id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328361-hyrke6j2	Ithete, Ndapewa Laudika	Close Relative of Human Middle East Respiratory Syndrome Coronavirus in Bat, South Africa	2013-10-17		.txt	text/plain	1186	60	54	A novel clade 2c betacoronavirus, termed Middle East respiratory syndrome (MERS)-CoV, was recently identified as the causative agent of a severe respiratory disease that is mainly affecting humans on the Arabian Peninsula (1) . Extending on previous work (2), we described European Pipistrellus bat-derived CoVs that are closely related to MERS-CoV (3) . Screening for CoVs was done by nested reverse transcription PCR using broadly reactive oligonucleotide primers targeting a conserved region in the RNA-dependent RNA polymerase (RdRp) gene (online Technical Appendix). PCR amplicons for 4 positive specimens yielded alphacoronavirus sequences related to recently described bat alphacoronaviruses from South Africa (4) . A Bayesian phylogenetic analysis of the 816-nt RdRp sequence confirmed the close relationship between PML/2011 and MERS-CoV (Figure) . Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences	./cache/cord-328361-hyrke6j2.txt	./txt/cord-328361-hyrke6j2.txt