id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315909-vwugf0wp	Letko, Michael	Studying Evolutionary Adaptation of MERS-CoV	2019-09-14		.txt	text/plain	1236	100	52	Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018. Forced adaptation experiments have been used to determine viral mutations that facilitate escape from drugs [4] [5] [6] , monoclonal antibodies [7, 8] , host restriction factors [9] [10] [11] , and species variation in host receptors [12] [13] [14] and to elucidate various viral mechanisms of infection and replication [15] [16] [17] . Below is the method employed to adapt MERS-CoV to a semi-permissive host receptor, Desmodus rotundus DPP4. To increase selective pressures on a viral population which is beginning to show signs of adaptation, one can apply a population bottleneck in the subsequent passage by reducing the amount of viral Evolution of MERS-CoV supernatant passaged to the next cell culture.	./cache/cord-315909-vwugf0wp.txt	./txt/cord-315909-vwugf0wp.txt