id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261041-nrmj1qre	Algaissi, Abdullah	Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay	2019-09-14		.txt	text/plain	2578	205	69	However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). 5. Next day, change the media by removing old media and add 2 mL or 5 mL of fresh pre-warmed M-2 into a T25 or T175 tissue culture flask, respectively. 3. Seed 1 Â 10 4 Vero E6 cells (100 μL) per well into sterile 96-well tissue culture plate so that they are 90-95% confluent the next day (see Note 8). 3. Seed 1 Â 10 4 Vero E6 cells (100 μL) per well into sterile 96-well tissue culture plate so that they are 90-95% confluent the next day (see Note 8). Remove the 96-well tissue culture plate containing confluent Vero E6 cells and aspirate the media (see Note 15) .	./cache/cord-261041-nrmj1qre.txt	./txt/cord-261041-nrmj1qre.txt