id author title date pages extension mime words sentences flesch summary cache txt cord-338165-mgrf1odm Wang, Xiaojing CD4–CD8-T cells contribute to the persistence of viral hepatitis by striking a delicate balance in immune modulation 2012-11-30 .txt text/plain 5021 272 54 Virus titers were determined in the liver tissue of MHV-3 infected C3H/HeJ mice at various time points by standard plaque assay as described before [32] . Cell suspensions of processed spleens from C3H/HeJ mice on days 4, 10, 15, 20, and 30 post MHV-3 infection were stained with PEcy5.5-anti-CD3, FITC-anti-CD4, FITC-anti-CD8, APC-anti-CD25, APC-anti-TCRb, PE-anti-TCRcd, PE-anti-CD28/PE-anti-CD30, PE-anti-CD44, PE-anti-CD95L, PEanti-CD95 monoclonal antibodies or isotype control Ab (eBioscience, San Diego, CA, USA). Adoptive transfer of DN T cells from MHV-3 infected C3H/HeJ mice increased the survival rate and improved liver histology of recipient mice infected by the same virus strain but had little impact on the virus titer of liver tissue Fig. 3A showed that no significant difference in virus titer of liver tissue was observed among DN T cells group, splenocytes group, DNT-depleted splenocytes group and PBS control group. ./cache/cord-338165-mgrf1odm.txt ./txt/cord-338165-mgrf1odm.txt