id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-304030-6ve5plea	Aboagye, James Odame	Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10	2018-10-17		.txt	text/plain	3718	192	54	title: Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10 In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral–host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. The A549 transduced cells expressed relatively high MERS-N protein on both day 2 and 10 post-selection in an antibiotics medium as shown in Figure 2A . Fold regulations of antiviral response genes by MERS-N protein was compared with negative control cells, which were expressing LacZ, by using the 2 − C T method. Out of the nine host genes identified using stably transduced A549 cells, proinflammatory cytokine/chemokines TNF, IL6, IL8, and CXCL10 were reported to be up-regulated in MERS-CoV infected cells [3, 17, 20] . CXCL10 regulation has been reported in MERS patients [18, 19] as well as MERS-CoV infected cells [3, 17, 20] .	./cache/cord-304030-6ve5plea.txt	./txt/cord-304030-6ve5plea.txt