id	sid	tid	token	lemma	pos
cord-293966-5c466xvz	1	1	key	key	JJ
cord-293966-5c466xvz	1	2	:	:	:
cord-293966-5c466xvz	1	3	cord-293966	cord-293966	NNP
cord-293966-5c466xvz	1	4	-	-	HYPH
cord-293966-5c466xvz	1	5	5c466xvz	5c466xvz	CD
cord-293966-5c466xvz	1	6	authors	author	NNS
cord-293966-5c466xvz	1	7	:	:	:
cord-293966-5c466xvz	1	8	Fehr	Fehr	NNP
cord-293966-5c466xvz	1	9	,	,	,
cord-293966-5c466xvz	1	10	Anthony	Anthony	NNP
cord-293966-5c466xvz	1	11	R.	R.	NNP
cord-293966-5c466xvz	1	12	title	title	NN
cord-293966-5c466xvz	1	13	:	:	:
cord-293966-5c466xvz	1	14	Bacterial	Bacterial	NNP
cord-293966-5c466xvz	1	15	Artificial	Artificial	NNP
cord-293966-5c466xvz	1	16	Chromosome	Chromosome	NNP
cord-293966-5c466xvz	1	17	-	-	HYPH
cord-293966-5c466xvz	1	18	Based	Based	NNP
cord-293966-5c466xvz	1	19	Lambda	Lambda	NNP
cord-293966-5c466xvz	1	20	Red	Red	NNP
cord-293966-5c466xvz	1	21	Recombination	Recombination	NNP
cord-293966-5c466xvz	1	22	with	with	IN
cord-293966-5c466xvz	1	23	the	the	DT
cord-293966-5c466xvz	1	24	I	I	NNP
cord-293966-5c466xvz	1	25	-	-	HYPH
cord-293966-5c466xvz	1	26	SceI	SceI	NNP
cord-293966-5c466xvz	1	27	Homing	Homing	NNP
cord-293966-5c466xvz	1	28	Endonuclease	Endonuclease	NNP
cord-293966-5c466xvz	1	29	for	for	IN
cord-293966-5c466xvz	1	30	Genetic	genetic	JJ
cord-293966-5c466xvz	1	31	Alteration	Alteration	NNP
cord-293966-5c466xvz	1	32	of	of	IN
cord-293966-5c466xvz	1	33	MERS	MERS	NNP
cord-293966-5c466xvz	1	34	-	-	HYPH
cord-293966-5c466xvz	1	35	CoV	CoV	NNP
cord-293966-5c466xvz	1	36	date	date	NN
cord-293966-5c466xvz	1	37	:	:	:
cord-293966-5c466xvz	1	38	2019	2019	CD
cord-293966-5c466xvz	1	39	-	-	HYPH
cord-293966-5c466xvz	1	40	09	09	CD
cord-293966-5c466xvz	1	41	-	-	SYM
cord-293966-5c466xvz	1	42	14	14	CD
cord-293966-5c466xvz	1	43	journal	journal	NN
cord-293966-5c466xvz	1	44	:	:	:
cord-293966-5c466xvz	1	45	MERS	MERS	NNP
cord-293966-5c466xvz	2	1	Coronavirus	Coronavirus	NNP
cord-293966-5c466xvz	2	2	DOI	DOI	NNP
cord-293966-5c466xvz	2	3	:	:	:
cord-293966-5c466xvz	2	4	10.1007/978	10.1007/978	CD
cord-293966-5c466xvz	2	5	-	-	HYPH
cord-293966-5c466xvz	2	6	1	1	CD
cord-293966-5c466xvz	2	7	-	-	HYPH
cord-293966-5c466xvz	2	8	0716	0716	CD
cord-293966-5c466xvz	3	1	-0211	-0211	:
cord-293966-5c466xvz	3	2	-	-	:
cord-293966-5c466xvz	3	3	9_5	9_5	CD
cord-293966-5c466xvz	4	1	sha	sha	NNP
cord-293966-5c466xvz	4	2	:	:	:
cord-293966-5c466xvz	5	1	7c57bce2fb142a42c410c0ecd1e838012ee79078	7c57bce2fb142a42c410c0ecd1e838012ee79078	CD
cord-293966-5c466xvz	5	2	doc_id	doc_id	CD
cord-293966-5c466xvz	5	3	:	:	:
cord-293966-5c466xvz	5	4	293966	293966	CD
cord-293966-5c466xvz	5	5	cord_uid	cord_uid	NNS
cord-293966-5c466xvz	5	6	:	:	:
cord-293966-5c466xvz	5	7	5c466xvz	5c466xvz	CD
cord-293966-5c466xvz	5	8	Over	over	IN
cord-293966-5c466xvz	5	9	the	the	DT
cord-293966-5c466xvz	5	10	past	past	JJ
cord-293966-5c466xvz	5	11	two	two	CD
cord-293966-5c466xvz	5	12	decades	decade	NNS
cord-293966-5c466xvz	6	1	,	,	,
cord-293966-5c466xvz	6	2	several	several	JJ
cord-293966-5c466xvz	6	3	coronavirus	coronavirus	NN
cord-293966-5c466xvz	6	4	(	(	-LRB-
cord-293966-5c466xvz	6	5	CoV	CoV	NNP
cord-293966-5c466xvz	6	6	)	)	-RRB-
cord-293966-5c466xvz	6	7	infectious	infectious	JJ
cord-293966-5c466xvz	6	8	clones	clone	NNS
cord-293966-5c466xvz	6	9	have	have	VBP
cord-293966-5c466xvz	6	10	been	be	VBN
cord-293966-5c466xvz	6	11	engineered	engineer	VBN
cord-293966-5c466xvz	6	12	,	,	,
cord-293966-5c466xvz	6	13	allowing	allow	VBG
cord-293966-5c466xvz	6	14	for	for	IN
cord-293966-5c466xvz	6	15	the	the	DT
cord-293966-5c466xvz	6	16	manipulation	manipulation	NN
cord-293966-5c466xvz	6	17	of	of	IN
cord-293966-5c466xvz	6	18	their	-PRON-	PRP$
cord-293966-5c466xvz	6	19	large	large	JJ
cord-293966-5c466xvz	6	20	viral	viral	JJ
cord-293966-5c466xvz	6	21	genomes	genome	NNS
cord-293966-5c466xvz	6	22	(	(	-LRB-
cord-293966-5c466xvz	6	23	~30	~30	NNP
cord-293966-5c466xvz	6	24	kb	kb	NNP
cord-293966-5c466xvz	6	25	)	)	-RRB-
cord-293966-5c466xvz	6	26	using	use	VBG
cord-293966-5c466xvz	6	27	unique	unique	JJ
cord-293966-5c466xvz	6	28	reverse	reverse	JJ
cord-293966-5c466xvz	6	29	genetic	genetic	JJ
cord-293966-5c466xvz	6	30	systems	system	NNS
cord-293966-5c466xvz	6	31	.	.	.
cord-293966-5c466xvz	7	1	These	these	DT
cord-293966-5c466xvz	7	2	reverse	reverse	VBP
cord-293966-5c466xvz	7	3	genetic	genetic	JJ
cord-293966-5c466xvz	7	4	systems	system	NNS
cord-293966-5c466xvz	7	5	include	include	VBP
cord-293966-5c466xvz	7	6	targeted	target	VBN
cord-293966-5c466xvz	7	7	recombination	recombination	NN
cord-293966-5c466xvz	7	8	,	,	,
cord-293966-5c466xvz	7	9	in	in	IN
cord-293966-5c466xvz	7	10	vitro	vitro	FW
cord-293966-5c466xvz	7	11	ligation	ligation	NN
cord-293966-5c466xvz	7	12	,	,	,
cord-293966-5c466xvz	7	13	vaccinia	vaccinia	NN
cord-293966-5c466xvz	7	14	virus	virus	NN
cord-293966-5c466xvz	7	15	vectors	vector	NNS
cord-293966-5c466xvz	7	16	,	,	,
cord-293966-5c466xvz	7	17	and	and	CC
cord-293966-5c466xvz	7	18	bacterial	bacterial	JJ
cord-293966-5c466xvz	7	19	artificial	artificial	JJ
cord-293966-5c466xvz	7	20	chromosomes	chromosome	NNS
cord-293966-5c466xvz	7	21	(	(	-LRB-
cord-293966-5c466xvz	7	22	BACs	bac	NNS
cord-293966-5c466xvz	7	23	)	)	-RRB-
cord-293966-5c466xvz	7	24	.	.	.
cord-293966-5c466xvz	8	1	Quickly	quickly	RB
cord-293966-5c466xvz	8	2	after	after	IN
cord-293966-5c466xvz	8	3	the	the	DT
cord-293966-5c466xvz	8	4	identification	identification	NN
cord-293966-5c466xvz	8	5	of	of	IN
cord-293966-5c466xvz	8	6	Middle	Middle	NNP
cord-293966-5c466xvz	8	7	East	East	NNP
cord-293966-5c466xvz	8	8	respiratory	respiratory	JJ
cord-293966-5c466xvz	8	9	syndrome	syndrome	NN
cord-293966-5c466xvz	8	10	-	-	HYPH
cord-293966-5c466xvz	8	11	CoV	cov	NN
cord-293966-5c466xvz	8	12	(	(	-LRB-
cord-293966-5c466xvz	8	13	MERS	MERS	NNP
cord-293966-5c466xvz	8	14	-	-	HYPH
cord-293966-5c466xvz	8	15	CoV	CoV	NNP
cord-293966-5c466xvz	8	16	)	)	-RRB-
cord-293966-5c466xvz	8	17	,	,	,
cord-293966-5c466xvz	8	18	both	both	CC
cord-293966-5c466xvz	8	19	in	in	IN
cord-293966-5c466xvz	8	20	vitro	vitro	FW
cord-293966-5c466xvz	8	21	ligation	ligation	NN
cord-293966-5c466xvz	8	22	and	and	CC
cord-293966-5c466xvz	8	23	BAC	BAC	NNP
cord-293966-5c466xvz	8	24	-	-	HYPH
cord-293966-5c466xvz	8	25	based	base	VBN
cord-293966-5c466xvz	8	26	reverse	reverse	JJ
cord-293966-5c466xvz	8	27	genetic	genetic	JJ
cord-293966-5c466xvz	8	28	technologies	technology	NNS
cord-293966-5c466xvz	8	29	were	be	VBD
cord-293966-5c466xvz	8	30	engineered	engineer	VBN
cord-293966-5c466xvz	8	31	for	for	IN
cord-293966-5c466xvz	8	32	MERS	MERS	NNP
cord-293966-5c466xvz	8	33	-	-	HYPH
cord-293966-5c466xvz	8	34	CoV	CoV	NNP
cord-293966-5c466xvz	8	35	to	to	TO
cord-293966-5c466xvz	8	36	study	study	VB
cord-293966-5c466xvz	8	37	its	-PRON-	PRP$
cord-293966-5c466xvz	8	38	basic	basic	JJ
cord-293966-5c466xvz	8	39	biological	biological	JJ
cord-293966-5c466xvz	8	40	properties	property	NNS
cord-293966-5c466xvz	8	41	,	,	,
cord-293966-5c466xvz	8	42	develop	develop	VB
cord-293966-5c466xvz	8	43	live	live	RB
cord-293966-5c466xvz	8	44	-	-	HYPH
cord-293966-5c466xvz	8	45	attenuated	attenuate	VBN
cord-293966-5c466xvz	8	46	vaccines	vaccine	NNS
cord-293966-5c466xvz	8	47	,	,	,
cord-293966-5c466xvz	8	48	and	and	CC
cord-293966-5c466xvz	8	49	test	test	VBP
cord-293966-5c466xvz	8	50	antiviral	antiviral	JJ
cord-293966-5c466xvz	8	51	drugs	drug	NNS
cord-293966-5c466xvz	8	52	.	.	.
cord-293966-5c466xvz	9	1	Here	here	RB
cord-293966-5c466xvz	9	2	,	,	,
cord-293966-5c466xvz	9	3	I	-PRON-	PRP
cord-293966-5c466xvz	9	4	will	will	MD
cord-293966-5c466xvz	9	5	describe	describe	VB
cord-293966-5c466xvz	9	6	how	how	WRB
cord-293966-5c466xvz	9	7	lambda	lambda	NN
cord-293966-5c466xvz	9	8	red	red	JJ
cord-293966-5c466xvz	9	9	recombination	recombination	NN
cord-293966-5c466xvz	9	10	can	can	MD
cord-293966-5c466xvz	9	11	be	be	VB
cord-293966-5c466xvz	9	12	used	use	VBN
cord-293966-5c466xvz	9	13	with	with	IN
cord-293966-5c466xvz	9	14	the	the	DT
cord-293966-5c466xvz	9	15	MERS	MERS	NNP
cord-293966-5c466xvz	9	16	-	-	HYPH
cord-293966-5c466xvz	9	17	CoV	cov	NN
cord-293966-5c466xvz	9	18	BAC	BAC	NNP
cord-293966-5c466xvz	9	19	to	to	TO
cord-293966-5c466xvz	9	20	quickly	quickly	RB
cord-293966-5c466xvz	9	21	and	and	CC
cord-293966-5c466xvz	9	22	efficiently	efficiently	RB
cord-293966-5c466xvz	9	23	introduce	introduce	VB
cord-293966-5c466xvz	9	24	virtually	virtually	RB
cord-293966-5c466xvz	9	25	any	any	DT
cord-293966-5c466xvz	9	26	type	type	NN
cord-293966-5c466xvz	9	27	of	of	IN
cord-293966-5c466xvz	9	28	genetic	genetic	JJ
cord-293966-5c466xvz	9	29	modification	modification	NN
cord-293966-5c466xvz	9	30	(	(	-LRB-
cord-293966-5c466xvz	9	31	point	point	NN
cord-293966-5c466xvz	9	32	mutations	mutation	NNS
cord-293966-5c466xvz	9	33	,	,	,
cord-293966-5c466xvz	9	34	insertions	insertion	NNS
cord-293966-5c466xvz	9	35	,	,	,
cord-293966-5c466xvz	9	36	deletions	deletion	NNS
cord-293966-5c466xvz	9	37	)	)	-RRB-
cord-293966-5c466xvz	9	38	into	into	IN
cord-293966-5c466xvz	9	39	the	the	DT
cord-293966-5c466xvz	9	40	MERS	MERS	NNP
cord-293966-5c466xvz	9	41	-	-	HYPH
cord-293966-5c466xvz	9	42	CoV	CoV	NNP
cord-293966-5c466xvz	9	43	genome	genome	NN
cord-293966-5c466xvz	9	44	and	and	CC
cord-293966-5c466xvz	9	45	recover	recover	VB
cord-293966-5c466xvz	9	46	recombinant	recombinant	JJ
cord-293966-5c466xvz	9	47	virus	virus	NN
cord-293966-5c466xvz	9	48	.	.	.
cord-293966-5c466xvz	10	1	Coronaviruses	coronaviruse	NNS
cord-293966-5c466xvz	10	2	are	be	VBP
cord-293966-5c466xvz	10	3	large	large	JJ
cord-293966-5c466xvz	10	4	,	,	,
cord-293966-5c466xvz	10	5	enveloped	envelop	VBN
cord-293966-5c466xvz	10	6	,	,	,
cord-293966-5c466xvz	10	7	single	single	RB
cord-293966-5c466xvz	10	8	-	-	HYPH
cord-293966-5c466xvz	10	9	stranded	strand	VBN
cord-293966-5c466xvz	10	10	positive	positive	JJ
cord-293966-5c466xvz	10	11	-	-	HYPH
cord-293966-5c466xvz	10	12	sense	sense	NN
cord-293966-5c466xvz	10	13	RNA	RNA	NNP
cord-293966-5c466xvz	10	14	viruses	virus	NNS
cord-293966-5c466xvz	10	15	that	that	WDT
cord-293966-5c466xvz	10	16	cause	cause	VBP
cord-293966-5c466xvz	10	17	both	both	DT
cord-293966-5c466xvz	10	18	significant	significant	JJ
cord-293966-5c466xvz	10	19	human	human	JJ
cord-293966-5c466xvz	10	20	and	and	CC
cord-293966-5c466xvz	10	21	veterinary	veterinary	JJ
cord-293966-5c466xvz	10	22	disease	disease	NN
cord-293966-5c466xvz	10	23	.	.	.
cord-293966-5c466xvz	11	1	Prior	prior	RB
cord-293966-5c466xvz	11	2	to	to	IN
cord-293966-5c466xvz	11	3	the	the	DT
cord-293966-5c466xvz	11	4	severe	severe	JJ
cord-293966-5c466xvz	11	5	acute	acute	JJ
cord-293966-5c466xvz	11	6	respiratory	respiratory	JJ
cord-293966-5c466xvz	11	7	syndrome	syndrome	NN
cord-293966-5c466xvz	11	8	-	-	HYPH
cord-293966-5c466xvz	11	9	CoV	CoV	NNP
cord-293966-5c466xvz	11	10	(	(	-LRB-
cord-293966-5c466xvz	11	11	SARS	SARS	NNP
cord-293966-5c466xvz	11	12	-	-	HYPH
cord-293966-5c466xvz	11	13	CoV	CoV	NNP
cord-293966-5c466xvz	11	14	)	)	-RRB-
cord-293966-5c466xvz	11	15	outbreak	outbreak	NN
cord-293966-5c466xvz	11	16	in	in	IN
cord-293966-5c466xvz	11	17	2003	2003	CD
cord-293966-5c466xvz	11	18	,	,	,
cord-293966-5c466xvz	11	19	human	human	JJ
cord-293966-5c466xvz	11	20	CoVs	CoVs	NNP
cord-293966-5c466xvz	11	21	were	be	VBD
cord-293966-5c466xvz	11	22	only	only	RB
cord-293966-5c466xvz	11	23	known	know	VBN
cord-293966-5c466xvz	11	24	to	to	TO
cord-293966-5c466xvz	11	25	cause	cause	VB
cord-293966-5c466xvz	11	26	mild	mild	JJ
cord-293966-5c466xvz	11	27	,	,	,
cord-293966-5c466xvz	11	28	self	self	NN
cord-293966-5c466xvz	11	29	-	-	HYPH
cord-293966-5c466xvz	11	30	limiting	limit	VBG
cord-293966-5c466xvz	11	31	upper	upper	JJ
cord-293966-5c466xvz	11	32	respiratory	respiratory	JJ
cord-293966-5c466xvz	11	33	diseases	disease	NNS
cord-293966-5c466xvz	11	34	.	.	.
cord-293966-5c466xvz	12	1	Approximately	approximately	RB
cord-293966-5c466xvz	12	2	10	10	CD
cord-293966-5c466xvz	12	3	years	year	NNS
cord-293966-5c466xvz	12	4	after	after	IN
cord-293966-5c466xvz	12	5	the	the	DT
cord-293966-5c466xvz	12	6	emergence	emergence	NN
cord-293966-5c466xvz	12	7	of	of	IN
cord-293966-5c466xvz	12	8	SARS	SARS	NNP
cord-293966-5c466xvz	12	9	-	-	HYPH
cord-293966-5c466xvz	12	10	CoV	CoV	NNP
cord-293966-5c466xvz	12	11	in	in	IN
cord-293966-5c466xvz	12	12	2012	2012	CD
cord-293966-5c466xvz	12	13	,	,	,
cord-293966-5c466xvz	12	14	Middle	Middle	NNP
cord-293966-5c466xvz	12	15	East	East	NNP
cord-293966-5c466xvz	12	16	respiratory	respiratory	JJ
cord-293966-5c466xvz	12	17	syndrome	syndrome	NN
cord-293966-5c466xvz	12	18	(	(	-LRB-
cord-293966-5c466xvz	12	19	MERS)-CoV	MERS)-CoV	NNP
cord-293966-5c466xvz	12	20	emerged	emerge	VBD
cord-293966-5c466xvz	12	21	in	in	IN
cord-293966-5c466xvz	12	22	the	the	DT
cord-293966-5c466xvz	12	23	Middle	Middle	NNP
cord-293966-5c466xvz	12	24	East	East	NNP
cord-293966-5c466xvz	12	25	where	where	WRB
cord-293966-5c466xvz	12	26	it	-PRON-	PRP
cord-293966-5c466xvz	12	27	then	then	RB
cord-293966-5c466xvz	12	28	spread	spread	VBD
cord-293966-5c466xvz	12	29	to	to	IN
cord-293966-5c466xvz	12	30	27	27	CD
cord-293966-5c466xvz	12	31	different	different	JJ
cord-293966-5c466xvz	12	32	countries	country	NNS
cord-293966-5c466xvz	12	33	,	,	,
cord-293966-5c466xvz	12	34	and	and	CC
cord-293966-5c466xvz	12	35	to	to	IN
cord-293966-5c466xvz	12	36	date	date	NN
cord-293966-5c466xvz	12	37	(	(	-LRB-
cord-293966-5c466xvz	12	38	December	December	NNP
cord-293966-5c466xvz	12	39	2018	2018	CD
cord-293966-5c466xvz	12	40	,	,	,
cord-293966-5c466xvz	12	41	WHO	who	WP
cord-293966-5c466xvz	12	42	)	)	-RRB-
cord-293966-5c466xvz	12	43	there	there	EX
cord-293966-5c466xvz	12	44	have	have	VBP
cord-293966-5c466xvz	12	45	been	be	VBN
cord-293966-5c466xvz	12	46	2278	2278	CD
cord-293966-5c466xvz	12	47	laboratoryconfirmed	laboratoryconfirme	VBN
cord-293966-5c466xvz	12	48	cases	case	NNS
cord-293966-5c466xvz	12	49	and	and	CC
cord-293966-5c466xvz	12	50	806	806	CD
cord-293966-5c466xvz	12	51	associated	associate	VBN
cord-293966-5c466xvz	12	52	deaths	death	NNS
cord-293966-5c466xvz	12	53	for	for	IN
cord-293966-5c466xvz	12	54	a	a	DT
cord-293966-5c466xvz	12	55	case	case	NN
cord-293966-5c466xvz	12	56	fatality	fatality	NN
cord-293966-5c466xvz	12	57	rate	rate	NN
cord-293966-5c466xvz	12	58	of	of	IN
cord-293966-5c466xvz	12	59	35	35	CD
cord-293966-5c466xvz	12	60	%	%	NN
cord-293966-5c466xvz	12	61	.	.	.
cord-293966-5c466xvz	13	1	Most	Most	JJS
cord-293966-5c466xvz	13	2	of	of	IN
cord-293966-5c466xvz	13	3	these	these	DT
cord-293966-5c466xvz	13	4	cases	case	NNS
cord-293966-5c466xvz	13	5	have	have	VBP
cord-293966-5c466xvz	13	6	occurred	occur	VBN
cord-293966-5c466xvz	13	7	in	in	IN
cord-293966-5c466xvz	13	8	the	the	DT
cord-293966-5c466xvz	13	9	Middle	Middle	NNP
cord-293966-5c466xvz	13	10	East	East	NNP
cord-293966-5c466xvz	13	11	,	,	,
cord-293966-5c466xvz	13	12	aside	aside	RB
cord-293966-5c466xvz	13	13	from	from	IN
cord-293966-5c466xvz	13	14	an	an	DT
cord-293966-5c466xvz	13	15	outbreak	outbreak	NN
cord-293966-5c466xvz	13	16	of~200	of~200	ADD
cord-293966-5c466xvz	13	17	infected	infect	VBN
cord-293966-5c466xvz	13	18	individuals	individual	NNS
cord-293966-5c466xvz	13	19	in	in	IN
cord-293966-5c466xvz	13	20	South	South	NNP
cord-293966-5c466xvz	13	21	Korea	Korea	NNP
cord-293966-5c466xvz	13	22	in	in	IN
cord-293966-5c466xvz	13	23	2015	2015	CD
cord-293966-5c466xvz	13	24	[	[	-LRB-
cord-293966-5c466xvz	13	25	1	1	CD
cord-293966-5c466xvz	13	26	]	]	-RRB-
cord-293966-5c466xvz	13	27	.	.	.
cord-293966-5c466xvz	14	1	Infectious	infectious	JJ
cord-293966-5c466xvz	14	2	clones	clone	NNS
cord-293966-5c466xvz	14	3	are	be	VBP
cord-293966-5c466xvz	14	4	highly	highly	RB
cord-293966-5c466xvz	14	5	valuable	valuable	JJ
cord-293966-5c466xvz	14	6	research	research	NN
cord-293966-5c466xvz	14	7	tools	tool	NNS
cord-293966-5c466xvz	14	8	that	that	WDT
cord-293966-5c466xvz	14	9	enable	enable	VBP
cord-293966-5c466xvz	14	10	modification	modification	NN
cord-293966-5c466xvz	14	11	of	of	IN
cord-293966-5c466xvz	14	12	viral	viral	JJ
cord-293966-5c466xvz	14	13	genomes	genome	NNS
cord-293966-5c466xvz	14	14	to	to	TO
cord-293966-5c466xvz	14	15	better	well	RBR
cord-293966-5c466xvz	14	16	understand	understand	VB
cord-293966-5c466xvz	14	17	their	-PRON-	PRP$
cord-293966-5c466xvz	14	18	fundamental	fundamental	JJ
cord-293966-5c466xvz	14	19	biology	biology	NN
cord-293966-5c466xvz	14	20	,	,	,
cord-293966-5c466xvz	14	21	develop	develop	VB
cord-293966-5c466xvz	14	22	novel	novel	JJ
cord-293966-5c466xvz	14	23	vaccine	vaccine	NN
cord-293966-5c466xvz	14	24	candidates	candidate	NNS
cord-293966-5c466xvz	14	25	,	,	,
cord-293966-5c466xvz	14	26	and	and	CC
cord-293966-5c466xvz	14	27	test	test	VBP
cord-293966-5c466xvz	14	28	antiviral	antiviral	JJ
cord-293966-5c466xvz	14	29	therapeutics	therapeutic	NNS
cord-293966-5c466xvz	14	30	.	.	.
cord-293966-5c466xvz	15	1	Soon	soon	RB
cord-293966-5c466xvz	15	2	after	after	IN
cord-293966-5c466xvz	15	3	identifying	identify	VBG
cord-293966-5c466xvz	15	4	MERS	MERS	NNP
cord-293966-5c466xvz	15	5	-	-	HYPH
cord-293966-5c466xvz	15	6	CoV	CoV	NNP
cord-293966-5c466xvz	15	7	as	as	IN
cord-293966-5c466xvz	15	8	the	the	DT
cord-293966-5c466xvz	15	9	causative	causative	JJ
cord-293966-5c466xvz	15	10	agent	agent	NN
cord-293966-5c466xvz	15	11	of	of	IN
cord-293966-5c466xvz	15	12	MERS	MERS	NNP
cord-293966-5c466xvz	15	13	,	,	,
cord-293966-5c466xvz	15	14	two	two	CD
cord-293966-5c466xvz	15	15	distinct	distinct	JJ
cord-293966-5c466xvz	15	16	infectious	infectious	JJ
cord-293966-5c466xvz	15	17	clones	clone	NNS
cord-293966-5c466xvz	15	18	were	be	VBD
cord-293966-5c466xvz	15	19	reported	report	VBN
cord-293966-5c466xvz	15	20	for	for	IN
cord-293966-5c466xvz	15	21	MERS	MERS	NNP
cord-293966-5c466xvz	15	22	-	-	HYPH
cord-293966-5c466xvz	15	23	CoV	CoV	NNP
cord-293966-5c466xvz	15	24	[	[	-LRB-
cord-293966-5c466xvz	15	25	2	2	CD
cord-293966-5c466xvz	15	26	,	,	,
cord-293966-5c466xvz	15	27	3	3	CD
cord-293966-5c466xvz	15	28	]	]	-RRB-
cord-293966-5c466xvz	15	29	.	.	.
cord-293966-5c466xvz	16	1	These	these	DT
cord-293966-5c466xvz	16	2	infectious	infectious	JJ
cord-293966-5c466xvz	16	3	clones	clone	NNS
cord-293966-5c466xvz	16	4	were	be	VBD
cord-293966-5c466xvz	16	5	engineered	engineer	VBN
cord-293966-5c466xvz	16	6	using	use	VBG
cord-293966-5c466xvz	16	7	in	in	FW
cord-293966-5c466xvz	16	8	vitro	vitro	FW
cord-293966-5c466xvz	16	9	ligation	ligation	NN
cord-293966-5c466xvz	16	10	or	or	CC
cord-293966-5c466xvz	16	11	bacterial	bacterial	JJ
cord-293966-5c466xvz	16	12	artificial	artificial	JJ
cord-293966-5c466xvz	16	13	chromosomes	chromosome	NNS
cord-293966-5c466xvz	16	14	(	(	-LRB-
cord-293966-5c466xvz	16	15	BACs	bac	NNS
cord-293966-5c466xvz	16	16	)	)	-RRB-
cord-293966-5c466xvz	16	17	,	,	,
cord-293966-5c466xvz	16	18	each	each	DT
cord-293966-5c466xvz	16	19	of	of	IN
cord-293966-5c466xvz	16	20	which	which	WDT
cord-293966-5c466xvz	16	21	had	have	VBD
cord-293966-5c466xvz	16	22	been	be	VBN
cord-293966-5c466xvz	16	23	used	use	VBN
cord-293966-5c466xvz	16	24	previously	previously	RB
cord-293966-5c466xvz	16	25	for	for	IN
cord-293966-5c466xvz	16	26	CoVs	CoVs	NNP
cord-293966-5c466xvz	16	27	[	[	-LRB-
cord-293966-5c466xvz	16	28	4	4	CD
cord-293966-5c466xvz	16	29	]	]	-RRB-
cord-293966-5c466xvz	16	30	[	[	-LRB-
cord-293966-5c466xvz	16	31	5	5	CD
cord-293966-5c466xvz	16	32	]	]	-RRB-
cord-293966-5c466xvz	16	33	[	[	-LRB-
cord-293966-5c466xvz	16	34	6	6	CD
cord-293966-5c466xvz	16	35	]	]	-RRB-
cord-293966-5c466xvz	16	36	.	.	.
cord-293966-5c466xvz	17	1	In	in	IN
cord-293966-5c466xvz	17	2	vitro	vitro	FW
cord-293966-5c466xvz	17	3	ligation	ligation	NN
cord-293966-5c466xvz	17	4	uses	use	VBZ
cord-293966-5c466xvz	17	5	unique	unique	JJ
cord-293966-5c466xvz	17	6	type	type	NN
cord-293966-5c466xvz	17	7	II	ii	CD
cord-293966-5c466xvz	17	8	restriction	restriction	NN
cord-293966-5c466xvz	17	9	endonucleases	endonuclease	NNS
cord-293966-5c466xvz	17	10	that	that	WDT
cord-293966-5c466xvz	17	11	cleave	cleave	VBP
cord-293966-5c466xvz	17	12	several	several	JJ
cord-293966-5c466xvz	17	13	bases	basis	NNS
cord-293966-5c466xvz	17	14	away	away	RB
cord-293966-5c466xvz	17	15	from	from	IN
cord-293966-5c466xvz	17	16	their	-PRON-	PRP$
cord-293966-5c466xvz	17	17	recognition	recognition	NN
cord-293966-5c466xvz	17	18	site	site	NN
cord-293966-5c466xvz	17	19	,	,	,
cord-293966-5c466xvz	17	20	allowing	allow	VBG
cord-293966-5c466xvz	17	21	for	for	IN
cord-293966-5c466xvz	17	22	the	the	DT
cord-293966-5c466xvz	17	23	reassembly	reassembly	NN
cord-293966-5c466xvz	17	24	of	of	IN
cord-293966-5c466xvz	17	25	authentic	authentic	JJ
cord-293966-5c466xvz	17	26	CoV	cov	NN
cord-293966-5c466xvz	17	27	genomes	genome	NNS
cord-293966-5c466xvz	17	28	from	from	IN
cord-293966-5c466xvz	17	29	smaller	small	JJR
cord-293966-5c466xvz	17	30	fragments	fragment	NNS
cord-293966-5c466xvz	17	31	.	.	.
cord-293966-5c466xvz	18	1	Each	each	DT
cord-293966-5c466xvz	18	2	fragment	fragment	NN
cord-293966-5c466xvz	18	3	is	be	VBZ
cord-293966-5c466xvz	18	4	separately	separately	RB
cord-293966-5c466xvz	18	5	maintained	maintain	VBN
cord-293966-5c466xvz	18	6	in	in	IN
cord-293966-5c466xvz	18	7	its	-PRON-	PRP$
cord-293966-5c466xvz	18	8	own	own	JJ
cord-293966-5c466xvz	18	9	small	small	JJ
cord-293966-5c466xvz	18	10	plasmid	plasmid	NN
cord-293966-5c466xvz	18	11	for	for	IN
cord-293966-5c466xvz	18	12	efficient	efficient	JJ
cord-293966-5c466xvz	18	13	genetic	genetic	JJ
cord-293966-5c466xvz	18	14	modification	modification	NN
cord-293966-5c466xvz	18	15	using	use	VBG
cord-293966-5c466xvz	18	16	traditional	traditional	JJ
cord-293966-5c466xvz	18	17	molecular	molecular	JJ
cord-293966-5c466xvz	18	18	cloning	cloning	NN
cord-293966-5c466xvz	18	19	methods	method	NNS
cord-293966-5c466xvz	18	20	.	.	.
cord-293966-5c466xvz	19	1	Separating	separate	VBG
cord-293966-5c466xvz	19	2	specific	specific	JJ
cord-293966-5c466xvz	19	3	nucleotide	nucleotide	JJ
cord-293966-5c466xvz	19	4	sequences	sequence	NNS
cord-293966-5c466xvz	19	5	in	in	IN
cord-293966-5c466xvz	19	6	ORF1A	ORF1A	NNP
cord-293966-5c466xvz	19	7	helped	help	VBD
cord-293966-5c466xvz	19	8	to	to	TO
cord-293966-5c466xvz	19	9	eliminate	eliminate	VB
cord-293966-5c466xvz	19	10	the	the	DT
cord-293966-5c466xvz	19	11	problem	problem	NN
cord-293966-5c466xvz	19	12	of	of	IN
cord-293966-5c466xvz	19	13	these	these	DT
cord-293966-5c466xvz	19	14	sequences	sequence	NNS
cord-293966-5c466xvz	19	15	being	be	VBG
cord-293966-5c466xvz	19	16	toxic	toxic	JJ
cord-293966-5c466xvz	19	17	for	for	IN
cord-293966-5c466xvz	19	18	bacteria	bacteria	NNS
cord-293966-5c466xvz	19	19	.	.	.
cord-293966-5c466xvz	20	1	A	a	DT
cord-293966-5c466xvz	20	2	T7	t7	NN
cord-293966-5c466xvz	20	3	promoter	promoter	NN
cord-293966-5c466xvz	20	4	is	be	VBZ
cord-293966-5c466xvz	20	5	inserted	insert	VBN
cord-293966-5c466xvz	20	6	at	at	IN
cord-293966-5c466xvz	20	7	the	the	DT
cord-293966-5c466xvz	20	8	5	5	CD
cord-293966-5c466xvz	20	9	0	0	CD
cord-293966-5c466xvz	20	10	end	end	NN
cord-293966-5c466xvz	20	11	of	of	IN
cord-293966-5c466xvz	20	12	the	the	DT
cord-293966-5c466xvz	20	13	genome	genome	NN
cord-293966-5c466xvz	20	14	,	,	,
cord-293966-5c466xvz	20	15	allowing	allow	VBG
cord-293966-5c466xvz	20	16	for	for	IN
cord-293966-5c466xvz	20	17	in	in	FW
cord-293966-5c466xvz	20	18	vitro	vitro	FW
cord-293966-5c466xvz	20	19	transcription	transcription	NN
cord-293966-5c466xvz	20	20	of	of	IN
cord-293966-5c466xvz	20	21	the	the	DT
cord-293966-5c466xvz	20	22	viral	viral	JJ
cord-293966-5c466xvz	20	23	RNA	RNA	NNP
cord-293966-5c466xvz	20	24	and	and	CC
cord-293966-5c466xvz	20	25	subsequent	subsequent	JJ
cord-293966-5c466xvz	20	26	transfection	transfection	NN
cord-293966-5c466xvz	20	27	into	into	IN
cord-293966-5c466xvz	20	28	mammalian	mammalian	JJ
cord-293966-5c466xvz	20	29	cells	cell	NNS
cord-293966-5c466xvz	20	30	for	for	IN
cord-293966-5c466xvz	20	31	virus	virus	NN
cord-293966-5c466xvz	20	32	production	production	NN
cord-293966-5c466xvz	20	33	.	.	.
cord-293966-5c466xvz	21	1	In	in	IN
cord-293966-5c466xvz	21	2	contrast	contrast	NN
cord-293966-5c466xvz	21	3	,	,	,
cord-293966-5c466xvz	21	4	BACs	bac	NNS
cord-293966-5c466xvz	21	5	allow	allow	VBP
cord-293966-5c466xvz	21	6	for	for	IN
cord-293966-5c466xvz	21	7	the	the	DT
cord-293966-5c466xvz	21	8	stable	stable	JJ
cord-293966-5c466xvz	21	9	propagation	propagation	NN
cord-293966-5c466xvz	21	10	of	of	IN
cord-293966-5c466xvz	21	11	full	full	JJ
cord-293966-5c466xvz	21	12	-	-	HYPH
cord-293966-5c466xvz	21	13	length	length	NN
cord-293966-5c466xvz	21	14	CoV	cov	NN
cord-293966-5c466xvz	21	15	cDNA	cdna	NN
cord-293966-5c466xvz	21	16	in	in	IN
cord-293966-5c466xvz	21	17	bacteria	bacteria	NNS
cord-293966-5c466xvz	21	18	,	,	,
cord-293966-5c466xvz	21	19	due	due	IN
cord-293966-5c466xvz	21	20	to	to	IN
cord-293966-5c466xvz	21	21	the	the	DT
cord-293966-5c466xvz	21	22	ability	ability	NN
cord-293966-5c466xvz	21	23	to	to	TO
cord-293966-5c466xvz	21	24	restrict	restrict	VB
cord-293966-5c466xvz	21	25	their	-PRON-	PRP$
cord-293966-5c466xvz	21	26	copy	copy	NN
cord-293966-5c466xvz	21	27	number	number	NN
cord-293966-5c466xvz	21	28	to	to	IN
cord-293966-5c466xvz	21	29	1	1	CD
cord-293966-5c466xvz	21	30	or	or	CC
cord-293966-5c466xvz	21	31	2	2	CD
cord-293966-5c466xvz	21	32	plasmids	plasmid	NNS
cord-293966-5c466xvz	21	33	per	per	IN
cord-293966-5c466xvz	21	34	cell	cell	NN
cord-293966-5c466xvz	21	35	.	.	.
cord-293966-5c466xvz	22	1	Different	different	JJ
cord-293966-5c466xvz	22	2	restriction	restriction	NN
cord-293966-5c466xvz	22	3	fragments	fragment	NNS
cord-293966-5c466xvz	22	4	of	of	IN
cord-293966-5c466xvz	22	5	these	these	DT
cord-293966-5c466xvz	22	6	BACs	bac	NNS
cord-293966-5c466xvz	22	7	can	can	MD
cord-293966-5c466xvz	22	8	be	be	VB
cord-293966-5c466xvz	22	9	sub	sub	NN
cord-293966-5c466xvz	22	10	-	-	VBN
cord-293966-5c466xvz	22	11	cloned	clone	VBN
cord-293966-5c466xvz	22	12	into	into	IN
cord-293966-5c466xvz	22	13	smaller	small	JJR
cord-293966-5c466xvz	22	14	vectors	vector	NNS
cord-293966-5c466xvz	22	15	for	for	IN
cord-293966-5c466xvz	22	16	efficient	efficient	JJ
cord-293966-5c466xvz	22	17	modification	modification	NN
cord-293966-5c466xvz	22	18	,	,	,
cord-293966-5c466xvz	22	19	or	or	CC
cord-293966-5c466xvz	22	20	the	the	DT
cord-293966-5c466xvz	22	21	full	full	JJ
cord-293966-5c466xvz	22	22	-	-	HYPH
cord-293966-5c466xvz	22	23	length	length	NN
cord-293966-5c466xvz	22	24	genome	genome	NN
cord-293966-5c466xvz	22	25	can	can	MD
cord-293966-5c466xvz	22	26	be	be	VB
cord-293966-5c466xvz	22	27	modified	modify	VBN
cord-293966-5c466xvz	22	28	using	use	VBG
cord-293966-5c466xvz	22	29	lambda	lambda	NN
cord-293966-5c466xvz	22	30	red	red	JJ
cord-293966-5c466xvz	22	31	recombination	recombination	NN
cord-293966-5c466xvz	22	32	,	,	,
cord-293966-5c466xvz	22	33	which	which	WDT
cord-293966-5c466xvz	22	34	will	will	MD
cord-293966-5c466xvz	22	35	be	be	VB
cord-293966-5c466xvz	22	36	discussed	discuss	VBN
cord-293966-5c466xvz	22	37	here	here	RB
cord-293966-5c466xvz	22	38	.	.	.
cord-293966-5c466xvz	23	1	CoV	CoV	NNP
cord-293966-5c466xvz	23	2	BAC	BAC	NNP
cord-293966-5c466xvz	23	3	plasmids	plasmid	NNS
cord-293966-5c466xvz	23	4	contain	contain	VBP
cord-293966-5c466xvz	23	5	a	a	DT
cord-293966-5c466xvz	23	6	CMV	CMV	NNP
cord-293966-5c466xvz	23	7	promoter	promoter	NN
cord-293966-5c466xvz	23	8	5	5	CD
cord-293966-5c466xvz	23	9	0	0	CD
cord-293966-5c466xvz	23	10	of	of	IN
cord-293966-5c466xvz	23	11	the	the	DT
cord-293966-5c466xvz	23	12	viral	viral	JJ
cord-293966-5c466xvz	23	13	genome	genome	NN
cord-293966-5c466xvz	23	14	,	,	,
cord-293966-5c466xvz	23	15	allowing	allow	VBG
cord-293966-5c466xvz	23	16	for	for	IN
cord-293966-5c466xvz	23	17	transcription	transcription	NN
cord-293966-5c466xvz	23	18	of	of	IN
cord-293966-5c466xvz	23	19	the	the	DT
cord-293966-5c466xvz	23	20	viral	viral	JJ
cord-293966-5c466xvz	23	21	genome	genome	NN
cord-293966-5c466xvz	23	22	following	follow	VBG
cord-293966-5c466xvz	23	23	transfection	transfection	NN
cord-293966-5c466xvz	23	24	of	of	IN
cord-293966-5c466xvz	23	25	BAC	BAC	NNP
cord-293966-5c466xvz	23	26	DNA	DNA	NNP
cord-293966-5c466xvz	23	27	into	into	IN
cord-293966-5c466xvz	23	28	mammalian	mammalian	JJ
cord-293966-5c466xvz	23	29	cells	cell	NNS
cord-293966-5c466xvz	23	30	.	.	.
cord-293966-5c466xvz	24	1	In	in	IN
cord-293966-5c466xvz	24	2	addition	addition	NN
cord-293966-5c466xvz	24	3	,	,	,
cord-293966-5c466xvz	24	4	the	the	DT
cord-293966-5c466xvz	24	5	CoV	cov	NN
cord-293966-5c466xvz	24	6	BACs	bac	NNS
cord-293966-5c466xvz	24	7	contain	contain	VBP
cord-293966-5c466xvz	24	8	a	a	DT
cord-293966-5c466xvz	24	9	polyA	polyA	NNP
cord-293966-5c466xvz	24	10	tail	tail	NN
cord-293966-5c466xvz	24	11	,	,	,
cord-293966-5c466xvz	24	12	a	a	DT
cord-293966-5c466xvz	24	13	Hepatitis	Hepatitis	NNP
cord-293966-5c466xvz	24	14	D	d	NN
cord-293966-5c466xvz	24	15	Virus	virus	NN
cord-293966-5c466xvz	24	16	(	(	-LRB-
cord-293966-5c466xvz	24	17	HDV	HDV	NNP
cord-293966-5c466xvz	24	18	)	)	-RRB-
cord-293966-5c466xvz	24	19	ribozyme	ribozyme	NN
cord-293966-5c466xvz	24	20	,	,	,
cord-293966-5c466xvz	24	21	and	and	CC
cord-293966-5c466xvz	24	22	bovine	bovine	JJ
cord-293966-5c466xvz	24	23	growth	growth	NN
cord-293966-5c466xvz	24	24	hormone	hormone	NN
cord-293966-5c466xvz	24	25	(	(	-LRB-
cord-293966-5c466xvz	25	1	BGH	BGH	NNP
cord-293966-5c466xvz	25	2	)	)	-RRB-
cord-293966-5c466xvz	25	3	termination	termination	NN
cord-293966-5c466xvz	25	4	and	and	CC
cord-293966-5c466xvz	25	5	polyadenylation	polyadenylation	NN
cord-293966-5c466xvz	25	6	signals	signal	NNS
cord-293966-5c466xvz	25	7	to	to	TO
cord-293966-5c466xvz	25	8	create	create	VB
cord-293966-5c466xvz	25	9	genomic	genomic	JJ
cord-293966-5c466xvz	25	10	RNA	RNA	NNP
cord-293966-5c466xvz	25	11	with	with	IN
cord-293966-5c466xvz	25	12	an	an	DT
cord-293966-5c466xvz	25	13	authentic	authentic	JJ
cord-293966-5c466xvz	25	14	3	3	CD
cord-293966-5c466xvz	25	15	0	0	CD
cord-293966-5c466xvz	25	16	end	end	NN
cord-293966-5c466xvz	25	17	.	.	.
cord-293966-5c466xvz	26	1	The	the	DT
cord-293966-5c466xvz	26	2	full	full	JJ
cord-293966-5c466xvz	26	3	-	-	HYPH
cord-293966-5c466xvz	26	4	length	length	NN
cord-293966-5c466xvz	26	5	nature	nature	NN
cord-293966-5c466xvz	26	6	of	of	IN
cord-293966-5c466xvz	26	7	BAC	BAC	NNP
cord-293966-5c466xvz	26	8	DNA	DNA	NNP
cord-293966-5c466xvz	26	9	and	and	CC
cord-293966-5c466xvz	26	10	the	the	DT
cord-293966-5c466xvz	26	11	CMV	CMV	NNP
cord-293966-5c466xvz	26	12	promoter	promoter	NN
cord-293966-5c466xvz	26	13	subvert	subvert	VBP
cord-293966-5c466xvz	26	14	the	the	DT
cord-293966-5c466xvz	26	15	need	need	NN
cord-293966-5c466xvz	26	16	for	for	IN
cord-293966-5c466xvz	26	17	in	in	FW
cord-293966-5c466xvz	26	18	vitro	vitro	FW
cord-293966-5c466xvz	26	19	ligation	ligation	NN
cord-293966-5c466xvz	26	20	or	or	CC
cord-293966-5c466xvz	26	21	transcription	transcription	NN
cord-293966-5c466xvz	26	22	to	to	TO
cord-293966-5c466xvz	26	23	recover	recover	VB
cord-293966-5c466xvz	26	24	infectious	infectious	JJ
cord-293966-5c466xvz	26	25	virus	virus	NN
cord-293966-5c466xvz	26	26	.	.	.
cord-293966-5c466xvz	27	1	BACs	bac	NNS
cord-293966-5c466xvz	27	2	were	be	VBD
cord-293966-5c466xvz	27	3	initially	initially	RB
cord-293966-5c466xvz	27	4	developed	develop	VBN
cord-293966-5c466xvz	27	5	in	in	IN
cord-293966-5c466xvz	27	6	the	the	DT
cord-293966-5c466xvz	27	7	early	early	JJ
cord-293966-5c466xvz	27	8	1990s	1990	NNS
cord-293966-5c466xvz	27	9	,	,	,
cord-293966-5c466xvz	27	10	and	and	CC
cord-293966-5c466xvz	27	11	by	by	IN
cord-293966-5c466xvz	27	12	the	the	DT
cord-293966-5c466xvz	27	13	mid	mid	JJ
cord-293966-5c466xvz	27	14	-	-	JJ
cord-293966-5c466xvz	27	15	late	late	JJ
cord-293966-5c466xvz	27	16	1990s	1990	NNS
cord-293966-5c466xvz	27	17	they	-PRON-	PRP
cord-293966-5c466xvz	27	18	were	be	VBD
cord-293966-5c466xvz	27	19	utilized	utilize	VBN
cord-293966-5c466xvz	27	20	by	by	IN
cord-293966-5c466xvz	27	21	Herpes	Herpes	NNP
cord-293966-5c466xvz	27	22	virologists	virologist	NNS
cord-293966-5c466xvz	27	23	for	for	IN
cord-293966-5c466xvz	27	24	modification	modification	NN
cord-293966-5c466xvz	27	25	of	of	IN
cord-293966-5c466xvz	27	26	these	these	DT
cord-293966-5c466xvz	27	27	large	large	JJ
cord-293966-5c466xvz	27	28	DNA	dna	NN
cord-293966-5c466xvz	27	29	viruses	virus	NNS
cord-293966-5c466xvz	27	30	,	,	,
cord-293966-5c466xvz	27	31	which	which	WDT
cord-293966-5c466xvz	27	32	revolutionized	revolutionize	VBD
cord-293966-5c466xvz	27	33	the	the	DT
cord-293966-5c466xvz	27	34	field	field	NN
cord-293966-5c466xvz	27	35	.	.	.
cord-293966-5c466xvz	28	1	A	a	DT
cord-293966-5c466xvz	28	2	few	few	JJ
cord-293966-5c466xvz	28	3	years	year	NNS
cord-293966-5c466xvz	28	4	later	later	RB
cord-293966-5c466xvz	28	5	a	a	DT
cord-293966-5c466xvz	28	6	BAC	bac	NN
cord-293966-5c466xvz	28	7	for	for	IN
cord-293966-5c466xvz	28	8	a	a	DT
cord-293966-5c466xvz	28	9	CoV	cov	NN
cord-293966-5c466xvz	28	10	,	,	,
cord-293966-5c466xvz	28	11	transmissible	transmissible	JJ
cord-293966-5c466xvz	28	12	gastroenteritis	gastroenteritis	NN
cord-293966-5c466xvz	28	13	virus	virus	NN
cord-293966-5c466xvz	28	14	(	(	-LRB-
cord-293966-5c466xvz	28	15	TGEV	TGEV	NNP
cord-293966-5c466xvz	28	16	)	)	-RRB-
cord-293966-5c466xvz	28	17	,	,	,
cord-293966-5c466xvz	28	18	was	be	VBD
cord-293966-5c466xvz	28	19	engineered	engineer	VBN
cord-293966-5c466xvz	28	20	,	,	,
cord-293966-5c466xvz	28	21	and	and	CC
cord-293966-5c466xvz	28	22	since	since	IN
cord-293966-5c466xvz	28	23	then	then	RB
cord-293966-5c466xvz	28	24	BACs	bac	NNS
cord-293966-5c466xvz	28	25	have	have	VBP
cord-293966-5c466xvz	28	26	been	be	VBN
cord-293966-5c466xvz	28	27	successfully	successfully	RB
cord-293966-5c466xvz	28	28	developed	develop	VBN
cord-293966-5c466xvz	28	29	for	for	IN
cord-293966-5c466xvz	28	30	several	several	JJ
cord-293966-5c466xvz	28	31	CoVs	CoVs	NNP
cord-293966-5c466xvz	28	32	including	include	VBG
cord-293966-5c466xvz	28	33	feline	feline	JJ
cord-293966-5c466xvz	28	34	infectious	infectious	JJ
cord-293966-5c466xvz	28	35	peritonitis	peritonitis	NN
cord-293966-5c466xvz	28	36	virus	virus	NN
cord-293966-5c466xvz	28	37	(	(	-LRB-
cord-293966-5c466xvz	28	38	FIPV	FIPV	NNP
cord-293966-5c466xvz	28	39	)	)	-RRB-
cord-293966-5c466xvz	28	40	,	,	,
cord-293966-5c466xvz	28	41	OC-43	OC-43	NNP
cord-293966-5c466xvz	28	42	,	,	,
cord-293966-5c466xvz	28	43	SARS	SARS	NNP
cord-293966-5c466xvz	28	44	-	-	HYPH
cord-293966-5c466xvz	28	45	CoV	CoV	NNP
cord-293966-5c466xvz	28	46	,	,	,
cord-293966-5c466xvz	28	47	MERS	MERS	NNP
cord-293966-5c466xvz	28	48	-	-	HYPH
cord-293966-5c466xvz	28	49	CoV	CoV	NNP
cord-293966-5c466xvz	28	50	,	,	,
cord-293966-5c466xvz	29	1	murine	murine	JJ
cord-293966-5c466xvz	29	2	hepatitis	hepatitis	NNP
cord-293966-5c466xvz	29	3	virus	virus	NN
cord-293966-5c466xvz	29	4	strain	strain	NN
cord-293966-5c466xvz	29	5	JHM	JHM	NNP
cord-293966-5c466xvz	30	1	(	(	-LRB-
cord-293966-5c466xvz	30	2	MHV	MHV	NNP
cord-293966-5c466xvz	30	3	-	-	HYPH
cord-293966-5c466xvz	30	4	JHM	JHM	NNP
cord-293966-5c466xvz	30	5	)	)	-RRB-
cord-293966-5c466xvz	30	6	,	,	,
cord-293966-5c466xvz	30	7	porcine	porcine	JJ
cord-293966-5c466xvz	30	8	epidemic	epidemic	NN
cord-293966-5c466xvz	30	9	diarrhea	diarrhea	NN
cord-293966-5c466xvz	30	10	virus	virus	NN
cord-293966-5c466xvz	30	11	(	(	-LRB-
cord-293966-5c466xvz	30	12	PEDV	PEDV	NNP
cord-293966-5c466xvz	30	13	)	)	-RRB-
cord-293966-5c466xvz	30	14	,	,	,
cord-293966-5c466xvz	30	15	and	and	CC
cord-293966-5c466xvz	30	16	the	the	DT
cord-293966-5c466xvz	30	17	SARS	SARS	NNP
cord-293966-5c466xvz	30	18	-	-	HYPH
cord-293966-5c466xvz	30	19	like	like	JJ
cord-293966-5c466xvz	30	20	CoV	CoV	NNP
cord-293966-5c466xvz	30	21	WIV-1	WIV-1	NNP
cord-293966-5c466xvz	31	1	[	[	-LRB-
cord-293966-5c466xvz	31	2	2	2	CD
cord-293966-5c466xvz	31	3	,	,	,
cord-293966-5c466xvz	31	4	4	4	CD
cord-293966-5c466xvz	31	5	,	,	,
cord-293966-5c466xvz	31	6	[	[	-LRB-
cord-293966-5c466xvz	31	7	7	7	CD
cord-293966-5c466xvz	31	8	]	]	-RRB-
cord-293966-5c466xvz	31	9	[	[	-LRB-
cord-293966-5c466xvz	31	10	8	8	CD
cord-293966-5c466xvz	31	11	]	]	-RRB-
cord-293966-5c466xvz	31	12	[	[	-LRB-
cord-293966-5c466xvz	31	13	9	9	CD
cord-293966-5c466xvz	31	14	]	]	-RRB-
cord-293966-5c466xvz	31	15	[	[	-LRB-
cord-293966-5c466xvz	31	16	10	10	CD
cord-293966-5c466xvz	31	17	]	]	-RRB-
cord-293966-5c466xvz	31	18	[	[	-LRB-
cord-293966-5c466xvz	31	19	11	11	CD
cord-293966-5c466xvz	31	20	]	]	-RRB-
cord-293966-5c466xvz	31	21	[	[	-LRB-
cord-293966-5c466xvz	31	22	12	12	CD
cord-293966-5c466xvz	31	23	]	]	-RRB-
cord-293966-5c466xvz	31	24	.	.	.
cord-293966-5c466xvz	32	1	Thus	thus	RB
cord-293966-5c466xvz	32	2	,	,	,
cord-293966-5c466xvz	32	3	it	-PRON-	PRP
cord-293966-5c466xvz	32	4	is	be	VBZ
cord-293966-5c466xvz	32	5	likely	likely	JJ
cord-293966-5c466xvz	32	6	that	that	IN
cord-293966-5c466xvz	32	7	BAC	BAC	NNP
cord-293966-5c466xvz	32	8	-	-	HYPH
cord-293966-5c466xvz	32	9	based	base	VBN
cord-293966-5c466xvz	32	10	reverse	reverse	NN
cord-293966-5c466xvz	32	11	genetics	genetic	NNS
cord-293966-5c466xvz	32	12	could	could	MD
cord-293966-5c466xvz	32	13	be	be	VB
cord-293966-5c466xvz	32	14	useful	useful	JJ
cord-293966-5c466xvz	32	15	for	for	IN
cord-293966-5c466xvz	32	16	any	any	DT
cord-293966-5c466xvz	32	17	novel	novel	JJ
cord-293966-5c466xvz	32	18	or	or	CC
cord-293966-5c466xvz	32	19	emergent	emergent	JJ
cord-293966-5c466xvz	32	20	CoV.	cov.	NN
cord-293966-5c466xvz	33	1	Lambda	lambda	NN
cord-293966-5c466xvz	33	2	red	red	JJ
cord-293966-5c466xvz	33	3	recombination	recombination	NN
cord-293966-5c466xvz	33	4	utilizes	utilize	VBZ
cord-293966-5c466xvz	33	5	bacteriophage	bacteriophage	NN
cord-293966-5c466xvz	33	6	enzymes	enzyme	VBZ
cord-293966-5c466xvz	33	7	Exo	Exo	NNP
cord-293966-5c466xvz	33	8	,	,	,
cord-293966-5c466xvz	33	9	Beta	Beta	NNP
cord-293966-5c466xvz	33	10	,	,	,
cord-293966-5c466xvz	33	11	and	and	CC
cord-293966-5c466xvz	33	12	Gam	Gam	NNP
cord-293966-5c466xvz	33	13	(	(	-LRB-
cord-293966-5c466xvz	33	14	Red	red	JJ
cord-293966-5c466xvz	33	15	proteins	protein	NNS
cord-293966-5c466xvz	33	16	)	)	-RRB-
cord-293966-5c466xvz	33	17	to	to	TO
cord-293966-5c466xvz	33	18	mediate	mediate	VB
cord-293966-5c466xvz	33	19	homologous	homologous	JJ
cord-293966-5c466xvz	33	20	recombination	recombination	NN
cord-293966-5c466xvz	33	21	near	near	IN
cord-293966-5c466xvz	33	22	the	the	DT
cord-293966-5c466xvz	33	23	ends	end	NNS
cord-293966-5c466xvz	33	24	of	of	IN
cord-293966-5c466xvz	33	25	linear	linear	JJ
cord-293966-5c466xvz	33	26	double	double	RB
cord-293966-5c466xvz	33	27	-	-	HYPH
cord-293966-5c466xvz	33	28	stranded	strand	VBN
cord-293966-5c466xvz	33	29	DNA	DNA	NNP
cord-293966-5c466xvz	33	30	[	[	-LRB-
cord-293966-5c466xvz	33	31	13	13	CD
cord-293966-5c466xvz	33	32	,	,	,
cord-293966-5c466xvz	33	33	14	14	CD
cord-293966-5c466xvz	33	34	]	]	-RRB-
cord-293966-5c466xvz	33	35	.	.	.
cord-293966-5c466xvz	34	1	PCR	PCR	NNP
cord-293966-5c466xvz	34	2	products	product	NNS
cord-293966-5c466xvz	34	3	containing	contain	VBG
cord-293966-5c466xvz	34	4	positive	positive	JJ
cord-293966-5c466xvz	34	5	selection	selection	NN
cord-293966-5c466xvz	34	6	markers	marker	NNS
cord-293966-5c466xvz	34	7	are	be	VBP
cord-293966-5c466xvz	34	8	suitable	suitable	JJ
cord-293966-5c466xvz	34	9	substrates	substrate	NNS
cord-293966-5c466xvz	34	10	for	for	IN
cord-293966-5c466xvz	34	11	these	these	DT
cord-293966-5c466xvz	34	12	enzymes	enzyme	NNS
cord-293966-5c466xvz	34	13	,	,	,
cord-293966-5c466xvz	34	14	so	so	RB
cord-293966-5c466xvz	34	15	long	long	RB
cord-293966-5c466xvz	34	16	as	as	IN
cord-293966-5c466xvz	34	17	they	-PRON-	PRP
cord-293966-5c466xvz	34	18	bear	bear	VBP
cord-293966-5c466xvz	34	19	extensions	extension	NNS
cord-293966-5c466xvz	34	20	of	of	IN
cord-293966-5c466xvz	34	21	40	40	CD
cord-293966-5c466xvz	34	22	-	-	SYM
cord-293966-5c466xvz	34	23	50	50	CD
cord-293966-5c466xvz	34	24	bases	basis	NNS
cord-293966-5c466xvz	34	25	that	that	WDT
cord-293966-5c466xvz	34	26	are	be	VBP
cord-293966-5c466xvz	34	27	homologous	homologous	JJ
cord-293966-5c466xvz	34	28	to	to	IN
cord-293966-5c466xvz	34	29	the	the	DT
cord-293966-5c466xvz	34	30	target	target	NN
cord-293966-5c466xvz	34	31	sequence	sequence	NN
cord-293966-5c466xvz	34	32	.	.	.
cord-293966-5c466xvz	35	1	A	a	DT
cord-293966-5c466xvz	35	2	major	major	JJ
cord-293966-5c466xvz	35	3	advancement	advancement	NN
cord-293966-5c466xvz	35	4	in	in	IN
cord-293966-5c466xvz	35	5	this	this	DT
cord-293966-5c466xvz	35	6	technique	technique	NN
cord-293966-5c466xvz	35	7	came	come	VBD
cord-293966-5c466xvz	35	8	with	with	IN
cord-293966-5c466xvz	35	9	the	the	DT
cord-293966-5c466xvz	35	10	development	development	NN
cord-293966-5c466xvz	35	11	of	of	IN
cord-293966-5c466xvz	35	12	an	an	DT
cord-293966-5c466xvz	35	13	E.	E.	NNP
cord-293966-5c466xvz	35	14	coli	coli	NNS
cord-293966-5c466xvz	35	15	strain	strain	NN
cord-293966-5c466xvz	35	16	,	,	,
cord-293966-5c466xvz	35	17	DY380	DY380	NNP
cord-293966-5c466xvz	35	18	,	,	,
cord-293966-5c466xvz	35	19	where	where	WRB
cord-293966-5c466xvz	35	20	the	the	DT
cord-293966-5c466xvz	35	21	Red	red	JJ
cord-293966-5c466xvz	35	22	proteins	protein	NNS
cord-293966-5c466xvz	35	23	were	be	VBD
cord-293966-5c466xvz	35	24	placed	place	VBN
cord-293966-5c466xvz	35	25	under	under	IN
cord-293966-5c466xvz	35	26	the	the	DT
cord-293966-5c466xvz	35	27	control	control	NN
cord-293966-5c466xvz	35	28	of	of	IN
cord-293966-5c466xvz	35	29	a	a	DT
cord-293966-5c466xvz	35	30	temperature	temperature	NN
cord-293966-5c466xvz	35	31	-	-	HYPH
cord-293966-5c466xvz	35	32	inducible	inducible	JJ
cord-293966-5c466xvz	35	33	promoter	promoter	NN
cord-293966-5c466xvz	35	34	[	[	-LRB-
cord-293966-5c466xvz	35	35	15	15	CD
cord-293966-5c466xvz	35	36	]	]	-RRB-
cord-293966-5c466xvz	35	37	.	.	.
cord-293966-5c466xvz	36	1	Several	several	JJ
cord-293966-5c466xvz	36	2	methods	method	NNS
cord-293966-5c466xvz	36	3	for	for	IN
cord-293966-5c466xvz	36	4	removing	remove	VBG
cord-293966-5c466xvz	36	5	the	the	DT
cord-293966-5c466xvz	36	6	positive	positive	JJ
cord-293966-5c466xvz	36	7	selection	selection	NN
cord-293966-5c466xvz	36	8	markers	marker	NNS
cord-293966-5c466xvz	36	9	from	from	IN
cord-293966-5c466xvz	36	10	the	the	DT
cord-293966-5c466xvz	36	11	viral	viral	JJ
cord-293966-5c466xvz	36	12	genomes	genome	NNS
cord-293966-5c466xvz	36	13	have	have	VBP
cord-293966-5c466xvz	36	14	been	be	VBN
cord-293966-5c466xvz	36	15	developed	develop	VBN
cord-293966-5c466xvz	36	16	,	,	,
cord-293966-5c466xvz	36	17	including	include	VBG
cord-293966-5c466xvz	36	18	flanking	flank	VBG
cord-293966-5c466xvz	36	19	sequences	sequence	NNS
cord-293966-5c466xvz	36	20	with	with	IN
cord-293966-5c466xvz	36	21	FRT	FRT	NNP
cord-293966-5c466xvz	36	22	or	or	CC
cord-293966-5c466xvz	36	23	loxP	loxP	NNP
cord-293966-5c466xvz	36	24	sites	site	NNS
cord-293966-5c466xvz	36	25	[	[	-LRB-
cord-293966-5c466xvz	36	26	16	16	CD
cord-293966-5c466xvz	36	27	]	]	-RRB-
cord-293966-5c466xvz	36	28	,	,	,
cord-293966-5c466xvz	36	29	or	or	CC
cord-293966-5c466xvz	36	30	utilizing	utilize	VBG
cord-293966-5c466xvz	36	31	positive	positive	JJ
cord-293966-5c466xvz	36	32	and	and	CC
cord-293966-5c466xvz	36	33	negative	negative	JJ
cord-293966-5c466xvz	36	34	selection	selection	NN
cord-293966-5c466xvz	36	35	markers	marker	NNS
cord-293966-5c466xvz	36	36	on	on	IN
cord-293966-5c466xvz	36	37	a	a	DT
cord-293966-5c466xvz	36	38	single	single	JJ
cord-293966-5c466xvz	36	39	gene	gene	NN
cord-293966-5c466xvz	36	40	cassette	cassette	NN
cord-293966-5c466xvz	36	41	,	,	,
cord-293966-5c466xvz	36	42	such	such	JJ
cord-293966-5c466xvz	36	43	as	as	IN
cord-293966-5c466xvz	36	44	the	the	DT
cord-293966-5c466xvz	36	45	Galactose	Galactose	NNP
cord-293966-5c466xvz	36	46	Kinase	Kinase	NNP
cord-293966-5c466xvz	36	47	(	(	-LRB-
cord-293966-5c466xvz	36	48	GalK)-Kan	GalK)-Kan	NNP
cord-293966-5c466xvz	36	49	R	r	NN
cord-293966-5c466xvz	36	50	gene	gene	NN
cord-293966-5c466xvz	36	51	cassette	cassette	NN
cord-293966-5c466xvz	36	52	[	[	-LRB-
cord-293966-5c466xvz	36	53	17	17	CD
cord-293966-5c466xvz	36	54	]	]	-RRB-
cord-293966-5c466xvz	36	55	.	.	.
cord-293966-5c466xvz	37	1	These	these	DT
cord-293966-5c466xvz	37	2	methods	method	NNS
cord-293966-5c466xvz	37	3	both	both	DT
cord-293966-5c466xvz	37	4	have	have	VBP
cord-293966-5c466xvz	37	5	certain	certain	JJ
cord-293966-5c466xvz	37	6	downfalls	downfall	NNS
cord-293966-5c466xvz	37	7	,	,	,
cord-293966-5c466xvz	37	8	including	include	VBG
cord-293966-5c466xvz	37	9	the	the	DT
cord-293966-5c466xvz	37	10	retention	retention	NN
cord-293966-5c466xvz	37	11	of	of	IN
cord-293966-5c466xvz	37	12	small	small	JJ
cord-293966-5c466xvz	37	13	FRT	FRT	NNP
cord-293966-5c466xvz	37	14	or	or	CC
cord-293966-5c466xvz	37	15	loxP	loxP	NNP
cord-293966-5c466xvz	37	16	sites	site	NNS
cord-293966-5c466xvz	37	17	following	follow	VBG
cord-293966-5c466xvz	37	18	removal	removal	NN
cord-293966-5c466xvz	37	19	of	of	IN
cord-293966-5c466xvz	37	20	the	the	DT
cord-293966-5c466xvz	37	21	marker	marker	NN
cord-293966-5c466xvz	37	22	,	,	,
cord-293966-5c466xvz	37	23	or	or	CC
cord-293966-5c466xvz	37	24	the	the	DT
cord-293966-5c466xvz	37	25	unintended	unintended	JJ
cord-293966-5c466xvz	37	26	removal	removal	NN
cord-293966-5c466xvz	37	27	of	of	IN
cord-293966-5c466xvz	37	28	negative	negative	JJ
cord-293966-5c466xvz	37	29	selection	selection	NN
cord-293966-5c466xvz	37	30	markers	marker	NNS
cord-293966-5c466xvz	37	31	by	by	IN
cord-293966-5c466xvz	37	32	repeat	repeat	NN
cord-293966-5c466xvz	37	33	sequences	sequence	NNS
cord-293966-5c466xvz	37	34	in	in	IN
cord-293966-5c466xvz	37	35	the	the	DT
cord-293966-5c466xvz	37	36	BAC	BAC	NNP
cord-293966-5c466xvz	37	37	plasmid	plasmid	NN
cord-293966-5c466xvz	37	38	.	.	.
cord-293966-5c466xvz	38	1	To	to	TO
cord-293966-5c466xvz	38	2	improve	improve	VB
cord-293966-5c466xvz	38	3	the	the	DT
cord-293966-5c466xvz	38	4	efficiency	efficiency	NN
cord-293966-5c466xvz	38	5	of	of	IN
cord-293966-5c466xvz	38	6	removing	remove	VBG
cord-293966-5c466xvz	38	7	the	the	DT
cord-293966-5c466xvz	38	8	positive	positive	JJ
cord-293966-5c466xvz	38	9	selection	selection	NN
cord-293966-5c466xvz	38	10	marker	marker	NN
cord-293966-5c466xvz	38	11	,	,	,
cord-293966-5c466xvz	38	12	a	a	DT
cord-293966-5c466xvz	38	13	unique	unique	JJ
cord-293966-5c466xvz	38	14	method	method	NN
cord-293966-5c466xvz	38	15	utilizing	utilize	VBG
cord-293966-5c466xvz	38	16	the	the	DT
cord-293966-5c466xvz	38	17	I	I	NNP
cord-293966-5c466xvz	38	18	-	-	HYPH
cord-293966-5c466xvz	38	19	SceI	SceI	NNP
cord-293966-5c466xvz	38	20	homing	homing	NN
cord-293966-5c466xvz	38	21	endonuclease	endonuclease	NN
cord-293966-5c466xvz	38	22	under	under	IN
cord-293966-5c466xvz	38	23	an	an	DT
cord-293966-5c466xvz	38	24	arabinose	arabinose	JJ
cord-293966-5c466xvz	38	25	-	-	HYPH
cord-293966-5c466xvz	38	26	inducible	inducible	JJ
cord-293966-5c466xvz	38	27	promoter	promoter	NN
cord-293966-5c466xvz	38	28	was	be	VBD
cord-293966-5c466xvz	38	29	developed	develop	VBN
cord-293966-5c466xvz	38	30	(	(	-LRB-
cord-293966-5c466xvz	38	31	Fig	Fig	NNP
cord-293966-5c466xvz	38	32	.	.	.
cord-293966-5c466xvz	38	33	1	1	CD
cord-293966-5c466xvz	38	34	)	)	-RRB-
cord-293966-5c466xvz	39	1	[	[	-LRB-
cord-293966-5c466xvz	39	2	18	18	CD
cord-293966-5c466xvz	39	3	]	]	-RRB-
cord-293966-5c466xvz	39	4	.	.	.
cord-293966-5c466xvz	40	1	I	-PRON-	PRP
cord-293966-5c466xvz	40	2	-	-	HYPH
cord-293966-5c466xvz	40	3	SceI	SceI	NNP
cord-293966-5c466xvz	40	4	is	be	VBZ
cord-293966-5c466xvz	40	5	an	an	DT
cord-293966-5c466xvz	40	6	endonuclease	endonuclease	NN
cord-293966-5c466xvz	40	7	with	with	IN
cord-293966-5c466xvz	40	8	an	an	DT
cord-293966-5c466xvz	40	9	18	18	CD
cord-293966-5c466xvz	40	10	bp	bp	NNP
cord-293966-5c466xvz	40	11	recognition	recognition	NN
cord-293966-5c466xvz	40	12	site	site	NN
cord-293966-5c466xvz	40	13	that	that	WDT
cord-293966-5c466xvz	40	14	is	be	VBZ
cord-293966-5c466xvz	40	15	not	not	RB
cord-293966-5c466xvz	40	16	present	present	JJ
cord-293966-5c466xvz	40	17	in	in	IN
cord-293966-5c466xvz	40	18	the	the	DT
cord-293966-5c466xvz	40	19	E.	E.	NNP
cord-293966-5c466xvz	40	20	coli	coli	NNP
cord-293966-5c466xvz	40	21	genome	genome	NN
cord-293966-5c466xvz	40	22	,	,	,
cord-293966-5c466xvz	40	23	making	make	VBG
cord-293966-5c466xvz	40	24	it	-PRON-	PRP
cord-293966-5c466xvz	40	25	safe	safe	JJ
cord-293966-5c466xvz	40	26	to	to	TO
cord-293966-5c466xvz	40	27	express	express	VB
cord-293966-5c466xvz	40	28	in	in	IN
cord-293966-5c466xvz	40	29	E.	E.	NNP
cord-293966-5c466xvz	40	30	coli	coli	NNS
cord-293966-5c466xvz	40	31	.	.	.
cord-293966-5c466xvz	41	1	In	in	IN
cord-293966-5c466xvz	41	2	the	the	DT
cord-293966-5c466xvz	41	3	method	method	NN
cord-293966-5c466xvz	41	4	described	describe	VBN
cord-293966-5c466xvz	41	5	here	here	RB
cord-293966-5c466xvz	41	6	,	,	,
cord-293966-5c466xvz	41	7	this	this	DT
cord-293966-5c466xvz	41	8	recognition	recognition	NN
cord-293966-5c466xvz	41	9	site	site	NN
cord-293966-5c466xvz	41	10	is	be	VBZ
cord-293966-5c466xvz	41	11	engineered	engineer	VBN
cord-293966-5c466xvz	41	12	on	on	IN
cord-293966-5c466xvz	41	13	a	a	DT
cord-293966-5c466xvz	41	14	plasmid	plasmid	NN
cord-293966-5c466xvz	41	15	(	(	-LRB-
cord-293966-5c466xvz	41	16	pEP	pEP	NNP
cord-293966-5c466xvz	41	17	-	-	HYPH
cord-293966-5c466xvz	41	18	KanS	KanS	NNP
cord-293966-5c466xvz	41	19	)	)	-RRB-
cord-293966-5c466xvz	41	20	just	just	RB
cord-293966-5c466xvz	41	21	outside	outside	RB
cord-293966-5c466xvz	41	22	of	of	IN
cord-293966-5c466xvz	41	23	the	the	DT
cord-293966-5c466xvz	41	24	positive	positive	JJ
cord-293966-5c466xvz	41	25	selection	selection	NN
cord-293966-5c466xvz	41	26	marker	marker	NN
cord-293966-5c466xvz	41	27	,	,	,
cord-293966-5c466xvz	41	28	and	and	CC
cord-293966-5c466xvz	41	29	its	-PRON-	PRP$
cord-293966-5c466xvz	41	30	cleavage	cleavage	NN
cord-293966-5c466xvz	41	31	with	with	IN
cord-293966-5c466xvz	41	32	the	the	DT
cord-293966-5c466xvz	41	33	I	I	NNP
cord-293966-5c466xvz	41	34	-	-	HYPH
cord-293966-5c466xvz	41	35	SceI	SceI	NNP
cord-293966-5c466xvz	41	36	enzyme	enzyme	NN
cord-293966-5c466xvz	41	37	allows	allow	VBZ
cord-293966-5c466xvz	41	38	for	for	IN
cord-293966-5c466xvz	41	39	the	the	DT
cord-293966-5c466xvz	41	40	removal	removal	NN
cord-293966-5c466xvz	41	41	of	of	IN
cord-293966-5c466xvz	41	42	the	the	DT
cord-293966-5c466xvz	41	43	positive	positive	JJ
cord-293966-5c466xvz	41	44	selection	selection	NN
cord-293966-5c466xvz	41	45	marker	marker	NN
cord-293966-5c466xvz	41	46	by	by	IN
cord-293966-5c466xvz	41	47	intramolecular	intramolecular	JJ
cord-293966-5c466xvz	41	48	Red	red	JJ
cord-293966-5c466xvz	41	49	recombination	recombination	NN
cord-293966-5c466xvz	41	50	utilizing	utilize	VBG
cord-293966-5c466xvz	41	51	sequence	sequence	NN
cord-293966-5c466xvz	41	52	duplication	duplication	NN
cord-293966-5c466xvz	41	53	introduced	introduce	VBN
cord-293966-5c466xvz	41	54	in	in	IN
cord-293966-5c466xvz	41	55	the	the	DT
cord-293966-5c466xvz	41	56	original	original	JJ
cord-293966-5c466xvz	41	57	PCR	PCR	NNP
cord-293966-5c466xvz	41	58	primers	primer	NNS
cord-293966-5c466xvz	41	59	.	.	.
cord-293966-5c466xvz	42	1	This	this	DT
cord-293966-5c466xvz	42	2	method	method	NN
cord-293966-5c466xvz	42	3	can	can	MD
cord-293966-5c466xvz	42	4	be	be	VB
cord-293966-5c466xvz	42	5	utilized	utilize	VBN
cord-293966-5c466xvz	42	6	to	to	TO
cord-293966-5c466xvz	42	7	introduce	introduce	VB
cord-293966-5c466xvz	42	8	any	any	DT
cord-293966-5c466xvz	42	9	type	type	NN
cord-293966-5c466xvz	42	10	of	of	IN
cord-293966-5c466xvz	42	11	modification	modification	NN
cord-293966-5c466xvz	42	12	into	into	IN
cord-293966-5c466xvz	42	13	the	the	DT
cord-293966-5c466xvz	42	14	BAC	BAC	NNP
cord-293966-5c466xvz	42	15	DNA	DNA	NNP
cord-293966-5c466xvz	42	16	,	,	,
cord-293966-5c466xvz	42	17	including	include	VBG
cord-293966-5c466xvz	42	18	mutations	mutation	NNS
cord-293966-5c466xvz	42	19	,	,	,
cord-293966-5c466xvz	42	20	deletions	deletion	NNS
cord-293966-5c466xvz	42	21	,	,	,
cord-293966-5c466xvz	42	22	and	and	CC
cord-293966-5c466xvz	42	23	insertions	insertion	NNS
cord-293966-5c466xvz	42	24	.	.	.
cord-293966-5c466xvz	43	1	Here	here	RB
cord-293966-5c466xvz	43	2	I	-PRON-	PRP
cord-293966-5c466xvz	43	3	will	will	MD
cord-293966-5c466xvz	43	4	outline	outline	VB
cord-293966-5c466xvz	43	5	the	the	DT
cord-293966-5c466xvz	43	6	procedure	procedure	NN
cord-293966-5c466xvz	43	7	for	for	IN
cord-293966-5c466xvz	43	8	this	this	DT
cord-293966-5c466xvz	43	9	highly	highly	RB
cord-293966-5c466xvz	43	10	efficient	efficient	JJ
cord-293966-5c466xvz	43	11	method	method	NN
cord-293966-5c466xvz	43	12	to	to	TO
cord-293966-5c466xvz	43	13	engineer	engineer	VB
cord-293966-5c466xvz	43	14	markerless	markerless	NN
cord-293966-5c466xvz	43	15	modifications	modification	NNS
cord-293966-5c466xvz	43	16	,	,	,
cord-293966-5c466xvz	43	17	focusing	focus	VBG
cord-293966-5c466xvz	43	18	on	on	IN
cord-293966-5c466xvz	43	19	single	single	JJ
cord-293966-5c466xvz	43	20	point	point	NN
cord-293966-5c466xvz	43	21	mutations	mutation	NNS
cord-293966-5c466xvz	43	22	in	in	IN
cord-293966-5c466xvz	43	23	the	the	DT
cord-293966-5c466xvz	43	24	full	full	JJ
cord-293966-5c466xvz	43	25	-	-	HYPH
cord-293966-5c466xvz	43	26	length	length	NN
cord-293966-5c466xvz	43	27	MERS	MERS	NNP
cord-293966-5c466xvz	43	28	-	-	HYPH
cord-293966-5c466xvz	43	29	CoV	CoV	NNP
cord-293966-5c466xvz	43	30	BAC	BAC	NNP
cord-293966-5c466xvz	43	31	.	.	.
cord-293966-5c466xvz	44	1	[	[	-LRB-
cord-293966-5c466xvz	44	2	2	2	CD
cord-293966-5c466xvz	44	3	]	]	-RRB-
cord-293966-5c466xvz	44	4	.	.	.
cord-293966-5c466xvz	45	1	The	the	DT
cord-293966-5c466xvz	45	2	full	full	JJ
cord-293966-5c466xvz	45	3	protocol	protocol	NN
cord-293966-5c466xvz	45	4	for	for	IN
cord-293966-5c466xvz	45	5	creating	create	VBG
cord-293966-5c466xvz	45	6	this	this	DT
cord-293966-5c466xvz	45	7	BAC	BAC	NNP
cord-293966-5c466xvz	45	8	was	be	VBD
cord-293966-5c466xvz	45	9	subsequently	subsequently	RB
cord-293966-5c466xvz	45	10	published	publish	VBN
cord-293966-5c466xvz	45	11	by	by	IN
cord-293966-5c466xvz	45	12	the	the	DT
cord-293966-5c466xvz	45	13	same	same	JJ
cord-293966-5c466xvz	45	14	group	group	NN
cord-293966-5c466xvz	45	15	in	in	IN
cord-293966-5c466xvz	45	16	a	a	DT
cord-293966-5c466xvz	45	17	previous	previous	JJ
cord-293966-5c466xvz	45	18	Methods	method	NNS
cord-293966-5c466xvz	45	19	in	in	IN
cord-293966-5c466xvz	45	20	Molecular	Molecular	NNP
cord-293966-5c466xvz	45	21	Biology	Biology	NNP
cord-293966-5c466xvz	45	22	book	book	NN
cord-293966-5c466xvz	45	23	[	[	-LRB-
cord-293966-5c466xvz	45	24	19	19	CD
cord-293966-5c466xvz	45	25	]	]	-RRB-
cord-293966-5c466xvz	45	26	.	.	.
cord-293966-5c466xvz	46	1	This	this	DT
cord-293966-5c466xvz	46	2	plasmid	plasmid	NN
cord-293966-5c466xvz	46	3	contains	contain	VBZ
cord-293966-5c466xvz	46	4	the	the	DT
cord-293966-5c466xvz	46	5	parA	parA	NNP
cord-293966-5c466xvz	46	6	,	,	,
cord-293966-5c466xvz	46	7	parB	parb	CC
cord-293966-5c466xvz	46	8	,	,	,
cord-293966-5c466xvz	46	9	and	and	CC
cord-293966-5c466xvz	46	10	parC	parc	IN
cord-293966-5c466xvz	46	11	genes	gene	NNS
cord-293966-5c466xvz	46	12	derived	derive	VBN
cord-293966-5c466xvz	46	13	from	from	IN
cord-293966-5c466xvz	46	14	the	the	DT
cord-293966-5c466xvz	46	15	E.	E.	NNP
cord-293966-5c466xvz	46	16	coli	coli	VBZ
cord-293966-5c466xvz	46	17	F	F	NNP
cord-293966-5c466xvz	46	18	-	-	HYPH
cord-293966-5c466xvz	46	19	factor	factor	NN
cord-293966-5c466xvz	46	20	to	to	TO
cord-293966-5c466xvz	46	21	prevent	prevent	VB
cord-293966-5c466xvz	46	22	more	more	JJR
cord-293966-5c466xvz	46	23	than	than	IN
cord-293966-5c466xvz	46	24	one	one	CD
cord-293966-5c466xvz	46	25	or	or	CC
cord-293966-5c466xvz	46	26	two	two	CD
cord-293966-5c466xvz	46	27	BACs	bac	NNS
cord-293966-5c466xvz	46	28	from	from	IN
cord-293966-5c466xvz	46	29	coexisting	coexist	VBG
cord-293966-5c466xvz	46	30	in	in	IN
cord-293966-5c466xvz	46	31	the	the	DT
cord-293966-5c466xvz	46	32	same	same	JJ
cord-293966-5c466xvz	46	33	cell	cell	NN
cord-293966-5c466xvz	46	34	.	.	.
cord-293966-5c466xvz	47	1	It	-PRON-	PRP
cord-293966-5c466xvz	47	2	also	also	RB
cord-293966-5c466xvz	47	3	contains	contain	VBZ
cord-293966-5c466xvz	47	4	genes	gene	NNS
cord-293966-5c466xvz	47	5	involved	involve	VBN
cord-293966-5c466xvz	47	6	in	in	IN
cord-293966-5c466xvz	47	7	the	the	DT
cord-293966-5c466xvz	47	8	initiation	initiation	NN
cord-293966-5c466xvz	47	9	and	and	CC
cord-293966-5c466xvz	47	10	orientation	orientation	NN
cord-293966-5c466xvz	47	11	of	of	IN
cord-293966-5c466xvz	47	12	DNA	dna	NN
cord-293966-5c466xvz	47	13	replication	replication	NN
cord-293966-5c466xvz	47	14	and	and	CC
cord-293966-5c466xvz	47	15	the	the	DT
cord-293966-5c466xvz	47	16	chloramphenicol	chloramphenicol	NN
cord-293966-5c466xvz	47	17	resistance	resistance	NN
cord-293966-5c466xvz	47	18	gene	gene	NN
cord-293966-5c466xvz	47	19	(	(	-LRB-
cord-293966-5c466xvz	47	20	Cml	Cml	NNP
cord-293966-5c466xvz	47	21	r	r	NNP
cord-293966-5c466xvz	47	22	)	)	-RRB-
cord-293966-5c466xvz	47	23	.	.	.
cord-293966-5c466xvz	48	1	2	2	LS
cord-293966-5c466xvz	48	2	.	.	.
cord-293966-5c466xvz	49	1	pEP	pEP	NNP
cord-293966-5c466xvz	49	2	-	-	HYPH
cord-293966-5c466xvz	49	3	KanS.	KanS.	NNP
cord-293966-5c466xvz	50	1	This	this	DT
cord-293966-5c466xvz	50	2	plasmid	plasmid	NN
cord-293966-5c466xvz	50	3	contains	contain	VBZ
cord-293966-5c466xvz	50	4	the	the	DT
cord-293966-5c466xvz	50	5	AphAI	aphai	NN
cord-293966-5c466xvz	50	6	-	-	HYPH
cord-293966-5c466xvz	50	7	I	I	NNP
cord-293966-5c466xvz	50	8	-	-	HYPH
cord-293966-5c466xvz	50	9	SceI	SceI	NNP
cord-293966-5c466xvz	50	10	cassette	cassette	NN
cord-293966-5c466xvz	50	11	containing	contain	VBG
cord-293966-5c466xvz	50	12	a	a	DT
cord-293966-5c466xvz	50	13	kanamycin	kanamycin	NNP
cord-293966-5c466xvz	50	14	resistance	resistance	NN
cord-293966-5c466xvz	50	15	marker	marker	NN
cord-293966-5c466xvz	50	16	(	(	-LRB-
cord-293966-5c466xvz	50	17	Kan	Kan	NNP
cord-293966-5c466xvz	50	18	r	r	NNP
cord-293966-5c466xvz	50	19	)	)	-RRB-
cord-293966-5c466xvz	50	20	and	and	CC
cord-293966-5c466xvz	50	21	an	an	DT
cord-293966-5c466xvz	50	22	I	I	NNP
cord-293966-5c466xvz	50	23	-	-	HYPH
cord-293966-5c466xvz	50	24	SceI	SceI	NNP
cord-293966-5c466xvz	50	25	restriction	restriction	NN
cord-293966-5c466xvz	50	26	site	site	NN
cord-293966-5c466xvz	50	27	[	[	-LRB-
cord-293966-5c466xvz	50	28	18	18	CD
cord-293966-5c466xvz	50	29	]	]	-RRB-
cord-293966-5c466xvz	50	30	.	.	.
cord-293966-5c466xvz	51	1	This	this	DT
cord-293966-5c466xvz	51	2	plasmid	plasmid	NN
cord-293966-5c466xvz	51	3	also	also	RB
cord-293966-5c466xvz	51	4	contains	contain	VBZ
cord-293966-5c466xvz	51	5	an	an	DT
cord-293966-5c466xvz	51	6	ampicillin	ampicillin	JJ
cord-293966-5c466xvz	51	7	resistance	resistance	NN
cord-293966-5c466xvz	51	8	marker	marker	NN
cord-293966-5c466xvz	51	9	.	.	.
cord-293966-5c466xvz	52	1	3	3	LS
cord-293966-5c466xvz	52	2	.	.	.
cord-293966-5c466xvz	53	1	E.	E.	NNP
cord-293966-5c466xvz	53	2	coli	coli	VBZ
cord-293966-5c466xvz	53	3	strains	strain	NNS
cord-293966-5c466xvz	53	4	DH10B	dh10b	JJ
cord-293966-5c466xvz	53	5	(	(	-LRB-
cord-293966-5c466xvz	53	6	see	see	VB
cord-293966-5c466xvz	53	7	Note1	Note1	NNP
cord-293966-5c466xvz	53	8	)	)	-RRB-
cord-293966-5c466xvz	53	9	and	and	CC
cord-293966-5c466xvz	53	10	GS1783	GS1783	NNP
cord-293966-5c466xvz	53	11	(	(	-LRB-
cord-293966-5c466xvz	53	12	see	see	VB
cord-293966-5c466xvz	53	13	Note	note	NN
cord-293966-5c466xvz	53	14	2	2	CD
cord-293966-5c466xvz	53	15	)	)	-RRB-
cord-293966-5c466xvz	53	16	cells	cell	NNS
cord-293966-5c466xvz	53	17	.	.	.
cord-293966-5c466xvz	53	18	 	 	_SP
cord-293966-5c466xvz	54	1	Forward	forward	RB
cord-293966-5c466xvz	54	2	6	6	CD
cord-293966-5c466xvz	54	3	.	.	.
cord-293966-5c466xvz	55	1	Aliquot	Aliquot	NNP
cord-293966-5c466xvz	55	2	25	25	CD
cord-293966-5c466xvz	55	3	mL	ml	NN
cord-293966-5c466xvz	55	4	into	into	IN
cord-293966-5c466xvz	55	5	each	each	DT
cord-293966-5c466xvz	55	6	50	50	CD
cord-293966-5c466xvz	55	7	mL	ml	NN
cord-293966-5c466xvz	55	8	conical	conical	JJ
cord-293966-5c466xvz	55	9	tube	tube	NN
cord-293966-5c466xvz	55	10	on	on	IN
cord-293966-5c466xvz	55	11	ice	ice	NN
cord-293966-5c466xvz	55	12	.	.	.
cord-293966-5c466xvz	56	1	Centrifuge	centrifuge	NN
cord-293966-5c466xvz	56	2	at	at	IN
cord-293966-5c466xvz	56	3	1800	1800	CD
cord-293966-5c466xvz	56	4	Â	â	NN
cord-293966-5c466xvz	56	5	g	g	NN
cord-293966-5c466xvz	56	6	for	for	IN
cord-293966-5c466xvz	56	7	10	10	CD
cord-293966-5c466xvz	56	8	min	min	NN
cord-293966-5c466xvz	56	9	at	at	IN
cord-293966-5c466xvz	56	10	4	4	CD
cord-293966-5c466xvz	56	11	C	c	NN
cord-293966-5c466xvz	56	12	in	in	IN
cord-293966-5c466xvz	56	13	a	a	DT
cord-293966-5c466xvz	56	14	tabletop	tabletop	NN
cord-293966-5c466xvz	56	15	centrifuge	centrifuge	NN
cord-293966-5c466xvz	56	16	.	.	.
cord-293966-5c466xvz	57	1	7	7	CD
cord-293966-5c466xvz	57	2	.	.	.
cord-293966-5c466xvz	58	1	Pour	pour	VB
cord-293966-5c466xvz	58	2	off	off	RP
cord-293966-5c466xvz	58	3	supernatant	supernatant	JJ
cord-293966-5c466xvz	58	4	with	with	IN
cord-293966-5c466xvz	58	5	one	one	CD
cord-293966-5c466xvz	58	6	quick	quick	JJ
cord-293966-5c466xvz	58	7	motion	motion	NN
cord-293966-5c466xvz	58	8	.	.	.
cord-293966-5c466xvz	59	1	While	while	IN
cord-293966-5c466xvz	59	2	pouring	pour	VBG
cord-293966-5c466xvz	59	3	,	,	,
cord-293966-5c466xvz	59	4	position	position	VB
cord-293966-5c466xvz	59	5	the	the	DT
cord-293966-5c466xvz	59	6	bacterial	bacterial	JJ
cord-293966-5c466xvz	59	7	pellet	pellet	NN
cord-293966-5c466xvz	59	8	away	away	RB
cord-293966-5c466xvz	59	9	from	from	IN
cord-293966-5c466xvz	59	10	the	the	DT
cord-293966-5c466xvz	59	11	liquid	liquid	NN
cord-293966-5c466xvz	59	12	to	to	TO
cord-293966-5c466xvz	59	13	limit	limit	VB
cord-293966-5c466xvz	59	14	the	the	DT
cord-293966-5c466xvz	59	15	amount	amount	NN
cord-293966-5c466xvz	59	16	of	of	IN
cord-293966-5c466xvz	59	17	bacteria	bacteria	NNS
cord-293966-5c466xvz	59	18	lost	lose	VBN
cord-293966-5c466xvz	59	19	.	.	.
cord-293966-5c466xvz	60	1	Add	add	VB
cord-293966-5c466xvz	60	2	5	5	CD
cord-293966-5c466xvz	60	3	mL	ml	NN
cord-293966-5c466xvz	60	4	ice	ice	NN
cord-293966-5c466xvz	60	5	-	-	HYPH
cord-293966-5c466xvz	60	6	cold	cold	NN
cord-293966-5c466xvz	60	7	sterile	sterile	JJ
cord-293966-5c466xvz	60	8	ddH	ddh	JJ
cord-293966-5c466xvz	60	9	2	2	CD
cord-293966-5c466xvz	60	10	O	o	NN
cord-293966-5c466xvz	60	11	and	and	CC
cord-293966-5c466xvz	60	12	resuspend	resuspend	VB
cord-293966-5c466xvz	60	13	pellet	pellet	NN
cord-293966-5c466xvz	60	14	by	by	IN
cord-293966-5c466xvz	60	15	swirling	swirl	VBG
cord-293966-5c466xvz	60	16	and	and	CC
cord-293966-5c466xvz	60	17	tapping	tap	VBG
cord-293966-5c466xvz	60	18	the	the	DT
cord-293966-5c466xvz	60	19	tube	tube	NN
cord-293966-5c466xvz	60	20	to	to	IN
cord-293966-5c466xvz	60	21	the	the	DT
cord-293966-5c466xvz	60	22	bottom	bottom	NN
cord-293966-5c466xvz	60	23	of	of	IN
cord-293966-5c466xvz	60	24	the	the	DT
cord-293966-5c466xvz	60	25	ice	ice	NN
cord-293966-5c466xvz	60	26	-	-	HYPH
cord-293966-5c466xvz	60	27	cold	cold	JJ
cord-293966-5c466xvz	60	28	autoclave	autoclave	NN
cord-293966-5c466xvz	60	29	bin	bin	NNP
cord-293966-5c466xvz	60	30	.	.	.
cord-293966-5c466xvz	61	1	Once	once	RB
cord-293966-5c466xvz	61	2	resuspended	resuspend	VBN
cord-293966-5c466xvz	61	3	,	,	,
cord-293966-5c466xvz	61	4	add	add	VB
cord-293966-5c466xvz	61	5	an	an	DT
cord-293966-5c466xvz	61	6	additional	additional	JJ
cord-293966-5c466xvz	61	7	20	20	CD
cord-293966-5c466xvz	61	8	mL	ml	NN
cord-293966-5c466xvz	61	9	ice	ice	NN
cord-293966-5c466xvz	61	10	-	-	HYPH
cord-293966-5c466xvz	61	11	cold	cold	NN
cord-293966-5c466xvz	61	12	sterile	sterile	JJ
cord-293966-5c466xvz	61	13	ddH	ddh	JJ
cord-293966-5c466xvz	61	14	2	2	CD
cord-293966-5c466xvz	61	15	O	o	NN
cord-293966-5c466xvz	61	16	and	and	CC
cord-293966-5c466xvz	61	17	centrifuge	centrifuge	NN
cord-293966-5c466xvz	61	18	at	at	IN
cord-293966-5c466xvz	61	19	1800	1800	CD
cord-293966-5c466xvz	61	20	Â	â	NN
cord-293966-5c466xvz	61	21	g	g	NN
cord-293966-5c466xvz	61	22	for	for	IN
cord-293966-5c466xvz	61	23	10	10	CD
cord-293966-5c466xvz	61	24	min	min	NN
cord-293966-5c466xvz	61	25	at	at	IN
cord-293966-5c466xvz	61	26	4	4	CD
cord-293966-5c466xvz	61	27	C.	C.	NNP
cord-293966-5c466xvz	61	28	Repeat	Repeat	NNP
cord-293966-5c466xvz	61	29	1Â.	1â.	CD
cord-293966-5c466xvz	61	30	8	8	CD
cord-293966-5c466xvz	61	31	.	.	.
cord-293966-5c466xvz	62	1	Following	follow	VBG
cord-293966-5c466xvz	62	2	the	the	DT
cord-293966-5c466xvz	62	3	second	second	JJ
cord-293966-5c466xvz	62	4	water	water	NN
cord-293966-5c466xvz	62	5	wash	wash	NN
cord-293966-5c466xvz	62	6	,	,	,
cord-293966-5c466xvz	62	7	resuspend	resuspend	VB
cord-293966-5c466xvz	62	8	each	each	DT
cord-293966-5c466xvz	62	9	pellet	pellet	NN
cord-293966-5c466xvz	62	10	with	with	IN
cord-293966-5c466xvz	62	11	10	10	CD
cord-293966-5c466xvz	62	12	%	%	NN
cord-293966-5c466xvz	62	13	glycerol	glycerol	NN
cord-293966-5c466xvz	62	14	,	,	,
cord-293966-5c466xvz	62	15	first	first	RB
cord-293966-5c466xvz	62	16	in	in	IN
cord-293966-5c466xvz	62	17	5	5	CD
cord-293966-5c466xvz	62	18	mL	ml	NN
cord-293966-5c466xvz	62	19	,	,	,
cord-293966-5c466xvz	62	20	then	then	RB
cord-293966-5c466xvz	62	21	add	add	VB
cord-293966-5c466xvz	62	22	an	an	DT
cord-293966-5c466xvz	62	23	additional	additional	JJ
cord-293966-5c466xvz	62	24	15	15	CD
cord-293966-5c466xvz	62	25	mL.	mL.	NNS
cord-293966-5c466xvz	62	26	Centrifuge	Centrifuge	NNP
cord-293966-5c466xvz	62	27	at	at	IN
cord-293966-5c466xvz	62	28	1800	1800	CD
cord-293966-5c466xvz	62	29	Â	â	NN
cord-293966-5c466xvz	62	30	g	g	NN
cord-293966-5c466xvz	62	31	for	for	IN
cord-293966-5c466xvz	62	32	10	10	CD
cord-293966-5c466xvz	62	33	min	min	NN
cord-293966-5c466xvz	62	34	at	at	IN
cord-293966-5c466xvz	62	35	4	4	CD
cord-293966-5c466xvz	62	36	C.	C.	NNP
cord-293966-5c466xvz	62	37	9	9	CD
cord-293966-5c466xvz	62	38	.	.	.
cord-293966-5c466xvz	63	1	Pour	pour	VB
cord-293966-5c466xvz	63	2	off	off	RP
cord-293966-5c466xvz	63	3	supernatant	supernatant	JJ
cord-293966-5c466xvz	63	4	as	as	IN
cord-293966-5c466xvz	63	5	described	describe	VBN
cord-293966-5c466xvz	63	6	above	above	RB
cord-293966-5c466xvz	63	7	.	.	.
cord-293966-5c466xvz	64	1	Following	follow	VBG
cord-293966-5c466xvz	64	2	the	the	DT
cord-293966-5c466xvz	64	3	pour	pour	NN
cord-293966-5c466xvz	64	4	,	,	,
cord-293966-5c466xvz	64	5	resuspend	resuspend	VB
cord-293966-5c466xvz	64	6	the	the	DT
cord-293966-5c466xvz	64	7	pellet	pellet	NN
cord-293966-5c466xvz	64	8	in	in	IN
cord-293966-5c466xvz	64	9	the	the	DT
cord-293966-5c466xvz	64	10	remaining	remain	VBG
cord-293966-5c466xvz	64	11	10	10	CD
cord-293966-5c466xvz	64	12	%	%	NN
cord-293966-5c466xvz	64	13	glycerol	glycerol	NN
cord-293966-5c466xvz	64	14	(	(	-LRB-
cord-293966-5c466xvz	64	15	~500	~500	NNP
cord-293966-5c466xvz	64	16	μL	μl	ADD
cord-293966-5c466xvz	64	17	)	)	-RRB-
cord-293966-5c466xvz	64	18	.	.	.
cord-293966-5c466xvz	65	1	If	if	IN
cord-293966-5c466xvz	65	2	the	the	DT
cord-293966-5c466xvz	65	3	combined	combined	JJ
cord-293966-5c466xvz	65	4	amount	amount	NN
cord-293966-5c466xvz	65	5	of	of	IN
cord-293966-5c466xvz	65	6	cells	cell	NNS
cord-293966-5c466xvz	65	7	and	and	CC
cord-293966-5c466xvz	65	8	10	10	CD
cord-293966-5c466xvz	65	9	%	%	NN
cord-293966-5c466xvz	65	10	glycerol	glycerol	NN
cord-293966-5c466xvz	65	11	is	be	VBZ
cord-293966-5c466xvz	65	12	greater	great	JJR
cord-293966-5c466xvz	65	13	than	than	IN
cord-293966-5c466xvz	65	14	550	550	CD
cord-293966-5c466xvz	65	15	μL	μL	NNS
cord-293966-5c466xvz	65	16	,	,	,
cord-293966-5c466xvz	65	17	transfer	transfer	VB
cord-293966-5c466xvz	65	18	the	the	DT
cord-293966-5c466xvz	65	19	suspension	suspension	NN
cord-293966-5c466xvz	65	20	to	to	IN
cord-293966-5c466xvz	65	21	a	a	DT
cord-293966-5c466xvz	65	22	cold	cold	JJ
cord-293966-5c466xvz	65	23	Eppendorf	eppendorf	NN
cord-293966-5c466xvz	65	24	tube	tube	NN
cord-293966-5c466xvz	65	25	and	and	CC
cord-293966-5c466xvz	65	26	pellet	pellet	NN
cord-293966-5c466xvz	65	27	the	the	DT
cord-293966-5c466xvz	65	28	cells	cell	NNS
cord-293966-5c466xvz	65	29	for	for	IN
cord-293966-5c466xvz	65	30	2	2	CD
cord-293966-5c466xvz	65	31	min	min	NN
cord-293966-5c466xvz	65	32	at	at	IN
cord-293966-5c466xvz	65	33	5000	5000	CD
cord-293966-5c466xvz	65	34	Â	â	NN
cord-293966-5c466xvz	65	35	g	g	NN
cord-293966-5c466xvz	65	36	and	and	CC
cord-293966-5c466xvz	65	37	4	4	CD
cord-293966-5c466xvz	65	38	C.	C.	NNP
cord-293966-5c466xvz	65	39	After	after	IN
cord-293966-5c466xvz	65	40	spinning	spin	VBG
cord-293966-5c466xvz	65	41	the	the	DT
cord-293966-5c466xvz	65	42	cells	cell	NNS
cord-293966-5c466xvz	65	43	,	,	,
cord-293966-5c466xvz	65	44	remove	remove	VB
cord-293966-5c466xvz	65	45	an	an	DT
cord-293966-5c466xvz	65	46	appropriate	appropriate	JJ
cord-293966-5c466xvz	65	47	amount	amount	NN
cord-293966-5c466xvz	65	48	of	of	IN
cord-293966-5c466xvz	65	49	supernatant	supernatant	JJ
cord-293966-5c466xvz	65	50	such	such	JJ
cord-293966-5c466xvz	65	51	that~500	that~500	NNP
cord-293966-5c466xvz	65	52	μL	μL	NFP
cord-293966-5c466xvz	65	53	of	of	IN
cord-293966-5c466xvz	65	54	cell	cell	NN
cord-293966-5c466xvz	65	55	suspension	suspension	NN
cord-293966-5c466xvz	65	56	remains	remain	VBZ
cord-293966-5c466xvz	65	57	.	.	.
cord-293966-5c466xvz	66	1	Resuspend	resuspend	VB
cord-293966-5c466xvz	66	2	the	the	DT
cord-293966-5c466xvz	66	3	cells	cell	NNS
cord-293966-5c466xvz	66	4	to	to	IN
cord-293966-5c466xvz	66	5	a	a	DT
cord-293966-5c466xvz	66	6	homogenous	homogenous	JJ
cord-293966-5c466xvz	66	7	solution	solution	NN
cord-293966-5c466xvz	66	8	and	and	CC
cord-293966-5c466xvz	66	9	aliquot	aliquot	VBD
cord-293966-5c466xvz	66	10	50	50	CD
cord-293966-5c466xvz	67	1	μL	μL	.
cord-293966-5c466xvz	68	1	to	to	TO
cord-293966-5c466xvz	68	2	prefrozen	prefrozen	VB
cord-293966-5c466xvz	68	3	Eppendorf	Eppendorf	NNP
cord-293966-5c466xvz	68	4	tubes	tube	NNS
cord-293966-5c466xvz	68	5	and	and	CC
cord-293966-5c466xvz	68	6	flash	flash	NN
cord-293966-5c466xvz	68	7	-	-	HYPH
cord-293966-5c466xvz	68	8	freeze	freeze	NN
cord-293966-5c466xvz	68	9	tubes	tube	NNS
cord-293966-5c466xvz	68	10	in	in	IN
cord-293966-5c466xvz	68	11	liquid	liquid	JJ
cord-293966-5c466xvz	68	12	nitrogen	nitrogen	NN
cord-293966-5c466xvz	68	13	or	or	CC
cord-293966-5c466xvz	68	14	a	a	DT
cord-293966-5c466xvz	68	15	dry	dry	JJ
cord-293966-5c466xvz	68	16	ice	ice	NN
cord-293966-5c466xvz	68	17	-	-	HYPH
cord-293966-5c466xvz	68	18	methanol	methanol	NN
cord-293966-5c466xvz	68	19	bath	bath	NN
cord-293966-5c466xvz	68	20	.	.	.
cord-293966-5c466xvz	69	1	Use	use	VB
cord-293966-5c466xvz	69	2	immediately	immediately	RB
cord-293966-5c466xvz	69	3	or	or	CC
cord-293966-5c466xvz	69	4	store	store	VB
cord-293966-5c466xvz	69	5	at	at	IN
cord-293966-5c466xvz	69	6	À80	À80	NNP
cord-293966-5c466xvz	69	7	C.	C.	NNP
cord-293966-5c466xvz	69	8	Cells	Cells	NNP
cord-293966-5c466xvz	69	9	are	be	VBP
cord-293966-5c466xvz	69	10	typically	typically	RB
cord-293966-5c466xvz	69	11	good	good	JJ
cord-293966-5c466xvz	69	12	for	for	IN
cord-293966-5c466xvz	69	13	6	6	CD
cord-293966-5c466xvz	69	14	-	-	SYM
cord-293966-5c466xvz	69	15	12	12	CD
cord-293966-5c466xvz	69	16	months	month	NNS
cord-293966-5c466xvz	69	17	,	,	,
cord-293966-5c466xvz	69	18	but	but	CC
cord-293966-5c466xvz	69	19	may	may	MD
cord-293966-5c466xvz	69	20	be	be	VB
cord-293966-5c466xvz	69	21	useful	useful	JJ
cord-293966-5c466xvz	69	22	even	even	RB
cord-293966-5c466xvz	69	23	after	after	IN
cord-293966-5c466xvz	69	24	several	several	JJ
cord-293966-5c466xvz	69	25	years	year	NNS
cord-293966-5c466xvz	69	26	.	.	.
cord-293966-5c466xvz	69	27	 	 	_SP
cord-293966-5c466xvz	70	1	Kan	Kan	NNP
cord-293966-5c466xvz	70	2	r	r	NNP
cord-293966-5c466xvz	70	3	-I	-I	NNP
cord-293966-5c466xvz	70	4	-	-	HYPH
cord-293966-5c466xvz	70	5	SceI	SceI	NNP
cord-293966-5c466xvz	70	6	primers	primer	NNS
cord-293966-5c466xvz	70	7	with	with	IN
cord-293966-5c466xvz	70	8	60	60	CD
cord-293966-5c466xvz	70	9	bp	bp	NNP
cord-293966-5c466xvz	70	10	homology	homology	NN
cord-293966-5c466xvz	70	11	to	to	IN
cord-293966-5c466xvz	70	12	the	the	DT
cord-293966-5c466xvz	70	13	region	region	NN
cord-293966-5c466xvz	70	14	of	of	IN
cord-293966-5c466xvz	70	15	interest	interest	NN
cord-293966-5c466xvz	70	16	flanking	flank	VBG
cord-293966-5c466xvz	70	17	the	the	DT
cord-293966-5c466xvz	70	18	desired	desire	VBN
cord-293966-5c466xvz	70	19	site	site	NN
cord-293966-5c466xvz	70	20	to	to	TO
cord-293966-5c466xvz	70	21	be	be	VB
cord-293966-5c466xvz	70	22	modified	modify	VBN
cord-293966-5c466xvz	70	23	(	(	-LRB-
cord-293966-5c466xvz	70	24	Fig	Fig	NNP
cord-293966-5c466xvz	70	25	.	.	.
cord-293966-5c466xvz	70	26	1a	1a	CD
cord-293966-5c466xvz	70	27	)	)	-RRB-
cord-293966-5c466xvz	70	28	.	.	.
cord-293966-5c466xvz	71	1	To	to	TO
cord-293966-5c466xvz	71	2	design	design	VB
cord-293966-5c466xvz	71	3	simple	simple	JJ
cord-293966-5c466xvz	71	4	point	point	NN
cord-293966-5c466xvz	71	5	mutations	mutation	NNS
cord-293966-5c466xvz	71	6	,	,	,
cord-293966-5c466xvz	71	7	start	start	VBP
cord-293966-5c466xvz	71	8	by	by	IN
cord-293966-5c466xvz	71	9	developing	develop	VBG
cord-293966-5c466xvz	71	10	a	a	DT
cord-293966-5c466xvz	71	11	60	60	CD
cord-293966-5c466xvz	71	12	bp	bp	NNP
cord-293966-5c466xvz	71	13	flanking	flanking	NN
cord-293966-5c466xvz	71	14	sequence	sequence	NN
cord-293966-5c466xvz	71	15	,	,	,
cord-293966-5c466xvz	71	16	calling	call	VBG
cord-293966-5c466xvz	71	17	each	each	DT
cord-293966-5c466xvz	71	18	20	20	CD
cord-293966-5c466xvz	71	19	base	base	NN
cord-293966-5c466xvz	71	20	pair	pair	NN
cord-293966-5c466xvz	71	21	section	section	NN
cord-293966-5c466xvz	71	22	as	as	IN
cord-293966-5c466xvz	71	23	A	A	NNP
cord-293966-5c466xvz	71	24	,	,	,
cord-293966-5c466xvz	71	25	B	b	NN
cord-293966-5c466xvz	71	26	,	,	,
cord-293966-5c466xvz	71	27	and	and	CC
cord-293966-5c466xvz	71	28	C.	C.	NNP
cord-293966-5c466xvz	72	1	Then	then	RB
cord-293966-5c466xvz	72	2	incorporate	incorporate	VB
cord-293966-5c466xvz	72	3	the	the	DT
cord-293966-5c466xvz	72	4	desired	desire	VBN
cord-293966-5c466xvz	72	5	mutation	mutation	NN
cord-293966-5c466xvz	72	6	at	at	IN
cord-293966-5c466xvz	72	7	the	the	DT
cord-293966-5c466xvz	72	8	end	end	NN
cord-293966-5c466xvz	72	9	of	of	IN
cord-293966-5c466xvz	72	10	section	section	NN
cord-293966-5c466xvz	72	11	B	b	NN
cord-293966-5c466xvz	72	12	(	(	-LRB-
cord-293966-5c466xvz	72	13	40th	40th	JJ
cord-293966-5c466xvz	72	14	base	base	NN
cord-293966-5c466xvz	72	15	pair	pair	NN
cord-293966-5c466xvz	72	16	,	,	,
cord-293966-5c466xvz	72	17	or	or	CC
cord-293966-5c466xvz	72	18	the	the	DT
cord-293966-5c466xvz	72	19	39th	39th	JJ
cord-293966-5c466xvz	72	20	and	and	CC
cord-293966-5c466xvz	72	21	40th	40th	JJ
cord-293966-5c466xvz	72	22	base	base	NN
cord-293966-5c466xvz	72	23	pair	pair	NN
cord-293966-5c466xvz	72	24	if	if	IN
cord-293966-5c466xvz	72	25	two	two	CD
cord-293966-5c466xvz	72	26	changes	change	NNS
cord-293966-5c466xvz	72	27	are	be	VBP
cord-293966-5c466xvz	72	28	required	require	VBN
cord-293966-5c466xvz	72	29	)	)	-RRB-
cord-293966-5c466xvz	72	30	.	.	.
cord-293966-5c466xvz	73	1	Finally	finally	RB
cord-293966-5c466xvz	73	2	,	,	,
cord-293966-5c466xvz	73	3	attach	attach	VB
cord-293966-5c466xvz	73	4	this	this	DT
cord-293966-5c466xvz	73	5	sequence	sequence	NN
cord-293966-5c466xvz	73	6	to	to	IN
cord-293966-5c466xvz	73	7	the	the	DT
cord-293966-5c466xvz	73	8	22	22	CD
cord-293966-5c466xvz	73	9	bp	bp	NNP
cord-293966-5c466xvz	73	10	Kan	Kan	NNP
cord-293966-5c466xvz	73	11	r	r	NNP
cord-293966-5c466xvz	73	12	-I	-I	NNP
cord-293966-5c466xvz	73	13	-	-	HYPH
cord-293966-5c466xvz	73	14	SceI	SceI	NNP
cord-293966-5c466xvz	73	15	sequence	sequence	NN
cord-293966-5c466xvz	73	16	below	below	RB
cord-293966-5c466xvz	73	17	to	to	TO
cord-293966-5c466xvz	73	18	create	create	VB
cord-293966-5c466xvz	73	19	your	-PRON-	PRP$
cord-293966-5c466xvz	73	20	forward	forward	JJ
cord-293966-5c466xvz	73	21	recombination	recombination	NN
cord-293966-5c466xvz	73	22	primer	primer	NN
cord-293966-5c466xvz	73	23	.	.	.
cord-293966-5c466xvz	74	1	To	to	TO
cord-293966-5c466xvz	74	2	create	create	VB
cord-293966-5c466xvz	74	3	the	the	DT
cord-293966-5c466xvz	74	4	reverse	reverse	JJ
cord-293966-5c466xvz	74	5	recombination	recombination	NN
cord-293966-5c466xvz	74	6	primer	primer	NN
cord-293966-5c466xvz	74	7	,	,	,
cord-293966-5c466xvz	74	8	create	create	VB
cord-293966-5c466xvz	74	9	a	a	DT
cord-293966-5c466xvz	74	10	new	new	JJ
cord-293966-5c466xvz	74	11	block	block	NN
cord-293966-5c466xvz	74	12	of	of	IN
cord-293966-5c466xvz	74	13	20	20	CD
cord-293966-5c466xvz	74	14	bp	bp	NN
cord-293966-5c466xvz	74	15	we	-PRON-	PRP
cord-293966-5c466xvz	74	16	will	will	MD
cord-293966-5c466xvz	74	17	call	call	VB
cord-293966-5c466xvz	74	18	section	section	NN
cord-293966-5c466xvz	74	19	D	d	NN
cord-293966-5c466xvz	74	20	0	0	NFP
cord-293966-5c466xvz	74	21	that	that	WDT
cord-293966-5c466xvz	74	22	is	be	VBZ
cord-293966-5c466xvz	74	23	the	the	DT
cord-293966-5c466xvz	74	24	reverse	reverse	JJ
cord-293966-5c466xvz	74	25	complement	complement	NN
cord-293966-5c466xvz	74	26	of	of	IN
cord-293966-5c466xvz	74	27	the	the	DT
cord-293966-5c466xvz	74	28	sequence	sequence	NN
cord-293966-5c466xvz	74	29	immediately	immediately	RB
cord-293966-5c466xvz	74	30	downstream	downstream	RB
cord-293966-5c466xvz	74	31	from	from	IN
cord-293966-5c466xvz	74	32	section	section	NN
cord-293966-5c466xvz	74	33	C.	c.	NN
cord-293966-5c466xvz	75	1	These	these	DT
cord-293966-5c466xvz	75	2	20	20	CD
cord-293966-5c466xvz	75	3	bp	bp	NNP
cord-293966-5c466xvz	75	4	will	will	MD
cord-293966-5c466xvz	75	5	be	be	VB
cord-293966-5c466xvz	75	6	followed	follow	VBN
cord-293966-5c466xvz	75	7	on	on	IN
cord-293966-5c466xvz	75	8	your	-PRON-	PRP$
cord-293966-5c466xvz	75	9	primer	primer	NN
cord-293966-5c466xvz	75	10	by	by	IN
cord-293966-5c466xvz	75	11	sections	section	NNS
cord-293966-5c466xvz	75	12	C	C	NNP
cord-293966-5c466xvz	75	13	0	0	CD
cord-293966-5c466xvz	75	14	and	and	CC
cord-293966-5c466xvz	75	15	B	B	NNP
cord-293966-5c466xvz	75	16	0	0	CD
cord-293966-5c466xvz	75	17	,	,	,
cord-293966-5c466xvz	75	18	the	the	DT
cord-293966-5c466xvz	75	19	reverse	reverse	NN
cord-293966-5c466xvz	75	20	complements	complement	VBZ
cord-293966-5c466xvz	75	21	to	to	IN
cord-293966-5c466xvz	75	22	sections	section	NNS
cord-293966-5c466xvz	75	23	B	b	NN
cord-293966-5c466xvz	75	24	and	and	CC
cord-293966-5c466xvz	75	25	C	C	NNP
cord-293966-5c466xvz	75	26	,	,	,
cord-293966-5c466xvz	75	27	with	with	IN
cord-293966-5c466xvz	75	28	B	b	NN
cord-293966-5c466xvz	75	29	0	0	CD
cord-293966-5c466xvz	75	30	containing	contain	VBG
cord-293966-5c466xvz	75	31	the	the	DT
cord-293966-5c466xvz	75	32	desired	desire	VBN
cord-293966-5c466xvz	75	33	mutations	mutation	NNS
cord-293966-5c466xvz	75	34	.	.	.
cord-293966-5c466xvz	76	1	Finally	finally	RB
cord-293966-5c466xvz	76	2	,	,	,
cord-293966-5c466xvz	76	3	add	add	VB
cord-293966-5c466xvz	76	4	the	the	DT
cord-293966-5c466xvz	76	5	23	23	CD
cord-293966-5c466xvz	76	6	bp	bp	NNP
cord-293966-5c466xvz	76	7	Kan	Kan	NNP
cord-293966-5c466xvz	76	8	r	r	NNP
cord-293966-5c466xvz	76	9	-I	-I	NNP
cord-293966-5c466xvz	76	10	-	-	HYPH
cord-293966-5c466xvz	76	11	SceI	SceI	NNP
cord-293966-5c466xvz	76	12	sequence	sequence	NN
cord-293966-5c466xvz	76	13	to	to	TO
cord-293966-5c466xvz	76	14	finish	finish	VB
cord-293966-5c466xvz	76	15	the	the	DT
cord-293966-5c466xvz	76	16	reverse	reverse	JJ
cord-293966-5c466xvz	76	17	primer	primer	NN
cord-293966-5c466xvz	76	18	.	.	.
cord-293966-5c466xvz	77	1	During	during	IN
cord-293966-5c466xvz	77	2	negative	negative	JJ
cord-293966-5c466xvz	77	3	selection	selection	NN
cord-293966-5c466xvz	77	4	,	,	,
cord-293966-5c466xvz	77	5	sections	section	NNS
cord-293966-5c466xvz	77	6	B	B	NNP
cord-293966-5c466xvz	77	7	/	/	SYM
cord-293966-5c466xvz	77	8	C	C	NNP
cord-293966-5c466xvz	77	9	will	will	MD
cord-293966-5c466xvz	77	10	recombine	recombine	VB
cord-293966-5c466xvz	77	11	with	with	IN
cord-293966-5c466xvz	77	12	B	b	NN
cord-293966-5c466xvz	77	13	0	0	CD
cord-293966-5c466xvz	77	14	/C	/c	SYM
cord-293966-5c466xvz	77	15	0	0	CD
cord-293966-5c466xvz	77	16	leading	lead	VBG
cord-293966-5c466xvz	77	17	to	to	IN
cord-293966-5c466xvz	77	18	the	the	DT
cord-293966-5c466xvz	77	19	loss	loss	NN
cord-293966-5c466xvz	77	20	of	of	IN
cord-293966-5c466xvz	77	21	the	the	DT
cord-293966-5c466xvz	77	22	Kan	Kan	NNP
cord-293966-5c466xvz	77	23	R	R	NNP
cord-293966-5c466xvz	77	24	-I	-I	NNP
cord-293966-5c466xvz	77	25	-	-	HYPH
cord-293966-5c466xvz	77	26	SceI	SceI	NNP
cord-293966-5c466xvz	77	27	cassette	cassette	NN
cord-293966-5c466xvz	77	28	(	(	-LRB-
cord-293966-5c466xvz	77	29	Fig	Fig	NNP
cord-293966-5c466xvz	77	30	.	.	.
cord-293966-5c466xvz	77	31	1	1	CD
cord-293966-5c466xvz	77	32	g	g	NN
cord-293966-5c466xvz	77	33	)	)	-RRB-
cord-293966-5c466xvz	77	34	.	.	.
cord-293966-5c466xvz	78	1	For	for	IN
cord-293966-5c466xvz	78	2	deletion	deletion	NN
cord-293966-5c466xvz	78	3	mutants	mutant	NNS
cord-293966-5c466xvz	78	4	,	,	,
cord-293966-5c466xvz	78	5	leave	leave	VB
cord-293966-5c466xvz	78	6	out	out	RP
cord-293966-5c466xvz	78	7	the	the	DT
cord-293966-5c466xvz	78	8	desired	desire	VBN
cord-293966-5c466xvz	78	9	sequence	sequence	NN
cord-293966-5c466xvz	78	10	from	from	IN
cord-293966-5c466xvz	78	11	your	-PRON-	PRP$
cord-293966-5c466xvz	78	12	primers	primer	NNS
cord-293966-5c466xvz	78	13	.	.	.
cord-293966-5c466xvz	79	1	For	for	IN
cord-293966-5c466xvz	79	2	instance	instance	NN
cord-293966-5c466xvz	79	3	,	,	,
cord-293966-5c466xvz	79	4	to	to	TO
cord-293966-5c466xvz	79	5	delete	delete	VB
cord-293966-5c466xvz	79	6	sections	section	NNS
cord-293966-5c466xvz	79	7	D	d	NN
cord-293966-5c466xvz	79	8	/	/	SYM
cord-293966-5c466xvz	79	9	E	e	NN
cord-293966-5c466xvz	79	10	/	/	SYM
cord-293966-5c466xvz	79	11	F	F	NNP
cord-293966-5c466xvz	79	12	,	,	,
cord-293966-5c466xvz	79	13	simply	simply	RB
cord-293966-5c466xvz	79	14	create	create	VB
cord-293966-5c466xvz	79	15	the	the	DT
cord-293966-5c466xvz	79	16	forward	forward	JJ
cord-293966-5c466xvz	79	17	primer	primer	NN
cord-293966-5c466xvz	79	18	with	with	IN
cord-293966-5c466xvz	79	19	sections	section	NNS
cord-293966-5c466xvz	79	20	A	a	DT
cord-293966-5c466xvz	79	21	/	/	SYM
cord-293966-5c466xvz	79	22	B/	B/	NNP
cord-293966-5c466xvz	79	23	C	C	NNP
cord-293966-5c466xvz	79	24	,	,	,
cord-293966-5c466xvz	79	25	and	and	CC
cord-293966-5c466xvz	79	26	the	the	DT
cord-293966-5c466xvz	79	27	reverse	reverse	JJ
cord-293966-5c466xvz	79	28	primer	primer	NN
cord-293966-5c466xvz	79	29	with	with	IN
cord-293966-5c466xvz	79	30	sections	section	NNS
cord-293966-5c466xvz	79	31	G	g	NN
cord-293966-5c466xvz	79	32	0	0	CD
cord-293966-5c466xvz	79	33	/C	/c	NN
cord-293966-5c466xvz	79	34	0	0	CD
cord-293966-5c466xvz	79	35	/B	/b	NN
cord-293966-5c466xvz	79	36	0	0	NFP
cord-293966-5c466xvz	79	37	.	.	.
cord-293966-5c466xvz	80	1	Insertions	insertion	NNS
cord-293966-5c466xvz	80	2	of	of	IN
cord-293966-5c466xvz	80	3	small	small	JJ
cord-293966-5c466xvz	80	4	sequences	sequence	NNS
cord-293966-5c466xvz	80	5	can	can	MD
cord-293966-5c466xvz	80	6	be	be	VB
cord-293966-5c466xvz	80	7	achieved	achieve	VBN
cord-293966-5c466xvz	80	8	by	by	IN
cord-293966-5c466xvz	80	9	adding	add	VBG
cord-293966-5c466xvz	80	10	the	the	DT
cord-293966-5c466xvz	80	11	entire	entire	JJ
cord-293966-5c466xvz	80	12	insertion	insertion	NN
cord-293966-5c466xvz	80	13	sequence	sequence	NN
cord-293966-5c466xvz	80	14	at	at	IN
cord-293966-5c466xvz	80	15	the	the	DT
cord-293966-5c466xvz	80	16	3	3	CD
cord-293966-5c466xvz	80	17	0	0	CD
cord-293966-5c466xvz	80	18	end	end	NN
cord-293966-5c466xvz	80	19	of	of	IN
cord-293966-5c466xvz	80	20	the	the	DT
cord-293966-5c466xvz	80	21	forward	forward	JJ
cord-293966-5c466xvz	80	22	primer	primer	NN
cord-293966-5c466xvz	80	23	,	,	,
cord-293966-5c466xvz	80	24	and	and	CC
cord-293966-5c466xvz	80	25	at	at	RB
cord-293966-5c466xvz	80	26	least	least	JJS
cord-293966-5c466xvz	80	27	50	50	CD
cord-293966-5c466xvz	80	28	bp	bp	NNS
cord-293966-5c466xvz	80	29	of	of	IN
cord-293966-5c466xvz	80	30	reverse	reverse	JJ
cord-293966-5c466xvz	80	31	complement	complement	NN
cord-293966-5c466xvz	80	32	sequence	sequence	NN
cord-293966-5c466xvz	80	33	at	at	IN
cord-293966-5c466xvz	80	34	the	the	DT
cord-293966-5c466xvz	80	35	3	3	CD
cord-293966-5c466xvz	80	36	0	0	CD
cord-293966-5c466xvz	80	37	end	end	NN
cord-293966-5c466xvz	80	38	of	of	IN
cord-293966-5c466xvz	80	39	the	the	DT
cord-293966-5c466xvz	80	40	reverse	reverse	JJ
cord-293966-5c466xvz	80	41	primer	primer	NN
cord-293966-5c466xvz	80	42	(	(	-LRB-
cord-293966-5c466xvz	80	43	Fig	fig	NN
cord-293966-5c466xvz	80	44	.	.	NNP
cord-293966-5c466xvz	80	45	2	2	CD
cord-293966-5c466xvz	80	46	)	)	-RRB-
cord-293966-5c466xvz	80	47	.	.	.
cord-293966-5c466xvz	81	1	Larger	large	JJR
cord-293966-5c466xvz	81	2	insertions	insertion	NNS
cord-293966-5c466xvz	81	3	may	may	MD
cord-293966-5c466xvz	81	4	require	require	VB
cord-293966-5c466xvz	81	5	the	the	DT
cord-293966-5c466xvz	81	6	development	development	NN
cord-293966-5c466xvz	81	7	of	of	IN
cord-293966-5c466xvz	81	8	a	a	DT
cord-293966-5c466xvz	81	9	full	full	JJ
cord-293966-5c466xvz	81	10	plasmid	plasmid	NN
cord-293966-5c466xvz	81	11	,	,	,
cord-293966-5c466xvz	81	12	or	or	CC
cord-293966-5c466xvz	81	13	potentially	potentially	RB
cord-293966-5c466xvz	81	14	the	the	DT
cord-293966-5c466xvz	81	15	use	use	NN
cord-293966-5c466xvz	81	16	of	of	IN
cord-293966-5c466xvz	81	17	nested	nested	JJ
cord-293966-5c466xvz	81	18	PCRs	pcr	NNS
cord-293966-5c466xvz	81	19	.	.	.
cord-293966-5c466xvz	82	1	For	for	IN
cord-293966-5c466xvz	82	2	additional	additional	JJ
cord-293966-5c466xvz	82	3	details	detail	NNS
cord-293966-5c466xvz	82	4	,	,	,
cord-293966-5c466xvz	82	5	see	see	VB
cord-293966-5c466xvz	82	6	ref	ref	NN
cord-293966-5c466xvz	82	7	.	.	.
cord-293966-5c466xvz	83	1	[	[	-LRB-
cord-293966-5c466xvz	83	2	18	18	CD
cord-293966-5c466xvz	83	3	]	]	-RRB-
cord-293966-5c466xvz	83	4	.	.	.
cord-293966-5c466xvz	84	1	While	while	IN
cord-293966-5c466xvz	84	2	designing	design	VBG
cord-293966-5c466xvz	84	3	recombination	recombination	NN
cord-293966-5c466xvz	84	4	primers	primer	NNS
cord-293966-5c466xvz	84	5	(	(	-LRB-
cord-293966-5c466xvz	84	6	indicated	indicate	VBN
cord-293966-5c466xvz	84	7	below	below	IN
cord-293966-5c466xvz	84	8	)	)	-RRB-
cord-293966-5c466xvz	84	9	,	,	,
cord-293966-5c466xvz	84	10	remember	remember	VB
cord-293966-5c466xvz	84	11	to	to	TO
cord-293966-5c466xvz	84	12	also	also	RB
cord-293966-5c466xvz	84	13	order	order	VB
cord-293966-5c466xvz	84	14	short	short	JJ
cord-293966-5c466xvz	84	15	primers	primer	NNS
cord-293966-5c466xvz	84	16	about	about	RB
cord-293966-5c466xvz	84	17	100	100	CD
cord-293966-5c466xvz	84	18	-	-	SYM
cord-293966-5c466xvz	84	19	200	200	CD
cord-293966-5c466xvz	84	20	bp	bp	NNP
cord-293966-5c466xvz	84	21	outside	outside	IN
cord-293966-5c466xvz	84	22	of	of	IN
cord-293966-5c466xvz	84	23	the	the	DT
cord-293966-5c466xvz	84	24	insertion	insertion	NN
cord-293966-5c466xvz	84	25	site	site	NN
cord-293966-5c466xvz	84	26	to	to	TO
cord-293966-5c466xvz	84	27	check	check	VB
cord-293966-5c466xvz	84	28	for	for	IN
cord-293966-5c466xvz	84	29	the	the	DT
cord-293966-5c466xvz	84	30	proper	proper	JJ
cord-293966-5c466xvz	84	31	insertion	insertion	NN
cord-293966-5c466xvz	84	32	of	of	IN
cord-293966-5c466xvz	84	33	the	the	DT
cord-293966-5c466xvz	84	34	gene	gene	NN
cord-293966-5c466xvz	84	35	cassette	cassette	NN
cord-293966-5c466xvz	84	36	by	by	IN
cord-293966-5c466xvz	84	37	PCR	PCR	NNP
cord-293966-5c466xvz	84	38	.	.	.
cord-293966-5c466xvz	85	1	2	2	LS
cord-293966-5c466xvz	85	2	.	.	.
cord-293966-5c466xvz	86	1	Set	Set	VBN
cord-293966-5c466xvz	86	2	up	up	RP
cord-293966-5c466xvz	86	3	PCR	pcr	NN
cord-293966-5c466xvz	86	4	reaction	reaction	NN
cord-293966-5c466xvz	86	5	and	and	CC
cord-293966-5c466xvz	86	6	perform	perform	VB
cord-293966-5c466xvz	86	7	reaction	reaction	NN
cord-293966-5c466xvz	86	8	according	accord	VBG
cord-293966-5c466xvz	86	9	to	to	IN
cord-293966-5c466xvz	86	10	manufacturer	manufacturer	NN
cord-293966-5c466xvz	86	11	's	's	POS
cord-293966-5c466xvz	86	12	protocol	protocol	NN
cord-293966-5c466xvz	86	13	with	with	IN
cord-293966-5c466xvz	86	14	following	follow	VBG
cord-293966-5c466xvz	86	15	modifications	modification	NNS
cord-293966-5c466xvz	86	16	(	(	-LRB-
cord-293966-5c466xvz	86	17	Fig	fig	NN
cord-293966-5c466xvz	86	18	.	.	.
cord-293966-5c466xvz	86	19	1b	1b	CD
cord-293966-5c466xvz	86	20	)	)	-RRB-
cord-293966-5c466xvz	86	21	.	.	.
cord-293966-5c466xvz	87	1	Fig	Fig	NNP
cord-293966-5c466xvz	87	2	.	.	NNP
cord-293966-5c466xvz	87	3	2	2	CD
cord-293966-5c466xvz	87	4	Model	model	NN
cord-293966-5c466xvz	87	5	of	of	IN
cord-293966-5c466xvz	87	6	the	the	DT
cord-293966-5c466xvz	87	7	PCR	PCR	NNP
cord-293966-5c466xvz	87	8	product	product	NN
cord-293966-5c466xvz	87	9	used	use	VBN
cord-293966-5c466xvz	87	10	for	for	IN
cord-293966-5c466xvz	87	11	inserting	insert	VBG
cord-293966-5c466xvz	87	12	specific	specific	JJ
cord-293966-5c466xvz	87	13	sequences	sequence	NNS
cord-293966-5c466xvz	87	14	into	into	IN
cord-293966-5c466xvz	87	15	BACs	bac	NNS
cord-293966-5c466xvz	87	16	using	use	VBG
cord-293966-5c466xvz	87	17	lambda	lambda	NN
cord-293966-5c466xvz	87	18	red	red	JJ
cord-293966-5c466xvz	87	19	recombination	recombination	NN
cord-293966-5c466xvz	87	20	.	.	.
cord-293966-5c466xvz	88	1	The	the	DT
cord-293966-5c466xvz	88	2	full	full	JJ
cord-293966-5c466xvz	88	3	sequence	sequence	NN
cord-293966-5c466xvz	88	4	for	for	IN
cord-293966-5c466xvz	88	5	insertion	insertion	NN
cord-293966-5c466xvz	88	6	is	be	VBZ
cord-293966-5c466xvz	88	7	incorporated	incorporate	VBN
cord-293966-5c466xvz	88	8	at	at	IN
cord-293966-5c466xvz	88	9	the	the	DT
cord-293966-5c466xvz	88	10	5	5	CD
cord-293966-5c466xvz	88	11	0	0	CD
cord-293966-5c466xvz	88	12	end	end	NN
cord-293966-5c466xvz	88	13	of	of	IN
cord-293966-5c466xvz	88	14	the	the	DT
cord-293966-5c466xvz	88	15	Kan	Kan	NNP
cord-293966-5c466xvz	88	16	R	R	NNP
cord-293966-5c466xvz	88	17	-I	-I	NNP
cord-293966-5c466xvz	88	18	-	-	HYPH
cord-293966-5c466xvz	88	19	SceI	SceI	NNP
cord-293966-5c466xvz	88	20	cassette	cassette	NN
cord-293966-5c466xvz	88	21	while	while	IN
cord-293966-5c466xvz	88	22	at	at	RB
cord-293966-5c466xvz	88	23	least	least	JJS
cord-293966-5c466xvz	88	24	50	50	CD
cord-293966-5c466xvz	88	25	nt	nt	RB
cord-293966-5c466xvz	88	26	of	of	IN
cord-293966-5c466xvz	88	27	sequence	sequence	NN
cord-293966-5c466xvz	88	28	homologous	homologous	JJ
cord-293966-5c466xvz	88	29	to	to	IN
cord-293966-5c466xvz	88	30	the	the	DT
cord-293966-5c466xvz	88	31	3	3	CD
cord-293966-5c466xvz	88	32	0	0	CD
cord-293966-5c466xvz	88	33	end	end	NN
cord-293966-5c466xvz	88	34	of	of	IN
cord-293966-5c466xvz	88	35	the	the	DT
cord-293966-5c466xvz	88	36	insertion	insertion	NN
cord-293966-5c466xvz	88	37	sequence	sequence	NN
cord-293966-5c466xvz	88	38	is	be	VBZ
cord-293966-5c466xvz	88	39	incorporated	incorporate	VBN
cord-293966-5c466xvz	88	40	at	at	IN
cord-293966-5c466xvz	88	41	the	the	DT
cord-293966-5c466xvz	88	42	3	3	CD
cord-293966-5c466xvz	88	43	0	0	CD
cord-293966-5c466xvz	88	44	end	end	NN
cord-293966-5c466xvz	88	45	of	of	IN
cord-293966-5c466xvz	88	46	this	this	DT
cord-293966-5c466xvz	88	47	cassette	cassette	NN
cord-293966-5c466xvz	88	48	.	.	.
cord-293966-5c466xvz	89	1	Surrounding	surround	VBG
cord-293966-5c466xvz	89	2	these	these	DT
cord-293966-5c466xvz	89	3	sequences	sequence	NNS
cord-293966-5c466xvz	89	4	are	be	VBP
cord-293966-5c466xvz	89	5	50	50	CD
cord-293966-5c466xvz	89	6	nt	nt	RB
cord-293966-5c466xvz	89	7	of	of	IN
cord-293966-5c466xvz	89	8	sequence	sequence	NN
cord-293966-5c466xvz	89	9	homologous	homologous	JJ
cord-293966-5c466xvz	89	10	to	to	IN
cord-293966-5c466xvz	89	11	the	the	DT
cord-293966-5c466xvz	89	12	viral	viral	JJ
cord-293966-5c466xvz	89	13	sequence	sequence	NN
cord-293966-5c466xvz	89	14	where	where	WRB
cord-293966-5c466xvz	89	15	the	the	DT
cord-293966-5c466xvz	89	16	sequence	sequence	NN
cord-293966-5c466xvz	89	17	is	be	VBZ
cord-293966-5c466xvz	89	18	to	to	TO
cord-293966-5c466xvz	89	19	be	be	VB
cord-293966-5c466xvz	89	20	inserted	insert	VBN
cord-293966-5c466xvz	89	21	3	3	CD
cord-293966-5c466xvz	89	22	.	.	.
cord-293966-5c466xvz	90	1	For	for	IN
cord-293966-5c466xvz	90	2	a	a	DT
cord-293966-5c466xvz	90	3	PCR	pcr	NN
cord-293966-5c466xvz	90	4	template	template	NN
cord-293966-5c466xvz	90	5	,	,	,
cord-293966-5c466xvz	90	6	use~50	use~50	CD
cord-293966-5c466xvz	90	7	ng	ng	NN
cord-293966-5c466xvz	90	8	of	of	IN
cord-293966-5c466xvz	90	9	the	the	DT
cord-293966-5c466xvz	90	10	pEP	pEP	NNP
cord-293966-5c466xvz	90	11	-	-	HYPH
cord-293966-5c466xvz	90	12	KanS	KanS	NNP
cord-293966-5c466xvz	90	13	plasmid	plasmid	NN
cord-293966-5c466xvz	90	14	.	.	.
cord-293966-5c466xvz	91	1	4	4	LS
cord-293966-5c466xvz	91	2	.	.	.
cord-293966-5c466xvz	92	1	Use	Use	NNP
cord-293966-5c466xvz	92	2	1	1	CD
cord-293966-5c466xvz	92	3	μL	μl	CD
cord-293966-5c466xvz	92	4	high	high	JJ
cord-293966-5c466xvz	92	5	-	-	HYPH
cord-293966-5c466xvz	92	6	fidelity	fidelity	NN
cord-293966-5c466xvz	92	7	polymerase	polymerase	NN
cord-293966-5c466xvz	92	8	(	(	-LRB-
cord-293966-5c466xvz	92	9	see	see	VB
cord-293966-5c466xvz	92	10	Note	Note	NNP
cord-293966-5c466xvz	92	11	10	10	CD
cord-293966-5c466xvz	92	12	)	)	-RRB-
cord-293966-5c466xvz	92	13	.	.	.
cord-293966-5c466xvz	93	1	procedure	procedure	NN
cord-293966-5c466xvz	93	2	lowering	lower	VBG
cord-293966-5c466xvz	93	3	the	the	DT
cord-293966-5c466xvz	93	4	annealing	anneal	VBG
cord-293966-5c466xvz	93	5	temperature	temperature	NN
cord-293966-5c466xvz	93	6	by	by	IN
cord-293966-5c466xvz	93	7	1	1	CD
cord-293966-5c466xvz	93	8	C	c	NN
cord-293966-5c466xvz	93	9	starting	start	VBG
cord-293966-5c466xvz	93	10	at	at	IN
cord-293966-5c466xvz	93	11	68	68	CD
cord-293966-5c466xvz	93	12	C	c	NN
cord-293966-5c466xvz	93	13	and	and	CC
cord-293966-5c466xvz	93	14	continuing	continue	VBG
cord-293966-5c466xvz	93	15	the	the	DT
cord-293966-5c466xvz	93	16	PCR	PCR	NNP
cord-293966-5c466xvz	93	17	reaction	reaction	NN
cord-293966-5c466xvz	93	18	for	for	IN
cord-293966-5c466xvz	93	19	25	25	CD
cord-293966-5c466xvz	93	20	cycles	cycle	NNS
cord-293966-5c466xvz	93	21	.	.	.
cord-293966-5c466xvz	94	1	Using	use	VBG
cord-293966-5c466xvz	94	2	this	this	DT
cord-293966-5c466xvz	94	3	method	method	NN
cord-293966-5c466xvz	94	4	we	-PRON-	PRP
cord-293966-5c466xvz	94	5	rarely	rarely	RB
cord-293966-5c466xvz	94	6	see	see	VBP
cord-293966-5c466xvz	94	7	spurious	spurious	JJ
cord-293966-5c466xvz	94	8	PCR	PCR	NNP
cord-293966-5c466xvz	94	9	products	product	NNS
cord-293966-5c466xvz	94	10	.	.	.
cord-293966-5c466xvz	95	1	6	6	LS
cord-293966-5c466xvz	95	2	.	.	.
cord-293966-5c466xvz	96	1	Analyze	analyze	VB
cord-293966-5c466xvz	96	2	the	the	DT
cord-293966-5c466xvz	96	3	PCR	PCR	NNP
cord-293966-5c466xvz	96	4	product	product	NN
cord-293966-5c466xvz	96	5	on	on	IN
cord-293966-5c466xvz	96	6	an	an	DT
cord-293966-5c466xvz	96	7	agarose	agarose	JJ
cord-293966-5c466xvz	96	8	gel	gel	NN
cord-293966-5c466xvz	96	9	with	with	IN
cord-293966-5c466xvz	96	10	ethidium	ethidium	NN
cord-293966-5c466xvz	96	11	bromide	bromide	NN
cord-293966-5c466xvz	96	12	and	and	CC
cord-293966-5c466xvz	96	13	image	image	VB
cord-293966-5c466xvz	96	14	it	-PRON-	PRP
cord-293966-5c466xvz	96	15	using	use	VBG
cord-293966-5c466xvz	96	16	a	a	DT
cord-293966-5c466xvz	96	17	UV	uv	NN
cord-293966-5c466xvz	96	18	-	-	HYPH
cord-293966-5c466xvz	96	19	gel	gel	NN
cord-293966-5c466xvz	96	20	box	box	NN
cord-293966-5c466xvz	96	21	and	and	CC
cord-293966-5c466xvz	96	22	gel	gel	NN
cord-293966-5c466xvz	96	23	-	-	HYPH
cord-293966-5c466xvz	96	24	imaging	image	VBG
cord-293966-5c466xvz	96	25	software	software	NN
cord-293966-5c466xvz	96	26	.	.	.
cord-293966-5c466xvz	97	1	PCR	PCR	NNP
cord-293966-5c466xvz	97	2	product	product	NN
cord-293966-5c466xvz	97	3	should	should	MD
cord-293966-5c466xvz	97	4	be~1.2	be~1.2	VB
cord-293966-5c466xvz	97	5	kb	kb	NNP
cord-293966-5c466xvz	97	6	.	.	NNP
cord-293966-5c466xvz	97	7	7	7	CD
cord-293966-5c466xvz	97	8	.	.	.
cord-293966-5c466xvz	98	1	Purify	purify	VB
cord-293966-5c466xvz	98	2	the	the	DT
cord-293966-5c466xvz	98	3	PCR	PCR	NNP
cord-293966-5c466xvz	98	4	product	product	NN
cord-293966-5c466xvz	98	5	using	use	VBG
cord-293966-5c466xvz	98	6	the	the	DT
cord-293966-5c466xvz	98	7	PureLink	PureLink	NNP
cord-293966-5c466xvz	98	8	PCR	PCR	NNP
cord-293966-5c466xvz	98	9	purification	purification	NN
cord-293966-5c466xvz	98	10	kit	kit	NN
cord-293966-5c466xvz	98	11	(	(	-LRB-
cord-293966-5c466xvz	98	12	see	see	VB
cord-293966-5c466xvz	98	13	Note	Note	NNP
cord-293966-5c466xvz	98	14	5	5	CD
cord-293966-5c466xvz	98	15	)	)	-RRB-
cord-293966-5c466xvz	98	16	.	.	.
cord-293966-5c466xvz	99	1	Use	use	VB
cord-293966-5c466xvz	99	2	binding	bind	VBG
cord-293966-5c466xvz	99	3	buffer	buffer	NN
cord-293966-5c466xvz	99	4	B3	b3	NN
cord-293966-5c466xvz	99	5	according	accord	VBG
cord-293966-5c466xvz	99	6	to	to	IN
cord-293966-5c466xvz	99	7	manufacturer	manufacturer	NN
cord-293966-5c466xvz	99	8	's	's	POS
cord-293966-5c466xvz	99	9	protocol	protocol	NN
cord-293966-5c466xvz	99	10	to	to	TO
cord-293966-5c466xvz	99	11	remove	remove	VB
cord-293966-5c466xvz	99	12	primer	primer	NN
cord-293966-5c466xvz	99	13	dimers	dimer	NNS
cord-293966-5c466xvz	99	14	from	from	IN
cord-293966-5c466xvz	99	15	the	the	DT
cord-293966-5c466xvz	99	16	mixture	mixture	NN
cord-293966-5c466xvz	99	17	.	.	.
cord-293966-5c466xvz	100	1	Elute	Elute	NNP
cord-293966-5c466xvz	100	2	DNA	DNA	NNP
cord-293966-5c466xvz	100	3	into	into	IN
cord-293966-5c466xvz	100	4	44	44	CD
cord-293966-5c466xvz	100	5	μL	μL	NNS
cord-293966-5c466xvz	100	6	of	of	IN
cord-293966-5c466xvz	100	7	water	water	NN
cord-293966-5c466xvz	100	8	.	.	.
cord-293966-5c466xvz	101	1	8	8	CD
cord-293966-5c466xvz	101	2	.	.	.
cord-293966-5c466xvz	102	1	DpnI	DpnI	NNP
cord-293966-5c466xvz	102	2	digest	digest	VBP
cord-293966-5c466xvz	102	3	the	the	DT
cord-293966-5c466xvz	102	4	pEP	pEP	NNP
cord-293966-5c466xvz	102	5	-	-	HYPH
cord-293966-5c466xvz	102	6	KanS	KanS	NNP
cord-293966-5c466xvz	102	7	plasmid	plasmid	NN
cord-293966-5c466xvz	102	8	in	in	IN
cord-293966-5c466xvz	102	9	the	the	DT
cord-293966-5c466xvz	102	10	purified	purify	VBN
cord-293966-5c466xvz	102	11	PCR	PCR	NNP
cord-293966-5c466xvz	102	12	product	product	NN
cord-293966-5c466xvz	102	13	(	(	-LRB-
cord-293966-5c466xvz	102	14	Fig	Fig	NNP
cord-293966-5c466xvz	102	15	.	.	.
cord-293966-5c466xvz	102	16	1b	1b	CD
cord-293966-5c466xvz	102	17	)	)	-RRB-
cord-293966-5c466xvz	102	18	.	.	.
cord-293966-5c466xvz	103	1	DpnI	DpnI	NNP
cord-293966-5c466xvz	103	2	specifically	specifically	RB
cord-293966-5c466xvz	103	3	cleaves	cleave	VBZ
cord-293966-5c466xvz	103	4	methylated	methylate	VBN
cord-293966-5c466xvz	103	5	DNA	dna	NN
cord-293966-5c466xvz	103	6	and	and	CC
cord-293966-5c466xvz	103	7	is	be	VBZ
cord-293966-5c466xvz	103	8	needed	need	VBN
cord-293966-5c466xvz	103	9	to	to	TO
cord-293966-5c466xvz	103	10	digest	digest	VB
cord-293966-5c466xvz	103	11	the	the	DT
cord-293966-5c466xvz	103	12	pEP	pEP	NNP
cord-293966-5c466xvz	103	13	-	-	HYPH
cord-293966-5c466xvz	103	14	KanS	KanS	NNP
cord-293966-5c466xvz	103	15	plasmid	plasmid	NN
cord-293966-5c466xvz	103	16	used	use	VBN
cord-293966-5c466xvz	103	17	in	in	IN
cord-293966-5c466xvz	103	18	the	the	DT
cord-293966-5c466xvz	103	19	PCR	PCR	NNP
cord-293966-5c466xvz	103	20	reaction	reaction	NN
cord-293966-5c466xvz	103	21	.	.	.
cord-293966-5c466xvz	104	1	It	-PRON-	PRP
cord-293966-5c466xvz	104	2	has	have	VBZ
cord-293966-5c466xvz	104	3	a	a	DT
cord-293966-5c466xvz	104	4	4	4	CD
cord-293966-5c466xvz	104	5	bp	bp	NNP
cord-293966-5c466xvz	104	6	recognition	recognition	NN
cord-293966-5c466xvz	104	7	site	site	NN
cord-293966-5c466xvz	104	8	so	so	IN
cord-293966-5c466xvz	104	9	it	-PRON-	PRP
cord-293966-5c466xvz	104	10	should	should	MD
cord-293966-5c466xvz	104	11	cleave	cleave	VB
cord-293966-5c466xvz	104	12	DNA	DNA	NNP
cord-293966-5c466xvz	104	13	approximately	approximately	RB
cord-293966-5c466xvz	104	14	every	every	DT
cord-293966-5c466xvz	104	15	250	250	CD
cord-293966-5c466xvz	104	16	bp	bp	NNP
cord-293966-5c466xvz	104	17	.	.	.
cord-293966-5c466xvz	105	1	Without	without	IN
cord-293966-5c466xvz	105	2	this	this	DT
cord-293966-5c466xvz	105	3	digestion	digestion	NN
cord-293966-5c466xvz	105	4	all	all	DT
cord-293966-5c466xvz	105	5	of	of	IN
cord-293966-5c466xvz	105	6	your	-PRON-	PRP$
cord-293966-5c466xvz	105	7	transformants	transformant	NNS
cord-293966-5c466xvz	105	8	will	will	MD
cord-293966-5c466xvz	105	9	maintain	maintain	VB
cord-293966-5c466xvz	105	10	the	the	DT
cord-293966-5c466xvz	105	11	pEP	pEP	NNP
cord-293966-5c466xvz	105	12	-	-	HYPH
cord-293966-5c466xvz	105	13	KanS	KanS	NNP
cord-293966-5c466xvz	105	14	plasmid	plasmid	NN
cord-293966-5c466xvz	105	15	as	as	IN
cord-293966-5c466xvz	105	16	its	-PRON-	PRP$
cord-293966-5c466xvz	105	17	transformation	transformation	NN
cord-293966-5c466xvz	105	18	is	be	VBZ
cord-293966-5c466xvz	105	19	much	much	RB
cord-293966-5c466xvz	105	20	more	more	RBR
cord-293966-5c466xvz	105	21	efficient	efficient	JJ
cord-293966-5c466xvz	105	22	than	than	IN
cord-293966-5c466xvz	105	23	the	the	DT
cord-293966-5c466xvz	105	24	recombination	recombination	NN
cord-293966-5c466xvz	105	25	of	of	IN
cord-293966-5c466xvz	105	26	the	the	DT
cord-293966-5c466xvz	105	27	PCR	PCR	NNP
cord-293966-5c466xvz	105	28	product	product	NN
cord-293966-5c466xvz	105	29	.	.	.
cord-293966-5c466xvz	106	1	Incubate	incubate	VB
cord-293966-5c466xvz	106	2	for	for	IN
cord-293966-5c466xvz	106	3	1	1	CD
cord-293966-5c466xvz	106	4	-	-	SYM
cord-293966-5c466xvz	106	5	3	3	CD
cord-293966-5c466xvz	106	6	h	h	NN
cord-293966-5c466xvz	106	7	at	at	IN
cord-293966-5c466xvz	106	8	37	37	CD
cord-293966-5c466xvz	106	9	C.	C.	NNP
cord-293966-5c466xvz	106	10	4	4	CD
cord-293966-5c466xvz	106	11	.	.	.
cord-293966-5c466xvz	107	1	Carefully	carefully	RB
cord-293966-5c466xvz	107	2	transfer	transfer	VB
cord-293966-5c466xvz	107	3	the	the	DT
cord-293966-5c466xvz	107	4	mixture	mixture	NN
cord-293966-5c466xvz	107	5	into	into	IN
cord-293966-5c466xvz	107	6	the	the	DT
cord-293966-5c466xvz	107	7	groove	groove	NN
cord-293966-5c466xvz	107	8	of	of	IN
cord-293966-5c466xvz	107	9	the	the	DT
cord-293966-5c466xvz	107	10	electroporation	electroporation	NN
cord-293966-5c466xvz	107	11	cuvette	cuvette	NN
cord-293966-5c466xvz	107	12	on	on	IN
cord-293966-5c466xvz	107	13	ice	ice	NN
cord-293966-5c466xvz	107	14	.	.	.
cord-293966-5c466xvz	108	1	5	5	LS
cord-293966-5c466xvz	108	2	.	.	.
cord-293966-5c466xvz	109	1	Wipe	wipe	VB
cord-293966-5c466xvz	109	2	any	any	DT
cord-293966-5c466xvz	109	3	ice	ice	NN
cord-293966-5c466xvz	109	4	water	water	NN
cord-293966-5c466xvz	109	5	from	from	IN
cord-293966-5c466xvz	109	6	outside	outside	RB
cord-293966-5c466xvz	109	7	of	of	IN
cord-293966-5c466xvz	109	8	cuvette	cuvette	NN
cord-293966-5c466xvz	109	9	and	and	CC
cord-293966-5c466xvz	109	10	pulse	pulse	NN
cord-293966-5c466xvz	109	11	at	at	IN
cord-293966-5c466xvz	109	12	25	25	CD
cord-293966-5c466xvz	109	13	μF	μf	CD
cord-293966-5c466xvz	109	14	,	,	,
cord-293966-5c466xvz	109	15	6	6	CD
cord-293966-5c466xvz	109	16	.	.	.
cord-293966-5c466xvz	110	1	Recover	recover	VB
cord-293966-5c466xvz	110	2	by	by	IN
cord-293966-5c466xvz	110	3	immediately	immediately	RB
cord-293966-5c466xvz	110	4	adding	add	VBG
cord-293966-5c466xvz	110	5	0.5	0.5	CD
cord-293966-5c466xvz	110	6	-	-	SYM
cord-293966-5c466xvz	110	7	1	1	CD
cord-293966-5c466xvz	110	8	mL	ml	NN
cord-293966-5c466xvz	110	9	SOC	SOC	NNP
cord-293966-5c466xvz	110	10	to	to	IN
cord-293966-5c466xvz	110	11	the	the	DT
cord-293966-5c466xvz	110	12	cuvette	cuvette	NN
cord-293966-5c466xvz	110	13	,	,	,
cord-293966-5c466xvz	110	14	and	and	CC
cord-293966-5c466xvz	110	15	then	then	RB
cord-293966-5c466xvz	110	16	transfer	transfer	VB
cord-293966-5c466xvz	110	17	the	the	DT
cord-293966-5c466xvz	110	18	mixture	mixture	NN
cord-293966-5c466xvz	110	19	to	to	IN
cord-293966-5c466xvz	110	20	a	a	DT
cord-293966-5c466xvz	110	21	14	14	CD
cord-293966-5c466xvz	110	22	mL	ml	NN
cord-293966-5c466xvz	110	23	culture	culture	NN
cord-293966-5c466xvz	110	24	tube	tube	NN
cord-293966-5c466xvz	110	25	.	.	.
cord-293966-5c466xvz	111	1	Incubate	incubate	VB
cord-293966-5c466xvz	111	2	at	at	IN
cord-293966-5c466xvz	111	3	32	32	CD
cord-293966-5c466xvz	111	4	C	c	NN
cord-293966-5c466xvz	111	5	and	and	CC
cord-293966-5c466xvz	111	6	220	220	CD
cord-293966-5c466xvz	111	7	rpm	rpm	NN
cord-293966-5c466xvz	111	8	for	for	IN
cord-293966-5c466xvz	111	9	3	3	CD
cord-293966-5c466xvz	111	10	-	-	SYM
cord-293966-5c466xvz	111	11	5	5	CD
cord-293966-5c466xvz	111	12	h.	h.	NN
cord-293966-5c466xvz	112	1	This	this	DT
cord-293966-5c466xvz	112	2	is	be	VBZ
cord-293966-5c466xvz	112	3	when	when	WRB
cord-293966-5c466xvz	112	4	the	the	DT
cord-293966-5c466xvz	112	5	recombination	recombination	NN
cord-293966-5c466xvz	112	6	occurs	occur	VBZ
cord-293966-5c466xvz	113	1	(	(	-LRB-
cord-293966-5c466xvz	113	2	Fig	Fig	NNP
cord-293966-5c466xvz	113	3	.	.	.
cord-293966-5c466xvz	113	4	1d	1d	CD
cord-293966-5c466xvz	113	5	)	)	-RRB-
cord-293966-5c466xvz	114	1	Fig	Fig	NNP
cord-293966-5c466xvz	114	2	.	.	.
cord-293966-5c466xvz	115	1	3	3	LS
cord-293966-5c466xvz	115	2	Replica	Replica	NNP
cord-293966-5c466xvz	115	3	plate	plate	NN
cord-293966-5c466xvz	115	4	grids	grid	NNS
cord-293966-5c466xvz	115	5	.	.	.
cord-293966-5c466xvz	116	1	These	these	DT
cord-293966-5c466xvz	116	2	grids	grid	NNS
cord-293966-5c466xvz	116	3	allow	allow	VBP
cord-293966-5c466xvz	116	4	for	for	IN
cord-293966-5c466xvz	116	5	the	the	DT
cord-293966-5c466xvz	116	6	easy	easy	JJ
cord-293966-5c466xvz	116	7	identification	identification	NN
cord-293966-5c466xvz	116	8	of	of	IN
cord-293966-5c466xvz	116	9	identical	identical	JJ
cord-293966-5c466xvz	116	10	colonies	colony	NNS
cord-293966-5c466xvz	116	11	that	that	WDT
cord-293966-5c466xvz	116	12	have	have	VBP
cord-293966-5c466xvz	116	13	been	be	VBN
cord-293966-5c466xvz	116	14	plated	plate	VBN
cord-293966-5c466xvz	116	15	on	on	IN
cord-293966-5c466xvz	116	16	each	each	DT
cord-293966-5c466xvz	116	17	plate	plate	NN
cord-293966-5c466xvz	116	18	.	.	.
cord-293966-5c466xvz	117	1	Using	use	VBG
cord-293966-5c466xvz	117	2	a	a	DT
cord-293966-5c466xvz	117	3	toothpick	toothpick	NN
cord-293966-5c466xvz	117	4	,	,	,
cord-293966-5c466xvz	117	5	dot	dot	VB
cord-293966-5c466xvz	117	6	a	a	DT
cord-293966-5c466xvz	117	7	single	single	JJ
cord-293966-5c466xvz	117	8	colony	colony	NN
cord-293966-5c466xvz	117	9	in	in	IN
cord-293966-5c466xvz	117	10	the	the	DT
cord-293966-5c466xvz	117	11	same	same	JJ
cord-293966-5c466xvz	117	12	spot	spot	NN
cord-293966-5c466xvz	117	13	on	on	IN
cord-293966-5c466xvz	117	14	each	each	DT
cord-293966-5c466xvz	117	15	plate	plate	NN
cord-293966-5c466xvz	117	16	.	.	.
cord-293966-5c466xvz	118	1	For	for	IN
cord-293966-5c466xvz	118	2	both	both	DT
cord-293966-5c466xvz	118	3	positive	positive	JJ
cord-293966-5c466xvz	118	4	and	and	CC
cord-293966-5c466xvz	118	5	negative	negative	JJ
cord-293966-5c466xvz	118	6	selection	selection	NN
cord-293966-5c466xvz	118	7	,	,	,
cord-293966-5c466xvz	118	8	MERS	MERS	NNP
cord-293966-5c466xvz	118	9	-	-	HYPH
cord-293966-5c466xvz	118	10	BAC	BAC	NNP
cord-293966-5c466xvz	118	11	clones	clone	NNS
cord-293966-5c466xvz	118	12	that	that	WDT
cord-293966-5c466xvz	118	13	have	have	VBP
cord-293966-5c466xvz	118	14	successfully	successfully	RB
cord-293966-5c466xvz	118	15	undergone	undergo	VBN
cord-293966-5c466xvz	118	16	recombination	recombination	NN
cord-293966-5c466xvz	118	17	will	will	MD
cord-293966-5c466xvz	118	18	grow	grow	VB
cord-293966-5c466xvz	118	19	on	on	IN
cord-293966-5c466xvz	118	20	the	the	DT
cord-293966-5c466xvz	118	21	plate	plate	NN
cord-293966-5c466xvz	118	22	on	on	IN
cord-293966-5c466xvz	118	23	the	the	DT
cord-293966-5c466xvz	118	24	right	right	NN
cord-293966-5c466xvz	118	25	,	,	,
cord-293966-5c466xvz	118	26	but	but	CC
cord-293966-5c466xvz	118	27	not	not	RB
cord-293966-5c466xvz	118	28	on	on	IN
cord-293966-5c466xvz	118	29	the	the	DT
cord-293966-5c466xvz	118	30	plate	plate	NN
cord-293966-5c466xvz	118	31	on	on	IN
cord-293966-5c466xvz	118	32	the	the	DT
cord-293966-5c466xvz	118	33	left	left	JJ
cord-293966-5c466xvz	118	34	Day	Day	NNP
cord-293966-5c466xvz	118	35	4	4	CD
cord-293966-5c466xvz	118	36	3	3	CD
cord-293966-5c466xvz	118	37	.	.	.
cord-293966-5c466xvz	119	1	Following	follow	VBG
cord-293966-5c466xvz	119	2	o	o	NN
cord-293966-5c466xvz	119	3	/	/	SYM
cord-293966-5c466xvz	119	4	n	n	CC
cord-293966-5c466xvz	119	5	incubation	incubation	NN
cord-293966-5c466xvz	119	6	,	,	,
cord-293966-5c466xvz	119	7	create	create	VB
cord-293966-5c466xvz	119	8	a	a	DT
cord-293966-5c466xvz	119	9	freezer	freezer	NN
cord-293966-5c466xvz	119	10	stock	stock	NN
cord-293966-5c466xvz	119	11	of	of	IN
cord-293966-5c466xvz	119	12	the	the	DT
cord-293966-5c466xvz	119	13	bacteria	bacteria	NNS
cord-293966-5c466xvz	119	14	,	,	,
cord-293966-5c466xvz	119	15	then	then	RB
cord-293966-5c466xvz	119	16	purify	purify	VB
cord-293966-5c466xvz	119	17	the	the	DT
cord-293966-5c466xvz	119	18	BAC	BAC	NNP
cord-293966-5c466xvz	119	19	DNA	DNA	NNP
cord-293966-5c466xvz	119	20	using	use	VBG
cord-293966-5c466xvz	119	21	a	a	DT
cord-293966-5c466xvz	119	22	standard	standard	JJ
cord-293966-5c466xvz	119	23	miniprep	miniprep	NNP
cord-293966-5c466xvz	119	24	kit	kit	NN
cord-293966-5c466xvz	119	25	.	.	.
cord-293966-5c466xvz	120	1	Using	use	VBG
cord-293966-5c466xvz	120	2	1	1	CD
cord-293966-5c466xvz	120	3	μL	μL	NNS
cord-293966-5c466xvz	120	4	of	of	IN
cord-293966-5c466xvz	120	5	the	the	DT
cord-293966-5c466xvz	120	6	BAC	BAC	NNP
cord-293966-5c466xvz	120	7	DNA	DNA	NNP
cord-293966-5c466xvz	120	8	,	,	,
cord-293966-5c466xvz	120	9	use	use	VB
cord-293966-5c466xvz	120	10	the	the	DT
cord-293966-5c466xvz	120	11	external	external	JJ
cord-293966-5c466xvz	120	12	primers	primer	NNS
cord-293966-5c466xvz	120	13	located	locate	VBD
cord-293966-5c466xvz	120	14	100	100	CD
cord-293966-5c466xvz	120	15	-	-	SYM
cord-293966-5c466xvz	120	16	200	200	CD
cord-293966-5c466xvz	120	17	bp	bp	NNP
cord-293966-5c466xvz	120	18	outside	outside	IN
cord-293966-5c466xvz	120	19	the	the	DT
cord-293966-5c466xvz	120	20	region	region	NN
cord-293966-5c466xvz	120	21	of	of	IN
cord-293966-5c466xvz	120	22	homology	homology	NN
cord-293966-5c466xvz	120	23	you	-PRON-	PRP
cord-293966-5c466xvz	120	24	previously	previously	RB
cord-293966-5c466xvz	120	25	designed	design	VBN
cord-293966-5c466xvz	120	26	to	to	TO
cord-293966-5c466xvz	120	27	test	test	VB
cord-293966-5c466xvz	120	28	for	for	IN
cord-293966-5c466xvz	120	29	the	the	DT
cord-293966-5c466xvz	120	30	insertion	insertion	NN
cord-293966-5c466xvz	120	31	of	of	IN
cord-293966-5c466xvz	120	32	Kan	Kan	NNP
cord-293966-5c466xvz	120	33	r	r	NNP
cord-293966-5c466xvz	120	34	-I	-I	NNP
cord-293966-5c466xvz	120	35	-	-	:
cord-293966-5c466xvz	120	36	SceI	SceI	NNP
cord-293966-5c466xvz	120	37	by	by	IN
cord-293966-5c466xvz	120	38	PCR	PCR	NNP
cord-293966-5c466xvz	120	39	.	.	.
cord-293966-5c466xvz	121	1	If	if	IN
cord-293966-5c466xvz	121	2	the	the	DT
cord-293966-5c466xvz	121	3	insertion	insertion	NN
cord-293966-5c466xvz	121	4	was	be	VBD
cord-293966-5c466xvz	121	5	successful	successful	JJ
cord-293966-5c466xvz	121	6	,	,	,
cord-293966-5c466xvz	121	7	the	the	DT
cord-293966-5c466xvz	121	8	DNA	dna	NN
cord-293966-5c466xvz	121	9	band	band	NN
cord-293966-5c466xvz	121	10	from	from	IN
cord-293966-5c466xvz	121	11	the	the	DT
cord-293966-5c466xvz	121	12	PCR	PCR	NNP
cord-293966-5c466xvz	121	13	should	should	MD
cord-293966-5c466xvz	121	14	be~1	be~1	VB
cord-293966-5c466xvz	121	15	kb	kb	NNP
cord-293966-5c466xvz	121	16	larger	large	JJR
cord-293966-5c466xvz	121	17	than	than	IN
cord-293966-5c466xvz	121	18	the	the	DT
cord-293966-5c466xvz	121	19	band	band	NN
cord-293966-5c466xvz	121	20	from	from	IN
cord-293966-5c466xvz	121	21	the	the	DT
cord-293966-5c466xvz	121	22	MERS	MERS	NNP
cord-293966-5c466xvz	121	23	-	-	HYPH
cord-293966-5c466xvz	121	24	CoV	CoV	NNP
cord-293966-5c466xvz	121	25	wild	wild	JJ
cord-293966-5c466xvz	121	26	-	-	HYPH
cord-293966-5c466xvz	121	27	type	type	NN
cord-293966-5c466xvz	121	28	BAC	BAC	NNP
cord-293966-5c466xvz	121	29	(	(	-LRB-
cord-293966-5c466xvz	121	30	see	see	VB
cord-293966-5c466xvz	121	31	Note	Note	NNP
cord-293966-5c466xvz	121	32	12	12	CD
cord-293966-5c466xvz	121	33	)	)	-RRB-
cord-293966-5c466xvz	121	34	.	.	.
cord-293966-5c466xvz	122	1	To	to	TO
cord-293966-5c466xvz	122	2	speed	speed	VB
cord-293966-5c466xvz	122	3	this	this	DT
cord-293966-5c466xvz	122	4	process	process	NN
cord-293966-5c466xvz	122	5	up	up	RP
cord-293966-5c466xvz	122	6	,	,	,
cord-293966-5c466xvz	122	7	colonies	colony	NNS
cord-293966-5c466xvz	122	8	may	may	MD
cord-293966-5c466xvz	122	9	be	be	VB
cord-293966-5c466xvz	122	10	collected	collect	VBN
cord-293966-5c466xvz	122	11	off	off	IN
cord-293966-5c466xvz	122	12	the	the	DT
cord-293966-5c466xvz	122	13	plate	plate	NN
cord-293966-5c466xvz	122	14	on	on	IN
cord-293966-5c466xvz	122	15	day	day	NN
cord-293966-5c466xvz	122	16	3	3	CD
cord-293966-5c466xvz	122	17	and	and	CC
cord-293966-5c466xvz	122	18	directly	directly	RB
cord-293966-5c466xvz	122	19	tested	test	VBN
cord-293966-5c466xvz	122	20	by	by	IN
cord-293966-5c466xvz	122	21	PCR	PCR	NNP
cord-293966-5c466xvz	122	22	.	.	.
cord-293966-5c466xvz	123	1	Colonies	colony	NNS
cord-293966-5c466xvz	123	2	that	that	WDT
cord-293966-5c466xvz	123	3	pass	pass	VBP
cord-293966-5c466xvz	123	4	the	the	DT
cord-293966-5c466xvz	123	5	PCR	PCR	NNP
cord-293966-5c466xvz	123	6	screen	screen	NN
cord-293966-5c466xvz	123	7	can	can	MD
cord-293966-5c466xvz	123	8	then	then	RB
cord-293966-5c466xvz	123	9	progress	progress	VB
cord-293966-5c466xvz	123	10	to	to	IN
cord-293966-5c466xvz	123	11	the	the	DT
cord-293966-5c466xvz	123	12	negative	negative	JJ
cord-293966-5c466xvz	123	13	selection	selection	NN
cord-293966-5c466xvz	123	14	protocol	protocol	NN
cord-293966-5c466xvz	123	15	(	(	-LRB-
cord-293966-5c466xvz	123	16	3.5	3.5	CD
cord-293966-5c466xvz	123	17	)	)	-RRB-
cord-293966-5c466xvz	123	18	(	(	-LRB-
cord-293966-5c466xvz	123	19	see	see	VB
cord-293966-5c466xvz	123	20	Note	Note	NNP
cord-293966-5c466xvz	123	21	13	13	CD
cord-293966-5c466xvz	123	22	)	)	-RRB-
cord-293966-5c466xvz	123	23	.	.	.
cord-293966-5c466xvz	124	1	3	3	LS
cord-293966-5c466xvz	124	2	.	.	.
cord-293966-5c466xvz	125	1	After	after	IN
cord-293966-5c466xvz	125	2	2	2	CD
cord-293966-5c466xvz	125	3	h	h	NN
cord-293966-5c466xvz	125	4	,	,	,
cord-293966-5c466xvz	125	5	add	add	VB
cord-293966-5c466xvz	125	6	2	2	CD
cord-293966-5c466xvz	125	7	mL	mL	NNS
cord-293966-5c466xvz	125	8	of	of	IN
cord-293966-5c466xvz	125	9	warm	warm	JJ
cord-293966-5c466xvz	125	10	LB	LB	NNP
cord-293966-5c466xvz	125	11	-	-	HYPH
cord-293966-5c466xvz	125	12	cml	cml	NNP
cord-293966-5c466xvz	125	13	with	with	IN
cord-293966-5c466xvz	125	14	2	2	CD
cord-293966-5c466xvz	125	15	%	%	NN
cord-293966-5c466xvz	125	16	arabinose	arabinose	JJ
cord-293966-5c466xvz	125	17	to	to	IN
cord-293966-5c466xvz	125	18	the	the	DT
cord-293966-5c466xvz	125	19	culture	culture	NN
cord-293966-5c466xvz	125	20	tube	tube	NN
cord-293966-5c466xvz	125	21	for	for	IN
cord-293966-5c466xvz	125	22	a	a	DT
cord-293966-5c466xvz	125	23	final	final	JJ
cord-293966-5c466xvz	125	24	arabinose	arabinose	JJ
cord-293966-5c466xvz	125	25	concentration	concentration	NN
cord-293966-5c466xvz	125	26	of	of	IN
cord-293966-5c466xvz	125	27	1	1	CD
cord-293966-5c466xvz	125	28	%	%	NN
cord-293966-5c466xvz	126	1	(	(	-LRB-
cord-293966-5c466xvz	126	2	Fig	Fig	NNP
cord-293966-5c466xvz	126	3	.	.	NNP
cord-293966-5c466xvz	126	4	1	1	CD
cord-293966-5c466xvz	126	5	)	)	-RRB-
cord-293966-5c466xvz	126	6	.	.	.
cord-293966-5c466xvz	127	1	Incubate	incubate	VB
cord-293966-5c466xvz	127	2	at	at	IN
cord-293966-5c466xvz	127	3	32	32	CD
cord-293966-5c466xvz	127	4	C	c	NN
cord-293966-5c466xvz	127	5	and	and	CC
cord-293966-5c466xvz	127	6	220	220	CD
cord-293966-5c466xvz	127	7	rpm	rpm	NN
cord-293966-5c466xvz	127	8	for	for	IN
cord-293966-5c466xvz	127	9	2	2	CD
cord-293966-5c466xvz	127	10	h.	h.	NNP
cord-293966-5c466xvz	127	11	Warm	warm	JJ
cord-293966-5c466xvz	127	12	water	water	NN
cord-293966-5c466xvz	127	13	bath	bath	NN
cord-293966-5c466xvz	127	14	shaker	shaker	NN
cord-293966-5c466xvz	127	15	to	to	IN
cord-293966-5c466xvz	127	16	42	42	CD
cord-293966-5c466xvz	127	17	C.	C.	NNP
cord-293966-5c466xvz	127	18	4	4	CD
cord-293966-5c466xvz	127	19	.	.	.
cord-293966-5c466xvz	128	1	Transfer	transfer	VB
cord-293966-5c466xvz	128	2	the	the	DT
cord-293966-5c466xvz	128	3	culture	culture	NN
cord-293966-5c466xvz	128	4	tubes	tube	NNS
cord-293966-5c466xvz	128	5	to	to	IN
cord-293966-5c466xvz	128	6	the	the	DT
cord-293966-5c466xvz	128	7	water	water	NN
cord-293966-5c466xvz	128	8	bath	bath	NN
cord-293966-5c466xvz	128	9	shaker	shaker	NN
cord-293966-5c466xvz	128	10	at	at	IN
cord-293966-5c466xvz	128	11	42	42	CD
cord-293966-5c466xvz	128	12	C	c	NN
cord-293966-5c466xvz	128	13	and	and	CC
cord-293966-5c466xvz	128	14	200	200	CD
cord-293966-5c466xvz	128	15	rpm	rpm	NN
cord-293966-5c466xvz	128	16	and	and	CC
cord-293966-5c466xvz	128	17	incubate	incubate	VB
cord-293966-5c466xvz	128	18	for	for	IN
cord-293966-5c466xvz	128	19	30	30	CD
cord-293966-5c466xvz	128	20	min	min	NN
cord-293966-5c466xvz	128	21	(	(	-LRB-
cord-293966-5c466xvz	128	22	Fig	Fig	NNP
cord-293966-5c466xvz	128	23	.	.	.
cord-293966-5c466xvz	128	24	1f	1f	CD
cord-293966-5c466xvz	128	25	)	)	-RRB-
cord-293966-5c466xvz	128	26	.	.	.
cord-293966-5c466xvz	129	1	5	5	LS
cord-293966-5c466xvz	129	2	.	.	.
cord-293966-5c466xvz	130	1	Transfer	transfer	VB
cord-293966-5c466xvz	130	2	the	the	DT
cord-293966-5c466xvz	130	3	culture	culture	NN
cord-293966-5c466xvz	130	4	tubes	tube	NNS
cord-293966-5c466xvz	130	5	back	back	RB
cord-293966-5c466xvz	130	6	to	to	IN
cord-293966-5c466xvz	130	7	32	32	CD
cord-293966-5c466xvz	130	8	C	c	NN
cord-293966-5c466xvz	130	9	and	and	CC
cord-293966-5c466xvz	130	10	incubate	incubate	VB
cord-293966-5c466xvz	130	11	for	for	IN
cord-293966-5c466xvz	130	12	3	3	CD
cord-293966-5c466xvz	130	13	-	-	SYM
cord-293966-5c466xvz	130	14	4	4	CD
cord-293966-5c466xvz	130	15	h	h	NN
cord-293966-5c466xvz	130	16	(	(	-LRB-
cord-293966-5c466xvz	130	17	Fig	Fig	NNP
cord-293966-5c466xvz	130	18	.	.	.
cord-293966-5c466xvz	130	19	1	1	CD
cord-293966-5c466xvz	130	20	g	g	NN
cord-293966-5c466xvz	130	21	)	)	-RRB-
cord-293966-5c466xvz	130	22	.	.	.
cord-293966-5c466xvz	131	1	6	6	LS
cord-293966-5c466xvz	131	2	.	.	.
cord-293966-5c466xvz	132	1	After	after	IN
cord-293966-5c466xvz	132	2	the	the	DT
cord-293966-5c466xvz	132	3	incubation	incubation	NN
cord-293966-5c466xvz	132	4	,	,	,
cord-293966-5c466xvz	132	5	perform	perform	VB
cord-293966-5c466xvz	132	6	tenfold	tenfold	RB
cord-293966-5c466xvz	132	7	serial	serial	JJ
cord-293966-5c466xvz	132	8	dilutions	dilution	NNS
cord-293966-5c466xvz	132	9	of	of	IN
cord-293966-5c466xvz	132	10	the	the	DT
cord-293966-5c466xvz	132	11	bacteria	bacteria	NNS
cord-293966-5c466xvz	132	12	in	in	IN
cord-293966-5c466xvz	132	13	LB	LB	NNP
cord-293966-5c466xvz	132	14	.	.	.
cord-293966-5c466xvz	133	1	Plate	plate	NN
cord-293966-5c466xvz	133	2	100	100	CD
cord-293966-5c466xvz	133	3	μL	μL	NNS
cord-293966-5c466xvz	133	4	of	of	IN
cord-293966-5c466xvz	133	5	10	10	CD
cord-293966-5c466xvz	133	6	À4	à4	CD
cord-293966-5c466xvz	133	7	and	and	CC
cord-293966-5c466xvz	133	8	10	10	CD
cord-293966-5c466xvz	133	9	À5	À5	NNP
cord-293966-5c466xvz	133	10	dilutions	dilution	NNS
cord-293966-5c466xvz	133	11	of	of	IN
cord-293966-5c466xvz	133	12	original	original	JJ
cord-293966-5c466xvz	133	13	culture	culture	NN
cord-293966-5c466xvz	133	14	on	on	IN
cord-293966-5c466xvz	133	15	pre	pre	JJ
cord-293966-5c466xvz	133	16	-	-	JJ
cord-293966-5c466xvz	133	17	warmed	warm	VBN
cord-293966-5c466xvz	133	18	LB	LB	NNP
cord-293966-5c466xvz	133	19	-	-	HYPH
cord-293966-5c466xvz	133	20	cml	cml	NNP
cord-293966-5c466xvz	133	21	plates	plate	NNS
cord-293966-5c466xvz	133	22	containing	contain	VBG
cord-293966-5c466xvz	133	23	1	1	CD
cord-293966-5c466xvz	133	24	%	%	NN
cord-293966-5c466xvz	133	25	arabinose	arabinose	NN
cord-293966-5c466xvz	133	26	.	.	.
cord-293966-5c466xvz	134	1	Incubate	incubate	VB
cord-293966-5c466xvz	134	2	the	the	DT
cord-293966-5c466xvz	134	3	plates	plate	NNS
cord-293966-5c466xvz	134	4	at	at	IN
cord-293966-5c466xvz	134	5	30	30	CD
cord-293966-5c466xvz	134	6	-	-	SYM
cord-293966-5c466xvz	134	7	32	32	CD
cord-293966-5c466xvz	134	8	C.	C.	NNP
cord-293966-5c466xvz	134	9	Day	Day	NNP
cord-293966-5c466xvz	134	10	3	3	CD
cord-293966-5c466xvz	134	11	7	7	CD
cord-293966-5c466xvz	134	12	.	.	.
cord-293966-5c466xvz	135	1	Pick	pick	VB
cord-293966-5c466xvz	135	2	50	50	CD
cord-293966-5c466xvz	135	3	colonies	colony	NNS
cord-293966-5c466xvz	135	4	and	and	CC
cord-293966-5c466xvz	135	5	replica	replica	NN
cord-293966-5c466xvz	135	6	plate	plate	NN
cord-293966-5c466xvz	135	7	on	on	IN
cord-293966-5c466xvz	135	8	LB	LB	NNP
cord-293966-5c466xvz	135	9	-	-	HYPH
cord-293966-5c466xvz	135	10	cml	cml	NNP
cord-293966-5c466xvz	135	11	/	/	SYM
cord-293966-5c466xvz	135	12	kan	kan	NNP
cord-293966-5c466xvz	135	13	and	and	CC
cord-293966-5c466xvz	135	14	LB	LB	NNP
cord-293966-5c466xvz	135	15	-	-	HYPH
cord-293966-5c466xvz	135	16	cml	cml	NNP
cord-293966-5c466xvz	135	17	plates	plate	NNS
cord-293966-5c466xvz	135	18	.	.	.
cord-293966-5c466xvz	136	1	Colonies	colony	NNS
cord-293966-5c466xvz	136	2	that	that	WDT
cord-293966-5c466xvz	136	3	underwent	undergo	VBD
cord-293966-5c466xvz	136	4	correct	correct	JJ
cord-293966-5c466xvz	136	5	recombination	recombination	NN
cord-293966-5c466xvz	136	6	should	should	MD
cord-293966-5c466xvz	136	7	grow	grow	VB
cord-293966-5c466xvz	136	8	on	on	IN
cord-293966-5c466xvz	136	9	LB	LB	NNP
cord-293966-5c466xvz	136	10	-	-	HYPH
cord-293966-5c466xvz	136	11	cml	cml	NNP
cord-293966-5c466xvz	136	12	but	but	CC
cord-293966-5c466xvz	136	13	not	not	RB
cord-293966-5c466xvz	136	14	on	on	IN
cord-293966-5c466xvz	136	15	LB	LB	NNP
cord-293966-5c466xvz	136	16	-	-	HYPH
cord-293966-5c466xvz	136	17	cml	cml	NNP
cord-293966-5c466xvz	136	18	/	/	SYM
cord-293966-5c466xvz	136	19	kan	kan	NNP
cord-293966-5c466xvz	136	20	.	.	.
cord-293966-5c466xvz	137	1	Incubate	incubate	VB
cord-293966-5c466xvz	137	2	o	o	NN
cord-293966-5c466xvz	137	3	/	/	SYM
cord-293966-5c466xvz	137	4	n	n	CC
cord-293966-5c466xvz	137	5	at	at	IN
cord-293966-5c466xvz	137	6	32	32	CD
cord-293966-5c466xvz	137	7	C.	C.	NNP
cord-293966-5c466xvz	137	8	Efficiency	Efficiency	NNP
cord-293966-5c466xvz	137	9	is	be	VBZ
cord-293966-5c466xvz	137	10	generally	generally	RB
cord-293966-5c466xvz	137	11	anywhere	anywhere	RB
cord-293966-5c466xvz	137	12	from	from	IN
cord-293966-5c466xvz	137	13	5	5	CD
cord-293966-5c466xvz	137	14	to	to	TO
cord-293966-5c466xvz	137	15	50	50	CD
cord-293966-5c466xvz	137	16	%	%	NN
cord-293966-5c466xvz	137	17	(	(	-LRB-
cord-293966-5c466xvz	137	18	see	see	VB
cord-293966-5c466xvz	137	19	Note	Note	NNP
cord-293966-5c466xvz	137	20	14	14	CD
cord-293966-5c466xvz	137	21	)	)	-RRB-
cord-293966-5c466xvz	137	22	.	.	.
cord-293966-5c466xvz	138	1	Day	day	NN
cord-293966-5c466xvz	138	2	4	4	CD
cord-293966-5c466xvz	138	3	8	8	CD
cord-293966-5c466xvz	138	4	.	.	.
cord-293966-5c466xvz	139	1	Pick	pick	VB
cord-293966-5c466xvz	139	2	3	3	CD
cord-293966-5c466xvz	140	1	separate	separate	JJ
cord-293966-5c466xvz	140	2	cml	cml	NNP
cord-293966-5c466xvz	140	3	+	+	CC
cord-293966-5c466xvz	140	4	kan	kan	NNP
cord-293966-5c466xvz	140	5	À	À	NNP
cord-293966-5c466xvz	140	6	colonies	colony	NNS
cord-293966-5c466xvz	140	7	and	and	CC
cord-293966-5c466xvz	140	8	culture	culture	NN
cord-293966-5c466xvz	140	9	each	each	DT
cord-293966-5c466xvz	140	10	one	one	CD
cord-293966-5c466xvz	140	11	in	in	IN
cord-293966-5c466xvz	140	12	100	100	CD
cord-293966-5c466xvz	140	13	mL	ml	NN
cord-293966-5c466xvz	140	14	LB	LB	NNP
cord-293966-5c466xvz	140	15	-	-	HYPH
cord-293966-5c466xvz	140	16	cml	cml	NNP
cord-293966-5c466xvz	140	17	at	at	IN
cord-293966-5c466xvz	140	18	32	32	CD
cord-293966-5c466xvz	140	19	C	c	NN
cord-293966-5c466xvz	140	20	and	and	CC
cord-293966-5c466xvz	140	21	220	220	CD
cord-293966-5c466xvz	140	22	rpm	rpm	NN
cord-293966-5c466xvz	140	23	o	o	NN
cord-293966-5c466xvz	140	24	/	/	SYM
cord-293966-5c466xvz	140	25	n	n	NN
cord-293966-5c466xvz	140	26	.	.	NNP
cord-293966-5c466xvz	140	27	2	2	CD
cord-293966-5c466xvz	140	28	.	.	.
cord-293966-5c466xvz	141	1	All	all	DT
cord-293966-5c466xvz	141	2	procedures	procedure	NNS
cord-293966-5c466xvz	141	3	from	from	IN
cord-293966-5c466xvz	141	4	here	here	RB
cord-293966-5c466xvz	141	5	involve	involve	VBP
cord-293966-5c466xvz	141	6	working	work	VBG
cord-293966-5c466xvz	141	7	with	with	IN
cord-293966-5c466xvz	141	8	MERS	MERS	NNP
cord-293966-5c466xvz	141	9	-	-	HYPH
cord-293966-5c466xvz	141	10	CoV	CoV	NNP
cord-293966-5c466xvz	141	11	which	which	WDT
cord-293966-5c466xvz	141	12	requires	require	VBZ
cord-293966-5c466xvz	141	13	a	a	DT
cord-293966-5c466xvz	141	14	BSL-3	BSL-3	NNP
cord-293966-5c466xvz	141	15	containment	containment	NN
cord-293966-5c466xvz	141	16	laboratory	laboratory	NN
cord-293966-5c466xvz	141	17	.	.	.
cord-293966-5c466xvz	142	1	3	3	LS
cord-293966-5c466xvz	142	2	.	.	.
cord-293966-5c466xvz	143	1	Replace	replace	VB
cord-293966-5c466xvz	143	2	medium	medium	NN
cord-293966-5c466xvz	143	3	from	from	IN
cord-293966-5c466xvz	143	4	cells	cell	NNS
cord-293966-5c466xvz	143	5	with	with	IN
cord-293966-5c466xvz	143	6	2	2	CD
cord-293966-5c466xvz	143	7	mL	ml	NN
cord-293966-5c466xvz	143	8	DMEM	dmem	NN
cord-293966-5c466xvz	143	9	+10	+10	CD
cord-293966-5c466xvz	143	10	%	%	NN
cord-293966-5c466xvz	143	11	FBS	FBS	NNP
cord-293966-5c466xvz	143	12	immediately	immediately	RB
cord-293966-5c466xvz	143	13	before	before	IN
cord-293966-5c466xvz	143	14	proceeding	proceed	VBG
cord-293966-5c466xvz	143	15	to	to	IN
cord-293966-5c466xvz	143	16	step4	step4	NN
cord-293966-5c466xvz	143	17	.	.	.
cord-293966-5c466xvz	144	1	6	6	LS
cord-293966-5c466xvz	144	2	.	.	.
cord-293966-5c466xvz	145	1	Cytopathic	cytopathic	JJ
cord-293966-5c466xvz	145	2	effect	effect	NN
cord-293966-5c466xvz	145	3	(	(	-LRB-
cord-293966-5c466xvz	145	4	CPE	CPE	NNP
cord-293966-5c466xvz	145	5	)	)	-RRB-
cord-293966-5c466xvz	145	6	will	will	MD
cord-293966-5c466xvz	145	7	start	start	VB
cord-293966-5c466xvz	145	8	being	be	VBG
cord-293966-5c466xvz	145	9	visible	visible	JJ
cord-293966-5c466xvz	145	10	at	at	IN
cord-293966-5c466xvz	145	11	3	3	CD
cord-293966-5c466xvz	145	12	-	-	SYM
cord-293966-5c466xvz	145	13	4	4	CD
cord-293966-5c466xvz	145	14	days	day	NNS
cord-293966-5c466xvz	145	15	after	after	IN
cord-293966-5c466xvz	145	16	transfection	transfection	NN
cord-293966-5c466xvz	145	17	.	.	.
cord-293966-5c466xvz	146	1	Collect	collect	VB
cord-293966-5c466xvz	146	2	cells	cell	NNS
cord-293966-5c466xvz	146	3	and	and	CC
cord-293966-5c466xvz	146	4	supernatant	supernatant	JJ
cord-293966-5c466xvz	146	5	when	when	WRB
cord-293966-5c466xvz	146	6	>	>	XX
cord-293966-5c466xvz	146	7	50	50	CD
cord-293966-5c466xvz	146	8	%	%	NN
cord-293966-5c466xvz	146	9	of	of	IN
cord-293966-5c466xvz	146	10	well	well	NN
cord-293966-5c466xvz	146	11	has	have	VBZ
cord-293966-5c466xvz	146	12	CPE	CPE	NNP
cord-293966-5c466xvz	146	13	(	(	-LRB-
cord-293966-5c466xvz	146	14	the	the	DT
cord-293966-5c466xvz	146	15	more	more	RBR
cord-293966-5c466xvz	146	16	the	the	DT
cord-293966-5c466xvz	146	17	better	well	JJR
cord-293966-5c466xvz	146	18	)	)	-RRB-
cord-293966-5c466xvz	146	19	.	.	.
cord-293966-5c466xvz	147	1	To	to	TO
cord-293966-5c466xvz	147	2	collect	collect	VB
cord-293966-5c466xvz	147	3	,	,	,
cord-293966-5c466xvz	147	4	scrape	scrape	VB
cord-293966-5c466xvz	147	5	any	any	DT
cord-293966-5c466xvz	147	6	remaining	remain	VBG
cord-293966-5c466xvz	147	7	cells	cell	NNS
cord-293966-5c466xvz	147	8	off	off	IN
cord-293966-5c466xvz	147	9	the	the	DT
cord-293966-5c466xvz	147	10	well	well	NN
cord-293966-5c466xvz	147	11	with	with	IN
cord-293966-5c466xvz	147	12	a	a	DT
cord-293966-5c466xvz	147	13	pipette	pipette	NN
cord-293966-5c466xvz	147	14	tip	tip	NN
cord-293966-5c466xvz	147	15	or	or	CC
cord-293966-5c466xvz	147	16	cell	cell	NN
cord-293966-5c466xvz	147	17	scraper	scraper	NN
cord-293966-5c466xvz	147	18	and	and	CC
cord-293966-5c466xvz	147	19	transfer	transfer	VB
cord-293966-5c466xvz	147	20	the	the	DT
cord-293966-5c466xvz	147	21	media	medium	NNS
cord-293966-5c466xvz	147	22	and	and	CC
cord-293966-5c466xvz	147	23	cell	cell	NN
cord-293966-5c466xvz	147	24	debris	debris	NN
cord-293966-5c466xvz	147	25	in	in	IN
cord-293966-5c466xvz	147	26	a	a	DT
cord-293966-5c466xvz	147	27	2	2	CD
cord-293966-5c466xvz	147	28	mL	ml	NN
cord-293966-5c466xvz	147	29	microcentrifuge	microcentrifuge	NN
cord-293966-5c466xvz	147	30	tube	tube	NN
cord-293966-5c466xvz	147	31	and	and	CC
cord-293966-5c466xvz	147	32	freeze	freeze	NN
cord-293966-5c466xvz	147	33	-	-	HYPH
cord-293966-5c466xvz	147	34	thaw	thaw	VB
cord-293966-5c466xvz	147	35	the	the	DT
cord-293966-5c466xvz	147	36	sample	sample	NN
cord-293966-5c466xvz	147	37	.	.	.
cord-293966-5c466xvz	148	1	Then	then	RB
cord-293966-5c466xvz	148	2	,	,	,
cord-293966-5c466xvz	148	3	centrifuge	centrifuge	VB
cord-293966-5c466xvz	148	4	the	the	DT
cord-293966-5c466xvz	148	5	sample	sample	NN
cord-293966-5c466xvz	149	1	at~5000	at~5000	NFP
cord-293966-5c466xvz	149	2	Â	â	NN
cord-293966-5c466xvz	149	3	g	g	NN
cord-293966-5c466xvz	149	4	to	to	TO
cord-293966-5c466xvz	149	5	spin	spin	VB
cord-293966-5c466xvz	149	6	out	out	RP
cord-293966-5c466xvz	149	7	the	the	DT
cord-293966-5c466xvz	149	8	cell	cell	NN
cord-293966-5c466xvz	149	9	debris	debris	NN
cord-293966-5c466xvz	149	10	and	and	CC
cord-293966-5c466xvz	149	11	transfer	transfer	VB
cord-293966-5c466xvz	149	12	the	the	DT
cord-293966-5c466xvz	149	13	supernatant	supernatant	NN
cord-293966-5c466xvz	149	14	to	to	IN
cord-293966-5c466xvz	149	15	a	a	DT
cord-293966-5c466xvz	149	16	new	new	JJ
cord-293966-5c466xvz	149	17	tube	tube	NN
cord-293966-5c466xvz	149	18	.	.	.
cord-293966-5c466xvz	150	1	We	-PRON-	PRP
cord-293966-5c466xvz	150	2	term	term	VBP
cord-293966-5c466xvz	150	3	this	this	DT
cord-293966-5c466xvz	150	4	passage	passage	NN
cord-293966-5c466xvz	150	5	0	0	NFP
cord-293966-5c466xvz	151	1	(	(	-LRB-
cord-293966-5c466xvz	151	2	P0	p0	NN
cord-293966-5c466xvz	151	3	)	)	-RRB-
cord-293966-5c466xvz	151	4	virus	virus	NN
cord-293966-5c466xvz	151	5	.	.	.
cord-293966-5c466xvz	152	1	3	3	LS
cord-293966-5c466xvz	152	2	.	.	.
cord-293966-5c466xvz	153	1	For	for	IN
cord-293966-5c466xvz	153	2	creating	create	VBG
cord-293966-5c466xvz	153	3	long	long	JJ
cord-293966-5c466xvz	153	4	oligos	oligo	NNS
cord-293966-5c466xvz	153	5	,	,	,
cord-293966-5c466xvz	153	6	we	-PRON-	PRP
cord-293966-5c466xvz	153	7	prefer	prefer	VBP
cord-293966-5c466xvz	153	8	Invitrogen	Invitrogen	NNP
cord-293966-5c466xvz	153	9	as	as	IN
cord-293966-5c466xvz	153	10	a	a	DT
cord-293966-5c466xvz	153	11	supplier	supplier	NN
cord-293966-5c466xvz	153	12	,	,	,
cord-293966-5c466xvz	153	13	as	as	IN
cord-293966-5c466xvz	153	14	they	-PRON-	PRP
cord-293966-5c466xvz	153	15	can	can	MD
cord-293966-5c466xvz	153	16	provide	provide	VB
cord-293966-5c466xvz	153	17	oligos	oligo	NNS
cord-293966-5c466xvz	153	18	up	up	IN
cord-293966-5c466xvz	153	19	to	to	TO
cord-293966-5c466xvz	153	20	100	100	CD
cord-293966-5c466xvz	153	21	nt	nt	RB
cord-293966-5c466xvz	153	22	at	at	IN
cord-293966-5c466xvz	153	23	their	-PRON-	PRP$
cord-293966-5c466xvz	153	24	standard	standard	JJ
cord-293966-5c466xvz	153	25	price	price	NN
cord-293966-5c466xvz	153	26	per	per	IN
cord-293966-5c466xvz	153	27	base	base	NN
cord-293966-5c466xvz	153	28	.	.	.
cord-293966-5c466xvz	154	1	Many	many	JJ
cord-293966-5c466xvz	154	2	companies	company	NNS
cord-293966-5c466xvz	154	3	do	do	VBP
cord-293966-5c466xvz	154	4	not	not	RB
cord-293966-5c466xvz	154	5	make	make	VB
cord-293966-5c466xvz	154	6	oligos	oligo	NNS
cord-293966-5c466xvz	154	7	longer	long	RBR
cord-293966-5c466xvz	154	8	than	than	IN
cord-293966-5c466xvz	154	9	60	60	CD
cord-293966-5c466xvz	154	10	nt	nt	RB
cord-293966-5c466xvz	154	11	at	at	IN
cord-293966-5c466xvz	154	12	their	-PRON-	PRP$
cord-293966-5c466xvz	154	13	standard	standard	JJ
cord-293966-5c466xvz	154	14	rates	rate	NNS
cord-293966-5c466xvz	154	15	,	,	,
cord-293966-5c466xvz	154	16	and	and	CC
cord-293966-5c466xvz	154	17	thus	thus	RB
cord-293966-5c466xvz	154	18	will	will	MD
cord-293966-5c466xvz	154	19	charge	charge	VB
cord-293966-5c466xvz	154	20	a	a	DT
cord-293966-5c466xvz	154	21	significant	significant	JJ
cord-293966-5c466xvz	154	22	amount	amount	NN
cord-293966-5c466xvz	154	23	more	more	JJR
cord-293966-5c466xvz	154	24	for	for	IN
cord-293966-5c466xvz	154	25	the	the	DT
cord-293966-5c466xvz	154	26	80	80	CD
cord-293966-5c466xvz	154	27	-	-	SYM
cord-293966-5c466xvz	154	28	90	90	CD
cord-293966-5c466xvz	154	29	nt	nt	NN
cord-293966-5c466xvz	154	30	oligos	oligo	NNS
cord-293966-5c466xvz	154	31	required	require	VBN
cord-293966-5c466xvz	154	32	for	for	IN
cord-293966-5c466xvz	154	33	this	this	DT
cord-293966-5c466xvz	154	34	protocol	protocol	NN
cord-293966-5c466xvz	154	35	.	.	.
cord-293966-5c466xvz	155	1	4	4	LS
cord-293966-5c466xvz	155	2	.	.	.
cord-293966-5c466xvz	156	1	While	while	IN
cord-293966-5c466xvz	156	2	many	many	JJ
cord-293966-5c466xvz	156	3	companies	company	NNS
cord-293966-5c466xvz	156	4	sell	sell	VBP
cord-293966-5c466xvz	156	5	BAC	BAC	NNP
cord-293966-5c466xvz	156	6	-	-	HYPH
cord-293966-5c466xvz	156	7	prep	prep	NN
cord-293966-5c466xvz	156	8	kits	kit	NNS
cord-293966-5c466xvz	156	9	which	which	WDT
cord-293966-5c466xvz	156	10	will	will	MD
cord-293966-5c466xvz	156	11	work	work	VB
cord-293966-5c466xvz	156	12	for	for	IN
cord-293966-5c466xvz	156	13	these	these	DT
cord-293966-5c466xvz	156	14	purposes	purpose	NNS
cord-293966-5c466xvz	156	15	,	,	,
cord-293966-5c466xvz	156	16	these	these	DT
cord-293966-5c466xvz	156	17	columns	column	NNS
cord-293966-5c466xvz	156	18	are	be	VBP
cord-293966-5c466xvz	156	19	often	often	RB
cord-293966-5c466xvz	156	20	at	at	IN
cord-293966-5c466xvz	156	21	least	least	JJS
cord-293966-5c466xvz	156	22	3Â	3â	CD
cord-293966-5c466xvz	156	23	the	the	DT
cord-293966-5c466xvz	156	24	cost	cost	NN
cord-293966-5c466xvz	156	25	of	of	IN
cord-293966-5c466xvz	156	26	the	the	DT
cord-293966-5c466xvz	156	27	Nucleobond	Nucleobond	NNP
cord-293966-5c466xvz	156	28	Xtra	Xtra	NNP
cord-293966-5c466xvz	156	29	Midi	Midi	NNP
cord-293966-5c466xvz	156	30	Kit	kit	NN
cord-293966-5c466xvz	156	31	.	.	.
cord-293966-5c466xvz	157	1	We	-PRON-	PRP
cord-293966-5c466xvz	157	2	have	have	VBP
cord-293966-5c466xvz	157	3	tested	test	VBN
cord-293966-5c466xvz	157	4	the	the	DT
cord-293966-5c466xvz	157	5	Xtra	Xtra	NNP
cord-293966-5c466xvz	157	6	Midi	Midi	NNP
cord-293966-5c466xvz	157	7	Kit	kit	NN
cord-293966-5c466xvz	157	8	side	side	NN
cord-293966-5c466xvz	157	9	by	by	IN
cord-293966-5c466xvz	157	10	side	side	NN
cord-293966-5c466xvz	157	11	with	with	IN
cord-293966-5c466xvz	157	12	a	a	DT
cord-293966-5c466xvz	157	13	BAC	BAC	NNP
cord-293966-5c466xvz	157	14	-	-	HYPH
cord-293966-5c466xvz	157	15	prep	prep	NN
cord-293966-5c466xvz	157	16	kit	kit	NN
cord-293966-5c466xvz	157	17	and	and	CC
cord-293966-5c466xvz	157	18	found	find	VBD
cord-293966-5c466xvz	157	19	little	little	JJ
cord-293966-5c466xvz	157	20	to	to	IN
cord-293966-5c466xvz	157	21	no	no	DT
cord-293966-5c466xvz	157	22	difference	difference	NN
cord-293966-5c466xvz	157	23	in	in	IN
cord-293966-5c466xvz	157	24	CoV	CoV	NNP
cord-293966-5c466xvz	157	25	BAC	BAC	NNP
cord-293966-5c466xvz	157	26	DNA	DNA	NNP
cord-293966-5c466xvz	157	27	yield	yield	NN
cord-293966-5c466xvz	157	28	.	.	.
cord-293966-5c466xvz	158	1	5	5	LS
cord-293966-5c466xvz	158	2	.	.	.
cord-293966-5c466xvz	159	1	For	for	IN
cord-293966-5c466xvz	159	2	purifying	purify	VBG
cord-293966-5c466xvz	159	3	PCR	PCR	NNP
cord-293966-5c466xvz	159	4	products	product	NNS
cord-293966-5c466xvz	159	5	,	,	,
cord-293966-5c466xvz	159	6	we	-PRON-	PRP
cord-293966-5c466xvz	159	7	prefer	prefer	VBP
cord-293966-5c466xvz	159	8	the	the	DT
cord-293966-5c466xvz	159	9	Invitrogen	Invitrogen	NNP
cord-293966-5c466xvz	159	10	Pure	Pure	NNP
cord-293966-5c466xvz	159	11	-	-	HYPH
cord-293966-5c466xvz	159	12	Link	link	NN
cord-293966-5c466xvz	159	13	PCR	PCR	NNP
cord-293966-5c466xvz	159	14	Purification	Purification	NNP
cord-293966-5c466xvz	159	15	Kit	Kit	NNP
cord-293966-5c466xvz	159	16	because	because	IN
cord-293966-5c466xvz	159	17	it	-PRON-	PRP
cord-293966-5c466xvz	159	18	includes	include	VBZ
cord-293966-5c466xvz	159	19	a	a	DT
cord-293966-5c466xvz	159	20	buffer	buffer	NN
cord-293966-5c466xvz	159	21	that	that	WDT
cord-293966-5c466xvz	159	22	allows	allow	VBZ
cord-293966-5c466xvz	159	23	DNA	dna	NN
cord-293966-5c466xvz	159	24	products	product	NNS
cord-293966-5c466xvz	159	25	of	of	IN
cord-293966-5c466xvz	159	26	<	<	NNP
cord-293966-5c466xvz	159	27	300	300	CD
cord-293966-5c466xvz	159	28	bp	bp	NNP
cord-293966-5c466xvz	159	29	to	to	TO
cord-293966-5c466xvz	159	30	go	go	VB
cord-293966-5c466xvz	159	31	through	through	IN
cord-293966-5c466xvz	159	32	the	the	DT
cord-293966-5c466xvz	159	33	column	column	NN
cord-293966-5c466xvz	159	34	.	.	.
cord-293966-5c466xvz	160	1	This	this	DT
cord-293966-5c466xvz	160	2	helps	help	VBZ
cord-293966-5c466xvz	160	3	remove	remove	VB
cord-293966-5c466xvz	160	4	primer	primer	NN
cord-293966-5c466xvz	160	5	dimers	dimer	NNS
cord-293966-5c466xvz	160	6	from	from	IN
cord-293966-5c466xvz	160	7	the	the	DT
cord-293966-5c466xvz	160	8	PCR	PCR	NNP
cord-293966-5c466xvz	160	9	reaction	reaction	NN
cord-293966-5c466xvz	160	10	that	that	WDT
cord-293966-5c466xvz	160	11	could	could	MD
cord-293966-5c466xvz	160	12	interfere	interfere	VB
cord-293966-5c466xvz	160	13	with	with	IN
cord-293966-5c466xvz	160	14	recombination	recombination	NN
cord-293966-5c466xvz	160	15	.	.	.
cord-293966-5c466xvz	161	1	6	6	LS
cord-293966-5c466xvz	161	2	.	.	.
cord-293966-5c466xvz	162	1	A	a	DT
cord-293966-5c466xvz	162	2	42	42	CD
cord-293966-5c466xvz	162	3	C	c	NN
cord-293966-5c466xvz	162	4	shaking	shake	VBG
cord-293966-5c466xvz	162	5	water	water	NN
cord-293966-5c466xvz	162	6	bath	bath	NN
cord-293966-5c466xvz	162	7	is	be	VBZ
cord-293966-5c466xvz	162	8	essential	essential	JJ
cord-293966-5c466xvz	162	9	for	for	IN
cord-293966-5c466xvz	162	10	this	this	DT
cord-293966-5c466xvz	162	11	procedure	procedure	NN
cord-293966-5c466xvz	162	12	.	.	.
cord-293966-5c466xvz	163	1	The	the	DT
cord-293966-5c466xvz	163	2	bacterial	bacterial	JJ
cord-293966-5c466xvz	163	3	cultures	culture	NNS
cord-293966-5c466xvz	163	4	need	need	VBP
cord-293966-5c466xvz	163	5	to	to	TO
cord-293966-5c466xvz	163	6	heat	heat	VB
cord-293966-5c466xvz	163	7	up	up	RP
cord-293966-5c466xvz	163	8	to	to	TO
cord-293966-5c466xvz	163	9	42	42	CD
cord-293966-5c466xvz	163	10	C	c	NN
cord-293966-5c466xvz	163	11	quickly	quickly	RB
cord-293966-5c466xvz	163	12	to	to	TO
cord-293966-5c466xvz	163	13	properly	properly	RB
cord-293966-5c466xvz	163	14	induce	induce	VB
cord-293966-5c466xvz	163	15	the	the	DT
cord-293966-5c466xvz	163	16	Red	red	JJ
cord-293966-5c466xvz	163	17	enzymes	enzyme	NNS
cord-293966-5c466xvz	163	18	.	.	.
cord-293966-5c466xvz	164	1	A	a	DT
cord-293966-5c466xvz	164	2	shaking	shake	VBG
cord-293966-5c466xvz	164	3	water	water	NN
cord-293966-5c466xvz	164	4	bath	bath	NN
cord-293966-5c466xvz	164	5	is	be	VBZ
cord-293966-5c466xvz	164	6	significantly	significantly	RB
cord-293966-5c466xvz	164	7	better	well	JJR
cord-293966-5c466xvz	164	8	than	than	IN
cord-293966-5c466xvz	164	9	a	a	DT
cord-293966-5c466xvz	164	10	regular	regular	JJ
cord-293966-5c466xvz	164	11	shaking	shake	VBG
cord-293966-5c466xvz	164	12	incubator	incubator	NN
cord-293966-5c466xvz	164	13	at	at	IN
cord-293966-5c466xvz	164	14	quickly	quickly	RB
cord-293966-5c466xvz	164	15	transferring	transfer	VBG
cord-293966-5c466xvz	164	16	heat	heat	NN
cord-293966-5c466xvz	164	17	to	to	IN
cord-293966-5c466xvz	164	18	the	the	DT
cord-293966-5c466xvz	164	19	bacterial	bacterial	JJ
cord-293966-5c466xvz	164	20	culture	culture	NN
cord-293966-5c466xvz	164	21	.	.	.
cord-293966-5c466xvz	165	1	7	7	CD
cord-293966-5c466xvz	165	2	.	.	.
cord-293966-5c466xvz	166	1	We	-PRON-	PRP
cord-293966-5c466xvz	166	2	have	have	VBP
cord-293966-5c466xvz	166	3	tried	try	VBN
cord-293966-5c466xvz	166	4	several	several	JJ
cord-293966-5c466xvz	166	5	different	different	JJ
cord-293966-5c466xvz	166	6	transfection	transfection	NN
cord-293966-5c466xvz	166	7	reagents	reagent	NNS
cord-293966-5c466xvz	166	8	,	,	,
cord-293966-5c466xvz	166	9	and	and	CC
cord-293966-5c466xvz	166	10	Lipofectamine	Lipofectamine	NNP
cord-293966-5c466xvz	166	11	2000	2000	CD
cord-293966-5c466xvz	166	12	has	have	VBZ
cord-293966-5c466xvz	166	13	worked	work	VBN
cord-293966-5c466xvz	166	14	the	the	DT
cord-293966-5c466xvz	166	15	best	good	JJS
cord-293966-5c466xvz	166	16	for	for	IN
cord-293966-5c466xvz	166	17	us	-PRON-	PRP
cord-293966-5c466xvz	166	18	.	.	.
cord-293966-5c466xvz	167	1	That	that	DT
cord-293966-5c466xvz	167	2	does	do	VBZ
cord-293966-5c466xvz	167	3	not	not	RB
cord-293966-5c466xvz	167	4	mean	mean	VB
cord-293966-5c466xvz	167	5	other	other	JJ
cord-293966-5c466xvz	167	6	transfection	transfection	NN
cord-293966-5c466xvz	167	7	reagents	reagent	NNS
cord-293966-5c466xvz	167	8	wo	will	MD
cord-293966-5c466xvz	167	9	n't	not	RB
cord-293966-5c466xvz	167	10	work	work	VB
cord-293966-5c466xvz	167	11	,	,	,
cord-293966-5c466xvz	167	12	feel	feel	VB
cord-293966-5c466xvz	167	13	free	free	JJ
cord-293966-5c466xvz	167	14	to	to	TO
cord-293966-5c466xvz	167	15	try	try	VB
cord-293966-5c466xvz	167	16	whichever	whichever	WDT
cord-293966-5c466xvz	167	17	reagent	reagent	NN
cord-293966-5c466xvz	167	18	you	-PRON-	PRP
cord-293966-5c466xvz	167	19	prefer	prefer	VBP
cord-293966-5c466xvz	167	20	.	.	.
cord-293966-5c466xvz	168	1	8	8	CD
cord-293966-5c466xvz	168	2	.	.	.
cord-293966-5c466xvz	169	1	It	-PRON-	PRP
cord-293966-5c466xvz	169	2	is	be	VBZ
cord-293966-5c466xvz	169	3	important	important	JJ
cord-293966-5c466xvz	169	4	to	to	TO
cord-293966-5c466xvz	169	5	always	always	RB
cord-293966-5c466xvz	169	6	culture	culture	NN
cord-293966-5c466xvz	169	7	GS1783	GS1783	NNP
cord-293966-5c466xvz	169	8	cells	cell	NNS
cord-293966-5c466xvz	169	9	at	at	IN
cord-293966-5c466xvz	169	10	32	32	CD
cord-293966-5c466xvz	169	11	C	c	NN
cord-293966-5c466xvz	169	12	or	or	CC
cord-293966-5c466xvz	169	13	lower	low	JJR
cord-293966-5c466xvz	169	14	due	due	IN
cord-293966-5c466xvz	169	15	to	to	IN
cord-293966-5c466xvz	169	16	the	the	DT
cord-293966-5c466xvz	169	17	potential	potential	NN
cord-293966-5c466xvz	169	18	for	for	IN
cord-293966-5c466xvz	169	19	leaky	leaky	JJ
cord-293966-5c466xvz	169	20	expression	expression	NN
cord-293966-5c466xvz	169	21	of	of	IN
cord-293966-5c466xvz	169	22	the	the	DT
cord-293966-5c466xvz	169	23	Red	red	JJ
cord-293966-5c466xvz	169	24	enzymes	enzyme	NNS
cord-293966-5c466xvz	169	25	at	at	IN
cord-293966-5c466xvz	169	26	temperatures	temperature	NNS
cord-293966-5c466xvz	169	27	below	below	IN
cord-293966-5c466xvz	169	28	the	the	DT
cord-293966-5c466xvz	169	29	induction	induction	NN
cord-293966-5c466xvz	169	30	temperature	temperature	NN
cord-293966-5c466xvz	169	31	of	of	IN
cord-293966-5c466xvz	169	32	42	42	CD
cord-293966-5c466xvz	169	33	C.	C.	NNP
cord-293966-5c466xvz	169	34	9	9	CD
cord-293966-5c466xvz	169	35	.	.	.
cord-293966-5c466xvz	170	1	Some	some	DT
cord-293966-5c466xvz	170	2	shaking	shake	VBG
cord-293966-5c466xvz	170	3	water	water	NN
cord-293966-5c466xvz	170	4	baths	bath	NNS
cord-293966-5c466xvz	170	5	can	can	MD
cord-293966-5c466xvz	170	6	not	not	RB
cord-293966-5c466xvz	170	7	shake	shake	VB
cord-293966-5c466xvz	170	8	at	at	IN
cord-293966-5c466xvz	170	9	200	200	CD
cord-293966-5c466xvz	170	10	rpm	rpm	NN
cord-293966-5c466xvz	170	11	;	;	:
cord-293966-5c466xvz	170	12	if	if	IN
cord-293966-5c466xvz	170	13	this	this	DT
cord-293966-5c466xvz	170	14	is	be	VBZ
cord-293966-5c466xvz	170	15	the	the	DT
cord-293966-5c466xvz	170	16	case	case	NN
cord-293966-5c466xvz	170	17	,	,	,
cord-293966-5c466xvz	170	18	simply	simply	RB
cord-293966-5c466xvz	170	19	shake	shake	VB
cord-293966-5c466xvz	170	20	at	at	IN
cord-293966-5c466xvz	170	21	a	a	DT
cord-293966-5c466xvz	170	22	reasonable	reasonable	JJ
cord-293966-5c466xvz	170	23	speed	speed	NN
cord-293966-5c466xvz	170	24	for	for	IN
cord-293966-5c466xvz	170	25	the	the	DT
cord-293966-5c466xvz	170	26	shaker	shaker	NN
cord-293966-5c466xvz	170	27	.	.	.
cord-293966-5c466xvz	171	1	10	10	CD
cord-293966-5c466xvz	171	2	.	.	.
cord-293966-5c466xvz	172	1	We	-PRON-	PRP
cord-293966-5c466xvz	172	2	have	have	VBP
cord-293966-5c466xvz	172	3	occasionally	occasionally	RB
cord-293966-5c466xvz	172	4	had	have	VBN
cord-293966-5c466xvz	172	5	problems	problem	NNS
cord-293966-5c466xvz	172	6	obtaining	obtain	VBG
cord-293966-5c466xvz	172	7	a	a	DT
cord-293966-5c466xvz	172	8	PCR	PCR	NNP
cord-293966-5c466xvz	172	9	product	product	NN
cord-293966-5c466xvz	172	10	when	when	WRB
cord-293966-5c466xvz	172	11	using	use	VBG
cord-293966-5c466xvz	172	12	the	the	DT
cord-293966-5c466xvz	172	13	manufacturer	manufacturer	NN
cord-293966-5c466xvz	172	14	recommended	recommend	VBD
cord-293966-5c466xvz	172	15	0.5	0.5	CD
cord-293966-5c466xvz	172	16	μL	μl	NN
cord-293966-5c466xvz	172	17	of	of	IN
cord-293966-5c466xvz	172	18	highfidelity	highfidelity	NN
cord-293966-5c466xvz	172	19	polymerase	polymerase	NN
cord-293966-5c466xvz	172	20	.	.	.
cord-293966-5c466xvz	173	1	Using	use	VBG
cord-293966-5c466xvz	173	2	1	1	CD
cord-293966-5c466xvz	173	3	μL	μl	NN
cord-293966-5c466xvz	173	4	of	of	IN
cord-293966-5c466xvz	173	5	polymerase	polymerase	NN
cord-293966-5c466xvz	173	6	provides	provide	VBZ
cord-293966-5c466xvz	173	7	more	more	RBR
cord-293966-5c466xvz	173	8	consistent	consistent	JJ
cord-293966-5c466xvz	173	9	results	result	NNS
cord-293966-5c466xvz	173	10	.	.	.
cord-293966-5c466xvz	174	1	11	11	CD
cord-293966-5c466xvz	174	2	.	.	.
cord-293966-5c466xvz	175	1	Unlike	unlike	IN
cord-293966-5c466xvz	175	2	a	a	DT
cord-293966-5c466xvz	175	3	typical	typical	JJ
cord-293966-5c466xvz	175	4	1	1	CD
cord-293966-5c466xvz	175	5	h	h	NN
cord-293966-5c466xvz	175	6	recovery	recovery	NN
cord-293966-5c466xvz	175	7	following	follow	VBG
cord-293966-5c466xvz	175	8	electroporation	electroporation	NN
cord-293966-5c466xvz	175	9	,	,	,
cord-293966-5c466xvz	175	10	it	-PRON-	PRP
cord-293966-5c466xvz	175	11	is	be	VBZ
cord-293966-5c466xvz	175	12	important	important	JJ
cord-293966-5c466xvz	175	13	to	to	TO
cord-293966-5c466xvz	175	14	incubate	incubate	VB
cord-293966-5c466xvz	175	15	these	these	DT
cord-293966-5c466xvz	175	16	cultures	culture	NNS
cord-293966-5c466xvz	175	17	for	for	IN
cord-293966-5c466xvz	175	18	several	several	JJ
cord-293966-5c466xvz	175	19	hours	hour	NNS
cord-293966-5c466xvz	175	20	in	in	IN
cord-293966-5c466xvz	175	21	SOC	SOC	NNP
cord-293966-5c466xvz	175	22	to	to	TO
cord-293966-5c466xvz	175	23	allow	allow	VB
cord-293966-5c466xvz	175	24	time	time	NN
cord-293966-5c466xvz	175	25	for	for	IN
cord-293966-5c466xvz	175	26	recombination	recombination	NN
cord-293966-5c466xvz	175	27	to	to	TO
cord-293966-5c466xvz	175	28	occur	occur	VB
cord-293966-5c466xvz	175	29	.	.	.
cord-293966-5c466xvz	176	1	A	a	DT
cord-293966-5c466xvz	176	2	recovery	recovery	NN
cord-293966-5c466xvz	176	3	time	time	NN
cord-293966-5c466xvz	176	4	of	of	IN
cord-293966-5c466xvz	176	5	5	5	CD
cord-293966-5c466xvz	176	6	h	h	NN
cord-293966-5c466xvz	176	7	or	or	CC
cord-293966-5c466xvz	176	8	greater	great	JJR
cord-293966-5c466xvz	176	9	is	be	VBZ
cord-293966-5c466xvz	176	10	preferred	prefer	VBN
cord-293966-5c466xvz	176	11	,	,	,
cord-293966-5c466xvz	176	12	with	with	IN
cord-293966-5c466xvz	176	13	a	a	DT
cord-293966-5c466xvz	176	14	minimal	minimal	JJ
cord-293966-5c466xvz	176	15	recovery	recovery	NN
cord-293966-5c466xvz	176	16	time	time	NN
cord-293966-5c466xvz	176	17	being	be	VBG
cord-293966-5c466xvz	176	18	3	3	CD
cord-293966-5c466xvz	176	19	h.	h.	NN
cord-293966-5c466xvz	176	20	12	12	CD
cord-293966-5c466xvz	176	21	.	.	.
cord-293966-5c466xvz	177	1	Occasionally	occasionally	RB
cord-293966-5c466xvz	177	2	,	,	,
cord-293966-5c466xvz	177	3	we	-PRON-	PRP
cord-293966-5c466xvz	177	4	find	find	VBP
cord-293966-5c466xvz	177	5	that	that	IN
cord-293966-5c466xvz	177	6	following	follow	VBG
cord-293966-5c466xvz	177	7	PCR	PCR	NNP
cord-293966-5c466xvz	177	8	some	some	DT
cord-293966-5c466xvz	177	9	clones	clone	NNS
cord-293966-5c466xvz	177	10	have	have	VBP
cord-293966-5c466xvz	177	11	bands	band	NNS
cord-293966-5c466xvz	177	12	that	that	WDT
cord-293966-5c466xvz	177	13	correspond	correspond	VBP
cord-293966-5c466xvz	177	14	to	to	IN
cord-293966-5c466xvz	177	15	both	both	CC
cord-293966-5c466xvz	177	16	the	the	DT
cord-293966-5c466xvz	177	17	WT	WT	NNP
cord-293966-5c466xvz	177	18	BAC	BAC	NNP
cord-293966-5c466xvz	177	19	and	and	CC
cord-293966-5c466xvz	177	20	the	the	DT
cord-293966-5c466xvz	177	21	desired	desire	VBN
cord-293966-5c466xvz	177	22	Kan	Kan	NNP
cord-293966-5c466xvz	177	23	R	R	NNP
cord-293966-5c466xvz	177	24	-I	-I	NNP
cord-293966-5c466xvz	177	25	-	-	:
cord-293966-5c466xvz	177	26	SceI	SceI	NNP
cord-293966-5c466xvz	177	27	insert	insert	VB
cord-293966-5c466xvz	177	28	BAC	BAC	NNP
cord-293966-5c466xvz	177	29	.	.	.
cord-293966-5c466xvz	178	1	It	-PRON-	PRP
cord-293966-5c466xvz	178	2	is	be	VBZ
cord-293966-5c466xvz	178	3	likely	likely	JJ
cord-293966-5c466xvz	178	4	due	due	IN
cord-293966-5c466xvz	178	5	to	to	IN
cord-293966-5c466xvz	178	6	at	at	RB
cord-293966-5c466xvz	178	7	least	least	RBS
cord-293966-5c466xvz	178	8	two	two	CD
cord-293966-5c466xvz	178	9	copies	copy	NNS
cord-293966-5c466xvz	178	10	of	of	IN
cord-293966-5c466xvz	178	11	BAC	BAC	NNP
cord-293966-5c466xvz	178	12	DNA	DNA	NNP
cord-293966-5c466xvz	178	13	being	be	VBG
cord-293966-5c466xvz	178	14	present	present	JJ
cord-293966-5c466xvz	178	15	in	in	IN
cord-293966-5c466xvz	178	16	the	the	DT
cord-293966-5c466xvz	178	17	same	same	JJ
cord-293966-5c466xvz	178	18	cell	cell	NN
cord-293966-5c466xvz	178	19	.	.	.
cord-293966-5c466xvz	179	1	Unless	unless	IN
cord-293966-5c466xvz	179	2	the	the	DT
cord-293966-5c466xvz	179	3	molar	molar	JJ
cord-293966-5c466xvz	179	4	ratio	ratio	NN
cord-293966-5c466xvz	179	5	of	of	IN
cord-293966-5c466xvz	179	6	the	the	DT
cord-293966-5c466xvz	179	7	Kan	Kan	NNP
cord-293966-5c466xvz	179	8	R	R	NNP
cord-293966-5c466xvz	179	9	-I	-I	NNP
cord-293966-5c466xvz	179	10	-	-	:
cord-293966-5c466xvz	179	11	SceI	SceI	NNP
cord-293966-5c466xvz	179	12	insert	insert	NN
cord-293966-5c466xvz	179	13	to	to	IN
cord-293966-5c466xvz	179	14	the	the	DT
cord-293966-5c466xvz	179	15	WT	WT	NNP
cord-293966-5c466xvz	179	16	BAC	BAC	NNP
cord-293966-5c466xvz	179	17	is	be	VBZ
cord-293966-5c466xvz	179	18	very	very	RB
cord-293966-5c466xvz	179	19	large	large	JJ
cord-293966-5c466xvz	179	20	,	,	,
cord-293966-5c466xvz	179	21	these	these	DT
cord-293966-5c466xvz	179	22	clones	clone	NNS
cord-293966-5c466xvz	179	23	should	should	MD
cord-293966-5c466xvz	179	24	be	be	VB
cord-293966-5c466xvz	179	25	avoided	avoid	VBN
cord-293966-5c466xvz	179	26	.	.	.
cord-293966-5c466xvz	180	1	13	13	CD
cord-293966-5c466xvz	180	2	.	.	.
cord-293966-5c466xvz	181	1	After	after	IN
cord-293966-5c466xvz	181	2	positive	positive	JJ
cord-293966-5c466xvz	181	3	selection	selection	NN
cord-293966-5c466xvz	181	4	,	,	,
cord-293966-5c466xvz	181	5	we	-PRON-	PRP
cord-293966-5c466xvz	181	6	do	do	VBP
cord-293966-5c466xvz	181	7	not	not	RB
cord-293966-5c466xvz	181	8	check	check	VB
cord-293966-5c466xvz	181	9	the	the	DT
cord-293966-5c466xvz	181	10	BAC	BAC	NNP
cord-293966-5c466xvz	181	11	by	by	IN
cord-293966-5c466xvz	181	12	restriction	restriction	NN
cord-293966-5c466xvz	181	13	digest	digest	NN
cord-293966-5c466xvz	181	14	,	,	,
cord-293966-5c466xvz	181	15	because	because	IN
cord-293966-5c466xvz	181	16	(	(	-LRB-
cord-293966-5c466xvz	181	17	a	a	LS
cord-293966-5c466xvz	181	18	)	)	-RRB-
cord-293966-5c466xvz	181	19	we	-PRON-	PRP
cord-293966-5c466xvz	181	20	find	find	VBP
cord-293966-5c466xvz	181	21	that	that	IN
cord-293966-5c466xvz	181	22	in	in	IN
cord-293966-5c466xvz	181	23	many	many	JJ
cord-293966-5c466xvz	181	24	cases	case	NNS
cord-293966-5c466xvz	181	25	the	the	DT
cord-293966-5c466xvz	181	26	amount	amount	NN
cord-293966-5c466xvz	181	27	of	of	IN
cord-293966-5c466xvz	181	28	BAC	BAC	NNP
cord-293966-5c466xvz	181	29	DNA	DNA	NNP
cord-293966-5c466xvz	181	30	from	from	IN
cord-293966-5c466xvz	181	31	a	a	DT
cord-293966-5c466xvz	181	32	miniprep	miniprep	NN
cord-293966-5c466xvz	181	33	is	be	VBZ
cord-293966-5c466xvz	181	34	insufficient	insufficient	JJ
cord-293966-5c466xvz	181	35	for	for	IN
cord-293966-5c466xvz	181	36	a	a	DT
cord-293966-5c466xvz	181	37	readable	readable	JJ
cord-293966-5c466xvz	181	38	digest	digest	NN
cord-293966-5c466xvz	181	39	,	,	,
cord-293966-5c466xvz	181	40	and	and	CC
cord-293966-5c466xvz	181	41	(	(	-LRB-
cord-293966-5c466xvz	181	42	b	b	LS
cord-293966-5c466xvz	181	43	)	)	-RRB-
cord-293966-5c466xvz	181	44	we	-PRON-	PRP
cord-293966-5c466xvz	181	45	find	find	VBP
cord-293966-5c466xvz	181	46	that	that	IN
cord-293966-5c466xvz	181	47	the	the	DT
cord-293966-5c466xvz	181	48	colonies	colony	NNS
cord-293966-5c466xvz	181	49	that	that	WDT
cord-293966-5c466xvz	181	50	pass	pass	VBP
cord-293966-5c466xvz	181	51	the	the	DT
cord-293966-5c466xvz	181	52	replica	replica	NN
cord-293966-5c466xvz	181	53	plating	plating	NN
cord-293966-5c466xvz	181	54	and	and	CC
cord-293966-5c466xvz	181	55	PCR	PCR	NNP
cord-293966-5c466xvz	181	56	tests	test	NNS
cord-293966-5c466xvz	181	57	rarely	rarely	RB
cord-293966-5c466xvz	181	58	if	if	IN
cord-293966-5c466xvz	181	59	ever	ever	RB
cord-293966-5c466xvz	181	60	have	have	VBP
cord-293966-5c466xvz	181	61	any	any	DT
cord-293966-5c466xvz	181	62	significant	significant	JJ
cord-293966-5c466xvz	181	63	problems	problem	NNS
cord-293966-5c466xvz	181	64	concerning	concern	VBG
cord-293966-5c466xvz	181	65	removing	remove	VBG
cord-293966-5c466xvz	181	66	or	or	CC
cord-293966-5c466xvz	181	67	duplicating	duplicate	VBG
cord-293966-5c466xvz	181	68	regions	region	NNS
cord-293966-5c466xvz	181	69	of	of	IN
cord-293966-5c466xvz	181	70	the	the	DT
cord-293966-5c466xvz	181	71	MERS	MERS	NNP
cord-293966-5c466xvz	181	72	-	-	HYPH
cord-293966-5c466xvz	181	73	CoV	CoV	NNP
cord-293966-5c466xvz	181	74	BAC	BAC	NNP
cord-293966-5c466xvz	181	75	DNA	DNA	NNP
cord-293966-5c466xvz	181	76	.	.	.
cord-293966-5c466xvz	182	1	14	14	CD
cord-293966-5c466xvz	182	2	.	.	.
cord-293966-5c466xvz	183	1	First	first	RB
cord-293966-5c466xvz	183	2	,	,	,
cord-293966-5c466xvz	183	3	colonies	colony	NNS
cord-293966-5c466xvz	183	4	may	may	MD
cord-293966-5c466xvz	183	5	take	take	VB
cord-293966-5c466xvz	183	6	over	over	RP
cord-293966-5c466xvz	183	7	1	1	CD
cord-293966-5c466xvz	183	8	day	day	NN
cord-293966-5c466xvz	183	9	before	before	IN
cord-293966-5c466xvz	183	10	they	-PRON-	PRP
cord-293966-5c466xvz	183	11	are	be	VBP
cord-293966-5c466xvz	183	12	visible	visible	JJ
cord-293966-5c466xvz	183	13	.	.	.
cord-293966-5c466xvz	184	1	Second	second	RB
cord-293966-5c466xvz	184	2	,	,	,
cord-293966-5c466xvz	184	3	while	while	IN
cord-293966-5c466xvz	184	4	the	the	DT
cord-293966-5c466xvz	184	5	efficiency	efficiency	NN
cord-293966-5c466xvz	184	6	of	of	IN
cord-293966-5c466xvz	184	7	the	the	DT
cord-293966-5c466xvz	184	8	negative	negative	JJ
cord-293966-5c466xvz	184	9	selection	selection	NN
cord-293966-5c466xvz	184	10	can	can	MD
cord-293966-5c466xvz	184	11	be	be	VB
cord-293966-5c466xvz	184	12	very	very	RB
cord-293966-5c466xvz	184	13	low	low	JJ
cord-293966-5c466xvz	184	14	,	,	,
cord-293966-5c466xvz	184	15	any	any	DT
cord-293966-5c466xvz	184	16	colonies	colony	NNS
cord-293966-5c466xvz	184	17	that	that	WDT
cord-293966-5c466xvz	184	18	have	have	VBP
cord-293966-5c466xvz	184	19	grown	grow	VBN
cord-293966-5c466xvz	184	20	on	on	IN
cord-293966-5c466xvz	184	21	LB	LB	NNP
cord-293966-5c466xvz	184	22	-	-	HYPH
cord-293966-5c466xvz	184	23	cml	cml	NNP
cord-293966-5c466xvz	184	24	plates	plate	NNS
cord-293966-5c466xvz	184	25	but	but	CC
cord-293966-5c466xvz	184	26	do	do	VBP
cord-293966-5c466xvz	184	27	not	not	RB
cord-293966-5c466xvz	184	28	grow	grow	VB
cord-293966-5c466xvz	184	29	on	on	IN
cord-293966-5c466xvz	184	30	LB	LB	NNP
cord-293966-5c466xvz	184	31	-	-	HYPH
cord-293966-5c466xvz	184	32	cml	cml	NNP
cord-293966-5c466xvz	184	33	/	/	SYM
cord-293966-5c466xvz	184	34	kan	kan	NNP
cord-293966-5c466xvz	184	35	plates	plate	NNS
cord-293966-5c466xvz	184	36	are	be	VBP
cord-293966-5c466xvz	184	37	very	very	RB
cord-293966-5c466xvz	184	38	likely	likely	RB
cord-293966-5c466xvz	184	39	correct	correct	JJ
cord-293966-5c466xvz	184	40	.	.	.
cord-293966-5c466xvz	185	1	Therefore	therefore	RB
cord-293966-5c466xvz	185	2	,	,	,
cord-293966-5c466xvz	185	3	we	-PRON-	PRP
cord-293966-5c466xvz	185	4	go	go	VBP
cord-293966-5c466xvz	185	5	directly	directly	RB
cord-293966-5c466xvz	185	6	to	to	IN
cord-293966-5c466xvz	185	7	starting	start	VBG
cord-293966-5c466xvz	185	8	large	large	JJ
cord-293966-5c466xvz	185	9	-	-	HYPH
cord-293966-5c466xvz	185	10	scale	scale	NN
cord-293966-5c466xvz	185	11	cultures	culture	NNS
cord-293966-5c466xvz	185	12	for	for	IN
cord-293966-5c466xvz	185	13	these	these	DT
cord-293966-5c466xvz	185	14	clones	clone	NNS
cord-293966-5c466xvz	185	15	.	.	.
cord-293966-5c466xvz	186	1	That	that	DT
cord-293966-5c466xvz	186	2	way	way	NN
cord-293966-5c466xvz	186	3	we	-PRON-	PRP
cord-293966-5c466xvz	186	4	can	can	MD
cord-293966-5c466xvz	186	5	do	do	VB
cord-293966-5c466xvz	186	6	the	the	DT
cord-293966-5c466xvz	186	7	final	final	JJ
cord-293966-5c466xvz	186	8	diagnostic	diagnostic	JJ
cord-293966-5c466xvz	186	9	tests	test	NNS
cord-293966-5c466xvz	186	10	and	and	CC
cord-293966-5c466xvz	186	11	we	-PRON-	PRP
cord-293966-5c466xvz	186	12	can	can	MD
cord-293966-5c466xvz	186	13	prepare	prepare	VB
cord-293966-5c466xvz	186	14	for	for	IN
cord-293966-5c466xvz	186	15	the	the	DT
cord-293966-5c466xvz	186	16	BAC	BAC	NNP
cord-293966-5c466xvz	186	17	transfection	transfection	NN
cord-293966-5c466xvz	186	18	and	and	CC
cord-293966-5c466xvz	186	19	viral	viral	JJ
cord-293966-5c466xvz	186	20	recovery	recovery	NN
cord-293966-5c466xvz	186	21	at	at	IN
cord-293966-5c466xvz	186	22	the	the	DT
cord-293966-5c466xvz	186	23	same	same	JJ
cord-293966-5c466xvz	186	24	time	time	NN
cord-293966-5c466xvz	186	25	.	.	.
cord-293966-5c466xvz	187	1	15	15	CD
cord-293966-5c466xvz	187	2	.	.	.
cord-293966-5c466xvz	188	1	A	a	DT
cord-293966-5c466xvz	188	2	KpnI	KpnI	NNP
cord-293966-5c466xvz	188	3	digest	digest	NN
cord-293966-5c466xvz	188	4	of	of	IN
cord-293966-5c466xvz	188	5	pBAC	pbac	JJ
cord-293966-5c466xvz	188	6	-	-	HYPH
cord-293966-5c466xvz	188	7	MERS	MERS	NNP
cord-293966-5c466xvz	188	8	-	-	HYPH
cord-293966-5c466xvz	188	9	CoV	cov	NN
cord-293966-5c466xvz	188	10	results	result	NNS
cord-293966-5c466xvz	188	11	in	in	IN
cord-293966-5c466xvz	188	12	DNA	dna	NN
cord-293966-5c466xvz	188	13	fragments	fragment	NNS
cord-293966-5c466xvz	188	14	of	of	IN
cord-293966-5c466xvz	188	15	19.1	19.1	CD
cord-293966-5c466xvz	188	16	,	,	,
cord-293966-5c466xvz	188	17	13.8	13.8	CD
cord-293966-5c466xvz	188	18	,	,	,
cord-293966-5c466xvz	188	19	3.9	3.9	CD
cord-293966-5c466xvz	188	20	,	,	,
cord-293966-5c466xvz	188	21	and	and	CC
cord-293966-5c466xvz	188	22	1.5	1.5	CD
cord-293966-5c466xvz	188	23	kb	kb	NNS
cord-293966-5c466xvz	188	24	.	.	.
cord-293966-5c466xvz	189	1	We	-PRON-	PRP
cord-293966-5c466xvz	189	2	have	have	VBP
cord-293966-5c466xvz	189	3	found	find	VBN
cord-293966-5c466xvz	189	4	this	this	DT
cord-293966-5c466xvz	189	5	is	be	VBZ
cord-293966-5c466xvz	189	6	the	the	DT
cord-293966-5c466xvz	189	7	best	good	JJS
cord-293966-5c466xvz	189	8	digest	digest	NN
cord-293966-5c466xvz	189	9	for	for	IN
cord-293966-5c466xvz	189	10	diagnostic	diagnostic	JJ
cord-293966-5c466xvz	189	11	evaluation	evaluation	NN
cord-293966-5c466xvz	189	12	of	of	IN
cord-293966-5c466xvz	189	13	the	the	DT
cord-293966-5c466xvz	189	14	MERS	MERS	NNP
cord-293966-5c466xvz	189	15	-	-	HYPH
cord-293966-5c466xvz	189	16	CoV	cov	NN
cord-293966-5c466xvz	189	17	BAC	BAC	NNP
cord-293966-5c466xvz	189	18	;	;	:
cord-293966-5c466xvz	189	19	however	however	RB
cord-293966-5c466xvz	189	20	other	other	JJ
cord-293966-5c466xvz	189	21	enzymes	enzyme	NNS
cord-293966-5c466xvz	189	22	or	or	CC
cord-293966-5c466xvz	189	23	enzyme	enzyme	NN
cord-293966-5c466xvz	189	24	combinations	combination	NNS
cord-293966-5c466xvz	189	25	will	will	MD
cord-293966-5c466xvz	189	26	work	work	VB
cord-293966-5c466xvz	189	27	as	as	RB
cord-293966-5c466xvz	189	28	well	well	RB
cord-293966-5c466xvz	189	29	.	.	.
cord-293966-5c466xvz	190	1	Be	be	VB
cord-293966-5c466xvz	190	2	sure	sure	JJ
cord-293966-5c466xvz	190	3	to	to	TO
cord-293966-5c466xvz	190	4	use	use	VB
cord-293966-5c466xvz	190	5	a	a	DT
cord-293966-5c466xvz	190	6	low	low	JJ
cord-293966-5c466xvz	190	7	-	-	HYPH
cord-293966-5c466xvz	190	8	percentage	percentage	NN
cord-293966-5c466xvz	190	9	agarose	agarose	NN
cord-293966-5c466xvz	190	10	gel	gel	NN
cord-293966-5c466xvz	190	11	to	to	TO
cord-293966-5c466xvz	190	12	effectively	effectively	RB
cord-293966-5c466xvz	190	13	separate	separate	VB
cord-293966-5c466xvz	190	14	the	the	DT
cord-293966-5c466xvz	190	15	large	large	JJ
cord-293966-5c466xvz	190	16	DNA	dna	NN
cord-293966-5c466xvz	190	17	molecules	molecule	NNS
cord-293966-5c466xvz	190	18	.	.	.
cord-293966-5c466xvz	191	1	16	16	CD
cord-293966-5c466xvz	191	2	.	.	.
cord-293966-5c466xvz	192	1	Other	other	JJ
cord-293966-5c466xvz	192	2	laboratories	laboratory	NNS
cord-293966-5c466xvz	192	3	use	use	VBP
cord-293966-5c466xvz	192	4	BHK-21	BHK-21	NNP
cord-293966-5c466xvz	192	5	cells	cell	NNS
cord-293966-5c466xvz	192	6	for	for	IN
cord-293966-5c466xvz	192	7	the	the	DT
cord-293966-5c466xvz	192	8	initial	initial	JJ
cord-293966-5c466xvz	192	9	transfection	transfection	NN
cord-293966-5c466xvz	192	10	since	since	IN
cord-293966-5c466xvz	192	11	these	these	DT
cord-293966-5c466xvz	192	12	cells	cell	NNS
cord-293966-5c466xvz	192	13	are	be	VBP
cord-293966-5c466xvz	192	14	highly	highly	RB
cord-293966-5c466xvz	192	15	transfectable	transfectable	JJ
cord-293966-5c466xvz	192	16	.	.	.
cord-293966-5c466xvz	193	1	The	the	DT
cord-293966-5c466xvz	193	2	transfected	transfecte	VBN
cord-293966-5c466xvz	193	3	cells	cell	NNS
cord-293966-5c466xvz	193	4	can	can	MD
cord-293966-5c466xvz	193	5	be	be	VB
cord-293966-5c466xvz	193	6	overlaid	overlay	VBN
cord-293966-5c466xvz	193	7	on	on	IN
cord-293966-5c466xvz	193	8	the	the	DT
cord-293966-5c466xvz	193	9	Vero-81	vero-81	NN
cord-293966-5c466xvz	193	10	or	or	CC
cord-293966-5c466xvz	193	11	Huh-7	Huh-7	NNP
cord-293966-5c466xvz	193	12	cells	cell	NNS
cord-293966-5c466xvz	193	13	for	for	IN
cord-293966-5c466xvz	193	14	further	further	JJ
cord-293966-5c466xvz	193	15	outgrowth	outgrowth	NN
cord-293966-5c466xvz	193	16	of	of	IN
cord-293966-5c466xvz	193	17	the	the	DT
cord-293966-5c466xvz	193	18	recombinant	recombinant	JJ
cord-293966-5c466xvz	193	19	virus	virus	NN
cord-293966-5c466xvz	193	20	.	.	.
cord-293966-5c466xvz	194	1	17	17	CD
cord-293966-5c466xvz	194	2	.	.	.
cord-293966-5c466xvz	195	1	It	-PRON-	PRP
cord-293966-5c466xvz	195	2	is	be	VBZ
cord-293966-5c466xvz	195	3	feasible	feasible	JJ
cord-293966-5c466xvz	195	4	to	to	TO
cord-293966-5c466xvz	195	5	wait	wait	VB
cord-293966-5c466xvz	195	6	o	o	NN
cord-293966-5c466xvz	195	7	/	/	SYM
cord-293966-5c466xvz	195	8	n	n	NN
cord-293966-5c466xvz	195	9	to	to	TO
cord-293966-5c466xvz	195	10	change	change	VB
cord-293966-5c466xvz	195	11	the	the	DT
cord-293966-5c466xvz	195	12	media	medium	NNS
cord-293966-5c466xvz	195	13	of	of	IN
cord-293966-5c466xvz	195	14	the	the	DT
cord-293966-5c466xvz	195	15	transfected	transfecte	VBN
cord-293966-5c466xvz	195	16	Vero	Vero	NNP
cord-293966-5c466xvz	195	17	81	81	CD
cord-293966-5c466xvz	195	18	cells	cell	NNS
cord-293966-5c466xvz	195	19	,	,	,
cord-293966-5c466xvz	195	20	but	but	CC
cord-293966-5c466xvz	195	21	this	this	DT
cord-293966-5c466xvz	195	22	does	do	VBZ
cord-293966-5c466xvz	195	23	result	result	VB
cord-293966-5c466xvz	195	24	in	in	IN
cord-293966-5c466xvz	195	25	an	an	DT
cord-293966-5c466xvz	195	26	increase	increase	NN
cord-293966-5c466xvz	195	27	in	in	IN
cord-293966-5c466xvz	195	28	the	the	DT
cord-293966-5c466xvz	195	29	cytotoxicity	cytotoxicity	NN
cord-293966-5c466xvz	195	30	induced	induce	VBN
cord-293966-5c466xvz	195	31	by	by	IN
cord-293966-5c466xvz	195	32	Lipofectamine	Lipofectamine	NNP
cord-293966-5c466xvz	195	33	.	.	.
cord-293966-5c466xvz	196	1	This	this	DT
cord-293966-5c466xvz	196	2	is	be	VBZ
cord-293966-5c466xvz	196	3	not	not	RB
cord-293966-5c466xvz	196	4	feasible	feasible	JJ
cord-293966-5c466xvz	196	5	if	if	IN
cord-293966-5c466xvz	196	6	you	-PRON-	PRP
cord-293966-5c466xvz	196	7	are	be	VBP
cord-293966-5c466xvz	196	8	using	use	VBG
cord-293966-5c466xvz	196	9	Huh-7	Huh-7	NNP
cord-293966-5c466xvz	196	10	cells	cell	NNS
cord-293966-5c466xvz	196	11	.	.	.
cord-293966-5c466xvz	197	1	18	18	CD
cord-293966-5c466xvz	197	2	.	.	.
cord-293966-5c466xvz	198	1	Mutant	mutant	JJ
cord-293966-5c466xvz	198	2	viruses	virus	NNS
cord-293966-5c466xvz	198	3	that	that	WDT
cord-293966-5c466xvz	198	4	do	do	VBP
cord-293966-5c466xvz	198	5	not	not	RB
cord-293966-5c466xvz	198	6	replicate	replicate	VB
cord-293966-5c466xvz	198	7	as	as	RB
cord-293966-5c466xvz	198	8	well	well	RB
cord-293966-5c466xvz	198	9	as	as	IN
cord-293966-5c466xvz	198	10	WT	WT	NNP
cord-293966-5c466xvz	198	11	virus	virus	NN
cord-293966-5c466xvz	198	12	may	may	MD
cord-293966-5c466xvz	198	13	require	require	VB
cord-293966-5c466xvz	198	14	increased	increased	JJ
cord-293966-5c466xvz	198	15	amounts	amount	NNS
cord-293966-5c466xvz	198	16	of	of	IN
cord-293966-5c466xvz	198	17	P0	p0	NN
cord-293966-5c466xvz	198	18	virus	virus	NN
cord-293966-5c466xvz	198	19	.	.	.
cord-293966-5c466xvz	199	1	19	19	CD
cord-293966-5c466xvz	199	2	.	.	.
cord-293966-5c466xvz	200	1	It	-PRON-	PRP
cord-293966-5c466xvz	200	2	is	be	VBZ
cord-293966-5c466xvz	200	3	also	also	RB
cord-293966-5c466xvz	200	4	beneficial	beneficial	JJ
cord-293966-5c466xvz	200	5	to	to	TO
cord-293966-5c466xvz	200	6	check	check	VB
cord-293966-5c466xvz	200	7	the	the	DT
cord-293966-5c466xvz	200	8	integrity	integrity	NN
cord-293966-5c466xvz	200	9	of	of	IN
cord-293966-5c466xvz	200	10	the	the	DT
cord-293966-5c466xvz	200	11	entire	entire	JJ
cord-293966-5c466xvz	200	12	MERS	MERS	NNP
cord-293966-5c466xvz	200	13	-	-	HYPH
cord-293966-5c466xvz	200	14	CoV	cov	NN
cord-293966-5c466xvz	200	15	genome	genome	NN
cord-293966-5c466xvz	200	16	by	by	IN
cord-293966-5c466xvz	200	17	RT	RT	NNP
cord-293966-5c466xvz	200	18	-	-	HYPH
cord-293966-5c466xvz	200	19	PCR	PCR	NNP
cord-293966-5c466xvz	200	20	after	after	IN
cord-293966-5c466xvz	200	21	several	several	JJ
cord-293966-5c466xvz	200	22	passages	passage	NNS
cord-293966-5c466xvz	200	23	as	as	IN
cord-293966-5c466xvz	200	24	MERS	MERS	NNP
cord-293966-5c466xvz	200	25	-	-	HYPH
cord-293966-5c466xvz	200	26	CoV	CoV	NNP
cord-293966-5c466xvz	200	27	tends	tend	VBZ
cord-293966-5c466xvz	200	28	to	to	TO
cord-293966-5c466xvz	200	29	occasionally	occasionally	RB
cord-293966-5c466xvz	200	30	delete	delete	VB
cord-293966-5c466xvz	200	31	sections	section	NNS
cord-293966-5c466xvz	200	32	of	of	IN
cord-293966-5c466xvz	200	33	the	the	DT
cord-293966-5c466xvz	200	34	accessory	accessory	NN
cord-293966-5c466xvz	200	35	proteins	protein	NNS
cord-293966-5c466xvz	200	36	.	.	.
cord-293966-5c466xvz	201	1	Middle	Middle	NNP
cord-293966-5c466xvz	201	2	East	East	NNP
cord-293966-5c466xvz	201	3	respiratory	respiratory	JJ
cord-293966-5c466xvz	201	4	syndrome	syndrome	NN
cord-293966-5c466xvz	201	5	:	:	:
cord-293966-5c466xvz	201	6	emergence	emergence	NN
cord-293966-5c466xvz	201	7	of	of	IN
cord-293966-5c466xvz	201	8	a	a	DT
cord-293966-5c466xvz	201	9	pathogenic	pathogenic	JJ
cord-293966-5c466xvz	201	10	human	human	JJ
cord-293966-5c466xvz	201	11	coronavirus	coronavirus	NN
cord-293966-5c466xvz	201	12	Engineering	engineer	VBG
cord-293966-5c466xvz	201	13	a	a	DT
cord-293966-5c466xvz	201	14	replication	replication	NN
cord-293966-5c466xvz	201	15	-	-	HYPH
cord-293966-5c466xvz	201	16	competent	competent	JJ
cord-293966-5c466xvz	201	17	,	,	,
cord-293966-5c466xvz	201	18	propagation	propagation	NN
cord-293966-5c466xvz	201	19	-	-	HYPH
cord-293966-5c466xvz	201	20	defective	defective	JJ
cord-293966-5c466xvz	201	21	Middle	Middle	NNP
cord-293966-5c466xvz	201	22	East	East	NNP
cord-293966-5c466xvz	201	23	respiratory	respiratory	JJ
cord-293966-5c466xvz	201	24	syndrome	syndrome	NN
cord-293966-5c466xvz	201	25	coronavirus	coronavirus	NN
cord-293966-5c466xvz	201	26	as	as	IN
cord-293966-5c466xvz	201	27	a	a	DT
cord-293966-5c466xvz	201	28	vaccine	vaccine	NN
cord-293966-5c466xvz	201	29	candidate	candidate	NN
cord-293966-5c466xvz	201	30	Reverse	reverse	VB
cord-293966-5c466xvz	201	31	genetics	genetic	NNS
cord-293966-5c466xvz	201	32	with	with	IN
cord-293966-5c466xvz	201	33	a	a	DT
cord-293966-5c466xvz	201	34	full	full	JJ
cord-293966-5c466xvz	201	35	-	-	HYPH
cord-293966-5c466xvz	201	36	length	length	NN
cord-293966-5c466xvz	201	37	infectious	infectious	JJ
cord-293966-5c466xvz	201	38	cDNA	cdna	NN
cord-293966-5c466xvz	201	39	of	of	IN
cord-293966-5c466xvz	201	40	the	the	DT
cord-293966-5c466xvz	201	41	Middle	Middle	NNP
cord-293966-5c466xvz	201	42	East	East	NNP
cord-293966-5c466xvz	201	43	respiratory	respiratory	JJ
cord-293966-5c466xvz	201	44	syndrome	syndrome	NN
cord-293966-5c466xvz	201	45	coronavirus	coronavirus	NN
cord-293966-5c466xvz	201	46	Engineering	Engineering	NNP
cord-293966-5c466xvz	201	47	the	the	DT
cord-293966-5c466xvz	201	48	largest	large	JJS
cord-293966-5c466xvz	201	49	RNA	RNA	NNP
cord-293966-5c466xvz	201	50	virus	virus	NN
cord-293966-5c466xvz	201	51	genome	genome	NN
cord-293966-5c466xvz	201	52	as	as	IN
cord-293966-5c466xvz	201	53	an	an	DT
cord-293966-5c466xvz	201	54	infectious	infectious	JJ
cord-293966-5c466xvz	201	55	bacterial	bacterial	JJ
cord-293966-5c466xvz	201	56	artificial	artificial	JJ
cord-293966-5c466xvz	201	57	chromosome	chromosome	NN
cord-293966-5c466xvz	201	58	Systematic	systematic	JJ
cord-293966-5c466xvz	201	59	assembly	assembly	NN
cord-293966-5c466xvz	201	60	of	of	IN
cord-293966-5c466xvz	201	61	a	a	DT
cord-293966-5c466xvz	201	62	full	full	JJ
cord-293966-5c466xvz	201	63	-	-	HYPH
cord-293966-5c466xvz	201	64	length	length	NN
cord-293966-5c466xvz	201	65	infectious	infectious	JJ
cord-293966-5c466xvz	201	66	cDNA	cdna	NN
cord-293966-5c466xvz	201	67	of	of	IN
cord-293966-5c466xvz	201	68	mouse	mouse	NNP
cord-293966-5c466xvz	201	69	hepatitis	hepatitis	NNP
cord-293966-5c466xvz	201	70	virus	virus	NN
cord-293966-5c466xvz	201	71	strain	strain	VBP
cord-293966-5c466xvz	201	72	A59	A59	NNP
cord-293966-5c466xvz	201	73	Strategy	Strategy	NNP
cord-293966-5c466xvz	201	74	for	for	IN
cord-293966-5c466xvz	201	75	systematic	systematic	JJ
cord-293966-5c466xvz	201	76	assembly	assembly	NN
cord-293966-5c466xvz	201	77	of	of	IN
cord-293966-5c466xvz	201	78	large	large	JJ
cord-293966-5c466xvz	201	79	RNA	RNA	NNP
cord-293966-5c466xvz	201	80	and	and	CC
cord-293966-5c466xvz	201	81	DNA	dna	NN
cord-293966-5c466xvz	201	82	genomes	genome	NNS
cord-293966-5c466xvz	201	83	:	:	:
cord-293966-5c466xvz	201	84	transmissible	transmissible	JJ
cord-293966-5c466xvz	201	85	gastroenteritis	gastroenteritis	JJ
cord-293966-5c466xvz	201	86	virus	virus	NN
cord-293966-5c466xvz	201	87	model	model	NN
cord-293966-5c466xvz	201	88	Construction	construction	NN
cord-293966-5c466xvz	201	89	of	of	IN
cord-293966-5c466xvz	201	90	a	a	DT
cord-293966-5c466xvz	201	91	severe	severe	JJ
cord-293966-5c466xvz	201	92	acute	acute	JJ
cord-293966-5c466xvz	201	93	respiratory	respiratory	JJ
cord-293966-5c466xvz	201	94	syndrome	syndrome	NN
cord-293966-5c466xvz	201	95	coronavirus	coronavirus	NN
cord-293966-5c466xvz	201	96	infectious	infectious	JJ
cord-293966-5c466xvz	201	97	cDNA	cdna	NN
cord-293966-5c466xvz	201	98	clone	clone	NN
cord-293966-5c466xvz	201	99	and	and	CC
cord-293966-5c466xvz	201	100	a	a	DT
cord-293966-5c466xvz	201	101	replicon	replicon	NN
cord-293966-5c466xvz	201	102	to	to	TO
cord-293966-5c466xvz	201	103	study	study	VB
cord-293966-5c466xvz	201	104	coronavirus	coronavirus	NN
cord-293966-5c466xvz	201	105	RNA	RNA	NNP
cord-293966-5c466xvz	201	106	synthesis	synthesis	NN
cord-293966-5c466xvz	201	107	Molecular	molecular	JJ
cord-293966-5c466xvz	201	108	characterization	characterization	NN
cord-293966-5c466xvz	201	109	of	of	IN
cord-293966-5c466xvz	201	110	feline	feline	JJ
cord-293966-5c466xvz	201	111	infectious	infectious	JJ
cord-293966-5c466xvz	201	112	peritonitis	peritonitis	NN
cord-293966-5c466xvz	201	113	virus	virus	NN
cord-293966-5c466xvz	201	114	strain	strain	VBP
cord-293966-5c466xvz	201	115	DF-2	DF-2	NNP
cord-293966-5c466xvz	201	116	and	and	CC
cord-293966-5c466xvz	201	117	studies	study	NNS
cord-293966-5c466xvz	201	118	of	of	IN
cord-293966-5c466xvz	201	119	the	the	DT
cord-293966-5c466xvz	201	120	role	role	NN
cord-293966-5c466xvz	201	121	of	of	IN
cord-293966-5c466xvz	201	122	ORF3abc	orf3abc	XX
cord-293966-5c466xvz	201	123	in	in	IN
cord-293966-5c466xvz	201	124	viral	viral	JJ
cord-293966-5c466xvz	201	125	cell	cell	NN
cord-293966-5c466xvz	201	126	tropism	tropism	NN
cord-293966-5c466xvz	202	1	The	the	DT
cord-293966-5c466xvz	202	2	nsp3	nsp3	NNS
cord-293966-5c466xvz	202	3	macrodomain	macrodomain	NN
cord-293966-5c466xvz	202	4	promotes	promote	VBZ
cord-293966-5c466xvz	202	5	virulence	virulence	NN
cord-293966-5c466xvz	202	6	in	in	IN
cord-293966-5c466xvz	202	7	mice	mouse	NNS
cord-293966-5c466xvz	202	8	with	with	IN
cord-293966-5c466xvz	202	9	coronavirus	coronavirus	NN
cord-293966-5c466xvz	202	10	-	-	HYPH
cord-293966-5c466xvz	202	11	induced	induce	VBN
cord-293966-5c466xvz	202	12	encephalitis	encephalitis	NN
cord-293966-5c466xvz	202	13	Recovery	recovery	NN
cord-293966-5c466xvz	202	14	of	of	IN
cord-293966-5c466xvz	202	15	a	a	DT
cord-293966-5c466xvz	202	16	neurovirulent	neurovirulent	NN
cord-293966-5c466xvz	202	17	human	human	JJ
cord-293966-5c466xvz	202	18	coronavirus	coronavirus	NN
cord-293966-5c466xvz	202	19	OC43	OC43	NNP
cord-293966-5c466xvz	202	20	from	from	IN
cord-293966-5c466xvz	202	21	an	an	DT
cord-293966-5c466xvz	202	22	infectious	infectious	JJ
cord-293966-5c466xvz	202	23	cDNA	cdna	NN
cord-293966-5c466xvz	202	24	clone	clone	NN
cord-293966-5c466xvz	202	25	Development	Development	NNP
cord-293966-5c466xvz	202	26	of	of	IN
cord-293966-5c466xvz	202	27	the	the	DT
cord-293966-5c466xvz	202	28	full	full	JJ
cord-293966-5c466xvz	202	29	-	-	HYPH
cord-293966-5c466xvz	202	30	length	length	NN
cord-293966-5c466xvz	202	31	cDNA	cdna	NN
cord-293966-5c466xvz	202	32	clones	clone	NNS
cord-293966-5c466xvz	202	33	of	of	IN
cord-293966-5c466xvz	202	34	two	two	CD
cord-293966-5c466xvz	202	35	porcine	porcine	JJ
cord-293966-5c466xvz	202	36	epidemic	epidemic	NN
cord-293966-5c466xvz	202	37	diarrhea	diarrhea	NN
cord-293966-5c466xvz	202	38	disease	disease	NN
cord-293966-5c466xvz	202	39	virus	virus	NN
cord-293966-5c466xvz	202	40	isolates	isolate	VBZ
cord-293966-5c466xvz	202	41	with	with	IN
cord-293966-5c466xvz	202	42	different	different	JJ
cord-293966-5c466xvz	202	43	virulence	virulence	NN
cord-293966-5c466xvz	202	44	Bat	Bat	NNP
cord-293966-5c466xvz	202	45	severe	severe	JJ
cord-293966-5c466xvz	202	46	acute	acute	JJ
cord-293966-5c466xvz	202	47	respiratory	respiratory	JJ
cord-293966-5c466xvz	202	48	syndrome	syndrome	NN
cord-293966-5c466xvz	202	49	-	-	HYPH
cord-293966-5c466xvz	202	50	like	like	JJ
cord-293966-5c466xvz	203	1	coronavirus	coronavirus	NN
cord-293966-5c466xvz	203	2	WIV1	WIV1	NNP
cord-293966-5c466xvz	203	3	encodes	encode	VBZ
cord-293966-5c466xvz	203	4	an	an	DT
cord-293966-5c466xvz	203	5	extra	extra	JJ
cord-293966-5c466xvz	203	6	accessory	accessory	NN
cord-293966-5c466xvz	203	7	protein	protein	NN
cord-293966-5c466xvz	203	8	,	,	,
cord-293966-5c466xvz	203	9	ORFX	ORFX	NNP
cord-293966-5c466xvz	203	10	,	,	,
cord-293966-5c466xvz	203	11	involved	involve	VBN
cord-293966-5c466xvz	203	12	in	in	IN
cord-293966-5c466xvz	203	13	modulation	modulation	NN
cord-293966-5c466xvz	203	14	of	of	IN
cord-293966-5c466xvz	203	15	the	the	DT
cord-293966-5c466xvz	203	16	host	host	NN
cord-293966-5c466xvz	203	17	immune	immune	JJ
cord-293966-5c466xvz	203	18	response	response	NN
cord-293966-5c466xvz	203	19	Use	Use	NNP
cord-293966-5c466xvz	203	20	of	of	IN
cord-293966-5c466xvz	203	21	bacteriophage	bacteriophage	NN
cord-293966-5c466xvz	203	22	lambda	lambda	NN
cord-293966-5c466xvz	203	23	recombination	recombination	NN
cord-293966-5c466xvz	203	24	functions	function	NNS
cord-293966-5c466xvz	203	25	to	to	TO
cord-293966-5c466xvz	203	26	promote	promote	VB
cord-293966-5c466xvz	203	27	gene	gene	NN
cord-293966-5c466xvz	203	28	replacement	replacement	NN
cord-293966-5c466xvz	203	29	in	in	IN
cord-293966-5c466xvz	203	30	Escherichia	Escherichia	NNP
cord-293966-5c466xvz	203	31	coli	coli	NNS
cord-293966-5c466xvz	204	1	In	in	IN
cord-293966-5c466xvz	204	2	vivo	vivo	FW
cord-293966-5c466xvz	204	3	recombineering	recombineering	NN
cord-293966-5c466xvz	204	4	of	of	IN
cord-293966-5c466xvz	204	5	bacteriophage	bacteriophage	NN
cord-293966-5c466xvz	204	6	lambda	lambda	NN
cord-293966-5c466xvz	204	7	by	by	IN
cord-293966-5c466xvz	204	8	PCR	PCR	NNP
cord-293966-5c466xvz	204	9	fragments	fragment	NNS
cord-293966-5c466xvz	204	10	and	and	CC
cord-293966-5c466xvz	204	11	single	single	JJ
cord-293966-5c466xvz	204	12	-	-	HYPH
cord-293966-5c466xvz	204	13	strand	strand	NN
cord-293966-5c466xvz	204	14	oligonucleotides	oligonucleotide	NNS
cord-293966-5c466xvz	205	1	A	a	DT
cord-293966-5c466xvz	205	2	highly	highly	RB
cord-293966-5c466xvz	205	3	efficient	efficient	JJ
cord-293966-5c466xvz	205	4	Escherichia	escherichia	NN
cord-293966-5c466xvz	205	5	coli	coli	NNS
cord-293966-5c466xvz	205	6	-	-	HYPH
cord-293966-5c466xvz	205	7	based	base	VBN
cord-293966-5c466xvz	205	8	chromosome	chromosome	NN
cord-293966-5c466xvz	205	9	engineering	engineering	NN
cord-293966-5c466xvz	205	10	system	system	NN
cord-293966-5c466xvz	205	11	adapted	adapt	VBN
cord-293966-5c466xvz	205	12	for	for	IN
cord-293966-5c466xvz	205	13	recombinogenic	recombinogenic	JJ
cord-293966-5c466xvz	205	14	targeting	targeting	NN
cord-293966-5c466xvz	205	15	and	and	CC
cord-293966-5c466xvz	205	16	subcloning	subcloning	NN
cord-293966-5c466xvz	205	17	of	of	IN
cord-293966-5c466xvz	205	18	BAC	BAC	NNP
cord-293966-5c466xvz	205	19	DNA	DNA	NNP
cord-293966-5c466xvz	206	1	A	a	DT
cord-293966-5c466xvz	206	2	new	new	JJ
cord-293966-5c466xvz	206	3	logic	logic	NN
cord-293966-5c466xvz	206	4	for	for	IN
cord-293966-5c466xvz	206	5	DNA	dna	NN
cord-293966-5c466xvz	206	6	engineering	engineering	NN
cord-293966-5c466xvz	206	7	using	use	VBG
cord-293966-5c466xvz	206	8	recombination	recombination	NN
cord-293966-5c466xvz	206	9	in	in	IN
cord-293966-5c466xvz	206	10	Escherichia	Escherichia	NNP
cord-293966-5c466xvz	206	11	coli	coli	NNS
cord-293966-5c466xvz	207	1	Simple	simple	JJ
cord-293966-5c466xvz	207	2	and	and	CC
cord-293966-5c466xvz	207	3	highly	highly	RB
cord-293966-5c466xvz	207	4	efficient	efficient	JJ
cord-293966-5c466xvz	207	5	BAC	BAC	NNP
cord-293966-5c466xvz	207	6	recombineering	recombineere	VBG
cord-293966-5c466xvz	207	7	using	use	VBG
cord-293966-5c466xvz	207	8	galK	galK	NNP
cord-293966-5c466xvz	207	9	selection	selection	NN
cord-293966-5c466xvz	207	10	Two	two	CD
cord-293966-5c466xvz	207	11	-	-	HYPH
cord-293966-5c466xvz	207	12	step	step	NN
cord-293966-5c466xvz	207	13	red	red	JJ
cord-293966-5c466xvz	207	14	-	-	HYPH
cord-293966-5c466xvz	207	15	mediated	mediate	VBN
cord-293966-5c466xvz	207	16	recombination	recombination	NN
cord-293966-5c466xvz	207	17	for	for	IN
cord-293966-5c466xvz	207	18	versatile	versatile	JJ
cord-293966-5c466xvz	207	19	high	high	JJ
cord-293966-5c466xvz	207	20	-	-	HYPH
cord-293966-5c466xvz	207	21	efficiency	efficiency	NN
cord-293966-5c466xvz	207	22	markerless	markerless	NN
cord-293966-5c466xvz	207	23	DNA	dna	NN
cord-293966-5c466xvz	207	24	manipulation	manipulation	NN
cord-293966-5c466xvz	207	25	in	in	IN
cord-293966-5c466xvz	207	26	Escherichia	Escherichia	NNP
cord-293966-5c466xvz	207	27	coli	coli	NNS
cord-293966-5c466xvz	207	28	Engineering	engineering	NN
cord-293966-5c466xvz	208	1	infectious	infectious	JJ
cord-293966-5c466xvz	208	2	cDNAs	cdna	NNS
cord-293966-5c466xvz	208	3	of	of	IN
cord-293966-5c466xvz	208	4	coronavirus	coronavirus	NN
cord-293966-5c466xvz	208	5	as	as	IN
cord-293966-5c466xvz	208	6	bacterial	bacterial	JJ
cord-293966-5c466xvz	208	7	artificial	artificial	JJ
cord-293966-5c466xvz	208	8	chromosomes	chromosome	NNS
cord-293966-5c466xvz	208	9	En	en	IN
cord-293966-5c466xvz	208	10	passant	passant	NN
cord-293966-5c466xvz	208	11	mutagenesis	mutagenesis	NN
cord-293966-5c466xvz	209	1	:	:	:
cord-293966-5c466xvz	209	2	a	a	DT
cord-293966-5c466xvz	209	3	two	two	CD
cord-293966-5c466xvz	209	4	step	step	NN
cord-293966-5c466xvz	209	5	markerless	markerless	NN
cord-293966-5c466xvz	209	6	red	red	JJ
cord-293966-5c466xvz	209	7	recombination	recombination	NN
cord-293966-5c466xvz	209	8	system	system	NN
cord-293966-5c466xvz	210	1	Acknowledgments	acknowledgment	NNS
cord-293966-5c466xvz	211	1	I	-PRON-	PRP
cord-293966-5c466xvz	211	2	thank	thank	VBP
cord-293966-5c466xvz	211	3	Jeremiah	Jeremiah	NNP
cord-293966-5c466xvz	211	4	Athmer	Athmer	NNP
cord-293966-5c466xvz	211	5	and	and	CC
cord-293966-5c466xvz	211	6	Andrea	Andrea	NNP
cord-293966-5c466xvz	211	7	Pruijssers	Pruijssers	NNP
cord-293966-5c466xvz	211	8	for	for	IN
cord-293966-5c466xvz	211	9	their	-PRON-	PRP$
cord-293966-5c466xvz	211	10	substantial	substantial	JJ
cord-293966-5c466xvz	211	11	modifications	modification	NNS
cord-293966-5c466xvz	211	12	and	and	CC
cord-293966-5c466xvz	211	13	improvements	improvement	NNS
cord-293966-5c466xvz	211	14	to	to	IN
cord-293966-5c466xvz	211	15	this	this	DT
cord-293966-5c466xvz	211	16	protocol	protocol	NN
cord-293966-5c466xvz	211	17	over	over	IN
cord-293966-5c466xvz	211	18	the	the	DT
cord-293966-5c466xvz	211	19	years	year	NNS
cord-293966-5c466xvz	211	20	.	.	.
cord-293966-5c466xvz	212	1	I	-PRON-	PRP
cord-293966-5c466xvz	212	2	also	also	RB
cord-293966-5c466xvz	212	3	thank	thank	VBP
cord-293966-5c466xvz	212	4	Catherine	Catherine	NNP
cord-293966-5c466xvz	212	5	Kerr	Kerr	NNP
cord-293966-5c466xvz	212	6	,	,	,
cord-293966-5c466xvz	212	7	Ethan	Ethan	NNP
cord-293966-5c466xvz	212	8	Doerger	Doerger	NNP
cord-293966-5c466xvz	212	9	,	,	,
cord-293966-5c466xvz	212	10	Andrea	Andrea	NNP
cord-293966-5c466xvz	212	11	Pruijssers	Pruijssers	NNP
cord-293966-5c466xvz	212	12	,	,	,
cord-293966-5c466xvz	212	13	Isabel	Isabel	NNP
cord-293966-5c466xvz	212	14	Sola	Sola	NNP
cord-293966-5c466xvz	212	15	,	,	,
cord-293966-5c466xvz	212	16	and	and	CC
cord-293966-5c466xvz	212	17	Luis	Luis	NNP
cord-293966-5c466xvz	212	18	Enjuanes	Enjuanes	NNP
cord-293966-5c466xvz	212	19	for	for	IN
cord-293966-5c466xvz	212	20	critical	critical	JJ
cord-293966-5c466xvz	212	21	reading	reading	NN
cord-293966-5c466xvz	212	22	of	of	IN
cord-293966-5c466xvz	212	23	this	this	DT
cord-293966-5c466xvz	212	24	manuscript	manuscript	NN
cord-293966-5c466xvz	212	25	.	.	.
cord-293966-5c466xvz	213	1	This	this	DT
cord-293966-5c466xvz	213	2	work	work	NN
cord-293966-5c466xvz	213	3	was	be	VBD
cord-293966-5c466xvz	213	4	supported	support	VBN
cord-293966-5c466xvz	213	5	in	in	IN
cord-293966-5c466xvz	213	6	part	part	NN
cord-293966-5c466xvz	213	7	by	by	IN
cord-293966-5c466xvz	213	8	NIH	NIH	NNP
cord-293966-5c466xvz	213	9	grants	grant	NNS
cord-293966-5c466xvz	213	10	CoBRE	cobre	JJ
cord-293966-5c466xvz	214	1	P20	P20	NNP
cord-293966-5c466xvz	214	2	GM113117	GM113117	NNP
cord-293966-5c466xvz	214	3	-	-	NN
cord-293966-5c466xvz	214	4	02	02	CD
cord-293966-5c466xvz	215	1	and	and	CC
cord-293966-5c466xvz	215	2	K22	K22	NNP
cord-293966-5c466xvz	215	3	AI134993	AI134993	NNP
cord-293966-5c466xvz	215	4	-	-	HYPH
cord-293966-5c466xvz	215	5	01	01	CD
cord-293966-5c466xvz	215	6	,	,	,
cord-293966-5c466xvz	215	7	and	and	CC
cord-293966-5c466xvz	215	8	start	start	VB
cord-293966-5c466xvz	215	9	-	-	HYPH
cord-293966-5c466xvz	215	10	up	up	RP
cord-293966-5c466xvz	215	11	funds	fund	NNS
cord-293966-5c466xvz	215	12	from	from	IN
cord-293966-5c466xvz	215	13	the	the	DT
cord-293966-5c466xvz	215	14	University	University	NNP
cord-293966-5c466xvz	215	15	of	of	IN
cord-293966-5c466xvz	215	16	Kansas	Kansas	NNP
cord-293966-5c466xvz	215	17	.	.	.
cord-293966-5c466xvz	216	1	The	the	DT
cord-293966-5c466xvz	216	2	funders	funder	NNS
cord-293966-5c466xvz	216	3	had	have	VBD
cord-293966-5c466xvz	216	4	no	no	DT
cord-293966-5c466xvz	216	5	role	role	NN
cord-293966-5c466xvz	216	6	in	in	IN
cord-293966-5c466xvz	216	7	study	study	NN
cord-293966-5c466xvz	216	8	design	design	NN
cord-293966-5c466xvz	216	9	,	,	,
cord-293966-5c466xvz	216	10	data	datum	NNS
cord-293966-5c466xvz	216	11	collection	collection	NN
cord-293966-5c466xvz	216	12	and	and	CC
cord-293966-5c466xvz	216	13	interpretation	interpretation	NN
cord-293966-5c466xvz	216	14	,	,	,
cord-293966-5c466xvz	216	15	or	or	CC
cord-293966-5c466xvz	216	16	the	the	DT
cord-293966-5c466xvz	216	17	decision	decision	NN
cord-293966-5c466xvz	216	18	to	to	TO
cord-293966-5c466xvz	216	19	submit	submit	VB
cord-293966-5c466xvz	216	20	the	the	DT
cord-293966-5c466xvz	216	21	work	work	NN
cord-293966-5c466xvz	216	22	for	for	IN
cord-293966-5c466xvz	216	23	publication	publication	NN
cord-293966-5c466xvz	216	24	.	.	.