id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-257587-xjoyrdhj	Gunaratne, Gihan S.	A screening campaign in sea urchin egg homogenate as a platform for discovering modulators of NAADP-dependent Ca2+ signaling in human cells	2018-11-30		.txt	text/plain	7599	448	52	Here, we have performed a pilot screen for novel modulators of NAADP-sensitive Ca 2+ release in the sea urchin egg homogenate system and assessed tractability of the resulting 'hits' against both endogenous NAADP-evoked Ca 2+ responses and a pseudotyped MERS-CoV translocation assay in human cell lines [25] . Fangchinoline, an inhibitor of NAADPevoked Ca 2+ signals and MERS pseudovirus translocation in a human cell line [25] , decreased the magnitude of NAADP-evoked Ca 2+ release (peak amplitude 47 ± 2% of control response, blue traces in Fig. 1A ) with lesser effects on the size of IP 3 or cADPR-evoked Ca 2+ transients (Fig. 1A) . These final activities (steps '3' and '4') encompassed: (i) counter-screening for more generalized actions against acidic Ca 2+ stores, for example lysosomotropism [28, 29] , (ii) quantifying effects on NAADP-evoked Ca 2+ signals evoked by single cell microinjection of NAADP, and (iii) correlating effects on Ca 2+ release with bioactivities in the MERS pseudovirus translocation assay.	./cache/cord-257587-xjoyrdhj.txt	./txt/cord-257587-xjoyrdhj.txt