id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292424-daj4zcm1	Shang, Jian	Structure of mouse coronavirus spike protein complexed with receptor reveals mechanism for viral entry	2020-03-09		.txt	text/plain	6780	361	56	Together these results reveal a new role of receptor binding in MHV entry: in addition to its well-characterized role in viral attachment to host cells, receptor binding also induces the conformational change of the spike and hence the fusion of viral and host membranes. In this study, we determined the cryo-electron microscopic (cryo-EM) structure of pre-fusion MHV spike in complex with CEACAM1a (D1-D4), which reveals the structural change of MHV spike associated with receptor binding. Using proteolysis and negative-stain EM assays, we further investigated the impact of receptor binding on proteases sensitivity and the final structural transitions of MHV spike. The result showed that receptor treatment of the trypsin-cleaved MHV S-e led to a protease K-resistant S2' fragment, suggesting that CEACAM1a binding facilitated the already cleaved MHV S-e to transition from pre-fusion to post-fusion conformation.	./cache/cord-292424-daj4zcm1.txt	./txt/cord-292424-daj4zcm1.txt