id author title date pages extension mime words sentences flesch summary cache txt cord-272932-devmy5yx WANG, Wen Ling Serological Study of An Imported Case of Middle East Respiratory Syndrome and His Close Contacts in China, 2015 2016-03-31 .txt text/plain 2117 99 56 Moreover, no seroconversion was found among 53 close contacts by anti-MERS IgG antibody enzyme-linked immunosorbent assay (ELISA) of paired serum samples. To evaluate both the serological response of this MERS patient before discharge and the risk of transmission, a set of serum samples from the patient and paired serum samples collected at least 14 d apart from the close contacts of the MERS patient during his trip and hospital admission in China, were tested for MERS-CoV using an inactivated MERS-CoV-based ELISA. We used an inactivated MERS-CoV particle-based ELISA to analyze serum samples from the imported MERS-CoV patient and his close contacts for the presence of IgG against MERS-CoV. A lentivirus-based MERS-CoV pseudovirus neutralization test was performed to confirm the presence of MERS-CoV-specific antibodies in serum samples from the patient. All 53 paired serum samples from the close contacts were below the cut-off value, negative for MERS-CoV by ELISA (Figure 3 ). ./cache/cord-272932-devmy5yx.txt ./txt/cord-272932-devmy5yx.txt