id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-277823-vijh6x1l	TERAMICHI, Takurou	Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia	2019-11-05		.txt	text/plain	2247	107	52	A serological survey of Middle East respiratory syndrome coronavirus (MERS-CoV) was conducted among dromedary camels and herbivorous animals sharing the same pasturage in Ethiopia. One of camel serum that showed a high antibody titer in the neutralization test by live MERS-CoV was treated as a positive control. According to the results of the previous study, antibody titers of ≥16 are treated as positive in neutralization test using VSV-MERS/GFP. Cows that were antibody positive in the neutralization test using VSV-MERS/GFP or cELISA were different animals and both were antibody negative in the neutralization test using MERS-CoV. S1-ELISA was not sensitive compared to other tests because only 16 serum samples were positive and they required an antibody titer of ≥64 in VSV-MERS/GFP. The present study shows that the neutralization test using VSV-MERS/GFP, S1-ELISA, and cELISA are as specific to MERS-CoV infection as the serological tests, although their sensitivities slightly differ. Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study	./cache/cord-277823-vijh6x1l.txt	./txt/cord-277823-vijh6x1l.txt