id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-271763-cual2qv4	Abraham, Sushma	Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site	1990-05-31		.txt	text/plain	2814	334	68	Abstract The sequence of the spike (also called peplomer or E2) protein gene of the Mebus strain of bovine coronavirus (BCV) was obtained from cDNA clones of genomic RNA. Amino terminal amino acid sequencing of the virion-derived gp100 spike subunit confirmed the location of the predicted cleavage site, and established that gp120 and gp100 are the glycosylated virion forms of the S1 and S2 subunits, respectively. The deduced amino acid sequence of the extended ORF demonstrated high sequence similarity to the C-terminal end of the antigenically related MHV-A59 (22) and MHV-JHM (32) S proteins, both antigenic homologs of the BCV S protein (13). The N-terminal sequence of the lOO-kDa subunit could be obtained, however, and was determined to be X-l-T-T-G-Y-X-F-, identifying the first amino acids downstream from the predicted internal cleavage site.	./cache/cord-271763-cual2qv4.txt	./txt/cord-271763-cual2qv4.txt