id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-268139-tgpsu4qz	Brockway, Sarah M.	Mutagenesis of the murine hepatitis virus nsp1-coding region identifies residues important for protein processing, viral RNA synthesis, and viral replication	2005-09-30		.txt	text/plain	10127	558	54	title: Mutagenesis of the murine hepatitis virus nsp1-coding region identifies residues important for protein processing, viral RNA synthesis, and viral replication In the current study, a reverse genetic approach was used to define the role of the 28-kDa amino-terminal product (nsp1) of the gene 1 polyprotein during replication of the coronavirus murine hepatitis virus (MHV) in cell culture. To determine whether nsp1 is required for MHV replication and to identify residues critical for protein function, mutant viruses that contained deletions or point mutations within the nsp1-coding region were generated and assayed for defects in viral replication, viral protein expression, protein localization, and RNA synthesis. Mutagenesis of the murine hepatitis virus nsp1-coding region identifies residues important for protein processing, viral RNA synthesis, and viral replication Experiments were next performed to determine if viral gene expression occurred in cells electroporated with RNA for mutants that did not produce infectious virus (VUSB5, VUSB6, nsp1DFL, and nsp1DMid).	./cache/cord-268139-tgpsu4qz.txt	./txt/cord-268139-tgpsu4qz.txt