id sid tid token lemma pos cord-323428-jd91k19z 1 1 key key JJ cord-323428-jd91k19z 2 1 : : : cord-323428-jd91k19z 2 2 cord-323428-jd91k19z cord-323428-jd91k19z NN cord-323428-jd91k19z 2 3 authors author NNS cord-323428-jd91k19z 2 4 : : : cord-323428-jd91k19z 2 5 Ababneh Ababneh NNP cord-323428-jd91k19z 2 6 , , , cord-323428-jd91k19z 2 7 Mustafa Mustafa NNP cord-323428-jd91k19z 2 8 ; ; : cord-323428-jd91k19z 2 9 Alrwashdeh Alrwashdeh NNP cord-323428-jd91k19z 2 10 , , , cord-323428-jd91k19z 2 11 Mu’men Mu’men NNPS cord-323428-jd91k19z 2 12 ; ; : cord-323428-jd91k19z 3 1 Khalifeh Khalifeh NNP cord-323428-jd91k19z 3 2 , , , cord-323428-jd91k19z 3 3 Mohammad Mohammad NNP cord-323428-jd91k19z 3 4 title title NN cord-323428-jd91k19z 3 5 : : : cord-323428-jd91k19z 3 6 Recombinant recombinant JJ cord-323428-jd91k19z 3 7 adenoviral adenoviral JJ cord-323428-jd91k19z 3 8 vaccine vaccine NN cord-323428-jd91k19z 3 9 encoding encode VBG cord-323428-jd91k19z 3 10 the the DT cord-323428-jd91k19z 3 11 spike spike NN cord-323428-jd91k19z 3 12 1 1 CD cord-323428-jd91k19z 3 13 subunit subunit NN cord-323428-jd91k19z 3 14 of of IN cord-323428-jd91k19z 3 15 the the DT cord-323428-jd91k19z 3 16 Middle Middle NNP cord-323428-jd91k19z 3 17 East East NNP cord-323428-jd91k19z 3 18 Respiratory Respiratory NNP cord-323428-jd91k19z 3 19 Syndrome Syndrome NNP cord-323428-jd91k19z 3 20 Coronavirus Coronavirus NNP cord-323428-jd91k19z 3 21 elicits elicit VBZ cord-323428-jd91k19z 3 22 strong strong JJ cord-323428-jd91k19z 3 23 humoral humoral JJ cord-323428-jd91k19z 3 24 and and CC cord-323428-jd91k19z 3 25 cellular cellular JJ cord-323428-jd91k19z 3 26 immune immune JJ cord-323428-jd91k19z 3 27 responses response NNS cord-323428-jd91k19z 3 28 in in IN cord-323428-jd91k19z 3 29 mice mouse NNS cord-323428-jd91k19z 3 30 date date NN cord-323428-jd91k19z 3 31 : : : cord-323428-jd91k19z 3 32 2019 2019 CD cord-323428-jd91k19z 3 33 - - SYM cord-323428-jd91k19z 3 34 10 10 CD cord-323428-jd91k19z 3 35 - - SYM cord-323428-jd91k19z 3 36 11 11 CD cord-323428-jd91k19z 3 37 journal journal NN cord-323428-jd91k19z 3 38 : : : cord-323428-jd91k19z 3 39 Vet Vet NNP cord-323428-jd91k19z 3 40 World World NNP cord-323428-jd91k19z 3 41 DOI doi NN cord-323428-jd91k19z 3 42 : : : cord-323428-jd91k19z 3 43 10.14202 10.14202 CD cord-323428-jd91k19z 3 44 / / SYM cord-323428-jd91k19z 3 45 vetworld.2019.1554 vetworld.2019.1554 NNS cord-323428-jd91k19z 3 46 - - HYPH cord-323428-jd91k19z 3 47 1562 1562 CD cord-323428-jd91k19z 3 48 sha sha NNP cord-323428-jd91k19z 3 49 : : : cord-323428-jd91k19z 3 50 3c9db7ee30074667f9fe308de9910b013aa1f163 3c9db7ee30074667f9fe308de9910b013aa1f163 CD cord-323428-jd91k19z 3 51 doc_id doc_id CD cord-323428-jd91k19z 3 52 : : : cord-323428-jd91k19z 3 53 323428 323428 CD cord-323428-jd91k19z 3 54 cord_uid cord_uid NNS cord-323428-jd91k19z 3 55 : : : cord-323428-jd91k19z 3 56 jd91k19z jd91k19z NNP cord-323428-jd91k19z 3 57 BACKGROUND background NN cord-323428-jd91k19z 3 58 AND and CC cord-323428-jd91k19z 3 59 AIM aim VB cord-323428-jd91k19z 3 60 : : : cord-323428-jd91k19z 3 61 Middle Middle NNP cord-323428-jd91k19z 3 62 East East NNP cord-323428-jd91k19z 3 63 respiratory respiratory JJ cord-323428-jd91k19z 3 64 syndrome syndrome NN cord-323428-jd91k19z 3 65 coronavirus coronavirus NN cord-323428-jd91k19z 3 66 ( ( -LRB- cord-323428-jd91k19z 3 67 MERS MERS NNP cord-323428-jd91k19z 3 68 - - HYPH cord-323428-jd91k19z 3 69 CoV CoV NNP cord-323428-jd91k19z 3 70 ) ) -RRB- cord-323428-jd91k19z 3 71 has have VBZ cord-323428-jd91k19z 3 72 rapidly rapidly RB cord-323428-jd91k19z 3 73 spread spread VBN cord-323428-jd91k19z 3 74 throughout throughout IN cord-323428-jd91k19z 3 75 the the DT cord-323428-jd91k19z 3 76 Middle Middle NNP cord-323428-jd91k19z 3 77 East East NNP cord-323428-jd91k19z 3 78 since since IN cord-323428-jd91k19z 3 79 its -PRON- PRP$ cord-323428-jd91k19z 3 80 discovery discovery NN cord-323428-jd91k19z 3 81 in in IN cord-323428-jd91k19z 3 82 2012 2012 CD cord-323428-jd91k19z 3 83 . . . cord-323428-jd91k19z 4 1 The the DT cord-323428-jd91k19z 4 2 virus virus NN cord-323428-jd91k19z 4 3 poses pose VBZ cord-323428-jd91k19z 4 4 a a DT cord-323428-jd91k19z 4 5 significant significant JJ cord-323428-jd91k19z 4 6 global global JJ cord-323428-jd91k19z 4 7 public public JJ cord-323428-jd91k19z 4 8 health health NN cord-323428-jd91k19z 4 9 threat threat NN cord-323428-jd91k19z 4 10 with with IN cord-323428-jd91k19z 4 11 potentially potentially RB cord-323428-jd91k19z 4 12 devastating devastating JJ cord-323428-jd91k19z 4 13 effects effect NNS cord-323428-jd91k19z 4 14 . . . cord-323428-jd91k19z 5 1 In in IN cord-323428-jd91k19z 5 2 this this DT cord-323428-jd91k19z 5 3 study study NN cord-323428-jd91k19z 5 4 , , , cord-323428-jd91k19z 5 5 a a DT cord-323428-jd91k19z 5 6 recombinant recombinant JJ cord-323428-jd91k19z 5 7 adenoviral adenoviral NN cord-323428-jd91k19z 5 8 - - HYPH cord-323428-jd91k19z 5 9 based base VBN cord-323428-jd91k19z 5 10 vaccine vaccine NN cord-323428-jd91k19z 5 11 encoding encode VBG cord-323428-jd91k19z 5 12 the the DT cord-323428-jd91k19z 5 13 spike spike NN cord-323428-jd91k19z 5 14 1 1 CD cord-323428-jd91k19z 5 15 ( ( -LRB- cord-323428-jd91k19z 5 16 S1 S1 NNP cord-323428-jd91k19z 5 17 ) ) -RRB- cord-323428-jd91k19z 5 18 subunit subunit NN cord-323428-jd91k19z 5 19 of of IN cord-323428-jd91k19z 5 20 the the DT cord-323428-jd91k19z 5 21 MERS MERS NNP cord-323428-jd91k19z 5 22 - - HYPH cord-323428-jd91k19z 5 23 CoV CoV NNP cord-323428-jd91k19z 5 24 genome genome NN cord-323428-jd91k19z 5 25 was be VBD cord-323428-jd91k19z 5 26 constructed construct VBN cord-323428-jd91k19z 5 27 , , , cord-323428-jd91k19z 5 28 and and CC cord-323428-jd91k19z 5 29 its -PRON- PRP$ cord-323428-jd91k19z 5 30 humoral humoral JJ cord-323428-jd91k19z 5 31 , , , cord-323428-jd91k19z 5 32 and and CC cord-323428-jd91k19z 5 33 cellular cellular JJ cord-323428-jd91k19z 5 34 immune immune JJ cord-323428-jd91k19z 5 35 responses response NNS cord-323428-jd91k19z 5 36 were be VBD cord-323428-jd91k19z 5 37 evaluated evaluate VBN cord-323428-jd91k19z 5 38 in in IN cord-323428-jd91k19z 5 39 mice mouse NNS cord-323428-jd91k19z 5 40 . . . cord-323428-jd91k19z 6 1 MATERIALS materials NN cord-323428-jd91k19z 6 2 AND and CC cord-323428-jd91k19z 6 3 METHODS method NNS cord-323428-jd91k19z 6 4 : : : cord-323428-jd91k19z 7 1 Mice mouse NNS cord-323428-jd91k19z 7 2 were be VBD cord-323428-jd91k19z 7 3 immunized immunize VBN cord-323428-jd91k19z 7 4 initially initially RB cord-323428-jd91k19z 7 5 by by IN cord-323428-jd91k19z 7 6 intramuscular intramuscular JJ cord-323428-jd91k19z 7 7 injection injection NN cord-323428-jd91k19z 7 8 and and CC cord-323428-jd91k19z 7 9 boosted boost VBD cord-323428-jd91k19z 7 10 3 3 CD cord-323428-jd91k19z 7 11 weeks week NNS cord-323428-jd91k19z 7 12 later later RB cord-323428-jd91k19z 7 13 by by IN cord-323428-jd91k19z 7 14 intranasal intranasal NN cord-323428-jd91k19z 7 15 application application NN cord-323428-jd91k19z 7 16 . . . cord-323428-jd91k19z 8 1 Expression expression NN cord-323428-jd91k19z 8 2 of of IN cord-323428-jd91k19z 8 3 the the DT cord-323428-jd91k19z 8 4 S1 S1 NNP cord-323428-jd91k19z 8 5 protein protein NN cord-323428-jd91k19z 8 6 in in IN cord-323428-jd91k19z 8 7 the the DT cord-323428-jd91k19z 8 8 lungs lung NNS cord-323428-jd91k19z 8 9 and and CC cord-323428-jd91k19z 8 10 kidneys kidney NNS cord-323428-jd91k19z 8 11 was be VBD cord-323428-jd91k19z 8 12 detected detect VBN cord-323428-jd91k19z 8 13 using use VBG cord-323428-jd91k19z 8 14 conventional conventional JJ cord-323428-jd91k19z 8 15 polymerase polymerase NN cord-323428-jd91k19z 8 16 chain chain NN cord-323428-jd91k19z 8 17 reaction reaction NN cord-323428-jd91k19z 8 18 ( ( -LRB- cord-323428-jd91k19z 8 19 PCR PCR NNP cord-323428-jd91k19z 8 20 ) ) -RRB- cord-323428-jd91k19z 8 21 and and CC cord-323428-jd91k19z 8 22 immunohistochemistry immunohistochemistry JJ cord-323428-jd91k19z 8 23 ( ( -LRB- cord-323428-jd91k19z 8 24 IHC IHC NNP cord-323428-jd91k19z 8 25 ) ) -RRB- cord-323428-jd91k19z 8 26 targeting target VBG cord-323428-jd91k19z 8 27 specific specific JJ cord-323428-jd91k19z 8 28 regions region NNS cord-323428-jd91k19z 8 29 within within IN cord-323428-jd91k19z 8 30 the the DT cord-323428-jd91k19z 8 31 S1 S1 NNP cord-323428-jd91k19z 8 32 subunit subunit NN cord-323428-jd91k19z 8 33 at at IN cord-323428-jd91k19z 8 34 weeks week NNS cord-323428-jd91k19z 8 35 3 3 CD cord-323428-jd91k19z 8 36 , , , cord-323428-jd91k19z 8 37 4 4 CD cord-323428-jd91k19z 8 38 , , , cord-323428-jd91k19z 8 39 5 5 CD cord-323428-jd91k19z 8 40 , , , cord-323428-jd91k19z 8 41 and and CC cord-323428-jd91k19z 8 42 6 6 CD cord-323428-jd91k19z 8 43 after after IN cord-323428-jd91k19z 8 44 the the DT cord-323428-jd91k19z 8 45 first first JJ cord-323428-jd91k19z 8 46 vaccination vaccination NN cord-323428-jd91k19z 8 47 . . . cord-323428-jd91k19z 9 1 Antigen antigen NN cord-323428-jd91k19z 9 2 - - HYPH cord-323428-jd91k19z 9 3 specific specific JJ cord-323428-jd91k19z 9 4 humoral humoral JJ cord-323428-jd91k19z 9 5 and and CC cord-323428-jd91k19z 9 6 cellular cellular JJ cord-323428-jd91k19z 9 7 immune immune JJ cord-323428-jd91k19z 9 8 responses response NNS cord-323428-jd91k19z 9 9 were be VBD cord-323428-jd91k19z 9 10 evaluated evaluate VBN cord-323428-jd91k19z 9 11 in in IN cord-323428-jd91k19z 9 12 serum serum NN cord-323428-jd91k19z 9 13 and and CC cord-323428-jd91k19z 9 14 in in IN cord-323428-jd91k19z 9 15 cell cell NN cord-323428-jd91k19z 9 16 culture culture NN cord-323428-jd91k19z 9 17 following follow VBG cord-323428-jd91k19z 9 18 in in IN cord-323428-jd91k19z 9 19 vitro vitro FW cord-323428-jd91k19z 9 20 stimulation stimulation NN cord-323428-jd91k19z 9 21 with with IN cord-323428-jd91k19z 9 22 a a DT cord-323428-jd91k19z 9 23 specific specific JJ cord-323428-jd91k19z 9 24 9-mer 9-mer CD cord-323428-jd91k19z 9 25 epitope epitope NN cord-323428-jd91k19z 9 26 within within IN cord-323428-jd91k19z 9 27 the the DT cord-323428-jd91k19z 9 28 S1 s1 NN cord-323428-jd91k19z 9 29 protein protein NN cord-323428-jd91k19z 9 30 ( ( -LRB- cord-323428-jd91k19z 9 31 CYSSLILDY CYSSLILDY NNP cord-323428-jd91k19z 9 32 ) ) -RRB- cord-323428-jd91k19z 9 33 . . . cord-323428-jd91k19z 10 1 RESULTS result NNS cord-323428-jd91k19z 10 2 : : : cord-323428-jd91k19z 11 1 S1 s1 NN cord-323428-jd91k19z 11 2 protein protein NN cord-323428-jd91k19z 11 3 expression expression NN cord-323428-jd91k19z 11 4 was be VBD cord-323428-jd91k19z 11 5 only only RB cord-323428-jd91k19z 11 6 detected detect VBN cord-323428-jd91k19z 11 7 by by IN cord-323428-jd91k19z 11 8 IHC IHC NNP cord-323428-jd91k19z 11 9 in in IN cord-323428-jd91k19z 11 10 the the DT cord-323428-jd91k19z 11 11 kidneys kidney NNS cord-323428-jd91k19z 11 12 of of IN cord-323428-jd91k19z 11 13 the the DT cord-323428-jd91k19z 11 14 Ad Ad NNP cord-323428-jd91k19z 11 15 - - HYPH cord-323428-jd91k19z 11 16 MERS MERS NNP cord-323428-jd91k19z 11 17 - - HYPH cord-323428-jd91k19z 11 18 S1 S1 NNP cord-323428-jd91k19z 11 19 group group NN cord-323428-jd91k19z 11 20 at at IN cord-323428-jd91k19z 11 21 week week NN cord-323428-jd91k19z 11 22 6 6 CD cord-323428-jd91k19z 11 23 from from IN cord-323428-jd91k19z 11 24 first first JJ cord-323428-jd91k19z 11 25 immunization immunization NN cord-323428-jd91k19z 11 26 , , , cord-323428-jd91k19z 11 27 and and CC cord-323428-jd91k19z 11 28 in in IN cord-323428-jd91k19z 11 29 both both DT cord-323428-jd91k19z 11 30 lungs lung NNS cord-323428-jd91k19z 11 31 and and CC cord-323428-jd91k19z 11 32 kidneys kidney NNS cord-323428-jd91k19z 11 33 of of IN cord-323428-jd91k19z 11 34 Ad ad NN cord-323428-jd91k19z 11 35 - - HYPH cord-323428-jd91k19z 11 36 MERS MERS NNP cord-323428-jd91k19z 11 37 - - HYPH cord-323428-jd91k19z 11 38 S1 S1 NNP cord-323428-jd91k19z 11 39 group group NN cord-323428-jd91k19z 11 40 by by IN cord-323428-jd91k19z 11 41 conventional conventional JJ cord-323428-jd91k19z 11 42 PCR PCR NNP cord-323428-jd91k19z 11 43 at at IN cord-323428-jd91k19z 11 44 weeks week NNS cord-323428-jd91k19z 11 45 3 3 CD cord-323428-jd91k19z 11 46 and and CC cord-323428-jd91k19z 11 47 5 5 CD cord-323428-jd91k19z 11 48 post post JJ cord-323428-jd91k19z 11 49 - - JJ cord-323428-jd91k19z 11 50 prime prime JJ cord-323428-jd91k19z 11 51 . . . cord-323428-jd91k19z 12 1 The the DT cord-323428-jd91k19z 12 2 vaccine vaccine NN cord-323428-jd91k19z 12 3 elicited elicit VBD cord-323428-jd91k19z 12 4 a a DT cord-323428-jd91k19z 12 5 specific specific JJ cord-323428-jd91k19z 12 6 S1-immunoglobulin S1-immunoglobulin NNP cord-323428-jd91k19z 12 7 G G NNP cord-323428-jd91k19z 12 8 antibody antibody NN cord-323428-jd91k19z 12 9 response response NN cord-323428-jd91k19z 12 10 , , , cord-323428-jd91k19z 12 11 which which WDT cord-323428-jd91k19z 12 12 was be VBD cord-323428-jd91k19z 12 13 detected detect VBN cord-323428-jd91k19z 12 14 in in IN cord-323428-jd91k19z 12 15 the the DT cord-323428-jd91k19z 12 16 sera sera NNP cord-323428-jd91k19z 12 17 of of IN cord-323428-jd91k19z 12 18 the the DT cord-323428-jd91k19z 12 19 vaccinated vaccinated JJ cord-323428-jd91k19z 12 20 mice mouse NNS cord-323428-jd91k19z 12 21 at at IN cord-323428-jd91k19z 12 22 weeks week NNS cord-323428-jd91k19z 12 23 4 4 CD cord-323428-jd91k19z 12 24 and and CC cord-323428-jd91k19z 12 25 6 6 CD cord-323428-jd91k19z 12 26 from from IN cord-323428-jd91k19z 12 27 the the DT cord-323428-jd91k19z 12 28 onset onset NN cord-323428-jd91k19z 12 29 of of IN cord-323428-jd91k19z 12 30 the the DT cord-323428-jd91k19z 12 31 first first JJ cord-323428-jd91k19z 12 32 immunization immunization NN cord-323428-jd91k19z 12 33 . . . cord-323428-jd91k19z 13 1 There there EX cord-323428-jd91k19z 13 2 was be VBD cord-323428-jd91k19z 13 3 a a DT cord-323428-jd91k19z 13 4 significant significant JJ cord-323428-jd91k19z 13 5 increase increase NN cord-323428-jd91k19z 13 6 in in IN cord-323428-jd91k19z 13 7 the the DT cord-323428-jd91k19z 13 8 amount amount NN cord-323428-jd91k19z 13 9 of of IN cord-323428-jd91k19z 13 10 Th1-related Th1-related NNP cord-323428-jd91k19z 13 11 cytokines cytokine NNS cord-323428-jd91k19z 13 12 ( ( -LRB- cord-323428-jd91k19z 13 13 interferon interferon NN cord-323428-jd91k19z 13 14 - - HYPH cord-323428-jd91k19z 13 15 γ γ NN cord-323428-jd91k19z 13 16 and and CC cord-323428-jd91k19z 13 17 interleukin interleukin VBN cord-323428-jd91k19z 13 18 [ [ -LRB- cord-323428-jd91k19z 13 19 IL IL NNP cord-323428-jd91k19z 13 20 ] ] -RRB- cord-323428-jd91k19z 13 21 12 12 CD cord-323428-jd91k19z 13 22 ) ) -RRB- cord-323428-jd91k19z 13 23 , , , cord-323428-jd91k19z 13 24 and and CC cord-323428-jd91k19z 13 25 a a DT cord-323428-jd91k19z 13 26 significant significant JJ cord-323428-jd91k19z 13 27 decrease decrease NN cord-323428-jd91k19z 13 28 in in IN cord-323428-jd91k19z 13 29 the the DT cord-323428-jd91k19z 13 30 Th2-related Th2-related NNP cord-323428-jd91k19z 13 31 cytokine cytokine NN cord-323428-jd91k19z 13 32 IL-4 IL-4 NNP cord-323428-jd91k19z 13 33 in in IN cord-323428-jd91k19z 13 34 splenocyte splenocyte NNP cord-323428-jd91k19z 13 35 cell cell NN cord-323428-jd91k19z 13 36 culture culture NN cord-323428-jd91k19z 13 37 of of IN cord-323428-jd91k19z 13 38 the the DT cord-323428-jd91k19z 13 39 vaccinated vaccinated JJ cord-323428-jd91k19z 13 40 group group NN cord-323428-jd91k19z 13 41 compared compare VBN cord-323428-jd91k19z 13 42 with with IN cord-323428-jd91k19z 13 43 the the DT cord-323428-jd91k19z 13 44 control control NN cord-323428-jd91k19z 13 45 groups group NNS cord-323428-jd91k19z 13 46 . . . cord-323428-jd91k19z 14 1 CONCLUSION conclusion NN cord-323428-jd91k19z 14 2 : : : cord-323428-jd91k19z 15 1 The the DT cord-323428-jd91k19z 15 2 results result NNS cord-323428-jd91k19z 15 3 of of IN cord-323428-jd91k19z 15 4 this this DT cord-323428-jd91k19z 15 5 study study NN cord-323428-jd91k19z 15 6 suggest suggest VBP cord-323428-jd91k19z 15 7 that that IN cord-323428-jd91k19z 15 8 this this DT cord-323428-jd91k19z 15 9 recombinant recombinant JJ cord-323428-jd91k19z 15 10 adenovirus adenovirus NN cord-323428-jd91k19z 15 11 vaccine vaccine NN cord-323428-jd91k19z 15 12 encoding encode VBG cord-323428-jd91k19z 15 13 the the DT cord-323428-jd91k19z 15 14 S1 S1 NNP cord-323428-jd91k19z 15 15 subunit subunit NN cord-323428-jd91k19z 15 16 of of IN cord-323428-jd91k19z 15 17 MERS MERS NNP cord-323428-jd91k19z 15 18 - - HYPH cord-323428-jd91k19z 15 19 CoV CoV NNP cord-323428-jd91k19z 15 20 elicits elicit VBZ cord-323428-jd91k19z 15 21 potentially potentially RB cord-323428-jd91k19z 15 22 protective protective JJ cord-323428-jd91k19z 15 23 antigen antigen NN cord-323428-jd91k19z 15 24 - - HYPH cord-323428-jd91k19z 15 25 specific specific JJ cord-323428-jd91k19z 15 26 humoral humoral JJ cord-323428-jd91k19z 15 27 and and CC cord-323428-jd91k19z 15 28 cellular cellular JJ cord-323428-jd91k19z 15 29 immune immune JJ cord-323428-jd91k19z 15 30 responses response NNS cord-323428-jd91k19z 15 31 in in IN cord-323428-jd91k19z 15 32 mice mouse NNS cord-323428-jd91k19z 15 33 . . . cord-323428-jd91k19z 16 1 This this DT cord-323428-jd91k19z 16 2 study study NN cord-323428-jd91k19z 16 3 demonstrates demonstrate VBZ cord-323428-jd91k19z 16 4 a a DT cord-323428-jd91k19z 16 5 promising promising JJ cord-323428-jd91k19z 16 6 vaccine vaccine NN cord-323428-jd91k19z 16 7 for for IN cord-323428-jd91k19z 16 8 the the DT cord-323428-jd91k19z 16 9 control control NN cord-323428-jd91k19z 16 10 and/or and/or CC cord-323428-jd91k19z 16 11 prevention prevention NN cord-323428-jd91k19z 16 12 of of IN cord-323428-jd91k19z 16 13 MERS MERS NNP cord-323428-jd91k19z 16 14 - - HYPH cord-323428-jd91k19z 16 15 CoV cov NN cord-323428-jd91k19z 16 16 infection infection NN cord-323428-jd91k19z 16 17 in in IN cord-323428-jd91k19z 16 18 humans human NNS cord-323428-jd91k19z 16 19 . . . cord-323428-jd91k19z 17 1 Middle Middle NNP cord-323428-jd91k19z 17 2 East East NNP cord-323428-jd91k19z 17 3 respiratory respiratory JJ cord-323428-jd91k19z 17 4 syndrome syndrome NN cord-323428-jd91k19z 17 5 coronavirus coronavirus NN cord-323428-jd91k19z 17 6 ( ( -LRB- cord-323428-jd91k19z 17 7 MERS MERS NNP cord-323428-jd91k19z 17 8 - - HYPH cord-323428-jd91k19z 17 9 CoV CoV NNP cord-323428-jd91k19z 17 10 ) ) -RRB- cord-323428-jd91k19z 17 11 is be VBZ cord-323428-jd91k19z 17 12 a a DT cord-323428-jd91k19z 17 13 newly newly RB cord-323428-jd91k19z 17 14 emerging emerge VBG cord-323428-jd91k19z 17 15 human human JJ cord-323428-jd91k19z 17 16 coronavirus coronavirus NN cord-323428-jd91k19z 17 17 that that WDT cord-323428-jd91k19z 17 18 was be VBD cord-323428-jd91k19z 17 19 discovered discover VBN cord-323428-jd91k19z 17 20 in in IN cord-323428-jd91k19z 17 21 2012 2012 CD cord-323428-jd91k19z 17 22 in in IN cord-323428-jd91k19z 17 23 a a DT cord-323428-jd91k19z 17 24 60-year 60-year JJ cord-323428-jd91k19z 17 25 - - HYPH cord-323428-jd91k19z 17 26 old old JJ cord-323428-jd91k19z 17 27 Saudi saudi JJ cord-323428-jd91k19z 17 28 Arabian arabian JJ cord-323428-jd91k19z 17 29 man man NN cord-323428-jd91k19z 17 30 [ [ -LRB- cord-323428-jd91k19z 17 31 1 1 CD cord-323428-jd91k19z 17 32 ] ] -RRB- cord-323428-jd91k19z 17 33 . . . cord-323428-jd91k19z 18 1 Following follow VBG cord-323428-jd91k19z 18 2 its -PRON- PRP$ cord-323428-jd91k19z 18 3 discovery discovery NN cord-323428-jd91k19z 18 4 , , , cord-323428-jd91k19z 18 5 many many JJ cord-323428-jd91k19z 18 6 cases case NNS cord-323428-jd91k19z 18 7 were be VBD cord-323428-jd91k19z 18 8 identified identify VBN cord-323428-jd91k19z 18 9 in in IN cord-323428-jd91k19z 18 10 different different JJ cord-323428-jd91k19z 18 11 regions region NNS cord-323428-jd91k19z 18 12 of of IN cord-323428-jd91k19z 18 13 the the DT cord-323428-jd91k19z 18 14 Arabian Arabian NNP cord-323428-jd91k19z 18 15 Peninsula Peninsula NNP cord-323428-jd91k19z 18 16 and and CC cord-323428-jd91k19z 18 17 worldwide worldwide RB cord-323428-jd91k19z 18 18 thereafter thereafter RB cord-323428-jd91k19z 18 19 [ [ -LRB- cord-323428-jd91k19z 18 20 2 2 CD cord-323428-jd91k19z 18 21 , , , cord-323428-jd91k19z 18 22 3 3 CD cord-323428-jd91k19z 18 23 ] ] -RRB- cord-323428-jd91k19z 18 24 . . . cord-323428-jd91k19z 19 1 The the DT cord-323428-jd91k19z 19 2 most most RBS cord-323428-jd91k19z 19 3 recent recent JJ cord-323428-jd91k19z 19 4 outbreak outbreak NN cord-323428-jd91k19z 19 5 occurred occur VBD cord-323428-jd91k19z 19 6 in in IN cord-323428-jd91k19z 19 7 June June NNP cord-323428-jd91k19z 19 8 2015 2015 CD cord-323428-jd91k19z 19 9 in in IN cord-323428-jd91k19z 19 10 South South NNP cord-323428-jd91k19z 19 11 Korea Korea NNP cord-323428-jd91k19z 19 12 and and CC cord-323428-jd91k19z 19 13 was be VBD cord-323428-jd91k19z 19 14 linked link VBN cord-323428-jd91k19z 19 15 to to IN cord-323428-jd91k19z 19 16 a a DT cord-323428-jd91k19z 19 17 South south JJ cord-323428-jd91k19z 19 18 Korean korean JJ cord-323428-jd91k19z 19 19 man man NN cord-323428-jd91k19z 19 20 who who WP cord-323428-jd91k19z 19 21 had have VBD cord-323428-jd91k19z 19 22 recently recently RB cord-323428-jd91k19z 19 23 traveled travel VBN cord-323428-jd91k19z 19 24 to to IN cord-323428-jd91k19z 19 25 the the DT cord-323428-jd91k19z 19 26 Middle Middle NNP cord-323428-jd91k19z 19 27 East East NNP cord-323428-jd91k19z 19 28 [ [ -LRB- cord-323428-jd91k19z 19 29 3 3 CD cord-323428-jd91k19z 19 30 ] ] -RRB- cord-323428-jd91k19z 19 31 . . . cord-323428-jd91k19z 20 1 The the DT cord-323428-jd91k19z 20 2 infection infection NN cord-323428-jd91k19z 20 3 then then RB cord-323428-jd91k19z 20 4 rapidly rapidly RB cord-323428-jd91k19z 20 5 spread spread VBD cord-323428-jd91k19z 20 6 to to IN cord-323428-jd91k19z 20 7 26 26 CD cord-323428-jd91k19z 20 8 persons person NNS cord-323428-jd91k19z 20 9 through through IN cord-323428-jd91k19z 20 10 close close JJ cord-323428-jd91k19z 20 11 contact contact NN cord-323428-jd91k19z 20 12 in in IN cord-323428-jd91k19z 20 13 a a DT cord-323428-jd91k19z 20 14 hospital hospital NN cord-323428-jd91k19z 20 15 . . . cord-323428-jd91k19z 21 1 Within within IN cord-323428-jd91k19z 21 2 a a DT cord-323428-jd91k19z 21 3 few few JJ cord-323428-jd91k19z 21 4 months month NNS cord-323428-jd91k19z 21 5 , , , cord-323428-jd91k19z 21 6 many many JJ cord-323428-jd91k19z 21 7 cases case NNS cord-323428-jd91k19z 21 8 ( ( -LRB- cord-323428-jd91k19z 21 9 n=186 n=186 CD cord-323428-jd91k19z 21 10 ) ) -RRB- cord-323428-jd91k19z 21 11 were be VBD cord-323428-jd91k19z 21 12 reported report VBN cord-323428-jd91k19z 21 13 in in IN cord-323428-jd91k19z 21 14 hospitalized hospitalize VBN cord-323428-jd91k19z 21 15 and and CC cord-323428-jd91k19z 21 16 non non JJ cord-323428-jd91k19z 21 17 - - JJ cord-323428-jd91k19z 21 18 hospitalized hospitalize VBN cord-323428-jd91k19z 21 19 persons person NNS cord-323428-jd91k19z 21 20 in in IN cord-323428-jd91k19z 21 21 South South NNP cord-323428-jd91k19z 21 22 Korea Korea NNP cord-323428-jd91k19z 21 23 [ [ -LRB- cord-323428-jd91k19z 21 24 3 3 CD cord-323428-jd91k19z 21 25 ] ] -RRB- cord-323428-jd91k19z 21 26 . . . cord-323428-jd91k19z 22 1 The the DT cord-323428-jd91k19z 22 2 disease disease NN cord-323428-jd91k19z 22 3 showed show VBD cord-323428-jd91k19z 22 4 a a DT cord-323428-jd91k19z 22 5 high high JJ cord-323428-jd91k19z 22 6 mortality mortality NN cord-323428-jd91k19z 22 7 rate rate NN cord-323428-jd91k19z 22 8 that that WDT cord-323428-jd91k19z 22 9 reached reach VBD cord-323428-jd91k19z 22 10 up up RP cord-323428-jd91k19z 22 11 to to TO cord-323428-jd91k19z 22 12 40 40 CD cord-323428-jd91k19z 22 13 % % NN cord-323428-jd91k19z 22 14 , , , cord-323428-jd91k19z 22 15 which which WDT cord-323428-jd91k19z 22 16 was be VBD cord-323428-jd91k19z 22 17 higher high JJR cord-323428-jd91k19z 22 18 than than IN cord-323428-jd91k19z 22 19 that that DT cord-323428-jd91k19z 22 20 of of IN cord-323428-jd91k19z 22 21 the the DT cord-323428-jd91k19z 22 22 severe severe JJ cord-323428-jd91k19z 22 23 acute acute JJ cord-323428-jd91k19z 22 24 respiratory respiratory JJ cord-323428-jd91k19z 22 25 syndrome syndrome NN cord-323428-jd91k19z 22 26 coronavirus coronavirus NN cord-323428-jd91k19z 22 27 ( ( -LRB- cord-323428-jd91k19z 22 28 SARS SARS NNP cord-323428-jd91k19z 22 29 - - HYPH cord-323428-jd91k19z 22 30 CoV CoV NNP cord-323428-jd91k19z 22 31 ) ) -RRB- cord-323428-jd91k19z 22 32 outbreak outbreak NN cord-323428-jd91k19z 22 33 in in IN cord-323428-jd91k19z 22 34 2002 2002 CD cord-323428-jd91k19z 22 35 - - SYM cord-323428-jd91k19z 22 36 2003 2003 CD cord-323428-jd91k19z 22 37 ( ( -LRB- cord-323428-jd91k19z 22 38 10 10 CD cord-323428-jd91k19z 22 39 % % NN cord-323428-jd91k19z 22 40 ) ) -RRB- cord-323428-jd91k19z 22 41 [ [ -LRB- cord-323428-jd91k19z 22 42 4 4 CD cord-323428-jd91k19z 22 43 ] ] -RRB- cord-323428-jd91k19z 22 44 . . . cord-323428-jd91k19z 23 1 Coronaviruses coronaviruse NNS cord-323428-jd91k19z 23 2 belong belong VBP cord-323428-jd91k19z 23 3 to to IN cord-323428-jd91k19z 23 4 the the DT cord-323428-jd91k19z 23 5 subfamily subfamily NN cord-323428-jd91k19z 23 6 Coronavirinae Coronavirinae NNP cord-323428-jd91k19z 23 7 within within IN cord-323428-jd91k19z 23 8 the the DT cord-323428-jd91k19z 23 9 family family NN cord-323428-jd91k19z 23 10 Coronaviridae Coronaviridae NNP cord-323428-jd91k19z 23 11 of of IN cord-323428-jd91k19z 23 12 the the DT cord-323428-jd91k19z 23 13 order order NN cord-323428-jd91k19z 24 1 Nidovirales Nidovirales NNP cord-323428-jd91k19z 24 2 [ [ -LRB- cord-323428-jd91k19z 24 3 5 5 CD cord-323428-jd91k19z 24 4 ] ] -RRB- cord-323428-jd91k19z 24 5 . . . cord-323428-jd91k19z 25 1 Five five CD cord-323428-jd91k19z 25 2 human human JJ cord-323428-jd91k19z 25 3 coronaviruses coronaviruse NNS cord-323428-jd91k19z 25 4 were be VBD cord-323428-jd91k19z 25 5 identified identify VBN cord-323428-jd91k19z 25 6 ( ( -LRB- cord-323428-jd91k19z 25 7 229E 229e CD cord-323428-jd91k19z 25 8 , , , cord-323428-jd91k19z 25 9 OC43 OC43 NNP cord-323428-jd91k19z 25 10 , , , cord-323428-jd91k19z 25 11 NL63 NL63 NNP cord-323428-jd91k19z 25 12 , , , cord-323428-jd91k19z 25 13 HKU1 HKU1 NNP cord-323428-jd91k19z 25 14 , , , cord-323428-jd91k19z 25 15 and and CC cord-323428-jd91k19z 25 16 SARS SARS NNP cord-323428-jd91k19z 25 17 - - HYPH cord-323428-jd91k19z 25 18 CoV CoV NNP cord-323428-jd91k19z 25 19 ) ) -RRB- cord-323428-jd91k19z 25 20 before before IN cord-323428-jd91k19z 25 21 MERS MERS NNP cord-323428-jd91k19z 25 22 - - HYPH cord-323428-jd91k19z 25 23 CoV. CoV. NNP cord-323428-jd91k19z 25 24 Lineage Lineage NNP cord-323428-jd91k19z 25 25 C C NNP cord-323428-jd91k19z 25 26 of of IN cord-323428-jd91k19z 25 27 betacoronaviruses betacoronaviruses NNP cord-323428-jd91k19z 25 28 includes include VBZ cord-323428-jd91k19z 25 29 bat bat NN cord-323428-jd91k19z 25 30 coronaviruses coronaviruse NNS cord-323428-jd91k19z 25 31 , , , cord-323428-jd91k19z 25 32 which which WDT cord-323428-jd91k19z 25 33 give give VBP cord-323428-jd91k19z 25 34 a a DT cord-323428-jd91k19z 25 35 primitive primitive JJ cord-323428-jd91k19z 25 36 impression impression NN cord-323428-jd91k19z 25 37 regarding regard VBG cord-323428-jd91k19z 25 38 the the DT cord-323428-jd91k19z 25 39 origin origin NN cord-323428-jd91k19z 25 40 of of IN cord-323428-jd91k19z 25 41 the the DT cord-323428-jd91k19z 25 42 virus virus NN cord-323428-jd91k19z 25 43 [ [ -LRB- cord-323428-jd91k19z 25 44 6 6 CD cord-323428-jd91k19z 25 45 ] ] -RRB- cord-323428-jd91k19z 25 46 . . . cord-323428-jd91k19z 26 1 The the DT cord-323428-jd91k19z 26 2 detection detection NN cord-323428-jd91k19z 26 3 of of IN cord-323428-jd91k19z 26 4 MERS MERS NNP cord-323428-jd91k19z 26 5 - - HYPH cord-323428-jd91k19z 26 6 CoV CoV NNP cord-323428-jd91k19z 26 7 and and CC cord-323428-jd91k19z 26 8 its -PRON- PRP$ cord-323428-jd91k19z 26 9 neutralizing neutralize VBG cord-323428-jd91k19z 26 10 antibodies antibody NNS cord-323428-jd91k19z 26 11 in in IN cord-323428-jd91k19z 26 12 the the DT cord-323428-jd91k19z 26 13 sera sera NNP cord-323428-jd91k19z 26 14 of of IN cord-323428-jd91k19z 26 15 dromedary dromedary NN cord-323428-jd91k19z 26 16 camels camel NNS cord-323428-jd91k19z 26 17 has have VBZ cord-323428-jd91k19z 26 18 shed shed VBN cord-323428-jd91k19z 26 19 light light NN cord-323428-jd91k19z 26 20 on on IN cord-323428-jd91k19z 26 21 the the DT cord-323428-jd91k19z 26 22 role role NN cord-323428-jd91k19z 26 23 of of IN cord-323428-jd91k19z 26 24 the the DT cord-323428-jd91k19z 26 25 camel camel NN cord-323428-jd91k19z 26 26 as as IN cord-323428-jd91k19z 26 27 a a DT cord-323428-jd91k19z 26 28 possible possible JJ cord-323428-jd91k19z 26 29 animal animal NN cord-323428-jd91k19z 26 30 reservoir reservoir NN cord-323428-jd91k19z 26 31 , , , cord-323428-jd91k19z 26 32 which which WDT cord-323428-jd91k19z 26 33 may may MD cord-323428-jd91k19z 26 34 transmit transmit VB cord-323428-jd91k19z 26 35 the the DT cord-323428-jd91k19z 26 36 virus virus NN cord-323428-jd91k19z 26 37 to to IN cord-323428-jd91k19z 26 38 humans human NNS cord-323428-jd91k19z 26 39 [ [ -LRB- cord-323428-jd91k19z 26 40 7 7 CD cord-323428-jd91k19z 26 41 ] ] -RRB- cord-323428-jd91k19z 26 42 [ [ -LRB- cord-323428-jd91k19z 26 43 8 8 CD cord-323428-jd91k19z 26 44 ] ] -RRB- cord-323428-jd91k19z 26 45 [ [ -LRB- cord-323428-jd91k19z 26 46 9 9 CD cord-323428-jd91k19z 26 47 ] ] -RRB- cord-323428-jd91k19z 26 48 [ [ -LRB- cord-323428-jd91k19z 26 49 10 10 CD cord-323428-jd91k19z 26 50 ] ] -RRB- cord-323428-jd91k19z 26 51 . . . cord-323428-jd91k19z 27 1 Indeed indeed RB cord-323428-jd91k19z 27 2 , , , cord-323428-jd91k19z 27 3 researchers researcher NNS cord-323428-jd91k19z 27 4 isolated isolate VBD cord-323428-jd91k19z 27 5 the the DT cord-323428-jd91k19z 27 6 same same JJ cord-323428-jd91k19z 27 7 MERS MERS NNP cord-323428-jd91k19z 27 8 - - HYPH cord-323428-jd91k19z 27 9 CoV cov NN cord-323428-jd91k19z 27 10 strain strain NN cord-323428-jd91k19z 27 11 from from IN cord-323428-jd91k19z 27 12 both both CC cord-323428-jd91k19z 27 13 a a DT cord-323428-jd91k19z 27 14 camel camel NN cord-323428-jd91k19z 27 15 " " '' cord-323428-jd91k19z 27 16 in in IN cord-323428-jd91k19z 27 17 a a DT cord-323428-jd91k19z 27 18 barn barn NN cord-323428-jd91k19z 27 19 " " '' cord-323428-jd91k19z 27 20 and and CC cord-323428-jd91k19z 27 21 its -PRON- PRP$ cord-323428-jd91k19z 27 22 infected infected JJ cord-323428-jd91k19z 27 23 owner owner NN cord-323428-jd91k19z 27 24 in in IN cord-323428-jd91k19z 27 25 Saudi Saudi NNP cord-323428-jd91k19z 27 26 Arabia Arabia NNP cord-323428-jd91k19z 27 27 , , , cord-323428-jd91k19z 27 28 thus thus RB cord-323428-jd91k19z 27 29 providing provide VBG cord-323428-jd91k19z 27 30 further further JJ cord-323428-jd91k19z 27 31 evidence evidence NN cord-323428-jd91k19z 27 32 of of IN cord-323428-jd91k19z 27 33 the the DT cord-323428-jd91k19z 27 34 potential potential JJ cord-323428-jd91k19z 27 35 airborne airborne JJ cord-323428-jd91k19z 27 36 and and CC cord-323428-jd91k19z 27 37 direct direct JJ cord-323428-jd91k19z 27 38 contact contact NN cord-323428-jd91k19z 27 39 transmission transmission NN cord-323428-jd91k19z 27 40 of of IN cord-323428-jd91k19z 27 41 the the DT cord-323428-jd91k19z 27 42 virus virus NN cord-323428-jd91k19z 27 43 between between IN cord-323428-jd91k19z 27 44 camels camel NNS cord-323428-jd91k19z 27 45 and and CC cord-323428-jd91k19z 27 46 humans human NNS cord-323428-jd91k19z 27 47 [ [ -LRB- cord-323428-jd91k19z 27 48 11 11 CD cord-323428-jd91k19z 27 49 ] ] -RRB- cord-323428-jd91k19z 27 50 . . . cord-323428-jd91k19z 28 1 There there EX cord-323428-jd91k19z 28 2 have have VBP cord-323428-jd91k19z 28 3 been be VBN cord-323428-jd91k19z 28 4 several several JJ cord-323428-jd91k19z 28 5 attempts attempt NNS cord-323428-jd91k19z 28 6 to to TO cord-323428-jd91k19z 28 7 develop develop VB cord-323428-jd91k19z 28 8 a a DT cord-323428-jd91k19z 28 9 protective protective JJ cord-323428-jd91k19z 28 10 vaccine vaccine NN cord-323428-jd91k19z 28 11 against against IN cord-323428-jd91k19z 28 12 MERS MERS NNP cord-323428-jd91k19z 28 13 - - HYPH cord-323428-jd91k19z 28 14 CoV CoV NNP cord-323428-jd91k19z 28 15 [ [ -LRB- cord-323428-jd91k19z 28 16 12 12 CD cord-323428-jd91k19z 28 17 ] ] -RRB- cord-323428-jd91k19z 28 18 [ [ -LRB- cord-323428-jd91k19z 28 19 13 13 CD cord-323428-jd91k19z 28 20 ] ] -RRB- cord-323428-jd91k19z 28 21 [ [ -LRB- cord-323428-jd91k19z 28 22 14 14 CD cord-323428-jd91k19z 28 23 ] ] -RRB- cord-323428-jd91k19z 28 24 [ [ -LRB- cord-323428-jd91k19z 28 25 15 15 CD cord-323428-jd91k19z 28 26 ] ] -RRB- cord-323428-jd91k19z 28 27 [ [ -LRB- cord-323428-jd91k19z 28 28 16 16 CD cord-323428-jd91k19z 28 29 ] ] -RRB- cord-323428-jd91k19z 28 30 [ [ -LRB- cord-323428-jd91k19z 28 31 17 17 CD cord-323428-jd91k19z 28 32 ] ] -RRB- cord-323428-jd91k19z 28 33 [ [ -LRB- cord-323428-jd91k19z 28 34 18 18 CD cord-323428-jd91k19z 28 35 ] ] -RRB- cord-323428-jd91k19z 28 36 [ [ -LRB- cord-323428-jd91k19z 28 37 19 19 CD cord-323428-jd91k19z 28 38 ] ] -RRB- cord-323428-jd91k19z 28 39 [ [ -LRB- cord-323428-jd91k19z 28 40 20 20 CD cord-323428-jd91k19z 28 41 ] ] -RRB- cord-323428-jd91k19z 28 42 [ [ -LRB- cord-323428-jd91k19z 28 43 21 21 CD cord-323428-jd91k19z 28 44 ] ] -RRB- cord-323428-jd91k19z 28 45 [ [ -LRB- cord-323428-jd91k19z 28 46 22 22 CD cord-323428-jd91k19z 28 47 ] ] -RRB- cord-323428-jd91k19z 28 48 [ [ -LRB- cord-323428-jd91k19z 28 49 23 23 CD cord-323428-jd91k19z 28 50 ] ] -RRB- cord-323428-jd91k19z 28 51 . . . cord-323428-jd91k19z 29 1 Researchers researcher NNS cord-323428-jd91k19z 29 2 around around IN cord-323428-jd91k19z 29 3 the the DT cord-323428-jd91k19z 29 4 world world NN cord-323428-jd91k19z 29 5 are be VBP cord-323428-jd91k19z 29 6 focused focus VBN cord-323428-jd91k19z 29 7 on on IN cord-323428-jd91k19z 29 8 the the DT cord-323428-jd91k19z 29 9 spike spike NN cord-323428-jd91k19z 29 10 protein protein NN cord-323428-jd91k19z 29 11 as as IN cord-323428-jd91k19z 29 12 the the DT cord-323428-jd91k19z 29 13 main main JJ cord-323428-jd91k19z 29 14 target target NN cord-323428-jd91k19z 29 15 for for IN cord-323428-jd91k19z 29 16 vaccine vaccine NN cord-323428-jd91k19z 29 17 development development NN cord-323428-jd91k19z 29 18 against against IN cord-323428-jd91k19z 29 19 MERS MERS NNP cord-323428-jd91k19z 29 20 - - HYPH cord-323428-jd91k19z 29 21 CoV. CoV. NNP cord-323428-jd91k19z 30 1 The the DT cord-323428-jd91k19z 30 2 spike spike NN cord-323428-jd91k19z 30 3 protein protein NN cord-323428-jd91k19z 30 4 of of IN cord-323428-jd91k19z 30 5 MERS MERS NNP cord-323428-jd91k19z 30 6 - - HYPH cord-323428-jd91k19z 30 7 CoV CoV NNP cord-323428-jd91k19z 30 8 attaches attach VBZ cord-323428-jd91k19z 30 9 to to IN cord-323428-jd91k19z 30 10 the the DT cord-323428-jd91k19z 30 11 host host NN cord-323428-jd91k19z 30 12 dipeptidyl dipeptidyl NN cord-323428-jd91k19z 30 13 peptidase-4 peptidase-4 NNP cord-323428-jd91k19z 30 14 ( ( -LRB- cord-323428-jd91k19z 30 15 DPP4 DPP4 NNP cord-323428-jd91k19z 30 16 ) ) -RRB- cord-323428-jd91k19z 30 17 receptor receptor NN cord-323428-jd91k19z 30 18 , , , cord-323428-jd91k19z 30 19 which which WDT cord-323428-jd91k19z 30 20 is be VBZ cord-323428-jd91k19z 30 21 expressed express VBN cord-323428-jd91k19z 30 22 on on IN cord-323428-jd91k19z 30 23 several several JJ cord-323428-jd91k19z 30 24 types type NNS cord-323428-jd91k19z 30 25 of of IN cord-323428-jd91k19z 30 26 human human JJ cord-323428-jd91k19z 30 27 cells cell NNS cord-323428-jd91k19z 30 28 [ [ -LRB- cord-323428-jd91k19z 30 29 24 24 CD cord-323428-jd91k19z 30 30 ] ] -RRB- cord-323428-jd91k19z 30 31 . . . cord-323428-jd91k19z 31 1 Many many JJ cord-323428-jd91k19z 31 2 studies study NNS cord-323428-jd91k19z 31 3 published publish VBN cord-323428-jd91k19z 31 4 since since IN cord-323428-jd91k19z 31 5 2012 2012 CD cord-323428-jd91k19z 31 6 suggesting suggest VBG cord-323428-jd91k19z 31 7 vaccine vaccine NN cord-323428-jd91k19z 31 8 models model NNS cord-323428-jd91k19z 31 9 were be VBD cord-323428-jd91k19z 31 10 constructed construct VBN cord-323428-jd91k19z 31 11 based base VBN cord-323428-jd91k19z 31 12 on on IN cord-323428-jd91k19z 31 13 the the DT cord-323428-jd91k19z 31 14 spike spike NN cord-323428-jd91k19z 31 15 protein protein NN cord-323428-jd91k19z 31 16 and and CC cord-323428-jd91k19z 31 17 its -PRON- PRP$ cord-323428-jd91k19z 31 18 receptor receptor NN cord-323428-jd91k19z 31 19 - - HYPH cord-323428-jd91k19z 31 20 binding bind VBG cord-323428-jd91k19z 31 21 domain domain NN cord-323428-jd91k19z 31 22 ( ( -LRB- cord-323428-jd91k19z 31 23 RBD RBD NNP cord-323428-jd91k19z 31 24 ) ) -RRB- cord-323428-jd91k19z 31 25 region region NN cord-323428-jd91k19z 31 26 to to TO cord-323428-jd91k19z 31 27 elicit elicit VB cord-323428-jd91k19z 31 28 a a DT cord-323428-jd91k19z 31 29 strong strong JJ cord-323428-jd91k19z 31 30 and and CC cord-323428-jd91k19z 31 31 protective protective JJ cord-323428-jd91k19z 31 32 immune immune JJ cord-323428-jd91k19z 31 33 response response NN cord-323428-jd91k19z 31 34 in in IN cord-323428-jd91k19z 31 35 vitro vitro FW cord-323428-jd91k19z 31 36 and and CC cord-323428-jd91k19z 31 37 in in IN cord-323428-jd91k19z 31 38 vivo vivo NNP cord-323428-jd91k19z 31 39 [ [ -LRB- cord-323428-jd91k19z 31 40 25 25 CD cord-323428-jd91k19z 31 41 ] ] -RRB- cord-323428-jd91k19z 31 42 . . . cord-323428-jd91k19z 32 1 Recombinant recombinant JJ cord-323428-jd91k19z 32 2 adenoviral adenoviral JJ cord-323428-jd91k19z 32 3 vector vector NN cord-323428-jd91k19z 32 4 vaccines vaccine NNS cord-323428-jd91k19z 32 5 are be VBP cord-323428-jd91k19z 32 6 one one CD cord-323428-jd91k19z 32 7 of of IN cord-323428-jd91k19z 32 8 the the DT cord-323428-jd91k19z 32 9 most most RBS cord-323428-jd91k19z 32 10 effective effective JJ cord-323428-jd91k19z 32 11 vaccines vaccine NNS cord-323428-jd91k19z 32 12 and and CC cord-323428-jd91k19z 32 13 showed show VBD cord-323428-jd91k19z 32 14 interesting interesting JJ cord-323428-jd91k19z 32 15 results result NNS cord-323428-jd91k19z 32 16 during during IN cord-323428-jd91k19z 32 17 SARS SARS NNP cord-323428-jd91k19z 32 18 - - HYPH cord-323428-jd91k19z 32 19 CoV CoV NNP cord-323428-jd91k19z 32 20 outbreaks outbreak NNS cord-323428-jd91k19z 32 21 [ [ -LRB- cord-323428-jd91k19z 32 22 12 12 CD cord-323428-jd91k19z 32 23 , , , cord-323428-jd91k19z 32 24 26 26 CD cord-323428-jd91k19z 32 25 , , , cord-323428-jd91k19z 32 26 27 27 CD cord-323428-jd91k19z 32 27 ] ] -RRB- cord-323428-jd91k19z 32 28 . . . cord-323428-jd91k19z 33 1 Since since IN cord-323428-jd91k19z 33 2 2013 2013 CD cord-323428-jd91k19z 33 3 , , , cord-323428-jd91k19z 33 4 several several JJ cord-323428-jd91k19z 33 5 studies study NNS cord-323428-jd91k19z 33 6 were be VBD cord-323428-jd91k19z 33 7 published publish VBN cord-323428-jd91k19z 33 8 , , , cord-323428-jd91k19z 33 9 in in IN cord-323428-jd91k19z 33 10 which which WDT cord-323428-jd91k19z 33 11 different different JJ cord-323428-jd91k19z 33 12 viral viral JJ cord-323428-jd91k19z 33 13 vectors vector NNS cord-323428-jd91k19z 33 14 ( ( -LRB- cord-323428-jd91k19z 33 15 e.g. e.g. RB cord-323428-jd91k19z 33 16 , , , cord-323428-jd91k19z 33 17 adenoviruses adenovirus NNS cord-323428-jd91k19z 33 18 and and CC cord-323428-jd91k19z 33 19 vaccinia vaccinia NN cord-323428-jd91k19z 33 20 virus virus NN cord-323428-jd91k19z 33 21 ) ) -RRB- cord-323428-jd91k19z 33 22 were be VBD cord-323428-jd91k19z 33 23 used use VBN cord-323428-jd91k19z 33 24 to to TO cord-323428-jd91k19z 33 25 develop develop VB cord-323428-jd91k19z 33 26 recombinant recombinant JJ cord-323428-jd91k19z 33 27 vaccine vaccine NN cord-323428-jd91k19z 33 28 candidates candidate NNS cord-323428-jd91k19z 33 29 based base VBN cord-323428-jd91k19z 33 30 on on IN cord-323428-jd91k19z 33 31 full full JJ cord-323428-jd91k19z 33 32 spike spike NN cord-323428-jd91k19z 33 33 gene gene NN cord-323428-jd91k19z 33 34 or or CC cord-323428-jd91k19z 33 35 part part NN cord-323428-jd91k19z 33 36 of of IN cord-323428-jd91k19z 33 37 it -PRON- PRP cord-323428-jd91k19z 33 38 and and CC cord-323428-jd91k19z 33 39 tested test VBD cord-323428-jd91k19z 33 40 their -PRON- PRP$ cord-323428-jd91k19z 33 41 ability ability NN cord-323428-jd91k19z 33 42 to to TO cord-323428-jd91k19z 33 43 produce produce VB cord-323428-jd91k19z 33 44 protective protective JJ cord-323428-jd91k19z 33 45 immunity immunity NN cord-323428-jd91k19z 33 46 against against IN cord-323428-jd91k19z 33 47 MERS MERS NNP cord-323428-jd91k19z 33 48 - - HYPH cord-323428-jd91k19z 33 49 CoV CoV NNP cord-323428-jd91k19z 33 50 infection infection NN cord-323428-jd91k19z 33 51 [ [ -LRB- cord-323428-jd91k19z 33 52 13 13 CD cord-323428-jd91k19z 33 53 ] ] -RRB- cord-323428-jd91k19z 34 1 [ [ -LRB- cord-323428-jd91k19z 34 2 14 14 CD cord-323428-jd91k19z 34 3 ] ] -RRB- cord-323428-jd91k19z 35 1 [ [ -LRB- cord-323428-jd91k19z 35 2 15 15 CD cord-323428-jd91k19z 35 3 ] ] -RRB- cord-323428-jd91k19z 35 4 [ [ -LRB- cord-323428-jd91k19z 35 5 16 16 CD cord-323428-jd91k19z 35 6 ] ] -RRB- cord-323428-jd91k19z 35 7 [ [ -LRB- cord-323428-jd91k19z 35 8 17 17 CD cord-323428-jd91k19z 35 9 ] ] -RRB- cord-323428-jd91k19z 35 10 [ [ -LRB- cord-323428-jd91k19z 35 11 18 18 CD cord-323428-jd91k19z 35 12 ] ] -RRB- cord-323428-jd91k19z 35 13 [ [ -LRB- cord-323428-jd91k19z 35 14 19 19 CD cord-323428-jd91k19z 35 15 ] ] -RRB- cord-323428-jd91k19z 36 1 [ [ -LRB- cord-323428-jd91k19z 36 2 20 20 CD cord-323428-jd91k19z 36 3 ] ] -RRB- cord-323428-jd91k19z 36 4 [ [ -LRB- cord-323428-jd91k19z 36 5 21 21 CD cord-323428-jd91k19z 36 6 ] ] -RRB- cord-323428-jd91k19z 37 1 [ [ -LRB- cord-323428-jd91k19z 37 2 22 22 CD cord-323428-jd91k19z 37 3 ] ] -RRB- cord-323428-jd91k19z 37 4 [ [ -LRB- cord-323428-jd91k19z 37 5 23 23 CD cord-323428-jd91k19z 37 6 ] ] -RRB- cord-323428-jd91k19z 37 7 . . . cord-323428-jd91k19z 38 1 However however RB cord-323428-jd91k19z 38 2 , , , cord-323428-jd91k19z 38 3 further further JJ cord-323428-jd91k19z 38 4 investigations investigation NNS cord-323428-jd91k19z 38 5 are be VBP cord-323428-jd91k19z 38 6 needed need VBN cord-323428-jd91k19z 38 7 on on IN cord-323428-jd91k19z 38 8 these these DT cord-323428-jd91k19z 38 9 suggested suggest VBN cord-323428-jd91k19z 38 10 vaccines vaccine NNS cord-323428-jd91k19z 38 11 including include VBG cord-323428-jd91k19z 38 12 testing test VBG cord-323428-jd91k19z 38 13 their -PRON- PRP$ cord-323428-jd91k19z 38 14 ability ability NN cord-323428-jd91k19z 38 15 to to TO cord-323428-jd91k19z 38 16 elicit elicit VB cord-323428-jd91k19z 38 17 neutralizing neutralize VBG cord-323428-jd91k19z 38 18 antibodies antibody NNS cord-323428-jd91k19z 38 19 in in IN cord-323428-jd91k19z 38 20 different different JJ cord-323428-jd91k19z 38 21 animal animal NN cord-323428-jd91k19z 38 22 models model NNS cord-323428-jd91k19z 38 23 , , , cord-323428-jd91k19z 38 24 stimulation stimulation NN cord-323428-jd91k19z 38 25 of of IN cord-323428-jd91k19z 38 26 both both DT cord-323428-jd91k19z 38 27 innate innate JJ cord-323428-jd91k19z 38 28 and and CC cord-323428-jd91k19z 38 29 adaptive adaptive JJ cord-323428-jd91k19z 38 30 immune immune JJ cord-323428-jd91k19z 38 31 responses response NNS cord-323428-jd91k19z 38 32 and and CC cord-323428-jd91k19z 38 33 their -PRON- PRP$ cord-323428-jd91k19z 38 34 corresponding correspond VBG cord-323428-jd91k19z 38 35 cytokine cytokine NN cord-323428-jd91k19z 38 36 and and CC cord-323428-jd91k19z 38 37 chemokine chemokine NN cord-323428-jd91k19z 38 38 profiles profile NNS cord-323428-jd91k19z 38 39 , , , cord-323428-jd91k19z 38 40 distribution distribution NN cord-323428-jd91k19z 38 41 within within IN cord-323428-jd91k19z 38 42 the the DT cord-323428-jd91k19z 38 43 host host NN cord-323428-jd91k19z 38 44 , , , cord-323428-jd91k19z 38 45 and and CC cord-323428-jd91k19z 38 46 their -PRON- PRP$ cord-323428-jd91k19z 38 47 safety safety NN cord-323428-jd91k19z 38 48 and and CC cord-323428-jd91k19z 38 49 duration duration NN cord-323428-jd91k19z 38 50 profiles profile NNS cord-323428-jd91k19z 38 51 . . . cord-323428-jd91k19z 39 1 In in IN cord-323428-jd91k19z 39 2 this this DT cord-323428-jd91k19z 39 3 study study NN cord-323428-jd91k19z 39 4 , , , cord-323428-jd91k19z 39 5 a a DT cord-323428-jd91k19z 39 6 recombinant recombinant JJ cord-323428-jd91k19z 39 7 adenoviral adenoviral NN cord-323428-jd91k19z 39 8 - - HYPH cord-323428-jd91k19z 39 9 based base VBN cord-323428-jd91k19z 39 10 vaccine vaccine NN cord-323428-jd91k19z 39 11 encoding encode VBG cord-323428-jd91k19z 39 12 the the DT cord-323428-jd91k19z 39 13 spike spike NN cord-323428-jd91k19z 39 14 1 1 CD cord-323428-jd91k19z 39 15 ( ( -LRB- cord-323428-jd91k19z 39 16 S1 S1 NNP cord-323428-jd91k19z 39 17 ) ) -RRB- cord-323428-jd91k19z 39 18 subunit subunit NN cord-323428-jd91k19z 39 19 of of IN cord-323428-jd91k19z 39 20 the the DT cord-323428-jd91k19z 39 21 MERS MERS NNP cord-323428-jd91k19z 39 22 - - HYPH cord-323428-jd91k19z 39 23 CoV CoV NNP cord-323428-jd91k19z 39 24 genome genome NN cord-323428-jd91k19z 39 25 was be VBD cord-323428-jd91k19z 39 26 constructed construct VBN cord-323428-jd91k19z 39 27 , , , cord-323428-jd91k19z 39 28 and and CC cord-323428-jd91k19z 39 29 its -PRON- PRP$ cord-323428-jd91k19z 39 30 humoral humoral JJ cord-323428-jd91k19z 39 31 , , , cord-323428-jd91k19z 39 32 and and CC cord-323428-jd91k19z 39 33 cellular cellular JJ cord-323428-jd91k19z 39 34 immune immune JJ cord-323428-jd91k19z 39 35 responses response NNS cord-323428-jd91k19z 39 36 were be VBD cord-323428-jd91k19z 39 37 evaluated evaluate VBN cord-323428-jd91k19z 39 38 in in IN cord-323428-jd91k19z 39 39 mice mouse NNS cord-323428-jd91k19z 39 40 . . . cord-323428-jd91k19z 40 1 All all DT cord-323428-jd91k19z 40 2 procedures procedure NNS cord-323428-jd91k19z 40 3 performed perform VBN cord-323428-jd91k19z 40 4 in in IN cord-323428-jd91k19z 40 5 this this DT cord-323428-jd91k19z 40 6 study study NN cord-323428-jd91k19z 40 7 were be VBD cord-323428-jd91k19z 40 8 approved approve VBN cord-323428-jd91k19z 40 9 by by IN cord-323428-jd91k19z 40 10 the the DT cord-323428-jd91k19z 40 11 Jordan Jordan NNP cord-323428-jd91k19z 40 12 University University NNP cord-323428-jd91k19z 40 13 of of IN cord-323428-jd91k19z 40 14 Science Science NNP cord-323428-jd91k19z 40 15 and and CC cord-323428-jd91k19z 40 16 Technology Technology NNP cord-323428-jd91k19z 40 17 Animal Animal NNP cord-323428-jd91k19z 40 18 Use Use NNP cord-323428-jd91k19z 40 19 and and CC cord-323428-jd91k19z 40 20 Care Care NNP cord-323428-jd91k19z 40 21 Committee Committee NNP cord-323428-jd91k19z 40 22 . . . cord-323428-jd91k19z 41 1 The the DT cord-323428-jd91k19z 41 2 recombinant recombinant JJ cord-323428-jd91k19z 41 3 human human JJ cord-323428-jd91k19z 41 4 adenovirus adenovirus NN cord-323428-jd91k19z 41 5 type type NN cord-323428-jd91k19z 41 6 5 5 CD cord-323428-jd91k19z 41 7 ( ( -LRB- cord-323428-jd91k19z 41 8 dE1 dE1 NNP cord-323428-jd91k19z 41 9 / / SYM cord-323428-jd91k19z 41 10 E3 E3 NNP cord-323428-jd91k19z 41 11 ) ) -RRB- cord-323428-jd91k19z 41 12 vaccine vaccine NN cord-323428-jd91k19z 41 13 was be VBD cord-323428-jd91k19z 41 14 designed design VBN cord-323428-jd91k19z 41 15 to to TO cord-323428-jd91k19z 41 16 express express VB cord-323428-jd91k19z 41 17 the the DT cord-323428-jd91k19z 41 18 S1 S1 NNP cord-323428-jd91k19z 41 19 domain domain NN cord-323428-jd91k19z 41 20 of of IN cord-323428-jd91k19z 41 21 the the DT cord-323428-jd91k19z 41 22 spike spike NN cord-323428-jd91k19z 41 23 protein protein NN cord-323428-jd91k19z 41 24 of of IN cord-323428-jd91k19z 41 25 MERS MERS NNP cord-323428-jd91k19z 41 26 - - HYPH cord-323428-jd91k19z 41 27 CoV CoV NNP cord-323428-jd91k19z 41 28 isolate isolate NN cord-323428-jd91k19z 42 1 Hu/ Hu/ NNP cord-323428-jd91k19z 42 2 Jordan-20140010168/2014 Jordan-20140010168/2014 NNPS cord-323428-jd91k19z 42 3 ( ( -LRB- cord-323428-jd91k19z 43 1 GenBank GenBank NNP cord-323428-jd91k19z 43 2 accession accession NN cord-323428-jd91k19z 43 3 number number NN cord-323428-jd91k19z 43 4 KT861627 kt861627 NN cord-323428-jd91k19z 43 5 ) ) -RRB- cord-323428-jd91k19z 44 1 under under IN cord-323428-jd91k19z 44 2 the the DT cord-323428-jd91k19z 44 3 control control NN cord-323428-jd91k19z 44 4 of of IN cord-323428-jd91k19z 44 5 the the DT cord-323428-jd91k19z 44 6 cytomegalovirus cytomegalovirus NNP cord-323428-jd91k19z 44 7 ( ( -LRB- cord-323428-jd91k19z 44 8 CMV CMV NNP cord-323428-jd91k19z 44 9 ) ) -RRB- cord-323428-jd91k19z 44 10 promoter promoter NN cord-323428-jd91k19z 44 11 . . . cord-323428-jd91k19z 45 1 This this DT cord-323428-jd91k19z 45 2 vaccine vaccine NN cord-323428-jd91k19z 45 3 was be VBD cord-323428-jd91k19z 45 4 constructed construct VBN cord-323428-jd91k19z 45 5 in in IN cord-323428-jd91k19z 45 6 the the DT cord-323428-jd91k19z 45 7 Laboratories Laboratories NNPS cord-323428-jd91k19z 45 8 of of IN cord-323428-jd91k19z 45 9 Vector Vector NNP cord-323428-jd91k19z 45 10 Biolabs Biolabs NNP cord-323428-jd91k19z 45 11 ( ( -LRB- cord-323428-jd91k19z 45 12 The the DT cord-323428-jd91k19z 45 13 Gene Gene NNP cord-323428-jd91k19z 45 14 Delivery Delivery NNP cord-323428-jd91k19z 45 15 Company Company NNP cord-323428-jd91k19z 45 16 , , , cord-323428-jd91k19z 45 17 PA PA NNP cord-323428-jd91k19z 45 18 , , , cord-323428-jd91k19z 45 19 USA USA NNP cord-323428-jd91k19z 45 20 ) ) -RRB- cord-323428-jd91k19z 45 21 [ [ -LRB- cord-323428-jd91k19z 45 22 28 28 CD cord-323428-jd91k19z 45 23 ] ] -RRB- cord-323428-jd91k19z 45 24 . . . cord-323428-jd91k19z 46 1 Six six CD cord-323428-jd91k19z 46 2 to to TO cord-323428-jd91k19z 46 3 eight eight CD cord-323428-jd91k19z 46 4 week week NN cord-323428-jd91k19z 46 5 - - HYPH cord-323428-jd91k19z 46 6 old old JJ cord-323428-jd91k19z 46 7 male male JJ cord-323428-jd91k19z 46 8 BALB BALB NNP cord-323428-jd91k19z 46 9 / / SYM cord-323428-jd91k19z 46 10 c c NN cord-323428-jd91k19z 46 11 mice mouse NNS cord-323428-jd91k19z 46 12 ( ( -LRB- cord-323428-jd91k19z 46 13 n=168 n=168 NNP cord-323428-jd91k19z 46 14 ) ) -RRB- cord-323428-jd91k19z 46 15 were be VBD cord-323428-jd91k19z 46 16 purchased purchase VBN cord-323428-jd91k19z 46 17 from from IN cord-323428-jd91k19z 46 18 Animal Animal NNP cord-323428-jd91k19z 46 19 House House NNP cord-323428-jd91k19z 46 20 at at IN cord-323428-jd91k19z 46 21 Jordan Jordan NNP cord-323428-jd91k19z 46 22 University University NNP cord-323428-jd91k19z 46 23 of of IN cord-323428-jd91k19z 46 24 Science Science NNP cord-323428-jd91k19z 46 25 and and CC cord-323428-jd91k19z 46 26 Technology Technology NNP cord-323428-jd91k19z 47 1 ( ( -LRB- cord-323428-jd91k19z 47 2 Irbid Irbid NNP cord-323428-jd91k19z 47 3 - - HYPH cord-323428-jd91k19z 47 4 Jordan Jordan NNP cord-323428-jd91k19z 47 5 ) ) -RRB- cord-323428-jd91k19z 47 6 and and CC cord-323428-jd91k19z 47 7 distributed distribute VBN cord-323428-jd91k19z 47 8 into into IN cord-323428-jd91k19z 47 9 three three CD cord-323428-jd91k19z 47 10 groups group NNS cord-323428-jd91k19z 47 11 in in IN cord-323428-jd91k19z 47 12 a a DT cord-323428-jd91k19z 47 13 replicate replicate NN cord-323428-jd91k19z 47 14 manner manner NN cord-323428-jd91k19z 47 15 ( ( -LRB- cord-323428-jd91k19z 47 16 56 56 CD cord-323428-jd91k19z 47 17 each each DT cord-323428-jd91k19z 47 18 ) ) -RRB- cord-323428-jd91k19z 47 19 . . . cord-323428-jd91k19z 48 1 Animals animal NNS cord-323428-jd91k19z 48 2 in in IN cord-323428-jd91k19z 48 3 the the DT cord-323428-jd91k19z 48 4 first first JJ cord-323428-jd91k19z 48 5 group group NN cord-323428-jd91k19z 48 6 were be VBD cord-323428-jd91k19z 48 7 immunized immunize VBN cord-323428-jd91k19z 48 8 with with IN cord-323428-jd91k19z 48 9 the the DT cord-323428-jd91k19z 48 10 Ad Ad NNP cord-323428-jd91k19z 48 11 - - HYPH cord-323428-jd91k19z 48 12 CMV CMV NNP cord-323428-jd91k19z 48 13 - - HYPH cord-323428-jd91k19z 48 14 MERS MERS NNP cord-323428-jd91k19z 48 15 - - HYPH cord-323428-jd91k19z 48 16 CoV CoV NNP cord-323428-jd91k19z 48 17 - - HYPH cord-323428-jd91k19z 48 18 Spike1 Spike1 NNP cord-323428-jd91k19z 48 19 " " '' cord-323428-jd91k19z 48 20 Ad ad NN cord-323428-jd91k19z 48 21 - - HYPH cord-323428-jd91k19z 48 22 MERS MERS NNP cord-323428-jd91k19z 48 23 - - HYPH cord-323428-jd91k19z 48 24 S1 S1 NNP cord-323428-jd91k19z 48 25 . . . cord-323428-jd91k19z 49 1 " " `` cord-323428-jd91k19z 49 2 Animals animal NNS cord-323428-jd91k19z 49 3 in in IN cord-323428-jd91k19z 49 4 the the DT cord-323428-jd91k19z 49 5 second second JJ cord-323428-jd91k19z 49 6 group group NN cord-323428-jd91k19z 49 7 received receive VBD cord-323428-jd91k19z 49 8 the the DT cord-323428-jd91k19z 49 9 vector vector NN cord-323428-jd91k19z 49 10 - - HYPH cord-323428-jd91k19z 49 11 only only RB cord-323428-jd91k19z 49 12 " " `` cord-323428-jd91k19z 49 13 Ad ad NN cord-323428-jd91k19z 49 14 - - HYPH cord-323428-jd91k19z 49 15 CMV CMV NNP cord-323428-jd91k19z 49 16 , , , cord-323428-jd91k19z 49 17 " " '' cord-323428-jd91k19z 49 18 and and CC cord-323428-jd91k19z 49 19 animals animal NNS cord-323428-jd91k19z 49 20 in in IN cord-323428-jd91k19z 49 21 the the DT cord-323428-jd91k19z 49 22 last last JJ cord-323428-jd91k19z 49 23 group group NN cord-323428-jd91k19z 49 24 served serve VBD cord-323428-jd91k19z 49 25 as as IN cord-323428-jd91k19z 49 26 a a DT cord-323428-jd91k19z 49 27 control control NN cord-323428-jd91k19z 49 28 and and CC cord-323428-jd91k19z 49 29 received receive VBD cord-323428-jd91k19z 49 30 1× 1× CD cord-323428-jd91k19z 49 31 phosphate phosphate NN cord-323428-jd91k19z 49 32 - - HYPH cord-323428-jd91k19z 49 33 buffered buffer VBN cord-323428-jd91k19z 49 34 saline saline NN cord-323428-jd91k19z 49 35 ( ( -LRB- cord-323428-jd91k19z 49 36 PBS PBS NNP cord-323428-jd91k19z 49 37 ) ) -RRB- cord-323428-jd91k19z 49 38 . . . cord-323428-jd91k19z 50 1 For for IN cord-323428-jd91k19z 50 2 the the DT cord-323428-jd91k19z 50 3 first first JJ cord-323428-jd91k19z 50 4 immunization immunization NN cord-323428-jd91k19z 50 5 , , , cord-323428-jd91k19z 50 6 the the DT cord-323428-jd91k19z 50 7 Ad ad NN cord-323428-jd91k19z 50 8 - - HYPH cord-323428-jd91k19z 50 9 MERS MERS NNP cord-323428-jd91k19z 50 10 - - HYPH cord-323428-jd91k19z 50 11 S1 S1 NNP cord-323428-jd91k19z 50 12 vaccine vaccine NN cord-323428-jd91k19z 50 13 was be VBD cord-323428-jd91k19z 50 14 injected inject VBN cord-323428-jd91k19z 50 15 intramuscularly intramuscularly RB cord-323428-jd91k19z 50 16 at at IN cord-323428-jd91k19z 50 17 a a DT cord-323428-jd91k19z 50 18 dose dose NN cord-323428-jd91k19z 50 19 of of IN cord-323428-jd91k19z 50 20 0.1 0.1 CD cord-323428-jd91k19z 50 21 ml ml XX cord-323428-jd91k19z 50 22 containing contain VBG cord-323428-jd91k19z 50 23 1×10 1×10 CD cord-323428-jd91k19z 50 24 7 7 CD cord-323428-jd91k19z 50 25 PFU/ pfu/ NN cord-323428-jd91k19z 50 26 dose dose NN cord-323428-jd91k19z 50 27 / / SYM cord-323428-jd91k19z 50 28 mouse mouse NN cord-323428-jd91k19z 50 29 . . . cord-323428-jd91k19z 51 1 The the DT cord-323428-jd91k19z 51 2 same same JJ cord-323428-jd91k19z 51 3 volume volume NN cord-323428-jd91k19z 51 4 of of IN cord-323428-jd91k19z 51 5 Ad ad NN cord-323428-jd91k19z 51 6 - - HYPH cord-323428-jd91k19z 51 7 CMV CMV NNP cord-323428-jd91k19z 51 8 or or CC cord-323428-jd91k19z 51 9 PBS PBS NNP cord-323428-jd91k19z 51 10 was be VBD cord-323428-jd91k19z 51 11 used use VBN cord-323428-jd91k19z 51 12 for for IN cord-323428-jd91k19z 51 13 the the DT cord-323428-jd91k19z 51 14 other other JJ cord-323428-jd91k19z 51 15 two two CD cord-323428-jd91k19z 51 16 groups group NNS cord-323428-jd91k19z 51 17 . . . cord-323428-jd91k19z 52 1 The the DT cord-323428-jd91k19z 52 2 booster booster NN cord-323428-jd91k19z 52 3 dose dose NN cord-323428-jd91k19z 52 4 was be VBD cord-323428-jd91k19z 52 5 carried carry VBN cord-323428-jd91k19z 52 6 out out RP cord-323428-jd91k19z 52 7 3 3 CD cord-323428-jd91k19z 52 8 weeks week NNS cord-323428-jd91k19z 52 9 later later RB cord-323428-jd91k19z 52 10 by by IN cord-323428-jd91k19z 52 11 intranasal intranasal NN cord-323428-jd91k19z 52 12 route route NN cord-323428-jd91k19z 52 13 at at IN cord-323428-jd91k19z 52 14 a a DT cord-323428-jd91k19z 52 15 dose dose NN cord-323428-jd91k19z 52 16 of of IN cord-323428-jd91k19z 52 17 0.1 0.1 CD cord-323428-jd91k19z 52 18 ml ml XX cord-323428-jd91k19z 52 19 containing contain VBG cord-323428-jd91k19z 52 20 1×10 1×10 CD cord-323428-jd91k19z 52 21 7 7 CD cord-323428-jd91k19z 52 22 PFU pfu NN cord-323428-jd91k19z 52 23 / / SYM cord-323428-jd91k19z 52 24 dose dose NN cord-323428-jd91k19z 52 25 / / SYM cord-323428-jd91k19z 52 26 mouse mouse NN cord-323428-jd91k19z 52 27 . . . cord-323428-jd91k19z 53 1 All all DT cord-323428-jd91k19z 53 2 experimental experimental JJ cord-323428-jd91k19z 53 3 procedures procedure NNS cord-323428-jd91k19z 53 4 used use VBN cord-323428-jd91k19z 53 5 in in IN cord-323428-jd91k19z 53 6 this this DT cord-323428-jd91k19z 53 7 study study NN cord-323428-jd91k19z 53 8 were be VBD cord-323428-jd91k19z 53 9 approved approve VBN cord-323428-jd91k19z 53 10 by by IN cord-323428-jd91k19z 53 11 the the DT cord-323428-jd91k19z 53 12 Animal Animal NNP cord-323428-jd91k19z 53 13 Care Care NNP cord-323428-jd91k19z 53 14 and and CC cord-323428-jd91k19z 53 15 Use Use NNP cord-323428-jd91k19z 53 16 Committee Committee NNP cord-323428-jd91k19z 53 17 at at IN cord-323428-jd91k19z 53 18 the the DT cord-323428-jd91k19z 53 19 Faculty Faculty NNP cord-323428-jd91k19z 53 20 of of IN cord-323428-jd91k19z 53 21 Veterinary Veterinary NNP cord-323428-jd91k19z 53 22 Medicine Medicine NNP cord-323428-jd91k19z 53 23 ( ( -LRB- cord-323428-jd91k19z 53 24 Irbid Irbid NNP cord-323428-jd91k19z 53 25 - - HYPH cord-323428-jd91k19z 53 26 Jordan Jordan NNP cord-323428-jd91k19z 53 27 ) ) -RRB- cord-323428-jd91k19z 53 28 . . . cord-323428-jd91k19z 54 1 Lung lung NN cord-323428-jd91k19z 54 2 , , , cord-323428-jd91k19z 54 3 kidney kidney NN cord-323428-jd91k19z 54 4 , , , cord-323428-jd91k19z 54 5 and and CC cord-323428-jd91k19z 54 6 spleen spleen NN cord-323428-jd91k19z 54 7 tissue tissue NN cord-323428-jd91k19z 54 8 samples sample NNS cord-323428-jd91k19z 54 9 were be VBD cord-323428-jd91k19z 54 10 harvested harvest VBN cord-323428-jd91k19z 54 11 from from IN cord-323428-jd91k19z 54 12 each each DT cord-323428-jd91k19z 54 13 mouse mouse NN cord-323428-jd91k19z 54 14 in in IN cord-323428-jd91k19z 54 15 all all DT cord-323428-jd91k19z 54 16 groups group NNS cord-323428-jd91k19z 54 17 after after IN cord-323428-jd91k19z 54 18 3 3 CD cord-323428-jd91k19z 54 19 , , , cord-323428-jd91k19z 54 20 4 4 CD cord-323428-jd91k19z 54 21 , , , cord-323428-jd91k19z 54 22 5 5 CD cord-323428-jd91k19z 54 23 , , , cord-323428-jd91k19z 54 24 and and CC cord-323428-jd91k19z 54 25 6 6 CD cord-323428-jd91k19z 54 26 weeks week NNS cord-323428-jd91k19z 54 27 from from IN cord-323428-jd91k19z 54 28 the the DT cord-323428-jd91k19z 54 29 first first JJ cord-323428-jd91k19z 54 30 immunization immunization NN cord-323428-jd91k19z 54 31 . . . cord-323428-jd91k19z 55 1 Parts part NNS cord-323428-jd91k19z 55 2 of of IN cord-323428-jd91k19z 55 3 lung lung NN cord-323428-jd91k19z 55 4 and and CC cord-323428-jd91k19z 55 5 kidneys kidney NNS cord-323428-jd91k19z 55 6 were be VBD cord-323428-jd91k19z 55 7 preserved preserve VBN cord-323428-jd91k19z 55 8 in in IN cord-323428-jd91k19z 55 9 10 10 CD cord-323428-jd91k19z 55 10 % % NN cord-323428-jd91k19z 55 11 formalin formalin NN cord-323428-jd91k19z 55 12 solution solution NN cord-323428-jd91k19z 55 13 for for IN cord-323428-jd91k19z 55 14 24-h 24-h JJ cord-323428-jd91k19z 55 15 , , , cord-323428-jd91k19z 55 16 then then RB cord-323428-jd91k19z 55 17 embedded embed VBN cord-323428-jd91k19z 55 18 in in IN cord-323428-jd91k19z 55 19 paraffin paraffin NN cord-323428-jd91k19z 55 20 blocks block NNS cord-323428-jd91k19z 55 21 for for IN cord-323428-jd91k19z 55 22 immunohistochemical immunohistochemical JJ cord-323428-jd91k19z 55 23 analysis analysis NN cord-323428-jd91k19z 55 24 , , , cord-323428-jd91k19z 55 25 while while IN cord-323428-jd91k19z 55 26 the the DT cord-323428-jd91k19z 55 27 other other JJ cord-323428-jd91k19z 55 28 parts part NNS cord-323428-jd91k19z 55 29 were be VBD cord-323428-jd91k19z 55 30 stored store VBN cord-323428-jd91k19z 55 31 at at IN cord-323428-jd91k19z 55 32 −80 −80 NNP cord-323428-jd91k19z 55 33 ° ° NN cord-323428-jd91k19z 55 34 C c NN cord-323428-jd91k19z 55 35 for for IN cord-323428-jd91k19z 55 36 polymerase polymerase NN cord-323428-jd91k19z 55 37 chain chain NN cord-323428-jd91k19z 55 38 reaction reaction NN cord-323428-jd91k19z 55 39 ( ( -LRB- cord-323428-jd91k19z 55 40 PCR PCR NNP cord-323428-jd91k19z 55 41 ) ) -RRB- cord-323428-jd91k19z 55 42 analysis analysis NN cord-323428-jd91k19z 55 43 . . . cord-323428-jd91k19z 56 1 Spleen spleen NN cord-323428-jd91k19z 56 2 tissues tissue NNS cord-323428-jd91k19z 56 3 were be VBD cord-323428-jd91k19z 56 4 harvested harvest VBN cord-323428-jd91k19z 56 5 and and CC cord-323428-jd91k19z 56 6 stored store VBN cord-323428-jd91k19z 56 7 at at IN cord-323428-jd91k19z 56 8 −80 −80 NNP cord-323428-jd91k19z 56 9 ° ° NN cord-323428-jd91k19z 56 10 C c NN cord-323428-jd91k19z 56 11 for for IN cord-323428-jd91k19z 56 12 cell cell NN cord-323428-jd91k19z 56 13 culture culture NN cord-323428-jd91k19z 56 14 analysis analysis NN cord-323428-jd91k19z 56 15 . . . cord-323428-jd91k19z 57 1 Blood blood NN cord-323428-jd91k19z 57 2 samples sample NNS cord-323428-jd91k19z 57 3 were be VBD cord-323428-jd91k19z 57 4 drawn draw VBN cord-323428-jd91k19z 57 5 through through IN cord-323428-jd91k19z 57 6 facial facial JJ cord-323428-jd91k19z 57 7 vein vein NN cord-323428-jd91k19z 57 8 route route NN cord-323428-jd91k19z 57 9 , , , cord-323428-jd91k19z 57 10 left leave VBD cord-323428-jd91k19z 57 11 30 30 CD cord-323428-jd91k19z 57 12 min min NN cord-323428-jd91k19z 57 13 at at IN cord-323428-jd91k19z 57 14 room room NN cord-323428-jd91k19z 57 15 temperature temperature NN cord-323428-jd91k19z 57 16 , , , cord-323428-jd91k19z 57 17 and and CC cord-323428-jd91k19z 57 18 centrifuged centrifuge VBN cord-323428-jd91k19z 57 19 at at IN cord-323428-jd91k19z 57 20 2000 2000 CD cord-323428-jd91k19z 57 21 rpm rpm NN cord-323428-jd91k19z 57 22 for for IN cord-323428-jd91k19z 57 23 20 20 CD cord-323428-jd91k19z 57 24 min min NN cord-323428-jd91k19z 57 25 . . . cord-323428-jd91k19z 58 1 Collected collect VBN cord-323428-jd91k19z 58 2 sera sera NNP cord-323428-jd91k19z 58 3 were be VBD cord-323428-jd91k19z 58 4 stored store VBN cord-323428-jd91k19z 58 5 at at IN cord-323428-jd91k19z 58 6 −20 −20 NNP cord-323428-jd91k19z 58 7 ° ° NN cord-323428-jd91k19z 58 8 C C NNP cord-323428-jd91k19z 58 9 for for IN cord-323428-jd91k19z 58 10 subsequent subsequent JJ cord-323428-jd91k19z 58 11 analysis analysis NN cord-323428-jd91k19z 58 12 . . . cord-323428-jd91k19z 59 1 To to TO cord-323428-jd91k19z 59 2 study study VB cord-323428-jd91k19z 59 3 the the DT cord-323428-jd91k19z 59 4 distribution distribution NN cord-323428-jd91k19z 59 5 of of IN cord-323428-jd91k19z 59 6 the the DT cord-323428-jd91k19z 59 7 recombinant recombinant JJ cord-323428-jd91k19z 59 8 vaccine vaccine NN cord-323428-jd91k19z 59 9 , , , cord-323428-jd91k19z 59 10 total total NN cord-323428-jd91k19z 59 11 of of IN cord-323428-jd91k19z 59 12 50 50 CD cord-323428-jd91k19z 59 13 - - SYM cord-323428-jd91k19z 59 14 100 100 CD cord-323428-jd91k19z 59 15 mg mg NNS cord-323428-jd91k19z 59 16 of of IN cord-323428-jd91k19z 59 17 lung lung NN cord-323428-jd91k19z 59 18 and and CC cord-323428-jd91k19z 59 19 kidney kidney NN cord-323428-jd91k19z 59 20 tissues tissue NNS cord-323428-jd91k19z 59 21 at at IN cord-323428-jd91k19z 59 22 weeks week NNS cord-323428-jd91k19z 59 23 3 3 CD cord-323428-jd91k19z 59 24 and and CC cord-323428-jd91k19z 59 25 5 5 CD cord-323428-jd91k19z 59 26 post post JJ cord-323428-jd91k19z 59 27 - - JJ cord-323428-jd91k19z 59 28 prime prime JJ cord-323428-jd91k19z 59 29 immunization immunization NN cord-323428-jd91k19z 59 30 was be VBD cord-323428-jd91k19z 59 31 cut cut VBN cord-323428-jd91k19z 59 32 and and CC cord-323428-jd91k19z 59 33 homogenized homogenize VBN cord-323428-jd91k19z 59 34 in in IN cord-323428-jd91k19z 59 35 1 1 CD cord-323428-jd91k19z 59 36 ml ml NNS cord-323428-jd91k19z 59 37 of of IN cord-323428-jd91k19z 59 38 diethyl diethyl NNP cord-323428-jd91k19z 59 39 pyrocarbonate pyrocarbonate NNP cord-323428-jd91k19z 59 40 - - HYPH cord-323428-jd91k19z 59 41 treated treat VBN cord-323428-jd91k19z 59 42 water water NN cord-323428-jd91k19z 59 43 . . . cord-323428-jd91k19z 60 1 Homogenized homogenized JJ cord-323428-jd91k19z 60 2 samples sample NNS cord-323428-jd91k19z 60 3 were be VBD cord-323428-jd91k19z 60 4 then then RB cord-323428-jd91k19z 60 5 centrifuged centrifuge VBN cord-323428-jd91k19z 60 6 at at IN cord-323428-jd91k19z 60 7 800 800 CD cord-323428-jd91k19z 60 8 rpm rpm NN cord-323428-jd91k19z 60 9 for for IN cord-323428-jd91k19z 60 10 5 5 CD cord-323428-jd91k19z 60 11 min min NN cord-323428-jd91k19z 60 12 , , , cord-323428-jd91k19z 60 13 and and CC cord-323428-jd91k19z 60 14 150 150 CD cord-323428-jd91k19z 60 15 µl µl IN cord-323428-jd91k19z 60 16 of of IN cord-323428-jd91k19z 60 17 the the DT cord-323428-jd91k19z 60 18 supernatant supernatant NN cord-323428-jd91k19z 60 19 was be VBD cord-323428-jd91k19z 60 20 used use VBN cord-323428-jd91k19z 60 21 for for IN cord-323428-jd91k19z 60 22 the the DT cord-323428-jd91k19z 60 23 extraction extraction NN cord-323428-jd91k19z 60 24 . . . cord-323428-jd91k19z 61 1 The the DT cord-323428-jd91k19z 61 2 extraction extraction NN cord-323428-jd91k19z 61 3 was be VBD cord-323428-jd91k19z 61 4 performed perform VBN cord-323428-jd91k19z 61 5 using use VBG cord-323428-jd91k19z 61 6 the the DT cord-323428-jd91k19z 61 7 Viral Viral NNP cord-323428-jd91k19z 61 8 Gene Gene NNP cord-323428-jd91k19z 61 9 - - HYPH cord-323428-jd91k19z 61 10 Spin Spin NNP cord-323428-jd91k19z 61 11 RNA RNA NNP cord-323428-jd91k19z 61 12 Extraction Extraction NNP cord-323428-jd91k19z 61 13 Kit kit NN cord-323428-jd91k19z 61 14 ( ( -LRB- cord-323428-jd91k19z 61 15 Intron Intron NNP cord-323428-jd91k19z 61 16 Biotechnology Biotechnology NNP cord-323428-jd91k19z 61 17 , , , cord-323428-jd91k19z 61 18 Korea Korea NNP cord-323428-jd91k19z 61 19 ) ) -RRB- cord-323428-jd91k19z 61 20 according accord VBG cord-323428-jd91k19z 61 21 to to IN cord-323428-jd91k19z 61 22 the the DT cord-323428-jd91k19z 61 23 manufacturer manufacturer NN cord-323428-jd91k19z 61 24 's 's POS cord-323428-jd91k19z 61 25 instructions instruction NNS cord-323428-jd91k19z 61 26 . . . cord-323428-jd91k19z 62 1 The the DT cord-323428-jd91k19z 62 2 extracted extract VBN cord-323428-jd91k19z 62 3 RNA RNA NNP cord-323428-jd91k19z 62 4 was be VBD cord-323428-jd91k19z 62 5 used use VBN cord-323428-jd91k19z 62 6 for for IN cord-323428-jd91k19z 62 7 cDNA cdna NN cord-323428-jd91k19z 62 8 synthesis synthesis NN cord-323428-jd91k19z 62 9 through through IN cord-323428-jd91k19z 62 10 a a DT cord-323428-jd91k19z 62 11 reverse reverse JJ cord-323428-jd91k19z 62 12 transcription transcription NN cord-323428-jd91k19z 62 13 system system NN cord-323428-jd91k19z 62 14 ( ( -LRB- cord-323428-jd91k19z 62 15 Promega Promega NNP cord-323428-jd91k19z 62 16 , , , cord-323428-jd91k19z 62 17 Madison Madison NNP cord-323428-jd91k19z 62 18 , , , cord-323428-jd91k19z 62 19 WI WI NNP cord-323428-jd91k19z 62 20 , , , cord-323428-jd91k19z 62 21 USA USA NNP cord-323428-jd91k19z 62 22 ) ) -RRB- cord-323428-jd91k19z 62 23 according accord VBG cord-323428-jd91k19z 62 24 to to IN cord-323428-jd91k19z 62 25 the the DT cord-323428-jd91k19z 62 26 manufacturer manufacturer NN cord-323428-jd91k19z 62 27 's 's POS cord-323428-jd91k19z 62 28 instructions instruction NNS cord-323428-jd91k19z 62 29 . . . cord-323428-jd91k19z 63 1 The the DT cord-323428-jd91k19z 63 2 cDNA cdna NN cord-323428-jd91k19z 63 3 was be VBD cord-323428-jd91k19z 63 4 kept keep VBN cord-323428-jd91k19z 63 5 at at IN cord-323428-jd91k19z 63 6 −20 −20 NNP cord-323428-jd91k19z 63 7 ° ° NN cord-323428-jd91k19z 63 8 C C NNP cord-323428-jd91k19z 63 9 until until IN cord-323428-jd91k19z 63 10 PCR PCR NNP cord-323428-jd91k19z 63 11 analysis analysis NN cord-323428-jd91k19z 63 12 . . . cord-323428-jd91k19z 64 1 The the DT cord-323428-jd91k19z 64 2 DNA dna NN cord-323428-jd91k19z 64 3 template template NN cord-323428-jd91k19z 64 4 of of IN cord-323428-jd91k19z 64 5 the the DT cord-323428-jd91k19z 64 6 constructed construct VBN cord-323428-jd91k19z 64 7 vaccine vaccine NN cord-323428-jd91k19z 64 8 was be VBD cord-323428-jd91k19z 64 9 also also RB cord-323428-jd91k19z 64 10 purified purify VBN cord-323428-jd91k19z 64 11 and and CC cord-323428-jd91k19z 64 12 used use VBN cord-323428-jd91k19z 64 13 as as IN cord-323428-jd91k19z 64 14 a a DT cord-323428-jd91k19z 64 15 positive positive JJ cord-323428-jd91k19z 64 16 control control NN cord-323428-jd91k19z 64 17 in in IN cord-323428-jd91k19z 64 18 this this DT cord-323428-jd91k19z 64 19 experiment experiment NN cord-323428-jd91k19z 64 20 . . . cord-323428-jd91k19z 65 1 Heminested Heminested NNP cord-323428-jd91k19z 65 2 PCR PCR NNP cord-323428-jd91k19z 65 3 was be VBD cord-323428-jd91k19z 65 4 carried carry VBN cord-323428-jd91k19z 65 5 out out RP cord-323428-jd91k19z 65 6 using use VBG cord-323428-jd91k19z 65 7 specific specific JJ cord-323428-jd91k19z 65 8 primers primer NNS cord-323428-jd91k19z 65 9 targeting target VBG cord-323428-jd91k19z 65 10 specific specific JJ cord-323428-jd91k19z 65 11 sequences sequence NNS cord-323428-jd91k19z 65 12 within within IN cord-323428-jd91k19z 65 13 the the DT cord-323428-jd91k19z 65 14 S1 S1 NNP cord-323428-jd91k19z 65 15 region region NN cord-323428-jd91k19z 65 16 , , , cord-323428-jd91k19z 65 17 as as IN cord-323428-jd91k19z 65 18 shown show VBN cord-323428-jd91k19z 65 19 in in IN cord-323428-jd91k19z 65 20 Table- Table- NNP cord-323428-jd91k19z 65 21 1 1 CD cord-323428-jd91k19z 65 22 [ [ -LRB- cord-323428-jd91k19z 65 23 29 29 CD cord-323428-jd91k19z 65 24 ] ] -RRB- cord-323428-jd91k19z 65 25 . . . cord-323428-jd91k19z 66 1 The the DT cord-323428-jd91k19z 66 2 first first JJ cord-323428-jd91k19z 66 3 PCR PCR NNP cord-323428-jd91k19z 66 4 targeted target VBD cord-323428-jd91k19z 66 5 a a DT cord-323428-jd91k19z 66 6 1172 1172 CD cord-323428-jd91k19z 66 7 bp bp NNP cord-323428-jd91k19z 66 8 sequence sequence NN cord-323428-jd91k19z 66 9 . . . cord-323428-jd91k19z 67 1 PCR PCR NNP cord-323428-jd91k19z 67 2 mixture mixture NN cord-323428-jd91k19z 67 3 contained contain VBD cord-323428-jd91k19z 67 4 0.4 0.4 CD cord-323428-jd91k19z 67 5 µM µm CD cord-323428-jd91k19z 67 6 of of IN cord-323428-jd91k19z 67 7 each each DT cord-323428-jd91k19z 67 8 primer primer NN cord-323428-jd91k19z 67 9 and and CC cord-323428-jd91k19z 67 10 using use VBG cord-323428-jd91k19z 67 11 3 3 CD cord-323428-jd91k19z 67 12 µl µl IN cord-323428-jd91k19z 67 13 of of IN cord-323428-jd91k19z 67 14 the the DT cord-323428-jd91k19z 67 15 cDNA cdna NN cord-323428-jd91k19z 67 16 template template NN cord-323428-jd91k19z 67 17 . . . cord-323428-jd91k19z 68 1 Touchdown Touchdown NNP cord-323428-jd91k19z 68 2 PCR PCR NNP cord-323428-jd91k19z 68 3 assay assay NN cord-323428-jd91k19z 68 4 was be VBD cord-323428-jd91k19z 68 5 programmed program VBN cord-323428-jd91k19z 68 6 on on IN cord-323428-jd91k19z 68 7 the the DT cord-323428-jd91k19z 68 8 MyCycler MyCycler NNP cord-323428-jd91k19z 68 9 thermal thermal JJ cord-323428-jd91k19z 68 10 cycler cycler NN cord-323428-jd91k19z 68 11 ( ( -LRB- cord-323428-jd91k19z 68 12 Bio Bio NNP cord-323428-jd91k19z 68 13 - - HYPH cord-323428-jd91k19z 68 14 Rad Rad NNP cord-323428-jd91k19z 68 15 Laboratories Laboratories NNP cord-323428-jd91k19z 68 16 , , , cord-323428-jd91k19z 68 17 Hercules Hercules NNP cord-323428-jd91k19z 68 18 , , , cord-323428-jd91k19z 68 19 CA CA NNP cord-323428-jd91k19z 68 20 , , , cord-323428-jd91k19z 68 21 USA USA NNP cord-323428-jd91k19z 68 22 ) ) -RRB- cord-323428-jd91k19z 68 23 as as IN cord-323428-jd91k19z 68 24 the the DT cord-323428-jd91k19z 68 25 following follow VBG cord-323428-jd91k19z 68 26 conditions condition NNS cord-323428-jd91k19z 68 27 : : : cord-323428-jd91k19z 68 28 95 95 CD cord-323428-jd91k19z 68 29 ° ° NN cord-323428-jd91k19z 68 30 C c NN cord-323428-jd91k19z 68 31 for for IN cord-323428-jd91k19z 68 32 10 10 CD cord-323428-jd91k19z 68 33 min min NN cord-323428-jd91k19z 68 34 , , , cord-323428-jd91k19z 68 35 followed follow VBN cord-323428-jd91k19z 68 36 by by IN cord-323428-jd91k19z 68 37 10 10 CD cord-323428-jd91k19z 68 38 cycles cycle NNS cord-323428-jd91k19z 68 39 of of IN cord-323428-jd91k19z 68 40 95 95 CD cord-323428-jd91k19z 68 41 ° ° NN cord-323428-jd91k19z 68 42 C c NN cord-323428-jd91k19z 68 43 for for IN cord-323428-jd91k19z 68 44 35 35 CD cord-323428-jd91k19z 68 45 s s NNS cord-323428-jd91k19z 68 46 , , , cord-323428-jd91k19z 68 47 63 63 CD cord-323428-jd91k19z 68 48 - - SYM cord-323428-jd91k19z 68 49 54 54 CD cord-323428-jd91k19z 68 50 ° ° NN cord-323428-jd91k19z 68 51 C c NN cord-323428-jd91k19z 68 52 for for IN cord-323428-jd91k19z 68 53 40 40 CD cord-323428-jd91k19z 68 54 s s NNS cord-323428-jd91k19z 68 55 , , , cord-323428-jd91k19z 68 56 and and CC cord-323428-jd91k19z 68 57 72 72 CD cord-323428-jd91k19z 68 58 ° ° NN cord-323428-jd91k19z 68 59 C c NN cord-323428-jd91k19z 68 60 for for IN cord-323428-jd91k19z 68 61 1 1 CD cord-323428-jd91k19z 68 62 min min NN cord-323428-jd91k19z 68 63 and and CC cord-323428-jd91k19z 68 64 15 15 CD cord-323428-jd91k19z 68 65 s s NNPS cord-323428-jd91k19z 68 66 , , , cord-323428-jd91k19z 68 67 then then RB cord-323428-jd91k19z 68 68 35 35 CD cord-323428-jd91k19z 68 69 cycles cycle NNS cord-323428-jd91k19z 68 70 of of IN cord-323428-jd91k19z 68 71 95 95 CD cord-323428-jd91k19z 68 72 ° ° NN cord-323428-jd91k19z 68 73 C c NN cord-323428-jd91k19z 68 74 for for IN cord-323428-jd91k19z 68 75 40 40 CD cord-323428-jd91k19z 68 76 s s NNS cord-323428-jd91k19z 68 77 , , , cord-323428-jd91k19z 68 78 56 56 CD cord-323428-jd91k19z 68 79 ° ° NN cord-323428-jd91k19z 68 80 C c NN cord-323428-jd91k19z 68 81 for for IN cord-323428-jd91k19z 68 82 40 40 CD cord-323428-jd91k19z 68 83 s s NNS cord-323428-jd91k19z 68 84 , , , cord-323428-jd91k19z 68 85 72 72 CD cord-323428-jd91k19z 68 86 ° ° NN cord-323428-jd91k19z 68 87 C c NN cord-323428-jd91k19z 68 88 for for IN cord-323428-jd91k19z 68 89 1 1 CD cord-323428-jd91k19z 68 90 min min NN cord-323428-jd91k19z 68 91 and and CC cord-323428-jd91k19z 68 92 15 15 CD cord-323428-jd91k19z 68 93 s s NNPS cord-323428-jd91k19z 68 94 , , , cord-323428-jd91k19z 68 95 and and CC cord-323428-jd91k19z 68 96 a a DT cord-323428-jd91k19z 68 97 final final JJ cord-323428-jd91k19z 68 98 extension extension NN cord-323428-jd91k19z 68 99 step step NN cord-323428-jd91k19z 68 100 at at IN cord-323428-jd91k19z 68 101 72 72 CD cord-323428-jd91k19z 68 102 ° ° NN cord-323428-jd91k19z 68 103 C c NN cord-323428-jd91k19z 68 104 for for IN cord-323428-jd91k19z 68 105 5 5 CD cord-323428-jd91k19z 68 106 min min NN cord-323428-jd91k19z 68 107 . . . cord-323428-jd91k19z 69 1 Two two CD cord-323428-jd91k19z 69 2 microliters microliter NNS cord-323428-jd91k19z 69 3 from from IN cord-323428-jd91k19z 69 4 the the DT cord-323428-jd91k19z 69 5 first first JJ cord-323428-jd91k19z 69 6 PCR PCR NNP cord-323428-jd91k19z 69 7 product product NN cord-323428-jd91k19z 69 8 were be VBD cord-323428-jd91k19z 69 9 used use VBN cord-323428-jd91k19z 69 10 as as IN cord-323428-jd91k19z 69 11 a a DT cord-323428-jd91k19z 69 12 template template NN cord-323428-jd91k19z 69 13 for for IN cord-323428-jd91k19z 69 14 the the DT cord-323428-jd91k19z 69 15 second second JJ cord-323428-jd91k19z 69 16 ( ( -LRB- cord-323428-jd91k19z 69 17 heminested heminested JJ cord-323428-jd91k19z 69 18 ) ) -RRB- cord-323428-jd91k19z 69 19 PCR PCR NNP cord-323428-jd91k19z 69 20 to to TO cord-323428-jd91k19z 69 21 amplify amplify VB cord-323428-jd91k19z 69 22 the the DT cord-323428-jd91k19z 69 23 1142 1142 CD cord-323428-jd91k19z 69 24 bp bp NNP cord-323428-jd91k19z 69 25 product product NN cord-323428-jd91k19z 69 26 following follow VBG cord-323428-jd91k19z 69 27 the the DT cord-323428-jd91k19z 69 28 first first JJ cord-323428-jd91k19z 69 29 PCR PCR NNP cord-323428-jd91k19z 69 30 conditions condition NNS cord-323428-jd91k19z 69 31 . . . cord-323428-jd91k19z 70 1 The the DT cord-323428-jd91k19z 70 2 bands band NNS cord-323428-jd91k19z 70 3 were be VBD cord-323428-jd91k19z 70 4 visualized visualize VBN cord-323428-jd91k19z 70 5 in in IN cord-323428-jd91k19z 70 6 1.5 1.5 CD cord-323428-jd91k19z 70 7 % % NN cord-323428-jd91k19z 70 8 agarose agarose JJ cord-323428-jd91k19z 70 9 gels gel NNS cord-323428-jd91k19z 70 10 using use VBG cord-323428-jd91k19z 70 11 an an DT cord-323428-jd91k19z 70 12 ultra ultra JJ cord-323428-jd91k19z 70 13 - - JJ cord-323428-jd91k19z 70 14 violet violet JJ cord-323428-jd91k19z 70 15 transilluminator transilluminator NN cord-323428-jd91k19z 70 16 . . . cord-323428-jd91k19z 71 1 Localization localization NN cord-323428-jd91k19z 71 2 of of IN cord-323428-jd91k19z 71 3 S1 S1 NNP cord-323428-jd91k19z 71 4 protein protein NN cord-323428-jd91k19z 71 5 expression expression NN cord-323428-jd91k19z 71 6 was be VBD cord-323428-jd91k19z 71 7 investigated investigate VBN cord-323428-jd91k19z 71 8 in in IN cord-323428-jd91k19z 71 9 both both DT cord-323428-jd91k19z 71 10 kidney kidney NN cord-323428-jd91k19z 71 11 and and CC cord-323428-jd91k19z 71 12 lung lung NN cord-323428-jd91k19z 71 13 tissues tissue NNS cord-323428-jd91k19z 71 14 from from IN cord-323428-jd91k19z 71 15 all all DT cord-323428-jd91k19z 71 16 groups group NNS cord-323428-jd91k19z 71 17 at at IN cord-323428-jd91k19z 71 18 week week NN cord-323428-jd91k19z 71 19 6 6 CD cord-323428-jd91k19z 71 20 after after IN cord-323428-jd91k19z 71 21 the the DT cord-323428-jd91k19z 71 22 first first JJ cord-323428-jd91k19z 71 23 vaccination vaccination NN cord-323428-jd91k19z 71 24 . . . cord-323428-jd91k19z 72 1 IHC IHC NNP cord-323428-jd91k19z 72 2 using use VBG cord-323428-jd91k19z 72 3 rabbit rabbit NN cord-323428-jd91k19z 72 4 - - HYPH cord-323428-jd91k19z 72 5 specific specific JJ cord-323428-jd91k19z 72 6 HRP HRP NNP cord-323428-jd91k19z 72 7 / / SYM cord-323428-jd91k19z 72 8 DAB DAB NNP cord-323428-jd91k19z 72 9 ( ( -LRB- cord-323428-jd91k19z 72 10 ABC ABC NNP cord-323428-jd91k19z 72 11 ) ) -RRB- cord-323428-jd91k19z 72 12 detection detection NN cord-323428-jd91k19z 72 13 IHC IHC NNP cord-323428-jd91k19z 72 14 kit kit NN cord-323428-jd91k19z 72 15 ( ( -LRB- cord-323428-jd91k19z 72 16 Abcam Abcam NNP cord-323428-jd91k19z 72 17 , , , cord-323428-jd91k19z 72 18 Cambridge Cambridge NNP cord-323428-jd91k19z 72 19 , , , cord-323428-jd91k19z 72 20 UK UK NNP cord-323428-jd91k19z 72 21 ) ) -RRB- cord-323428-jd91k19z 72 22 was be VBD cord-323428-jd91k19z 72 23 adapted adapt VBN cord-323428-jd91k19z 72 24 , , , cord-323428-jd91k19z 72 25 with with IN cord-323428-jd91k19z 72 26 using use VBG cord-323428-jd91k19z 72 27 antinovel antinovel NN cord-323428-jd91k19z 72 28 coronavirus coronavirus NN cord-323428-jd91k19z 72 29 spike spike NN cord-323428-jd91k19z 72 30 protein protein NN cord-323428-jd91k19z 72 31 S1 s1 NN cord-323428-jd91k19z 72 32 polyclonal polyclonal JJ cord-323428-jd91k19z 72 33 antibodies antibody NNS cord-323428-jd91k19z 72 34 ( ( -LRB- cord-323428-jd91k19z 72 35 1:1000 1:1000 NN cord-323428-jd91k19z 72 36 ; ; : cord-323428-jd91k19z 72 37 Sino sino JJ cord-323428-jd91k19z 72 38 - - HYPH cord-323428-jd91k19z 72 39 Biological Biological NNP cord-323428-jd91k19z 72 40 , , , cord-323428-jd91k19z 72 41 PA PA NNP cord-323428-jd91k19z 72 42 , , , cord-323428-jd91k19z 72 43 USA USA NNP cord-323428-jd91k19z 72 44 ) ) -RRB- cord-323428-jd91k19z 72 45 . . . cord-323428-jd91k19z 73 1 A a DT cord-323428-jd91k19z 73 2 5-µm 5-µm CD cord-323428-jd91k19z 73 3 thick thick JJ cord-323428-jd91k19z 73 4 section section NN cord-323428-jd91k19z 73 5 of of IN cord-323428-jd91k19z 73 6 formalin formalin NN cord-323428-jd91k19z 73 7 was be VBD cord-323428-jd91k19z 73 8 fixed fix VBN cord-323428-jd91k19z 73 9 , , , cord-323428-jd91k19z 73 10 and and CC cord-323428-jd91k19z 73 11 paraffin paraffin NN cord-323428-jd91k19z 73 12 - - HYPH cord-323428-jd91k19z 73 13 embedded embed VBN cord-323428-jd91k19z 73 14 tissues tissue NNS cord-323428-jd91k19z 73 15 were be VBD cord-323428-jd91k19z 73 16 cut cut VBN cord-323428-jd91k19z 73 17 and and CC cord-323428-jd91k19z 73 18 loaded load VBN cord-323428-jd91k19z 73 19 on on IN cord-323428-jd91k19z 73 20 specially specially RB cord-323428-jd91k19z 73 21 coated coat VBN cord-323428-jd91k19z 73 22 slides slide NNS cord-323428-jd91k19z 73 23 . . . cord-323428-jd91k19z 74 1 The the DT cord-323428-jd91k19z 74 2 prepared prepared JJ cord-323428-jd91k19z 74 3 slides slide NNS cord-323428-jd91k19z 74 4 were be VBD cord-323428-jd91k19z 74 5 put put VBN cord-323428-jd91k19z 74 6 in in IN cord-323428-jd91k19z 74 7 an an DT cord-323428-jd91k19z 74 8 oven oven NN cord-323428-jd91k19z 74 9 at at IN cord-323428-jd91k19z 74 10 70 70 CD cord-323428-jd91k19z 74 11 ° ° NN cord-323428-jd91k19z 74 12 C c NN cord-323428-jd91k19z 74 13 for for IN cord-323428-jd91k19z 74 14 30 30 CD cord-323428-jd91k19z 74 15 min min NN cord-323428-jd91k19z 74 16 followed follow VBN cord-323428-jd91k19z 74 17 by by IN cord-323428-jd91k19z 74 18 15 15 CD cord-323428-jd91k19z 74 19 min min NN cord-323428-jd91k19z 74 20 in in IN cord-323428-jd91k19z 74 21 a a DT cord-323428-jd91k19z 74 22 xylene xylene NN cord-323428-jd91k19z 74 23 solution solution NN cord-323428-jd91k19z 74 24 for for IN cord-323428-jd91k19z 74 25 deparaffinization deparaffinization NN cord-323428-jd91k19z 74 26 . . . cord-323428-jd91k19z 75 1 The the DT cord-323428-jd91k19z 75 2 sections section NNS cord-323428-jd91k19z 75 3 were be VBD cord-323428-jd91k19z 75 4 dried dry VBN cord-323428-jd91k19z 75 5 and and CC cord-323428-jd91k19z 75 6 surrounded surround VBN cord-323428-jd91k19z 75 7 by by IN cord-323428-jd91k19z 75 8 liquid liquid NN cord-323428-jd91k19z 75 9 blocker blocker NN cord-323428-jd91k19z 75 10 ( ( -LRB- cord-323428-jd91k19z 75 11 Dako Dako NNP cord-323428-jd91k19z 75 12 pen pen NN cord-323428-jd91k19z 75 13 ) ) -RRB- cord-323428-jd91k19z 75 14 . . . cord-323428-jd91k19z 76 1 The the DT cord-323428-jd91k19z 76 2 antigen antigen NN cord-323428-jd91k19z 76 3 retrieval retrieval NN cord-323428-jd91k19z 76 4 ( ( -LRB- cord-323428-jd91k19z 76 5 pretreatment pretreatment NN cord-323428-jd91k19z 76 6 ) ) -RRB- cord-323428-jd91k19z 76 7 step step NN cord-323428-jd91k19z 76 8 was be VBD cord-323428-jd91k19z 76 9 executed execute VBN cord-323428-jd91k19z 76 10 by by IN cord-323428-jd91k19z 76 11 immersing immerse VBG cord-323428-jd91k19z 76 12 the the DT cord-323428-jd91k19z 76 13 sections section NNS cord-323428-jd91k19z 76 14 in in IN cord-323428-jd91k19z 76 15 1× 1× CD cord-323428-jd91k19z 77 1 reveal reveal VB cord-323428-jd91k19z 77 2 Decloaker Decloaker NNP cord-323428-jd91k19z 77 3 reagent reagent NN cord-323428-jd91k19z 77 4 ( ( -LRB- cord-323428-jd91k19z 77 5 Biocare Biocare NNP cord-323428-jd91k19z 77 6 Medical Medical NNP cord-323428-jd91k19z 77 7 , , , cord-323428-jd91k19z 77 8 Pacheco Pacheco NNP cord-323428-jd91k19z 77 9 , , , cord-323428-jd91k19z 77 10 CA CA NNP cord-323428-jd91k19z 77 11 , , , cord-323428-jd91k19z 77 12 USA USA NNP cord-323428-jd91k19z 77 13 ) ) -RRB- cord-323428-jd91k19z 77 14 and and CC cord-323428-jd91k19z 77 15 heating heat VBG cord-323428-jd91k19z 77 16 in in IN cord-323428-jd91k19z 77 17 a a DT cord-323428-jd91k19z 77 18 microwave microwave NN cord-323428-jd91k19z 77 19 several several JJ cord-323428-jd91k19z 77 20 times time NNS cord-323428-jd91k19z 77 21 for for IN cord-323428-jd91k19z 77 22 30 30 CD cord-323428-jd91k19z 77 23 min min NN cord-323428-jd91k19z 78 1 then then RB cord-323428-jd91k19z 78 2 cool cool VB cord-323428-jd91k19z 78 3 at at IN cord-323428-jd91k19z 78 4 room room NN cord-323428-jd91k19z 78 5 temperature temperature NN cord-323428-jd91k19z 78 6 for for IN cord-323428-jd91k19z 78 7 15 15 CD cord-323428-jd91k19z 78 8 - - SYM cord-323428-jd91k19z 78 9 20 20 CD cord-323428-jd91k19z 78 10 min min NN cord-323428-jd91k19z 78 11 . . . cord-323428-jd91k19z 79 1 After after IN cord-323428-jd91k19z 79 2 that that DT cord-323428-jd91k19z 79 3 , , , cord-323428-jd91k19z 79 4 staining stain VBG cord-323428-jd91k19z 79 5 procedure procedure NN cord-323428-jd91k19z 79 6 was be VBD cord-323428-jd91k19z 79 7 carried carry VBN cord-323428-jd91k19z 79 8 out out RP cord-323428-jd91k19z 79 9 by by IN cord-323428-jd91k19z 79 10 following follow VBG cord-323428-jd91k19z 79 11 the the DT cord-323428-jd91k19z 79 12 manufacturer manufacturer NN cord-323428-jd91k19z 79 13 's 's POS cord-323428-jd91k19z 79 14 instructions instruction NNS cord-323428-jd91k19z 79 15 of of IN cord-323428-jd91k19z 79 16 the the DT cord-323428-jd91k19z 79 17 ABC ABC NNP cord-323428-jd91k19z 79 18 kit kit NN cord-323428-jd91k19z 79 19 . . . cord-323428-jd91k19z 80 1 To to TO cord-323428-jd91k19z 80 2 eliminate eliminate VB cord-323428-jd91k19z 80 3 the the DT cord-323428-jd91k19z 80 4 effect effect NN cord-323428-jd91k19z 80 5 of of IN cord-323428-jd91k19z 80 6 internal internal JJ cord-323428-jd91k19z 80 7 renal renal JJ cord-323428-jd91k19z 80 8 biotin biotin NN cord-323428-jd91k19z 80 9 , , , cord-323428-jd91k19z 80 10 0.05 0.05 CD cord-323428-jd91k19z 80 11 % % NN cord-323428-jd91k19z 80 12 avidin avidin NN cord-323428-jd91k19z 80 13 was be VBD cord-323428-jd91k19z 80 14 applied apply VBN cord-323428-jd91k19z 80 15 ( ( -LRB- cord-323428-jd91k19z 80 16 Sigma Sigma NNP cord-323428-jd91k19z 80 17 - - HYPH cord-323428-jd91k19z 80 18 Aldrich Aldrich NNP cord-323428-jd91k19z 80 19 , , , cord-323428-jd91k19z 80 20 St. St. NNP cord-323428-jd91k19z 80 21 Louis Louis NNP cord-323428-jd91k19z 80 22 , , , cord-323428-jd91k19z 80 23 MO MO NNP cord-323428-jd91k19z 80 24 , , , cord-323428-jd91k19z 80 25 USA USA NNP cord-323428-jd91k19z 80 26 ) ) -RRB- cord-323428-jd91k19z 80 27 only only RB cord-323428-jd91k19z 80 28 on on IN cord-323428-jd91k19z 80 29 kidney kidney NN cord-323428-jd91k19z 80 30 sections section NNS cord-323428-jd91k19z 80 31 for for IN cord-323428-jd91k19z 80 32 15 15 CD cord-323428-jd91k19z 80 33 min min NN cord-323428-jd91k19z 80 34 at at IN cord-323428-jd91k19z 80 35 room room NN cord-323428-jd91k19z 80 36 temperature temperature NN cord-323428-jd91k19z 80 37 [ [ -LRB- cord-323428-jd91k19z 80 38 30 30 CD cord-323428-jd91k19z 80 39 , , , cord-323428-jd91k19z 80 40 31 31 CD cord-323428-jd91k19z 80 41 ] ] -RRB- cord-323428-jd91k19z 80 42 . . . cord-323428-jd91k19z 81 1 The the DT cord-323428-jd91k19z 81 2 immunoreactivity immunoreactivity NN cord-323428-jd91k19z 81 3 of of IN cord-323428-jd91k19z 81 4 the the DT cord-323428-jd91k19z 81 5 expressed express VBN cord-323428-jd91k19z 81 6 S1 S1 NNP cord-323428-jd91k19z 81 7 protein protein NN cord-323428-jd91k19z 81 8 was be VBD cord-323428-jd91k19z 81 9 visualized visualize VBN cord-323428-jd91k19z 81 10 using use VBG cord-323428-jd91k19z 81 11 a a DT cord-323428-jd91k19z 81 12 light light JJ cord-323428-jd91k19z 81 13 microscope microscope NN cord-323428-jd91k19z 81 14 at at IN cord-323428-jd91k19z 81 15 different different JJ cord-323428-jd91k19z 81 16 magnifications magnification NNS cord-323428-jd91k19z 81 17 . . . cord-323428-jd91k19z 82 1 Spleen spleen NN cord-323428-jd91k19z 82 2 tissues tissue NNS cord-323428-jd91k19z 82 3 from from IN cord-323428-jd91k19z 82 4 each each DT cord-323428-jd91k19z 82 5 group group NN cord-323428-jd91k19z 82 6 were be VBD cord-323428-jd91k19z 82 7 collected collect VBN cord-323428-jd91k19z 82 8 , , , cord-323428-jd91k19z 82 9 pooled pool VBN cord-323428-jd91k19z 82 10 , , , cord-323428-jd91k19z 82 11 and and CC cord-323428-jd91k19z 82 12 homogenized homogenize VBN cord-323428-jd91k19z 82 13 to to TO cord-323428-jd91k19z 82 14 obtain obtain VB cord-323428-jd91k19z 82 15 single single JJ cord-323428-jd91k19z 82 16 - - HYPH cord-323428-jd91k19z 82 17 cell cell NN cord-323428-jd91k19z 82 18 suspensions suspension NNS cord-323428-jd91k19z 82 19 . . . cord-323428-jd91k19z 83 1 The the DT cord-323428-jd91k19z 83 2 cells cell NNS cord-323428-jd91k19z 83 3 were be VBD cord-323428-jd91k19z 83 4 centrifuged centrifuge VBN cord-323428-jd91k19z 83 5 for for IN cord-323428-jd91k19z 83 6 10 10 CD cord-323428-jd91k19z 83 7 min min NN cord-323428-jd91k19z 83 8 at at IN cord-323428-jd91k19z 83 9 800 800 CD cord-323428-jd91k19z 83 10 rpm rpm NN cord-323428-jd91k19z 83 11 , , , cord-323428-jd91k19z 83 12 and and CC cord-323428-jd91k19z 83 13 the the DT cord-323428-jd91k19z 83 14 pellet pellet NN cord-323428-jd91k19z 83 15 was be VBD cord-323428-jd91k19z 83 16 re re VBN cord-323428-jd91k19z 83 17 - - VBN cord-323428-jd91k19z 83 18 suspended suspend VBN cord-323428-jd91k19z 83 19 in in IN cord-323428-jd91k19z 83 20 Roswell Roswell NNP cord-323428-jd91k19z 83 21 Park Park NNP cord-323428-jd91k19z 83 22 Memorial Memorial NNP cord-323428-jd91k19z 83 23 Institute Institute NNP cord-323428-jd91k19z 83 24 Medium Medium NNP cord-323428-jd91k19z 83 25 ( ( -LRB- cord-323428-jd91k19z 83 26 RPMI RPMI NNP cord-323428-jd91k19z 83 27 ) ) -RRB- cord-323428-jd91k19z 84 1 ( ( -LRB- cord-323428-jd91k19z 84 2 Gibco Gibco NNP cord-323428-jd91k19z 84 3 , , , cord-323428-jd91k19z 84 4 Grand Grand NNP cord-323428-jd91k19z 84 5 Island Island NNP cord-323428-jd91k19z 84 6 , , , cord-323428-jd91k19z 84 7 NY NY NNP cord-323428-jd91k19z 84 8 , , , cord-323428-jd91k19z 84 9 USA USA NNP cord-323428-jd91k19z 84 10 ) ) -RRB- cord-323428-jd91k19z 84 11 , , , cord-323428-jd91k19z 84 12 supplemented supplement VBN cord-323428-jd91k19z 84 13 with with IN cord-323428-jd91k19z 84 14 10 10 CD cord-323428-jd91k19z 84 15 % % NN cord-323428-jd91k19z 84 16 fetal fetal JJ cord-323428-jd91k19z 84 17 calf calf NN cord-323428-jd91k19z 84 18 serum serum NN cord-323428-jd91k19z 84 19 , , , cord-323428-jd91k19z 84 20 20 20 CD cord-323428-jd91k19z 84 21 mM mM NNP cord-323428-jd91k19z 84 22 HEPES HEPES NNP cord-323428-jd91k19z 84 23 , , , cord-323428-jd91k19z 84 24 10 10 CD cord-323428-jd91k19z 84 25 µg µg NN cord-323428-jd91k19z 84 26 / / SYM cord-323428-jd91k19z 84 27 ml ml IN cord-323428-jd91k19z 84 28 penicillin penicillin NN cord-323428-jd91k19z 84 29 / / SYM cord-323428-jd91k19z 84 30 streptomycin streptomycin NNS cord-323428-jd91k19z 84 31 , , , cord-323428-jd91k19z 84 32 2 2 CD cord-323428-jd91k19z 84 33 mM mm CD cord-323428-jd91k19z 84 34 L l NN cord-323428-jd91k19z 84 35 - - HYPH cord-323428-jd91k19z 84 36 glutamine glutamine NN cord-323428-jd91k19z 84 37 , , , cord-323428-jd91k19z 84 38 50 50 CD cord-323428-jd91k19z 84 39 µM µm CD cord-323428-jd91k19z 84 40 2-mercaptoethanol 2-mercaptoethanol CD cord-323428-jd91k19z 84 41 , , , cord-323428-jd91k19z 84 42 and and CC cord-323428-jd91k19z 84 43 500 500 CD cord-323428-jd91k19z 84 44 ng ng NN cord-323428-jd91k19z 84 45 / / SYM cord-323428-jd91k19z 84 46 ml ml NN cord-323428-jd91k19z 85 1 Amphiostat Amphiostat NNP cord-323428-jd91k19z 85 2 B B NNP cord-323428-jd91k19z 85 3 ( ( -LRB- cord-323428-jd91k19z 85 4 complete complete JJ cord-323428-jd91k19z 85 5 RPMI RPMI NNP cord-323428-jd91k19z 85 6 ) ) -RRB- cord-323428-jd91k19z 86 1 [ [ -LRB- cord-323428-jd91k19z 86 2 32 32 CD cord-323428-jd91k19z 86 3 ] ] -RRB- cord-323428-jd91k19z 86 4 . . . cord-323428-jd91k19z 87 1 The the DT cord-323428-jd91k19z 87 2 cell cell NN cord-323428-jd91k19z 87 3 suspension suspension NN cord-323428-jd91k19z 87 4 was be VBD cord-323428-jd91k19z 87 5 washed wash VBN cord-323428-jd91k19z 87 6 again again RB cord-323428-jd91k19z 87 7 by by IN cord-323428-jd91k19z 87 8 centrifugation centrifugation NN cord-323428-jd91k19z 87 9 as as IN cord-323428-jd91k19z 87 10 described describe VBN cord-323428-jd91k19z 87 11 earlier early RBR cord-323428-jd91k19z 87 12 . . . cord-323428-jd91k19z 88 1 Spleen spleen NN cord-323428-jd91k19z 88 2 cells cell NNS cord-323428-jd91k19z 88 3 were be VBD cord-323428-jd91k19z 88 4 re re VBN cord-323428-jd91k19z 88 5 - - VBN cord-323428-jd91k19z 88 6 suspended suspend VBN cord-323428-jd91k19z 88 7 in in IN cord-323428-jd91k19z 88 8 red red JJ cord-323428-jd91k19z 88 9 blood blood NN cord-323428-jd91k19z 88 10 cell cell NN cord-323428-jd91k19z 88 11 lysis lysis NN cord-323428-jd91k19z 88 12 buffer buffer NN cord-323428-jd91k19z 88 13 ( ( -LRB- cord-323428-jd91k19z 88 14 Trisbuffered Trisbuffered NNP cord-323428-jd91k19z 88 15 NH NH NNP cord-323428-jd91k19z 88 16 4 4 CD cord-323428-jd91k19z 88 17 CL CL NNP cord-323428-jd91k19z 88 18 ) ) -RRB- cord-323428-jd91k19z 88 19 to to TO cord-323428-jd91k19z 88 20 remove remove VB cord-323428-jd91k19z 88 21 erythrocytes erythrocyte NNS cord-323428-jd91k19z 88 22 . . . cord-323428-jd91k19z 89 1 Cells cell NNS cord-323428-jd91k19z 89 2 from from IN cord-323428-jd91k19z 89 3 each each DT cord-323428-jd91k19z 89 4 group group NN cord-323428-jd91k19z 89 5 ( ( -LRB- cord-323428-jd91k19z 89 6 1×10 1×10 CD cord-323428-jd91k19z 89 7 5 5 CD cord-323428-jd91k19z 89 8 ) ) -RRB- cord-323428-jd91k19z 89 9 were be VBD cord-323428-jd91k19z 89 10 re re VBN cord-323428-jd91k19z 89 11 - - VBN cord-323428-jd91k19z 89 12 suspended suspend VBN cord-323428-jd91k19z 89 13 in in IN cord-323428-jd91k19z 89 14 1 1 CD cord-323428-jd91k19z 89 15 ml ml NNS cord-323428-jd91k19z 89 16 of of IN cord-323428-jd91k19z 89 17 complete complete JJ cord-323428-jd91k19z 89 18 RPMI RPMI NNP cord-323428-jd91k19z 89 19 and and CC cord-323428-jd91k19z 89 20 plated plate VBN cord-323428-jd91k19z 89 21 in in IN cord-323428-jd91k19z 89 22 24-well 24-well CD cord-323428-jd91k19z 89 23 plates plate NNS cord-323428-jd91k19z 89 24 in in IN cord-323428-jd91k19z 89 25 triplicate triplicate NN cord-323428-jd91k19z 89 26 in in IN cord-323428-jd91k19z 89 27 parallel parallel JJ cord-323428-jd91k19z 89 28 , , , cord-323428-jd91k19z 89 29 adjacent adjacent JJ cord-323428-jd91k19z 89 30 wells well NNS cord-323428-jd91k19z 89 31 . . . cord-323428-jd91k19z 90 1 The the DT cord-323428-jd91k19z 90 2 first first JJ cord-323428-jd91k19z 90 3 set set NN cord-323428-jd91k19z 90 4 of of IN cord-323428-jd91k19z 90 5 samples sample NNS cord-323428-jd91k19z 90 6 was be VBD cord-323428-jd91k19z 90 7 stimulated stimulate VBN cord-323428-jd91k19z 90 8 with with IN cord-323428-jd91k19z 90 9 10 10 CD cord-323428-jd91k19z 90 10 µg µg NN cord-323428-jd91k19z 90 11 of of IN cord-323428-jd91k19z 90 12 specific specific JJ cord-323428-jd91k19z 90 13 peptide peptide NN cord-323428-jd91k19z 90 14 " " `` cord-323428-jd91k19z 90 15 epitope epitope NN cord-323428-jd91k19z 90 16 " " `` cord-323428-jd91k19z 90 17 CYSSLILDY cysslildy NN cord-323428-jd91k19z 90 18 per per IN cord-323428-jd91k19z 90 19 well well RB cord-323428-jd91k19z 90 20 . . . cord-323428-jd91k19z 91 1 This this DT cord-323428-jd91k19z 91 2 antigen antigen NN cord-323428-jd91k19z 91 3 was be VBD cord-323428-jd91k19z 91 4 defined define VBN cord-323428-jd91k19z 91 5 as as IN cord-323428-jd91k19z 91 6 the the DT cord-323428-jd91k19z 91 7 highest high JJS cord-323428-jd91k19z 91 8 potential potential JJ cord-323428-jd91k19z 91 9 T t NN cord-323428-jd91k19z 91 10 - - HYPH cord-323428-jd91k19z 91 11 cell cell NN cord-323428-jd91k19z 91 12 epitope epitope NN cord-323428-jd91k19z 91 13 from from IN cord-323428-jd91k19z 91 14 a a DT cord-323428-jd91k19z 91 15 total total NN cord-323428-jd91k19z 91 16 of of IN cord-323428-jd91k19z 91 17 eight eight CD cord-323428-jd91k19z 91 18 potential potential JJ cord-323428-jd91k19z 91 19 epitopes epitope NNS cord-323428-jd91k19z 91 20 within within IN cord-323428-jd91k19z 91 21 the the DT cord-323428-jd91k19z 91 22 RBD RBD NNP cord-323428-jd91k19z 91 23 region region NN cord-323428-jd91k19z 91 24 of of IN cord-323428-jd91k19z 91 25 the the DT cord-323428-jd91k19z 91 26 S1 S1 NNP cord-323428-jd91k19z 91 27 subunit subunit NN cord-323428-jd91k19z 91 28 of of IN cord-323428-jd91k19z 91 29 the the DT cord-323428-jd91k19z 91 30 spike spike NN cord-323428-jd91k19z 91 31 protein protein NN cord-323428-jd91k19z 91 32 of of IN cord-323428-jd91k19z 91 33 MERS MERS NNP cord-323428-jd91k19z 91 34 - - HYPH cord-323428-jd91k19z 91 35 CoV CoV NNP cord-323428-jd91k19z 91 36 genome genome NN cord-323428-jd91k19z 91 37 and and CC cord-323428-jd91k19z 91 38 was be VBD cord-323428-jd91k19z 91 39 expected expect VBN cord-323428-jd91k19z 91 40 to to TO cord-323428-jd91k19z 91 41 display display VB cord-323428-jd91k19z 91 42 interactions interaction NNS cord-323428-jd91k19z 91 43 with with IN cord-323428-jd91k19z 91 44 the the DT cord-323428-jd91k19z 91 45 maximum maximum JJ cord-323428-jd91k19z 91 46 number number NN cord-323428-jd91k19z 91 47 of of IN cord-323428-jd91k19z 91 48 major major JJ cord-323428-jd91k19z 91 49 histocompatibility histocompatibility NN cord-323428-jd91k19z 91 50 complex complex NN cord-323428-jd91k19z 91 51 ( ( -LRB- cord-323428-jd91k19z 91 52 MHC MHC NNP cord-323428-jd91k19z 91 53 ) ) -RRB- cord-323428-jd91k19z 91 54 Class class NN cord-323428-jd91k19z 91 55 I -PRON- PRP cord-323428-jd91k19z 91 56 molecules molecule NNS cord-323428-jd91k19z 91 57 , , , cord-323428-jd91k19z 91 58 and and CC cord-323428-jd91k19z 91 59 to to TO cord-323428-jd91k19z 91 60 show show VB cord-323428-jd91k19z 91 61 the the DT cord-323428-jd91k19z 91 62 highest high JJS cord-323428-jd91k19z 91 63 peak peak NN cord-323428-jd91k19z 91 64 in in IN cord-323428-jd91k19z 91 65 the the DT cord-323428-jd91k19z 91 66 B b NN cord-323428-jd91k19z 91 67 - - HYPH cord-323428-jd91k19z 91 68 cell cell NN cord-323428-jd91k19z 91 69 antigenicity antigenicity NN cord-323428-jd91k19z 91 70 plot plot NN cord-323428-jd91k19z 91 71 [ [ -LRB- cord-323428-jd91k19z 91 72 33 33 CD cord-323428-jd91k19z 91 73 ] ] -RRB- cord-323428-jd91k19z 91 74 . . . cord-323428-jd91k19z 92 1 The the DT cord-323428-jd91k19z 92 2 second second JJ cord-323428-jd91k19z 92 3 set set NN cord-323428-jd91k19z 92 4 of of IN cord-323428-jd91k19z 92 5 samples sample NNS cord-323428-jd91k19z 92 6 was be VBD cord-323428-jd91k19z 92 7 stimulated stimulate VBN cord-323428-jd91k19z 92 8 with with IN cord-323428-jd91k19z 92 9 50 50 CD cord-323428-jd91k19z 92 10 µg µg NNS cord-323428-jd91k19z 92 11 of of IN cord-323428-jd91k19z 92 12 non non JJ cord-323428-jd91k19z 92 13 - - JJ cord-323428-jd91k19z 92 14 specific specific JJ cord-323428-jd91k19z 92 15 stimulant stimulant NN cord-323428-jd91k19z 92 16 phytohemagglutinin phytohemagglutinin NN cord-323428-jd91k19z 92 17 to to TO cord-323428-jd91k19z 92 18 confirm confirm VB cord-323428-jd91k19z 92 19 the the DT cord-323428-jd91k19z 92 20 viability viability NN cord-323428-jd91k19z 92 21 and and CC cord-323428-jd91k19z 92 22 productivity productivity NN cord-323428-jd91k19z 92 23 of of IN cord-323428-jd91k19z 92 24 the the DT cord-323428-jd91k19z 92 25 cultured cultured JJ cord-323428-jd91k19z 92 26 cells cell NNS cord-323428-jd91k19z 92 27 . . . cord-323428-jd91k19z 93 1 The the DT cord-323428-jd91k19z 93 2 third third JJ cord-323428-jd91k19z 93 3 set set NN cord-323428-jd91k19z 93 4 of of IN cord-323428-jd91k19z 93 5 samples sample NNS cord-323428-jd91k19z 93 6 was be VBD cord-323428-jd91k19z 93 7 not not RB cord-323428-jd91k19z 93 8 stimulated stimulate VBN cord-323428-jd91k19z 93 9 as as IN cord-323428-jd91k19z 93 10 a a DT cord-323428-jd91k19z 93 11 control control NN cord-323428-jd91k19z 93 12 set set NN cord-323428-jd91k19z 93 13 . . . cord-323428-jd91k19z 94 1 Cells cell NNS cord-323428-jd91k19z 94 2 were be VBD cord-323428-jd91k19z 94 3 incubated incubate VBN cord-323428-jd91k19z 94 4 in in IN cord-323428-jd91k19z 94 5 round round JJ cord-323428-jd91k19z 94 6 - - HYPH cord-323428-jd91k19z 94 7 bottom bottom NN cord-323428-jd91k19z 94 8 24-well 24-well CD cord-323428-jd91k19z 94 9 microtiter microtiter NN cord-323428-jd91k19z 94 10 plates plate NNS cord-323428-jd91k19z 94 11 at at IN cord-323428-jd91k19z 94 12 37 37 CD cord-323428-jd91k19z 94 13 ° ° NN cord-323428-jd91k19z 94 14 C c NN cord-323428-jd91k19z 94 15 in in IN cord-323428-jd91k19z 94 16 5 5 CD cord-323428-jd91k19z 94 17 % % NN cord-323428-jd91k19z 94 18 CO co NN cord-323428-jd91k19z 94 19 2 2 CD cord-323428-jd91k19z 94 20 . . . cord-323428-jd91k19z 95 1 The the DT cord-323428-jd91k19z 95 2 cultured cultured JJ cord-323428-jd91k19z 95 3 media medium NNS cord-323428-jd91k19z 95 4 were be VBD cord-323428-jd91k19z 95 5 collected collect VBN cord-323428-jd91k19z 95 6 after after IN cord-323428-jd91k19z 95 7 96 96 CD cord-323428-jd91k19z 95 8 h h NN cord-323428-jd91k19z 95 9 , , , cord-323428-jd91k19z 95 10 and and CC cord-323428-jd91k19z 95 11 the the DT cord-323428-jd91k19z 95 12 samples sample NNS cord-323428-jd91k19z 95 13 were be VBD cord-323428-jd91k19z 95 14 centrifuged centrifuge VBN cord-323428-jd91k19z 95 15 for for IN cord-323428-jd91k19z 95 16 20 20 CD cord-323428-jd91k19z 95 17 min min NN cord-323428-jd91k19z 95 18 at at IN cord-323428-jd91k19z 95 19 2000 2000 CD cord-323428-jd91k19z 95 20 rpm rpm NN cord-323428-jd91k19z 95 21 . . . cord-323428-jd91k19z 96 1 The the DT cord-323428-jd91k19z 96 2 supernatants supernatant NNS cord-323428-jd91k19z 96 3 were be VBD cord-323428-jd91k19z 96 4 stored store VBN cord-323428-jd91k19z 96 5 at at IN cord-323428-jd91k19z 96 6 −20 −20 NNP cord-323428-jd91k19z 96 7 ° ° NN cord-323428-jd91k19z 96 8 C C NNP cord-323428-jd91k19z 96 9 before before IN cord-323428-jd91k19z 96 10 cytokine cytokine NN cord-323428-jd91k19z 96 11 analysis analysis NN cord-323428-jd91k19z 96 12 , , , cord-323428-jd91k19z 96 13 while while IN cord-323428-jd91k19z 96 14 the the DT cord-323428-jd91k19z 96 15 pellets pellet NNS cord-323428-jd91k19z 96 16 were be VBD cord-323428-jd91k19z 96 17 stored store VBN cord-323428-jd91k19z 96 18 at at IN cord-323428-jd91k19z 96 19 −80 −80 NNP cord-323428-jd91k19z 96 20 ° ° NN cord-323428-jd91k19z 96 21 C c NN cord-323428-jd91k19z 96 22 until until IN cord-323428-jd91k19z 96 23 real real JJ cord-323428-jd91k19z 96 24 - - HYPH cord-323428-jd91k19z 96 25 time time NN cord-323428-jd91k19z 96 26 ( ( -LRB- cord-323428-jd91k19z 96 27 RT)-PCR rt)-pcr NN cord-323428-jd91k19z 96 28 analysis analysis NN cord-323428-jd91k19z 96 29 . . . cord-323428-jd91k19z 97 1 Detection detection NN cord-323428-jd91k19z 97 2 and and CC cord-323428-jd91k19z 97 3 measurement measurement NN cord-323428-jd91k19z 97 4 of of IN cord-323428-jd91k19z 97 5 specific specific JJ cord-323428-jd91k19z 97 6 MERS MERS NNP cord-323428-jd91k19z 97 7 - - HYPH cord-323428-jd91k19z 97 8 S1 S1 NNP cord-323428-jd91k19z 97 9 IgG igg NN cord-323428-jd91k19z 97 10 were be VBD cord-323428-jd91k19z 97 11 achieved achieve VBN cord-323428-jd91k19z 97 12 using use VBG cord-323428-jd91k19z 97 13 a a DT cord-323428-jd91k19z 97 14 semi semi JJ cord-323428-jd91k19z 97 15 - - JJ cord-323428-jd91k19z 97 16 quantitative quantitative JJ cord-323428-jd91k19z 97 17 anti anti JJ cord-323428-jd91k19z 97 18 - - JJ cord-323428-jd91k19z 97 19 MERS mers JJ cord-323428-jd91k19z 97 20 - - HYPH cord-323428-jd91k19z 97 21 CoV CoV NNP cord-323428-jd91k19z 97 22 ELISA ELISA NNP cord-323428-jd91k19z 97 23 ( ( -LRB- cord-323428-jd91k19z 97 24 IgG IgG NNP cord-323428-jd91k19z 97 25 ) ) -RRB- cord-323428-jd91k19z 97 26 ( ( -LRB- cord-323428-jd91k19z 97 27 EUROIMMUN EUROIMMUN NNP cord-323428-jd91k19z 97 28 AG AG NNP cord-323428-jd91k19z 97 29 , , , cord-323428-jd91k19z 97 30 Luebeck Luebeck NNP cord-323428-jd91k19z 97 31 , , , cord-323428-jd91k19z 97 32 Germany Germany NNP cord-323428-jd91k19z 97 33 ) ) -RRB- cord-323428-jd91k19z 97 34 with with IN cord-323428-jd91k19z 97 35 a a DT cord-323428-jd91k19z 97 36 slight slight JJ cord-323428-jd91k19z 97 37 modification modification NN cord-323428-jd91k19z 97 38 using use VBG cord-323428-jd91k19z 97 39 universal universal JJ cord-323428-jd91k19z 97 40 enzyme enzyme NN cord-323428-jd91k19z 97 41 conjugate conjugate NN cord-323428-jd91k19z 97 42 , , , cord-323428-jd91k19z 97 43 anti anti JJ cord-323428-jd91k19z 97 44 - - JJ cord-323428-jd91k19z 97 45 mouse mouse NN cord-323428-jd91k19z 97 46 IgGconjugated IgGconjugated NNP cord-323428-jd91k19z 97 47 HRP HRP NNP cord-323428-jd91k19z 97 48 , , , cord-323428-jd91k19z 97 49 to to TO cord-323428-jd91k19z 97 50 be be VB cord-323428-jd91k19z 97 51 able able JJ cord-323428-jd91k19z 97 52 to to TO cord-323428-jd91k19z 97 53 detect detect VB cord-323428-jd91k19z 97 54 mouse mouse NN cord-323428-jd91k19z 97 55 IgG IgG NNP cord-323428-jd91k19z 97 56 antibodies antibody NNS cord-323428-jd91k19z 97 57 instead instead RB cord-323428-jd91k19z 97 58 of of IN cord-323428-jd91k19z 97 59 human human JJ cord-323428-jd91k19z 97 60 IgG IgG NNP cord-323428-jd91k19z 97 61 antibodies antibody NNS cord-323428-jd91k19z 97 62 . . . cord-323428-jd91k19z 98 1 Microtiter microtiter JJ cord-323428-jd91k19z 98 2 plates plate NNS cord-323428-jd91k19z 98 3 were be VBD cord-323428-jd91k19z 98 4 pre pre JJ cord-323428-jd91k19z 98 5 - - JJ cord-323428-jd91k19z 98 6 coated coated JJ cord-323428-jd91k19z 98 7 with with IN cord-323428-jd91k19z 98 8 purified purify VBN cord-323428-jd91k19z 98 9 S1 s1 NN cord-323428-jd91k19z 98 10 antigen antigen NN cord-323428-jd91k19z 98 11 of of IN cord-323428-jd91k19z 98 12 MERS MERS NNP cord-323428-jd91k19z 98 13 - - HYPH cord-323428-jd91k19z 98 14 CoV. CoV. NNP cord-323428-jd91k19z 98 15 Mouse Mouse NNP cord-323428-jd91k19z 98 16 diluted diluted JJ cord-323428-jd91k19z 98 17 serum serum NN cord-323428-jd91k19z 98 18 samples sample NNS cord-323428-jd91k19z 98 19 ( ( -LRB- cord-323428-jd91k19z 98 20 1:100 1:100 CD cord-323428-jd91k19z 98 21 ) ) -RRB- cord-323428-jd91k19z 98 22 were be VBD cord-323428-jd91k19z 98 23 incubated incubate VBN cord-323428-jd91k19z 98 24 in in IN cord-323428-jd91k19z 98 25 each each DT cord-323428-jd91k19z 98 26 well well RB cord-323428-jd91k19z 98 27 from from IN cord-323428-jd91k19z 98 28 the the DT cord-323428-jd91k19z 98 29 three three CD cord-323428-jd91k19z 98 30 experimental experimental JJ cord-323428-jd91k19z 98 31 groups group NNS cord-323428-jd91k19z 98 32 ( ( -LRB- cord-323428-jd91k19z 98 33 Ad ad NN cord-323428-jd91k19z 98 34 - - HYPH cord-323428-jd91k19z 98 35 MERS MERS NNP cord-323428-jd91k19z 98 36 - - HYPH cord-323428-jd91k19z 98 37 S1 S1 NNP cord-323428-jd91k19z 98 38 , , , cord-323428-jd91k19z 98 39 Ad Ad NNP cord-323428-jd91k19z 98 40 - - HYPH cord-323428-jd91k19z 98 41 CMV CMV NNP cord-323428-jd91k19z 98 42 , , , cord-323428-jd91k19z 98 43 and and CC cord-323428-jd91k19z 98 44 PBS PBS NNP cord-323428-jd91k19z 98 45 ) ) -RRB- cord-323428-jd91k19z 98 46 for for IN cord-323428-jd91k19z 98 47 30 30 CD cord-323428-jd91k19z 98 48 min min NN cord-323428-jd91k19z 98 49 at at IN cord-323428-jd91k19z 98 50 room room NN cord-323428-jd91k19z 98 51 temperature temperature NN cord-323428-jd91k19z 98 52 by by IN cord-323428-jd91k19z 98 53 following follow VBG cord-323428-jd91k19z 98 54 the the DT cord-323428-jd91k19z 98 55 manufacturer manufacturer NN cord-323428-jd91k19z 98 56 's 's POS cord-323428-jd91k19z 98 57 instructions instruction NNS cord-323428-jd91k19z 98 58 . . . cord-323428-jd91k19z 99 1 The the DT cord-323428-jd91k19z 99 2 color color NN cord-323428-jd91k19z 99 3 intensity intensity NN cord-323428-jd91k19z 99 4 was be VBD cord-323428-jd91k19z 99 5 measured measure VBN cord-323428-jd91k19z 99 6 using use VBG cord-323428-jd91k19z 99 7 an an DT cord-323428-jd91k19z 99 8 ELISA ELISA NNP cord-323428-jd91k19z 99 9 plate plate NN cord-323428-jd91k19z 99 10 reader reader NN cord-323428-jd91k19z 99 11 ( ( -LRB- cord-323428-jd91k19z 99 12 BioTek BioTek NNP cord-323428-jd91k19z 99 13 , , , cord-323428-jd91k19z 99 14 Winooski Winooski NNP cord-323428-jd91k19z 99 15 , , , cord-323428-jd91k19z 99 16 VT VT NNP cord-323428-jd91k19z 99 17 , , , cord-323428-jd91k19z 99 18 USA USA NNP cord-323428-jd91k19z 99 19 ) ) -RRB- cord-323428-jd91k19z 99 20 . . . cord-323428-jd91k19z 100 1 Cell cell NN cord-323428-jd91k19z 100 2 culture culture NN cord-323428-jd91k19z 100 3 supernatants supernatant NNS cord-323428-jd91k19z 100 4 from from IN cord-323428-jd91k19z 100 5 stimulated stimulated JJ cord-323428-jd91k19z 100 6 and and CC cord-323428-jd91k19z 100 7 corresponding correspond VBG cord-323428-jd91k19z 100 8 non non JJ cord-323428-jd91k19z 100 9 - - JJ cord-323428-jd91k19z 100 10 stimulated stimulate VBN cord-323428-jd91k19z 100 11 samples sample NNS cord-323428-jd91k19z 100 12 of of IN cord-323428-jd91k19z 100 13 the the DT cord-323428-jd91k19z 100 14 three three CD cord-323428-jd91k19z 100 15 groups group NNS cord-323428-jd91k19z 100 16 were be VBD cord-323428-jd91k19z 100 17 tested test VBN cord-323428-jd91k19z 100 18 for for IN cord-323428-jd91k19z 100 19 the the DT cord-323428-jd91k19z 100 20 level level NN cord-323428-jd91k19z 100 21 of of IN cord-323428-jd91k19z 100 22 mouse mouse NN cord-323428-jd91k19z 100 23 cytokines cytokine NNS cord-323428-jd91k19z 100 24 at at IN cord-323428-jd91k19z 100 25 weeks week NNS cord-323428-jd91k19z 100 26 4 4 CD cord-323428-jd91k19z 100 27 and and CC cord-323428-jd91k19z 100 28 6 6 CD cord-323428-jd91k19z 100 29 post post JJ cord-323428-jd91k19z 100 30 - - JJ cord-323428-jd91k19z 100 31 prime prime JJ cord-323428-jd91k19z 100 32 using use VBG cord-323428-jd91k19z 100 33 a a DT cord-323428-jd91k19z 100 34 quantitative quantitative JJ cord-323428-jd91k19z 100 35 sandwich sandwich NN cord-323428-jd91k19z 100 36 enzyme enzyme NN cord-323428-jd91k19z 100 37 immunoassay immunoassay NN cord-323428-jd91k19z 100 38 technique technique NN cord-323428-jd91k19z 100 39 ( ( -LRB- cord-323428-jd91k19z 100 40 Quantikine Quantikine NNP cord-323428-jd91k19z 100 41 ELISA ELISA NNP cord-323428-jd91k19z 100 42 Mouse Mouse NNP cord-323428-jd91k19z 100 43 Cytokines Cytokines NNP cord-323428-jd91k19z 100 44 Immunoassay Immunoassay NNP cord-323428-jd91k19z 100 45 , , , cord-323428-jd91k19z 100 46 Minneapolis Minneapolis NNP cord-323428-jd91k19z 100 47 , , , cord-323428-jd91k19z 100 48 MN MN NNP cord-323428-jd91k19z 100 49 , , , cord-323428-jd91k19z 100 50 USA USA NNP cord-323428-jd91k19z 100 51 ) ) -RRB- cord-323428-jd91k19z 100 52 according accord VBG cord-323428-jd91k19z 100 53 to to IN cord-323428-jd91k19z 100 54 the the DT cord-323428-jd91k19z 100 55 manufacturer manufacturer NN cord-323428-jd91k19z 100 56 's 's POS cord-323428-jd91k19z 100 57 instructions instruction NNS cord-323428-jd91k19z 100 58 . . . cord-323428-jd91k19z 101 1 Relative relative JJ cord-323428-jd91k19z 101 2 quantification quantification NN cord-323428-jd91k19z 101 3 of of IN cord-323428-jd91k19z 101 4 mouse mouse NN cord-323428-jd91k19z 101 5 IFN IFN NNP cord-323428-jd91k19z 101 6 - - HYPH cord-323428-jd91k19z 101 7 γ γ NNP cord-323428-jd91k19z 101 8 and and CC cord-323428-jd91k19z 101 9 IL-4 IL-4 NNP cord-323428-jd91k19z 101 10 was be VBD cord-323428-jd91k19z 101 11 normalized normalize VBN cord-323428-jd91k19z 101 12 to to IN cord-323428-jd91k19z 101 13 the the DT cord-323428-jd91k19z 101 14 housekeeping housekeeping NN cord-323428-jd91k19z 101 15 gene gene NN cord-323428-jd91k19z 101 16 β β NNP cord-323428-jd91k19z 101 17 - - HYPH cord-323428-jd91k19z 101 18 actin actin NN cord-323428-jd91k19z 101 19 in in IN cord-323428-jd91k19z 101 20 the the DT cord-323428-jd91k19z 101 21 cell cell NN cord-323428-jd91k19z 101 22 culture culture NN cord-323428-jd91k19z 101 23 yield yield NN cord-323428-jd91k19z 101 24 of of IN cord-323428-jd91k19z 101 25 stimulated stimulate VBN cord-323428-jd91k19z 101 26 groups group NNS cord-323428-jd91k19z 101 27 . . . cord-323428-jd91k19z 102 1 The the DT cord-323428-jd91k19z 102 2 collected collect VBN cord-323428-jd91k19z 102 3 pellets pellet NNS cord-323428-jd91k19z 102 4 from from IN cord-323428-jd91k19z 102 5 cell cell NN cord-323428-jd91k19z 102 6 culture culture NN cord-323428-jd91k19z 102 7 were be VBD cord-323428-jd91k19z 102 8 used use VBN cord-323428-jd91k19z 102 9 to to TO cord-323428-jd91k19z 102 10 extract extract VB cord-323428-jd91k19z 102 11 total total JJ cord-323428-jd91k19z 102 12 RNA RNA NNP cord-323428-jd91k19z 102 13 using use VBG cord-323428-jd91k19z 102 14 the the DT cord-323428-jd91k19z 102 15 SV SV NNP cord-323428-jd91k19z 102 16 Total Total NNP cord-323428-jd91k19z 102 17 RNA RNA NNP cord-323428-jd91k19z 102 18 Isolation Isolation NNP cord-323428-jd91k19z 102 19 System System NNP cord-323428-jd91k19z 102 20 ( ( -LRB- cord-323428-jd91k19z 102 21 Promega Promega NNP cord-323428-jd91k19z 102 22 ) ) -RRB- cord-323428-jd91k19z 102 23 according accord VBG cord-323428-jd91k19z 102 24 to to IN cord-323428-jd91k19z 102 25 the the DT cord-323428-jd91k19z 102 26 manufacturer manufacturer NN cord-323428-jd91k19z 102 27 's 's POS cord-323428-jd91k19z 102 28 instructions instruction NNS cord-323428-jd91k19z 102 29 ; ; : cord-323428-jd91k19z 102 30 this this DT cord-323428-jd91k19z 102 31 extraction extraction NN cord-323428-jd91k19z 102 32 kit kit NN cord-323428-jd91k19z 102 33 has have VBZ cord-323428-jd91k19z 102 34 a a DT cord-323428-jd91k19z 102 35 DNase DNase NNP cord-323428-jd91k19z 102 36 incubation incubation NN cord-323428-jd91k19z 102 37 step step NN cord-323428-jd91k19z 102 38 to to TO cord-323428-jd91k19z 102 39 eliminate eliminate VB cord-323428-jd91k19z 102 40 any any DT cord-323428-jd91k19z 102 41 DNA dna NN cord-323428-jd91k19z 102 42 in in IN cord-323428-jd91k19z 102 43 the the DT cord-323428-jd91k19z 102 44 sample sample NN cord-323428-jd91k19z 102 45 . . . cord-323428-jd91k19z 103 1 Isolated isolated JJ cord-323428-jd91k19z 103 2 RNA RNA NNP cord-323428-jd91k19z 103 3 ( ( -LRB- cord-323428-jd91k19z 103 4 40 40 CD cord-323428-jd91k19z 103 5 µl µl IN cord-323428-jd91k19z 103 6 ) ) -RRB- cord-323428-jd91k19z 103 7 was be VBD cord-323428-jd91k19z 103 8 stored store VBN cord-323428-jd91k19z 103 9 at at IN cord-323428-jd91k19z 103 10 −80 −80 NNP cord-323428-jd91k19z 103 11 ° ° NN cord-323428-jd91k19z 103 12 C C NNP cord-323428-jd91k19z 103 13 before before IN cord-323428-jd91k19z 103 14 RT RT NNP cord-323428-jd91k19z 103 15 - - HYPH cord-323428-jd91k19z 103 16 PCR PCR NNP cord-323428-jd91k19z 103 17 analysis analysis NN cord-323428-jd91k19z 103 18 . . . cord-323428-jd91k19z 104 1 RNA RNA NNP cord-323428-jd91k19z 104 2 concentration concentration NN cord-323428-jd91k19z 104 3 was be VBD cord-323428-jd91k19z 104 4 adjusted adjust VBN cord-323428-jd91k19z 104 5 to to IN cord-323428-jd91k19z 104 6 10 10 CD cord-323428-jd91k19z 104 7 ng/µl ng/µl CD cord-323428-jd91k19z 104 8 using use VBG cord-323428-jd91k19z 104 9 TE TE NNP cord-323428-jd91k19z 104 10 buffer buffer NN cord-323428-jd91k19z 104 11 . . . cord-323428-jd91k19z 105 1 Purified purify VBN cord-323428-jd91k19z 105 2 RNA RNA NNP cord-323428-jd91k19z 105 3 ( ( -LRB- cord-323428-jd91k19z 105 4 10 10 CD cord-323428-jd91k19z 105 5 µl µl IN cord-323428-jd91k19z 105 6 ) ) -RRB- cord-323428-jd91k19z 105 7 was be VBD cord-323428-jd91k19z 105 8 used use VBN cord-323428-jd91k19z 105 9 as as IN cord-323428-jd91k19z 105 10 a a DT cord-323428-jd91k19z 105 11 template template NN cord-323428-jd91k19z 105 12 to to TO cord-323428-jd91k19z 105 13 produce produce VB cord-323428-jd91k19z 105 14 20 20 CD cord-323428-jd91k19z 105 15 µl µl IN cord-323428-jd91k19z 105 16 of of IN cord-323428-jd91k19z 105 17 cDNA cdna NN cord-323428-jd91k19z 105 18 using use VBG cord-323428-jd91k19z 105 19 the the DT cord-323428-jd91k19z 105 20 reverse reverse JJ cord-323428-jd91k19z 105 21 transcription transcription NN cord-323428-jd91k19z 105 22 system system NN cord-323428-jd91k19z 105 23 ( ( -LRB- cord-323428-jd91k19z 105 24 Promega Promega NNP cord-323428-jd91k19z 105 25 ) ) -RRB- cord-323428-jd91k19z 105 26 kit kit NN cord-323428-jd91k19z 105 27 . . . cord-323428-jd91k19z 106 1 Relative relative JJ cord-323428-jd91k19z 106 2 RT RT NNP cord-323428-jd91k19z 106 3 - - HYPH cord-323428-jd91k19z 106 4 PCR PCR NNP cord-323428-jd91k19z 106 5 assay assay NN cord-323428-jd91k19z 106 6 was be VBD cord-323428-jd91k19z 106 7 performed perform VBN cord-323428-jd91k19z 106 8 to to TO cord-323428-jd91k19z 106 9 determine determine VB cord-323428-jd91k19z 106 10 the the DT cord-323428-jd91k19z 106 11 mRNA mRNA NNP cord-323428-jd91k19z 106 12 expression expression NN cord-323428-jd91k19z 106 13 levels level NNS cord-323428-jd91k19z 106 14 of of IN cord-323428-jd91k19z 106 15 mouse mouse NN cord-323428-jd91k19z 106 16 IFN IFN NNP cord-323428-jd91k19z 106 17 - - HYPH cord-323428-jd91k19z 106 18 γ γ NNP cord-323428-jd91k19z 106 19 and and CC cord-323428-jd91k19z 106 20 IL-4 IL-4 NNP cord-323428-jd91k19z 106 21 in in IN cord-323428-jd91k19z 106 22 the the DT cord-323428-jd91k19z 106 23 cell cell NN cord-323428-jd91k19z 106 24 culture culture NN cord-323428-jd91k19z 106 25 . . . cord-323428-jd91k19z 107 1 The the DT cord-323428-jd91k19z 107 2 fold fold JJ cord-323428-jd91k19z 107 3 changes change NNS cord-323428-jd91k19z 107 4 were be VBD cord-323428-jd91k19z 107 5 calculated calculate VBN cord-323428-jd91k19z 107 6 using use VBG cord-323428-jd91k19z 107 7 the the DT cord-323428-jd91k19z 107 8 2 2 CD cord-323428-jd91k19z 107 9 −ΔΔCT −ΔΔCT NNP cord-323428-jd91k19z 107 10 method method NN cord-323428-jd91k19z 107 11 normalized normalize VBN cord-323428-jd91k19z 107 12 to to IN cord-323428-jd91k19z 107 13 β β NN cord-323428-jd91k19z 107 14 - - HYPH cord-323428-jd91k19z 107 15 actin actin VBG cord-323428-jd91k19z 107 16 [ [ -LRB- cord-323428-jd91k19z 107 17 34 34 CD cord-323428-jd91k19z 107 18 ] ] -RRB- cord-323428-jd91k19z 107 19 . . . cord-323428-jd91k19z 108 1 Specific specific JJ cord-323428-jd91k19z 108 2 primer primer NN cord-323428-jd91k19z 108 3 sets set NNS cord-323428-jd91k19z 108 4 were be VBD cord-323428-jd91k19z 108 5 used use VBN cord-323428-jd91k19z 108 6 , , , cord-323428-jd91k19z 108 7 as as IN cord-323428-jd91k19z 108 8 shown show VBN cord-323428-jd91k19z 108 9 in in IN cord-323428-jd91k19z 108 10 Table- Table- NNP cord-323428-jd91k19z 108 11 2 2 CD cord-323428-jd91k19z 108 12 [ [ -LRB- cord-323428-jd91k19z 108 13 35 35 CD cord-323428-jd91k19z 108 14 ] ] -RRB- cord-323428-jd91k19z 108 15 . . . cord-323428-jd91k19z 109 1 Extracted extract VBN cord-323428-jd91k19z 109 2 RNA RNA NNP cord-323428-jd91k19z 109 3 ( ( -LRB- cord-323428-jd91k19z 109 4 2 2 CD cord-323428-jd91k19z 109 5 µl µl IN cord-323428-jd91k19z 109 6 ) ) -RRB- cord-323428-jd91k19z 109 7 was be VBD cord-323428-jd91k19z 109 8 used use VBN cord-323428-jd91k19z 109 9 as as IN cord-323428-jd91k19z 109 10 a a DT cord-323428-jd91k19z 109 11 template template NN cord-323428-jd91k19z 109 12 in in IN cord-323428-jd91k19z 109 13 the the DT cord-323428-jd91k19z 109 14 PCR PCR NNP cord-323428-jd91k19z 109 15 reaction reaction NN cord-323428-jd91k19z 109 16 mixture mixture NN cord-323428-jd91k19z 109 17 ( ( -LRB- cord-323428-jd91k19z 109 18 20 20 CD cord-323428-jd91k19z 109 19 µl µl IN cord-323428-jd91k19z 109 20 ) ) -RRB- cord-323428-jd91k19z 109 21 , , , cord-323428-jd91k19z 109 22 which which WDT cord-323428-jd91k19z 109 23 was be VBD cord-323428-jd91k19z 109 24 composed compose VBN cord-323428-jd91k19z 109 25 of of IN cord-323428-jd91k19z 109 26 0.5 0.5 CD cord-323428-jd91k19z 109 27 µM µM . cord-323428-jd91k19z 110 1 each each DT cord-323428-jd91k19z 110 2 of of IN cord-323428-jd91k19z 110 3 the the DT cord-323428-jd91k19z 110 4 forward forward NN cord-323428-jd91k19z 110 5 and and CC cord-323428-jd91k19z 110 6 reverse reverse VB cord-323428-jd91k19z 110 7 primers primer NNS cord-323428-jd91k19z 110 8 , , , cord-323428-jd91k19z 110 9 10 10 CD cord-323428-jd91k19z 110 10 µl µl IN cord-323428-jd91k19z 110 11 of of IN cord-323428-jd91k19z 110 12 PowerUp PowerUp NNP cord-323428-jd91k19z 110 13 SYPR SYPR NNP cord-323428-jd91k19z 110 14 Green Green NNP cord-323428-jd91k19z 110 15 Master Master NNP cord-323428-jd91k19z 110 16 Mix Mix NNP cord-323428-jd91k19z 110 17 ( ( -LRB- cord-323428-jd91k19z 110 18 Applied Applied NNP cord-323428-jd91k19z 110 19 Biosystems Biosystems NNP cord-323428-jd91k19z 110 20 , , , cord-323428-jd91k19z 110 21 Foster Foster NNP cord-323428-jd91k19z 110 22 City City NNP cord-323428-jd91k19z 110 23 , , , cord-323428-jd91k19z 110 24 CA CA NNP cord-323428-jd91k19z 110 25 , , , cord-323428-jd91k19z 110 26 USA USA NNP cord-323428-jd91k19z 110 27 ) ) -RRB- cord-323428-jd91k19z 110 28 , , , cord-323428-jd91k19z 110 29 and and CC cord-323428-jd91k19z 110 30 6 6 CD cord-323428-jd91k19z 110 31 µl µl IN cord-323428-jd91k19z 110 32 of of IN cord-323428-jd91k19z 110 33 nuclease nuclease NN cord-323428-jd91k19z 110 34 - - HYPH cord-323428-jd91k19z 110 35 free free JJ cord-323428-jd91k19z 110 36 water water NN cord-323428-jd91k19z 110 37 . . . cord-323428-jd91k19z 111 1 RT RT NNP cord-323428-jd91k19z 111 2 - - HYPH cord-323428-jd91k19z 111 3 PCR PCR NNP cord-323428-jd91k19z 111 4 conditions condition NNS cord-323428-jd91k19z 111 5 were be VBD cord-323428-jd91k19z 111 6 programmed program VBN cord-323428-jd91k19z 111 7 in in IN cord-323428-jd91k19z 111 8 Rotor Rotor NNP cord-323428-jd91k19z 111 9 Gene Gene NNP cord-323428-jd91k19z 111 10 - - HYPH cord-323428-jd91k19z 111 11 Q Q NNP cord-323428-jd91k19z 111 12 ( ( -LRB- cord-323428-jd91k19z 111 13 Qiagen Qiagen NNP cord-323428-jd91k19z 111 14 , , , cord-323428-jd91k19z 111 15 Hilden Hilden NNP cord-323428-jd91k19z 111 16 , , , cord-323428-jd91k19z 111 17 Germany Germany NNP cord-323428-jd91k19z 111 18 ) ) -RRB- cord-323428-jd91k19z 111 19 as as IN cord-323428-jd91k19z 111 20 follows follow VBZ cord-323428-jd91k19z 111 21 : : : cord-323428-jd91k19z 112 1 50 50 CD cord-323428-jd91k19z 112 2 ° ° NN cord-323428-jd91k19z 112 3 C c NN cord-323428-jd91k19z 112 4 for for IN cord-323428-jd91k19z 112 5 2 2 CD cord-323428-jd91k19z 112 6 min min NN cord-323428-jd91k19z 112 7 , , , cord-323428-jd91k19z 112 8 95 95 CD cord-323428-jd91k19z 112 9 ° ° NN cord-323428-jd91k19z 112 10 C c NN cord-323428-jd91k19z 112 11 for for IN cord-323428-jd91k19z 112 12 10 10 CD cord-323428-jd91k19z 112 13 min min NN cord-323428-jd91k19z 112 14 , , , cord-323428-jd91k19z 112 15 followed follow VBN cord-323428-jd91k19z 112 16 by by IN cord-323428-jd91k19z 112 17 40 40 CD cord-323428-jd91k19z 112 18 cycles cycle NNS cord-323428-jd91k19z 112 19 of of IN cord-323428-jd91k19z 112 20 95 95 CD cord-323428-jd91k19z 112 21 ° ° NN cord-323428-jd91k19z 112 22 C C NNP cord-323428-jd91k19z 112 23 s s NNP cord-323428-jd91k19z 112 24 , , , cord-323428-jd91k19z 112 25 60 60 CD cord-323428-jd91k19z 112 26 ° ° NN cord-323428-jd91k19z 112 27 C c NN cord-323428-jd91k19z 112 28 for for IN cord-323428-jd91k19z 112 29 1 1 CD cord-323428-jd91k19z 112 30 min min NN cord-323428-jd91k19z 112 31 , , , cord-323428-jd91k19z 112 32 and and CC cord-323428-jd91k19z 112 33 a a DT cord-323428-jd91k19z 112 34 melting melting NN cord-323428-jd91k19z 112 35 step step NN cord-323428-jd91k19z 112 36 at at IN cord-323428-jd91k19z 112 37 55 55 CD cord-323428-jd91k19z 112 38 - - SYM cord-323428-jd91k19z 112 39 99 99 CD cord-323428-jd91k19z 112 40 ° ° NN cord-323428-jd91k19z 112 41 C c NN cord-323428-jd91k19z 112 42 for for IN cord-323428-jd91k19z 112 43 30 30 CD cord-323428-jd91k19z 112 44 s. s. NNP cord-323428-jd91k19z 112 45 Data Data NNP cord-323428-jd91k19z 112 46 for for IN cord-323428-jd91k19z 112 47 ELISA ELISA NNP cord-323428-jd91k19z 112 48 and and CC cord-323428-jd91k19z 112 49 fold fold VB cord-323428-jd91k19z 112 50 changes change NNS cord-323428-jd91k19z 112 51 were be VBD cord-323428-jd91k19z 112 52 expressed express VBN cord-323428-jd91k19z 112 53 at at IN cord-323428-jd91k19z 112 54 mean mean NN cord-323428-jd91k19z 112 55 ± ± NNP cord-323428-jd91k19z 112 56 standard standard JJ cord-323428-jd91k19z 112 57 deviation deviation NN cord-323428-jd91k19z 112 58 . . . cord-323428-jd91k19z 113 1 One one CD cord-323428-jd91k19z 113 2 - - HYPH cord-323428-jd91k19z 113 3 way way NN cord-323428-jd91k19z 113 4 ANOVA ANOVA NNP cord-323428-jd91k19z 113 5 and and CC cord-323428-jd91k19z 113 6 t t NN cord-323428-jd91k19z 113 7 - - HYPH cord-323428-jd91k19z 113 8 test test NN cord-323428-jd91k19z 113 9 were be VBD cord-323428-jd91k19z 113 10 used use VBN cord-323428-jd91k19z 113 11 to to TO cord-323428-jd91k19z 113 12 compare compare VB cord-323428-jd91k19z 113 13 different different JJ cord-323428-jd91k19z 113 14 values value NNS cord-323428-jd91k19z 113 15 in in IN cord-323428-jd91k19z 113 16 all all DT cord-323428-jd91k19z 113 17 groups group NNS cord-323428-jd91k19z 113 18 using use VBG cord-323428-jd91k19z 113 19 OpenEpi OpenEpi NNP cord-323428-jd91k19z 113 20 software software NN cord-323428-jd91k19z 113 21 ( ( -LRB- cord-323428-jd91k19z 113 22 Emory Emory NNP cord-323428-jd91k19z 113 23 University University NNP cord-323428-jd91k19z 113 24 , , , cord-323428-jd91k19z 113 25 USA USA NNP cord-323428-jd91k19z 113 26 ) ) -RRB- cord-323428-jd91k19z 113 27 . . . cord-323428-jd91k19z 114 1 Parameter parameter NN cord-323428-jd91k19z 114 2 differences difference NNS cord-323428-jd91k19z 114 3 were be VBD cord-323428-jd91k19z 114 4 considered consider VBN cord-323428-jd91k19z 114 5 statistically statistically RB cord-323428-jd91k19z 114 6 significant significant JJ cord-323428-jd91k19z 114 7 at at IN cord-323428-jd91k19z 114 8 p<0.05 p<0.05 CD cord-323428-jd91k19z 114 9 . . . cord-323428-jd91k19z 115 1 Parameter parameter NN cord-323428-jd91k19z 115 2 differences difference NNS cord-323428-jd91k19z 115 3 were be VBD cord-323428-jd91k19z 115 4 indicated indicate VBN cord-323428-jd91k19z 115 5 by by IN cord-323428-jd91k19z 115 6 small small JJ cord-323428-jd91k19z 115 7 letters letter NNS cord-323428-jd91k19z 115 8 labeled label VBN cord-323428-jd91k19z 115 9 within within IN cord-323428-jd91k19z 115 10 each each DT cord-323428-jd91k19z 115 11 group group NN cord-323428-jd91k19z 115 12 ; ; : cord-323428-jd91k19z 115 13 different different JJ cord-323428-jd91k19z 115 14 letters letter NNS cord-323428-jd91k19z 115 15 indicated indicate VBD cord-323428-jd91k19z 115 16 significant significant JJ cord-323428-jd91k19z 115 17 differences difference NNS cord-323428-jd91k19z 115 18 at at IN cord-323428-jd91k19z 115 19 p<0.05 p<0.05 CD cord-323428-jd91k19z 115 20 . . . cord-323428-jd91k19z 116 1 Distribution distribution NN cord-323428-jd91k19z 116 2 and and CC cord-323428-jd91k19z 116 3 expression expression NN cord-323428-jd91k19z 116 4 of of IN cord-323428-jd91k19z 116 5 the the DT cord-323428-jd91k19z 116 6 S1 S1 NNP cord-323428-jd91k19z 116 7 subunit subunit NN cord-323428-jd91k19z 116 8 of of IN cord-323428-jd91k19z 116 9 the the DT cord-323428-jd91k19z 116 10 MERS MERS NNP cord-323428-jd91k19z 116 11 - - HYPH cord-323428-jd91k19z 116 12 CoV CoV NNP cord-323428-jd91k19z 116 13 spike spike NN cord-323428-jd91k19z 116 14 protein protein NN cord-323428-jd91k19z 116 15 in in IN cord-323428-jd91k19z 116 16 mice mice NN cord-323428-jd91k19z 116 17 tissues tissue NNS cord-323428-jd91k19z 116 18 were be VBD cord-323428-jd91k19z 116 19 detected detect VBN cord-323428-jd91k19z 116 20 at at IN cord-323428-jd91k19z 116 21 weeks week NNS cord-323428-jd91k19z 116 22 3 3 CD cord-323428-jd91k19z 116 23 and and CC cord-323428-jd91k19z 116 24 5 5 CD cord-323428-jd91k19z 116 25 post post JJ cord-323428-jd91k19z 116 26 first first JJ cord-323428-jd91k19z 116 27 immunization immunization NN cord-323428-jd91k19z 116 28 in in IN cord-323428-jd91k19z 116 29 the the DT cord-323428-jd91k19z 116 30 kidneys kidney NNS cord-323428-jd91k19z 116 31 and and CC cord-323428-jd91k19z 116 32 lungs lung NNS cord-323428-jd91k19z 116 33 of of IN cord-323428-jd91k19z 116 34 the the DT cord-323428-jd91k19z 116 35 vaccinated vaccinated JJ cord-323428-jd91k19z 116 36 group group NN cord-323428-jd91k19z 116 37 but but CC cord-323428-jd91k19z 116 38 not not RB cord-323428-jd91k19z 116 39 in in IN cord-323428-jd91k19z 116 40 control control NN cord-323428-jd91k19z 116 41 groups group NNS cord-323428-jd91k19z 116 42 using use VBG cord-323428-jd91k19z 116 43 conventional conventional JJ cord-323428-jd91k19z 116 44 PCR PCR NNP cord-323428-jd91k19z 116 45 ( ( -LRB- cord-323428-jd91k19z 116 46 Figure-1 Figure-1 NNP cord-323428-jd91k19z 116 47 ) ) -RRB- cord-323428-jd91k19z 116 48 . . . cord-323428-jd91k19z 117 1 Using use VBG cord-323428-jd91k19z 117 2 IHC IHC NNP cord-323428-jd91k19z 117 3 , , , cord-323428-jd91k19z 117 4 S1 s1 NN cord-323428-jd91k19z 117 5 protein protein NN cord-323428-jd91k19z 117 6 expression expression NN cord-323428-jd91k19z 117 7 was be VBD cord-323428-jd91k19z 117 8 detected detect VBN cord-323428-jd91k19z 117 9 in in IN cord-323428-jd91k19z 117 10 the the DT cord-323428-jd91k19z 117 11 cytoplasm cytoplasm NN cord-323428-jd91k19z 117 12 of of IN cord-323428-jd91k19z 117 13 proximal proximal JJ cord-323428-jd91k19z 117 14 tubule tubule NN cord-323428-jd91k19z 117 15 epithelium epithelium NN cord-323428-jd91k19z 117 16 in in IN cord-323428-jd91k19z 117 17 the the DT cord-323428-jd91k19z 117 18 vaccinated vaccinated JJ cord-323428-jd91k19z 117 19 mice mouse NNS cord-323428-jd91k19z 117 20 at at IN cord-323428-jd91k19z 117 21 week week NN cord-323428-jd91k19z 117 22 6 6 CD cord-323428-jd91k19z 117 23 post post VBP cord-323428-jd91k19z 117 24 first first JJ cord-323428-jd91k19z 117 25 immunization immunization NN cord-323428-jd91k19z 117 26 but but CC cord-323428-jd91k19z 117 27 not not RB cord-323428-jd91k19z 117 28 in in IN cord-323428-jd91k19z 117 29 the the DT cord-323428-jd91k19z 117 30 control control NN cord-323428-jd91k19z 117 31 groups group NNS cord-323428-jd91k19z 117 32 ( ( -LRB- cord-323428-jd91k19z 117 33 Figure-2 Figure-2 NNP cord-323428-jd91k19z 117 34 ) ) -RRB- cord-323428-jd91k19z 117 35 . . . cord-323428-jd91k19z 118 1 S1 s1 NN cord-323428-jd91k19z 118 2 expression expression NN cord-323428-jd91k19z 118 3 was be VBD cord-323428-jd91k19z 118 4 not not RB cord-323428-jd91k19z 118 5 detected detect VBN cord-323428-jd91k19z 118 6 in in IN cord-323428-jd91k19z 118 7 lung lung NN cord-323428-jd91k19z 118 8 tissues tissue NNS cord-323428-jd91k19z 118 9 in in IN cord-323428-jd91k19z 118 10 either either CC cord-323428-jd91k19z 118 11 the the DT cord-323428-jd91k19z 118 12 vaccinated vaccinated JJ cord-323428-jd91k19z 118 13 or or CC cord-323428-jd91k19z 118 14 the the DT cord-323428-jd91k19z 118 15 control control NN cord-323428-jd91k19z 118 16 groups group NNS cord-323428-jd91k19z 118 17 ( ( -LRB- cord-323428-jd91k19z 118 18 Figure-2 Figure-2 NNP cord-323428-jd91k19z 118 19 ) ) -RRB- cord-323428-jd91k19z 118 20 . . . cord-323428-jd91k19z 119 1 There there EX cord-323428-jd91k19z 119 2 was be VBD cord-323428-jd91k19z 119 3 a a DT cord-323428-jd91k19z 119 4 significant significant JJ cord-323428-jd91k19z 119 5 level level NN cord-323428-jd91k19z 119 6 ( ( -LRB- cord-323428-jd91k19z 119 7 p<0.05 p<0.05 CD cord-323428-jd91k19z 119 8 ) ) -RRB- cord-323428-jd91k19z 119 9 of of IN cord-323428-jd91k19z 119 10 MERS MERS NNP cord-323428-jd91k19z 119 11 - - HYPH cord-323428-jd91k19z 119 12 S1 S1 NNP cord-323428-jd91k19z 119 13 specific specific JJ cord-323428-jd91k19z 119 14 IgG igg NN cord-323428-jd91k19z 119 15 antibodies antibody NNS cord-323428-jd91k19z 119 16 detected detect VBN cord-323428-jd91k19z 119 17 in in IN cord-323428-jd91k19z 119 18 the the DT cord-323428-jd91k19z 119 19 mice mice NN cord-323428-jd91k19z 119 20 sera sera NNP cord-323428-jd91k19z 119 21 of of IN cord-323428-jd91k19z 119 22 the the DT cord-323428-jd91k19z 119 23 vaccinated vaccinated JJ cord-323428-jd91k19z 119 24 group group NN cord-323428-jd91k19z 119 25 at at IN cord-323428-jd91k19z 119 26 weeks week NNS cord-323428-jd91k19z 119 27 4 4 CD cord-323428-jd91k19z 119 28 and and CC cord-323428-jd91k19z 119 29 6 6 CD cord-323428-jd91k19z 119 30 post post JJ cord-323428-jd91k19z 119 31 - - JJ cord-323428-jd91k19z 119 32 prime prime JJ cord-323428-jd91k19z 119 33 . . . cord-323428-jd91k19z 120 1 The the DT cord-323428-jd91k19z 120 2 combined combined JJ cord-323428-jd91k19z 120 3 results result NNS cord-323428-jd91k19z 120 4 of of IN cord-323428-jd91k19z 120 5 weeks week NNS cord-323428-jd91k19z 120 6 4 4 CD cord-323428-jd91k19z 120 7 and and CC cord-323428-jd91k19z 120 8 6 6 CD cord-323428-jd91k19z 120 9 revealed reveal VBD cord-323428-jd91k19z 120 10 that that IN cord-323428-jd91k19z 120 11 the the DT cord-323428-jd91k19z 120 12 IgG IgG NNP cord-323428-jd91k19z 120 13 antibody antibody NN cord-323428-jd91k19z 120 14 response response NN cord-323428-jd91k19z 120 15 was be VBD cord-323428-jd91k19z 120 16 significantly significantly RB cord-323428-jd91k19z 120 17 higher high JJR cord-323428-jd91k19z 120 18 ( ( -LRB- cord-323428-jd91k19z 120 19 p<0.05 p<0.05 CD cord-323428-jd91k19z 120 20 ) ) -RRB- cord-323428-jd91k19z 121 1 in in IN cord-323428-jd91k19z 121 2 the the DT cord-323428-jd91k19z 121 3 vaccinated vaccinated JJ cord-323428-jd91k19z 121 4 group group NN cord-323428-jd91k19z 121 5 than than IN cord-323428-jd91k19z 121 6 in in IN cord-323428-jd91k19z 121 7 the the DT cord-323428-jd91k19z 121 8 control control NN cord-323428-jd91k19z 121 9 groups group NNS cord-323428-jd91k19z 121 10 ( ( -LRB- cord-323428-jd91k19z 121 11 Figure-3 Figure-3 NNP cord-323428-jd91k19z 121 12 ) ) -RRB- cord-323428-jd91k19z 121 13 . . . cord-323428-jd91k19z 122 1 Available available JJ cord-323428-jd91k19z 122 2 at at IN cord-323428-jd91k19z 122 3 www.veterinaryworld.org/Vol.12/October-2019/7.pdf www.veterinaryworld.org/Vol.12/October-2019/7.pdf NNP cord-323428-jd91k19z 123 1 The the DT cord-323428-jd91k19z 123 2 level level NN cord-323428-jd91k19z 123 3 of of IN cord-323428-jd91k19z 123 4 mouse mouse NN cord-323428-jd91k19z 123 5 cytokines cytokine NNS cord-323428-jd91k19z 123 6 at at IN cord-323428-jd91k19z 123 7 weeks week NNS cord-323428-jd91k19z 123 8 4 4 CD cord-323428-jd91k19z 123 9 and and CC cord-323428-jd91k19z 123 10 6 6 CD cord-323428-jd91k19z 123 11 after after IN cord-323428-jd91k19z 123 12 the the DT cord-323428-jd91k19z 123 13 first first JJ cord-323428-jd91k19z 123 14 vaccination vaccination NN cord-323428-jd91k19z 123 15 in in IN cord-323428-jd91k19z 123 16 the the DT cord-323428-jd91k19z 123 17 cell cell NN cord-323428-jd91k19z 123 18 culture culture NN cord-323428-jd91k19z 123 19 supernatants supernatant NNS cord-323428-jd91k19z 123 20 of of IN cord-323428-jd91k19z 123 21 stimulated stimulate VBN cord-323428-jd91k19z 123 22 and and CC cord-323428-jd91k19z 123 23 corresponding correspond VBG cord-323428-jd91k19z 123 24 non non JJ cord-323428-jd91k19z 123 25 - - JJ cord-323428-jd91k19z 123 26 stimulated stimulate VBN cord-323428-jd91k19z 123 27 samples sample NNS cord-323428-jd91k19z 123 28 of of IN cord-323428-jd91k19z 123 29 the the DT cord-323428-jd91k19z 123 30 three three CD cord-323428-jd91k19z 123 31 groups group NNS cord-323428-jd91k19z 123 32 was be VBD cord-323428-jd91k19z 123 33 tested test VBN cord-323428-jd91k19z 123 34 by by IN cord-323428-jd91k19z 123 35 sandwich sandwich NN cord-323428-jd91k19z 123 36 ELISA ELISA NNP cord-323428-jd91k19z 123 37 assay assay NN cord-323428-jd91k19z 123 38 . . . cord-323428-jd91k19z 124 1 Mice mice NN cord-323428-jd91k19z 124 2 cytokines cytokine NNS cord-323428-jd91k19z 124 3 ( ( -LRB- cord-323428-jd91k19z 124 4 IFN IFN NNP cord-323428-jd91k19z 124 5 - - HYPH cord-323428-jd91k19z 124 6 γ γ NNP cord-323428-jd91k19z 124 7 , , , cord-323428-jd91k19z 124 8 IL-12 IL-12 NNP cord-323428-jd91k19z 124 9 , , , cord-323428-jd91k19z 124 10 and and CC cord-323428-jd91k19z 124 11 IL-4 IL-4 NNP cord-323428-jd91k19z 124 12 ) ) -RRB- cord-323428-jd91k19z 124 13 production production NN cord-323428-jd91k19z 124 14 in in IN cord-323428-jd91k19z 124 15 the the DT cord-323428-jd91k19z 124 16 Ad Ad NNP cord-323428-jd91k19z 124 17 - - HYPH cord-323428-jd91k19z 124 18 CMV CMV NNP cord-323428-jd91k19z 124 19 and and CC cord-323428-jd91k19z 124 20 PBS PBS NNP cord-323428-jd91k19z 124 21 groups group NNS cord-323428-jd91k19z 124 22 were be VBD cord-323428-jd91k19z 124 23 combined combine VBN cord-323428-jd91k19z 124 24 and and CC cord-323428-jd91k19z 124 25 expressed express VBN cord-323428-jd91k19z 124 26 collectively collectively RB cord-323428-jd91k19z 124 27 as as IN cord-323428-jd91k19z 124 28 one one CD cord-323428-jd91k19z 124 29 group group NN cord-323428-jd91k19z 124 30 ( ( -LRB- cord-323428-jd91k19z 124 31 control control NN cord-323428-jd91k19z 124 32 groups group NNS cord-323428-jd91k19z 124 33 ) ) -RRB- cord-323428-jd91k19z 124 34 due due IN cord-323428-jd91k19z 124 35 to to IN cord-323428-jd91k19z 124 36 the the DT cord-323428-jd91k19z 124 37 non non JJ cord-323428-jd91k19z 124 38 - - JJ cord-323428-jd91k19z 124 39 significant significant JJ cord-323428-jd91k19z 124 40 differences difference NNS cord-323428-jd91k19z 124 41 shown show VBN cord-323428-jd91k19z 124 42 across across IN cord-323428-jd91k19z 124 43 these these DT cord-323428-jd91k19z 124 44 time time NN cord-323428-jd91k19z 124 45 points point NNS cord-323428-jd91k19z 124 46 between between IN cord-323428-jd91k19z 124 47 these these DT cord-323428-jd91k19z 124 48 two two CD cord-323428-jd91k19z 124 49 groups group NNS cord-323428-jd91k19z 124 50 ( ( -LRB- cord-323428-jd91k19z 124 51 Figure-4 Figure-4 NNP cord-323428-jd91k19z 124 52 ) ) -RRB- cord-323428-jd91k19z 124 53 . . . cord-323428-jd91k19z 125 1 Therefore therefore RB cord-323428-jd91k19z 125 2 , , , cord-323428-jd91k19z 125 3 the the DT cord-323428-jd91k19z 125 4 levels level NNS cord-323428-jd91k19z 125 5 of of IN cord-323428-jd91k19z 125 6 each each DT cord-323428-jd91k19z 125 7 cytokine cytokine NN cord-323428-jd91k19z 125 8 at at IN cord-323428-jd91k19z 125 9 the the DT cord-323428-jd91k19z 125 10 two two CD cord-323428-jd91k19z 125 11 tested test VBN cord-323428-jd91k19z 125 12 time time NN cord-323428-jd91k19z 125 13 points point NNS cord-323428-jd91k19z 125 14 ( ( -LRB- cord-323428-jd91k19z 125 15 weeks week NNS cord-323428-jd91k19z 125 16 4 4 CD cord-323428-jd91k19z 125 17 and and CC cord-323428-jd91k19z 125 18 6 6 CD cord-323428-jd91k19z 125 19 after after IN cord-323428-jd91k19z 125 20 the the DT cord-323428-jd91k19z 125 21 first first JJ cord-323428-jd91k19z 125 22 vaccination vaccination NN cord-323428-jd91k19z 125 23 ) ) -RRB- cord-323428-jd91k19z 125 24 were be VBD cord-323428-jd91k19z 125 25 also also RB cord-323428-jd91k19z 125 26 combined combine VBN cord-323428-jd91k19z 125 27 and and CC cord-323428-jd91k19z 125 28 expressed express VBD cord-323428-jd91k19z 125 29 as as IN cord-323428-jd91k19z 125 30 one one CD cord-323428-jd91k19z 125 31 unit unit NN cord-323428-jd91k19z 125 32 . . . cord-323428-jd91k19z 126 1 Regardless regardless RB cord-323428-jd91k19z 126 2 of of IN cord-323428-jd91k19z 126 3 in in IN cord-323428-jd91k19z 126 4 vitro vitro FW cord-323428-jd91k19z 126 5 stimulation stimulation NN cord-323428-jd91k19z 126 6 , , , cord-323428-jd91k19z 126 7 production production NN cord-323428-jd91k19z 126 8 of of IN cord-323428-jd91k19z 126 9 IFN IFN NNP cord-323428-jd91k19z 126 10 - - HYPH cord-323428-jd91k19z 126 11 γ γ NNP cord-323428-jd91k19z 126 12 was be VBD cord-323428-jd91k19z 126 13 significantly significantly RB cord-323428-jd91k19z 126 14 ( ( -LRB- cord-323428-jd91k19z 126 15 p<0.05 p<0.05 CD cord-323428-jd91k19z 126 16 ) ) -RRB- cord-323428-jd91k19z 126 17 higher high JJR cord-323428-jd91k19z 126 18 in in IN cord-323428-jd91k19z 126 19 the the DT cord-323428-jd91k19z 126 20 vaccinated vaccinated JJ cord-323428-jd91k19z 126 21 group group NN cord-323428-jd91k19z 126 22 compared compare VBN cord-323428-jd91k19z 126 23 with with IN cord-323428-jd91k19z 126 24 the the DT cord-323428-jd91k19z 126 25 control control NN cord-323428-jd91k19z 126 26 groups group NNS cord-323428-jd91k19z 126 27 ( ( -LRB- cord-323428-jd91k19z 126 28 Ad Ad NNP cord-323428-jd91k19z 126 29 - - HYPH cord-323428-jd91k19z 126 30 CMV CMV NNP cord-323428-jd91k19z 126 31 and and CC cord-323428-jd91k19z 126 32 PBS PBS NNP cord-323428-jd91k19z 126 33 ) ) -RRB- cord-323428-jd91k19z 126 34 at at RB cord-323428-jd91k19z 126 35 all all RB cord-323428-jd91k19z 126 36 tested test VBN cord-323428-jd91k19z 126 37 time time NN cord-323428-jd91k19z 126 38 points point NNS cord-323428-jd91k19z 126 39 ( ( -LRB- cord-323428-jd91k19z 126 40 Figure-4 Figure-4 NNP cord-323428-jd91k19z 126 41 ) ) -RRB- cord-323428-jd91k19z 126 42 . . . cord-323428-jd91k19z 127 1 In in IN cord-323428-jd91k19z 127 2 terms term NNS cord-323428-jd91k19z 127 3 of of IN cord-323428-jd91k19z 127 4 IL-12 IL-12 NNP cord-323428-jd91k19z 127 5 production production NN cord-323428-jd91k19z 127 6 , , , cord-323428-jd91k19z 127 7 in in IN cord-323428-jd91k19z 127 8 vitro vitro FW cord-323428-jd91k19z 127 9 stimulation stimulation NN cord-323428-jd91k19z 127 10 with with IN cord-323428-jd91k19z 127 11 the the DT cord-323428-jd91k19z 127 12 MERS MERS NNP cord-323428-jd91k19z 127 13 - - HYPH cord-323428-jd91k19z 127 14 specific specific JJ cord-323428-jd91k19z 127 15 peptide peptide NN cord-323428-jd91k19z 127 16 " " `` cord-323428-jd91k19z 127 17 CYSSLILDY CYSSLILDY NNP cord-323428-jd91k19z 127 18 " " '' cord-323428-jd91k19z 127 19 resulted result VBD cord-323428-jd91k19z 127 20 in in IN cord-323428-jd91k19z 127 21 a a DT cord-323428-jd91k19z 127 22 significant significant JJ cord-323428-jd91k19z 127 23 ( ( -LRB- cord-323428-jd91k19z 127 24 p<0.05 p<0.05 CD cord-323428-jd91k19z 127 25 ) ) -RRB- cord-323428-jd91k19z 127 26 upregulation upregulation NN cord-323428-jd91k19z 127 27 in in IN cord-323428-jd91k19z 127 28 production production NN cord-323428-jd91k19z 127 29 when when WRB cord-323428-jd91k19z 127 30 compared compare VBN cord-323428-jd91k19z 127 31 with with IN cord-323428-jd91k19z 127 32 the the DT cord-323428-jd91k19z 127 33 antigen antigen NN cord-323428-jd91k19z 127 34 - - HYPH cord-323428-jd91k19z 127 35 stimulated stimulate VBN cord-323428-jd91k19z 127 36 and and CC cord-323428-jd91k19z 127 37 non non JJ cord-323428-jd91k19z 127 38 - - JJ cord-323428-jd91k19z 127 39 stimulated stimulated JJ cord-323428-jd91k19z 127 40 cell cell NN cord-323428-jd91k19z 127 41 culture culture NN cord-323428-jd91k19z 127 42 in in IN cord-323428-jd91k19z 127 43 the the DT cord-323428-jd91k19z 127 44 vaccinated vaccinated JJ cord-323428-jd91k19z 127 45 group group NN cord-323428-jd91k19z 127 46 only only RB cord-323428-jd91k19z 127 47 ( ( -LRB- cord-323428-jd91k19z 127 48 Figure-4 Figure-4 NNP cord-323428-jd91k19z 127 49 ) ) -RRB- cord-323428-jd91k19z 127 50 . . . cord-323428-jd91k19z 128 1 Although although IN cord-323428-jd91k19z 128 2 there there EX cord-323428-jd91k19z 128 3 was be VBD cord-323428-jd91k19z 128 4 no no DT cord-323428-jd91k19z 128 5 significant significant JJ cord-323428-jd91k19z 128 6 difference difference NN cord-323428-jd91k19z 128 7 in in IN cord-323428-jd91k19z 128 8 IL-4 IL-4 NNP cord-323428-jd91k19z 128 9 production production NN cord-323428-jd91k19z 128 10 between between IN cord-323428-jd91k19z 128 11 the the DT cord-323428-jd91k19z 128 12 control control NN cord-323428-jd91k19z 128 13 groups group NNS cord-323428-jd91k19z 128 14 and and CC cord-323428-jd91k19z 128 15 the the DT cord-323428-jd91k19z 128 16 vaccinated vaccinated JJ cord-323428-jd91k19z 128 17 group group NN cord-323428-jd91k19z 128 18 in in IN cord-323428-jd91k19z 128 19 non non JJ cord-323428-jd91k19z 128 20 - - JJ cord-323428-jd91k19z 128 21 stimulated stimulated JJ cord-323428-jd91k19z 128 22 cell cell NN cord-323428-jd91k19z 128 23 culture culture NN cord-323428-jd91k19z 128 24 , , , cord-323428-jd91k19z 128 25 antigen antigen NN cord-323428-jd91k19z 128 26 - - HYPH cord-323428-jd91k19z 128 27 specific specific JJ cord-323428-jd91k19z 128 28 stimulation stimulation NN cord-323428-jd91k19z 128 29 clearly clearly RB cord-323428-jd91k19z 128 30 revealed reveal VBD cord-323428-jd91k19z 128 31 a a DT cord-323428-jd91k19z 128 32 higher high JJR cord-323428-jd91k19z 128 33 production production NN cord-323428-jd91k19z 128 34 of of IN cord-323428-jd91k19z 128 35 this this DT cord-323428-jd91k19z 128 36 cytokine cytokine NN cord-323428-jd91k19z 128 37 in in IN cord-323428-jd91k19z 128 38 the the DT cord-323428-jd91k19z 128 39 control control NN cord-323428-jd91k19z 128 40 groups group NNS cord-323428-jd91k19z 128 41 above above IN cord-323428-jd91k19z 128 42 the the DT cord-323428-jd91k19z 128 43 vaccinated vaccinated JJ cord-323428-jd91k19z 128 44 group group NN cord-323428-jd91k19z 128 45 level level NN cord-323428-jd91k19z 128 46 of of IN cord-323428-jd91k19z 128 47 production production NN cord-323428-jd91k19z 128 48 ( ( -LRB- cord-323428-jd91k19z 128 49 p<0.05 p<0.05 CD cord-323428-jd91k19z 128 50 ) ) -RRB- cord-323428-jd91k19z 128 51 . . . cord-323428-jd91k19z 129 1 In in IN cord-323428-jd91k19z 129 2 addition addition NN cord-323428-jd91k19z 129 3 , , , cord-323428-jd91k19z 129 4 in in IN cord-323428-jd91k19z 129 5 vitro vitro FW cord-323428-jd91k19z 129 6 stimulation stimulation NN cord-323428-jd91k19z 129 7 with with IN cord-323428-jd91k19z 129 8 the the DT cord-323428-jd91k19z 129 9 MERS MERS NNP cord-323428-jd91k19z 129 10 specific specific JJ cord-323428-jd91k19z 129 11 peptide peptide NN cord-323428-jd91k19z 129 12 " " `` cord-323428-jd91k19z 129 13 CYSSLILDY CYSSLILDY NNP cord-323428-jd91k19z 129 14 " " '' cord-323428-jd91k19z 129 15 resulted result VBD cord-323428-jd91k19z 129 16 in in IN cord-323428-jd91k19z 129 17 a a DT cord-323428-jd91k19z 129 18 significant significant JJ cord-323428-jd91k19z 129 19 upregulation upregulation NN cord-323428-jd91k19z 129 20 in in IN cord-323428-jd91k19z 129 21 this this DT cord-323428-jd91k19z 129 22 production production NN cord-323428-jd91k19z 129 23 of of IN cord-323428-jd91k19z 129 24 this this DT cord-323428-jd91k19z 129 25 cytokine cytokine NN cord-323428-jd91k19z 129 26 in in IN cord-323428-jd91k19z 129 27 the the DT cord-323428-jd91k19z 129 28 control control NN cord-323428-jd91k19z 129 29 groups group NNS cord-323428-jd91k19z 129 30 while while IN cord-323428-jd91k19z 129 31 the the DT cord-323428-jd91k19z 129 32 same same JJ cord-323428-jd91k19z 129 33 antigen antigen NN cord-323428-jd91k19z 129 34 stimulation stimulation NN cord-323428-jd91k19z 129 35 resulted result VBD cord-323428-jd91k19z 129 36 in in IN cord-323428-jd91k19z 129 37 a a DT cord-323428-jd91k19z 129 38 significant significant JJ cord-323428-jd91k19z 129 39 decrease decrease NN cord-323428-jd91k19z 129 40 in in IN cord-323428-jd91k19z 129 41 IL-4 IL-4 NNP cord-323428-jd91k19z 129 42 production production NN cord-323428-jd91k19z 129 43 in in IN cord-323428-jd91k19z 129 44 the the DT cord-323428-jd91k19z 129 45 vaccinated vaccinated JJ cord-323428-jd91k19z 129 46 group group NN cord-323428-jd91k19z 129 47 ( ( -LRB- cord-323428-jd91k19z 129 48 p<0.05 p<0.05 CD cord-323428-jd91k19z 129 49 ) ) -RRB- cord-323428-jd91k19z 129 50 ( ( -LRB- cord-323428-jd91k19z 129 51 Figure-4 Figure-4 NNP cord-323428-jd91k19z 129 52 ) ) -RRB- cord-323428-jd91k19z 129 53 . . . cord-323428-jd91k19z 130 1 At at IN cord-323428-jd91k19z 130 2 week week NN cord-323428-jd91k19z 130 3 4 4 CD cord-323428-jd91k19z 130 4 post post VBP cord-323428-jd91k19z 130 5 first first JJ cord-323428-jd91k19z 130 6 immunization immunization NN cord-323428-jd91k19z 130 7 , , , cord-323428-jd91k19z 130 8 the the DT cord-323428-jd91k19z 130 9 fold fold JJ cord-323428-jd91k19z 130 10 change change NN cord-323428-jd91k19z 130 11 in in IN cord-323428-jd91k19z 130 12 IFN IFN NNP cord-323428-jd91k19z 130 13 - - HYPH cord-323428-jd91k19z 130 14 γ γ NN cord-323428-jd91k19z 130 15 gene gene NN cord-323428-jd91k19z 130 16 expression expression NN cord-323428-jd91k19z 130 17 was be VBD cord-323428-jd91k19z 130 18 significantly significantly RB cord-323428-jd91k19z 130 19 higher high JJR cord-323428-jd91k19z 130 20 than than IN cord-323428-jd91k19z 130 21 that that DT cord-323428-jd91k19z 130 22 in in IN cord-323428-jd91k19z 130 23 the the DT cord-323428-jd91k19z 130 24 control control NN cord-323428-jd91k19z 130 25 groups group NNS cord-323428-jd91k19z 130 26 ( ( -LRB- cord-323428-jd91k19z 130 27 Ad Ad NNP cord-323428-jd91k19z 130 28 - - HYPH cord-323428-jd91k19z 130 29 CMV CMV NNP cord-323428-jd91k19z 130 30 and and CC cord-323428-jd91k19z 130 31 PBS PBS NNP cord-323428-jd91k19z 130 32 ) ) -RRB- cord-323428-jd91k19z 131 1 ( ( -LRB- cord-323428-jd91k19z 131 2 p<0.05 p<0.05 CD cord-323428-jd91k19z 131 3 ) ) -RRB- cord-323428-jd91k19z 131 4 . . . cord-323428-jd91k19z 132 1 However however RB cord-323428-jd91k19z 132 2 , , , cord-323428-jd91k19z 132 3 there there EX cord-323428-jd91k19z 132 4 was be VBD cord-323428-jd91k19z 132 5 a a DT cord-323428-jd91k19z 132 6 prominent prominent JJ cord-323428-jd91k19z 132 7 increase increase NN cord-323428-jd91k19z 132 8 in in IN cord-323428-jd91k19z 132 9 IFN IFN NNP cord-323428-jd91k19z 132 10 - - HYPH cord-323428-jd91k19z 132 11 γ γ NN cord-323428-jd91k19z 132 12 gene gene NN cord-323428-jd91k19z 132 13 expression expression NN cord-323428-jd91k19z 132 14 at at IN cord-323428-jd91k19z 132 15 week week NN cord-323428-jd91k19z 132 16 6 6 CD cord-323428-jd91k19z 132 17 postprime postprime NN cord-323428-jd91k19z 132 18 in in IN cord-323428-jd91k19z 132 19 both both CC cord-323428-jd91k19z 132 20 the the DT cord-323428-jd91k19z 132 21 vaccinated vaccinated JJ cord-323428-jd91k19z 132 22 and and CC cord-323428-jd91k19z 132 23 Ad Ad NNP cord-323428-jd91k19z 132 24 - - HYPH cord-323428-jd91k19z 132 25 CMV CMV NNP cord-323428-jd91k19z 132 26 groups group NNS cord-323428-jd91k19z 132 27 compared compare VBN cord-323428-jd91k19z 132 28 to to IN cord-323428-jd91k19z 132 29 that that DT cord-323428-jd91k19z 132 30 in in IN cord-323428-jd91k19z 132 31 week week NN cord-323428-jd91k19z 132 32 4 4 CD cord-323428-jd91k19z 132 33 ( ( -LRB- cord-323428-jd91k19z 132 34 p<0.05 p<0.05 CD cord-323428-jd91k19z 132 35 ) ) -RRB- cord-323428-jd91k19z 132 36 . . . cord-323428-jd91k19z 133 1 The the DT cord-323428-jd91k19z 133 2 expression expression NN cord-323428-jd91k19z 133 3 of of IN cord-323428-jd91k19z 133 4 IFN IFN NNP cord-323428-jd91k19z 133 5 - - HYPH cord-323428-jd91k19z 133 6 γ γ NNP cord-323428-jd91k19z 133 7 was be VBD cord-323428-jd91k19z 133 8 slightly slightly RB cord-323428-jd91k19z 133 9 higher high JJR cord-323428-jd91k19z 133 10 but but CC cord-323428-jd91k19z 133 11 not not RB cord-323428-jd91k19z 133 12 statistically statistically RB cord-323428-jd91k19z 133 13 significant significant JJ cord-323428-jd91k19z 133 14 in in IN cord-323428-jd91k19z 133 15 the the DT cord-323428-jd91k19z 133 16 vaccinated vaccinated JJ cord-323428-jd91k19z 133 17 group group NN cord-323428-jd91k19z 133 18 compared compare VBN cord-323428-jd91k19z 133 19 to to IN cord-323428-jd91k19z 133 20 that that DT cord-323428-jd91k19z 133 21 in in IN cord-323428-jd91k19z 133 22 the the DT cord-323428-jd91k19z 133 23 Ad Ad NNP cord-323428-jd91k19z 133 24 - - HYPH cord-323428-jd91k19z 133 25 CMV CMV NNP cord-323428-jd91k19z 133 26 group group NN cord-323428-jd91k19z 133 27 at at IN cord-323428-jd91k19z 133 28 week week NN cord-323428-jd91k19z 133 29 6 6 CD cord-323428-jd91k19z 134 1 ( ( -LRB- cord-323428-jd91k19z 134 2 p>0.05 p>0.05 NNP cord-323428-jd91k19z 134 3 ) ) -RRB- cord-323428-jd91k19z 135 1 ( ( -LRB- cord-323428-jd91k19z 135 2 Figure-5 Figure-5 NNP cord-323428-jd91k19z 135 3 ) ) -RRB- cord-323428-jd91k19z 135 4 . . . cord-323428-jd91k19z 136 1 The the DT cord-323428-jd91k19z 136 2 only only JJ cord-323428-jd91k19z 136 3 significant significant JJ cord-323428-jd91k19z 136 4 change change NN cord-323428-jd91k19z 136 5 ( ( -LRB- cord-323428-jd91k19z 136 6 p<0.05 p<0.05 CD cord-323428-jd91k19z 136 7 ) ) -RRB- cord-323428-jd91k19z 136 8 in in IN cord-323428-jd91k19z 136 9 IL-4 IL-4 NNP cord-323428-jd91k19z 136 10 gene gene NN cord-323428-jd91k19z 136 11 expression expression NN cord-323428-jd91k19z 136 12 was be VBD cord-323428-jd91k19z 136 13 observed observe VBN cord-323428-jd91k19z 136 14 in in IN cord-323428-jd91k19z 136 15 the the DT cord-323428-jd91k19z 136 16 Ad Ad NNP cord-323428-jd91k19z 136 17 - - HYPH cord-323428-jd91k19z 136 18 CMV CMV NNP cord-323428-jd91k19z 136 19 group group NN cord-323428-jd91k19z 136 20 at at IN cord-323428-jd91k19z 136 21 week week NN cord-323428-jd91k19z 136 22 6 6 CD cord-323428-jd91k19z 136 23 ( ( -LRB- cord-323428-jd91k19z 136 24 Figure-5 Figure-5 NNP cord-323428-jd91k19z 136 25 ) ) -RRB- cord-323428-jd91k19z 136 26 while while IN cord-323428-jd91k19z 136 27 no no DT cord-323428-jd91k19z 136 28 significant significant JJ cord-323428-jd91k19z 136 29 changes change NNS cord-323428-jd91k19z 136 30 were be VBD cord-323428-jd91k19z 136 31 observed observe VBN cord-323428-jd91k19z 136 32 in in IN cord-323428-jd91k19z 136 33 the the DT cord-323428-jd91k19z 136 34 vaccinated vaccinated JJ cord-323428-jd91k19z 136 35 group group NN cord-323428-jd91k19z 136 36 at at IN cord-323428-jd91k19z 136 37 any any DT cord-323428-jd91k19z 136 38 time time NN cord-323428-jd91k19z 136 39 point point NN cord-323428-jd91k19z 136 40 . . . cord-323428-jd91k19z 137 1 In in IN cord-323428-jd91k19z 137 2 this this DT cord-323428-jd91k19z 137 3 study study NN cord-323428-jd91k19z 137 4 , , , cord-323428-jd91k19z 137 5 potentially potentially RB cord-323428-jd91k19z 137 6 protective protective JJ cord-323428-jd91k19z 137 7 humoral humoral JJ cord-323428-jd91k19z 137 8 and and CC cord-323428-jd91k19z 137 9 cellular cellular JJ cord-323428-jd91k19z 137 10 immune immune JJ cord-323428-jd91k19z 137 11 responses response NNS cord-323428-jd91k19z 137 12 were be VBD cord-323428-jd91k19z 137 13 elicited elicit VBN cord-323428-jd91k19z 137 14 in in IN cord-323428-jd91k19z 137 15 mice mouse NNS cord-323428-jd91k19z 137 16 by by IN cord-323428-jd91k19z 137 17 immunization immunization NN cord-323428-jd91k19z 137 18 using use VBG cord-323428-jd91k19z 137 19 a a DT cord-323428-jd91k19z 137 20 recombinant recombinant JJ cord-323428-jd91k19z 137 21 adenoviral adenoviral NN cord-323428-jd91k19z 137 22 - - HYPH cord-323428-jd91k19z 137 23 based base VBN cord-323428-jd91k19z 137 24 vaccine vaccine NN cord-323428-jd91k19z 137 25 encoding encode VBG cord-323428-jd91k19z 137 26 the the DT cord-323428-jd91k19z 137 27 S1 S1 NNP cord-323428-jd91k19z 137 28 subunit subunit NN cord-323428-jd91k19z 137 29 of of IN cord-323428-jd91k19z 137 30 the the DT cord-323428-jd91k19z 137 31 MERS MERS NNP cord-323428-jd91k19z 137 32 - - HYPH cord-323428-jd91k19z 137 33 CoV CoV NNP cord-323428-jd91k19z 137 34 spike spike NN cord-323428-jd91k19z 137 35 gene gene NN cord-323428-jd91k19z 137 36 . . . cord-323428-jd91k19z 138 1 Several several JJ cord-323428-jd91k19z 138 2 studies study NNS cord-323428-jd91k19z 138 3 have have VBP cord-323428-jd91k19z 138 4 been be VBN cord-323428-jd91k19z 138 5 published publish VBN cord-323428-jd91k19z 138 6 that that WDT cord-323428-jd91k19z 138 7 constructed construct VBD cord-323428-jd91k19z 138 8 similar similar JJ cord-323428-jd91k19z 138 9 vaccines vaccine NNS cord-323428-jd91k19z 138 10 against against IN cord-323428-jd91k19z 138 11 MERS MERS NNP cord-323428-jd91k19z 138 12 - - HYPH cord-323428-jd91k19z 138 13 CoV CoV NNP cord-323428-jd91k19z 138 14 and and CC cord-323428-jd91k19z 138 15 tested test VBD cord-323428-jd91k19z 138 16 their -PRON- PRP$ cord-323428-jd91k19z 138 17 ability ability NN cord-323428-jd91k19z 138 18 to to TO cord-323428-jd91k19z 138 19 induce induce VB cord-323428-jd91k19z 138 20 production production NN cord-323428-jd91k19z 138 21 of of IN cord-323428-jd91k19z 138 22 neutralizing neutralize VBG cord-323428-jd91k19z 138 23 antibodies antibody NNS cord-323428-jd91k19z 138 24 and and CC cord-323428-jd91k19z 138 25 other other JJ cord-323428-jd91k19z 138 26 immune immune JJ cord-323428-jd91k19z 138 27 system system NN cord-323428-jd91k19z 138 28 components component NNS cord-323428-jd91k19z 138 29 [ [ -LRB- cord-323428-jd91k19z 138 30 13 13 CD cord-323428-jd91k19z 138 31 , , , cord-323428-jd91k19z 138 32 14 14 CD cord-323428-jd91k19z 138 33 , , , cord-323428-jd91k19z 138 34 17 17 CD cord-323428-jd91k19z 138 35 , , , cord-323428-jd91k19z 138 36 [ [ -LRB- cord-323428-jd91k19z 138 37 19 19 CD cord-323428-jd91k19z 138 38 ] ] -RRB- cord-323428-jd91k19z 138 39 [ [ -LRB- cord-323428-jd91k19z 138 40 20 20 CD cord-323428-jd91k19z 138 41 ] ] -RRB- cord-323428-jd91k19z 138 42 [ [ -LRB- cord-323428-jd91k19z 138 43 21 21 CD cord-323428-jd91k19z 138 44 ] ] -RRB- cord-323428-jd91k19z 138 45 [ [ -LRB- cord-323428-jd91k19z 138 46 22 22 CD cord-323428-jd91k19z 138 47 ] ] -RRB- cord-323428-jd91k19z 138 48 [ [ -LRB- cord-323428-jd91k19z 138 49 23 23 CD cord-323428-jd91k19z 138 50 ] ] -RRB- cord-323428-jd91k19z 138 51 . . . cord-323428-jd91k19z 139 1 The the DT cord-323428-jd91k19z 139 2 results result NNS cord-323428-jd91k19z 139 3 of of IN cord-323428-jd91k19z 139 4 these these DT cord-323428-jd91k19z 139 5 studies study NNS cord-323428-jd91k19z 139 6 were be VBD cord-323428-jd91k19z 139 7 encouraging encouraging JJ cord-323428-jd91k19z 139 8 and and CC cord-323428-jd91k19z 139 9 showed show VBD cord-323428-jd91k19z 139 10 that that IN cord-323428-jd91k19z 139 11 12 12 CD cord-323428-jd91k19z 139 12 ] ] -RRB- cord-323428-jd91k19z 139 13 , , , cord-323428-jd91k19z 139 14 and and CC cord-323428-jd91k19z 139 15 IL-4 IL-4 NNP cord-323428-jd91k19z 139 16 ) ) -RRB- cord-323428-jd91k19z 140 1 in in IN cord-323428-jd91k19z 140 2 vitro vitro FW cord-323428-jd91k19z 140 3 . . . cord-323428-jd91k19z 141 1 Regardless regardless RB cord-323428-jd91k19z 141 2 of of IN cord-323428-jd91k19z 141 3 in in IN cord-323428-jd91k19z 141 4 vitro vitro FW cord-323428-jd91k19z 141 5 stimulation stimulation NN cord-323428-jd91k19z 141 6 , , , cord-323428-jd91k19z 141 7 production production NN cord-323428-jd91k19z 141 8 of of IN cord-323428-jd91k19z 141 9 IFN IFN NNP cord-323428-jd91k19z 141 10 - - HYPH cord-323428-jd91k19z 141 11 γ γ NNP cord-323428-jd91k19z 141 12 was be VBD cord-323428-jd91k19z 141 13 significantly significantly RB cord-323428-jd91k19z 141 14 ( ( -LRB- cord-323428-jd91k19z 141 15 p<0.05 p<0.05 CD cord-323428-jd91k19z 141 16 ) ) -RRB- cord-323428-jd91k19z 141 17 higher high JJR cord-323428-jd91k19z 141 18 in in IN cord-323428-jd91k19z 141 19 the the DT cord-323428-jd91k19z 141 20 vaccinated vaccinated JJ cord-323428-jd91k19z 141 21 group group NN cord-323428-jd91k19z 141 22 with with IN cord-323428-jd91k19z 141 23 Ad Ad NNP cord-323428-jd91k19z 141 24 - - HYPH cord-323428-jd91k19z 141 25 Middle Middle NNP cord-323428-jd91k19z 141 26 East East NNP cord-323428-jd91k19z 141 27 respiratory respiratory JJ cord-323428-jd91k19z 141 28 syndrome syndrome NN cord-323428-jd91k19z 141 29 ( ( -LRB- cord-323428-jd91k19z 141 30 MERS)-S1 MERS)-S1 NNS cord-323428-jd91k19z 141 31 compared compare VBN cord-323428-jd91k19z 141 32 with with IN cord-323428-jd91k19z 141 33 the the DT cord-323428-jd91k19z 141 34 control control NN cord-323428-jd91k19z 141 35 groups group NNS cord-323428-jd91k19z 141 36 ( ( -LRB- cord-323428-jd91k19z 141 37 Ad ad NN cord-323428-jd91k19z 141 38 - - HYPH cord-323428-jd91k19z 141 39 cytomegalovirus cytomegalovirus NN cord-323428-jd91k19z 141 40 and and CC cord-323428-jd91k19z 141 41 phosphate phosphate NN cord-323428-jd91k19z 141 42 - - HYPH cord-323428-jd91k19z 141 43 buffered buffer VBN cord-323428-jd91k19z 141 44 saline saline NN cord-323428-jd91k19z 141 45 ) ) -RRB- cord-323428-jd91k19z 141 46 at at RB cord-323428-jd91k19z 141 47 all all RB cord-323428-jd91k19z 141 48 tested test VBN cord-323428-jd91k19z 141 49 time time NN cord-323428-jd91k19z 141 50 points point NNS cord-323428-jd91k19z 141 51 . . . cord-323428-jd91k19z 142 1 In in IN cord-323428-jd91k19z 142 2 vitro vitro FW cord-323428-jd91k19z 142 3 , , , cord-323428-jd91k19z 142 4 antigen antigen NN cord-323428-jd91k19z 142 5 - - HYPH cord-323428-jd91k19z 142 6 specific specific JJ cord-323428-jd91k19z 142 7 stimulation stimulation NN cord-323428-jd91k19z 142 8 resulted result VBD cord-323428-jd91k19z 142 9 in in IN cord-323428-jd91k19z 142 10 a a DT cord-323428-jd91k19z 142 11 significant significant JJ cord-323428-jd91k19z 142 12 upregulation upregulation NN cord-323428-jd91k19z 142 13 of of IN cord-323428-jd91k19z 142 14 IL-12 IL-12 NNP cord-323428-jd91k19z 142 15 production production NN cord-323428-jd91k19z 142 16 when when WRB cord-323428-jd91k19z 142 17 compared compare VBN cord-323428-jd91k19z 142 18 with with IN cord-323428-jd91k19z 142 19 the the DT cord-323428-jd91k19z 142 20 antigen antigen NN cord-323428-jd91k19z 142 21 - - HYPH cord-323428-jd91k19z 142 22 stimulated stimulate VBN cord-323428-jd91k19z 142 23 and and CC cord-323428-jd91k19z 142 24 non non JJ cord-323428-jd91k19z 142 25 - - JJ cord-323428-jd91k19z 142 26 stimulated stimulated JJ cord-323428-jd91k19z 142 27 cell cell NN cord-323428-jd91k19z 142 28 culture culture NN cord-323428-jd91k19z 142 29 in in IN cord-323428-jd91k19z 142 30 the the DT cord-323428-jd91k19z 142 31 vaccinated vaccinated JJ cord-323428-jd91k19z 142 32 group group NN cord-323428-jd91k19z 142 33 only only RB cord-323428-jd91k19z 142 34 . . . cord-323428-jd91k19z 143 1 In in IN cord-323428-jd91k19z 143 2 vitro vitro FW cord-323428-jd91k19z 143 3 stimulation stimulation NN cord-323428-jd91k19z 143 4 using use VBG cord-323428-jd91k19z 143 5 the the DT cord-323428-jd91k19z 143 6 MERS MERS NNP cord-323428-jd91k19z 143 7 - - HYPH cord-323428-jd91k19z 143 8 specific specific JJ cord-323428-jd91k19z 143 9 peptide peptide NN cord-323428-jd91k19z 143 10 " " `` cord-323428-jd91k19z 143 11 CYSSLILDY CYSSLILDY NNP cord-323428-jd91k19z 143 12 " " '' cord-323428-jd91k19z 143 13 resulted result VBD cord-323428-jd91k19z 143 14 in in IN cord-323428-jd91k19z 143 15 a a DT cord-323428-jd91k19z 143 16 significant significant JJ cord-323428-jd91k19z 143 17 upregulation upregulation NN cord-323428-jd91k19z 143 18 of of IN cord-323428-jd91k19z 143 19 IL-4 IL-4 NNP cord-323428-jd91k19z 143 20 production production NN cord-323428-jd91k19z 143 21 in in IN cord-323428-jd91k19z 143 22 the the DT cord-323428-jd91k19z 143 23 control control NN cord-323428-jd91k19z 143 24 groups group NNS cord-323428-jd91k19z 143 25 while while IN cord-323428-jd91k19z 143 26 the the DT cord-323428-jd91k19z 143 27 same same JJ cord-323428-jd91k19z 143 28 antigen antigen NN cord-323428-jd91k19z 143 29 stimulation stimulation NN cord-323428-jd91k19z 143 30 resulted result VBD cord-323428-jd91k19z 143 31 in in IN cord-323428-jd91k19z 143 32 a a DT cord-323428-jd91k19z 143 33 significant significant JJ cord-323428-jd91k19z 143 34 decrease decrease NN cord-323428-jd91k19z 143 35 in in IN cord-323428-jd91k19z 143 36 IL-4 IL-4 NNP cord-323428-jd91k19z 143 37 production production NN cord-323428-jd91k19z 143 38 in in IN cord-323428-jd91k19z 143 39 the the DT cord-323428-jd91k19z 143 40 vaccinated vaccinated JJ cord-323428-jd91k19z 143 41 group group NN cord-323428-jd91k19z 143 42 . . . cord-323428-jd91k19z 144 1 NS NS NNP cord-323428-jd91k19z 144 2 : : : cord-323428-jd91k19z 145 1 Non non JJ cord-323428-jd91k19z 145 2 - - NN cord-323428-jd91k19z 145 3 antigen antigen NN cord-323428-jd91k19z 145 4 stimulated stimulate VBN cord-323428-jd91k19z 145 5 , , , cord-323428-jd91k19z 145 6 Ag Ag NNP cord-323428-jd91k19z 145 7 : : : cord-323428-jd91k19z 145 8 Antigen antigen NN cord-323428-jd91k19z 145 9 - - HYPH cord-323428-jd91k19z 145 10 stimulated stimulate VBN cord-323428-jd91k19z 145 11 . . . cord-323428-jd91k19z 146 1 Different different JJ cord-323428-jd91k19z 146 2 letters letter NNS cord-323428-jd91k19z 146 3 mean mean VBP cord-323428-jd91k19z 146 4 significantly significantly RB cord-323428-jd91k19z 146 5 different different JJ cord-323428-jd91k19z 146 6 at at IN cord-323428-jd91k19z 146 7 p<0.05 p<0.05 CD cord-323428-jd91k19z 146 8 . . . cord-323428-jd91k19z 147 1 Available available JJ cord-323428-jd91k19z 147 2 at at IN cord-323428-jd91k19z 147 3 www.veterinaryworld.org/Vol.12/October-2019/7.pdf www.veterinaryworld.org/Vol.12/October-2019/7.pdf NNP cord-323428-jd91k19z 148 1 such such JJ cord-323428-jd91k19z 148 2 vaccines vaccine NNS cord-323428-jd91k19z 148 3 may may MD cord-323428-jd91k19z 148 4 be be VB cord-323428-jd91k19z 148 5 promising promise VBG cord-323428-jd91k19z 148 6 to to TO cord-323428-jd91k19z 148 7 prevent prevent VB cord-323428-jd91k19z 148 8 the the DT cord-323428-jd91k19z 148 9 global global JJ cord-323428-jd91k19z 148 10 threat threat NN cord-323428-jd91k19z 148 11 of of IN cord-323428-jd91k19z 148 12 MERS MERS NNP cord-323428-jd91k19z 148 13 - - HYPH cord-323428-jd91k19z 148 14 CoV CoV NNP cord-323428-jd91k19z 148 15 infection infection NN cord-323428-jd91k19z 148 16 [ [ -LRB- cord-323428-jd91k19z 148 17 13 13 CD cord-323428-jd91k19z 148 18 , , , cord-323428-jd91k19z 148 19 14 14 CD cord-323428-jd91k19z 148 20 , , , cord-323428-jd91k19z 148 21 17 17 CD cord-323428-jd91k19z 148 22 , , , cord-323428-jd91k19z 148 23 [ [ -LRB- cord-323428-jd91k19z 148 24 19 19 CD cord-323428-jd91k19z 148 25 ] ] -RRB- cord-323428-jd91k19z 148 26 [ [ -LRB- cord-323428-jd91k19z 148 27 20 20 CD cord-323428-jd91k19z 148 28 ] ] -RRB- cord-323428-jd91k19z 148 29 [ [ -LRB- cord-323428-jd91k19z 148 30 21 21 CD cord-323428-jd91k19z 148 31 ] ] -RRB- cord-323428-jd91k19z 148 32 [ [ -LRB- cord-323428-jd91k19z 148 33 22 22 CD cord-323428-jd91k19z 148 34 ] ] -RRB- cord-323428-jd91k19z 148 35 [ [ -LRB- cord-323428-jd91k19z 148 36 23 23 CD cord-323428-jd91k19z 148 37 ] ] -RRB- cord-323428-jd91k19z 148 38 . . . cord-323428-jd91k19z 149 1 However however RB cord-323428-jd91k19z 149 2 , , , cord-323428-jd91k19z 149 3 the the DT cord-323428-jd91k19z 149 4 safety safety NN cord-323428-jd91k19z 149 5 and and CC cord-323428-jd91k19z 149 6 efficacy efficacy NN cord-323428-jd91k19z 149 7 of of IN cord-323428-jd91k19z 149 8 these these DT cord-323428-jd91k19z 149 9 vaccines vaccine NNS cord-323428-jd91k19z 149 10 still still RB cord-323428-jd91k19z 149 11 to to TO cord-323428-jd91k19z 149 12 be be VB cord-323428-jd91k19z 149 13 confirmed confirm VBN cord-323428-jd91k19z 149 14 . . . cord-323428-jd91k19z 150 1 It -PRON- PRP cord-323428-jd91k19z 150 2 was be VBD cord-323428-jd91k19z 150 3 confirmed confirm VBN cord-323428-jd91k19z 150 4 that that IN cord-323428-jd91k19z 150 5 the the DT cord-323428-jd91k19z 150 6 S S NNP cord-323428-jd91k19z 150 7 protein protein NN cord-323428-jd91k19z 150 8 is be VBZ cord-323428-jd91k19z 150 9 responsible responsible JJ cord-323428-jd91k19z 150 10 for for IN cord-323428-jd91k19z 150 11 the the DT cord-323428-jd91k19z 150 12 production production NN cord-323428-jd91k19z 150 13 of of IN cord-323428-jd91k19z 150 14 neutralizing neutralize VBG cord-323428-jd91k19z 150 15 antibodies antibody NNS cord-323428-jd91k19z 150 16 [ [ -LRB- cord-323428-jd91k19z 150 17 22 22 CD cord-323428-jd91k19z 150 18 , , , cord-323428-jd91k19z 150 19 31 31 CD cord-323428-jd91k19z 150 20 ] ] -RRB- cord-323428-jd91k19z 150 21 . . . cord-323428-jd91k19z 151 1 The the DT cord-323428-jd91k19z 151 2 S1 S1 NNP cord-323428-jd91k19z 151 3 subunit subunit NN cord-323428-jd91k19z 151 4 contains contain VBZ cord-323428-jd91k19z 151 5 the the DT cord-323428-jd91k19z 151 6 RBD RBD NNP cord-323428-jd91k19z 151 7 , , , cord-323428-jd91k19z 151 8 which which WDT cord-323428-jd91k19z 151 9 binds bind VBZ cord-323428-jd91k19z 151 10 to to IN cord-323428-jd91k19z 151 11 its -PRON- PRP$ cord-323428-jd91k19z 151 12 host host NN cord-323428-jd91k19z 151 13 receptor receptor NN cord-323428-jd91k19z 151 14 DPP4 DPP4 NNP cord-323428-jd91k19z 151 15 ( ( -LRB- cord-323428-jd91k19z 151 16 also also RB cord-323428-jd91k19z 151 17 known know VBN cord-323428-jd91k19z 151 18 as as IN cord-323428-jd91k19z 151 19 CD26 CD26 NNP cord-323428-jd91k19z 151 20 ) ) -RRB- cord-323428-jd91k19z 151 21 and and CC cord-323428-jd91k19z 151 22 induces induce VBZ cord-323428-jd91k19z 151 23 production production NN cord-323428-jd91k19z 151 24 of of IN cord-323428-jd91k19z 151 25 specific specific JJ cord-323428-jd91k19z 151 26 neutralizing neutralize VBG cord-323428-jd91k19z 151 27 antibodies antibody NNS cord-323428-jd91k19z 151 28 . . . cord-323428-jd91k19z 152 1 Several several JJ cord-323428-jd91k19z 152 2 studies study NNS cord-323428-jd91k19z 152 3 have have VBP cord-323428-jd91k19z 152 4 supported support VBN cord-323428-jd91k19z 152 5 the the DT cord-323428-jd91k19z 152 6 role role NN cord-323428-jd91k19z 152 7 of of IN cord-323428-jd91k19z 152 8 this this DT cord-323428-jd91k19z 152 9 domain domain NN cord-323428-jd91k19z 152 10 to to TO cord-323428-jd91k19z 152 11 elicit elicit VB cord-323428-jd91k19z 152 12 protective protective JJ cord-323428-jd91k19z 152 13 immunity immunity NN cord-323428-jd91k19z 152 14 against against IN cord-323428-jd91k19z 152 15 MERS MERS NNP cord-323428-jd91k19z 152 16 - - HYPH cord-323428-jd91k19z 152 17 CoV CoV NNP cord-323428-jd91k19z 152 18 challenge challenge NN cord-323428-jd91k19z 152 19 [ [ -LRB- cord-323428-jd91k19z 152 20 25 25 CD cord-323428-jd91k19z 152 21 , , , cord-323428-jd91k19z 152 22 36 36 CD cord-323428-jd91k19z 152 23 , , , cord-323428-jd91k19z 152 24 37 37 CD cord-323428-jd91k19z 152 25 ] ] -RRB- cord-323428-jd91k19z 152 26 . . . cord-323428-jd91k19z 153 1 In in IN cord-323428-jd91k19z 153 2 this this DT cord-323428-jd91k19z 153 3 study study NN cord-323428-jd91k19z 153 4 , , , cord-323428-jd91k19z 153 5 we -PRON- PRP cord-323428-jd91k19z 153 6 were be VBD cord-323428-jd91k19z 153 7 able able JJ cord-323428-jd91k19z 153 8 to to TO cord-323428-jd91k19z 153 9 demonstrate demonstrate VB cord-323428-jd91k19z 153 10 the the DT cord-323428-jd91k19z 153 11 distribution distribution NN cord-323428-jd91k19z 153 12 and and CC cord-323428-jd91k19z 153 13 expression expression NN cord-323428-jd91k19z 153 14 of of IN cord-323428-jd91k19z 153 15 the the DT cord-323428-jd91k19z 153 16 S1 S1 NNP cord-323428-jd91k19z 153 17 protein protein NN cord-323428-jd91k19z 153 18 in in IN cord-323428-jd91k19z 153 19 the the DT cord-323428-jd91k19z 153 20 vaccinated vaccinated JJ cord-323428-jd91k19z 153 21 group group NN cord-323428-jd91k19z 153 22 but but CC cord-323428-jd91k19z 153 23 not not RB cord-323428-jd91k19z 153 24 in in IN cord-323428-jd91k19z 153 25 the the DT cord-323428-jd91k19z 153 26 control control NN cord-323428-jd91k19z 153 27 groups group NNS cord-323428-jd91k19z 153 28 ( ( -LRB- cord-323428-jd91k19z 153 29 Ad Ad NNP cord-323428-jd91k19z 153 30 - - HYPH cord-323428-jd91k19z 153 31 CMV CMV NNP cord-323428-jd91k19z 153 32 and and CC cord-323428-jd91k19z 153 33 PBS PBS NNP cord-323428-jd91k19z 153 34 ) ) -RRB- cord-323428-jd91k19z 153 35 by by IN cord-323428-jd91k19z 153 36 detection detection NN cord-323428-jd91k19z 153 37 of of IN cord-323428-jd91k19z 153 38 the the DT cord-323428-jd91k19z 153 39 truncated truncated JJ cord-323428-jd91k19z 153 40 protein protein NN cord-323428-jd91k19z 153 41 - - HYPH cord-323428-jd91k19z 153 42 encoding encode VBG cord-323428-jd91k19z 153 43 segment segment NN cord-323428-jd91k19z 153 44 in in IN cord-323428-jd91k19z 153 45 kidney kidney NN cord-323428-jd91k19z 153 46 and and CC cord-323428-jd91k19z 153 47 lung lung NN cord-323428-jd91k19z 153 48 tissues tissue NNS cord-323428-jd91k19z 153 49 following follow VBG cord-323428-jd91k19z 153 50 vaccination vaccination NN cord-323428-jd91k19z 153 51 using use VBG cord-323428-jd91k19z 153 52 conventional conventional JJ cord-323428-jd91k19z 153 53 PCR PCR NNP cord-323428-jd91k19z 153 54 and and CC cord-323428-jd91k19z 153 55 IHC IHC NNP cord-323428-jd91k19z 153 56 . . . cord-323428-jd91k19z 154 1 These these DT cord-323428-jd91k19z 154 2 findings finding NNS cord-323428-jd91k19z 154 3 support support VBP cord-323428-jd91k19z 154 4 the the DT cord-323428-jd91k19z 154 5 ability ability NN cord-323428-jd91k19z 154 6 of of IN cord-323428-jd91k19z 154 7 this this DT cord-323428-jd91k19z 154 8 vaccine vaccine NN cord-323428-jd91k19z 154 9 candidate candidate NN cord-323428-jd91k19z 154 10 to to TO cord-323428-jd91k19z 154 11 distribute distribute VB cord-323428-jd91k19z 154 12 effectively effectively RB cord-323428-jd91k19z 154 13 within within IN cord-323428-jd91k19z 154 14 host host NN cord-323428-jd91k19z 154 15 tissues tissue NNS cord-323428-jd91k19z 154 16 and and CC cord-323428-jd91k19z 154 17 express express VB cord-323428-jd91k19z 154 18 the the DT cord-323428-jd91k19z 154 19 target target NN cord-323428-jd91k19z 154 20 protein protein NN cord-323428-jd91k19z 154 21 . . . cord-323428-jd91k19z 155 1 It -PRON- PRP cord-323428-jd91k19z 155 2 has have VBZ cord-323428-jd91k19z 155 3 been be VBN cord-323428-jd91k19z 155 4 established establish VBN cord-323428-jd91k19z 155 5 that that IN cord-323428-jd91k19z 155 6 DPP4 DPP4 NNP cord-323428-jd91k19z 155 7 is be VBZ cord-323428-jd91k19z 155 8 expressed express VBN cord-323428-jd91k19z 155 9 on on IN cord-323428-jd91k19z 155 10 multiple multiple JJ cord-323428-jd91k19z 155 11 cell cell NN cord-323428-jd91k19z 155 12 types type NNS cord-323428-jd91k19z 155 13 in in IN cord-323428-jd91k19z 155 14 the the DT cord-323428-jd91k19z 155 15 human human JJ cord-323428-jd91k19z 155 16 body body NN cord-323428-jd91k19z 155 17 including include VBG cord-323428-jd91k19z 155 18 cells cell NNS cord-323428-jd91k19z 155 19 in in IN cord-323428-jd91k19z 155 20 the the DT cord-323428-jd91k19z 155 21 kidneys kidney NNS cord-323428-jd91k19z 155 22 and and CC cord-323428-jd91k19z 155 23 lungs lung NNS cord-323428-jd91k19z 155 24 [ [ -LRB- cord-323428-jd91k19z 155 25 24 24 CD cord-323428-jd91k19z 155 26 ] ] -RRB- cord-323428-jd91k19z 155 27 . . . cord-323428-jd91k19z 156 1 However however RB cord-323428-jd91k19z 156 2 , , , cord-323428-jd91k19z 156 3 the the DT cord-323428-jd91k19z 156 4 N n CD cord-323428-jd91k19z 156 5 glycoprotein glycoprotein NN cord-323428-jd91k19z 156 6 , , , cord-323428-jd91k19z 156 7 but but CC cord-323428-jd91k19z 156 8 not not RB cord-323428-jd91k19z 156 9 the the DT cord-323428-jd91k19z 156 10 S S NNP cord-323428-jd91k19z 156 11 glycoprotein glycoprotein NN cord-323428-jd91k19z 156 12 , , , cord-323428-jd91k19z 156 13 of of IN cord-323428-jd91k19z 156 14 MERS MERS NNPS cord-323428-jd91k19z 156 15 was be VBD cord-323428-jd91k19z 156 16 the the DT cord-323428-jd91k19z 156 17 target target NN cord-323428-jd91k19z 156 18 of of IN cord-323428-jd91k19z 156 19 investigation investigation NN cord-323428-jd91k19z 156 20 of of IN cord-323428-jd91k19z 156 21 MERS MERS NNP cord-323428-jd91k19z 156 22 - - HYPH cord-323428-jd91k19z 156 23 CoV cov NN cord-323428-jd91k19z 156 24 localization localization NN cord-323428-jd91k19z 156 25 using use VBG cord-323428-jd91k19z 156 26 IHC IHC NNP cord-323428-jd91k19z 156 27 [ [ -LRB- cord-323428-jd91k19z 156 28 38 38 CD cord-323428-jd91k19z 156 29 ] ] -RRB- cord-323428-jd91k19z 156 30 . . . cord-323428-jd91k19z 157 1 The the DT cord-323428-jd91k19z 157 2 N N NNP cord-323428-jd91k19z 157 3 glycoprotein glycoprotein NN cord-323428-jd91k19z 157 4 was be VBD cord-323428-jd91k19z 157 5 clearly clearly RB cord-323428-jd91k19z 157 6 present present JJ cord-323428-jd91k19z 157 7 in in IN cord-323428-jd91k19z 157 8 lung lung NN cord-323428-jd91k19z 157 9 tissues tissue NNS cord-323428-jd91k19z 157 10 following follow VBG cord-323428-jd91k19z 157 11 MERS MERS NNP cord-323428-jd91k19z 157 12 exposure exposure NN cord-323428-jd91k19z 157 13 . . . cord-323428-jd91k19z 158 1 In in IN cord-323428-jd91k19z 158 2 fact fact NN cord-323428-jd91k19z 158 3 , , , cord-323428-jd91k19z 158 4 the the DT cord-323428-jd91k19z 158 5 current current JJ cord-323428-jd91k19z 158 6 study study NN cord-323428-jd91k19z 158 7 can can MD cord-323428-jd91k19z 158 8 be be VB cord-323428-jd91k19z 158 9 considered consider VBN cord-323428-jd91k19z 158 10 pioneering pioneer VBG cord-323428-jd91k19z 158 11 given give VBN cord-323428-jd91k19z 158 12 the the DT cord-323428-jd91k19z 158 13 use use NN cord-323428-jd91k19z 158 14 of of IN cord-323428-jd91k19z 158 15 the the DT cord-323428-jd91k19z 158 16 S1 s1 NN cord-323428-jd91k19z 158 17 glycoprotein glycoprotein NN cord-323428-jd91k19z 158 18 to to TO cord-323428-jd91k19z 158 19 express express VB cord-323428-jd91k19z 158 20 viral viral JJ cord-323428-jd91k19z 158 21 distribution distribution NN cord-323428-jd91k19z 158 22 in in IN cord-323428-jd91k19z 158 23 tissues tissue NNS cord-323428-jd91k19z 158 24 . . . cord-323428-jd91k19z 159 1 Therefore therefore RB cord-323428-jd91k19z 159 2 , , , cord-323428-jd91k19z 159 3 the the DT cord-323428-jd91k19z 159 4 expression expression NN cord-323428-jd91k19z 159 5 of of IN cord-323428-jd91k19z 159 6 S1 s1 NN cord-323428-jd91k19z 159 7 protein protein NN cord-323428-jd91k19z 159 8 in in IN cord-323428-jd91k19z 159 9 kidney kidney NN cord-323428-jd91k19z 159 10 tissues tissue NNS cord-323428-jd91k19z 159 11 and and CC cord-323428-jd91k19z 159 12 not not RB cord-323428-jd91k19z 159 13 in in IN cord-323428-jd91k19z 159 14 lung lung NN cord-323428-jd91k19z 159 15 might may MD cord-323428-jd91k19z 159 16 be be VB cord-323428-jd91k19z 159 17 used use VBN cord-323428-jd91k19z 159 18 to to TO cord-323428-jd91k19z 159 19 explain explain VB cord-323428-jd91k19z 159 20 previous previous JJ cord-323428-jd91k19z 159 21 in in IN cord-323428-jd91k19z 159 22 vitro vitro FW cord-323428-jd91k19z 159 23 experiments experiment NNS cord-323428-jd91k19z 159 24 , , , cord-323428-jd91k19z 159 25 which which WDT cord-323428-jd91k19z 159 26 found find VBD cord-323428-jd91k19z 159 27 a a DT cord-323428-jd91k19z 159 28 cytopathic cytopathic JJ cord-323428-jd91k19z 159 29 effect effect NN cord-323428-jd91k19z 159 30 of of IN cord-323428-jd91k19z 159 31 the the DT cord-323428-jd91k19z 159 32 MERS MERS NNP cord-323428-jd91k19z 159 33 - - HYPH cord-323428-jd91k19z 159 34 CoV cov NN cord-323428-jd91k19z 159 35 infection infection NN cord-323428-jd91k19z 159 36 inside inside IN cord-323428-jd91k19z 159 37 primary primary JJ cord-323428-jd91k19z 159 38 human human JJ cord-323428-jd91k19z 159 39 kidney kidney NN cord-323428-jd91k19z 159 40 cells cell NNS cord-323428-jd91k19z 159 41 but but CC cord-323428-jd91k19z 159 42 not not RB cord-323428-jd91k19z 159 43 in in IN cord-323428-jd91k19z 159 44 primary primary JJ cord-323428-jd91k19z 159 45 human human JJ cord-323428-jd91k19z 159 46 bronchial bronchial JJ cord-323428-jd91k19z 159 47 epithelial epithelial JJ cord-323428-jd91k19z 159 48 cells cell NNS cord-323428-jd91k19z 159 49 [ [ -LRB- cord-323428-jd91k19z 159 50 39 39 CD cord-323428-jd91k19z 159 51 ] ] -RRB- cord-323428-jd91k19z 159 52 . . . cord-323428-jd91k19z 160 1 In in IN cord-323428-jd91k19z 160 2 a a DT cord-323428-jd91k19z 160 3 recent recent JJ cord-323428-jd91k19z 160 4 study study NN cord-323428-jd91k19z 160 5 , , , cord-323428-jd91k19z 160 6 the the DT cord-323428-jd91k19z 160 7 adenoviral adenoviral JJ cord-323428-jd91k19z 160 8 vector vector NN cord-323428-jd91k19z 160 9 with with IN cord-323428-jd91k19z 160 10 enhanced enhanced JJ cord-323428-jd91k19z 160 11 green green JJ cord-323428-jd91k19z 160 12 fluorescent fluorescent NN cord-323428-jd91k19z 160 13 protein protein NN cord-323428-jd91k19z 160 14 but but CC cord-323428-jd91k19z 160 15 not not RB cord-323428-jd91k19z 160 16 the the DT cord-323428-jd91k19z 160 17 adenoviral adenoviral JJ cord-323428-jd91k19z 160 18 vector vector NN cord-323428-jd91k19z 160 19 with with IN cord-323428-jd91k19z 160 20 MERS MERS NNPS cord-323428-jd91k19z 160 21 in in IN cord-323428-jd91k19z 160 22 a a DT cord-323428-jd91k19z 160 23 hDPP4 hdpp4 NN cord-323428-jd91k19z 160 24 mice mouse NNS cord-323428-jd91k19z 160 25 experiment experiment NN cord-323428-jd91k19z 160 26 after after IN cord-323428-jd91k19z 160 27 intramuscular intramuscular JJ cord-323428-jd91k19z 160 28 or or CC cord-323428-jd91k19z 160 29 intranasal intranasal NN cord-323428-jd91k19z 160 30 injection injection NN cord-323428-jd91k19z 160 31 [ [ -LRB- cord-323428-jd91k19z 160 32 21 21 CD cord-323428-jd91k19z 160 33 ] ] -RRB- cord-323428-jd91k19z 160 34 . . . cord-323428-jd91k19z 161 1 This this DT cord-323428-jd91k19z 161 2 supporting support VBG cord-323428-jd91k19z 161 3 our -PRON- PRP$ cord-323428-jd91k19z 161 4 finding finding NN cord-323428-jd91k19z 161 5 that that IN cord-323428-jd91k19z 161 6 this this DT cord-323428-jd91k19z 161 7 construct construct NN cord-323428-jd91k19z 161 8 was be VBD cord-323428-jd91k19z 161 9 able able JJ cord-323428-jd91k19z 161 10 to to TO cord-323428-jd91k19z 161 11 reach reach VB cord-323428-jd91k19z 161 12 the the DT cord-323428-jd91k19z 161 13 lung lung NN cord-323428-jd91k19z 161 14 tissues tissue NNS cord-323428-jd91k19z 161 15 , , , cord-323428-jd91k19z 161 16 but but CC cord-323428-jd91k19z 161 17 the the DT cord-323428-jd91k19z 161 18 S1 s1 NN cord-323428-jd91k19z 161 19 protein protein NN cord-323428-jd91k19z 161 20 was be VBD cord-323428-jd91k19z 161 21 not not RB cord-323428-jd91k19z 161 22 expressed express VBN cord-323428-jd91k19z 161 23 in in IN cord-323428-jd91k19z 161 24 lung lung NN cord-323428-jd91k19z 161 25 tissues tissue NNS cord-323428-jd91k19z 161 26 . . . cord-323428-jd91k19z 162 1 However however RB cord-323428-jd91k19z 162 2 , , , cord-323428-jd91k19z 162 3 S1 s1 NN cord-323428-jd91k19z 162 4 gene gene NN cord-323428-jd91k19z 162 5 detection detection NN cord-323428-jd91k19z 162 6 in in IN cord-323428-jd91k19z 162 7 lung lung NN cord-323428-jd91k19z 162 8 tissues tissue NNS cord-323428-jd91k19z 162 9 by by IN cord-323428-jd91k19z 162 10 conventional conventional JJ cord-323428-jd91k19z 162 11 PCR PCR NNP cord-323428-jd91k19z 162 12 and and CC cord-323428-jd91k19z 162 13 not not RB cord-323428-jd91k19z 162 14 by by IN cord-323428-jd91k19z 162 15 IHC IHC NNP cord-323428-jd91k19z 162 16 indicates indicate VBZ cord-323428-jd91k19z 162 17 further further JJ cord-323428-jd91k19z 162 18 investigation investigation NN cord-323428-jd91k19z 162 19 is be VBZ cord-323428-jd91k19z 162 20 necessary necessary JJ cord-323428-jd91k19z 162 21 regarding regard VBG cord-323428-jd91k19z 162 22 the the DT cord-323428-jd91k19z 162 23 level level NN cord-323428-jd91k19z 162 24 and and CC cord-323428-jd91k19z 162 25 intensity intensity NN cord-323428-jd91k19z 162 26 of of IN cord-323428-jd91k19z 162 27 S1 s1 NN cord-323428-jd91k19z 162 28 expression expression NN cord-323428-jd91k19z 162 29 in in IN cord-323428-jd91k19z 162 30 this this DT cord-323428-jd91k19z 162 31 tissue tissue NN cord-323428-jd91k19z 162 32 . . . cord-323428-jd91k19z 163 1 Additional additional JJ cord-323428-jd91k19z 163 2 studies study NNS cord-323428-jd91k19z 163 3 are be VBP cord-323428-jd91k19z 163 4 also also RB cord-323428-jd91k19z 163 5 needed need VBN cord-323428-jd91k19z 163 6 to to TO cord-323428-jd91k19z 163 7 explore explore VB cord-323428-jd91k19z 163 8 S1 S1 NNP cord-323428-jd91k19z 163 9 protein protein NN cord-323428-jd91k19z 163 10 expression expression NN cord-323428-jd91k19z 163 11 in in IN cord-323428-jd91k19z 163 12 other other JJ cord-323428-jd91k19z 163 13 tissues tissue NNS cord-323428-jd91k19z 163 14 such such JJ cord-323428-jd91k19z 163 15 as as IN cord-323428-jd91k19z 163 16 the the DT cord-323428-jd91k19z 163 17 liver liver NN cord-323428-jd91k19z 163 18 and and CC cord-323428-jd91k19z 163 19 intestine intestine NN cord-323428-jd91k19z 163 20 given give VBN cord-323428-jd91k19z 163 21 the the DT cord-323428-jd91k19z 163 22 expression expression NN cord-323428-jd91k19z 163 23 of of IN cord-323428-jd91k19z 163 24 DPP4 DPP4 NNP cord-323428-jd91k19z 163 25 in in IN cord-323428-jd91k19z 163 26 these these DT cord-323428-jd91k19z 163 27 tissues tissue NNS cord-323428-jd91k19z 163 28 [ [ -LRB- cord-323428-jd91k19z 163 29 40 40 CD cord-323428-jd91k19z 163 30 ] ] -RRB- cord-323428-jd91k19z 163 31 . . . cord-323428-jd91k19z 164 1 In in IN cord-323428-jd91k19z 164 2 this this DT cord-323428-jd91k19z 164 3 study study NN cord-323428-jd91k19z 164 4 , , , cord-323428-jd91k19z 164 5 there there EX cord-323428-jd91k19z 164 6 was be VBD cord-323428-jd91k19z 164 7 a a DT cord-323428-jd91k19z 164 8 significant significant JJ cord-323428-jd91k19z 164 9 serum serum NN cord-323428-jd91k19z 164 10 level level NN cord-323428-jd91k19z 164 11 of of IN cord-323428-jd91k19z 164 12 the the DT cord-323428-jd91k19z 164 13 S1-specific S1-specific NNP cord-323428-jd91k19z 164 14 IgG igg NN cord-323428-jd91k19z 164 15 antibody antibody NN cord-323428-jd91k19z 164 16 in in IN cord-323428-jd91k19z 164 17 the the DT cord-323428-jd91k19z 164 18 vaccinated vaccinated JJ cord-323428-jd91k19z 164 19 group group NN cord-323428-jd91k19z 164 20 but but CC cord-323428-jd91k19z 164 21 not not RB cord-323428-jd91k19z 164 22 in in IN cord-323428-jd91k19z 164 23 the the DT cord-323428-jd91k19z 164 24 control control NN cord-323428-jd91k19z 164 25 groups group NNS cord-323428-jd91k19z 164 26 ( ( -LRB- cord-323428-jd91k19z 164 27 Ad Ad NNP cord-323428-jd91k19z 164 28 - - HYPH cord-323428-jd91k19z 164 29 CMV CMV NNP cord-323428-jd91k19z 164 30 and and CC cord-323428-jd91k19z 164 31 PBS PBS NNP cord-323428-jd91k19z 164 32 ) ) -RRB- cord-323428-jd91k19z 164 33 . . . cord-323428-jd91k19z 165 1 Similar similar JJ cord-323428-jd91k19z 165 2 results result NNS cord-323428-jd91k19z 165 3 have have VBP cord-323428-jd91k19z 165 4 been be VBN cord-323428-jd91k19z 165 5 shown show VBN cord-323428-jd91k19z 165 6 previously previously RB cord-323428-jd91k19z 165 7 : : : cord-323428-jd91k19z 165 8 The the DT cord-323428-jd91k19z 165 9 SARS SARS NNP cord-323428-jd91k19z 165 10 - - HYPH cord-323428-jd91k19z 165 11 CoV CoV NNP cord-323428-jd91k19z 165 12 vaccine vaccine NN cord-323428-jd91k19z 165 13 had have VBD cord-323428-jd91k19z 165 14 a a DT cord-323428-jd91k19z 165 15 strong strong JJ cord-323428-jd91k19z 165 16 ability ability NN cord-323428-jd91k19z 165 17 to to TO cord-323428-jd91k19z 165 18 elicit elicit VB cord-323428-jd91k19z 165 19 the the DT cord-323428-jd91k19z 165 20 S1-specific S1-specific NNP cord-323428-jd91k19z 165 21 IgG igg NN cord-323428-jd91k19z 165 22 antibody antibody NN cord-323428-jd91k19z 165 23 response response NN cord-323428-jd91k19z 165 24 [ [ -LRB- cord-323428-jd91k19z 165 25 26 26 CD cord-323428-jd91k19z 165 26 ] ] -RRB- cord-323428-jd91k19z 165 27 . . . cord-323428-jd91k19z 166 1 The the DT cord-323428-jd91k19z 166 2 results result NNS cord-323428-jd91k19z 166 3 presented present VBN cord-323428-jd91k19z 166 4 in in IN cord-323428-jd91k19z 166 5 this this DT cord-323428-jd91k19z 166 6 study study NN cord-323428-jd91k19z 166 7 also also RB cord-323428-jd91k19z 166 8 supported support VBD cord-323428-jd91k19z 166 9 the the DT cord-323428-jd91k19z 166 10 findings finding NNS cord-323428-jd91k19z 166 11 of of IN cord-323428-jd91k19z 166 12 recent recent JJ cord-323428-jd91k19z 166 13 publications publication NNS cord-323428-jd91k19z 166 14 applying apply VBG cord-323428-jd91k19z 166 15 a a DT cord-323428-jd91k19z 166 16 similar similar JJ cord-323428-jd91k19z 166 17 vector vector NN cord-323428-jd91k19z 166 18 vaccine vaccine NN cord-323428-jd91k19z 166 19 construction construction NN cord-323428-jd91k19z 166 20 [ [ -LRB- cord-323428-jd91k19z 166 21 13 13 CD cord-323428-jd91k19z 166 22 , , , cord-323428-jd91k19z 166 23 14 14 CD cord-323428-jd91k19z 166 24 , , , cord-323428-jd91k19z 166 25 17 17 CD cord-323428-jd91k19z 166 26 , , , cord-323428-jd91k19z 166 27 [ [ -LRB- cord-323428-jd91k19z 166 28 19 19 CD cord-323428-jd91k19z 166 29 ] ] -RRB- cord-323428-jd91k19z 166 30 [ [ -LRB- cord-323428-jd91k19z 166 31 20 20 CD cord-323428-jd91k19z 166 32 ] ] -RRB- cord-323428-jd91k19z 166 33 [ [ -LRB- cord-323428-jd91k19z 166 34 21 21 CD cord-323428-jd91k19z 166 35 ] ] -RRB- cord-323428-jd91k19z 166 36 [ [ -LRB- cord-323428-jd91k19z 166 37 22 22 CD cord-323428-jd91k19z 166 38 ] ] -RRB- cord-323428-jd91k19z 166 39 [ [ -LRB- cord-323428-jd91k19z 166 40 23 23 CD cord-323428-jd91k19z 166 41 ] ] -RRB- cord-323428-jd91k19z 166 42 . . . cord-323428-jd91k19z 167 1 The the DT cord-323428-jd91k19z 167 2 T T NNP cord-323428-jd91k19z 167 3 helper helper NN cord-323428-jd91k19z 167 4 1 1 CD cord-323428-jd91k19z 167 5 immune immune JJ cord-323428-jd91k19z 167 6 response response NN cord-323428-jd91k19z 167 7 is be VBZ cord-323428-jd91k19z 167 8 known know VBN cord-323428-jd91k19z 167 9 as as IN cord-323428-jd91k19z 167 10 the the DT cord-323428-jd91k19z 167 11 dominant dominant JJ cord-323428-jd91k19z 167 12 immune immune JJ cord-323428-jd91k19z 167 13 response response NN cord-323428-jd91k19z 167 14 in in IN cord-323428-jd91k19z 167 15 the the DT cord-323428-jd91k19z 167 16 case case NN cord-323428-jd91k19z 167 17 of of IN cord-323428-jd91k19z 167 18 intracellular intracellular JJ cord-323428-jd91k19z 167 19 pathogens pathogen NNS cord-323428-jd91k19z 167 20 , , , cord-323428-jd91k19z 167 21 such such JJ cord-323428-jd91k19z 167 22 as as IN cord-323428-jd91k19z 167 23 viruses virus NNS cord-323428-jd91k19z 167 24 and and CC cord-323428-jd91k19z 167 25 bacteria bacteria NNS cord-323428-jd91k19z 167 26 , , , cord-323428-jd91k19z 167 27 while while IN cord-323428-jd91k19z 167 28 the the DT cord-323428-jd91k19z 167 29 T T NNP cord-323428-jd91k19z 167 30 helper helper NN cord-323428-jd91k19z 167 31 2 2 CD cord-323428-jd91k19z 167 32 immune immune JJ cord-323428-jd91k19z 167 33 response response NN cord-323428-jd91k19z 167 34 is be VBZ cord-323428-jd91k19z 167 35 the the DT cord-323428-jd91k19z 167 36 dominant dominant JJ cord-323428-jd91k19z 167 37 immune immune JJ cord-323428-jd91k19z 167 38 response response NN cord-323428-jd91k19z 167 39 in in IN cord-323428-jd91k19z 167 40 cases case NNS cord-323428-jd91k19z 167 41 of of IN cord-323428-jd91k19z 167 42 extracellular extracellular JJ cord-323428-jd91k19z 167 43 pathogens pathogen NNS cord-323428-jd91k19z 167 44 and and CC cord-323428-jd91k19z 167 45 allergic allergic JJ cord-323428-jd91k19z 167 46 reactions reaction NNS cord-323428-jd91k19z 167 47 [ [ -LRB- cord-323428-jd91k19z 167 48 41 41 CD cord-323428-jd91k19z 167 49 ] ] -RRB- cord-323428-jd91k19z 167 50 . . . cord-323428-jd91k19z 168 1 Many many JJ cord-323428-jd91k19z 168 2 pathogens pathogen NNS cord-323428-jd91k19z 168 3 , , , cord-323428-jd91k19z 168 4 especially especially RB cord-323428-jd91k19z 168 5 viruses virus NNS cord-323428-jd91k19z 168 6 , , , cord-323428-jd91k19z 168 7 shift shift VB cord-323428-jd91k19z 168 8 the the DT cord-323428-jd91k19z 168 9 host host NN cord-323428-jd91k19z 168 10 immune immune JJ cord-323428-jd91k19z 168 11 response response NN cord-323428-jd91k19z 168 12 toward toward IN cord-323428-jd91k19z 168 13 Th2 th2 CD cord-323428-jd91k19z 168 14 dominance dominance NN cord-323428-jd91k19z 168 15 over over IN cord-323428-jd91k19z 168 16 the the DT cord-323428-jd91k19z 168 17 Th1 th1 CD cord-323428-jd91k19z 168 18 response response NN cord-323428-jd91k19z 168 19 to to TO cord-323428-jd91k19z 168 20 evade evade VB cord-323428-jd91k19z 168 21 cellular cellular JJ cord-323428-jd91k19z 168 22 immunity immunity NN cord-323428-jd91k19z 168 23 . . . cord-323428-jd91k19z 169 1 In in IN cord-323428-jd91k19z 169 2 this this DT cord-323428-jd91k19z 169 3 study study NN cord-323428-jd91k19z 169 4 , , , cord-323428-jd91k19z 169 5 Ad ad NN cord-323428-jd91k19z 169 6 - - HYPH cord-323428-jd91k19z 169 7 MERS MERS NNP cord-323428-jd91k19z 169 8 - - HYPH cord-323428-jd91k19z 169 9 S1 S1 NNP cord-323428-jd91k19z 169 10 was be VBD cord-323428-jd91k19z 169 11 able able JJ cord-323428-jd91k19z 169 12 to to TO cord-323428-jd91k19z 169 13 provoke provoke VB cord-323428-jd91k19z 169 14 pro pro JJ cord-323428-jd91k19z 169 15 - - JJ cord-323428-jd91k19z 169 16 inflammatory inflammatory JJ cord-323428-jd91k19z 169 17 but but CC cord-323428-jd91k19z 169 18 not not RB cord-323428-jd91k19z 169 19 anti anti JJ cord-323428-jd91k19z 169 20 - - JJ cord-323428-jd91k19z 169 21 inflammatory inflammatory JJ cord-323428-jd91k19z 169 22 cytokine cytokine NN cord-323428-jd91k19z 169 23 release release NN cord-323428-jd91k19z 169 24 . . . cord-323428-jd91k19z 170 1 Both both DT cord-323428-jd91k19z 170 2 Th1 th1 NN cord-323428-jd91k19z 170 3 and and CC cord-323428-jd91k19z 170 4 Th2 th2 CD cord-323428-jd91k19z 170 5 responses response NNS cord-323428-jd91k19z 170 6 were be VBD cord-323428-jd91k19z 170 7 elicited elicit VBN cord-323428-jd91k19z 170 8 following follow VBG cord-323428-jd91k19z 170 9 immunization immunization NN cord-323428-jd91k19z 170 10 . . . cord-323428-jd91k19z 171 1 Cytokines cytokine NNS cord-323428-jd91k19z 171 2 such such JJ cord-323428-jd91k19z 171 3 as as IN cord-323428-jd91k19z 171 4 IFN IFN NNP cord-323428-jd91k19z 171 5 - - HYPH cord-323428-jd91k19z 171 6 γ γ NNP cord-323428-jd91k19z 171 7 and and CC cord-323428-jd91k19z 171 8 IL-12 IL-12 NNP cord-323428-jd91k19z 171 9 , , , cord-323428-jd91k19z 171 10 which which WDT cord-323428-jd91k19z 171 11 represent represent VBP cord-323428-jd91k19z 171 12 the the DT cord-323428-jd91k19z 171 13 Th-1 Th-1 NNP cord-323428-jd91k19z 171 14 response response NN cord-323428-jd91k19z 171 15 , , , cord-323428-jd91k19z 171 16 were be VBD cord-323428-jd91k19z 171 17 upregulated upregulate VBN cord-323428-jd91k19z 171 18 , , , cord-323428-jd91k19z 171 19 while while IN cord-323428-jd91k19z 171 20 IL-4 IL-4 NNP cord-323428-jd91k19z 171 21 , , , cord-323428-jd91k19z 171 22 which which WDT cord-323428-jd91k19z 171 23 represents represent VBZ cord-323428-jd91k19z 171 24 the the DT cord-323428-jd91k19z 171 25 Th-2 Th-2 NNP cord-323428-jd91k19z 171 26 response response NN cord-323428-jd91k19z 171 27 , , , cord-323428-jd91k19z 171 28 was be VBD cord-323428-jd91k19z 171 29 downregulated downregulate VBN cord-323428-jd91k19z 171 30 . . . cord-323428-jd91k19z 172 1 The the DT cord-323428-jd91k19z 172 2 Ad ad NN cord-323428-jd91k19z 172 3 - - HYPH cord-323428-jd91k19z 172 4 MERS MERS NNP cord-323428-jd91k19z 172 5 - - HYPH cord-323428-jd91k19z 172 6 S1 S1 NNP cord-323428-jd91k19z 172 7 was be VBD cord-323428-jd91k19z 172 8 able able JJ cord-323428-jd91k19z 172 9 to to TO cord-323428-jd91k19z 172 10 induce induce VB cord-323428-jd91k19z 172 11 production production NN cord-323428-jd91k19z 172 12 of of IN cord-323428-jd91k19z 172 13 a a DT cord-323428-jd91k19z 172 14 significant significant JJ cord-323428-jd91k19z 172 15 amount amount NN cord-323428-jd91k19z 172 16 of of IN cord-323428-jd91k19z 172 17 IFN IFN NNP cord-323428-jd91k19z 172 18 - - HYPH cord-323428-jd91k19z 172 19 γ γ NNP cord-323428-jd91k19z 172 20 regardless regardless RB cord-323428-jd91k19z 172 21 of of IN cord-323428-jd91k19z 172 22 antigen antigen NN cord-323428-jd91k19z 172 23 - - HYPH cord-323428-jd91k19z 172 24 specific specific JJ cord-323428-jd91k19z 172 25 stimulation stimulation NN cord-323428-jd91k19z 172 26 . . . cord-323428-jd91k19z 173 1 A a DT cord-323428-jd91k19z 173 2 similar similar JJ cord-323428-jd91k19z 173 3 increase increase NN cord-323428-jd91k19z 173 4 in in IN cord-323428-jd91k19z 173 5 IL-12 IL-12 NNP cord-323428-jd91k19z 173 6 was be VBD cord-323428-jd91k19z 173 7 apparent apparent JJ cord-323428-jd91k19z 173 8 in in IN cord-323428-jd91k19z 173 9 vaccinated vaccinated JJ cord-323428-jd91k19z 173 10 animals animal NNS cord-323428-jd91k19z 173 11 in in IN cord-323428-jd91k19z 173 12 response response NN cord-323428-jd91k19z 173 13 to to IN cord-323428-jd91k19z 173 14 specific specific JJ cord-323428-jd91k19z 173 15 in in IN cord-323428-jd91k19z 173 16 vitro vitro FW cord-323428-jd91k19z 173 17 antigen antigen NN cord-323428-jd91k19z 173 18 stimulation stimulation NN cord-323428-jd91k19z 173 19 . . . cord-323428-jd91k19z 174 1 The the DT cord-323428-jd91k19z 174 2 mRNA mRNA NNP cord-323428-jd91k19z 174 3 expression expression NN cord-323428-jd91k19z 174 4 of of IN cord-323428-jd91k19z 174 5 IFN IFN NNP cord-323428-jd91k19z 174 6 - - HYPH cord-323428-jd91k19z 174 7 γ γ NNP cord-323428-jd91k19z 174 8 was be VBD cord-323428-jd91k19z 174 9 detected detect VBN cord-323428-jd91k19z 174 10 as as RB cord-323428-jd91k19z 174 11 early early RB cord-323428-jd91k19z 174 12 as as IN cord-323428-jd91k19z 174 13 4 4 CD cord-323428-jd91k19z 174 14 weeks week NNS cord-323428-jd91k19z 174 15 after after IN cord-323428-jd91k19z 174 16 the the DT cord-323428-jd91k19z 174 17 first first JJ cord-323428-jd91k19z 174 18 immunization immunization NN cord-323428-jd91k19z 174 19 . . . cord-323428-jd91k19z 175 1 In in IN cord-323428-jd91k19z 175 2 contrast contrast NN cord-323428-jd91k19z 175 3 , , , cord-323428-jd91k19z 175 4 IL-4 IL-4 NNP cord-323428-jd91k19z 175 5 production production NN cord-323428-jd91k19z 175 6 in in IN cord-323428-jd91k19z 175 7 cell cell NN cord-323428-jd91k19z 175 8 culture culture NN cord-323428-jd91k19z 175 9 showed show VBD cord-323428-jd91k19z 175 10 a a DT cord-323428-jd91k19z 175 11 significant significant JJ cord-323428-jd91k19z 175 12 increase increase NN cord-323428-jd91k19z 175 13 in in IN cord-323428-jd91k19z 175 14 control control NN cord-323428-jd91k19z 175 15 animals animal NNS cord-323428-jd91k19z 175 16 after after IN cord-323428-jd91k19z 175 17 antigen antigen NN cord-323428-jd91k19z 175 18 stimulation stimulation NN cord-323428-jd91k19z 175 19 , , , cord-323428-jd91k19z 175 20 while while IN cord-323428-jd91k19z 175 21 the the DT cord-323428-jd91k19z 175 22 production production NN cord-323428-jd91k19z 175 23 of of IN cord-323428-jd91k19z 175 24 this this DT cord-323428-jd91k19z 175 25 cytokine cytokine NN cord-323428-jd91k19z 175 26 was be VBD cord-323428-jd91k19z 175 27 significantly significantly RB cord-323428-jd91k19z 175 28 decreased decrease VBN cord-323428-jd91k19z 175 29 in in IN cord-323428-jd91k19z 175 30 the the DT cord-323428-jd91k19z 175 31 At at IN cord-323428-jd91k19z 175 32 week week NN cord-323428-jd91k19z 175 33 4 4 CD cord-323428-jd91k19z 175 34 , , , cord-323428-jd91k19z 175 35 the the DT cord-323428-jd91k19z 175 36 fold fold JJ cord-323428-jd91k19z 175 37 change change NN cord-323428-jd91k19z 175 38 in in IN cord-323428-jd91k19z 175 39 IFN IFN NNP cord-323428-jd91k19z 175 40 - - HYPH cord-323428-jd91k19z 175 41 γ γ NN cord-323428-jd91k19z 175 42 gene gene NN cord-323428-jd91k19z 175 43 expression expression NN cord-323428-jd91k19z 175 44 was be VBD cord-323428-jd91k19z 175 45 significantly significantly RB cord-323428-jd91k19z 175 46 higher high JJR cord-323428-jd91k19z 175 47 in in IN cord-323428-jd91k19z 175 48 the the DT cord-323428-jd91k19z 175 49 Ad Ad NNP cord-323428-jd91k19z 175 50 - - HYPH cord-323428-jd91k19z 175 51 Middle Middle NNP cord-323428-jd91k19z 175 52 East East NNP cord-323428-jd91k19z 175 53 respiratory respiratory JJ cord-323428-jd91k19z 175 54 syndrome syndrome NN cord-323428-jd91k19z 175 55 ( ( -LRB- cord-323428-jd91k19z 175 56 MERS)-S1 MERS)-S1 NNS cord-323428-jd91k19z 175 57 group group NN cord-323428-jd91k19z 175 58 than than IN cord-323428-jd91k19z 175 59 that that DT cord-323428-jd91k19z 175 60 of of IN cord-323428-jd91k19z 175 61 both both DT cord-323428-jd91k19z 175 62 control control NN cord-323428-jd91k19z 175 63 groups group NNS cord-323428-jd91k19z 175 64 ( ( -LRB- cord-323428-jd91k19z 175 65 Ad ad NN cord-323428-jd91k19z 175 66 - - HYPH cord-323428-jd91k19z 175 67 cytomegalovirus cytomegalovirus NN cord-323428-jd91k19z 175 68 [ [ -LRB- cord-323428-jd91k19z 175 69 CMV CMV NNP cord-323428-jd91k19z 175 70 ] ] -RRB- cord-323428-jd91k19z 175 71 and and CC cord-323428-jd91k19z 175 72 phosphate phosphate NN cord-323428-jd91k19z 175 73 - - HYPH cord-323428-jd91k19z 175 74 buffered buffer VBN cord-323428-jd91k19z 175 75 saline saline NN cord-323428-jd91k19z 175 76 ) ) -RRB- cord-323428-jd91k19z 175 77 . . . cord-323428-jd91k19z 176 1 However however RB cord-323428-jd91k19z 176 2 , , , cord-323428-jd91k19z 176 3 there there EX cord-323428-jd91k19z 176 4 was be VBD cord-323428-jd91k19z 176 5 a a DT cord-323428-jd91k19z 176 6 prominent prominent JJ cord-323428-jd91k19z 176 7 increase increase NN cord-323428-jd91k19z 176 8 in in IN cord-323428-jd91k19z 176 9 IFN IFN NNP cord-323428-jd91k19z 176 10 - - HYPH cord-323428-jd91k19z 176 11 γ γ NN cord-323428-jd91k19z 176 12 gene gene NN cord-323428-jd91k19z 176 13 expression expression NN cord-323428-jd91k19z 176 14 at at IN cord-323428-jd91k19z 176 15 week week NN cord-323428-jd91k19z 176 16 6 6 CD cord-323428-jd91k19z 176 17 in in IN cord-323428-jd91k19z 176 18 both both CC cord-323428-jd91k19z 176 19 Ad ad NN cord-323428-jd91k19z 176 20 - - HYPH cord-323428-jd91k19z 176 21 MERS MERS NNP cord-323428-jd91k19z 176 22 - - HYPH cord-323428-jd91k19z 176 23 S1 S1 NNP cord-323428-jd91k19z 176 24 and and CC cord-323428-jd91k19z 176 25 Ad Ad NNP cord-323428-jd91k19z 176 26 - - HYPH cord-323428-jd91k19z 176 27 CMV CMV NNP cord-323428-jd91k19z 176 28 groups group NNS cord-323428-jd91k19z 176 29 compared compare VBN cord-323428-jd91k19z 176 30 to to IN cord-323428-jd91k19z 176 31 that that DT cord-323428-jd91k19z 176 32 in in IN cord-323428-jd91k19z 176 33 week week NN cord-323428-jd91k19z 176 34 4 4 CD cord-323428-jd91k19z 176 35 . . . cord-323428-jd91k19z 177 1 The the DT cord-323428-jd91k19z 177 2 only only JJ cord-323428-jd91k19z 177 3 significant significant JJ cord-323428-jd91k19z 177 4 change change NN cord-323428-jd91k19z 177 5 in in IN cord-323428-jd91k19z 177 6 IL-4 IL-4 NNP cord-323428-jd91k19z 177 7 gene gene NN cord-323428-jd91k19z 177 8 expression expression NN cord-323428-jd91k19z 177 9 was be VBD cord-323428-jd91k19z 177 10 in in IN cord-323428-jd91k19z 177 11 the the DT cord-323428-jd91k19z 177 12 Ad Ad NNP cord-323428-jd91k19z 177 13 - - HYPH cord-323428-jd91k19z 177 14 CMV CMV NNP cord-323428-jd91k19z 177 15 group group NN cord-323428-jd91k19z 177 16 at at IN cord-323428-jd91k19z 177 17 week week NN cord-323428-jd91k19z 177 18 6 6 CD cord-323428-jd91k19z 177 19 , , , cord-323428-jd91k19z 177 20 while while IN cord-323428-jd91k19z 177 21 no no DT cord-323428-jd91k19z 177 22 significant significant JJ cord-323428-jd91k19z 177 23 changes change NNS cord-323428-jd91k19z 177 24 were be VBD cord-323428-jd91k19z 177 25 observed observe VBN cord-323428-jd91k19z 177 26 in in IN cord-323428-jd91k19z 177 27 the the DT cord-323428-jd91k19z 177 28 Ad ad NN cord-323428-jd91k19z 177 29 - - HYPH cord-323428-jd91k19z 177 30 MERS MERS NNP cord-323428-jd91k19z 177 31 - - HYPH cord-323428-jd91k19z 177 32 S1 S1 NNP cord-323428-jd91k19z 177 33 group group NN cord-323428-jd91k19z 177 34 at at IN cord-323428-jd91k19z 177 35 any any DT cord-323428-jd91k19z 177 36 time time NN cord-323428-jd91k19z 177 37 point point NN cord-323428-jd91k19z 177 38 . . . cord-323428-jd91k19z 178 1 Different different JJ cord-323428-jd91k19z 178 2 letters letter NNS cord-323428-jd91k19z 178 3 indicate indicate VBP cord-323428-jd91k19z 178 4 statistically statistically RB cord-323428-jd91k19z 178 5 significant significant JJ cord-323428-jd91k19z 178 6 differences difference NNS cord-323428-jd91k19z 178 7 at at IN cord-323428-jd91k19z 178 8 p<0.05 p<0.05 CD cord-323428-jd91k19z 178 9 . . . cord-323428-jd91k19z 179 1 vaccinated vaccinated JJ cord-323428-jd91k19z 179 2 group group NN cord-323428-jd91k19z 179 3 . . . cord-323428-jd91k19z 180 1 The the DT cord-323428-jd91k19z 180 2 results result NNS cord-323428-jd91k19z 180 3 demonstrated demonstrate VBD cord-323428-jd91k19z 180 4 neither neither CC cord-323428-jd91k19z 180 5 positive positive JJ cord-323428-jd91k19z 180 6 nor nor CC cord-323428-jd91k19z 180 7 negative negative JJ cord-323428-jd91k19z 180 8 impact impact NN cord-323428-jd91k19z 180 9 on on IN cord-323428-jd91k19z 180 10 IL-4 IL-4 NNP cord-323428-jd91k19z 180 11 expression expression NN cord-323428-jd91k19z 180 12 in in IN cord-323428-jd91k19z 180 13 mice mouse NNS cord-323428-jd91k19z 180 14 vaccinated vaccinate VBN cord-323428-jd91k19z 180 15 with with IN cord-323428-jd91k19z 180 16 the the DT cord-323428-jd91k19z 180 17 Ad ad NN cord-323428-jd91k19z 180 18 - - HYPH cord-323428-jd91k19z 180 19 MERS MERS NNP cord-323428-jd91k19z 180 20 - - HYPH cord-323428-jd91k19z 180 21 S1 S1 NNP cord-323428-jd91k19z 180 22 or or CC cord-323428-jd91k19z 180 23 mice mouse NNS cord-323428-jd91k19z 180 24 that that WDT cord-323428-jd91k19z 180 25 received receive VBD cord-323428-jd91k19z 180 26 only only RB cord-323428-jd91k19z 180 27 PBS PBS NNP cord-323428-jd91k19z 180 28 . . . cord-323428-jd91k19z 181 1 Interestingly interestingly RB cord-323428-jd91k19z 181 2 , , , cord-323428-jd91k19z 181 3 there there EX cord-323428-jd91k19z 181 4 was be VBD cord-323428-jd91k19z 181 5 a a DT cord-323428-jd91k19z 181 6 significant significant JJ cord-323428-jd91k19z 181 7 increase increase NN cord-323428-jd91k19z 181 8 in in IN cord-323428-jd91k19z 181 9 IL-4 IL-4 NNP cord-323428-jd91k19z 181 10 and and CC cord-323428-jd91k19z 181 11 IFN IFN NNP cord-323428-jd91k19z 181 12 - - HYPH cord-323428-jd91k19z 181 13 γ γ NN cord-323428-jd91k19z 181 14 gene gene NN cord-323428-jd91k19z 181 15 expression expression NN cord-323428-jd91k19z 181 16 in in IN cord-323428-jd91k19z 181 17 antigen antigen NN cord-323428-jd91k19z 181 18 - - HYPH cord-323428-jd91k19z 181 19 stimulated stimulate VBN cord-323428-jd91k19z 181 20 cell cell NN cord-323428-jd91k19z 181 21 culture culture NN cord-323428-jd91k19z 181 22 obtained obtain VBN cord-323428-jd91k19z 181 23 from from IN cord-323428-jd91k19z 181 24 mice mouse NNS cord-323428-jd91k19z 181 25 vaccinated vaccinate VBN cord-323428-jd91k19z 181 26 with with IN cord-323428-jd91k19z 181 27 Ad Ad NNP cord-323428-jd91k19z 181 28 - - HYPH cord-323428-jd91k19z 181 29 CMV CMV NNP cord-323428-jd91k19z 181 30 at at IN cord-323428-jd91k19z 181 31 week week NN cord-323428-jd91k19z 181 32 6 6 CD cord-323428-jd91k19z 181 33 . . . cord-323428-jd91k19z 182 1 This this DT cord-323428-jd91k19z 182 2 might may MD cord-323428-jd91k19z 182 3 be be VB cord-323428-jd91k19z 182 4 due due JJ cord-323428-jd91k19z 182 5 to to IN cord-323428-jd91k19z 182 6 the the DT cord-323428-jd91k19z 182 7 ability ability NN cord-323428-jd91k19z 182 8 of of IN cord-323428-jd91k19z 182 9 adenoviruses adenovirus NNS cord-323428-jd91k19z 182 10 to to TO cord-323428-jd91k19z 182 11 elicit elicit VB cord-323428-jd91k19z 182 12 strong strong JJ cord-323428-jd91k19z 182 13 humoral humoral JJ cord-323428-jd91k19z 182 14 and and CC cord-323428-jd91k19z 182 15 cellular cellular JJ cord-323428-jd91k19z 182 16 immune immune JJ cord-323428-jd91k19z 182 17 responses response NNS cord-323428-jd91k19z 182 18 [ [ -LRB- cord-323428-jd91k19z 182 19 42 42 CD cord-323428-jd91k19z 182 20 , , , cord-323428-jd91k19z 182 21 43 43 CD cord-323428-jd91k19z 182 22 ] ] -RRB- cord-323428-jd91k19z 182 23 . . . cord-323428-jd91k19z 183 1 Therefore therefore RB cord-323428-jd91k19z 183 2 , , , cord-323428-jd91k19z 183 3 it -PRON- PRP cord-323428-jd91k19z 183 4 can can MD cord-323428-jd91k19z 183 5 be be VB cord-323428-jd91k19z 183 6 concluded conclude VBN cord-323428-jd91k19z 183 7 that that IN cord-323428-jd91k19z 183 8 the the DT cord-323428-jd91k19z 183 9 presence presence NN cord-323428-jd91k19z 183 10 of of IN cord-323428-jd91k19z 183 11 the the DT cord-323428-jd91k19z 183 12 S1 S1 NNP cord-323428-jd91k19z 183 13 protein protein NN cord-323428-jd91k19z 183 14 gene gene NN cord-323428-jd91k19z 183 15 in in IN cord-323428-jd91k19z 183 16 the the DT cord-323428-jd91k19z 183 17 adenovirus adenovirus NN cord-323428-jd91k19z 183 18 vector vector NN cord-323428-jd91k19z 183 19 genome genome NN cord-323428-jd91k19z 183 20 disturbs disturb VBZ cord-323428-jd91k19z 183 21 the the DT cord-323428-jd91k19z 183 22 immune immune JJ cord-323428-jd91k19z 183 23 stimulation stimulation NN cord-323428-jd91k19z 183 24 ability ability NN cord-323428-jd91k19z 183 25 of of IN cord-323428-jd91k19z 183 26 the the DT cord-323428-jd91k19z 183 27 adenovirus adenovirus NN cord-323428-jd91k19z 183 28 vector vector NN cord-323428-jd91k19z 183 29 to to TO cord-323428-jd91k19z 183 30 elicit elicit VB cord-323428-jd91k19z 183 31 IL-4 IL-4 NNP cord-323428-jd91k19z 183 32 cytokine cytokine NN cord-323428-jd91k19z 183 33 . . . cord-323428-jd91k19z 184 1 Two two CD cord-323428-jd91k19z 184 2 studies study NNS cord-323428-jd91k19z 184 3 have have VBP cord-323428-jd91k19z 184 4 shed shed VBN cord-323428-jd91k19z 184 5 light light NN cord-323428-jd91k19z 184 6 on on IN cord-323428-jd91k19z 184 7 the the DT cord-323428-jd91k19z 184 8 ability ability NN cord-323428-jd91k19z 184 9 of of IN cord-323428-jd91k19z 184 10 the the DT cord-323428-jd91k19z 184 11 S1 S1 NNP cord-323428-jd91k19z 184 12 subunit subunit NN cord-323428-jd91k19z 184 13 , , , cord-323428-jd91k19z 184 14 and and CC cord-323428-jd91k19z 184 15 specifically specifically RB cord-323428-jd91k19z 184 16 , , , cord-323428-jd91k19z 184 17 the the DT cord-323428-jd91k19z 184 18 ability ability NN cord-323428-jd91k19z 184 19 of of IN cord-323428-jd91k19z 184 20 the the DT cord-323428-jd91k19z 184 21 RBD RBD NNP cord-323428-jd91k19z 184 22 domain domain NN cord-323428-jd91k19z 184 23 , , , cord-323428-jd91k19z 184 24 to to TO cord-323428-jd91k19z 184 25 shift shift VB cord-323428-jd91k19z 184 26 the the DT cord-323428-jd91k19z 184 27 host host NN cord-323428-jd91k19z 184 28 immune immune JJ cord-323428-jd91k19z 184 29 response response NN cord-323428-jd91k19z 184 30 toward toward IN cord-323428-jd91k19z 184 31 Th1 th1 CD cord-323428-jd91k19z 184 32 [ [ -LRB- cord-323428-jd91k19z 184 33 14 14 CD cord-323428-jd91k19z 184 34 , , , cord-323428-jd91k19z 184 35 44 44 CD cord-323428-jd91k19z 184 36 ] ] -RRB- cord-323428-jd91k19z 184 37 . . . cord-323428-jd91k19z 185 1 It -PRON- PRP cord-323428-jd91k19z 185 2 was be VBD cord-323428-jd91k19z 185 3 demonstrated demonstrate VBN cord-323428-jd91k19z 185 4 in in IN cord-323428-jd91k19z 185 5 these these DT cord-323428-jd91k19z 185 6 two two CD cord-323428-jd91k19z 185 7 studies study NNS cord-323428-jd91k19z 185 8 that that WDT cord-323428-jd91k19z 185 9 IgG1 IgG1 NNP cord-323428-jd91k19z 185 10 , , , cord-323428-jd91k19z 185 11 which which WDT cord-323428-jd91k19z 185 12 is be VBZ cord-323428-jd91k19z 185 13 produced produce VBN cord-323428-jd91k19z 185 14 mainly mainly RB cord-323428-jd91k19z 185 15 during during IN cord-323428-jd91k19z 185 16 the the DT cord-323428-jd91k19z 185 17 Th2 Th2 NNP cord-323428-jd91k19z 185 18 immune immune JJ cord-323428-jd91k19z 185 19 response response NN cord-323428-jd91k19z 185 20 , , , cord-323428-jd91k19z 185 21 was be VBD cord-323428-jd91k19z 185 22 decreased decrease VBN cord-323428-jd91k19z 185 23 , , , cord-323428-jd91k19z 185 24 while while IN cord-323428-jd91k19z 185 25 IgG2a IgG2a NNP cord-323428-jd91k19z 185 26 , , , cord-323428-jd91k19z 185 27 which which WDT cord-323428-jd91k19z 185 28 is be VBZ cord-323428-jd91k19z 185 29 produced produce VBN cord-323428-jd91k19z 185 30 mainly mainly RB cord-323428-jd91k19z 185 31 during during IN cord-323428-jd91k19z 185 32 the the DT cord-323428-jd91k19z 185 33 Th1 th1 CD cord-323428-jd91k19z 185 34 immune immune JJ cord-323428-jd91k19z 185 35 response response NN cord-323428-jd91k19z 185 36 , , , cord-323428-jd91k19z 185 37 was be VBD cord-323428-jd91k19z 185 38 upregulated upregulate VBN cord-323428-jd91k19z 185 39 following follow VBG cord-323428-jd91k19z 185 40 vaccination vaccination NN cord-323428-jd91k19z 185 41 with with IN cord-323428-jd91k19z 185 42 their -PRON- PRP$ cord-323428-jd91k19z 185 43 vaccine vaccine NN cord-323428-jd91k19z 185 44 candidate candidate NN cord-323428-jd91k19z 185 45 . . . cord-323428-jd91k19z 186 1 The the DT cord-323428-jd91k19z 186 2 truncated truncated JJ cord-323428-jd91k19z 186 3 RBD RBD NNP cord-323428-jd91k19z 186 4 fused fuse VBN cord-323428-jd91k19z 186 5 with with IN cord-323428-jd91k19z 186 6 the the DT cord-323428-jd91k19z 186 7 Fc Fc NNP cord-323428-jd91k19z 186 8 portion portion NN cord-323428-jd91k19z 186 9 of of IN cord-323428-jd91k19z 186 10 human human JJ cord-323428-jd91k19z 186 11 IgG igg NN cord-323428-jd91k19z 186 12 was be VBD cord-323428-jd91k19z 186 13 able able JJ cord-323428-jd91k19z 186 14 to to TO cord-323428-jd91k19z 186 15 elicit elicit VB cord-323428-jd91k19z 186 16 both both DT cord-323428-jd91k19z 186 17 isotypes isotype NNS cord-323428-jd91k19z 186 18 with with IN cord-323428-jd91k19z 186 19 a a DT cord-323428-jd91k19z 186 20 relatively relatively RB cord-323428-jd91k19z 186 21 higher high JJR cord-323428-jd91k19z 186 22 amount amount NN cord-323428-jd91k19z 186 23 of of IN cord-323428-jd91k19z 186 24 the the DT cord-323428-jd91k19z 186 25 IgG2a IgG2a NNP cord-323428-jd91k19z 186 26 isotype isotype NN cord-323428-jd91k19z 186 27 ( ( -LRB- cord-323428-jd91k19z 186 28 Th1 th1 CD cord-323428-jd91k19z 186 29 response response NN cord-323428-jd91k19z 186 30 ) ) -RRB- cord-323428-jd91k19z 186 31 [ [ -LRB- cord-323428-jd91k19z 186 32 44 44 CD cord-323428-jd91k19z 186 33 ] ] -RRB- cord-323428-jd91k19z 186 34 . . . cord-323428-jd91k19z 187 1 Recombinant recombinant JJ cord-323428-jd91k19z 187 2 adenovirus adenovirus NN cord-323428-jd91k19z 187 3 vector vector NN cord-323428-jd91k19z 187 4 carrying carry VBG cord-323428-jd91k19z 187 5 the the DT cord-323428-jd91k19z 187 6 S1 S1 NNP cord-323428-jd91k19z 187 7 subunit subunit NN cord-323428-jd91k19z 187 8 or or CC cord-323428-jd91k19z 187 9 the the DT cord-323428-jd91k19z 187 10 whole whole JJ cord-323428-jd91k19z 187 11 S S NNP cord-323428-jd91k19z 187 12 gene gene NN cord-323428-jd91k19z 187 13 was be VBD cord-323428-jd91k19z 187 14 also also RB cord-323428-jd91k19z 187 15 able able JJ cord-323428-jd91k19z 187 16 to to TO cord-323428-jd91k19z 187 17 induce induce VB cord-323428-jd91k19z 187 18 both both DT cord-323428-jd91k19z 187 19 types type NNS cord-323428-jd91k19z 187 20 of of IN cord-323428-jd91k19z 187 21 the the DT cord-323428-jd91k19z 187 22 immune immune JJ cord-323428-jd91k19z 187 23 response response NN cord-323428-jd91k19z 187 24 . . . cord-323428-jd91k19z 188 1 The the DT cord-323428-jd91k19z 188 2 IgG2a IgG2a NNP cord-323428-jd91k19z 188 3 level level NN cord-323428-jd91k19z 188 4 was be VBD cord-323428-jd91k19z 188 5 detected detect VBN cord-323428-jd91k19z 188 6 earlier early RBR cord-323428-jd91k19z 188 7 and and CC cord-323428-jd91k19z 188 8 higher high JJR cord-323428-jd91k19z 188 9 in in IN cord-323428-jd91k19z 188 10 MERS MERS NNP cord-323428-jd91k19z 188 11 - - HYPH cord-323428-jd91k19z 188 12 S S NNP cord-323428-jd91k19z 188 13 than than IN cord-323428-jd91k19z 188 14 MERS MERS NNP cord-323428-jd91k19z 188 15 - - HYPH cord-323428-jd91k19z 188 16 S1 S1 NNP cord-323428-jd91k19z 188 17 at at IN cord-323428-jd91k19z 188 18 week week NN cord-323428-jd91k19z 188 19 2 2 CD cord-323428-jd91k19z 188 20 post post NN cord-323428-jd91k19z 188 21 - - NN cord-323428-jd91k19z 188 22 vaccination vaccination NN cord-323428-jd91k19z 189 1 but but CC cord-323428-jd91k19z 189 2 not not RB cord-323428-jd91k19z 189 3 after after IN cord-323428-jd91k19z 189 4 week week NN cord-323428-jd91k19z 189 5 3 3 CD cord-323428-jd91k19z 189 6 [ [ -LRB- cord-323428-jd91k19z 189 7 14 14 CD cord-323428-jd91k19z 189 8 ] ] -RRB- cord-323428-jd91k19z 189 9 . . . cord-323428-jd91k19z 190 1 Accordingly accordingly RB cord-323428-jd91k19z 190 2 , , , cord-323428-jd91k19z 190 3 these these DT cord-323428-jd91k19z 190 4 findings finding NNS cord-323428-jd91k19z 190 5 lead lead VBP cord-323428-jd91k19z 190 6 to to IN cord-323428-jd91k19z 190 7 the the DT cord-323428-jd91k19z 190 8 dominance dominance NN cord-323428-jd91k19z 190 9 toward toward IN cord-323428-jd91k19z 190 10 the the DT cord-323428-jd91k19z 190 11 Th1 th1 CD cord-323428-jd91k19z 190 12 cellular cellular JJ cord-323428-jd91k19z 190 13 immunity immunity NN cord-323428-jd91k19z 190 14 . . . cord-323428-jd91k19z 191 1 Several several JJ cord-323428-jd91k19z 191 2 in in IN cord-323428-jd91k19z 191 3 vivo vivo NN cord-323428-jd91k19z 191 4 and and CC cord-323428-jd91k19z 191 5 in in IN cord-323428-jd91k19z 191 6 vitro vitro FW cord-323428-jd91k19z 191 7 studies study NNS cord-323428-jd91k19z 191 8 have have VBP cord-323428-jd91k19z 191 9 revealed reveal VBN cord-323428-jd91k19z 191 10 the the DT cord-323428-jd91k19z 191 11 ability ability NN cord-323428-jd91k19z 191 12 of of IN cord-323428-jd91k19z 191 13 MERS MERS NNP cord-323428-jd91k19z 191 14 - - HYPH cord-323428-jd91k19z 191 15 CoV CoV NNP cord-323428-jd91k19z 191 16 to to TO cord-323428-jd91k19z 191 17 evade evade VB cord-323428-jd91k19z 191 18 the the DT cord-323428-jd91k19z 191 19 host host NN cord-323428-jd91k19z 191 20 innate innate JJ cord-323428-jd91k19z 191 21 immune immune JJ cord-323428-jd91k19z 191 22 response response NN cord-323428-jd91k19z 191 23 through through IN cord-323428-jd91k19z 191 24 downregulating downregulate VBG cord-323428-jd91k19z 191 25 the the DT cord-323428-jd91k19z 191 26 antigen antigen NN cord-323428-jd91k19z 191 27 - - HYPH cord-323428-jd91k19z 191 28 presenting present VBG cord-323428-jd91k19z 191 29 pathway pathway NN cord-323428-jd91k19z 191 30 and and CC cord-323428-jd91k19z 191 31 proinflammatory proinflammatory JJ cord-323428-jd91k19z 191 32 cytokines cytokine NNS cord-323428-jd91k19z 191 33 such such JJ cord-323428-jd91k19z 191 34 as as IN cord-323428-jd91k19z 191 35 IFN IFN NNP cord-323428-jd91k19z 191 36 - - HYPH cord-323428-jd91k19z 191 37 γ γ NNP cord-323428-jd91k19z 191 38 and and CC cord-323428-jd91k19z 191 39 IL-12 IL-12 NNP cord-323428-jd91k19z 191 40 [ [ -LRB- cord-323428-jd91k19z 191 41 38 38 CD cord-323428-jd91k19z 191 42 , , , cord-323428-jd91k19z 191 43 [ [ -LRB- cord-323428-jd91k19z 191 44 45 45 CD cord-323428-jd91k19z 191 45 ] ] -RRB- cord-323428-jd91k19z 191 46 [ [ -LRB- cord-323428-jd91k19z 191 47 46 46 CD cord-323428-jd91k19z 191 48 ] ] -RRB- cord-323428-jd91k19z 191 49 [ [ -LRB- cord-323428-jd91k19z 191 50 47 47 CD cord-323428-jd91k19z 191 51 ] ] -RRB- cord-323428-jd91k19z 191 52 . . . cord-323428-jd91k19z 192 1 Several several JJ cord-323428-jd91k19z 192 2 MERS MERS NNP cord-323428-jd91k19z 192 3 - - HYPH cord-323428-jd91k19z 192 4 CoV cov NN cord-323428-jd91k19z 192 5 proteins protein NNS cord-323428-jd91k19z 192 6 were be VBD cord-323428-jd91k19z 192 7 previously previously RB cord-323428-jd91k19z 192 8 identified identify VBN cord-323428-jd91k19z 192 9 as as IN cord-323428-jd91k19z 192 10 potent potent JJ cord-323428-jd91k19z 192 11 IFN IFN NNP cord-323428-jd91k19z 192 12 antagonists antagonist NNS cord-323428-jd91k19z 192 13 , , , cord-323428-jd91k19z 192 14 including include VBG cord-323428-jd91k19z 192 15 the the DT cord-323428-jd91k19z 192 16 M M NNP cord-323428-jd91k19z 192 17 structural structural JJ cord-323428-jd91k19z 192 18 protein protein NN cord-323428-jd91k19z 192 19 and and CC cord-323428-jd91k19z 192 20 the the DT cord-323428-jd91k19z 192 21 ORF ORF NNP cord-323428-jd91k19z 192 22 4a 4a CD cord-323428-jd91k19z 192 23 , , , cord-323428-jd91k19z 192 24 ORF ORF NNP cord-323428-jd91k19z 192 25 4b 4b NNS cord-323428-jd91k19z 192 26 , , , cord-323428-jd91k19z 192 27 and and CC cord-323428-jd91k19z 192 28 ORF ORF NNP cord-323428-jd91k19z 192 29 5 5 CD cord-323428-jd91k19z 192 30 accessory accessory NN cord-323428-jd91k19z 192 31 proteins protein NNS cord-323428-jd91k19z 192 32 [ [ -LRB- cord-323428-jd91k19z 192 33 46 46 CD cord-323428-jd91k19z 192 34 ] ] -RRB- cord-323428-jd91k19z 192 35 . . . cord-323428-jd91k19z 193 1 In in IN cord-323428-jd91k19z 193 2 addition addition NN cord-323428-jd91k19z 193 3 , , , cord-323428-jd91k19z 193 4 a a DT cord-323428-jd91k19z 193 5 comparison comparison NN cord-323428-jd91k19z 193 6 of of IN cord-323428-jd91k19z 193 7 the the DT cord-323428-jd91k19z 193 8 immune immune JJ cord-323428-jd91k19z 193 9 response response NN cord-323428-jd91k19z 193 10 of of IN cord-323428-jd91k19z 193 11 two two CD cord-323428-jd91k19z 193 12 MERS MERS NNP cord-323428-jd91k19z 193 13 - - HYPH cord-323428-jd91k19z 193 14 infected infect VBN cord-323428-jd91k19z 193 15 patients patient NNS cord-323428-jd91k19z 193 16 [ [ -LRB- cord-323428-jd91k19z 193 17 48 48 CD cord-323428-jd91k19z 193 18 ] ] -RRB- cord-323428-jd91k19z 193 19 , , , cord-323428-jd91k19z 193 20 one one CD cord-323428-jd91k19z 193 21 of of IN cord-323428-jd91k19z 193 22 whom whom WP cord-323428-jd91k19z 193 23 with with IN cord-323428-jd91k19z 193 24 a a DT cord-323428-jd91k19z 193 25 poor poor JJ cord-323428-jd91k19z 193 26 outcome outcome NN cord-323428-jd91k19z 193 27 ( ( -LRB- cord-323428-jd91k19z 193 28 died die VBD cord-323428-jd91k19z 193 29 ) ) -RRB- cord-323428-jd91k19z 193 30 that that WDT cord-323428-jd91k19z 193 31 had have VBD cord-323428-jd91k19z 193 32 elevated elevate VBN cord-323428-jd91k19z 193 33 levels level NNS cord-323428-jd91k19z 193 34 of of IN cord-323428-jd91k19z 193 35 IL-17 IL-17 NNP cord-323428-jd91k19z 193 36 and and CC cord-323428-jd91k19z 193 37 IL-10 IL-10 NNP cord-323428-jd91k19z 193 38 which which WDT cord-323428-jd91k19z 193 39 promote promote VBP cord-323428-jd91k19z 193 40 the the DT cord-323428-jd91k19z 193 41 Th2 Th2 NNP cord-323428-jd91k19z 193 42 immune immune JJ cord-323428-jd91k19z 193 43 response response NN cord-323428-jd91k19z 193 44 , , , cord-323428-jd91k19z 193 45 while while IN cord-323428-jd91k19z 193 46 the the DT cord-323428-jd91k19z 193 47 other other JJ cord-323428-jd91k19z 193 48 patient patient NN cord-323428-jd91k19z 193 49 who who WP cord-323428-jd91k19z 193 50 overcame overcome VBD cord-323428-jd91k19z 193 51 the the DT cord-323428-jd91k19z 193 52 infection infection NN cord-323428-jd91k19z 193 53 had have VBD cord-323428-jd91k19z 193 54 increased increase VBN cord-323428-jd91k19z 193 55 levels level NNS cord-323428-jd91k19z 193 56 of of IN cord-323428-jd91k19z 193 57 IFN IFN NNP cord-323428-jd91k19z 193 58 - - HYPH cord-323428-jd91k19z 193 59 α α NNP cord-323428-jd91k19z 193 60 , , , cord-323428-jd91k19z 193 61 IFN IFN NNP cord-323428-jd91k19z 193 62 - - HYPH cord-323428-jd91k19z 193 63 γ γ NNP cord-323428-jd91k19z 193 64 , , , cord-323428-jd91k19z 193 65 and and CC cord-323428-jd91k19z 193 66 IL-12 IL-12 NNP cord-323428-jd91k19z 193 67 , , , cord-323428-jd91k19z 193 68 which which WDT cord-323428-jd91k19z 193 69 promote promote VBP cord-323428-jd91k19z 193 70 the the DT cord-323428-jd91k19z 193 71 Th1 th1 CD cord-323428-jd91k19z 193 72 immune immune JJ cord-323428-jd91k19z 193 73 response response NN cord-323428-jd91k19z 193 74 [ [ -LRB- cord-323428-jd91k19z 193 75 48 48 CD cord-323428-jd91k19z 193 76 ] ] -RRB- cord-323428-jd91k19z 193 77 . . . cord-323428-jd91k19z 194 1 In in IN cord-323428-jd91k19z 194 2 general general JJ cord-323428-jd91k19z 194 3 , , , cord-323428-jd91k19z 194 4 it -PRON- PRP cord-323428-jd91k19z 194 5 is be VBZ cord-323428-jd91k19z 194 6 crucial crucial JJ cord-323428-jd91k19z 194 7 to to TO cord-323428-jd91k19z 194 8 induce induce VB cord-323428-jd91k19z 194 9 early early JJ cord-323428-jd91k19z 194 10 and and CC cord-323428-jd91k19z 194 11 strong strong JJ cord-323428-jd91k19z 194 12 innate innate JJ cord-323428-jd91k19z 194 13 immune immune JJ cord-323428-jd91k19z 194 14 responses response NNS cord-323428-jd91k19z 194 15 against against IN cord-323428-jd91k19z 194 16 MERS MERS NNP cord-323428-jd91k19z 194 17 - - HYPH cord-323428-jd91k19z 194 18 CoV cov NN cord-323428-jd91k19z 194 19 infection infection NN cord-323428-jd91k19z 194 20 to to TO cord-323428-jd91k19z 194 21 save save VB cord-323428-jd91k19z 194 22 lives life NNS cord-323428-jd91k19z 194 23 . . . cord-323428-jd91k19z 195 1 Our -PRON- PRP$ cord-323428-jd91k19z 195 2 vaccine vaccine NN cord-323428-jd91k19z 195 3 candidate candidate NN cord-323428-jd91k19z 195 4 was be VBD cord-323428-jd91k19z 195 5 able able JJ cord-323428-jd91k19z 195 6 to to TO cord-323428-jd91k19z 195 7 induce induce VB cord-323428-jd91k19z 195 8 production production NN cord-323428-jd91k19z 195 9 of of IN cord-323428-jd91k19z 195 10 the the DT cord-323428-jd91k19z 195 11 key key JJ cord-323428-jd91k19z 195 12 cytokines cytokine NNS cord-323428-jd91k19z 195 13 of of IN cord-323428-jd91k19z 195 14 activated activate VBN cord-323428-jd91k19z 195 15 T T NNP cord-323428-jd91k19z 195 16 lymphocytes lymphocyte NNS cord-323428-jd91k19z 195 17 toward toward IN cord-323428-jd91k19z 195 18 CD4 CD4 NNP cord-323428-jd91k19z 195 19 + + CC cord-323428-jd91k19z 195 20 Th1 th1 NN cord-323428-jd91k19z 195 21 cells cell NNS cord-323428-jd91k19z 195 22 , , , cord-323428-jd91k19z 195 23 which which WDT cord-323428-jd91k19z 195 24 is be VBZ cord-323428-jd91k19z 195 25 also also RB cord-323428-jd91k19z 195 26 an an DT cord-323428-jd91k19z 195 27 indication indication NN cord-323428-jd91k19z 195 28 of of IN cord-323428-jd91k19z 195 29 upregulation upregulation NN cord-323428-jd91k19z 195 30 of of IN cord-323428-jd91k19z 195 31 the the DT cord-323428-jd91k19z 195 32 antigen antigen NN cord-323428-jd91k19z 195 33 - - HYPH cord-323428-jd91k19z 195 34 presenting present VBG cord-323428-jd91k19z 195 35 pathway pathway NN cord-323428-jd91k19z 195 36 . . . cord-323428-jd91k19z 196 1 The the DT cord-323428-jd91k19z 196 2 vaccine vaccine NN cord-323428-jd91k19z 196 3 also also RB cord-323428-jd91k19z 196 4 did do VBD cord-323428-jd91k19z 196 5 not not RB cord-323428-jd91k19z 196 6 trigger trigger VB cord-323428-jd91k19z 196 7 the the DT cord-323428-jd91k19z 196 8 production production NN cord-323428-jd91k19z 196 9 of of IN cord-323428-jd91k19z 196 10 IL-4 IL-4 NNP cord-323428-jd91k19z 196 11 , , , cord-323428-jd91k19z 196 12 which which WDT cord-323428-jd91k19z 196 13 is be VBZ cord-323428-jd91k19z 196 14 involved involve VBN cord-323428-jd91k19z 196 15 in in IN cord-323428-jd91k19z 196 16 the the DT cord-323428-jd91k19z 196 17 Th2 th2 CD cord-323428-jd91k19z 196 18 response response NN cord-323428-jd91k19z 196 19 , , , cord-323428-jd91k19z 196 20 which which WDT cord-323428-jd91k19z 196 21 may may MD cord-323428-jd91k19z 196 22 be be VB cord-323428-jd91k19z 196 23 referred refer VBN cord-323428-jd91k19z 196 24 to to IN cord-323428-jd91k19z 196 25 the the DT cord-323428-jd91k19z 196 26 counter counter JJ cord-323428-jd91k19z 196 27 effect effect NN cord-323428-jd91k19z 196 28 of of IN cord-323428-jd91k19z 196 29 the the DT cord-323428-jd91k19z 196 30 produced produce VBN cord-323428-jd91k19z 196 31 IFN IFN NNP cord-323428-jd91k19z 196 32 - - HYPH cord-323428-jd91k19z 196 33 γ γ NN cord-323428-jd91k19z 196 34 in in IN cord-323428-jd91k19z 196 35 IL-4 IL-4 NNP cord-323428-jd91k19z 196 36 producing produce VBG cord-323428-jd91k19z 196 37 cells cell NNS cord-323428-jd91k19z 196 38 [ [ -LRB- cord-323428-jd91k19z 196 39 41 41 CD cord-323428-jd91k19z 196 40 ] ] -RRB- cord-323428-jd91k19z 196 41 . . . cord-323428-jd91k19z 197 1 Finally finally RB cord-323428-jd91k19z 197 2 , , , cord-323428-jd91k19z 197 3 it -PRON- PRP cord-323428-jd91k19z 197 4 is be VBZ cord-323428-jd91k19z 197 5 important important JJ cord-323428-jd91k19z 197 6 to to TO cord-323428-jd91k19z 197 7 clarify clarify VB cord-323428-jd91k19z 197 8 that that IN cord-323428-jd91k19z 197 9 the the DT cord-323428-jd91k19z 197 10 in in FW cord-323428-jd91k19z 197 11 vitro vitro FW cord-323428-jd91k19z 197 12 antigen antigen NN cord-323428-jd91k19z 197 13 stimulation stimulation NN cord-323428-jd91k19z 197 14 was be VBD cord-323428-jd91k19z 197 15 done do VBN cord-323428-jd91k19z 197 16 using use VBG cord-323428-jd91k19z 197 17 the the DT cord-323428-jd91k19z 197 18 specific specific JJ cord-323428-jd91k19z 197 19 9-mer 9-mer CD cord-323428-jd91k19z 197 20 peptide peptide NN cord-323428-jd91k19z 197 21 " " `` cord-323428-jd91k19z 197 22 CYSSLILDY CYSSLILDY NNP cord-323428-jd91k19z 197 23 , , , cord-323428-jd91k19z 197 24 " " '' cord-323428-jd91k19z 197 25 this this DT cord-323428-jd91k19z 197 26 peptide peptide NN cord-323428-jd91k19z 197 27 is be VBZ cord-323428-jd91k19z 197 28 located locate VBN cord-323428-jd91k19z 197 29 at at IN cord-323428-jd91k19z 197 30 position position NN cord-323428-jd91k19z 197 31 437 437 CD cord-323428-jd91k19z 197 32 - - SYM cord-323428-jd91k19z 197 33 445 445 CD cord-323428-jd91k19z 197 34 within within IN cord-323428-jd91k19z 197 35 the the DT cord-323428-jd91k19z 197 36 conserved conserve VBN cord-323428-jd91k19z 197 37 region region NN cord-323428-jd91k19z 197 38 of of IN cord-323428-jd91k19z 197 39 the the DT cord-323428-jd91k19z 197 40 S S NNP cord-323428-jd91k19z 197 41 glycoprotein glycoprotein NN cord-323428-jd91k19z 197 42 among among IN cord-323428-jd91k19z 197 43 all all DT cord-323428-jd91k19z 197 44 identified identify VBN cord-323428-jd91k19z 197 45 MERS MERS NNP cord-323428-jd91k19z 197 46 - - HYPH cord-323428-jd91k19z 197 47 CoV cov NN cord-323428-jd91k19z 197 48 strains strain NNS cord-323428-jd91k19z 197 49 . . . cord-323428-jd91k19z 198 1 This this DT cord-323428-jd91k19z 198 2 was be VBD cord-323428-jd91k19z 198 3 selected select VBN cord-323428-jd91k19z 198 4 in in IN cord-323428-jd91k19z 198 5 a a DT cord-323428-jd91k19z 198 6 computerized computerized JJ cord-323428-jd91k19z 198 7 simulation simulation NN cord-323428-jd91k19z 198 8 that that WDT cord-323428-jd91k19z 198 9 showed show VBD cord-323428-jd91k19z 198 10 that that IN cord-323428-jd91k19z 198 11 this this DT cord-323428-jd91k19z 198 12 sequence sequence NN cord-323428-jd91k19z 198 13 has have VBZ cord-323428-jd91k19z 198 14 the the DT cord-323428-jd91k19z 198 15 highest high JJS cord-323428-jd91k19z 198 16 B b NN cord-323428-jd91k19z 198 17 cell cell NN cord-323428-jd91k19z 198 18 antigenicity antigenicity NN cord-323428-jd91k19z 198 19 plot plot NN cord-323428-jd91k19z 198 20 and and CC cord-323428-jd91k19z 198 21 has have VBZ cord-323428-jd91k19z 198 22 the the DT cord-323428-jd91k19z 198 23 ability ability NN cord-323428-jd91k19z 198 24 to to TO cord-323428-jd91k19z 198 25 form form VB cord-323428-jd91k19z 198 26 the the DT cord-323428-jd91k19z 198 27 greatest great JJS cord-323428-jd91k19z 198 28 number number NN cord-323428-jd91k19z 198 29 of of IN cord-323428-jd91k19z 198 30 interactions interaction NNS cord-323428-jd91k19z 198 31 with with IN cord-323428-jd91k19z 198 32 MHC MHC NNP cord-323428-jd91k19z 198 33 - - HYPH cord-323428-jd91k19z 198 34 Class Class NNP cord-323428-jd91k19z 198 35 I -PRON- PRP cord-323428-jd91k19z 198 36 alleles alleles VBP cord-323428-jd91k19z 198 37 [ [ -LRB- cord-323428-jd91k19z 198 38 33 33 CD cord-323428-jd91k19z 198 39 ] ] -RRB- cord-323428-jd91k19z 198 40 . . . cord-323428-jd91k19z 199 1 Although although IN cord-323428-jd91k19z 199 2 this this DT cord-323428-jd91k19z 199 3 epitope epitope NN cord-323428-jd91k19z 199 4 was be VBD cord-323428-jd91k19z 199 5 not not RB cord-323428-jd91k19z 199 6 able able JJ cord-323428-jd91k19z 199 7 to to TO cord-323428-jd91k19z 199 8 increase increase VB cord-323428-jd91k19z 199 9 IFN IFN NNP cord-323428-jd91k19z 199 10 - - HYPH cord-323428-jd91k19z 199 11 γ γ NN cord-323428-jd91k19z 199 12 production production NN cord-323428-jd91k19z 199 13 level level NN cord-323428-jd91k19z 199 14 after after IN cord-323428-jd91k19z 199 15 in in IN cord-323428-jd91k19z 199 16 vitro vitro FW cord-323428-jd91k19z 199 17 stimulation stimulation NN cord-323428-jd91k19z 199 18 , , , cord-323428-jd91k19z 199 19 it -PRON- PRP cord-323428-jd91k19z 199 20 resulted result VBD cord-323428-jd91k19z 199 21 in in IN cord-323428-jd91k19z 199 22 a a DT cord-323428-jd91k19z 199 23 significant significant JJ cord-323428-jd91k19z 199 24 increase increase NN cord-323428-jd91k19z 199 25 in in IN cord-323428-jd91k19z 199 26 IL-12 IL-12 NNP cord-323428-jd91k19z 199 27 production production NN cord-323428-jd91k19z 199 28 and and CC cord-323428-jd91k19z 199 29 a a DT cord-323428-jd91k19z 199 30 significant significant JJ cord-323428-jd91k19z 199 31 decrease decrease NN cord-323428-jd91k19z 199 32 in in IN cord-323428-jd91k19z 199 33 IL-4 IL-4 NNP cord-323428-jd91k19z 199 34 production production NN cord-323428-jd91k19z 199 35 in in IN cord-323428-jd91k19z 199 36 the the DT cord-323428-jd91k19z 199 37 vaccinated vaccinated JJ cord-323428-jd91k19z 199 38 group group NN cord-323428-jd91k19z 199 39 . . . cord-323428-jd91k19z 200 1 In in IN cord-323428-jd91k19z 200 2 addition addition NN cord-323428-jd91k19z 200 3 , , , cord-323428-jd91k19z 200 4 this this DT cord-323428-jd91k19z 200 5 epitope epitope NN cord-323428-jd91k19z 200 6 was be VBD cord-323428-jd91k19z 200 7 able able JJ cord-323428-jd91k19z 200 8 to to TO cord-323428-jd91k19z 200 9 increase increase VB cord-323428-jd91k19z 200 10 IL-4 IL-4 NNP cord-323428-jd91k19z 200 11 production production NN cord-323428-jd91k19z 200 12 after after IN cord-323428-jd91k19z 200 13 in in IN cord-323428-jd91k19z 200 14 vitro vitro FW cord-323428-jd91k19z 200 15 stimulation stimulation NN cord-323428-jd91k19z 200 16 in in IN cord-323428-jd91k19z 200 17 Ad Ad NNP cord-323428-jd91k19z 200 18 - - HYPH cord-323428-jd91k19z 200 19 CMV CMV NNP cord-323428-jd91k19z 200 20 group group NN cord-323428-jd91k19z 200 21 . . . cord-323428-jd91k19z 201 1 However however RB cord-323428-jd91k19z 201 2 , , , cord-323428-jd91k19z 201 3 these these DT cord-323428-jd91k19z 201 4 findings finding NNS cord-323428-jd91k19z 201 5 were be VBD cord-323428-jd91k19z 201 6 not not RB cord-323428-jd91k19z 201 7 enough enough JJ cord-323428-jd91k19z 201 8 to to TO cord-323428-jd91k19z 201 9 prove prove VB cord-323428-jd91k19z 201 10 the the DT cord-323428-jd91k19z 201 11 claim claim NN cord-323428-jd91k19z 201 12 that that IN cord-323428-jd91k19z 201 13 this this DT cord-323428-jd91k19z 201 14 epitope epitope NN cord-323428-jd91k19z 201 15 maybe maybe RB cord-323428-jd91k19z 201 16 has have VBZ cord-323428-jd91k19z 201 17 a a DT cord-323428-jd91k19z 201 18 crucial crucial JJ cord-323428-jd91k19z 201 19 role role NN cord-323428-jd91k19z 201 20 in in IN cord-323428-jd91k19z 201 21 MERS MERS NNP cord-323428-jd91k19z 201 22 - - HYPH cord-323428-jd91k19z 201 23 CoV CoV NNP cord-323428-jd91k19z 201 24 pathogenesis pathogenesis NN cord-323428-jd91k19z 201 25 . . . cord-323428-jd91k19z 202 1 Hence hence RB cord-323428-jd91k19z 202 2 , , , cord-323428-jd91k19z 202 3 further further JJ cord-323428-jd91k19z 202 4 studies study NNS cord-323428-jd91k19z 202 5 ( ( -LRB- cord-323428-jd91k19z 202 6 such such JJ cord-323428-jd91k19z 202 7 as as IN cord-323428-jd91k19z 202 8 in in IN cord-323428-jd91k19z 202 9 vivo vivo NN cord-323428-jd91k19z 202 10 or or CC cord-323428-jd91k19z 202 11 knockout knockout NN cord-323428-jd91k19z 202 12 experiments experiment NNS cord-323428-jd91k19z 202 13 ) ) -RRB- cord-323428-jd91k19z 202 14 should should MD cord-323428-jd91k19z 202 15 be be VB cord-323428-jd91k19z 202 16 conducted conduct VBN cord-323428-jd91k19z 202 17 to to TO cord-323428-jd91k19z 202 18 address address VB cord-323428-jd91k19z 202 19 the the DT cord-323428-jd91k19z 202 20 role role NN cord-323428-jd91k19z 202 21 of of IN cord-323428-jd91k19z 202 22 this this DT cord-323428-jd91k19z 202 23 epitope epitope NN cord-323428-jd91k19z 202 24 in in IN cord-323428-jd91k19z 202 25 MERS MERS NNP cord-323428-jd91k19z 202 26 - - HYPH cord-323428-jd91k19z 202 27 CoV cov NN cord-323428-jd91k19z 202 28 pathogenesis pathogenesis NN cord-323428-jd91k19z 202 29 . . . cord-323428-jd91k19z 203 1 Overall overall RB cord-323428-jd91k19z 203 2 , , , cord-323428-jd91k19z 203 3 the the DT cord-323428-jd91k19z 203 4 adenoviral adenoviral JJ cord-323428-jd91k19z 203 5 vector vector NN cord-323428-jd91k19z 203 6 encoding encoding NN cord-323428-jd91k19z 204 1 the the DT cord-323428-jd91k19z 204 2 S1 S1 NNP cord-323428-jd91k19z 204 3 subunit subunit NN cord-323428-jd91k19z 204 4 of of IN cord-323428-jd91k19z 204 5 the the DT cord-323428-jd91k19z 204 6 MERS MERS NNP cord-323428-jd91k19z 204 7 - - HYPH cord-323428-jd91k19z 204 8 CoV CoV NNP cord-323428-jd91k19z 204 9 genome genome NN cord-323428-jd91k19z 204 10 is be VBZ cord-323428-jd91k19z 204 11 a a DT cord-323428-jd91k19z 204 12 promising promising JJ cord-323428-jd91k19z 204 13 vaccine vaccine NN cord-323428-jd91k19z 204 14 candidate candidate NN cord-323428-jd91k19z 204 15 against against IN cord-323428-jd91k19z 204 16 MERS MERS NNP cord-323428-jd91k19z 204 17 - - HYPH cord-323428-jd91k19z 204 18 CoV CoV NNP cord-323428-jd91k19z 204 19 infection infection NN cord-323428-jd91k19z 204 20 . . . cord-323428-jd91k19z 205 1 It -PRON- PRP cord-323428-jd91k19z 205 2 was be VBD cord-323428-jd91k19z 205 3 clearly clearly RB cord-323428-jd91k19z 205 4 demonstrated demonstrate VBN cord-323428-jd91k19z 205 5 that that IN cord-323428-jd91k19z 205 6 this this DT cord-323428-jd91k19z 205 7 recombinant recombinant JJ cord-323428-jd91k19z 205 8 vaccine vaccine NN cord-323428-jd91k19z 205 9 is be VBZ cord-323428-jd91k19z 205 10 distributed distribute VBN cord-323428-jd91k19z 205 11 in in IN cord-323428-jd91k19z 205 12 host host NN cord-323428-jd91k19z 205 13 tissues tissue NNS cord-323428-jd91k19z 205 14 . . . cord-323428-jd91k19z 206 1 In in IN cord-323428-jd91k19z 206 2 addition addition NN cord-323428-jd91k19z 206 3 , , , cord-323428-jd91k19z 206 4 this this DT cord-323428-jd91k19z 206 5 construct construct NN cord-323428-jd91k19z 206 6 could could MD cord-323428-jd91k19z 206 7 induce induce VB cord-323428-jd91k19z 206 8 production production NN cord-323428-jd91k19z 206 9 of of IN cord-323428-jd91k19z 206 10 a a DT cord-323428-jd91k19z 206 11 specific specific JJ cord-323428-jd91k19z 206 12 anti anti JJ cord-323428-jd91k19z 206 13 - - JJ cord-323428-jd91k19z 206 14 S1 s1 JJ cord-323428-jd91k19z 206 15 IgG IgG NNP cord-323428-jd91k19z 206 16 antibody antibody NN cord-323428-jd91k19z 206 17 response response NN cord-323428-jd91k19z 206 18 as as RB cord-323428-jd91k19z 206 19 well well RB cord-323428-jd91k19z 206 20 as as IN cord-323428-jd91k19z 206 21 the the DT cord-323428-jd91k19z 206 22 Th1 th1 CD cord-323428-jd91k19z 206 23 immune immune JJ cord-323428-jd91k19z 206 24 response response NN cord-323428-jd91k19z 206 25 , , , cord-323428-jd91k19z 206 26 which which WDT cord-323428-jd91k19z 206 27 was be VBD cord-323428-jd91k19z 206 28 evident evident JJ cord-323428-jd91k19z 206 29 from from IN cord-323428-jd91k19z 206 30 the the DT cord-323428-jd91k19z 206 31 increased increase VBN cord-323428-jd91k19z 206 32 levels level NNS cord-323428-jd91k19z 206 33 of of IN cord-323428-jd91k19z 206 34 pro pro JJ cord-323428-jd91k19z 206 35 - - JJ cord-323428-jd91k19z 206 36 inflammatory inflammatory JJ cord-323428-jd91k19z 206 37 cytokines cytokine NNS cord-323428-jd91k19z 206 38 in in IN cord-323428-jd91k19z 206 39 the the DT cord-323428-jd91k19z 206 40 vaccinated vaccinate VBN cord-323428-jd91k19z 206 41 animals animal NNS cord-323428-jd91k19z 206 42 . . . cord-323428-jd91k19z 207 1 Isolation isolation NN cord-323428-jd91k19z 207 2 of of IN cord-323428-jd91k19z 207 3 a a DT cord-323428-jd91k19z 207 4 novel novel JJ cord-323428-jd91k19z 207 5 coronavirus coronavirus NN cord-323428-jd91k19z 207 6 from from IN cord-323428-jd91k19z 207 7 a a DT cord-323428-jd91k19z 207 8 man man NN cord-323428-jd91k19z 207 9 with with IN cord-323428-jd91k19z 207 10 pneumonia pneumonia NN cord-323428-jd91k19z 207 11 in in IN cord-323428-jd91k19z 207 12 Saudi Saudi NNP cord-323428-jd91k19z 207 13 Arabia Arabia NNP cord-323428-jd91k19z 207 14 Middle Middle NNP cord-323428-jd91k19z 207 15 East East NNP cord-323428-jd91k19z 207 16 respiratory respiratory JJ cord-323428-jd91k19z 207 17 syndrome syndrome NN cord-323428-jd91k19z 207 18 coronavirus coronavirus NN cord-323428-jd91k19z 207 19 : : : cord-323428-jd91k19z 207 20 Transmission transmission NN cord-323428-jd91k19z 207 21 and and CC cord-323428-jd91k19z 207 22 phylogenetic phylogenetic JJ cord-323428-jd91k19z 207 23 evolution evolution NN cord-323428-jd91k19z 207 24 MERS mers RB cord-323428-jd91k19z 207 25 : : : cord-323428-jd91k19z 208 1 Emergence emergence NN cord-323428-jd91k19z 208 2 of of IN cord-323428-jd91k19z 208 3 a a DT cord-323428-jd91k19z 208 4 novel novel JJ cord-323428-jd91k19z 208 5 human human JJ cord-323428-jd91k19z 208 6 coronavirus coronavirus NN cord-323428-jd91k19z 208 7 Middle Middle NNP cord-323428-jd91k19z 208 8 East East NNP cord-323428-jd91k19z 208 9 respiratory respiratory JJ cord-323428-jd91k19z 208 10 syndrome syndrome NN cord-323428-jd91k19z 208 11 coronavirus coronavirus NN cord-323428-jd91k19z 208 12 " " '' cord-323428-jd91k19z 208 13 MERS MERS NNP cord-323428-jd91k19z 208 14 - - HYPH cord-323428-jd91k19z 208 15 CoV CoV NNP cord-323428-jd91k19z 208 16 " " '' cord-323428-jd91k19z 208 17 : : : cord-323428-jd91k19z 208 18 Current current JJ cord-323428-jd91k19z 208 19 knowledge knowledge NN cord-323428-jd91k19z 208 20 gaps gap NNS cord-323428-jd91k19z 208 21 Genomic genomic JJ cord-323428-jd91k19z 208 22 characterization characterization NN cord-323428-jd91k19z 208 23 of of IN cord-323428-jd91k19z 208 24 a a DT cord-323428-jd91k19z 208 25 newly newly RB cord-323428-jd91k19z 208 26 discovered discover VBN cord-323428-jd91k19z 208 27 coronavirus coronavirus NN cord-323428-jd91k19z 208 28 associated associate VBN cord-323428-jd91k19z 208 29 with with IN cord-323428-jd91k19z 208 30 acute acute JJ cord-323428-jd91k19z 208 31 respiratory respiratory JJ cord-323428-jd91k19z 208 32 distress distress NN cord-323428-jd91k19z 208 33 syndrome syndrome NN cord-323428-jd91k19z 208 34 in in IN cord-323428-jd91k19z 208 35 humans human NNS cord-323428-jd91k19z 208 36 MERS MERS NNP cord-323428-jd91k19z 208 37 - - HYPH cord-323428-jd91k19z 208 38 CoV CoV NNP cord-323428-jd91k19z 208 39 -- -- : cord-323428-jd91k19z 208 40 are be VBP cord-323428-jd91k19z 208 41 we -PRON- PRP cord-323428-jd91k19z 208 42 on on IN cord-323428-jd91k19z 208 43 the the DT cord-323428-jd91k19z 208 44 verge verge NN cord-323428-jd91k19z 208 45 of of IN cord-323428-jd91k19z 208 46 a a DT cord-323428-jd91k19z 208 47 pandemic pandemic NN cord-323428-jd91k19z 208 48 ? ? . cord-323428-jd91k19z 209 1 Middle Middle NNP cord-323428-jd91k19z 209 2 East East NNP cord-323428-jd91k19z 209 3 respiratory respiratory JJ cord-323428-jd91k19z 209 4 syndrome syndrome NN cord-323428-jd91k19z 209 5 coronavirus coronavirus NN cord-323428-jd91k19z 209 6 ( ( -LRB- cord-323428-jd91k19z 209 7 MERS MERS NNP cord-323428-jd91k19z 209 8 - - HYPH cord-323428-jd91k19z 209 9 CoV CoV NNP cord-323428-jd91k19z 209 10 ) ) -RRB- cord-323428-jd91k19z 209 11 serology serology NN cord-323428-jd91k19z 209 12 in in IN cord-323428-jd91k19z 209 13 major major JJ cord-323428-jd91k19z 209 14 livestock livestock NN cord-323428-jd91k19z 209 15 species specie NNS cord-323428-jd91k19z 209 16 in in IN cord-323428-jd91k19z 209 17 an an DT cord-323428-jd91k19z 209 18 affected affected JJ cord-323428-jd91k19z 209 19 region region NN cord-323428-jd91k19z 209 20 in in IN cord-323428-jd91k19z 209 21 Jordan Jordan NNP cord-323428-jd91k19z 210 1 Middle Middle NNP cord-323428-jd91k19z 210 2 East East NNP cord-323428-jd91k19z 210 3 respiratory respiratory JJ cord-323428-jd91k19z 210 4 syndrome syndrome NN cord-323428-jd91k19z 210 5 ( ( -LRB- cord-323428-jd91k19z 210 6 MERS MERS NNP cord-323428-jd91k19z 210 7 ) ) -RRB- cord-323428-jd91k19z 211 1 coronavirus coronavirus NN cord-323428-jd91k19z 211 2 seroprevalence seroprevalence NN cord-323428-jd91k19z 211 3 in in IN cord-323428-jd91k19z 211 4 domestic domestic JJ cord-323428-jd91k19z 211 5 livestock livestock NN cord-323428-jd91k19z 211 6 in in IN cord-323428-jd91k19z 211 7 Saudi Saudi NNP cord-323428-jd91k19z 211 8 Arabia Arabia NNP cord-323428-jd91k19z 211 9 Middle Middle NNP cord-323428-jd91k19z 211 10 East East NNP cord-323428-jd91k19z 211 11 respiratory respiratory JJ cord-323428-jd91k19z 211 12 syndrome syndrome NN cord-323428-jd91k19z 211 13 coronavirus coronavirus NN cord-323428-jd91k19z 211 14 in in IN cord-323428-jd91k19z 211 15 dromedary dromedary NN cord-323428-jd91k19z 211 16 camels camel NNS cord-323428-jd91k19z 211 17 : : : cord-323428-jd91k19z 212 1 An an DT cord-323428-jd91k19z 212 2 outbreak outbreak NN cord-323428-jd91k19z 212 3 investigation investigation NN cord-323428-jd91k19z 212 4 Antibodies Antibodies NNP cord-323428-jd91k19z 212 5 against against IN cord-323428-jd91k19z 212 6 MERS MERS NNP cord-323428-jd91k19z 212 7 coronavirus coronavirus NN cord-323428-jd91k19z 212 8 in in IN cord-323428-jd91k19z 212 9 dromedary dromedary NN cord-323428-jd91k19z 212 10 camels camel NNS cord-323428-jd91k19z 212 11 Detection Detection NNP cord-323428-jd91k19z 212 12 of of IN cord-323428-jd91k19z 212 13 the the DT cord-323428-jd91k19z 212 14 Middle Middle NNP cord-323428-jd91k19z 212 15 East East NNP cord-323428-jd91k19z 212 16 respiratory respiratory JJ cord-323428-jd91k19z 212 17 syndrome syndrome NN cord-323428-jd91k19z 212 18 coronavirus coronavirus NN cord-323428-jd91k19z 212 19 genome genome NN cord-323428-jd91k19z 212 20 in in IN cord-323428-jd91k19z 212 21 an an DT cord-323428-jd91k19z 212 22 air air NN cord-323428-jd91k19z 212 23 sample sample NN cord-323428-jd91k19z 212 24 originating originate VBG cord-323428-jd91k19z 212 25 from from IN cord-323428-jd91k19z 212 26 a a DT cord-323428-jd91k19z 212 27 camel camel NN cord-323428-jd91k19z 212 28 barn barn NN cord-323428-jd91k19z 212 29 owned own VBN cord-323428-jd91k19z 212 30 by by IN cord-323428-jd91k19z 212 31 an an DT cord-323428-jd91k19z 212 32 infected infected JJ cord-323428-jd91k19z 212 33 patient patient NN cord-323428-jd91k19z 212 34 Severe severe JJ cord-323428-jd91k19z 212 35 acute acute JJ cord-323428-jd91k19z 212 36 respiratory respiratory JJ cord-323428-jd91k19z 212 37 syndrome syndrome NN cord-323428-jd91k19z 212 38 vaccine vaccine NN cord-323428-jd91k19z 212 39 efficacy efficacy NN cord-323428-jd91k19z 212 40 in in IN cord-323428-jd91k19z 212 41 ferrets ferret NNS cord-323428-jd91k19z 212 42 : : : cord-323428-jd91k19z 213 1 whole whole RB cord-323428-jd91k19z 213 2 killed kill VBN cord-323428-jd91k19z 213 3 virus virus NN cord-323428-jd91k19z 213 4 and and CC cord-323428-jd91k19z 213 5 adenovirus adenovirus NN cord-323428-jd91k19z 213 6 - - HYPH cord-323428-jd91k19z 213 7 vectored vectore VBN cord-323428-jd91k19z 213 8 vaccines vaccine NNS cord-323428-jd91k19z 213 9 Systemic systemic JJ cord-323428-jd91k19z 213 10 and and CC cord-323428-jd91k19z 213 11 mucosal mucosal JJ cord-323428-jd91k19z 213 12 immunity immunity NN cord-323428-jd91k19z 213 13 in in IN cord-323428-jd91k19z 213 14 mice mouse NNS cord-323428-jd91k19z 213 15 elicited elicit VBN cord-323428-jd91k19z 213 16 by by IN cord-323428-jd91k19z 213 17 a a DT cord-323428-jd91k19z 213 18 single single JJ cord-323428-jd91k19z 213 19 immunization immunization NN cord-323428-jd91k19z 213 20 with with IN cord-323428-jd91k19z 213 21 human human JJ cord-323428-jd91k19z 213 22 adenovirus adenovirus NN cord-323428-jd91k19z 213 23 Type type NN cord-323428-jd91k19z 213 24 5 5 CD cord-323428-jd91k19z 213 25 or or CC cord-323428-jd91k19z 213 26 41 41 CD cord-323428-jd91k19z 213 27 vector vector NN cord-323428-jd91k19z 213 28 - - HYPH cord-323428-jd91k19z 213 29 based base VBN cord-323428-jd91k19z 213 30 vaccines vaccine NNS cord-323428-jd91k19z 213 31 carrying carry VBG cord-323428-jd91k19z 213 32 the the DT cord-323428-jd91k19z 213 33 spike spike NN cord-323428-jd91k19z 213 34 protein protein NN cord-323428-jd91k19z 213 35 of of IN cord-323428-jd91k19z 213 36 Middle Middle NNP cord-323428-jd91k19z 213 37 East East NNP cord-323428-jd91k19z 213 38 respiratory respiratory JJ cord-323428-jd91k19z 213 39 syndrome syndrome NN cord-323428-jd91k19z 213 40 coronavirus coronavirus NN cord-323428-jd91k19z 213 41 Immunogenicity Immunogenicity NNP cord-323428-jd91k19z 213 42 of of IN cord-323428-jd91k19z 213 43 an an DT cord-323428-jd91k19z 213 44 adenoviral adenoviral NN cord-323428-jd91k19z 213 45 - - HYPH cord-323428-jd91k19z 213 46 based base VBN cord-323428-jd91k19z 213 47 Middle Middle NNP cord-323428-jd91k19z 213 48 East East NNP cord-323428-jd91k19z 213 49 respiratory respiratory JJ cord-323428-jd91k19z 213 50 syndrome syndrome NN cord-323428-jd91k19z 213 51 coronavirus coronavirus NN cord-323428-jd91k19z 213 52 vaccine vaccine NN cord-323428-jd91k19z 213 53 in in IN cord-323428-jd91k19z 213 54 BALB BALB NNP cord-323428-jd91k19z 213 55 / / SYM cord-323428-jd91k19z 213 56 c c NN cord-323428-jd91k19z 213 57 mice mouse NNS cord-323428-jd91k19z 213 58 Middle Middle NNP cord-323428-jd91k19z 213 59 East East NNP cord-323428-jd91k19z 214 1 respiratory respiratory JJ cord-323428-jd91k19z 214 2 syndrome syndrome NN cord-323428-jd91k19z 214 3 coronavirus coronavirus NN cord-323428-jd91k19z 214 4 spike spike NN cord-323428-jd91k19z 214 5 protein protein NN cord-323428-jd91k19z 214 6 delivered deliver VBN cord-323428-jd91k19z 214 7 by by IN cord-323428-jd91k19z 214 8 modified modify VBN cord-323428-jd91k19z 214 9 vaccinia vaccinia NN cord-323428-jd91k19z 214 10 virus virus NN cord-323428-jd91k19z 214 11 Ankara Ankara NNP cord-323428-jd91k19z 214 12 efficiently efficiently RB cord-323428-jd91k19z 214 13 induces induce VBZ cord-323428-jd91k19z 214 14 virus virus NN cord-323428-jd91k19z 214 15 - - HYPH cord-323428-jd91k19z 214 16 neutralizing neutralize VBG cord-323428-jd91k19z 214 17 antibodies antibody NNS cord-323428-jd91k19z 214 18 Protective protective JJ cord-323428-jd91k19z 214 19 efficacy efficacy NN cord-323428-jd91k19z 214 20 of of IN cord-323428-jd91k19z 214 21 recombinant recombinant JJ cord-323428-jd91k19z 214 22 modified modify VBN cord-323428-jd91k19z 214 23 vaccinia vaccinia NN cord-323428-jd91k19z 214 24 virus virus NN cord-323428-jd91k19z 214 25 ankara ankara NNP cord-323428-jd91k19z 214 26 delivering deliver VBG cord-323428-jd91k19z 214 27 Middle Middle NNP cord-323428-jd91k19z 214 28 East East NNP cord-323428-jd91k19z 214 29 respiratory respiratory JJ cord-323428-jd91k19z 214 30 syndrome syndrome NN cord-323428-jd91k19z 214 31 coronavirus coronavirus NN cord-323428-jd91k19z 214 32 spike spike NN cord-323428-jd91k19z 214 33 glycoprotein glycoprotein NN cord-323428-jd91k19z 214 34 Heterologous heterologous JJ cord-323428-jd91k19z 214 35 prime prime JJ cord-323428-jd91k19z 214 36 - - HYPH cord-323428-jd91k19z 214 37 boost boost NN cord-323428-jd91k19z 214 38 vaccination vaccination NN cord-323428-jd91k19z 214 39 with with IN cord-323428-jd91k19z 215 1 adenoviral adenoviral NNP cord-323428-jd91k19z 215 2 vector vector NN cord-323428-jd91k19z 215 3 and and CC cord-323428-jd91k19z 215 4 protein protein NN cord-323428-jd91k19z 215 5 nanoparticles nanoparticle NNS cord-323428-jd91k19z 215 6 induces induce VBZ cord-323428-jd91k19z 215 7 both both CC cord-323428-jd91k19z 215 8 Th Th NNP cord-323428-jd91k19z 215 9 1 1 CD cord-323428-jd91k19z 215 10 and and CC cord-323428-jd91k19z 215 11 Th Th NNP cord-323428-jd91k19z 215 12 2 2 CD cord-323428-jd91k19z 215 13 responses response NNS cord-323428-jd91k19z 215 14 against against IN cord-323428-jd91k19z 215 15 Middle Middle NNP cord-323428-jd91k19z 215 16 East East NNP cord-323428-jd91k19z 215 17 respiratory respiratory JJ cord-323428-jd91k19z 215 18 syndrome syndrome NN cord-323428-jd91k19z 215 19 coronavirus coronavirus NN cord-323428-jd91k19z 215 20 Distribution distribution NN cord-323428-jd91k19z 215 21 and and CC cord-323428-jd91k19z 215 22 absence absence NN cord-323428-jd91k19z 215 23 of of IN cord-323428-jd91k19z 215 24 generalized generalized JJ cord-323428-jd91k19z 215 25 lesions lesion NNS cord-323428-jd91k19z 215 26 in in IN cord-323428-jd91k19z 215 27 mice mouse NNS cord-323428-jd91k19z 215 28 following follow VBG cord-323428-jd91k19z 215 29 single single JJ cord-323428-jd91k19z 215 30 - - HYPH cord-323428-jd91k19z 215 31 dose dose NN cord-323428-jd91k19z 215 32 intramuscular intramuscular JJ cord-323428-jd91k19z 215 33 inoculation inoculation NN cord-323428-jd91k19z 215 34 of of IN cord-323428-jd91k19z 215 35 the the DT cord-323428-jd91k19z 215 36 vaccine vaccine NN cord-323428-jd91k19z 215 37 candidate candidate NN cord-323428-jd91k19z 215 38 MVA MVA NNP cord-323428-jd91k19z 215 39 - - HYPH cord-323428-jd91k19z 215 40 MERS MERS NNP cord-323428-jd91k19z 215 41 - - HYPH cord-323428-jd91k19z 215 42 S S NNP cord-323428-jd91k19z 215 43 Immunogenicity Immunogenicity NNP cord-323428-jd91k19z 215 44 of of IN cord-323428-jd91k19z 215 45 different different JJ cord-323428-jd91k19z 215 46 forms form NNS cord-323428-jd91k19z 215 47 of of IN cord-323428-jd91k19z 215 48 Middle Middle NNP cord-323428-jd91k19z 215 49 East East NNP cord-323428-jd91k19z 215 50 respiratory respiratory JJ cord-323428-jd91k19z 215 51 syndrome syndrome NN cord-323428-jd91k19z 215 52 s s NN cord-323428-jd91k19z 215 53 glycoprotein glycoprotein NN cord-323428-jd91k19z 215 54 ChAdOx1 ChAdOx1 NNP cord-323428-jd91k19z 215 55 and and CC cord-323428-jd91k19z 215 56 MVA MVA NNP cord-323428-jd91k19z 215 57 based base VBN cord-323428-jd91k19z 215 58 vaccine vaccine NN cord-323428-jd91k19z 215 59 candidates candidate NNS cord-323428-jd91k19z 215 60 against against IN cord-323428-jd91k19z 215 61 MERS MERS NNP cord-323428-jd91k19z 215 62 - - HYPH cord-323428-jd91k19z 215 63 CoV CoV NNP cord-323428-jd91k19z 215 64 elicit elicit VBP cord-323428-jd91k19z 215 65 neutralising neutralise VBG cord-323428-jd91k19z 215 66 antibodies antibody NNS cord-323428-jd91k19z 215 67 and and CC cord-323428-jd91k19z 215 68 cellular cellular JJ cord-323428-jd91k19z 215 69 immune immune JJ cord-323428-jd91k19z 215 70 responses response NNS cord-323428-jd91k19z 215 71 in in IN cord-323428-jd91k19z 215 72 mice mouse NNS cord-323428-jd91k19z 215 73 Protective protective JJ cord-323428-jd91k19z 215 74 efficacy efficacy NN cord-323428-jd91k19z 215 75 of of IN cord-323428-jd91k19z 215 76 a a DT cord-323428-jd91k19z 215 77 novel novel JJ cord-323428-jd91k19z 215 78 simian simian JJ cord-323428-jd91k19z 215 79 adenovirus adenovirus NN cord-323428-jd91k19z 215 80 vaccine vaccine NN cord-323428-jd91k19z 215 81 against against IN cord-323428-jd91k19z 215 82 lethal lethal JJ cord-323428-jd91k19z 215 83 MERS MERS NNP cord-323428-jd91k19z 215 84 - - HYPH cord-323428-jd91k19z 215 85 CoV cov NN cord-323428-jd91k19z 215 86 challenge challenge NN cord-323428-jd91k19z 215 87 in in IN cord-323428-jd91k19z 215 88 a a DT cord-323428-jd91k19z 215 89 transgenic transgenic JJ cord-323428-jd91k19z 215 90 human human JJ cord-323428-jd91k19z 215 91 DPP4 DPP4 NNP cord-323428-jd91k19z 215 92 mouse mouse NN cord-323428-jd91k19z 215 93 model model NN cord-323428-jd91k19z 215 94 Single single JJ cord-323428-jd91k19z 215 95 intranasal intranasal NN cord-323428-jd91k19z 215 96 immunization immunization NN cord-323428-jd91k19z 215 97 with with IN cord-323428-jd91k19z 215 98 chimpanzee chimpanzee NN cord-323428-jd91k19z 215 99 adenovirus adenovirus NN cord-323428-jd91k19z 215 100 - - HYPH cord-323428-jd91k19z 215 101 based base VBN cord-323428-jd91k19z 215 102 vaccine vaccine NN cord-323428-jd91k19z 215 103 induces induce VBZ cord-323428-jd91k19z 215 104 sustained sustained JJ cord-323428-jd91k19z 215 105 and and CC cord-323428-jd91k19z 215 106 protective protective JJ cord-323428-jd91k19z 215 107 immunity immunity NN cord-323428-jd91k19z 215 108 against against IN cord-323428-jd91k19z 215 109 MERS MERS NNP cord-323428-jd91k19z 215 110 - - HYPH cord-323428-jd91k19z 215 111 CoV CoV NNP cord-323428-jd91k19z 215 112 infection infection NN cord-323428-jd91k19z 216 1 A a DT cord-323428-jd91k19z 216 2 highly highly RB cord-323428-jd91k19z 216 3 immunogenic immunogenic JJ cord-323428-jd91k19z 216 4 , , , cord-323428-jd91k19z 216 5 protective protective JJ cord-323428-jd91k19z 216 6 and and CC cord-323428-jd91k19z 216 7 safe safe JJ cord-323428-jd91k19z 216 8 adenovirus adenovirus NN cord-323428-jd91k19z 216 9 - - HYPH cord-323428-jd91k19z 216 10 based base VBN cord-323428-jd91k19z 216 11 vaccine vaccine NN cord-323428-jd91k19z 216 12 expressing express VBG cord-323428-jd91k19z 216 13 MERS MERS NNP cord-323428-jd91k19z 216 14 - - HYPH cord-323428-jd91k19z 216 15 CoV CoV NNP cord-323428-jd91k19z 216 16 S1-CD40L S1-CD40L NNP cord-323428-jd91k19z 216 17 fusion fusion NN cord-323428-jd91k19z 216 18 protein protein NN cord-323428-jd91k19z 216 19 in in IN cord-323428-jd91k19z 216 20 transgenic transgenic JJ cord-323428-jd91k19z 216 21 human human JJ cord-323428-jd91k19z 216 22 DPP4 DPP4 NNP cord-323428-jd91k19z 216 23 mouse mouse NN cord-323428-jd91k19z 216 24 model model NN cord-323428-jd91k19z 216 25 Dipeptidyl Dipeptidyl NNP cord-323428-jd91k19z 216 26 peptidase peptidase NN cord-323428-jd91k19z 216 27 4 4 CD cord-323428-jd91k19z 216 28 is be VBZ cord-323428-jd91k19z 216 29 a a DT cord-323428-jd91k19z 216 30 functional functional JJ cord-323428-jd91k19z 216 31 receptor receptor NN cord-323428-jd91k19z 216 32 for for IN cord-323428-jd91k19z 216 33 the the DT cord-323428-jd91k19z 216 34 emerging emerge VBG cord-323428-jd91k19z 216 35 human human JJ cord-323428-jd91k19z 216 36 coronavirus coronavirus NN cord-323428-jd91k19z 216 37 - - HYPH cord-323428-jd91k19z 216 38 EMC EMC NNP cord-323428-jd91k19z 217 1 The the DT cord-323428-jd91k19z 217 2 receptor receptor NN cord-323428-jd91k19z 217 3 - - HYPH cord-323428-jd91k19z 217 4 binding bind VBG cord-323428-jd91k19z 217 5 domain domain NN cord-323428-jd91k19z 217 6 of of IN cord-323428-jd91k19z 217 7 MERS MERS NNP cord-323428-jd91k19z 217 8 - - HYPH cord-323428-jd91k19z 217 9 CoV CoV NNP cord-323428-jd91k19z 217 10 : : : cord-323428-jd91k19z 217 11 The the DT cord-323428-jd91k19z 217 12 dawn dawn NN cord-323428-jd91k19z 217 13 of of IN cord-323428-jd91k19z 217 14 vaccine vaccine NN cord-323428-jd91k19z 217 15 and and CC cord-323428-jd91k19z 217 16 treatment treatment NN cord-323428-jd91k19z 217 17 development development NN cord-323428-jd91k19z 217 18 Adenoviral Adenoviral NNP cord-323428-jd91k19z 217 19 expression expression NN cord-323428-jd91k19z 217 20 of of IN cord-323428-jd91k19z 217 21 a a DT cord-323428-jd91k19z 217 22 truncated truncated JJ cord-323428-jd91k19z 217 23 S1 s1 NN cord-323428-jd91k19z 217 24 subunit subunit NN cord-323428-jd91k19z 217 25 of of IN cord-323428-jd91k19z 217 26 SARS SARS NNP cord-323428-jd91k19z 217 27 - - HYPH cord-323428-jd91k19z 217 28 CoV CoV NNP cord-323428-jd91k19z 217 29 spike spike NN cord-323428-jd91k19z 217 30 protein protein NN cord-323428-jd91k19z 217 31 results result NNS cord-323428-jd91k19z 217 32 in in IN cord-323428-jd91k19z 217 33 specific specific JJ cord-323428-jd91k19z 217 34 humoral humoral JJ cord-323428-jd91k19z 217 35 immune immune JJ cord-323428-jd91k19z 217 36 responses response NNS cord-323428-jd91k19z 217 37 against against IN cord-323428-jd91k19z 217 38 SARS SARS NNP cord-323428-jd91k19z 217 39 - - HYPH cord-323428-jd91k19z 217 40 CoV CoV NNP cord-323428-jd91k19z 217 41 in in IN cord-323428-jd91k19z 217 42 rats rat NNS cord-323428-jd91k19z 217 43 Available available JJ cord-323428-jd91k19z 217 44 at at IN cord-323428-jd91k19z 217 45 www.veterinaryworld.org www.veterinaryworld.org NN cord-323428-jd91k19z 218 1 Comparative comparative JJ cord-323428-jd91k19z 218 2 evaluation evaluation NN cord-323428-jd91k19z 218 3 of of IN cord-323428-jd91k19z 218 4 two two CD cord-323428-jd91k19z 218 5 severe severe JJ cord-323428-jd91k19z 218 6 acute acute JJ cord-323428-jd91k19z 218 7 respiratory respiratory JJ cord-323428-jd91k19z 218 8 syndrome syndrome NN cord-323428-jd91k19z 218 9 ( ( -LRB- cord-323428-jd91k19z 219 1 SARS SARS NNP cord-323428-jd91k19z 219 2 ) ) -RRB- cord-323428-jd91k19z 219 3 vaccine vaccine NN cord-323428-jd91k19z 219 4 candidates candidate NNS cord-323428-jd91k19z 219 5 in in IN cord-323428-jd91k19z 219 6 mice mouse NNS cord-323428-jd91k19z 219 7 challenged challenge VBN cord-323428-jd91k19z 219 8 with with IN cord-323428-jd91k19z 219 9 SARS SARS NNP cord-323428-jd91k19z 219 10 coronavirus coronavirus NN cord-323428-jd91k19z 220 1 The the DT cord-323428-jd91k19z 220 2 Gene Gene NNP cord-323428-jd91k19z 220 3 Delivery Delivery NNP cord-323428-jd91k19z 220 4 Company Company NNP cord-323428-jd91k19z 220 5 ) ) -RRB- cord-323428-jd91k19z 221 1 : : : cord-323428-jd91k19z 221 2 Recombinant Recombinant NNP cord-323428-jd91k19z 221 3 Adenovirus Adenovirus NNP cord-323428-jd91k19z 221 4 Construction Construction NNP cord-323428-jd91k19z 221 5 Middle Middle NNP cord-323428-jd91k19z 221 6 East East NNP cord-323428-jd91k19z 221 7 respiratory respiratory JJ cord-323428-jd91k19z 221 8 syndrome syndrome NN cord-323428-jd91k19z 221 9 coronavirus coronavirus NN cord-323428-jd91k19z 221 10 infection infection NN cord-323428-jd91k19z 221 11 in in IN cord-323428-jd91k19z 221 12 dromedary dromedary NN cord-323428-jd91k19z 221 13 camels camel NNS cord-323428-jd91k19z 221 14 in in IN cord-323428-jd91k19z 221 15 Saudi Saudi NNP cord-323428-jd91k19z 221 16 Arabia Arabia NNP cord-323428-jd91k19z 221 17 Endogenous endogenous JJ cord-323428-jd91k19z 221 18 biotin biotin NN cord-323428-jd91k19z 221 19 expression expression NN cord-323428-jd91k19z 221 20 in in IN cord-323428-jd91k19z 221 21 renal renal JJ cord-323428-jd91k19z 221 22 and and CC cord-323428-jd91k19z 221 23 testicular testicular JJ cord-323428-jd91k19z 221 24 tumors tumor NNS cord-323428-jd91k19z 221 25 and and CC cord-323428-jd91k19z 221 26 literature literature NN cord-323428-jd91k19z 221 27 review review NN cord-323428-jd91k19z 221 28 Retrieved retrieve VBD cord-323428-jd91k19z 221 29 endogenous endogenous JJ cord-323428-jd91k19z 221 30 biotin biotin NN cord-323428-jd91k19z 221 31 : : : cord-323428-jd91k19z 222 1 A a DT cord-323428-jd91k19z 222 2 novel novel JJ cord-323428-jd91k19z 222 3 marker marker NN cord-323428-jd91k19z 222 4 and and CC cord-323428-jd91k19z 222 5 a a DT cord-323428-jd91k19z 222 6 potential potential JJ cord-323428-jd91k19z 222 7 pitfall pitfall NN cord-323428-jd91k19z 222 8 in in IN cord-323428-jd91k19z 222 9 diagnostic diagnostic JJ cord-323428-jd91k19z 222 10 immunohistochemistry immunohistochemistry NN cord-323428-jd91k19z 222 11 Effects effect NNS cord-323428-jd91k19z 222 12 of of IN cord-323428-jd91k19z 222 13 gamma gamma NN cord-323428-jd91k19z 222 14 interferon interferon NN cord-323428-jd91k19z 222 15 , , , cord-323428-jd91k19z 222 16 interleukin-10 interleukin-10 NNP cord-323428-jd91k19z 222 17 , , , cord-323428-jd91k19z 222 18 and and CC cord-323428-jd91k19z 222 19 transforming transform VBG cord-323428-jd91k19z 222 20 growth growth NN cord-323428-jd91k19z 222 21 factor factor NN cord-323428-jd91k19z 222 22 beta beta NN cord-323428-jd91k19z 222 23 on on IN cord-323428-jd91k19z 222 24 the the DT cord-323428-jd91k19z 222 25 survival survival NN cord-323428-jd91k19z 222 26 of of IN cord-323428-jd91k19z 222 27 Mycobacterium Mycobacterium NNP cord-323428-jd91k19z 222 28 avium avium NN cord-323428-jd91k19z 222 29 subsp subsp NN cord-323428-jd91k19z 222 30 . . . cord-323428-jd91k19z 223 1 paratuberculosis paratuberculosis NNP cord-323428-jd91k19z 223 2 in in IN cord-323428-jd91k19z 223 3 monocyte monocyte NNP cord-323428-jd91k19z 223 4 - - HYPH cord-323428-jd91k19z 223 5 derived derive VBN cord-323428-jd91k19z 223 6 macrophages macrophage NNS cord-323428-jd91k19z 223 7 from from IN cord-323428-jd91k19z 223 8 naturally naturally RB cord-323428-jd91k19z 223 9 infected infect VBN cord-323428-jd91k19z 223 10 cattle cattle NNS cord-323428-jd91k19z 223 11 Computer computer NN cord-323428-jd91k19z 223 12 - - HYPH cord-323428-jd91k19z 223 13 aided aid VBN cord-323428-jd91k19z 223 14 prediction prediction NN cord-323428-jd91k19z 223 15 and and CC cord-323428-jd91k19z 223 16 identification identification NN cord-323428-jd91k19z 223 17 of of IN cord-323428-jd91k19z 223 18 potential potential JJ cord-323428-jd91k19z 223 19 epitopes epitope NNS cord-323428-jd91k19z 223 20 in in IN cord-323428-jd91k19z 223 21 the the DT cord-323428-jd91k19z 223 22 receptor receptor NN cord-323428-jd91k19z 223 23 - - HYPH cord-323428-jd91k19z 223 24 binding bind VBG cord-323428-jd91k19z 223 25 domain domain NN cord-323428-jd91k19z 223 26 ( ( -LRB- cord-323428-jd91k19z 223 27 RBD RBD NNP cord-323428-jd91k19z 223 28 ) ) -RRB- cord-323428-jd91k19z 223 29 of of IN cord-323428-jd91k19z 223 30 spike spike NN cord-323428-jd91k19z 223 31 ( ( -LRB- cord-323428-jd91k19z 223 32 S s NN cord-323428-jd91k19z 223 33 ) ) -RRB- cord-323428-jd91k19z 223 34 glycoprotein glycoprotein NN cord-323428-jd91k19z 223 35 of of IN cord-323428-jd91k19z 223 36 MERS MERS NNP cord-323428-jd91k19z 223 37 - - HYPH cord-323428-jd91k19z 223 38 CoV CoV NNP cord-323428-jd91k19z 223 39 Analysis Analysis NNP cord-323428-jd91k19z 223 40 of of IN cord-323428-jd91k19z 223 41 relative relative JJ cord-323428-jd91k19z 223 42 gene gene NN cord-323428-jd91k19z 223 43 expression expression NN cord-323428-jd91k19z 223 44 data datum NNS cord-323428-jd91k19z 223 45 using use VBG cord-323428-jd91k19z 223 46 real real JJ cord-323428-jd91k19z 223 47 - - HYPH cord-323428-jd91k19z 223 48 time time NN cord-323428-jd91k19z 223 49 quantitative quantitative JJ cord-323428-jd91k19z 223 50 PCR PCR NNP cord-323428-jd91k19z 223 51 and and CC cord-323428-jd91k19z 223 52 the the DT cord-323428-jd91k19z 223 53 2(-Delta 2(-delta CD cord-323428-jd91k19z 223 54 Delta Delta NNP cord-323428-jd91k19z 223 55 C(T C(T NNP cord-323428-jd91k19z 223 56 ) ) -RRB- cord-323428-jd91k19z 223 57 ) ) -RRB- cord-323428-jd91k19z 224 1 method method NN cord-323428-jd91k19z 224 2 Comparison comparison NN cord-323428-jd91k19z 224 3 of of IN cord-323428-jd91k19z 224 4 intranasal intranasal NN cord-323428-jd91k19z 224 5 and and CC cord-323428-jd91k19z 224 6 transcutaneous transcutaneous JJ cord-323428-jd91k19z 224 7 immunization immunization NN cord-323428-jd91k19z 224 8 for for IN cord-323428-jd91k19z 224 9 induction induction NN cord-323428-jd91k19z 224 10 of of IN cord-323428-jd91k19z 224 11 protective protective JJ cord-323428-jd91k19z 224 12 immunity immunity NN cord-323428-jd91k19z 224 13 against against IN cord-323428-jd91k19z 224 14 Chlamydia Chlamydia NNP cord-323428-jd91k19z 224 15 muridarum muridarum NN cord-323428-jd91k19z 224 16 respiratory respiratory JJ cord-323428-jd91k19z 224 17 tract tract NN cord-323428-jd91k19z 224 18 infection infection NN cord-323428-jd91k19z 224 19 Structure Structure NNP cord-323428-jd91k19z 224 20 of of IN cord-323428-jd91k19z 224 21 MERS MERS NNP cord-323428-jd91k19z 224 22 - - HYPH cord-323428-jd91k19z 224 23 CoV CoV NNP cord-323428-jd91k19z 224 24 spike spike NN cord-323428-jd91k19z 224 25 receptor receptor NN cord-323428-jd91k19z 224 26 - - HYPH cord-323428-jd91k19z 224 27 binding bind VBG cord-323428-jd91k19z 224 28 domain domain NN cord-323428-jd91k19z 224 29 complexed complexe VBN cord-323428-jd91k19z 224 30 with with IN cord-323428-jd91k19z 224 31 human human JJ cord-323428-jd91k19z 224 32 receptor receptor NN cord-323428-jd91k19z 225 1 DPP4 DPP4 NNP cord-323428-jd91k19z 225 2 A a DT cord-323428-jd91k19z 225 3 truncated truncated JJ cord-323428-jd91k19z 225 4 receptor receptor NN cord-323428-jd91k19z 225 5 - - HYPH cord-323428-jd91k19z 225 6 binding bind VBG cord-323428-jd91k19z 225 7 domain domain NN cord-323428-jd91k19z 225 8 of of IN cord-323428-jd91k19z 225 9 MERS MERS NNP cord-323428-jd91k19z 225 10 - - HYPH cord-323428-jd91k19z 225 11 CoV CoV NNP cord-323428-jd91k19z 225 12 spike spike NN cord-323428-jd91k19z 225 13 protein protein NN cord-323428-jd91k19z 225 14 potently potently RB cord-323428-jd91k19z 225 15 inhibits inhibit VBZ cord-323428-jd91k19z 225 16 MERS MERS NNP cord-323428-jd91k19z 225 17 - - HYPH cord-323428-jd91k19z 225 18 CoV cov NN cord-323428-jd91k19z 225 19 infection infection NN cord-323428-jd91k19z 225 20 and and CC cord-323428-jd91k19z 225 21 induces induce VBZ cord-323428-jd91k19z 225 22 strong strong JJ cord-323428-jd91k19z 225 23 neutralizing neutralize VBG cord-323428-jd91k19z 225 24 antibody antibody NN cord-323428-jd91k19z 225 25 responses response NNS cord-323428-jd91k19z 225 26 : : : cord-323428-jd91k19z 225 27 Implication implication NN cord-323428-jd91k19z 225 28 for for IN cord-323428-jd91k19z 225 29 developing develop VBG cord-323428-jd91k19z 225 30 therapeutics therapeutic NNS cord-323428-jd91k19z 225 31 and and CC cord-323428-jd91k19z 225 32 vaccines vaccine NNS cord-323428-jd91k19z 225 33 Tropism tropism NN cord-323428-jd91k19z 225 34 of of IN cord-323428-jd91k19z 225 35 and and CC cord-323428-jd91k19z 225 36 innate innate JJ cord-323428-jd91k19z 225 37 immune immune JJ cord-323428-jd91k19z 225 38 responses response NNS cord-323428-jd91k19z 225 39 to to IN cord-323428-jd91k19z 225 40 the the DT cord-323428-jd91k19z 225 41 novel novel JJ cord-323428-jd91k19z 225 42 human human JJ cord-323428-jd91k19z 225 43 betacoronavirus betacoronavirus NN cord-323428-jd91k19z 225 44 lineage lineage NN cord-323428-jd91k19z 225 45 C C NNP cord-323428-jd91k19z 225 46 virus virus NN cord-323428-jd91k19z 225 47 in in IN cord-323428-jd91k19z 225 48 human human JJ cord-323428-jd91k19z 225 49 ex ex FW cord-323428-jd91k19z 225 50 vivo vivo NN cord-323428-jd91k19z 225 51 respiratory respiratory JJ cord-323428-jd91k19z 225 52 organ organ NN cord-323428-jd91k19z 225 53 cultures culture NNS cord-323428-jd91k19z 226 1 In in IN cord-323428-jd91k19z 226 2 - - HYPH cord-323428-jd91k19z 226 3 vitro vitro FW cord-323428-jd91k19z 226 4 renal renal JJ cord-323428-jd91k19z 226 5 epithelial epithelial JJ cord-323428-jd91k19z 226 6 cell cell NN cord-323428-jd91k19z 226 7 infection infection NN cord-323428-jd91k19z 226 8 reveals reveal VBZ cord-323428-jd91k19z 226 9 a a DT cord-323428-jd91k19z 226 10 viral viral JJ cord-323428-jd91k19z 226 11 kidney kidney NN cord-323428-jd91k19z 226 12 tropism tropism NN cord-323428-jd91k19z 226 13 as as IN cord-323428-jd91k19z 226 14 a a DT cord-323428-jd91k19z 226 15 potential potential JJ cord-323428-jd91k19z 226 16 mechanism mechanism NN cord-323428-jd91k19z 226 17 for for IN cord-323428-jd91k19z 226 18 acute acute JJ cord-323428-jd91k19z 226 19 renal renal JJ cord-323428-jd91k19z 226 20 failure failure NN cord-323428-jd91k19z 226 21 during during IN cord-323428-jd91k19z 226 22 Middle Middle NNP cord-323428-jd91k19z 226 23 East East NNP cord-323428-jd91k19z 226 24 respiratory respiratory JJ cord-323428-jd91k19z 226 25 syndrome syndrome NN cord-323428-jd91k19z 226 26 ( ( -LRB- cord-323428-jd91k19z 226 27 MERS MERS NNP cord-323428-jd91k19z 226 28 ) ) -RRB- cord-323428-jd91k19z 227 1 coronavirus coronavirus NN cord-323428-jd91k19z 227 2 infection infection NN cord-323428-jd91k19z 227 3 Dipeptidyl Dipeptidyl NNP cord-323428-jd91k19z 227 4 - - HYPH cord-323428-jd91k19z 227 5 peptidase peptidase NN cord-323428-jd91k19z 227 6 IV IV NNP cord-323428-jd91k19z 227 7 from from IN cord-323428-jd91k19z 227 8 bench bench NN cord-323428-jd91k19z 227 9 to to IN cord-323428-jd91k19z 227 10 bedside bedside NN cord-323428-jd91k19z 227 11 : : : cord-323428-jd91k19z 228 1 An an DT cord-323428-jd91k19z 228 2 update update NN cord-323428-jd91k19z 228 3 on on IN cord-323428-jd91k19z 228 4 structural structural JJ cord-323428-jd91k19z 228 5 properties property NNS cord-323428-jd91k19z 228 6 , , , cord-323428-jd91k19z 228 7 functions function NNS cord-323428-jd91k19z 228 8 , , , cord-323428-jd91k19z 228 9 and and CC cord-323428-jd91k19z 228 10 clinical clinical JJ cord-323428-jd91k19z 228 11 aspects aspect NNS cord-323428-jd91k19z 228 12 of of IN cord-323428-jd91k19z 228 13 the the DT cord-323428-jd91k19z 228 14 enzyme enzyme NN cord-323428-jd91k19z 228 15 DPP DPP NNP cord-323428-jd91k19z 228 16 IV IV NNP cord-323428-jd91k19z 229 1 Th th DT cord-323428-jd91k19z 229 2 1 1 CD cord-323428-jd91k19z 229 3 /Th /th JJ cord-323428-jd91k19z 229 4 2 2 CD cord-323428-jd91k19z 229 5 balance balance NN cord-323428-jd91k19z 229 6 : : : cord-323428-jd91k19z 229 7 The the DT cord-323428-jd91k19z 229 8 hypothesis hypothesis NN cord-323428-jd91k19z 229 9 , , , cord-323428-jd91k19z 229 10 its -PRON- PRP$ cord-323428-jd91k19z 229 11 limitations limitation NNS cord-323428-jd91k19z 229 12 , , , cord-323428-jd91k19z 229 13 and and CC cord-323428-jd91k19z 229 14 implications implication NNS cord-323428-jd91k19z 229 15 for for IN cord-323428-jd91k19z 229 16 health health NN cord-323428-jd91k19z 229 17 and and CC cord-323428-jd91k19z 229 18 disease disease NN cord-323428-jd91k19z 229 19 Adenoviruses adenovirus NNS cord-323428-jd91k19z 229 20 as as IN cord-323428-jd91k19z 229 21 vaccine vaccine NN cord-323428-jd91k19z 229 22 vectors vector NNS cord-323428-jd91k19z 229 23 Replication replication NN cord-323428-jd91k19z 229 24 - - HYPH cord-323428-jd91k19z 229 25 defective defective JJ cord-323428-jd91k19z 229 26 adenovirus adenovirus NN cord-323428-jd91k19z 229 27 serotype serotype NN cord-323428-jd91k19z 229 28 5 5 CD cord-323428-jd91k19z 229 29 vectors vector NNS cord-323428-jd91k19z 229 30 elicit elicit VBP cord-323428-jd91k19z 229 31 durable durable JJ cord-323428-jd91k19z 229 32 cellular cellular JJ cord-323428-jd91k19z 229 33 and and CC cord-323428-jd91k19z 229 34 humoral humoral JJ cord-323428-jd91k19z 229 35 immune immune JJ cord-323428-jd91k19z 229 36 responses response NNS cord-323428-jd91k19z 229 37 in in IN cord-323428-jd91k19z 229 38 nonhuman nonhuman NN cord-323428-jd91k19z 229 39 primates primate NNS cord-323428-jd91k19z 230 1 Intranasal intranasal NN cord-323428-jd91k19z 230 2 vaccination vaccination NN cord-323428-jd91k19z 230 3 with with IN cord-323428-jd91k19z 230 4 recombinant recombinant JJ cord-323428-jd91k19z 230 5 receptor receptor NN cord-323428-jd91k19z 230 6 - - HYPH cord-323428-jd91k19z 230 7 binding bind VBG cord-323428-jd91k19z 230 8 domain domain NN cord-323428-jd91k19z 230 9 of of IN cord-323428-jd91k19z 230 10 MERS MERS NNP cord-323428-jd91k19z 230 11 - - HYPH cord-323428-jd91k19z 230 12 CoV CoV NNP cord-323428-jd91k19z 230 13 spike spike NN cord-323428-jd91k19z 230 14 protein protein NN cord-323428-jd91k19z 230 15 induces induce VBZ cord-323428-jd91k19z 230 16 much much RB cord-323428-jd91k19z 230 17 stronger strong JJR cord-323428-jd91k19z 230 18 local local JJ cord-323428-jd91k19z 230 19 mucosal mucosal JJ cord-323428-jd91k19z 230 20 immune immune JJ cord-323428-jd91k19z 230 21 responses response NNS cord-323428-jd91k19z 230 22 than than IN cord-323428-jd91k19z 230 23 subcutaneous subcutaneous JJ cord-323428-jd91k19z 230 24 immunization immunization NN cord-323428-jd91k19z 230 25 : : : cord-323428-jd91k19z 231 1 Implication implication NN cord-323428-jd91k19z 231 2 for for IN cord-323428-jd91k19z 231 3 designing design VBG cord-323428-jd91k19z 231 4 novel novel JJ cord-323428-jd91k19z 231 5 mucosal mucosal JJ cord-323428-jd91k19z 231 6 MERS MERS NNP cord-323428-jd91k19z 231 7 vaccines vaccine NNS cord-323428-jd91k19z 231 8 Cell Cell NNP cord-323428-jd91k19z 231 9 host host NN cord-323428-jd91k19z 231 10 response response NN cord-323428-jd91k19z 231 11 to to IN cord-323428-jd91k19z 231 12 infection infection NN cord-323428-jd91k19z 231 13 with with IN cord-323428-jd91k19z 231 14 novel novel JJ cord-323428-jd91k19z 231 15 human human JJ cord-323428-jd91k19z 231 16 coronavirus coronavirus NN cord-323428-jd91k19z 231 17 EMC EMC NNP cord-323428-jd91k19z 231 18 predicts predict VBZ cord-323428-jd91k19z 231 19 potential potential JJ cord-323428-jd91k19z 231 20 antivirals antiviral NNS cord-323428-jd91k19z 231 21 and and CC cord-323428-jd91k19z 231 22 important important JJ cord-323428-jd91k19z 231 23 differences difference NNS cord-323428-jd91k19z 231 24 with with IN cord-323428-jd91k19z 231 25 SARS SARS NNP cord-323428-jd91k19z 231 26 coronavirus coronavirus NN cord-323428-jd91k19z 232 1 The the DT cord-323428-jd91k19z 232 2 structural structural JJ cord-323428-jd91k19z 232 3 and and CC cord-323428-jd91k19z 232 4 accessory accessory NN cord-323428-jd91k19z 232 5 proteins protein NNS cord-323428-jd91k19z 232 6 M M NNP cord-323428-jd91k19z 232 7 , , , cord-323428-jd91k19z 232 8 ORF ORF NNP cord-323428-jd91k19z 232 9 4a 4a CD cord-323428-jd91k19z 232 10 , , , cord-323428-jd91k19z 232 11 ORF ORF NNP cord-323428-jd91k19z 232 12 4b 4b NNS cord-323428-jd91k19z 232 13 , , , cord-323428-jd91k19z 232 14 and and CC cord-323428-jd91k19z 232 15 ORF ORF NNP cord-323428-jd91k19z 232 16 5 5 CD cord-323428-jd91k19z 232 17 of of IN cord-323428-jd91k19z 232 18 Middle Middle NNP cord-323428-jd91k19z 232 19 East East NNP cord-323428-jd91k19z 232 20 respiratory respiratory JJ cord-323428-jd91k19z 232 21 syndrome syndrome NN cord-323428-jd91k19z 232 22 coronavirus coronavirus NN cord-323428-jd91k19z 232 23 ( ( -LRB- cord-323428-jd91k19z 232 24 MERS MERS NNP cord-323428-jd91k19z 232 25 - - HYPH cord-323428-jd91k19z 232 26 CoV CoV NNP cord-323428-jd91k19z 232 27 ) ) -RRB- cord-323428-jd91k19z 232 28 are be VBP cord-323428-jd91k19z 232 29 potent potent JJ cord-323428-jd91k19z 232 30 interferon interferon NN cord-323428-jd91k19z 232 31 antagonists antagonist NNS cord-323428-jd91k19z 233 1 Delayed delayed JJ cord-323428-jd91k19z 233 2 induction induction NN cord-323428-jd91k19z 233 3 of of IN cord-323428-jd91k19z 233 4 proinflammatory proinflammatory JJ cord-323428-jd91k19z 233 5 cytokines cytokine NNS cord-323428-jd91k19z 233 6 and and CC cord-323428-jd91k19z 233 7 suppression suppression NN cord-323428-jd91k19z 233 8 of of IN cord-323428-jd91k19z 233 9 innate innate JJ cord-323428-jd91k19z 233 10 antiviral antiviral JJ cord-323428-jd91k19z 233 11 response response NN cord-323428-jd91k19z 233 12 by by IN cord-323428-jd91k19z 233 13 the the DT cord-323428-jd91k19z 233 14 novel novel JJ cord-323428-jd91k19z 233 15 Middle Middle NNP cord-323428-jd91k19z 233 16 East East NNP cord-323428-jd91k19z 233 17 respiratory respiratory JJ cord-323428-jd91k19z 233 18 syndrome syndrome NN cord-323428-jd91k19z 233 19 coronavirus coronavirus NN cord-323428-jd91k19z 233 20 : : : cord-323428-jd91k19z 233 21 Implications implication NNS cord-323428-jd91k19z 233 22 for for IN cord-323428-jd91k19z 233 23 pathogenesis pathogenesis NN cord-323428-jd91k19z 233 24 and and CC cord-323428-jd91k19z 233 25 treatment treatment NN cord-323428-jd91k19z 234 1 Distinct distinct JJ cord-323428-jd91k19z 234 2 immune immune JJ cord-323428-jd91k19z 234 3 response response NN cord-323428-jd91k19z 234 4 in in IN cord-323428-jd91k19z 234 5 two two CD cord-323428-jd91k19z 234 6 MERS MERS NNP cord-323428-jd91k19z 234 7 - - HYPH cord-323428-jd91k19z 234 8 CoV cov NN cord-323428-jd91k19z 234 9 - - HYPH cord-323428-jd91k19z 234 10 infected infect VBN cord-323428-jd91k19z 234 11 patients patient NNS cord-323428-jd91k19z 235 1 : : : cord-323428-jd91k19z 235 2 Can Can MD cord-323428-jd91k19z 235 3 we -PRON- PRP cord-323428-jd91k19z 235 4 go go VB cord-323428-jd91k19z 235 5 from from IN cord-323428-jd91k19z 235 6 bench bench NN cord-323428-jd91k19z 235 7 to to IN cord-323428-jd91k19z 235 8 bedside bedside NN cord-323428-jd91k19z 235 9 ? ? . cord-323428-jd91k19z 236 1 This this DT cord-323428-jd91k19z 236 2 study study NN cord-323428-jd91k19z 236 3 was be VBD cord-323428-jd91k19z 236 4 supported support VBN cord-323428-jd91k19z 236 5 by by IN cord-323428-jd91k19z 236 6 the the DT cord-323428-jd91k19z 236 7 Deanship Deanship NNP cord-323428-jd91k19z 236 8 of of IN cord-323428-jd91k19z 236 9 Research Research NNP cord-323428-jd91k19z 236 10 at at IN cord-323428-jd91k19z 236 11 Jordan Jordan NNP cord-323428-jd91k19z 236 12 University University NNP cord-323428-jd91k19z 236 13 of of IN cord-323428-jd91k19z 236 14 Science Science NNP cord-323428-jd91k19z 236 15 and and CC cord-323428-jd91k19z 236 16 Technology Technology NNP cord-323428-jd91k19z 237 1 ( ( -LRB- cord-323428-jd91k19z 237 2 Grant Grant NNP cord-323428-jd91k19z 237 3 No No NNP cord-323428-jd91k19z 237 4 . . NN cord-323428-jd91k19z 237 5 275/2014 275/2014 CD cord-323428-jd91k19z 237 6 ) ) -RRB- cord-323428-jd91k19z 237 7 . . . cord-323428-jd91k19z 238 1 MA MA NNP cord-323428-jd91k19z 238 2 and and CC cord-323428-jd91k19z 238 3 MuA MuA NNP cord-323428-jd91k19z 238 4 designed design VBD cord-323428-jd91k19z 238 5 the the DT cord-323428-jd91k19z 238 6 adenovirus adenovirus NN cord-323428-jd91k19z 238 7 vaccine vaccine NN cord-323428-jd91k19z 238 8 for for IN cord-323428-jd91k19z 238 9 the the DT cord-323428-jd91k19z 238 10 MERS MERS NNP cord-323428-jd91k19z 238 11 - - HYPH cord-323428-jd91k19z 238 12 S1 S1 NNP cord-323428-jd91k19z 238 13 vaccine vaccine NN cord-323428-jd91k19z 238 14 . . . cord-323428-jd91k19z 239 1 MA MA NNP cord-323428-jd91k19z 239 2 and and CC cord-323428-jd91k19z 239 3 MK MK NNP cord-323428-jd91k19z 239 4 designed design VBD cord-323428-jd91k19z 239 5 the the DT cord-323428-jd91k19z 239 6 mice mice NN cord-323428-jd91k19z 239 7 experiments experiment NNS cord-323428-jd91k19z 239 8 . . . cord-323428-jd91k19z 240 1 MuA MuA NNP cord-323428-jd91k19z 240 2 , , , cord-323428-jd91k19z 240 3 MA MA NNP cord-323428-jd91k19z 240 4 , , , cord-323428-jd91k19z 240 5 and and CC cord-323428-jd91k19z 240 6 MK MK NNP cord-323428-jd91k19z 240 7 took take VBD cord-323428-jd91k19z 240 8 the the DT cord-323428-jd91k19z 240 9 different different JJ cord-323428-jd91k19z 240 10 tissue tissue NN cord-323428-jd91k19z 240 11 samples sample NNS cord-323428-jd91k19z 240 12 from from IN cord-323428-jd91k19z 240 13 the the DT cord-323428-jd91k19z 240 14 mice mouse NNS cord-323428-jd91k19z 240 15 . . . cord-323428-jd91k19z 241 1 MuA MuA NNP cord-323428-jd91k19z 241 2 performed perform VBD cord-323428-jd91k19z 241 3 the the DT cord-323428-jd91k19z 241 4 molecular molecular JJ cord-323428-jd91k19z 241 5 assays assay NNS cord-323428-jd91k19z 241 6 and and CC cord-323428-jd91k19z 241 7 the the DT cord-323428-jd91k19z 241 8 immunohistochemical immunohistochemical JJ cord-323428-jd91k19z 241 9 staining staining NN cord-323428-jd91k19z 241 10 . . . cord-323428-jd91k19z 242 1 MA MA NNP cord-323428-jd91k19z 242 2 and and CC cord-323428-jd91k19z 242 3 MuA MuA NNP cord-323428-jd91k19z 242 4 prepared prepare VBD cord-323428-jd91k19z 242 5 the the DT cord-323428-jd91k19z 242 6 manuscript manuscript NN cord-323428-jd91k19z 242 7 . . . cord-323428-jd91k19z 243 1 All all DT cord-323428-jd91k19z 243 2 authors author NNS cord-323428-jd91k19z 243 3 read read VBP cord-323428-jd91k19z 243 4 and and CC cord-323428-jd91k19z 243 5 approved approve VBD cord-323428-jd91k19z 243 6 the the DT cord-323428-jd91k19z 243 7 final final JJ cord-323428-jd91k19z 243 8 manuscript manuscript NN cord-323428-jd91k19z 243 9 . . . cord-323428-jd91k19z 244 1 The the DT cord-323428-jd91k19z 244 2 authors author NNS cord-323428-jd91k19z 244 3 declare declare VBP cord-323428-jd91k19z 244 4 that that IN cord-323428-jd91k19z 244 5 they -PRON- PRP cord-323428-jd91k19z 244 6 have have VBP cord-323428-jd91k19z 244 7 no no DT cord-323428-jd91k19z 244 8 competing compete VBG cord-323428-jd91k19z 244 9 interests interest NNS cord-323428-jd91k19z 244 10 . . . cord-323428-jd91k19z 245 1 Veterinary Veterinary NNP cord-323428-jd91k19z 245 2 World World NNP cord-323428-jd91k19z 245 3 remains remain VBZ cord-323428-jd91k19z 245 4 neutral neutral JJ cord-323428-jd91k19z 245 5 with with IN cord-323428-jd91k19z 245 6 regard regard NN cord-323428-jd91k19z 245 7 to to IN cord-323428-jd91k19z 245 8 jurisdictional jurisdictional JJ cord-323428-jd91k19z 245 9 claims claim NNS cord-323428-jd91k19z 245 10 in in IN cord-323428-jd91k19z 245 11 published publish VBN cord-323428-jd91k19z 245 12 institutional institutional JJ cord-323428-jd91k19z 245 13 affiliation affiliation NN cord-323428-jd91k19z 245 14 . . .