id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259658-rgrt6e6r	Yan, Bingpeng	Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication	2019-01-16		.txt	text/plain	5299	287	41	To this end, we utilized the human coronavirus 229E (HCoV-229E) as a model coronavirus to comprehensively characterize the host cell lipid response upon coronavirus infection with an ultra-high performance liquid chromatography-mass spectrometry (UPLC–MS)-based lipidomics approach. Importantly, supplement of additional LA and AA to coronavirus-infected cells significantly inhibited virus replication of both HCoV-229E and the highly virulent MERS-CoV, suggesting that the LA-AA metabolism axis is a common and essential pathway that could modulate coronavirus replication. To investigate how coronavirus perturbs host lipid metabolism, we performed lipidomics analysis on HCoV-229E-infected Huh7 cells and compared the results with those of the mock-infected cells. Therefore, combining pathway analysis and the authentic standards verification results, our data suggested that the LA-AA metabolism axis was the most significantly perturbed pathway and might be associated with lipids rearrangement or other processes in HCoV-229E infection.	./cache/cord-259658-rgrt6e6r.txt	./txt/cord-259658-rgrt6e6r.txt