id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312294-c9e18rai	Daniel, Claude	Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6	1994-12-31		.txt	text/plain	2175	111	47	title: Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6 Abstract Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immuno-precipitation and enzyme-linked immunosorbent assays. In this study, we show that infection by MHV-A59 in culture medium at pH 6, instead of the usual pH 7, not only increased infectious viral titers, but also greatly enhanced the reactivity of the spike glycoprotein to antibodies. Metabolically labeled virus-infected cell lysates were immunoprecipitated with MHV-specific antibodies ((Y-AS9: hyperimmune serum; 7-10A: anti-S MAb; 653: control ascites fluid) and resolved by SDS-PAGE on a 7-15% gel. Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion	./cache/cord-312294-c9e18rai.txt	./txt/cord-312294-c9e18rai.txt