id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-252882-2qhoqa88	Hingley, Susan T.	The virulence of mouse hepatitis virus strain A59 is not dependent on efficient spike protein cleavage and cell-to-cell fusion	2002		.txt	text/plain	5915	271	53	Targeted recombination was used to introduce amino acid substitutions into the cleavage signal of the fusion glycoprotein (spike or S protein) of MHV strain A59. The mutant cleavage and fusion phenotypes were seen only when the H716D substitution was present (Hingley et al, 1995; Leparc-Goffart et al, 1997 ; however, none of the glial cell mutants had the H716D mutation alone, so it was necessary to isolate recombinant viruses expressing the H716D mutation alone in order to assess the potential affect of this particular mutation on cleavage of S, fusogenicity, and virulence. Previous studies from this laboratory using mutants isolated from persistently infected glial cells (Gombold et al, 1993) have suggested that an H716D mutation, which changes the RRAHR cleavage signal to an RRADR sequence, is associated with an uncleaved S protein, but did not directly assess the affect of that mutation on virulence.	./cache/cord-252882-2qhoqa88.txt	./txt/cord-252882-2qhoqa88.txt