id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291199-nazl2e97	Kleine-Weber, Hannah	Mutations in the Spike Protein of Middle East Respiratory Syndrome Coronavirus Transmitted in Korea Increase Resistance to Antibody-Mediated Neutralization	2018-11-07		.txt	text/plain	5833	270	47	title: Mutations in the Spike Protein of Middle East Respiratory Syndrome Coronavirus Transmitted in Korea Increase Resistance to Antibody-Mediated Neutralization These findings indicate that MERS-CoV variants with reduced neutralization sensitivity were transmitted during the Korean outbreak and that the responsible mutations were compatible with robust infection of cells expressing high levels of DPP4. We demonstrate that these mutations reduce sensitivity to antibody-mediated neutralization and are compatible with robust infection of target cells expressing large amounts of the viral receptor DPP4. Incubation of control cells with MDL28170 reduced entry driven by MERS-S WT and S protein variants, and this effect was fully rescued by directed expression of TMPRSS2 (Fig. 5A ). Collectively, our findings and previous work suggest that MERS-CoV variants with at least partial resistance to antibody-mediated neutralization can retain high infectivity for cells expressing robust amounts of DPP4 and can spread between humans. Host cell entry of Middle East respiratory syndrome coronavirus after two-step, furin-mediated activation of the spike protein	./cache/cord-291199-nazl2e97.txt	./txt/cord-291199-nazl2e97.txt