153 66 Identification identification NN cord-293913-frkb8iso 153 67 of of IN cord-293913-frkb8iso 153 68 a a DT cord-293913-frkb8iso 153 69 putative putative JJ cord-293913-frkb8iso 153 70 polymerase polymerase NN cord-293913-frkb8iso 153 71 gene gene NN cord-293913-frkb8iso 153 72 product product NN cord-293913-frkb8iso 153 73 in in IN cord-293913-frkb8iso 153 74 cells cell NNS cord-293913-frkb8iso 153 75 infected infect VBN cord-293913-frkb8iso 153 76 with with IN cord-293913-frkb8iso 153 77 murine murine JJ cord-293913-frkb8iso 153 78 coronavirus coronavirus NN cord-293913-frkb8iso 153 79 A59 A59 NNP cord-293913-frkb8iso 153 80 Intracellular Intracellular NNP cord-293913-frkb8iso 153 81 processing processing NN cord-293913-frkb8iso 153 82 of of IN cord-293913-frkb8iso 153 83 the the DT cord-293913-frkb8iso 153 84 N n CD cord-293913-frkb8iso 153 85 - - HYPH cord-293913-frkb8iso 153 86 terminal terminal NN cord-293913-frkb8iso 153 87 ORF ORF NNP cord-293913-frkb8iso 153 88 la la NNP cord-293913-frkb8iso 153 89 proteins protein NNS cord-293913-frkb8iso 153 90 of of IN cord-293913-frkb8iso 153 91 the the DT cord-293913-frkb8iso 153 92 coronavirus coronavirus NN cord-293913-frkb8iso 153 93 MHV MHV NNP cord-293913-frkb8iso 153 94 - - HYPH cord-293913-frkb8iso 153 95 A59 A59 NNP cord-293913-frkb8iso 153 96 requires require VBZ cord-293913-frkb8iso 153 97 multiple multiple JJ cord-293913-frkb8iso 153 98 proteolytic proteolytic JJ cord-293913-frkb8iso 153 99 events event NNS cord-293913-frkb8iso 153 100 Expression expression NN cord-293913-frkb8iso 153 101 of of IN cord-293913-frkb8iso 153 102 virusencoded virusencode VBN cord-293913-frkb8iso 153 103 proteinases proteinases NNP cord-293913-frkb8iso 153 104 : : : cord-293913-frkb8iso 153 105 functional functional JJ cord-293913-frkb8iso 153 106 and and CC cord-293913-frkb8iso 153 107 structural structural JJ cord-293913-frkb8iso 153 108 similarities similarity NNS cord-293913-frkb8iso 153 109 with with IN cord-293913-frkb8iso 153 110 cellular cellular JJ cord-293913-frkb8iso 153 111 enzymes enzyme NNS cord-293913-frkb8iso 153 112 Determinants determinant NNS cord-293913-frkb8iso 153 113 of of IN cord-293913-frkb8iso 153 114 the the DT cord-293913-frkb8iso 153 115 p28 p28 CD cord-293913-frkb8iso 153 116 cleavage cleavage NN cord-293913-frkb8iso 153 117 site site NN cord-293913-frkb8iso 153 118 recognized recognize VBN cord-293913-frkb8iso 153 119 by by IN cord-293913-frkb8iso 153 120 the the DT cord-293913-frkb8iso 153 121 first first JJ cord-293913-frkb8iso 153 122 papain papain NN cord-293913-frkb8iso 153 123 - - HYPH cord-293913-frkb8iso 153 124 like like JJ cord-293913-frkb8iso 153 125 cysteine cysteine NN cord-293913-frkb8iso 153 126 proteinase proteinase NN cord-293913-frkb8iso 153 127 of of IN cord-293913-frkb8iso 153 128 murine murine JJ cord-293913-frkb8iso 153 129 coronavirus coronavirus NN cord-293913-frkb8iso 153 130 Coronavirus Coronavirus NNP cord-293913-frkb8iso 153 131 genome genome NN cord-293913-frkb8iso 153 132 : : : cord-293913-frkb8iso 153 133 prediction prediction NN cord-293913-frkb8iso 153 134 of of IN cord-293913-frkb8iso 153 135 putative putative JJ cord-293913-frkb8iso 153 136 functional functional JJ cord-293913-frkb8iso 153 137 domains domain NNS cord-293913-frkb8iso 153 138 in in IN cord-293913-frkb8iso 153 139 the the DT cord-293913-frkb8iso 153 140 non non JJ cord-293913-frkb8iso 153 141 - - JJ cord-293913-frkb8iso 153 142 structural structural JJ cord-293913-frkb8iso 153 143 polyprotein polyprotein NN cord-293913-frkb8iso 153 144 by by IN cord-293913-frkb8iso 153 145 comparative comparative JJ cord-293913-frkb8iso 153 146 amino amino NN cord-293913-frkb8iso 153 147 acid acid NN cord-293913-frkb8iso 153 148 sequence sequence NN cord-293913-frkb8iso 153 149 analysis analysis NN cord-293913-frkb8iso 153 150 Putative Putative NNP cord-293913-frkb8iso 153 151 papain papain NN cord-293913-frkb8iso 153 152 - - HYPH cord-293913-frkb8iso 153 153 related relate VBN cord-293913-frkb8iso 153 154 thiol thiol NN cord-293913-frkb8iso 153 155 proteases protease NNS cord-293913-frkb8iso 153 156 of of IN cord-293913-frkb8iso 153 157 positive positive JJ cord-293913-frkb8iso 153 158 - - HYPH cord-293913-frkb8iso 153 159 strand strand NN cord-293913-frkb8iso 153 160 RNA RNA NNP cord-293913-frkb8iso 153 161 viruses virus NNS cord-293913-frkb8iso 153 162 Eukaryotic eukaryotic JJ cord-293913-frkb8iso 153 163 proteins protein NNS cord-293913-frkb8iso 153 164 expressed express VBN cord-293913-frkb8iso 153 165 in in IN cord-293913-frkb8iso 153 166 Escherichia Escherichia NNP cord-293913-frkb8iso 153 167 coli coli NNS cord-293913-frkb8iso 153 168 : : : cord-293913-frkb8iso 153 169 an an DT cord-293913-frkb8iso 153 170 improved improved JJ cord-293913-frkb8iso 153 171 thrombin thrombin JJ cord-293913-frkb8iso 153 172 cleavage cleavage NN cord-293913-frkb8iso 153 173 and and CC cord-293913-frkb8iso 153 174 purification purification NN cord-293913-frkb8iso 153 175 procedure procedure NN cord-293913-frkb8iso 153 176 of of IN cord-293913-frkb8iso 153 177 fusion fusion NN cord-293913-frkb8iso 153 178 proteins protein NNS cord-293913-frkb8iso 153 179 with with IN cord-293913-frkb8iso 153 180 glutathione glutathione NN cord-293913-frkb8iso 153 181 S s NN cord-293913-frkb8iso 153 182 - - HYPH cord-293913-frkb8iso 153 183 transferase transferase NN cord-293913-frkb8iso 153 184 Identification identification NN cord-293913-frkb8iso 153 185 of of IN cord-293913-frkb8iso 153 186 the the DT cord-293913-frkb8iso 153 187 murine murine JJ cord-293913-frkb8iso 153 188 coronavirus coronavirus NN cord-293913-frkb8iso 153 189 p28 p28 CD cord-293913-frkb8iso 153 190 cleavage cleavage NN cord-293913-frkb8iso 153 191 site site NN cord-293913-frkb8iso 154 1 Coronavirus Coronavirus NNP cord-293913-frkb8iso 154 2 protein protein NN cord-293913-frkb8iso 154 3 processing processing NN cord-293913-frkb8iso 154 4 and and CC cord-293913-frkb8iso 154 5 RNA RNA NNP cord-293913-frkb8iso 154 6 synthesis synthesis NN cord-293913-frkb8iso 154 7 is be VBZ cord-293913-frkb8iso 154 8 inhibited inhibit VBN cord-293913-frkb8iso 154 9 by by IN cord-293913-frkb8iso 154 10 the the DT cord-293913-frkb8iso 154 11 cysteine cysteine NN cord-293913-frkb8iso 154 12 proteinase proteinase NN cord-293913-frkb8iso 154 13 inhibitor inhibitor NN cord-293913-frkb8iso 154 14 E64d e64d NN cord-293913-frkb8iso 154 15 Viral Viral NNP cord-293913-frkb8iso 154 16 proteinases proteinases NNP cord-293913-frkb8iso 154 17 Coronavirus Coronavirus NNP cord-293913-frkb8iso 154 18 : : : cord-293913-frkb8iso 154 19 How how WRB cord-293913-frkb8iso 154 20 a a DT cord-293913-frkb8iso 154 21 large large JJ cord-293913-frkb8iso 154 22 RNA RNA NNP cord-293913-frkb8iso 154 23 viral viral JJ cord-293913-frkb8iso 154 24 genome genome NN cord-293913-frkb8iso 154 25 is be VBZ cord-293913-frkb8iso 154 26 replicated replicate VBN cord-293913-frkb8iso 154 27 and and CC cord-293913-frkb8iso 154 28 transcribed transcribe VBN cord-293913-frkb8iso 154 29 The the DT cord-293913-frkb8iso 154 30 complete complete JJ cord-293913-frkb8iso 154 31 sequence sequence NN cord-293913-frkb8iso 154 32 ( ( -LRB- cord-293913-frkb8iso 154 33 22 22 CD cord-293913-frkb8iso 154 34 kilobases kilobase NNS cord-293913-frkb8iso 154 35 ) ) -RRB- cord-293913-frkb8iso 154 36 of of IN cord-293913-frkb8iso 154 37 murine murine JJ cord-293913-frkb8iso 154 38 coronavirus coronavirus NN cord-293913-frkb8iso 154 39 gene gene NN cord-293913-frkb8iso 154 40 I -PRON- PRP cord-293913-frkb8iso 154 41 encoding encode VBG cord-293913-frkb8iso 154 42 the the DT cord-293913-frkb8iso 154 43 putative putative JJ cord-293913-frkb8iso 154 44 proteases protease NNS cord-293913-frkb8iso 154 45 and and CC cord-293913-frkb8iso 154 46 RNA RNA NNP cord-293913-frkb8iso 154 47 polymerase polymerase NN cord-293913-frkb8iso 154 48 Characterization characterization NN cord-293913-frkb8iso 154 49 and and CC cord-293913-frkb8iso 154 50 mutational mutational JJ cord-293913-frkb8iso 154 51 analysis analysis NN cord-293913-frkb8iso 154 52 of of IN cord-293913-frkb8iso 154 53 an an DT cord-293913-frkb8iso 154 54 ORF ORF NNP cord-293913-frkb8iso 154 55 la la NN cord-293913-frkb8iso 154 56 - - HYPH cord-293913-frkb8iso 154 57 encoding encode VBG cord-293913-frkb8iso 154 58 proteinase proteinase NN cord-293913-frkb8iso 154 59 domain domain NN cord-293913-frkb8iso 154 60 responsible responsible JJ cord-293913-frkb8iso 154 61 for for IN cord-293913-frkb8iso 154 62 proteolytic proteolytic JJ cord-293913-frkb8iso 154 63 processing processing NN cord-293913-frkb8iso 154 64 of of IN cord-293913-frkb8iso 154 65 the the DT cord-293913-frkb8iso 154 66 infectious infectious JJ cord-293913-frkb8iso 154 67 bronchitis bronchitis NN cord-293913-frkb8iso 154 68 virus virus NN cord-293913-frkb8iso 154 69 la la NNP cord-293913-frkb8iso 154 70 / / SYM cord-293913-frkb8iso 154 71 lb lb NNP cord-293913-frkb8iso 154 72 polyprotein polyprotein NNP cord-293913-frkb8iso 154 73 Identification Identification NNP cord-293913-frkb8iso 154 74 , , , cord-293913-frkb8iso 154 75 expression expression NN cord-293913-frkb8iso 154 76 , , , cord-293913-frkb8iso 154 77 and and CC cord-293913-frkb8iso 154 78 processing processing NN cord-293913-frkb8iso 154 79 of of IN cord-293913-frkb8iso 154 80 an an DT cord-293913-frkb8iso 154 81 87-kDa 87-kda CD cord-293913-frkb8iso 154 82 polypeptide polypeptide NN cord-293913-frkb8iso 154 83 encoded encode VBN cord-293913-frkb8iso 154 84 by by IN cord-293913-frkb8iso 154 85 ORF ORF NNP cord-293913-frkb8iso 154 86 la la NNP cord-293913-frkb8iso 154 87 of of IN cord-293913-frkb8iso 154 88 the the DT cord-293913-frkb8iso 154 89 coronavirus coronavirus NN cord-293913-frkb8iso 154 90 infectious infectious JJ cord-293913-frkb8iso 154 91 bronchitis bronchitis NN cord-293913-frkb8iso 154 92 virus virus NN cord-293913-frkb8iso 154 93 Identification identification NN cord-293913-frkb8iso 154 94 and and CC cord-293913-frkb8iso 154 95 characterization characterization NN cord-293913-frkb8iso 154 96 of of IN cord-293913-frkb8iso 154 97 a a DT cord-293913-frkb8iso 154 98 serine serine NN cord-293913-frkb8iso 154 99 - - HYPH cord-293913-frkb8iso 154 100 like like JJ cord-293913-frkb8iso 154 101 proteinase proteinase NN cord-293913-frkb8iso 154 102 of of IN cord-293913-frkb8iso 154 103 the the DT cord-293913-frkb8iso 154 104 murine murine JJ cord-293913-frkb8iso 154 105 coronavirus coronavirus NN cord-293913-frkb8iso 154 106 MHV MHV NNP cord-293913-frkb8iso 154 107 - - HYPH cord-293913-frkb8iso 154 108 A59 A59 NNP cord-293913-frkb8iso 154 109 High High NNP cord-293913-frkb8iso 154 110 frequency frequency NN cord-293913-frkb8iso 154 111 RNA rna NN cord-293913-frkb8iso 154 112 recombination recombination NN cord-293913-frkb8iso 154 113 of of IN cord-293913-frkb8iso 154 114 murine murine JJ cord-293913-frkb8iso 154 115 coronaviruses coronaviruse NNS cord-293913-frkb8iso 155 1 Proteolytic proteolytic JJ cord-293913-frkb8iso 155 2 processing processing NN cord-293913-frkb8iso 155 3 of of IN cord-293913-frkb8iso 155 4 picornaviral picornaviral NNP cord-293913-frkb8iso 155 5 polyprotein polyprotein NNP cord-293913-frkb8iso 155 6 Molecular Molecular NNP cord-293913-frkb8iso 155 7 cloning clone VBG cord-293913-frkb8iso 155 8 of of IN cord-293913-frkb8iso 155 9 the the DT cord-293913-frkb8iso 155 10 gene gene NN cord-293913-frkb8iso 155 11 encoding encode VBG cord-293913-frkb8iso 155 12 the the DT cord-293913-frkb8iso 155 13 putative putative JJ cord-293913-frkb8iso 155 14 polymerase polymerase NN cord-293913-frkb8iso 155 15 of of IN cord-293913-frkb8iso 155 16 mouse mouse NN cord-293913-frkb8iso 155 17 hepatitis hepatitis NN cord-293913-frkb8iso 155 18 coronavirus coronavirus NN cord-293913-frkb8iso 155 19 , , , cord-293913-frkb8iso 155 20 strain strain VB cord-293913-frkb8iso 155 21 A59 A59 NNP cord-293913-frkb8iso 155 22 Sequence sequence NN cord-293913-frkb8iso 155 23 and and CC cord-293913-frkb8iso 155 24 translation translation NN cord-293913-frkb8iso 155 25 of of IN cord-293913-frkb8iso 155 26 the the DT cord-293913-frkb8iso 155 27 murine murine JJ cord-293913-frkb8iso 155 28 coronavirus coronavirus NN cord-293913-frkb8iso 156 1 5'-end 5'-end CD cord-293913-frkb8iso 156 2 genomic genomic JJ cord-293913-frkb8iso 156 3 RNA RNA NNP cord-293913-frkb8iso 156 4 reveals reveal VBZ cord-293913-frkb8iso 156 5 the the DT cord-293913-frkb8iso 156 6 N n CD cord-293913-frkb8iso 156 7 - - HYPH cord-293913-frkb8iso 156 8 terminal terminal JJ cord-293913-frkb8iso 156 9 structure structure NN cord-293913-frkb8iso 156 10 of of IN cord-293913-frkb8iso 156 11 the the DT cord-293913-frkb8iso 156 12 putative putative JJ cord-293913-frkb8iso 156 13 RNA RNA NNP cord-293913-frkb8iso 156 14 polymerase polymerase NN cord-293913-frkb8iso 156 15 Coronavirus Coronavirus NNP cord-293913-frkb8iso 156 16 mRNA mRNA NNP cord-293913-frkb8iso 156 17 synthesis synthesis NN cord-293913-frkb8iso 156 18 involves involve VBZ cord-293913-frkb8iso 156 19 fusion fusion NN cord-293913-frkb8iso 156 20 of of IN cord-293913-frkb8iso 156 21 non non JJ cord-293913-frkb8iso 156 22 - - JJ cord-293913-frkb8iso 156 23 contiguous contiguous JJ cord-293913-frkb8iso 156 24 sequences sequence NNS cord-293913-frkb8iso 156 25 Characterization characterization NN cord-293913-frkb8iso 156 26 in in IN cord-293913-frkb8iso 156 27 vitro vitro FW cord-293913-frkb8iso 156 28 of of IN cord-293913-frkb8iso 156 29 an an DT cord-293913-frkb8iso 156 30 autocatalytic autocatalytic JJ cord-293913-frkb8iso 156 31 processing processing NN cord-293913-frkb8iso 156 32 activity activity NN cord-293913-frkb8iso 156 33 associated associate VBN cord-293913-frkb8iso 156 34 with with IN cord-293913-frkb8iso 156 35 the the DT cord-293913-frkb8iso 156 36 predicted predict VBN cord-293913-frkb8iso 156 37 3C 3c NN cord-293913-frkb8iso 156 38 - - HYPH cord-293913-frkb8iso 156 39 like like JJ cord-293913-frkb8iso 156 40 proteinase proteinase NN cord-293913-frkb8iso 156 41 domain domain NN cord-293913-frkb8iso 156 42 of of IN cord-293913-frkb8iso 156 43 the the DT cord-293913-frkb8iso 156 44 coronavirus coronavirus NN cord-293913-frkb8iso 156 45 avian avian JJ cord-293913-frkb8iso 156 46 infectious infectious JJ cord-293913-frkb8iso 156 47 bronchitis bronchitis NN cord-293913-frkb8iso 156 48 virus virus NN cord-293913-frkb8iso 156 49 Characterization characterization NN cord-293913-frkb8iso 156 50 of of IN cord-293913-frkb8iso 156 51 a a DT cord-293913-frkb8iso 156 52 human human JJ cord-293913-frkb8iso 156 53 coronavirus coronavirus NN cord-293913-frkb8iso 156 54 ( ( -LRB- cord-293913-frkb8iso 156 55 strain strain NN cord-293913-frkb8iso 156 56 229E 229e CD cord-293913-frkb8iso 156 57 ) ) -RRB- cord-293913-frkb8iso 157 1 3C 3C NNP cord-293913-frkb8iso 157 2 - - HYPH cord-293913-frkb8iso 157 3 like like JJ cord-293913-frkb8iso 157 4 proteinase proteinase NN cord-293913-frkb8iso 157 5 activity activity NN cord-293913-frkb8iso 158 1 This this DT cord-293913-frkb8iso 158 2 research research NN cord-293913-frkb8iso 158 3 was be VBD cord-293913-frkb8iso 158 4 supported support VBN cord-293913-frkb8iso 158 5 by by IN cord-293913-frkb8iso 158 6 Public Public NNP cord-293913-frkb8iso 158 7 Health Health NNP cord-293913-frkb8iso 158 8 Service Service NNP cord-293913-frkb8iso 158 9 Research Research NNP cord-293913-frkb8iso 158 10 Grant Grant NNP cord-293913-frkb8iso 158 11 AI32065 AI32065 NNP cord-293913-frkb8iso 158 12 and and CC cord-293913-frkb8iso 158 13 a a DT cord-293913-frkb8iso 158 14 Junior Junior NNP cord-293913-frkb8iso 158 15 Faculty Faculty NNP cord-293913-frkb8iso 158 16 Research Research NNP cord-293913-frkb8iso 158 17 Award Award NNP cord-293913-frkb8iso 158 18 ( ( -LRB- cord-293913-frkb8iso 158 19 to to IN cord-293913-frkb8iso 158 20 S.C.B. S.C.B. NNP cord-293913-frkb8iso 158 21 ) ) -RRB- cord-293913-frkb8iso 158 22 from from IN cord-293913-frkb8iso 158 23 the the DT cord-293913-frkb8iso 158 24 American American NNP cord-293913-frkb8iso 158 25 Cancer Cancer NNP cord-293913-frkb8iso 158 26 Society Society NNP cord-293913-frkb8iso 158 27 . . .