id	sid	tid	token	lemma	pos
cord-014597-66vd2mdu	1	1	key	key	JJ
cord-014597-66vd2mdu	2	1	:	:	:
cord-014597-66vd2mdu	2	2	cord-014597	cord-014597	NNP
cord-014597-66vd2mdu	2	3	-	-	HYPH
cord-014597-66vd2mdu	2	4	66vd2mdu	66vd2mdu	CD
cord-014597-66vd2mdu	2	5	authors	author	NNS
cord-014597-66vd2mdu	2	6	:	:	:
cord-014597-66vd2mdu	3	1	nan	nan	NNP
cord-014597-66vd2mdu	3	2	title	title	NN
cord-014597-66vd2mdu	3	3	:	:	:
cord-014597-66vd2mdu	3	4	Abstracts	abstract	NNS
cord-014597-66vd2mdu	3	5	from	from	IN
cord-014597-66vd2mdu	3	6	the	the	DT
cord-014597-66vd2mdu	3	7	25th	25th	JJ
cord-014597-66vd2mdu	3	8	European	European	NNP
cord-014597-66vd2mdu	3	9	Society	Society	NNP
cord-014597-66vd2mdu	3	10	for	for	IN
cord-014597-66vd2mdu	3	11	Animal	Animal	NNP
cord-014597-66vd2mdu	3	12	Cell	Cell	NNP
cord-014597-66vd2mdu	3	13	Technology	Technology	NNP
cord-014597-66vd2mdu	3	14	Meeting	meeting	NN
cord-014597-66vd2mdu	3	15	:	:	:
cord-014597-66vd2mdu	3	16	Cell	cell	NN
cord-014597-66vd2mdu	3	17	Technologies	Technologies	NNPS
cord-014597-66vd2mdu	3	18	for	for	IN
cord-014597-66vd2mdu	3	19	Innovative	Innovative	NNP
cord-014597-66vd2mdu	3	20	Therapies	Therapies	NNPS
cord-014597-66vd2mdu	3	21	:	:	:
cord-014597-66vd2mdu	3	22	Lausanne	Lausanne	NNP
cord-014597-66vd2mdu	3	23	,	,	,
cord-014597-66vd2mdu	3	24	Switzerland	Switzerland	NNP
cord-014597-66vd2mdu	3	25	.	.	.
cord-014597-66vd2mdu	4	1	14	14	CD
cord-014597-66vd2mdu	4	2	-	-	SYM
cord-014597-66vd2mdu	4	3	17	17	CD
cord-014597-66vd2mdu	4	4	May	May	NNP
cord-014597-66vd2mdu	4	5	2017	2017	CD
cord-014597-66vd2mdu	4	6	date	date	NN
cord-014597-66vd2mdu	4	7	:	:	:
cord-014597-66vd2mdu	4	8	2018	2018	CD
cord-014597-66vd2mdu	4	9	-	-	HYPH
cord-014597-66vd2mdu	4	10	03	03	CD
cord-014597-66vd2mdu	4	11	-	-	SYM
cord-014597-66vd2mdu	4	12	15	15	CD
cord-014597-66vd2mdu	4	13	journal	journal	NN
cord-014597-66vd2mdu	4	14	:	:	:
cord-014597-66vd2mdu	5	1	BMC	BMC	NNP
cord-014597-66vd2mdu	5	2	Proc	proc	NN
cord-014597-66vd2mdu	5	3	DOI	doi	NN
cord-014597-66vd2mdu	5	4	:	:	:
cord-014597-66vd2mdu	6	1	10.1186	10.1186	CD
cord-014597-66vd2mdu	6	2	/	/	SYM
cord-014597-66vd2mdu	6	3	s12919	s12919	NNP
cord-014597-66vd2mdu	6	4	-	-	HYPH
cord-014597-66vd2mdu	6	5	018	018	CD
cord-014597-66vd2mdu	6	6	-	-	HYPH
cord-014597-66vd2mdu	6	7	0097-x	0097-x	CD
cord-014597-66vd2mdu	7	1	sha	sha	NNP
cord-014597-66vd2mdu	7	2	:	:	:
cord-014597-66vd2mdu	8	1	c26196c940aab1a9c43e70d5428672644d1e0972	c26196c940aab1a9c43e70d5428672644d1e0972	NNP
cord-014597-66vd2mdu	8	2	doc_id	doc_id	CD
cord-014597-66vd2mdu	8	3	:	:	:
cord-014597-66vd2mdu	8	4	14597	14597	CD
cord-014597-66vd2mdu	8	5	cord_uid	cord_uid	NNS
cord-014597-66vd2mdu	8	6	:	:	:
cord-014597-66vd2mdu	9	1	66vd2mdu	66vd2mdu	CD
cord-014597-66vd2mdu	9	2	nan	nan	NNP
cord-014597-66vd2mdu	9	3	.	.	.
cord-014597-66vd2mdu	10	1	a	a	DT
cord-014597-66vd2mdu	10	2	Schematic	schematic	JJ
cord-014597-66vd2mdu	10	3	representation	representation	NN
cord-014597-66vd2mdu	10	4	of	of	IN
cord-014597-66vd2mdu	10	5	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	10	6	based	base	VBN
cord-014597-66vd2mdu	10	7	synthetic	synthetic	JJ
cord-014597-66vd2mdu	10	8	transcription	transcription	NN
cord-014597-66vd2mdu	10	9	factor	factor	NN
cord-014597-66vd2mdu	10	10	technology	technology	NN
cord-014597-66vd2mdu	10	11	.	.	.
cord-014597-66vd2mdu	11	1	b	b	LS
cord-014597-66vd2mdu	11	2	mRNA	mrna	NN
cord-014597-66vd2mdu	11	3	expression	expression	NN
cord-014597-66vd2mdu	11	4	levels	level	NNS
cord-014597-66vd2mdu	11	5	of	of	IN
cord-014597-66vd2mdu	11	6	protein	protein	NN
cord-014597-66vd2mdu	11	7	transport	transport	NN
cord-014597-66vd2mdu	11	8	related	relate	VBN
cord-014597-66vd2mdu	11	9	genes	gene	NNS
cord-014597-66vd2mdu	11	10	(	(	-LRB-
cord-014597-66vd2mdu	11	11	Napg	Napg	NNP
cord-014597-66vd2mdu	11	12	,	,	,
cord-014597-66vd2mdu	11	13	Rab5A	rab5a	NN
cord-014597-66vd2mdu	11	14	and	and	CC
cord-014597-66vd2mdu	11	15	Arpc1b	Arpc1b	NNP
cord-014597-66vd2mdu	11	16	)	)	-RRB-
cord-014597-66vd2mdu	11	17	.	.	.
cord-014597-66vd2mdu	12	1	Background	background	VB
cord-014597-66vd2mdu	12	2	An	an	DT
cord-014597-66vd2mdu	12	3	increasing	increase	VBG
cord-014597-66vd2mdu	12	4	number	number	NN
cord-014597-66vd2mdu	12	5	of	of	IN
cord-014597-66vd2mdu	12	6	biologics	biologic	NNS
cord-014597-66vd2mdu	12	7	are	be	VBP
cord-014597-66vd2mdu	12	8	entering	enter	VBG
cord-014597-66vd2mdu	12	9	the	the	DT
cord-014597-66vd2mdu	12	10	development	development	NN
cord-014597-66vd2mdu	12	11	pipelines	pipeline	NNS
cord-014597-66vd2mdu	12	12	of	of	IN
cord-014597-66vd2mdu	12	13	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	12	14	companies	company	NNS
cord-014597-66vd2mdu	12	15	[	[	-LRB-
cord-014597-66vd2mdu	12	16	1	1	CD
cord-014597-66vd2mdu	12	17	]	]	-RRB-
cord-014597-66vd2mdu	12	18	.	.	.
cord-014597-66vd2mdu	13	1	Today	today	NN
cord-014597-66vd2mdu	13	2	,	,	,
cord-014597-66vd2mdu	13	3	the	the	DT
cord-014597-66vd2mdu	13	4	preferred	preferred	JJ
cord-014597-66vd2mdu	13	5	production	production	NN
cord-014597-66vd2mdu	13	6	host	host	NN
cord-014597-66vd2mdu	13	7	for	for	IN
cord-014597-66vd2mdu	13	8	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	13	9	proteins	protein	NNS
cord-014597-66vd2mdu	13	10	is	be	VBZ
cord-014597-66vd2mdu	13	11	the	the	DT
cord-014597-66vd2mdu	13	12	CHO	CHO	NNP
cord-014597-66vd2mdu	13	13	cell	cell	NN
cord-014597-66vd2mdu	13	14	line	line	NN
cord-014597-66vd2mdu	13	15	.	.	.
cord-014597-66vd2mdu	14	1	However	however	RB
cord-014597-66vd2mdu	14	2	one	one	CD
cord-014597-66vd2mdu	14	3	of	of	IN
cord-014597-66vd2mdu	14	4	the	the	DT
cord-014597-66vd2mdu	14	5	major	major	JJ
cord-014597-66vd2mdu	14	6	hurdles	hurdle	NNS
cord-014597-66vd2mdu	14	7	,	,	,
cord-014597-66vd2mdu	14	8	especially	especially	RB
cord-014597-66vd2mdu	14	9	for	for	IN
cord-014597-66vd2mdu	14	10	the	the	DT
cord-014597-66vd2mdu	14	11	production	production	NN
cord-014597-66vd2mdu	14	12	of	of	IN
cord-014597-66vd2mdu	14	13	non	non	JJ
cord-014597-66vd2mdu	14	14	-	-	JJ
cord-014597-66vd2mdu	14	15	antibody	antibody	JJ
cord-014597-66vd2mdu	14	16	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	14	17	,	,	,
cord-014597-66vd2mdu	14	18	is	be	VBZ
cord-014597-66vd2mdu	14	19	host	host	NN
cord-014597-66vd2mdu	14	20	cell	cell	NN
cord-014597-66vd2mdu	14	21	-	-	HYPH
cord-014597-66vd2mdu	14	22	related	relate	VBN
cord-014597-66vd2mdu	14	23	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	14	24	degradation	degradation	NN
cord-014597-66vd2mdu	14	25	which	which	WDT
cord-014597-66vd2mdu	14	26	can	can	MD
cord-014597-66vd2mdu	14	27	drastically	drastically	RB
cord-014597-66vd2mdu	14	28	impact	impact	VB
cord-014597-66vd2mdu	14	29	developability	developability	NN
cord-014597-66vd2mdu	14	30	and	and	CC
cord-014597-66vd2mdu	14	31	timelines	timeline	NNS
cord-014597-66vd2mdu	14	32	of	of	IN
cord-014597-66vd2mdu	14	33	pipeline	pipeline	NN
cord-014597-66vd2mdu	14	34	projects	project	NNS
cord-014597-66vd2mdu	14	35	.	.	.
cord-014597-66vd2mdu	15	1	Material	material	NN
cord-014597-66vd2mdu	15	2	and	and	CC
cord-014597-66vd2mdu	15	3	methods	method	NNS
cord-014597-66vd2mdu	16	1	Spike	spike	NN
cord-014597-66vd2mdu	16	2	-	-	HYPH
cord-014597-66vd2mdu	16	3	in	in	NN
cord-014597-66vd2mdu	16	4	:	:	:
cord-014597-66vd2mdu	16	5	CHO	CHO	NNP
cord-014597-66vd2mdu	16	6	cells	cell	NNS
cord-014597-66vd2mdu	16	7	were	be	VBD
cord-014597-66vd2mdu	16	8	cultivated	cultivate	VBN
cord-014597-66vd2mdu	16	9	in	in	IN
cord-014597-66vd2mdu	16	10	a	a	DT
cord-014597-66vd2mdu	16	11	chemically	chemically	RB
cord-014597-66vd2mdu	16	12	defined	define	VBN
cord-014597-66vd2mdu	16	13	culture	culture	NN
cord-014597-66vd2mdu	16	14	medium	medium	NN
cord-014597-66vd2mdu	16	15	at	at	IN
cord-014597-66vd2mdu	16	16	36.5	36.5	CD
cord-014597-66vd2mdu	16	17	°	°	NN
cord-014597-66vd2mdu	16	18	C/10	C/10	NNP
cord-014597-66vd2mdu	16	19	%	%	NN
cord-014597-66vd2mdu	16	20	CO	CO	NNP
cord-014597-66vd2mdu	16	21	2	2	CD
cord-014597-66vd2mdu	16	22	in	in	IN
cord-014597-66vd2mdu	16	23	shake	shake	NN
cord-014597-66vd2mdu	16	24	-	-	HYPH
cord-014597-66vd2mdu	16	25	flasks	flask	NNS
cord-014597-66vd2mdu	16	26	.	.	.
cord-014597-66vd2mdu	17	1	When	when	WRB
cord-014597-66vd2mdu	17	2	the	the	DT
cord-014597-66vd2mdu	17	3	cells	cell	NNS
cord-014597-66vd2mdu	17	4	reached	reach	VBD
cord-014597-66vd2mdu	17	5	their	-PRON-	PRP$
cord-014597-66vd2mdu	17	6	maximum	maximum	JJ
cord-014597-66vd2mdu	17	7	viable	viable	JJ
cord-014597-66vd2mdu	17	8	density	density	NN
cord-014597-66vd2mdu	17	9	,	,	,
cord-014597-66vd2mdu	17	10	they	-PRON-	PRP
cord-014597-66vd2mdu	17	11	were	be	VBD
cord-014597-66vd2mdu	17	12	removed	remove	VBN
cord-014597-66vd2mdu	17	13	by	by	IN
cord-014597-66vd2mdu	17	14	centrifugation	centrifugation	NN
cord-014597-66vd2mdu	17	15	and	and	CC
cord-014597-66vd2mdu	17	16	the	the	DT
cord-014597-66vd2mdu	17	17	conditioned	condition	VBN
cord-014597-66vd2mdu	17	18	medium	medium	NN
cord-014597-66vd2mdu	17	19	was	be	VBD
cord-014597-66vd2mdu	17	20	collected	collect	VBN
cord-014597-66vd2mdu	17	21	.	.	.
cord-014597-66vd2mdu	18	1	A	a	DT
cord-014597-66vd2mdu	18	2	model	model	NN
cord-014597-66vd2mdu	18	3	mAb	mab	NN
cord-014597-66vd2mdu	18	4	was	be	VBD
cord-014597-66vd2mdu	18	5	spiked	spike	VBN
cord-014597-66vd2mdu	18	6	into	into	IN
cord-014597-66vd2mdu	18	7	the	the	DT
cord-014597-66vd2mdu	18	8	conditioned	condition	VBN
cord-014597-66vd2mdu	18	9	medium	medium	NN
cord-014597-66vd2mdu	18	10	and	and	CC
cord-014597-66vd2mdu	18	11	incubated	incubate	VBD
cord-014597-66vd2mdu	18	12	at	at	IN
cord-014597-66vd2mdu	18	13	37	37	CD
cord-014597-66vd2mdu	18	14	°	°	NN
cord-014597-66vd2mdu	18	15	C	c	NN
cord-014597-66vd2mdu	18	16	±	±	NNP
cord-014597-66vd2mdu	18	17	protease	protease	NN
cord-014597-66vd2mdu	18	18	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	18	19	.	.	.
cord-014597-66vd2mdu	19	1	The	the	DT
cord-014597-66vd2mdu	19	2	amount	amount	NN
cord-014597-66vd2mdu	19	3	of	of	IN
cord-014597-66vd2mdu	19	4	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	19	5	degradation	degradation	NN
cord-014597-66vd2mdu	19	6	was	be	VBD
cord-014597-66vd2mdu	19	7	analysed	analyse	VBN
cord-014597-66vd2mdu	19	8	by	by	IN
cord-014597-66vd2mdu	19	9	western	western	JJ
cord-014597-66vd2mdu	19	10	blot	blot	NN
cord-014597-66vd2mdu	19	11	and	and	CC
cord-014597-66vd2mdu	19	12	LC	LC	NNP
cord-014597-66vd2mdu	19	13	-	-	HYPH
cord-014597-66vd2mdu	19	14	MS	MS	NNP
cord-014597-66vd2mdu	19	15	.	.	.
cord-014597-66vd2mdu	20	1	Transcriptomics	transcriptomic	NNS
cord-014597-66vd2mdu	20	2	:	:	:
cord-014597-66vd2mdu	21	1	Total	Total	NNP
cord-014597-66vd2mdu	21	2	RNA	RNA	NNP
cord-014597-66vd2mdu	21	3	was	be	VBD
cord-014597-66vd2mdu	21	4	extracted	extract	VBN
cord-014597-66vd2mdu	21	5	after	after	IN
cord-014597-66vd2mdu	21	6	3	3	CD
cord-014597-66vd2mdu	21	7	days	day	NNS
cord-014597-66vd2mdu	21	8	of	of	IN
cord-014597-66vd2mdu	21	9	cell	cell	NN
cord-014597-66vd2mdu	21	10	cultivation	cultivation	NN
cord-014597-66vd2mdu	21	11	.	.	.
cord-014597-66vd2mdu	22	1	RNA	RNA	NNP
cord-014597-66vd2mdu	22	2	sequencing	sequencing	NN
cord-014597-66vd2mdu	22	3	libraries	library	NNS
cord-014597-66vd2mdu	22	4	were	be	VBD
cord-014597-66vd2mdu	22	5	constructed	construct	VBN
cord-014597-66vd2mdu	22	6	and	and	CC
cord-014597-66vd2mdu	22	7	processed	process	VBN
cord-014597-66vd2mdu	22	8	on	on	IN
cord-014597-66vd2mdu	22	9	the	the	DT
cord-014597-66vd2mdu	22	10	HiSeq	HiSeq	NNP
cord-014597-66vd2mdu	22	11	2000	2000	CD
cord-014597-66vd2mdu	22	12	platform	platform	NN
cord-014597-66vd2mdu	22	13	from	from	IN
cord-014597-66vd2mdu	22	14	Illumina	Illumina	NNP
cord-014597-66vd2mdu	22	15	.	.	.
cord-014597-66vd2mdu	23	1	Generation	generation	NN
cord-014597-66vd2mdu	23	2	of	of	IN
cord-014597-66vd2mdu	23	3	matriptase	matriptase	JJ
cord-014597-66vd2mdu	23	4	knockout	knockout	NN
cord-014597-66vd2mdu	23	5	:	:	:
cord-014597-66vd2mdu	23	6	CHO	CHO	NNP
cord-014597-66vd2mdu	23	7	-	-	HYPH
cord-014597-66vd2mdu	23	8	K1	K1	NNP
cord-014597-66vd2mdu	23	9	cells	cell	NNS
cord-014597-66vd2mdu	23	10	were	be	VBD
cord-014597-66vd2mdu	23	11	transfected	transfecte	VBN
cord-014597-66vd2mdu	23	12	with	with	IN
cord-014597-66vd2mdu	23	13	mRNA	mrna	NN
cord-014597-66vd2mdu	23	14	encoding	encode	VBG
cord-014597-66vd2mdu	23	15	"	"	''
cord-014597-66vd2mdu	23	16	transcription	transcription	NN
cord-014597-66vd2mdu	23	17	activator	activator	NN
cord-014597-66vd2mdu	23	18	-	-	HYPH
cord-014597-66vd2mdu	23	19	like	like	JJ
cord-014597-66vd2mdu	23	20	effector	effector	NN
cord-014597-66vd2mdu	23	21	nucleases	nuclease	NNS
cord-014597-66vd2mdu	23	22	"	"	''
cord-014597-66vd2mdu	23	23	or	or	CC
cord-014597-66vd2mdu	23	24	"	"	``
cord-014597-66vd2mdu	23	25	zinc	zinc	NN
cord-014597-66vd2mdu	23	26	finger	finger	NN
cord-014597-66vd2mdu	23	27	nucleases	nucleases	NNP
cord-014597-66vd2mdu	23	28	"	"	''
cord-014597-66vd2mdu	23	29	targeting	target	VBG
cord-014597-66vd2mdu	23	30	matriptase	matriptase	NNP
cord-014597-66vd2mdu	23	31	exon	exon	NNP
cord-014597-66vd2mdu	23	32	2	2	CD
cord-014597-66vd2mdu	23	33	.	.	.
cord-014597-66vd2mdu	24	1	The	the	DT
cord-014597-66vd2mdu	24	2	transfected	transfecte	VBN
cord-014597-66vd2mdu	24	3	cells	cell	NNS
cord-014597-66vd2mdu	24	4	were	be	VBD
cord-014597-66vd2mdu	24	5	subsequently	subsequently	RB
cord-014597-66vd2mdu	24	6	sorted	sort	VBN
cord-014597-66vd2mdu	24	7	into	into	IN
cord-014597-66vd2mdu	24	8	single	single	JJ
cord-014597-66vd2mdu	24	9	cells	cell	NNS
cord-014597-66vd2mdu	24	10	and	and	CC
cord-014597-66vd2mdu	24	11	analysed	analyse	VBN
cord-014597-66vd2mdu	24	12	for	for	IN
cord-014597-66vd2mdu	24	13	frameshift	frameshift	NN
cord-014597-66vd2mdu	24	14	mutations	mutation	NNS
cord-014597-66vd2mdu	24	15	in	in	IN
cord-014597-66vd2mdu	24	16	both	both	DT
cord-014597-66vd2mdu	24	17	alleles	allele	NNS
cord-014597-66vd2mdu	24	18	via	via	IN
cord-014597-66vd2mdu	24	19	Sanger	Sanger	NNP
cord-014597-66vd2mdu	24	20	sequencing	sequencing	NN
cord-014597-66vd2mdu	24	21	.	.	.
cord-014597-66vd2mdu	25	1	Cell	cell	NN
cord-014597-66vd2mdu	25	2	cultivation	cultivation	NN
cord-014597-66vd2mdu	25	3	:	:	:
cord-014597-66vd2mdu	25	4	Fed	Fed	NNP
cord-014597-66vd2mdu	25	5	batch	batch	NN
cord-014597-66vd2mdu	25	6	cultivation	cultivation	NN
cord-014597-66vd2mdu	25	7	was	be	VBD
cord-014597-66vd2mdu	25	8	performed	perform	VBN
cord-014597-66vd2mdu	25	9	in	in	IN
cord-014597-66vd2mdu	25	10	15-mL	15-ml	CD
cord-014597-66vd2mdu	25	11	miniaturized	miniaturized	JJ
cord-014597-66vd2mdu	25	12	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	25	13	(	(	-LRB-
cord-014597-66vd2mdu	25	14	AMBR15	AMBR15	NNP
cord-014597-66vd2mdu	25	15	)	)	-RRB-
cord-014597-66vd2mdu	25	16	.	.	.
cord-014597-66vd2mdu	26	1	Approximately	approximately	RB
cord-014597-66vd2mdu	26	2	700	700	CD
cord-014597-66vd2mdu	26	3	proteases	protease	NNS
cord-014597-66vd2mdu	26	4	are	be	VBP
cord-014597-66vd2mdu	26	5	known	know	VBN
cord-014597-66vd2mdu	26	6	in	in	IN
cord-014597-66vd2mdu	26	7	rodents	rodent	NNS
cord-014597-66vd2mdu	26	8	.	.	.
cord-014597-66vd2mdu	27	1	To	to	TO
cord-014597-66vd2mdu	27	2	reduce	reduce	VB
cord-014597-66vd2mdu	27	3	the	the	DT
cord-014597-66vd2mdu	27	4	number	number	NN
cord-014597-66vd2mdu	27	5	of	of	IN
cord-014597-66vd2mdu	27	6	candidate	candidate	NN
cord-014597-66vd2mdu	27	7	proteases	protease	NNS
cord-014597-66vd2mdu	27	8	we	-PRON-	PRP
cord-014597-66vd2mdu	27	9	showed	show	VBD
cord-014597-66vd2mdu	27	10	first	first	RB
cord-014597-66vd2mdu	27	11	that	that	IN
cord-014597-66vd2mdu	27	12	a	a	DT
cord-014597-66vd2mdu	27	13	model	model	NN
cord-014597-66vd2mdu	27	14	mAb	mAb	NNS
cord-014597-66vd2mdu	27	15	(	(	-LRB-
cord-014597-66vd2mdu	27	16	prone	prone	JJ
cord-014597-66vd2mdu	27	17	to	to	IN
cord-014597-66vd2mdu	27	18	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	27	19	degradation	degradation	NN
cord-014597-66vd2mdu	27	20	)	)	-RRB-
cord-014597-66vd2mdu	27	21	incubated	incubate	VBN
cord-014597-66vd2mdu	27	22	in	in	IN
cord-014597-66vd2mdu	27	23	conditioned	condition	VBN
cord-014597-66vd2mdu	27	24	medium	medium	NN
cord-014597-66vd2mdu	27	25	of	of	IN
cord-014597-66vd2mdu	27	26	CHO	CHO	NNP
cord-014597-66vd2mdu	27	27	-	-	HYPH
cord-014597-66vd2mdu	27	28	K1	K1	NNP
cord-014597-66vd2mdu	27	29	cells	cell	NNS
cord-014597-66vd2mdu	27	30	resulted	result	VBD
cord-014597-66vd2mdu	27	31	in	in	IN
cord-014597-66vd2mdu	27	32	clipping	clip	VBG
cord-014597-66vd2mdu	27	33	of	of	IN
cord-014597-66vd2mdu	27	34	the	the	DT
cord-014597-66vd2mdu	27	35	mAb	mAb	NNS
cord-014597-66vd2mdu	27	36	,	,	,
cord-014597-66vd2mdu	27	37	demonstrating	demonstrate	VBG
cord-014597-66vd2mdu	27	38	the	the	DT
cord-014597-66vd2mdu	27	39	involvement	involvement	NN
cord-014597-66vd2mdu	27	40	of	of	IN
cord-014597-66vd2mdu	27	41	secreted	secrete	VBN
cord-014597-66vd2mdu	27	42	/	/	SYM
cord-014597-66vd2mdu	27	43	shedded	shedde	VBN
cord-014597-66vd2mdu	27	44	proteases	protease	NNS
cord-014597-66vd2mdu	27	45	(	(	-LRB-
cord-014597-66vd2mdu	27	46	Fig	Fig	NNP
cord-014597-66vd2mdu	27	47	.	.	.
cord-014597-66vd2mdu	27	48	1a	1a	CD
cord-014597-66vd2mdu	27	49	)	)	-RRB-
cord-014597-66vd2mdu	27	50	.	.	.
cord-014597-66vd2mdu	28	1	Broad	broad	JJ
cord-014597-66vd2mdu	28	2	spectrum	spectrum	NN
cord-014597-66vd2mdu	28	3	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	28	4	of	of	IN
cord-014597-66vd2mdu	28	5	the	the	DT
cord-014597-66vd2mdu	28	6	different	different	JJ
cord-014597-66vd2mdu	28	7	protease	protease	NN
cord-014597-66vd2mdu	28	8	classes	class	NNS
cord-014597-66vd2mdu	28	9	revealed	reveal	VBD
cord-014597-66vd2mdu	28	10	that	that	IN
cord-014597-66vd2mdu	28	11	only	only	JJ
cord-014597-66vd2mdu	28	12	serine	serine	NN
cord-014597-66vd2mdu	28	13	protease	protease	NN
cord-014597-66vd2mdu	28	14	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	28	15	prevented	prevent	VBD
cord-014597-66vd2mdu	28	16	clipping	clip	VBG
cord-014597-66vd2mdu	28	17	.	.	.
cord-014597-66vd2mdu	29	1	Serine	serine	NN
cord-014597-66vd2mdu	29	2	protease	protease	NN
cord-014597-66vd2mdu	29	3	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	29	4	of	of	IN
cord-014597-66vd2mdu	29	5	higher	high	JJR
cord-014597-66vd2mdu	29	6	specificity	specificity	NN
cord-014597-66vd2mdu	29	7	highlighted	highlight	VBD
cord-014597-66vd2mdu	29	8	the	the	DT
cord-014597-66vd2mdu	29	9	group	group	NN
cord-014597-66vd2mdu	29	10	of	of	IN
cord-014597-66vd2mdu	29	11	"	"	``
cord-014597-66vd2mdu	29	12	S1A	s1a	NN
cord-014597-66vd2mdu	29	13	trypsin	trypsin	NN
cord-014597-66vd2mdu	29	14	-	-	HYPH
cord-014597-66vd2mdu	29	15	like	like	JJ
cord-014597-66vd2mdu	29	16	proteases	protease	NNS
cord-014597-66vd2mdu	29	17	"	"	''
cord-014597-66vd2mdu	29	18	(	(	-LRB-
cord-014597-66vd2mdu	29	19	Fig	fig	NN
cord-014597-66vd2mdu	29	20	.	.	.
cord-014597-66vd2mdu	29	21	1a	1a	CD
cord-014597-66vd2mdu	29	22	)	)	-RRB-
cord-014597-66vd2mdu	29	23	.	.	.
cord-014597-66vd2mdu	30	1	Comparison	comparison	NN
cord-014597-66vd2mdu	30	2	of	of	IN
cord-014597-66vd2mdu	30	3	the	the	DT
cord-014597-66vd2mdu	30	4	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	30	5	degradation	degradation	NN
cord-014597-66vd2mdu	30	6	profile	profile	NN
cord-014597-66vd2mdu	30	7	of	of	IN
cord-014597-66vd2mdu	30	8	several	several	JJ
cord-014597-66vd2mdu	30	9	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	30	10	proteins	protein	NNS
cord-014597-66vd2mdu	30	11	between	between	IN
cord-014597-66vd2mdu	30	12	CHO	CHO	NNP
cord-014597-66vd2mdu	30	13	-	-	HYPH
cord-014597-66vd2mdu	30	14	K1	K1	NNP
cord-014597-66vd2mdu	30	15	with	with	IN
cord-014597-66vd2mdu	30	16	another	another	DT
cord-014597-66vd2mdu	30	17	CHO	CHO	NNP
cord-014597-66vd2mdu	30	18	cell	cell	NN
cord-014597-66vd2mdu	30	19	line	line	NN
cord-014597-66vd2mdu	30	20	(	(	-LRB-
cord-014597-66vd2mdu	30	21	CHO	CHO	NNP
cord-014597-66vd2mdu	30	22	-	-	HYPH
cord-014597-66vd2mdu	30	23	A	A	NNP
cord-014597-66vd2mdu	30	24	)	)	-RRB-
cord-014597-66vd2mdu	30	25	revealed	reveal	VBN
cord-014597-66vd2mdu	30	26	less	less	JJR
cord-014597-66vd2mdu	30	27	degradation	degradation	NN
cord-014597-66vd2mdu	30	28	in	in	IN
cord-014597-66vd2mdu	31	1	CHO	CHO	NNP
cord-014597-66vd2mdu	31	2	-	-	HYPH
cord-014597-66vd2mdu	31	3	A.	A.	NNP
cord-014597-66vd2mdu	31	4	Therefore	Therefore	NNP
cord-014597-66vd2mdu	31	5	expression	expression	NN
cord-014597-66vd2mdu	31	6	of	of	IN
cord-014597-66vd2mdu	31	7	the	the	DT
cord-014597-66vd2mdu	31	8	involved	involved	JJ
cord-014597-66vd2mdu	31	9	protease(s	protease(s	NNP
cord-014597-66vd2mdu	31	10	)	)	-RRB-
cord-014597-66vd2mdu	31	11	is	be	VBZ
cord-014597-66vd2mdu	31	12	likely	likely	RB
cord-014597-66vd2mdu	31	13	lower	low	JJR
cord-014597-66vd2mdu	31	14	in	in	IN
cord-014597-66vd2mdu	31	15	CHO	CHO	NNP
cord-014597-66vd2mdu	31	16	-	-	HYPH
cord-014597-66vd2mdu	31	17	A.	A.	NNP
cord-014597-66vd2mdu	31	18	Gene	Gene	NNP
cord-014597-66vd2mdu	31	19	expression	expression	NN
cord-014597-66vd2mdu	31	20	profile	profile	NN
cord-014597-66vd2mdu	31	21	analysis	analysis	NN
cord-014597-66vd2mdu	31	22	of	of	IN
cord-014597-66vd2mdu	31	23	both	both	DT
cord-014597-66vd2mdu	31	24	cell	cell	NN
cord-014597-66vd2mdu	31	25	lines	line	NNS
cord-014597-66vd2mdu	32	1	showed	show	VBD
cord-014597-66vd2mdu	32	2	five	five	CD
cord-014597-66vd2mdu	32	3	secreted	secrete	VBN
cord-014597-66vd2mdu	32	4	/	/	SYM
cord-014597-66vd2mdu	32	5	shedded	shedde	VBD
cord-014597-66vd2mdu	32	6	"	"	``
cord-014597-66vd2mdu	32	7	S1A	s1a	NN
cord-014597-66vd2mdu	32	8	trypsin	trypsin	NN
cord-014597-66vd2mdu	32	9	-	-	HYPH
cord-014597-66vd2mdu	32	10	like	like	JJ
cord-014597-66vd2mdu	32	11	serine	serine	NN
cord-014597-66vd2mdu	32	12	proteases	protease	NNS
cord-014597-66vd2mdu	32	13	"	"	''
cord-014597-66vd2mdu	32	14	more	more	JJR
cord-014597-66vd2mdu	32	15	than	than	IN
cord-014597-66vd2mdu	32	16	1.5	1.5	CD
cord-014597-66vd2mdu	32	17	fold	fold	VB
cord-014597-66vd2mdu	32	18	lower	lower	RBR
cord-014597-66vd2mdu	32	19	expressed	express	VBN
cord-014597-66vd2mdu	32	20	in	in	IN
cord-014597-66vd2mdu	32	21	CHO	CHO	NNP
cord-014597-66vd2mdu	32	22	-	-	HYPH
cord-014597-66vd2mdu	32	23	CHO	CHO	NNP
cord-014597-66vd2mdu	32	24	-	-	HYPH
cord-014597-66vd2mdu	32	25	A	A	NNP
cord-014597-66vd2mdu	32	26	versus	versus	IN
cord-014597-66vd2mdu	32	27	CHO	CHO	NNP
cord-014597-66vd2mdu	32	28	-	-	HYPH
cord-014597-66vd2mdu	32	29	K1	K1	NNP
cord-014597-66vd2mdu	32	30	(	(	-LRB-
cord-014597-66vd2mdu	32	31	Fig	Fig	NNP
cord-014597-66vd2mdu	32	32	.	.	.
cord-014597-66vd2mdu	32	33	1b	1b	CD
cord-014597-66vd2mdu	32	34	)	)	-RRB-
cord-014597-66vd2mdu	32	35	.	.	.
cord-014597-66vd2mdu	33	1	Surprisingly	surprisingly	RB
cord-014597-66vd2mdu	33	2	,	,	,
cord-014597-66vd2mdu	33	3	siRNA	siRNA	NNP
cord-014597-66vd2mdu	33	4	knockdown	knockdown	NN
cord-014597-66vd2mdu	33	5	experiments	experiment	NNS
cord-014597-66vd2mdu	33	6	of	of	IN
cord-014597-66vd2mdu	33	7	these	these	DT
cord-014597-66vd2mdu	33	8	five	five	CD
cord-014597-66vd2mdu	33	9	candidates	candidate	NNS
cord-014597-66vd2mdu	33	10	identified	identify	VBD
cord-014597-66vd2mdu	33	11	"	"	``
cord-014597-66vd2mdu	33	12	Matriptase	Matriptase	NNP
cord-014597-66vd2mdu	33	13	"	"	''
cord-014597-66vd2mdu	33	14	as	as	IN
cord-014597-66vd2mdu	33	15	the	the	DT
cord-014597-66vd2mdu	33	16	major	major	JJ
cord-014597-66vd2mdu	33	17	protease	protease	NN
cord-014597-66vd2mdu	33	18	involved	involve	VBN
cord-014597-66vd2mdu	33	19	in	in	IN
cord-014597-66vd2mdu	33	20	degradation	degradation	NN
cord-014597-66vd2mdu	33	21	of	of	IN
cord-014597-66vd2mdu	33	22	recombinant	recombinant	JJ
cord-014597-66vd2mdu	33	23	proteins	protein	NNS
cord-014597-66vd2mdu	33	24	expressed	express	VBN
cord-014597-66vd2mdu	33	25	in	in	IN
cord-014597-66vd2mdu	33	26	CHO	CHO	NNP
cord-014597-66vd2mdu	33	27	-	-	HYPH
cord-014597-66vd2mdu	33	28	K1	K1	NNP
cord-014597-66vd2mdu	33	29	cells	cell	NNS
cord-014597-66vd2mdu	33	30	(	(	-LRB-
cord-014597-66vd2mdu	33	31	Fig	fig	NN
cord-014597-66vd2mdu	33	32	.	.	.
cord-014597-66vd2mdu	34	1	1c	1c	NNP
cord-014597-66vd2mdu	34	2	upper	upper	NNP
cord-014597-66vd2mdu	34	3	panel	panel	NN
cord-014597-66vd2mdu	34	4	)	)	-RRB-
cord-014597-66vd2mdu	34	5	.	.	.
cord-014597-66vd2mdu	35	1	Next	next	RB
cord-014597-66vd2mdu	35	2	,	,	,
cord-014597-66vd2mdu	35	3	we	-PRON-	PRP
cord-014597-66vd2mdu	35	4	generated	generate	VBD
cord-014597-66vd2mdu	35	5	a	a	DT
cord-014597-66vd2mdu	35	6	CHO	CHO	NNP
cord-014597-66vd2mdu	35	7	-	-	HYPH
cord-014597-66vd2mdu	35	8	K1	K1	NNP
cord-014597-66vd2mdu	35	9	matriptase	matriptase	NN
cord-014597-66vd2mdu	35	10	knockout	knockout	NN
cord-014597-66vd2mdu	35	11	(	(	-LRB-
cord-014597-66vd2mdu	35	12	KO	KO	NNP
cord-014597-66vd2mdu	35	13	)	)	-RRB-
cord-014597-66vd2mdu	35	14	cell	cell	NN
cord-014597-66vd2mdu	35	15	line	line	NN
cord-014597-66vd2mdu	35	16	.	.	.
cord-014597-66vd2mdu	36	1	No	no	DT
cord-014597-66vd2mdu	36	2	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	36	3	degradation	degradation	NN
cord-014597-66vd2mdu	36	4	product	product	NN
cord-014597-66vd2mdu	36	5	was	be	VBD
cord-014597-66vd2mdu	36	6	detected	detect	VBN
cord-014597-66vd2mdu	36	7	when	when	WRB
cord-014597-66vd2mdu	36	8	the	the	DT
cord-014597-66vd2mdu	36	9	model	model	NN
cord-014597-66vd2mdu	36	10	mAb	mAb	NNP
cord-014597-66vd2mdu	36	11	was	be	VBD
cord-014597-66vd2mdu	36	12	spiked	spike	VBN
cord-014597-66vd2mdu	36	13	into	into	IN
cord-014597-66vd2mdu	36	14	conditioned	condition	VBN
cord-014597-66vd2mdu	36	15	medium	medium	NN
cord-014597-66vd2mdu	36	16	of	of	IN
cord-014597-66vd2mdu	36	17	the	the	DT
cord-014597-66vd2mdu	36	18	KO	KO	NNP
cord-014597-66vd2mdu	36	19	cell	cell	NN
cord-014597-66vd2mdu	36	20	line	line	NN
cord-014597-66vd2mdu	36	21	(	(	-LRB-
cord-014597-66vd2mdu	36	22	Fig	Fig	NNP
cord-014597-66vd2mdu	36	23	.	.	.
cord-014597-66vd2mdu	36	24	1c	1c	NNP
cord-014597-66vd2mdu	36	25	lower	low	JJR
cord-014597-66vd2mdu	36	26	panel	panel	NN
cord-014597-66vd2mdu	36	27	)	)	-RRB-
cord-014597-66vd2mdu	36	28	.	.	.
cord-014597-66vd2mdu	37	1	Also	also	RB
cord-014597-66vd2mdu	37	2	,	,	,
cord-014597-66vd2mdu	37	3	stable	stable	JJ
cord-014597-66vd2mdu	37	4	expression	expression	NN
cord-014597-66vd2mdu	37	5	of	of	IN
cord-014597-66vd2mdu	37	6	the	the	DT
cord-014597-66vd2mdu	37	7	model	model	NN
cord-014597-66vd2mdu	37	8	mAb	mab	NN
cord-014597-66vd2mdu	37	9	in	in	IN
cord-014597-66vd2mdu	37	10	the	the	DT
cord-014597-66vd2mdu	37	11	KO	KO	NNP
cord-014597-66vd2mdu	37	12	cell	cell	NN
cord-014597-66vd2mdu	37	13	line	line	NN
cord-014597-66vd2mdu	37	14	resulted	result	VBD
cord-014597-66vd2mdu	37	15	in	in	IN
cord-014597-66vd2mdu	37	16	no	no	DT
cord-014597-66vd2mdu	37	17	/	/	SYM
cord-014597-66vd2mdu	37	18	significantly	significantly	RB
cord-014597-66vd2mdu	37	19	less	less	JJR
cord-014597-66vd2mdu	37	20	clipping	clip	VBG
cord-014597-66vd2mdu	37	21	(	(	-LRB-
cord-014597-66vd2mdu	37	22	Fig	Fig	NNP
cord-014597-66vd2mdu	37	23	.	.	.
cord-014597-66vd2mdu	37	24	1e	1e	LS
cord-014597-66vd2mdu	37	25	)	)	-RRB-
cord-014597-66vd2mdu	37	26	.	.	.
cord-014597-66vd2mdu	38	1	The	the	DT
cord-014597-66vd2mdu	38	2	protein	protein	NN
cord-014597-66vd2mdu	38	3	titer	titer	NN
cord-014597-66vd2mdu	38	4	and	and	CC
cord-014597-66vd2mdu	38	5	the	the	DT
cord-014597-66vd2mdu	38	6	cell	cell	NN
cord-014597-66vd2mdu	38	7	growth	growth	NN
cord-014597-66vd2mdu	38	8	behaviour	behaviour	NN
cord-014597-66vd2mdu	38	9	of	of	IN
cord-014597-66vd2mdu	38	10	the	the	DT
cord-014597-66vd2mdu	38	11	matriptase	matriptase	NNP
cord-014597-66vd2mdu	38	12	KO	KO	NNP
cord-014597-66vd2mdu	38	13	cells	cell	NNS
cord-014597-66vd2mdu	38	14	were	be	VBD
cord-014597-66vd2mdu	38	15	similar	similar	JJ
cord-014597-66vd2mdu	38	16	to	to	IN
cord-014597-66vd2mdu	38	17	the	the	DT
cord-014597-66vd2mdu	38	18	corresponding	correspond	VBG
cord-014597-66vd2mdu	38	19	wildtype	wildtype	NN
cord-014597-66vd2mdu	38	20	(	(	-LRB-
cord-014597-66vd2mdu	38	21	wt	wt	NN
cord-014597-66vd2mdu	38	22	)	)	-RRB-
cord-014597-66vd2mdu	38	23	cells	cell	NNS
cord-014597-66vd2mdu	38	24	(	(	-LRB-
cord-014597-66vd2mdu	38	25	Fig	Fig	NNP
cord-014597-66vd2mdu	38	26	.	.	.
cord-014597-66vd2mdu	38	27	1d	1d	LS
cord-014597-66vd2mdu	38	28	)	)	-RRB-
cord-014597-66vd2mdu	38	29	as	as	IN
cord-014597-66vd2mdu	38	30	shown	show	VBN
cord-014597-66vd2mdu	38	31	by	by	IN
cord-014597-66vd2mdu	38	32	comparative	comparative	JJ
cord-014597-66vd2mdu	38	33	cultivation	cultivation	NN
cord-014597-66vd2mdu	38	34	in	in	IN
cord-014597-66vd2mdu	38	35	AMBR	AMBR	NNP
cord-014597-66vd2mdu	38	36	system	system	NN
cord-014597-66vd2mdu	38	37	.	.	.
cord-014597-66vd2mdu	39	1	Conclusions	conclusion	NNS
cord-014597-66vd2mdu	40	1	One	one	CD
cord-014597-66vd2mdu	40	2	major	major	JJ
cord-014597-66vd2mdu	40	3	challenge	challenge	NN
cord-014597-66vd2mdu	40	4	for	for	IN
cord-014597-66vd2mdu	40	5	the	the	DT
cord-014597-66vd2mdu	40	6	production	production	NN
cord-014597-66vd2mdu	40	7	of	of	IN
cord-014597-66vd2mdu	40	8	recombinant	recombinant	JJ
cord-014597-66vd2mdu	40	9	proteins	protein	NNS
cord-014597-66vd2mdu	40	10	is	be	VBZ
cord-014597-66vd2mdu	40	11	CHO	CHO	NNP
cord-014597-66vd2mdu	40	12	host	host	NN
cord-014597-66vd2mdu	40	13	cell	cell	NN
cord-014597-66vd2mdu	40	14	mediated	mediate	VBN
cord-014597-66vd2mdu	40	15	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	40	16	degradation	degradation	NN
cord-014597-66vd2mdu	40	17	which	which	WDT
cord-014597-66vd2mdu	40	18	can	can	MD
cord-014597-66vd2mdu	40	19	negatively	negatively	RB
cord-014597-66vd2mdu	40	20	impact	impact	VB
cord-014597-66vd2mdu	40	21	or	or	CC
cord-014597-66vd2mdu	40	22	even	even	RB
cord-014597-66vd2mdu	40	23	result	result	VB
cord-014597-66vd2mdu	40	24	in	in	IN
cord-014597-66vd2mdu	40	25	termination	termination	NN
cord-014597-66vd2mdu	40	26	of	of	IN
cord-014597-66vd2mdu	40	27	projects	project	NNS
cord-014597-66vd2mdu	40	28	[	[	-LRB-
cord-014597-66vd2mdu	40	29	2	2	CD
cord-014597-66vd2mdu	40	30	;	;	:
cord-014597-66vd2mdu	40	31	3	3	CD
cord-014597-66vd2mdu	40	32	]	]	-RRB-
cord-014597-66vd2mdu	40	33	.	.	.
cord-014597-66vd2mdu	41	1	Using	use	VBG
cord-014597-66vd2mdu	41	2	a	a	DT
cord-014597-66vd2mdu	41	3	variety	variety	NN
cord-014597-66vd2mdu	41	4	of	of	IN
cord-014597-66vd2mdu	41	5	techniques	technique	NNS
cord-014597-66vd2mdu	41	6	such	such	JJ
cord-014597-66vd2mdu	41	7	as	as	IN
cord-014597-66vd2mdu	41	8	applying	apply	VBG
cord-014597-66vd2mdu	41	9	protease	protease	NN
cord-014597-66vd2mdu	41	10	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	41	11	,	,	,
cord-014597-66vd2mdu	41	12	transcriptomics	transcriptomics	NNP
cord-014597-66vd2mdu	41	13	and	and	CC
cord-014597-66vd2mdu	41	14	siRNA	siRNA	NNP
cord-014597-66vd2mdu	41	15	mediated	mediate	VBD
cord-014597-66vd2mdu	41	16	knock	knock	NN
cord-014597-66vd2mdu	41	17	-	-	HYPH
cord-014597-66vd2mdu	41	18	down	down	RP
cord-014597-66vd2mdu	41	19	we	-PRON-	PRP
cord-014597-66vd2mdu	41	20	were	be	VBD
cord-014597-66vd2mdu	41	21	able	able	JJ
cord-014597-66vd2mdu	41	22	to	to	TO
cord-014597-66vd2mdu	41	23	identify	identify	VB
cord-014597-66vd2mdu	41	24	"	"	``
cord-014597-66vd2mdu	41	25	matriptase	matriptase	VB
cord-014597-66vd2mdu	41	26	"	"	''
cord-014597-66vd2mdu	41	27	as	as	IN
cord-014597-66vd2mdu	41	28	the	the	DT
cord-014597-66vd2mdu	41	29	major	major	JJ
cord-014597-66vd2mdu	41	30	protease	protease	NN
cord-014597-66vd2mdu	41	31	involved	involve	VBN
cord-014597-66vd2mdu	41	32	in	in	IN
cord-014597-66vd2mdu	41	33	degradation	degradation	NN
cord-014597-66vd2mdu	41	34	of	of	IN
cord-014597-66vd2mdu	41	35	recombinant	recombinant	JJ
cord-014597-66vd2mdu	41	36	proteins	protein	NNS
cord-014597-66vd2mdu	41	37	expressed	express	VBN
cord-014597-66vd2mdu	41	38	in	in	IN
cord-014597-66vd2mdu	41	39	CHO	CHO	NNP
cord-014597-66vd2mdu	41	40	-	-	HYPH
cord-014597-66vd2mdu	41	41	K1	K1	NNP
cord-014597-66vd2mdu	41	42	cells	cell	NNS
cord-014597-66vd2mdu	41	43	.	.	.
cord-014597-66vd2mdu	42	1	Subsequently	subsequently	RB
cord-014597-66vd2mdu	42	2	we	-PRON-	PRP
cord-014597-66vd2mdu	42	3	generated	generate	VBD
cord-014597-66vd2mdu	42	4	a	a	DT
cord-014597-66vd2mdu	42	5	matriptase	matriptase	JJ
cord-014597-66vd2mdu	42	6	deficient	deficient	JJ
cord-014597-66vd2mdu	42	7	CHO	CHO	NNP
cord-014597-66vd2mdu	42	8	cell	cell	NN
cord-014597-66vd2mdu	42	9	line	line	NN
cord-014597-66vd2mdu	42	10	.	.	.
cord-014597-66vd2mdu	43	1	Protein	protein	NN
cord-014597-66vd2mdu	43	2	candidates	candidate	NNS
cord-014597-66vd2mdu	43	3	of	of	IN
cord-014597-66vd2mdu	43	4	diverse	diverse	JJ
cord-014597-66vd2mdu	43	5	formats	format	NNS
cord-014597-66vd2mdu	43	6	,	,	,
cord-014597-66vd2mdu	43	7	severely	severely	RB
cord-014597-66vd2mdu	43	8	degraded	degrade	VBN
cord-014597-66vd2mdu	43	9	in	in	IN
cord-014597-66vd2mdu	43	10	wt	wt	NN
cord-014597-66vd2mdu	43	11	CHO	CHO	NNP
cord-014597-66vd2mdu	43	12	-	-	HYPH
cord-014597-66vd2mdu	43	13	K1	K1	NNP
cord-014597-66vd2mdu	43	14	cell	cell	NN
cord-014597-66vd2mdu	43	15	line	line	NN
cord-014597-66vd2mdu	43	16	,	,	,
cord-014597-66vd2mdu	43	17	were	be	VBD
cord-014597-66vd2mdu	43	18	not	not	RB
cord-014597-66vd2mdu	43	19	or	or	CC
cord-014597-66vd2mdu	43	20	significantly	significantly	RB
cord-014597-66vd2mdu	43	21	less	less	RBR
cord-014597-66vd2mdu	43	22	cleaved	cleave	VBN
cord-014597-66vd2mdu	43	23	in	in	IN
cord-014597-66vd2mdu	43	24	the	the	DT
cord-014597-66vd2mdu	43	25	matriptase	matriptase	NN
cord-014597-66vd2mdu	43	26	KO	KO	NNP
cord-014597-66vd2mdu	43	27	cell	cell	NN
cord-014597-66vd2mdu	43	28	line	line	NN
cord-014597-66vd2mdu	43	29	.	.	.
cord-014597-66vd2mdu	44	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	44	2	cell	cell	NN
cord-014597-66vd2mdu	44	3	growth	growth	NN
cord-014597-66vd2mdu	44	4	,	,	,
cord-014597-66vd2mdu	44	5	viability	viability	NN
cord-014597-66vd2mdu	44	6	and	and	CC
cord-014597-66vd2mdu	44	7	productivity	productivity	NN
cord-014597-66vd2mdu	44	8	levels	level	NNS
cord-014597-66vd2mdu	44	9	were	be	VBD
cord-014597-66vd2mdu	44	10	comparable	comparable	JJ
cord-014597-66vd2mdu	44	11	between	between	IN
cord-014597-66vd2mdu	44	12	the	the	DT
cord-014597-66vd2mdu	44	13	wt	wt	NN
cord-014597-66vd2mdu	44	14	and	and	CC
cord-014597-66vd2mdu	44	15	the	the	DT
cord-014597-66vd2mdu	44	16	matriptase	matriptase	NNP
cord-014597-66vd2mdu	44	17	KO	KO	NNP
cord-014597-66vd2mdu	44	18	cell	cell	NN
cord-014597-66vd2mdu	44	19	line	line	NN
cord-014597-66vd2mdu	44	20	.	.	.
cord-014597-66vd2mdu	45	1	In	in	IN
cord-014597-66vd2mdu	45	2	summary	summary	NN
cord-014597-66vd2mdu	45	3	,	,	,
cord-014597-66vd2mdu	45	4	we	-PRON-	PRP
cord-014597-66vd2mdu	45	5	have	have	VBP
cord-014597-66vd2mdu	45	6	generated	generate	VBN
cord-014597-66vd2mdu	45	7	a	a	DT
cord-014597-66vd2mdu	45	8	superior	superior	JJ
cord-014597-66vd2mdu	45	9	platform	platform	NN
cord-014597-66vd2mdu	45	10	-	-	HYPH
cord-014597-66vd2mdu	45	11	compatible	compatible	JJ
cord-014597-66vd2mdu	45	12	CHO	CHO	NNP
cord-014597-66vd2mdu	45	13	production	production	NN
cord-014597-66vd2mdu	45	14	host	host	NN
cord-014597-66vd2mdu	45	15	cell	cell	NN
cord-014597-66vd2mdu	45	16	line	line	NN
cord-014597-66vd2mdu	45	17	with	with	IN
cord-014597-66vd2mdu	45	18	the	the	DT
cord-014597-66vd2mdu	45	19	same	same	JJ
cord-014597-66vd2mdu	45	20	favourable	favourable	JJ
cord-014597-66vd2mdu	45	21	productivity	productivity	NN
cord-014597-66vd2mdu	45	22	properties	property	NNS
cord-014597-66vd2mdu	45	23	as	as	IN
cord-014597-66vd2mdu	45	24	the	the	DT
cord-014597-66vd2mdu	45	25	parental	parental	JJ
cord-014597-66vd2mdu	45	26	host	host	NN
cord-014597-66vd2mdu	45	27	cell	cell	NN
cord-014597-66vd2mdu	45	28	line	line	NN
cord-014597-66vd2mdu	45	29	[	[	-LRB-
cord-014597-66vd2mdu	45	30	4	4	CD
cord-014597-66vd2mdu	45	31	;	;	SYM
cord-014597-66vd2mdu	45	32	6	6	CD
cord-014597-66vd2mdu	45	33	]	]	-RRB-
cord-014597-66vd2mdu	45	34	,	,	,
cord-014597-66vd2mdu	45	35	allowing	allow	VBG
cord-014597-66vd2mdu	45	36	expression	expression	NN
cord-014597-66vd2mdu	45	37	of	of	IN
cord-014597-66vd2mdu	45	38	complex	complex	JJ
cord-014597-66vd2mdu	45	39	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	45	40	prone	prone	JJ
cord-014597-66vd2mdu	45	41	to	to	IN
cord-014597-66vd2mdu	45	42	clipping	clip	VBG
cord-014597-66vd2mdu	45	43	.	.	.
cord-014597-66vd2mdu	46	1	Background	Background	NNP
cord-014597-66vd2mdu	46	2	CHO	CHO	NNP
cord-014597-66vd2mdu	46	3	cell	cell	NN
cord-014597-66vd2mdu	46	4	lines	line	NNS
cord-014597-66vd2mdu	46	5	are	be	VBP
cord-014597-66vd2mdu	46	6	common	common	JJ
cord-014597-66vd2mdu	46	7	hosts	host	NNS
cord-014597-66vd2mdu	46	8	for	for	IN
cord-014597-66vd2mdu	46	9	the	the	DT
cord-014597-66vd2mdu	46	10	production	production	NN
cord-014597-66vd2mdu	46	11	of	of	IN
cord-014597-66vd2mdu	46	12	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	46	13	proteins	protein	NNS
cord-014597-66vd2mdu	46	14	.	.	.
cord-014597-66vd2mdu	47	1	So	so	RB
cord-014597-66vd2mdu	47	2	far	far	RB
cord-014597-66vd2mdu	47	3	,	,	,
cord-014597-66vd2mdu	47	4	considerable	considerable	JJ
cord-014597-66vd2mdu	47	5	progress	progress	NN
cord-014597-66vd2mdu	47	6	has	have	VBZ
cord-014597-66vd2mdu	47	7	been	be	VBN
cord-014597-66vd2mdu	47	8	made	make	VBN
cord-014597-66vd2mdu	47	9	increasing	increase	VBG
cord-014597-66vd2mdu	47	10	productivity	productivity	NN
cord-014597-66vd2mdu	47	11	of	of	IN
cord-014597-66vd2mdu	47	12	cell	cell	NN
cord-014597-66vd2mdu	47	13	culture	culture	NN
cord-014597-66vd2mdu	47	14	to	to	TO
cord-014597-66vd2mdu	47	15	meet	meet	VB
cord-014597-66vd2mdu	47	16	the	the	DT
cord-014597-66vd2mdu	47	17	rapidly	rapidly	RB
cord-014597-66vd2mdu	47	18	growing	grow	VBG
cord-014597-66vd2mdu	47	19	demand	demand	NN
cord-014597-66vd2mdu	47	20	for	for	IN
cord-014597-66vd2mdu	47	21	antibody	antibody	NN
cord-014597-66vd2mdu	47	22	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	47	23	through	through	IN
cord-014597-66vd2mdu	47	24	increased	increase	VBN
cord-014597-66vd2mdu	47	25	cell	cell	NN
cord-014597-66vd2mdu	47	26	densities	density	NNS
cord-014597-66vd2mdu	47	27	and	and	CC
cord-014597-66vd2mdu	47	28	longer	long	JJR
cord-014597-66vd2mdu	47	29	culture	culture	NN
cord-014597-66vd2mdu	47	30	times	time	NNS
cord-014597-66vd2mdu	47	31	.	.	.
cord-014597-66vd2mdu	48	1	The	the	DT
cord-014597-66vd2mdu	48	2	downside	downside	NN
cord-014597-66vd2mdu	48	3	is	be	VBZ
cord-014597-66vd2mdu	48	4	the	the	DT
cord-014597-66vd2mdu	48	5	increase	increase	NN
cord-014597-66vd2mdu	48	6	of	of	IN
cord-014597-66vd2mdu	48	7	the	the	DT
cord-014597-66vd2mdu	48	8	process	process	NN
cord-014597-66vd2mdu	48	9	related	relate	VBN
cord-014597-66vd2mdu	48	10	impurities	impurity	NNS
cord-014597-66vd2mdu	48	11	,	,	,
cord-014597-66vd2mdu	48	12	bringing	bring	VBG
cord-014597-66vd2mdu	48	13	new	new	JJ
cord-014597-66vd2mdu	48	14	challenges	challenge	NNS
cord-014597-66vd2mdu	48	15	for	for	IN
cord-014597-66vd2mdu	48	16	process	process	NN
cord-014597-66vd2mdu	48	17	and	and	CC
cord-014597-66vd2mdu	48	18	harvest	harvest	NN
cord-014597-66vd2mdu	48	19	development	development	NN
cord-014597-66vd2mdu	48	20	.	.	.
cord-014597-66vd2mdu	49	1	Among	among	IN
cord-014597-66vd2mdu	49	2	the	the	DT
cord-014597-66vd2mdu	49	3	process	process	NN
cord-014597-66vd2mdu	49	4	related	relate	VBN
cord-014597-66vd2mdu	49	5	impurities	impurity	NNS
cord-014597-66vd2mdu	49	6	such	such	JJ
cord-014597-66vd2mdu	49	7	as	as	IN
cord-014597-66vd2mdu	49	8	host	host	NN
cord-014597-66vd2mdu	49	9	cell	cell	NN
cord-014597-66vd2mdu	49	10	proteins	protein	NNS
cord-014597-66vd2mdu	49	11	(	(	-LRB-
cord-014597-66vd2mdu	49	12	HCPs	HCPs	NNP
cord-014597-66vd2mdu	49	13	)	)	-RRB-
cord-014597-66vd2mdu	49	14	or	or	CC
cord-014597-66vd2mdu	49	15	DNA	dna	VB
cord-014597-66vd2mdu	49	16	the	the	DT
cord-014597-66vd2mdu	49	17	potential	potential	JJ
cord-014597-66vd2mdu	49	18	impact	impact	NN
cord-014597-66vd2mdu	49	19	of	of	IN
cord-014597-66vd2mdu	49	20	lipids	lipid	NNS
cord-014597-66vd2mdu	49	21	production	production	NN
cord-014597-66vd2mdu	49	22	and	and	CC
cord-014597-66vd2mdu	49	23	release	release	NN
cord-014597-66vd2mdu	49	24	during	during	IN
cord-014597-66vd2mdu	49	25	cell	cell	NN
cord-014597-66vd2mdu	49	26	culture	culture	NN
cord-014597-66vd2mdu	49	27	is	be	VBZ
cord-014597-66vd2mdu	49	28	still	still	RB
cord-014597-66vd2mdu	49	29	poorly	poorly	RB
cord-014597-66vd2mdu	49	30	understood	understand	VBN
cord-014597-66vd2mdu	49	31	due	due	IN
cord-014597-66vd2mdu	49	32	to	to	IN
cord-014597-66vd2mdu	49	33	the	the	DT
cord-014597-66vd2mdu	49	34	complex	complex	JJ
cord-014597-66vd2mdu	49	35	nature	nature	NN
cord-014597-66vd2mdu	49	36	and	and	CC
cord-014597-66vd2mdu	49	37	diversity	diversity	NN
cord-014597-66vd2mdu	49	38	of	of	IN
cord-014597-66vd2mdu	49	39	this	this	DT
cord-014597-66vd2mdu	49	40	class	class	NN
cord-014597-66vd2mdu	49	41	of	of	IN
cord-014597-66vd2mdu	49	42	molecules	molecule	NNS
cord-014597-66vd2mdu	49	43	.	.	.
cord-014597-66vd2mdu	50	1	Thanks	thank	NNS
cord-014597-66vd2mdu	50	2	to	to	IN
cord-014597-66vd2mdu	50	3	recent	recent	JJ
cord-014597-66vd2mdu	50	4	advances	advance	NNS
cord-014597-66vd2mdu	50	5	in	in	IN
cord-014597-66vd2mdu	50	6	analytical	analytical	JJ
cord-014597-66vd2mdu	50	7	tools	tool	NNS
cord-014597-66vd2mdu	50	8	especially	especially	RB
cord-014597-66vd2mdu	50	9	mass	mass	JJ
cord-014597-66vd2mdu	50	10	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	50	11	,	,	,
cord-014597-66vd2mdu	50	12	the	the	DT
cord-014597-66vd2mdu	50	13	advent	advent	NN
cord-014597-66vd2mdu	50	14	of	of	IN
cord-014597-66vd2mdu	50	15	lipidomics	lipidomics	NNP
cord-014597-66vd2mdu	50	16	offers	offer	VBZ
cord-014597-66vd2mdu	50	17	now	now	RB
cord-014597-66vd2mdu	50	18	the	the	DT
cord-014597-66vd2mdu	50	19	feasibility	feasibility	NN
cord-014597-66vd2mdu	50	20	to	to	TO
cord-014597-66vd2mdu	50	21	study	study	VB
cord-014597-66vd2mdu	50	22	several	several	JJ
cord-014597-66vd2mdu	50	23	thousands	thousand	NNS
cord-014597-66vd2mdu	50	24	of	of	IN
cord-014597-66vd2mdu	50	25	lipid	lipid	NN
cord-014597-66vd2mdu	50	26	species	specie	NNS
cord-014597-66vd2mdu	50	27	thus	thus	RB
cord-014597-66vd2mdu	50	28	unraveling	unravel	VBG
cord-014597-66vd2mdu	50	29	the	the	DT
cord-014597-66vd2mdu	50	30	possibility	possibility	NN
cord-014597-66vd2mdu	50	31	to	to	TO
cord-014597-66vd2mdu	50	32	understand	understand	VB
cord-014597-66vd2mdu	50	33	and	and	CC
cord-014597-66vd2mdu	50	34	potentially	potentially	RB
cord-014597-66vd2mdu	50	35	control	control	VB
cord-014597-66vd2mdu	50	36	the	the	DT
cord-014597-66vd2mdu	50	37	interactions	interaction	NNS
cord-014597-66vd2mdu	50	38	between	between	IN
cord-014597-66vd2mdu	50	39	high	high	JJ
cord-014597-66vd2mdu	50	40	performance	performance	NN
cord-014597-66vd2mdu	50	41	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	50	42	processes	process	NNS
cord-014597-66vd2mdu	50	43	,	,	,
cord-014597-66vd2mdu	50	44	harvest	harvest	NN
cord-014597-66vd2mdu	50	45	conditions	condition	NNS
cord-014597-66vd2mdu	50	46	and	and	CC
cord-014597-66vd2mdu	50	47	purification	purification	NN
cord-014597-66vd2mdu	50	48	.	.	.
cord-014597-66vd2mdu	51	1	In	in	IN
cord-014597-66vd2mdu	51	2	order	order	NN
cord-014597-66vd2mdu	51	3	to	to	TO
cord-014597-66vd2mdu	51	4	analyze	analyze	VB
cord-014597-66vd2mdu	51	5	and	and	CC
cord-014597-66vd2mdu	51	6	quantify	quantify	VB
cord-014597-66vd2mdu	51	7	lipids	lipid	NNS
cord-014597-66vd2mdu	51	8	,	,	,
cord-014597-66vd2mdu	51	9	we	-PRON-	PRP
cord-014597-66vd2mdu	51	10	developed	develop	VBD
cord-014597-66vd2mdu	51	11	a	a	DT
cord-014597-66vd2mdu	51	12	three	three	CD
cord-014597-66vd2mdu	51	13	steps	step	NNS
cord-014597-66vd2mdu	51	14	method	method	NN
cord-014597-66vd2mdu	51	15	.	.	.
cord-014597-66vd2mdu	52	1	In	in	IN
cord-014597-66vd2mdu	52	2	a	a	DT
cord-014597-66vd2mdu	52	3	first	first	JJ
cord-014597-66vd2mdu	52	4	step	step	NN
cord-014597-66vd2mdu	52	5	,	,	,
cord-014597-66vd2mdu	52	6	lipids	lipid	NNS
cord-014597-66vd2mdu	52	7	were	be	VBD
cord-014597-66vd2mdu	52	8	extracted	extract	VBN
cord-014597-66vd2mdu	52	9	with	with	IN
cord-014597-66vd2mdu	52	10	Methyl	Methyl	NNP
cord-014597-66vd2mdu	52	11	tert	tert	NN
cord-014597-66vd2mdu	52	12	-	-	HYPH
cord-014597-66vd2mdu	52	13	butyl	butyl	NN
cord-014597-66vd2mdu	52	14	ether	ether	NN
cord-014597-66vd2mdu	52	15	(	(	-LRB-
cord-014597-66vd2mdu	52	16	MTBE	MTBE	NNP
cord-014597-66vd2mdu	52	17	)	)	-RRB-
cord-014597-66vd2mdu	52	18	according	accord	VBG
cord-014597-66vd2mdu	52	19	to	to	IN
cord-014597-66vd2mdu	52	20	Matyash	Matyash	NNP
cord-014597-66vd2mdu	52	21	method	method	NN
cord-014597-66vd2mdu	52	22	[	[	-LRB-
cord-014597-66vd2mdu	52	23	1	1	CD
cord-014597-66vd2mdu	52	24	]	]	-RRB-
cord-014597-66vd2mdu	52	25	.	.	.
cord-014597-66vd2mdu	53	1	Lipids	lipid	NNS
cord-014597-66vd2mdu	53	2	were	be	VBD
cord-014597-66vd2mdu	53	3	then	then	RB
cord-014597-66vd2mdu	53	4	separated	separate	VBN
cord-014597-66vd2mdu	53	5	by	by	IN
cord-014597-66vd2mdu	53	6	liquid	liquid	JJ
cord-014597-66vd2mdu	53	7	chromatography	chromatography	NN
cord-014597-66vd2mdu	53	8	using	use	VBG
cord-014597-66vd2mdu	53	9	either	either	CC
cord-014597-66vd2mdu	53	10	HILIC	HILIC	NNP
cord-014597-66vd2mdu	53	11	of	of	IN
cord-014597-66vd2mdu	53	12	reverse	reverse	JJ
cord-014597-66vd2mdu	53	13	phase	phase	NN
cord-014597-66vd2mdu	53	14	column	column	NN
cord-014597-66vd2mdu	53	15	prior	prior	RB
cord-014597-66vd2mdu	53	16	to	to	IN
cord-014597-66vd2mdu	53	17	detection	detection	NN
cord-014597-66vd2mdu	53	18	and	and	CC
cord-014597-66vd2mdu	53	19	quantification	quantification	NN
cord-014597-66vd2mdu	53	20	by	by	IN
cord-014597-66vd2mdu	53	21	mass	mass	NN
cord-014597-66vd2mdu	53	22	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	53	23	.	.	.
cord-014597-66vd2mdu	54	1	All	all	DT
cord-014597-66vd2mdu	54	2	lipid	lipid	NN
cord-014597-66vd2mdu	54	3	classes	class	NNS
cord-014597-66vd2mdu	54	4	were	be	VBD
cord-014597-66vd2mdu	54	5	detected	detect	VBN
cord-014597-66vd2mdu	54	6	by	by	IN
cord-014597-66vd2mdu	54	7	ESI	ESI	NNP
cord-014597-66vd2mdu	54	8	-	-	HYPH
cord-014597-66vd2mdu	54	9	MS	MS	NNP
cord-014597-66vd2mdu	54	10	/	/	SYM
cord-014597-66vd2mdu	54	11	MS	MS	NNP
cord-014597-66vd2mdu	54	12	excepted	except	VBD
cord-014597-66vd2mdu	54	13	cholesterol	cholesterol	NN
cord-014597-66vd2mdu	54	14	(	(	-LRB-
cord-014597-66vd2mdu	54	15	APCI	APCI	NNP
cord-014597-66vd2mdu	54	16	-	-	HYPH
cord-014597-66vd2mdu	54	17	MS	MS	NNP
cord-014597-66vd2mdu	54	18	/	/	SYM
cord-014597-66vd2mdu	54	19	MS	MS	NNP
cord-014597-66vd2mdu	54	20	)	)	-RRB-
cord-014597-66vd2mdu	54	21	.	.	.
cord-014597-66vd2mdu	55	1	Finally	finally	RB
cord-014597-66vd2mdu	55	2	we	-PRON-	PRP
cord-014597-66vd2mdu	55	3	applied	apply	VBD
cord-014597-66vd2mdu	55	4	this	this	DT
cord-014597-66vd2mdu	55	5	method	method	NN
cord-014597-66vd2mdu	55	6	to	to	TO
cord-014597-66vd2mdu	55	7	analyze	analyze	VB
cord-014597-66vd2mdu	55	8	the	the	DT
cord-014597-66vd2mdu	55	9	lipid	lipid	NN
cord-014597-66vd2mdu	55	10	content	content	NN
cord-014597-66vd2mdu	55	11	of	of	IN
cord-014597-66vd2mdu	55	12	different	different	JJ
cord-014597-66vd2mdu	55	13	cell	cell	NN
cord-014597-66vd2mdu	55	14	lines	line	NNS
cord-014597-66vd2mdu	55	15	each	each	DT
cord-014597-66vd2mdu	55	16	expressing	express	VBG
cord-014597-66vd2mdu	55	17	a	a	DT
cord-014597-66vd2mdu	55	18	different	different	JJ
cord-014597-66vd2mdu	55	19	recombinant	recombinant	JJ
cord-014597-66vd2mdu	55	20	protein	protein	NN
cord-014597-66vd2mdu	55	21	,	,	,
cord-014597-66vd2mdu	55	22	during	during	IN
cord-014597-66vd2mdu	55	23	a	a	DT
cord-014597-66vd2mdu	55	24	14	14	CD
cord-014597-66vd2mdu	55	25	days	day	NNS
cord-014597-66vd2mdu	55	26	fed	feed	VBN
cord-014597-66vd2mdu	55	27	batch	batch	NN
cord-014597-66vd2mdu	55	28	process	process	NN
cord-014597-66vd2mdu	55	29	.	.	.
cord-014597-66vd2mdu	56	1	Lipid	lipid	NN
cord-014597-66vd2mdu	56	2	from	from	IN
cord-014597-66vd2mdu	56	3	CHO	CHO	NNP
cord-014597-66vd2mdu	56	4	cells	cell	NNS
cord-014597-66vd2mdu	56	5	were	be	VBD
cord-014597-66vd2mdu	56	6	successfully	successfully	RB
cord-014597-66vd2mdu	56	7	extracted	extract	VBN
cord-014597-66vd2mdu	56	8	with	with	IN
cord-014597-66vd2mdu	56	9	a	a	DT
cord-014597-66vd2mdu	56	10	yield	yield	NN
cord-014597-66vd2mdu	56	11	between	between	IN
cord-014597-66vd2mdu	56	12	80	80	CD
cord-014597-66vd2mdu	56	13	%	%	NN
cord-014597-66vd2mdu	56	14	and	and	CC
cord-014597-66vd2mdu	56	15	95	95	CD
cord-014597-66vd2mdu	56	16	%	%	NN
cord-014597-66vd2mdu	56	17	depending	depend	VBG
cord-014597-66vd2mdu	56	18	on	on	IN
cord-014597-66vd2mdu	56	19	the	the	DT
cord-014597-66vd2mdu	56	20	different	different	JJ
cord-014597-66vd2mdu	56	21	lipid	lipid	NN
cord-014597-66vd2mdu	56	22	classes	class	NNS
cord-014597-66vd2mdu	56	23	.	.	.
cord-014597-66vd2mdu	57	1	Stable	stable	JJ
cord-014597-66vd2mdu	57	2	isotope	isotope	NN
cord-014597-66vd2mdu	57	3	labeled	label	VBN
cord-014597-66vd2mdu	57	4	lipids	lipid	NNS
cord-014597-66vd2mdu	57	5	were	be	VBD
cord-014597-66vd2mdu	57	6	used	use	VBN
cord-014597-66vd2mdu	57	7	as	as	IN
cord-014597-66vd2mdu	57	8	internal	internal	JJ
cord-014597-66vd2mdu	57	9	standard	standard	NN
cord-014597-66vd2mdu	57	10	in	in	IN
cord-014597-66vd2mdu	57	11	order	order	NN
cord-014597-66vd2mdu	57	12	to	to	TO
cord-014597-66vd2mdu	57	13	have	have	VB
cord-014597-66vd2mdu	57	14	comparable	comparable	JJ
cord-014597-66vd2mdu	57	15	results	result	NNS
cord-014597-66vd2mdu	57	16	between	between	IN
cord-014597-66vd2mdu	57	17	batches	batch	NNS
cord-014597-66vd2mdu	57	18	.	.	.
cord-014597-66vd2mdu	58	1	The	the	DT
cord-014597-66vd2mdu	58	2	obtained	obtain	VBN
cord-014597-66vd2mdu	58	3	results	result	NNS
cord-014597-66vd2mdu	58	4	(	(	-LRB-
cord-014597-66vd2mdu	58	5	Fig	fig	NN
cord-014597-66vd2mdu	58	6	.	.	.
cord-014597-66vd2mdu	58	7	1	1	LS
cord-014597-66vd2mdu	58	8	)	)	-RRB-
cord-014597-66vd2mdu	58	9	show	show	VBP
cord-014597-66vd2mdu	58	10	that	that	IN
cord-014597-66vd2mdu	58	11	for	for	IN
cord-014597-66vd2mdu	58	12	a	a	DT
cord-014597-66vd2mdu	58	13	given	give	VBN
cord-014597-66vd2mdu	58	14	cell	cell	NN
cord-014597-66vd2mdu	58	15	line	line	NN
cord-014597-66vd2mdu	58	16	,	,	,
cord-014597-66vd2mdu	58	17	lipid	lipid	NN
cord-014597-66vd2mdu	58	18	distribution	distribution	NN
cord-014597-66vd2mdu	58	19	is	be	VBZ
cord-014597-66vd2mdu	58	20	changing	change	VBG
cord-014597-66vd2mdu	58	21	over	over	IN
cord-014597-66vd2mdu	58	22	the	the	DT
cord-014597-66vd2mdu	58	23	process	process	NN
cord-014597-66vd2mdu	58	24	.	.	.
cord-014597-66vd2mdu	59	1	Moreover	moreover	RB
cord-014597-66vd2mdu	59	2	,	,	,
cord-014597-66vd2mdu	59	3	this	this	DT
cord-014597-66vd2mdu	59	4	distribution	distribution	NN
cord-014597-66vd2mdu	59	5	may	may	MD
cord-014597-66vd2mdu	59	6	vary	vary	VB
cord-014597-66vd2mdu	59	7	significantly	significantly	RB
cord-014597-66vd2mdu	59	8	depending	depend	VBG
cord-014597-66vd2mdu	59	9	on	on	IN
cord-014597-66vd2mdu	59	10	the	the	DT
cord-014597-66vd2mdu	59	11	cell	cell	NN
cord-014597-66vd2mdu	59	12	line	line	NN
cord-014597-66vd2mdu	59	13	:	:	:
cord-014597-66vd2mdu	60	1	CL-1	CL-1	NNP
cord-014597-66vd2mdu	60	2	and	and	CC
cord-014597-66vd2mdu	60	3	in	in	IN
cord-014597-66vd2mdu	60	4	a	a	DT
cord-014597-66vd2mdu	60	5	lower	low	JJR
cord-014597-66vd2mdu	60	6	extend	extend	NN
cord-014597-66vd2mdu	60	7	,	,	,
cord-014597-66vd2mdu	60	8	CL-3	CL-3	NNP
cord-014597-66vd2mdu	60	9	,	,	,
cord-014597-66vd2mdu	60	10	show	show	VBP
cord-014597-66vd2mdu	60	11	an	an	DT
cord-014597-66vd2mdu	60	12	accumulation	accumulation	NN
cord-014597-66vd2mdu	60	13	of	of	IN
cord-014597-66vd2mdu	60	14	triglycerides	triglyceride	NNS
cord-014597-66vd2mdu	60	15	from	from	IN
cord-014597-66vd2mdu	60	16	day	day	NN
cord-014597-66vd2mdu	60	17	6	6	CD
cord-014597-66vd2mdu	60	18	to	to	IN
cord-014597-66vd2mdu	60	19	the	the	DT
cord-014597-66vd2mdu	60	20	end	end	NN
cord-014597-66vd2mdu	60	21	of	of	IN
cord-014597-66vd2mdu	60	22	the	the	DT
cord-014597-66vd2mdu	60	23	process	process	NN
cord-014597-66vd2mdu	60	24	,	,	,
cord-014597-66vd2mdu	60	25	while	while	IN
cord-014597-66vd2mdu	60	26	CL-2	CL-2	NNP
cord-014597-66vd2mdu	60	27	does	do	VBZ
cord-014597-66vd2mdu	60	28	n't	not	RB
cord-014597-66vd2mdu	60	29	seems	seem	VBZ
cord-014597-66vd2mdu	60	30	to	to	TO
cord-014597-66vd2mdu	60	31	follow	follow	VB
cord-014597-66vd2mdu	60	32	this	this	DT
cord-014597-66vd2mdu	60	33	trend	trend	NN
cord-014597-66vd2mdu	60	34	.	.	.
cord-014597-66vd2mdu	61	1	Conclusion	conclusion	NN
cord-014597-66vd2mdu	61	2	Interestingly	interestingly	RB
cord-014597-66vd2mdu	61	3	,	,	,
cord-014597-66vd2mdu	61	4	in	in	IN
cord-014597-66vd2mdu	61	5	some	some	DT
cord-014597-66vd2mdu	61	6	cell	cell	NN
cord-014597-66vd2mdu	61	7	lines	line	NNS
cord-014597-66vd2mdu	61	8	/	/	SYM
cord-014597-66vd2mdu	61	9	experimental	experimental	JJ
cord-014597-66vd2mdu	61	10	conditions	condition	NNS
cord-014597-66vd2mdu	61	11	,	,	,
cord-014597-66vd2mdu	61	12	we	-PRON-	PRP
cord-014597-66vd2mdu	61	13	highlighted	highlight	VBD
cord-014597-66vd2mdu	61	14	an	an	DT
cord-014597-66vd2mdu	61	15	overproduction	overproduction	NN
cord-014597-66vd2mdu	61	16	of	of	IN
cord-014597-66vd2mdu	61	17	triglycerides	triglyceride	NNS
cord-014597-66vd2mdu	61	18	and	and	CC
cord-014597-66vd2mdu	61	19	cholesterol	cholesterol	NN
cord-014597-66vd2mdu	61	20	leading	lead	VBG
cord-014597-66vd2mdu	61	21	to	to	IN
cord-014597-66vd2mdu	61	22	the	the	DT
cord-014597-66vd2mdu	61	23	accumulation	accumulation	NN
cord-014597-66vd2mdu	61	24	of	of	IN
cord-014597-66vd2mdu	61	25	lipid	lipid	NN
cord-014597-66vd2mdu	61	26	droplets	droplet	NNS
cord-014597-66vd2mdu	61	27	known	know	VBN
cord-014597-66vd2mdu	61	28	as	as	IN
cord-014597-66vd2mdu	61	29	energy	energy	NN
cord-014597-66vd2mdu	61	30	storage	storage	NN
cord-014597-66vd2mdu	61	31	sink	sink	NN
cord-014597-66vd2mdu	61	32	.	.	.
cord-014597-66vd2mdu	62	1	At	at	IN
cord-014597-66vd2mdu	62	2	the	the	DT
cord-014597-66vd2mdu	62	3	metabolic	metabolic	JJ
cord-014597-66vd2mdu	62	4	level	level	NN
cord-014597-66vd2mdu	62	5	,	,	,
cord-014597-66vd2mdu	62	6	these	these	DT
cord-014597-66vd2mdu	62	7	findings	finding	NNS
cord-014597-66vd2mdu	62	8	suggest	suggest	VBP
cord-014597-66vd2mdu	62	9	a	a	DT
cord-014597-66vd2mdu	62	10	relative	relative	JJ
cord-014597-66vd2mdu	62	11	overflow	overflow	NN
cord-014597-66vd2mdu	62	12	of	of	IN
cord-014597-66vd2mdu	62	13	the	the	DT
cord-014597-66vd2mdu	62	14	carbon	carbon	NN
cord-014597-66vd2mdu	62	15	metabolism	metabolism	NN
cord-014597-66vd2mdu	62	16	.	.	.
cord-014597-66vd2mdu	63	1	From	from	IN
cord-014597-66vd2mdu	63	2	a	a	DT
cord-014597-66vd2mdu	63	3	process	process	NN
cord-014597-66vd2mdu	63	4	development	development	NN
cord-014597-66vd2mdu	63	5	perspective	perspective	NN
cord-014597-66vd2mdu	63	6	these	these	DT
cord-014597-66vd2mdu	63	7	findings	finding	NNS
cord-014597-66vd2mdu	63	8	can	can	MD
cord-014597-66vd2mdu	63	9	be	be	VB
cord-014597-66vd2mdu	63	10	considered	consider	VBN
cord-014597-66vd2mdu	63	11	on	on	IN
cord-014597-66vd2mdu	63	12	the	the	DT
cord-014597-66vd2mdu	63	13	one	one	CD
cord-014597-66vd2mdu	63	14	hand	hand	NN
cord-014597-66vd2mdu	63	15	as	as	IN
cord-014597-66vd2mdu	63	16	a	a	DT
cord-014597-66vd2mdu	63	17	resource	resource	NN
cord-014597-66vd2mdu	63	18	waste	waste	NN
cord-014597-66vd2mdu	63	19	since	since	IN
cord-014597-66vd2mdu	63	20	the	the	DT
cord-014597-66vd2mdu	63	21	stored	store	VBN
cord-014597-66vd2mdu	63	22	energy	energy	NN
cord-014597-66vd2mdu	63	23	is	be	VBZ
cord-014597-66vd2mdu	63	24	not	not	RB
cord-014597-66vd2mdu	63	25	used	use	VBN
cord-014597-66vd2mdu	63	26	for	for	IN
cord-014597-66vd2mdu	63	27	protein	protein	NN
cord-014597-66vd2mdu	63	28	/	/	SYM
cord-014597-66vd2mdu	63	29	biomass	biomass	NN
cord-014597-66vd2mdu	63	30	biosynthesis	biosynthesis	NN
cord-014597-66vd2mdu	63	31	and	and	CC
cord-014597-66vd2mdu	63	32	on	on	IN
cord-014597-66vd2mdu	63	33	the	the	DT
cord-014597-66vd2mdu	63	34	second	second	JJ
cord-014597-66vd2mdu	63	35	hand	hand	NN
cord-014597-66vd2mdu	63	36	as	as	IN
cord-014597-66vd2mdu	63	37	the	the	DT
cord-014597-66vd2mdu	63	38	root	root	NN
cord-014597-66vd2mdu	63	39	cause	cause	NN
cord-014597-66vd2mdu	63	40	of	of	IN
cord-014597-66vd2mdu	63	41	additional	additional	JJ
cord-014597-66vd2mdu	63	42	process	process	NN
cord-014597-66vd2mdu	63	43	challenges	challenge	NNS
cord-014597-66vd2mdu	63	44	especially	especially	RB
cord-014597-66vd2mdu	63	45	during	during	IN
cord-014597-66vd2mdu	63	46	the	the	DT
cord-014597-66vd2mdu	63	47	harvest	harvest	NN
cord-014597-66vd2mdu	63	48	and	and	CC
cord-014597-66vd2mdu	63	49	the	the	DT
cord-014597-66vd2mdu	63	50	first	first	JJ
cord-014597-66vd2mdu	63	51	capture	capture	NN
cord-014597-66vd2mdu	63	52	steps	step	NNS
cord-014597-66vd2mdu	63	53	given	give	VBN
cord-014597-66vd2mdu	63	54	the	the	DT
cord-014597-66vd2mdu	63	55	hydrophobic	hydrophobic	JJ
cord-014597-66vd2mdu	63	56	nature	nature	NN
cord-014597-66vd2mdu	63	57	of	of	IN
cord-014597-66vd2mdu	63	58	these	these	DT
cord-014597-66vd2mdu	63	59	molecules	molecule	NNS
cord-014597-66vd2mdu	63	60	.	.	.
cord-014597-66vd2mdu	64	1	Implementation	implementation	NN
cord-014597-66vd2mdu	64	2	of	of	IN
cord-014597-66vd2mdu	64	3	lipidomics	lipidomic	NNS
cord-014597-66vd2mdu	64	4	analysis	analysis	NN
cord-014597-66vd2mdu	64	5	enables	enable	VBZ
cord-014597-66vd2mdu	64	6	us	-PRON-	PRP
cord-014597-66vd2mdu	64	7	to	to	TO
cord-014597-66vd2mdu	64	8	highlight	highlight	VB
cord-014597-66vd2mdu	64	9	a	a	DT
cord-014597-66vd2mdu	64	10	new	new	JJ
cord-014597-66vd2mdu	64	11	type	type	NN
cord-014597-66vd2mdu	64	12	of	of	IN
cord-014597-66vd2mdu	64	13	process	process	NN
cord-014597-66vd2mdu	64	14	variability	variability	NN
cord-014597-66vd2mdu	64	15	and	and	CC
cord-014597-66vd2mdu	64	16	to	to	TO
cord-014597-66vd2mdu	64	17	anticipate	anticipate	VB
cord-014597-66vd2mdu	64	18	potential	potential	JJ
cord-014597-66vd2mdu	64	19	problems	problem	NNS
cord-014597-66vd2mdu	64	20	for	for	IN
cord-014597-66vd2mdu	64	21	the	the	DT
cord-014597-66vd2mdu	64	22	downstream	downstream	JJ
cord-014597-66vd2mdu	64	23	steps	step	NNS
cord-014597-66vd2mdu	64	24	.	.	.
cord-014597-66vd2mdu	65	1	The	the	DT
cord-014597-66vd2mdu	65	2	application	application	NN
cord-014597-66vd2mdu	65	3	of	of	IN
cord-014597-66vd2mdu	65	4	this	this	DT
cord-014597-66vd2mdu	65	5	methodology	methodology	NN
cord-014597-66vd2mdu	65	6	on	on	IN
cord-014597-66vd2mdu	65	7	our	-PRON-	PRP$
cord-014597-66vd2mdu	65	8	platform	platform	NN
cord-014597-66vd2mdu	65	9	has	have	VBZ
cord-014597-66vd2mdu	65	10	helped	help	VBN
cord-014597-66vd2mdu	65	11	us	-PRON-	PRP
cord-014597-66vd2mdu	65	12	to	to	TO
cord-014597-66vd2mdu	65	13	design	design	VB
cord-014597-66vd2mdu	65	14	tailor	tailor	NN
cord-014597-66vd2mdu	65	15	made	make	VBD
cord-014597-66vd2mdu	65	16	solutions	solution	NNS
cord-014597-66vd2mdu	65	17	(	(	-LRB-
cord-014597-66vd2mdu	65	18	pretreatment	pretreatment	NN
cord-014597-66vd2mdu	65	19	selection	selection	NN
cord-014597-66vd2mdu	65	20	,	,	,
cord-014597-66vd2mdu	65	21	filter	filter	NN
cord-014597-66vd2mdu	65	22	selection	selection	NN
cord-014597-66vd2mdu	65	23	,	,	,
cord-014597-66vd2mdu	65	24	…	…	NFP
cord-014597-66vd2mdu	65	25	)	)	-RRB-
cord-014597-66vd2mdu	65	26	at	at	IN
cord-014597-66vd2mdu	65	27	the	the	DT
cord-014597-66vd2mdu	65	28	clarification	clarification	NN
cord-014597-66vd2mdu	65	29	step	step	NN
cord-014597-66vd2mdu	65	30	which	which	WDT
cord-014597-66vd2mdu	65	31	are	be	VBP
cord-014597-66vd2mdu	65	32	now	now	RB
cord-014597-66vd2mdu	65	33	implemented	implement	VBN
cord-014597-66vd2mdu	65	34	in	in	IN
cord-014597-66vd2mdu	65	35	our	-PRON-	PRP$
cord-014597-66vd2mdu	65	36	harvest	harvest	NN
cord-014597-66vd2mdu	65	37	development	development	NN
cord-014597-66vd2mdu	65	38	platform	platform	NN
cord-014597-66vd2mdu	65	39	approach	approach	NN
cord-014597-66vd2mdu	65	40	.	.	.
cord-014597-66vd2mdu	65	41	.	.	.
cord-014597-66vd2mdu	66	1	Matriptase	Matriptase	NNP
cord-014597-66vd2mdu	66	2	knock	knock	NN
cord-014597-66vd2mdu	66	3	-	-	HYPH
cord-014597-66vd2mdu	66	4	out	out	NN
cord-014597-66vd2mdu	66	5	in	in	IN
cord-014597-66vd2mdu	66	6	CHO	CHO	NNP
cord-014597-66vd2mdu	66	7	cells	cell	NNS
cord-014597-66vd2mdu	66	8	prevents	prevent	VBZ
cord-014597-66vd2mdu	66	9	clipping	clip	VBG
cord-014597-66vd2mdu	66	10	of	of	IN
cord-014597-66vd2mdu	66	11	recombinant	recombinant	JJ
cord-014597-66vd2mdu	66	12	proteins	protein	NNS
cord-014597-66vd2mdu	66	13	.	.	.
cord-014597-66vd2mdu	67	1	a	a	DT
cord-014597-66vd2mdu	67	2	Serine	Serine	NNP
cord-014597-66vd2mdu	67	3	protease	protease	NN
cord-014597-66vd2mdu	67	4	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	67	5	protect	protect	VBP
cord-014597-66vd2mdu	67	6	model	model	NN
cord-014597-66vd2mdu	67	7	mAb	mab	NN
cord-014597-66vd2mdu	67	8	from	from	IN
cord-014597-66vd2mdu	67	9	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	67	10	degradation	degradation	NN
cord-014597-66vd2mdu	67	11	in	in	IN
cord-014597-66vd2mdu	67	12	CHO	CHO	NNP
cord-014597-66vd2mdu	67	13	-	-	HYPH
cord-014597-66vd2mdu	67	14	K1	K1	NNP
cord-014597-66vd2mdu	67	15	cell	cell	NN
cord-014597-66vd2mdu	67	16	derived	derive	VBN
cord-014597-66vd2mdu	67	17	conditioned	condition	VBN
cord-014597-66vd2mdu	67	18	medium	medium	NN
cord-014597-66vd2mdu	67	19	.	.	.
cord-014597-66vd2mdu	68	1	The	the	DT
cord-014597-66vd2mdu	68	2	model	model	NN
cord-014597-66vd2mdu	68	3	mAb	mab	NN
cord-014597-66vd2mdu	68	4	was	be	VBD
cord-014597-66vd2mdu	68	5	incubated	incubate	VBN
cord-014597-66vd2mdu	68	6	in	in	IN
cord-014597-66vd2mdu	68	7	conditioned	condition	VBN
cord-014597-66vd2mdu	68	8	medium	medium	NN
cord-014597-66vd2mdu	68	9	for	for	IN
cord-014597-66vd2mdu	68	10	0h	0h	CD
cord-014597-66vd2mdu	68	11	or	or	CC
cord-014597-66vd2mdu	68	12	48h	48h	NNS
cord-014597-66vd2mdu	68	13	at	at	IN
cord-014597-66vd2mdu	68	14	37	37	CD
cord-014597-66vd2mdu	68	15	°	°	NN
cord-014597-66vd2mdu	68	16	C	c	NN
cord-014597-66vd2mdu	68	17	,	,	,
cord-014597-66vd2mdu	68	18	subsequently	subsequently	RB
cord-014597-66vd2mdu	68	19	samples	sample	NNS
cord-014597-66vd2mdu	68	20	were	be	VBD
cord-014597-66vd2mdu	68	21	analyzed	analyze	VBN
cord-014597-66vd2mdu	68	22	by	by	IN
cord-014597-66vd2mdu	68	23	western	western	JJ
cord-014597-66vd2mdu	68	24	blot	blot	NN
cord-014597-66vd2mdu	68	25	.	.	.
cord-014597-66vd2mdu	69	1	Broad	broad	JJ
cord-014597-66vd2mdu	69	2	spectrum	spectrum	NN
cord-014597-66vd2mdu	69	3	serine	serine	NN
cord-014597-66vd2mdu	69	4	protease	protease	NN
cord-014597-66vd2mdu	69	5	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	69	6	(	(	-LRB-
cord-014597-66vd2mdu	69	7	Aprotinin	Aprotinin	NNP
cord-014597-66vd2mdu	69	8	,	,	,
cord-014597-66vd2mdu	69	9	Leupetin	Leupetin	NNP
cord-014597-66vd2mdu	69	10	)	)	-RRB-
cord-014597-66vd2mdu	69	11	were	be	VBD
cord-014597-66vd2mdu	69	12	added	add	VBN
cord-014597-66vd2mdu	69	13	during	during	IN
cord-014597-66vd2mdu	69	14	incubation	incubation	NN
cord-014597-66vd2mdu	69	15	.	.	.
cord-014597-66vd2mdu	70	1	Aprotinin	Aprotinin	NNP
cord-014597-66vd2mdu	70	2	and	and	CC
cord-014597-66vd2mdu	70	3	Leupetin	Leupetin	NNP
cord-014597-66vd2mdu	70	4	are	be	VBP
cord-014597-66vd2mdu	70	5	inhibiting	inhibit	VBG
cord-014597-66vd2mdu	70	6	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	70	7	degradation	degradation	NN
cord-014597-66vd2mdu	70	8	.	.	.
cord-014597-66vd2mdu	71	1	The	the	DT
cord-014597-66vd2mdu	71	2	intact	intact	JJ
cord-014597-66vd2mdu	71	3	mAb	mab	NN
cord-014597-66vd2mdu	71	4	(	(	-LRB-
cord-014597-66vd2mdu	71	5	upper	upper	JJ
cord-014597-66vd2mdu	71	6	band	band	NN
cord-014597-66vd2mdu	71	7	)	)	-RRB-
cord-014597-66vd2mdu	71	8	and	and	CC
cord-014597-66vd2mdu	71	9	the	the	DT
cord-014597-66vd2mdu	71	10	clipped	clip	VBN
cord-014597-66vd2mdu	71	11	mAb	mAb	NNS
cord-014597-66vd2mdu	71	12	(	(	-LRB-
cord-014597-66vd2mdu	71	13	lower	low	JJR
cord-014597-66vd2mdu	71	14	band	band	NN
cord-014597-66vd2mdu	71	15	)	)	-RRB-
cord-014597-66vd2mdu	71	16	are	be	VBP
cord-014597-66vd2mdu	71	17	indicated	indicate	VBN
cord-014597-66vd2mdu	71	18	by	by	IN
cord-014597-66vd2mdu	71	19	arrows	arrow	NNS
cord-014597-66vd2mdu	71	20	.	.	.
cord-014597-66vd2mdu	72	1	b	b	LS
cord-014597-66vd2mdu	72	2	Gene	gene	NN
cord-014597-66vd2mdu	72	3	expression	expression	NN
cord-014597-66vd2mdu	72	4	profiling	profiling	NN
cord-014597-66vd2mdu	72	5	of	of	IN
cord-014597-66vd2mdu	72	6	CHO	CHO	NNP
cord-014597-66vd2mdu	72	7	-	-	HYPH
cord-014597-66vd2mdu	72	8	K1	K1	NNP
cord-014597-66vd2mdu	72	9	versus	versus	IN
cord-014597-66vd2mdu	72	10	CHO	CHO	NNP
cord-014597-66vd2mdu	72	11	-	-	HYPH
cord-014597-66vd2mdu	72	12	A	A	NNP
cord-014597-66vd2mdu	72	13	by	by	IN
cord-014597-66vd2mdu	72	14	NGS	NGS	NNP
cord-014597-66vd2mdu	72	15	.	.	.
cord-014597-66vd2mdu	73	1	Shown	show	VBN
cord-014597-66vd2mdu	73	2	is	be	VBZ
cord-014597-66vd2mdu	73	3	the	the	DT
cord-014597-66vd2mdu	73	4	gene	gene	NN
cord-014597-66vd2mdu	73	5	expression	expression	NN
cord-014597-66vd2mdu	73	6	profile	profile	NN
cord-014597-66vd2mdu	73	7	of	of	IN
cord-014597-66vd2mdu	73	8	"	"	``
cord-014597-66vd2mdu	73	9	secreted	secrete	VBN
cord-014597-66vd2mdu	73	10	/	/	SYM
cord-014597-66vd2mdu	73	11	shedded	shedde	VBN
cord-014597-66vd2mdu	73	12	members	member	NNS
cord-014597-66vd2mdu	73	13	of	of	IN
cord-014597-66vd2mdu	73	14	the	the	DT
cord-014597-66vd2mdu	73	15	S1A	s1a	NN
cord-014597-66vd2mdu	73	16	trypsin	trypsin	NN
cord-014597-66vd2mdu	73	17	-	-	HYPH
cord-014597-66vd2mdu	73	18	like	like	JJ
cord-014597-66vd2mdu	73	19	serine	serine	NN
cord-014597-66vd2mdu	73	20	protease	protease	NN
cord-014597-66vd2mdu	73	21	family	family	NN
cord-014597-66vd2mdu	73	22	"	"	''
cord-014597-66vd2mdu	73	23	for	for	IN
cord-014597-66vd2mdu	73	24	CHO	CHO	NNP
cord-014597-66vd2mdu	73	25	-	-	HYPH
cord-014597-66vd2mdu	73	26	K1	K1	NNP
cord-014597-66vd2mdu	73	27	and	and	CC
cord-014597-66vd2mdu	73	28	CHO	CHO	NNP
cord-014597-66vd2mdu	73	29	-	-	HYPH
cord-014597-66vd2mdu	73	30	A	a	DT
cord-014597-66vd2mdu	73	31	cell	cell	NN
cord-014597-66vd2mdu	73	32	lines	line	NNS
cord-014597-66vd2mdu	73	33	using	use	VBG
cord-014597-66vd2mdu	73	34	next	next	JJ
cord-014597-66vd2mdu	73	35	generation	generation	NN
cord-014597-66vd2mdu	73	36	sequencing	sequencing	NN
cord-014597-66vd2mdu	73	37	.	.	.
cord-014597-66vd2mdu	74	1	The	the	DT
cord-014597-66vd2mdu	74	2	gene	gene	NN
cord-014597-66vd2mdu	74	3	expression	expression	NN
cord-014597-66vd2mdu	74	4	analysis	analysis	NN
cord-014597-66vd2mdu	74	5	highlights	highlight	NNS
cord-014597-66vd2mdu	74	6	that	that	IN
cord-014597-66vd2mdu	74	7	five	five	CD
cord-014597-66vd2mdu	74	8	proteases	protease	NNS
cord-014597-66vd2mdu	74	9	were	be	VBD
cord-014597-66vd2mdu	74	10	more	more	JJR
cord-014597-66vd2mdu	74	11	than	than	IN
cord-014597-66vd2mdu	74	12	1.5	1.5	CD
cord-014597-66vd2mdu	74	13	fold	fold	VB
cord-014597-66vd2mdu	74	14	higher	higher	RBR
cord-014597-66vd2mdu	74	15	expressed	express	VBN
cord-014597-66vd2mdu	74	16	in	in	IN
cord-014597-66vd2mdu	74	17	CHO	CHO	NNP
cord-014597-66vd2mdu	74	18	-	-	HYPH
cord-014597-66vd2mdu	74	19	K1	K1	NNP
cord-014597-66vd2mdu	74	20	cells	cell	NNS
cord-014597-66vd2mdu	74	21	(	(	-LRB-
cord-014597-66vd2mdu	74	22	labelled	label	VBN
cord-014597-66vd2mdu	74	23	with	with	IN
cord-014597-66vd2mdu	74	24	a	a	DT
cord-014597-66vd2mdu	74	25	red	red	JJ
cord-014597-66vd2mdu	74	26	asterix	asterix	NN
cord-014597-66vd2mdu	74	27	)	)	-RRB-
cord-014597-66vd2mdu	74	28	.	.	.
cord-014597-66vd2mdu	75	1	The	the	DT
cord-014597-66vd2mdu	75	2	y	y	NN
cord-014597-66vd2mdu	75	3	-	-	HYPH
cord-014597-66vd2mdu	75	4	axis	axis	NN
cord-014597-66vd2mdu	75	5	shows	show	VBZ
cord-014597-66vd2mdu	75	6	the	the	DT
cord-014597-66vd2mdu	75	7	transcript	transcript	NN
cord-014597-66vd2mdu	75	8	abundance	abundance	NN
cord-014597-66vd2mdu	75	9	as	as	IN
cord-014597-66vd2mdu	75	10	RPKM	RPKM	NNP
cord-014597-66vd2mdu	75	11	(	(	-LRB-
cord-014597-66vd2mdu	75	12	Reads	read	VBZ
cord-014597-66vd2mdu	75	13	Per	per	IN
cord-014597-66vd2mdu	75	14	Kilobase	Kilobase	NNP
cord-014597-66vd2mdu	75	15	of	of	IN
cord-014597-66vd2mdu	75	16	exon	exon	NN
cord-014597-66vd2mdu	75	17	model	model	NN
cord-014597-66vd2mdu	75	18	per	per	IN
cord-014597-66vd2mdu	75	19	Million	million	CD
cord-014597-66vd2mdu	75	20	mapped	map	VBN
cord-014597-66vd2mdu	75	21	reads	read	VBZ
cord-014597-66vd2mdu	75	22	)	)	-RRB-
cord-014597-66vd2mdu	75	23	.	.	.
cord-014597-66vd2mdu	76	1	c	c	NNP
cord-014597-66vd2mdu	76	2	siRNA	siRNA	NNP
cord-014597-66vd2mdu	76	3	knock	knock	VB
cord-014597-66vd2mdu	76	4	-	-	HYPH
cord-014597-66vd2mdu	76	5	down	down	NN
cord-014597-66vd2mdu	76	6	identifies	identify	VBZ
cord-014597-66vd2mdu	76	7	matriptase	matriptase	NN
cord-014597-66vd2mdu	76	8	as	as	IN
cord-014597-66vd2mdu	76	9	major	major	JJ
cord-014597-66vd2mdu	76	10	clipping	clip	VBG
cord-014597-66vd2mdu	76	11	protease	protease	NN
cord-014597-66vd2mdu	76	12	and	and	CC
cord-014597-66vd2mdu	76	13	CHO	CHO	NNP
cord-014597-66vd2mdu	76	14	matriptase	matriptase	NN
cord-014597-66vd2mdu	76	15	KO	KO	NNP
cord-014597-66vd2mdu	76	16	clone	clone	NN
cord-014597-66vd2mdu	76	17	shows	show	VBZ
cord-014597-66vd2mdu	76	18	no	no	DT
cord-014597-66vd2mdu	76	19	detectable	detectable	JJ
cord-014597-66vd2mdu	76	20	clipping	clip	VBG
cord-014597-66vd2mdu	76	21	activity	activity	NN
cord-014597-66vd2mdu	76	22	.	.	.
cord-014597-66vd2mdu	77	1	Upper	upper	JJ
cord-014597-66vd2mdu	77	2	figure	figure	NN
cord-014597-66vd2mdu	77	3	:	:	:
cord-014597-66vd2mdu	77	4	siRNAs	siRNAs	NNP
cord-014597-66vd2mdu	77	5	directed	direct	VBN
cord-014597-66vd2mdu	77	6	against	against	IN
cord-014597-66vd2mdu	77	7	the	the	DT
cord-014597-66vd2mdu	77	8	five	five	CD
cord-014597-66vd2mdu	77	9	protease	protease	NN
cord-014597-66vd2mdu	77	10	genes	gene	NNS
cord-014597-66vd2mdu	77	11	and	and	CC
cord-014597-66vd2mdu	77	12	scrambled	scramble	VBN
cord-014597-66vd2mdu	77	13	(	(	-LRB-
cord-014597-66vd2mdu	77	14	scr	scr	NNP
cord-014597-66vd2mdu	77	15	.	.	.
cord-014597-66vd2mdu	77	16	)	)	-RRB-
cord-014597-66vd2mdu	78	1	siRNA	siRNA	NNP
cord-014597-66vd2mdu	78	2	were	be	VBD
cord-014597-66vd2mdu	78	3	transfected	transfecte	VBN
cord-014597-66vd2mdu	78	4	and	and	CC
cord-014597-66vd2mdu	78	5	conditioned	condition	VBN
cord-014597-66vd2mdu	78	6	medium	medium	NN
cord-014597-66vd2mdu	78	7	was	be	VBD
cord-014597-66vd2mdu	78	8	collected	collect	VBN
cord-014597-66vd2mdu	78	9	three	three	CD
cord-014597-66vd2mdu	78	10	days	day	NNS
cord-014597-66vd2mdu	78	11	after	after	IN
cord-014597-66vd2mdu	78	12	transfection	transfection	NN
cord-014597-66vd2mdu	78	13	.	.	.
cord-014597-66vd2mdu	79	1	The	the	DT
cord-014597-66vd2mdu	79	2	model	model	NN
cord-014597-66vd2mdu	79	3	mAb	mab	NN
cord-014597-66vd2mdu	79	4	was	be	VBD
cord-014597-66vd2mdu	79	5	incubated	incubate	VBN
cord-014597-66vd2mdu	79	6	in	in	IN
cord-014597-66vd2mdu	79	7	fresh	fresh	JJ
cord-014597-66vd2mdu	79	8	medium	medium	NN
cord-014597-66vd2mdu	79	9	as	as	IN
cord-014597-66vd2mdu	79	10	control	control	NN
cord-014597-66vd2mdu	79	11	(	(	-LRB-
cord-014597-66vd2mdu	79	12	first	first	JJ
cord-014597-66vd2mdu	79	13	lane	lane	NN
cord-014597-66vd2mdu	79	14	)	)	-RRB-
cord-014597-66vd2mdu	79	15	and	and	CC
cord-014597-66vd2mdu	79	16	conditioned	condition	VBN
cord-014597-66vd2mdu	79	17	medium	medium	NN
cord-014597-66vd2mdu	79	18	from	from	IN
cord-014597-66vd2mdu	79	19	the	the	DT
cord-014597-66vd2mdu	79	20	siRNA	siRNA	NNP
cord-014597-66vd2mdu	79	21	transfected	transfecte	VBN
cord-014597-66vd2mdu	79	22	cells	cell	NNS
cord-014597-66vd2mdu	79	23	.	.	.
cord-014597-66vd2mdu	80	1	Samples	sample	NNS
cord-014597-66vd2mdu	80	2	were	be	VBD
cord-014597-66vd2mdu	80	3	analyzed	analyze	VBN
cord-014597-66vd2mdu	80	4	by	by	IN
cord-014597-66vd2mdu	80	5	western	western	JJ
cord-014597-66vd2mdu	80	6	blot	blot	NN
cord-014597-66vd2mdu	80	7	.	.	.
cord-014597-66vd2mdu	81	1	Only	only	RB
cord-014597-66vd2mdu	81	2	siRNA	siRNA	NNP
cord-014597-66vd2mdu	81	3	targeting	target	VBG
cord-014597-66vd2mdu	81	4	matriptase	matriptase	NNP
cord-014597-66vd2mdu	81	5	(	(	-LRB-
cord-014597-66vd2mdu	81	6	ST14	ST14	NNP
cord-014597-66vd2mdu	81	7	)	)	-RRB-
cord-014597-66vd2mdu	81	8	showed	show	VBD
cord-014597-66vd2mdu	81	9	reduced	reduce	VBN
cord-014597-66vd2mdu	81	10	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	81	11	degradation	degradation	NN
cord-014597-66vd2mdu	81	12	.	.	.
cord-014597-66vd2mdu	82	1	The	the	DT
cord-014597-66vd2mdu	82	2	intact	intact	JJ
cord-014597-66vd2mdu	82	3	mAb	mab	NN
cord-014597-66vd2mdu	82	4	(	(	-LRB-
cord-014597-66vd2mdu	82	5	upper	upper	JJ
cord-014597-66vd2mdu	82	6	band	band	NN
cord-014597-66vd2mdu	82	7	)	)	-RRB-
cord-014597-66vd2mdu	82	8	and	and	CC
cord-014597-66vd2mdu	82	9	the	the	DT
cord-014597-66vd2mdu	82	10	clipped	clip	VBN
cord-014597-66vd2mdu	82	11	mAb	mAb	NNS
cord-014597-66vd2mdu	82	12	(	(	-LRB-
cord-014597-66vd2mdu	82	13	lower	low	JJR
cord-014597-66vd2mdu	82	14	band	band	NN
cord-014597-66vd2mdu	82	15	)	)	-RRB-
cord-014597-66vd2mdu	82	16	are	be	VBP
cord-014597-66vd2mdu	82	17	indicated	indicate	VBN
cord-014597-66vd2mdu	82	18	by	by	IN
cord-014597-66vd2mdu	82	19	arrows	arrow	NNS
cord-014597-66vd2mdu	82	20	.	.	.
cord-014597-66vd2mdu	83	1	Lower	low	JJR
cord-014597-66vd2mdu	83	2	figure	figure	NN
cord-014597-66vd2mdu	84	1	:	:	:
cord-014597-66vd2mdu	84	2	The	the	DT
cord-014597-66vd2mdu	84	3	model	model	NN
cord-014597-66vd2mdu	84	4	mAb	mab	NN
cord-014597-66vd2mdu	84	5	was	be	VBD
cord-014597-66vd2mdu	84	6	incubated	incubate	VBN
cord-014597-66vd2mdu	84	7	for	for	IN
cord-014597-66vd2mdu	84	8	48h	48h	NNS
cord-014597-66vd2mdu	84	9	in	in	IN
cord-014597-66vd2mdu	84	10	conditioned	condition	VBN
cord-014597-66vd2mdu	84	11	medium	medium	NN
cord-014597-66vd2mdu	84	12	collected	collect	VBN
cord-014597-66vd2mdu	84	13	from	from	IN
cord-014597-66vd2mdu	84	14	wt	wt	NNP
cord-014597-66vd2mdu	84	15	CHO	CHO	NNP
cord-014597-66vd2mdu	84	16	-	-	HYPH
cord-014597-66vd2mdu	84	17	K1	K1	NNP
cord-014597-66vd2mdu	84	18	as	as	RB
cord-014597-66vd2mdu	84	19	well	well	RB
cord-014597-66vd2mdu	84	20	as	as	IN
cord-014597-66vd2mdu	84	21	the	the	DT
cord-014597-66vd2mdu	84	22	matriptase	matriptase	JJ
cord-014597-66vd2mdu	84	23	knockout	knockout	NN
cord-014597-66vd2mdu	84	24	clone	clone	NN
cord-014597-66vd2mdu	84	25	.	.	.
cord-014597-66vd2mdu	85	1	Samples	sample	NNS
cord-014597-66vd2mdu	85	2	were	be	VBD
cord-014597-66vd2mdu	85	3	analyzed	analyze	VBN
cord-014597-66vd2mdu	85	4	by	by	IN
cord-014597-66vd2mdu	85	5	western	western	JJ
cord-014597-66vd2mdu	85	6	blot	blot	NN
cord-014597-66vd2mdu	85	7	.	.	.
cord-014597-66vd2mdu	86	1	The	the	DT
cord-014597-66vd2mdu	86	2	intact	intact	JJ
cord-014597-66vd2mdu	86	3	mAb	mab	NN
cord-014597-66vd2mdu	86	4	(	(	-LRB-
cord-014597-66vd2mdu	86	5	upper	upper	JJ
cord-014597-66vd2mdu	86	6	band	band	NN
cord-014597-66vd2mdu	86	7	)	)	-RRB-
cord-014597-66vd2mdu	86	8	and	and	CC
cord-014597-66vd2mdu	86	9	the	the	DT
cord-014597-66vd2mdu	86	10	clipped	clip	VBN
cord-014597-66vd2mdu	86	11	mAb	mAb	NNS
cord-014597-66vd2mdu	86	12	(	(	-LRB-
cord-014597-66vd2mdu	86	13	lower	low	JJR
cord-014597-66vd2mdu	86	14	band	band	NN
cord-014597-66vd2mdu	86	15	)	)	-RRB-
cord-014597-66vd2mdu	86	16	are	be	VBP
cord-014597-66vd2mdu	86	17	indicated	indicate	VBN
cord-014597-66vd2mdu	86	18	by	by	IN
cord-014597-66vd2mdu	86	19	arrows	arrow	NNS
cord-014597-66vd2mdu	86	20	.	.	.
cord-014597-66vd2mdu	87	1	No	no	DT
cord-014597-66vd2mdu	87	2	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	87	3	degradation	degradation	NN
cord-014597-66vd2mdu	87	4	could	could	MD
cord-014597-66vd2mdu	87	5	be	be	VB
cord-014597-66vd2mdu	87	6	detected	detect	VBN
cord-014597-66vd2mdu	87	7	in	in	IN
cord-014597-66vd2mdu	87	8	the	the	DT
cord-014597-66vd2mdu	87	9	samples	sample	NNS
cord-014597-66vd2mdu	87	10	originating	originate	VBG
cord-014597-66vd2mdu	87	11	from	from	IN
cord-014597-66vd2mdu	87	12	the	the	DT
cord-014597-66vd2mdu	87	13	matriptase	matriptase	JJ
cord-014597-66vd2mdu	87	14	KO	KO	NNP
cord-014597-66vd2mdu	87	15	clone	clone	NN
cord-014597-66vd2mdu	87	16	.	.	.
cord-014597-66vd2mdu	88	1	d	d	NNP
cord-014597-66vd2mdu	88	2	Cell	Cell	NNP
cord-014597-66vd2mdu	88	3	growth	growth	NN
cord-014597-66vd2mdu	88	4	,	,	,
cord-014597-66vd2mdu	88	5	viability	viability	NN
cord-014597-66vd2mdu	88	6	and	and	CC
cord-014597-66vd2mdu	88	7	productivity	productivity	NN
cord-014597-66vd2mdu	88	8	in	in	IN
cord-014597-66vd2mdu	88	9	AMBR	AMBR	NNP
cord-014597-66vd2mdu	88	10	(	(	-LRB-
cord-014597-66vd2mdu	88	11	fed	fed	NNP
cord-014597-66vd2mdu	88	12	batch	batch	NN
cord-014597-66vd2mdu	88	13	with	with	IN
cord-014597-66vd2mdu	88	14	temperature	temperature	NN
cord-014597-66vd2mdu	88	15	shift	shift	NN
cord-014597-66vd2mdu	88	16	)	)	-RRB-
cord-014597-66vd2mdu	88	17	.	.	.
cord-014597-66vd2mdu	89	1	Cell	cell	NN
cord-014597-66vd2mdu	89	2	growth	growth	NN
cord-014597-66vd2mdu	89	3	,	,	,
cord-014597-66vd2mdu	89	4	viability	viability	NN
cord-014597-66vd2mdu	89	5	and	and	CC
cord-014597-66vd2mdu	89	6	volumetric	volumetric	JJ
cord-014597-66vd2mdu	89	7	productivity	productivity	NN
cord-014597-66vd2mdu	89	8	profiles	profile	NNS
cord-014597-66vd2mdu	89	9	of	of	IN
cord-014597-66vd2mdu	89	10	wt	wt	NNP
cord-014597-66vd2mdu	89	11	CHO	CHO	NNP
cord-014597-66vd2mdu	89	12	-	-	HYPH
cord-014597-66vd2mdu	89	13	K1	K1	NNP
cord-014597-66vd2mdu	89	14	(	(	-LRB-
cord-014597-66vd2mdu	89	15	red	red	JJ
cord-014597-66vd2mdu	89	16	circles	circle	NNS
cord-014597-66vd2mdu	89	17	,	,	,
cord-014597-66vd2mdu	89	18	N=2	N=2	NNP
cord-014597-66vd2mdu	89	19	)	)	-RRB-
cord-014597-66vd2mdu	89	20	and	and	CC
cord-014597-66vd2mdu	89	21	matriptase	matriptase	NNP
cord-014597-66vd2mdu	89	22	KO	KO	NNP
cord-014597-66vd2mdu	89	23	clone	clone	NN
cord-014597-66vd2mdu	89	24	(	(	-LRB-
cord-014597-66vd2mdu	89	25	blue	blue	JJ
cord-014597-66vd2mdu	89	26	squares	square	NNS
cord-014597-66vd2mdu	89	27	,	,	,
cord-014597-66vd2mdu	89	28	N=1	N=1	NNP
cord-014597-66vd2mdu	89	29	)	)	-RRB-
cord-014597-66vd2mdu	89	30	cultivated	cultivate	VBD
cord-014597-66vd2mdu	89	31	in	in	IN
cord-014597-66vd2mdu	89	32	15-mL	15-ml	CD
cord-014597-66vd2mdu	89	33	AMBR	AMBR	NNP
cord-014597-66vd2mdu	89	34	.	.	.
cord-014597-66vd2mdu	90	1	No	no	DT
cord-014597-66vd2mdu	90	2	significant	significant	JJ
cord-014597-66vd2mdu	90	3	differences	difference	NNS
cord-014597-66vd2mdu	90	4	were	be	VBD
cord-014597-66vd2mdu	90	5	seen	see	VBN
cord-014597-66vd2mdu	90	6	between	between	IN
cord-014597-66vd2mdu	90	7	WT	WT	NNP
cord-014597-66vd2mdu	90	8	and	and	CC
cord-014597-66vd2mdu	90	9	matriptase	matriptase	NN
cord-014597-66vd2mdu	90	10	KO	KO	NNP
cord-014597-66vd2mdu	90	11	clone	clone	NN
cord-014597-66vd2mdu	90	12	regarding	regard	VBG
cord-014597-66vd2mdu	90	13	cell	cell	NN
cord-014597-66vd2mdu	90	14	growth	growth	NN
cord-014597-66vd2mdu	90	15	and	and	CC
cord-014597-66vd2mdu	90	16	viability	viability	NN
cord-014597-66vd2mdu	90	17	.	.	.
cord-014597-66vd2mdu	91	1	Comparable	comparable	JJ
cord-014597-66vd2mdu	91	2	or	or	CC
cord-014597-66vd2mdu	91	3	slightly	slightly	RB
cord-014597-66vd2mdu	91	4	higher	high	JJR
cord-014597-66vd2mdu	91	5	productivity	productivity	NN
cord-014597-66vd2mdu	91	6	was	be	VBD
cord-014597-66vd2mdu	91	7	detected	detect	VBN
cord-014597-66vd2mdu	91	8	for	for	IN
cord-014597-66vd2mdu	91	9	the	the	DT
cord-014597-66vd2mdu	91	10	matriptase	matriptase	JJ
cord-014597-66vd2mdu	91	11	KO	KO	NNP
cord-014597-66vd2mdu	91	12	clone	clone	NN
cord-014597-66vd2mdu	91	13	compared	compare	VBN
cord-014597-66vd2mdu	91	14	to	to	IN
cord-014597-66vd2mdu	91	15	the	the	DT
cord-014597-66vd2mdu	91	16	WT	WT	NNP
cord-014597-66vd2mdu	91	17	.	.	.
cord-014597-66vd2mdu	92	1	e	e	LS
cord-014597-66vd2mdu	92	2	Significant	significant	JJ
cord-014597-66vd2mdu	92	3	reduced	reduce	VBN
cord-014597-66vd2mdu	92	4	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	92	5	clipping	clip	VBG
cord-014597-66vd2mdu	92	6	applying	apply	VBG
cord-014597-66vd2mdu	92	7	matriptase	matriptase	NNP
cord-014597-66vd2mdu	92	8	KO	KO	NNP
cord-014597-66vd2mdu	92	9	clone	clone	NN
cord-014597-66vd2mdu	92	10	.	.	.
cord-014597-66vd2mdu	93	1	The	the	DT
cord-014597-66vd2mdu	93	2	model	model	NN
cord-014597-66vd2mdu	93	3	mAb	mab	NN
cord-014597-66vd2mdu	93	4	was	be	VBD
cord-014597-66vd2mdu	93	5	stable	stable	JJ
cord-014597-66vd2mdu	93	6	expressed	express	VBN
cord-014597-66vd2mdu	93	7	in	in	IN
cord-014597-66vd2mdu	93	8	CHO	CHO	NNP
cord-014597-66vd2mdu	93	9	-	-	HYPH
cord-014597-66vd2mdu	93	10	K1	K1	NNP
cord-014597-66vd2mdu	93	11	(	(	-LRB-
cord-014597-66vd2mdu	93	12	WT	WT	NNP
cord-014597-66vd2mdu	93	13	)	)	-RRB-
cord-014597-66vd2mdu	93	14	as	as	RB
cord-014597-66vd2mdu	93	15	well	well	RB
cord-014597-66vd2mdu	93	16	as	as	IN
cord-014597-66vd2mdu	93	17	the	the	DT
cord-014597-66vd2mdu	93	18	CHO	CHO	NNP
cord-014597-66vd2mdu	93	19	-	-	HYPH
cord-014597-66vd2mdu	93	20	K1	K1	NNP
cord-014597-66vd2mdu	93	21	matriptase	matriptase	NN
cord-014597-66vd2mdu	93	22	KO	KO	NNP
cord-014597-66vd2mdu	93	23	clone	clone	NN
cord-014597-66vd2mdu	93	24	.	.	.
cord-014597-66vd2mdu	94	1	Samples	sample	NNS
cord-014597-66vd2mdu	94	2	were	be	VBD
cord-014597-66vd2mdu	94	3	analyzed	analyze	VBN
cord-014597-66vd2mdu	94	4	by	by	IN
cord-014597-66vd2mdu	94	5	western	western	JJ
cord-014597-66vd2mdu	94	6	blot	blot	NN
cord-014597-66vd2mdu	94	7	.	.	.
cord-014597-66vd2mdu	95	1	The	the	DT
cord-014597-66vd2mdu	95	2	intact	intact	JJ
cord-014597-66vd2mdu	95	3	mAb	mab	NN
cord-014597-66vd2mdu	95	4	(	(	-LRB-
cord-014597-66vd2mdu	95	5	upper	upper	JJ
cord-014597-66vd2mdu	95	6	band	band	NN
cord-014597-66vd2mdu	95	7	)	)	-RRB-
cord-014597-66vd2mdu	95	8	and	and	CC
cord-014597-66vd2mdu	95	9	the	the	DT
cord-014597-66vd2mdu	95	10	clipped	clip	VBN
cord-014597-66vd2mdu	95	11	mAb	mAb	NNS
cord-014597-66vd2mdu	95	12	(	(	-LRB-
cord-014597-66vd2mdu	95	13	lower	low	JJR
cord-014597-66vd2mdu	95	14	band	band	NN
cord-014597-66vd2mdu	95	15	)	)	-RRB-
cord-014597-66vd2mdu	95	16	are	be	VBP
cord-014597-66vd2mdu	95	17	indicated	indicate	VBN
cord-014597-66vd2mdu	95	18	by	by	IN
cord-014597-66vd2mdu	95	19	arrows	arrow	NNS
cord-014597-66vd2mdu	95	20	.	.	.
cord-014597-66vd2mdu	96	1	Significant	significant	JJ
cord-014597-66vd2mdu	96	2	reduced	reduce	VBN
cord-014597-66vd2mdu	96	3	proteolytic	proteolytic	JJ
cord-014597-66vd2mdu	96	4	degradation	degradation	NN
cord-014597-66vd2mdu	96	5	could	could	MD
cord-014597-66vd2mdu	96	6	be	be	VB
cord-014597-66vd2mdu	96	7	detected	detect	VBN
cord-014597-66vd2mdu	96	8	in	in	IN
cord-014597-66vd2mdu	96	9	the	the	DT
cord-014597-66vd2mdu	96	10	samples	sample	NNS
cord-014597-66vd2mdu	96	11	originating	originate	VBG
cord-014597-66vd2mdu	96	12	from	from	IN
cord-014597-66vd2mdu	96	13	the	the	DT
cord-014597-66vd2mdu	96	14	matriptase	matriptase	JJ
cord-014597-66vd2mdu	96	15	KO	KO	NNP
cord-014597-66vd2mdu	96	16	clone	clone	NN
cord-014597-66vd2mdu	96	17	(	(	-LRB-
cord-014597-66vd2mdu	96	18	3	3	CD
cord-014597-66vd2mdu	96	19	samples	sample	NNS
cord-014597-66vd2mdu	96	20	each	each	DT
cord-014597-66vd2mdu	96	21	is	be	VBZ
cord-014597-66vd2mdu	96	22	shown	show	VBN
cord-014597-66vd2mdu	96	23	for	for	IN
cord-014597-66vd2mdu	96	24	wt	wt	NN
cord-014597-66vd2mdu	96	25	and	and	CC
cord-014597-66vd2mdu	96	26	KO	KO	NNP
cord-014597-66vd2mdu	96	27	cells	cell	NNS
cord-014597-66vd2mdu	96	28	)	)	-RRB-
cord-014597-66vd2mdu	97	1	The	the	DT
cord-014597-66vd2mdu	97	2	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	97	3	of	of	IN
cord-014597-66vd2mdu	97	4	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	97	5	proteins	protein	NNS
cord-014597-66vd2mdu	97	6	is	be	VBZ
cord-014597-66vd2mdu	97	7	a	a	DT
cord-014597-66vd2mdu	97	8	critical	critical	JJ
cord-014597-66vd2mdu	97	9	quality	quality	NN
cord-014597-66vd2mdu	97	10	attribute	attribute	NN
cord-014597-66vd2mdu	97	11	(	(	-LRB-
cord-014597-66vd2mdu	97	12	CQA	CQA	NNP
cord-014597-66vd2mdu	97	13	)	)	-RRB-
cord-014597-66vd2mdu	97	14	and	and	CC
cord-014597-66vd2mdu	97	15	needs	need	VBZ
cord-014597-66vd2mdu	97	16	to	to	TO
cord-014597-66vd2mdu	97	17	be	be	VB
cord-014597-66vd2mdu	97	18	analyzed	analyze	VBN
cord-014597-66vd2mdu	97	19	during	during	IN
cord-014597-66vd2mdu	97	20	cell	cell	NN
cord-014597-66vd2mdu	97	21	line	line	NN
cord-014597-66vd2mdu	97	22	and	and	CC
cord-014597-66vd2mdu	97	23	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	97	24	development	development	NN
cord-014597-66vd2mdu	97	25	.	.	.
cord-014597-66vd2mdu	98	1	The	the	DT
cord-014597-66vd2mdu	98	2	current	current	JJ
cord-014597-66vd2mdu	98	3	methods	method	NNS
cord-014597-66vd2mdu	98	4	for	for	IN
cord-014597-66vd2mdu	98	5	analyzing	analyze	VBG
cord-014597-66vd2mdu	98	6	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	98	7	are	be	VBP
cord-014597-66vd2mdu	98	8	mainly	mainly	RB
cord-014597-66vd2mdu	98	9	based	base	VBN
cord-014597-66vd2mdu	98	10	on	on	IN
cord-014597-66vd2mdu	98	11	the	the	DT
cord-014597-66vd2mdu	98	12	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	98	13	release	release	NN
cord-014597-66vd2mdu	98	14	of	of	IN
cord-014597-66vd2mdu	98	15	glycans	glycan	NNS
cord-014597-66vd2mdu	98	16	.	.	.
cord-014597-66vd2mdu	99	1	They	-PRON-	PRP
cord-014597-66vd2mdu	99	2	are	be	VBP
cord-014597-66vd2mdu	99	3	tedious	tedious	JJ
cord-014597-66vd2mdu	99	4	and	and	CC
cord-014597-66vd2mdu	99	5	offer	offer	VBP
cord-014597-66vd2mdu	99	6	only	only	RB
cord-014597-66vd2mdu	99	7	limited	limited	JJ
cord-014597-66vd2mdu	99	8	throughput	throughput	NN
cord-014597-66vd2mdu	99	9	,	,	,
cord-014597-66vd2mdu	99	10	which	which	WDT
cord-014597-66vd2mdu	99	11	makes	make	VBZ
cord-014597-66vd2mdu	99	12	them	-PRON-	PRP
cord-014597-66vd2mdu	99	13	unsuitable	unsuitable	JJ
cord-014597-66vd2mdu	99	14	for	for	IN
cord-014597-66vd2mdu	99	15	cell	cell	NN
cord-014597-66vd2mdu	99	16	line	line	NN
cord-014597-66vd2mdu	99	17	development	development	NN
cord-014597-66vd2mdu	99	18	work	work	NN
cord-014597-66vd2mdu	99	19	.	.	.
cord-014597-66vd2mdu	100	1	In	in	IN
cord-014597-66vd2mdu	100	2	this	this	DT
cord-014597-66vd2mdu	100	3	study	study	NN
cord-014597-66vd2mdu	100	4	we	-PRON-	PRP
cord-014597-66vd2mdu	100	5	evaluated	evaluate	VBD
cord-014597-66vd2mdu	100	6	a	a	DT
cord-014597-66vd2mdu	100	7	novel	novel	JJ
cord-014597-66vd2mdu	100	8	PAIA	PAIA	NNP
cord-014597-66vd2mdu	100	9	assay	assay	NN
cord-014597-66vd2mdu	100	10	for	for	IN
cord-014597-66vd2mdu	100	11	measuring	measure	VBG
cord-014597-66vd2mdu	100	12	intact	intact	JJ
cord-014597-66vd2mdu	100	13	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	100	14	with	with	IN
cord-014597-66vd2mdu	100	15	capture	capture	NN
cord-014597-66vd2mdu	100	16	beads	bead	NNS
cord-014597-66vd2mdu	100	17	and	and	CC
cord-014597-66vd2mdu	100	18	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	100	19	labeled	label	VBN
cord-014597-66vd2mdu	100	20	plant	plant	NN
cord-014597-66vd2mdu	100	21	lectins	lectin	NNS
cord-014597-66vd2mdu	100	22	to	to	TO
cord-014597-66vd2mdu	100	23	analyze	analyze	VB
cord-014597-66vd2mdu	100	24	glycans	glycan	NNS
cord-014597-66vd2mdu	100	25	in	in	IN
cord-014597-66vd2mdu	100	26	a	a	DT
cord-014597-66vd2mdu	100	27	high	high	JJ
cord-014597-66vd2mdu	100	28	throughput	throughput	NN
cord-014597-66vd2mdu	100	29	384-well	384-well	CD
cord-014597-66vd2mdu	100	30	plate	plate	NN
cord-014597-66vd2mdu	100	31	format	format	NN
cord-014597-66vd2mdu	100	32	.	.	.
cord-014597-66vd2mdu	101	1	Material	material	NN
cord-014597-66vd2mdu	101	2	and	and	CC
cord-014597-66vd2mdu	101	3	methods	method	NNS
cord-014597-66vd2mdu	101	4	Analytes	Analytes	NNPS
cord-014597-66vd2mdu	101	5	:	:	:
cord-014597-66vd2mdu	101	6	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	101	7	©	©	NNP
cord-014597-66vd2mdu	101	8	,	,	,
cord-014597-66vd2mdu	101	9	Mabthera	Mabthera	NNP
cord-014597-66vd2mdu	101	10	©	©	NNP
cord-014597-66vd2mdu	101	11	,	,	,
cord-014597-66vd2mdu	101	12	Arzerra	Arzerra	NNP
cord-014597-66vd2mdu	101	13	©	©	NNP
cord-014597-66vd2mdu	102	1	and	and	CC
cord-014597-66vd2mdu	102	2	Avastin	Avastin	NNP
cord-014597-66vd2mdu	102	3	©	©	NNP
cord-014597-66vd2mdu	102	4	.	.	.
cord-014597-66vd2mdu	103	1	Two	two	CD
cord-014597-66vd2mdu	103	2	glycoengineered	glycoengineered	JJ
cord-014597-66vd2mdu	103	3	variants	variant	NNS
cord-014597-66vd2mdu	103	4	of	of	IN
cord-014597-66vd2mdu	103	5	one	one	CD
cord-014597-66vd2mdu	103	6	IgG	igg	NN
cord-014597-66vd2mdu	103	7	were	be	VBD
cord-014597-66vd2mdu	103	8	kindly	kindly	RB
cord-014597-66vd2mdu	103	9	provided	provide	VBN
cord-014597-66vd2mdu	103	10	by	by	IN
cord-014597-66vd2mdu	103	11	Merck	Merck	NNP
cord-014597-66vd2mdu	103	12	(	(	-LRB-
cord-014597-66vd2mdu	103	13	Vevey	Vevey	NNP
cord-014597-66vd2mdu	103	14	,	,	,
cord-014597-66vd2mdu	103	15	Switzerland	Switzerland	NNP
cord-014597-66vd2mdu	103	16	)	)	-RRB-
cord-014597-66vd2mdu	103	17	.	.	.
cord-014597-66vd2mdu	104	1	All	all	DT
cord-014597-66vd2mdu	104	2	analytes	analyte	NNS
cord-014597-66vd2mdu	104	3	were	be	VBD
cord-014597-66vd2mdu	104	4	spiked	spike	VBN
cord-014597-66vd2mdu	104	5	into	into	IN
cord-014597-66vd2mdu	104	6	CHO	CHO	NNP
cord-014597-66vd2mdu	104	7	-	-	HYPH
cord-014597-66vd2mdu	104	8	K1	K1	NNP
cord-014597-66vd2mdu	104	9	cell	cell	NN
cord-014597-66vd2mdu	104	10	culture	culture	NN
cord-014597-66vd2mdu	104	11	supernatant	supernatant	JJ
cord-014597-66vd2mdu	104	12	or	or	CC
cord-014597-66vd2mdu	104	13	buffer	buffer	NN
cord-014597-66vd2mdu	104	14	,	,	,
cord-014597-66vd2mdu	104	15	diluted	dilute	VBN
cord-014597-66vd2mdu	104	16	1:1	1:1	CD
cord-014597-66vd2mdu	104	17	with	with	IN
cord-014597-66vd2mdu	104	18	a	a	DT
cord-014597-66vd2mdu	104	19	denaturation	denaturation	NN
cord-014597-66vd2mdu	104	20	solution	solution	NN
cord-014597-66vd2mdu	104	21	and	and	CC
cord-014597-66vd2mdu	104	22	incubated	incubate	VBN
cord-014597-66vd2mdu	104	23	at	at	IN
cord-014597-66vd2mdu	104	24	65	65	CD
cord-014597-66vd2mdu	104	25	or	or	CC
cord-014597-66vd2mdu	104	26	70	70	CD
cord-014597-66vd2mdu	104	27	°	°	NN
cord-014597-66vd2mdu	104	28	C	c	NN
cord-014597-66vd2mdu	104	29	for	for	IN
cord-014597-66vd2mdu	104	30	20	20	CD
cord-014597-66vd2mdu	104	31	minutes	minute	NNS
cord-014597-66vd2mdu	104	32	to	to	TO
cord-014597-66vd2mdu	104	33	expose	expose	VB
cord-014597-66vd2mdu	104	34	the	the	DT
cord-014597-66vd2mdu	104	35	Fc	Fc	NNP
cord-014597-66vd2mdu	104	36	glycans	glycan	NNS
cord-014597-66vd2mdu	104	37	.	.	.
cord-014597-66vd2mdu	105	1	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	105	2	samples	sample	NNS
cord-014597-66vd2mdu	105	3	were	be	VBD
cord-014597-66vd2mdu	105	4	analyzed	analyze	VBN
cord-014597-66vd2mdu	105	5	under	under	IN
cord-014597-66vd2mdu	105	6	denaturing	denaturing	JJ
cord-014597-66vd2mdu	105	7	conditions	condition	NNS
cord-014597-66vd2mdu	105	8	to	to	TO
cord-014597-66vd2mdu	105	9	detect	detect	VB
cord-014597-66vd2mdu	105	10	Fab	Fab	NNP
cord-014597-66vd2mdu	105	11	-	-	:
cord-014597-66vd2mdu	105	12	and	and	CC
cord-014597-66vd2mdu	105	13	Fc	Fc	NNP
cord-014597-66vd2mdu	105	14	-	-	HYPH
cord-014597-66vd2mdu	105	15	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	105	16	and	and	CC
cord-014597-66vd2mdu	105	17	in	in	IN
cord-014597-66vd2mdu	105	18	native	native	JJ
cord-014597-66vd2mdu	105	19	conditions	condition	NNS
cord-014597-66vd2mdu	105	20	for	for	IN
cord-014597-66vd2mdu	105	21	Fab	fab	JJ
cord-014597-66vd2mdu	105	22	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	105	23	only	only	RB
cord-014597-66vd2mdu	105	24	.	.	.
cord-014597-66vd2mdu	106	1	10μL	10μl	CD
cord-014597-66vd2mdu	106	2	of	of	IN
cord-014597-66vd2mdu	106	3	pretreated	pretreate	VBN
cord-014597-66vd2mdu	106	4	sample	sample	NN
cord-014597-66vd2mdu	106	5	was	be	VBD
cord-014597-66vd2mdu	106	6	added	add	VBN
cord-014597-66vd2mdu	106	7	to	to	IN
cord-014597-66vd2mdu	106	8	each	each	DT
cord-014597-66vd2mdu	106	9	well	well	NN
cord-014597-66vd2mdu	106	10	of	of	IN
cord-014597-66vd2mdu	106	11	the	the	DT
cord-014597-66vd2mdu	106	12	special	special	JJ
cord-014597-66vd2mdu	106	13	384-well	384-well	CD
cord-014597-66vd2mdu	106	14	PAIAplate	paiaplate	NN
cord-014597-66vd2mdu	106	15	,	,	,
cord-014597-66vd2mdu	106	16	containing	contain	VBG
cord-014597-66vd2mdu	106	17	labeled	label	VBN
cord-014597-66vd2mdu	106	18	lectin	lectin	NN
cord-014597-66vd2mdu	106	19	and	and	CC
cord-014597-66vd2mdu	106	20	capture	capture	NN
cord-014597-66vd2mdu	106	21	beads	bead	NNS
cord-014597-66vd2mdu	106	22	.	.	.
cord-014597-66vd2mdu	107	1	The	the	DT
cord-014597-66vd2mdu	107	2	microplate	microplate	NN
cord-014597-66vd2mdu	107	3	was	be	VBD
cord-014597-66vd2mdu	107	4	incubated	incubate	VBN
cord-014597-66vd2mdu	107	5	for	for	IN
cord-014597-66vd2mdu	107	6	45	45	CD
cord-014597-66vd2mdu	107	7	minutes	minute	NNS
cord-014597-66vd2mdu	107	8	at	at	IN
cord-014597-66vd2mdu	107	9	1800	1800	CD
cord-014597-66vd2mdu	107	10	rpm	rpm	NN
cord-014597-66vd2mdu	107	11	on	on	IN
cord-014597-66vd2mdu	107	12	an	an	DT
cord-014597-66vd2mdu	107	13	orbital	orbital	JJ
cord-014597-66vd2mdu	107	14	shaker	shaker	NN
cord-014597-66vd2mdu	107	15	at	at	IN
cord-014597-66vd2mdu	107	16	room	room	NN
cord-014597-66vd2mdu	107	17	temperature	temperature	NN
cord-014597-66vd2mdu	107	18	and	and	CC
cord-014597-66vd2mdu	107	19	spun	spin	VBD
cord-014597-66vd2mdu	107	20	down	down	RP
cord-014597-66vd2mdu	107	21	at	at	IN
cord-014597-66vd2mdu	107	22	500	500	CD
cord-014597-66vd2mdu	107	23	xg	xg	NNS
cord-014597-66vd2mdu	107	24	.	.	.
cord-014597-66vd2mdu	108	1	The	the	DT
cord-014597-66vd2mdu	108	2	read	read	NN
cord-014597-66vd2mdu	108	3	-	-	HYPH
cord-014597-66vd2mdu	108	4	out	out	NN
cord-014597-66vd2mdu	108	5	was	be	VBD
cord-014597-66vd2mdu	108	6	done	do	VBN
cord-014597-66vd2mdu	108	7	on	on	IN
cord-014597-66vd2mdu	108	8	a	a	DT
cord-014597-66vd2mdu	108	9	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	108	10	microscope	microscope	NN
cord-014597-66vd2mdu	108	11	(	(	-LRB-
cord-014597-66vd2mdu	108	12	SynenTec	SynenTec	NNP
cord-014597-66vd2mdu	108	13	,	,	,
cord-014597-66vd2mdu	108	14	Elmshorn	Elmshorn	NNP
cord-014597-66vd2mdu	108	15	,	,	,
cord-014597-66vd2mdu	108	16	Germany	Germany	NNP
cord-014597-66vd2mdu	108	17	)	)	-RRB-
cord-014597-66vd2mdu	108	18	in	in	IN
cord-014597-66vd2mdu	108	19	less	less	JJR
cord-014597-66vd2mdu	108	20	than	than	IN
cord-014597-66vd2mdu	108	21	five	five	CD
cord-014597-66vd2mdu	108	22	minutes	minute	NNS
cord-014597-66vd2mdu	108	23	.	.	.
cord-014597-66vd2mdu	109	1	Results	result	NNS
cord-014597-66vd2mdu	109	2	Figure	Figure	NNP
cord-014597-66vd2mdu	109	3	1a	1a	CD
cord-014597-66vd2mdu	109	4	:	:	:
cord-014597-66vd2mdu	110	1	Lectin	Lectin	NNP
cord-014597-66vd2mdu	110	2	binding	bind	VBG
cord-014597-66vd2mdu	110	3	profiles	profile	NNS
cord-014597-66vd2mdu	110	4	of	of	IN
cord-014597-66vd2mdu	110	5	different	different	JJ
cord-014597-66vd2mdu	110	6	IgGs	IgGs	NNP
cord-014597-66vd2mdu	110	7	.	.	.
cord-014597-66vd2mdu	111	1	The	the	DT
cord-014597-66vd2mdu	111	2	analysis	analysis	NN
cord-014597-66vd2mdu	111	3	of	of	IN
cord-014597-66vd2mdu	111	4	different	different	JJ
cord-014597-66vd2mdu	111	5	IgG	igg	NN
cord-014597-66vd2mdu	111	6	results	result	NNS
cord-014597-66vd2mdu	111	7	in	in	IN
cord-014597-66vd2mdu	111	8	lectin	lectin	NN
cord-014597-66vd2mdu	111	9	binding	bind	VBG
cord-014597-66vd2mdu	111	10	profiles	profile	NNS
cord-014597-66vd2mdu	111	11	which	which	WDT
cord-014597-66vd2mdu	111	12	show	show	VBP
cord-014597-66vd2mdu	111	13	the	the	DT
cord-014597-66vd2mdu	111	14	different	different	JJ
cord-014597-66vd2mdu	111	15	degrees	degree	NNS
cord-014597-66vd2mdu	111	16	in	in	IN
cord-014597-66vd2mdu	111	17	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	111	18	.	.	.
cord-014597-66vd2mdu	112	1	High	high	JJ
cord-014597-66vd2mdu	112	2	abundance	abundance	NN
cord-014597-66vd2mdu	112	3	of	of	IN
cord-014597-66vd2mdu	112	4	sugars	sugar	NNS
cord-014597-66vd2mdu	112	5	leads	lead	VBZ
cord-014597-66vd2mdu	112	6	to	to	IN
cord-014597-66vd2mdu	112	7	high	high	JJ
cord-014597-66vd2mdu	112	8	binding	binding	JJ
cord-014597-66vd2mdu	112	9	rates	rate	NNS
cord-014597-66vd2mdu	112	10	of	of	IN
cord-014597-66vd2mdu	112	11	the	the	DT
cord-014597-66vd2mdu	112	12	lectin	lectin	NN
cord-014597-66vd2mdu	112	13	for	for	IN
cord-014597-66vd2mdu	112	14	the	the	DT
cord-014597-66vd2mdu	112	15	respective	respective	JJ
cord-014597-66vd2mdu	112	16	sugar	sugar	NN
cord-014597-66vd2mdu	112	17	.	.	.
cord-014597-66vd2mdu	113	1	Avastin	Avastin	NNP
cord-014597-66vd2mdu	113	2	has	have	VBZ
cord-014597-66vd2mdu	113	3	a	a	DT
cord-014597-66vd2mdu	113	4	very	very	RB
cord-014597-66vd2mdu	113	5	low	low	JJ
cord-014597-66vd2mdu	113	6	degree	degree	NN
cord-014597-66vd2mdu	113	7	of	of	IN
cord-014597-66vd2mdu	113	8	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	113	9	and	and	CC
cord-014597-66vd2mdu	113	10	high	high	JJ
cord-014597-66vd2mdu	113	11	mannose	mannose	NN
cord-014597-66vd2mdu	113	12	species	specie	NNS
cord-014597-66vd2mdu	113	13	compared	compare	VBN
cord-014597-66vd2mdu	113	14	to	to	IN
cord-014597-66vd2mdu	113	15	Mabthera	Mabthera	NNP
cord-014597-66vd2mdu	113	16	and	and	CC
cord-014597-66vd2mdu	113	17	Arzerra	Arzerra	NNP
cord-014597-66vd2mdu	113	18	(	(	-LRB-
cord-014597-66vd2mdu	113	19	Fig	Fig	NNP
cord-014597-66vd2mdu	113	20	.	.	.
cord-014597-66vd2mdu	113	21	1a	1a	CD
cord-014597-66vd2mdu	113	22	)	)	-RRB-
cord-014597-66vd2mdu	113	23	.	.	.
cord-014597-66vd2mdu	114	1	Only	only	RB
cord-014597-66vd2mdu	114	2	Arzerra	Arzerra	NNP
cord-014597-66vd2mdu	114	3	is	be	VBZ
cord-014597-66vd2mdu	114	4	carrying	carry	VBG
cord-014597-66vd2mdu	114	5	glycans	glycan	NNS
cord-014597-66vd2mdu	114	6	with	with	IN
cord-014597-66vd2mdu	114	7	2	2	CD
cord-014597-66vd2mdu	114	8	-	-	SYM
cord-014597-66vd2mdu	114	9	6	6	CD
cord-014597-66vd2mdu	114	10	linked	link	VBN
cord-014597-66vd2mdu	114	11	sialic	sialic	NN
cord-014597-66vd2mdu	114	12	acids	acid	NNS
cord-014597-66vd2mdu	114	13	.	.	.
cord-014597-66vd2mdu	115	1	These	these	DT
cord-014597-66vd2mdu	115	2	findings	finding	NNS
cord-014597-66vd2mdu	115	3	are	be	VBP
cord-014597-66vd2mdu	115	4	in	in	IN
cord-014597-66vd2mdu	115	5	line	line	NN
cord-014597-66vd2mdu	115	6	with	with	IN
cord-014597-66vd2mdu	115	7	results	result	NNS
cord-014597-66vd2mdu	115	8	from	from	IN
cord-014597-66vd2mdu	115	9	literature	literature	NN
cord-014597-66vd2mdu	115	10	[	[	-LRB-
cord-014597-66vd2mdu	115	11	1	1	CD
cord-014597-66vd2mdu	115	12	]	]	-RRB-
cord-014597-66vd2mdu	115	13	.	.	.
cord-014597-66vd2mdu	116	1	Figure	figure	NN
cord-014597-66vd2mdu	116	2	1b	1b	CD
cord-014597-66vd2mdu	116	3	:	:	:
cord-014597-66vd2mdu	116	4	Distinction	distinction	NN
cord-014597-66vd2mdu	116	5	between	between	IN
cord-014597-66vd2mdu	116	6	Fc	Fc	NNP
cord-014597-66vd2mdu	116	7	and	and	CC
cord-014597-66vd2mdu	116	8	Fab	fab	JJ
cord-014597-66vd2mdu	116	9	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	116	10	in	in	IN
cord-014597-66vd2mdu	116	11	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	116	12	.	.	.
cord-014597-66vd2mdu	117	1	Without	without	IN
cord-014597-66vd2mdu	117	2	denaturation	denaturation	NN
cord-014597-66vd2mdu	117	3	only	only	RB
cord-014597-66vd2mdu	117	4	the	the	DT
cord-014597-66vd2mdu	117	5	Fab	fab	JJ
cord-014597-66vd2mdu	117	6	glycans	glycan	NNS
cord-014597-66vd2mdu	117	7	are	be	VBP
cord-014597-66vd2mdu	117	8	detectable	detectable	JJ
cord-014597-66vd2mdu	117	9	in	in	IN
cord-014597-66vd2mdu	117	10	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	117	11	.	.	.
cord-014597-66vd2mdu	118	1	Denaturation	Denaturation	NNP
cord-014597-66vd2mdu	118	2	leads	lead	VBZ
cord-014597-66vd2mdu	118	3	to	to	IN
cord-014597-66vd2mdu	118	4	additional	additional	JJ
cord-014597-66vd2mdu	118	5	exposure	exposure	NN
cord-014597-66vd2mdu	118	6	of	of	IN
cord-014597-66vd2mdu	118	7	the	the	DT
cord-014597-66vd2mdu	118	8	Fc	Fc	NNP
cord-014597-66vd2mdu	118	9	glycans	glycan	NNS
cord-014597-66vd2mdu	118	10	and	and	CC
cord-014597-66vd2mdu	118	11	thus	thus	RB
cord-014597-66vd2mdu	118	12	higher	high	JJR
cord-014597-66vd2mdu	118	13	lectin	lectin	NN
cord-014597-66vd2mdu	118	14	binding	bind	VBG
cord-014597-66vd2mdu	118	15	rates	rate	NNS
cord-014597-66vd2mdu	118	16	compared	compare	VBN
cord-014597-66vd2mdu	118	17	to	to	IN
cord-014597-66vd2mdu	118	18	native	native	JJ
cord-014597-66vd2mdu	118	19	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	118	20	.	.	.
cord-014597-66vd2mdu	119	1	GNA	GNA	NNP
cord-014597-66vd2mdu	119	2	and	and	CC
cord-014597-66vd2mdu	119	3	NPL	NPL	NNP
cord-014597-66vd2mdu	119	4	only	only	RB
cord-014597-66vd2mdu	119	5	bind	bind	VBP
cord-014597-66vd2mdu	119	6	to	to	IN
cord-014597-66vd2mdu	119	7	denatured	denatured	JJ
cord-014597-66vd2mdu	119	8	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	119	9	indicating	indicate	VBG
cord-014597-66vd2mdu	119	10	that	that	IN
cord-014597-66vd2mdu	119	11	the	the	DT
cord-014597-66vd2mdu	119	12	high	high	JJ
cord-014597-66vd2mdu	119	13	mannose	mannose	NN
cord-014597-66vd2mdu	119	14	glycans	glycan	NNS
cord-014597-66vd2mdu	119	15	are	be	VBP
cord-014597-66vd2mdu	119	16	only	only	RB
cord-014597-66vd2mdu	119	17	present	present	JJ
cord-014597-66vd2mdu	119	18	on	on	IN
cord-014597-66vd2mdu	119	19	the	the	DT
cord-014597-66vd2mdu	119	20	Fc	Fc	NNP
cord-014597-66vd2mdu	119	21	part	part	NN
cord-014597-66vd2mdu	119	22	.	.	.
cord-014597-66vd2mdu	120	1	The	the	DT
cord-014597-66vd2mdu	120	2	equal	equal	JJ
cord-014597-66vd2mdu	120	3	SNA	SNA	NNP
cord-014597-66vd2mdu	120	4	binding	binding	NN
cord-014597-66vd2mdu	120	5	rates	rate	NNS
cord-014597-66vd2mdu	120	6	for	for	IN
cord-014597-66vd2mdu	120	7	both	both	DT
cord-014597-66vd2mdu	120	8	conditions	condition	NNS
cord-014597-66vd2mdu	120	9	confirm	confirm	VBP
cord-014597-66vd2mdu	120	10	that	that	IN
cord-014597-66vd2mdu	120	11	the	the	DT
cord-014597-66vd2mdu	120	12	2	2	CD
cord-014597-66vd2mdu	120	13	-	-	SYM
cord-014597-66vd2mdu	120	14	6	6	CD
cord-014597-66vd2mdu	120	15	linked	link	VBN
cord-014597-66vd2mdu	120	16	sialic	sialic	JJ
cord-014597-66vd2mdu	120	17	acids	acid	NNS
cord-014597-66vd2mdu	120	18	are	be	VBP
cord-014597-66vd2mdu	120	19	almost	almost	RB
cord-014597-66vd2mdu	120	20	exclusively	exclusively	RB
cord-014597-66vd2mdu	120	21	found	find	VBN
cord-014597-66vd2mdu	120	22	on	on	IN
cord-014597-66vd2mdu	120	23	the	the	DT
cord-014597-66vd2mdu	120	24	Fab	fab	JJ
cord-014597-66vd2mdu	120	25	part	part	NN
cord-014597-66vd2mdu	120	26	.	.	.
cord-014597-66vd2mdu	121	1	This	this	DT
cord-014597-66vd2mdu	121	2	is	be	VBZ
cord-014597-66vd2mdu	121	3	in	in	IN
cord-014597-66vd2mdu	121	4	agreement	agreement	NN
cord-014597-66vd2mdu	121	5	with	with	IN
cord-014597-66vd2mdu	121	6	published	publish	VBN
cord-014597-66vd2mdu	121	7	data	datum	NNS
cord-014597-66vd2mdu	121	8	[	[	-LRB-
cord-014597-66vd2mdu	121	9	2	2	CD
cord-014597-66vd2mdu	121	10	]	]	-RRB-
cord-014597-66vd2mdu	121	11	.	.	.
cord-014597-66vd2mdu	122	1	Figure	figure	NN
cord-014597-66vd2mdu	122	2	1c	1c	CD
cord-014597-66vd2mdu	122	3	:	:	:
cord-014597-66vd2mdu	123	1	Lectin	Lectin	NNP
cord-014597-66vd2mdu	123	2	binding	bind	VBG
cord-014597-66vd2mdu	123	3	rates	rate	NNS
cord-014597-66vd2mdu	123	4	correlate	correlate	VBP
cord-014597-66vd2mdu	123	5	with	with	IN
cord-014597-66vd2mdu	123	6	the	the	DT
cord-014597-66vd2mdu	123	7	levels	level	NNS
cord-014597-66vd2mdu	123	8	of	of	IN
cord-014597-66vd2mdu	123	9	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	123	10	and	and	CC
cord-014597-66vd2mdu	123	11	fucosylation	fucosylation	NN
cord-014597-66vd2mdu	123	12	.	.	.
cord-014597-66vd2mdu	124	1	Increasing	increase	VBG
cord-014597-66vd2mdu	124	2	degrees	degree	NNS
cord-014597-66vd2mdu	124	3	of	of	IN
cord-014597-66vd2mdu	124	4	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	124	5	in	in	IN
cord-014597-66vd2mdu	124	6	the	the	DT
cord-014597-66vd2mdu	124	7	mixtures	mixture	NNS
cord-014597-66vd2mdu	124	8	of	of	IN
cord-014597-66vd2mdu	124	9	the	the	DT
cord-014597-66vd2mdu	124	10	glycan	glycan	NN
cord-014597-66vd2mdu	124	11	variants	variant	NNS
cord-014597-66vd2mdu	124	12	from	from	IN
cord-014597-66vd2mdu	124	13	Merck	Merck	NNP
cord-014597-66vd2mdu	124	14	lead	lead	VBP
cord-014597-66vd2mdu	124	15	to	to	IN
cord-014597-66vd2mdu	124	16	higher	high	JJR
cord-014597-66vd2mdu	124	17	lectin	lectin	NN
cord-014597-66vd2mdu	124	18	binding	bind	VBG
cord-014597-66vd2mdu	124	19	rates	rate	NNS
cord-014597-66vd2mdu	124	20	for	for	IN
cord-014597-66vd2mdu	124	21	all	all	DT
cord-014597-66vd2mdu	124	22	galactose	galactose	NN
cord-014597-66vd2mdu	124	23	and	and	CC
cord-014597-66vd2mdu	124	24	fucose	fucose	JJ
cord-014597-66vd2mdu	124	25	markers	marker	NNS
cord-014597-66vd2mdu	124	26	proving	prove	VBG
cord-014597-66vd2mdu	124	27	that	that	IN
cord-014597-66vd2mdu	124	28	quantitative	quantitative	JJ
cord-014597-66vd2mdu	124	29	analysis	analysis	NN
cord-014597-66vd2mdu	124	30	can	can	MD
cord-014597-66vd2mdu	124	31	be	be	VB
cord-014597-66vd2mdu	124	32	performed	perform	VBN
cord-014597-66vd2mdu	124	33	with	with	IN
cord-014597-66vd2mdu	124	34	these	these	DT
cord-014597-66vd2mdu	124	35	assays	assay	NNS
cord-014597-66vd2mdu	124	36	.	.	.
cord-014597-66vd2mdu	125	1	The	the	DT
cord-014597-66vd2mdu	125	2	ConA	ConA	NNP
cord-014597-66vd2mdu	125	3	lectin	lectin	NN
cord-014597-66vd2mdu	125	4	which	which	WDT
cord-014597-66vd2mdu	125	5	binds	bind	VBZ
cord-014597-66vd2mdu	125	6	to	to	IN
cord-014597-66vd2mdu	125	7	the	the	DT
cord-014597-66vd2mdu	125	8	common	common	JJ
cord-014597-66vd2mdu	125	9	core	core	NN
cord-014597-66vd2mdu	125	10	mannose	mannose	NN
cord-014597-66vd2mdu	125	11	glycan	glycan	NN
cord-014597-66vd2mdu	125	12	motive	motive	NN
cord-014597-66vd2mdu	125	13	remains	remain	VBZ
cord-014597-66vd2mdu	125	14	at	at	IN
cord-014597-66vd2mdu	125	15	the	the	DT
cord-014597-66vd2mdu	125	16	same	same	JJ
cord-014597-66vd2mdu	125	17	level	level	NN
cord-014597-66vd2mdu	125	18	,	,	,
cord-014597-66vd2mdu	125	19	suggesting	suggest	VBG
cord-014597-66vd2mdu	125	20	that	that	IN
cord-014597-66vd2mdu	125	21	the	the	DT
cord-014597-66vd2mdu	125	22	Fc	Fc	NNP
cord-014597-66vd2mdu	125	23	glycans	glycan	NNS
cord-014597-66vd2mdu	125	24	were	be	VBD
cord-014597-66vd2mdu	125	25	similarly	similarly	RB
cord-014597-66vd2mdu	125	26	exposed	expose	VBN
cord-014597-66vd2mdu	125	27	in	in	IN
cord-014597-66vd2mdu	125	28	all	all	DT
cord-014597-66vd2mdu	125	29	samples	sample	NNS
cord-014597-66vd2mdu	125	30	.	.	.
cord-014597-66vd2mdu	126	1	The	the	DT
cord-014597-66vd2mdu	126	2	results	result	NNS
cord-014597-66vd2mdu	126	3	demonstrate	demonstrate	VBP
cord-014597-66vd2mdu	126	4	that	that	IN
cord-014597-66vd2mdu	126	5	PAIA	PAIA	NNP
cord-014597-66vd2mdu	126	6	assays	assay	NNS
cord-014597-66vd2mdu	126	7	are	be	VBP
cord-014597-66vd2mdu	126	8	capable	capable	JJ
cord-014597-66vd2mdu	126	9	of	of	IN
cord-014597-66vd2mdu	126	10	quickly	quickly	RB
cord-014597-66vd2mdu	126	11	detecting	detect	VBG
cord-014597-66vd2mdu	126	12	differences	difference	NNS
cord-014597-66vd2mdu	126	13	in	in	IN
cord-014597-66vd2mdu	126	14	glycan	glycan	NN
cord-014597-66vd2mdu	126	15	patterns	pattern	NNS
cord-014597-66vd2mdu	126	16	of	of	IN
cord-014597-66vd2mdu	126	17	different	different	JJ
cord-014597-66vd2mdu	126	18	antibodies	antibody	NNS
cord-014597-66vd2mdu	126	19	.	.	.
cord-014597-66vd2mdu	127	1	In	in	IN
cord-014597-66vd2mdu	127	2	addition	addition	NN
cord-014597-66vd2mdu	127	3	it	-PRON-	PRP
cord-014597-66vd2mdu	127	4	was	be	VBD
cord-014597-66vd2mdu	127	5	shown	show	VBN
cord-014597-66vd2mdu	127	6	that	that	IN
cord-014597-66vd2mdu	127	7	glycan	glycan	NN
cord-014597-66vd2mdu	127	8	variants	variant	NNS
cord-014597-66vd2mdu	127	9	of	of	IN
cord-014597-66vd2mdu	127	10	the	the	DT
cord-014597-66vd2mdu	127	11	same	same	JJ
cord-014597-66vd2mdu	127	12	IgG	igg	NN
cord-014597-66vd2mdu	127	13	can	can	MD
cord-014597-66vd2mdu	127	14	be	be	VB
cord-014597-66vd2mdu	127	15	analyzed	analyze	VBN
cord-014597-66vd2mdu	127	16	quantitatively	quantitatively	RB
cord-014597-66vd2mdu	127	17	.	.	.
cord-014597-66vd2mdu	128	1	And	and	CC
cord-014597-66vd2mdu	128	2	finally	finally	RB
cord-014597-66vd2mdu	128	3	we	-PRON-	PRP
cord-014597-66vd2mdu	128	4	could	could	MD
cord-014597-66vd2mdu	128	5	confirm	confirm	VB
cord-014597-66vd2mdu	128	6	the	the	DT
cord-014597-66vd2mdu	128	7	differences	difference	NNS
cord-014597-66vd2mdu	128	8	in	in	IN
cord-014597-66vd2mdu	128	9	Fab	Fab	NNP
cord-014597-66vd2mdu	128	10	and	and	CC
cord-014597-66vd2mdu	128	11	Fc	Fc	NNP
cord-014597-66vd2mdu	128	12	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	128	13	in	in	IN
cord-014597-66vd2mdu	128	14	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	128	15	.	.	.
cord-014597-66vd2mdu	129	1	We	-PRON-	PRP
cord-014597-66vd2mdu	129	2	believe	believe	VBP
cord-014597-66vd2mdu	129	3	that	that	IN
cord-014597-66vd2mdu	129	4	bead	bead	NN
cord-014597-66vd2mdu	129	5	-	-	HYPH
cord-014597-66vd2mdu	129	6	based	base	VBN
cord-014597-66vd2mdu	129	7	assays	assay	NNS
cord-014597-66vd2mdu	129	8	with	with	IN
cord-014597-66vd2mdu	129	9	lectins	lectin	NNS
cord-014597-66vd2mdu	129	10	have	have	VBP
cord-014597-66vd2mdu	129	11	a	a	DT
cord-014597-66vd2mdu	129	12	great	great	JJ
cord-014597-66vd2mdu	129	13	potential	potential	NN
cord-014597-66vd2mdu	129	14	for	for	IN
cord-014597-66vd2mdu	129	15	monitoring	monitor	VBG
cord-014597-66vd2mdu	129	16	product	product	NN
cord-014597-66vd2mdu	129	17	quality	quality	NN
cord-014597-66vd2mdu	129	18	early	early	RB
cord-014597-66vd2mdu	129	19	in	in	IN
cord-014597-66vd2mdu	129	20	the	the	DT
cord-014597-66vd2mdu	129	21	development	development	NN
cord-014597-66vd2mdu	129	22	process	process	NN
cord-014597-66vd2mdu	129	23	.	.	.
cord-014597-66vd2mdu	130	1	Background	Background	NNP
cord-014597-66vd2mdu	130	2	Gene	Gene	NNP
cord-014597-66vd2mdu	130	3	-	-	HYPH
cord-014597-66vd2mdu	130	4	and	and	CC
cord-014597-66vd2mdu	130	5	cell	cell	NN
cord-014597-66vd2mdu	130	6	therapy	therapy	NN
cord-014597-66vd2mdu	130	7	-	-	HYPH
cord-014597-66vd2mdu	130	8	based	base	VBN
cord-014597-66vd2mdu	130	9	medicines	medicine	NNS
cord-014597-66vd2mdu	130	10	are	be	VBP
cord-014597-66vd2mdu	130	11	experiencing	experience	VBG
cord-014597-66vd2mdu	130	12	resurgence	resurgence	NN
cord-014597-66vd2mdu	130	13	due	due	IN
cord-014597-66vd2mdu	130	14	to	to	IN
cord-014597-66vd2mdu	130	15	the	the	DT
cord-014597-66vd2mdu	130	16	introduction	introduction	NN
cord-014597-66vd2mdu	130	17	of	of	IN
cord-014597-66vd2mdu	130	18	"	"	``
cord-014597-66vd2mdu	130	19	next	next	JJ
cord-014597-66vd2mdu	130	20	generation	generation	NN
cord-014597-66vd2mdu	130	21	"	"	''
cord-014597-66vd2mdu	130	22	transfer	transfer	NN
cord-014597-66vd2mdu	130	23	viral	viral	JJ
cord-014597-66vd2mdu	130	24	vectors	vector	NNS
cord-014597-66vd2mdu	130	25	,	,	,
cord-014597-66vd2mdu	130	26	which	which	WDT
cord-014597-66vd2mdu	130	27	have	have	VBP
cord-014597-66vd2mdu	130	28	demonstrated	demonstrate	VBN
cord-014597-66vd2mdu	130	29	improved	improved	JJ
cord-014597-66vd2mdu	130	30	safety	safety	NN
cord-014597-66vd2mdu	130	31	and	and	CC
cord-014597-66vd2mdu	130	32	efficacy	efficacy	NN
cord-014597-66vd2mdu	130	33	.	.	.
cord-014597-66vd2mdu	131	1	Adeno	Adeno	NNP
cord-014597-66vd2mdu	131	2	associated	associate	VBD
cord-014597-66vd2mdu	131	3	viruses	virus	NNS
cord-014597-66vd2mdu	131	4	(	(	-LRB-
cord-014597-66vd2mdu	131	5	AAV	AAV	NNP
cord-014597-66vd2mdu	131	6	)	)	-RRB-
cord-014597-66vd2mdu	131	7	and	and	CC
cord-014597-66vd2mdu	132	1	lentiviruses	lentiviruse	NNS
cord-014597-66vd2mdu	132	2	are	be	VBP
cord-014597-66vd2mdu	132	3	very	very	RB
cord-014597-66vd2mdu	132	4	commonly	commonly	RB
cord-014597-66vd2mdu	132	5	used	use	VBN
cord-014597-66vd2mdu	132	6	in	in	IN
cord-014597-66vd2mdu	132	7	therapeutics	therapeutic	NNS
cord-014597-66vd2mdu	132	8	and	and	CC
cord-014597-66vd2mdu	132	9	often	often	RB
cord-014597-66vd2mdu	132	10	produced	produce	VBN
cord-014597-66vd2mdu	132	11	by	by	IN
cord-014597-66vd2mdu	132	12	transient	transient	JJ
cord-014597-66vd2mdu	132	13	gene	gene	NN
cord-014597-66vd2mdu	132	14	expression	expression	NN
cord-014597-66vd2mdu	132	15	,	,	,
cord-014597-66vd2mdu	132	16	using	use	VBG
cord-014597-66vd2mdu	132	17	PEI	PEI	NNP
cord-014597-66vd2mdu	132	18	-	-	HYPH
cord-014597-66vd2mdu	132	19	mediated	mediate	VBN
cord-014597-66vd2mdu	132	20	transient	transient	JJ
cord-014597-66vd2mdu	132	21	transfection	transfection	NN
cord-014597-66vd2mdu	132	22	in	in	IN
cord-014597-66vd2mdu	132	23	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	132	24	or	or	CC
cord-014597-66vd2mdu	132	25	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	132	26	T	t	NN
cord-014597-66vd2mdu	132	27	cells	cell	NNS
cord-014597-66vd2mdu	132	28	[	[	-LRB-
cord-014597-66vd2mdu	132	29	1	1	CD
cord-014597-66vd2mdu	132	30	]	]	-RRB-
cord-014597-66vd2mdu	132	31	.	.	.
cord-014597-66vd2mdu	133	1	The	the	DT
cord-014597-66vd2mdu	133	2	critical	critical	JJ
cord-014597-66vd2mdu	133	3	raw	raw	JJ
cord-014597-66vd2mdu	133	4	materials	material	NNS
cord-014597-66vd2mdu	133	5	needed	need	VBN
cord-014597-66vd2mdu	133	6	for	for	IN
cord-014597-66vd2mdu	133	7	cGMP	cGMP	NNP
cord-014597-66vd2mdu	133	8	vector	vector	NN
cord-014597-66vd2mdu	133	9	production	production	NN
cord-014597-66vd2mdu	133	10	must	must	MD
cord-014597-66vd2mdu	133	11	be	be	VB
cord-014597-66vd2mdu	133	12	sourced	source	VBN
cord-014597-66vd2mdu	133	13	from	from	IN
cord-014597-66vd2mdu	133	14	approved	approve	VBN
cord-014597-66vd2mdu	133	15	suppliers	supplier	NNS
cord-014597-66vd2mdu	133	16	and	and	CC
cord-014597-66vd2mdu	133	17	should	should	MD
cord-014597-66vd2mdu	133	18	have	have	VB
cord-014597-66vd2mdu	133	19	gone	go	VBN
cord-014597-66vd2mdu	133	20	through	through	IN
cord-014597-66vd2mdu	133	21	a	a	DT
cord-014597-66vd2mdu	133	22	rigorous	rigorous	JJ
cord-014597-66vd2mdu	133	23	testing	testing	NN
cord-014597-66vd2mdu	133	24	program	program	NN
cord-014597-66vd2mdu	133	25	to	to	TO
cord-014597-66vd2mdu	133	26	reduce	reduce	VB
cord-014597-66vd2mdu	133	27	the	the	DT
cord-014597-66vd2mdu	133	28	risk	risk	NN
cord-014597-66vd2mdu	133	29	of	of	IN
cord-014597-66vd2mdu	133	30	introducing	introduce	VBG
cord-014597-66vd2mdu	133	31	adventitious	adventitious	JJ
cord-014597-66vd2mdu	133	32	agents	agent	NNS
cord-014597-66vd2mdu	133	33	into	into	IN
cord-014597-66vd2mdu	133	34	the	the	DT
cord-014597-66vd2mdu	133	35	production	production	NN
cord-014597-66vd2mdu	133	36	process	process	NN
cord-014597-66vd2mdu	133	37	.	.	.
cord-014597-66vd2mdu	134	1	Correspondingly	correspondingly	RB
cord-014597-66vd2mdu	134	2	,	,	,
cord-014597-66vd2mdu	134	3	the	the	DT
cord-014597-66vd2mdu	134	4	PEI	PEI	NNP
cord-014597-66vd2mdu	134	5	transfection	transfection	NN
cord-014597-66vd2mdu	134	6	reagent	reagent	NN
cord-014597-66vd2mdu	134	7	must	must	MD
cord-014597-66vd2mdu	134	8	also	also	RB
cord-014597-66vd2mdu	134	9	be	be	VB
cord-014597-66vd2mdu	134	10	sourced	source	VBN
cord-014597-66vd2mdu	134	11	from	from	IN
cord-014597-66vd2mdu	134	12	a	a	DT
cord-014597-66vd2mdu	134	13	qualified	qualified	JJ
cord-014597-66vd2mdu	134	14	supplier	supplier	NN
cord-014597-66vd2mdu	134	15	,	,	,
cord-014597-66vd2mdu	134	16	and	and	CC
cord-014597-66vd2mdu	134	17	have	have	VBP
cord-014597-66vd2mdu	134	18	gone	go	VBN
cord-014597-66vd2mdu	134	19	through	through	IN
cord-014597-66vd2mdu	134	20	rigorous	rigorous	JJ
cord-014597-66vd2mdu	134	21	testing	testing	NN
cord-014597-66vd2mdu	134	22	to	to	TO
cord-014597-66vd2mdu	134	23	ensure	ensure	VB
cord-014597-66vd2mdu	134	24	reliable	reliable	JJ
cord-014597-66vd2mdu	134	25	transfection	transfection	NN
cord-014597-66vd2mdu	134	26	efficiencies	efficiency	NNS
cord-014597-66vd2mdu	134	27	,	,	,
cord-014597-66vd2mdu	134	28	and	and	CC
cord-014597-66vd2mdu	134	29	hence	hence	RB
cord-014597-66vd2mdu	134	30	reproducible	reproducible	JJ
cord-014597-66vd2mdu	134	31	virus	virus	NN
cord-014597-66vd2mdu	134	32	production	production	NN
cord-014597-66vd2mdu	134	33	yields	yield	NNS
cord-014597-66vd2mdu	134	34	.	.	.
cord-014597-66vd2mdu	135	1	Here	here	RB
cord-014597-66vd2mdu	135	2	,	,	,
cord-014597-66vd2mdu	135	3	we	-PRON-	PRP
cord-014597-66vd2mdu	135	4	present	present	VBP
cord-014597-66vd2mdu	136	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	136	2	®	®	NN
cord-014597-66vd2mdu	136	3	and	and	CC
cord-014597-66vd2mdu	137	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	137	2	®	®	NNPS
cord-014597-66vd2mdu	137	3	-HQ	-hq	NN
cord-014597-66vd2mdu	137	4	,	,	,
cord-014597-66vd2mdu	137	5	the	the	DT
cord-014597-66vd2mdu	137	6	unique	unique	JJ
cord-014597-66vd2mdu	137	7	PEI	PEI	NNP
cord-014597-66vd2mdu	137	8	-	-	HYPH
cord-014597-66vd2mdu	137	9	based	base	VBN
cord-014597-66vd2mdu	137	10	transfection	transfection	NN
cord-014597-66vd2mdu	137	11	reagents	reagent	NNS
cord-014597-66vd2mdu	137	12	suitable	suitable	JJ
cord-014597-66vd2mdu	137	13	for	for	IN
cord-014597-66vd2mdu	137	14	use	use	NN
cord-014597-66vd2mdu	137	15	in	in	IN
cord-014597-66vd2mdu	137	16	process	process	NN
cord-014597-66vd2mdu	137	17	development	development	NN
cord-014597-66vd2mdu	137	18	and	and	CC
cord-014597-66vd2mdu	137	19	in	in	IN
cord-014597-66vd2mdu	137	20	cGMP	cGMP	NNP
cord-014597-66vd2mdu	137	21	biomanufacturing	biomanufacturing	NN
cord-014597-66vd2mdu	137	22	,	,	,
cord-014597-66vd2mdu	137	23	respectively	respectively	RB
cord-014597-66vd2mdu	137	24	.	.	.
cord-014597-66vd2mdu	138	1	Unlike	unlike	IN
cord-014597-66vd2mdu	138	2	commercially	commercially	RB
cord-014597-66vd2mdu	138	3	available	available	JJ
cord-014597-66vd2mdu	138	4	PEIs	pei	NNS
cord-014597-66vd2mdu	138	5	,	,	,
cord-014597-66vd2mdu	138	6	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	139	1	®	®	VB
cord-014597-66vd2mdu	139	2	benefits	benefit	NNS
cord-014597-66vd2mdu	139	3	from	from	IN
cord-014597-66vd2mdu	139	4	extensive	extensive	JJ
cord-014597-66vd2mdu	139	5	research	research	NN
cord-014597-66vd2mdu	139	6	and	and	CC
cord-014597-66vd2mdu	139	7	development	development	NN
cord-014597-66vd2mdu	139	8	in	in	IN
cord-014597-66vd2mdu	139	9	polymer	polymer	NN
cord-014597-66vd2mdu	139	10	chemistry	chemistry	NN
cord-014597-66vd2mdu	139	11	and	and	CC
cord-014597-66vd2mdu	139	12	formulation	formulation	NN
cord-014597-66vd2mdu	139	13	for	for	IN
cord-014597-66vd2mdu	139	14	mammalian	mammalian	JJ
cord-014597-66vd2mdu	139	15	cell	cell	NN
cord-014597-66vd2mdu	139	16	transfection	transfection	NN
cord-014597-66vd2mdu	139	17	.	.	.
cord-014597-66vd2mdu	140	1	We	-PRON-	PRP
cord-014597-66vd2mdu	140	2	further	further	RB
cord-014597-66vd2mdu	140	3	demonstrate	demonstrate	VBP
cord-014597-66vd2mdu	140	4	that	that	IN
cord-014597-66vd2mdu	140	5	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	140	6	®	®	NN
cord-014597-66vd2mdu	140	7	and	and	CC
cord-014597-66vd2mdu	141	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	141	2	®	®	NNPS
cord-014597-66vd2mdu	141	3	-HQ	-hq	CC
cord-014597-66vd2mdu	141	4	are	be	VBP
cord-014597-66vd2mdu	141	5	the	the	DT
cord-014597-66vd2mdu	141	6	reagents	reagent	NNS
cord-014597-66vd2mdu	141	7	of	of	IN
cord-014597-66vd2mdu	141	8	choice	choice	NN
cord-014597-66vd2mdu	141	9	for	for	IN
cord-014597-66vd2mdu	141	10	virus	virus	NN
cord-014597-66vd2mdu	141	11	production	production	NN
cord-014597-66vd2mdu	141	12	runs	run	VBZ
cord-014597-66vd2mdu	141	13	in	in	IN
cord-014597-66vd2mdu	141	14	most	most	JJS
cord-014597-66vd2mdu	141	15	cell	cell	NN
cord-014597-66vd2mdu	141	16	culture	culture	NN
cord-014597-66vd2mdu	141	17	systems	system	NNS
cord-014597-66vd2mdu	141	18	,	,	,
cord-014597-66vd2mdu	141	19	hence	hence	RB
cord-014597-66vd2mdu	141	20	facilitating	facilitate	VBG
cord-014597-66vd2mdu	141	21	the	the	DT
cord-014597-66vd2mdu	141	22	transition	transition	NN
cord-014597-66vd2mdu	141	23	from	from	IN
cord-014597-66vd2mdu	141	24	initial	initial	JJ
cord-014597-66vd2mdu	141	25	optimization	optimization	NN
cord-014597-66vd2mdu	141	26	during	during	IN
cord-014597-66vd2mdu	141	27	process	process	NN
cord-014597-66vd2mdu	141	28	development	development	NN
cord-014597-66vd2mdu	141	29	up	up	IN
cord-014597-66vd2mdu	141	30	to	to	IN
cord-014597-66vd2mdu	141	31	large	large	JJ
cord-014597-66vd2mdu	141	32	-	-	HYPH
cord-014597-66vd2mdu	141	33	scale	scale	NN
cord-014597-66vd2mdu	141	34	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	141	35	viral	viral	JJ
cord-014597-66vd2mdu	141	36	vector	vector	NN
cord-014597-66vd2mdu	141	37	production	production	NN
cord-014597-66vd2mdu	141	38	in	in	IN
cord-014597-66vd2mdu	141	39	adherent	adherent	NN
cord-014597-66vd2mdu	141	40	or	or	CC
cord-014597-66vd2mdu	141	41	suspension	suspension	NN
cord-014597-66vd2mdu	141	42	cells	cell	NNS
cord-014597-66vd2mdu	141	43	.	.	.
cord-014597-66vd2mdu	142	1	Manufacturing	manufacturing	NN
cord-014597-66vd2mdu	142	2	process	process	NN
cord-014597-66vd2mdu	142	3	of	of	IN
cord-014597-66vd2mdu	143	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	143	2	®	®	NN
cord-014597-66vd2mdu	143	3	and	and	CC
cord-014597-66vd2mdu	144	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	144	2	®	®	NNPS
cord-014597-66vd2mdu	144	3	-HQ	-hq	NN
cord-014597-66vd2mdu	144	4	reagents	reagent	NNS
cord-014597-66vd2mdu	144	5	.	.	.
cord-014597-66vd2mdu	145	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	146	1	®	®	VB
cord-014597-66vd2mdu	146	2	and	and	CC
cord-014597-66vd2mdu	147	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	147	2	®	®	NNPS
cord-014597-66vd2mdu	147	3	-HQ	-hq	CC
cord-014597-66vd2mdu	147	4	are	be	VBP
cord-014597-66vd2mdu	147	5	fully	fully	RB
cord-014597-66vd2mdu	147	6	synthetic	synthetic	JJ
cord-014597-66vd2mdu	147	7	reagents	reagent	NNS
cord-014597-66vd2mdu	147	8	,	,	,
cord-014597-66vd2mdu	147	9	free	free	JJ
cord-014597-66vd2mdu	147	10	of	of	IN
cord-014597-66vd2mdu	147	11	any	any	DT
cord-014597-66vd2mdu	147	12	animal	animal	NN
cord-014597-66vd2mdu	147	13	-	-	HYPH
cord-014597-66vd2mdu	147	14	origin	origin	NN
cord-014597-66vd2mdu	147	15	components	component	NNS
cord-014597-66vd2mdu	147	16	.	.	.
cord-014597-66vd2mdu	148	1	In	in	IN
cord-014597-66vd2mdu	148	2	comparison	comparison	NN
cord-014597-66vd2mdu	148	3	to	to	IN
cord-014597-66vd2mdu	148	4	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	148	5	®	®	NNP
cord-014597-66vd2mdu	148	6	,	,	,
cord-014597-66vd2mdu	148	7	a	a	DT
cord-014597-66vd2mdu	148	8	more	more	RBR
cord-014597-66vd2mdu	148	9	extensive	extensive	JJ
cord-014597-66vd2mdu	148	10	number	number	NN
cord-014597-66vd2mdu	148	11	of	of	IN
cord-014597-66vd2mdu	148	12	Quality	Quality	NNP
cord-014597-66vd2mdu	148	13	Controls	Controls	NNPS
cord-014597-66vd2mdu	148	14	are	be	VBP
cord-014597-66vd2mdu	148	15	performed	perform	VBN
cord-014597-66vd2mdu	148	16	on	on	IN
cord-014597-66vd2mdu	148	17	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	148	18	®	®	NNPS
cord-014597-66vd2mdu	148	19	-HQ	-hq	NN
cord-014597-66vd2mdu	148	20	to	to	TO
cord-014597-66vd2mdu	148	21	enable	enable	VB
cord-014597-66vd2mdu	148	22	its	-PRON-	PRP$
cord-014597-66vd2mdu	148	23	use	use	NN
cord-014597-66vd2mdu	148	24	as	as	IN
cord-014597-66vd2mdu	148	25	a	a	DT
cord-014597-66vd2mdu	148	26	qualified	qualified	JJ
cord-014597-66vd2mdu	148	27	raw	raw	JJ
cord-014597-66vd2mdu	148	28	material	material	NN
cord-014597-66vd2mdu	148	29	in	in	IN
cord-014597-66vd2mdu	148	30	GMP	GMP	NNP
cord-014597-66vd2mdu	148	31	processes	process	NNS
cord-014597-66vd2mdu	148	32	for	for	IN
cord-014597-66vd2mdu	148	33	the	the	DT
cord-014597-66vd2mdu	148	34	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	148	35	of	of	IN
cord-014597-66vd2mdu	148	36	clinical	clinical	JJ
cord-014597-66vd2mdu	148	37	batches	batch	NNS
cord-014597-66vd2mdu	148	38	of	of	IN
cord-014597-66vd2mdu	148	39	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	148	40	products	product	NNS
cord-014597-66vd2mdu	148	41	.	.	.
cord-014597-66vd2mdu	149	1	Lentivirus	Lentivirus	NNP
cord-014597-66vd2mdu	149	2	and	and	CC
cord-014597-66vd2mdu	149	3	AAV	AAV	NNP
cord-014597-66vd2mdu	149	4	production	production	NN
cord-014597-66vd2mdu	149	5	.	.	.
cord-014597-66vd2mdu	150	1	Irrespective	irrespective	RB
cord-014597-66vd2mdu	150	2	of	of	IN
cord-014597-66vd2mdu	150	3	the	the	DT
cord-014597-66vd2mdu	150	4	cell	cell	NN
cord-014597-66vd2mdu	150	5	culture	culture	NN
cord-014597-66vd2mdu	150	6	vessel	vessel	NN
cord-014597-66vd2mdu	150	7	type	type	NN
cord-014597-66vd2mdu	150	8	,	,	,
cord-014597-66vd2mdu	150	9	transfection	transfection	NN
cord-014597-66vd2mdu	150	10	using	use	VBG
cord-014597-66vd2mdu	150	11	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	150	12	®	®	NNP
cord-014597-66vd2mdu	150	13	was	be	VBD
cord-014597-66vd2mdu	150	14	performed	perform	VBN
cord-014597-66vd2mdu	150	15	following	follow	VBG
cord-014597-66vd2mdu	150	16	our	-PRON-	PRP$
cord-014597-66vd2mdu	150	17	recommandations	recommandation	NNS
cord-014597-66vd2mdu	150	18	.	.	.
cord-014597-66vd2mdu	151	1	As	as	IN
cord-014597-66vd2mdu	151	2	an	an	DT
cord-014597-66vd2mdu	151	3	example	example	NN
cord-014597-66vd2mdu	151	4	,	,	,
cord-014597-66vd2mdu	151	5	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	151	6	T	T	NNP
cord-014597-66vd2mdu	151	7	(	(	-LRB-
cord-014597-66vd2mdu	151	8	lentivirus	lentivirus	NN
cord-014597-66vd2mdu	151	9	)	)	-RRB-
cord-014597-66vd2mdu	151	10	and	and	CC
cord-014597-66vd2mdu	151	11	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	151	12	(	(	-LRB-
cord-014597-66vd2mdu	151	13	AAV	AAV	NNP
cord-014597-66vd2mdu	151	14	)	)	-RRB-
cord-014597-66vd2mdu	151	15	cells	cell	NNS
cord-014597-66vd2mdu	151	16	were	be	VBD
cord-014597-66vd2mdu	151	17	thawed	thaw	VBN
cord-014597-66vd2mdu	151	18	directly	directly	RB
cord-014597-66vd2mdu	151	19	into	into	IN
cord-014597-66vd2mdu	151	20	each	each	DT
cord-014597-66vd2mdu	151	21	medium	medium	NN
cord-014597-66vd2mdu	151	22	and	and	CC
cord-014597-66vd2mdu	151	23	passaged	passage	VBD
cord-014597-66vd2mdu	151	24	every	every	DT
cord-014597-66vd2mdu	151	25	3	3	CD
cord-014597-66vd2mdu	151	26	to	to	TO
cord-014597-66vd2mdu	151	27	4	4	CD
cord-014597-66vd2mdu	151	28	days	day	NNS
cord-014597-66vd2mdu	151	29	before	before	IN
cord-014597-66vd2mdu	151	30	going	go	VBG
cord-014597-66vd2mdu	151	31	into	into	IN
cord-014597-66vd2mdu	151	32	a	a	DT
cord-014597-66vd2mdu	151	33	2	2	CD
cord-014597-66vd2mdu	151	34	Liter	liter	NN
cord-014597-66vd2mdu	151	35	benchtop	benchtop	NN
cord-014597-66vd2mdu	151	36	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	151	37	.	.	.
cord-014597-66vd2mdu	152	1	Cells	cell	NNS
cord-014597-66vd2mdu	152	2	were	be	VBD
cord-014597-66vd2mdu	152	3	resuspended	resuspend	VBN
cord-014597-66vd2mdu	152	4	and	and	CC
cord-014597-66vd2mdu	152	5	cultured	culture	VBN
cord-014597-66vd2mdu	152	6	for	for	IN
cord-014597-66vd2mdu	152	7	3	3	CD
cord-014597-66vd2mdu	152	8	days	day	NNS
cord-014597-66vd2mdu	152	9	before	before	IN
cord-014597-66vd2mdu	152	10	transfection	transfection	NN
cord-014597-66vd2mdu	152	11	with	with	IN
cord-014597-66vd2mdu	152	12	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	152	13	®	®	NNPS
cord-014597-66vd2mdu	152	14	.	.	.
cord-014597-66vd2mdu	153	1	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	154	1	T	t	NN
cord-014597-66vd2mdu	154	2	cells	cell	NNS
cord-014597-66vd2mdu	154	3	were	be	VBD
cord-014597-66vd2mdu	154	4	transfected	transfecte	VBN
cord-014597-66vd2mdu	154	5	with	with	IN
cord-014597-66vd2mdu	154	6	a	a	DT
cord-014597-66vd2mdu	154	7	third	third	JJ
cord-014597-66vd2mdu	154	8	-	-	HYPH
cord-014597-66vd2mdu	154	9	generation	generation	NN
cord-014597-66vd2mdu	154	10	system	system	NN
cord-014597-66vd2mdu	154	11	(	(	-LRB-
cord-014597-66vd2mdu	154	12	four	four	CD
cord-014597-66vd2mdu	154	13	plasmids	plasmid	NNS
cord-014597-66vd2mdu	154	14	)	)	-RRB-
cord-014597-66vd2mdu	154	15	for	for	IN
cord-014597-66vd2mdu	154	16	lentivirus	lentivirus	NN
cord-014597-66vd2mdu	154	17	production	production	NN
cord-014597-66vd2mdu	154	18	.	.	.
cord-014597-66vd2mdu	155	1	HEK-293	hek-293	JJ
cord-014597-66vd2mdu	155	2	cells	cell	NNS
cord-014597-66vd2mdu	155	3	were	be	VBD
cord-014597-66vd2mdu	155	4	co	co	JJ
cord-014597-66vd2mdu	155	5	-	-	VBN
cord-014597-66vd2mdu	155	6	transfected	transfecte	VBN
cord-014597-66vd2mdu	155	7	with	with	IN
cord-014597-66vd2mdu	155	8	three	three	CD
cord-014597-66vd2mdu	155	9	plasmids	plasmid	NNS
cord-014597-66vd2mdu	155	10	for	for	IN
cord-014597-66vd2mdu	155	11	AAV	AAV	NNP
cord-014597-66vd2mdu	155	12	production	production	NN
cord-014597-66vd2mdu	155	13	.	.	.
cord-014597-66vd2mdu	156	1	Lentiviral	Lentiviral	NNP
cord-014597-66vd2mdu	156	2	and	and	CC
cord-014597-66vd2mdu	156	3	AAV	AAV	NNP
cord-014597-66vd2mdu	156	4	titers	titer	NNS
cord-014597-66vd2mdu	156	5	were	be	VBD
cord-014597-66vd2mdu	156	6	measured	measure	VBN
cord-014597-66vd2mdu	156	7	48	48	CD
cord-014597-66vd2mdu	156	8	and	and	CC
cord-014597-66vd2mdu	156	9	72	72	CD
cord-014597-66vd2mdu	156	10	hours	hour	NNS
cord-014597-66vd2mdu	156	11	post	post	NN
cord-014597-66vd2mdu	156	12	-	-	NN
cord-014597-66vd2mdu	156	13	transfection	transfection	JJ
cord-014597-66vd2mdu	157	1	(	(	-LRB-
cord-014597-66vd2mdu	157	2	Data	Data	NNP
cord-014597-66vd2mdu	157	3	kindly	kindly	RB
cord-014597-66vd2mdu	157	4	provided	provide	VBN
cord-014597-66vd2mdu	157	5	by	by	IN
cord-014597-66vd2mdu	157	6	Généthon	Généthon	NNP
cord-014597-66vd2mdu	157	7	)	)	-RRB-
cord-014597-66vd2mdu	157	8	.	.	.
cord-014597-66vd2mdu	158	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	159	1	®	®	NNP
cord-014597-66vd2mdu	159	2	is	be	VBZ
cord-014597-66vd2mdu	159	3	the	the	DT
cord-014597-66vd2mdu	159	4	reagent	reagent	NN
cord-014597-66vd2mdu	159	5	of	of	IN
cord-014597-66vd2mdu	159	6	choice	choice	NN
cord-014597-66vd2mdu	159	7	for	for	IN
cord-014597-66vd2mdu	159	8	virus	virus	NN
cord-014597-66vd2mdu	159	9	production	production	NN
cord-014597-66vd2mdu	159	10	runs	run	VBZ
cord-014597-66vd2mdu	159	11	in	in	IN
cord-014597-66vd2mdu	159	12	most	most	RBS
cord-014597-66vd2mdu	159	13	adherent	adherent	JJ
cord-014597-66vd2mdu	159	14	and	and	CC
cord-014597-66vd2mdu	159	15	suspension	suspension	NN
cord-014597-66vd2mdu	159	16	cell	cell	NN
cord-014597-66vd2mdu	159	17	culture	culture	NN
cord-014597-66vd2mdu	159	18	systems	system	NNS
cord-014597-66vd2mdu	159	19	from	from	IN
cord-014597-66vd2mdu	159	20	process	process	NN
cord-014597-66vd2mdu	159	21	development	development	NN
cord-014597-66vd2mdu	159	22	up	up	IN
cord-014597-66vd2mdu	159	23	to	to	IN
cord-014597-66vd2mdu	159	24	large	large	JJ
cord-014597-66vd2mdu	159	25	scale	scale	NN
cord-014597-66vd2mdu	159	26	clinical	clinical	JJ
cord-014597-66vd2mdu	159	27	-	-	HYPH
cord-014597-66vd2mdu	159	28	grade	grade	NN
cord-014597-66vd2mdu	159	29	virus	virus	NN
cord-014597-66vd2mdu	159	30	production	production	NN
cord-014597-66vd2mdu	159	31	.	.	.
cord-014597-66vd2mdu	160	1	Irrespective	irrespective	RB
cord-014597-66vd2mdu	160	2	of	of	IN
cord-014597-66vd2mdu	160	3	the	the	DT
cord-014597-66vd2mdu	160	4	cell	cell	NN
cord-014597-66vd2mdu	160	5	culture	culture	NN
cord-014597-66vd2mdu	160	6	-	-	HYPH
cord-014597-66vd2mdu	160	7	based	base	VBN
cord-014597-66vd2mdu	160	8	system	system	NN
cord-014597-66vd2mdu	160	9	and	and	CC
cord-014597-66vd2mdu	160	10	production	production	NN
cord-014597-66vd2mdu	160	11	scale	scale	NN
cord-014597-66vd2mdu	160	12	,	,	,
cord-014597-66vd2mdu	161	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	161	2	®	®	VBP
cord-014597-66vd2mdu	161	3	and	and	CC
cord-014597-66vd2mdu	161	4	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	161	5	®	®	NNPS
cord-014597-66vd2mdu	161	6	-HQ	-hq	RB
cord-014597-66vd2mdu	161	7	have	have	VBP
cord-014597-66vd2mdu	161	8	led	lead	VBN
cord-014597-66vd2mdu	161	9	to	to	IN
cord-014597-66vd2mdu	161	10	efficient	efficient	JJ
cord-014597-66vd2mdu	161	11	viral	viral	JJ
cord-014597-66vd2mdu	161	12	vector	vector	NN
cord-014597-66vd2mdu	161	13	yields	yield	NNS
cord-014597-66vd2mdu	161	14	in	in	IN
cord-014597-66vd2mdu	161	15	standard	standard	JJ
cord-014597-66vd2mdu	161	16	laboratory	laboratory	NN
cord-014597-66vd2mdu	161	17	cell	cell	NN
cord-014597-66vd2mdu	161	18	systems	system	NNS
cord-014597-66vd2mdu	161	19	,	,	,
cord-014597-66vd2mdu	161	20	such	such	JJ
cord-014597-66vd2mdu	161	21	as	as	IN
cord-014597-66vd2mdu	161	22	in	in	IN
cord-014597-66vd2mdu	161	23	flasks	flask	NNS
cord-014597-66vd2mdu	161	24	,	,	,
cord-014597-66vd2mdu	161	25	cell	cell	NN
cord-014597-66vd2mdu	161	26	factories	factory	NNS
cord-014597-66vd2mdu	161	27	,	,	,
cord-014597-66vd2mdu	161	28	and	and	CC
cord-014597-66vd2mdu	161	29	roller	roller	NN
cord-014597-66vd2mdu	161	30	bottles	bottle	NNS
cord-014597-66vd2mdu	161	31	,	,	,
cord-014597-66vd2mdu	161	32	as	as	RB
cord-014597-66vd2mdu	161	33	well	well	RB
cord-014597-66vd2mdu	161	34	as	as	IN
cord-014597-66vd2mdu	161	35	in	in	IN
cord-014597-66vd2mdu	161	36	multilayers	multilayer	NNS
cord-014597-66vd2mdu	161	37	flasks	flask	NNS
cord-014597-66vd2mdu	161	38	or	or	CC
cord-014597-66vd2mdu	161	39	fixed	fix	VBN
cord-014597-66vd2mdu	161	40	-	-	HYPH
cord-014597-66vd2mdu	161	41	bed	bed	NN
cord-014597-66vd2mdu	161	42	culture	culture	NN
cord-014597-66vd2mdu	161	43	systems	system	NNS
cord-014597-66vd2mdu	161	44	that	that	WDT
cord-014597-66vd2mdu	161	45	take	take	VBP
cord-014597-66vd2mdu	161	46	into	into	IN
cord-014597-66vd2mdu	161	47	account	account	NN
cord-014597-66vd2mdu	161	48	time	time	NN
cord-014597-66vd2mdu	161	49	and	and	CC
cord-014597-66vd2mdu	161	50	space	space	NN
cord-014597-66vd2mdu	161	51	concerns	concern	NNS
cord-014597-66vd2mdu	161	52	for	for	IN
cord-014597-66vd2mdu	161	53	the	the	DT
cord-014597-66vd2mdu	161	54	scaling	scale	VBG
cord-014597-66vd2mdu	161	55	-	-	HYPH
cord-014597-66vd2mdu	161	56	up	up	RP
cord-014597-66vd2mdu	161	57	process	process	NN
cord-014597-66vd2mdu	161	58	(	(	-LRB-
cord-014597-66vd2mdu	161	59	Table	table	NN
cord-014597-66vd2mdu	161	60	1	1	CD
cord-014597-66vd2mdu	161	61	)	)	-RRB-
cord-014597-66vd2mdu	161	62	.	.	.
cord-014597-66vd2mdu	162	1	For	for	IN
cord-014597-66vd2mdu	162	2	example	example	NN
cord-014597-66vd2mdu	162	3	,	,	,
cord-014597-66vd2mdu	162	4	high	high	JJ
cord-014597-66vd2mdu	162	5	viral	viral	JJ
cord-014597-66vd2mdu	162	6	vector	vector	NN
cord-014597-66vd2mdu	162	7	yields	yield	NNS
cord-014597-66vd2mdu	162	8	superior	superior	JJ
cord-014597-66vd2mdu	162	9	to	to	IN
cord-014597-66vd2mdu	162	10	10	10	CD
cord-014597-66vd2mdu	162	11	7	7	CD
cord-014597-66vd2mdu	162	12	IG	ig	NN
cord-014597-66vd2mdu	162	13	/	/	SYM
cord-014597-66vd2mdu	162	14	mL	ml	NN
cord-014597-66vd2mdu	162	15	and	and	CC
cord-014597-66vd2mdu	162	16	10	10	CD
cord-014597-66vd2mdu	162	17	11	11	CD
cord-014597-66vd2mdu	162	18	-10	-10	CD
cord-014597-66vd2mdu	162	19	12	12	CD
cord-014597-66vd2mdu	162	20	VG	VG	NNP
cord-014597-66vd2mdu	162	21	/	/	SYM
cord-014597-66vd2mdu	162	22	mL	ml	NN
cord-014597-66vd2mdu	162	23	were	be	VBD
cord-014597-66vd2mdu	162	24	obtained	obtain	VBN
cord-014597-66vd2mdu	162	25	respectively	respectively	RB
cord-014597-66vd2mdu	162	26	for	for	IN
cord-014597-66vd2mdu	162	27	lentiviruses	lentiviruse	NNS
cord-014597-66vd2mdu	162	28	and	and	CC
cord-014597-66vd2mdu	162	29	AAVs	aav	NNS
cord-014597-66vd2mdu	162	30	in	in	IN
cord-014597-66vd2mdu	162	31	suspension	suspension	NN
cord-014597-66vd2mdu	162	32	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	162	33	T	T	NNP
cord-014597-66vd2mdu	162	34	and	and	CC
cord-014597-66vd2mdu	162	35	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	162	36	cells	cell	NNS
cord-014597-66vd2mdu	162	37	cultured	culture	VBN
cord-014597-66vd2mdu	162	38	in	in	IN
cord-014597-66vd2mdu	162	39	one	one	CD
cord-014597-66vd2mdu	162	40	of	of	IN
cord-014597-66vd2mdu	162	41	the	the	DT
cord-014597-66vd2mdu	162	42	commercially	commercially	RB
cord-014597-66vd2mdu	162	43	available	available	JJ
cord-014597-66vd2mdu	162	44	synthetic	synthetic	JJ
cord-014597-66vd2mdu	162	45	cell	cell	NN
cord-014597-66vd2mdu	162	46	culture	culture	NN
cord-014597-66vd2mdu	162	47	medium	medium	NN
cord-014597-66vd2mdu	162	48	BalanCD	BalanCD	NNP
cord-014597-66vd2mdu	162	49	®	®	POS
cord-014597-66vd2mdu	162	50	HEK293	HEK293	NNP
cord-014597-66vd2mdu	163	1	(	(	-LRB-
cord-014597-66vd2mdu	163	2	Irvine	Irvine	NNP
cord-014597-66vd2mdu	163	3	Scientific	Scientific	NNP
cord-014597-66vd2mdu	163	4	®	®	NNP
cord-014597-66vd2mdu	163	5	)	)	-RRB-
cord-014597-66vd2mdu	163	6	.	.	.
cord-014597-66vd2mdu	164	1	Conclusion	Conclusion	NNP
cord-014597-66vd2mdu	164	2	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	164	3	®	®	NN
cord-014597-66vd2mdu	164	4	and	and	CC
cord-014597-66vd2mdu	164	5	its	-PRON-	PRP$
cord-014597-66vd2mdu	164	6	higher	high	JJR
cord-014597-66vd2mdu	164	7	quality	quality	NN
cord-014597-66vd2mdu	164	8	grade	grade	NN
cord-014597-66vd2mdu	165	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	166	1	®	®	,
cord-014597-66vd2mdu	166	2	-HQ	-hq	CC
cord-014597-66vd2mdu	166	3	are	be	VBP
cord-014597-66vd2mdu	166	4	the	the	DT
cord-014597-66vd2mdu	166	5	unique	unique	JJ
cord-014597-66vd2mdu	166	6	PEI	PEI	NNP
cord-014597-66vd2mdu	166	7	suitable	suitable	JJ
cord-014597-66vd2mdu	166	8	for	for	IN
cord-014597-66vd2mdu	166	9	efficient	efficient	JJ
cord-014597-66vd2mdu	166	10	and	and	CC
cord-014597-66vd2mdu	166	11	reproducible	reproducible	JJ
cord-014597-66vd2mdu	166	12	production	production	NN
cord-014597-66vd2mdu	166	13	of	of	IN
cord-014597-66vd2mdu	166	14	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	166	15	viral	viral	JJ
cord-014597-66vd2mdu	166	16	vectors	vector	NNS
cord-014597-66vd2mdu	166	17	.	.	.
cord-014597-66vd2mdu	167	1	Efficient	efficient	JJ
cord-014597-66vd2mdu	167	2	viral	viral	JJ
cord-014597-66vd2mdu	167	3	vector	vector	NN
cord-014597-66vd2mdu	167	4	production	production	NN
cord-014597-66vd2mdu	167	5	yields	yield	NNS
cord-014597-66vd2mdu	167	6	can	can	MD
cord-014597-66vd2mdu	167	7	be	be	VB
cord-014597-66vd2mdu	167	8	achieved	achieve	VBN
cord-014597-66vd2mdu	167	9	in	in	IN
cord-014597-66vd2mdu	167	10	most	most	JJS
cord-014597-66vd2mdu	167	11	cell	cell	NN
cord-014597-66vd2mdu	167	12	culture	culture	NN
cord-014597-66vd2mdu	167	13	systems	system	NNS
cord-014597-66vd2mdu	167	14	,	,	,
cord-014597-66vd2mdu	167	15	irrespective	irrespective	RB
cord-014597-66vd2mdu	167	16	of	of	IN
cord-014597-66vd2mdu	167	17	the	the	DT
cord-014597-66vd2mdu	167	18	production	production	NN
cord-014597-66vd2mdu	167	19	scale	scale	NN
cord-014597-66vd2mdu	167	20	.	.	.
cord-014597-66vd2mdu	168	1	With	with	IN
cord-014597-66vd2mdu	168	2	appropriate	appropriate	JJ
cord-014597-66vd2mdu	168	3	and	and	CC
cord-014597-66vd2mdu	168	4	advanced	advanced	JJ
cord-014597-66vd2mdu	168	5	quality	quality	NN
cord-014597-66vd2mdu	168	6	controls	control	NNS
cord-014597-66vd2mdu	168	7	,	,	,
cord-014597-66vd2mdu	168	8	the	the	DT
cord-014597-66vd2mdu	168	9	highest	high	JJS
cord-014597-66vd2mdu	168	10	quality	quality	NN
cord-014597-66vd2mdu	168	11	grade	grade	NN
cord-014597-66vd2mdu	168	12	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	168	13	®	®	NNPS
cord-014597-66vd2mdu	168	14	-HQ	-hq	NN
cord-014597-66vd2mdu	168	15	is	be	VBZ
cord-014597-66vd2mdu	168	16	commercially	commercially	RB
cord-014597-66vd2mdu	168	17	available	available	JJ
cord-014597-66vd2mdu	168	18	to	to	TO
cord-014597-66vd2mdu	168	19	accompany	accompany	VB
cord-014597-66vd2mdu	168	20	academics	academic	NNS
cord-014597-66vd2mdu	168	21	and	and	CC
cord-014597-66vd2mdu	168	22	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	168	23	companies	company	NNS
cord-014597-66vd2mdu	168	24	in	in	IN
cord-014597-66vd2mdu	168	25	terms	term	NNS
cord-014597-66vd2mdu	168	26	of	of	IN
cord-014597-66vd2mdu	168	27	qualified	qualified	JJ
cord-014597-66vd2mdu	168	28	raw	raw	JJ
cord-014597-66vd2mdu	168	29	material	material	NN
cord-014597-66vd2mdu	168	30	for	for	IN
cord-014597-66vd2mdu	168	31	their	-PRON-	PRP$
cord-014597-66vd2mdu	168	32	GMP	GMP	NNP
cord-014597-66vd2mdu	168	33	-	-	HYPH
cord-014597-66vd2mdu	168	34	grade	grade	NN
cord-014597-66vd2mdu	168	35	viral	viral	JJ
cord-014597-66vd2mdu	168	36	vector	vector	NN
cord-014597-66vd2mdu	168	37	production	production	NN
cord-014597-66vd2mdu	168	38	needs	need	NNS
cord-014597-66vd2mdu	168	39	.	.	.
cord-014597-66vd2mdu	169	1	b	b	LS
cord-014597-66vd2mdu	169	2	Distinction	distinction	NN
cord-014597-66vd2mdu	169	3	between	between	IN
cord-014597-66vd2mdu	169	4	Fc	Fc	NNP
cord-014597-66vd2mdu	169	5	and	and	CC
cord-014597-66vd2mdu	169	6	Fab	fab	JJ
cord-014597-66vd2mdu	169	7	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	169	8	in	in	IN
cord-014597-66vd2mdu	169	9	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	169	10	.	.	.
cord-014597-66vd2mdu	170	1	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	170	2	was	be	VBD
cord-014597-66vd2mdu	170	3	diluted	dilute	VBN
cord-014597-66vd2mdu	170	4	to	to	IN
cord-014597-66vd2mdu	170	5	a	a	DT
cord-014597-66vd2mdu	170	6	concentration	concentration	NN
cord-014597-66vd2mdu	170	7	of	of	IN
cord-014597-66vd2mdu	170	8	200	200	CD
cord-014597-66vd2mdu	170	9	μg	μg	NN
cord-014597-66vd2mdu	170	10	/	/	SYM
cord-014597-66vd2mdu	170	11	mL	ml	NN
cord-014597-66vd2mdu	170	12	in	in	IN
cord-014597-66vd2mdu	170	13	TRIS	TRIS	NNP
cord-014597-66vd2mdu	170	14	buffer	buffer	NN
cord-014597-66vd2mdu	170	15	and	and	CC
cord-014597-66vd2mdu	170	16	measured	measure	VBN
cord-014597-66vd2mdu	170	17	in	in	IN
cord-014597-66vd2mdu	170	18	native	native	JJ
cord-014597-66vd2mdu	170	19	and	and	CC
cord-014597-66vd2mdu	170	20	denatured	denatured	JJ
cord-014597-66vd2mdu	170	21	conditions	condition	NNS
cord-014597-66vd2mdu	170	22	to	to	TO
cord-014597-66vd2mdu	170	23	distinguish	distinguish	VB
cord-014597-66vd2mdu	170	24	Fab	fab	JJ
cord-014597-66vd2mdu	170	25	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	170	26	(	(	-LRB-
cord-014597-66vd2mdu	170	27	native	native	JJ
cord-014597-66vd2mdu	170	28	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	170	29	)	)	-RRB-
cord-014597-66vd2mdu	170	30	from	from	IN
cord-014597-66vd2mdu	170	31	Fab	Fab	NNP
cord-014597-66vd2mdu	170	32	and	and	CC
cord-014597-66vd2mdu	170	33	Fc	Fc	NNP
cord-014597-66vd2mdu	170	34	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	170	35	(	(	-LRB-
cord-014597-66vd2mdu	170	36	denatured	denature	VBN
cord-014597-66vd2mdu	170	37	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	170	38	)	)	-RRB-
cord-014597-66vd2mdu	170	39	.	.	.
cord-014597-66vd2mdu	171	1	It	-PRON-	PRP
cord-014597-66vd2mdu	171	2	could	could	MD
cord-014597-66vd2mdu	171	3	be	be	VB
cord-014597-66vd2mdu	171	4	confirmed	confirm	VBN
cord-014597-66vd2mdu	171	5	that	that	IN
cord-014597-66vd2mdu	171	6	sialic	sialic	JJ
cord-014597-66vd2mdu	171	7	acids	acid	NNS
cord-014597-66vd2mdu	171	8	are	be	VBP
cord-014597-66vd2mdu	171	9	almost	almost	RB
cord-014597-66vd2mdu	171	10	exclusively	exclusively	RB
cord-014597-66vd2mdu	171	11	present	present	JJ
cord-014597-66vd2mdu	171	12	on	on	IN
cord-014597-66vd2mdu	171	13	the	the	DT
cord-014597-66vd2mdu	171	14	Fab	fab	JJ
cord-014597-66vd2mdu	171	15	part	part	NN
cord-014597-66vd2mdu	171	16	of	of	IN
cord-014597-66vd2mdu	171	17	Erbitux	Erbitux	NNP
cord-014597-66vd2mdu	171	18	and	and	CC
cord-014597-66vd2mdu	171	19	that	that	IN
cord-014597-66vd2mdu	171	20	the	the	DT
cord-014597-66vd2mdu	171	21	high	high	JJ
cord-014597-66vd2mdu	171	22	mannose	mannose	NN
cord-014597-66vd2mdu	171	23	glycans	glycan	NNS
cord-014597-66vd2mdu	171	24	are	be	VBP
cord-014597-66vd2mdu	171	25	only	only	RB
cord-014597-66vd2mdu	171	26	found	find	VBN
cord-014597-66vd2mdu	171	27	in	in	IN
cord-014597-66vd2mdu	171	28	the	the	DT
cord-014597-66vd2mdu	171	29	Fc	Fc	NNP
cord-014597-66vd2mdu	171	30	part	part	NN
cord-014597-66vd2mdu	171	31	.	.	.
cord-014597-66vd2mdu	172	1	c	c	LS
cord-014597-66vd2mdu	172	2	Lectin	Lectin	NNP
cord-014597-66vd2mdu	172	3	binding	bind	VBG
cord-014597-66vd2mdu	172	4	rates	rate	NNS
cord-014597-66vd2mdu	172	5	correlate	correlate	VBP
cord-014597-66vd2mdu	172	6	with	with	IN
cord-014597-66vd2mdu	172	7	the	the	DT
cord-014597-66vd2mdu	172	8	levels	level	NNS
cord-014597-66vd2mdu	172	9	of	of	IN
cord-014597-66vd2mdu	172	10	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	172	11	and	and	CC
cord-014597-66vd2mdu	172	12	fucosylation	fucosylation	NN
cord-014597-66vd2mdu	172	13	.	.	.
cord-014597-66vd2mdu	173	1	Two	two	CD
cord-014597-66vd2mdu	173	2	glycan	glycan	NN
cord-014597-66vd2mdu	173	3	variants	variant	NNS
cord-014597-66vd2mdu	173	4	samples	sample	NNS
cord-014597-66vd2mdu	173	5	of	of	IN
cord-014597-66vd2mdu	173	6	the	the	DT
cord-014597-66vd2mdu	173	7	same	same	JJ
cord-014597-66vd2mdu	173	8	IgG	igg	NN
cord-014597-66vd2mdu	173	9	from	from	IN
cord-014597-66vd2mdu	173	10	Merck	Merck	NNP
cord-014597-66vd2mdu	173	11	were	be	VBD
cord-014597-66vd2mdu	173	12	mixed	mix	VBN
cord-014597-66vd2mdu	173	13	in	in	IN
cord-014597-66vd2mdu	173	14	different	different	JJ
cord-014597-66vd2mdu	173	15	ratios	ratio	NNS
cord-014597-66vd2mdu	173	16	to	to	TO
cord-014597-66vd2mdu	173	17	yield	yield	VB
cord-014597-66vd2mdu	173	18	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	173	19	rates	rate	NNS
cord-014597-66vd2mdu	173	20	of	of	IN
cord-014597-66vd2mdu	173	21	9	9	CD
cord-014597-66vd2mdu	173	22	to	to	TO
cord-014597-66vd2mdu	173	23	55	55	CD
cord-014597-66vd2mdu	173	24	%	%	NN
cord-014597-66vd2mdu	173	25	in	in	IN
cord-014597-66vd2mdu	173	26	terminal	terminal	NN
cord-014597-66vd2mdu	173	27	β	β	NN
cord-014597-66vd2mdu	173	28	-	-	HYPH
cord-014597-66vd2mdu	173	29	galactose	galactose	NN
cord-014597-66vd2mdu	173	30	and	and	CC
cord-014597-66vd2mdu	173	31	8	8	CD
cord-014597-66vd2mdu	173	32	to	to	TO
cord-014597-66vd2mdu	173	33	100	100	CD
cord-014597-66vd2mdu	173	34	%	%	NN
cord-014597-66vd2mdu	173	35	in	in	IN
cord-014597-66vd2mdu	173	36	core	core	NN
cord-014597-66vd2mdu	173	37	-	-	HYPH
cord-014597-66vd2mdu	173	38	fucose	fucose	JJ
cord-014597-66vd2mdu	173	39	,	,	,
cord-014597-66vd2mdu	173	40	based	base	VBN
cord-014597-66vd2mdu	173	41	on	on	IN
cord-014597-66vd2mdu	173	42	data	datum	NNS
cord-014597-66vd2mdu	173	43	from	from	IN
cord-014597-66vd2mdu	173	44	2-AB	2-ab	CD
cord-014597-66vd2mdu	173	45	UPLC	UPLC	NNP
cord-014597-66vd2mdu	173	46	analysis	analysis	NN
cord-014597-66vd2mdu	173	47	.	.	.
cord-014597-66vd2mdu	174	1	The	the	DT
cord-014597-66vd2mdu	174	2	mixtures	mixture	NNS
cord-014597-66vd2mdu	174	3	all	all	DT
cord-014597-66vd2mdu	174	4	contained	contain	VBD
cord-014597-66vd2mdu	174	5	0.5	0.5	CD
cord-014597-66vd2mdu	174	6	μg	μg	NN
cord-014597-66vd2mdu	174	7	IgG	igg	NN
cord-014597-66vd2mdu	174	8	per	per	IN
cord-014597-66vd2mdu	174	9	well	well	RB
cord-014597-66vd2mdu	174	10	.	.	.
cord-014597-66vd2mdu	175	1	The	the	DT
cord-014597-66vd2mdu	175	2	measured	measured	JJ
cord-014597-66vd2mdu	175	3	lectin	lectin	NN
cord-014597-66vd2mdu	175	4	binding	bind	VBG
cord-014597-66vd2mdu	175	5	rates	rate	NNS
cord-014597-66vd2mdu	175	6	for	for	IN
cord-014597-66vd2mdu	175	7	all	all	DT
cord-014597-66vd2mdu	175	8	galactose	galactose	NN
cord-014597-66vd2mdu	175	9	and	and	CC
cord-014597-66vd2mdu	175	10	fucose	fucose	JJ
cord-014597-66vd2mdu	175	11	markers	marker	NNS
cord-014597-66vd2mdu	175	12	correlate	correlate	VBP
cord-014597-66vd2mdu	175	13	very	very	RB
cord-014597-66vd2mdu	175	14	well	well	RB
cord-014597-66vd2mdu	175	15	with	with	IN
cord-014597-66vd2mdu	175	16	the	the	DT
cord-014597-66vd2mdu	175	17	respective	respective	JJ
cord-014597-66vd2mdu	175	18	degree	degree	NN
cord-014597-66vd2mdu	175	19	of	of	IN
cord-014597-66vd2mdu	175	20	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	175	21	in	in	IN
cord-014597-66vd2mdu	175	22	the	the	DT
cord-014597-66vd2mdu	175	23	mixtures	mixture	NNS
cord-014597-66vd2mdu	175	24	.	.	.
cord-014597-66vd2mdu	176	1	All	all	DT
cord-014597-66vd2mdu	176	2	measurements	measurement	NNS
cord-014597-66vd2mdu	176	3	were	be	VBD
cord-014597-66vd2mdu	176	4	performed	perform	VBN
cord-014597-66vd2mdu	176	5	in	in	IN
cord-014597-66vd2mdu	176	6	triplicates	triplicate	NNS
cord-014597-66vd2mdu	176	7	(	(	-LRB-
cord-014597-66vd2mdu	176	8	Irvine	Irvine	NNP
cord-014597-66vd2mdu	176	9	 	 	_SP
cord-014597-66vd2mdu	176	10	Scientific	Scientific	NNP
cord-014597-66vd2mdu	176	11	®	®	NNP
cord-014597-66vd2mdu	176	12	)	)	-RRB-
cord-014597-66vd2mdu	176	13	.	.	.
cord-014597-66vd2mdu	177	1	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	177	2	T	T	NNP
cord-014597-66vd2mdu	177	3	(	(	-LRB-
cord-014597-66vd2mdu	177	4	lentivirus	lentivirus	NN
cord-014597-66vd2mdu	177	5	)	)	-RRB-
cord-014597-66vd2mdu	177	6	and	and	CC
cord-014597-66vd2mdu	177	7	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	177	8	(	(	-LRB-
cord-014597-66vd2mdu	177	9	AAV	AAV	NNP
cord-014597-66vd2mdu	177	10	)	)	-RRB-
cord-014597-66vd2mdu	177	11	cells	cell	NNS
cord-014597-66vd2mdu	177	12	were	be	VBD
cord-014597-66vd2mdu	177	13	thawed	thaw	VBN
cord-014597-66vd2mdu	177	14	directly	directly	RB
cord-014597-66vd2mdu	177	15	into	into	IN
cord-014597-66vd2mdu	177	16	each	each	DT
cord-014597-66vd2mdu	177	17	medium	medium	NN
cord-014597-66vd2mdu	177	18	and	and	CC
cord-014597-66vd2mdu	177	19	passaged	passage	VBD
cord-014597-66vd2mdu	177	20	every	every	DT
cord-014597-66vd2mdu	177	21	3	3	CD
cord-014597-66vd2mdu	177	22	to	to	TO
cord-014597-66vd2mdu	177	23	4	4	CD
cord-014597-66vd2mdu	177	24	days	day	NNS
cord-014597-66vd2mdu	177	25	before	before	IN
cord-014597-66vd2mdu	177	26	going	go	VBG
cord-014597-66vd2mdu	177	27	into	into	IN
cord-014597-66vd2mdu	177	28	a	a	DT
cord-014597-66vd2mdu	177	29	2	2	CD
cord-014597-66vd2mdu	177	30	Liter	liter	NN
cord-014597-66vd2mdu	177	31	benchtop	benchtop	NN
cord-014597-66vd2mdu	177	32	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	177	33	.	.	.
cord-014597-66vd2mdu	178	1	Cells	cell	NNS
cord-014597-66vd2mdu	178	2	were	be	VBD
cord-014597-66vd2mdu	178	3	seeded	seed	VBN
cord-014597-66vd2mdu	178	4	and	and	CC
cord-014597-66vd2mdu	178	5	cultured	culture	VBN
cord-014597-66vd2mdu	178	6	for	for	IN
cord-014597-66vd2mdu	178	7	3	3	CD
cord-014597-66vd2mdu	178	8	days	day	NNS
cord-014597-66vd2mdu	178	9	before	before	IN
cord-014597-66vd2mdu	178	10	being	be	VBG
cord-014597-66vd2mdu	178	11	transfected	transfecte	VBN
cord-014597-66vd2mdu	178	12	by	by	IN
cord-014597-66vd2mdu	179	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	179	2	®	®	.
cord-014597-66vd2mdu	179	3	(	(	-LRB-
cord-014597-66vd2mdu	179	4	Polyplus	Polyplus	NNP
cord-014597-66vd2mdu	179	5	)	)	-RRB-
cord-014597-66vd2mdu	179	6	.	.	.
cord-014597-66vd2mdu	180	1	For	for	IN
cord-014597-66vd2mdu	180	2	transfection	transfection	NN
cord-014597-66vd2mdu	180	3	,	,	,
cord-014597-66vd2mdu	180	4	four	four	CD
cord-014597-66vd2mdu	180	5	plasmids	plasmid	NNS
cord-014597-66vd2mdu	180	6	were	be	VBD
cord-014597-66vd2mdu	180	7	used	use	VBN
cord-014597-66vd2mdu	180	8	for	for	IN
cord-014597-66vd2mdu	180	9	lentivirus	lentivirus	NN
cord-014597-66vd2mdu	180	10	and	and	CC
cord-014597-66vd2mdu	180	11	three	three	CD
cord-014597-66vd2mdu	180	12	plasmids	plasmid	NNS
cord-014597-66vd2mdu	180	13	were	be	VBD
cord-014597-66vd2mdu	180	14	used	use	VBN
cord-014597-66vd2mdu	180	15	for	for	IN
cord-014597-66vd2mdu	180	16	AAV	AAV	NNP
cord-014597-66vd2mdu	180	17	.	.	.
cord-014597-66vd2mdu	181	1	Lentiviral	Lentiviral	NNP
cord-014597-66vd2mdu	181	2	and	and	CC
cord-014597-66vd2mdu	181	3	AAV	AAV	NNP
cord-014597-66vd2mdu	181	4	titer	titer	NN
cord-014597-66vd2mdu	181	5	were	be	VBD
cord-014597-66vd2mdu	181	6	measured	measure	VBN
cord-014597-66vd2mdu	181	7	48	48	CD
cord-014597-66vd2mdu	181	8	and	and	CC
cord-014597-66vd2mdu	181	9	72	72	CD
cord-014597-66vd2mdu	181	10	hours	hour	NNS
cord-014597-66vd2mdu	181	11	post	post	NN
cord-014597-66vd2mdu	181	12	transfection	transfection	NN
cord-014597-66vd2mdu	182	1	(	(	-LRB-
cord-014597-66vd2mdu	182	2	Data	Data	NNP
cord-014597-66vd2mdu	182	3	kindly	kindly	RB
cord-014597-66vd2mdu	182	4	provided	provide	VBN
cord-014597-66vd2mdu	182	5	by	by	IN
cord-014597-66vd2mdu	182	6	Généthon	Généthon	NNP
cord-014597-66vd2mdu	182	7	)	)	-RRB-
cord-014597-66vd2mdu	182	8	Table	table	NN
cord-014597-66vd2mdu	182	9	1	1	CD
cord-014597-66vd2mdu	182	10	(	(	-LRB-
cord-014597-66vd2mdu	182	11	abstract	abstract	JJ
cord-014597-66vd2mdu	182	12	P-004	P-004	NNP
cord-014597-66vd2mdu	182	13	)	)	-RRB-
cord-014597-66vd2mdu	182	14	.	.	.
cord-014597-66vd2mdu	183	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	184	1	®	®	VB
cord-014597-66vd2mdu	184	2	,	,	,
cord-014597-66vd2mdu	184	3	the	the	DT
cord-014597-66vd2mdu	184	4	reagent	reagent	NN
cord-014597-66vd2mdu	184	5	of	of	IN
cord-014597-66vd2mdu	184	6	choice	choice	NN
cord-014597-66vd2mdu	184	7	for	for	IN
cord-014597-66vd2mdu	184	8	virus	virus	NN
cord-014597-66vd2mdu	184	9	production	production	NN
cord-014597-66vd2mdu	184	10	runs	run	VBZ
cord-014597-66vd2mdu	184	11	in	in	IN
cord-014597-66vd2mdu	184	12	most	most	JJS
cord-014597-66vd2mdu	184	13	cell	cell	NN
cord-014597-66vd2mdu	184	14	culture	culture	NN
cord-014597-66vd2mdu	184	15	systems	system	NNS
cord-014597-66vd2mdu	184	16	in	in	IN
cord-014597-66vd2mdu	184	17	both	both	CC
cord-014597-66vd2mdu	184	18	adherent	adherent	JJ
cord-014597-66vd2mdu	184	19	and	and	CC
cord-014597-66vd2mdu	184	20	suspension	suspension	NN
cord-014597-66vd2mdu	184	21	cells	cell	NNS
cord-014597-66vd2mdu	184	22	.	.	.
cord-014597-66vd2mdu	185	1	Irrespective	irrespective	RB
cord-014597-66vd2mdu	185	2	of	of	IN
cord-014597-66vd2mdu	185	3	the	the	DT
cord-014597-66vd2mdu	185	4	cell	cell	NN
cord-014597-66vd2mdu	185	5	culture	culture	NN
cord-014597-66vd2mdu	185	6	-	-	HYPH
cord-014597-66vd2mdu	185	7	based	base	VBN
cord-014597-66vd2mdu	185	8	system	system	NN
cord-014597-66vd2mdu	185	9	and	and	CC
cord-014597-66vd2mdu	185	10	production	production	NN
cord-014597-66vd2mdu	185	11	scale	scale	NN
cord-014597-66vd2mdu	185	12	,	,	,
cord-014597-66vd2mdu	186	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	186	2	®	®	VBP
cord-014597-66vd2mdu	186	3	and	and	CC
cord-014597-66vd2mdu	186	4	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	186	5	®	®	NNPS
cord-014597-66vd2mdu	186	6	-HQ	-hq	RB
cord-014597-66vd2mdu	186	7	have	have	VBP
cord-014597-66vd2mdu	186	8	led	lead	VBN
cord-014597-66vd2mdu	186	9	to	to	IN
cord-014597-66vd2mdu	186	10	efficient	efficient	JJ
cord-014597-66vd2mdu	186	11	viral	viral	JJ
cord-014597-66vd2mdu	186	12	vector	vector	NN
cord-014597-66vd2mdu	186	13	yields	yield	NNS
cord-014597-66vd2mdu	186	14	superior	superior	JJ
cord-014597-66vd2mdu	186	15	to	to	IN
cord-014597-66vd2mdu	186	16	10	10	CD
cord-014597-66vd2mdu	186	17	7	7	CD
cord-014597-66vd2mdu	186	18	IG	ig	NN
cord-014597-66vd2mdu	186	19	/	/	SYM
cord-014597-66vd2mdu	186	20	mL	ml	NN
cord-014597-66vd2mdu	186	21	and	and	CC
cord-014597-66vd2mdu	186	22	10	10	CD
cord-014597-66vd2mdu	186	23	9	9	CD
cord-014597-66vd2mdu	186	24	VG	VG	NNP
cord-014597-66vd2mdu	186	25	/	/	SYM
cord-014597-66vd2mdu	186	26	mL	ml	NN
cord-014597-66vd2mdu	186	27	,	,	,
cord-014597-66vd2mdu	186	28	respectively	respectively	RB
cord-014597-66vd2mdu	186	29	for	for	IN
cord-014597-66vd2mdu	186	30	lentiviruses	lentiviruse	NNS
cord-014597-66vd2mdu	186	31	and	and	CC
cord-014597-66vd2mdu	186	32	AAVs	AAVs	NNPS
cord-014597-66vd2mdu	186	33	Background	Background	NNP
cord-014597-66vd2mdu	186	34	Continuous	continuous	JJ
cord-014597-66vd2mdu	186	35	perfusion	perfusion	NN
cord-014597-66vd2mdu	186	36	process	process	NN
cord-014597-66vd2mdu	186	37	is	be	VBZ
cord-014597-66vd2mdu	186	38	making	make	VBG
cord-014597-66vd2mdu	186	39	a	a	DT
cord-014597-66vd2mdu	186	40	comeback	comeback	NN
cord-014597-66vd2mdu	186	41	as	as	IN
cord-014597-66vd2mdu	186	42	a	a	DT
cord-014597-66vd2mdu	186	43	competing	compete	VBG
cord-014597-66vd2mdu	186	44	upstream	upstream	JJ
cord-014597-66vd2mdu	186	45	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	186	46	technology	technology	NN
cord-014597-66vd2mdu	186	47	for	for	IN
cord-014597-66vd2mdu	186	48	the	the	DT
cord-014597-66vd2mdu	186	49	production	production	NN
cord-014597-66vd2mdu	186	50	of	of	IN
cord-014597-66vd2mdu	186	51	Biopharmaceuticals	Biopharmaceuticals	NNPS
cord-014597-66vd2mdu	186	52	compared	compare	VBN
cord-014597-66vd2mdu	186	53	to	to	IN
cord-014597-66vd2mdu	186	54	the	the	DT
cord-014597-66vd2mdu	186	55	standard	standard	JJ
cord-014597-66vd2mdu	186	56	fed	fed	NNP
cord-014597-66vd2mdu	186	57	batch	batch	NN
cord-014597-66vd2mdu	186	58	processes	process	NNS
cord-014597-66vd2mdu	186	59	.	.	.
cord-014597-66vd2mdu	187	1	This	this	DT
cord-014597-66vd2mdu	187	2	is	be	VBZ
cord-014597-66vd2mdu	187	3	primarily	primarily	RB
cord-014597-66vd2mdu	187	4	because	because	IN
cord-014597-66vd2mdu	187	5	of	of	IN
cord-014597-66vd2mdu	187	6	cost	cost	NN
cord-014597-66vd2mdu	187	7	advantages	advantage	NNS
cord-014597-66vd2mdu	187	8	such	such	JJ
cord-014597-66vd2mdu	187	9	as	as	IN
cord-014597-66vd2mdu	187	10	reduced	reduce	VBN
cord-014597-66vd2mdu	187	11	capital	capital	NN
cord-014597-66vd2mdu	187	12	cost	cost	NN
cord-014597-66vd2mdu	187	13	and	and	CC
cord-014597-66vd2mdu	187	14	increased	increase	VBN
cord-014597-66vd2mdu	187	15	product	product	NN
cord-014597-66vd2mdu	187	16	yield	yield	NN
cord-014597-66vd2mdu	187	17	.	.	.
cord-014597-66vd2mdu	188	1	The	the	DT
cord-014597-66vd2mdu	188	2	change	change	NN
cord-014597-66vd2mdu	188	3	in	in	IN
cord-014597-66vd2mdu	188	4	status	status	NN
cord-014597-66vd2mdu	188	5	of	of	IN
cord-014597-66vd2mdu	188	6	perfusion	perfusion	NN
cord-014597-66vd2mdu	188	7	process	process	NN
cord-014597-66vd2mdu	188	8	from	from	IN
cord-014597-66vd2mdu	188	9	older	old	JJR
cord-014597-66vd2mdu	188	10	perfusion	perfusion	NN
cord-014597-66vd2mdu	188	11	to	to	IN
cord-014597-66vd2mdu	188	12	the	the	DT
cord-014597-66vd2mdu	188	13	new	new	JJ
cord-014597-66vd2mdu	188	14	-	-	HYPH
cord-014597-66vd2mdu	188	15	age	age	NN
cord-014597-66vd2mdu	188	16	perfusion	perfusion	NN
cord-014597-66vd2mdu	188	17	is	be	VBZ
cord-014597-66vd2mdu	188	18	due	due	JJ
cord-014597-66vd2mdu	188	19	to	to	IN
cord-014597-66vd2mdu	188	20	availability	availability	NN
cord-014597-66vd2mdu	188	21	of	of	IN
cord-014597-66vd2mdu	188	22	better	well	JJR
cord-014597-66vd2mdu	188	23	cell	cell	NN
cord-014597-66vd2mdu	188	24	retention	retention	NN
cord-014597-66vd2mdu	188	25	devices	device	NNS
cord-014597-66vd2mdu	188	26	leading	lead	VBG
cord-014597-66vd2mdu	188	27	to	to	IN
cord-014597-66vd2mdu	188	28	more	more	RBR
cord-014597-66vd2mdu	188	29	efficient	efficient	JJ
cord-014597-66vd2mdu	188	30	processes	process	NNS
cord-014597-66vd2mdu	188	31	,	,	,
cord-014597-66vd2mdu	188	32	improved	improved	JJ
cord-014597-66vd2mdu	188	33	cell	cell	NN
cord-014597-66vd2mdu	188	34	lines	line	NNS
cord-014597-66vd2mdu	188	35	,	,	,
cord-014597-66vd2mdu	188	36	cell	cell	NN
cord-014597-66vd2mdu	188	37	culture	culture	NN
cord-014597-66vd2mdu	188	38	medium	medium	NN
cord-014597-66vd2mdu	188	39	capable	capable	JJ
cord-014597-66vd2mdu	188	40	of	of	IN
cord-014597-66vd2mdu	188	41	supporting	support	VBG
cord-014597-66vd2mdu	188	42	high	high	JJ
cord-014597-66vd2mdu	188	43	cell	cell	NN
cord-014597-66vd2mdu	188	44	densities	density	NNS
cord-014597-66vd2mdu	188	45	and	and	CC
cord-014597-66vd2mdu	188	46	better	well	JJR
cord-014597-66vd2mdu	188	47	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	188	48	control	control	NN
cord-014597-66vd2mdu	188	49	strategies	strategy	NNS
cord-014597-66vd2mdu	188	50	.	.	.
cord-014597-66vd2mdu	189	1	In	in	IN
cord-014597-66vd2mdu	189	2	this	this	DT
cord-014597-66vd2mdu	189	3	work	work	NN
cord-014597-66vd2mdu	189	4	,	,	,
cord-014597-66vd2mdu	189	5	we	-PRON-	PRP
cord-014597-66vd2mdu	189	6	present	present	VBP
cord-014597-66vd2mdu	189	7	the	the	DT
cord-014597-66vd2mdu	189	8	advantages	advantage	NNS
cord-014597-66vd2mdu	189	9	,	,	,
cord-014597-66vd2mdu	189	10	limitations	limitation	NNS
cord-014597-66vd2mdu	189	11	and	and	CC
cord-014597-66vd2mdu	189	12	challenges	challenge	NNS
cord-014597-66vd2mdu	189	13	of	of	IN
cord-014597-66vd2mdu	189	14	fed	fed	NNP
cord-014597-66vd2mdu	189	15	batch	batch	NN
cord-014597-66vd2mdu	189	16	and	and	CC
cord-014597-66vd2mdu	189	17	perfusion	perfusion	NN
cord-014597-66vd2mdu	189	18	type	type	NN
cord-014597-66vd2mdu	189	19	of	of	IN
cord-014597-66vd2mdu	189	20	processes	process	NNS
cord-014597-66vd2mdu	189	21	through	through	IN
cord-014597-66vd2mdu	189	22	case	case	NN
cord-014597-66vd2mdu	189	23	studies	study	NNS
cord-014597-66vd2mdu	189	24	.	.	.
cord-014597-66vd2mdu	190	1	Table	table	NN
cord-014597-66vd2mdu	190	2	1	1	CD
cord-014597-66vd2mdu	190	3	Results	result	NNS
cord-014597-66vd2mdu	191	1	The	the	DT
cord-014597-66vd2mdu	191	2	Perfusion	perfusion	NN
cord-014597-66vd2mdu	191	3	run	run	NN
cord-014597-66vd2mdu	191	4	yielded	yield	VBD
cord-014597-66vd2mdu	191	5	5-fold	5-fold	CD
cord-014597-66vd2mdu	191	6	higher	high	JJR
cord-014597-66vd2mdu	191	7	titer	titer	NN
cord-014597-66vd2mdu	191	8	compared	compare	VBN
cord-014597-66vd2mdu	191	9	to	to	IN
cord-014597-66vd2mdu	191	10	Fed	Fed	NNP
cord-014597-66vd2mdu	191	11	batch	batch	NN
cord-014597-66vd2mdu	191	12	run	run	NN
cord-014597-66vd2mdu	191	13	(	(	-LRB-
cord-014597-66vd2mdu	191	14	Fig	fig	NN
cord-014597-66vd2mdu	191	15	.	.	.
cord-014597-66vd2mdu	191	16	1a	1a	CD
cord-014597-66vd2mdu	191	17	)	)	-RRB-
cord-014597-66vd2mdu	191	18	.	.	.
cord-014597-66vd2mdu	192	1	Considering	consider	VBG
cord-014597-66vd2mdu	192	2	the	the	DT
cord-014597-66vd2mdu	192	3	number	number	NN
cord-014597-66vd2mdu	192	4	of	of	IN
cord-014597-66vd2mdu	192	5	runs	run	NNS
cord-014597-66vd2mdu	192	6	that	that	WDT
cord-014597-66vd2mdu	192	7	could	could	MD
cord-014597-66vd2mdu	192	8	be	be	VB
cord-014597-66vd2mdu	192	9	executed	execute	VBN
cord-014597-66vd2mdu	192	10	in	in	IN
cord-014597-66vd2mdu	192	11	a	a	DT
cord-014597-66vd2mdu	192	12	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	192	13	facility	facility	NN
cord-014597-66vd2mdu	192	14	within	within	IN
cord-014597-66vd2mdu	192	15	the	the	DT
cord-014597-66vd2mdu	192	16	same	same	JJ
cord-014597-66vd2mdu	192	17	calendar	calendar	NN
cord-014597-66vd2mdu	192	18	days	day	NNS
cord-014597-66vd2mdu	192	19	,	,	,
cord-014597-66vd2mdu	192	20	about	about	RB
cord-014597-66vd2mdu	192	21	1fold	1fold	CD
cord-014597-66vd2mdu	192	22	increase	increase	NN
cord-014597-66vd2mdu	192	23	in	in	IN
cord-014597-66vd2mdu	192	24	product	product	NN
cord-014597-66vd2mdu	192	25	output	output	NN
cord-014597-66vd2mdu	192	26	can	can	MD
cord-014597-66vd2mdu	192	27	be	be	VB
cord-014597-66vd2mdu	192	28	achieved	achieve	VBN
cord-014597-66vd2mdu	192	29	with	with	IN
cord-014597-66vd2mdu	192	30	the	the	DT
cord-014597-66vd2mdu	192	31	perfusion	perfusion	NN
cord-014597-66vd2mdu	192	32	process	process	NN
cord-014597-66vd2mdu	192	33	(	(	-LRB-
cord-014597-66vd2mdu	192	34	Fig	Fig	NNP
cord-014597-66vd2mdu	192	35	.	.	.
cord-014597-66vd2mdu	192	36	1a	1a	CD
cord-014597-66vd2mdu	192	37	)	)	-RRB-
cord-014597-66vd2mdu	192	38	.	.	.
cord-014597-66vd2mdu	193	1	This	this	DT
cord-014597-66vd2mdu	193	2	difference	difference	NN
cord-014597-66vd2mdu	193	3	is	be	VBZ
cord-014597-66vd2mdu	193	4	attributed	attribute	VBN
cord-014597-66vd2mdu	193	5	to	to	IN
cord-014597-66vd2mdu	193	6	higher	high	JJR
cord-014597-66vd2mdu	193	7	IVCC	IVCC	NNP
cord-014597-66vd2mdu	193	8	,	,	,
cord-014597-66vd2mdu	193	9	higher	high	JJR
cord-014597-66vd2mdu	193	10	PCD	PCD	NNP
cord-014597-66vd2mdu	193	11	and	and	CC
cord-014597-66vd2mdu	193	12	longevity	longevity	NN
cord-014597-66vd2mdu	193	13	of	of	IN
cord-014597-66vd2mdu	193	14	cells	cell	NNS
cord-014597-66vd2mdu	193	15	because	because	IN
cord-014597-66vd2mdu	193	16	of	of	IN
cord-014597-66vd2mdu	193	17	decreased	decreased	JJ
cord-014597-66vd2mdu	193	18	level	level	NN
cord-014597-66vd2mdu	193	19	of	of	IN
cord-014597-66vd2mdu	193	20	toxic	toxic	JJ
cord-014597-66vd2mdu	193	21	metabolite	metabolite	JJ
cord-014597-66vd2mdu	193	22	concentrations	concentration	NNS
cord-014597-66vd2mdu	193	23	such	such	JJ
cord-014597-66vd2mdu	193	24	as	as	IN
cord-014597-66vd2mdu	193	25	lactate	lactate	NN
cord-014597-66vd2mdu	193	26	and	and	CC
cord-014597-66vd2mdu	193	27	ammonia	ammonia	NN
cord-014597-66vd2mdu	193	28	.	.	.
cord-014597-66vd2mdu	194	1	Case	case	NN
cord-014597-66vd2mdu	194	2	2	2	CD
cord-014597-66vd2mdu	194	3	:	:	:
cord-014597-66vd2mdu	195	1	Understanding	understand	VBG
cord-014597-66vd2mdu	195	2	product	product	NN
cord-014597-66vd2mdu	195	3	retention	retention	NN
cord-014597-66vd2mdu	195	4	in	in	IN
cord-014597-66vd2mdu	195	5	perfusion	perfusion	NN
cord-014597-66vd2mdu	195	6	process	process	NN
cord-014597-66vd2mdu	196	1	The	the	DT
cord-014597-66vd2mdu	196	2	new	new	JJ
cord-014597-66vd2mdu	196	3	-	-	HYPH
cord-014597-66vd2mdu	196	4	age	age	NN
cord-014597-66vd2mdu	196	5	perfusion	perfusion	NN
cord-014597-66vd2mdu	196	6	processes	process	NNS
cord-014597-66vd2mdu	196	7	utilize	utilize	VBP
cord-014597-66vd2mdu	196	8	hollow	hollow	JJ
cord-014597-66vd2mdu	196	9	fiber	fiber	NN
cord-014597-66vd2mdu	196	10	filters	filter	NNS
cord-014597-66vd2mdu	196	11	.	.	.
cord-014597-66vd2mdu	197	1	This	this	DT
cord-014597-66vd2mdu	197	2	has	have	VBZ
cord-014597-66vd2mdu	197	3	been	be	VBN
cord-014597-66vd2mdu	197	4	observed	observe	VBN
cord-014597-66vd2mdu	197	5	to	to	TO
cord-014597-66vd2mdu	197	6	cause	cause	VB
cord-014597-66vd2mdu	197	7	retention	retention	NN
cord-014597-66vd2mdu	197	8	of	of	IN
cord-014597-66vd2mdu	197	9	product	product	NN
cord-014597-66vd2mdu	197	10	within	within	IN
cord-014597-66vd2mdu	197	11	the	the	DT
cord-014597-66vd2mdu	197	12	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	197	13	especially	especially	RB
cord-014597-66vd2mdu	197	14	towards	towards	IN
cord-014597-66vd2mdu	197	15	the	the	DT
cord-014597-66vd2mdu	197	16	end	end	NN
cord-014597-66vd2mdu	197	17	of	of	IN
cord-014597-66vd2mdu	197	18	the	the	DT
cord-014597-66vd2mdu	197	19	production	production	NN
cord-014597-66vd2mdu	197	20	run	run	NN
cord-014597-66vd2mdu	197	21	.	.	.
cord-014597-66vd2mdu	198	1	Two	two	CD
cord-014597-66vd2mdu	198	2	types	type	NNS
cord-014597-66vd2mdu	198	3	of	of	IN
cord-014597-66vd2mdu	198	4	experiments	experiment	NNS
cord-014597-66vd2mdu	198	5	were	be	VBD
cord-014597-66vd2mdu	198	6	conducted	conduct	VBN
cord-014597-66vd2mdu	198	7	to	to	TO
cord-014597-66vd2mdu	198	8	study	study	VB
cord-014597-66vd2mdu	198	9	the	the	DT
cord-014597-66vd2mdu	198	10	factors	factor	NNS
cord-014597-66vd2mdu	198	11	contributing	contribute	VBG
cord-014597-66vd2mdu	198	12	to	to	IN
cord-014597-66vd2mdu	198	13	product	product	NN
cord-014597-66vd2mdu	198	14	retention	retention	NN
cord-014597-66vd2mdu	198	15	:	:	:
cord-014597-66vd2mdu	199	1	-Spiking	-Spiking	NNP
cord-014597-66vd2mdu	199	2	studies	study	NNS
cord-014597-66vd2mdu	199	3	:	:	:
cord-014597-66vd2mdu	200	1	-Role	-Role	:
cord-014597-66vd2mdu	200	2	of	of	IN
cord-014597-66vd2mdu	200	3	product	product	NN
cord-014597-66vd2mdu	200	4	titer	titer	NN
cord-014597-66vd2mdu	200	5	:	:	:
cord-014597-66vd2mdu	200	6	product	product	NN
cord-014597-66vd2mdu	200	7	was	be	VBD
cord-014597-66vd2mdu	200	8	spiked	spike	VBN
cord-014597-66vd2mdu	200	9	into	into	IN
cord-014597-66vd2mdu	200	10	chemically	chemically	RB
cord-014597-66vd2mdu	200	11	defined	define	VBN
cord-014597-66vd2mdu	200	12	media	medium	NNS
cord-014597-66vd2mdu	200	13	-Role	-Role	:
cord-014597-66vd2mdu	200	14	of	of	IN
cord-014597-66vd2mdu	200	15	different	different	JJ
cord-014597-66vd2mdu	200	16	cell	cell	NN
cord-014597-66vd2mdu	200	17	viability	viability	NN
cord-014597-66vd2mdu	200	18	:	:	:
cord-014597-66vd2mdu	200	19	different	different	JJ
cord-014597-66vd2mdu	200	20	broths	broth	NNS
cord-014597-66vd2mdu	200	21	with	with	IN
cord-014597-66vd2mdu	200	22	varying	vary	VBG
cord-014597-66vd2mdu	200	23	viability	viability	NN
cord-014597-66vd2mdu	200	24	spiked	spike	VBN
cord-014597-66vd2mdu	200	25	with	with	IN
cord-014597-66vd2mdu	200	26	same	same	JJ
cord-014597-66vd2mdu	200	27	product	product	NN
cord-014597-66vd2mdu	200	28	titer	titer	NN
cord-014597-66vd2mdu	200	29	-Evaluation	-evaluation	NN
cord-014597-66vd2mdu	200	30	of	of	IN
cord-014597-66vd2mdu	200	31	different	different	JJ
cord-014597-66vd2mdu	200	32	hollow	hollow	JJ
cord-014597-66vd2mdu	200	33	fiber	fiber	NN
cord-014597-66vd2mdu	200	34	membrane	membrane	NN
cord-014597-66vd2mdu	200	35	(	(	-LRB-
cord-014597-66vd2mdu	200	36	M1	M1	NNP
cord-014597-66vd2mdu	200	37	,	,	,
cord-014597-66vd2mdu	200	38	M2	M2	NNP
cord-014597-66vd2mdu	200	39	and	and	CC
cord-014597-66vd2mdu	200	40	M3	M3	NNP
cord-014597-66vd2mdu	200	41	)	)	-RRB-
cord-014597-66vd2mdu	200	42	on	on	IN
cord-014597-66vd2mdu	200	43	product	product	NN
cord-014597-66vd2mdu	200	44	retention	retention	NN
cord-014597-66vd2mdu	200	45	.	.	.
cord-014597-66vd2mdu	201	1	From	from	IN
cord-014597-66vd2mdu	201	2	spiking	spike	VBG
cord-014597-66vd2mdu	201	3	studies	study	NNS
cord-014597-66vd2mdu	201	4	,	,	,
cord-014597-66vd2mdu	201	5	it	-PRON-	PRP
cord-014597-66vd2mdu	201	6	was	be	VBD
cord-014597-66vd2mdu	201	7	evident	evident	JJ
cord-014597-66vd2mdu	201	8	that	that	IN
cord-014597-66vd2mdu	201	9	cell	cell	NN
cord-014597-66vd2mdu	201	10	debris	debris	NN
cord-014597-66vd2mdu	201	11	and	and	CC
cord-014597-66vd2mdu	201	12	poor	poor	JJ
cord-014597-66vd2mdu	201	13	quality	quality	NN
cord-014597-66vd2mdu	201	14	cell	cell	NN
cord-014597-66vd2mdu	201	15	broth	broth	NN
cord-014597-66vd2mdu	201	16	(	(	-LRB-
cord-014597-66vd2mdu	201	17	lower	low	JJR
cord-014597-66vd2mdu	201	18	viability	viability	NN
cord-014597-66vd2mdu	201	19	)	)	-RRB-
cord-014597-66vd2mdu	201	20	were	be	VBD
cord-014597-66vd2mdu	201	21	the	the	DT
cord-014597-66vd2mdu	201	22	major	major	JJ
cord-014597-66vd2mdu	201	23	factors	factor	NNS
cord-014597-66vd2mdu	201	24	contributing	contribute	VBG
cord-014597-66vd2mdu	201	25	to	to	IN
cord-014597-66vd2mdu	201	26	product	product	NN
cord-014597-66vd2mdu	201	27	retention	retention	NN
cord-014597-66vd2mdu	201	28	(	(	-LRB-
cord-014597-66vd2mdu	201	29	Fig	Fig	NNP
cord-014597-66vd2mdu	201	30	.	.	.
cord-014597-66vd2mdu	201	31	1c	1c	CD
cord-014597-66vd2mdu	201	32	)	)	-RRB-
cord-014597-66vd2mdu	201	33	.	.	.
cord-014597-66vd2mdu	202	1	From	from	IN
cord-014597-66vd2mdu	202	2	the	the	DT
cord-014597-66vd2mdu	202	3	different	different	JJ
cord-014597-66vd2mdu	202	4	membranes	membrane	NNS
cord-014597-66vd2mdu	202	5	experiments	experiment	NNS
cord-014597-66vd2mdu	202	6	,	,	,
cord-014597-66vd2mdu	202	7	it	-PRON-	PRP
cord-014597-66vd2mdu	202	8	was	be	VBD
cord-014597-66vd2mdu	202	9	identified	identify	VBN
cord-014597-66vd2mdu	202	10	that	that	IN
cord-014597-66vd2mdu	202	11	at	at	IN
cord-014597-66vd2mdu	202	12	Pilot	pilot	NN
cord-014597-66vd2mdu	202	13	scale	scale	NN
cord-014597-66vd2mdu	202	14	,	,	,
cord-014597-66vd2mdu	202	15	M1	M1	NNP
cord-014597-66vd2mdu	202	16	showed	show	VBD
cord-014597-66vd2mdu	202	17	much	much	RB
cord-014597-66vd2mdu	202	18	higher	high	JJR
cord-014597-66vd2mdu	202	19	retention	retention	NN
cord-014597-66vd2mdu	202	20	from	from	IN
cord-014597-66vd2mdu	202	21	the	the	DT
cord-014597-66vd2mdu	202	22	first	first	JJ
cord-014597-66vd2mdu	202	23	perfusion	perfusion	NN
cord-014597-66vd2mdu	202	24	cycle	cycle	NN
cord-014597-66vd2mdu	202	25	itself	-PRON-	PRP
cord-014597-66vd2mdu	203	1	and	and	CC
cord-014597-66vd2mdu	203	2	it	-PRON-	PRP
cord-014597-66vd2mdu	203	3	increased	increase	VBD
cord-014597-66vd2mdu	203	4	to	to	IN
cord-014597-66vd2mdu	203	5	more	more	JJR
cord-014597-66vd2mdu	203	6	than	than	IN
cord-014597-66vd2mdu	203	7	75	75	CD
cord-014597-66vd2mdu	203	8	%	%	NN
cord-014597-66vd2mdu	203	9	towards	towards	IN
cord-014597-66vd2mdu	203	10	the	the	DT
cord-014597-66vd2mdu	203	11	end	end	NN
cord-014597-66vd2mdu	203	12	of	of	IN
cord-014597-66vd2mdu	203	13	the	the	DT
cord-014597-66vd2mdu	203	14	batch	batch	NN
cord-014597-66vd2mdu	203	15	.	.	.
cord-014597-66vd2mdu	204	1	However	however	RB
cord-014597-66vd2mdu	204	2	,	,	,
cord-014597-66vd2mdu	204	3	with	with	IN
cord-014597-66vd2mdu	204	4	M2	M2	NNP
cord-014597-66vd2mdu	204	5	membrane	membrane	NN
cord-014597-66vd2mdu	204	6	,	,	,
cord-014597-66vd2mdu	204	7	product	product	NN
cord-014597-66vd2mdu	204	8	retention	retention	NN
cord-014597-66vd2mdu	204	9	started	start	VBD
cord-014597-66vd2mdu	204	10	only	only	RB
cord-014597-66vd2mdu	204	11	late	late	RB
cord-014597-66vd2mdu	204	12	(	(	-LRB-
cord-014597-66vd2mdu	204	13	after	after	IN
cord-014597-66vd2mdu	204	14	50	50	CD
cord-014597-66vd2mdu	204	15	%	%	NN
cord-014597-66vd2mdu	204	16	of	of	IN
cord-014597-66vd2mdu	204	17	batch	batch	NN
cord-014597-66vd2mdu	204	18	duration	duration	NN
cord-014597-66vd2mdu	204	19	)	)	-RRB-
cord-014597-66vd2mdu	204	20	and	and	CC
cord-014597-66vd2mdu	204	21	it	-PRON-	PRP
cord-014597-66vd2mdu	204	22	remained	remain	VBD
cord-014597-66vd2mdu	204	23	low	low	JJ
cord-014597-66vd2mdu	204	24	(	(	-LRB-
cord-014597-66vd2mdu	204	25	~20	~20	NFP
cord-014597-66vd2mdu	204	26	-	-	:
cord-014597-66vd2mdu	204	27	40	40	CD
cord-014597-66vd2mdu	204	28	%	%	NN
cord-014597-66vd2mdu	204	29	)	)	-RRB-
cord-014597-66vd2mdu	204	30	.	.	.
cord-014597-66vd2mdu	205	1	On	on	IN
cord-014597-66vd2mdu	205	2	the	the	DT
cord-014597-66vd2mdu	205	3	contrary	contrary	NN
cord-014597-66vd2mdu	205	4	,	,	,
cord-014597-66vd2mdu	205	5	this	this	DT
cord-014597-66vd2mdu	205	6	difference	difference	NN
cord-014597-66vd2mdu	205	7	was	be	VBD
cord-014597-66vd2mdu	205	8	not	not	RB
cord-014597-66vd2mdu	205	9	observed	observe	VBN
cord-014597-66vd2mdu	205	10	at	at	IN
cord-014597-66vd2mdu	205	11	1L	1l	CD
cord-014597-66vd2mdu	205	12	scale	scale	NN
cord-014597-66vd2mdu	205	13	due	due	IN
cord-014597-66vd2mdu	205	14	to	to	IN
cord-014597-66vd2mdu	205	15	the	the	DT
cord-014597-66vd2mdu	205	16	usage	usage	NN
cord-014597-66vd2mdu	205	17	of	of	IN
cord-014597-66vd2mdu	205	18	membranes	membrane	NNS
cord-014597-66vd2mdu	205	19	with	with	IN
cord-014597-66vd2mdu	205	20	larger	large	JJR
cord-014597-66vd2mdu	205	21	filter	filter	NN
cord-014597-66vd2mdu	205	22	area	area	NN
cord-014597-66vd2mdu	205	23	(	(	-LRB-
cord-014597-66vd2mdu	205	24	2	2	CD
cord-014597-66vd2mdu	205	25	-	-	SYM
cord-014597-66vd2mdu	205	26	3	3	CD
cord-014597-66vd2mdu	205	27	folds	fold	NNS
cord-014597-66vd2mdu	205	28	higher	higher	RBR
cord-014597-66vd2mdu	205	29	compared	compare	VBN
cord-014597-66vd2mdu	205	30	to	to	IN
cord-014597-66vd2mdu	205	31	pilot	pilot	NN
cord-014597-66vd2mdu	205	32	scale	scale	NN
cord-014597-66vd2mdu	205	33	)	)	-RRB-
cord-014597-66vd2mdu	205	34	.	.	.
cord-014597-66vd2mdu	206	1	When	when	WRB
cord-014597-66vd2mdu	206	2	the	the	DT
cord-014597-66vd2mdu	206	3	filter	filter	NN
cord-014597-66vd2mdu	206	4	area	area	NN
cord-014597-66vd2mdu	206	5	per	per	IN
cord-014597-66vd2mdu	206	6	unit	unit	NN
cord-014597-66vd2mdu	206	7	volume	volume	NN
cord-014597-66vd2mdu	206	8	of	of	IN
cord-014597-66vd2mdu	206	9	perfusate	perfusate	NN
cord-014597-66vd2mdu	206	10	was	be	VBD
cord-014597-66vd2mdu	206	11	decreased	decrease	VBN
cord-014597-66vd2mdu	206	12	by	by	IN
cord-014597-66vd2mdu	206	13	half	half	NN
cord-014597-66vd2mdu	206	14	(	(	-LRB-
cord-014597-66vd2mdu	206	15	M2_Batch	M2_Batch	NNP
cord-014597-66vd2mdu	206	16	4	4	CD
cord-014597-66vd2mdu	206	17	)	)	-RRB-
cord-014597-66vd2mdu	206	18	for	for	IN
cord-014597-66vd2mdu	206	19	the	the	DT
cord-014597-66vd2mdu	206	20	pilot	pilot	NN
cord-014597-66vd2mdu	206	21	scale	scale	NN
cord-014597-66vd2mdu	206	22	,	,	,
cord-014597-66vd2mdu	206	23	even	even	RB
cord-014597-66vd2mdu	206	24	M2	M2	NNP
cord-014597-66vd2mdu	206	25	showed	show	VBD
cord-014597-66vd2mdu	206	26	retention	retention	NN
cord-014597-66vd2mdu	206	27	profile	profile	NN
cord-014597-66vd2mdu	206	28	similar	similar	JJ
cord-014597-66vd2mdu	206	29	to	to	IN
cord-014597-66vd2mdu	206	30	M1	M1	NNP
cord-014597-66vd2mdu	206	31	(	(	-LRB-
cord-014597-66vd2mdu	206	32	Fig	Fig	NNP
cord-014597-66vd2mdu	206	33	.	.	.
cord-014597-66vd2mdu	206	34	1d	1d	CD
cord-014597-66vd2mdu	206	35	)	)	-RRB-
cord-014597-66vd2mdu	206	36	.	.	.
cord-014597-66vd2mdu	207	1	We	-PRON-	PRP
cord-014597-66vd2mdu	207	2	presented	present	VBD
cord-014597-66vd2mdu	207	3	data	datum	NNS
cord-014597-66vd2mdu	207	4	to	to	TO
cord-014597-66vd2mdu	207	5	show	show	VB
cord-014597-66vd2mdu	207	6	that	that	IN
cord-014597-66vd2mdu	207	7	perfusion	perfusion	NN
cord-014597-66vd2mdu	207	8	process	process	NN
cord-014597-66vd2mdu	207	9	has	have	VBZ
cord-014597-66vd2mdu	207	10	5-fold	5-fold	CD
cord-014597-66vd2mdu	207	11	increase	increase	NN
cord-014597-66vd2mdu	207	12	in	in	IN
cord-014597-66vd2mdu	207	13	product	product	NN
cord-014597-66vd2mdu	207	14	yield	yield	NN
cord-014597-66vd2mdu	207	15	on	on	IN
cord-014597-66vd2mdu	207	16	a	a	DT
cord-014597-66vd2mdu	207	17	per	per	IN
cord-014597-66vd2mdu	207	18	-	-	HYPH
cord-014597-66vd2mdu	207	19	batch	batch	NN
cord-014597-66vd2mdu	207	20	basis	basis	NN
cord-014597-66vd2mdu	207	21	and	and	CC
cord-014597-66vd2mdu	207	22	a	a	DT
cord-014597-66vd2mdu	207	23	3-fold	3-fold	CD
cord-014597-66vd2mdu	207	24	increase	increase	NN
cord-014597-66vd2mdu	207	25	when	when	WRB
cord-014597-66vd2mdu	207	26	facility	facility	NN
cord-014597-66vd2mdu	207	27	throughput	throughput	NN
cord-014597-66vd2mdu	207	28	is	be	VBZ
cord-014597-66vd2mdu	207	29	considered	consider	VBN
cord-014597-66vd2mdu	207	30	.	.	.
cord-014597-66vd2mdu	208	1	Product	product	NN
cord-014597-66vd2mdu	208	2	retention	retention	NN
cord-014597-66vd2mdu	208	3	is	be	VBZ
cord-014597-66vd2mdu	208	4	a	a	DT
cord-014597-66vd2mdu	208	5	technical	technical	JJ
cord-014597-66vd2mdu	208	6	challenge	challenge	NN
cord-014597-66vd2mdu	208	7	that	that	WDT
cord-014597-66vd2mdu	208	8	requires	require	VBZ
cord-014597-66vd2mdu	208	9	optimization	optimization	NN
cord-014597-66vd2mdu	208	10	(	(	-LRB-
cord-014597-66vd2mdu	208	11	perfusion	perfusion	NN
cord-014597-66vd2mdu	208	12	rates	rate	NNS
cord-014597-66vd2mdu	208	13	and	and	CC
cord-014597-66vd2mdu	208	14	filter	filter	NN
cord-014597-66vd2mdu	208	15	membrane	membrane	NN
cord-014597-66vd2mdu	208	16	types	type	NNS
cord-014597-66vd2mdu	208	17	)	)	-RRB-
cord-014597-66vd2mdu	208	18	.	.	.
cord-014597-66vd2mdu	209	1	We	-PRON-	PRP
cord-014597-66vd2mdu	209	2	believe	believe	VBP
cord-014597-66vd2mdu	209	3	it	-PRON-	PRP
cord-014597-66vd2mdu	209	4	is	be	VBZ
cord-014597-66vd2mdu	209	5	imperative	imperative	JJ
cord-014597-66vd2mdu	209	6	that	that	IN
cord-014597-66vd2mdu	209	7	labs	lab	NNS
cord-014597-66vd2mdu	209	8	that	that	WDT
cord-014597-66vd2mdu	209	9	develop	develop	VBP
cord-014597-66vd2mdu	209	10	processes	process	NNS
cord-014597-66vd2mdu	209	11	for	for	IN
cord-014597-66vd2mdu	209	12	biologics	biologic	NNS
cord-014597-66vd2mdu	209	13	can	can	MD
cord-014597-66vd2mdu	209	14	now	now	RB
cord-014597-66vd2mdu	209	15	consider	consider	VB
cord-014597-66vd2mdu	209	16	both	both	DT
cord-014597-66vd2mdu	209	17	perfusion	perfusion	NN
cord-014597-66vd2mdu	209	18	and	and	CC
cord-014597-66vd2mdu	209	19	fedbatch	fedbatch	NN
cord-014597-66vd2mdu	209	20	based	base	VBN
cord-014597-66vd2mdu	209	21	processes	process	NNS
cord-014597-66vd2mdu	209	22	as	as	IN
cord-014597-66vd2mdu	209	23	both	both	PDT
cord-014597-66vd2mdu	209	24	these	these	DT
cord-014597-66vd2mdu	209	25	technologies	technology	NNS
cord-014597-66vd2mdu	209	26	can	can	MD
cord-014597-66vd2mdu	209	27	now	now	RB
cord-014597-66vd2mdu	209	28	closely	closely	RB
cord-014597-66vd2mdu	209	29	compete	compete	VB
cord-014597-66vd2mdu	209	30	with	with	IN
cord-014597-66vd2mdu	209	31	each	each	DT
cord-014597-66vd2mdu	209	32	other	other	JJ
cord-014597-66vd2mdu	209	33	.	.	.
cord-014597-66vd2mdu	210	1	The	the	DT
cord-014597-66vd2mdu	210	2	choice	choice	NN
cord-014597-66vd2mdu	210	3	of	of	IN
cord-014597-66vd2mdu	210	4	the	the	DT
cord-014597-66vd2mdu	210	5	process	process	NN
cord-014597-66vd2mdu	210	6	format	format	NN
cord-014597-66vd2mdu	210	7	going	go	VBG
cord-014597-66vd2mdu	210	8	forward	forward	RB
cord-014597-66vd2mdu	210	9	should	should	MD
cord-014597-66vd2mdu	210	10	now	now	RB
cord-014597-66vd2mdu	210	11	solely	solely	RB
cord-014597-66vd2mdu	210	12	be	be	VB
cord-014597-66vd2mdu	210	13	dependent	dependent	JJ
cord-014597-66vd2mdu	210	14	on	on	IN
cord-014597-66vd2mdu	210	15	the	the	DT
cord-014597-66vd2mdu	210	16	requirement	requirement	NN
cord-014597-66vd2mdu	210	17	for	for	IN
cord-014597-66vd2mdu	210	18	the	the	DT
cord-014597-66vd2mdu	210	19	biologic	biologic	NN
cord-014597-66vd2mdu	210	20	rather	rather	RB
cord-014597-66vd2mdu	210	21	than	than	IN
cord-014597-66vd2mdu	210	22	the	the	DT
cord-014597-66vd2mdu	210	23	earlier	early	JJR
cord-014597-66vd2mdu	210	24	perception	perception	NN
cord-014597-66vd2mdu	210	25	that	that	IN
cord-014597-66vd2mdu	210	26	fed	fed	NN
cord-014597-66vd2mdu	210	27	-	-	HYPH
cord-014597-66vd2mdu	210	28	batch	batch	NN
cord-014597-66vd2mdu	210	29	is	be	VBZ
cord-014597-66vd2mdu	210	30	the	the	DT
cord-014597-66vd2mdu	210	31	preferred	preferred	JJ
cord-014597-66vd2mdu	210	32	choice	choice	NN
cord-014597-66vd2mdu	210	33	because	because	IN
cord-014597-66vd2mdu	210	34	of	of	IN
cord-014597-66vd2mdu	210	35	its	-PRON-	PRP$
cord-014597-66vd2mdu	210	36	simplicity	simplicity	NN
cord-014597-66vd2mdu	210	37	.	.	.
cord-014597-66vd2mdu	211	1	Background	background	NN
cord-014597-66vd2mdu	211	2	Chinese	chinese	JJ
cord-014597-66vd2mdu	211	3	hamster	hamster	NN
cord-014597-66vd2mdu	211	4	ovary	ovary	NN
cord-014597-66vd2mdu	212	1	(	(	-LRB-
cord-014597-66vd2mdu	212	2	CHO	CHO	NNP
cord-014597-66vd2mdu	212	3	)	)	-RRB-
cord-014597-66vd2mdu	212	4	cell	cell	NN
cord-014597-66vd2mdu	212	5	culture	culture	NN
cord-014597-66vd2mdu	212	6	has	have	VBZ
cord-014597-66vd2mdu	212	7	been	be	VBN
cord-014597-66vd2mdu	212	8	widely	widely	RB
cord-014597-66vd2mdu	212	9	used	use	VBN
cord-014597-66vd2mdu	212	10	for	for	IN
cord-014597-66vd2mdu	212	11	production	production	NN
cord-014597-66vd2mdu	212	12	of	of	IN
cord-014597-66vd2mdu	212	13	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	212	14	antibodies	antibody	NNS
cord-014597-66vd2mdu	212	15	in	in	IN
cord-014597-66vd2mdu	212	16	the	the	DT
cord-014597-66vd2mdu	212	17	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	212	18	industry	industry	NN
cord-014597-66vd2mdu	212	19	.	.	.
cord-014597-66vd2mdu	213	1	Previous	previous	JJ
cord-014597-66vd2mdu	213	2	studies	study	NNS
cord-014597-66vd2mdu	213	3	have	have	VBP
cord-014597-66vd2mdu	213	4	shown	show	VBN
cord-014597-66vd2mdu	213	5	that	that	IN
cord-014597-66vd2mdu	213	6	the	the	DT
cord-014597-66vd2mdu	213	7	cell	cell	NN
cord-014597-66vd2mdu	213	8	specific	specific	JJ
cord-014597-66vd2mdu	213	9	productivity	productivity	NN
cord-014597-66vd2mdu	213	10	in	in	IN
cord-014597-66vd2mdu	213	11	CHO	CHO	NNP
cord-014597-66vd2mdu	213	12	cells	cell	NNS
cord-014597-66vd2mdu	213	13	can	can	MD
cord-014597-66vd2mdu	213	14	be	be	VB
cord-014597-66vd2mdu	213	15	increased	increase	VBN
cord-014597-66vd2mdu	213	16	by	by	IN
cord-014597-66vd2mdu	213	17	glucose	glucose	NN
cord-014597-66vd2mdu	213	18	limitation	limitation	NN
cord-014597-66vd2mdu	213	19	[	[	-LRB-
cord-014597-66vd2mdu	213	20	1	1	CD
cord-014597-66vd2mdu	213	21	]	]	-RRB-
cord-014597-66vd2mdu	213	22	.	.	.
cord-014597-66vd2mdu	214	1	Introducing	introduce	VBG
cord-014597-66vd2mdu	214	2	a	a	DT
cord-014597-66vd2mdu	214	3	productivity	productivity	NN
cord-014597-66vd2mdu	214	4	enhancing	enhance	VBG
cord-014597-66vd2mdu	214	5	effect	effect	NN
cord-014597-66vd2mdu	214	6	it	-PRON-	PRP
cord-014597-66vd2mdu	214	7	is	be	VBZ
cord-014597-66vd2mdu	214	8	possible	possible	JJ
cord-014597-66vd2mdu	214	9	that	that	IN
cord-014597-66vd2mdu	214	10	this	this	DT
cord-014597-66vd2mdu	214	11	also	also	RB
cord-014597-66vd2mdu	214	12	affects	affect	VBZ
cord-014597-66vd2mdu	214	13	the	the	DT
cord-014597-66vd2mdu	214	14	quality	quality	NN
cord-014597-66vd2mdu	214	15	of	of	IN
cord-014597-66vd2mdu	214	16	the	the	DT
cord-014597-66vd2mdu	214	17	product	product	NN
cord-014597-66vd2mdu	214	18	such	such	JJ
cord-014597-66vd2mdu	214	19	as	as	IN
cord-014597-66vd2mdu	214	20	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	214	21	or	or	CC
cord-014597-66vd2mdu	214	22	other	other	JJ
cord-014597-66vd2mdu	214	23	posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	214	24	modifications	modification	NNS
cord-014597-66vd2mdu	214	25	.	.	.
cord-014597-66vd2mdu	215	1	In	in	IN
cord-014597-66vd2mdu	215	2	this	this	DT
cord-014597-66vd2mdu	215	3	work	work	NN
cord-014597-66vd2mdu	215	4	,	,	,
cord-014597-66vd2mdu	215	5	we	-PRON-	PRP
cord-014597-66vd2mdu	215	6	are	be	VBP
cord-014597-66vd2mdu	215	7	focusing	focus	VBG
cord-014597-66vd2mdu	215	8	on	on	IN
cord-014597-66vd2mdu	215	9	the	the	DT
cord-014597-66vd2mdu	215	10	impact	impact	NN
cord-014597-66vd2mdu	215	11	of	of	IN
cord-014597-66vd2mdu	215	12	glucose	glucose	NN
cord-014597-66vd2mdu	215	13	limitation	limitation	NN
cord-014597-66vd2mdu	215	14	and	and	CC
cord-014597-66vd2mdu	215	15	increased	increase	VBN
cord-014597-66vd2mdu	215	16	productivity	productivity	NN
cord-014597-66vd2mdu	215	17	on	on	IN
cord-014597-66vd2mdu	215	18	the	the	DT
cord-014597-66vd2mdu	215	19	product	product	NN
cord-014597-66vd2mdu	215	20	quality	quality	NN
cord-014597-66vd2mdu	215	21	of	of	IN
cord-014597-66vd2mdu	215	22	a	a	DT
cord-014597-66vd2mdu	215	23	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	215	24	antibody	antibody	NN
cord-014597-66vd2mdu	215	25	produced	produce	VBN
cord-014597-66vd2mdu	215	26	in	in	IN
cord-014597-66vd2mdu	215	27	a	a	DT
cord-014597-66vd2mdu	215	28	fed	feed	VBN
cord-014597-66vd2mdu	215	29	-	-	HYPH
cord-014597-66vd2mdu	215	30	batch	batch	NN
cord-014597-66vd2mdu	215	31	cultivation	cultivation	NN
cord-014597-66vd2mdu	215	32	of	of	IN
cord-014597-66vd2mdu	215	33	CHO	CHO	NNP
cord-014597-66vd2mdu	215	34	cells	cell	NNS
cord-014597-66vd2mdu	215	35	.	.	.
cord-014597-66vd2mdu	216	1	Materials	material	NNS
cord-014597-66vd2mdu	216	2	and	and	CC
cord-014597-66vd2mdu	216	3	methods	method	NNS
cord-014597-66vd2mdu	216	4	CHO	CHO	NNP
cord-014597-66vd2mdu	216	5	cells	cell	NNS
cord-014597-66vd2mdu	216	6	were	be	VBD
cord-014597-66vd2mdu	216	7	cultivated	cultivate	VBN
cord-014597-66vd2mdu	216	8	both	both	DT
cord-014597-66vd2mdu	216	9	under	under	IN
cord-014597-66vd2mdu	216	10	limiting	limit	VBG
cord-014597-66vd2mdu	216	11	and	and	CC
cord-014597-66vd2mdu	216	12	non	non	JJ
cord-014597-66vd2mdu	216	13	-	-	JJ
cord-014597-66vd2mdu	216	14	limiting	limit	VBG
cord-014597-66vd2mdu	216	15	nutrient	nutrient	NN
cord-014597-66vd2mdu	216	16	conditions	condition	NNS
cord-014597-66vd2mdu	216	17	in	in	IN
cord-014597-66vd2mdu	216	18	fed	fed	NNP
cord-014597-66vd2mdu	216	19	-	-	HYPH
cord-014597-66vd2mdu	216	20	batch	batch	NN
cord-014597-66vd2mdu	216	21	.	.	.
cord-014597-66vd2mdu	217	1	For	for	IN
cord-014597-66vd2mdu	217	2	fed	fed	JJ
cord-014597-66vd2mdu	217	3	-	-	HYPH
cord-014597-66vd2mdu	217	4	batch	batch	NN
cord-014597-66vd2mdu	217	5	cultivation	cultivation	NN
cord-014597-66vd2mdu	217	6	the	the	DT
cord-014597-66vd2mdu	217	7	reduced	reduce	VBN
cord-014597-66vd2mdu	217	8	range	range	NN
cord-014597-66vd2mdu	217	9	for	for	IN
cord-014597-66vd2mdu	217	10	glucose	glucose	NN
cord-014597-66vd2mdu	217	11	concentration	concentration	NN
cord-014597-66vd2mdu	217	12	was	be	VBD
cord-014597-66vd2mdu	217	13	chosen	choose	VBN
cord-014597-66vd2mdu	217	14	between	between	IN
cord-014597-66vd2mdu	217	15	0.2	0.2	CD
cord-014597-66vd2mdu	217	16	and	and	CC
cord-014597-66vd2mdu	217	17	0.5	0.5	CD
cord-014597-66vd2mdu	217	18	g/	g/	NNP
cord-014597-66vd2mdu	217	19	L.	L.	NNP
cord-014597-66vd2mdu	217	20	Reference	Reference	NNP
cord-014597-66vd2mdu	217	21	cultivation	cultivation	NN
cord-014597-66vd2mdu	217	22	was	be	VBD
cord-014597-66vd2mdu	217	23	performed	perform	VBN
cord-014597-66vd2mdu	217	24	between	between	IN
cord-014597-66vd2mdu	217	25	1.5	1.5	CD
cord-014597-66vd2mdu	217	26	and	and	CC
cord-014597-66vd2mdu	217	27	3.0	3.0	CD
cord-014597-66vd2mdu	217	28	g	g	NN
cord-014597-66vd2mdu	217	29	/	/	SYM
cord-014597-66vd2mdu	217	30	L.	L.	NNP
cord-014597-66vd2mdu	218	1	Both	both	DT
cord-014597-66vd2mdu	218	2	cultures	culture	NNS
cord-014597-66vd2mdu	218	3	were	be	VBD
cord-014597-66vd2mdu	218	4	fed	feed	VBN
cord-014597-66vd2mdu	218	5	with	with	IN
cord-014597-66vd2mdu	218	6	similar	similar	JJ
cord-014597-66vd2mdu	218	7	volumes	volume	NNS
cord-014597-66vd2mdu	218	8	of	of	IN
cord-014597-66vd2mdu	218	9	a	a	DT
cord-014597-66vd2mdu	218	10	complex	complex	JJ
cord-014597-66vd2mdu	218	11	nutrient	nutrient	NN
cord-014597-66vd2mdu	218	12	supplement	supplement	NN
cord-014597-66vd2mdu	218	13	.	.	.
cord-014597-66vd2mdu	219	1	All	all	DT
cord-014597-66vd2mdu	219	2	cultivations	cultivation	NNS
cord-014597-66vd2mdu	219	3	were	be	VBD
cord-014597-66vd2mdu	219	4	performed	perform	VBN
cord-014597-66vd2mdu	219	5	in	in	IN
cord-014597-66vd2mdu	219	6	chemically	chemically	RB
cord-014597-66vd2mdu	219	7	-	-	HYPH
cord-014597-66vd2mdu	219	8	defined	define	VBN
cord-014597-66vd2mdu	219	9	,	,	,
cord-014597-66vd2mdu	219	10	animalcomponent	animalcomponent	JJ
cord-014597-66vd2mdu	219	11	free	free	JJ
cord-014597-66vd2mdu	219	12	CHO	CHO	NNP
cord-014597-66vd2mdu	219	13	growth	growth	NN
cord-014597-66vd2mdu	219	14	media	medium	NNS
cord-014597-66vd2mdu	219	15	(	(	-LRB-
cord-014597-66vd2mdu	219	16	Xell	Xell	NNP
cord-014597-66vd2mdu	219	17	AG	AG	NNP
cord-014597-66vd2mdu	219	18	)	)	-RRB-
cord-014597-66vd2mdu	219	19	.	.	.
cord-014597-66vd2mdu	220	1	Viable	viable	JJ
cord-014597-66vd2mdu	220	2	cell	cell	NN
cord-014597-66vd2mdu	220	3	density	density	NN
cord-014597-66vd2mdu	220	4	and	and	CC
cord-014597-66vd2mdu	220	5	viability	viability	NN
cord-014597-66vd2mdu	220	6	were	be	VBD
cord-014597-66vd2mdu	220	7	determined	determine	VBN
cord-014597-66vd2mdu	220	8	using	use	VBG
cord-014597-66vd2mdu	220	9	the	the	DT
cord-014597-66vd2mdu	220	10	automated	automate	VBN
cord-014597-66vd2mdu	220	11	cell	cell	NN
cord-014597-66vd2mdu	220	12	counting	counting	NN
cord-014597-66vd2mdu	220	13	system	system	NN
cord-014597-66vd2mdu	220	14	CEDEX	CEDEX	NNP
cord-014597-66vd2mdu	220	15	(	(	-LRB-
cord-014597-66vd2mdu	220	16	Roche	Roche	NNP
cord-014597-66vd2mdu	220	17	Diagnostics	Diagnostics	NNP
cord-014597-66vd2mdu	220	18	)	)	-RRB-
cord-014597-66vd2mdu	220	19	,	,	,
cord-014597-66vd2mdu	220	20	glucose	glucose	NN
cord-014597-66vd2mdu	220	21	and	and	CC
cord-014597-66vd2mdu	220	22	lactate	lactate	NN
cord-014597-66vd2mdu	220	23	concentrations	concentration	NNS
cord-014597-66vd2mdu	220	24	were	be	VBD
cord-014597-66vd2mdu	220	25	detected	detect	VBN
cord-014597-66vd2mdu	220	26	via	via	IN
cord-014597-66vd2mdu	220	27	YSI	YSI	NNP
cord-014597-66vd2mdu	220	28	(	(	-LRB-
cord-014597-66vd2mdu	220	29	YSI	YSI	NNP
cord-014597-66vd2mdu	220	30	life	life	NN
cord-014597-66vd2mdu	220	31	sciences	science	NNS
cord-014597-66vd2mdu	220	32	)	)	-RRB-
cord-014597-66vd2mdu	220	33	.	.	.
cord-014597-66vd2mdu	221	1	Amino	amino	NN
cord-014597-66vd2mdu	221	2	acid	acid	NN
cord-014597-66vd2mdu	221	3	were	be	VBD
cord-014597-66vd2mdu	221	4	quantified	quantify	VBN
cord-014597-66vd2mdu	221	5	using	use	VBG
cord-014597-66vd2mdu	221	6	HPLC	HPLC	NNP
cord-014597-66vd2mdu	221	7	-	-	HYPH
cord-014597-66vd2mdu	221	8	FLD	FLD	NNP
cord-014597-66vd2mdu	221	9	,	,	,
cord-014597-66vd2mdu	221	10	vitamins	vitamin	NNS
cord-014597-66vd2mdu	221	11	were	be	VBD
cord-014597-66vd2mdu	221	12	quantified	quantify	VBN
cord-014597-66vd2mdu	221	13	using	use	VBG
cord-014597-66vd2mdu	221	14	reversed	reversed	JJ
cord-014597-66vd2mdu	221	15	phase	phase	NN
cord-014597-66vd2mdu	221	16	chromatography	chromatography	NN
cord-014597-66vd2mdu	221	17	coupled	couple	VBN
cord-014597-66vd2mdu	221	18	to	to	IN
cord-014597-66vd2mdu	221	19	a	a	DT
cord-014597-66vd2mdu	221	20	triple	triple	JJ
cord-014597-66vd2mdu	221	21	quadrupol	quadrupol	NN
cord-014597-66vd2mdu	221	22	mass	mass	NN
cord-014597-66vd2mdu	221	23	spectrometer	spectrometer	NN
cord-014597-66vd2mdu	221	24	(	(	-LRB-
cord-014597-66vd2mdu	221	25	Varian	Varian	NNP
cord-014597-66vd2mdu	221	26	320	320	CD
cord-014597-66vd2mdu	221	27	,	,	,
cord-014597-66vd2mdu	221	28	selected	select	VBN
cord-014597-66vd2mdu	221	29	reaction	reaction	NN
cord-014597-66vd2mdu	221	30	monitoring	monitoring	NN
cord-014597-66vd2mdu	221	31	)	)	-RRB-
cord-014597-66vd2mdu	221	32	.	.	.
cord-014597-66vd2mdu	222	1	Amounts	amount	NNS
cord-014597-66vd2mdu	222	2	of	of	IN
cord-014597-66vd2mdu	222	3	IgG1	IgG1	NNP
cord-014597-66vd2mdu	222	4	were	be	VBD
cord-014597-66vd2mdu	222	5	quantified	quantify	VBN
cord-014597-66vd2mdu	222	6	via	via	IN
cord-014597-66vd2mdu	222	7	Protein	Protein	NNP
cord-014597-66vd2mdu	222	8	A	a	DT
cord-014597-66vd2mdu	222	9	HPLC	HPLC	NNP
cord-014597-66vd2mdu	222	10	,	,	,
cord-014597-66vd2mdu	222	11	mAb	mAb	NNS
cord-014597-66vd2mdu	222	12	purified	purify	VBN
cord-014597-66vd2mdu	222	13	from	from	IN
cord-014597-66vd2mdu	222	14	another	another	DT
cord-014597-66vd2mdu	222	15	CHO	CHO	NNP
cord-014597-66vd2mdu	222	16	cell	cell	NN
cord-014597-66vd2mdu	222	17	clone	clone	NN
cord-014597-66vd2mdu	222	18	was	be	VBD
cord-014597-66vd2mdu	222	19	used	use	VBN
cord-014597-66vd2mdu	222	20	as	as	IN
cord-014597-66vd2mdu	222	21	a	a	DT
cord-014597-66vd2mdu	222	22	standard	standard	NN
cord-014597-66vd2mdu	222	23	.	.	.
cord-014597-66vd2mdu	223	1	The	the	DT
cord-014597-66vd2mdu	223	2	analysis	analysis	NN
cord-014597-66vd2mdu	223	3	of	of	IN
cord-014597-66vd2mdu	223	4	product	product	NN
cord-014597-66vd2mdu	223	5	quality	quality	NN
cord-014597-66vd2mdu	223	6	was	be	VBD
cord-014597-66vd2mdu	223	7	performed	perform	VBN
cord-014597-66vd2mdu	223	8	by	by	IN
cord-014597-66vd2mdu	223	9	intact	intact	JJ
cord-014597-66vd2mdu	223	10	mass	mass	NN
cord-014597-66vd2mdu	223	11	analysis	analysis	NN
cord-014597-66vd2mdu	223	12	using	use	VBG
cord-014597-66vd2mdu	223	13	reversed	reversed	JJ
cord-014597-66vd2mdu	223	14	phase	phase	NN
cord-014597-66vd2mdu	223	15	chromatography	chromatography	NN
cord-014597-66vd2mdu	223	16	coupled	couple	VBN
cord-014597-66vd2mdu	223	17	to	to	IN
cord-014597-66vd2mdu	223	18	a	a	DT
cord-014597-66vd2mdu	223	19	microOTOF	microotof	NN
cord-014597-66vd2mdu	223	20	-	-	HYPH
cord-014597-66vd2mdu	223	21	Q	Q	NNP
cord-014597-66vd2mdu	223	22	II	II	NNP
cord-014597-66vd2mdu	223	23	mass	mass	NN
cord-014597-66vd2mdu	223	24	spectrometer	spectrometer	NN
cord-014597-66vd2mdu	223	25	(	(	-LRB-
cord-014597-66vd2mdu	223	26	Bruker	Bruker	NNP
cord-014597-66vd2mdu	223	27	Daltonik	Daltonik	NNP
cord-014597-66vd2mdu	223	28	)	)	-RRB-
cord-014597-66vd2mdu	223	29	.	.	.
cord-014597-66vd2mdu	224	1	The	the	DT
cord-014597-66vd2mdu	224	2	CHO	CHO	NNP
cord-014597-66vd2mdu	224	3	cell	cell	NN
cord-014597-66vd2mdu	224	4	culture	culture	NN
cord-014597-66vd2mdu	224	5	cultivated	cultivate	VBN
cord-014597-66vd2mdu	224	6	under	under	IN
cord-014597-66vd2mdu	224	7	low	low	JJ
cord-014597-66vd2mdu	224	8	nutrient	nutrient	NN
cord-014597-66vd2mdu	224	9	conditions	condition	NNS
cord-014597-66vd2mdu	224	10	reached	reach	VBD
cord-014597-66vd2mdu	224	11	a	a	DT
cord-014597-66vd2mdu	224	12	54	54	CD
cord-014597-66vd2mdu	224	13	%	%	NN
cord-014597-66vd2mdu	224	14	higher	high	JJR
cord-014597-66vd2mdu	224	15	viable	viable	JJ
cord-014597-66vd2mdu	224	16	cell	cell	NN
cord-014597-66vd2mdu	224	17	density	density	NN
cord-014597-66vd2mdu	224	18	than	than	IN
cord-014597-66vd2mdu	224	19	the	the	DT
cord-014597-66vd2mdu	224	20	reference	reference	NN
cord-014597-66vd2mdu	224	21	culture	culture	NN
cord-014597-66vd2mdu	224	22	(	(	-LRB-
cord-014597-66vd2mdu	224	23	Fig	Fig	NNP
cord-014597-66vd2mdu	224	24	.	.	.
cord-014597-66vd2mdu	224	25	1a	1a	CD
cord-014597-66vd2mdu	224	26	)	)	-RRB-
cord-014597-66vd2mdu	224	27	.	.	.
cord-014597-66vd2mdu	225	1	The	the	DT
cord-014597-66vd2mdu	225	2	product	product	NN
cord-014597-66vd2mdu	225	3	titer	titer	NN
cord-014597-66vd2mdu	225	4	was	be	VBD
cord-014597-66vd2mdu	225	5	even	even	RB
cord-014597-66vd2mdu	225	6	increased	increase	VBN
cord-014597-66vd2mdu	225	7	by	by	IN
cord-014597-66vd2mdu	225	8	109	109	CD
cord-014597-66vd2mdu	225	9	%	%	NN
cord-014597-66vd2mdu	225	10	(	(	-LRB-
cord-014597-66vd2mdu	225	11	Fig	Fig	NNP
cord-014597-66vd2mdu	225	12	.	.	.
cord-014597-66vd2mdu	225	13	1b	1b	CD
cord-014597-66vd2mdu	225	14	)	)	-RRB-
cord-014597-66vd2mdu	225	15	.	.	.
cord-014597-66vd2mdu	226	1	The	the	DT
cord-014597-66vd2mdu	226	2	spent	spend	VBN
cord-014597-66vd2mdu	226	3	media	medium	NNS
cord-014597-66vd2mdu	226	4	analysis	analysis	NN
cord-014597-66vd2mdu	226	5	shows	show	VBZ
cord-014597-66vd2mdu	226	6	that	that	IN
cord-014597-66vd2mdu	226	7	some	some	DT
cord-014597-66vd2mdu	226	8	amino	amino	JJ
cord-014597-66vd2mdu	226	9	acids	acid	NNS
cord-014597-66vd2mdu	226	10	and	and	CC
cord-014597-66vd2mdu	226	11	vitamins	vitamin	NNS
cord-014597-66vd2mdu	226	12	were	be	VBD
cord-014597-66vd2mdu	226	13	present	present	JJ
cord-014597-66vd2mdu	226	14	at	at	IN
cord-014597-66vd2mdu	226	15	presumably	presumably	RB
cord-014597-66vd2mdu	226	16	limiting	limit	VBG
cord-014597-66vd2mdu	226	17	concentrations	concentration	NNS
cord-014597-66vd2mdu	226	18	after	after	IN
cord-014597-66vd2mdu	226	19	day	day	NN
cord-014597-66vd2mdu	226	20	5/6	5/6	CD
cord-014597-66vd2mdu	226	21	,	,	,
cord-014597-66vd2mdu	226	22	mostly	mostly	RB
cord-014597-66vd2mdu	226	23	in	in	IN
cord-014597-66vd2mdu	226	24	the	the	DT
cord-014597-66vd2mdu	226	25	low	low	JJ
cord-014597-66vd2mdu	226	26	nutrient	nutrient	NN
cord-014597-66vd2mdu	226	27	level	level	NN
cord-014597-66vd2mdu	226	28	culture	culture	NN
cord-014597-66vd2mdu	226	29	(	(	-LRB-
cord-014597-66vd2mdu	226	30	down	down	IN
cord-014597-66vd2mdu	226	31	to	to	IN
cord-014597-66vd2mdu	226	32	40	40	CD
cord-014597-66vd2mdu	226	33	to	to	TO
cord-014597-66vd2mdu	226	34	190	190	CD
cord-014597-66vd2mdu	226	35	μM	μm	NN
cord-014597-66vd2mdu	226	36	for	for	IN
cord-014597-66vd2mdu	226	37	TYR	TYR	NNP
cord-014597-66vd2mdu	226	38	,	,	,
cord-014597-66vd2mdu	226	39	GLN	GLN	NNP
cord-014597-66vd2mdu	226	40	,	,	,
cord-014597-66vd2mdu	226	41	ARG	ARG	NNP
cord-014597-66vd2mdu	226	42	,	,	,
cord-014597-66vd2mdu	226	43	and	and	CC
cord-014597-66vd2mdu	226	44	ASN	ASN	NNP
cord-014597-66vd2mdu	226	45	,	,	,
cord-014597-66vd2mdu	226	46	below	below	IN
cord-014597-66vd2mdu	226	47	1	1	CD
cord-014597-66vd2mdu	226	48	μM	μm	NN
cord-014597-66vd2mdu	226	49	for	for	IN
cord-014597-66vd2mdu	226	50	pyridoxine	pyridoxine	NN
cord-014597-66vd2mdu	226	51	,	,	,
cord-014597-66vd2mdu	226	52	data	datum	NNS
cord-014597-66vd2mdu	226	53	not	not	RB
cord-014597-66vd2mdu	226	54	shown	show	VBN
cord-014597-66vd2mdu	226	55	)	)	-RRB-
cord-014597-66vd2mdu	226	56	.	.	.
cord-014597-66vd2mdu	227	1	The	the	DT
cord-014597-66vd2mdu	227	2	product	product	NN
cord-014597-66vd2mdu	227	3	quality	quality	NN
cord-014597-66vd2mdu	227	4	showed	show	VBD
cord-014597-66vd2mdu	227	5	significant	significant	JJ
cord-014597-66vd2mdu	227	6	changes	change	NNS
cord-014597-66vd2mdu	227	7	for	for	IN
cord-014597-66vd2mdu	227	8	the	the	DT
cord-014597-66vd2mdu	227	9	changed	change	VBN
cord-014597-66vd2mdu	227	10	feeding	feeding	NN
cord-014597-66vd2mdu	227	11	strategy	strategy	NN
cord-014597-66vd2mdu	227	12	(	(	-LRB-
cord-014597-66vd2mdu	227	13	Fig	Fig	NNP
cord-014597-66vd2mdu	227	14	.	.	.
cord-014597-66vd2mdu	227	15	1c	1c	NNP
cord-014597-66vd2mdu	227	16	and	and	CC
cord-014597-66vd2mdu	227	17	d	d	NNP
cord-014597-66vd2mdu	227	18	)	)	-RRB-
cord-014597-66vd2mdu	227	19	.	.	.
cord-014597-66vd2mdu	228	1	As	as	IN
cord-014597-66vd2mdu	228	2	expected	expect	VBN
cord-014597-66vd2mdu	228	3	,	,	,
cord-014597-66vd2mdu	228	4	the	the	DT
cord-014597-66vd2mdu	228	5	glycation	glycation	NN
cord-014597-66vd2mdu	228	6	level	level	NN
cord-014597-66vd2mdu	228	7	decreased	decrease	VBD
cord-014597-66vd2mdu	228	8	from	from	IN
cord-014597-66vd2mdu	228	9	3	3	CD
cord-014597-66vd2mdu	228	10	%	%	NN
cord-014597-66vd2mdu	228	11	to	to	TO
cord-014597-66vd2mdu	228	12	1	1	CD
cord-014597-66vd2mdu	228	13	%	%	NN
cord-014597-66vd2mdu	228	14	compared	compare	VBN
cord-014597-66vd2mdu	228	15	to	to	IN
cord-014597-66vd2mdu	228	16	the	the	DT
cord-014597-66vd2mdu	228	17	reference	reference	NN
cord-014597-66vd2mdu	228	18	culture	culture	NN
cord-014597-66vd2mdu	228	19	.	.	.
cord-014597-66vd2mdu	229	1	The	the	DT
cord-014597-66vd2mdu	229	2	truncation	truncation	NN
cord-014597-66vd2mdu	229	3	level	level	NN
cord-014597-66vd2mdu	229	4	of	of	IN
cord-014597-66vd2mdu	229	5	C	c	NN
cord-014597-66vd2mdu	229	6	-	-	HYPH
cord-014597-66vd2mdu	229	7	terminal	terminal	NN
cord-014597-66vd2mdu	229	8	lysine	lysine	NN
cord-014597-66vd2mdu	229	9	at	at	IN
cord-014597-66vd2mdu	229	10	the	the	DT
cord-014597-66vd2mdu	229	11	heavy	heavy	JJ
cord-014597-66vd2mdu	229	12	chain	chain	NN
cord-014597-66vd2mdu	229	13	of	of	IN
cord-014597-66vd2mdu	229	14	the	the	DT
cord-014597-66vd2mdu	229	15	mAB	mAB	NNPS
cord-014597-66vd2mdu	229	16	increased	increase	VBD
cord-014597-66vd2mdu	229	17	from	from	IN
cord-014597-66vd2mdu	229	18	79	79	CD
cord-014597-66vd2mdu	229	19	%	%	NN
cord-014597-66vd2mdu	229	20	to	to	IN
cord-014597-66vd2mdu	229	21	88	88	CD
cord-014597-66vd2mdu	229	22	%	%	NN
cord-014597-66vd2mdu	229	23	.	.	.
cord-014597-66vd2mdu	230	1	The	the	DT
cord-014597-66vd2mdu	230	2	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	230	3	was	be	VBD
cord-014597-66vd2mdu	230	4	also	also	RB
cord-014597-66vd2mdu	230	5	significantly	significantly	RB
cord-014597-66vd2mdu	230	6	influenced	influence	VBN
cord-014597-66vd2mdu	230	7	by	by	IN
cord-014597-66vd2mdu	230	8	the	the	DT
cord-014597-66vd2mdu	230	9	low	low	JJ
cord-014597-66vd2mdu	230	10	nutrient	nutrient	NN
cord-014597-66vd2mdu	230	11	level	level	NN
cord-014597-66vd2mdu	230	12	(	(	-LRB-
cord-014597-66vd2mdu	230	13	Fig	Fig	NNP
cord-014597-66vd2mdu	230	14	.	.	.
cord-014597-66vd2mdu	230	15	1e	1e	NNP
cord-014597-66vd2mdu	230	16	)	)	-RRB-
cord-014597-66vd2mdu	230	17	:	:	:
cord-014597-66vd2mdu	231	1	The	the	DT
cord-014597-66vd2mdu	231	2	nonfucosylated	nonfucosylated	JJ
cord-014597-66vd2mdu	231	3	variants	variant	NNS
cord-014597-66vd2mdu	231	4	increased	increase	VBD
cord-014597-66vd2mdu	231	5	from	from	IN
cord-014597-66vd2mdu	231	6	3	3	CD
cord-014597-66vd2mdu	231	7	%	%	NN
cord-014597-66vd2mdu	231	8	to	to	TO
cord-014597-66vd2mdu	231	9	6	6	CD
cord-014597-66vd2mdu	231	10	%	%	NN
cord-014597-66vd2mdu	231	11	(	(	-LRB-
cord-014597-66vd2mdu	231	12	Fig	Fig	NNP
cord-014597-66vd2mdu	231	13	.	.	.
cord-014597-66vd2mdu	231	14	1f	1f	CD
cord-014597-66vd2mdu	231	15	)	)	-RRB-
cord-014597-66vd2mdu	231	16	,	,	,
cord-014597-66vd2mdu	231	17	the	the	DT
cord-014597-66vd2mdu	231	18	degree	degree	NN
cord-014597-66vd2mdu	231	19	of	of	IN
cord-014597-66vd2mdu	231	20	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	231	21	increased	increase	VBD
cord-014597-66vd2mdu	231	22	from	from	IN
cord-014597-66vd2mdu	231	23	31	31	CD
cord-014597-66vd2mdu	231	24	%	%	NN
cord-014597-66vd2mdu	231	25	to	to	IN
cord-014597-66vd2mdu	231	26	39	39	CD
cord-014597-66vd2mdu	231	27	%	%	NN
cord-014597-66vd2mdu	231	28	(	(	-LRB-
cord-014597-66vd2mdu	231	29	Fig	Fig	NNP
cord-014597-66vd2mdu	231	30	.	.	.
cord-014597-66vd2mdu	231	31	1	1	CD
cord-014597-66vd2mdu	231	32	g	g	NN
cord-014597-66vd2mdu	231	33	)	)	-RRB-
cord-014597-66vd2mdu	231	34	.	.	.
cord-014597-66vd2mdu	232	1	Cultivation	cultivation	NN
cord-014597-66vd2mdu	232	2	under	under	IN
cord-014597-66vd2mdu	232	3	low	low	JJ
cord-014597-66vd2mdu	232	4	nutrient	nutrient	NN
cord-014597-66vd2mdu	232	5	level	level	NN
cord-014597-66vd2mdu	232	6	led	lead	VBD
cord-014597-66vd2mdu	232	7	to	to	IN
cord-014597-66vd2mdu	232	8	54	54	CD
cord-014597-66vd2mdu	232	9	%	%	NN
cord-014597-66vd2mdu	232	10	higher	high	JJR
cord-014597-66vd2mdu	232	11	viable	viable	JJ
cord-014597-66vd2mdu	232	12	cell	cell	NN
cord-014597-66vd2mdu	232	13	density	density	NN
cord-014597-66vd2mdu	232	14	and	and	CC
cord-014597-66vd2mdu	233	1	a	a	DT
cord-014597-66vd2mdu	233	2	product	product	NN
cord-014597-66vd2mdu	233	3	titer	titer	NN
cord-014597-66vd2mdu	233	4	increased	increase	VBD
cord-014597-66vd2mdu	233	5	by	by	IN
cord-014597-66vd2mdu	233	6	109	109	CD
cord-014597-66vd2mdu	233	7	%	%	NN
cord-014597-66vd2mdu	233	8	when	when	WRB
cord-014597-66vd2mdu	233	9	compared	compare	VBN
cord-014597-66vd2mdu	233	10	to	to	IN
cord-014597-66vd2mdu	233	11	reference	reference	NN
cord-014597-66vd2mdu	233	12	culture	culture	NN
cord-014597-66vd2mdu	233	13	grown	grow	VBN
cord-014597-66vd2mdu	233	14	under	under	IN
cord-014597-66vd2mdu	233	15	non	non	JJ
cord-014597-66vd2mdu	233	16	-	-	JJ
cord-014597-66vd2mdu	233	17	limiting	limit	VBG
cord-014597-66vd2mdu	233	18	nutrient	nutrient	NN
cord-014597-66vd2mdu	233	19	conditions	condition	NNS
cord-014597-66vd2mdu	233	20	.	.	.
cord-014597-66vd2mdu	234	1	The	the	DT
cord-014597-66vd2mdu	234	2	analysis	analysis	NN
cord-014597-66vd2mdu	234	3	of	of	IN
cord-014597-66vd2mdu	234	4	product	product	NN
cord-014597-66vd2mdu	234	5	quality	quality	NN
cord-014597-66vd2mdu	234	6	reveals	reveal	VBZ
cord-014597-66vd2mdu	234	7	75	75	CD
cord-014597-66vd2mdu	234	8	%	%	NN
cord-014597-66vd2mdu	234	9	less	less	JJR
cord-014597-66vd2mdu	234	10	glycation	glycation	NN
cord-014597-66vd2mdu	234	11	of	of	IN
cord-014597-66vd2mdu	234	12	light	light	JJ
cord-014597-66vd2mdu	234	13	chain	chain	NN
cord-014597-66vd2mdu	234	14	for	for	IN
cord-014597-66vd2mdu	234	15	CHO	CHO	NNP
cord-014597-66vd2mdu	234	16	cells	cell	NNS
cord-014597-66vd2mdu	234	17	grown	grow	VBN
cord-014597-66vd2mdu	234	18	under	under	IN
cord-014597-66vd2mdu	234	19	low	low	JJ
cord-014597-66vd2mdu	234	20	nutrient	nutrient	NN
cord-014597-66vd2mdu	234	21	conditions	condition	NNS
cord-014597-66vd2mdu	234	22	(	(	-LRB-
cord-014597-66vd2mdu	234	23	0.7	0.7	CD
cord-014597-66vd2mdu	234	24	%	%	NN
cord-014597-66vd2mdu	234	25	vs	vs	IN
cord-014597-66vd2mdu	234	26	2.7	2.7	CD
cord-014597-66vd2mdu	234	27	%	%	NN
cord-014597-66vd2mdu	234	28	in	in	IN
cord-014597-66vd2mdu	234	29	reference	reference	NN
cord-014597-66vd2mdu	234	30	culture	culture	NN
cord-014597-66vd2mdu	234	31	)	)	-RRB-
cord-014597-66vd2mdu	234	32	.	.	.
cord-014597-66vd2mdu	235	1	The	the	DT
cord-014597-66vd2mdu	235	2	truncation	truncation	NN
cord-014597-66vd2mdu	235	3	of	of	IN
cord-014597-66vd2mdu	235	4	C	c	NN
cord-014597-66vd2mdu	235	5	-	-	HYPH
cord-014597-66vd2mdu	235	6	terminal	terminal	NN
cord-014597-66vd2mdu	235	7	lysine	lysine	NN
cord-014597-66vd2mdu	235	8	decreased	decrease	VBD
cord-014597-66vd2mdu	235	9	by	by	IN
cord-014597-66vd2mdu	235	10	10	10	CD
cord-014597-66vd2mdu	235	11	%	%	NN
cord-014597-66vd2mdu	235	12	(	(	-LRB-
cord-014597-66vd2mdu	235	13	from	from	IN
cord-014597-66vd2mdu	235	14	88	88	CD
cord-014597-66vd2mdu	235	15	%	%	NN
cord-014597-66vd2mdu	235	16	to	to	IN
cord-014597-66vd2mdu	235	17	79	79	CD
cord-014597-66vd2mdu	235	18	%	%	NN
cord-014597-66vd2mdu	235	19	)	)	-RRB-
cord-014597-66vd2mdu	235	20	,	,	,
cord-014597-66vd2mdu	235	21	the	the	DT
cord-014597-66vd2mdu	235	22	degree	degree	NN
cord-014597-66vd2mdu	235	23	of	of	IN
cord-014597-66vd2mdu	235	24	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	235	25	increased	increase	VBN
cord-014597-66vd2mdu	235	26	by	by	IN
cord-014597-66vd2mdu	235	27	23	23	CD
cord-014597-66vd2mdu	235	28	%	%	NN
cord-014597-66vd2mdu	235	29	(	(	-LRB-
cord-014597-66vd2mdu	235	30	from	from	IN
cord-014597-66vd2mdu	235	31	31	31	CD
cord-014597-66vd2mdu	235	32	%	%	NN
cord-014597-66vd2mdu	235	33	to	to	IN
cord-014597-66vd2mdu	235	34	39	39	CD
cord-014597-66vd2mdu	235	35	%	%	NN
cord-014597-66vd2mdu	235	36	,	,	,
cord-014597-66vd2mdu	235	37	also	also	RB
cord-014597-66vd2mdu	235	38	observed	observe	VBN
cord-014597-66vd2mdu	235	39	by	by	IN
cord-014597-66vd2mdu	235	40	Takuma	Takuma	NNP
cord-014597-66vd2mdu	235	41	et	et	NNP
cord-014597-66vd2mdu	235	42	al	al	NNP
cord-014597-66vd2mdu	235	43	.	.	.
cord-014597-66vd2mdu	236	1	[	[	-LRB-
cord-014597-66vd2mdu	236	2	2	2	CD
cord-014597-66vd2mdu	236	3	]	]	-RRB-
cord-014597-66vd2mdu	236	4	)	)	-RRB-
cord-014597-66vd2mdu	237	1	and	and	CC
cord-014597-66vd2mdu	237	2	non	non	JJ
cord-014597-66vd2mdu	237	3	-	-	JJ
cord-014597-66vd2mdu	237	4	fucosylated	fucosylated	JJ
cord-014597-66vd2mdu	237	5	glycans	glycan	NNS
cord-014597-66vd2mdu	237	6	increased	increase	VBN
cord-014597-66vd2mdu	237	7	by	by	IN
cord-014597-66vd2mdu	237	8	105	105	CD
cord-014597-66vd2mdu	237	9	%	%	NN
cord-014597-66vd2mdu	237	10	(	(	-LRB-
cord-014597-66vd2mdu	237	11	from	from	IN
cord-014597-66vd2mdu	237	12	2.8	2.8	CD
cord-014597-66vd2mdu	237	13	%	%	NN
cord-014597-66vd2mdu	237	14	to	to	IN
cord-014597-66vd2mdu	237	15	5.8	5.8	CD
cord-014597-66vd2mdu	237	16	%	%	NN
cord-014597-66vd2mdu	237	17	)	)	-RRB-
cord-014597-66vd2mdu	237	18	under	under	IN
cord-014597-66vd2mdu	237	19	low	low	JJ
cord-014597-66vd2mdu	237	20	nutrient	nutrient	NN
cord-014597-66vd2mdu	237	21	conditions	condition	NNS
cord-014597-66vd2mdu	237	22	.	.	.
cord-014597-66vd2mdu	238	1	The	the	DT
cord-014597-66vd2mdu	238	2	product	product	NN
cord-014597-66vd2mdu	238	3	quality	quality	NN
cord-014597-66vd2mdu	238	4	analysis	analysis	NN
cord-014597-66vd2mdu	238	5	by	by	IN
cord-014597-66vd2mdu	238	6	intact	intact	JJ
cord-014597-66vd2mdu	238	7	mass	mass	NN
cord-014597-66vd2mdu	238	8	proved	prove	VBD
cord-014597-66vd2mdu	238	9	to	to	TO
cord-014597-66vd2mdu	238	10	be	be	VB
cord-014597-66vd2mdu	238	11	highly	highly	RB
cord-014597-66vd2mdu	238	12	robust	robust	JJ
cord-014597-66vd2mdu	238	13	(	(	-LRB-
cord-014597-66vd2mdu	238	14	average	average	JJ
cord-014597-66vd2mdu	238	15	CV	cv	NN
cord-014597-66vd2mdu	238	16	for	for	IN
cord-014597-66vd2mdu	238	17	four	four	CD
cord-014597-66vd2mdu	238	18	replicates	replicate	NNS
cord-014597-66vd2mdu	238	19	=	=	SYM
cord-014597-66vd2mdu	238	20	2	2	CD
cord-014597-66vd2mdu	238	21	%	%	NN
cord-014597-66vd2mdu	238	22	)	)	-RRB-
cord-014597-66vd2mdu	238	23	.	.	.
cord-014597-66vd2mdu	239	1	In	in	IN
cord-014597-66vd2mdu	239	2	summary	summary	NN
cord-014597-66vd2mdu	239	3	,	,	,
cord-014597-66vd2mdu	239	4	cultivation	cultivation	NN
cord-014597-66vd2mdu	239	5	with	with	IN
cord-014597-66vd2mdu	239	6	alternative	alternative	JJ
cord-014597-66vd2mdu	239	7	feed	feed	NN
cord-014597-66vd2mdu	239	8	led	lead	VBD
cord-014597-66vd2mdu	239	9	to	to	IN
cord-014597-66vd2mdu	239	10	higher	high	JJR
cord-014597-66vd2mdu	239	11	IgG	igg	NN
cord-014597-66vd2mdu	239	12	product	product	NN
cord-014597-66vd2mdu	239	13	titer	titer	NN
cord-014597-66vd2mdu	239	14	and	and	CC
cord-014597-66vd2mdu	239	15	better	well	JJR
cord-014597-66vd2mdu	239	16	product	product	NN
cord-014597-66vd2mdu	239	17	quality	quality	NN
cord-014597-66vd2mdu	239	18	(	(	-LRB-
cord-014597-66vd2mdu	239	19	glycation	glycation	NN
cord-014597-66vd2mdu	239	20	unwanted	unwanted	JJ
cord-014597-66vd2mdu	239	21	,	,	,
cord-014597-66vd2mdu	239	22	higher	high	JJR
cord-014597-66vd2mdu	239	23	amount	amount	NN
cord-014597-66vd2mdu	239	24	of	of	IN
cord-014597-66vd2mdu	239	25	non	non	JJ
cord-014597-66vd2mdu	239	26	-	-	JJ
cord-014597-66vd2mdu	239	27	fucosylated	fucosylated	JJ
cord-014597-66vd2mdu	239	28	glycans	glycan	NNS
cord-014597-66vd2mdu	239	29	leads	lead	VBZ
cord-014597-66vd2mdu	239	30	to	to	IN
cord-014597-66vd2mdu	239	31	higher	high	JJR
cord-014597-66vd2mdu	239	32	antibody	antibody	NN
cord-014597-66vd2mdu	239	33	-	-	HYPH
cord-014597-66vd2mdu	239	34	dependent	dependent	JJ
cord-014597-66vd2mdu	239	35	cellmediated	cellmediate	VBN
cord-014597-66vd2mdu	239	36	cytotoxicity	cytotoxicity	NN
cord-014597-66vd2mdu	239	37	(	(	-LRB-
cord-014597-66vd2mdu	239	38	ADCC	ADCC	NNP
cord-014597-66vd2mdu	239	39	)	)	-RRB-
cord-014597-66vd2mdu	239	40	,	,	,
cord-014597-66vd2mdu	239	41	higher	high	JJR
cord-014597-66vd2mdu	239	42	amount	amount	NN
cord-014597-66vd2mdu	239	43	of	of	IN
cord-014597-66vd2mdu	239	44	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	239	45	to	to	IN
cord-014597-66vd2mdu	239	46	higher	high	JJR
cord-014597-66vd2mdu	239	47	complement	complement	NN
cord-014597-66vd2mdu	239	48	-	-	HYPH
cord-014597-66vd2mdu	239	49	dependent	dependent	JJ
cord-014597-66vd2mdu	239	50	cytotoxicity	cytotoxicity	NN
cord-014597-66vd2mdu	239	51	(	(	-LRB-
cord-014597-66vd2mdu	239	52	CDC	CDC	NNP
cord-014597-66vd2mdu	239	53	)	)	-RRB-
cord-014597-66vd2mdu	239	54	and	and	CC
cord-014597-66vd2mdu	239	55	ADCC	ADCC	NNP
cord-014597-66vd2mdu	239	56	[	[	-LRB-
cord-014597-66vd2mdu	239	57	3	3	CD
cord-014597-66vd2mdu	239	58	,	,	,
cord-014597-66vd2mdu	239	59	4	4	CD
cord-014597-66vd2mdu	239	60	]	]	-RRB-
cord-014597-66vd2mdu	239	61	)	)	-RRB-
cord-014597-66vd2mdu	239	62	.	.	.
cord-014597-66vd2mdu	240	1	Background	background	NN
cord-014597-66vd2mdu	241	1	We	-PRON-	PRP
cord-014597-66vd2mdu	241	2	developed	develop	VBD
cord-014597-66vd2mdu	241	3	an	an	DT
cord-014597-66vd2mdu	241	4	automated	automate	VBN
cord-014597-66vd2mdu	241	5	,	,	,
cord-014597-66vd2mdu	241	6	multiwell	multiwell	NNP
cord-014597-66vd2mdu	241	7	plate	plate	NN
cord-014597-66vd2mdu	241	8	(	(	-LRB-
cord-014597-66vd2mdu	241	9	MWP	MWP	NNP
cord-014597-66vd2mdu	241	10	)	)	-RRB-
cord-014597-66vd2mdu	241	11	based	base	VBN
cord-014597-66vd2mdu	241	12	screening	screening	NN
cord-014597-66vd2mdu	241	13	system	system	NN
cord-014597-66vd2mdu	241	14	for	for	IN
cord-014597-66vd2mdu	241	15	suspension	suspension	NN
cord-014597-66vd2mdu	241	16	cell	cell	NN
cord-014597-66vd2mdu	241	17	cultures	culture	NNS
cord-014597-66vd2mdu	241	18	(	(	-LRB-
cord-014597-66vd2mdu	241	19	Fig	Fig	NNP
cord-014597-66vd2mdu	241	20	.	.	.
cord-014597-66vd2mdu	241	21	1a	1a	LS
cord-014597-66vd2mdu	241	22	)	)	-RRB-
cord-014597-66vd2mdu	241	23	which	which	WDT
cord-014597-66vd2mdu	241	24	is	be	VBZ
cord-014597-66vd2mdu	241	25	now	now	RB
cord-014597-66vd2mdu	241	26	routinely	routinely	RB
cord-014597-66vd2mdu	241	27	used	use	VBN
cord-014597-66vd2mdu	241	28	in	in	IN
cord-014597-66vd2mdu	241	29	cell	cell	NN
cord-014597-66vd2mdu	241	30	culture	culture	NN
cord-014597-66vd2mdu	241	31	process	process	NN
cord-014597-66vd2mdu	241	32	development	development	NN
cord-014597-66vd2mdu	241	33	.	.	.
cord-014597-66vd2mdu	242	1	It	-PRON-	PRP
cord-014597-66vd2mdu	242	2	is	be	VBZ
cord-014597-66vd2mdu	242	3	characterized	characterize	VBN
cord-014597-66vd2mdu	242	4	by	by	IN
cord-014597-66vd2mdu	242	5	a	a	DT
cord-014597-66vd2mdu	242	6	fully	fully	RB
cord-014597-66vd2mdu	242	7	automated	automate	VBN
cord-014597-66vd2mdu	242	8	workflow	workflow	NN
cord-014597-66vd2mdu	242	9	with	with	IN
cord-014597-66vd2mdu	242	10	integrated	integrate	VBN
cord-014597-66vd2mdu	242	11	analytical	analytical	JJ
cord-014597-66vd2mdu	242	12	instrumentation	instrumentation	NN
cord-014597-66vd2mdu	242	13	.	.	.
cord-014597-66vd2mdu	243	1	It	-PRON-	PRP
cord-014597-66vd2mdu	243	2	uses	use	VBZ
cord-014597-66vd2mdu	243	3	shaken	shake	VBN
cord-014597-66vd2mdu	243	4	6	6	CD
cord-014597-66vd2mdu	243	5	-	-	SYM
cord-014597-66vd2mdu	243	6	24	24	CD
cord-014597-66vd2mdu	243	7	well	well	NN
cord-014597-66vd2mdu	243	8	plates	plate	NNS
cord-014597-66vd2mdu	243	9	as	as	IN
cord-014597-66vd2mdu	243	10	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	243	11	which	which	WDT
cord-014597-66vd2mdu	243	12	can	can	MD
cord-014597-66vd2mdu	243	13	be	be	VB
cord-014597-66vd2mdu	243	14	run	run	VBN
cord-014597-66vd2mdu	243	15	in	in	IN
cord-014597-66vd2mdu	243	16	batch	batch	NN
cord-014597-66vd2mdu	243	17	and	and	CC
cord-014597-66vd2mdu	243	18	fed	fed	JJ
cord-014597-66vd2mdu	243	19	-	-	HYPH
cord-014597-66vd2mdu	243	20	batch	batch	NN
cord-014597-66vd2mdu	243	21	mode	mode	NN
cord-014597-66vd2mdu	243	22	with	with	IN
cord-014597-66vd2mdu	243	23	a	a	DT
cord-014597-66vd2mdu	243	24	capacity	capacity	NN
cord-014597-66vd2mdu	243	25	of	of	IN
cord-014597-66vd2mdu	243	26	up	up	IN
cord-014597-66vd2mdu	243	27	to	to	TO
cord-014597-66vd2mdu	243	28	768	768	CD
cord-014597-66vd2mdu	243	29	reactors	reactor	NNS
cord-014597-66vd2mdu	243	30	in	in	IN
cord-014597-66vd2mdu	243	31	parallel	parallel	NN
cord-014597-66vd2mdu	243	32	[	[	-LRB-
cord-014597-66vd2mdu	243	33	1	1	CD
cord-014597-66vd2mdu	243	34	]	]	-RRB-
cord-014597-66vd2mdu	243	35	[	[	-LRB-
cord-014597-66vd2mdu	243	36	2	2	CD
cord-014597-66vd2mdu	243	37	]	]	-RRB-
cord-014597-66vd2mdu	243	38	[	[	-LRB-
cord-014597-66vd2mdu	243	39	3	3	CD
cord-014597-66vd2mdu	243	40	]	]	-RRB-
cord-014597-66vd2mdu	243	41	.	.	.
cord-014597-66vd2mdu	244	1	A	a	DT
cord-014597-66vd2mdu	244	2	wide	wide	JJ
cord-014597-66vd2mdu	244	3	ranging	range	VBG
cord-014597-66vd2mdu	244	4	analytical	analytical	JJ
cord-014597-66vd2mdu	244	5	portfolio	portfolio	NN
cord-014597-66vd2mdu	244	6	to	to	TO
cord-014597-66vd2mdu	244	7	monitor	monitor	VB
cord-014597-66vd2mdu	244	8	cell	cell	NN
cord-014597-66vd2mdu	244	9	culture	culture	NN
cord-014597-66vd2mdu	244	10	processes	process	NNS
cord-014597-66vd2mdu	244	11	and	and	CC
cord-014597-66vd2mdu	244	12	also	also	RB
cord-014597-66vd2mdu	244	13	a	a	DT
cord-014597-66vd2mdu	244	14	cooperation	cooperation	NN
cord-014597-66vd2mdu	244	15	with	with	IN
cord-014597-66vd2mdu	244	16	internal	internal	JJ
cord-014597-66vd2mdu	244	17	high	high	JJ
cord-014597-66vd2mdu	244	18	throughput	throughput	NN
cord-014597-66vd2mdu	244	19	(	(	-LRB-
cord-014597-66vd2mdu	244	20	HT	HT	NNP
cord-014597-66vd2mdu	244	21	)	)	-RRB-
cord-014597-66vd2mdu	244	22	analytic	analytic	JJ
cord-014597-66vd2mdu	244	23	groups	group	NNS
cord-014597-66vd2mdu	244	24	to	to	TO
cord-014597-66vd2mdu	244	25	characterize	characterize	VB
cord-014597-66vd2mdu	244	26	product	product	NN
cord-014597-66vd2mdu	244	27	quality	quality	NN
cord-014597-66vd2mdu	244	28	are	be	VBP
cord-014597-66vd2mdu	244	29	available	available	JJ
cord-014597-66vd2mdu	244	30	.	.	.
cord-014597-66vd2mdu	245	1	In	in	IN
cord-014597-66vd2mdu	245	2	addition	addition	NN
cord-014597-66vd2mdu	245	3	the	the	DT
cord-014597-66vd2mdu	245	4	use	use	NN
cord-014597-66vd2mdu	245	5	and	and	CC
cord-014597-66vd2mdu	245	6	the	the	DT
cord-014597-66vd2mdu	245	7	benefits	benefit	NNS
cord-014597-66vd2mdu	245	8	of	of	IN
cord-014597-66vd2mdu	245	9	spectroscopic	spectroscopic	JJ
cord-014597-66vd2mdu	245	10	methods	method	NNS
cord-014597-66vd2mdu	245	11	for	for	IN
cord-014597-66vd2mdu	245	12	cell	cell	NN
cord-014597-66vd2mdu	245	13	culture	culture	NN
cord-014597-66vd2mdu	245	14	automation	automation	NN
cord-014597-66vd2mdu	245	15	were	be	VBD
cord-014597-66vd2mdu	245	16	shown	show	VBN
cord-014597-66vd2mdu	245	17	in	in	IN
cord-014597-66vd2mdu	245	18	the	the	DT
cord-014597-66vd2mdu	245	19	past	past	NN
cord-014597-66vd2mdu	245	20	[	[	-LRB-
cord-014597-66vd2mdu	245	21	4	4	CD
cord-014597-66vd2mdu	245	22	,	,	,
cord-014597-66vd2mdu	245	23	5	5	CD
cord-014597-66vd2mdu	245	24	]	]	-RRB-
cord-014597-66vd2mdu	245	25	.	.	.
cord-014597-66vd2mdu	246	1	Automated	automated	JJ
cord-014597-66vd2mdu	246	2	cell	cell	NN
cord-014597-66vd2mdu	246	3	culture	culture	NN
cord-014597-66vd2mdu	246	4	systems	system	NNS
cord-014597-66vd2mdu	246	5	enable	enable	VBP
cord-014597-66vd2mdu	246	6	broader	broad	JJR
cord-014597-66vd2mdu	246	7	screening	screening	NN
cord-014597-66vd2mdu	246	8	within	within	IN
cord-014597-66vd2mdu	246	9	a	a	DT
cord-014597-66vd2mdu	246	10	shorter	short	JJR
cord-014597-66vd2mdu	246	11	time	time	NN
cord-014597-66vd2mdu	246	12	frame	frame	NN
cord-014597-66vd2mdu	246	13	for	for	IN
cord-014597-66vd2mdu	246	14	many	many	JJ
cord-014597-66vd2mdu	246	15	applications	application	NNS
cord-014597-66vd2mdu	246	16	in	in	IN
cord-014597-66vd2mdu	246	17	upstream	upstream	JJ
cord-014597-66vd2mdu	246	18	process	process	NN
cord-014597-66vd2mdu	246	19	development	development	NN
cord-014597-66vd2mdu	246	20	.	.	.
cord-014597-66vd2mdu	247	1	The	the	DT
cord-014597-66vd2mdu	247	2	higher	high	JJR
cord-014597-66vd2mdu	247	3	degree	degree	NN
cord-014597-66vd2mdu	247	4	of	of	IN
cord-014597-66vd2mdu	247	5	parallelization	parallelization	NN
cord-014597-66vd2mdu	247	6	and	and	CC
cord-014597-66vd2mdu	247	7	automation	automation	NN
cord-014597-66vd2mdu	247	8	helps	help	VBZ
cord-014597-66vd2mdu	247	9	to	to	TO
cord-014597-66vd2mdu	247	10	screen	screen	VB
cord-014597-66vd2mdu	247	11	for	for	IN
cord-014597-66vd2mdu	247	12	most	most	RBS
cord-014597-66vd2mdu	247	13	promising	promising	JJ
cord-014597-66vd2mdu	247	14	parameters	parameter	NNS
cord-014597-66vd2mdu	247	15	in	in	IN
cord-014597-66vd2mdu	247	16	a	a	DT
cord-014597-66vd2mdu	247	17	shorter	short	JJR
cord-014597-66vd2mdu	247	18	time	time	NN
cord-014597-66vd2mdu	247	19	.	.	.
cord-014597-66vd2mdu	248	1	The	the	DT
cord-014597-66vd2mdu	248	2	use	use	NN
cord-014597-66vd2mdu	248	3	of	of	IN
cord-014597-66vd2mdu	248	4	broad	broad	JJ
cord-014597-66vd2mdu	248	5	DoE	DoE	NNP
cord-014597-66vd2mdu	248	6	screening	screening	NN
cord-014597-66vd2mdu	248	7	design	design	NN
cord-014597-66vd2mdu	248	8	allows	allow	VBZ
cord-014597-66vd2mdu	248	9	in	in	IN
cord-014597-66vd2mdu	248	10	addition	addition	NN
cord-014597-66vd2mdu	248	11	the	the	DT
cord-014597-66vd2mdu	248	12	identification	identification	NN
cord-014597-66vd2mdu	248	13	of	of	IN
cord-014597-66vd2mdu	248	14	parameters	parameter	NNS
cord-014597-66vd2mdu	248	15	that	that	WDT
cord-014597-66vd2mdu	248	16	support	support	VBP
cord-014597-66vd2mdu	248	17	high	high	JJ
cord-014597-66vd2mdu	248	18	titers	titer	NNS
cord-014597-66vd2mdu	248	19	while	while	IN
cord-014597-66vd2mdu	248	20	keeping	keep	VBG
cord-014597-66vd2mdu	248	21	high	high	JJ
cord-014597-66vd2mdu	248	22	product	product	NN
cord-014597-66vd2mdu	248	23	quality	quality	NN
cord-014597-66vd2mdu	248	24	(	(	-LRB-
cord-014597-66vd2mdu	248	25	multiple	multiple	JJ
cord-014597-66vd2mdu	248	26	factors	factor	NNS
cord-014597-66vd2mdu	248	27	at	at	IN
cord-014597-66vd2mdu	248	28	the	the	DT
cord-014597-66vd2mdu	248	29	same	same	JJ
cord-014597-66vd2mdu	248	30	time	time	NN
cord-014597-66vd2mdu	248	31	)	)	-RRB-
cord-014597-66vd2mdu	248	32	.	.	.
cord-014597-66vd2mdu	249	1	The	the	DT
cord-014597-66vd2mdu	249	2	illustration	illustration	NN
cord-014597-66vd2mdu	249	3	(	(	-LRB-
cord-014597-66vd2mdu	249	4	Fig	Fig	NNP
cord-014597-66vd2mdu	249	5	.	.	.
cord-014597-66vd2mdu	249	6	1b	1b	CD
cord-014597-66vd2mdu	249	7	)	)	-RRB-
cord-014597-66vd2mdu	249	8	shows	show	VBZ
cord-014597-66vd2mdu	249	9	an	an	DT
cord-014597-66vd2mdu	249	10	example	example	NN
cord-014597-66vd2mdu	249	11	how	how	WRB
cord-014597-66vd2mdu	249	12	this	this	DT
cord-014597-66vd2mdu	249	13	combination	combination	NN
cord-014597-66vd2mdu	249	14	can	can	MD
cord-014597-66vd2mdu	249	15	speed	speed	VB
cord-014597-66vd2mdu	249	16	up	up	RP
cord-014597-66vd2mdu	249	17	process	process	NN
cord-014597-66vd2mdu	249	18	development	development	NN
cord-014597-66vd2mdu	249	19	steps	step	NNS
cord-014597-66vd2mdu	249	20	.	.	.
cord-014597-66vd2mdu	250	1	Main	main	JJ
cord-014597-66vd2mdu	250	2	applications	application	NNS
cord-014597-66vd2mdu	250	3	of	of	IN
cord-014597-66vd2mdu	250	4	the	the	DT
cord-014597-66vd2mdu	250	5	cell	cell	NN
cord-014597-66vd2mdu	250	6	culture	culture	NN
cord-014597-66vd2mdu	250	7	automation	automation	NN
cord-014597-66vd2mdu	250	8	are	be	VBP
cord-014597-66vd2mdu	250	9	for	for	IN
cord-014597-66vd2mdu	250	10	example	example	NN
cord-014597-66vd2mdu	250	11	the	the	DT
cord-014597-66vd2mdu	250	12	identification	identification	NN
cord-014597-66vd2mdu	250	13	of	of	IN
cord-014597-66vd2mdu	250	14	product	product	NN
cord-014597-66vd2mdu	250	15	quality	quality	NN
cord-014597-66vd2mdu	250	16	levers	lever	NNS
cord-014597-66vd2mdu	250	17	and	and	CC
cord-014597-66vd2mdu	250	18	media	medium	NNS
cord-014597-66vd2mdu	250	19	or	or	CC
cord-014597-66vd2mdu	250	20	feed	feed	NN
cord-014597-66vd2mdu	250	21	optimization	optimization	NN
cord-014597-66vd2mdu	250	22	.	.	.
cord-014597-66vd2mdu	251	1	The	the	DT
cord-014597-66vd2mdu	251	2	application	application	NN
cord-014597-66vd2mdu	251	3	of	of	IN
cord-014597-66vd2mdu	251	4	the	the	DT
cord-014597-66vd2mdu	251	5	cell	cell	NN
cord-014597-66vd2mdu	251	6	culture	culture	NN
cord-014597-66vd2mdu	251	7	automation	automation	NN
cord-014597-66vd2mdu	251	8	is	be	VBZ
cord-014597-66vd2mdu	251	9	shown	show	VBN
cord-014597-66vd2mdu	251	10	for	for	IN
cord-014597-66vd2mdu	251	11	two	two	CD
cord-014597-66vd2mdu	251	12	examples	example	NNS
cord-014597-66vd2mdu	251	13	.	.	.
cord-014597-66vd2mdu	252	1	The	the	DT
cord-014597-66vd2mdu	252	2	goal	goal	NN
cord-014597-66vd2mdu	252	3	in	in	IN
cord-014597-66vd2mdu	252	4	the	the	DT
cord-014597-66vd2mdu	252	5	first	first	JJ
cord-014597-66vd2mdu	252	6	application	application	NN
cord-014597-66vd2mdu	252	7	was	be	VBD
cord-014597-66vd2mdu	252	8	to	to	TO
cord-014597-66vd2mdu	252	9	identify	identify	VB
cord-014597-66vd2mdu	252	10	levers	lever	NNS
cord-014597-66vd2mdu	252	11	to	to	TO
cord-014597-66vd2mdu	252	12	reduce	reduce	VB
cord-014597-66vd2mdu	252	13	trisulfides	trisulfide	NNS
cord-014597-66vd2mdu	252	14	.	.	.
cord-014597-66vd2mdu	253	1	By	by	IN
cord-014597-66vd2mdu	253	2	a	a	DT
cord-014597-66vd2mdu	253	3	screening	screening	NN
cord-014597-66vd2mdu	253	4	of	of	IN
cord-014597-66vd2mdu	253	5	39	39	CD
cord-014597-66vd2mdu	253	6	conditions	condition	NNS
cord-014597-66vd2mdu	253	7	in	in	IN
cord-014597-66vd2mdu	253	8	parallel	parallel	NN
cord-014597-66vd2mdu	253	9	(	(	-LRB-
cord-014597-66vd2mdu	253	10	in	in	IN
cord-014597-66vd2mdu	253	11	4-fold	4-fold	CD
cord-014597-66vd2mdu	253	12	replication	replication	NN
cord-014597-66vd2mdu	253	13	,	,	,
cord-014597-66vd2mdu	253	14	158	158	CD
cord-014597-66vd2mdu	253	15	wells	well	NNS
cord-014597-66vd2mdu	253	16	in	in	IN
cord-014597-66vd2mdu	253	17	sum	sum	NN
cord-014597-66vd2mdu	253	18	)	)	-RRB-
cord-014597-66vd2mdu	254	1	the	the	DT
cord-014597-66vd2mdu	254	2	reduction	reduction	NN
cord-014597-66vd2mdu	254	3	of	of	IN
cord-014597-66vd2mdu	254	4	Trisulfides	Trisulfides	NNP
cord-014597-66vd2mdu	254	5	by	by	IN
cord-014597-66vd2mdu	254	6	97.5	97.5	CD
cord-014597-66vd2mdu	254	7	%	%	NN
cord-014597-66vd2mdu	254	8	(	(	-LRB-
cord-014597-66vd2mdu	254	9	normalized	normalize	VBN
cord-014597-66vd2mdu	254	10	to	to	TO
cord-014597-66vd2mdu	254	11	start	start	VB
cord-014597-66vd2mdu	254	12	level	level	NN
cord-014597-66vd2mdu	254	13	)	)	-RRB-
cord-014597-66vd2mdu	254	14	was	be	VBD
cord-014597-66vd2mdu	254	15	possible	possible	JJ
cord-014597-66vd2mdu	254	16	.	.	.
cord-014597-66vd2mdu	255	1	In	in	IN
cord-014597-66vd2mdu	255	2	addition	addition	NN
cord-014597-66vd2mdu	255	3	the	the	DT
cord-014597-66vd2mdu	255	4	levers	lever	NNS
cord-014597-66vd2mdu	255	5	for	for	IN
cord-014597-66vd2mdu	255	6	Trisulfide	Trisulfide	NNP
cord-014597-66vd2mdu	255	7	reduction	reduction	NN
cord-014597-66vd2mdu	255	8	were	be	VBD
cord-014597-66vd2mdu	255	9	identified	identify	VBN
cord-014597-66vd2mdu	255	10	.	.	.
cord-014597-66vd2mdu	256	1	The	the	DT
cord-014597-66vd2mdu	256	2	best	good	JJS
cord-014597-66vd2mdu	256	3	and	and	CC
cord-014597-66vd2mdu	256	4	start	start	VB
cord-014597-66vd2mdu	256	5	conditions	condition	NNS
cord-014597-66vd2mdu	256	6	were	be	VBD
cord-014597-66vd2mdu	256	7	verified	verify	VBN
cord-014597-66vd2mdu	256	8	in	in	IN
cord-014597-66vd2mdu	256	9	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	256	10	scale	scale	NN
cord-014597-66vd2mdu	256	11	(	(	-LRB-
cord-014597-66vd2mdu	256	12	Fig	Fig	NNP
cord-014597-66vd2mdu	256	13	.	.	.
cord-014597-66vd2mdu	256	14	1c	1c	CD
cord-014597-66vd2mdu	256	15	)	)	-RRB-
cord-014597-66vd2mdu	256	16	.	.	.
cord-014597-66vd2mdu	257	1	The	the	DT
cord-014597-66vd2mdu	257	2	goal	goal	NN
cord-014597-66vd2mdu	257	3	in	in	IN
cord-014597-66vd2mdu	257	4	the	the	DT
cord-014597-66vd2mdu	257	5	second	second	JJ
cord-014597-66vd2mdu	257	6	application	application	NN
cord-014597-66vd2mdu	257	7	was	be	VBD
cord-014597-66vd2mdu	257	8	to	to	TO
cord-014597-66vd2mdu	257	9	increase	increase	VB
cord-014597-66vd2mdu	257	10	product	product	NN
cord-014597-66vd2mdu	257	11	concentration	concentration	NN
cord-014597-66vd2mdu	257	12	without	without	IN
cord-014597-66vd2mdu	257	13	an	an	DT
cord-014597-66vd2mdu	257	14	impact	impact	NN
cord-014597-66vd2mdu	257	15	on	on	IN
cord-014597-66vd2mdu	257	16	product	product	NN
cord-014597-66vd2mdu	257	17	quality	quality	NN
cord-014597-66vd2mdu	257	18	.	.	.
cord-014597-66vd2mdu	258	1	By	by	IN
cord-014597-66vd2mdu	258	2	a	a	DT
cord-014597-66vd2mdu	258	3	screening	screening	NN
cord-014597-66vd2mdu	258	4	of	of	IN
cord-014597-66vd2mdu	258	5	54	54	CD
cord-014597-66vd2mdu	258	6	conditions	condition	NNS
cord-014597-66vd2mdu	258	7	in	in	IN
cord-014597-66vd2mdu	258	8	parallel	parallel	NN
cord-014597-66vd2mdu	258	9	(	(	-LRB-
cord-014597-66vd2mdu	258	10	in	in	IN
cord-014597-66vd2mdu	258	11	4-fold	4-fold	CD
cord-014597-66vd2mdu	258	12	replication	replication	NN
cord-014597-66vd2mdu	258	13	,	,	,
cord-014597-66vd2mdu	258	14	216	216	CD
cord-014597-66vd2mdu	258	15	wells	well	NNS
cord-014597-66vd2mdu	258	16	in	in	IN
cord-014597-66vd2mdu	258	17	sum	sum	NN
cord-014597-66vd2mdu	258	18	)	)	-RRB-
cord-014597-66vd2mdu	259	1	the	the	DT
cord-014597-66vd2mdu	259	2	increase	increase	NN
cord-014597-66vd2mdu	259	3	of	of	IN
cord-014597-66vd2mdu	259	4	titer	titer	NN
cord-014597-66vd2mdu	259	5	from	from	IN
cord-014597-66vd2mdu	259	6	1.5	1.5	CD
cord-014597-66vd2mdu	259	7	g	g	NN
cord-014597-66vd2mdu	259	8	/	/	SYM
cord-014597-66vd2mdu	259	9	L	l	NN
cord-014597-66vd2mdu	259	10	to	to	IN
cord-014597-66vd2mdu	259	11	3.7	3.7	CD
cord-014597-66vd2mdu	259	12	g	g	NN
cord-014597-66vd2mdu	259	13	/	/	SYM
cord-014597-66vd2mdu	259	14	L	l	NN
cord-014597-66vd2mdu	259	15	(	(	-LRB-
cord-014597-66vd2mdu	259	16	>	>	XX
cord-014597-66vd2mdu	260	1	factor	factor	NN
cord-014597-66vd2mdu	260	2	2	2	CD
cord-014597-66vd2mdu	260	3	)	)	-RRB-
cord-014597-66vd2mdu	260	4	was	be	VBD
cord-014597-66vd2mdu	260	5	possible	possible	JJ
cord-014597-66vd2mdu	260	6	by	by	IN
cord-014597-66vd2mdu	260	7	media	medium	NNS
cord-014597-66vd2mdu	260	8	platform	platform	NN
cord-014597-66vd2mdu	260	9	change	change	NN
cord-014597-66vd2mdu	260	10	and	and	CC
cord-014597-66vd2mdu	260	11	media	medium	NNS
cord-014597-66vd2mdu	260	12	optimization	optimization	NN
cord-014597-66vd2mdu	260	13	.	.	.
cord-014597-66vd2mdu	261	1	An	an	DT
cord-014597-66vd2mdu	261	2	impact	impact	NN
cord-014597-66vd2mdu	261	3	on	on	IN
cord-014597-66vd2mdu	261	4	product	product	NN
cord-014597-66vd2mdu	261	5	quality	quality	NN
cord-014597-66vd2mdu	261	6	could	could	MD
cord-014597-66vd2mdu	261	7	not	not	RB
cord-014597-66vd2mdu	261	8	been	be	VBN
cord-014597-66vd2mdu	261	9	shown	show	VBN
cord-014597-66vd2mdu	261	10	.	.	.
cord-014597-66vd2mdu	262	1	The	the	DT
cord-014597-66vd2mdu	262	2	best	good	JJS
cord-014597-66vd2mdu	262	3	conditions	condition	NNS
cord-014597-66vd2mdu	262	4	were	be	VBD
cord-014597-66vd2mdu	262	5	also	also	RB
cord-014597-66vd2mdu	262	6	verified	verify	VBN
cord-014597-66vd2mdu	262	7	in	in	IN
cord-014597-66vd2mdu	262	8	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	262	9	scale	scale	NN
cord-014597-66vd2mdu	262	10	(	(	-LRB-
cord-014597-66vd2mdu	262	11	Fig	Fig	NNP
cord-014597-66vd2mdu	262	12	.	.	.
cord-014597-66vd2mdu	262	13	1d	1d	CD
cord-014597-66vd2mdu	262	14	)	)	-RRB-
cord-014597-66vd2mdu	262	15	.	.	.
cord-014597-66vd2mdu	263	1	The	the	DT
cord-014597-66vd2mdu	263	2	benefits	benefit	NNS
cord-014597-66vd2mdu	263	3	of	of	IN
cord-014597-66vd2mdu	263	4	using	use	VBG
cord-014597-66vd2mdu	263	5	cell	cell	NN
cord-014597-66vd2mdu	263	6	culture	culture	NN
cord-014597-66vd2mdu	263	7	automation	automation	NN
cord-014597-66vd2mdu	263	8	in	in	IN
cord-014597-66vd2mdu	263	9	late	late	JJ
cord-014597-66vd2mdu	263	10	stage	stage	NN
cord-014597-66vd2mdu	263	11	process	process	NN
cord-014597-66vd2mdu	263	12	development	development	NN
cord-014597-66vd2mdu	263	13	were	be	VBD
cord-014597-66vd2mdu	263	14	shown	show	VBN
cord-014597-66vd2mdu	263	15	based	base	VBN
cord-014597-66vd2mdu	263	16	on	on	IN
cord-014597-66vd2mdu	263	17	two	two	CD
cord-014597-66vd2mdu	263	18	examples	example	NNS
cord-014597-66vd2mdu	263	19	of	of	IN
cord-014597-66vd2mdu	263	20	current	current	JJ
cord-014597-66vd2mdu	263	21	applications	application	NNS
cord-014597-66vd2mdu	263	22	.	.	.
cord-014597-66vd2mdu	264	1	For	for	IN
cord-014597-66vd2mdu	264	2	this	this	DT
cord-014597-66vd2mdu	264	3	purpose	purpose	NN
cord-014597-66vd2mdu	264	4	the	the	DT
cord-014597-66vd2mdu	264	5	experimental	experimental	JJ
cord-014597-66vd2mdu	264	6	results	result	NNS
cord-014597-66vd2mdu	264	7	of	of	IN
cord-014597-66vd2mdu	264	8	the	the	DT
cord-014597-66vd2mdu	264	9	development	development	NN
cord-014597-66vd2mdu	264	10	work	work	NN
cord-014597-66vd2mdu	264	11	of	of	IN
cord-014597-66vd2mdu	264	12	two	two	CD
cord-014597-66vd2mdu	264	13	late	late	JJ
cord-014597-66vd2mdu	264	14	state	state	NN
cord-014597-66vd2mdu	264	15	projects	project	NNS
cord-014597-66vd2mdu	264	16	using	use	VBG
cord-014597-66vd2mdu	264	17	the	the	DT
cord-014597-66vd2mdu	264	18	in	in	IN
cord-014597-66vd2mdu	264	19	-	-	HYPH
cord-014597-66vd2mdu	264	20	house	house	NN
cord-014597-66vd2mdu	264	21	developed	develop	VBD
cord-014597-66vd2mdu	264	22	automated	automated	JJ
cord-014597-66vd2mdu	264	23	cell	cell	NN
cord-014597-66vd2mdu	264	24	culture	culture	NN
cord-014597-66vd2mdu	264	25	system	system	NN
cord-014597-66vd2mdu	264	26	were	be	VBD
cord-014597-66vd2mdu	264	27	shown	show	VBN
cord-014597-66vd2mdu	264	28	.	.	.
cord-014597-66vd2mdu	265	1	The	the	DT
cord-014597-66vd2mdu	265	2	first	first	JJ
cord-014597-66vd2mdu	265	3	example	example	NN
cord-014597-66vd2mdu	265	4	shows	show	VBZ
cord-014597-66vd2mdu	265	5	the	the	DT
cord-014597-66vd2mdu	265	6	capability	capability	NN
cord-014597-66vd2mdu	265	7	of	of	IN
cord-014597-66vd2mdu	265	8	the	the	DT
cord-014597-66vd2mdu	265	9	automated	automate	VBN
cord-014597-66vd2mdu	265	10	cell	cell	NN
cord-014597-66vd2mdu	265	11	culture	culture	NN
cord-014597-66vd2mdu	265	12	system	system	NN
cord-014597-66vd2mdu	265	13	by	by	IN
cord-014597-66vd2mdu	265	14	reducing	reduce	VBG
cord-014597-66vd2mdu	265	15	trisulfides	trisulfide	NNS
cord-014597-66vd2mdu	265	16	significantly	significantly	RB
cord-014597-66vd2mdu	265	17	in	in	IN
cord-014597-66vd2mdu	265	18	just	just	RB
cord-014597-66vd2mdu	265	19	one	one	CD
cord-014597-66vd2mdu	265	20	experiment	experiment	NN
cord-014597-66vd2mdu	265	21	.	.	.
cord-014597-66vd2mdu	266	1	For	for	IN
cord-014597-66vd2mdu	266	2	the	the	DT
cord-014597-66vd2mdu	266	3	other	other	JJ
cord-014597-66vd2mdu	266	4	project	project	NN
cord-014597-66vd2mdu	266	5	the	the	DT
cord-014597-66vd2mdu	266	6	final	final	JJ
cord-014597-66vd2mdu	266	7	product	product	NN
cord-014597-66vd2mdu	266	8	concentration	concentration	NN
cord-014597-66vd2mdu	266	9	could	could	MD
cord-014597-66vd2mdu	266	10	be	be	VB
cord-014597-66vd2mdu	266	11	increased	increase	VBN
cord-014597-66vd2mdu	266	12	by	by	IN
cord-014597-66vd2mdu	266	13	factor	factor	NN
cord-014597-66vd2mdu	266	14	2.5	2.5	CD
cord-014597-66vd2mdu	266	15	by	by	IN
cord-014597-66vd2mdu	266	16	a	a	DT
cord-014597-66vd2mdu	266	17	media	medium	NNS
cord-014597-66vd2mdu	266	18	screening	screening	NN
cord-014597-66vd2mdu	266	19	and	and	CC
cord-014597-66vd2mdu	266	20	changing	change	VBG
cord-014597-66vd2mdu	266	21	to	to	IN
cord-014597-66vd2mdu	266	22	the	the	DT
cord-014597-66vd2mdu	266	23	in	in	IN
cord-014597-66vd2mdu	266	24	-	-	HYPH
cord-014597-66vd2mdu	266	25	house	house	NN
cord-014597-66vd2mdu	266	26	media	medium	NNS
cord-014597-66vd2mdu	266	27	platform	platform	NN
cord-014597-66vd2mdu	266	28	.	.	.
cord-014597-66vd2mdu	267	1	These	these	DT
cord-014597-66vd2mdu	267	2	two	two	CD
cord-014597-66vd2mdu	267	3	examples	example	NNS
cord-014597-66vd2mdu	267	4	show	show	VBP
cord-014597-66vd2mdu	267	5	the	the	DT
cord-014597-66vd2mdu	267	6	potential	potential	NN
cord-014597-66vd2mdu	267	7	of	of	IN
cord-014597-66vd2mdu	267	8	cell	cell	NN
cord-014597-66vd2mdu	267	9	culture	culture	NN
cord-014597-66vd2mdu	267	10	automation	automation	NN
cord-014597-66vd2mdu	267	11	as	as	IN
cord-014597-66vd2mdu	267	12	a	a	DT
cord-014597-66vd2mdu	267	13	routine	routine	JJ
cord-014597-66vd2mdu	267	14	tool	tool	NN
cord-014597-66vd2mdu	267	15	in	in	IN
cord-014597-66vd2mdu	267	16	process	process	NN
cord-014597-66vd2mdu	267	17	development	development	NN
cord-014597-66vd2mdu	267	18	.	.	.
cord-014597-66vd2mdu	268	1	The	the	DT
cord-014597-66vd2mdu	268	2	cell	cell	NN
cord-014597-66vd2mdu	268	3	line	line	NN
cord-014597-66vd2mdu	268	4	development	development	NN
cord-014597-66vd2mdu	268	5	process	process	NN
cord-014597-66vd2mdu	268	6	has	have	VBZ
cord-014597-66vd2mdu	268	7	become	become	VBN
cord-014597-66vd2mdu	268	8	faster	fast	RBR
cord-014597-66vd2mdu	268	9	and	and	CC
cord-014597-66vd2mdu	268	10	is	be	VBZ
cord-014597-66vd2mdu	268	11	simultaneously	simultaneously	RB
cord-014597-66vd2mdu	268	12	generating	generate	VBG
cord-014597-66vd2mdu	268	13	more	more	JJR
cord-014597-66vd2mdu	268	14	clone	clone	NN
cord-014597-66vd2mdu	268	15	-	-	HYPH
cord-014597-66vd2mdu	268	16	and	and	CC
cord-014597-66vd2mdu	268	17	product	product	NN
cord-014597-66vd2mdu	268	18	-	-	HYPH
cord-014597-66vd2mdu	268	19	related	relate	VBN
cord-014597-66vd2mdu	268	20	analytical	analytical	JJ
cord-014597-66vd2mdu	268	21	data	datum	NNS
cord-014597-66vd2mdu	268	22	.	.	.
cord-014597-66vd2mdu	269	1	In	in	IN
cord-014597-66vd2mdu	269	2	order	order	NN
cord-014597-66vd2mdu	269	3	to	to	TO
cord-014597-66vd2mdu	269	4	select	select	VB
cord-014597-66vd2mdu	269	5	the	the	DT
cord-014597-66vd2mdu	269	6	best	good	JJS
cord-014597-66vd2mdu	269	7	producer	producer	NN
cord-014597-66vd2mdu	269	8	cell	cell	NN
cord-014597-66vd2mdu	269	9	line	line	NN
cord-014597-66vd2mdu	269	10	,	,	,
cord-014597-66vd2mdu	269	11	extremely	extremely	RB
cord-014597-66vd2mdu	269	12	heterogeneous	heterogeneous	JJ
cord-014597-66vd2mdu	269	13	data	datum	NNS
cord-014597-66vd2mdu	269	14	types	type	NNS
cord-014597-66vd2mdu	269	15	need	need	VBP
cord-014597-66vd2mdu	269	16	to	to	TO
cord-014597-66vd2mdu	269	17	be	be	VB
cord-014597-66vd2mdu	269	18	systematically	systematically	RB
cord-014597-66vd2mdu	269	19	compared	compare	VBN
cord-014597-66vd2mdu	269	20	.	.	.
cord-014597-66vd2mdu	270	1	The	the	DT
cord-014597-66vd2mdu	270	2	timely	timely	JJ
cord-014597-66vd2mdu	270	3	availability	availability	NN
cord-014597-66vd2mdu	270	4	of	of	IN
cord-014597-66vd2mdu	270	5	all	all	DT
cord-014597-66vd2mdu	270	6	data	datum	NNS
cord-014597-66vd2mdu	270	7	needed	need	VBN
cord-014597-66vd2mdu	270	8	to	to	TO
cord-014597-66vd2mdu	270	9	decide	decide	VB
cord-014597-66vd2mdu	270	10	which	which	WDT
cord-014597-66vd2mdu	270	11	cell	cell	NN
cord-014597-66vd2mdu	270	12	line	line	NN
cord-014597-66vd2mdu	270	13	to	to	TO
cord-014597-66vd2mdu	270	14	pursue	pursue	VB
cord-014597-66vd2mdu	270	15	has	have	VBZ
cord-014597-66vd2mdu	270	16	become	become	VBN
cord-014597-66vd2mdu	270	17	a	a	DT
cord-014597-66vd2mdu	270	18	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	270	19	in	in	IN
cord-014597-66vd2mdu	270	20	the	the	DT
cord-014597-66vd2mdu	270	21	cell	cell	NN
cord-014597-66vd2mdu	270	22	line	line	NN
cord-014597-66vd2mdu	270	23	development	development	NN
cord-014597-66vd2mdu	270	24	workflow	workflow	NN
cord-014597-66vd2mdu	270	25	.	.	.
cord-014597-66vd2mdu	271	1	To	to	TO
cord-014597-66vd2mdu	271	2	ensure	ensure	VB
cord-014597-66vd2mdu	271	3	sound	sound	JJ
cord-014597-66vd2mdu	271	4	decision	decision	NN
cord-014597-66vd2mdu	271	5	making	making	NN
cord-014597-66vd2mdu	271	6	,	,	,
cord-014597-66vd2mdu	271	7	new	new	JJ
cord-014597-66vd2mdu	271	8	integrated	integrated	JJ
cord-014597-66vd2mdu	271	9	workflow	workflow	NN
cord-014597-66vd2mdu	271	10	support	support	NN
cord-014597-66vd2mdu	271	11	and	and	CC
cord-014597-66vd2mdu	271	12	data	data	NN
cord-014597-66vd2mdu	271	13	analysis	analysis	NN
cord-014597-66vd2mdu	271	14	methods	method	NNS
cord-014597-66vd2mdu	271	15	are	be	VBP
cord-014597-66vd2mdu	271	16	needed	need	VBN
cord-014597-66vd2mdu	271	17	.	.	.
cord-014597-66vd2mdu	272	1	We	-PRON-	PRP
cord-014597-66vd2mdu	272	2	have	have	VBP
cord-014597-66vd2mdu	272	3	developed	develop	VBN
cord-014597-66vd2mdu	272	4	a	a	DT
cord-014597-66vd2mdu	272	5	new	new	JJ
cord-014597-66vd2mdu	272	6	end	end	NN
cord-014597-66vd2mdu	272	7	-	-	HYPH
cord-014597-66vd2mdu	272	8	to	to	IN
cord-014597-66vd2mdu	272	9	-	-	HYPH
cord-014597-66vd2mdu	272	10	end	end	NN
cord-014597-66vd2mdu	272	11	platform	platform	NN
cord-014597-66vd2mdu	272	12	for	for	IN
cord-014597-66vd2mdu	272	13	bioprocess	bioprocess	NNP
cord-014597-66vd2mdu	272	14	development	development	NNP
cord-014597-66vd2mdu	272	15	,	,	,
cord-014597-66vd2mdu	272	16	which	which	WDT
cord-014597-66vd2mdu	272	17	includes	include	VBZ
cord-014597-66vd2mdu	272	18	a	a	DT
cord-014597-66vd2mdu	272	19	cell	cell	NN
cord-014597-66vd2mdu	272	20	line	line	NN
cord-014597-66vd2mdu	272	21	development	development	NN
cord-014597-66vd2mdu	272	22	workflow	workflow	NN
cord-014597-66vd2mdu	272	23	system	system	NN
cord-014597-66vd2mdu	272	24	supporting	support	VBG
cord-014597-66vd2mdu	272	25	seeding	seeding	NN
cord-014597-66vd2mdu	272	26	,	,	,
cord-014597-66vd2mdu	272	27	selection	selection	NN
cord-014597-66vd2mdu	272	28	,	,	,
cord-014597-66vd2mdu	272	29	passaging	passaging	NN
cord-014597-66vd2mdu	272	30	,	,	,
cord-014597-66vd2mdu	272	31	analyzing	analyze	VBG
cord-014597-66vd2mdu	272	32	,	,	,
cord-014597-66vd2mdu	272	33	cryo	cryo	NN
cord-014597-66vd2mdu	272	34	-	-	HYPH
cord-014597-66vd2mdu	272	35	conservation	conservation	NN
cord-014597-66vd2mdu	272	36	,	,	,
cord-014597-66vd2mdu	272	37	and	and	CC
cord-014597-66vd2mdu	272	38	processing	processing	NN
cord-014597-66vd2mdu	272	39	in	in	IN
cord-014597-66vd2mdu	272	40	(	(	-LRB-
cord-014597-66vd2mdu	272	41	micro-	micro-	NN
cord-014597-66vd2mdu	272	42	)	)	-RRB-
cord-014597-66vd2mdu	272	43	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	272	44	.	.	.
cord-014597-66vd2mdu	273	1	This	this	DT
cord-014597-66vd2mdu	273	2	platform	platform	NN
cord-014597-66vd2mdu	273	3	,	,	,
cord-014597-66vd2mdu	273	4	Genedata	Genedata	NNP
cord-014597-66vd2mdu	273	5	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	273	6	™	™	NN
cord-014597-66vd2mdu	273	7	,	,	,
cord-014597-66vd2mdu	273	8	enables	enable	VBZ
cord-014597-66vd2mdu	273	9	partially	partially	RB
cord-014597-66vd2mdu	273	10	or	or	CC
cord-014597-66vd2mdu	273	11	fully	fully	RB
cord-014597-66vd2mdu	273	12	automated	automate	VBN
cord-014597-66vd2mdu	273	13	cell	cell	NN
cord-014597-66vd2mdu	273	14	line	line	NN
cord-014597-66vd2mdu	273	15	selection	selection	NN
cord-014597-66vd2mdu	273	16	and	and	CC
cord-014597-66vd2mdu	273	17	assessment	assessment	NN
cord-014597-66vd2mdu	273	18	processes	process	NNS
cord-014597-66vd2mdu	273	19	,	,	,
cord-014597-66vd2mdu	273	20	and	and	CC
cord-014597-66vd2mdu	273	21	it	-PRON-	PRP
cord-014597-66vd2mdu	273	22	increases	increase	VBZ
cord-014597-66vd2mdu	273	23	process	process	NN
cord-014597-66vd2mdu	273	24	efficiency	efficiency	NN
cord-014597-66vd2mdu	273	25	and	and	CC
cord-014597-66vd2mdu	273	26	quality	quality	NN
cord-014597-66vd2mdu	273	27	.	.	.
cord-014597-66vd2mdu	274	1	The	the	DT
cord-014597-66vd2mdu	274	2	system	system	NN
cord-014597-66vd2mdu	274	3	tracks	track	VBZ
cord-014597-66vd2mdu	274	4	the	the	DT
cord-014597-66vd2mdu	274	5	full	full	JJ
cord-014597-66vd2mdu	274	6	history	history	NN
cord-014597-66vd2mdu	274	7	of	of	IN
cord-014597-66vd2mdu	274	8	all	all	DT
cord-014597-66vd2mdu	274	9	clones	clone	NNS
cord-014597-66vd2mdu	274	10	-from	-from	IN
cord-014597-66vd2mdu	274	11	initial	initial	JJ
cord-014597-66vd2mdu	274	12	transfection	transfection	NN
cord-014597-66vd2mdu	274	13	all	all	PDT
cord-014597-66vd2mdu	274	14	the	the	DT
cord-014597-66vd2mdu	274	15	way	way	NN
cord-014597-66vd2mdu	274	16	to	to	IN
cord-014597-66vd2mdu	274	17	their	-PRON-	PRP$
cord-014597-66vd2mdu	274	18	evaluation	evaluation	NN
cord-014597-66vd2mdu	274	19	in	in	IN
cord-014597-66vd2mdu	274	20	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	274	21	runs	run	NNS
cord-014597-66vd2mdu	274	22	-and	-and	POS
cord-014597-66vd2mdu	274	23	combines	combine	VBZ
cord-014597-66vd2mdu	274	24	this	this	DT
cord-014597-66vd2mdu	274	25	information	information	NN
cord-014597-66vd2mdu	274	26	with	with	IN
cord-014597-66vd2mdu	274	27	analytics	analytic	NNS
cord-014597-66vd2mdu	274	28	data	datum	NNS
cord-014597-66vd2mdu	274	29	on	on	IN
cord-014597-66vd2mdu	274	30	molecules	molecule	NNS
cord-014597-66vd2mdu	274	31	,	,	,
cord-014597-66vd2mdu	274	32	clones	clone	NNS
cord-014597-66vd2mdu	274	33	,	,	,
cord-014597-66vd2mdu	274	34	and	and	CC
cord-014597-66vd2mdu	274	35	product	product	NN
cord-014597-66vd2mdu	274	36	quality	quality	NN
cord-014597-66vd2mdu	274	37	.	.	.
cord-014597-66vd2mdu	275	1	It	-PRON-	PRP
cord-014597-66vd2mdu	275	2	can	can	MD
cord-014597-66vd2mdu	275	3	directly	directly	RB
cord-014597-66vd2mdu	275	4	integrate	integrate	VB
cord-014597-66vd2mdu	275	5	with	with	IN
cord-014597-66vd2mdu	275	6	all	all	DT
cord-014597-66vd2mdu	275	7	instruments	instrument	NNS
cord-014597-66vd2mdu	275	8	,	,	,
cord-014597-66vd2mdu	275	9	such	such	JJ
cord-014597-66vd2mdu	275	10	as	as	IN
cord-014597-66vd2mdu	275	11	pipetting	pipetting	JJ
cord-014597-66vd2mdu	275	12	robots	robot	NNS
cord-014597-66vd2mdu	275	13	,	,	,
cord-014597-66vd2mdu	275	14	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	275	15	,	,	,
cord-014597-66vd2mdu	275	16	and	and	CC
cord-014597-66vd2mdu	275	17	bioanalyzers	bioanalyzer	NNS
cord-014597-66vd2mdu	275	18	.	.	.
cord-014597-66vd2mdu	276	1	The	the	DT
cord-014597-66vd2mdu	276	2	system	system	NN
cord-014597-66vd2mdu	276	3	is	be	VBZ
cord-014597-66vd2mdu	276	4	designed	design	VBN
cord-014597-66vd2mdu	276	5	for	for	IN
cord-014597-66vd2mdu	276	6	a	a	DT
cord-014597-66vd2mdu	276	7	wide	wide	JJ
cord-014597-66vd2mdu	276	8	range	range	NN
cord-014597-66vd2mdu	276	9	of	of	IN
cord-014597-66vd2mdu	276	10	.	.	.
cord-014597-66vd2mdu	277	1	a	a	DT
cord-014597-66vd2mdu	277	2	Schematic	schematic	JJ
cord-014597-66vd2mdu	277	3	illustration	illustration	NN
cord-014597-66vd2mdu	277	4	of	of	IN
cord-014597-66vd2mdu	277	5	the	the	DT
cord-014597-66vd2mdu	277	6	automated	automate	VBN
cord-014597-66vd2mdu	277	7	cell	cell	NN
cord-014597-66vd2mdu	277	8	culture	culture	NN
cord-014597-66vd2mdu	277	9	system	system	NN
cord-014597-66vd2mdu	277	10	.	.	.
cord-014597-66vd2mdu	278	1	Only	only	RB
cord-014597-66vd2mdu	278	2	the	the	DT
cord-014597-66vd2mdu	278	3	core	core	NN
cord-014597-66vd2mdu	278	4	system	system	NN
cord-014597-66vd2mdu	278	5	is	be	VBZ
cord-014597-66vd2mdu	278	6	shown	show	VBN
cord-014597-66vd2mdu	278	7	with	with	IN
cord-014597-66vd2mdu	278	8	a	a	DT
cord-014597-66vd2mdu	278	9	robotic	robotic	JJ
cord-014597-66vd2mdu	278	10	plate	plate	NN
cord-014597-66vd2mdu	278	11	handler	handler	NN
cord-014597-66vd2mdu	278	12	as	as	IN
cord-014597-66vd2mdu	278	13	key	key	JJ
cord-014597-66vd2mdu	278	14	device	device	NN
cord-014597-66vd2mdu	278	15	connecting	connect	VBG
cord-014597-66vd2mdu	278	16	cultivation	cultivation	NN
cord-014597-66vd2mdu	278	17	,	,	,
cord-014597-66vd2mdu	278	18	processing	processing	NN
cord-014597-66vd2mdu	278	19	and	and	CC
cord-014597-66vd2mdu	278	20	analytical	analytical	JJ
cord-014597-66vd2mdu	278	21	parts	part	NNS
cord-014597-66vd2mdu	278	22	.	.	.
cord-014597-66vd2mdu	279	1	b	b	LS
cord-014597-66vd2mdu	279	2	Illustration	illustration	NN
cord-014597-66vd2mdu	279	3	of	of	IN
cord-014597-66vd2mdu	279	4	an	an	DT
cord-014597-66vd2mdu	279	5	example	example	NN
cord-014597-66vd2mdu	279	6	how	how	WRB
cord-014597-66vd2mdu	279	7	cell	cell	NN
cord-014597-66vd2mdu	279	8	culture	culture	NN
cord-014597-66vd2mdu	279	9	automation	automation	NN
cord-014597-66vd2mdu	279	10	can	can	MD
cord-014597-66vd2mdu	279	11	speed	speed	VB
cord-014597-66vd2mdu	279	12	up	up	RP
cord-014597-66vd2mdu	279	13	process	process	NN
cord-014597-66vd2mdu	279	14	development	development	NN
cord-014597-66vd2mdu	279	15	steps	step	NNS
cord-014597-66vd2mdu	279	16	.	.	.
cord-014597-66vd2mdu	280	1	c	c	NNP
cord-014597-66vd2mdu	280	2	Application	Application	NNP
cord-014597-66vd2mdu	280	3	in	in	IN
cord-014597-66vd2mdu	280	4	the	the	DT
cord-014597-66vd2mdu	280	5	identification	identification	NN
cord-014597-66vd2mdu	280	6	of	of	IN
cord-014597-66vd2mdu	280	7	product	product	NN
cord-014597-66vd2mdu	280	8	quality	quality	NN
cord-014597-66vd2mdu	280	9	levers	lever	NNS
cord-014597-66vd2mdu	280	10	.	.	.
cord-014597-66vd2mdu	281	1	d	d	NNP
cord-014597-66vd2mdu	281	2	Application	Application	NNP
cord-014597-66vd2mdu	281	3	in	in	IN
cord-014597-66vd2mdu	281	4	titer	titer	NN
cord-014597-66vd2mdu	281	5	optimization	optimization	NN
cord-014597-66vd2mdu	281	6	biologic	biologic	NN
cord-014597-66vd2mdu	281	7	molecules	molecule	NNS
cord-014597-66vd2mdu	281	8	,	,	,
cord-014597-66vd2mdu	281	9	including	include	VBG
cord-014597-66vd2mdu	281	10	antibodies	antibody	NNS
cord-014597-66vd2mdu	281	11	(	(	-LRB-
cord-014597-66vd2mdu	281	12	IgGs	IgGs	NNP
cord-014597-66vd2mdu	281	13	,	,	,
cord-014597-66vd2mdu	281	14	novel	novel	JJ
cord-014597-66vd2mdu	281	15	formats	format	NNS
cord-014597-66vd2mdu	281	16	)	)	-RRB-
cord-014597-66vd2mdu	281	17	and	and	CC
cord-014597-66vd2mdu	281	18	other	other	JJ
cord-014597-66vd2mdu	281	19	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	281	20	proteins	protein	NNS
cord-014597-66vd2mdu	281	21	(	(	-LRB-
cord-014597-66vd2mdu	281	22	e.g.	e.g.	RB
cord-014597-66vd2mdu	281	23	,	,	,
cord-014597-66vd2mdu	281	24	fusion	fusion	NN
cord-014597-66vd2mdu	281	25	proteins	protein	NNS
cord-014597-66vd2mdu	281	26	)	)	-RRB-
cord-014597-66vd2mdu	281	27	.	.	.
cord-014597-66vd2mdu	282	1	Highlighted	highlight	VBN
cord-014597-66vd2mdu	282	2	use	use	NN
cord-014597-66vd2mdu	282	3	cases	case	NNS
cord-014597-66vd2mdu	282	4	describe	describe	VBP
cord-014597-66vd2mdu	282	5	the	the	DT
cord-014597-66vd2mdu	282	6	identification	identification	NN
cord-014597-66vd2mdu	282	7	of	of	IN
cord-014597-66vd2mdu	282	8	top	top	JJ
cord-014597-66vd2mdu	282	9	producer	producer	NN
cord-014597-66vd2mdu	282	10	cell	cell	NN
cord-014597-66vd2mdu	282	11	lines	line	NNS
cord-014597-66vd2mdu	282	12	,	,	,
cord-014597-66vd2mdu	282	13	decision	decision	NN
cord-014597-66vd2mdu	282	14	making	make	VBG
cord-014597-66vd2mdu	282	15	support	support	NN
cord-014597-66vd2mdu	282	16	,	,	,
cord-014597-66vd2mdu	282	17	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	282	18	data	datum	NNS
cord-014597-66vd2mdu	282	19	management	management	NN
cord-014597-66vd2mdu	282	20	,	,	,
cord-014597-66vd2mdu	282	21	and	and	CC
cord-014597-66vd2mdu	282	22	full	full	JJ
cord-014597-66vd2mdu	282	23	clone	clone	NN
cord-014597-66vd2mdu	282	24	history	history	NN
cord-014597-66vd2mdu	282	25	report	report	NN
cord-014597-66vd2mdu	282	26	documentation	documentation	NN
cord-014597-66vd2mdu	282	27	(	(	-LRB-
cord-014597-66vd2mdu	282	28	Fig	Fig	NNP
cord-014597-66vd2mdu	282	29	.	.	.
cord-014597-66vd2mdu	282	30	1	1	CD
cord-014597-66vd2mdu	282	31	)	)	-RRB-
cord-014597-66vd2mdu	282	32	.	.	.
cord-014597-66vd2mdu	283	1	Genedata	Genedata	NNP
cord-014597-66vd2mdu	283	2	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	283	3	,	,	,
cord-014597-66vd2mdu	283	4	which	which	WDT
cord-014597-66vd2mdu	283	5	was	be	VBD
cord-014597-66vd2mdu	283	6	developed	develop	VBN
cord-014597-66vd2mdu	283	7	in	in	IN
cord-014597-66vd2mdu	283	8	collaboration	collaboration	NN
cord-014597-66vd2mdu	283	9	with	with	IN
cord-014597-66vd2mdu	283	10	top	top	JJ
cord-014597-66vd2mdu	283	11	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	283	12	companies	company	NNS
cord-014597-66vd2mdu	283	13	,	,	,
cord-014597-66vd2mdu	283	14	can	can	MD
cord-014597-66vd2mdu	283	15	flexibly	flexibly	RB
cord-014597-66vd2mdu	283	16	support	support	VB
cord-014597-66vd2mdu	283	17	various	various	JJ
cord-014597-66vd2mdu	283	18	(	(	-LRB-
cord-014597-66vd2mdu	283	19	non	non	JJ
cord-014597-66vd2mdu	283	20	-	-	JJ
cord-014597-66vd2mdu	283	21	linear	linear	JJ
cord-014597-66vd2mdu	283	22	)	)	-RRB-
cord-014597-66vd2mdu	283	23	workflows	workflow	NNS
cord-014597-66vd2mdu	283	24	and	and	CC
cord-014597-66vd2mdu	283	25	structure	structure	NN
cord-014597-66vd2mdu	283	26	the	the	DT
cord-014597-66vd2mdu	283	27	collected	collect	VBN
cord-014597-66vd2mdu	283	28	information	information	NN
cord-014597-66vd2mdu	283	29	in	in	IN
cord-014597-66vd2mdu	283	30	a	a	DT
cord-014597-66vd2mdu	283	31	way	way	NN
cord-014597-66vd2mdu	283	32	that	that	WDT
cord-014597-66vd2mdu	283	33	fosters	foster	VBZ
cord-014597-66vd2mdu	283	34	collaboration	collaboration	NN
cord-014597-66vd2mdu	283	35	across	across	IN
cord-014597-66vd2mdu	283	36	an	an	DT
cord-014597-66vd2mdu	283	37	organization	organization	NN
cord-014597-66vd2mdu	283	38	.	.	.
cord-014597-66vd2mdu	284	1	While	while	IN
cord-014597-66vd2mdu	284	2	increasing	increase	VBG
cord-014597-66vd2mdu	284	3	throughput	throughput	NN
cord-014597-66vd2mdu	284	4	is	be	VBZ
cord-014597-66vd2mdu	284	5	crucial	crucial	JJ
cord-014597-66vd2mdu	284	6	to	to	TO
cord-014597-66vd2mdu	284	7	ensure	ensure	VB
cord-014597-66vd2mdu	284	8	the	the	DT
cord-014597-66vd2mdu	284	9	timely	timely	JJ
cord-014597-66vd2mdu	284	10	availability	availability	NN
cord-014597-66vd2mdu	284	11	of	of	IN
cord-014597-66vd2mdu	284	12	optimal	optimal	JJ
cord-014597-66vd2mdu	284	13	producer	producer	NN
cord-014597-66vd2mdu	284	14	cell	cell	NN
cord-014597-66vd2mdu	284	15	lines	line	NNS
cord-014597-66vd2mdu	284	16	,	,	,
cord-014597-66vd2mdu	284	17	high	high	JJ
cord-014597-66vd2mdu	284	18	-	-	HYPH
cord-014597-66vd2mdu	284	19	throughput	throughput	NN
cord-014597-66vd2mdu	284	20	is	be	VBZ
cord-014597-66vd2mdu	284	21	only	only	RB
cord-014597-66vd2mdu	284	22	possible	possible	JJ
cord-014597-66vd2mdu	284	23	when	when	WRB
cord-014597-66vd2mdu	284	24	automated	automate	VBN
cord-014597-66vd2mdu	284	25	processes	process	NNS
cord-014597-66vd2mdu	284	26	in	in	IN
cord-014597-66vd2mdu	284	27	the	the	DT
cord-014597-66vd2mdu	284	28	laboratory	laboratory	NN
cord-014597-66vd2mdu	284	29	and	and	CC
cord-014597-66vd2mdu	284	30	the	the	DT
cord-014597-66vd2mdu	284	31	resulting	result	VBG
cord-014597-66vd2mdu	284	32	data	datum	NNS
cord-014597-66vd2mdu	284	33	collection	collection	NN
cord-014597-66vd2mdu	284	34	and	and	CC
cord-014597-66vd2mdu	284	35	aggregation	aggregation	NN
cord-014597-66vd2mdu	284	36	can	can	MD
cord-014597-66vd2mdu	284	37	be	be	VB
cord-014597-66vd2mdu	284	38	streamlined	streamline	VBN
cord-014597-66vd2mdu	284	39	.	.	.
cord-014597-66vd2mdu	285	1	Genedata	Genedata	NNP
cord-014597-66vd2mdu	285	2	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	285	3	helps	help	VBZ
cord-014597-66vd2mdu	285	4	to	to	TO
cord-014597-66vd2mdu	285	5	establish	establish	VB
cord-014597-66vd2mdu	285	6	more	more	RBR
cord-014597-66vd2mdu	285	7	productive	productive	JJ
cord-014597-66vd2mdu	285	8	processes	process	NNS
cord-014597-66vd2mdu	285	9	by	by	IN
cord-014597-66vd2mdu	285	10	offering	offer	VBG
cord-014597-66vd2mdu	285	11	support	support	NN
cord-014597-66vd2mdu	285	12	and	and	CC
cord-014597-66vd2mdu	285	13	integration	integration	NN
cord-014597-66vd2mdu	285	14	for	for	IN
cord-014597-66vd2mdu	285	15	automation	automation	NN
cord-014597-66vd2mdu	285	16	stations	station	NNS
cord-014597-66vd2mdu	285	17	and	and	CC
cord-014597-66vd2mdu	285	18	measurement	measurement	NN
cord-014597-66vd2mdu	285	19	devices	device	NNS
cord-014597-66vd2mdu	285	20	.	.	.
cord-014597-66vd2mdu	286	1	Thanks	thank	NNS
cord-014597-66vd2mdu	286	2	to	to	IN
cord-014597-66vd2mdu	286	3	the	the	DT
cord-014597-66vd2mdu	286	4	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	286	5	workflow	workflow	NN
cord-014597-66vd2mdu	286	6	support	support	NN
cord-014597-66vd2mdu	286	7	and	and	CC
cord-014597-66vd2mdu	286	8	the	the	DT
cord-014597-66vd2mdu	286	9	possibility	possibility	NN
cord-014597-66vd2mdu	286	10	to	to	TO
cord-014597-66vd2mdu	286	11	integrate	integrate	VB
cord-014597-66vd2mdu	286	12	results	result	NNS
cord-014597-66vd2mdu	286	13	from	from	IN
cord-014597-66vd2mdu	286	14	cell	cell	NN
cord-014597-66vd2mdu	286	15	line	line	NN
cord-014597-66vd2mdu	286	16	stability	stability	NN
cord-014597-66vd2mdu	286	17	experiments	experiment	NNS
cord-014597-66vd2mdu	286	18	,	,	,
cord-014597-66vd2mdu	286	19	product	product	NN
cord-014597-66vd2mdu	286	20	quality	quality	NN
cord-014597-66vd2mdu	286	21	assessment	assessment	NN
cord-014597-66vd2mdu	286	22	,	,	,
cord-014597-66vd2mdu	286	23	and	and	CC
cord-014597-66vd2mdu	286	24	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	286	25	suitability	suitability	NN
cord-014597-66vd2mdu	286	26	tests	test	NNS
cord-014597-66vd2mdu	286	27	,	,	,
cord-014597-66vd2mdu	286	28	Genedata	Genedata	NNP
cord-014597-66vd2mdu	286	29	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	286	30	provides	provide	VBZ
cord-014597-66vd2mdu	286	31	a	a	DT
cord-014597-66vd2mdu	286	32	unique	unique	JJ
cord-014597-66vd2mdu	286	33	way	way	NN
cord-014597-66vd2mdu	286	34	to	to	TO
cord-014597-66vd2mdu	286	35	evaluate	evaluate	VB
cord-014597-66vd2mdu	286	36	cell	cell	NN
cord-014597-66vd2mdu	286	37	lines	line	NNS
cord-014597-66vd2mdu	286	38	.	.	.
cord-014597-66vd2mdu	287	1	Comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	287	2	analysis	analysis	NN
cord-014597-66vd2mdu	287	3	of	of	IN
cord-014597-66vd2mdu	287	4	all	all	DT
cord-014597-66vd2mdu	287	5	data	datum	NNS
cord-014597-66vd2mdu	287	6	collected	collect	VBN
cord-014597-66vd2mdu	287	7	in	in	IN
cord-014597-66vd2mdu	287	8	the	the	DT
cord-014597-66vd2mdu	287	9	process	process	NN
cord-014597-66vd2mdu	287	10	helps	help	VBZ
cord-014597-66vd2mdu	287	11	to	to	TO
cord-014597-66vd2mdu	287	12	ensure	ensure	VB
cord-014597-66vd2mdu	287	13	the	the	DT
cord-014597-66vd2mdu	287	14	highest	high	JJS
cord-014597-66vd2mdu	287	15	possible	possible	JJ
cord-014597-66vd2mdu	287	16	quality	quality	NN
cord-014597-66vd2mdu	287	17	and	and	CC
cord-014597-66vd2mdu	287	18	minimize	minimize	VB
cord-014597-66vd2mdu	287	19	the	the	DT
cord-014597-66vd2mdu	287	20	time	time	NN
cord-014597-66vd2mdu	287	21	and	and	CC
cord-014597-66vd2mdu	287	22	resources	resource	NNS
cord-014597-66vd2mdu	287	23	needed	need	VBN
cord-014597-66vd2mdu	287	24	for	for	IN
cord-014597-66vd2mdu	287	25	data	datum	NNS
cord-014597-66vd2mdu	287	26	analysis	analysis	NN
cord-014597-66vd2mdu	287	27	and	and	CC
cord-014597-66vd2mdu	287	28	management	management	NN
cord-014597-66vd2mdu	287	29	.	.	.
cord-014597-66vd2mdu	288	1	Integration	integration	NN
cord-014597-66vd2mdu	288	2	of	of	IN
cord-014597-66vd2mdu	288	3	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	288	4	data	datum	NNS
cord-014597-66vd2mdu	288	5	analysis	analysis	NN
cord-014597-66vd2mdu	288	6	and	and	CC
cord-014597-66vd2mdu	288	7	visualization	visualization	NN
cord-014597-66vd2mdu	288	8	with	with	IN
cord-014597-66vd2mdu	288	9	other	other	JJ
cord-014597-66vd2mdu	288	10	parameters	parameter	NNS
cord-014597-66vd2mdu	288	11	measured	measure	VBN
cord-014597-66vd2mdu	288	12	in	in	IN
cord-014597-66vd2mdu	288	13	cell	cell	NN
cord-014597-66vd2mdu	288	14	line	line	NN
cord-014597-66vd2mdu	288	15	development	development	NN
cord-014597-66vd2mdu	288	16	,	,	,
cord-014597-66vd2mdu	288	17	streamlines	streamline	VBZ
cord-014597-66vd2mdu	288	18	clone	clone	NN
cord-014597-66vd2mdu	288	19	evaluation	evaluation	NN
cord-014597-66vd2mdu	288	20	in	in	IN
cord-014597-66vd2mdu	288	21	micro	micro	NNS
cord-014597-66vd2mdu	288	22	-	-	NNS
cord-014597-66vd2mdu	288	23	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	288	24	and	and	CC
cord-014597-66vd2mdu	288	25	supports	support	VBZ
cord-014597-66vd2mdu	288	26	highthroughput	highthroughput	NN
cord-014597-66vd2mdu	288	27	operations	operation	NNS
cord-014597-66vd2mdu	288	28	.	.	.
cord-014597-66vd2mdu	289	1	Genedata	Genedata	NNP
cord-014597-66vd2mdu	289	2	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	289	3	comprehensively	comprehensively	RB
cord-014597-66vd2mdu	289	4	tracks	track	VBZ
cord-014597-66vd2mdu	289	5	the	the	DT
cord-014597-66vd2mdu	289	6	full	full	JJ
cord-014597-66vd2mdu	289	7	clone	clone	NN
cord-014597-66vd2mdu	289	8	history	history	NN
cord-014597-66vd2mdu	289	9	from	from	IN
cord-014597-66vd2mdu	289	10	the	the	DT
cord-014597-66vd2mdu	289	11	origin	origin	NN
cord-014597-66vd2mdu	289	12	of	of	IN
cord-014597-66vd2mdu	289	13	the	the	DT
cord-014597-66vd2mdu	289	14	host	host	NN
cord-014597-66vd2mdu	289	15	cell	cell	NN
cord-014597-66vd2mdu	289	16	line	line	NN
cord-014597-66vd2mdu	289	17	to	to	IN
cord-014597-66vd2mdu	289	18	the	the	DT
cord-014597-66vd2mdu	289	19	generation	generation	NN
cord-014597-66vd2mdu	289	20	of	of	IN
cord-014597-66vd2mdu	289	21	the	the	DT
cord-014597-66vd2mdu	289	22	validated	validate	VBN
cord-014597-66vd2mdu	289	23	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	289	24	producer	producer	NN
cord-014597-66vd2mdu	289	25	cell	cell	NN
cord-014597-66vd2mdu	289	26	line	line	NN
cord-014597-66vd2mdu	289	27	.	.	.
cord-014597-66vd2mdu	290	1	For	for	IN
cord-014597-66vd2mdu	290	2	promising	promising	JJ
cord-014597-66vd2mdu	290	3	clones	clone	NNS
cord-014597-66vd2mdu	290	4	,	,	,
cord-014597-66vd2mdu	290	5	the	the	DT
cord-014597-66vd2mdu	290	6	clone	clone	NN
cord-014597-66vd2mdu	290	7	history	history	NN
cord-014597-66vd2mdu	290	8	report	report	NN
cord-014597-66vd2mdu	290	9	can	can	MD
cord-014597-66vd2mdu	290	10	be	be	VB
cord-014597-66vd2mdu	290	11	generated	generate	VBN
cord-014597-66vd2mdu	290	12	with	with	IN
cord-014597-66vd2mdu	290	13	one	one	CD
cord-014597-66vd2mdu	290	14	click	click	NN
cord-014597-66vd2mdu	290	15	.	.	.
cord-014597-66vd2mdu	291	1	Besides	besides	IN
cord-014597-66vd2mdu	291	2	supporting	support	VBG
cord-014597-66vd2mdu	291	3	cell	cell	NN
cord-014597-66vd2mdu	291	4	line	line	NN
cord-014597-66vd2mdu	291	5	development	development	NN
cord-014597-66vd2mdu	291	6	,	,	,
cord-014597-66vd2mdu	291	7	Genedata	Genedata	NNP
cord-014597-66vd2mdu	291	8	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	291	9	is	be	VBZ
cord-014597-66vd2mdu	291	10	a	a	DT
cord-014597-66vd2mdu	291	11	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	291	12	platform	platform	NN
cord-014597-66vd2mdu	291	13	capable	capable	JJ
cord-014597-66vd2mdu	291	14	of	of	IN
cord-014597-66vd2mdu	291	15	tracking	track	VBG
cord-014597-66vd2mdu	291	16	the	the	DT
cord-014597-66vd2mdu	291	17	complete	complete	JJ
cord-014597-66vd2mdu	291	18	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	291	19	development	development	NN
cord-014597-66vd2mdu	291	20	process	process	NN
cord-014597-66vd2mdu	291	21	.	.	.
cord-014597-66vd2mdu	292	1	In	in	IN
cord-014597-66vd2mdu	292	2	2012	2012	CD
cord-014597-66vd2mdu	292	3	,	,	,
cord-014597-66vd2mdu	292	4	14.1	14.1	CD
cord-014597-66vd2mdu	292	5	million	million	CD
cord-014597-66vd2mdu	292	6	people	people	NNS
cord-014597-66vd2mdu	292	7	suffered	suffer	VBD
cord-014597-66vd2mdu	292	8	from	from	IN
cord-014597-66vd2mdu	292	9	cancer	cancer	NN
cord-014597-66vd2mdu	292	10	[	[	-LRB-
cord-014597-66vd2mdu	292	11	1	1	CD
cord-014597-66vd2mdu	292	12	]	]	-RRB-
cord-014597-66vd2mdu	292	13	making	make	VBG
cord-014597-66vd2mdu	292	14	it	-PRON-	PRP
cord-014597-66vd2mdu	292	15	to	to	IN
cord-014597-66vd2mdu	292	16	a	a	DT
cord-014597-66vd2mdu	292	17	major	major	JJ
cord-014597-66vd2mdu	292	18	concern	concern	NN
cord-014597-66vd2mdu	292	19	of	of	IN
cord-014597-66vd2mdu	292	20	our	-PRON-	PRP$
cord-014597-66vd2mdu	292	21	society	society	NN
cord-014597-66vd2mdu	292	22	.	.	.
cord-014597-66vd2mdu	293	1	Since	since	IN
cord-014597-66vd2mdu	293	2	common	common	JJ
cord-014597-66vd2mdu	293	3	cancer	cancer	NN
cord-014597-66vd2mdu	293	4	treatment	treatment	NN
cord-014597-66vd2mdu	293	5	is	be	VBZ
cord-014597-66vd2mdu	293	6	limited	limit	VBN
cord-014597-66vd2mdu	293	7	and	and	CC
cord-014597-66vd2mdu	293	8	not	not	RB
cord-014597-66vd2mdu	293	9	effective	effective	JJ
cord-014597-66vd2mdu	293	10	for	for	IN
cord-014597-66vd2mdu	293	11	late	late	JJ
cord-014597-66vd2mdu	293	12	stage	stage	NN
cord-014597-66vd2mdu	293	13	carcinoma	carcinoma	NN
cord-014597-66vd2mdu	293	14	,	,	,
cord-014597-66vd2mdu	293	15	alternative	alternative	JJ
cord-014597-66vd2mdu	293	16	methods	method	NNS
cord-014597-66vd2mdu	293	17	are	be	VBP
cord-014597-66vd2mdu	293	18	needed	need	VBN
cord-014597-66vd2mdu	293	19	to	to	TO
cord-014597-66vd2mdu	293	20	reduce	reduce	VB
cord-014597-66vd2mdu	293	21	the	the	DT
cord-014597-66vd2mdu	293	22	high	high	JJ
cord-014597-66vd2mdu	293	23	mortality	mortality	NN
cord-014597-66vd2mdu	293	24	rate	rate	NN
cord-014597-66vd2mdu	293	25	of	of	IN
cord-014597-66vd2mdu	293	26	cancer	cancer	NN
cord-014597-66vd2mdu	293	27	patients	patient	NNS
cord-014597-66vd2mdu	293	28	.	.	.
cord-014597-66vd2mdu	294	1	One	one	CD
cord-014597-66vd2mdu	294	2	alternative	alternative	JJ
cord-014597-66vd2mdu	294	3	approach	approach	NN
cord-014597-66vd2mdu	294	4	is	be	VBZ
cord-014597-66vd2mdu	294	5	the	the	DT
cord-014597-66vd2mdu	294	6	application	application	NN
cord-014597-66vd2mdu	294	7	of	of	IN
cord-014597-66vd2mdu	294	8	the	the	DT
cord-014597-66vd2mdu	294	9	oncolytic	oncolytic	JJ
cord-014597-66vd2mdu	294	10	Measles	Measles	NNP
cord-014597-66vd2mdu	294	11	virus	virus	NN
cord-014597-66vd2mdu	294	12	(	(	-LRB-
cord-014597-66vd2mdu	294	13	OMV	OMV	NNP
cord-014597-66vd2mdu	294	14	)	)	-RRB-
cord-014597-66vd2mdu	294	15	,	,	,
cord-014597-66vd2mdu	294	16	because	because	IN
cord-014597-66vd2mdu	294	17	OMV	OMV	NNP
cord-014597-66vd2mdu	294	18	has	have	VBZ
cord-014597-66vd2mdu	294	19	a	a	DT
cord-014597-66vd2mdu	294	20	natural	natural	JJ
cord-014597-66vd2mdu	294	21	affinity	affinity	NN
cord-014597-66vd2mdu	294	22	against	against	IN
cord-014597-66vd2mdu	294	23	cancer	cancer	NN
cord-014597-66vd2mdu	294	24	cells	cell	NNS
cord-014597-66vd2mdu	294	25	.	.	.
cord-014597-66vd2mdu	295	1	The	the	DT
cord-014597-66vd2mdu	295	2	major	major	JJ
cord-014597-66vd2mdu	295	3	drawbacks	drawback	NNS
cord-014597-66vd2mdu	295	4	of	of	IN
cord-014597-66vd2mdu	295	5	OMV	OMV	NNP
cord-014597-66vd2mdu	295	6	is	be	VBZ
cord-014597-66vd2mdu	295	7	to	to	TO
cord-014597-66vd2mdu	295	8	produce	produce	VB
cord-014597-66vd2mdu	295	9	the	the	DT
cord-014597-66vd2mdu	295	10	extremely	extremely	RB
cord-014597-66vd2mdu	295	11	high	high	JJ
cord-014597-66vd2mdu	295	12	amount	amount	NN
cord-014597-66vd2mdu	295	13	of	of	IN
cord-014597-66vd2mdu	295	14	at	at	RB
cord-014597-66vd2mdu	295	15	least	least	RBS
cord-014597-66vd2mdu	295	16	10	10	CD
cord-014597-66vd2mdu	295	17	11	11	CD
cord-014597-66vd2mdu	295	18	TCID	TCID	NNP
cord-014597-66vd2mdu	295	19	50	50	CD
cord-014597-66vd2mdu	295	20	(	(	-LRB-
cord-014597-66vd2mdu	295	21	50	50	CD
cord-014597-66vd2mdu	295	22	%	%	NN
cord-014597-66vd2mdu	295	23	tissue	tissue	NN
cord-014597-66vd2mdu	295	24	culture	culture	NN
cord-014597-66vd2mdu	295	25	infective	infective	JJ
cord-014597-66vd2mdu	295	26	dose	dose	NN
cord-014597-66vd2mdu	295	27	)	)	-RRB-
cord-014597-66vd2mdu	295	28	per	per	IN
cord-014597-66vd2mdu	295	29	dose	dose	NN
cord-014597-66vd2mdu	295	30	[	[	-LRB-
cord-014597-66vd2mdu	295	31	2	2	CD
cord-014597-66vd2mdu	295	32	]	]	-RRB-
cord-014597-66vd2mdu	295	33	which	which	WDT
cord-014597-66vd2mdu	295	34	is	be	VBZ
cord-014597-66vd2mdu	295	35	needed	need	VBN
cord-014597-66vd2mdu	295	36	.	.	.
cord-014597-66vd2mdu	296	1	To	to	TO
cord-014597-66vd2mdu	296	2	solve	solve	VB
cord-014597-66vd2mdu	296	3	this	this	DT
cord-014597-66vd2mdu	296	4	problem	problem	NN
cord-014597-66vd2mdu	296	5	,	,	,
cord-014597-66vd2mdu	296	6	a	a	DT
cord-014597-66vd2mdu	296	7	high	high	JJ
cord-014597-66vd2mdu	296	8	titer	titer	NN
cord-014597-66vd2mdu	296	9	process	process	NN
cord-014597-66vd2mdu	296	10	must	must	MD
cord-014597-66vd2mdu	296	11	be	be	VB
cord-014597-66vd2mdu	296	12	established	establish	VBN
cord-014597-66vd2mdu	296	13	including	include	VBG
cord-014597-66vd2mdu	296	14	an	an	DT
cord-014597-66vd2mdu	296	15	efficient	efficient	JJ
cord-014597-66vd2mdu	296	16	downstream	downstream	JJ
cord-014597-66vd2mdu	296	17	processing	processing	NN
cord-014597-66vd2mdu	296	18	(	(	-LRB-
cord-014597-66vd2mdu	296	19	DSP	DSP	NNP
cord-014597-66vd2mdu	296	20	)	)	-RRB-
cord-014597-66vd2mdu	296	21	.	.	.
cord-014597-66vd2mdu	297	1	We	-PRON-	PRP
cord-014597-66vd2mdu	297	2	developed	develop	VBD
cord-014597-66vd2mdu	297	3	an	an	DT
cord-014597-66vd2mdu	297	4	appropriate	appropriate	JJ
cord-014597-66vd2mdu	297	5	upstream	upstream	JJ
cord-014597-66vd2mdu	297	6	processing	processing	NN
cord-014597-66vd2mdu	297	7	and	and	CC
cord-014597-66vd2mdu	297	8	are	be	VBP
cord-014597-66vd2mdu	297	9	able	able	JJ
cord-014597-66vd2mdu	297	10	to	to	TO
cord-014597-66vd2mdu	297	11	produce	produce	VB
cord-014597-66vd2mdu	297	12	10	10	CD
cord-014597-66vd2mdu	297	13	10	10	CD
cord-014597-66vd2mdu	297	14	-10	-10	CD
cord-014597-66vd2mdu	297	15	11	11	CD
cord-014597-66vd2mdu	298	1	TCID	TCID	NNP
cord-014597-66vd2mdu	298	2	50	50	CD
cord-014597-66vd2mdu	298	3	mL	ml	NN
cord-014597-66vd2mdu	299	1	-1	-1	.
cord-014597-66vd2mdu	300	1	in	in	IN
cord-014597-66vd2mdu	300	2	a	a	DT
cord-014597-66vd2mdu	300	3	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	300	4	with	with	IN
cord-014597-66vd2mdu	300	5	0.5	0.5	CD
cord-014597-66vd2mdu	300	6	L	l	NN
cord-014597-66vd2mdu	300	7	working	working	NN
cord-014597-66vd2mdu	300	8	volume	volume	NN
cord-014597-66vd2mdu	300	9	[	[	-LRB-
cord-014597-66vd2mdu	300	10	3	3	CD
cord-014597-66vd2mdu	300	11	]	]	-RRB-
cord-014597-66vd2mdu	300	12	.	.	.
cord-014597-66vd2mdu	301	1	Now	now	RB
cord-014597-66vd2mdu	301	2	,	,	,
cord-014597-66vd2mdu	301	3	we	-PRON-	PRP
cord-014597-66vd2mdu	301	4	focus	focus	VBP
cord-014597-66vd2mdu	301	5	on	on	IN
cord-014597-66vd2mdu	301	6	the	the	DT
cord-014597-66vd2mdu	301	7	DSP	DSP	NNP
cord-014597-66vd2mdu	301	8	part	part	NN
cord-014597-66vd2mdu	301	9	.	.	.
cord-014597-66vd2mdu	302	1	The	the	DT
cord-014597-66vd2mdu	302	2	following	following	JJ
cord-014597-66vd2mdu	302	3	study	study	NN
cord-014597-66vd2mdu	302	4	tested	test	VBD
cord-014597-66vd2mdu	302	5	the	the	DT
cord-014597-66vd2mdu	302	6	application	application	NN
cord-014597-66vd2mdu	302	7	of	of	IN
cord-014597-66vd2mdu	302	8	charged	charge	VBN
cord-014597-66vd2mdu	302	9	depth	depth	NN
cord-014597-66vd2mdu	302	10	filters	filter	NNS
cord-014597-66vd2mdu	302	11	for	for	IN
cord-014597-66vd2mdu	302	12	the	the	DT
cord-014597-66vd2mdu	302	13	OMV	OMV	NNP
cord-014597-66vd2mdu	302	14	clarification	clarification	NN
cord-014597-66vd2mdu	302	15	.	.	.
cord-014597-66vd2mdu	303	1	In	in	IN
cord-014597-66vd2mdu	303	2	contrast	contrast	NN
cord-014597-66vd2mdu	303	3	to	to	IN
cord-014597-66vd2mdu	303	4	common	common	JJ
cord-014597-66vd2mdu	303	5	DSP	DSP	NNP
cord-014597-66vd2mdu	303	6	schemes	scheme	NNS
cord-014597-66vd2mdu	303	7	,	,	,
cord-014597-66vd2mdu	303	8	a	a	DT
cord-014597-66vd2mdu	303	9	depletion	depletion	NN
cord-014597-66vd2mdu	303	10	of	of	IN
cord-014597-66vd2mdu	303	11	virus	virus	NN
cord-014597-66vd2mdu	303	12	particles	particle	NNS
cord-014597-66vd2mdu	303	13	or	or	CC
cord-014597-66vd2mdu	303	14	a	a	DT
cord-014597-66vd2mdu	303	15	loss	loss	NN
cord-014597-66vd2mdu	303	16	of	of	IN
cord-014597-66vd2mdu	303	17	infectivity	infectivity	NN
cord-014597-66vd2mdu	303	18	is	be	VBZ
cord-014597-66vd2mdu	303	19	not	not	RB
cord-014597-66vd2mdu	303	20	desired	desire	VBN
cord-014597-66vd2mdu	303	21	.	.	.
cord-014597-66vd2mdu	304	1	The	the	DT
cord-014597-66vd2mdu	304	2	aim	aim	NN
cord-014597-66vd2mdu	304	3	is	be	VBZ
cord-014597-66vd2mdu	304	4	a	a	DT
cord-014597-66vd2mdu	304	5	reduction	reduction	NN
cord-014597-66vd2mdu	304	6	of	of	IN
cord-014597-66vd2mdu	304	7	protein	protein	NN
cord-014597-66vd2mdu	304	8	content	content	NN
cord-014597-66vd2mdu	304	9	and	and	CC
cord-014597-66vd2mdu	304	10	DNA	dna	NN
cord-014597-66vd2mdu	304	11	with	with	IN
cord-014597-66vd2mdu	304	12	minimal	minimal	JJ
cord-014597-66vd2mdu	304	13	loss	loss	NN
cord-014597-66vd2mdu	304	14	of	of	IN
cord-014597-66vd2mdu	304	15	infective	infective	JJ
cord-014597-66vd2mdu	304	16	OMV	omv	NN
cord-014597-66vd2mdu	304	17	.	.	.
cord-014597-66vd2mdu	305	1	Further	further	RB
cord-014597-66vd2mdu	305	2	,	,	,
cord-014597-66vd2mdu	305	3	we	-PRON-	PRP
cord-014597-66vd2mdu	305	4	investigated	investigate	VBD
cord-014597-66vd2mdu	305	5	the	the	DT
cord-014597-66vd2mdu	305	6	influence	influence	NN
cord-014597-66vd2mdu	305	7	of	of	IN
cord-014597-66vd2mdu	305	8	the	the	DT
cord-014597-66vd2mdu	305	9	cell	cell	NN
cord-014597-66vd2mdu	305	10	culture	culture	NN
cord-014597-66vd2mdu	305	11	medium	medium	NN
cord-014597-66vd2mdu	305	12	on	on	IN
cord-014597-66vd2mdu	305	13	the	the	DT
cord-014597-66vd2mdu	305	14	depth	depth	NN
cord-014597-66vd2mdu	305	15	filtration	filtration	NN
cord-014597-66vd2mdu	305	16	process	process	NN
cord-014597-66vd2mdu	305	17	.	.	.
cord-014597-66vd2mdu	306	1	To	to	TO
cord-014597-66vd2mdu	306	2	explore	explore	VB
cord-014597-66vd2mdu	306	3	the	the	DT
cord-014597-66vd2mdu	306	4	influence	influence	NN
cord-014597-66vd2mdu	306	5	of	of	IN
cord-014597-66vd2mdu	306	6	the	the	DT
cord-014597-66vd2mdu	306	7	surrounding	surround	VBG
cord-014597-66vd2mdu	306	8	cell	cell	NN
cord-014597-66vd2mdu	306	9	culture	culture	NN
cord-014597-66vd2mdu	306	10	medium	medium	NN
cord-014597-66vd2mdu	306	11	on	on	IN
cord-014597-66vd2mdu	306	12	the	the	DT
cord-014597-66vd2mdu	306	13	depth	depth	NN
cord-014597-66vd2mdu	306	14	filtration	filtration	NN
cord-014597-66vd2mdu	306	15	performance	performance	NN
cord-014597-66vd2mdu	306	16	,	,	,
cord-014597-66vd2mdu	306	17	OMV	OMV	NNP
cord-014597-66vd2mdu	306	18	was	be	VBD
cord-014597-66vd2mdu	306	19	either	either	CC
cord-014597-66vd2mdu	306	20	produced	produce	VBN
cord-014597-66vd2mdu	306	21	in	in	IN
cord-014597-66vd2mdu	306	22	serum	serum	NN
cord-014597-66vd2mdu	306	23	-	-	HYPH
cord-014597-66vd2mdu	306	24	free	free	JJ
cord-014597-66vd2mdu	306	25	medium	medium	NN
cord-014597-66vd2mdu	306	26	(	(	-LRB-
cord-014597-66vd2mdu	306	27	VP	VP	NNP
cord-014597-66vd2mdu	306	28	-	-	HYPH
cord-014597-66vd2mdu	306	29	SFM	SFM	NNP
cord-014597-66vd2mdu	306	30	)	)	-RRB-
cord-014597-66vd2mdu	306	31	or	or	CC
cord-014597-66vd2mdu	306	32	serum	serum	NN
cord-014597-66vd2mdu	306	33	-	-	HYPH
cord-014597-66vd2mdu	306	34	containing	contain	VBG
cord-014597-66vd2mdu	306	35	medium	medium	NN
cord-014597-66vd2mdu	306	36	(	(	-LRB-
cord-014597-66vd2mdu	306	37	DMEM	DMEM	NNP
cord-014597-66vd2mdu	306	38	+	+	CC
cord-014597-66vd2mdu	306	39	10	10	CD
cord-014597-66vd2mdu	306	40	%	%	NN
cord-014597-66vd2mdu	306	41	FCS	FCS	NNP
cord-014597-66vd2mdu	306	42	)	)	-RRB-
cord-014597-66vd2mdu	306	43	.	.	.
cord-014597-66vd2mdu	307	1	The	the	DT
cord-014597-66vd2mdu	307	2	production	production	NN
cord-014597-66vd2mdu	307	3	was	be	VBD
cord-014597-66vd2mdu	307	4	done	do	VBN
cord-014597-66vd2mdu	307	5	in	in	IN
cord-014597-66vd2mdu	307	6	a	a	DT
cord-014597-66vd2mdu	307	7	STR	str	NN
cord-014597-66vd2mdu	307	8	with	with	IN
cord-014597-66vd2mdu	307	9	a	a	DT
cord-014597-66vd2mdu	307	10	working	working	JJ
cord-014597-66vd2mdu	307	11	volume	volume	NN
cord-014597-66vd2mdu	307	12	of	of	IN
cord-014597-66vd2mdu	307	13	0.5	0.5	CD
cord-014597-66vd2mdu	307	14	L	L	NNP
cord-014597-66vd2mdu	307	15	as	as	IN
cord-014597-66vd2mdu	307	16	described	describe	VBN
cord-014597-66vd2mdu	307	17	in	in	IN
cord-014597-66vd2mdu	307	18	[	[	-LRB-
cord-014597-66vd2mdu	307	19	3	3	CD
cord-014597-66vd2mdu	307	20	]	]	-RRB-
cord-014597-66vd2mdu	307	21	.	.	.
cord-014597-66vd2mdu	308	1	Cells	cell	NNS
cord-014597-66vd2mdu	308	2	and	and	CC
cord-014597-66vd2mdu	308	3	carriers	carrier	NNS
cord-014597-66vd2mdu	308	4	were	be	VBD
cord-014597-66vd2mdu	308	5	separated	separate	VBN
cord-014597-66vd2mdu	308	6	with	with	IN
cord-014597-66vd2mdu	308	7	an	an	DT
cord-014597-66vd2mdu	308	8	Opticap	Opticap	NNP
cord-014597-66vd2mdu	308	9	XL	XL	NNP
cord-014597-66vd2mdu	308	10	1-module	1-module	CD
cord-014597-66vd2mdu	309	1	(	(	-LRB-
cord-014597-66vd2mdu	309	2	Polygard	Polygard	NNP
cord-014597-66vd2mdu	309	3	-	-	HYPH
cord-014597-66vd2mdu	309	4	CR	CR	NNP
cord-014597-66vd2mdu	309	5	;	;	:
cord-014597-66vd2mdu	309	6	5	5	CD
cord-014597-66vd2mdu	309	7	μm	μm	NN
cord-014597-66vd2mdu	309	8	;	;	:
cord-014597-66vd2mdu	309	9	Merck	Merck	NNP
cord-014597-66vd2mdu	309	10	)	)	-RRB-
cord-014597-66vd2mdu	309	11	.	.	.
cord-014597-66vd2mdu	310	1	For	for	IN
cord-014597-66vd2mdu	310	2	the	the	DT
cord-014597-66vd2mdu	310	3	depth	depth	NN
cord-014597-66vd2mdu	310	4	filtration	filtration	NN
cord-014597-66vd2mdu	310	5	Millistak+	millistak+	NN
cord-014597-66vd2mdu	310	6	CE50	CE50	NNP
cord-014597-66vd2mdu	310	7	filters	filter	NNS
cord-014597-66vd2mdu	310	8	(	(	-LRB-
cord-014597-66vd2mdu	310	9	Merck	Merck	NNP
cord-014597-66vd2mdu	310	10	)	)	-RRB-
cord-014597-66vd2mdu	310	11	were	be	VBD
cord-014597-66vd2mdu	310	12	used	use	VBN
cord-014597-66vd2mdu	310	13	.	.	.
cord-014597-66vd2mdu	311	1	The	the	DT
cord-014597-66vd2mdu	311	2	filter	filter	NN
cord-014597-66vd2mdu	311	3	material	material	NN
cord-014597-66vd2mdu	311	4	was	be	VBD
cord-014597-66vd2mdu	311	5	autoclaved	autoclave	VBN
cord-014597-66vd2mdu	311	6	and	and	CC
cord-014597-66vd2mdu	311	7	rinsed	rinse	VBN
cord-014597-66vd2mdu	311	8	with	with	IN
cord-014597-66vd2mdu	311	9	25	25	CD
cord-014597-66vd2mdu	311	10	mL	mL	NNS
cord-014597-66vd2mdu	311	11	of	of	IN
cord-014597-66vd2mdu	311	12	20	20	CD
cord-014597-66vd2mdu	311	13	mM	mM	NNP
cord-014597-66vd2mdu	311	14	Tris	Tris	NNP
cord-014597-66vd2mdu	311	15	-	-	HYPH
cord-014597-66vd2mdu	311	16	HCl	HCl	NNP
cord-014597-66vd2mdu	311	17	(	(	-LRB-
cord-014597-66vd2mdu	311	18	pH=7.4	pH=7.4	NNP
cord-014597-66vd2mdu	311	19	)	)	-RRB-
cord-014597-66vd2mdu	311	20	.	.	.
cord-014597-66vd2mdu	312	1	The	the	DT
cord-014597-66vd2mdu	312	2	virus	virus	NN
cord-014597-66vd2mdu	312	3	suspension	suspension	NN
cord-014597-66vd2mdu	312	4	was	be	VBD
cord-014597-66vd2mdu	312	5	filtered	filter	VBN
cord-014597-66vd2mdu	312	6	with	with	IN
cord-014597-66vd2mdu	312	7	a	a	DT
cord-014597-66vd2mdu	312	8	load	load	NN
cord-014597-66vd2mdu	312	9	of	of	IN
cord-014597-66vd2mdu	312	10	50	50	CD
cord-014597-66vd2mdu	312	11	L	l	NN
cord-014597-66vd2mdu	312	12	m	m	NN
cord-014597-66vd2mdu	312	13	-2	-2	NNP
cord-014597-66vd2mdu	312	14	using	use	VBG
cord-014597-66vd2mdu	312	15	a	a	DT
cord-014597-66vd2mdu	312	16	peristaltic	peristaltic	JJ
cord-014597-66vd2mdu	312	17	pump	pump	NN
cord-014597-66vd2mdu	312	18	(	(	-LRB-
cord-014597-66vd2mdu	312	19	ISM931C	ISM931C	NNP
cord-014597-66vd2mdu	312	20	;	;	:
cord-014597-66vd2mdu	312	21	Ismatec	Ismatec	NNP
cord-014597-66vd2mdu	312	22	)	)	-RRB-
cord-014597-66vd2mdu	312	23	applying	apply	VBG
cord-014597-66vd2mdu	312	24	a	a	DT
cord-014597-66vd2mdu	312	25	flux	flux	NN
cord-014597-66vd2mdu	312	26	of	of	IN
cord-014597-66vd2mdu	312	27	150	150	CD
cord-014597-66vd2mdu	312	28	L	l	NN
cord-014597-66vd2mdu	312	29	m	m	NN
cord-014597-66vd2mdu	312	30	-2	-2	NN
cord-014597-66vd2mdu	312	31	h	h	NN
cord-014597-66vd2mdu	312	32	-1	-1	NN
cord-014597-66vd2mdu	313	1	(	(	-LRB-
cord-014597-66vd2mdu	313	2	Fig	Fig	NNP
cord-014597-66vd2mdu	313	3	.	.	NNP
cord-014597-66vd2mdu	313	4	1	1	CD
cord-014597-66vd2mdu	313	5	)	)	-RRB-
cord-014597-66vd2mdu	313	6	.	.	.
cord-014597-66vd2mdu	314	1	Samples	sample	NNS
cord-014597-66vd2mdu	314	2	were	be	VBD
cord-014597-66vd2mdu	314	3	collected	collect	VBN
cord-014597-66vd2mdu	314	4	at	at	IN
cord-014597-66vd2mdu	314	5	the	the	DT
cord-014597-66vd2mdu	314	6	beginning	beginning	NN
cord-014597-66vd2mdu	314	7	and	and	CC
cord-014597-66vd2mdu	314	8	end	end	NN
cord-014597-66vd2mdu	314	9	of	of	IN
cord-014597-66vd2mdu	314	10	a	a	DT
cord-014597-66vd2mdu	314	11	filtration	filtration	NN
cord-014597-66vd2mdu	314	12	run	run	NN
cord-014597-66vd2mdu	314	13	.	.	.
cord-014597-66vd2mdu	315	1	The	the	DT
cord-014597-66vd2mdu	315	2	OMV	OMV	NNP
cord-014597-66vd2mdu	315	3	titer	titer	NN
cord-014597-66vd2mdu	315	4	(	(	-LRB-
cord-014597-66vd2mdu	315	5	TCID	TCID	NNP
cord-014597-66vd2mdu	315	6	50	50	CD
cord-014597-66vd2mdu	315	7	mL	ml	NN
cord-014597-66vd2mdu	315	8	-1	-1	NN
cord-014597-66vd2mdu	315	9	)	)	-RRB-
cord-014597-66vd2mdu	315	10	of	of	IN
cord-014597-66vd2mdu	315	11	the	the	DT
cord-014597-66vd2mdu	315	12	samples	sample	NNS
cord-014597-66vd2mdu	315	13	was	be	VBD
cord-014597-66vd2mdu	315	14	determined	determine	VBN
cord-014597-66vd2mdu	315	15	according	accord	VBG
cord-014597-66vd2mdu	315	16	to	to	IN
cord-014597-66vd2mdu	315	17	Kärber	Kärber	NNP
cord-014597-66vd2mdu	315	18	and	and	CC
cord-014597-66vd2mdu	315	19	Reed	Reed	NNP
cord-014597-66vd2mdu	315	20	[	[	-LRB-
cord-014597-66vd2mdu	315	21	4	4	CD
cord-014597-66vd2mdu	315	22	,	,	,
cord-014597-66vd2mdu	315	23	5	5	CD
cord-014597-66vd2mdu	315	24	]	]	-RRB-
cord-014597-66vd2mdu	315	25	.	.	.
cord-014597-66vd2mdu	316	1	Protein	protein	NN
cord-014597-66vd2mdu	316	2	content	content	NN
cord-014597-66vd2mdu	316	3	was	be	VBD
cord-014597-66vd2mdu	316	4	measured	measure	VBN
cord-014597-66vd2mdu	316	5	with	with	IN
cord-014597-66vd2mdu	316	6	the	the	DT
cord-014597-66vd2mdu	316	7	Pierce	Pierce	NNP
cord-014597-66vd2mdu	316	8	BCA	BCA	NNP
cord-014597-66vd2mdu	316	9	protein	protein	NN
cord-014597-66vd2mdu	316	10	assay	assay	NN
cord-014597-66vd2mdu	316	11	kit	kit	NN
cord-014597-66vd2mdu	316	12	(	(	-LRB-
cord-014597-66vd2mdu	316	13	ThermoFisher	ThermoFisher	NNP
cord-014597-66vd2mdu	316	14	Scientific	Scientific	NNP
cord-014597-66vd2mdu	316	15	)	)	-RRB-
cord-014597-66vd2mdu	316	16	according	accord	VBG
cord-014597-66vd2mdu	316	17	to	to	IN
cord-014597-66vd2mdu	316	18	the	the	DT
cord-014597-66vd2mdu	316	19	manufacturer	manufacturer	NN
cord-014597-66vd2mdu	316	20	's	's	POS
cord-014597-66vd2mdu	316	21	instructions	instruction	NNS
cord-014597-66vd2mdu	316	22	.	.	.
cord-014597-66vd2mdu	317	1	DNA	DNA	NNP
cord-014597-66vd2mdu	317	2	was	be	VBD
cord-014597-66vd2mdu	317	3	measured	measure	VBN
cord-014597-66vd2mdu	317	4	by	by	IN
cord-014597-66vd2mdu	317	5	a	a	DT
cord-014597-66vd2mdu	317	6	microtiter	microtiter	NN
cord-014597-66vd2mdu	317	7	assay	assay	NN
cord-014597-66vd2mdu	317	8	using	use	VBG
cord-014597-66vd2mdu	317	9	Quant	Quant	NNP
cord-014597-66vd2mdu	317	10	-	-	:
cord-014597-66vd2mdu	317	11	iT	it	PRP
cord-014597-66vd2mdu	317	12	PicoGreen	PicoGreen	NNP
cord-014597-66vd2mdu	317	13	dsDNA	dsdna	RB
cord-014597-66vd2mdu	317	14	reagent	reagent	NN
cord-014597-66vd2mdu	317	15	(	(	-LRB-
cord-014597-66vd2mdu	317	16	ThermoFisher	ThermoFisher	NNP
cord-014597-66vd2mdu	317	17	Scientific	Scientific	NNP
cord-014597-66vd2mdu	317	18	)	)	-RRB-
cord-014597-66vd2mdu	317	19	according	accord	VBG
cord-014597-66vd2mdu	317	20	to	to	IN
cord-014597-66vd2mdu	317	21	the	the	DT
cord-014597-66vd2mdu	317	22	manufacturer	manufacturer	NN
cord-014597-66vd2mdu	317	23	's	's	POS
cord-014597-66vd2mdu	317	24	instructions	instruction	NNS
cord-014597-66vd2mdu	317	25	.	.	.
cord-014597-66vd2mdu	318	1	We	-PRON-	PRP
cord-014597-66vd2mdu	318	2	found	find	VBD
cord-014597-66vd2mdu	318	3	that	that	IN
cord-014597-66vd2mdu	318	4	positively	positively	RB
cord-014597-66vd2mdu	318	5	charged	charge	VBN
cord-014597-66vd2mdu	318	6	depth	depth	NN
cord-014597-66vd2mdu	318	7	filters	filter	NNS
cord-014597-66vd2mdu	318	8	were	be	VBD
cord-014597-66vd2mdu	318	9	suitable	suitable	JJ
cord-014597-66vd2mdu	318	10	to	to	TO
cord-014597-66vd2mdu	318	11	clarify	clarify	VB
cord-014597-66vd2mdu	318	12	OMV	omv	NN
cord-014597-66vd2mdu	318	13	suspensions	suspension	NNS
cord-014597-66vd2mdu	318	14	.	.	.
cord-014597-66vd2mdu	319	1	The	the	DT
cord-014597-66vd2mdu	319	2	cell	cell	NN
cord-014597-66vd2mdu	319	3	culture	culture	NN
cord-014597-66vd2mdu	319	4	medium	medium	NN
cord-014597-66vd2mdu	319	5	,	,	,
cord-014597-66vd2mdu	319	6	in	in	IN
cord-014597-66vd2mdu	319	7	which	which	WDT
cord-014597-66vd2mdu	319	8	the	the	DT
cord-014597-66vd2mdu	319	9	OMV	OMV	NNP
cord-014597-66vd2mdu	319	10	was	be	VBD
cord-014597-66vd2mdu	319	11	produced	produce	VBN
cord-014597-66vd2mdu	319	12	,	,	,
cord-014597-66vd2mdu	319	13	influenced	influence	VBD
cord-014597-66vd2mdu	319	14	the	the	DT
cord-014597-66vd2mdu	319	15	outcome	outcome	NN
cord-014597-66vd2mdu	319	16	of	of	IN
cord-014597-66vd2mdu	319	17	the	the	DT
cord-014597-66vd2mdu	319	18	depth	depth	NN
cord-014597-66vd2mdu	319	19	filtration	filtration	NN
cord-014597-66vd2mdu	319	20	.	.	.
cord-014597-66vd2mdu	320	1	A	a	DT
cord-014597-66vd2mdu	320	2	log	log	NN
cord-014597-66vd2mdu	320	3	reduction	reduction	NN
cord-014597-66vd2mdu	320	4	value	value	NN
cord-014597-66vd2mdu	320	5	(	(	-LRB-
cord-014597-66vd2mdu	320	6	LRV	lrv	NN
cord-014597-66vd2mdu	320	7	)	)	-RRB-
cord-014597-66vd2mdu	320	8	of	of	IN
cord-014597-66vd2mdu	320	9	0.87	0.87	CD
cord-014597-66vd2mdu	320	10	was	be	VBD
cord-014597-66vd2mdu	320	11	determined	determine	VBN
cord-014597-66vd2mdu	320	12	for	for	IN
cord-014597-66vd2mdu	320	13	OMV	omv	NN
cord-014597-66vd2mdu	320	14	present	present	JJ
cord-014597-66vd2mdu	320	15	in	in	IN
cord-014597-66vd2mdu	320	16	serum	serum	NN
cord-014597-66vd2mdu	320	17	-	-	HYPH
cord-014597-66vd2mdu	320	18	containing	contain	VBG
cord-014597-66vd2mdu	320	19	medium	medium	NN
cord-014597-66vd2mdu	320	20	(	(	-LRB-
cord-014597-66vd2mdu	320	21	SCM	SCM	NNP
cord-014597-66vd2mdu	320	22	)	)	-RRB-
cord-014597-66vd2mdu	320	23	,	,	,
cord-014597-66vd2mdu	320	24	whereas	whereas	IN
cord-014597-66vd2mdu	320	25	the	the	DT
cord-014597-66vd2mdu	320	26	titer	titer	NN
cord-014597-66vd2mdu	320	27	of	of	IN
cord-014597-66vd2mdu	320	28	OMV	OMV	NNP
cord-014597-66vd2mdu	320	29	in	in	IN
cord-014597-66vd2mdu	320	30	serumfree	serumfree	JJ
cord-014597-66vd2mdu	320	31	medium	medium	NN
cord-014597-66vd2mdu	320	32	(	(	-LRB-
cord-014597-66vd2mdu	320	33	SFM	SFM	NNP
cord-014597-66vd2mdu	320	34	)	)	-RRB-
cord-014597-66vd2mdu	320	35	was	be	VBD
cord-014597-66vd2mdu	320	36	reduced	reduce	VBN
cord-014597-66vd2mdu	320	37	1.63	1.63	CD
cord-014597-66vd2mdu	320	38	log	log	NN
cord-014597-66vd2mdu	320	39	levels	level	NNS
cord-014597-66vd2mdu	320	40	.	.	.
cord-014597-66vd2mdu	321	1	This	this	DT
cord-014597-66vd2mdu	321	2	indicates	indicate	VBZ
cord-014597-66vd2mdu	321	3	that	that	IN
cord-014597-66vd2mdu	321	4	without	without	IN
cord-014597-66vd2mdu	321	5	serum	serum	NN
cord-014597-66vd2mdu	321	6	in	in	IN
cord-014597-66vd2mdu	321	7	the	the	DT
cord-014597-66vd2mdu	321	8	surrounding	surround	VBG
cord-014597-66vd2mdu	321	9	liquid	liquid	NN
cord-014597-66vd2mdu	321	10	,	,	,
cord-014597-66vd2mdu	321	11	OMV	OMV	NNP
cord-014597-66vd2mdu	321	12	will	will	MD
cord-014597-66vd2mdu	321	13	adsorb	adsorb	VB
cord-014597-66vd2mdu	321	14	to	to	IN
cord-014597-66vd2mdu	321	15	the	the	DT
cord-014597-66vd2mdu	321	16	filter	filter	NN
cord-014597-66vd2mdu	321	17	material	material	NN
cord-014597-66vd2mdu	321	18	.	.	.
cord-014597-66vd2mdu	322	1	However	however	RB
cord-014597-66vd2mdu	322	2	,	,	,
cord-014597-66vd2mdu	322	3	we	-PRON-	PRP
cord-014597-66vd2mdu	322	4	must	must	MD
cord-014597-66vd2mdu	322	5	evaluate	evaluate	VB
cord-014597-66vd2mdu	322	6	if	if	IN
cord-014597-66vd2mdu	322	7	the	the	DT
cord-014597-66vd2mdu	322	8	missing	miss	VBG
cord-014597-66vd2mdu	322	9	serum	serum	NN
cord-014597-66vd2mdu	322	10	or	or	CC
cord-014597-66vd2mdu	322	11	other	other	JJ
cord-014597-66vd2mdu	322	12	components	component	NNS
cord-014597-66vd2mdu	322	13	present	present	JJ
cord-014597-66vd2mdu	322	14	in	in	IN
cord-014597-66vd2mdu	322	15	SFM	SFM	NNP
cord-014597-66vd2mdu	322	16	are	be	VBP
cord-014597-66vd2mdu	322	17	responsible	responsible	JJ
cord-014597-66vd2mdu	322	18	for	for	IN
cord-014597-66vd2mdu	322	19	this	this	DT
cord-014597-66vd2mdu	322	20	effect	effect	NN
cord-014597-66vd2mdu	322	21	.	.	.
cord-014597-66vd2mdu	323	1	Total	total	JJ
cord-014597-66vd2mdu	323	2	protein	protein	NN
cord-014597-66vd2mdu	323	3	was	be	VBD
cord-014597-66vd2mdu	323	4	not	not	RB
cord-014597-66vd2mdu	323	5	relevantly	relevantly	RB
cord-014597-66vd2mdu	323	6	reduced	reduce	VBN
cord-014597-66vd2mdu	323	7	by	by	IN
cord-014597-66vd2mdu	323	8	the	the	DT
cord-014597-66vd2mdu	323	9	clarification	clarification	NN
cord-014597-66vd2mdu	323	10	using	use	VBG
cord-014597-66vd2mdu	323	11	charged	charge	VBN
cord-014597-66vd2mdu	323	12	depth	depth	NN
cord-014597-66vd2mdu	323	13	filters	filter	NNS
cord-014597-66vd2mdu	323	14	.	.	.
cord-014597-66vd2mdu	324	1	For	for	IN
cord-014597-66vd2mdu	324	2	OMV	OMV	NNP
cord-014597-66vd2mdu	324	3	present	present	JJ
cord-014597-66vd2mdu	324	4	in	in	IN
cord-014597-66vd2mdu	324	5	SCM	SCM	NNP
cord-014597-66vd2mdu	324	6	,	,	,
cord-014597-66vd2mdu	324	7	the	the	DT
cord-014597-66vd2mdu	324	8	residual	residual	JJ
cord-014597-66vd2mdu	324	9	protein	protein	NN
cord-014597-66vd2mdu	324	10	content	content	NN
cord-014597-66vd2mdu	324	11	was	be	VBD
cord-014597-66vd2mdu	324	12	slightly	slightly	RB
cord-014597-66vd2mdu	324	13	less	less	RBR
cord-014597-66vd2mdu	324	14	compared	compare	VBN
cord-014597-66vd2mdu	324	15	to	to	IN
cord-014597-66vd2mdu	324	16	OMV	omv	NN
cord-014597-66vd2mdu	324	17	present	present	JJ
cord-014597-66vd2mdu	324	18	in	in	IN
cord-014597-66vd2mdu	324	19	SFM	SFM	NNP
cord-014597-66vd2mdu	324	20	(	(	-LRB-
cord-014597-66vd2mdu	324	21	Table	table	NN
cord-014597-66vd2mdu	324	22	1	1	CD
cord-014597-66vd2mdu	324	23	)	)	-RRB-
cord-014597-66vd2mdu	324	24	.	.	.
cord-014597-66vd2mdu	325	1	In	in	IN
cord-014597-66vd2mdu	325	2	contrast	contrast	NN
cord-014597-66vd2mdu	325	3	,	,	,
cord-014597-66vd2mdu	325	4	host	host	NN
cord-014597-66vd2mdu	325	5	cell	cell	NN
cord-014597-66vd2mdu	325	6	DNA	dna	NN
cord-014597-66vd2mdu	325	7	(	(	-LRB-
cord-014597-66vd2mdu	325	8	hcDNA	hcdna	RB
cord-014597-66vd2mdu	325	9	)	)	-RRB-
cord-014597-66vd2mdu	325	10	was	be	VBD
cord-014597-66vd2mdu	325	11	bound	bind	VBN
cord-014597-66vd2mdu	325	12	to	to	IN
cord-014597-66vd2mdu	325	13	the	the	DT
cord-014597-66vd2mdu	325	14	filter	filter	NN
cord-014597-66vd2mdu	325	15	material	material	NN
cord-014597-66vd2mdu	325	16	.	.	.
cord-014597-66vd2mdu	326	1	We	-PRON-	PRP
cord-014597-66vd2mdu	326	2	achieved	achieve	VBD
cord-014597-66vd2mdu	326	3	a	a	DT
cord-014597-66vd2mdu	326	4	33	33	CD
cord-014597-66vd2mdu	326	5	%	%	NN
cord-014597-66vd2mdu	326	6	reduction	reduction	NN
cord-014597-66vd2mdu	326	7	of	of	IN
cord-014597-66vd2mdu	326	8	hcDNA	hcdna	NN
cord-014597-66vd2mdu	326	9	for	for	IN
cord-014597-66vd2mdu	326	10	an	an	DT
cord-014597-66vd2mdu	326	11	OMV	OMV	NNP
cord-014597-66vd2mdu	326	12	suspension	suspension	NN
cord-014597-66vd2mdu	326	13	in	in	IN
cord-014597-66vd2mdu	326	14	SFM	SFM	NNP
cord-014597-66vd2mdu	326	15	.	.	.
cord-014597-66vd2mdu	327	1	After	after	IN
cord-014597-66vd2mdu	327	2	clarifying	clarify	VBG
cord-014597-66vd2mdu	327	3	an	an	DT
cord-014597-66vd2mdu	327	4	OMV	omv	NN
cord-014597-66vd2mdu	327	5	suspension	suspension	NN
cord-014597-66vd2mdu	327	6	in	in	IN
cord-014597-66vd2mdu	327	7	SFM	SFM	NNP
cord-014597-66vd2mdu	327	8	,	,	,
cord-014597-66vd2mdu	327	9	the	the	DT
cord-014597-66vd2mdu	327	10	remaining	remain	VBG
cord-014597-66vd2mdu	327	11	hcDNA	hcdna	JJ
cord-014597-66vd2mdu	327	12	content	content	NN
cord-014597-66vd2mdu	327	13	was	be	VBD
cord-014597-66vd2mdu	327	14	even	even	RB
cord-014597-66vd2mdu	327	15	lower	low	JJR
cord-014597-66vd2mdu	327	16	being	be	VBG
cord-014597-66vd2mdu	327	17	only	only	RB
cord-014597-66vd2mdu	327	18	42	42	CD
cord-014597-66vd2mdu	327	19	%	%	NN
cord-014597-66vd2mdu	327	20	.	.	.
cord-014597-66vd2mdu	328	1	Conclusions	conclusion	NNS
cord-014597-66vd2mdu	328	2	Charged	charge	VBN
cord-014597-66vd2mdu	328	3	depth	depth	NN
cord-014597-66vd2mdu	328	4	filters	filter	NNS
cord-014597-66vd2mdu	328	5	are	be	VBP
cord-014597-66vd2mdu	328	6	suitable	suitable	JJ
cord-014597-66vd2mdu	328	7	for	for	IN
cord-014597-66vd2mdu	328	8	the	the	DT
cord-014597-66vd2mdu	328	9	first	first	JJ
cord-014597-66vd2mdu	328	10	clarification	clarification	NN
cord-014597-66vd2mdu	328	11	step	step	NN
cord-014597-66vd2mdu	328	12	of	of	IN
cord-014597-66vd2mdu	328	13	OMV	OMV	NNP
cord-014597-66vd2mdu	328	14	downstream	downstream	JJ
cord-014597-66vd2mdu	328	15	processing	processing	NN
cord-014597-66vd2mdu	328	16	.	.	.
cord-014597-66vd2mdu	329	1	Residual	residual	JJ
cord-014597-66vd2mdu	329	2	protein	protein	NN
cord-014597-66vd2mdu	329	3	could	could	MD
cord-014597-66vd2mdu	329	4	pass	pass	VB
cord-014597-66vd2mdu	329	5	the	the	DT
cord-014597-66vd2mdu	329	6	depth	depth	NN
cord-014597-66vd2mdu	329	7	Scheme	scheme	NN
cord-014597-66vd2mdu	329	8	of	of	IN
cord-014597-66vd2mdu	329	9	the	the	DT
cord-014597-66vd2mdu	329	10	complete	complete	JJ
cord-014597-66vd2mdu	329	11	cell	cell	NN
cord-014597-66vd2mdu	329	12	line	line	NN
cord-014597-66vd2mdu	329	13	development	development	NN
cord-014597-66vd2mdu	329	14	workflow	workflow	NN
cord-014597-66vd2mdu	329	15	support	support	NN
cord-014597-66vd2mdu	329	16	in	in	IN
cord-014597-66vd2mdu	329	17	Genedata	Genedata	NNP
cord-014597-66vd2mdu	329	18	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	329	19	.	.	.
cord-014597-66vd2mdu	330	1	Showcasing	showcase	VBG
cord-014597-66vd2mdu	330	2	integration	integration	NN
cord-014597-66vd2mdu	330	3	of	of	IN
cord-014597-66vd2mdu	330	4	data	datum	NNS
cord-014597-66vd2mdu	330	5	from	from	IN
cord-014597-66vd2mdu	330	6	diverse	diverse	JJ
cord-014597-66vd2mdu	330	7	measurement	measurement	NN
cord-014597-66vd2mdu	330	8	instruments	instrument	NNS
cord-014597-66vd2mdu	330	9	,	,	,
cord-014597-66vd2mdu	330	10	data	datum	NNS
cord-014597-66vd2mdu	330	11	visualization	visualization	NN
cord-014597-66vd2mdu	330	12	for	for	IN
cord-014597-66vd2mdu	330	13	decision	decision	NN
cord-014597-66vd2mdu	330	14	making	make	VBG
cord-014597-66vd2mdu	330	15	support	support	NN
cord-014597-66vd2mdu	330	16	as	as	RB
cord-014597-66vd2mdu	330	17	well	well	RB
cord-014597-66vd2mdu	330	18	as	as	IN
cord-014597-66vd2mdu	330	19	,	,	,
cord-014597-66vd2mdu	330	20	tracking	tracking	NN
cord-014597-66vd2mdu	330	21	of	of	IN
cord-014597-66vd2mdu	330	22	full	full	JJ
cord-014597-66vd2mdu	330	23	clone	clone	NN
cord-014597-66vd2mdu	330	24	history	history	NN
cord-014597-66vd2mdu	330	25	filter	filter	NN
cord-014597-66vd2mdu	330	26	almost	almost	RB
cord-014597-66vd2mdu	330	27	unhindered	unhindered	JJ
cord-014597-66vd2mdu	330	28	,	,	,
cord-014597-66vd2mdu	330	29	whereas	whereas	IN
cord-014597-66vd2mdu	330	30	the	the	DT
cord-014597-66vd2mdu	330	31	hcDNA	hcdna	JJ
cord-014597-66vd2mdu	330	32	content	content	NN
cord-014597-66vd2mdu	330	33	was	be	VBD
cord-014597-66vd2mdu	330	34	already	already	RB
cord-014597-66vd2mdu	330	35	reduced	reduce	VBN
cord-014597-66vd2mdu	330	36	to	to	IN
cord-014597-66vd2mdu	330	37	42	42	CD
cord-014597-66vd2mdu	330	38	%	%	NN
cord-014597-66vd2mdu	330	39	at	at	IN
cord-014597-66vd2mdu	330	40	maximum	maximum	NN
cord-014597-66vd2mdu	330	41	.	.	.
cord-014597-66vd2mdu	331	1	However	however	RB
cord-014597-66vd2mdu	331	2	,	,	,
cord-014597-66vd2mdu	331	3	the	the	DT
cord-014597-66vd2mdu	331	4	OMV	OMV	NNP
cord-014597-66vd2mdu	331	5	titer	titer	NN
cord-014597-66vd2mdu	331	6	was	be	VBD
cord-014597-66vd2mdu	331	7	also	also	RB
cord-014597-66vd2mdu	331	8	reduced	reduce	VBN
cord-014597-66vd2mdu	331	9	by	by	IN
cord-014597-66vd2mdu	331	10	the	the	DT
cord-014597-66vd2mdu	331	11	depth	depth	NN
cord-014597-66vd2mdu	331	12	filtration	filtration	NN
cord-014597-66vd2mdu	331	13	.	.	.
cord-014597-66vd2mdu	332	1	This	this	DT
cord-014597-66vd2mdu	332	2	undesired	undesired	JJ
cord-014597-66vd2mdu	332	3	effect	effect	NN
cord-014597-66vd2mdu	332	4	was	be	VBD
cord-014597-66vd2mdu	332	5	stronger	strong	JJR
cord-014597-66vd2mdu	332	6	for	for	IN
cord-014597-66vd2mdu	332	7	the	the	DT
cord-014597-66vd2mdu	332	8	OMV	OMV	NNP
cord-014597-66vd2mdu	332	9	present	present	NN
cord-014597-66vd2mdu	332	10	in	in	IN
cord-014597-66vd2mdu	332	11	SFM	SFM	NNP
cord-014597-66vd2mdu	332	12	.	.	.
cord-014597-66vd2mdu	333	1	Because	because	IN
cord-014597-66vd2mdu	333	2	the	the	DT
cord-014597-66vd2mdu	333	3	agencies	agency	NNS
cord-014597-66vd2mdu	333	4	require	require	VBP
cord-014597-66vd2mdu	333	5	avoiding	avoid	VBG
cord-014597-66vd2mdu	333	6	serum	serum	NN
cord-014597-66vd2mdu	333	7	in	in	IN
cord-014597-66vd2mdu	333	8	clinical	clinical	JJ
cord-014597-66vd2mdu	333	9	-	-	HYPH
cord-014597-66vd2mdu	333	10	grade	grade	NN
cord-014597-66vd2mdu	333	11	production	production	NN
cord-014597-66vd2mdu	333	12	processes	process	NNS
cord-014597-66vd2mdu	333	13	,	,	,
cord-014597-66vd2mdu	333	14	this	this	DT
cord-014597-66vd2mdu	333	15	is	be	VBZ
cord-014597-66vd2mdu	333	16	disadvantageous	disadvantageous	JJ
cord-014597-66vd2mdu	333	17	.	.	.
cord-014597-66vd2mdu	334	1	Nonetheless	nonetheless	RB
cord-014597-66vd2mdu	334	2	,	,	,
cord-014597-66vd2mdu	334	3	because	because	IN
cord-014597-66vd2mdu	334	4	SFM	SFM	NNP
cord-014597-66vd2mdu	334	5	will	will	MD
cord-014597-66vd2mdu	334	6	be	be	VB
cord-014597-66vd2mdu	334	7	soon	soon	RB
cord-014597-66vd2mdu	334	8	standard	standard	JJ
cord-014597-66vd2mdu	334	9	for	for	IN
cord-014597-66vd2mdu	334	10	OMV	OMV	NNP
cord-014597-66vd2mdu	334	11	production	production	NN
cord-014597-66vd2mdu	334	12	,	,	,
cord-014597-66vd2mdu	334	13	further	further	JJ
cord-014597-66vd2mdu	334	14	experiments	experiment	NNS
cord-014597-66vd2mdu	334	15	have	have	VBP
cord-014597-66vd2mdu	334	16	to	to	TO
cord-014597-66vd2mdu	334	17	be	be	VB
cord-014597-66vd2mdu	334	18	done	do	VBN
cord-014597-66vd2mdu	334	19	preventing	prevent	VBG
cord-014597-66vd2mdu	334	20	the	the	DT
cord-014597-66vd2mdu	334	21	OMV	OMV	NNP
cord-014597-66vd2mdu	334	22	reduction	reduction	NN
cord-014597-66vd2mdu	334	23	during	during	IN
cord-014597-66vd2mdu	334	24	clarification	clarification	NN
cord-014597-66vd2mdu	334	25	.	.	.
cord-014597-66vd2mdu	335	1	One	one	CD
cord-014597-66vd2mdu	335	2	option	option	NN
cord-014597-66vd2mdu	335	3	can	can	MD
cord-014597-66vd2mdu	335	4	be	be	VB
cord-014597-66vd2mdu	335	5	to	to	TO
cord-014597-66vd2mdu	335	6	reduce	reduce	VB
cord-014597-66vd2mdu	335	7	the	the	DT
cord-014597-66vd2mdu	335	8	adsorption	adsorption	NN
cord-014597-66vd2mdu	335	9	strength	strength	NN
cord-014597-66vd2mdu	335	10	of	of	IN
cord-014597-66vd2mdu	335	11	the	the	DT
cord-014597-66vd2mdu	335	12	virus	virus	NN
cord-014597-66vd2mdu	335	13	to	to	IN
cord-014597-66vd2mdu	335	14	the	the	DT
cord-014597-66vd2mdu	335	15	filter	filter	NN
cord-014597-66vd2mdu	335	16	material	material	NN
cord-014597-66vd2mdu	335	17	by	by	IN
cord-014597-66vd2mdu	335	18	the	the	DT
cord-014597-66vd2mdu	335	19	addition	addition	NN
cord-014597-66vd2mdu	335	20	of	of	IN
cord-014597-66vd2mdu	335	21	salt	salt	NN
cord-014597-66vd2mdu	335	22	.	.	.
cord-014597-66vd2mdu	336	1	Moreover	moreover	RB
cord-014597-66vd2mdu	336	2	,	,	,
cord-014597-66vd2mdu	336	3	it	-PRON-	PRP
cord-014597-66vd2mdu	336	4	is	be	VBZ
cord-014597-66vd2mdu	336	5	important	important	JJ
cord-014597-66vd2mdu	336	6	to	to	TO
cord-014597-66vd2mdu	336	7	establish	establish	VB
cord-014597-66vd2mdu	336	8	a	a	DT
cord-014597-66vd2mdu	336	9	standardized	standardized	JJ
cord-014597-66vd2mdu	336	10	protocol	protocol	NN
cord-014597-66vd2mdu	336	11	for	for	IN
cord-014597-66vd2mdu	336	12	the	the	DT
cord-014597-66vd2mdu	336	13	upstream	upstream	JJ
cord-014597-66vd2mdu	336	14	processing	processing	NN
cord-014597-66vd2mdu	336	15	.	.	.
cord-014597-66vd2mdu	337	1	We	-PRON-	PRP
cord-014597-66vd2mdu	337	2	determined	determine	VBD
cord-014597-66vd2mdu	337	3	batch	batch	NN
cord-014597-66vd2mdu	337	4	-	-	HYPH
cord-014597-66vd2mdu	337	5	to	to	IN
cord-014597-66vd2mdu	337	6	-	-	HYPH
cord-014597-66vd2mdu	337	7	batch	batch	NN
cord-014597-66vd2mdu	337	8	variations	variation	NNS
cord-014597-66vd2mdu	337	9	within	within	IN
cord-014597-66vd2mdu	337	10	the	the	DT
cord-014597-66vd2mdu	337	11	clarification	clarification	NN
cord-014597-66vd2mdu	337	12	indicating	indicate	VBG
cord-014597-66vd2mdu	337	13	a	a	DT
cord-014597-66vd2mdu	337	14	strong	strong	JJ
cord-014597-66vd2mdu	337	15	impact	impact	NN
cord-014597-66vd2mdu	337	16	of	of	IN
cord-014597-66vd2mdu	337	17	upstream	upstream	JJ
cord-014597-66vd2mdu	337	18	processing	processing	NN
cord-014597-66vd2mdu	337	19	(	(	-LRB-
cord-014597-66vd2mdu	337	20	USP	USP	NNP
cord-014597-66vd2mdu	337	21	)	)	-RRB-
cord-014597-66vd2mdu	337	22	on	on	IN
cord-014597-66vd2mdu	337	23	the	the	DT
cord-014597-66vd2mdu	337	24	outcome	outcome	NN
cord-014597-66vd2mdu	337	25	of	of	IN
cord-014597-66vd2mdu	337	26	the	the	DT
cord-014597-66vd2mdu	337	27	DSP	DSP	NNP
cord-014597-66vd2mdu	337	28	.	.	.
cord-014597-66vd2mdu	338	1	Therefore	therefore	RB
cord-014597-66vd2mdu	338	2	,	,	,
cord-014597-66vd2mdu	338	3	further	further	JJ
cord-014597-66vd2mdu	338	4	studies	study	NNS
cord-014597-66vd2mdu	338	5	must	must	MD
cord-014597-66vd2mdu	338	6	investigate	investigate	VB
cord-014597-66vd2mdu	338	7	the	the	DT
cord-014597-66vd2mdu	338	8	influence	influence	NN
cord-014597-66vd2mdu	338	9	of	of	IN
cord-014597-66vd2mdu	338	10	USP	USP	NNP
cord-014597-66vd2mdu	338	11	parameter	parameter	NN
cord-014597-66vd2mdu	338	12	e.g.	e.g.	IN
cord-014597-66vd2mdu	338	13	time	time	NN
cord-014597-66vd2mdu	338	14	of	of	IN
cord-014597-66vd2mdu	338	15	harvest	harvest	NN
cord-014597-66vd2mdu	338	16	and	and	CC
cord-014597-66vd2mdu	338	17	pH	ph	NN
cord-014597-66vd2mdu	338	18	of	of	IN
cord-014597-66vd2mdu	338	19	the	the	DT
cord-014597-66vd2mdu	338	20	harvest	harvest	NN
cord-014597-66vd2mdu	338	21	solution	solution	NN
cord-014597-66vd2mdu	338	22	on	on	IN
cord-014597-66vd2mdu	338	23	the	the	DT
cord-014597-66vd2mdu	338	24	OMV	OMV	NNP
cord-014597-66vd2mdu	338	25	.	.	.
cord-014597-66vd2mdu	339	1	Fed	Fed	NNP
cord-014597-66vd2mdu	339	2	-	-	HYPH
cord-014597-66vd2mdu	339	3	batch	batch	NN
cord-014597-66vd2mdu	339	4	culture	culture	NN
cord-014597-66vd2mdu	339	5	is	be	VBZ
cord-014597-66vd2mdu	339	6	commonly	commonly	RB
cord-014597-66vd2mdu	339	7	employed	employ	VBN
cord-014597-66vd2mdu	339	8	to	to	TO
cord-014597-66vd2mdu	339	9	maximize	maximize	VB
cord-014597-66vd2mdu	339	10	cell	cell	NN
cord-014597-66vd2mdu	339	11	and	and	CC
cord-014597-66vd2mdu	339	12	product	product	NN
cord-014597-66vd2mdu	339	13	concentrations	concentration	NNS
cord-014597-66vd2mdu	339	14	in	in	IN
cord-014597-66vd2mdu	339	15	upstream	upstream	JJ
cord-014597-66vd2mdu	339	16	mammalian	mammalian	JJ
cord-014597-66vd2mdu	339	17	cell	cell	NN
cord-014597-66vd2mdu	339	18	culture	culture	NN
cord-014597-66vd2mdu	339	19	processes	process	NNS
cord-014597-66vd2mdu	339	20	.	.	.
cord-014597-66vd2mdu	340	1	Typical	typical	JJ
cord-014597-66vd2mdu	340	2	standard	standard	JJ
cord-014597-66vd2mdu	340	3	platform	platform	NN
cord-014597-66vd2mdu	340	4	processes	process	NNS
cord-014597-66vd2mdu	340	5	rely	rely	VBP
cord-014597-66vd2mdu	340	6	on	on	IN
cord-014597-66vd2mdu	340	7	fixed	fix	VBN
cord-014597-66vd2mdu	340	8	-	-	HYPH
cord-014597-66vd2mdu	340	9	volume	volume	NN
cord-014597-66vd2mdu	340	10	bolus	bolus	NN
cord-014597-66vd2mdu	340	11	feeding	feeding	NN
cord-014597-66vd2mdu	340	12	of	of	IN
cord-014597-66vd2mdu	340	13	concentrated	concentrated	JJ
cord-014597-66vd2mdu	340	14	feed	feed	NN
cord-014597-66vd2mdu	340	15	supplements	supplement	NNS
cord-014597-66vd2mdu	340	16	at	at	IN
cord-014597-66vd2mdu	340	17	regular	regular	JJ
cord-014597-66vd2mdu	340	18	intervals	interval	NNS
cord-014597-66vd2mdu	340	19	.	.	.
cord-014597-66vd2mdu	341	1	However	however	RB
cord-014597-66vd2mdu	341	2	,	,	,
cord-014597-66vd2mdu	341	3	such	such	JJ
cord-014597-66vd2mdu	341	4	static	static	JJ
cord-014597-66vd2mdu	341	5	approaches	approach	NNS
cord-014597-66vd2mdu	341	6	might	may	MD
cord-014597-66vd2mdu	341	7	result	result	VB
cord-014597-66vd2mdu	341	8	in	in	IN
cord-014597-66vd2mdu	341	9	over	over	IN
cord-014597-66vd2mdu	341	10	-	-	HYPH
cord-014597-66vd2mdu	341	11	or	or	CC
cord-014597-66vd2mdu	341	12	underfeeding	underfeeding	NN
cord-014597-66vd2mdu	341	13	.	.	.
cord-014597-66vd2mdu	342	1	To	to	TO
cord-014597-66vd2mdu	342	2	mimic	mimic	VB
cord-014597-66vd2mdu	342	3	more	more	RBR
cord-014597-66vd2mdu	342	4	closely	closely	RB
cord-014597-66vd2mdu	342	5	the	the	DT
cord-014597-66vd2mdu	342	6	dynamics	dynamic	NNS
cord-014597-66vd2mdu	342	7	of	of	IN
cord-014597-66vd2mdu	342	8	a	a	DT
cord-014597-66vd2mdu	342	9	fed	feed	VBN
cord-014597-66vd2mdu	342	10	-	-	HYPH
cord-014597-66vd2mdu	342	11	batch	batch	NN
cord-014597-66vd2mdu	342	12	culture	culture	NN
cord-014597-66vd2mdu	342	13	,	,	,
cord-014597-66vd2mdu	342	14	we	-PRON-	PRP
cord-014597-66vd2mdu	342	15	developed	develop	VBD
cord-014597-66vd2mdu	342	16	a	a	DT
cord-014597-66vd2mdu	342	17	dynamic	dynamic	JJ
cord-014597-66vd2mdu	342	18	feeding	feeding	NN
cord-014597-66vd2mdu	342	19	strategy	strategy	NN
cord-014597-66vd2mdu	342	20	responsive	responsive	JJ
cord-014597-66vd2mdu	342	21	to	to	IN
cord-014597-66vd2mdu	342	22	the	the	DT
cord-014597-66vd2mdu	342	23	actual	actual	JJ
cord-014597-66vd2mdu	342	24	nutrient	nutrient	NN
cord-014597-66vd2mdu	342	25	needs	need	NNS
cord-014597-66vd2mdu	342	26	of	of	IN
cord-014597-66vd2mdu	342	27	a	a	DT
cord-014597-66vd2mdu	342	28	mAb	mab	NN
cord-014597-66vd2mdu	342	29	-	-	HYPH
cord-014597-66vd2mdu	342	30	producing	produce	VBG
cord-014597-66vd2mdu	342	31	recombinant	recombinant	JJ
cord-014597-66vd2mdu	342	32	CHO	CHO	NNP
cord-014597-66vd2mdu	342	33	cell	cell	NN
cord-014597-66vd2mdu	342	34	line	line	NN
cord-014597-66vd2mdu	342	35	.	.	.
cord-014597-66vd2mdu	343	1	Results	result	NNS
cord-014597-66vd2mdu	343	2	and	and	CC
cord-014597-66vd2mdu	343	3	discussion	discussion	NN
cord-014597-66vd2mdu	343	4	Improvements	improvement	NNS
cord-014597-66vd2mdu	343	5	made	make	VBN
cord-014597-66vd2mdu	343	6	at	at	IN
cord-014597-66vd2mdu	343	7	different	different	JJ
cord-014597-66vd2mdu	343	8	steps	step	NNS
cord-014597-66vd2mdu	343	9	during	during	IN
cord-014597-66vd2mdu	343	10	fed	fed	NNP
cord-014597-66vd2mdu	343	11	-	-	HYPH
cord-014597-66vd2mdu	343	12	batch	batch	NN
cord-014597-66vd2mdu	343	13	development	development	NN
cord-014597-66vd2mdu	343	14	are	be	VBP
cord-014597-66vd2mdu	343	15	shown	show	VBN
cord-014597-66vd2mdu	343	16	in	in	IN
cord-014597-66vd2mdu	343	17	Fig	Fig	NNP
cord-014597-66vd2mdu	343	18	.	.	.
cord-014597-66vd2mdu	344	1	1	1	LS
cord-014597-66vd2mdu	344	2	.	.	.
cord-014597-66vd2mdu	345	1	At	at	IN
cord-014597-66vd2mdu	345	2	step	step	NN
cord-014597-66vd2mdu	345	3	1	1	CD
cord-014597-66vd2mdu	345	4	,	,	,
cord-014597-66vd2mdu	345	5	all	all	DT
cord-014597-66vd2mdu	345	6	eight	eight	CD
cord-014597-66vd2mdu	345	7	Cell	cell	NN
cord-014597-66vd2mdu	345	8	Boost	boost	NN
cord-014597-66vd2mdu	345	9	supplements	supplement	NNS
cord-014597-66vd2mdu	345	10	were	be	VBD
cord-014597-66vd2mdu	345	11	added	add	VBN
cord-014597-66vd2mdu	345	12	to	to	IN
cord-014597-66vd2mdu	345	13	CDM4NS0	cdm4ns0	NN
cord-014597-66vd2mdu	345	14	according	accord	VBG
cord-014597-66vd2mdu	345	15	to	to	IN
cord-014597-66vd2mdu	345	16	a	a	DT
cord-014597-66vd2mdu	345	17	DoE	doe	NN
cord-014597-66vd2mdu	345	18	approach	approach	NN
cord-014597-66vd2mdu	345	19	,	,	,
cord-014597-66vd2mdu	345	20	and	and	CC
cord-014597-66vd2mdu	345	21	batch	batch	NN
cord-014597-66vd2mdu	345	22	cultures	culture	NNS
cord-014597-66vd2mdu	345	23	were	be	VBD
cord-014597-66vd2mdu	345	24	performed	perform	VBN
cord-014597-66vd2mdu	345	25	.	.	.
cord-014597-66vd2mdu	346	1	This	this	DT
cord-014597-66vd2mdu	346	2	evaluation	evaluation	NN
cord-014597-66vd2mdu	346	3	allowed	allow	VBD
cord-014597-66vd2mdu	346	4	us	-PRON-	PRP
cord-014597-66vd2mdu	346	5	to	to	TO
cord-014597-66vd2mdu	346	6	select	select	VB
cord-014597-66vd2mdu	346	7	only	only	RB
cord-014597-66vd2mdu	346	8	those	those	DT
cord-014597-66vd2mdu	346	9	Cell	cell	NN
cord-014597-66vd2mdu	346	10	Boost	boost	NN
cord-014597-66vd2mdu	346	11	supplements	supplement	NNS
cord-014597-66vd2mdu	346	12	that	that	WDT
cord-014597-66vd2mdu	346	13	were	be	VBD
cord-014597-66vd2mdu	346	14	beneficial	beneficial	JJ
cord-014597-66vd2mdu	346	15	to	to	IN
cord-014597-66vd2mdu	346	16	the	the	DT
cord-014597-66vd2mdu	346	17	overall	overall	JJ
cord-014597-66vd2mdu	346	18	culture	culture	NN
cord-014597-66vd2mdu	346	19	performance	performance	NN
cord-014597-66vd2mdu	346	20	.	.	.
cord-014597-66vd2mdu	347	1	Non	non	JJ
cord-014597-66vd2mdu	347	2	-	-	JJ
cord-014597-66vd2mdu	347	3	performing	perform	VBG
cord-014597-66vd2mdu	347	4	Cell	cell	NN
cord-014597-66vd2mdu	347	5	Boost	boost	NN
cord-014597-66vd2mdu	347	6	supplements	supplement	NNS
cord-014597-66vd2mdu	347	7	were	be	VBD
cord-014597-66vd2mdu	347	8	removed	remove	VBN
cord-014597-66vd2mdu	347	9	and	and	CC
cord-014597-66vd2mdu	347	10	not	not	RB
cord-014597-66vd2mdu	347	11	considered	consider	VBN
cord-014597-66vd2mdu	347	12	further	far	RBR
cord-014597-66vd2mdu	347	13	.	.	.
cord-014597-66vd2mdu	348	1	At	at	IN
cord-014597-66vd2mdu	348	2	step	step	NN
cord-014597-66vd2mdu	348	3	2	2	CD
cord-014597-66vd2mdu	348	4	,	,	,
cord-014597-66vd2mdu	348	5	the	the	DT
cord-014597-66vd2mdu	348	6	selected	select	VBN
cord-014597-66vd2mdu	348	7	Cell	Cell	NNP
cord-014597-66vd2mdu	348	8	Boost	Boost	NNP
cord-014597-66vd2mdu	348	9	supplements	supplement	NNS
cord-014597-66vd2mdu	348	10	were	be	VBD
cord-014597-66vd2mdu	348	11	added	add	VBN
cord-014597-66vd2mdu	348	12	daily	daily	RB
cord-014597-66vd2mdu	348	13	to	to	IN
cord-014597-66vd2mdu	348	14	the	the	DT
cord-014597-66vd2mdu	348	15	cultures	culture	NNS
cord-014597-66vd2mdu	348	16	at	at	IN
cord-014597-66vd2mdu	348	17	different	different	JJ
cord-014597-66vd2mdu	348	18	ratios	ratio	NNS
cord-014597-66vd2mdu	348	19	according	accord	VBG
cord-014597-66vd2mdu	348	20	to	to	IN
cord-014597-66vd2mdu	348	21	a	a	DT
cord-014597-66vd2mdu	348	22	DoE	doe	NN
cord-014597-66vd2mdu	348	23	approach	approach	NN
cord-014597-66vd2mdu	348	24	,	,	,
cord-014597-66vd2mdu	348	25	and	and	CC
cord-014597-66vd2mdu	348	26	fedbatch	fedbatch	NN
cord-014597-66vd2mdu	348	27	cultures	culture	NNS
cord-014597-66vd2mdu	348	28	were	be	VBD
cord-014597-66vd2mdu	348	29	conducted	conduct	VBN
cord-014597-66vd2mdu	348	30	.	.	.
cord-014597-66vd2mdu	349	1	As	as	IN
cord-014597-66vd2mdu	349	2	expected	expect	VBN
cord-014597-66vd2mdu	349	3	,	,	,
cord-014597-66vd2mdu	349	4	daily	daily	JJ
cord-014597-66vd2mdu	349	5	feed	feed	NN
cord-014597-66vd2mdu	349	6	additions	addition	NNS
cord-014597-66vd2mdu	349	7	to	to	TO
cord-014597-66vd2mdu	349	8	replenish	replenish	VB
cord-014597-66vd2mdu	349	9	consumed	consume	VBN
cord-014597-66vd2mdu	349	10	nutrients	nutrient	NNS
cord-014597-66vd2mdu	349	11	substantially	substantially	RB
cord-014597-66vd2mdu	349	12	improved	improve	VBN
cord-014597-66vd2mdu	349	13	mAb	mab	NN
cord-014597-66vd2mdu	349	14	and	and	CC
cord-014597-66vd2mdu	349	15	peak	peak	NN
cord-014597-66vd2mdu	349	16	cell	cell	NN
cord-014597-66vd2mdu	349	17	concentrations	concentration	NNS
cord-014597-66vd2mdu	349	18	as	as	RB
cord-014597-66vd2mdu	349	19	well	well	RB
cord-014597-66vd2mdu	349	20	as	as	IN
cord-014597-66vd2mdu	349	21	viable	viable	JJ
cord-014597-66vd2mdu	349	22	cumulative	cumulative	JJ
cord-014597-66vd2mdu	349	23	cell	cell	NN
cord-014597-66vd2mdu	349	24	days	day	NNS
cord-014597-66vd2mdu	349	25	(	(	-LRB-
cord-014597-66vd2mdu	349	26	VCCD	VCCD	NNP
cord-014597-66vd2mdu	349	27	)	)	-RRB-
cord-014597-66vd2mdu	349	28	compared	compare	VBN
cord-014597-66vd2mdu	349	29	to	to	IN
cord-014597-66vd2mdu	349	30	batch	batch	VB
cord-014597-66vd2mdu	349	31	cultivation	cultivation	NN
cord-014597-66vd2mdu	349	32	.	.	.
cord-014597-66vd2mdu	350	1	Further	further	RB
cord-014597-66vd2mdu	350	2	,	,	,
cord-014597-66vd2mdu	350	3	the	the	DT
cord-014597-66vd2mdu	350	4	results	result	NNS
cord-014597-66vd2mdu	350	5	enabled	enable	VBD
cord-014597-66vd2mdu	350	6	us	-PRON-	PRP
cord-014597-66vd2mdu	350	7	to	to	IN
cord-014597-66vd2mdu	350	8	fine	fine	JJ
cord-014597-66vd2mdu	350	9	-	-	HYPH
cord-014597-66vd2mdu	350	10	tune	tune	VB
cord-014597-66vd2mdu	350	11	the	the	DT
cord-014597-66vd2mdu	350	12	feed	feed	NN
cord-014597-66vd2mdu	350	13	ratio	ratio	NN
cord-014597-66vd2mdu	350	14	of	of	IN
cord-014597-66vd2mdu	350	15	selected	select	VBN
cord-014597-66vd2mdu	350	16	Cell	Cell	NNP
cord-014597-66vd2mdu	350	17	Boost	Boost	NNP
cord-014597-66vd2mdu	350	18	supplements	supplement	NNS
cord-014597-66vd2mdu	350	19	.	.	.
cord-014597-66vd2mdu	351	1	At	at	IN
cord-014597-66vd2mdu	351	2	step	step	NN
cord-014597-66vd2mdu	351	3	3	3	CD
cord-014597-66vd2mdu	351	4	,	,	,
cord-014597-66vd2mdu	351	5	we	-PRON-	PRP
cord-014597-66vd2mdu	351	6	further	further	RB
cord-014597-66vd2mdu	351	7	optimized	optimize	VBD
cord-014597-66vd2mdu	351	8	the	the	DT
cord-014597-66vd2mdu	351	9	best	good	JJS
cord-014597-66vd2mdu	351	10	performing	perform	VBG
cord-014597-66vd2mdu	351	11	feed	feed	NN
cord-014597-66vd2mdu	351	12	ratio	ratio	NN
cord-014597-66vd2mdu	351	13	by	by	IN
cord-014597-66vd2mdu	351	14	investigation	investigation	NN
cord-014597-66vd2mdu	351	15	of	of	IN
cord-014597-66vd2mdu	351	16	static	static	JJ
cord-014597-66vd2mdu	351	17	and	and	CC
cord-014597-66vd2mdu	351	18	dynamic	dynamic	JJ
cord-014597-66vd2mdu	351	19	feed	feed	NN
cord-014597-66vd2mdu	351	20	protocols	protocol	NNS
cord-014597-66vd2mdu	351	21	.	.	.
cord-014597-66vd2mdu	352	1	Most	Most	JJS
cord-014597-66vd2mdu	352	2	fed	fed	JJ
cord-014597-66vd2mdu	352	3	-	-	HYPH
cord-014597-66vd2mdu	352	4	batch	batch	NN
cord-014597-66vd2mdu	352	5	protocols	protocol	NNS
cord-014597-66vd2mdu	352	6	rely	rely	VBP
cord-014597-66vd2mdu	352	7	on	on	IN
cord-014597-66vd2mdu	352	8	constant	constant	JJ
cord-014597-66vd2mdu	352	9	feed	feed	NN
cord-014597-66vd2mdu	352	10	additions	addition	NNS
cord-014597-66vd2mdu	352	11	on	on	IN
cord-014597-66vd2mdu	352	12	distinct	distinct	JJ
cord-014597-66vd2mdu	352	13	days	day	NNS
cord-014597-66vd2mdu	352	14	.	.	.
cord-014597-66vd2mdu	353	1	However	however	RB
cord-014597-66vd2mdu	353	2	,	,	,
cord-014597-66vd2mdu	353	3	these	these	DT
cord-014597-66vd2mdu	353	4	approaches	approach	NNS
cord-014597-66vd2mdu	353	5	often	often	RB
cord-014597-66vd2mdu	353	6	lead	lead	VBP
cord-014597-66vd2mdu	353	7	to	to	IN
cord-014597-66vd2mdu	353	8	substantial	substantial	JJ
cord-014597-66vd2mdu	353	9	over	over	RB
cord-014597-66vd2mdu	353	10	-	-	HYPH
cord-014597-66vd2mdu	353	11	or	or	CC
cord-014597-66vd2mdu	353	12	underfeeding	underfeede	VBG
cord-014597-66vd2mdu	353	13	during	during	IN
cord-014597-66vd2mdu	353	14	bioprocessing	bioprocessing	NN
cord-014597-66vd2mdu	353	15	.	.	.
cord-014597-66vd2mdu	354	1	To	to	TO
cord-014597-66vd2mdu	354	2	improve	improve	VB
cord-014597-66vd2mdu	354	3	such	such	JJ
cord-014597-66vd2mdu	354	4	"	"	``
cord-014597-66vd2mdu	354	5	static	static	JJ
cord-014597-66vd2mdu	354	6	"	"	''
cord-014597-66vd2mdu	354	7	protocols	protocol	NNS
cord-014597-66vd2mdu	354	8	,	,	,
cord-014597-66vd2mdu	354	9	we	-PRON-	PRP
cord-014597-66vd2mdu	354	10	investigated	investigate	VBD
cord-014597-66vd2mdu	354	11	three	three	CD
cord-014597-66vd2mdu	354	12	different	different	JJ
cord-014597-66vd2mdu	354	13	"	"	``
cord-014597-66vd2mdu	354	14	dynamic	dynamic	JJ
cord-014597-66vd2mdu	354	15	"	"	''
cord-014597-66vd2mdu	354	16	approaches	approach	NNS
cord-014597-66vd2mdu	354	17	as	as	IN
cord-014597-66vd2mdu	354	18	shown	show	VBN
cord-014597-66vd2mdu	354	19	in	in	IN
cord-014597-66vd2mdu	354	20	Table	Table	NNP
cord-014597-66vd2mdu	354	21	1	1	CD
cord-014597-66vd2mdu	354	22	by	by	IN
cord-014597-66vd2mdu	354	23	applying	apply	VBG
cord-014597-66vd2mdu	354	24	the	the	DT
cord-014597-66vd2mdu	354	25	selected	select	VBN
cord-014597-66vd2mdu	354	26	Cell	Cell	NNP
cord-014597-66vd2mdu	354	27	Boost	Boost	NNP
cord-014597-66vd2mdu	354	28	supplements	supplement	NNS
cord-014597-66vd2mdu	354	29	with	with	IN
cord-014597-66vd2mdu	354	30	the	the	DT
cord-014597-66vd2mdu	354	31	optimized	optimize	VBN
cord-014597-66vd2mdu	354	32	feed	feed	NN
cord-014597-66vd2mdu	354	33	ratio	ratio	NN
cord-014597-66vd2mdu	354	34	.	.	.
cord-014597-66vd2mdu	355	1	This	this	DT
cord-014597-66vd2mdu	355	2	investigation	investigation	NN
cord-014597-66vd2mdu	355	3	allowed	allow	VBD
cord-014597-66vd2mdu	355	4	us	-PRON-	PRP
cord-014597-66vd2mdu	355	5	to	to	TO
cord-014597-66vd2mdu	355	6	further	further	RB
cord-014597-66vd2mdu	355	7	improve	improve	VB
cord-014597-66vd2mdu	355	8	the	the	DT
cord-014597-66vd2mdu	355	9	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	355	10	performance	performance	NN
cord-014597-66vd2mdu	355	11	.	.	.
cord-014597-66vd2mdu	356	1	The	the	DT
cord-014597-66vd2mdu	356	2	best	good	JJS
cord-014597-66vd2mdu	356	3	performing	perform	VBG
cord-014597-66vd2mdu	356	4	approaches	approach	NNS
cord-014597-66vd2mdu	356	5	,	,	,
cord-014597-66vd2mdu	356	6	constant	constant	JJ
cord-014597-66vd2mdu	356	7	and	and	CC
cord-014597-66vd2mdu	356	8	retrospective	retrospective	JJ
cord-014597-66vd2mdu	356	9	feed	feed	NN
cord-014597-66vd2mdu	356	10	,	,	,
cord-014597-66vd2mdu	356	11	were	be	VBD
cord-014597-66vd2mdu	356	12	further	further	RB
cord-014597-66vd2mdu	356	13	investigated	investigate	VBN
cord-014597-66vd2mdu	356	14	in	in	IN
cord-014597-66vd2mdu	356	15	fully	fully	RB
cord-014597-66vd2mdu	356	16	automated	automate	VBN
cord-014597-66vd2mdu	356	17	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	356	18	under	under	IN
cord-014597-66vd2mdu	356	19	controlled	control	VBN
cord-014597-66vd2mdu	356	20	conditions	condition	NNS
cord-014597-66vd2mdu	356	21	.	.	.
cord-014597-66vd2mdu	357	1	In	in	IN
cord-014597-66vd2mdu	357	2	general	general	JJ
cord-014597-66vd2mdu	357	3	,	,	,
cord-014597-66vd2mdu	357	4	constant	constant	JJ
cord-014597-66vd2mdu	357	5	cultivation	cultivation	NN
cord-014597-66vd2mdu	357	6	parameters	parameter	NNS
cord-014597-66vd2mdu	357	7	in	in	IN
cord-014597-66vd2mdu	357	8	the	the	DT
cord-014597-66vd2mdu	357	9	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	357	10	slightly	slightly	RB
cord-014597-66vd2mdu	357	11	enhanced	enhance	VBN
cord-014597-66vd2mdu	357	12	mAb	mAb	NNS
cord-014597-66vd2mdu	357	13	titers	titer	NNS
cord-014597-66vd2mdu	357	14	compared	compare	VBN
cord-014597-66vd2mdu	357	15	to	to	TO
cord-014597-66vd2mdu	357	16	shake	shake	VB
cord-014597-66vd2mdu	357	17	flask	flask	NN
cord-014597-66vd2mdu	357	18	cultivation	cultivation	NN
cord-014597-66vd2mdu	357	19	.	.	.
cord-014597-66vd2mdu	358	1	The	the	DT
cord-014597-66vd2mdu	358	2	retrospective	retrospective	JJ
cord-014597-66vd2mdu	358	3	feed	feed	NN
cord-014597-66vd2mdu	358	4	strategy	strategy	NN
cord-014597-66vd2mdu	358	5	yielded	yield	VBD
cord-014597-66vd2mdu	358	6	10	10	CD
cord-014597-66vd2mdu	358	7	%	%	NN
cord-014597-66vd2mdu	358	8	higher	high	JJR
cord-014597-66vd2mdu	358	9	titers	titer	NNS
cord-014597-66vd2mdu	358	10	than	than	IN
cord-014597-66vd2mdu	358	11	the	the	DT
cord-014597-66vd2mdu	358	12	constant	constant	JJ
cord-014597-66vd2mdu	358	13	strategy	strategy	NN
cord-014597-66vd2mdu	358	14	.	.	.
cord-014597-66vd2mdu	359	1	Overall	overall	RB
cord-014597-66vd2mdu	359	2	,	,	,
cord-014597-66vd2mdu	359	3	the	the	DT
cord-014597-66vd2mdu	359	4	established	establish	VBN
cord-014597-66vd2mdu	359	5	methodology	methodology	NN
cord-014597-66vd2mdu	359	6	for	for	IN
cord-014597-66vd2mdu	359	7	fed	fed	JJ
cord-014597-66vd2mdu	359	8	-	-	HYPH
cord-014597-66vd2mdu	359	9	batch	batch	NN
cord-014597-66vd2mdu	359	10	development	development	NN
cord-014597-66vd2mdu	359	11	allowed	allow	VBD
cord-014597-66vd2mdu	359	12	us	-PRON-	PRP
cord-014597-66vd2mdu	359	13	to	to	TO
cord-014597-66vd2mdu	359	14	obtain	obtain	VB
cord-014597-66vd2mdu	359	15	2.5×	2.5×	CD
cord-014597-66vd2mdu	359	16	higher	high	JJR
cord-014597-66vd2mdu	359	17	mAb	mAb	NNS
cord-014597-66vd2mdu	359	18	titers	titer	NNS
cord-014597-66vd2mdu	359	19	(	(	-LRB-
cord-014597-66vd2mdu	359	20	batch	batch	JJ
cord-014597-66vd2mdu	359	21	mean	mean	NN
cord-014597-66vd2mdu	359	22	:	:	:
cord-014597-66vd2mdu	359	23	1.9	1.9	CD
cord-014597-66vd2mdu	359	24	g	g	NN
cord-014597-66vd2mdu	359	25	/	/	SYM
cord-014597-66vd2mdu	359	26	L	l	NN
cord-014597-66vd2mdu	359	27	vs.	vs.	FW
cord-014597-66vd2mdu	359	28	fed	fed	JJ
cord-014597-66vd2mdu	359	29	-	-	HYPH
cord-014597-66vd2mdu	359	30	batch	batch	NN
cord-014597-66vd2mdu	359	31	4.9	4.9	CD
cord-014597-66vd2mdu	359	32	g	g	NN
cord-014597-66vd2mdu	359	33	/	/	SYM
cord-014597-66vd2mdu	359	34	L	L	NNP
cord-014597-66vd2mdu	359	35	)	)	-RRB-
cord-014597-66vd2mdu	359	36	in	in	IN
cord-014597-66vd2mdu	359	37	a	a	DT
cord-014597-66vd2mdu	359	38	short	short	JJ
cord-014597-66vd2mdu	359	39	time	time	NN
cord-014597-66vd2mdu	359	40	and	and	CC
cord-014597-66vd2mdu	359	41	three	three	CD
cord-014597-66vd2mdu	359	42	simple	simple	JJ
cord-014597-66vd2mdu	359	43	steps	step	NNS
cord-014597-66vd2mdu	359	44	.	.	.
cord-014597-66vd2mdu	360	1	In	in	IN
cord-014597-66vd2mdu	360	2	addition	addition	NN
cord-014597-66vd2mdu	360	3	,	,	,
cord-014597-66vd2mdu	360	4	the	the	DT
cord-014597-66vd2mdu	360	5	product	product	NN
cord-014597-66vd2mdu	360	6	quality	quality	NN
cord-014597-66vd2mdu	360	7	was	be	VBD
cord-014597-66vd2mdu	360	8	investigated	investigate	VBN
cord-014597-66vd2mdu	360	9	.	.	.
cord-014597-66vd2mdu	361	1	Compared	compare	VBN
cord-014597-66vd2mdu	361	2	to	to	IN
cord-014597-66vd2mdu	361	3	the	the	DT
cord-014597-66vd2mdu	361	4	legacy	legacy	NN
cord-014597-66vd2mdu	361	5	fedbatch	fedbatch	NN
cord-014597-66vd2mdu	361	6	process	process	NN
cord-014597-66vd2mdu	361	7	,	,	,
cord-014597-66vd2mdu	361	8	fed	fed	NNP
cord-014597-66vd2mdu	361	9	-	-	HYPH
cord-014597-66vd2mdu	361	10	batches	batch	NNS
cord-014597-66vd2mdu	361	11	that	that	WDT
cord-014597-66vd2mdu	361	12	were	be	VBD
cord-014597-66vd2mdu	361	13	conducted	conduct	VBN
cord-014597-66vd2mdu	361	14	with	with	IN
cord-014597-66vd2mdu	361	15	the	the	DT
cord-014597-66vd2mdu	361	16	newly	newly	RB
cord-014597-66vd2mdu	361	17	selected	select	VBN
cord-014597-66vd2mdu	361	18	basal	basal	NN
cord-014597-66vd2mdu	361	19	and	and	CC
cord-014597-66vd2mdu	361	20	feed	feed	NN
cord-014597-66vd2mdu	361	21	media	medium	NNS
cord-014597-66vd2mdu	361	22	altered	alter	VBD
cord-014597-66vd2mdu	361	23	the	the	DT
cord-014597-66vd2mdu	361	24	distribution	distribution	NN
cord-014597-66vd2mdu	361	25	of	of	IN
cord-014597-66vd2mdu	361	26	charge	charge	NN
cord-014597-66vd2mdu	361	27	and	and	CC
cord-014597-66vd2mdu	361	28	glycan	glycan	NN
cord-014597-66vd2mdu	361	29	variants	variant	NNS
cord-014597-66vd2mdu	361	30	.	.	.
cord-014597-66vd2mdu	362	1	The	the	DT
cord-014597-66vd2mdu	362	2	amount	amount	NN
cord-014597-66vd2mdu	362	3	of	of	IN
cord-014597-66vd2mdu	362	4	aggregated	aggregated	JJ
cord-014597-66vd2mdu	362	5	product	product	NN
cord-014597-66vd2mdu	362	6	was	be	VBD
cord-014597-66vd2mdu	362	7	not	not	RB
cord-014597-66vd2mdu	362	8	altered	alter	VBN
cord-014597-66vd2mdu	362	9	.	.	.
cord-014597-66vd2mdu	363	1	The	the	DT
cord-014597-66vd2mdu	363	2	established	established	JJ
cord-014597-66vd2mdu	363	3	methodology	methodology	NN
cord-014597-66vd2mdu	363	4	for	for	IN
cord-014597-66vd2mdu	363	5	fed	fed	JJ
cord-014597-66vd2mdu	363	6	-	-	HYPH
cord-014597-66vd2mdu	363	7	batch	batch	NN
cord-014597-66vd2mdu	363	8	development	development	NN
cord-014597-66vd2mdu	363	9	is	be	VBZ
cord-014597-66vd2mdu	363	10	a	a	DT
cord-014597-66vd2mdu	363	11	rapid	rapid	JJ
cord-014597-66vd2mdu	363	12	protocol	protocol	NN
cord-014597-66vd2mdu	363	13	to	to	TO
cord-014597-66vd2mdu	363	14	select	select	VB
cord-014597-66vd2mdu	363	15	well	well	RB
cord-014597-66vd2mdu	363	16	-	-	HYPH
cord-014597-66vd2mdu	363	17	performing	perform	VBG
cord-014597-66vd2mdu	363	18	feed	feed	NN
cord-014597-66vd2mdu	363	19	supplements	supplement	NNS
cord-014597-66vd2mdu	363	20	and	and	CC
cord-014597-66vd2mdu	363	21	optimize	optimize	VB
cord-014597-66vd2mdu	363	22	their	-PRON-	PRP$
cord-014597-66vd2mdu	363	23	ratio	ratio	NN
cord-014597-66vd2mdu	363	24	to	to	IN
cord-014597-66vd2mdu	363	25	the	the	DT
cord-014597-66vd2mdu	363	26	culture	culture	NN
cord-014597-66vd2mdu	363	27	requirements	requirement	NNS
cord-014597-66vd2mdu	363	28	.	.	.
cord-014597-66vd2mdu	364	1	In	in	IN
cord-014597-66vd2mdu	364	2	three	three	CD
cord-014597-66vd2mdu	364	3	steps	step	NNS
cord-014597-66vd2mdu	364	4	,	,	,
cord-014597-66vd2mdu	364	5	mAb	mAb	NNS
cord-014597-66vd2mdu	364	6	titers	titer	NNS
cord-014597-66vd2mdu	364	7	were	be	VBD
cord-014597-66vd2mdu	364	8	boosted	boost	VBN
cord-014597-66vd2mdu	364	9	2.5x	2.5x	CD
cord-014597-66vd2mdu	364	10	from	from	IN
cord-014597-66vd2mdu	364	11	1.9	1.9	CD
cord-014597-66vd2mdu	364	12	g	g	NN
cord-014597-66vd2mdu	364	13	/	/	SYM
cord-014597-66vd2mdu	364	14	L	l	NN
cord-014597-66vd2mdu	364	15	to	to	IN
cord-014597-66vd2mdu	364	16	4.9	4.9	CD
cord-014597-66vd2mdu	364	17	g	g	NN
cord-014597-66vd2mdu	364	18	/	/	SYM
cord-014597-66vd2mdu	364	19	L.	L.	NNP
cord-014597-66vd2mdu	364	20	Product	Product	NNP
cord-014597-66vd2mdu	364	21	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	364	22	and	and	CC
cord-014597-66vd2mdu	364	23	charge	charge	NN
cord-014597-66vd2mdu	364	24	variants	variant	NNS
cord-014597-66vd2mdu	364	25	could	could	MD
cord-014597-66vd2mdu	364	26	be	be	VB
cord-014597-66vd2mdu	364	27	influenced	influence	VBN
cord-014597-66vd2mdu	364	28	by	by	IN
cord-014597-66vd2mdu	364	29	the	the	DT
cord-014597-66vd2mdu	364	30	newly	newly	RB
cord-014597-66vd2mdu	364	31	selected	select	VBN
cord-014597-66vd2mdu	364	32	basal	basal	NN
cord-014597-66vd2mdu	364	33	and	and	CC
cord-014597-66vd2mdu	364	34	feed	feed	NN
cord-014597-66vd2mdu	364	35	media	medium	NNS
cord-014597-66vd2mdu	364	36	compared	compare	VBN
cord-014597-66vd2mdu	364	37	to	to	IN
cord-014597-66vd2mdu	364	38	a	a	DT
cord-014597-66vd2mdu	364	39	legacy	legacy	NN
cord-014597-66vd2mdu	364	40	fed	feed	VBN
cord-014597-66vd2mdu	364	41	-	-	HYPH
cord-014597-66vd2mdu	364	42	batch	batch	NN
cord-014597-66vd2mdu	364	43	process	process	NN
cord-014597-66vd2mdu	364	44	.	.	.
cord-014597-66vd2mdu	365	1	The	the	DT
cord-014597-66vd2mdu	365	2	amount	amount	NN
cord-014597-66vd2mdu	365	3	of	of	IN
cord-014597-66vd2mdu	365	4	aggregated	aggregated	JJ
cord-014597-66vd2mdu	365	5	product	product	NN
cord-014597-66vd2mdu	365	6	was	be	VBD
cord-014597-66vd2mdu	365	7	not	not	RB
cord-014597-66vd2mdu	365	8	altered	alter	VBN
cord-014597-66vd2mdu	365	9	.	.	.
cord-014597-66vd2mdu	366	1	The	the	DT
cord-014597-66vd2mdu	366	2	present	present	JJ
cord-014597-66vd2mdu	366	3	study	study	NN
cord-014597-66vd2mdu	366	4	investigates	investigate	VBZ
cord-014597-66vd2mdu	366	5	the	the	DT
cord-014597-66vd2mdu	366	6	beneficial	beneficial	JJ
cord-014597-66vd2mdu	366	7	effect	effect	NN
cord-014597-66vd2mdu	366	8	of	of	IN
cord-014597-66vd2mdu	366	9	spiking	spike	VBG
cord-014597-66vd2mdu	366	10	HyClone	HyClone	NNP
cord-014597-66vd2mdu	366	11	™	™	NN
cord-014597-66vd2mdu	366	12	ActiPro	ActiPro	NNP
cord-014597-66vd2mdu	366	13	™	™	NN
cord-014597-66vd2mdu	366	14	basal	basal	NN
cord-014597-66vd2mdu	366	15	medium	medium	NN
cord-014597-66vd2mdu	366	16	with	with	IN
cord-014597-66vd2mdu	366	17	HyClone	HyClone	NNP
cord-014597-66vd2mdu	366	18	Cell	Cell	NNP
cord-014597-66vd2mdu	366	19	Boost	Boost	NNP
cord-014597-66vd2mdu	366	20	™	™	CD
cord-014597-66vd2mdu	366	21	7a	7a	NN
cord-014597-66vd2mdu	366	22	and	and	CC
cord-014597-66vd2mdu	366	23	Cell	cell	NN
cord-014597-66vd2mdu	366	24	Boost	boost	NN
cord-014597-66vd2mdu	366	25	7b	7b	NN
cord-014597-66vd2mdu	366	26	feed	feed	VBP
cord-014597-66vd2mdu	366	27	supplements	supplement	NNS
cord-014597-66vd2mdu	366	28	on	on	IN
cord-014597-66vd2mdu	366	29	growth	growth	NN
cord-014597-66vd2mdu	366	30	and	and	CC
cord-014597-66vd2mdu	366	31	productivity	productivity	NN
cord-014597-66vd2mdu	366	32	of	of	IN
cord-014597-66vd2mdu	366	33	a	a	DT
cord-014597-66vd2mdu	366	34	recombinant	recombinant	JJ
cord-014597-66vd2mdu	366	35	CHO	CHO	NNP
cord-014597-66vd2mdu	366	36	cell	cell	NN
cord-014597-66vd2mdu	366	37	line	line	NN
cord-014597-66vd2mdu	366	38	.	.	.
cord-014597-66vd2mdu	367	1	To	to	TO
cord-014597-66vd2mdu	367	2	evaluate	evaluate	VB
cord-014597-66vd2mdu	367	3	the	the	DT
cord-014597-66vd2mdu	367	4	impact	impact	NN
cord-014597-66vd2mdu	367	5	of	of	IN
cord-014597-66vd2mdu	367	6	feed	feed	NN
cord-014597-66vd2mdu	367	7	-	-	HYPH
cord-014597-66vd2mdu	367	8	spiking	spiking	NN
cord-014597-66vd2mdu	367	9	compared	compare	VBN
cord-014597-66vd2mdu	367	10	with	with	IN
cord-014597-66vd2mdu	367	11	cultivation	cultivation	NN
cord-014597-66vd2mdu	367	12	in	in	IN
cord-014597-66vd2mdu	367	13	basal	basal	NN
cord-014597-66vd2mdu	367	14	medium	medium	NN
cord-014597-66vd2mdu	367	15	only	only	RB
cord-014597-66vd2mdu	367	16	,	,	,
cord-014597-66vd2mdu	367	17	the	the	DT
cord-014597-66vd2mdu	367	18	cell	cell	NN
cord-014597-66vd2mdu	367	19	line	line	NN
cord-014597-66vd2mdu	367	20	was	be	VBD
cord-014597-66vd2mdu	367	21	grown	grow	VBN
cord-014597-66vd2mdu	367	22	in	in	IN
cord-014597-66vd2mdu	367	23	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	367	24	under	under	IN
cord-014597-66vd2mdu	367	25	controlled	control	VBN
cord-014597-66vd2mdu	367	26	conditions	condition	NNS
cord-014597-66vd2mdu	367	27	to	to	TO
cord-014597-66vd2mdu	367	28	determine	determine	VB
cord-014597-66vd2mdu	367	29	cellspecific	cellspecific	JJ
cord-014597-66vd2mdu	367	30	metabolic	metabolic	JJ
cord-014597-66vd2mdu	367	31	rates	rate	NNS
cord-014597-66vd2mdu	367	32	,	,	,
cord-014597-66vd2mdu	367	33	nutrient	nutrient	NN
cord-014597-66vd2mdu	367	34	consumption	consumption	NN
cord-014597-66vd2mdu	367	35	,	,	,
cord-014597-66vd2mdu	367	36	and	and	CC
cord-014597-66vd2mdu	367	37	byproduct	byproduct	NN
cord-014597-66vd2mdu	367	38	accumulation	accumulation	NN
cord-014597-66vd2mdu	367	39	over	over	IN
cord-014597-66vd2mdu	367	40	the	the	DT
cord-014597-66vd2mdu	367	41	process	process	NN
cord-014597-66vd2mdu	367	42	time	time	NN
cord-014597-66vd2mdu	367	43	.	.	.
cord-014597-66vd2mdu	368	1	Transcriptome	Transcriptome	NNP
cord-014597-66vd2mdu	368	2	analysis	analysis	NN
cord-014597-66vd2mdu	368	3	of	of	IN
cord-014597-66vd2mdu	368	4	the	the	DT
cord-014597-66vd2mdu	368	5	cultivated	cultivate	VBN
cord-014597-66vd2mdu	368	6	cells	cell	NNS
cord-014597-66vd2mdu	368	7	,	,	,
cord-014597-66vd2mdu	368	8	using	use	VBG
cord-014597-66vd2mdu	368	9	microarrays	microarray	NNS
cord-014597-66vd2mdu	368	10	on	on	IN
cord-014597-66vd2mdu	368	11	four	four	CD
cord-014597-66vd2mdu	368	12	consecutive	consecutive	JJ
cord-014597-66vd2mdu	368	13	days	day	NNS
cord-014597-66vd2mdu	368	14	to	to	TO
cord-014597-66vd2mdu	368	15	investigate	investigate	VB
cord-014597-66vd2mdu	368	16	differential	differential	JJ
cord-014597-66vd2mdu	368	17	gene	gene	NN
cord-014597-66vd2mdu	368	18	expression	expression	NN
cord-014597-66vd2mdu	368	19	,	,	,
cord-014597-66vd2mdu	368	20	revealed	reveal	VBD
cord-014597-66vd2mdu	368	21	the	the	DT
cord-014597-66vd2mdu	368	22	beneficial	beneficial	JJ
cord-014597-66vd2mdu	368	23	effect	effect	NN
cord-014597-66vd2mdu	368	24	of	of	IN
cord-014597-66vd2mdu	368	25	feed	feed	NN
cord-014597-66vd2mdu	368	26	-	-	HYPH
cord-014597-66vd2mdu	368	27	spiking	spiking	NN
cord-014597-66vd2mdu	368	28	compared	compare	VBN
cord-014597-66vd2mdu	368	29	with	with	IN
cord-014597-66vd2mdu	368	30	cells	cell	NNS
cord-014597-66vd2mdu	368	31	grown	grow	VBN
cord-014597-66vd2mdu	368	32	in	in	IN
cord-014597-66vd2mdu	368	33	basal	basal	NN
cord-014597-66vd2mdu	368	34	medium	medium	NN
cord-014597-66vd2mdu	368	35	.	.	.
cord-014597-66vd2mdu	369	1	Model	model	NN
cord-014597-66vd2mdu	369	2	cell	cell	NN
cord-014597-66vd2mdu	369	3	line	line	NN
cord-014597-66vd2mdu	369	4	was	be	VBD
cord-014597-66vd2mdu	369	5	a	a	DT
cord-014597-66vd2mdu	369	6	mAb	mab	NN
cord-014597-66vd2mdu	369	7	-	-	HYPH
cord-014597-66vd2mdu	369	8	expressing	express	VBG
cord-014597-66vd2mdu	369	9	CHO	CHO	NNP
cord-014597-66vd2mdu	369	10	DG44	DG44	NNP
cord-014597-66vd2mdu	369	11	(	(	-LRB-
cord-014597-66vd2mdu	369	12	licensed	license	VBN
cord-014597-66vd2mdu	369	13	from	from	IN
cord-014597-66vd2mdu	369	14	Cellca	Cellca	NNP
cord-014597-66vd2mdu	369	15	GmbH	GmbH	NNP
cord-014597-66vd2mdu	369	16	)	)	-RRB-
cord-014597-66vd2mdu	369	17	cultivated	cultivate	VBD
cord-014597-66vd2mdu	369	18	either	either	CC
cord-014597-66vd2mdu	369	19	in	in	IN
cord-014597-66vd2mdu	369	20	ActiPro	ActiPro	NNP
cord-014597-66vd2mdu	369	21	basal	basal	NN
cord-014597-66vd2mdu	369	22	medium	medium	NN
cord-014597-66vd2mdu	369	23	only	only	RB
cord-014597-66vd2mdu	369	24	(	(	-LRB-
cord-014597-66vd2mdu	369	25	GE	GE	NNP
cord-014597-66vd2mdu	369	26	Healthcare	Healthcare	NNP
cord-014597-66vd2mdu	369	27	)	)	-RRB-
cord-014597-66vd2mdu	369	28	or	or	CC
cord-014597-66vd2mdu	369	29	in	in	IN
cord-014597-66vd2mdu	369	30	ActiPro	ActiPro	NNP
cord-014597-66vd2mdu	369	31	basal	basal	NN
cord-014597-66vd2mdu	369	32	medium	medium	NN
cord-014597-66vd2mdu	369	33	feed	feed	NN
cord-014597-66vd2mdu	369	34	-	-	HYPH
cord-014597-66vd2mdu	369	35	spiked	spike	VBN
cord-014597-66vd2mdu	369	36	with	with	IN
cord-014597-66vd2mdu	369	37	additional	additional	JJ
cord-014597-66vd2mdu	369	38	supplementation	supplementation	NN
cord-014597-66vd2mdu	369	39	with	with	IN
cord-014597-66vd2mdu	369	40	7	7	CD
cord-014597-66vd2mdu	369	41	%	%	NN
cord-014597-66vd2mdu	369	42	Cell	cell	NN
cord-014597-66vd2mdu	369	43	Boost	Boost	NNP
cord-014597-66vd2mdu	369	44	7a	7a	NN
cord-014597-66vd2mdu	369	45	and	and	CC
cord-014597-66vd2mdu	369	46	0.7	0.7	CD
cord-014597-66vd2mdu	369	47	%	%	NN
cord-014597-66vd2mdu	369	48	Cell	cell	NN
cord-014597-66vd2mdu	369	49	Boost	boost	NN
cord-014597-66vd2mdu	369	50	7b	7b	NN
cord-014597-66vd2mdu	369	51	(	(	-LRB-
cord-014597-66vd2mdu	369	52	GE	GE	NNP
cord-014597-66vd2mdu	369	53	Healthcare	Healthcare	NNP
cord-014597-66vd2mdu	369	54	)	)	-RRB-
cord-014597-66vd2mdu	369	55	.	.	.
cord-014597-66vd2mdu	370	1	Both	both	DT
cord-014597-66vd2mdu	370	2	cultures	culture	NNS
cord-014597-66vd2mdu	370	3	were	be	VBD
cord-014597-66vd2mdu	370	4	grown	grow	VBN
cord-014597-66vd2mdu	370	5	in	in	IN
cord-014597-66vd2mdu	370	6	batch	batch	NN
cord-014597-66vd2mdu	370	7	mode	mode	NN
cord-014597-66vd2mdu	370	8	using	use	VBG
cord-014597-66vd2mdu	370	9	DASGIP	DASGIP	NNP
cord-014597-66vd2mdu	370	10	™	™	HYPH
cord-014597-66vd2mdu	370	11	CellFerm	CellFerm	NNP
cord-014597-66vd2mdu	370	12	-	-	HYPH
cord-014597-66vd2mdu	370	13	Pro	Pro	NNP
cord-014597-66vd2mdu	370	14	™	™	NN
cord-014597-66vd2mdu	370	15	stirred	stir	VBN
cord-014597-66vd2mdu	370	16	-	-	HYPH
cord-014597-66vd2mdu	370	17	tank	tank	NN
cord-014597-66vd2mdu	370	18	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	370	19	(	(	-LRB-
cord-014597-66vd2mdu	370	20	Eppendorf	Eppendorf	NNP
cord-014597-66vd2mdu	370	21	)	)	-RRB-
cord-014597-66vd2mdu	370	22	.	.	.
cord-014597-66vd2mdu	371	1	The	the	DT
cord-014597-66vd2mdu	371	2	beneficial	beneficial	JJ
cord-014597-66vd2mdu	371	3	effect	effect	NN
cord-014597-66vd2mdu	371	4	of	of	IN
cord-014597-66vd2mdu	371	5	feed	feed	NN
cord-014597-66vd2mdu	371	6	-	-	HYPH
cord-014597-66vd2mdu	371	7	spiking	spiking	NN
cord-014597-66vd2mdu	371	8	was	be	VBD
cord-014597-66vd2mdu	371	9	analysed	analyse	VBN
cord-014597-66vd2mdu	371	10	by	by	IN
cord-014597-66vd2mdu	371	11	transcriptome	transcriptome	NN
cord-014597-66vd2mdu	371	12	analysis	analysis	NN
cord-014597-66vd2mdu	371	13	using	use	VBG
cord-014597-66vd2mdu	371	14	microarray	microarray	NN
cord-014597-66vd2mdu	371	15	technology	technology	NN
cord-014597-66vd2mdu	371	16	(	(	-LRB-
cord-014597-66vd2mdu	371	17	8×60	8×60	CD
cord-014597-66vd2mdu	371	18	k	k	NN
cord-014597-66vd2mdu	371	19	design	design	NN
cord-014597-66vd2mdu	371	20	,	,	,
cord-014597-66vd2mdu	371	21	Agilent	Agilent	NNP
cord-014597-66vd2mdu	371	22	)	)	-RRB-
cord-014597-66vd2mdu	371	23	.	.	.
cord-014597-66vd2mdu	372	1	Both	both	DT
cord-014597-66vd2mdu	372	2	basal	basal	NN
cord-014597-66vd2mdu	372	3	and	and	CC
cord-014597-66vd2mdu	372	4	feed	feed	NN
cord-014597-66vd2mdu	372	5	-	-	HYPH
cord-014597-66vd2mdu	372	6	spiked	spike	VBN
cord-014597-66vd2mdu	372	7	processes	process	NNS
cord-014597-66vd2mdu	372	8	lasted	last	VBD
cord-014597-66vd2mdu	372	9	for	for	IN
cord-014597-66vd2mdu	372	10	seven	seven	CD
cord-014597-66vd2mdu	372	11	days	day	NNS
cord-014597-66vd2mdu	372	12	with	with	IN
cord-014597-66vd2mdu	372	13	viabilities	viability	NNS
cord-014597-66vd2mdu	372	14	above	above	IN
cord-014597-66vd2mdu	372	15	95	95	CD
cord-014597-66vd2mdu	372	16	%	%	NN
cord-014597-66vd2mdu	372	17	until	until	IN
cord-014597-66vd2mdu	372	18	Day	day	NN
cord-014597-66vd2mdu	372	19	6	6	CD
cord-014597-66vd2mdu	372	20	.	.	.
cord-014597-66vd2mdu	373	1	On	on	IN
cord-014597-66vd2mdu	373	2	day	day	NN
cord-014597-66vd2mdu	373	3	seven	seven	CD
cord-014597-66vd2mdu	373	4	,	,	,
cord-014597-66vd2mdu	373	5	a	a	DT
cord-014597-66vd2mdu	373	6	sharp	sharp	JJ
cord-014597-66vd2mdu	373	7	decline	decline	NN
cord-014597-66vd2mdu	373	8	in	in	IN
cord-014597-66vd2mdu	373	9	viability	viability	NN
cord-014597-66vd2mdu	373	10	indicated	indicate	VBD
cord-014597-66vd2mdu	373	11	the	the	DT
cord-014597-66vd2mdu	373	12	end	end	NN
cord-014597-66vd2mdu	373	13	of	of	IN
cord-014597-66vd2mdu	373	14	the	the	DT
cord-014597-66vd2mdu	373	15	batch	batch	NN
cord-014597-66vd2mdu	373	16	process	process	NN
cord-014597-66vd2mdu	373	17	(	(	-LRB-
cord-014597-66vd2mdu	373	18	Fig	Fig	NNP
cord-014597-66vd2mdu	373	19	.	.	.
cord-014597-66vd2mdu	373	20	1a	1a	CD
cord-014597-66vd2mdu	373	21	)	)	-RRB-
cord-014597-66vd2mdu	373	22	.	.	.
cord-014597-66vd2mdu	374	1	In	in	IN
cord-014597-66vd2mdu	374	2	feed	feed	NN
cord-014597-66vd2mdu	374	3	-	-	HYPH
cord-014597-66vd2mdu	374	4	spiked	spike	VBN
cord-014597-66vd2mdu	374	5	medium	medium	NN
cord-014597-66vd2mdu	374	6	,	,	,
cord-014597-66vd2mdu	374	7	cells	cell	NNS
cord-014597-66vd2mdu	374	8	initially	initially	RB
cord-014597-66vd2mdu	374	9	grew	grow	VBD
cord-014597-66vd2mdu	374	10	slower	slow	RBR
cord-014597-66vd2mdu	374	11	but	but	CC
cord-014597-66vd2mdu	374	12	reached	reach	VBD
cord-014597-66vd2mdu	374	13	almost	almost	RB
cord-014597-66vd2mdu	374	14	twice	twice	PDT
cord-014597-66vd2mdu	374	15	as	as	RB
cord-014597-66vd2mdu	374	16	high	high	JJ
cord-014597-66vd2mdu	374	17	peak	peak	NN
cord-014597-66vd2mdu	374	18	cell	cell	NN
cord-014597-66vd2mdu	374	19	concentrations	concentration	NNS
cord-014597-66vd2mdu	374	20	(	(	-LRB-
cord-014597-66vd2mdu	374	21	17.6	17.6	CD
cord-014597-66vd2mdu	374	22	×	×	NN
cord-014597-66vd2mdu	374	23	10	10	CD
cord-014597-66vd2mdu	374	24	6	6	CD
cord-014597-66vd2mdu	374	25	c	c	NN
cord-014597-66vd2mdu	374	26	/	/	SYM
cord-014597-66vd2mdu	374	27	mL	ml	NN
cord-014597-66vd2mdu	374	28	)	)	-RRB-
cord-014597-66vd2mdu	374	29	than	than	IN
cord-014597-66vd2mdu	374	30	in	in	IN
cord-014597-66vd2mdu	374	31	basal	basal	NN
cord-014597-66vd2mdu	374	32	medium	medium	NN
cord-014597-66vd2mdu	374	33	only	only	RB
cord-014597-66vd2mdu	374	34	(	(	-LRB-
cord-014597-66vd2mdu	374	35	9.79	9.79	CD
cord-014597-66vd2mdu	374	36	×	×	NN
cord-014597-66vd2mdu	374	37	10	10	CD
cord-014597-66vd2mdu	374	38	6	6	CD
cord-014597-66vd2mdu	374	39	c	c	NN
cord-014597-66vd2mdu	374	40	/	/	SYM
cord-014597-66vd2mdu	374	41	mL	ml	NN
cord-014597-66vd2mdu	374	42	)	)	-RRB-
cord-014597-66vd2mdu	374	43	.	.	.
cord-014597-66vd2mdu	375	1	Remarkably	remarkably	RB
cord-014597-66vd2mdu	375	2	,	,	,
cord-014597-66vd2mdu	375	3	the	the	DT
cord-014597-66vd2mdu	375	4	integral	integral	NN
cord-014597-66vd2mdu	375	5	of	of	IN
cord-014597-66vd2mdu	375	6	the	the	DT
cord-014597-66vd2mdu	375	7	viable	viable	JJ
cord-014597-66vd2mdu	375	8	cell	cell	NN
cord-014597-66vd2mdu	375	9	concentration	concentration	NN
cord-014597-66vd2mdu	375	10	over	over	IN
cord-014597-66vd2mdu	375	11	the	the	DT
cord-014597-66vd2mdu	375	12	total	total	JJ
cord-014597-66vd2mdu	375	13	process	process	NN
cord-014597-66vd2mdu	375	14	time	time	NN
cord-014597-66vd2mdu	375	15	(	(	-LRB-
cord-014597-66vd2mdu	375	16	viable	viable	JJ
cord-014597-66vd2mdu	375	17	cumulative	cumulative	JJ
cord-014597-66vd2mdu	375	18	cell	cell	NN
cord-014597-66vd2mdu	375	19	days	day	NNS
cord-014597-66vd2mdu	375	20	[	[	-LRB-
cord-014597-66vd2mdu	375	21	VCCD	VCCD	NNP
cord-014597-66vd2mdu	375	22	]	]	-RRB-
cord-014597-66vd2mdu	375	23	)	)	-RRB-
cord-014597-66vd2mdu	375	24	was	be	VBD
cord-014597-66vd2mdu	375	25	similar	similar	JJ
cord-014597-66vd2mdu	375	26	between	between	IN
cord-014597-66vd2mdu	375	27	both	both	DT
cord-014597-66vd2mdu	375	28	process	process	NN
cord-014597-66vd2mdu	375	29	strategies	strategy	NNS
cord-014597-66vd2mdu	375	30	(	(	-LRB-
cord-014597-66vd2mdu	375	31	Fig	Fig	NNP
cord-014597-66vd2mdu	375	32	.	.	.
cord-014597-66vd2mdu	375	33	1c	1c	CD
cord-014597-66vd2mdu	375	34	)	)	-RRB-
cord-014597-66vd2mdu	375	35	.	.	.
cord-014597-66vd2mdu	376	1	While	while	IN
cord-014597-66vd2mdu	376	2	mAb	mab	NN
cord-014597-66vd2mdu	376	3	production	production	NN
cord-014597-66vd2mdu	376	4	plateaued	plateaue	VBD
cord-014597-66vd2mdu	376	5	after	after	IN
cord-014597-66vd2mdu	376	6	Day	day	NN
cord-014597-66vd2mdu	376	7	4	4	CD
cord-014597-66vd2mdu	376	8	in	in	IN
cord-014597-66vd2mdu	376	9	basal	basal	NN
cord-014597-66vd2mdu	376	10	medium	medium	NN
cord-014597-66vd2mdu	376	11	only	only	RB
cord-014597-66vd2mdu	376	12	(	(	-LRB-
cord-014597-66vd2mdu	376	13	final	final	JJ
cord-014597-66vd2mdu	376	14	titer	titer	NN
cord-014597-66vd2mdu	376	15	0.8	0.8	CD
cord-014597-66vd2mdu	376	16	g	g	NN
cord-014597-66vd2mdu	376	17	/	/	SYM
cord-014597-66vd2mdu	376	18	L	L	NNP
cord-014597-66vd2mdu	376	19	)	)	-RRB-
cord-014597-66vd2mdu	376	20	,	,	,
cord-014597-66vd2mdu	376	21	a	a	DT
cord-014597-66vd2mdu	376	22	continuous	continuous	JJ
cord-014597-66vd2mdu	376	23	increase	increase	NN
cord-014597-66vd2mdu	376	24	to	to	IN
cord-014597-66vd2mdu	376	25	three	three	CD
cord-014597-66vd2mdu	376	26	-	-	HYPH
cord-014597-66vd2mdu	376	27	fold	fold	VB
cord-014597-66vd2mdu	376	28	higher	high	JJR
cord-014597-66vd2mdu	376	29	final	final	JJ
cord-014597-66vd2mdu	376	30	titers	titer	NNS
cord-014597-66vd2mdu	376	31	(	(	-LRB-
cord-014597-66vd2mdu	376	32	2.4	2.4	CD
cord-014597-66vd2mdu	376	33	g	g	NN
cord-014597-66vd2mdu	376	34	/	/	SYM
cord-014597-66vd2mdu	376	35	L	L	NNP
cord-014597-66vd2mdu	376	36	)	)	-RRB-
cord-014597-66vd2mdu	376	37	was	be	VBD
cord-014597-66vd2mdu	376	38	observed	observe	VBN
cord-014597-66vd2mdu	376	39	in	in	IN
cord-014597-66vd2mdu	376	40	feed	feed	NN
cord-014597-66vd2mdu	376	41	-	-	HYPH
cord-014597-66vd2mdu	376	42	spiked	spike	VBN
cord-014597-66vd2mdu	376	43	medium	medium	NN
cord-014597-66vd2mdu	376	44	(	(	-LRB-
cord-014597-66vd2mdu	376	45	Fig	Fig	NNP
cord-014597-66vd2mdu	376	46	.	.	.
cord-014597-66vd2mdu	376	47	1b	1b	CD
cord-014597-66vd2mdu	376	48	)	)	-RRB-
cord-014597-66vd2mdu	376	49	.	.	.
cord-014597-66vd2mdu	377	1	The	the	DT
cord-014597-66vd2mdu	377	2	higher	high	JJR
cord-014597-66vd2mdu	377	3	titers	titer	NNS
cord-014597-66vd2mdu	377	4	could	could	MD
cord-014597-66vd2mdu	377	5	be	be	VB
cord-014597-66vd2mdu	377	6	attributed	attribute	VBN
cord-014597-66vd2mdu	377	7	to	to	IN
cord-014597-66vd2mdu	377	8	generally	generally	RB
cord-014597-66vd2mdu	377	9	higher	high	JJR
cord-014597-66vd2mdu	377	10	cell	cell	NN
cord-014597-66vd2mdu	377	11	-	-	HYPH
cord-014597-66vd2mdu	377	12	specific	specific	JJ
cord-014597-66vd2mdu	377	13	productivities	productivity	NNS
cord-014597-66vd2mdu	377	14	(	(	-LRB-
cord-014597-66vd2mdu	377	15	qP	qP	NNP
cord-014597-66vd2mdu	377	16	)	)	-RRB-
cord-014597-66vd2mdu	377	17	,	,	,
cord-014597-66vd2mdu	377	18	which	which	WDT
cord-014597-66vd2mdu	377	19	remained	remain	VBD
cord-014597-66vd2mdu	377	20	rather	rather	RB
cord-014597-66vd2mdu	377	21	constant	constant	JJ
cord-014597-66vd2mdu	377	22	(	(	-LRB-
cord-014597-66vd2mdu	377	23	~70	~70	NFP
cord-014597-66vd2mdu	377	24	pg	pg	NN
cord-014597-66vd2mdu	377	25	/	/	SYM
cord-014597-66vd2mdu	377	26	cell	cell	NN
cord-014597-66vd2mdu	377	27	/	/	SYM
cord-014597-66vd2mdu	377	28	day	day	NN
cord-014597-66vd2mdu	377	29	)	)	-RRB-
cord-014597-66vd2mdu	377	30	in	in	IN
cord-014597-66vd2mdu	377	31	feedspiked	feedspiked	JJ
cord-014597-66vd2mdu	377	32	cultures	culture	NNS
cord-014597-66vd2mdu	377	33	.	.	.
cord-014597-66vd2mdu	378	1	In	in	IN
cord-014597-66vd2mdu	378	2	basal	basal	NN
cord-014597-66vd2mdu	378	3	medium	medium	NN
cord-014597-66vd2mdu	378	4	,	,	,
cord-014597-66vd2mdu	378	5	the	the	DT
cord-014597-66vd2mdu	378	6	qP	qP	NNP
cord-014597-66vd2mdu	378	7	continuously	continuously	RB
cord-014597-66vd2mdu	378	8	dropped	drop	VBD
cord-014597-66vd2mdu	378	9	by	by	IN
cord-014597-66vd2mdu	378	10	20	20	CD
cord-014597-66vd2mdu	378	11	%	%	NN
cord-014597-66vd2mdu	378	12	(	(	-LRB-
cord-014597-66vd2mdu	378	13	Day	day	NN
cord-014597-66vd2mdu	378	14	0	0	CD
cord-014597-66vd2mdu	378	15	to	to	IN
cord-014597-66vd2mdu	378	16	3	3	CD
cord-014597-66vd2mdu	378	17	)	)	-RRB-
cord-014597-66vd2mdu	378	18	,	,	,
cord-014597-66vd2mdu	378	19	50	50	CD
cord-014597-66vd2mdu	378	20	%	%	NN
cord-014597-66vd2mdu	378	21	(	(	-LRB-
cord-014597-66vd2mdu	378	22	Day	Day	NNP
cord-014597-66vd2mdu	378	23	4	4	CD
cord-014597-66vd2mdu	378	24	)	)	-RRB-
cord-014597-66vd2mdu	378	25	,	,	,
cord-014597-66vd2mdu	378	26	and	and	CC
cord-014597-66vd2mdu	378	27	>	>	XX
cord-014597-66vd2mdu	378	28	90	90	CD
cord-014597-66vd2mdu	378	29	%	%	NN
cord-014597-66vd2mdu	378	30	(	(	-LRB-
cord-014597-66vd2mdu	378	31	Day	day	NN
cord-014597-66vd2mdu	378	32	5	5	CD
cord-014597-66vd2mdu	378	33	to	to	IN
cord-014597-66vd2mdu	378	34	7	7	CD
cord-014597-66vd2mdu	378	35	)	)	-RRB-
cord-014597-66vd2mdu	378	36	from	from	IN
cord-014597-66vd2mdu	378	37	70	70	CD
cord-014597-66vd2mdu	378	38	to	to	TO
cord-014597-66vd2mdu	378	39	10	10	CD
cord-014597-66vd2mdu	378	40	pg	pg	NN
cord-014597-66vd2mdu	378	41	/	/	SYM
cord-014597-66vd2mdu	378	42	cell	cell	NN
cord-014597-66vd2mdu	378	43	/	/	SYM
cord-014597-66vd2mdu	378	44	day	day	NN
cord-014597-66vd2mdu	378	45	in	in	IN
cord-014597-66vd2mdu	378	46	basal	basal	NN
cord-014597-66vd2mdu	378	47	medium	medium	NN
cord-014597-66vd2mdu	378	48	cultures	culture	NNS
cord-014597-66vd2mdu	378	49	.	.	.
cord-014597-66vd2mdu	379	1	In	in	IN
cord-014597-66vd2mdu	379	2	average	average	JJ
cord-014597-66vd2mdu	379	3	,	,	,
cord-014597-66vd2mdu	379	4	the	the	DT
cord-014597-66vd2mdu	379	5	qP	qP	NNP
cord-014597-66vd2mdu	379	6	was	be	VBD
cord-014597-66vd2mdu	379	7	70	70	CD
cord-014597-66vd2mdu	379	8	%	%	NN
cord-014597-66vd2mdu	379	9	higher	high	JJR
cord-014597-66vd2mdu	379	10	in	in	IN
cord-014597-66vd2mdu	379	11	feed	feed	NN
cord-014597-66vd2mdu	379	12	-	-	HYPH
cord-014597-66vd2mdu	379	13	spiked	spike	VBN
cord-014597-66vd2mdu	379	14	cultures	culture	NNS
cord-014597-66vd2mdu	379	15	(	(	-LRB-
cord-014597-66vd2mdu	379	16	Fig	Fig	NNP
cord-014597-66vd2mdu	379	17	.	.	.
cord-014597-66vd2mdu	379	18	1d	1d	CD
cord-014597-66vd2mdu	379	19	)	)	-RRB-
cord-014597-66vd2mdu	379	20	.	.	.
cord-014597-66vd2mdu	380	1	Transcriptome	Transcriptome	NNP
cord-014597-66vd2mdu	380	2	analysis	analysis	NN
cord-014597-66vd2mdu	380	3	of	of	IN
cord-014597-66vd2mdu	380	4	differentially	differentially	RB
cord-014597-66vd2mdu	380	5	expressed	express	VBN
cord-014597-66vd2mdu	380	6	genes	gene	NNS
cord-014597-66vd2mdu	380	7	between	between	IN
cord-014597-66vd2mdu	380	8	cells	cell	NNS
cord-014597-66vd2mdu	380	9	grown	grow	VBN
cord-014597-66vd2mdu	380	10	in	in	IN
cord-014597-66vd2mdu	380	11	basal	basal	NN
cord-014597-66vd2mdu	380	12	medium	medium	NN
cord-014597-66vd2mdu	380	13	or	or	CC
cord-014597-66vd2mdu	380	14	feed	feed	NN
cord-014597-66vd2mdu	380	15	-	-	HYPH
cord-014597-66vd2mdu	380	16	spiked	spike	VBN
cord-014597-66vd2mdu	380	17	medium	medium	NN
cord-014597-66vd2mdu	380	18	were	be	VBD
cord-014597-66vd2mdu	380	19	used	use	VBN
cord-014597-66vd2mdu	380	20	to	to	TO
cord-014597-66vd2mdu	380	21	identify	identify	VB
cord-014597-66vd2mdu	380	22	relevant	relevant	JJ
cord-014597-66vd2mdu	380	23	GO	GO	NNP
cord-014597-66vd2mdu	380	24	terms	term	NNS
cord-014597-66vd2mdu	380	25	that	that	WDT
cord-014597-66vd2mdu	380	26	indicated	indicate	VBD
cord-014597-66vd2mdu	380	27	a	a	DT
cord-014597-66vd2mdu	380	28	more	more	RBR
cord-014597-66vd2mdu	380	29	active	active	JJ
cord-014597-66vd2mdu	380	30	proliferative	proliferative	JJ
cord-014597-66vd2mdu	380	31	state	state	NN
cord-014597-66vd2mdu	380	32	for	for	IN
cord-014597-66vd2mdu	380	33	feed	feed	NN
cord-014597-66vd2mdu	380	34	-	-	HYPH
cord-014597-66vd2mdu	380	35	spiked	spike	VBN
cord-014597-66vd2mdu	380	36	cultures	culture	NNS
cord-014597-66vd2mdu	380	37	(	(	-LRB-
cord-014597-66vd2mdu	380	38	data	datum	NNS
cord-014597-66vd2mdu	380	39	not	not	RB
cord-014597-66vd2mdu	380	40	shown	show	VBN
cord-014597-66vd2mdu	380	41	)	)	-RRB-
cord-014597-66vd2mdu	380	42	.	.	.
cord-014597-66vd2mdu	381	1	The	the	DT
cord-014597-66vd2mdu	381	2	top	top	JJ
cord-014597-66vd2mdu	381	3	GO	GO	NNP
cord-014597-66vd2mdu	381	4	terms	term	NNS
cord-014597-66vd2mdu	381	5	significantly	significantly	RB
cord-014597-66vd2mdu	381	6	related	relate	VBN
cord-014597-66vd2mdu	381	7	to	to	IN
cord-014597-66vd2mdu	381	8	cell	cell	NN
cord-014597-66vd2mdu	381	9	cycle	cycle	NN
cord-014597-66vd2mdu	381	10	and	and	CC
cord-014597-66vd2mdu	381	11	primary	primary	JJ
cord-014597-66vd2mdu	381	12	metabolism	metabolism	NN
cord-014597-66vd2mdu	381	13	,	,	,
cord-014597-66vd2mdu	381	14	cellular	cellular	JJ
cord-014597-66vd2mdu	381	15	division	division	NN
cord-014597-66vd2mdu	381	16	,	,	,
cord-014597-66vd2mdu	381	17	as	as	RB
cord-014597-66vd2mdu	381	18	well	well	RB
cord-014597-66vd2mdu	381	19	as	as	IN
cord-014597-66vd2mdu	381	20	nucleobase	nucleobase	NNP
cord-014597-66vd2mdu	381	21	formation	formation	NN
cord-014597-66vd2mdu	381	22	or	or	CC
cord-014597-66vd2mdu	381	23	regulation	regulation	NN
cord-014597-66vd2mdu	381	24	.	.	.
cord-014597-66vd2mdu	382	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	382	2	,	,	,
cord-014597-66vd2mdu	382	3	GSEA	GSEA	NNP
cord-014597-66vd2mdu	382	4	revealed	reveal	VBD
cord-014597-66vd2mdu	382	5	several	several	JJ
cord-014597-66vd2mdu	382	6	significantly	significantly	RB
cord-014597-66vd2mdu	382	7	enriched	enrich	VBN
cord-014597-66vd2mdu	382	8	set	set	NN
cord-014597-66vd2mdu	382	9	of	of	IN
cord-014597-66vd2mdu	382	10	genes	gene	NNS
cord-014597-66vd2mdu	382	11	related	relate	VBN
cord-014597-66vd2mdu	382	12	to	to	IN
cord-014597-66vd2mdu	382	13	gene	gene	NN
cord-014597-66vd2mdu	382	14	transcription	transcription	NN
cord-014597-66vd2mdu	382	15	,	,	,
cord-014597-66vd2mdu	382	16	DNA	dna	NN
cord-014597-66vd2mdu	382	17	replication	replication	NN
cord-014597-66vd2mdu	382	18	and	and	CC
cord-014597-66vd2mdu	382	19	repair	repair	NN
cord-014597-66vd2mdu	382	20	,	,	,
cord-014597-66vd2mdu	382	21	cell	cell	NN
cord-014597-66vd2mdu	382	22	growth	growth	NN
cord-014597-66vd2mdu	382	23	and	and	CC
cord-014597-66vd2mdu	382	24	proliferation	proliferation	NN
cord-014597-66vd2mdu	382	25	,	,	,
cord-014597-66vd2mdu	382	26	as	as	RB
cord-014597-66vd2mdu	382	27	well	well	RB
cord-014597-66vd2mdu	382	28	as	as	IN
cord-014597-66vd2mdu	382	29	inhibition	inhibition	NN
cord-014597-66vd2mdu	382	30	of	of	IN
cord-014597-66vd2mdu	382	31	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	382	32	in	in	IN
cord-014597-66vd2mdu	382	33	feed	feed	NN
cord-014597-66vd2mdu	382	34	-	-	HYPH
cord-014597-66vd2mdu	382	35	spiked	spike	VBN
cord-014597-66vd2mdu	382	36	cultures	culture	NNS
cord-014597-66vd2mdu	382	37	.	.	.
cord-014597-66vd2mdu	383	1	Thus	thus	RB
cord-014597-66vd2mdu	383	2	,	,	,
cord-014597-66vd2mdu	383	3	feed	feed	NN
cord-014597-66vd2mdu	383	4	-	-	HYPH
cord-014597-66vd2mdu	383	5	spiking	spiking	NN
cord-014597-66vd2mdu	383	6	increased	increase	VBD
cord-014597-66vd2mdu	383	7	the	the	DT
cord-014597-66vd2mdu	383	8	proliferative	proliferative	JJ
cord-014597-66vd2mdu	383	9	activity	activity	NN
cord-014597-66vd2mdu	383	10	of	of	IN
cord-014597-66vd2mdu	383	11	cultivated	cultivate	VBN
cord-014597-66vd2mdu	383	12	cells	cell	NNS
cord-014597-66vd2mdu	383	13	.	.	.
cord-014597-66vd2mdu	384	1	Several	several	JJ
cord-014597-66vd2mdu	384	2	of	of	IN
cord-014597-66vd2mdu	384	3	the	the	DT
cord-014597-66vd2mdu	384	4	identified	identify	VBN
cord-014597-66vd2mdu	384	5	genes	gene	NNS
cord-014597-66vd2mdu	384	6	appear	appear	VBP
cord-014597-66vd2mdu	384	7	as	as	IN
cord-014597-66vd2mdu	384	8	promising	promising	JJ
cord-014597-66vd2mdu	384	9	targets	target	NNS
cord-014597-66vd2mdu	384	10	for	for	IN
cord-014597-66vd2mdu	384	11	cell	cell	NN
cord-014597-66vd2mdu	384	12	line	line	NN
cord-014597-66vd2mdu	384	13	engineering	engineering	NN
cord-014597-66vd2mdu	384	14	,	,	,
cord-014597-66vd2mdu	384	15	but	but	CC
cord-014597-66vd2mdu	384	16	have	have	VBP
cord-014597-66vd2mdu	384	17	not	not	RB
cord-014597-66vd2mdu	384	18	yet	yet	RB
cord-014597-66vd2mdu	384	19	been	be	VBN
cord-014597-66vd2mdu	384	20	described	describe	VBN
cord-014597-66vd2mdu	384	21	in	in	IN
cord-014597-66vd2mdu	384	22	relation	relation	NN
cord-014597-66vd2mdu	384	23	to	to	IN
cord-014597-66vd2mdu	384	24	high	high	RB
cord-014597-66vd2mdu	384	25	-	-	HYPH
cord-014597-66vd2mdu	384	26	producing	produce	VBG
cord-014597-66vd2mdu	384	27	recombinant	recombinant	JJ
cord-014597-66vd2mdu	384	28	cell	cell	NN
cord-014597-66vd2mdu	384	29	lines	line	NNS
cord-014597-66vd2mdu	384	30	and	and	CC
cord-014597-66vd2mdu	384	31	will	will	MD
cord-014597-66vd2mdu	384	32	need	need	VB
cord-014597-66vd2mdu	384	33	to	to	TO
cord-014597-66vd2mdu	384	34	be	be	VB
cord-014597-66vd2mdu	384	35	evaluated	evaluate	VBN
cord-014597-66vd2mdu	384	36	in	in	IN
cord-014597-66vd2mdu	384	37	future	future	JJ
cord-014597-66vd2mdu	384	38	studies	study	NNS
cord-014597-66vd2mdu	384	39	.	.	.
cord-014597-66vd2mdu	385	1	Feed	feed	NN
cord-014597-66vd2mdu	385	2	-	-	HYPH
cord-014597-66vd2mdu	385	3	spiking	spiking	NN
cord-014597-66vd2mdu	385	4	of	of	IN
cord-014597-66vd2mdu	385	5	basal	basal	NN
cord-014597-66vd2mdu	385	6	medium	medium	NN
cord-014597-66vd2mdu	385	7	is	be	VBZ
cord-014597-66vd2mdu	385	8	a	a	DT
cord-014597-66vd2mdu	385	9	convenient	convenient	JJ
cord-014597-66vd2mdu	385	10	and	and	CC
cord-014597-66vd2mdu	385	11	easy	easy	JJ
cord-014597-66vd2mdu	385	12	way	way	NN
cord-014597-66vd2mdu	385	13	to	to	TO
cord-014597-66vd2mdu	385	14	considerably	considerably	RB
cord-014597-66vd2mdu	385	15	increase	increase	VB
cord-014597-66vd2mdu	385	16	product	product	NN
cord-014597-66vd2mdu	385	17	concentrations	concentration	NNS
cord-014597-66vd2mdu	385	18	in	in	IN
cord-014597-66vd2mdu	385	19	a	a	DT
cord-014597-66vd2mdu	385	20	simple	simple	JJ
cord-014597-66vd2mdu	385	21	batch	batch	NN
cord-014597-66vd2mdu	385	22	culture	culture	NN
cord-014597-66vd2mdu	385	23	.	.	.
cord-014597-66vd2mdu	386	1	Differential	differential	JJ
cord-014597-66vd2mdu	386	2	gene	gene	NN
cord-014597-66vd2mdu	386	3	expression	expression	NN
cord-014597-66vd2mdu	386	4	revealed	reveal	VBD
cord-014597-66vd2mdu	386	5	genes	gene	NNS
cord-014597-66vd2mdu	386	6	that	that	WDT
cord-014597-66vd2mdu	386	7	appear	appear	VBP
cord-014597-66vd2mdu	386	8	important	important	JJ
cord-014597-66vd2mdu	386	9	for	for	IN
cord-014597-66vd2mdu	386	10	high	high	JJ
cord-014597-66vd2mdu	386	11	cell	cell	NN
cord-014597-66vd2mdu	386	12	-	-	HYPH
cord-014597-66vd2mdu	386	13	specific	specific	JJ
cord-014597-66vd2mdu	386	14	production	production	NN
cord-014597-66vd2mdu	386	15	rates	rate	NNS
cord-014597-66vd2mdu	386	16	,	,	,
cord-014597-66vd2mdu	386	17	and	and	CC
cord-014597-66vd2mdu	386	18	this	this	DT
cord-014597-66vd2mdu	386	19	knowledge	knowledge	NN
cord-014597-66vd2mdu	386	20	can	can	MD
cord-014597-66vd2mdu	386	21	be	be	VB
cord-014597-66vd2mdu	386	22	leveraged	leverage	VBN
cord-014597-66vd2mdu	386	23	into	into	IN
cord-014597-66vd2mdu	386	24	cell	cell	NN
cord-014597-66vd2mdu	386	25	line	line	NN
cord-014597-66vd2mdu	386	26	engineering	engineering	NN
cord-014597-66vd2mdu	386	27	approaches	approach	NNS
cord-014597-66vd2mdu	386	28	or	or	CC
cord-014597-66vd2mdu	386	29	the	the	DT
cord-014597-66vd2mdu	386	30	design	design	NN
cord-014597-66vd2mdu	386	31	of	of	IN
cord-014597-66vd2mdu	386	32	high	high	JJ
cord-014597-66vd2mdu	386	33	producing	produce	VBG
cord-014597-66vd2mdu	386	34	CHO	CHO	NNP
cord-014597-66vd2mdu	386	35	cell	cell	NN
cord-014597-66vd2mdu	386	36	media	medium	NNS
cord-014597-66vd2mdu	386	37	.	.	.
cord-014597-66vd2mdu	387	1	In	in	IN
cord-014597-66vd2mdu	387	2	the	the	DT
cord-014597-66vd2mdu	387	3	latter	latter	JJ
cord-014597-66vd2mdu	387	4	case	case	NN
cord-014597-66vd2mdu	387	5	,	,	,
cord-014597-66vd2mdu	387	6	a	a	DT
cord-014597-66vd2mdu	387	7	maximized	maximize	VBN
cord-014597-66vd2mdu	387	8	supply	supply	NN
cord-014597-66vd2mdu	387	9	of	of	IN
cord-014597-66vd2mdu	387	10	 	 	_SP
cord-014597-66vd2mdu	387	11	High	high	JJ
cord-014597-66vd2mdu	387	12	biosimilarity	biosimilarity	NN
cord-014597-66vd2mdu	387	13	must	must	MD
cord-014597-66vd2mdu	387	14	be	be	VB
cord-014597-66vd2mdu	387	15	demonstrated	demonstrate	VBN
cord-014597-66vd2mdu	387	16	by	by	IN
cord-014597-66vd2mdu	387	17	physicochemical	physicochemical	JJ
cord-014597-66vd2mdu	387	18	and	and	CC
cord-014597-66vd2mdu	387	19	functional	functional	JJ
cord-014597-66vd2mdu	387	20	characterization	characterization	NN
cord-014597-66vd2mdu	387	21	for	for	IN
cord-014597-66vd2mdu	387	22	approval	approval	NN
cord-014597-66vd2mdu	387	23	requirements	requirement	NNS
cord-014597-66vd2mdu	387	24	of	of	IN
cord-014597-66vd2mdu	387	25	phase	phase	NN
cord-014597-66vd2mdu	388	1	I	-PRON-	PRP
cord-014597-66vd2mdu	388	2	and	and	CC
cord-014597-66vd2mdu	388	3	phase	phase	NN
cord-014597-66vd2mdu	388	4	III	iii	CD
cord-014597-66vd2mdu	388	5	studies	study	NNS
cord-014597-66vd2mdu	388	6	in	in	IN
cord-014597-66vd2mdu	388	7	terms	term	NNS
cord-014597-66vd2mdu	388	8	of	of	IN
cord-014597-66vd2mdu	388	9	efficacy	efficacy	NN
cord-014597-66vd2mdu	388	10	,	,	,
cord-014597-66vd2mdu	388	11	safety	safety	NN
cord-014597-66vd2mdu	388	12	and	and	CC
cord-014597-66vd2mdu	388	13	immunogenicity	immunogenicity	NN
cord-014597-66vd2mdu	388	14	.	.	.
cord-014597-66vd2mdu	389	1	In	in	IN
cord-014597-66vd2mdu	389	2	this	this	DT
cord-014597-66vd2mdu	389	3	study	study	NN
cord-014597-66vd2mdu	389	4	,	,	,
cord-014597-66vd2mdu	389	5	rounds	round	NNS
cord-014597-66vd2mdu	389	6	of	of	IN
cord-014597-66vd2mdu	389	7	upstream	upstream	NN
cord-014597-66vd2mdu	389	8	and	and	CC
cord-014597-66vd2mdu	389	9	downstream	downstream	JJ
cord-014597-66vd2mdu	389	10	processes	process	NNS
cord-014597-66vd2mdu	389	11	were	be	VBD
cord-014597-66vd2mdu	389	12	run	run	VBN
cord-014597-66vd2mdu	389	13	to	to	TO
cord-014597-66vd2mdu	389	14	reach	reach	VB
cord-014597-66vd2mdu	389	15	the	the	DT
cord-014597-66vd2mdu	389	16	CQA	CQA	NNP
cord-014597-66vd2mdu	389	17	limits	limit	NNS
cord-014597-66vd2mdu	389	18	of	of	IN
cord-014597-66vd2mdu	389	19	the	the	DT
cord-014597-66vd2mdu	389	20	originator	originator	NN
cord-014597-66vd2mdu	389	21	molecule	molecule	NN
cord-014597-66vd2mdu	389	22	.	.	.
cord-014597-66vd2mdu	390	1	After	after	IN
cord-014597-66vd2mdu	390	2	conducting	conduct	VBG
cord-014597-66vd2mdu	390	3	many	many	JJ
cord-014597-66vd2mdu	390	4	different	different	JJ
cord-014597-66vd2mdu	390	5	development	development	NN
cord-014597-66vd2mdu	390	6	strategies	strategy	NNS
cord-014597-66vd2mdu	390	7	,	,	,
cord-014597-66vd2mdu	390	8	the	the	DT
cord-014597-66vd2mdu	390	9	mirror	mirror	NN
cord-014597-66vd2mdu	390	10	plot	plot	NN
cord-014597-66vd2mdu	390	11	images	image	NNS
cord-014597-66vd2mdu	390	12	of	of	IN
cord-014597-66vd2mdu	390	13	the	the	DT
cord-014597-66vd2mdu	390	14	intact	intact	JJ
cord-014597-66vd2mdu	390	15	deconvoluted	deconvoluted	JJ
cord-014597-66vd2mdu	390	16	mass	mass	NN
cord-014597-66vd2mdu	390	17	were	be	VBD
cord-014597-66vd2mdu	390	18	found	find	VBN
cord-014597-66vd2mdu	390	19	to	to	TO
cord-014597-66vd2mdu	390	20	be	be	VB
cord-014597-66vd2mdu	390	21	identical	identical	JJ
cord-014597-66vd2mdu	390	22	corresponding	correspond	VBG
cord-014597-66vd2mdu	390	23	to	to	IN
cord-014597-66vd2mdu	390	24	similar	similar	JJ
cord-014597-66vd2mdu	390	25	levels	level	NNS
cord-014597-66vd2mdu	390	26	of	of	IN
cord-014597-66vd2mdu	390	27	glycoforms	glycoform	NNS
cord-014597-66vd2mdu	390	28	.	.	.
cord-014597-66vd2mdu	391	1	The	the	DT
cord-014597-66vd2mdu	391	2	UV	uv	NN
cord-014597-66vd2mdu	391	3	chromatogram	chromatogram	NN
cord-014597-66vd2mdu	391	4	of	of	IN
cord-014597-66vd2mdu	391	5	reversed	reversed	JJ
cord-014597-66vd2mdu	391	6	phase	phase	NN
cord-014597-66vd2mdu	391	7	ultraperformance	ultraperformance	NN
cord-014597-66vd2mdu	391	8	liquid	liquid	NN
cord-014597-66vd2mdu	391	9	chromatography	chromatography	NN
cord-014597-66vd2mdu	391	10	(	(	-LRB-
cord-014597-66vd2mdu	391	11	RP	RP	NNP
cord-014597-66vd2mdu	391	12	-	-	HYPH
cord-014597-66vd2mdu	391	13	UPLC	UPLC	NNP
cord-014597-66vd2mdu	391	14	)	)	-RRB-
cord-014597-66vd2mdu	391	15	of	of	IN
cord-014597-66vd2mdu	391	16	tryptic	tryptic	JJ
cord-014597-66vd2mdu	391	17	peptide	peptide	NN
cord-014597-66vd2mdu	391	18	mapping	mapping	NN
cord-014597-66vd2mdu	391	19	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	391	20	that	that	IN
cord-014597-66vd2mdu	391	21	the	the	DT
cord-014597-66vd2mdu	391	22	primary	primary	JJ
cord-014597-66vd2mdu	391	23	structure	structure	NN
cord-014597-66vd2mdu	391	24	of	of	IN
cord-014597-66vd2mdu	391	25	TUR01	TUR01	NNP
cord-014597-66vd2mdu	391	26	is	be	VBZ
cord-014597-66vd2mdu	391	27	identical	identical	JJ
cord-014597-66vd2mdu	391	28	to	to	IN
cord-014597-66vd2mdu	391	29	the	the	DT
cord-014597-66vd2mdu	391	30	originator	originator	NN
cord-014597-66vd2mdu	391	31	as	as	IN
cord-014597-66vd2mdu	391	32	shown	show	VBN
cord-014597-66vd2mdu	391	33	in	in	IN
cord-014597-66vd2mdu	391	34	Fig	Fig	NNP
cord-014597-66vd2mdu	391	35	.	.	.
cord-014597-66vd2mdu	392	1	1a	1a	LS
cord-014597-66vd2mdu	392	2	.	.	.
cord-014597-66vd2mdu	393	1	Post	post	JJ
cord-014597-66vd2mdu	393	2	-	-	JJ
cord-014597-66vd2mdu	393	3	translational	translational	JJ
cord-014597-66vd2mdu	393	4	modifications	modification	NNS
cord-014597-66vd2mdu	393	5	(	(	-LRB-
cord-014597-66vd2mdu	393	6	PTMs	ptm	NNS
cord-014597-66vd2mdu	393	7	)	)	-RRB-
cord-014597-66vd2mdu	393	8	such	such	JJ
cord-014597-66vd2mdu	393	9	as	as	IN
cord-014597-66vd2mdu	393	10	oxidation	oxidation	NN
cord-014597-66vd2mdu	393	11	,	,	,
cord-014597-66vd2mdu	393	12	deamidation	deamidation	NN
cord-014597-66vd2mdu	393	13	,	,	,
cord-014597-66vd2mdu	393	14	N	n	CD
cord-014597-66vd2mdu	393	15	-	-	HYPH
cord-014597-66vd2mdu	393	16	terminal	terminal	JJ
cord-014597-66vd2mdu	393	17	pyroglutamic	pyroglutamic	JJ
cord-014597-66vd2mdu	393	18	acid	acid	NN
cord-014597-66vd2mdu	393	19	,	,	,
cord-014597-66vd2mdu	393	20	C	c	NN
cord-014597-66vd2mdu	393	21	-	-	HYPH
cord-014597-66vd2mdu	393	22	terminal	terminal	NN
cord-014597-66vd2mdu	393	23	lysine	lysine	NN
cord-014597-66vd2mdu	393	24	truncation	truncation	NN
cord-014597-66vd2mdu	393	25	levels	level	NNS
cord-014597-66vd2mdu	393	26	were	be	VBD
cord-014597-66vd2mdu	393	27	also	also	RB
cord-014597-66vd2mdu	393	28	comparable	comparable	JJ
cord-014597-66vd2mdu	393	29	for	for	IN
cord-014597-66vd2mdu	393	30	two	two	CD
cord-014597-66vd2mdu	393	31	products	product	NNS
cord-014597-66vd2mdu	393	32	.	.	.
cord-014597-66vd2mdu	394	1	The	the	DT
cord-014597-66vd2mdu	394	2	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	394	3	site	site	NN
cord-014597-66vd2mdu	394	4	(	(	-LRB-
cord-014597-66vd2mdu	394	5	HC	HC	NNP
cord-014597-66vd2mdu	394	6	-	-	HYPH
cord-014597-66vd2mdu	394	7	Asn301	Asn301	NNP
cord-014597-66vd2mdu	394	8	)	)	-RRB-
cord-014597-66vd2mdu	394	9	was	be	VBD
cord-014597-66vd2mdu	394	10	confirmed	confirm	VBN
cord-014597-66vd2mdu	394	11	by	by	IN
cord-014597-66vd2mdu	394	12	peptide	peptide	NN
cord-014597-66vd2mdu	394	13	mapping	mapping	NN
cord-014597-66vd2mdu	394	14	analysis	analysis	NN
cord-014597-66vd2mdu	394	15	and	and	CC
cord-014597-66vd2mdu	394	16	100	100	CD
cord-014597-66vd2mdu	394	17	%	%	NN
cord-014597-66vd2mdu	394	18	glycan	glycan	NN
cord-014597-66vd2mdu	394	19	site	site	NN
cord-014597-66vd2mdu	394	20	occupancy	occupancy	NN
cord-014597-66vd2mdu	394	21	was	be	VBD
cord-014597-66vd2mdu	394	22	proven	prove	VBN
cord-014597-66vd2mdu	394	23	for	for	IN
cord-014597-66vd2mdu	394	24	TUR01	TUR01	NNP
cord-014597-66vd2mdu	394	25	and	and	CC
cord-014597-66vd2mdu	394	26	originator	originator	NN
cord-014597-66vd2mdu	394	27	.	.	.
cord-014597-66vd2mdu	395	1	The	the	DT
cord-014597-66vd2mdu	395	2	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	395	3	pattern	pattern	NN
cord-014597-66vd2mdu	395	4	for	for	IN
cord-014597-66vd2mdu	395	5	two	two	CD
cord-014597-66vd2mdu	395	6	products	product	NNS
cord-014597-66vd2mdu	395	7	were	be	VBD
cord-014597-66vd2mdu	395	8	highly	highly	RB
cord-014597-66vd2mdu	395	9	similar	similar	JJ
cord-014597-66vd2mdu	395	10	in	in	IN
cord-014597-66vd2mdu	395	11	terms	term	NNS
cord-014597-66vd2mdu	395	12	of	of	IN
cord-014597-66vd2mdu	395	13	major	major	JJ
cord-014597-66vd2mdu	395	14	glycans	glycan	NNS
cord-014597-66vd2mdu	395	15	(	(	-LRB-
cord-014597-66vd2mdu	395	16	G0F	G0F	NNS
cord-014597-66vd2mdu	395	17	,	,	,
cord-014597-66vd2mdu	395	18	G1F	g1f	RB
cord-014597-66vd2mdu	395	19	,	,	,
cord-014597-66vd2mdu	395	20	G0F	g0f	NN
cord-014597-66vd2mdu	395	21	-	-	HYPH
cord-014597-66vd2mdu	395	22	GN	gn	NN
cord-014597-66vd2mdu	395	23	and	and	CC
cord-014597-66vd2mdu	395	24	etc	etc	NN
cord-014597-66vd2mdu	395	25	.	.	.
cord-014597-66vd2mdu	395	26	)	)	-RRB-
cord-014597-66vd2mdu	395	27	.	.	.
cord-014597-66vd2mdu	396	1	Man5	man5	JJ
cord-014597-66vd2mdu	396	2	level	level	NN
cord-014597-66vd2mdu	396	3	was	be	VBD
cord-014597-66vd2mdu	396	4	lower	low	JJR
cord-014597-66vd2mdu	396	5	in	in	IN
cord-014597-66vd2mdu	396	6	TUR01	TUR01	NNS
cord-014597-66vd2mdu	396	7	compared	compare	VBN
cord-014597-66vd2mdu	396	8	to	to	IN
cord-014597-66vd2mdu	396	9	the	the	DT
cord-014597-66vd2mdu	396	10	originator	originator	NN
cord-014597-66vd2mdu	396	11	product	product	NN
cord-014597-66vd2mdu	396	12	which	which	WDT
cord-014597-66vd2mdu	396	13	may	may	MD
cord-014597-66vd2mdu	396	14	not	not	RB
cord-014597-66vd2mdu	396	15	have	have	VB
cord-014597-66vd2mdu	396	16	any	any	DT
cord-014597-66vd2mdu	396	17	clinical	clinical	JJ
cord-014597-66vd2mdu	396	18	effect	effect	NN
cord-014597-66vd2mdu	396	19	on	on	IN
cord-014597-66vd2mdu	396	20	the	the	DT
cord-014597-66vd2mdu	396	21	molecule	molecule	NN
cord-014597-66vd2mdu	396	22	.	.	.
cord-014597-66vd2mdu	397	1	The	the	DT
cord-014597-66vd2mdu	397	2	secondary	secondary	JJ
cord-014597-66vd2mdu	397	3	structure	structure	NN
cord-014597-66vd2mdu	397	4	was	be	VBD
cord-014597-66vd2mdu	397	5	determined	determine	VBN
cord-014597-66vd2mdu	397	6	by	by	IN
cord-014597-66vd2mdu	397	7	ATR	ATR	NNP
cord-014597-66vd2mdu	397	8	-	-	HYPH
cord-014597-66vd2mdu	397	9	FTIR	FTIR	NNP
cord-014597-66vd2mdu	397	10	spectroscopy	spectroscopy	NN
cord-014597-66vd2mdu	397	11	.	.	.
cord-014597-66vd2mdu	398	1	Absorption	absorption	NN
cord-014597-66vd2mdu	398	2	bands	band	NNS
cord-014597-66vd2mdu	398	3	(	(	-LRB-
cord-014597-66vd2mdu	398	4	Amide	Amide	NNP
cord-014597-66vd2mdu	398	5	I	I	NNP
cord-014597-66vd2mdu	398	6	and	and	CC
cord-014597-66vd2mdu	398	7	Amide	Amide	NNP
cord-014597-66vd2mdu	398	8	II	II	NNP
cord-014597-66vd2mdu	398	9	)	)	-RRB-
cord-014597-66vd2mdu	398	10	were	be	VBD
cord-014597-66vd2mdu	398	11	overlapped	overlap	VBN
cord-014597-66vd2mdu	398	12	completely	completely	RB
cord-014597-66vd2mdu	398	13	and	and	CC
cord-014597-66vd2mdu	398	14	amounts	amount	NNS
cord-014597-66vd2mdu	398	15	of	of	IN
cord-014597-66vd2mdu	398	16	α	α	NN
cord-014597-66vd2mdu	398	17	-	-	HYPH
cord-014597-66vd2mdu	398	18	helix	helix	NN
cord-014597-66vd2mdu	398	19	and	and	CC
cord-014597-66vd2mdu	398	20	β	β	NN
cord-014597-66vd2mdu	398	21	-	-	HYPH
cord-014597-66vd2mdu	398	22	sheet	sheet	NN
cord-014597-66vd2mdu	398	23	structures	structure	NNS
cord-014597-66vd2mdu	398	24	were	be	VBD
cord-014597-66vd2mdu	398	25	comparable	comparable	JJ
cord-014597-66vd2mdu	398	26	.	.	.
cord-014597-66vd2mdu	399	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	399	2	;	;	:
cord-014597-66vd2mdu	399	3	size	size	NN
cord-014597-66vd2mdu	399	4	-	-	HYPH
cord-014597-66vd2mdu	399	5	exclusion	exclusion	NN
cord-014597-66vd2mdu	399	6	chromatography	chromatography	NN
cord-014597-66vd2mdu	399	7	(	(	-LRB-
cord-014597-66vd2mdu	399	8	SEC	SEC	NNP
cord-014597-66vd2mdu	399	9	)	)	-RRB-
cord-014597-66vd2mdu	399	10	analysis	analysis	NN
cord-014597-66vd2mdu	399	11	revealed	reveal	VBD
cord-014597-66vd2mdu	399	12	that	that	IN
cord-014597-66vd2mdu	399	13	both	both	DT
cord-014597-66vd2mdu	399	14	products	product	NNS
cord-014597-66vd2mdu	399	15	have	have	VBP
cord-014597-66vd2mdu	399	16	the	the	DT
cord-014597-66vd2mdu	399	17	same	same	JJ
cord-014597-66vd2mdu	399	18	level	level	NN
cord-014597-66vd2mdu	399	19	of	of	IN
cord-014597-66vd2mdu	399	20	purity	purity	NN
cord-014597-66vd2mdu	399	21	(	(	-LRB-
cord-014597-66vd2mdu	399	22	>	>	NFP
cord-014597-66vd2mdu	399	23	99	99	CD
cord-014597-66vd2mdu	399	24	%	%	NN
cord-014597-66vd2mdu	399	25	)	)	-RRB-
cord-014597-66vd2mdu	399	26	and	and	CC
cord-014597-66vd2mdu	399	27	aggregate	aggregate	NN
cord-014597-66vd2mdu	399	28	(	(	-LRB-
cord-014597-66vd2mdu	399	29	<	<	NN
cord-014597-66vd2mdu	399	30	1	1	CD
cord-014597-66vd2mdu	399	31	%	%	NN
cord-014597-66vd2mdu	399	32	)	)	-RRB-
cord-014597-66vd2mdu	399	33	levels	level	NNS
cord-014597-66vd2mdu	399	34	.	.	.
cord-014597-66vd2mdu	400	1	The	the	DT
cord-014597-66vd2mdu	400	2	level	level	NN
cord-014597-66vd2mdu	400	3	of	of	IN
cord-014597-66vd2mdu	400	4	impurities	impurity	NNS
cord-014597-66vd2mdu	400	5	were	be	VBD
cord-014597-66vd2mdu	400	6	determined	determine	VBN
cord-014597-66vd2mdu	400	7	as	as	IN
cord-014597-66vd2mdu	400	8	below	below	IN
cord-014597-66vd2mdu	400	9	4	4	CD
cord-014597-66vd2mdu	400	10	%	%	NN
cord-014597-66vd2mdu	400	11	by	by	IN
cord-014597-66vd2mdu	400	12	CE	CE	NNP
cord-014597-66vd2mdu	400	13	-	-	HYPH
cord-014597-66vd2mdu	400	14	SDS	SDS	NNP
cord-014597-66vd2mdu	400	15	.	.	.
cord-014597-66vd2mdu	401	1	The	the	DT
cord-014597-66vd2mdu	401	2	capillary	capillary	NN
cord-014597-66vd2mdu	401	3	isoelectric	isoelectric	JJ
cord-014597-66vd2mdu	401	4	focusing	focus	VBG
cord-014597-66vd2mdu	401	5	(	(	-LRB-
cord-014597-66vd2mdu	402	1	cIEF	cief	LS
cord-014597-66vd2mdu	402	2	)	)	-RRB-
cord-014597-66vd2mdu	402	3	experiments	experiment	NNS
cord-014597-66vd2mdu	402	4	showed	show	VBD
cord-014597-66vd2mdu	402	5	that	that	IN
cord-014597-66vd2mdu	402	6	the	the	DT
cord-014597-66vd2mdu	402	7	charge	charge	NN
cord-014597-66vd2mdu	402	8	variant	variant	NN
cord-014597-66vd2mdu	402	9	profiles	profile	NNS
cord-014597-66vd2mdu	402	10	of	of	IN
cord-014597-66vd2mdu	402	11	two	two	CD
cord-014597-66vd2mdu	402	12	products	product	NNS
cord-014597-66vd2mdu	402	13	are	be	VBP
cord-014597-66vd2mdu	402	14	indistinguishable	indistinguishable	JJ
cord-014597-66vd2mdu	402	15	and	and	CC
cord-014597-66vd2mdu	402	16	the	the	DT
cord-014597-66vd2mdu	402	17	isoelectric	isoelectric	JJ
cord-014597-66vd2mdu	402	18	point	point	NN
cord-014597-66vd2mdu	402	19	of	of	IN
cord-014597-66vd2mdu	402	20	main	main	JJ
cord-014597-66vd2mdu	402	21	peak	peak	NN
cord-014597-66vd2mdu	402	22	is	be	VBZ
cord-014597-66vd2mdu	402	23	observed	observe	VBN
cord-014597-66vd2mdu	402	24	at	at	IN
cord-014597-66vd2mdu	402	25	8.3	8.3	CD
cord-014597-66vd2mdu	402	26	for	for	IN
cord-014597-66vd2mdu	402	27	both	both	DT
cord-014597-66vd2mdu	402	28	products	product	NNS
cord-014597-66vd2mdu	402	29	.	.	.
cord-014597-66vd2mdu	403	1	The	the	DT
cord-014597-66vd2mdu	403	2	association	association	NN
cord-014597-66vd2mdu	403	3	/	/	SYM
cord-014597-66vd2mdu	403	4	dissociation	dissociation	NN
cord-014597-66vd2mdu	403	5	rate	rate	NN
cord-014597-66vd2mdu	403	6	constants	constant	NNS
cord-014597-66vd2mdu	403	7	and	and	CC
cord-014597-66vd2mdu	403	8	binding	bind	VBG
cord-014597-66vd2mdu	403	9	affinity	affinity	NN
cord-014597-66vd2mdu	403	10	for	for	IN
cord-014597-66vd2mdu	403	11	both	both	CC
cord-014597-66vd2mdu	403	12	TUR01	TUR01	NNP
cord-014597-66vd2mdu	403	13	and	and	CC
cord-014597-66vd2mdu	403	14	originator	originator	NN
cord-014597-66vd2mdu	403	15	were	be	VBD
cord-014597-66vd2mdu	403	16	highly	highly	RB
cord-014597-66vd2mdu	403	17	similar	similar	JJ
cord-014597-66vd2mdu	403	18	and	and	CC
cord-014597-66vd2mdu	403	19	similarity	similarity	NN
cord-014597-66vd2mdu	403	20	score	score	NN
cord-014597-66vd2mdu	403	21	was	be	VBD
cord-014597-66vd2mdu	403	22	calculated	calculate	VBN
cord-014597-66vd2mdu	403	23	greater	great	JJR
cord-014597-66vd2mdu	403	24	than	than	IN
cord-014597-66vd2mdu	403	25	99	99	CD
cord-014597-66vd2mdu	403	26	%	%	NN
cord-014597-66vd2mdu	403	27	,	,	,
cord-014597-66vd2mdu	403	28	as	as	IN
cord-014597-66vd2mdu	403	29	shown	show	VBN
cord-014597-66vd2mdu	403	30	in	in	IN
cord-014597-66vd2mdu	403	31	Fig	Fig	NNP
cord-014597-66vd2mdu	403	32	.	.	.
cord-014597-66vd2mdu	404	1	1b	1b	LS
cord-014597-66vd2mdu	404	2	.	.	.
cord-014597-66vd2mdu	405	1	In	in	IN
cord-014597-66vd2mdu	405	2	this	this	DT
cord-014597-66vd2mdu	405	3	study	study	NN
cord-014597-66vd2mdu	405	4	,	,	,
cord-014597-66vd2mdu	405	5	state	state	NN
cord-014597-66vd2mdu	405	6	-	-	HYPH
cord-014597-66vd2mdu	405	7	of	of	IN
cord-014597-66vd2mdu	405	8	-	-	HYPH
cord-014597-66vd2mdu	405	9	art	art	NN
cord-014597-66vd2mdu	405	10	analytical	analytical	JJ
cord-014597-66vd2mdu	405	11	techniques	technique	NNS
cord-014597-66vd2mdu	405	12	were	be	VBD
cord-014597-66vd2mdu	405	13	used	use	VBN
cord-014597-66vd2mdu	405	14	to	to	TO
cord-014597-66vd2mdu	405	15	assess	assess	VB
cord-014597-66vd2mdu	405	16	the	the	DT
cord-014597-66vd2mdu	405	17	biosimilarity	biosimilarity	NN
cord-014597-66vd2mdu	405	18	of	of	IN
cord-014597-66vd2mdu	405	19	TUR01	TUR01	NNP
cord-014597-66vd2mdu	405	20	to	to	IN
cord-014597-66vd2mdu	405	21	the	the	DT
cord-014597-66vd2mdu	405	22	originator	originator	NN
cord-014597-66vd2mdu	405	23	adalimumab	adalimumab	NNS
cord-014597-66vd2mdu	405	24	.	.	.
cord-014597-66vd2mdu	406	1	Head	Head	NNP
cord-014597-66vd2mdu	406	2	-	-	HYPH
cord-014597-66vd2mdu	406	3	tohead	tohead	JJ
cord-014597-66vd2mdu	406	4	comparison	comparison	NN
cord-014597-66vd2mdu	406	5	data	datum	NNS
cord-014597-66vd2mdu	406	6	clearly	clearly	RB
cord-014597-66vd2mdu	406	7	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	406	8	that	that	IN
cord-014597-66vd2mdu	406	9	TUR01	TUR01	NNP
cord-014597-66vd2mdu	406	10	is	be	VBZ
cord-014597-66vd2mdu	406	11	highly	highly	RB
cord-014597-66vd2mdu	406	12	similar	similar	JJ
cord-014597-66vd2mdu	406	13	to	to	IN
cord-014597-66vd2mdu	406	14	the	the	DT
cord-014597-66vd2mdu	406	15	originator	originator	NN
cord-014597-66vd2mdu	406	16	adalimumab	adalimumab	NNS
cord-014597-66vd2mdu	406	17	in	in	IN
cord-014597-66vd2mdu	406	18	terms	term	NNS
cord-014597-66vd2mdu	406	19	of	of	IN
cord-014597-66vd2mdu	406	20	physicochemical	physicochemical	JJ
cord-014597-66vd2mdu	406	21	and	and	CC
cord-014597-66vd2mdu	406	22	functional	functional	JJ
cord-014597-66vd2mdu	406	23	characteristics	characteristic	NNS
cord-014597-66vd2mdu	406	24	.	.	.
cord-014597-66vd2mdu	407	1	Based	base	VBN
cord-014597-66vd2mdu	407	2	on	on	IN
cord-014597-66vd2mdu	407	3	the	the	DT
cord-014597-66vd2mdu	407	4	analytical	analytical	JJ
cord-014597-66vd2mdu	407	5	similarities	similarity	NNS
cord-014597-66vd2mdu	407	6	,	,	,
cord-014597-66vd2mdu	407	7	we	-PRON-	PRP
cord-014597-66vd2mdu	407	8	.	.	.
cord-014597-66vd2mdu	408	1	Process	process	NN
cord-014597-66vd2mdu	408	2	performance	performance	NN
cord-014597-66vd2mdu	408	3	of	of	IN
cord-014597-66vd2mdu	408	4	basal	basal	NN
cord-014597-66vd2mdu	408	5	medium	medium	NN
cord-014597-66vd2mdu	408	6	(	(	-LRB-
cord-014597-66vd2mdu	408	7	black	black	JJ
cord-014597-66vd2mdu	408	8	)	)	-RRB-
cord-014597-66vd2mdu	408	9	and	and	CC
cord-014597-66vd2mdu	408	10	feed	feed	NN
cord-014597-66vd2mdu	408	11	-	-	HYPH
cord-014597-66vd2mdu	408	12	spiked	spiked	JJ
cord-014597-66vd2mdu	408	13	(	(	-LRB-
cord-014597-66vd2mdu	408	14	red	red	JJ
cord-014597-66vd2mdu	408	15	)	)	-RRB-
cord-014597-66vd2mdu	408	16	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	408	17	batch	batch	NN
cord-014597-66vd2mdu	408	18	cultures	culture	NNS
cord-014597-66vd2mdu	408	19	:	:	:
cord-014597-66vd2mdu	408	20	a	a	DT
cord-014597-66vd2mdu	408	21	cell	cell	NN
cord-014597-66vd2mdu	408	22	concentrations	concentration	NNS
cord-014597-66vd2mdu	408	23	and	and	CC
cord-014597-66vd2mdu	408	24	viability	viability	NN
cord-014597-66vd2mdu	408	25	,	,	,
cord-014597-66vd2mdu	408	26	b	b	LS
cord-014597-66vd2mdu	408	27	viable	viable	JJ
cord-014597-66vd2mdu	408	28	cumulative	cumulative	JJ
cord-014597-66vd2mdu	408	29	cell	cell	NN
cord-014597-66vd2mdu	408	30	days	day	NNS
cord-014597-66vd2mdu	408	31	and	and	CC
cord-014597-66vd2mdu	408	32	specific	specific	JJ
cord-014597-66vd2mdu	408	33	growth	growth	NN
cord-014597-66vd2mdu	408	34	rate	rate	NN
cord-014597-66vd2mdu	408	35	,	,	,
cord-014597-66vd2mdu	408	36	and	and	CC
cord-014597-66vd2mdu	408	37	c	c	NN
cord-014597-66vd2mdu	408	38	antibody	antibody	NN
cord-014597-66vd2mdu	408	39	concentrations	concentration	NNS
cord-014597-66vd2mdu	408	40	and	and	CC
cord-014597-66vd2mdu	408	41	cell	cell	NN
cord-014597-66vd2mdu	408	42	-	-	HYPH
cord-014597-66vd2mdu	408	43	specific	specific	JJ
cord-014597-66vd2mdu	408	44	productivity	productivity	NN
cord-014597-66vd2mdu	408	45	.	.	.
cord-014597-66vd2mdu	409	1	Error	error	NN
cord-014597-66vd2mdu	409	2	bars	bar	NNS
cord-014597-66vd2mdu	409	3	indicate	indicate	VBP
cord-014597-66vd2mdu	409	4	standard	standard	JJ
cord-014597-66vd2mdu	409	5	deviation	deviation	NN
cord-014597-66vd2mdu	409	6	from	from	IN
cord-014597-66vd2mdu	409	7	three	three	CD
cord-014597-66vd2mdu	409	8	independent	independent	JJ
cord-014597-66vd2mdu	409	9	experiments	experiment	NNS
cord-014597-66vd2mdu	409	10	.	.	.
cord-014597-66vd2mdu	410	1	The	the	DT
cord-014597-66vd2mdu	410	2	black	black	JJ
cord-014597-66vd2mdu	410	3	arrows	arrow	NNS
cord-014597-66vd2mdu	410	4	on	on	IN
cord-014597-66vd2mdu	410	5	Day	Day	NNP
cord-014597-66vd2mdu	410	6	4	4	CD
cord-014597-66vd2mdu	410	7	indicate	indicate	VBP
cord-014597-66vd2mdu	410	8	the	the	DT
cord-014597-66vd2mdu	410	9	beginning	beginning	NN
cord-014597-66vd2mdu	410	10	of	of	IN
cord-014597-66vd2mdu	410	11	decreasing	decrease	VBG
cord-014597-66vd2mdu	410	12	cell	cell	NN
cord-014597-66vd2mdu	410	13	-	-	HYPH
cord-014597-66vd2mdu	410	14	specific	specific	JJ
cord-014597-66vd2mdu	410	15	productivities	productivity	NNS
cord-014597-66vd2mdu	410	16	and	and	CC
cord-014597-66vd2mdu	410	17	lower	low	JJR
cord-014597-66vd2mdu	410	18	cell	cell	NN
cord-014597-66vd2mdu	410	19	-	-	HYPH
cord-014597-66vd2mdu	410	20	specific	specific	JJ
cord-014597-66vd2mdu	410	21	growth	growth	NN
cord-014597-66vd2mdu	410	22	rates	rate	NNS
cord-014597-66vd2mdu	410	23	in	in	IN
cord-014597-66vd2mdu	410	24	basal	basal	NN
cord-014597-66vd2mdu	410	25	medium	medium	NN
cord-014597-66vd2mdu	410	26	cultures	culture	NNS
cord-014597-66vd2mdu	410	27	believe	believe	VBP
cord-014597-66vd2mdu	410	28	that	that	IN
cord-014597-66vd2mdu	410	29	TUR01	TUR01	NNP
cord-014597-66vd2mdu	410	30	will	will	MD
cord-014597-66vd2mdu	410	31	have	have	VB
cord-014597-66vd2mdu	410	32	comparable	comparable	JJ
cord-014597-66vd2mdu	410	33	PK	PK	NNP
cord-014597-66vd2mdu	410	34	/	/	SYM
cord-014597-66vd2mdu	410	35	PD	PD	NNP
cord-014597-66vd2mdu	410	36	,	,	,
cord-014597-66vd2mdu	410	37	potency	potency	NN
cord-014597-66vd2mdu	410	38	,	,	,
cord-014597-66vd2mdu	410	39	and	and	CC
cord-014597-66vd2mdu	410	40	efficacy	efficacy	NN
cord-014597-66vd2mdu	410	41	results	result	NNS
cord-014597-66vd2mdu	410	42	to	to	IN
cord-014597-66vd2mdu	410	43	the	the	DT
cord-014597-66vd2mdu	410	44	originator	originator	NN
cord-014597-66vd2mdu	410	45	adalimumab	adalimumab	NNS
cord-014597-66vd2mdu	410	46	.	.	.
cord-014597-66vd2mdu	411	1	The	the	DT
cord-014597-66vd2mdu	411	2	expanded	expand	VBN
cord-014597-66vd2mdu	411	3	interest	interest	NN
cord-014597-66vd2mdu	411	4	in	in	IN
cord-014597-66vd2mdu	411	5	intensified	intensify	VBN
cord-014597-66vd2mdu	411	6	continuous	continuous	JJ
cord-014597-66vd2mdu	411	7	bioprocessing	bioprocessing	NN
cord-014597-66vd2mdu	411	8	has	have	VBZ
cord-014597-66vd2mdu	411	9	highlighted	highlight	VBN
cord-014597-66vd2mdu	411	10	the	the	DT
cord-014597-66vd2mdu	411	11	need	need	NN
cord-014597-66vd2mdu	411	12	to	to	TO
cord-014597-66vd2mdu	411	13	develop	develop	VB
cord-014597-66vd2mdu	411	14	a	a	DT
cord-014597-66vd2mdu	411	15	small	small	JJ
cord-014597-66vd2mdu	411	16	scale	scale	NN
cord-014597-66vd2mdu	411	17	model	model	NN
cord-014597-66vd2mdu	411	18	for	for	IN
cord-014597-66vd2mdu	411	19	perfusion	perfusion	NN
cord-014597-66vd2mdu	411	20	cell	cell	NN
cord-014597-66vd2mdu	411	21	culture	culture	NN
cord-014597-66vd2mdu	411	22	.	.	.
cord-014597-66vd2mdu	412	1	The	the	DT
cord-014597-66vd2mdu	412	2	direction	direction	NN
cord-014597-66vd2mdu	412	3	in	in	IN
cord-014597-66vd2mdu	412	4	the	the	DT
cord-014597-66vd2mdu	412	5	industry	industry	NN
cord-014597-66vd2mdu	412	6	has	have	VBZ
cord-014597-66vd2mdu	412	7	been	be	VBN
cord-014597-66vd2mdu	412	8	to	to	TO
cord-014597-66vd2mdu	412	9	increase	increase	VB
cord-014597-66vd2mdu	412	10	target	target	NN
cord-014597-66vd2mdu	412	11	cell	cell	NN
cord-014597-66vd2mdu	412	12	densities	density	NNS
cord-014597-66vd2mdu	412	13	to	to	IN
cord-014597-66vd2mdu	412	14	≥50x10	≥50x10	NN
cord-014597-66vd2mdu	412	15	6	6	CD
cord-014597-66vd2mdu	412	16	vc	vc	NNP
cord-014597-66vd2mdu	412	17	/	/	SYM
cord-014597-66vd2mdu	412	18	mL	ml	NN
cord-014597-66vd2mdu	412	19	and	and	CC
cord-014597-66vd2mdu	412	20	decrease	decrease	VB
cord-014597-66vd2mdu	412	21	perfusion	perfusion	NN
cord-014597-66vd2mdu	412	22	rates	rate	NNS
cord-014597-66vd2mdu	412	23	to	to	IN
cord-014597-66vd2mdu	412	24	≤3vvd	≤3vvd	NNP
cord-014597-66vd2mdu	412	25	.	.	.
cord-014597-66vd2mdu	413	1	In	in	IN
cord-014597-66vd2mdu	413	2	order	order	NN
cord-014597-66vd2mdu	413	3	to	to	TO
cord-014597-66vd2mdu	413	4	increase	increase	VB
cord-014597-66vd2mdu	413	5	the	the	DT
cord-014597-66vd2mdu	413	6	throughput	throughput	NN
cord-014597-66vd2mdu	413	7	of	of	IN
cord-014597-66vd2mdu	413	8	our	-PRON-	PRP$
cord-014597-66vd2mdu	413	9	perfusion	perfusion	NN
cord-014597-66vd2mdu	413	10	media	media	NN
cord-014597-66vd2mdu	413	11	development	development	NN
cord-014597-66vd2mdu	413	12	capabilities	capability	NNS
cord-014597-66vd2mdu	413	13	we	-PRON-	PRP
cord-014597-66vd2mdu	413	14	sought	seek	VBD
cord-014597-66vd2mdu	413	15	to	to	TO
cord-014597-66vd2mdu	413	16	develop	develop	VB
cord-014597-66vd2mdu	413	17	a	a	DT
cord-014597-66vd2mdu	413	18	small	small	JJ
cord-014597-66vd2mdu	413	19	scale	scale	NN
cord-014597-66vd2mdu	413	20	model	model	NN
cord-014597-66vd2mdu	413	21	of	of	IN
cord-014597-66vd2mdu	413	22	perfusion	perfusion	NN
cord-014597-66vd2mdu	413	23	using	use	VBG
cord-014597-66vd2mdu	413	24	the	the	DT
cord-014597-66vd2mdu	413	25	ambr	ambr	NNP
cord-014597-66vd2mdu	413	26	®	®	NN
cord-014597-66vd2mdu	413	27	15	15	CD
cord-014597-66vd2mdu	413	28	instrument	instrument	NN
cord-014597-66vd2mdu	413	29	(	(	-LRB-
cord-014597-66vd2mdu	413	30	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	413	31	,	,	,
cord-014597-66vd2mdu	413	32	Germany	Germany	NNP
cord-014597-66vd2mdu	413	33	)	)	-RRB-
cord-014597-66vd2mdu	413	34	.	.	.
cord-014597-66vd2mdu	414	1	We	-PRON-	PRP
cord-014597-66vd2mdu	414	2	used	use	VBD
cord-014597-66vd2mdu	414	3	a	a	DT
cord-014597-66vd2mdu	414	4	modified	modify	VBN
cord-014597-66vd2mdu	414	5	cell	cell	NN
cord-014597-66vd2mdu	414	6	settling	settling	NN
cord-014597-66vd2mdu	414	7	model	model	NN
cord-014597-66vd2mdu	414	8	from	from	IN
cord-014597-66vd2mdu	414	9	the	the	DT
cord-014597-66vd2mdu	414	10	previously	previously	RB
cord-014597-66vd2mdu	414	11	published	publish	VBN
cord-014597-66vd2mdu	414	12	by	by	IN
cord-014597-66vd2mdu	414	13	Kreye	Kreye	NNP
cord-014597-66vd2mdu	414	14	et	et	NNP
cord-014597-66vd2mdu	414	15	.	.	.
cord-014597-66vd2mdu	415	1	al	al	NNP
cord-014597-66vd2mdu	415	2	.	.	.
cord-014597-66vd2mdu	416	1	to	to	TO
cord-014597-66vd2mdu	416	2	achieve	achieve	VB
cord-014597-66vd2mdu	416	3	the	the	DT
cord-014597-66vd2mdu	416	4	cell	cell	NN
cord-014597-66vd2mdu	416	5	retention	retention	NN
cord-014597-66vd2mdu	416	6	necessary	necessary	JJ
cord-014597-66vd2mdu	416	7	to	to	TO
cord-014597-66vd2mdu	416	8	reach	reach	VB
cord-014597-66vd2mdu	416	9	perfusion	perfusion	NN
cord-014597-66vd2mdu	416	10	relevant	relevant	JJ
cord-014597-66vd2mdu	416	11	viable	viable	JJ
cord-014597-66vd2mdu	416	12	cell	cell	NN
cord-014597-66vd2mdu	416	13	concentrations	concentration	NNS
cord-014597-66vd2mdu	416	14	[	[	-LRB-
cord-014597-66vd2mdu	416	15	1	1	CD
cord-014597-66vd2mdu	416	16	]	]	-RRB-
cord-014597-66vd2mdu	416	17	.	.	.
cord-014597-66vd2mdu	417	1	In	in	IN
cord-014597-66vd2mdu	417	2	this	this	DT
cord-014597-66vd2mdu	417	3	work	work	NN
cord-014597-66vd2mdu	417	4	,	,	,
cord-014597-66vd2mdu	417	5	we	-PRON-	PRP
cord-014597-66vd2mdu	417	6	will	will	MD
cord-014597-66vd2mdu	417	7	show	show	VB
cord-014597-66vd2mdu	417	8	the	the	DT
cord-014597-66vd2mdu	417	9	application	application	NN
cord-014597-66vd2mdu	417	10	of	of	IN
cord-014597-66vd2mdu	417	11	this	this	DT
cord-014597-66vd2mdu	417	12	small	small	JJ
cord-014597-66vd2mdu	417	13	scale	scale	NN
cord-014597-66vd2mdu	417	14	model	model	NN
cord-014597-66vd2mdu	417	15	for	for	IN
cord-014597-66vd2mdu	417	16	:	:	:
cord-014597-66vd2mdu	418	1	(	(	-LRB-
cord-014597-66vd2mdu	418	2	1	1	LS
cord-014597-66vd2mdu	418	3	)	)	-RRB-
cord-014597-66vd2mdu	418	4	Identification	identification	NN
cord-014597-66vd2mdu	418	5	of	of	IN
cord-014597-66vd2mdu	418	6	specific	specific	JJ
cord-014597-66vd2mdu	418	7	productivity	productivity	NN
cord-014597-66vd2mdu	418	8	performance	performance	NN
cord-014597-66vd2mdu	418	9	over	over	IN
cord-014597-66vd2mdu	418	10	a	a	DT
cord-014597-66vd2mdu	418	11	steady	steady	JJ
cord-014597-66vd2mdu	418	12	-	-	HYPH
cord-014597-66vd2mdu	418	13	state	state	NN
cord-014597-66vd2mdu	418	14	for	for	IN
cord-014597-66vd2mdu	418	15	tested	test	VBN
cord-014597-66vd2mdu	418	16	media	medium	NNS
cord-014597-66vd2mdu	418	17	,	,	,
cord-014597-66vd2mdu	418	18	(	(	-LRB-
cord-014597-66vd2mdu	418	19	2	2	LS
cord-014597-66vd2mdu	418	20	)	)	-RRB-
cord-014597-66vd2mdu	418	21	Identification	identification	NN
cord-014597-66vd2mdu	418	22	of	of	IN
cord-014597-66vd2mdu	418	23	CSPR	CSPR	NNP
cord-014597-66vd2mdu	418	24	min	min	NNP
cord-014597-66vd2mdu	418	25	for	for	IN
cord-014597-66vd2mdu	418	26	a	a	DT
cord-014597-66vd2mdu	418	27	specific	specific	JJ
cord-014597-66vd2mdu	418	28	cell	cell	NN
cord-014597-66vd2mdu	418	29	line	line	NN
cord-014597-66vd2mdu	418	30	and	and	CC
cord-014597-66vd2mdu	418	31	medium	medium	NN
cord-014597-66vd2mdu	418	32	combination	combination	NN
cord-014597-66vd2mdu	418	33	,	,	,
cord-014597-66vd2mdu	418	34	and	and	CC
cord-014597-66vd2mdu	418	35	(	(	-LRB-
cord-014597-66vd2mdu	418	36	3	3	LS
cord-014597-66vd2mdu	418	37	)	)	-RRB-
cord-014597-66vd2mdu	418	38	Confirmation	confirmation	NN
cord-014597-66vd2mdu	418	39	of	of	IN
cord-014597-66vd2mdu	418	40	consistent	consistent	JJ
cord-014597-66vd2mdu	418	41	product	product	NN
cord-014597-66vd2mdu	418	42	quality	quality	NN
cord-014597-66vd2mdu	418	43	profiles	profile	NNS
cord-014597-66vd2mdu	418	44	between	between	IN
cord-014597-66vd2mdu	418	45	the	the	DT
cord-014597-66vd2mdu	418	46	small	small	JJ
cord-014597-66vd2mdu	418	47	scale	scale	NN
cord-014597-66vd2mdu	418	48	model	model	NN
cord-014597-66vd2mdu	418	49	and	and	CC
cord-014597-66vd2mdu	418	50	benchtop	benchtop	NN
cord-014597-66vd2mdu	418	51	perfusion	perfusion	NN
cord-014597-66vd2mdu	418	52	(	(	-LRB-
cord-014597-66vd2mdu	418	53	data	datum	NNS
cord-014597-66vd2mdu	418	54	not	not	RB
cord-014597-66vd2mdu	418	55	shown	show	VBN
cord-014597-66vd2mdu	418	56	)	)	-RRB-
cord-014597-66vd2mdu	418	57	.	.	.
cord-014597-66vd2mdu	419	1	A	a	DT
cord-014597-66vd2mdu	419	2	CHOZN	CHOZN	NNP
cord-014597-66vd2mdu	419	3	®	®	JJ
cord-014597-66vd2mdu	419	4	cell	cell	NN
cord-014597-66vd2mdu	419	5	line	line	NN
cord-014597-66vd2mdu	419	6	producing	produce	VBG
cord-014597-66vd2mdu	419	7	an	an	DT
cord-014597-66vd2mdu	419	8	IgG1	igg1	NN
cord-014597-66vd2mdu	419	9	was	be	VBD
cord-014597-66vd2mdu	419	10	evaluated	evaluate	VBN
cord-014597-66vd2mdu	419	11	in	in	IN
cord-014597-66vd2mdu	419	12	several	several	JJ
cord-014597-66vd2mdu	419	13	proprietary	proprietary	JJ
cord-014597-66vd2mdu	419	14	chemically	chemically	RB
cord-014597-66vd2mdu	419	15	defined	define	VBN
cord-014597-66vd2mdu	419	16	media	medium	NNS
cord-014597-66vd2mdu	419	17	prototypes	prototype	NNS
cord-014597-66vd2mdu	419	18	generated	generate	VBN
cord-014597-66vd2mdu	419	19	during	during	IN
cord-014597-66vd2mdu	419	20	the	the	DT
cord-014597-66vd2mdu	419	21	development	development	NN
cord-014597-66vd2mdu	419	22	of	of	IN
cord-014597-66vd2mdu	419	23	the	the	DT
cord-014597-66vd2mdu	419	24	catalog	catalog	NN
cord-014597-66vd2mdu	419	25	EXCELL	EXCELL	NNP
cord-014597-66vd2mdu	420	1	®	®	,
cord-014597-66vd2mdu	420	2	Advanced	advanced	JJ
cord-014597-66vd2mdu	420	3	HD	HD	NNP
cord-014597-66vd2mdu	420	4	Perfusion	Perfusion	NNP
cord-014597-66vd2mdu	420	5	medium	medium	NN
cord-014597-66vd2mdu	420	6	:	:	:
cord-014597-66vd2mdu	420	7	"	"	``
cord-014597-66vd2mdu	420	8	Fed	Fed	NNP
cord-014597-66vd2mdu	420	9	Batch	Batch	NNP
cord-014597-66vd2mdu	420	10	Medium	Medium	NNP
cord-014597-66vd2mdu	420	11	"	"	''
cord-014597-66vd2mdu	420	12	,	,	,
cord-014597-66vd2mdu	420	13	"	"	``
cord-014597-66vd2mdu	420	14	Early	Early	NNP
cord-014597-66vd2mdu	420	15	Prototype	Prototype	NNP
cord-014597-66vd2mdu	420	16	"	"	''
cord-014597-66vd2mdu	420	17	,	,	,
cord-014597-66vd2mdu	420	18	"	"	``
cord-014597-66vd2mdu	420	19	Mid	Mid	NNP
cord-014597-66vd2mdu	420	20	Prototype	Prototype	NNP
cord-014597-66vd2mdu	420	21	"	"	''
cord-014597-66vd2mdu	420	22	,	,	,
cord-014597-66vd2mdu	420	23	"	"	``
cord-014597-66vd2mdu	420	24	Intermediate	Intermediate	NNP
cord-014597-66vd2mdu	420	25	Prototype	Prototype	NNP
cord-014597-66vd2mdu	420	26	"	"	''
cord-014597-66vd2mdu	420	27	and	and	CC
cord-014597-66vd2mdu	420	28	"	"	``
cord-014597-66vd2mdu	420	29	Late	Late	NNP
cord-014597-66vd2mdu	420	30	Prototype	Prototype	NNP
cord-014597-66vd2mdu	420	31	"	"	''
cord-014597-66vd2mdu	420	32	[	[	-LRB-
cord-014597-66vd2mdu	420	33	2	2	CD
cord-014597-66vd2mdu	420	34	]	]	-RRB-
cord-014597-66vd2mdu	420	35	.	.	.
cord-014597-66vd2mdu	421	1	Small	small	JJ
cord-014597-66vd2mdu	421	2	scale	scale	NN
cord-014597-66vd2mdu	421	3	simulation	simulation	NN
cord-014597-66vd2mdu	421	4	of	of	IN
cord-014597-66vd2mdu	421	5	perfusion	perfusion	NN
cord-014597-66vd2mdu	421	6	experiments	experiment	NNS
cord-014597-66vd2mdu	421	7	were	be	VBD
cord-014597-66vd2mdu	421	8	run	run	VBN
cord-014597-66vd2mdu	421	9	in	in	IN
cord-014597-66vd2mdu	421	10	ambr	ambr	NNP
cord-014597-66vd2mdu	421	11	®	®	NNP
cord-014597-66vd2mdu	421	12	15	15	CD
cord-014597-66vd2mdu	421	13	.	.	.
cord-014597-66vd2mdu	422	1	Media	Media	NNP
cord-014597-66vd2mdu	422	2	exchange	exchange	NN
cord-014597-66vd2mdu	422	3	was	be	VBD
cord-014597-66vd2mdu	422	4	performed	perform	VBN
cord-014597-66vd2mdu	422	5	3	3	CD
cord-014597-66vd2mdu	422	6	times	time	NNS
cord-014597-66vd2mdu	422	7	per	per	IN
cord-014597-66vd2mdu	422	8	day	day	NN
cord-014597-66vd2mdu	422	9	in	in	IN
cord-014597-66vd2mdu	422	10	equal	equal	JJ
cord-014597-66vd2mdu	422	11	amounts	amount	NNS
cord-014597-66vd2mdu	422	12	.	.	.
cord-014597-66vd2mdu	423	1	Agitation	agitation	NN
cord-014597-66vd2mdu	423	2	,	,	,
cord-014597-66vd2mdu	423	3	gassing	gassing	NN
cord-014597-66vd2mdu	423	4	,	,	,
cord-014597-66vd2mdu	423	5	and	and	CC
cord-014597-66vd2mdu	423	6	liquid	liquid	JJ
cord-014597-66vd2mdu	423	7	handling	handling	NN
cord-014597-66vd2mdu	423	8	were	be	VBD
cord-014597-66vd2mdu	423	9	stopped	stop	VBN
cord-014597-66vd2mdu	423	10	for	for	IN
cord-014597-66vd2mdu	423	11	an	an	DT
cord-014597-66vd2mdu	423	12	optimized	optimized	JJ
cord-014597-66vd2mdu	423	13	period	period	NN
cord-014597-66vd2mdu	423	14	of	of	IN
cord-014597-66vd2mdu	423	15	time	time	NN
cord-014597-66vd2mdu	423	16	to	to	TO
cord-014597-66vd2mdu	423	17	allow	allow	VB
cord-014597-66vd2mdu	423	18	cells	cell	NNS
cord-014597-66vd2mdu	423	19	to	to	TO
cord-014597-66vd2mdu	423	20	settle	settle	VB
cord-014597-66vd2mdu	423	21	to	to	IN
cord-014597-66vd2mdu	423	22	the	the	DT
cord-014597-66vd2mdu	423	23	bottom	bottom	NN
cord-014597-66vd2mdu	423	24	.	.	.
cord-014597-66vd2mdu	424	1	Spent	spend	VBN
cord-014597-66vd2mdu	424	2	media	medium	NNS
cord-014597-66vd2mdu	424	3	was	be	VBD
cord-014597-66vd2mdu	424	4	removed	remove	VBN
cord-014597-66vd2mdu	424	5	in	in	IN
cord-014597-66vd2mdu	424	6	an	an	DT
cord-014597-66vd2mdu	424	7	amount	amount	NN
cord-014597-66vd2mdu	424	8	proportional	proportional	JJ
cord-014597-66vd2mdu	424	9	to	to	IN
cord-014597-66vd2mdu	424	10	1/3	1/3	DT
cord-014597-66vd2mdu	424	11	rd	rd	NNP
cord-014597-66vd2mdu	424	12	daily	daily	NNP
cord-014597-66vd2mdu	424	13	exchange	exchange	NN
cord-014597-66vd2mdu	424	14	volume	volume	NN
cord-014597-66vd2mdu	424	15	.	.	.
cord-014597-66vd2mdu	425	1	Agitation	agitation	NN
cord-014597-66vd2mdu	425	2	,	,	,
cord-014597-66vd2mdu	425	3	gassing	gassing	NN
cord-014597-66vd2mdu	425	4	,	,	,
cord-014597-66vd2mdu	425	5	and	and	CC
cord-014597-66vd2mdu	425	6	liquid	liquid	JJ
cord-014597-66vd2mdu	425	7	handling	handling	NN
cord-014597-66vd2mdu	425	8	were	be	VBD
cord-014597-66vd2mdu	425	9	resumed	resume	VBN
cord-014597-66vd2mdu	425	10	and	and	CC
cord-014597-66vd2mdu	425	11	fresh	fresh	JJ
cord-014597-66vd2mdu	425	12	media	medium	NNS
cord-014597-66vd2mdu	425	13	was	be	VBD
cord-014597-66vd2mdu	425	14	added	add	VBN
cord-014597-66vd2mdu	425	15	back	back	RB
cord-014597-66vd2mdu	425	16	to	to	IN
cord-014597-66vd2mdu	425	17	the	the	DT
cord-014597-66vd2mdu	425	18	vessels	vessel	NNS
cord-014597-66vd2mdu	425	19	.	.	.
cord-014597-66vd2mdu	426	1	For	for	IN
cord-014597-66vd2mdu	426	2	benchtop	benchtop	NN
cord-014597-66vd2mdu	426	3	perfusion	perfusion	NN
cord-014597-66vd2mdu	426	4	,	,	,
cord-014597-66vd2mdu	426	5	cells	cell	NNS
cord-014597-66vd2mdu	426	6	were	be	VBD
cord-014597-66vd2mdu	426	7	inoculated	inoculate	VBN
cord-014597-66vd2mdu	426	8	in	in	IN
cord-014597-66vd2mdu	426	9	3L	3l	CD
cord-014597-66vd2mdu	426	10	Applikon	Applikon	NNP
cord-014597-66vd2mdu	426	11	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	426	12	(	(	-LRB-
cord-014597-66vd2mdu	426	13	Applikon	Applikon	NNP
cord-014597-66vd2mdu	426	14	,	,	,
cord-014597-66vd2mdu	426	15	Netherlands	Netherlands	NNP
cord-014597-66vd2mdu	426	16	)	)	-RRB-
cord-014597-66vd2mdu	426	17	.	.	.
cord-014597-66vd2mdu	427	1	At	at	IN
cord-014597-66vd2mdu	427	2	a	a	DT
cord-014597-66vd2mdu	427	3	concentration	concentration	NN
cord-014597-66vd2mdu	427	4	of~6.0x10	of~6.0x10	NNP
cord-014597-66vd2mdu	427	5	6	6	CD
cord-014597-66vd2mdu	427	6	vc	vc	NNP
cord-014597-66vd2mdu	427	7	/	/	SYM
cord-014597-66vd2mdu	427	8	mL	ml	NN
cord-014597-66vd2mdu	427	9	,	,	,
cord-014597-66vd2mdu	427	10	perfusion	perfusion	NN
cord-014597-66vd2mdu	427	11	was	be	VBD
cord-014597-66vd2mdu	427	12	initiated	initiate	VBN
cord-014597-66vd2mdu	427	13	using	use	VBG
cord-014597-66vd2mdu	427	14	the	the	DT
cord-014597-66vd2mdu	427	15	ATF2	ATF2	NNP
cord-014597-66vd2mdu	427	16	(	(	-LRB-
cord-014597-66vd2mdu	427	17	RepliGen	RepliGen	NNP
cord-014597-66vd2mdu	427	18	,	,	,
cord-014597-66vd2mdu	427	19	Massachusetts	Massachusetts	NNP
cord-014597-66vd2mdu	427	20	)	)	-RRB-
cord-014597-66vd2mdu	427	21	.	.	.
cord-014597-66vd2mdu	428	1	Perfusion	perfusion	NN
cord-014597-66vd2mdu	428	2	rate	rate	NN
cord-014597-66vd2mdu	428	3	was	be	VBD
cord-014597-66vd2mdu	428	4	limited	limit	VBN
cord-014597-66vd2mdu	428	5	at	at	IN
cord-014597-66vd2mdu	428	6	1.2vvd	1.2vvd	CD
cord-014597-66vd2mdu	428	7	during	during	IN
cord-014597-66vd2mdu	428	8	steady	steady	JJ
cord-014597-66vd2mdu	428	9	-	-	HYPH
cord-014597-66vd2mdu	428	10	state	state	NN
cord-014597-66vd2mdu	428	11	.	.	.
cord-014597-66vd2mdu	429	1	Using	use	VBG
cord-014597-66vd2mdu	429	2	the	the	DT
cord-014597-66vd2mdu	429	3	cell	cell	NN
cord-014597-66vd2mdu	429	4	settling	settling	NN
cord-014597-66vd2mdu	429	5	method	method	NN
cord-014597-66vd2mdu	429	6	described	describe	VBN
cord-014597-66vd2mdu	429	7	above	above	IN
cord-014597-66vd2mdu	429	8	we	-PRON-	PRP
cord-014597-66vd2mdu	429	9	have	have	VBP
cord-014597-66vd2mdu	429	10	been	be	VBN
cord-014597-66vd2mdu	429	11	able	able	JJ
cord-014597-66vd2mdu	429	12	to	to	TO
cord-014597-66vd2mdu	429	13	achieve	achieve	VB
cord-014597-66vd2mdu	429	14	≥90	≥90	NNP
cord-014597-66vd2mdu	429	15	%	%	NN
cord-014597-66vd2mdu	429	16	cell	cell	NN
cord-014597-66vd2mdu	429	17	retention	retention	NN
cord-014597-66vd2mdu	429	18	efficiency	efficiency	NN
cord-014597-66vd2mdu	429	19	.	.	.
cord-014597-66vd2mdu	430	1	All	all	DT
cord-014597-66vd2mdu	430	2	media	medium	NNS
cord-014597-66vd2mdu	430	3	tested	test	VBN
cord-014597-66vd2mdu	430	4	in	in	IN
cord-014597-66vd2mdu	430	5	this	this	DT
cord-014597-66vd2mdu	430	6	work	work	NN
cord-014597-66vd2mdu	430	7	were	be	VBD
cord-014597-66vd2mdu	430	8	able	able	JJ
cord-014597-66vd2mdu	430	9	to	to	TO
cord-014597-66vd2mdu	430	10	reach	reach	VB
cord-014597-66vd2mdu	430	11	and	and	CC
cord-014597-66vd2mdu	430	12	maintain	maintain	VB
cord-014597-66vd2mdu	430	13	the	the	DT
cord-014597-66vd2mdu	430	14	30x10	30x10	CD
cord-014597-66vd2mdu	430	15	6	6	CD
cord-014597-66vd2mdu	430	16	vc	vc	NNP
cord-014597-66vd2mdu	430	17	/	/	SYM
cord-014597-66vd2mdu	430	18	mL	ml	NN
cord-014597-66vd2mdu	430	19	target	target	NN
cord-014597-66vd2mdu	430	20	cell	cell	NN
cord-014597-66vd2mdu	430	21	density	density	NN
cord-014597-66vd2mdu	430	22	at	at	IN
cord-014597-66vd2mdu	430	23	1vvd	1vvd	CD
cord-014597-66vd2mdu	431	1	(	(	-LRB-
cord-014597-66vd2mdu	431	2	Fig	Fig	NNP
cord-014597-66vd2mdu	431	3	.	.	NNP
cord-014597-66vd2mdu	431	4	1	1	CD
cord-014597-66vd2mdu	431	5	)	)	-RRB-
cord-014597-66vd2mdu	431	6	.	.	.
cord-014597-66vd2mdu	432	1	Performance	performance	NN
cord-014597-66vd2mdu	432	2	of	of	IN
cord-014597-66vd2mdu	432	3	each	each	DT
cord-014597-66vd2mdu	432	4	media	medium	NNS
cord-014597-66vd2mdu	432	5	formulation	formulation	NN
cord-014597-66vd2mdu	432	6	was	be	VBD
cord-014597-66vd2mdu	432	7	ranked	rank	VBN
cord-014597-66vd2mdu	432	8	based	base	VBN
cord-014597-66vd2mdu	432	9	on	on	IN
cord-014597-66vd2mdu	432	10	specific	specific	JJ
cord-014597-66vd2mdu	432	11	productivity	productivity	NN
cord-014597-66vd2mdu	432	12	(	(	-LRB-
cord-014597-66vd2mdu	432	13	Table	table	NN
cord-014597-66vd2mdu	432	14	1	1	CD
cord-014597-66vd2mdu	432	15	)	)	-RRB-
cord-014597-66vd2mdu	432	16	.	.	.
cord-014597-66vd2mdu	433	1	Using	use	VBG
cord-014597-66vd2mdu	433	2	"	"	``
cord-014597-66vd2mdu	433	3	Intermediate	Intermediate	NNP
cord-014597-66vd2mdu	433	4	Prototype	Prototype	NNP
cord-014597-66vd2mdu	433	5	"	"	''
cord-014597-66vd2mdu	433	6	,	,	,
cord-014597-66vd2mdu	433	7	minimum	minimum	JJ
cord-014597-66vd2mdu	433	8	steady	steady	JJ
cord-014597-66vd2mdu	433	9	-	-	HYPH
cord-014597-66vd2mdu	433	10	state	state	NN
cord-014597-66vd2mdu	433	11	CSPR	CSPR	NNP
cord-014597-66vd2mdu	433	12	was	be	VBD
cord-014597-66vd2mdu	433	13	determined	determine	VBN
cord-014597-66vd2mdu	433	14	to	to	TO
cord-014597-66vd2mdu	433	15	be	be	VB
cord-014597-66vd2mdu	433	16	33.3pL	33.3pl	CD
cord-014597-66vd2mdu	433	17	/	/	SYM
cord-014597-66vd2mdu	433	18	c	c	NN
cord-014597-66vd2mdu	433	19	/	/	SYM
cord-014597-66vd2mdu	433	20	d	d	NN
cord-014597-66vd2mdu	433	21	for	for	IN
cord-014597-66vd2mdu	433	22	this	this	DT
cord-014597-66vd2mdu	433	23	cell	cell	NN
cord-014597-66vd2mdu	433	24	line	line	NN
cord-014597-66vd2mdu	433	25	.	.	.
cord-014597-66vd2mdu	434	1	N	n	CD
cord-014597-66vd2mdu	434	2	-	-	HYPH
cord-014597-66vd2mdu	434	3	glycan	glycan	NN
cord-014597-66vd2mdu	434	4	analysis	analysis	NN
cord-014597-66vd2mdu	434	5	of	of	IN
cord-014597-66vd2mdu	434	6	ambr	ambr	NNP
cord-014597-66vd2mdu	434	7	®	®	NNP
cord-014597-66vd2mdu	434	8	15	15	CD
cord-014597-66vd2mdu	434	9	and	and	CC
cord-014597-66vd2mdu	434	10	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	434	11	samples	sample	NNS
cord-014597-66vd2mdu	434	12	via	via	IN
cord-014597-66vd2mdu	434	13	intact	intact	JJ
cord-014597-66vd2mdu	434	14	mass	mass	NN
cord-014597-66vd2mdu	434	15	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	434	16	displayed	display	VBN
cord-014597-66vd2mdu	434	17	only	only	RB
cord-014597-66vd2mdu	434	18	slight	slight	JJ
cord-014597-66vd2mdu	434	19	differences	difference	NNS
cord-014597-66vd2mdu	434	20	in	in	IN
cord-014597-66vd2mdu	434	21	product	product	NN
cord-014597-66vd2mdu	434	22	quality	quality	NN
cord-014597-66vd2mdu	434	23	profile	profile	NN
cord-014597-66vd2mdu	434	24	(	(	-LRB-
cord-014597-66vd2mdu	434	25	data	datum	NNS
cord-014597-66vd2mdu	434	26	not	not	RB
cord-014597-66vd2mdu	434	27	shown	show	VBN
cord-014597-66vd2mdu	434	28	)	)	-RRB-
cord-014597-66vd2mdu	434	29	.	.	.
cord-014597-66vd2mdu	435	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	435	2	work	work	NN
cord-014597-66vd2mdu	435	3	has	have	VBZ
cord-014597-66vd2mdu	435	4	shown	show	VBN
cord-014597-66vd2mdu	435	5	a	a	DT
cord-014597-66vd2mdu	435	6	clear	clear	JJ
cord-014597-66vd2mdu	435	7	distinction	distinction	NN
cord-014597-66vd2mdu	435	8	between	between	IN
cord-014597-66vd2mdu	435	9	various	various	JJ
cord-014597-66vd2mdu	435	10	prototype	prototype	NN
cord-014597-66vd2mdu	435	11	perfusion	perfusion	NN
cord-014597-66vd2mdu	435	12	media	medium	NNS
cord-014597-66vd2mdu	435	13	and	and	CC
cord-014597-66vd2mdu	435	14	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	435	15	a	a	DT
cord-014597-66vd2mdu	435	16	50	50	CD
cord-014597-66vd2mdu	435	17	%	%	NN
cord-014597-66vd2mdu	435	18	increase	increase	NN
cord-014597-66vd2mdu	435	19	in	in	IN
cord-014597-66vd2mdu	435	20	specific	specific	JJ
cord-014597-66vd2mdu	435	21	productivity	productivity	NN
cord-014597-66vd2mdu	435	22	over	over	IN
cord-014597-66vd2mdu	435	23	"	"	``
cord-014597-66vd2mdu	435	24	Fed	Fed	NNP
cord-014597-66vd2mdu	435	25	Batch	Batch	NNP
cord-014597-66vd2mdu	435	26	Medium	Medium	NNP
cord-014597-66vd2mdu	435	27	"	"	''
cord-014597-66vd2mdu	435	28	used	use	VBN
cord-014597-66vd2mdu	435	29	in	in	IN
cord-014597-66vd2mdu	435	30	perfusion	perfusion	NN
cord-014597-66vd2mdu	435	31	.	.	.
cord-014597-66vd2mdu	436	1	Additionally	additionally	RB
cord-014597-66vd2mdu	436	2	,	,	,
cord-014597-66vd2mdu	436	3	we	-PRON-	PRP
cord-014597-66vd2mdu	436	4	have	have	VBP
cord-014597-66vd2mdu	436	5	shown	show	VBN
cord-014597-66vd2mdu	436	6	the	the	DT
cord-014597-66vd2mdu	436	7	application	application	NN
cord-014597-66vd2mdu	436	8	to	to	TO
cord-014597-66vd2mdu	436	9	further	further	RB
cord-014597-66vd2mdu	436	10	characterize	characterize	VB
cord-014597-66vd2mdu	436	11	the	the	DT
cord-014597-66vd2mdu	436	12	process	process	NN
cord-014597-66vd2mdu	436	13	using	use	VBG
cord-014597-66vd2mdu	436	14	this	this	DT
cord-014597-66vd2mdu	436	15	model	model	NN
cord-014597-66vd2mdu	436	16	to	to	TO
cord-014597-66vd2mdu	436	17	determine	determine	VB
cord-014597-66vd2mdu	436	18	CSPR	CSPR	NNP
cord-014597-66vd2mdu	436	19	min	min	NNP
cord-014597-66vd2mdu	436	20	for	for	IN
cord-014597-66vd2mdu	436	21	a	a	DT
cord-014597-66vd2mdu	436	22	given	give	VBN
cord-014597-66vd2mdu	436	23	medium	medium	NN
cord-014597-66vd2mdu	436	24	and	and	CC
cord-014597-66vd2mdu	436	25	cell	cell	NN
cord-014597-66vd2mdu	436	26	line	line	NN
cord-014597-66vd2mdu	436	27	.	.	.
cord-014597-66vd2mdu	437	1	The	the	DT
cord-014597-66vd2mdu	437	2	transient	transient	JJ
cord-014597-66vd2mdu	437	3	process	process	NN
cord-014597-66vd2mdu	437	4	has	have	VBZ
cord-014597-66vd2mdu	437	5	been	be	VBN
cord-014597-66vd2mdu	437	6	successfully	successfully	RB
cord-014597-66vd2mdu	437	7	operated	operate	VBN
cord-014597-66vd2mdu	437	8	at	at	IN
cord-014597-66vd2mdu	437	9	500L	500l	CD
cord-014597-66vd2mdu	437	10	in	in	IN
cord-014597-66vd2mdu	437	11	a	a	DT
cord-014597-66vd2mdu	437	12	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	437	13	Biostat	Biostat	NNP
cord-014597-66vd2mdu	437	14	Single	single	JJ
cord-014597-66vd2mdu	437	15	Use	Use	NNP
cord-014597-66vd2mdu	437	16	Bioreactor	Bioreactor	NNP
cord-014597-66vd2mdu	437	17	(	(	-LRB-
cord-014597-66vd2mdu	437	18	SUB	SUB	NNP
cord-014597-66vd2mdu	437	19	)	)	-RRB-
cord-014597-66vd2mdu	437	20	,	,	,
cord-014597-66vd2mdu	437	21	yielding	yield	VBG
cord-014597-66vd2mdu	437	22	0.4	0.4	CD
cord-014597-66vd2mdu	437	23	kg	kg	NNS
cord-014597-66vd2mdu	437	24	of	of	IN
cord-014597-66vd2mdu	437	25	crude	crude	JJ
cord-014597-66vd2mdu	437	26	product	product	NN
cord-014597-66vd2mdu	437	27	from	from	IN
cord-014597-66vd2mdu	437	28	a	a	DT
cord-014597-66vd2mdu	437	29	two	two	CD
cord-014597-66vd2mdu	437	30	-	-	HYPH
cord-014597-66vd2mdu	437	31	week	week	NN
cord-014597-66vd2mdu	437	32	expression	expression	NN
cord-014597-66vd2mdu	437	33	culture	culture	NN
cord-014597-66vd2mdu	437	34	(	(	-LRB-
cord-014597-66vd2mdu	437	35	Table	table	NN
cord-014597-66vd2mdu	437	36	1	1	CD
cord-014597-66vd2mdu	437	37	)	)	-RRB-
cord-014597-66vd2mdu	437	38	.	.	.
cord-014597-66vd2mdu	438	1	Successful	successful	JJ
cord-014597-66vd2mdu	438	2	scale	scale	NN
cord-014597-66vd2mdu	438	3	up	up	RP
cord-014597-66vd2mdu	438	4	of	of	IN
cord-014597-66vd2mdu	438	5	the	the	DT
cord-014597-66vd2mdu	438	6	process	process	NN
cord-014597-66vd2mdu	438	7	to	to	IN
cord-014597-66vd2mdu	438	8	500L	500L	NNP
cord-014597-66vd2mdu	438	9	creates	create	VBZ
cord-014597-66vd2mdu	438	10	the	the	DT
cord-014597-66vd2mdu	438	11	potential	potential	NN
cord-014597-66vd2mdu	438	12	to	to	TO
cord-014597-66vd2mdu	438	13	supply	supply	VB
cord-014597-66vd2mdu	438	14	transiently	transiently	RB
cord-014597-66vd2mdu	438	15	expressed	express	VBN
cord-014597-66vd2mdu	438	16	products	product	NNS
cord-014597-66vd2mdu	438	17	to	to	TO
cord-014597-66vd2mdu	438	18	support	support	VB
cord-014597-66vd2mdu	438	19	toxicology	toxicology	NN
cord-014597-66vd2mdu	438	20	studies	study	NNS
cord-014597-66vd2mdu	438	21	or	or	CC
cord-014597-66vd2mdu	438	22	even	even	RB
cord-014597-66vd2mdu	438	23	early	early	JJ
cord-014597-66vd2mdu	438	24	GMP	GMP	NNP
cord-014597-66vd2mdu	438	25	clinical	clinical	JJ
cord-014597-66vd2mdu	438	26	supply	supply	NN
cord-014597-66vd2mdu	438	27	,	,	,
cord-014597-66vd2mdu	438	28	enabling	enable	VBG
cord-014597-66vd2mdu	438	29	accelerated	accelerate	VBN
cord-014597-66vd2mdu	438	30	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	438	31	development	development	NN
cord-014597-66vd2mdu	438	32	project	project	NN
cord-014597-66vd2mdu	438	33	timelines	timeline	NNS
cord-014597-66vd2mdu	438	34	.	.	.
cord-014597-66vd2mdu	439	1	The	the	DT
cord-014597-66vd2mdu	439	2	scale	scale	NN
cord-014597-66vd2mdu	439	3	up	up	RP
cord-014597-66vd2mdu	439	4	from	from	IN
cord-014597-66vd2mdu	439	5	rocking	rock	VBG
cord-014597-66vd2mdu	439	6	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	439	7	(	(	-LRB-
cord-014597-66vd2mdu	439	8	RBr	rbr	NN
cord-014597-66vd2mdu	439	9	)	)	-RRB-
cord-014597-66vd2mdu	439	10	to	to	TO
cord-014597-66vd2mdu	439	11	SUB	sub	VB
cord-014597-66vd2mdu	439	12	scale	scale	NN
cord-014597-66vd2mdu	439	13	identified	identify	VBD
cord-014597-66vd2mdu	439	14	some	some	DT
cord-014597-66vd2mdu	439	15	scalability	scalability	NN
cord-014597-66vd2mdu	439	16	issues	issue	NNS
cord-014597-66vd2mdu	439	17	.	.	.
cord-014597-66vd2mdu	440	1	Lower	lower	VB
cord-014597-66vd2mdu	440	2	specific	specific	JJ
cord-014597-66vd2mdu	440	3	productivity	productivity	NN
cord-014597-66vd2mdu	440	4	due	due	JJ
cord-014597-66vd2mdu	440	5	to	to	IN
cord-014597-66vd2mdu	440	6	increased	increase	VBN
cord-014597-66vd2mdu	440	7	cell	cell	NN
cord-014597-66vd2mdu	440	8	growth	growth	NN
cord-014597-66vd2mdu	440	9	and	and	CC
cord-014597-66vd2mdu	440	10	decreased	decrease	VBD
cord-014597-66vd2mdu	440	11	titres	titre	NNS
cord-014597-66vd2mdu	440	12	were	be	VBD
cord-014597-66vd2mdu	440	13	observed	observe	VBN
cord-014597-66vd2mdu	440	14	in	in	IN
cord-014597-66vd2mdu	440	15	the	the	DT
cord-014597-66vd2mdu	440	16	SUB	sub	NN
cord-014597-66vd2mdu	441	1	(	(	-LRB-
cord-014597-66vd2mdu	441	2	Fig	Fig	NNP
cord-014597-66vd2mdu	441	3	.	.	NNP
cord-014597-66vd2mdu	441	4	1	1	CD
cord-014597-66vd2mdu	441	5	iii	iii	NNP
cord-014597-66vd2mdu	441	6	&	&	CC
cord-014597-66vd2mdu	441	7	iv	iv	NNP
cord-014597-66vd2mdu	441	8	)	)	-RRB-
cord-014597-66vd2mdu	441	9	.	.	.
cord-014597-66vd2mdu	442	1	To	to	TO
cord-014597-66vd2mdu	442	2	improve	improve	VB
cord-014597-66vd2mdu	442	3	the	the	DT
cord-014597-66vd2mdu	442	4	predictability	predictability	NN
cord-014597-66vd2mdu	442	5	of	of	IN
cord-014597-66vd2mdu	442	6	scale	scale	NN
cord-014597-66vd2mdu	442	7	up	up	RP
cord-014597-66vd2mdu	442	8	,	,	,
cord-014597-66vd2mdu	442	9	a	a	DT
cord-014597-66vd2mdu	442	10	new	new	JJ
cord-014597-66vd2mdu	442	11	process	process	NN
cord-014597-66vd2mdu	442	12	was	be	VBD
cord-014597-66vd2mdu	442	13	developed	develop	VBN
cord-014597-66vd2mdu	442	14	and	and	CC
cord-014597-66vd2mdu	442	15	evaluated	evaluate	VBN
cord-014597-66vd2mdu	442	16	in	in	IN
cord-014597-66vd2mdu	442	17	the	the	DT
cord-014597-66vd2mdu	442	18	SUB	sub	NN
cord-014597-66vd2mdu	442	19	vessels	vessel	NNS
cord-014597-66vd2mdu	442	20	utilising	utilise	VBG
cord-014597-66vd2mdu	442	21	a	a	DT
cord-014597-66vd2mdu	442	22	modified	modify	VBN
cord-014597-66vd2mdu	442	23	transfection	transfection	NN
cord-014597-66vd2mdu	442	24	method	method	NN
cord-014597-66vd2mdu	442	25	,	,	,
cord-014597-66vd2mdu	442	26	which	which	WDT
cord-014597-66vd2mdu	442	27	resulted	result	VBD
cord-014597-66vd2mdu	442	28	in	in	IN
cord-014597-66vd2mdu	442	29	comparable	comparable	JJ
cord-014597-66vd2mdu	442	30	expression	expression	NN
cord-014597-66vd2mdu	442	31	levels	level	NNS
cord-014597-66vd2mdu	442	32	and	and	CC
cord-014597-66vd2mdu	442	33	specific	specific	JJ
cord-014597-66vd2mdu	442	34	productivity	productivity	NN
cord-014597-66vd2mdu	442	35	between	between	IN
cord-014597-66vd2mdu	442	36	RBr	rbr	NN
cord-014597-66vd2mdu	442	37	and	and	CC
cord-014597-66vd2mdu	442	38	SUB	sub	NN
cord-014597-66vd2mdu	442	39	scales	scale	NNS
cord-014597-66vd2mdu	442	40	.	.	.
cord-014597-66vd2mdu	443	1	Two	two	CD
cord-014597-66vd2mdu	443	2	sets	set	NNS
cord-014597-66vd2mdu	443	3	of	of	IN
cord-014597-66vd2mdu	443	4	expression	expression	NN
cord-014597-66vd2mdu	443	5	vectors	vector	NNS
cord-014597-66vd2mdu	443	6	comprising	comprise	VBG
cord-014597-66vd2mdu	443	7	heavy	heavy	JJ
cord-014597-66vd2mdu	443	8	chain	chain	NN
cord-014597-66vd2mdu	443	9	and	and	CC
cord-014597-66vd2mdu	443	10	light	light	NN
cord-014597-66vd2mdu	443	11	chain	chain	NN
cord-014597-66vd2mdu	443	12	plasmids	plasmid	NNS
cord-014597-66vd2mdu	443	13	expressing	express	VBG
cord-014597-66vd2mdu	443	14	a	a	DT
cord-014597-66vd2mdu	443	15	human	human	JJ
cord-014597-66vd2mdu	443	16	IgG1	IgG1	NNP
cord-014597-66vd2mdu	443	17	kappa	kappa	NN
cord-014597-66vd2mdu	443	18	mAb	mAb	NNP
cord-014597-66vd2mdu	443	19	,	,	,
cord-014597-66vd2mdu	443	20	as	as	IN
cord-014597-66vd2mdu	443	21	previously	previously	RB
cord-014597-66vd2mdu	443	22	described	describe	VBN
cord-014597-66vd2mdu	443	23	[	[	-LRB-
cord-014597-66vd2mdu	443	24	1	1	CD
cord-014597-66vd2mdu	443	25	,	,	,
cord-014597-66vd2mdu	443	26	2	2	CD
cord-014597-66vd2mdu	443	27	]	]	-RRB-
cord-014597-66vd2mdu	443	28	were	be	VBD
cord-014597-66vd2mdu	443	29	used	use	VBN
cord-014597-66vd2mdu	443	30	in	in	IN
cord-014597-66vd2mdu	443	31	the	the	DT
cord-014597-66vd2mdu	443	32	process	process	NN
cord-014597-66vd2mdu	443	33	optimisation	optimisation	NN
cord-014597-66vd2mdu	443	34	study	study	NN
cord-014597-66vd2mdu	443	35	.	.	.
cord-014597-66vd2mdu	444	1	The	the	DT
cord-014597-66vd2mdu	444	2	cell	cell	NN
cord-014597-66vd2mdu	444	3	line	line	NN
cord-014597-66vd2mdu	444	4	used	use	VBN
cord-014597-66vd2mdu	444	5	for	for	IN
cord-014597-66vd2mdu	444	6	transient	transient	JJ
cord-014597-66vd2mdu	444	7	expression	expression	NN
cord-014597-66vd2mdu	444	8	and	and	CC
cord-014597-66vd2mdu	444	9	the	the	DT
cord-014597-66vd2mdu	444	10	PEI	PEI	NNP
cord-014597-66vd2mdu	444	11	mediated	mediate	VBD
cord-014597-66vd2mdu	444	12	transfection	transfection	NN
cord-014597-66vd2mdu	444	13	method	method	NN
cord-014597-66vd2mdu	444	14	has	have	VBZ
cord-014597-66vd2mdu	444	15	been	be	VBN
cord-014597-66vd2mdu	444	16	described	describe	VBN
cord-014597-66vd2mdu	444	17	previously	previously	RB
cord-014597-66vd2mdu	444	18	[	[	-LRB-
cord-014597-66vd2mdu	444	19	1	1	CD
cord-014597-66vd2mdu	444	20	]	]	-RRB-
cord-014597-66vd2mdu	444	21	.	.	.
cord-014597-66vd2mdu	445	1	Transfected	transfecte	VBN
cord-014597-66vd2mdu	445	2	cultures	culture	NNS
cord-014597-66vd2mdu	445	3	were	be	VBD
cord-014597-66vd2mdu	445	4	run	run	VBN
cord-014597-66vd2mdu	445	5	under	under	IN
cord-014597-66vd2mdu	445	6	fed	fed	NNP
cord-014597-66vd2mdu	445	7	batch	batch	NN
cord-014597-66vd2mdu	445	8	conditions	condition	NNS
cord-014597-66vd2mdu	445	9	for	for	IN
cord-014597-66vd2mdu	445	10	14	14	CD
cord-014597-66vd2mdu	445	11	days	day	NNS
cord-014597-66vd2mdu	445	12	in	in	IN
cord-014597-66vd2mdu	445	13	22L	22L	NNP
cord-014597-66vd2mdu	445	14	GE	GE	NNP
cord-014597-66vd2mdu	445	15	Healthcare	Healthcare	NNP
cord-014597-66vd2mdu	445	16	Wave	Wave	NNP
cord-014597-66vd2mdu	445	17	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	445	18	(	(	-LRB-
cord-014597-66vd2mdu	445	19	RBr	RBr	NNP
cord-014597-66vd2mdu	445	20	)	)	-RRB-
cord-014597-66vd2mdu	445	21	,	,	,
cord-014597-66vd2mdu	445	22	Hyclone	Hyclone	NNP
cord-014597-66vd2mdu	445	23	SUB	SUB	NNP
cord-014597-66vd2mdu	445	24	using	use	VBG
cord-014597-66vd2mdu	445	25	50L	50l	CD
cord-014597-66vd2mdu	445	26	and	and	CC
cord-014597-66vd2mdu	445	27	250L	250l	CD
cord-014597-66vd2mdu	445	28	Hyclone	Hyclone	NNP
cord-014597-66vd2mdu	445	29	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	445	30	bags	bag	NNS
cord-014597-66vd2mdu	445	31	(	(	-LRB-
cord-014597-66vd2mdu	445	32	Thermo	Thermo	NNP
cord-014597-66vd2mdu	445	33	Scientific	Scientific	NNP
cord-014597-66vd2mdu	445	34	)	)	-RRB-
cord-014597-66vd2mdu	445	35	.	.	.
cord-014597-66vd2mdu	446	1	The	the	DT
cord-014597-66vd2mdu	446	2	transfection	transfection	NN
cord-014597-66vd2mdu	446	3	process	process	NN
cord-014597-66vd2mdu	446	4	was	be	VBD
cord-014597-66vd2mdu	446	5	modified	modify	VBN
cord-014597-66vd2mdu	446	6	to	to	TO
cord-014597-66vd2mdu	446	7	address	address	VB
cord-014597-66vd2mdu	446	8	the	the	DT
cord-014597-66vd2mdu	446	9	reduced	reduced	JJ
cord-014597-66vd2mdu	446	10	titres	titre	NNS
cord-014597-66vd2mdu	446	11	and	and	CC
cord-014597-66vd2mdu	446	12	higher	high	JJR
cord-014597-66vd2mdu	446	13	viable	viable	JJ
cord-014597-66vd2mdu	446	14	cell	cell	NN
cord-014597-66vd2mdu	446	15	density	density	NN
cord-014597-66vd2mdu	446	16	(	(	-LRB-
cord-014597-66vd2mdu	446	17	VCD	vcd	NN
cord-014597-66vd2mdu	446	18	)	)	-RRB-
cord-014597-66vd2mdu	446	19	seen	see	VBN
cord-014597-66vd2mdu	446	20	in	in	IN
cord-014597-66vd2mdu	446	21	the	the	DT
cord-014597-66vd2mdu	446	22	SUB	sub	NN
cord-014597-66vd2mdu	446	23	cultures	culture	NNS
cord-014597-66vd2mdu	446	24	.	.	.
cord-014597-66vd2mdu	447	1	Shake	shake	VB
cord-014597-66vd2mdu	447	2	flask	flask	NN
cord-014597-66vd2mdu	447	3	cultures	culture	NNS
cord-014597-66vd2mdu	447	4	were	be	VBD
cord-014597-66vd2mdu	447	5	used	use	VBN
cord-014597-66vd2mdu	447	6	to	to	TO
cord-014597-66vd2mdu	447	7	assess	assess	VB
cord-014597-66vd2mdu	447	8	the	the	DT
cord-014597-66vd2mdu	447	9	standard	standard	NN
cord-014597-66vd2mdu	447	10	(	(	-LRB-
cord-014597-66vd2mdu	447	11	A	a	NN
cord-014597-66vd2mdu	447	12	)	)	-RRB-
cord-014597-66vd2mdu	447	13	and	and	CC
cord-014597-66vd2mdu	447	14	modified	modify	VBN
cord-014597-66vd2mdu	447	15	transfection	transfection	NN
cord-014597-66vd2mdu	447	16	processes	process	NNS
cord-014597-66vd2mdu	447	17	(	(	-LRB-
cord-014597-66vd2mdu	447	18	B	b	NN
cord-014597-66vd2mdu	447	19	and	and	CC
cord-014597-66vd2mdu	447	20	C	C	NNP
cord-014597-66vd2mdu	447	21	)	)	-RRB-
cord-014597-66vd2mdu	447	22	(	(	-LRB-
cord-014597-66vd2mdu	447	23	Fig	fig	NN
cord-014597-66vd2mdu	447	24	.	.	.
cord-014597-66vd2mdu	448	1	1	1	LS
cord-014597-66vd2mdu	448	2	,	,	,
cord-014597-66vd2mdu	448	3	i	i	NNP
cord-014597-66vd2mdu	448	4	&	&	CC
cord-014597-66vd2mdu	448	5	ii	ii	NNP
cord-014597-66vd2mdu	448	6	)	)	-RRB-
cord-014597-66vd2mdu	448	7	.	.	.
cord-014597-66vd2mdu	449	1	Process	process	NN
cord-014597-66vd2mdu	449	2	C	C	NNP
cord-014597-66vd2mdu	449	3	was	be	VBD
cord-014597-66vd2mdu	449	4	identified	identify	VBN
cord-014597-66vd2mdu	449	5	as	as	IN
cord-014597-66vd2mdu	449	6	the	the	DT
cord-014597-66vd2mdu	449	7	process	process	NN
cord-014597-66vd2mdu	449	8	to	to	TO
cord-014597-66vd2mdu	449	9	be	be	VB
cord-014597-66vd2mdu	449	10	studied	study	VBN
cord-014597-66vd2mdu	449	11	at	at	IN
cord-014597-66vd2mdu	449	12	SUB	sub	NN
cord-014597-66vd2mdu	449	13	scale	scale	NN
cord-014597-66vd2mdu	449	14	,	,	,
cord-014597-66vd2mdu	449	15	offering	offer	VBG
cord-014597-66vd2mdu	449	16	the	the	DT
cord-014597-66vd2mdu	449	17	potential	potential	NN
cord-014597-66vd2mdu	449	18	to	to	TO
cord-014597-66vd2mdu	449	19	mitigate	mitigate	VB
cord-014597-66vd2mdu	449	20	the	the	DT
cord-014597-66vd2mdu	449	21	high	high	JJ
cord-014597-66vd2mdu	449	22	viable	viable	JJ
cord-014597-66vd2mdu	449	23	cell	cell	NN
cord-014597-66vd2mdu	449	24	densities	density	NNS
cord-014597-66vd2mdu	449	25	(	(	-LRB-
cord-014597-66vd2mdu	449	26	VCD	VCD	NNP
cord-014597-66vd2mdu	449	27	)	)	-RRB-
cord-014597-66vd2mdu	449	28	observed	observe	VBD
cord-014597-66vd2mdu	449	29	.	.	.
cord-014597-66vd2mdu	450	1	Scaling	scale	VBG
cord-014597-66vd2mdu	450	2	up	up	RP
cord-014597-66vd2mdu	450	3	process	process	NN
cord-014597-66vd2mdu	450	4	C	c	NN
cord-014597-66vd2mdu	450	5	to	to	IN
cord-014597-66vd2mdu	450	6	50L	50l	CD
cord-014597-66vd2mdu	450	7	and	and	CC
cord-014597-66vd2mdu	450	8	250L	250l	CD
cord-014597-66vd2mdu	451	1	SUB	SUB	NNP
cord-014597-66vd2mdu	451	2	resulted	result	VBD
cord-014597-66vd2mdu	451	3	in	in	IN
cord-014597-66vd2mdu	451	4	cultures	culture	NNS
cord-014597-66vd2mdu	451	5	producing	produce	VBG
cord-014597-66vd2mdu	451	6	titres	titre	NNS
cord-014597-66vd2mdu	451	7	exceeding	exceed	VBG
cord-014597-66vd2mdu	451	8	1g	1g	CD
cord-014597-66vd2mdu	451	9	/	/	SYM
cord-014597-66vd2mdu	451	10	L	l	NN
cord-014597-66vd2mdu	451	11	with	with	IN
cord-014597-66vd2mdu	451	12	desired	desire	VBN
cord-014597-66vd2mdu	451	13	cell	cell	NN
cord-014597-66vd2mdu	451	14	growth	growth	NN
cord-014597-66vd2mdu	451	15	profiles	profile	NNS
cord-014597-66vd2mdu	451	16	.	.	.
cord-014597-66vd2mdu	452	1	Scale	scale	VB
cord-014597-66vd2mdu	452	2	up	up	RP
cord-014597-66vd2mdu	452	3	of	of	IN
cord-014597-66vd2mdu	452	4	process	process	NN
cord-014597-66vd2mdu	452	5	A	a	NN
cord-014597-66vd2mdu	452	6	into	into	IN
cord-014597-66vd2mdu	452	7	SUB	sub	NN
cord-014597-66vd2mdu	452	8	vessels	vessel	NNS
cord-014597-66vd2mdu	452	9	resulted	result	VBN
cord-014597-66vd2mdu	452	10	in	in	IN
cord-014597-66vd2mdu	452	11	decreased	decreased	JJ
cord-014597-66vd2mdu	452	12	productivity	productivity	NN
cord-014597-66vd2mdu	452	13	compared	compare	VBN
cord-014597-66vd2mdu	452	14	to	to	IN
cord-014597-66vd2mdu	452	15	the	the	DT
cord-014597-66vd2mdu	452	16	RBr	rbr	NN
cord-014597-66vd2mdu	452	17	scale	scale	NN
cord-014597-66vd2mdu	452	18	.	.	.
cord-014597-66vd2mdu	453	1	After	after	IN
cord-014597-66vd2mdu	453	2	optimisation	optimisation	NN
cord-014597-66vd2mdu	453	3	,	,	,
cord-014597-66vd2mdu	453	4	the	the	DT
cord-014597-66vd2mdu	453	5	SUB	sub	NN
cord-014597-66vd2mdu	453	6	process	process	NN
cord-014597-66vd2mdu	453	7	C	c	NN
cord-014597-66vd2mdu	453	8	yielded	yield	VBD
cord-014597-66vd2mdu	453	9	increased	increase	VBN
cord-014597-66vd2mdu	453	10	specific	specific	JJ
cord-014597-66vd2mdu	453	11	productivities	productivity	NNS
cord-014597-66vd2mdu	453	12	and	and	CC
cord-014597-66vd2mdu	453	13	expression	expression	NN
cord-014597-66vd2mdu	453	14	titres	titre	NNS
cord-014597-66vd2mdu	453	15	comparable	comparable	JJ
cord-014597-66vd2mdu	453	16	to	to	IN
cord-014597-66vd2mdu	453	17	those	those	DT
cord-014597-66vd2mdu	453	18	seen	see	VBN
cord-014597-66vd2mdu	453	19	at	at	IN
cord-014597-66vd2mdu	453	20	RBr	rbr	NN
cord-014597-66vd2mdu	453	21	scale	scale	NN
cord-014597-66vd2mdu	453	22	(	(	-LRB-
cord-014597-66vd2mdu	453	23	Table	table	NN
cord-014597-66vd2mdu	453	24	1	1	CD
cord-014597-66vd2mdu	453	25	)	)	-RRB-
cord-014597-66vd2mdu	453	26	.	.	.
cord-014597-66vd2mdu	454	1	MedImmune	MedImmune	NNP
cord-014597-66vd2mdu	454	2	has	have	VBZ
cord-014597-66vd2mdu	454	3	successfully	successfully	RB
cord-014597-66vd2mdu	454	4	completed	complete	VBN
cord-014597-66vd2mdu	454	5	the	the	DT
cord-014597-66vd2mdu	454	6	first	first	RB
cord-014597-66vd2mdu	454	7	known	known	JJ
cord-014597-66vd2mdu	454	8	successful	successful	JJ
cord-014597-66vd2mdu	454	9	CHO	CHO	NNP
cord-014597-66vd2mdu	454	10	transient	transient	JJ
cord-014597-66vd2mdu	454	11	culture	culture	NN
cord-014597-66vd2mdu	454	12	at	at	IN
cord-014597-66vd2mdu	454	13	500L	500l	CD
cord-014597-66vd2mdu	454	14	scale	scale	NN
cord-014597-66vd2mdu	454	15	producing	produce	VBG
cord-014597-66vd2mdu	454	16	>	>	XX
cord-014597-66vd2mdu	454	17	800mg	800mg	NNP
cord-014597-66vd2mdu	454	18	/	/	SYM
cord-014597-66vd2mdu	454	19	L	l	NN
cord-014597-66vd2mdu	454	20	of	of	IN
cord-014597-66vd2mdu	454	21	mAb	mab	NN
cord-014597-66vd2mdu	454	22	at	at	IN
cord-014597-66vd2mdu	454	23	harvest	harvest	NN
cord-014597-66vd2mdu	454	24	.	.	.
cord-014597-66vd2mdu	455	1	Process	process	NN
cord-014597-66vd2mdu	455	2	optimisation	optimisation	NN
cord-014597-66vd2mdu	455	3	has	have	VBZ
cord-014597-66vd2mdu	455	4	subsequently	subsequently	RB
cord-014597-66vd2mdu	455	5	demonstrated	demonstrate	VBN
cord-014597-66vd2mdu	455	6	reproducible	reproducible	JJ
cord-014597-66vd2mdu	455	7	titres	titre	NNS
cord-014597-66vd2mdu	455	8	at	at	IN
cord-014597-66vd2mdu	455	9	50L	50l	CD
cord-014597-66vd2mdu	455	10	to	to	IN
cord-014597-66vd2mdu	455	11	250L	250l	CD
cord-014597-66vd2mdu	455	12	scale	scale	VB
cord-014597-66vd2mdu	455	13	exceeding	exceed	VBG
cord-014597-66vd2mdu	455	14	1g	1g	CD
cord-014597-66vd2mdu	455	15	/	/	SYM
cord-014597-66vd2mdu	455	16	L	l	NN
cord-014597-66vd2mdu	455	17	with	with	IN
cord-014597-66vd2mdu	455	18	comparable	comparable	JJ
cord-014597-66vd2mdu	455	19	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	455	20	profiles	profile	NNS
cord-014597-66vd2mdu	455	21	between	between	IN
cord-014597-66vd2mdu	455	22	SUB	sub	NN
cord-014597-66vd2mdu	455	23	and	and	CC
cord-014597-66vd2mdu	455	24	RBr	rbr	NN
cord-014597-66vd2mdu	455	25	cultures	culture	NNS
cord-014597-66vd2mdu	455	26	across	across	IN
cord-014597-66vd2mdu	455	27	scales	scale	NNS
cord-014597-66vd2mdu	455	28	.	.	.
cord-014597-66vd2mdu	456	1	Comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	456	2	analysis	analysis	NN
cord-014597-66vd2mdu	456	3	of	of	IN
cord-014597-66vd2mdu	456	4	the	the	DT
cord-014597-66vd2mdu	456	5	impact	impact	NN
cord-014597-66vd2mdu	456	6	of	of	IN
cord-014597-66vd2mdu	456	7	trace	trace	NN
cord-014597-66vd2mdu	456	8	elements	element	NNS
cord-014597-66vd2mdu	456	9	in	in	IN
cord-014597-66vd2mdu	456	10	media	medium	NNS
cord-014597-66vd2mdu	456	11	on	on	IN
cord-014597-66vd2mdu	456	12	clone	clone	NN
cord-014597-66vd2mdu	456	13	dependent	dependent	JJ
cord-014597-66vd2mdu	456	14	process	process	NN
cord-014597-66vd2mdu	456	15	performance	performance	NN
cord-014597-66vd2mdu	456	16	and	and	CC
cord-014597-66vd2mdu	456	17	product	product	NN
cord-014597-66vd2mdu	456	18	quality	quality	NN
cord-014597-66vd2mdu	456	19	Background	Background	NNP
cord-014597-66vd2mdu	456	20	State	State	NNP
cord-014597-66vd2mdu	456	21	-	-	HYPH
cord-014597-66vd2mdu	456	22	of	of	IN
cord-014597-66vd2mdu	456	23	-	-	HYPH
cord-014597-66vd2mdu	456	24	the	the	DT
cord-014597-66vd2mdu	456	25	-	-	HYPH
cord-014597-66vd2mdu	456	26	art	art	NN
cord-014597-66vd2mdu	456	27	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	456	28	processes	process	NNS
cord-014597-66vd2mdu	456	29	are	be	VBP
cord-014597-66vd2mdu	456	30	accounting	account	VBG
cord-014597-66vd2mdu	456	31	concomitantly	concomitantly	RB
cord-014597-66vd2mdu	456	32	for	for	IN
cord-014597-66vd2mdu	456	33	process	process	NN
cord-014597-66vd2mdu	456	34	performance	performance	NN
cord-014597-66vd2mdu	456	35	and	and	CC
cord-014597-66vd2mdu	456	36	product	product	NN
cord-014597-66vd2mdu	456	37	quality	quality	NN
cord-014597-66vd2mdu	456	38	.	.	.
cord-014597-66vd2mdu	457	1	Even	even	RB
cord-014597-66vd2mdu	457	2	though	though	IN
cord-014597-66vd2mdu	457	3	high	high	JJ
cord-014597-66vd2mdu	457	4	yielding	yielding	JJ
cord-014597-66vd2mdu	457	5	,	,	,
cord-014597-66vd2mdu	457	6	robust	robust	JJ
cord-014597-66vd2mdu	457	7	processes	process	NNS
cord-014597-66vd2mdu	457	8	are	be	VBP
cord-014597-66vd2mdu	457	9	the	the	DT
cord-014597-66vd2mdu	457	10	cornerstones	cornerstone	NNS
cord-014597-66vd2mdu	457	11	of	of	IN
cord-014597-66vd2mdu	457	12	any	any	DT
cord-014597-66vd2mdu	457	13	process	process	NN
cord-014597-66vd2mdu	457	14	development	development	NN
cord-014597-66vd2mdu	457	15	,	,	,
cord-014597-66vd2mdu	457	16	product	product	NN
cord-014597-66vd2mdu	457	17	quality	quality	NN
cord-014597-66vd2mdu	457	18	parameters	parameter	NNS
cord-014597-66vd2mdu	457	19	such	such	JJ
cord-014597-66vd2mdu	457	20	as	as	IN
cord-014597-66vd2mdu	457	21	structural	structural	JJ
cord-014597-66vd2mdu	457	22	integrity	integrity	NN
cord-014597-66vd2mdu	457	23	,	,	,
cord-014597-66vd2mdu	457	24	charge	charge	NN
cord-014597-66vd2mdu	457	25	variances	variance	NNS
cord-014597-66vd2mdu	457	26	and	and	CC
cord-014597-66vd2mdu	457	27	post	post	JJ
cord-014597-66vd2mdu	457	28	-	-	JJ
cord-014597-66vd2mdu	457	29	translational	translational	JJ
cord-014597-66vd2mdu	457	30	modifications	modification	NNS
cord-014597-66vd2mdu	457	31	are	be	VBP
cord-014597-66vd2mdu	457	32	progressively	progressively	RB
cord-014597-66vd2mdu	457	33	becoming	become	VBG
cord-014597-66vd2mdu	457	34	the	the	DT
cord-014597-66vd2mdu	457	35	focus	focus	NN
cord-014597-66vd2mdu	457	36	of	of	IN
cord-014597-66vd2mdu	457	37	the	the	DT
cord-014597-66vd2mdu	457	38	developmental	developmental	JJ
cord-014597-66vd2mdu	457	39	work	work	NN
cord-014597-66vd2mdu	457	40	.	.	.
cord-014597-66vd2mdu	458	1	In	in	IN
cord-014597-66vd2mdu	458	2	conjunction	conjunction	NN
cord-014597-66vd2mdu	458	3	with	with	IN
cord-014597-66vd2mdu	458	4	host	host	NN
cord-014597-66vd2mdu	458	5	cell	cell	NN
cord-014597-66vd2mdu	458	6	line	line	NN
cord-014597-66vd2mdu	458	7	selection	selection	NN
cord-014597-66vd2mdu	458	8	and	and	CC
cord-014597-66vd2mdu	458	9	process	process	NN
cord-014597-66vd2mdu	458	10	performance	performance	NN
cord-014597-66vd2mdu	458	11	parameters	parameter	NNS
cord-014597-66vd2mdu	458	12	,	,	,
cord-014597-66vd2mdu	458	13	media	medium	NNS
cord-014597-66vd2mdu	458	14	components	component	NNS
cord-014597-66vd2mdu	458	15	are	be	VBP
cord-014597-66vd2mdu	458	16	crucial	crucial	JJ
cord-014597-66vd2mdu	458	17	for	for	IN
cord-014597-66vd2mdu	458	18	the	the	DT
cord-014597-66vd2mdu	458	19	continued	continued	JJ
cord-014597-66vd2mdu	458	20	progress	progress	NN
cord-014597-66vd2mdu	458	21	in	in	IN
cord-014597-66vd2mdu	458	22	rational	rational	JJ
cord-014597-66vd2mdu	458	23	modulation	modulation	NN
cord-014597-66vd2mdu	458	24	of	of	IN
cord-014597-66vd2mdu	458	25	product	product	NN
cord-014597-66vd2mdu	458	26	quality	quality	NN
cord-014597-66vd2mdu	458	27	attributes	attribute	VBZ
cord-014597-66vd2mdu	458	28	affecting	affect	VBG
cord-014597-66vd2mdu	458	29	biological	biological	JJ
cord-014597-66vd2mdu	458	30	activity	activity	NN
cord-014597-66vd2mdu	458	31	,	,	,
cord-014597-66vd2mdu	458	32	immunogenicity	immunogenicity	NN
cord-014597-66vd2mdu	458	33	,	,	,
cord-014597-66vd2mdu	458	34	half	half	JJ
cord-014597-66vd2mdu	458	35	-	-	HYPH
cord-014597-66vd2mdu	458	36	life	life	NN
cord-014597-66vd2mdu	458	37	or	or	CC
cord-014597-66vd2mdu	458	38	stability	stability	NN
cord-014597-66vd2mdu	458	39	.	.	.
cord-014597-66vd2mdu	459	1	Among	among	IN
cord-014597-66vd2mdu	459	2	media	medium	NNS
cord-014597-66vd2mdu	459	3	components	component	NNS
cord-014597-66vd2mdu	459	4	,	,	,
cord-014597-66vd2mdu	459	5	trace	trace	NN
cord-014597-66vd2mdu	459	6	elements	element	NNS
cord-014597-66vd2mdu	459	7	(	(	-LRB-
cord-014597-66vd2mdu	459	8	TE	TE	NNP
cord-014597-66vd2mdu	459	9	)	)	-RRB-
cord-014597-66vd2mdu	459	10	are	be	VBP
cord-014597-66vd2mdu	459	11	of	of	IN
cord-014597-66vd2mdu	459	12	particular	particular	JJ
cord-014597-66vd2mdu	459	13	interest	interest	NN
cord-014597-66vd2mdu	459	14	as	as	IN
cord-014597-66vd2mdu	459	15	they	-PRON-	PRP
cord-014597-66vd2mdu	459	16	play	play	VBP
cord-014597-66vd2mdu	459	17	a	a	DT
cord-014597-66vd2mdu	459	18	pivotal	pivotal	JJ
cord-014597-66vd2mdu	459	19	role	role	NN
cord-014597-66vd2mdu	459	20	in	in	IN
cord-014597-66vd2mdu	459	21	various	various	JJ
cord-014597-66vd2mdu	459	22	cell	cell	NN
cord-014597-66vd2mdu	459	23	metabolism	metabolism	NN
cord-014597-66vd2mdu	459	24	pathways	pathway	NNS
cord-014597-66vd2mdu	459	25	.	.	.
cord-014597-66vd2mdu	460	1	Based	base	VBN
cord-014597-66vd2mdu	460	2	on	on	IN
cord-014597-66vd2mdu	460	3	a	a	DT
cord-014597-66vd2mdu	460	4	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	460	5	DOE	DOE	NNP
cord-014597-66vd2mdu	460	6	approach	approach	NN
cord-014597-66vd2mdu	460	7	,	,	,
cord-014597-66vd2mdu	460	8	extensive	extensive	JJ
cord-014597-66vd2mdu	460	9	process	process	NN
cord-014597-66vd2mdu	460	10	performance	performance	NN
cord-014597-66vd2mdu	460	11	-	-	:
cord-014597-66vd2mdu	460	12	and	and	CC
cord-014597-66vd2mdu	460	13	product	product	NN
cord-014597-66vd2mdu	460	14	quality	quality	NN
cord-014597-66vd2mdu	460	15	evaluation	evaluation	NN
cord-014597-66vd2mdu	460	16	combined	combine	VBN
cord-014597-66vd2mdu	460	17	with	with	IN
cord-014597-66vd2mdu	460	18	metabolic	metabolic	JJ
cord-014597-66vd2mdu	460	19	flux	flux	NN
cord-014597-66vd2mdu	460	20	analysis	analysis	NN
cord-014597-66vd2mdu	460	21	,	,	,
cord-014597-66vd2mdu	460	22	the	the	DT
cord-014597-66vd2mdu	460	23	impact	impact	NN
cord-014597-66vd2mdu	460	24	of	of	IN
cord-014597-66vd2mdu	460	25	several	several	JJ
cord-014597-66vd2mdu	460	26	trace	trace	NN
cord-014597-66vd2mdu	460	27	elements	element	NNS
cord-014597-66vd2mdu	460	28	on	on	IN
cord-014597-66vd2mdu	460	29	the	the	DT
cord-014597-66vd2mdu	460	30	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	460	31	process	process	NN
cord-014597-66vd2mdu	460	32	is	be	VBZ
cord-014597-66vd2mdu	460	33	assessed	assess	VBN
cord-014597-66vd2mdu	460	34	.	.	.
cord-014597-66vd2mdu	461	1	In	in	IN
cord-014597-66vd2mdu	461	2	a	a	DT
cord-014597-66vd2mdu	461	3	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	461	4	I	i	NN
cord-014597-66vd2mdu	461	5	-	-	HYPH
cord-014597-66vd2mdu	461	6	optimal	optimal	JJ
cord-014597-66vd2mdu	461	7	DOE	DOE	NNP
cord-014597-66vd2mdu	461	8	approach	approach	NN
cord-014597-66vd2mdu	461	9	(	(	-LRB-
cord-014597-66vd2mdu	461	10	Fig	Fig	NNP
cord-014597-66vd2mdu	461	11	.	.	.
cord-014597-66vd2mdu	461	12	1	1	CD
cord-014597-66vd2mdu	461	13	)	)	-RRB-
cord-014597-66vd2mdu	461	14	,	,	,
cord-014597-66vd2mdu	461	15	the	the	DT
cord-014597-66vd2mdu	461	16	effect	effect	NN
cord-014597-66vd2mdu	461	17	of	of	IN
cord-014597-66vd2mdu	461	18	six	six	CD
cord-014597-66vd2mdu	461	19	TE	TE	NNP
cord-014597-66vd2mdu	461	20	in	in	IN
cord-014597-66vd2mdu	461	21	various	various	JJ
cord-014597-66vd2mdu	461	22	concentration	concentration	NN
cord-014597-66vd2mdu	461	23	levels	level	NNS
cord-014597-66vd2mdu	461	24	and	and	CC
cord-014597-66vd2mdu	461	25	combinations	combination	NNS
cord-014597-66vd2mdu	461	26	in	in	IN
cord-014597-66vd2mdu	461	27	serum	serum	NN
cord-014597-66vd2mdu	461	28	-	-	HYPH
cord-014597-66vd2mdu	461	29	free	free	JJ
cord-014597-66vd2mdu	461	30	media	medium	NNS
cord-014597-66vd2mdu	461	31	was	be	VBD
cord-014597-66vd2mdu	461	32	studied	study	VBN
cord-014597-66vd2mdu	461	33	for	for	IN
cord-014597-66vd2mdu	461	34	four	four	CD
cord-014597-66vd2mdu	461	35	different	different	JJ
cord-014597-66vd2mdu	461	36	CHO	CHO	NNP
cord-014597-66vd2mdu	461	37	-	-	HYPH
cord-014597-66vd2mdu	461	38	K1	K1	NNP
cord-014597-66vd2mdu	461	39	cells	cell	NNS
cord-014597-66vd2mdu	461	40	lines	line	NNS
cord-014597-66vd2mdu	461	41	in	in	IN
cord-014597-66vd2mdu	461	42	an	an	DT
cord-014597-66vd2mdu	461	43	ambr	ambr	NNP
cord-014597-66vd2mdu	461	44	®	®	NN
cord-014597-66vd2mdu	461	45	15	15	CD
cord-014597-66vd2mdu	461	46	setup	setup	NN
cord-014597-66vd2mdu	461	47	.	.	.
cord-014597-66vd2mdu	462	1	A	a	DT
cord-014597-66vd2mdu	462	2	scrutiny	scrutiny	NN
cord-014597-66vd2mdu	462	3	of	of	IN
cord-014597-66vd2mdu	462	4	the	the	DT
cord-014597-66vd2mdu	462	5	process	process	NN
cord-014597-66vd2mdu	462	6	performance	performance	NN
cord-014597-66vd2mdu	462	7	parameters	parameter	NNS
cord-014597-66vd2mdu	462	8	such	such	JJ
cord-014597-66vd2mdu	462	9	as	as	IN
cord-014597-66vd2mdu	462	10	cellular	cellular	JJ
cord-014597-66vd2mdu	462	11	growth	growth	NN
cord-014597-66vd2mdu	462	12	,	,	,
cord-014597-66vd2mdu	462	13	productivity	productivity	NN
cord-014597-66vd2mdu	462	14	,	,	,
cord-014597-66vd2mdu	462	15	amino	amino	JJ
cord-014597-66vd2mdu	462	16	acids	acid	NNS
cord-014597-66vd2mdu	462	17	and	and	CC
cord-014597-66vd2mdu	462	18	vitamins	vitamin	NNS
cord-014597-66vd2mdu	462	19	consumptions	consumption	NNS
cord-014597-66vd2mdu	462	20	rates	rate	NNS
cord-014597-66vd2mdu	462	21	for	for	IN
cord-014597-66vd2mdu	462	22	each	each	DT
cord-014597-66vd2mdu	462	23	of	of	IN
cord-014597-66vd2mdu	462	24	the	the	DT
cord-014597-66vd2mdu	462	25	conditions	condition	NNS
cord-014597-66vd2mdu	462	26	was	be	VBD
cord-014597-66vd2mdu	462	27	performed	perform	VBN
cord-014597-66vd2mdu	462	28	.	.	.
cord-014597-66vd2mdu	463	1	The	the	DT
cord-014597-66vd2mdu	463	2	process	process	NN
cord-014597-66vd2mdu	463	3	performance	performance	NN
cord-014597-66vd2mdu	463	4	evaluation	evaluation	NN
cord-014597-66vd2mdu	463	5	was	be	VBD
cord-014597-66vd2mdu	463	6	accompanied	accompany	VBN
cord-014597-66vd2mdu	463	7	by	by	IN
cord-014597-66vd2mdu	463	8	extensive	extensive	JJ
cord-014597-66vd2mdu	463	9	product	product	NN
cord-014597-66vd2mdu	463	10	quality	quality	NN
cord-014597-66vd2mdu	463	11	analysis	analysis	NN
cord-014597-66vd2mdu	463	12	including	include	VBG
cord-014597-66vd2mdu	463	13	size	size	NN
cord-014597-66vd2mdu	463	14	and	and	CC
cord-014597-66vd2mdu	463	15	charge	charge	NN
cord-014597-66vd2mdu	463	16	variants	variant	NNS
cord-014597-66vd2mdu	463	17	,	,	,
cord-014597-66vd2mdu	463	18	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	463	19	patterns	pattern	NNS
cord-014597-66vd2mdu	463	20	,	,	,
cord-014597-66vd2mdu	463	21	oxidation	oxidation	NN
cord-014597-66vd2mdu	463	22	and	and	CC
cord-014597-66vd2mdu	463	23	methylation	methylation	NN
cord-014597-66vd2mdu	463	24	.	.	.
cord-014597-66vd2mdu	464	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	464	2	,	,	,
cord-014597-66vd2mdu	464	3	a	a	DT
cord-014597-66vd2mdu	464	4	metabolic	metabolic	JJ
cord-014597-66vd2mdu	464	5	flux	flux	NN
cord-014597-66vd2mdu	464	6	analysis	analysis	NN
cord-014597-66vd2mdu	464	7	was	be	VBD
cord-014597-66vd2mdu	464	8	performed	perform	VBN
cord-014597-66vd2mdu	464	9	based	base	VBN
cord-014597-66vd2mdu	464	10	on	on	IN
cord-014597-66vd2mdu	464	11	the	the	DT
cord-014597-66vd2mdu	464	12	nitrogen	nitrogen	NN
cord-014597-66vd2mdu	464	13	balance	balance	NN
cord-014597-66vd2mdu	464	14	.	.	.
cord-014597-66vd2mdu	465	1	Based	base	VBN
cord-014597-66vd2mdu	465	2	on	on	IN
cord-014597-66vd2mdu	465	3	extensive	extensive	JJ
cord-014597-66vd2mdu	465	4	analytical	analytical	JJ
cord-014597-66vd2mdu	465	5	data	datum	NNS
cord-014597-66vd2mdu	465	6	,	,	,
cord-014597-66vd2mdu	465	7	the	the	DT
cord-014597-66vd2mdu	465	8	obtained	obtain	VBN
cord-014597-66vd2mdu	465	9	response	response	NN
cord-014597-66vd2mdu	465	10	surface	surface	NN
cord-014597-66vd2mdu	465	11	model	model	NN
cord-014597-66vd2mdu	465	12	provides	provide	VBZ
cord-014597-66vd2mdu	465	13	a	a	DT
cord-014597-66vd2mdu	465	14	clear	clear	JJ
cord-014597-66vd2mdu	465	15	insight	insight	NN
cord-014597-66vd2mdu	465	16	into	into	IN
cord-014597-66vd2mdu	465	17	the	the	DT
cord-014597-66vd2mdu	465	18	impact	impact	NN
cord-014597-66vd2mdu	465	19	of	of	IN
cord-014597-66vd2mdu	465	20	particular	particular	JJ
cord-014597-66vd2mdu	465	21	TE	TE	NNP
cord-014597-66vd2mdu	465	22	and	and	CC
cord-014597-66vd2mdu	465	23	their	-PRON-	PRP$
cord-014597-66vd2mdu	465	24	combinations	combination	NNS
cord-014597-66vd2mdu	465	25	on	on	IN
cord-014597-66vd2mdu	465	26	process	process	NN
cord-014597-66vd2mdu	465	27	performance	performance	NN
cord-014597-66vd2mdu	465	28	and	and	CC
cord-014597-66vd2mdu	465	29	product	product	NN
cord-014597-66vd2mdu	465	30	quality	quality	NN
cord-014597-66vd2mdu	465	31	.	.	.
cord-014597-66vd2mdu	466	1	The	the	DT
cord-014597-66vd2mdu	466	2	high	high	JJ
cord-014597-66vd2mdu	466	3	model	model	NN
cord-014597-66vd2mdu	466	4	quality	quality	NN
cord-014597-66vd2mdu	466	5	enables	enable	VBZ
cord-014597-66vd2mdu	466	6	discriminations	discrimination	NNS
cord-014597-66vd2mdu	466	7	between	between	IN
cord-014597-66vd2mdu	466	8	clone	clone	NN
cord-014597-66vd2mdu	466	9	dependent	dependent	JJ
cord-014597-66vd2mdu	466	10	and	and	CC
cord-014597-66vd2mdu	466	11	clone	clone	VB
cord-014597-66vd2mdu	466	12	independent	independent	JJ
cord-014597-66vd2mdu	466	13	effects	effect	NNS
cord-014597-66vd2mdu	466	14	.	.	.
cord-014597-66vd2mdu	467	1	With	with	IN
cord-014597-66vd2mdu	467	2	an	an	DT
cord-014597-66vd2mdu	467	3	elevation	elevation	NN
cord-014597-66vd2mdu	467	4	in	in	IN
cord-014597-66vd2mdu	467	5	titer	titer	NN
cord-014597-66vd2mdu	467	6	up	up	IN
cord-014597-66vd2mdu	467	7	to	to	TO
cord-014597-66vd2mdu	467	8	25	25	CD
cord-014597-66vd2mdu	467	9	%	%	NN
cord-014597-66vd2mdu	467	10	in	in	IN
cord-014597-66vd2mdu	467	11	the	the	DT
cord-014597-66vd2mdu	467	12	best	good	JJS
cord-014597-66vd2mdu	467	13	condition	condition	NN
cord-014597-66vd2mdu	467	14	of	of	IN
cord-014597-66vd2mdu	467	15	the	the	DT
cord-014597-66vd2mdu	467	16	cell	cell	NN
cord-014597-66vd2mdu	467	17	lines	line	NNS
cord-014597-66vd2mdu	467	18	clearly	clearly	RB
cord-014597-66vd2mdu	467	19	show	show	VBP
cord-014597-66vd2mdu	467	20	,	,	,
cord-014597-66vd2mdu	467	21	that	that	IN
cord-014597-66vd2mdu	467	22	even	even	RB
cord-014597-66vd2mdu	467	23	state	state	NN
cord-014597-66vd2mdu	467	24	-	-	HYPH
cord-014597-66vd2mdu	467	25	of	of	IN
cord-014597-66vd2mdu	467	26	-	-	HYPH
cord-014597-66vd2mdu	467	27	the	the	DT
cord-014597-66vd2mdu	467	28	-	-	HYPH
cord-014597-66vd2mdu	467	29	art	art	NN
cord-014597-66vd2mdu	467	30	media	medium	NNS
cord-014597-66vd2mdu	467	31	can	can	MD
cord-014597-66vd2mdu	467	32	be	be	VB
cord-014597-66vd2mdu	467	33	outperformed	outperform	VBN
cord-014597-66vd2mdu	467	34	by	by	IN
cord-014597-66vd2mdu	467	35	trace	trace	NN
cord-014597-66vd2mdu	467	36	element	element	NN
cord-014597-66vd2mdu	467	37	rebalancing	rebalancing	NN
cord-014597-66vd2mdu	467	38	.	.	.
cord-014597-66vd2mdu	468	1	Analyzing	analyze	VBG
cord-014597-66vd2mdu	468	2	specific	specific	JJ
cord-014597-66vd2mdu	468	3	rates	rate	NNS
cord-014597-66vd2mdu	468	4	in	in	IN
cord-014597-66vd2mdu	468	5	combination	combination	NN
cord-014597-66vd2mdu	468	6	with	with	IN
cord-014597-66vd2mdu	468	7	metabolic	metabolic	JJ
cord-014597-66vd2mdu	468	8	flux	flux	NN
cord-014597-66vd2mdu	468	9	analysis	analysis	NN
cord-014597-66vd2mdu	468	10	improves	improve	VBZ
cord-014597-66vd2mdu	468	11	the	the	DT
cord-014597-66vd2mdu	468	12	understanding	understanding	NN
cord-014597-66vd2mdu	468	13	of	of	IN
cord-014597-66vd2mdu	468	14	metabolic	metabolic	JJ
cord-014597-66vd2mdu	468	15	restructuring	restructuring	NN
cord-014597-66vd2mdu	468	16	of	of	IN
cord-014597-66vd2mdu	468	17	the	the	DT
cord-014597-66vd2mdu	468	18	cell	cell	NN
cord-014597-66vd2mdu	468	19	lines	line	NNS
cord-014597-66vd2mdu	468	20	under	under	IN
cord-014597-66vd2mdu	468	21	distinct	distinct	JJ
cord-014597-66vd2mdu	468	22	TE	TE	NNP
cord-014597-66vd2mdu	468	23	levels	level	NNS
cord-014597-66vd2mdu	468	24	and	and	CC
cord-014597-66vd2mdu	468	25	combinations	combination	NNS
cord-014597-66vd2mdu	468	26	.	.	.
cord-014597-66vd2mdu	469	1	Modulation	modulation	NN
cord-014597-66vd2mdu	469	2	of	of	IN
cord-014597-66vd2mdu	469	3	trace	trace	NN
cord-014597-66vd2mdu	469	4	elements	element	NNS
cord-014597-66vd2mdu	469	5	levels	level	NNS
cord-014597-66vd2mdu	469	6	had	have	VBD
cord-014597-66vd2mdu	469	7	a	a	DT
cord-014597-66vd2mdu	469	8	tremendous	tremendous	JJ
cord-014597-66vd2mdu	469	9	effect	effect	NN
cord-014597-66vd2mdu	469	10	on	on	IN
cord-014597-66vd2mdu	469	11	the	the	DT
cord-014597-66vd2mdu	469	12	charge	charge	NN
cord-014597-66vd2mdu	469	13	heterogeneity	heterogeneity	NN
cord-014597-66vd2mdu	469	14	and	and	CC
cord-014597-66vd2mdu	469	15	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	469	16	structure	structure	NN
cord-014597-66vd2mdu	469	17	of	of	IN
cord-014597-66vd2mdu	469	18	the	the	DT
cord-014597-66vd2mdu	469	19	different	different	JJ
cord-014597-66vd2mdu	469	20	proteins	protein	NNS
cord-014597-66vd2mdu	469	21	.	.	.
cord-014597-66vd2mdu	470	1	This	this	DT
cord-014597-66vd2mdu	470	2	provides	provide	VBZ
cord-014597-66vd2mdu	470	3	a	a	DT
cord-014597-66vd2mdu	470	4	toolbox	toolbox	NN
cord-014597-66vd2mdu	470	5	for	for	IN
cord-014597-66vd2mdu	470	6	the	the	DT
cord-014597-66vd2mdu	470	7	fine	fine	JJ
cord-014597-66vd2mdu	470	8	tuning	tuning	NN
cord-014597-66vd2mdu	470	9	and	and	CC
cord-014597-66vd2mdu	470	10	control	control	NN
cord-014597-66vd2mdu	470	11	of	of	IN
cord-014597-66vd2mdu	470	12	product	product	NN
cord-014597-66vd2mdu	470	13	quality	quality	NN
cord-014597-66vd2mdu	470	14	parameters	parameter	NNS
cord-014597-66vd2mdu	470	15	.	.	.
cord-014597-66vd2mdu	471	1	Taken	take	VBN
cord-014597-66vd2mdu	471	2	together	together	RB
cord-014597-66vd2mdu	471	3	,	,	,
cord-014597-66vd2mdu	471	4	the	the	DT
cord-014597-66vd2mdu	471	5	data	datum	NNS
cord-014597-66vd2mdu	471	6	further	further	RB
cord-014597-66vd2mdu	471	7	paves	pave	VBZ
cord-014597-66vd2mdu	471	8	the	the	DT
cord-014597-66vd2mdu	471	9	way	way	NN
cord-014597-66vd2mdu	471	10	to	to	IN
cord-014597-66vd2mdu	471	11	the	the	DT
cord-014597-66vd2mdu	471	12	rational	rational	JJ
cord-014597-66vd2mdu	471	13	fine	fine	JJ
cord-014597-66vd2mdu	471	14	tuning	tuning	NN
cord-014597-66vd2mdu	471	15	of	of	IN
cord-014597-66vd2mdu	471	16	process	process	NN
cord-014597-66vd2mdu	471	17	performance	performance	NN
cord-014597-66vd2mdu	471	18	and	and	CC
cord-014597-66vd2mdu	471	19	product	product	NN
cord-014597-66vd2mdu	471	20	quality	quality	NN
cord-014597-66vd2mdu	471	21	attributes	attribute	NNS
cord-014597-66vd2mdu	471	22	.	.	.
cord-014597-66vd2mdu	472	1	Background	background	NN
cord-014597-66vd2mdu	472	2	Due	due	IN
cord-014597-66vd2mdu	472	3	to	to	IN
cord-014597-66vd2mdu	472	4	regulatory	regulatory	JJ
cord-014597-66vd2mdu	472	5	concerns	concern	NNS
cord-014597-66vd2mdu	472	6	and	and	CC
cord-014597-66vd2mdu	472	7	economic	economic	JJ
cord-014597-66vd2mdu	472	8	impact	impact	NN
cord-014597-66vd2mdu	472	9	,	,	,
cord-014597-66vd2mdu	472	10	ensuring	ensure	VBG
cord-014597-66vd2mdu	472	11	product	product	NN
cord-014597-66vd2mdu	472	12	quality	quality	NN
cord-014597-66vd2mdu	472	13	and	and	CC
cord-014597-66vd2mdu	472	14	consistency	consistency	NN
cord-014597-66vd2mdu	472	15	is	be	VBZ
cord-014597-66vd2mdu	472	16	now	now	RB
cord-014597-66vd2mdu	472	17	one	one	CD
cord-014597-66vd2mdu	472	18	of	of	IN
cord-014597-66vd2mdu	472	19	the	the	DT
cord-014597-66vd2mdu	472	20	main	main	JJ
cord-014597-66vd2mdu	472	21	challenge	challenge	NN
cord-014597-66vd2mdu	472	22	faced	face	VBN
cord-014597-66vd2mdu	472	23	by	by	IN
cord-014597-66vd2mdu	472	24	the	the	DT
cord-014597-66vd2mdu	472	25	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	472	26	industry	industry	NN
cord-014597-66vd2mdu	472	27	.	.	.
cord-014597-66vd2mdu	473	1	For	for	IN
cord-014597-66vd2mdu	473	2	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	473	3	antibodies	antibody	NNS
cord-014597-66vd2mdu	473	4	(	(	-LRB-
cord-014597-66vd2mdu	473	5	mAb	mAb	NNS
cord-014597-66vd2mdu	473	6	)	)	-RRB-
cord-014597-66vd2mdu	473	7	,	,	,
cord-014597-66vd2mdu	473	8	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	473	9	is	be	VBZ
cord-014597-66vd2mdu	473	10	one	one	CD
cord-014597-66vd2mdu	473	11	of	of	IN
cord-014597-66vd2mdu	473	12	the	the	DT
cord-014597-66vd2mdu	473	13	most	most	RBS
cord-014597-66vd2mdu	473	14	important	important	JJ
cord-014597-66vd2mdu	473	15	quality	quality	NN
cord-014597-66vd2mdu	473	16	attributes	attribute	NNS
cord-014597-66vd2mdu	473	17	as	as	IN
cord-014597-66vd2mdu	473	18	it	-PRON-	PRP
cord-014597-66vd2mdu	473	19	impacts	impact	VBZ
cord-014597-66vd2mdu	473	20	on	on	IN
cord-014597-66vd2mdu	473	21	mAb	mab	NN
cord-014597-66vd2mdu	473	22	structure	structure	NN
cord-014597-66vd2mdu	473	23	integrity	integrity	NN
cord-014597-66vd2mdu	473	24	,	,	,
cord-014597-66vd2mdu	473	25	and	and	CC
cord-014597-66vd2mdu	473	26	ultimately	ultimately	RB
cord-014597-66vd2mdu	473	27	on	on	IN
cord-014597-66vd2mdu	473	28	both	both	CC
cord-014597-66vd2mdu	473	29	clinical	clinical	JJ
cord-014597-66vd2mdu	473	30	efficacy	efficacy	NN
cord-014597-66vd2mdu	473	31	and	and	CC
cord-014597-66vd2mdu	473	32	safety	safety	NN
cord-014597-66vd2mdu	473	33	.	.	.
cord-014597-66vd2mdu	474	1	Many	many	JJ
cord-014597-66vd2mdu	474	2	factors	factor	NNS
cord-014597-66vd2mdu	474	3	affect	affect	VBP
cord-014597-66vd2mdu	474	4	mAb	mab	NN
cord-014597-66vd2mdu	474	5	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	474	6	and	and	CC
cord-014597-66vd2mdu	474	7	its	-PRON-	PRP$
cord-014597-66vd2mdu	474	8	inherent	inherent	JJ
cord-014597-66vd2mdu	474	9	heterogeneity	heterogeneity	NN
cord-014597-66vd2mdu	474	10	,	,	,
cord-014597-66vd2mdu	474	11	including	include	VBG
cord-014597-66vd2mdu	474	12	the	the	DT
cord-014597-66vd2mdu	474	13	host	host	NN
cord-014597-66vd2mdu	474	14	cell	cell	NN
cord-014597-66vd2mdu	474	15	,	,	,
cord-014597-66vd2mdu	474	16	the	the	DT
cord-014597-66vd2mdu	474	17	culture	culture	NN
cord-014597-66vd2mdu	474	18	medium	medium	NN
cord-014597-66vd2mdu	474	19	,	,	,
cord-014597-66vd2mdu	474	20	the	the	DT
cord-014597-66vd2mdu	474	21	mode	mode	NN
cord-014597-66vd2mdu	474	22	of	of	IN
cord-014597-66vd2mdu	474	23	operation	operation	NN
cord-014597-66vd2mdu	474	24	and	and	CC
cord-014597-66vd2mdu	474	25	the	the	DT
cord-014597-66vd2mdu	474	26	operating	operating	NN
cord-014597-66vd2mdu	474	27	conditions	condition	NNS
cord-014597-66vd2mdu	474	28	.	.	.
cord-014597-66vd2mdu	475	1	In	in	IN
cord-014597-66vd2mdu	475	2	this	this	DT
cord-014597-66vd2mdu	475	3	context	context	NN
cord-014597-66vd2mdu	475	4	,	,	,
cord-014597-66vd2mdu	475	5	the	the	DT
cord-014597-66vd2mdu	475	6	capacity	capacity	NN
cord-014597-66vd2mdu	475	7	to	to	TO
cord-014597-66vd2mdu	475	8	monitor	monitor	VB
cord-014597-66vd2mdu	475	9	and	and	CC
cord-014597-66vd2mdu	475	10	control	control	VB
cord-014597-66vd2mdu	475	11	on	on	IN
cord-014597-66vd2mdu	475	12	-	-	HYPH
cord-014597-66vd2mdu	475	13	line	line	NN
cord-014597-66vd2mdu	475	14	the	the	DT
cord-014597-66vd2mdu	475	15	antibody	antibody	NN
cord-014597-66vd2mdu	475	16	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	475	17	,	,	,
cord-014597-66vd2mdu	475	18	from	from	IN
cord-014597-66vd2mdu	475	19	early	early	JJ
cord-014597-66vd2mdu	475	20	-	-	HYPH
cord-014597-66vd2mdu	475	21	to	to	IN
cord-014597-66vd2mdu	475	22	late	late	JJ
cord-014597-66vd2mdu	475	23	-	-	HYPH
cord-014597-66vd2mdu	475	24	stage	stage	NN
cord-014597-66vd2mdu	475	25	process	process	NN
cord-014597-66vd2mdu	475	26	development	development	NN
cord-014597-66vd2mdu	475	27	,	,	,
cord-014597-66vd2mdu	475	28	would	would	MD
cord-014597-66vd2mdu	475	29	be	be	VB
cord-014597-66vd2mdu	475	30	of	of	IN
cord-014597-66vd2mdu	475	31	salient	salient	JJ
cord-014597-66vd2mdu	475	32	interest	interest	NN
cord-014597-66vd2mdu	475	33	to	to	TO
cord-014597-66vd2mdu	475	34	reduce	reduce	VB
cord-014597-66vd2mdu	475	35	the	the	DT
cord-014597-66vd2mdu	475	36	time	time	NN
cord-014597-66vd2mdu	475	37	and	and	CC
cord-014597-66vd2mdu	475	38	cost	cost	VBD
cord-014597-66vd2mdu	475	39	to	to	IN
cord-014597-66vd2mdu	475	40	market	market	NN
cord-014597-66vd2mdu	475	41	.	.	.
cord-014597-66vd2mdu	476	1	In	in	IN
cord-014597-66vd2mdu	476	2	order	order	NN
cord-014597-66vd2mdu	476	3	to	to	TO
cord-014597-66vd2mdu	476	4	address	address	VB
cord-014597-66vd2mdu	476	5	this	this	DT
cord-014597-66vd2mdu	476	6	unmet	unmet	JJ
cord-014597-66vd2mdu	476	7	need	need	NN
cord-014597-66vd2mdu	476	8	,	,	,
cord-014597-66vd2mdu	476	9	we	-PRON-	PRP
cord-014597-66vd2mdu	476	10	have	have	VBP
cord-014597-66vd2mdu	476	11	designed	design	VBN
cord-014597-66vd2mdu	476	12	an	an	DT
cord-014597-66vd2mdu	476	13	improved	improve	VBN
cord-014597-66vd2mdu	476	14	SPR	SPR	NNP
cord-014597-66vd2mdu	476	15	biosensor	biosensor	NN
cord-014597-66vd2mdu	476	16	assay	assay	NN
cord-014597-66vd2mdu	476	17	to	to	TO
cord-014597-66vd2mdu	476	18	measure	measure	VB
cord-014597-66vd2mdu	476	19	the	the	DT
cord-014597-66vd2mdu	476	20	kinetics	kinetic	NNS
cord-014597-66vd2mdu	476	21	of	of	IN
cord-014597-66vd2mdu	476	22	interaction	interaction	NN
cord-014597-66vd2mdu	476	23	between	between	IN
cord-014597-66vd2mdu	476	24	a	a	DT
cord-014597-66vd2mdu	476	25	mAb	mAb	NNS
cord-014597-66vd2mdu	476	26	and	and	CC
cord-014597-66vd2mdu	476	27	the	the	DT
cord-014597-66vd2mdu	476	28	extracellular	extracellular	JJ
cord-014597-66vd2mdu	476	29	domain	domain	NN
cord-014597-66vd2mdu	476	30	of	of	IN
cord-014597-66vd2mdu	476	31	the	the	DT
cord-014597-66vd2mdu	476	32	FcγRIIIa	FcγRIIIa	NNP
cord-014597-66vd2mdu	476	33	receptor	receptor	NN
cord-014597-66vd2mdu	476	34	bound	bind	VBN
cord-014597-66vd2mdu	476	35	at	at	IN
cord-014597-66vd2mdu	476	36	the	the	DT
cord-014597-66vd2mdu	476	37	biosensor	biosensor	NN
cord-014597-66vd2mdu	476	38	surface	surface	NN
cord-014597-66vd2mdu	476	39	[	[	-LRB-
cord-014597-66vd2mdu	476	40	1	1	CD
cord-014597-66vd2mdu	476	41	,	,	,
cord-014597-66vd2mdu	476	42	2	2	CD
cord-014597-66vd2mdu	476	43	]	]	-RRB-
cord-014597-66vd2mdu	476	44	.	.	.
cord-014597-66vd2mdu	477	1	Of	of	IN
cord-014597-66vd2mdu	477	2	salient	salient	JJ
cord-014597-66vd2mdu	477	3	interest	interest	NN
cord-014597-66vd2mdu	477	4	,	,	,
cord-014597-66vd2mdu	477	5	we	-PRON-	PRP
cord-014597-66vd2mdu	477	6	also	also	RB
cord-014597-66vd2mdu	477	7	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	477	8	that	that	IN
cord-014597-66vd2mdu	477	9	various	various	JJ
cord-014597-66vd2mdu	477	10	binding	bind	VBG
cord-014597-66vd2mdu	477	11	kinetic	kinetic	JJ
cord-014597-66vd2mdu	477	12	signatures	signature	NNS
cord-014597-66vd2mdu	477	13	,	,	,
cord-014597-66vd2mdu	477	14	especially	especially	RB
cord-014597-66vd2mdu	477	15	different	different	JJ
cord-014597-66vd2mdu	477	16	dissociation	dissociation	NN
cord-014597-66vd2mdu	477	17	kinetics	kinetics	NN
cord-014597-66vd2mdu	477	18	could	could	MD
cord-014597-66vd2mdu	477	19	be	be	VB
cord-014597-66vd2mdu	477	20	correlated	correlate	VBN
cord-014597-66vd2mdu	477	21	with	with	IN
cord-014597-66vd2mdu	477	22	distinct	distinct	JJ
cord-014597-66vd2mdu	477	23	mAb	mAb	HYPH
cord-014597-66vd2mdu	477	24	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	477	25	patterns	pattern	NNS
cord-014597-66vd2mdu	477	26	and	and	CC
cord-014597-66vd2mdu	477	27	with	with	IN
cord-014597-66vd2mdu	477	28	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	477	29	efficacies	efficacy	NNS
cord-014597-66vd2mdu	477	30	,	,	,
cord-014597-66vd2mdu	477	31	as	as	IN
cord-014597-66vd2mdu	477	32	deduced	deduce	VBN
cord-014597-66vd2mdu	477	33	from	from	IN
cord-014597-66vd2mdu	477	34	mass	mass	NN
cord-014597-66vd2mdu	477	35	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	477	36	and	and	CC
cord-014597-66vd2mdu	477	37	a	a	DT
cord-014597-66vd2mdu	477	38	surrogate	surrogate	JJ
cord-014597-66vd2mdu	477	39	ADCC	ADCC	NNP
cord-014597-66vd2mdu	477	40	assay	assay	NN
cord-014597-66vd2mdu	477	41	,	,	,
cord-014597-66vd2mdu	477	42	respectively	respectively	RB
cord-014597-66vd2mdu	477	43	.	.	.
cord-014597-66vd2mdu	478	1	In	in	IN
cord-014597-66vd2mdu	478	2	parallel	parallel	NN
cord-014597-66vd2mdu	478	3	,	,	,
cord-014597-66vd2mdu	478	4	we	-PRON-	PRP
cord-014597-66vd2mdu	478	5	have	have	VBP
cord-014597-66vd2mdu	478	6	also	also	RB
cord-014597-66vd2mdu	478	7	harnessed	harness	VBN
cord-014597-66vd2mdu	478	8	a	a	DT
cord-014597-66vd2mdu	478	9	SPR	SPR	NNP
cord-014597-66vd2mdu	478	10	biosensor	biosensor	NN
cord-014597-66vd2mdu	478	11	directly	directly	RB
cord-014597-66vd2mdu	478	12	to	to	IN
cord-014597-66vd2mdu	478	13	a	a	DT
cord-014597-66vd2mdu	478	14	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	478	15	,	,	,
cord-014597-66vd2mdu	478	16	which	which	WDT
cord-014597-66vd2mdu	478	17	permitted	permit	VBD
cord-014597-66vd2mdu	478	18	the	the	DT
cord-014597-66vd2mdu	478	19	at	at	IN
cord-014597-66vd2mdu	478	20	-	-	HYPH
cord-014597-66vd2mdu	478	21	line	line	NN
cord-014597-66vd2mdu	478	22	determination	determination	NN
cord-014597-66vd2mdu	478	23	of	of	IN
cord-014597-66vd2mdu	478	24	the	the	DT
cord-014597-66vd2mdu	478	25	concentration	concentration	NN
cord-014597-66vd2mdu	478	26	of	of	IN
cord-014597-66vd2mdu	478	27	antibodies	antibody	NNS
cord-014597-66vd2mdu	478	28	by	by	IN
cord-014597-66vd2mdu	478	29	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	478	30	cells	cell	NNS
cord-014597-66vd2mdu	478	31	during	during	IN
cord-014597-66vd2mdu	478	32	a	a	DT
cord-014597-66vd2mdu	478	33	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	478	34	culture	culture	NN
cord-014597-66vd2mdu	478	35	.	.	.
cord-014597-66vd2mdu	479	1	We	-PRON-	PRP
cord-014597-66vd2mdu	479	2	now	now	RB
cord-014597-66vd2mdu	479	3	plan	plan	VBP
cord-014597-66vd2mdu	479	4	on	on	IN
cord-014597-66vd2mdu	479	5	combining	combine	VBG
cord-014597-66vd2mdu	479	6	both	both	DT
cord-014597-66vd2mdu	479	7	approaches	approach	NNS
cord-014597-66vd2mdu	479	8	to	to	TO
cord-014597-66vd2mdu	479	9	determine	determine	VB
cord-014597-66vd2mdu	479	10	on	on	IN
cord-014597-66vd2mdu	479	11	-	-	HYPH
cord-014597-66vd2mdu	479	12	line	line	NN
cord-014597-66vd2mdu	479	13	the	the	DT
cord-014597-66vd2mdu	479	14	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	479	15	profile	profile	NN
cord-014597-66vd2mdu	479	16	of	of	IN
cord-014597-66vd2mdu	479	17	the	the	DT
cord-014597-66vd2mdu	479	18	produced	produce	VBN
cord-014597-66vd2mdu	479	19	mAbs	mabs	NN
cord-014597-66vd2mdu	479	20	.	.	.
cord-014597-66vd2mdu	480	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	480	2	ultimate	ultimate	JJ
cord-014597-66vd2mdu	480	3	goal	goal	NN
cord-014597-66vd2mdu	480	4	is	be	VBZ
cord-014597-66vd2mdu	480	5	to	to	TO
cord-014597-66vd2mdu	480	6	design	design	VB
cord-014597-66vd2mdu	480	7	a	a	DT
cord-014597-66vd2mdu	480	8	unique	unique	JJ
cord-014597-66vd2mdu	480	9	and	and	CC
cord-014597-66vd2mdu	480	10	highly	highly	RB
cord-014597-66vd2mdu	480	11	innovative	innovative	JJ
cord-014597-66vd2mdu	480	12	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	480	13	control	control	NN
cord-014597-66vd2mdu	480	14	tool	tool	NN
cord-014597-66vd2mdu	480	15	that	that	WDT
cord-014597-66vd2mdu	480	16	can	can	MD
cord-014597-66vd2mdu	480	17	be	be	VB
cord-014597-66vd2mdu	480	18	readily	readily	RB
cord-014597-66vd2mdu	480	19	applied	apply	VBN
cord-014597-66vd2mdu	480	20	in	in	IN
cord-014597-66vd2mdu	480	21	an	an	DT
cord-014597-66vd2mdu	480	22	industrial	industrial	JJ
cord-014597-66vd2mdu	480	23	bio	bio	NN
cord-014597-66vd2mdu	480	24	-	-	HYPH
cord-014597-66vd2mdu	480	25	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	480	26	setting	setting	NN
cord-014597-66vd2mdu	480	27	.	.	.
cord-014597-66vd2mdu	481	1	Reducing	reduce	VBG
cord-014597-66vd2mdu	481	2	timelines	timeline	NNS
cord-014597-66vd2mdu	481	3	and	and	CC
cord-014597-66vd2mdu	481	4	costs	cost	NNS
cord-014597-66vd2mdu	481	5	are	be	VBP
cord-014597-66vd2mdu	481	6	key	key	JJ
cord-014597-66vd2mdu	481	7	factors	factor	NNS
cord-014597-66vd2mdu	481	8	for	for	IN
cord-014597-66vd2mdu	481	9	bio	bio	JJ
cord-014597-66vd2mdu	481	10	-	-	JJ
cord-014597-66vd2mdu	481	11	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	481	12	industries	industry	NNS
cord-014597-66vd2mdu	481	13	to	to	TO
cord-014597-66vd2mdu	481	14	accelerate	accelerate	VB
cord-014597-66vd2mdu	481	15	process	process	NN
cord-014597-66vd2mdu	481	16	development	development	NN
cord-014597-66vd2mdu	481	17	and	and	CC
cord-014597-66vd2mdu	481	18	drug	drug	NN
cord-014597-66vd2mdu	481	19	delivery	delivery	NN
cord-014597-66vd2mdu	481	20	to	to	IN
cord-014597-66vd2mdu	481	21	patients	patient	NNS
cord-014597-66vd2mdu	481	22	.	.	.
cord-014597-66vd2mdu	482	1	Enhancing	enhance	VBG
cord-014597-66vd2mdu	482	2	throughput	throughput	NN
cord-014597-66vd2mdu	482	3	of	of	IN
cord-014597-66vd2mdu	482	4	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	482	5	development	development	NN
cord-014597-66vd2mdu	482	6	has	have	VBZ
cord-014597-66vd2mdu	482	7	become	become	VBN
cord-014597-66vd2mdu	482	8	increasingly	increasingly	RB
cord-014597-66vd2mdu	482	9	important	important	JJ
cord-014597-66vd2mdu	482	10	for	for	IN
cord-014597-66vd2mdu	482	11	the	the	DT
cord-014597-66vd2mdu	482	12	screening	screening	NN
cord-014597-66vd2mdu	482	13	and	and	CC
cord-014597-66vd2mdu	482	14	optimization	optimization	NN
cord-014597-66vd2mdu	482	15	of	of	IN
cord-014597-66vd2mdu	482	16	cell	cell	NN
cord-014597-66vd2mdu	482	17	culture	culture	NN
cord-014597-66vd2mdu	482	18	processes	process	NNS
cord-014597-66vd2mdu	482	19	.	.	.
cord-014597-66vd2mdu	483	1	This	this	DT
cord-014597-66vd2mdu	483	2	challenge	challenge	NN
cord-014597-66vd2mdu	483	3	requires	require	VBZ
cord-014597-66vd2mdu	483	4	high	high	JJ
cord-014597-66vd2mdu	483	5	throughput	throughput	NN
cord-014597-66vd2mdu	483	6	tools	tool	NNS
cord-014597-66vd2mdu	483	7	.	.	.
cord-014597-66vd2mdu	484	1	In	in	IN
cord-014597-66vd2mdu	484	2	a	a	DT
cord-014597-66vd2mdu	484	3	previous	previous	JJ
cord-014597-66vd2mdu	484	4	study	study	NN
cord-014597-66vd2mdu	484	5	[	[	-LRB-
cord-014597-66vd2mdu	484	6	1	1	CD
cord-014597-66vd2mdu	484	7	]	]	-RRB-
cord-014597-66vd2mdu	484	8	,	,	,
cord-014597-66vd2mdu	484	9	we	-PRON-	PRP
cord-014597-66vd2mdu	484	10	showed	show	VBD
cord-014597-66vd2mdu	484	11	that	that	IN
cord-014597-66vd2mdu	484	12	ambr	ambr	NNP
cord-014597-66vd2mdu	484	13	®	®	NNP
cord-014597-66vd2mdu	484	14	15	15	CD
cord-014597-66vd2mdu	484	15	,	,	,
cord-014597-66vd2mdu	484	16	a	a	DT
cord-014597-66vd2mdu	484	17	robotically	robotically	RB
cord-014597-66vd2mdu	484	18	driven	drive	VBN
cord-014597-66vd2mdu	484	19	mini	mini	NN
cord-014597-66vd2mdu	484	20	-	-	NN
cord-014597-66vd2mdu	484	21	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	484	22	system	system	NN
cord-014597-66vd2mdu	484	23	developed	develop	VBN
cord-014597-66vd2mdu	484	24	by	by	IN
cord-014597-66vd2mdu	484	25	TAP	TAP	NNP
cord-014597-66vd2mdu	484	26	-	-	HYPH
cord-014597-66vd2mdu	484	27	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	484	28	,	,	,
cord-014597-66vd2mdu	484	29	could	could	MD
cord-014597-66vd2mdu	484	30	be	be	VB
cord-014597-66vd2mdu	484	31	advantageous	advantageous	JJ
cord-014597-66vd2mdu	484	32	to	to	TO
cord-014597-66vd2mdu	484	33	accelerate	accelerate	VB
cord-014597-66vd2mdu	484	34	process	process	NN
cord-014597-66vd2mdu	484	35	development	development	NN
cord-014597-66vd2mdu	484	36	.	.	.
cord-014597-66vd2mdu	485	1	The	the	DT
cord-014597-66vd2mdu	485	2	use	use	NN
cord-014597-66vd2mdu	485	3	of	of	IN
cord-014597-66vd2mdu	485	4	ambr	ambr	NNP
cord-014597-66vd2mdu	485	5	®	®	NNP
cord-014597-66vd2mdu	485	6	15	15	CD
cord-014597-66vd2mdu	485	7	system	system	NN
cord-014597-66vd2mdu	485	8	allows	allow	VBZ
cord-014597-66vd2mdu	485	9	us	-PRON-	PRP
cord-014597-66vd2mdu	485	10	to	to	TO
cord-014597-66vd2mdu	485	11	test	test	VB
cord-014597-66vd2mdu	485	12	a	a	DT
cord-014597-66vd2mdu	485	13	large	large	JJ
cord-014597-66vd2mdu	485	14	number	number	NN
cord-014597-66vd2mdu	485	15	of	of	IN
cord-014597-66vd2mdu	485	16	experimental	experimental	JJ
cord-014597-66vd2mdu	485	17	conditions	condition	NNS
cord-014597-66vd2mdu	485	18	in	in	IN
cord-014597-66vd2mdu	485	19	a	a	DT
cord-014597-66vd2mdu	485	20	single	single	JJ
cord-014597-66vd2mdu	485	21	experiment	experiment	NN
cord-014597-66vd2mdu	485	22	.	.	.
cord-014597-66vd2mdu	486	1	Therefore	therefore	RB
cord-014597-66vd2mdu	486	2	,	,	,
cord-014597-66vd2mdu	486	3	the	the	DT
cord-014597-66vd2mdu	486	4	large	large	JJ
cord-014597-66vd2mdu	486	5	amount	amount	NN
cord-014597-66vd2mdu	486	6	of	of	IN
cord-014597-66vd2mdu	486	7	production	production	NN
cord-014597-66vd2mdu	486	8	samples	sample	NNS
cord-014597-66vd2mdu	486	9	to	to	TO
cord-014597-66vd2mdu	486	10	be	be	VB
cord-014597-66vd2mdu	486	11	characterized	characterize	VBN
cord-014597-66vd2mdu	486	12	for	for	IN
cord-014597-66vd2mdu	486	13	Product	product	NN
cord-014597-66vd2mdu	486	14	Quality	quality	NN
cord-014597-66vd2mdu	486	15	Attributes	Attributes	NNPS
cord-014597-66vd2mdu	486	16	(	(	-LRB-
cord-014597-66vd2mdu	486	17	PQA	PQA	NNP
cord-014597-66vd2mdu	486	18	)	)	-RRB-
cord-014597-66vd2mdu	486	19	increases	increase	VBZ
cord-014597-66vd2mdu	486	20	as	as	RB
cord-014597-66vd2mdu	486	21	well	well	RB
cord-014597-66vd2mdu	486	22	:	:	:
cord-014597-66vd2mdu	486	23	the	the	DT
cord-014597-66vd2mdu	486	24	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	486	25	has	have	VBZ
cord-014597-66vd2mdu	486	26	moved	move	VBN
cord-014597-66vd2mdu	486	27	from	from	IN
cord-014597-66vd2mdu	486	28	the	the	DT
cord-014597-66vd2mdu	486	29	generation	generation	NN
cord-014597-66vd2mdu	486	30	of	of	IN
cord-014597-66vd2mdu	486	31	samples	sample	NNS
cord-014597-66vd2mdu	486	32	at	at	IN
cord-014597-66vd2mdu	486	33	the	the	DT
cord-014597-66vd2mdu	486	34	production	production	NN
cord-014597-66vd2mdu	486	35	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	486	36	step	step	NN
cord-014597-66vd2mdu	486	37	to	to	IN
cord-014597-66vd2mdu	486	38	in	in	IN
cord-014597-66vd2mdu	486	39	-	-	HYPH
cord-014597-66vd2mdu	486	40	process	process	NN
cord-014597-66vd2mdu	486	41	analysis	analysis	NN
cord-014597-66vd2mdu	486	42	.	.	.
cord-014597-66vd2mdu	487	1	For	for	IN
cord-014597-66vd2mdu	487	2	product	product	NN
cord-014597-66vd2mdu	487	3	quality	quality	NN
cord-014597-66vd2mdu	487	4	attribute	attribute	NN
cord-014597-66vd2mdu	487	5	analysis	analysis	NN
cord-014597-66vd2mdu	487	6	at	at	IN
cord-014597-66vd2mdu	487	7	lab	lab	NN
cord-014597-66vd2mdu	487	8	scale	scale	NN
cord-014597-66vd2mdu	487	9	,	,	,
cord-014597-66vd2mdu	487	10	protein	protein	NN
cord-014597-66vd2mdu	487	11	purification	purification	NN
cord-014597-66vd2mdu	487	12	is	be	VBZ
cord-014597-66vd2mdu	487	13	generally	generally	RB
cord-014597-66vd2mdu	487	14	carried	carry	VBN
cord-014597-66vd2mdu	487	15	out	out	RP
cord-014597-66vd2mdu	487	16	on	on	IN
cord-014597-66vd2mdu	487	17	>	>	$
cord-014597-66vd2mdu	487	18	5mL	5ml	CD
cord-014597-66vd2mdu	487	19	columns	column	NNS
cord-014597-66vd2mdu	487	20	which	which	WDT
cord-014597-66vd2mdu	487	21	is	be	VBZ
cord-014597-66vd2mdu	487	22	incompatible	incompatible	JJ
cord-014597-66vd2mdu	487	23	with	with	IN
cord-014597-66vd2mdu	487	24	the	the	DT
cord-014597-66vd2mdu	487	25	size	size	NN
cord-014597-66vd2mdu	487	26	of	of	IN
cord-014597-66vd2mdu	487	27	ambr	ambr	NNP
cord-014597-66vd2mdu	487	28	®	®	NNP
cord-014597-66vd2mdu	487	29	15	15	CD
cord-014597-66vd2mdu	487	30	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	487	31	.	.	.
cord-014597-66vd2mdu	488	1	Moreover	moreover	RB
cord-014597-66vd2mdu	488	2	,	,	,
cord-014597-66vd2mdu	488	3	the	the	DT
cord-014597-66vd2mdu	488	4	applied	apply	VBN
cord-014597-66vd2mdu	488	5	methods	method	NNS
cord-014597-66vd2mdu	488	6	are	be	VBP
cord-014597-66vd2mdu	488	7	relatively	relatively	RB
cord-014597-66vd2mdu	488	8	low	low	JJ
cord-014597-66vd2mdu	488	9	throughput	throughput	NN
cord-014597-66vd2mdu	488	10	.	.	.
cord-014597-66vd2mdu	489	1	The	the	DT
cord-014597-66vd2mdu	489	2	development	development	NN
cord-014597-66vd2mdu	489	3	of	of	IN
cord-014597-66vd2mdu	489	4	a	a	DT
cord-014597-66vd2mdu	489	5	high	high	JJ
cord-014597-66vd2mdu	489	6	binding	binding	NN
cord-014597-66vd2mdu	489	7	capacity	capacity	NN
cord-014597-66vd2mdu	489	8	resin	resin	NN
cord-014597-66vd2mdu	489	9	(	(	-LRB-
cord-014597-66vd2mdu	489	10	up	up	IN
cord-014597-66vd2mdu	489	11	to	to	TO
cord-014597-66vd2mdu	489	12	70	70	CD
cord-014597-66vd2mdu	489	13	mg	mg	NN
cord-014597-66vd2mdu	489	14	/	/	SYM
cord-014597-66vd2mdu	489	15	mL	ml	NN
cord-014597-66vd2mdu	489	16	)	)	-RRB-
cord-014597-66vd2mdu	489	17	[	[	-LRB-
cord-014597-66vd2mdu	489	18	2	2	CD
cord-014597-66vd2mdu	489	19	]	]	-RRB-
cord-014597-66vd2mdu	489	20	,	,	,
cord-014597-66vd2mdu	489	21	combined	combine	VBN
cord-014597-66vd2mdu	489	22	with	with	IN
cord-014597-66vd2mdu	489	23	high	high	JJ
cord-014597-66vd2mdu	489	24	performing	perform	VBG
cord-014597-66vd2mdu	489	25	new	new	JJ
cord-014597-66vd2mdu	489	26	cell	cell	NN
cord-014597-66vd2mdu	489	27	lines	line	NNS
cord-014597-66vd2mdu	489	28	which	which	WDT
cord-014597-66vd2mdu	489	29	are	be	VBP
cord-014597-66vd2mdu	489	30	able	able	JJ
cord-014597-66vd2mdu	489	31	to	to	TO
cord-014597-66vd2mdu	489	32	produce	produce	VB
cord-014597-66vd2mdu	489	33	up	up	RP
cord-014597-66vd2mdu	489	34	to	to	TO
cord-014597-66vd2mdu	489	35	5	5	CD
cord-014597-66vd2mdu	489	36	g	g	NN
cord-014597-66vd2mdu	489	37	/	/	SYM
cord-014597-66vd2mdu	489	38	L	l	NN
cord-014597-66vd2mdu	489	39	of	of	IN
cord-014597-66vd2mdu	489	40	recombinant	recombinant	JJ
cord-014597-66vd2mdu	489	41	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	489	42	antibodies	antibody	NNS
cord-014597-66vd2mdu	489	43	,	,	,
cord-014597-66vd2mdu	489	44	allow	allow	VBP
cord-014597-66vd2mdu	489	45	require	require	VB
cord-014597-66vd2mdu	489	46	the	the	DT
cord-014597-66vd2mdu	489	47	development	development	NN
cord-014597-66vd2mdu	489	48	of	of	IN
cord-014597-66vd2mdu	489	49	an	an	DT
cord-014597-66vd2mdu	489	50	efficient	efficient	JJ
cord-014597-66vd2mdu	489	51	and	and	CC
cord-014597-66vd2mdu	489	52	high	high	JJ
cord-014597-66vd2mdu	489	53	throughput	throughput	NN
cord-014597-66vd2mdu	489	54	(	(	-LRB-
cord-014597-66vd2mdu	489	55	HTS	HTS	NNP
cord-014597-66vd2mdu	489	56	)	)	-RRB-
cord-014597-66vd2mdu	489	57	purification	purification	NN
cord-014597-66vd2mdu	489	58	method	method	NN
cord-014597-66vd2mdu	489	59	robot	robot	NN
cord-014597-66vd2mdu	489	60	.	.	.
cord-014597-66vd2mdu	490	1	The	the	DT
cord-014597-66vd2mdu	490	2	use	use	NN
cord-014597-66vd2mdu	490	3	of	of	IN
cord-014597-66vd2mdu	490	4	robotic	robotic	JJ
cord-014597-66vd2mdu	490	5	equipment	equipment	NN
cord-014597-66vd2mdu	490	6	for	for	IN
cord-014597-66vd2mdu	490	7	small	small	JJ
cord-014597-66vd2mdu	490	8	scale	scale	NN
cord-014597-66vd2mdu	490	9	purification	purification	NN
cord-014597-66vd2mdu	490	10	purposes	purpose	NNS
cord-014597-66vd2mdu	490	11	is	be	VBZ
cord-014597-66vd2mdu	490	12	a	a	DT
cord-014597-66vd2mdu	490	13	great	great	JJ
cord-014597-66vd2mdu	490	14	opportunity	opportunity	NN
cord-014597-66vd2mdu	490	15	for	for	IN
cord-014597-66vd2mdu	490	16	us	-PRON-	PRP
cord-014597-66vd2mdu	490	17	to	to	TO
cord-014597-66vd2mdu	490	18	tackle	tackle	VB
cord-014597-66vd2mdu	490	19	this	this	DT
cord-014597-66vd2mdu	490	20	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	490	21	,	,	,
cord-014597-66vd2mdu	490	22	by	by	IN
cord-014597-66vd2mdu	490	23	enabling	enable	VBG
cord-014597-66vd2mdu	490	24	highthroughput	highthroughput	NN
cord-014597-66vd2mdu	490	25	sample	sample	NN
cord-014597-66vd2mdu	490	26	purification	purification	NN
cord-014597-66vd2mdu	490	27	at	at	IN
cord-014597-66vd2mdu	490	28	smaller	small	JJR
cord-014597-66vd2mdu	490	29	scale	scale	NN
cord-014597-66vd2mdu	490	30	(	(	-LRB-
cord-014597-66vd2mdu	490	31	200μL	200μl	CD
cord-014597-66vd2mdu	490	32	)	)	-RRB-
cord-014597-66vd2mdu	490	33	.	.	.
cord-014597-66vd2mdu	491	1	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	491	2	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	491	3	antibodies	antibody	NNS
cord-014597-66vd2mdu	491	4	were	be	VBD
cord-014597-66vd2mdu	491	5	produced	produce	VBN
cord-014597-66vd2mdu	491	6	by	by	IN
cord-014597-66vd2mdu	491	7	a	a	DT
cord-014597-66vd2mdu	491	8	genetically	genetically	RB
cord-014597-66vd2mdu	491	9	engineered	engineer	VBN
cord-014597-66vd2mdu	491	10	Dihydrofolate	Dihydrofolate	NNP
cord-014597-66vd2mdu	491	11	Reductase	Reductase	NNP
cord-014597-66vd2mdu	491	12	(	(	-LRB-
cord-014597-66vd2mdu	491	13	DHFR)-/-DG44	dhfr)-/-dg44	JJ
cord-014597-66vd2mdu	491	14	Chinese	chinese	JJ
cord-014597-66vd2mdu	491	15	Hamster	Hamster	NNP
cord-014597-66vd2mdu	491	16	Ovary	Ovary	NNP
cord-014597-66vd2mdu	491	17	(	(	-LRB-
cord-014597-66vd2mdu	491	18	CHO	CHO	NNP
cord-014597-66vd2mdu	491	19	)	)	-RRB-
cord-014597-66vd2mdu	491	20	cell	cell	NN
cord-014597-66vd2mdu	491	21	line	line	NN
cord-014597-66vd2mdu	491	22	.	.	.
cord-014597-66vd2mdu	492	1	Clarified	clarify	VBN
cord-014597-66vd2mdu	492	2	cell	cell	NN
cord-014597-66vd2mdu	492	3	culture	culture	NN
cord-014597-66vd2mdu	492	4	fluid	fluid	NN
cord-014597-66vd2mdu	492	5	(	(	-LRB-
cord-014597-66vd2mdu	492	6	CCCF	CCCF	NNP
cord-014597-66vd2mdu	492	7	)	)	-RRB-
cord-014597-66vd2mdu	492	8	was	be	VBD
cord-014597-66vd2mdu	492	9	obtained	obtain	VBN
cord-014597-66vd2mdu	492	10	from	from	IN
cord-014597-66vd2mdu	492	11	2	2	CD
cord-014597-66vd2mdu	492	12	and	and	CC
cord-014597-66vd2mdu	492	13	2	2	CD
cord-014597-66vd2mdu	492	14	K	k	NN
cord-014597-66vd2mdu	492	15	liter	liter	NN
cord-014597-66vd2mdu	492	16	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	492	17	after	after	IN
cord-014597-66vd2mdu	492	18	three	three	CD
cord-014597-66vd2mdu	492	19	filtration	filtration	NN
cord-014597-66vd2mdu	492	20	steps	step	NNS
cord-014597-66vd2mdu	492	21	.	.	.
cord-014597-66vd2mdu	493	1	Minipurifications	minipurification	NNS
cord-014597-66vd2mdu	493	2	were	be	VBD
cord-014597-66vd2mdu	493	3	performed	perform	VBN
cord-014597-66vd2mdu	493	4	on	on	IN
cord-014597-66vd2mdu	494	1	Tecan	Tecan	NNP
cord-014597-66vd2mdu	494	2	Freedom	Freedom	NNP
cord-014597-66vd2mdu	494	3	EVO	EVO	NNP
cord-014597-66vd2mdu	494	4	®	®	NN
cord-014597-66vd2mdu	494	5	robot	robot	NN
cord-014597-66vd2mdu	494	6	with	with	IN
cord-014597-66vd2mdu	494	7	PreDictor	PreDictor	NNP
cord-014597-66vd2mdu	494	8	RoboCol	RoboCol	NNP
cord-014597-66vd2mdu	494	9	-	-	HYPH
cord-014597-66vd2mdu	494	10	umns	umns	NNP
cord-014597-66vd2mdu	495	1	®	®	LS
cord-014597-66vd2mdu	495	2	containing	contain	VBG
cord-014597-66vd2mdu	495	3	200μL	200μl	CD
cord-014597-66vd2mdu	495	4	Mabselect	Mabselect	NNP
cord-014597-66vd2mdu	495	5	SuRe	SuRe	NNP
cord-014597-66vd2mdu	495	6	®	®	POS
cord-014597-66vd2mdu	495	7	resin	resin	NN
cord-014597-66vd2mdu	495	8	.	.	.
cord-014597-66vd2mdu	496	1	Larger	large	JJR
cord-014597-66vd2mdu	496	2	scale	scale	NN
cord-014597-66vd2mdu	496	3	purification	purification	NN
cord-014597-66vd2mdu	496	4	were	be	VBD
cord-014597-66vd2mdu	496	5	executed	execute	VBN
cord-014597-66vd2mdu	496	6	using	use	VBG
cord-014597-66vd2mdu	496	7	an	an	DT
cord-014597-66vd2mdu	496	8	AktaXpress	AktaXpress	NNP
cord-014597-66vd2mdu	496	9	using	use	VBG
cord-014597-66vd2mdu	496	10	Hitrap	Hitrap	NNP
cord-014597-66vd2mdu	496	11	column	column	NN
cord-014597-66vd2mdu	496	12	ProtA.	ProtA.	NNS
cord-014597-66vd2mdu	496	13	To	to	TO
cord-014597-66vd2mdu	496	14	assess	assess	VB
cord-014597-66vd2mdu	496	15	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	496	16	antibody	antibody	NN
cord-014597-66vd2mdu	496	17	purification	purification	NN
cord-014597-66vd2mdu	496	18	at	at	IN
cord-014597-66vd2mdu	496	19	small	small	JJ
cord-014597-66vd2mdu	496	20	scale	scale	NN
cord-014597-66vd2mdu	496	21	,	,	,
cord-014597-66vd2mdu	496	22	we	-PRON-	PRP
cord-014597-66vd2mdu	496	23	first	first	RB
cord-014597-66vd2mdu	496	24	tested	test	VBD
cord-014597-66vd2mdu	496	25	the	the	DT
cord-014597-66vd2mdu	496	26	repeatability	repeatability	NN
cord-014597-66vd2mdu	496	27	of	of	IN
cord-014597-66vd2mdu	496	28	the	the	DT
cord-014597-66vd2mdu	496	29	minipurification	minipurification	NN
cord-014597-66vd2mdu	496	30	,	,	,
cord-014597-66vd2mdu	496	31	purifying	purify	VBG
cord-014597-66vd2mdu	496	32	the	the	DT
cord-014597-66vd2mdu	496	33	samples	sample	NNS
cord-014597-66vd2mdu	496	34	8	8	CD
cord-014597-66vd2mdu	496	35	times	time	NNS
cord-014597-66vd2mdu	496	36	on	on	IN
cord-014597-66vd2mdu	496	37	the	the	DT
cord-014597-66vd2mdu	496	38	same	same	JJ
cord-014597-66vd2mdu	496	39	columns	column	NNS
cord-014597-66vd2mdu	496	40	and	and	CC
cord-014597-66vd2mdu	496	41	using	use	VBG
cord-014597-66vd2mdu	496	42	different	different	JJ
cord-014597-66vd2mdu	496	43	columns	column	NNS
cord-014597-66vd2mdu	496	44	,	,	,
cord-014597-66vd2mdu	496	45	focusing	focus	VBG
cord-014597-66vd2mdu	496	46	on	on	IN
cord-014597-66vd2mdu	496	47	the	the	DT
cord-014597-66vd2mdu	496	48	yield	yield	NN
cord-014597-66vd2mdu	496	49	of	of	IN
cord-014597-66vd2mdu	496	50	the	the	DT
cord-014597-66vd2mdu	496	51	purification	purification	NN
cord-014597-66vd2mdu	496	52	and	and	CC
cord-014597-66vd2mdu	496	53	the	the	DT
cord-014597-66vd2mdu	496	54	impact	impact	NN
cord-014597-66vd2mdu	496	55	on	on	IN
cord-014597-66vd2mdu	496	56	Product	Product	NNP
cord-014597-66vd2mdu	496	57	Quality	Quality	NNP
cord-014597-66vd2mdu	496	58	Attributes	Attributes	NNPS
cord-014597-66vd2mdu	496	59	,	,	,
cord-014597-66vd2mdu	496	60	especially	especially	RB
cord-014597-66vd2mdu	496	61	the	the	DT
cord-014597-66vd2mdu	496	62	HMWS	HMWS	NNP
cord-014597-66vd2mdu	496	63	.	.	.
cord-014597-66vd2mdu	497	1	Then	then	RB
cord-014597-66vd2mdu	497	2	,	,	,
cord-014597-66vd2mdu	497	3	we	-PRON-	PRP
cord-014597-66vd2mdu	497	4	compared	compare	VBD
cord-014597-66vd2mdu	497	5	those	those	DT
cord-014597-66vd2mdu	497	6	results	result	NNS
cord-014597-66vd2mdu	497	7	to	to	IN
cord-014597-66vd2mdu	497	8	those	those	DT
cord-014597-66vd2mdu	497	9	obtained	obtain	VBN
cord-014597-66vd2mdu	497	10	with	with	IN
cord-014597-66vd2mdu	497	11	the	the	DT
cord-014597-66vd2mdu	497	12	AktaXpress	AktaXpress	NNP
cord-014597-66vd2mdu	497	13	at	at	IN
cord-014597-66vd2mdu	497	14	larger	large	JJR
cord-014597-66vd2mdu	497	15	scale	scale	NN
cord-014597-66vd2mdu	497	16	purification	purification	NN
cord-014597-66vd2mdu	497	17	,	,	,
cord-014597-66vd2mdu	497	18	comparing	compare	VBG
cord-014597-66vd2mdu	497	19	the	the	DT
cord-014597-66vd2mdu	497	20	yield	yield	NN
cord-014597-66vd2mdu	497	21	of	of	IN
cord-014597-66vd2mdu	497	22	the	the	DT
cord-014597-66vd2mdu	497	23	purification	purification	NN
cord-014597-66vd2mdu	497	24	and	and	CC
cord-014597-66vd2mdu	497	25	the	the	DT
cord-014597-66vd2mdu	497	26	PQA	pqa	NN
cord-014597-66vd2mdu	497	27	of	of	IN
cord-014597-66vd2mdu	497	28	the	the	DT
cord-014597-66vd2mdu	497	29	Protein	Protein	NNP
cord-014597-66vd2mdu	497	30	-	-	HYPH
cord-014597-66vd2mdu	497	31	A	A	NNP
cord-014597-66vd2mdu	497	32	eluates	eluate	NNS
cord-014597-66vd2mdu	497	33	obtained	obtain	VBN
cord-014597-66vd2mdu	497	34	with	with	IN
cord-014597-66vd2mdu	497	35	both	both	DT
cord-014597-66vd2mdu	497	36	purification	purification	NN
cord-014597-66vd2mdu	497	37	systems	system	NNS
cord-014597-66vd2mdu	497	38	.	.	.
cord-014597-66vd2mdu	498	1	Finally	finally	RB
cord-014597-66vd2mdu	498	2	,	,	,
cord-014597-66vd2mdu	498	3	we	-PRON-	PRP
cord-014597-66vd2mdu	498	4	assessed	assess	VBD
cord-014597-66vd2mdu	498	5	the	the	DT
cord-014597-66vd2mdu	498	6	capability	capability	NN
cord-014597-66vd2mdu	498	7	of	of	IN
cord-014597-66vd2mdu	498	8	the	the	DT
cord-014597-66vd2mdu	498	9	robot	robot	NN
cord-014597-66vd2mdu	498	10	to	to	TO
cord-014597-66vd2mdu	498	11	perform	perform	VB
cord-014597-66vd2mdu	498	12	HTS	HTS	NNP
cord-014597-66vd2mdu	498	13	of	of	IN
cord-014597-66vd2mdu	498	14	buffer	buffer	NN
cord-014597-66vd2mdu	498	15	and	and	CC
cord-014597-66vd2mdu	498	16	purification	purification	NN
cord-014597-66vd2mdu	498	17	conditions	condition	NNS
cord-014597-66vd2mdu	498	18	,	,	,
cord-014597-66vd2mdu	498	19	evaluating	evaluate	VBG
cord-014597-66vd2mdu	498	20	three	three	CD
cord-014597-66vd2mdu	498	21	different	different	JJ
cord-014597-66vd2mdu	498	22	buffers	buffer	NNS
cord-014597-66vd2mdu	498	23	at	at	IN
cord-014597-66vd2mdu	498	24	different	different	JJ
cord-014597-66vd2mdu	498	25	concentrations	concentration	NNS
cord-014597-66vd2mdu	498	26	and	and	CC
cord-014597-66vd2mdu	498	27	pH	ph	NN
cord-014597-66vd2mdu	498	28	values	value	NNS
cord-014597-66vd2mdu	498	29	,	,	,
cord-014597-66vd2mdu	498	30	and	and	CC
cord-014597-66vd2mdu	498	31	also	also	RB
cord-014597-66vd2mdu	498	32	testing	test	VBG
cord-014597-66vd2mdu	498	33	different	different	JJ
cord-014597-66vd2mdu	498	34	loading	loading	NN
cord-014597-66vd2mdu	498	35	column	column	NN
cord-014597-66vd2mdu	498	36	capacities	capacity	NNS
cord-014597-66vd2mdu	498	37	.	.	.
cord-014597-66vd2mdu	499	1	In	in	IN
cord-014597-66vd2mdu	499	2	this	this	DT
cord-014597-66vd2mdu	499	3	study	study	NN
cord-014597-66vd2mdu	499	4	we	-PRON-	PRP
cord-014597-66vd2mdu	499	5	established	establish	VBD
cord-014597-66vd2mdu	499	6	that	that	IN
cord-014597-66vd2mdu	499	7	the	the	DT
cord-014597-66vd2mdu	499	8	Tecan	Tecan	NNP
cord-014597-66vd2mdu	499	9	can	can	MD
cord-014597-66vd2mdu	499	10	be	be	VB
cord-014597-66vd2mdu	499	11	used	use	VBN
cord-014597-66vd2mdu	499	12	as	as	IN
cord-014597-66vd2mdu	499	13	a	a	DT
cord-014597-66vd2mdu	499	14	robust	robust	JJ
cord-014597-66vd2mdu	499	15	platform	platform	NN
cord-014597-66vd2mdu	499	16	for	for	IN
cord-014597-66vd2mdu	499	17	purification	purification	NN
cord-014597-66vd2mdu	499	18	at	at	IN
cord-014597-66vd2mdu	499	19	small	small	JJ
cord-014597-66vd2mdu	499	20	scale	scale	NN
cord-014597-66vd2mdu	499	21	.	.	.
cord-014597-66vd2mdu	500	1	We	-PRON-	PRP
cord-014597-66vd2mdu	500	2	observed	observe	VBD
cord-014597-66vd2mdu	500	3	similar	similar	JJ
cord-014597-66vd2mdu	500	4	purification	purification	NN
cord-014597-66vd2mdu	500	5	yields	yield	NNS
cord-014597-66vd2mdu	500	6	,	,	,
cord-014597-66vd2mdu	500	7	intra	intra	NNP
cord-014597-66vd2mdu	500	8	and	and	CC
cord-014597-66vd2mdu	500	9	inter	inter	JJ
cord-014597-66vd2mdu	500	10	run	run	NN
cord-014597-66vd2mdu	500	11	.	.	.
cord-014597-66vd2mdu	501	1	The	the	DT
cord-014597-66vd2mdu	501	2	analysis	analysis	NN
cord-014597-66vd2mdu	501	3	of	of	IN
cord-014597-66vd2mdu	501	4	the	the	DT
cord-014597-66vd2mdu	501	5	PQA1a	PQA1a	NNP
cord-014597-66vd2mdu	501	6	level	level	NN
cord-014597-66vd2mdu	501	7	showed	show	VBD
cord-014597-66vd2mdu	501	8	there	there	EX
cord-014597-66vd2mdu	501	9	is	be	VBZ
cord-014597-66vd2mdu	501	10	also	also	RB
cord-014597-66vd2mdu	501	11	very	very	RB
cord-014597-66vd2mdu	501	12	high	high	JJ
cord-014597-66vd2mdu	501	13	reproducibility	reproducibility	NN
cord-014597-66vd2mdu	501	14	.	.	.
cord-014597-66vd2mdu	502	1	And	and	CC
cord-014597-66vd2mdu	502	2	the	the	DT
cord-014597-66vd2mdu	502	3	pH	ph	NN
cord-014597-66vd2mdu	502	4	of	of	IN
cord-014597-66vd2mdu	502	5	the	the	DT
cord-014597-66vd2mdu	502	6	eluate	eluate	NN
cord-014597-66vd2mdu	502	7	showed	show	VBD
cord-014597-66vd2mdu	502	8	as	as	RB
cord-014597-66vd2mdu	502	9	well	well	RB
cord-014597-66vd2mdu	502	10	strong	strong	JJ
cord-014597-66vd2mdu	502	11	comparability	comparability	NN
cord-014597-66vd2mdu	502	12	as	as	RB
cord-014597-66vd2mdu	502	13	well	well	RB
cord-014597-66vd2mdu	502	14	.	.	.
cord-014597-66vd2mdu	503	1	Table	table	NN
cord-014597-66vd2mdu	503	2	1	1	CD
cord-014597-66vd2mdu	503	3	shows	show	VBZ
cord-014597-66vd2mdu	503	4	the	the	DT
cord-014597-66vd2mdu	503	5	coefficient	coefficient	NN
cord-014597-66vd2mdu	503	6	of	of	IN
cord-014597-66vd2mdu	503	7	variation	variation	NN
cord-014597-66vd2mdu	503	8	(	(	-LRB-
cord-014597-66vd2mdu	503	9	CV	CV	NNP
cord-014597-66vd2mdu	503	10	)	)	-RRB-
cord-014597-66vd2mdu	503	11	of	of	IN
cord-014597-66vd2mdu	503	12	the	the	DT
cord-014597-66vd2mdu	503	13	yield	yield	NN
cord-014597-66vd2mdu	503	14	,	,	,
cord-014597-66vd2mdu	504	1	HMWS	HMWS	NNP
cord-014597-66vd2mdu	504	2	and	and	CC
cord-014597-66vd2mdu	504	3	eluate	eluate	NNP
cord-014597-66vd2mdu	504	4	pH	pH	NNP
cord-014597-66vd2mdu	504	5	are	be	VBP
cord-014597-66vd2mdu	504	6	low	low	JJ
cord-014597-66vd2mdu	504	7	,	,	,
cord-014597-66vd2mdu	504	8	demonstrating	demonstrate	VBG
cord-014597-66vd2mdu	504	9	the	the	DT
cord-014597-66vd2mdu	504	10	good	good	JJ
cord-014597-66vd2mdu	504	11	reproducibility	reproducibility	NN
cord-014597-66vd2mdu	504	12	of	of	IN
cord-014597-66vd2mdu	504	13	the	the	DT
cord-014597-66vd2mdu	504	14	purification	purification	NN
cord-014597-66vd2mdu	504	15	.	.	.
cord-014597-66vd2mdu	505	1	The	the	DT
cord-014597-66vd2mdu	505	2	strong	strong	JJ
cord-014597-66vd2mdu	505	3	reproducibility	reproducibility	NN
cord-014597-66vd2mdu	505	4	obtained	obtain	VBN
cord-014597-66vd2mdu	505	5	between	between	IN
cord-014597-66vd2mdu	505	6	the	the	DT
cord-014597-66vd2mdu	505	7	different	different	JJ
cord-014597-66vd2mdu	505	8	purifications	purification	NNS
cord-014597-66vd2mdu	505	9	showed	show	VBD
cord-014597-66vd2mdu	505	10	that	that	IN
cord-014597-66vd2mdu	505	11	the	the	DT
cord-014597-66vd2mdu	505	12	Tecan	Tecan	NNP
cord-014597-66vd2mdu	505	13	and	and	CC
cord-014597-66vd2mdu	505	14	the	the	DT
cord-014597-66vd2mdu	505	15	AktaXpress	AktaXpress	NNP
cord-014597-66vd2mdu	505	16	are	be	VBP
cord-014597-66vd2mdu	505	17	similar	similar	JJ
cord-014597-66vd2mdu	505	18	in	in	IN
cord-014597-66vd2mdu	505	19	terms	term	NNS
cord-014597-66vd2mdu	505	20	of	of	IN
cord-014597-66vd2mdu	505	21	purification	purification	NN
cord-014597-66vd2mdu	505	22	performance	performance	NN
cord-014597-66vd2mdu	505	23	and	and	CC
cord-014597-66vd2mdu	505	24	PQA	PQA	NNP
cord-014597-66vd2mdu	505	25	(	(	-LRB-
cord-014597-66vd2mdu	505	26	Fig	Fig	NNP
cord-014597-66vd2mdu	505	27	.	.	.
cord-014597-66vd2mdu	505	28	1a	1a	CD
cord-014597-66vd2mdu	505	29	,	,	,
cord-014597-66vd2mdu	505	30	b	b	LS
cord-014597-66vd2mdu	505	31	)	)	-RRB-
cord-014597-66vd2mdu	505	32	.	.	.
cord-014597-66vd2mdu	506	1	The	the	DT
cord-014597-66vd2mdu	506	2	Tecan	Tecan	NNP
cord-014597-66vd2mdu	506	3	is	be	VBZ
cord-014597-66vd2mdu	506	4	a	a	DT
cord-014597-66vd2mdu	506	5	versatile	versatile	JJ
cord-014597-66vd2mdu	506	6	automated	automate	VBN
cord-014597-66vd2mdu	506	7	liquid	liquid	NN
cord-014597-66vd2mdu	506	8	handler	handler	NN
cord-014597-66vd2mdu	506	9	allowing	allow	VBG
cord-014597-66vd2mdu	506	10	the	the	DT
cord-014597-66vd2mdu	506	11	screening	screening	NN
cord-014597-66vd2mdu	506	12	of	of	IN
cord-014597-66vd2mdu	506	13	huge	huge	JJ
cord-014597-66vd2mdu	506	14	purification	purification	NN
cord-014597-66vd2mdu	506	15	conditions	condition	NNS
cord-014597-66vd2mdu	506	16	(	(	-LRB-
cord-014597-66vd2mdu	506	17	Fig	Fig	NNP
cord-014597-66vd2mdu	506	18	.	.	.
cord-014597-66vd2mdu	506	19	1c	1c	NNP
cord-014597-66vd2mdu	506	20	)	)	-RRB-
cord-014597-66vd2mdu	506	21	,	,	,
cord-014597-66vd2mdu	506	22	the	the	DT
cord-014597-66vd2mdu	506	23	possibility	possibility	NN
cord-014597-66vd2mdu	506	24	to	to	TO
cord-014597-66vd2mdu	506	25	purify	purify	VB
cord-014597-66vd2mdu	506	26	large	large	JJ
cord-014597-66vd2mdu	506	27	quantities	quantity	NNS
cord-014597-66vd2mdu	506	28	of	of	IN
cord-014597-66vd2mdu	506	29	samples	sample	NNS
cord-014597-66vd2mdu	506	30	,	,	,
cord-014597-66vd2mdu	506	31	while	while	IN
cord-014597-66vd2mdu	506	32	the	the	DT
cord-014597-66vd2mdu	506	33	samples	sample	NNS
cord-014597-66vd2mdu	506	34	amount	amount	NN
cord-014597-66vd2mdu	506	35	is	be	VBZ
cord-014597-66vd2mdu	506	36	limited	limit	VBN
cord-014597-66vd2mdu	506	37	.	.	.
cord-014597-66vd2mdu	507	1	The	the	DT
cord-014597-66vd2mdu	507	2	Tecan	Tecan	NNP
cord-014597-66vd2mdu	507	3	has	have	VBZ
cord-014597-66vd2mdu	507	4	the	the	DT
cord-014597-66vd2mdu	507	5	potential	potential	NN
cord-014597-66vd2mdu	507	6	to	to	TO
cord-014597-66vd2mdu	507	7	purify	purify	VB
cord-014597-66vd2mdu	507	8	more	more	JJR
cord-014597-66vd2mdu	507	9	than	than	IN
cord-014597-66vd2mdu	507	10	150	150	CD
cord-014597-66vd2mdu	507	11	samples	sample	NNS
cord-014597-66vd2mdu	507	12	/	/	SYM
cord-014597-66vd2mdu	507	13	day	day	NN
cord-014597-66vd2mdu	507	14	,	,	,
cord-014597-66vd2mdu	507	15	reducing	reduce	VBG
cord-014597-66vd2mdu	507	16	timelines	timeline	NNS
cord-014597-66vd2mdu	507	17	and	and	CC
cord-014597-66vd2mdu	507	18	allowing	allow	VBG
cord-014597-66vd2mdu	507	19	us	-PRON-	PRP
cord-014597-66vd2mdu	507	20	to	to	TO
cord-014597-66vd2mdu	507	21	deliver	deliver	VB
cord-014597-66vd2mdu	507	22	faster	fast	RBR
cord-014597-66vd2mdu	507	23	to	to	IN
cord-014597-66vd2mdu	507	24	the	the	DT
cord-014597-66vd2mdu	507	25	patients	patient	NNS
cord-014597-66vd2mdu	507	26	.	.	.
cord-014597-66vd2mdu	508	1	Viable	viable	JJ
cord-014597-66vd2mdu	508	2	cell	cell	NN
cord-014597-66vd2mdu	508	3	density	density	NN
cord-014597-66vd2mdu	508	4	monitoring	monitoring	NN
cord-014597-66vd2mdu	508	5	in	in	IN
cord-014597-66vd2mdu	508	6	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	508	7	with	with	IN
cord-014597-66vd2mdu	508	8	lensless	lensless	NN
cord-014597-66vd2mdu	508	9	imaging	imaging	NN
cord-014597-66vd2mdu	508	10	Geoffrey	Geoffrey	NNP
cord-014597-66vd2mdu	508	11	Monitoring	Monitoring	NNP
cord-014597-66vd2mdu	508	12	cell	cell	NN
cord-014597-66vd2mdu	508	13	density	density	NN
cord-014597-66vd2mdu	508	14	and	and	CC
cord-014597-66vd2mdu	508	15	viability	viability	NN
cord-014597-66vd2mdu	508	16	of	of	IN
cord-014597-66vd2mdu	508	17	mammalian	mammalian	JJ
cord-014597-66vd2mdu	508	18	cell	cell	NN
cord-014597-66vd2mdu	508	19	culture	culture	NN
cord-014597-66vd2mdu	508	20	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	508	21	is	be	VBZ
cord-014597-66vd2mdu	508	22	a	a	DT
cord-014597-66vd2mdu	508	23	necessary	necessary	JJ
cord-014597-66vd2mdu	508	24	task	task	NN
cord-014597-66vd2mdu	508	25	that	that	WDT
cord-014597-66vd2mdu	508	26	presents	present	VBZ
cord-014597-66vd2mdu	508	27	today	today	NN
cord-014597-66vd2mdu	508	28	a	a	DT
cord-014597-66vd2mdu	508	29	number	number	NN
cord-014597-66vd2mdu	508	30	of	of	IN
cord-014597-66vd2mdu	508	31	remaining	remain	VBG
cord-014597-66vd2mdu	508	32	challenges	challenge	NNS
cord-014597-66vd2mdu	508	33	.	.	.
cord-014597-66vd2mdu	509	1	The	the	DT
cord-014597-66vd2mdu	509	2	traditional	traditional	JJ
cord-014597-66vd2mdu	509	3	measurement	measurement	NN
cord-014597-66vd2mdu	509	4	for	for	IN
cord-014597-66vd2mdu	509	5	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	509	6	cell	cell	NN
cord-014597-66vd2mdu	509	7	count	count	NN
cord-014597-66vd2mdu	509	8	and	and	CC
cord-014597-66vd2mdu	509	9	viability	viability	NN
cord-014597-66vd2mdu	509	10	rely	rely	VBP
cord-014597-66vd2mdu	509	11	on	on	IN
cord-014597-66vd2mdu	509	12	using	use	VBG
cord-014597-66vd2mdu	509	13	the	the	DT
cord-014597-66vd2mdu	509	14	Trypan	Trypan	NNP
cord-014597-66vd2mdu	509	15	Blue	Blue	NNP
cord-014597-66vd2mdu	509	16	exclusion	exclusion	NN
cord-014597-66vd2mdu	509	17	method	method	NN
cord-014597-66vd2mdu	509	18	once	once	RB
cord-014597-66vd2mdu	509	19	a	a	DT
cord-014597-66vd2mdu	509	20	day	day	NN
cord-014597-66vd2mdu	509	21	.	.	.
cord-014597-66vd2mdu	510	1	While	while	IN
cord-014597-66vd2mdu	510	2	automatic	automatic	JJ
cord-014597-66vd2mdu	510	3	cell	cell	NN
cord-014597-66vd2mdu	510	4	counters	counter	NNS
cord-014597-66vd2mdu	510	5	have	have	VBP
cord-014597-66vd2mdu	510	6	reduced	reduce	VBN
cord-014597-66vd2mdu	510	7	the	the	DT
cord-014597-66vd2mdu	510	8	statistical	statistical	JJ
cord-014597-66vd2mdu	510	9	manual	manual	JJ
cord-014597-66vd2mdu	510	10	error	error	NN
cord-014597-66vd2mdu	510	11	,	,	,
cord-014597-66vd2mdu	510	12	sampling	sample	VBG
cord-014597-66vd2mdu	510	13	the	the	DT
cord-014597-66vd2mdu	510	14	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	510	15	remains	remain	VBZ
cord-014597-66vd2mdu	510	16	a	a	DT
cord-014597-66vd2mdu	510	17	contamination	contamination	NN
cord-014597-66vd2mdu	510	18	risk	risk	NN
cord-014597-66vd2mdu	510	19	and	and	CC
cord-014597-66vd2mdu	510	20	is	be	VBZ
cord-014597-66vd2mdu	510	21	prohibiting	prohibit	VBG
cord-014597-66vd2mdu	510	22	process	process	NN
cord-014597-66vd2mdu	510	23	control	control	NN
cord-014597-66vd2mdu	510	24	as	as	IN
cord-014597-66vd2mdu	510	25	the	the	DT
cord-014597-66vd2mdu	510	26	sampled	sample	VBN
cord-014597-66vd2mdu	510	27	volume	volume	NN
cord-014597-66vd2mdu	510	28	becomes	become	VBZ
cord-014597-66vd2mdu	510	29	siginficant	siginficant	JJ
cord-014597-66vd2mdu	510	30	.	.	.
cord-014597-66vd2mdu	511	1	Lensless	Lensless	NNP
cord-014597-66vd2mdu	511	2	Imaging	Imaging	NNP
cord-014597-66vd2mdu	511	3	Technology	Technology	NNP
cord-014597-66vd2mdu	511	4	is	be	VBZ
cord-014597-66vd2mdu	511	5	a	a	DT
cord-014597-66vd2mdu	511	6	new	new	JJ
cord-014597-66vd2mdu	511	7	method	method	NN
cord-014597-66vd2mdu	511	8	for	for	IN
cord-014597-66vd2mdu	511	9	accurately	accurately	RB
cord-014597-66vd2mdu	511	10	determining	determine	VBG
cord-014597-66vd2mdu	511	11	cell	cell	NN
cord-014597-66vd2mdu	511	12	concentration	concentration	NN
cord-014597-66vd2mdu	511	13	and	and	CC
cord-014597-66vd2mdu	511	14	viability	viability	NN
cord-014597-66vd2mdu	511	15	without	without	IN
cord-014597-66vd2mdu	511	16	staining	stain	VBG
cord-014597-66vd2mdu	511	17	.	.	.
cord-014597-66vd2mdu	512	1	This	this	DT
cord-014597-66vd2mdu	512	2	technique	technique	NN
cord-014597-66vd2mdu	512	3	directly	directly	RB
cord-014597-66vd2mdu	512	4	acquires	acquire	VBZ
cord-014597-66vd2mdu	512	5	the	the	DT
cord-014597-66vd2mdu	512	6	light	light	JJ
cord-014597-66vd2mdu	512	7	diffraction	diffraction	NN
cord-014597-66vd2mdu	512	8	properties	property	NNS
cord-014597-66vd2mdu	512	9	of	of	IN
cord-014597-66vd2mdu	512	10	each	each	DT
cord-014597-66vd2mdu	512	11	individual	individual	JJ
cord-014597-66vd2mdu	512	12	cells	cell	VBZ
cord-014597-66vd2mdu	512	13	through	through	IN
cord-014597-66vd2mdu	512	14	their	-PRON-	PRP$
cord-014597-66vd2mdu	512	15	holograms	hologram	NNS
cord-014597-66vd2mdu	512	16	images	image	NNS
cord-014597-66vd2mdu	512	17	without	without	IN
cord-014597-66vd2mdu	512	18	any	any	DT
cord-014597-66vd2mdu	512	19	objective	objective	NN
cord-014597-66vd2mdu	512	20	,	,	,
cord-014597-66vd2mdu	512	21	lens	lens	NN
cord-014597-66vd2mdu	512	22	or	or	CC
cord-014597-66vd2mdu	512	23	focus	focus	VB
cord-014597-66vd2mdu	512	24	settings	setting	NNS
cord-014597-66vd2mdu	512	25	.	.	.
cord-014597-66vd2mdu	513	1	Living	live	VBG
cord-014597-66vd2mdu	513	2	and	and	CC
cord-014597-66vd2mdu	513	3	dead	dead	JJ
cord-014597-66vd2mdu	513	4	cells	cell	NNS
cord-014597-66vd2mdu	513	5	have	have	VBP
cord-014597-66vd2mdu	513	6	significant	significant	JJ
cord-014597-66vd2mdu	513	7	holographic	holographic	JJ
cord-014597-66vd2mdu	513	8	patterns	pattern	NNS
cord-014597-66vd2mdu	513	9	that	that	WDT
cord-014597-66vd2mdu	513	10	can	can	MD
cord-014597-66vd2mdu	513	11	be	be	VB
cord-014597-66vd2mdu	513	12	distinguished	distinguished	JJ
cord-014597-66vd2mdu	513	13	and	and	CC
cord-014597-66vd2mdu	513	14	precisely	precisely	RB
cord-014597-66vd2mdu	513	15	counted	count	VBN
cord-014597-66vd2mdu	513	16	.	.	.
cord-014597-66vd2mdu	514	1	Lensless	lensless	NN
cord-014597-66vd2mdu	514	2	imaging	imaging	NN
cord-014597-66vd2mdu	514	3	technique	technique	NN
cord-014597-66vd2mdu	514	4	directly	directly	RB
cord-014597-66vd2mdu	514	5	acquires	acquire	VBZ
cord-014597-66vd2mdu	514	6	the	the	DT
cord-014597-66vd2mdu	514	7	light	light	JJ
cord-014597-66vd2mdu	514	8	diffraction	diffraction	NN
cord-014597-66vd2mdu	514	9	properties	property	NNS
cord-014597-66vd2mdu	514	10	of	of	IN
cord-014597-66vd2mdu	514	11	each	each	DT
cord-014597-66vd2mdu	514	12	individual	individual	JJ
cord-014597-66vd2mdu	514	13	cells	cell	VBZ
cord-014597-66vd2mdu	514	14	through	through	IN
cord-014597-66vd2mdu	514	15	their	-PRON-	PRP$
cord-014597-66vd2mdu	514	16	holograms	hologram	NNS
cord-014597-66vd2mdu	514	17	images	image	NNS
cord-014597-66vd2mdu	514	18	without	without	IN
cord-014597-66vd2mdu	514	19	any	any	DT
cord-014597-66vd2mdu	514	20	objective	objective	NN
cord-014597-66vd2mdu	514	21	,	,	,
cord-014597-66vd2mdu	514	22	lens	lens	NN
cord-014597-66vd2mdu	514	23	or	or	CC
cord-014597-66vd2mdu	514	24	focus	focus	VB
cord-014597-66vd2mdu	514	25	settings	setting	NNS
cord-014597-66vd2mdu	514	26	.	.	.
cord-014597-66vd2mdu	515	1	Living	live	VBG
cord-014597-66vd2mdu	515	2	and	and	CC
cord-014597-66vd2mdu	515	3	dead	dead	JJ
cord-014597-66vd2mdu	515	4	cells	cell	NNS
cord-014597-66vd2mdu	515	5	have	have	VBP
cord-014597-66vd2mdu	515	6	significant	significant	JJ
cord-014597-66vd2mdu	515	7	holographic	holographic	JJ
cord-014597-66vd2mdu	515	8	patterns	pattern	NNS
cord-014597-66vd2mdu	515	9	that	that	WDT
cord-014597-66vd2mdu	515	10	can	can	MD
cord-014597-66vd2mdu	515	11	be	be	VB
cord-014597-66vd2mdu	515	12	distinguished	distinguished	JJ
cord-014597-66vd2mdu	515	13	and	and	CC
cord-014597-66vd2mdu	515	14	precisely	precisely	RB
cord-014597-66vd2mdu	515	15	counted	count	VBN
cord-014597-66vd2mdu	515	16	.	.	.
cord-014597-66vd2mdu	516	1	We	-PRON-	PRP
cord-014597-66vd2mdu	516	2	compare	compare	VBP
cord-014597-66vd2mdu	516	3	cell	cell	NN
cord-014597-66vd2mdu	516	4	counts	count	NNS
cord-014597-66vd2mdu	516	5	and	and	CC
cord-014597-66vd2mdu	516	6	viability	viability	NN
cord-014597-66vd2mdu	516	7	between	between	IN
cord-014597-66vd2mdu	516	8	the	the	DT
cord-014597-66vd2mdu	516	9	reference	reference	NN
cord-014597-66vd2mdu	516	10	method	method	NN
cord-014597-66vd2mdu	516	11	and	and	CC
cord-014597-66vd2mdu	516	12	our	-PRON-	PRP$
cord-014597-66vd2mdu	516	13	Lensless	Lensless	NNP
cord-014597-66vd2mdu	516	14	Imaging	Imaging	NNP
cord-014597-66vd2mdu	516	15	device	device	NN
cord-014597-66vd2mdu	516	16	,	,	,
cord-014597-66vd2mdu	516	17	the	the	DT
cord-014597-66vd2mdu	516	18	Cytonote	Cytonote	NNP
cord-014597-66vd2mdu	516	19	counter	counter	NN
cord-014597-66vd2mdu	516	20	.	.	.
cord-014597-66vd2mdu	517	1	Measures	measure	NNS
cord-014597-66vd2mdu	517	2	are	be	VBP
cord-014597-66vd2mdu	517	3	performed	perform	VBN
cord-014597-66vd2mdu	517	4	once	once	RB
cord-014597-66vd2mdu	517	5	a	a	DT
cord-014597-66vd2mdu	517	6	day	day	NN
cord-014597-66vd2mdu	517	7	on	on	IN
cord-014597-66vd2mdu	517	8	samples	sample	NNS
cord-014597-66vd2mdu	517	9	from	from	IN
cord-014597-66vd2mdu	517	10	12	12	CD
cord-014597-66vd2mdu	517	11	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	517	12	,	,	,
cord-014597-66vd2mdu	517	13	from	from	IN
cord-014597-66vd2mdu	517	14	the	the	DT
cord-014597-66vd2mdu	517	15	inoculation	inoculation	NN
cord-014597-66vd2mdu	517	16	to	to	IN
cord-014597-66vd2mdu	517	17	the	the	DT
cord-014597-66vd2mdu	517	18	end	end	NN
cord-014597-66vd2mdu	517	19	of	of	IN
cord-014597-66vd2mdu	517	20	the	the	DT
cord-014597-66vd2mdu	517	21	culture	culture	NN
cord-014597-66vd2mdu	517	22	.	.	.
cord-014597-66vd2mdu	518	1	We	-PRON-	PRP
cord-014597-66vd2mdu	518	2	also	also	RB
cord-014597-66vd2mdu	518	3	assessed	assess	VBD
cord-014597-66vd2mdu	518	4	the	the	DT
cord-014597-66vd2mdu	518	5	repeatability	repeatability	NN
cord-014597-66vd2mdu	518	6	of	of	IN
cord-014597-66vd2mdu	518	7	our	-PRON-	PRP$
cord-014597-66vd2mdu	518	8	method	method	NN
cord-014597-66vd2mdu	518	9	.	.	.
cord-014597-66vd2mdu	519	1	Another	another	DT
cord-014597-66vd2mdu	519	2	Lensless	Lensless	NNP
cord-014597-66vd2mdu	519	3	Imaging	Imaging	NNP
cord-014597-66vd2mdu	519	4	prototype	prototype	NN
cord-014597-66vd2mdu	519	5	is	be	VBZ
cord-014597-66vd2mdu	519	6	setup	setup	NN
cord-014597-66vd2mdu	519	7	as	as	IN
cord-014597-66vd2mdu	519	8	a	a	DT
cord-014597-66vd2mdu	519	9	measurement	measurement	NN
cord-014597-66vd2mdu	519	10	chamber	chamber	NN
cord-014597-66vd2mdu	519	11	directly	directly	RB
cord-014597-66vd2mdu	519	12	connected	connect	VBN
cord-014597-66vd2mdu	519	13	to	to	IN
cord-014597-66vd2mdu	519	14	a	a	DT
cord-014597-66vd2mdu	519	15	perfusion	perfusion	NN
cord-014597-66vd2mdu	519	16	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	519	17	,	,	,
cord-014597-66vd2mdu	519	18	for	for	IN
cord-014597-66vd2mdu	519	19	continuously	continuously	RB
cord-014597-66vd2mdu	519	20	receiving	receive	VBG
cord-014597-66vd2mdu	519	21	the	the	DT
cord-014597-66vd2mdu	519	22	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	519	23	broth	broth	NN
cord-014597-66vd2mdu	519	24	,	,	,
cord-014597-66vd2mdu	519	25	and	and	CC
cord-014597-66vd2mdu	519	26	therefore	therefore	RB
cord-014597-66vd2mdu	519	27	reproducing	reproduce	VBG
cord-014597-66vd2mdu	519	28	an	an	DT
cord-014597-66vd2mdu	519	29	in	in	IN
cord-014597-66vd2mdu	519	30	situ	situ	JJ
cord-014597-66vd2mdu	519	31	measure	measure	NN
cord-014597-66vd2mdu	519	32	.	.	.
cord-014597-66vd2mdu	520	1	With	with	IN
cord-014597-66vd2mdu	520	2	a	a	DT
cord-014597-66vd2mdu	520	3	concentration	concentration	NN
cord-014597-66vd2mdu	520	4	range	range	NN
cord-014597-66vd2mdu	520	5	up	up	IN
cord-014597-66vd2mdu	520	6	to	to	IN
cord-014597-66vd2mdu	520	7	40x10	40x10	CD
cord-014597-66vd2mdu	520	8	6	6	CD
cord-014597-66vd2mdu	520	9	cells	cell	NNS
cord-014597-66vd2mdu	520	10	/	/	SYM
cord-014597-66vd2mdu	520	11	ml	ml	NN
cord-014597-66vd2mdu	521	1	(	(	-LRB-
cord-014597-66vd2mdu	521	2	Fig	Fig	NNP
cord-014597-66vd2mdu	521	3	.	.	NNP
cord-014597-66vd2mdu	521	4	1	1	CD
cord-014597-66vd2mdu	521	5	)	)	-RRB-
cord-014597-66vd2mdu	522	1	and	and	CC
cord-014597-66vd2mdu	522	2	viability	viability	NN
cord-014597-66vd2mdu	522	3	range	range	NN
cord-014597-66vd2mdu	522	4	at	at	IN
cord-014597-66vd2mdu	522	5	75	75	CD
cord-014597-66vd2mdu	522	6	-	-	SYM
cord-014597-66vd2mdu	522	7	100	100	CD
cord-014597-66vd2mdu	522	8	%	%	NN
cord-014597-66vd2mdu	523	1	,	,	,
cord-014597-66vd2mdu	523	2	we	-PRON-	PRP
cord-014597-66vd2mdu	523	3	obtained	obtain	VBD
cord-014597-66vd2mdu	523	4	a	a	DT
cord-014597-66vd2mdu	523	5	correlation	correlation	NN
cord-014597-66vd2mdu	523	6	factor	factor	NN
cord-014597-66vd2mdu	523	7	of	of	IN
cord-014597-66vd2mdu	523	8	0.98	0.98	CD
cord-014597-66vd2mdu	523	9	between	between	IN
cord-014597-66vd2mdu	523	10	the	the	DT
cord-014597-66vd2mdu	523	11	two	two	CD
cord-014597-66vd2mdu	523	12	compared	compare	VBN
cord-014597-66vd2mdu	523	13	methods	method	NNS
cord-014597-66vd2mdu	523	14	.	.	.
cord-014597-66vd2mdu	524	1	The	the	DT
cord-014597-66vd2mdu	524	2	large	large	JJ
cord-014597-66vd2mdu	524	3	field	field	NN
cord-014597-66vd2mdu	524	4	of	of	IN
cord-014597-66vd2mdu	524	5	view	view	NN
cord-014597-66vd2mdu	524	6	allows	allow	VBZ
cord-014597-66vd2mdu	524	7	the	the	DT
cord-014597-66vd2mdu	524	8	analyze	analyze	NN
cord-014597-66vd2mdu	524	9	of	of	IN
cord-014597-66vd2mdu	524	10	several	several	JJ
cord-014597-66vd2mdu	524	11	thousand	thousand	CD
cord-014597-66vd2mdu	524	12	cells	cell	NNS
cord-014597-66vd2mdu	524	13	within	within	IN
cord-014597-66vd2mdu	524	14	a	a	DT
cord-014597-66vd2mdu	524	15	single	single	JJ
cord-014597-66vd2mdu	524	16	image	image	NN
cord-014597-66vd2mdu	524	17	,	,	,
cord-014597-66vd2mdu	524	18	keeping	keep	VBG
cord-014597-66vd2mdu	524	19	the	the	DT
cord-014597-66vd2mdu	524	20	statistical	statistical	JJ
cord-014597-66vd2mdu	524	21	variability	variability	NN
cord-014597-66vd2mdu	524	22	of	of	IN
cord-014597-66vd2mdu	524	23	the	the	DT
cord-014597-66vd2mdu	524	24	measure	measure	NN
cord-014597-66vd2mdu	524	25	as	as	RB
cord-014597-66vd2mdu	524	26	low	low	JJ
cord-014597-66vd2mdu	524	27	as	as	IN
cord-014597-66vd2mdu	524	28	3	3	CD
cord-014597-66vd2mdu	524	29	%	%	NN
cord-014597-66vd2mdu	524	30	.	.	.
cord-014597-66vd2mdu	525	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	525	2	measurement	measurement	NN
cord-014597-66vd2mdu	525	3	chamber	chamber	NN
cord-014597-66vd2mdu	525	4	prototype	prototype	NN
cord-014597-66vd2mdu	525	5	has	have	VBZ
cord-014597-66vd2mdu	525	6	demonstarted	demonstarte	VBN
cord-014597-66vd2mdu	525	7	its	-PRON-	PRP$
cord-014597-66vd2mdu	525	8	capability	capability	NN
cord-014597-66vd2mdu	525	9	for	for	IN
cord-014597-66vd2mdu	525	10	continuous	continuous	JJ
cord-014597-66vd2mdu	525	11	Viable	viable	JJ
cord-014597-66vd2mdu	525	12	Cell	cell	NN
cord-014597-66vd2mdu	525	13	Density	density	NN
cord-014597-66vd2mdu	525	14	and	and	CC
cord-014597-66vd2mdu	525	15	viability	viability	NN
cord-014597-66vd2mdu	525	16	monitoring	monitoring	NN
cord-014597-66vd2mdu	525	17	.	.	.
cord-014597-66vd2mdu	526	1	We	-PRON-	PRP
cord-014597-66vd2mdu	526	2	are	be	VBP
cord-014597-66vd2mdu	526	3	now	now	RB
cord-014597-66vd2mdu	526	4	working	work	VBG
cord-014597-66vd2mdu	526	5	at	at	IN
cord-014597-66vd2mdu	526	6	designing	design	VBG
cord-014597-66vd2mdu	526	7	a	a	DT
cord-014597-66vd2mdu	526	8	steam	steam	NN
cord-014597-66vd2mdu	526	9	strerilizable	strerilizable	JJ
cord-014597-66vd2mdu	526	10	probe	probe	NN
cord-014597-66vd2mdu	526	11	,	,	,
cord-014597-66vd2mdu	526	12	and	and	CC
cord-014597-66vd2mdu	526	13	we	-PRON-	PRP
cord-014597-66vd2mdu	526	14	envision	envision	VBP
cord-014597-66vd2mdu	526	15	Lensless	Lensless	NNP
cord-014597-66vd2mdu	526	16	Imaging	Imaging	NNP
cord-014597-66vd2mdu	526	17	to	to	TO
cord-014597-66vd2mdu	526	18	become	become	VB
cord-014597-66vd2mdu	526	19	the	the	DT
cord-014597-66vd2mdu	526	20	future	future	JJ
cord-014597-66vd2mdu	526	21	method	method	NN
cord-014597-66vd2mdu	526	22	of	of	IN
cord-014597-66vd2mdu	526	23	choice	choice	NN
cord-014597-66vd2mdu	526	24	for	for	IN
cord-014597-66vd2mdu	526	25	on	on	IN
cord-014597-66vd2mdu	526	26	-	-	HYPH
cord-014597-66vd2mdu	526	27	line	line	NN
cord-014597-66vd2mdu	526	28	monitoring	monitoring	NN
cord-014597-66vd2mdu	526	29	of	of	IN
cord-014597-66vd2mdu	526	30	suspension	suspension	NN
cord-014597-66vd2mdu	526	31	cells	cell	NNS
cord-014597-66vd2mdu	526	32	cultures	culture	NNS
cord-014597-66vd2mdu	526	33	.	.	.
cord-014597-66vd2mdu	527	1	Lensless	Lensless	NNP
cord-014597-66vd2mdu	527	2	Imaging	Imaging	NNP
cord-014597-66vd2mdu	527	3	Technology	Technology	NNP
cord-014597-66vd2mdu	527	4	is	be	VBZ
cord-014597-66vd2mdu	527	5	capable	capable	JJ
cord-014597-66vd2mdu	527	6	of	of	IN
cord-014597-66vd2mdu	527	7	accurately	accurately	RB
cord-014597-66vd2mdu	527	8	and	and	CC
cord-014597-66vd2mdu	527	9	precisely	precisely	RB
cord-014597-66vd2mdu	527	10	monitoring	monitor	VBG
cord-014597-66vd2mdu	527	11	Viable	viable	JJ
cord-014597-66vd2mdu	527	12	Cell	cell	NN
cord-014597-66vd2mdu	527	13	Density	density	NN
cord-014597-66vd2mdu	527	14	and	and	CC
cord-014597-66vd2mdu	527	15	Viability	Viability	NNP
cord-014597-66vd2mdu	527	16	with	with	IN
cord-014597-66vd2mdu	527	17	a	a	DT
cord-014597-66vd2mdu	527	18	combination	combination	NN
cord-014597-66vd2mdu	527	19	of	of	IN
cord-014597-66vd2mdu	527	20	significant	significant	JJ
cord-014597-66vd2mdu	527	21	advantages	advantage	NNS
cord-014597-66vd2mdu	527	22	starting	start	VBG
cord-014597-66vd2mdu	527	23	from	from	IN
cord-014597-66vd2mdu	527	24	low	low	JJ
cord-014597-66vd2mdu	527	25	sample	sample	NN
cord-014597-66vd2mdu	527	26	volume	volume	NN
cord-014597-66vd2mdu	527	27	use	use	NN
cord-014597-66vd2mdu	527	28	,	,	,
cord-014597-66vd2mdu	527	29	label	label	VB
cord-014597-66vd2mdu	527	30	free	free	JJ
cord-014597-66vd2mdu	527	31	detection	detection	NN
cord-014597-66vd2mdu	527	32	,	,	,
cord-014597-66vd2mdu	527	33	quick	quick	JJ
cord-014597-66vd2mdu	527	34	measure	measure	NN
cord-014597-66vd2mdu	527	35	,	,	,
cord-014597-66vd2mdu	527	36	simple	simple	JJ
cord-014597-66vd2mdu	527	37	device	device	NN
cord-014597-66vd2mdu	527	38	,	,	,
cord-014597-66vd2mdu	527	39	to	to	IN
cord-014597-66vd2mdu	527	40	high	high	JJ
cord-014597-66vd2mdu	527	41	number	number	NN
cord-014597-66vd2mdu	527	42	of	of	IN
cord-014597-66vd2mdu	527	43	cell	cell	NN
cord-014597-66vd2mdu	527	44	analyzed	analyze	VBN
cord-014597-66vd2mdu	527	45	which	which	WDT
cord-014597-66vd2mdu	527	46	let	let	VBD
cord-014597-66vd2mdu	527	47	us	-PRON-	PRP
cord-014597-66vd2mdu	527	48	think	think	VB
cord-014597-66vd2mdu	527	49	that	that	IN
cord-014597-66vd2mdu	527	50	it	-PRON-	PRP
cord-014597-66vd2mdu	527	51	is	be	VBZ
cord-014597-66vd2mdu	527	52	a	a	DT
cord-014597-66vd2mdu	527	53	good	good	JJ
cord-014597-66vd2mdu	527	54	candidate	candidate	NN
cord-014597-66vd2mdu	527	55	for	for	IN
cord-014597-66vd2mdu	527	56	very	very	RB
cord-014597-66vd2mdu	527	57	small-	small-	JJ
cord-014597-66vd2mdu	528	1	Comparison	comparison	NN
cord-014597-66vd2mdu	528	2	between	between	IN
cord-014597-66vd2mdu	528	3	both	both	DT
cord-014597-66vd2mdu	528	4	purification	purification	NN
cord-014597-66vd2mdu	528	5	systems	system	NNS
cord-014597-66vd2mdu	528	6	and	and	CC
cord-014597-66vd2mdu	528	7	the	the	DT
cord-014597-66vd2mdu	528	8	ability	ability	NN
cord-014597-66vd2mdu	528	9	of	of	IN
cord-014597-66vd2mdu	528	10	the	the	DT
cord-014597-66vd2mdu	528	11	system	system	NN
cord-014597-66vd2mdu	528	12	to	to	TO
cord-014597-66vd2mdu	528	13	be	be	VB
cord-014597-66vd2mdu	528	14	used	use	VBN
cord-014597-66vd2mdu	528	15	as	as	IN
cord-014597-66vd2mdu	528	16	a	a	DT
cord-014597-66vd2mdu	528	17	high	high	JJ
cord-014597-66vd2mdu	528	18	throughput	throughput	NN
cord-014597-66vd2mdu	528	19	tools	tool	NNS
cord-014597-66vd2mdu	528	20	for	for	IN
cord-014597-66vd2mdu	528	21	buffers	buffer	NNS
cord-014597-66vd2mdu	528	22	screening	screen	VBG
cord-014597-66vd2mdu	528	23	.	.	.
cord-014597-66vd2mdu	529	1	a	a	DT
cord-014597-66vd2mdu	529	2	Purification	purification	NN
cord-014597-66vd2mdu	529	3	yield	yield	NN
cord-014597-66vd2mdu	529	4	(	(	-LRB-
cord-014597-66vd2mdu	529	5	%	%	NN
cord-014597-66vd2mdu	529	6	)	)	-RRB-
cord-014597-66vd2mdu	529	7	.	.	.
cord-014597-66vd2mdu	530	1	b	b	LS
cord-014597-66vd2mdu	530	2	PQA1.a	PQA1.a	NNP
cord-014597-66vd2mdu	530	3	(	(	-LRB-
cord-014597-66vd2mdu	530	4	normalized	normalize	VBN
cord-014597-66vd2mdu	530	5	)	)	-RRB-
cord-014597-66vd2mdu	530	6	.	.	.
cord-014597-66vd2mdu	531	1	c	c	LS
cord-014597-66vd2mdu	531	2	Impact	Impact	NNP
cord-014597-66vd2mdu	531	3	of	of	IN
cord-014597-66vd2mdu	531	4	the	the	DT
cord-014597-66vd2mdu	531	5	pH	ph	NN
cord-014597-66vd2mdu	531	6	and	and	CC
cord-014597-66vd2mdu	531	7	buffer	buffer	NN
cord-014597-66vd2mdu	531	8	concentration	concentration	NN
cord-014597-66vd2mdu	531	9	on	on	IN
cord-014597-66vd2mdu	531	10	the	the	DT
cord-014597-66vd2mdu	531	11	PQA1.a	PQA1.a	NNP
cord-014597-66vd2mdu	531	12	scale	scale	NN
cord-014597-66vd2mdu	531	13	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	531	14	and	and	CC
cord-014597-66vd2mdu	531	15	high	high	JJ
cord-014597-66vd2mdu	531	16	-	-	HYPH
cord-014597-66vd2mdu	531	17	throughput	throughput	NN
cord-014597-66vd2mdu	531	18	measures	measure	NNS
cord-014597-66vd2mdu	531	19	.	.	.
cord-014597-66vd2mdu	532	1	Its	-PRON-	PRP$
cord-014597-66vd2mdu	532	2	high	high	JJ
cord-014597-66vd2mdu	532	3	repeatability	repeatability	NN
cord-014597-66vd2mdu	532	4	is	be	VBZ
cord-014597-66vd2mdu	532	5	also	also	RB
cord-014597-66vd2mdu	532	6	a	a	DT
cord-014597-66vd2mdu	532	7	key	key	JJ
cord-014597-66vd2mdu	532	8	parameters	parameter	NNS
cord-014597-66vd2mdu	532	9	in	in	IN
cord-014597-66vd2mdu	532	10	the	the	DT
cord-014597-66vd2mdu	532	11	effort	effort	NN
cord-014597-66vd2mdu	532	12	to	to	TO
cord-014597-66vd2mdu	532	13	narrow	narrow	VB
cord-014597-66vd2mdu	532	14	batch	batch	NN
cord-014597-66vd2mdu	532	15	to	to	TO
cord-014597-66vd2mdu	532	16	batch	batch	VB
cord-014597-66vd2mdu	532	17	deviations	deviation	NNS
cord-014597-66vd2mdu	532	18	.	.	.
cord-014597-66vd2mdu	533	1	In	in	IN
cord-014597-66vd2mdu	533	2	addition	addition	NN
cord-014597-66vd2mdu	533	3	we	-PRON-	PRP
cord-014597-66vd2mdu	533	4	demonstrate	demonstrate	VBP
cord-014597-66vd2mdu	533	5	that	that	IN
cord-014597-66vd2mdu	533	6	this	this	DT
cord-014597-66vd2mdu	533	7	technique	technique	NN
cord-014597-66vd2mdu	533	8	is	be	VBZ
cord-014597-66vd2mdu	533	9	potentially	potentially	RB
cord-014597-66vd2mdu	533	10	powerful	powerful	JJ
cord-014597-66vd2mdu	533	11	for	for	IN
cord-014597-66vd2mdu	533	12	in	in	IN
cord-014597-66vd2mdu	533	13	-	-	HYPH
cord-014597-66vd2mdu	533	14	line	line	NN
cord-014597-66vd2mdu	533	15	and	and	CC
cord-014597-66vd2mdu	533	16	continuous	continuous	JJ
cord-014597-66vd2mdu	533	17	monitoring	monitoring	NN
cord-014597-66vd2mdu	533	18	of	of	IN
cord-014597-66vd2mdu	533	19	a	a	DT
cord-014597-66vd2mdu	533	20	lab	lab	NN
cord-014597-66vd2mdu	533	21	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	533	22	.	.	.
cord-014597-66vd2mdu	534	1	We	-PRON-	PRP
cord-014597-66vd2mdu	534	2	envision	envision	VBP
cord-014597-66vd2mdu	534	3	Lensless	Lensless	NNP
cord-014597-66vd2mdu	534	4	Imaging	Imaging	NNP
cord-014597-66vd2mdu	534	5	to	to	TO
cord-014597-66vd2mdu	534	6	become	become	VB
cord-014597-66vd2mdu	534	7	the	the	DT
cord-014597-66vd2mdu	534	8	future	future	JJ
cord-014597-66vd2mdu	534	9	method	method	NN
cord-014597-66vd2mdu	534	10	of	of	IN
cord-014597-66vd2mdu	534	11	choice	choice	NN
cord-014597-66vd2mdu	534	12	for	for	IN
cord-014597-66vd2mdu	534	13	on	on	IN
cord-014597-66vd2mdu	534	14	-	-	HYPH
cord-014597-66vd2mdu	534	15	line	line	NN
cord-014597-66vd2mdu	534	16	and	and	CC
cord-014597-66vd2mdu	534	17	in	in	IN
cord-014597-66vd2mdu	534	18	-	-	HYPH
cord-014597-66vd2mdu	534	19	situ	situ	JJ
cord-014597-66vd2mdu	534	20	monitoring	monitoring	NN
cord-014597-66vd2mdu	534	21	of	of	IN
cord-014597-66vd2mdu	534	22	suspension	suspension	NN
cord-014597-66vd2mdu	534	23	cells	cell	NNS
cord-014597-66vd2mdu	534	24	and	and	CC
cord-014597-66vd2mdu	534	25	a	a	DT
cord-014597-66vd2mdu	534	26	perfect	perfect	JJ
cord-014597-66vd2mdu	534	27	tool	tool	NN
cord-014597-66vd2mdu	534	28	for	for	IN
cord-014597-66vd2mdu	534	29	process	process	NN
cord-014597-66vd2mdu	534	30	control	control	NN
cord-014597-66vd2mdu	534	31	in	in	IN
cord-014597-66vd2mdu	534	32	fed	fed	NNP
cord-014597-66vd2mdu	534	33	-	-	HYPH
cord-014597-66vd2mdu	534	34	batch	batch	NN
cord-014597-66vd2mdu	534	35	or	or	CC
cord-014597-66vd2mdu	534	36	perfusion	perfusion	NN
cord-014597-66vd2mdu	534	37	mode	mode	NN
cord-014597-66vd2mdu	534	38	in	in	IN
cord-014597-66vd2mdu	534	39	single	single	JJ
cord-014597-66vd2mdu	534	40	-	-	HYPH
cord-014597-66vd2mdu	534	41	use	use	NN
cord-014597-66vd2mdu	534	42	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	534	43	or	or	CC
cord-014597-66vd2mdu	534	44	traditional	traditional	JJ
cord-014597-66vd2mdu	534	45	steam	steam	NN
cord-014597-66vd2mdu	534	46	sterilized	sterilize	VBN
cord-014597-66vd2mdu	534	47	vessels	vessel	NNS
cord-014597-66vd2mdu	534	48	.	.	.
cord-014597-66vd2mdu	535	1	It	-PRON-	PRP
cord-014597-66vd2mdu	535	2	can	can	MD
cord-014597-66vd2mdu	535	3	certainly	certainly	RB
cord-014597-66vd2mdu	535	4	become	become	VB
cord-014597-66vd2mdu	535	5	the	the	DT
cord-014597-66vd2mdu	535	6	first	first	JJ
cord-014597-66vd2mdu	535	7	VCD	vcd	NN
cord-014597-66vd2mdu	535	8	measurement	measurement	NN
cord-014597-66vd2mdu	535	9	technique	technique	NN
cord-014597-66vd2mdu	535	10	to	to	TO
cord-014597-66vd2mdu	535	11	work	work	VB
cord-014597-66vd2mdu	535	12	from	from	IN
cord-014597-66vd2mdu	535	13	cell	cell	NN
cord-014597-66vd2mdu	535	14	line	line	NN
cord-014597-66vd2mdu	535	15	engineering	engineering	NN
cord-014597-66vd2mdu	535	16	,	,	,
cord-014597-66vd2mdu	535	17	to	to	TO
cord-014597-66vd2mdu	535	18	process	process	VB
cord-014597-66vd2mdu	535	19	development	development	NN
cord-014597-66vd2mdu	535	20	,	,	,
cord-014597-66vd2mdu	535	21	pilot	pilot	NN
cord-014597-66vd2mdu	535	22	scale	scale	NN
cord-014597-66vd2mdu	535	23	,	,	,
cord-014597-66vd2mdu	535	24	and	and	CC
cord-014597-66vd2mdu	535	25	up	up	IN
cord-014597-66vd2mdu	535	26	to	to	IN
cord-014597-66vd2mdu	535	27	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	535	28	scale	scale	NN
cord-014597-66vd2mdu	535	29	.	.	.
cord-014597-66vd2mdu	536	1	Time	time	NN
cord-014597-66vd2mdu	536	2	-	-	HYPH
cord-014597-66vd2mdu	536	3	dependent	dependent	JJ
cord-014597-66vd2mdu	536	4	product	product	NN
cord-014597-66vd2mdu	536	5	heterogeneity	heterogeneity	NN
cord-014597-66vd2mdu	536	6	in	in	IN
cord-014597-66vd2mdu	536	7	mammalian	mammalian	JJ
cord-014597-66vd2mdu	536	8	cell	cell	NN
cord-014597-66vd2mdu	536	9	fermentation	fermentation	NN
cord-014597-66vd2mdu	536	10	processes	process	NNS
cord-014597-66vd2mdu	537	1	Background	background	VB
cord-014597-66vd2mdu	537	2	A	a	DT
cord-014597-66vd2mdu	537	3	consistent	consistent	JJ
cord-014597-66vd2mdu	537	4	product	product	NN
cord-014597-66vd2mdu	537	5	quality	quality	NN
cord-014597-66vd2mdu	537	6	is	be	VBZ
cord-014597-66vd2mdu	537	7	a	a	DT
cord-014597-66vd2mdu	537	8	major	major	JJ
cord-014597-66vd2mdu	537	9	goal	goal	NN
cord-014597-66vd2mdu	537	10	in	in	IN
cord-014597-66vd2mdu	537	11	the	the	DT
cord-014597-66vd2mdu	537	12	production	production	NN
cord-014597-66vd2mdu	537	13	of	of	IN
cord-014597-66vd2mdu	537	14	biotherapeutics	biotherapeutic	NNS
cord-014597-66vd2mdu	537	15	,	,	,
cord-014597-66vd2mdu	537	16	especially	especially	RB
cord-014597-66vd2mdu	537	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	537	18	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	537	19	.	.	.
cord-014597-66vd2mdu	538	1	Whereas	whereas	IN
cord-014597-66vd2mdu	538	2	it	-PRON-	PRP
cord-014597-66vd2mdu	538	3	is	be	VBZ
cord-014597-66vd2mdu	538	4	unlikely	unlikely	JJ
cord-014597-66vd2mdu	538	5	that	that	IN
cord-014597-66vd2mdu	538	6	the	the	DT
cord-014597-66vd2mdu	538	7	polypeptide	polypeptide	NN
cord-014597-66vd2mdu	538	8	chain	chain	NN
cord-014597-66vd2mdu	538	9	changes	change	NNS
cord-014597-66vd2mdu	538	10	during	during	IN
cord-014597-66vd2mdu	538	11	a	a	DT
cord-014597-66vd2mdu	538	12	production	production	NN
cord-014597-66vd2mdu	538	13	process	process	NN
cord-014597-66vd2mdu	538	14	,	,	,
cord-014597-66vd2mdu	538	15	posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	538	16	modifications	modification	NNS
cord-014597-66vd2mdu	538	17	and	and	CC
cord-014597-66vd2mdu	538	18	protein	protein	NN
cord-014597-66vd2mdu	538	19	folding	folding	NN
cord-014597-66vd2mdu	538	20	are	be	VBP
cord-014597-66vd2mdu	538	21	sensitive	sensitive	JJ
cord-014597-66vd2mdu	538	22	to	to	IN
cord-014597-66vd2mdu	538	23	fluctuations	fluctuation	NNS
cord-014597-66vd2mdu	538	24	in	in	IN
cord-014597-66vd2mdu	538	25	parameters	parameter	NNS
cord-014597-66vd2mdu	538	26	and	and	CC
cord-014597-66vd2mdu	538	27	conditions	condition	NNS
cord-014597-66vd2mdu	538	28	.	.	.
cord-014597-66vd2mdu	539	1	Here	here	RB
cord-014597-66vd2mdu	539	2	we	-PRON-	PRP
cord-014597-66vd2mdu	539	3	focus	focus	VBP
cord-014597-66vd2mdu	539	4	on	on	IN
cord-014597-66vd2mdu	539	5	protein	protein	NN
cord-014597-66vd2mdu	539	6	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	539	7	as	as	IN
cord-014597-66vd2mdu	539	8	one	one	CD
cord-014597-66vd2mdu	539	9	important	important	JJ
cord-014597-66vd2mdu	539	10	indication	indication	NN
cord-014597-66vd2mdu	539	11	for	for	IN
cord-014597-66vd2mdu	539	12	product	product	NN
cord-014597-66vd2mdu	539	13	quality	quality	NN
cord-014597-66vd2mdu	539	14	[	[	-LRB-
cord-014597-66vd2mdu	539	15	1	1	CD
cord-014597-66vd2mdu	539	16	]	]	-RRB-
cord-014597-66vd2mdu	539	17	.	.	.
cord-014597-66vd2mdu	540	1	During	during	IN
cord-014597-66vd2mdu	540	2	a	a	DT
cord-014597-66vd2mdu	540	3	batch	batch	NN
cord-014597-66vd2mdu	540	4	process	process	NN
cord-014597-66vd2mdu	540	5	conditions	condition	NNS
cord-014597-66vd2mdu	540	6	change	change	VBP
cord-014597-66vd2mdu	540	7	continuously	continuously	RB
cord-014597-66vd2mdu	540	8	.	.	.
cord-014597-66vd2mdu	541	1	At	at	IN
cord-014597-66vd2mdu	541	2	the	the	DT
cord-014597-66vd2mdu	541	3	beginning	beginning	NN
cord-014597-66vd2mdu	541	4	,	,	,
cord-014597-66vd2mdu	541	5	the	the	DT
cord-014597-66vd2mdu	541	6	supply	supply	NN
cord-014597-66vd2mdu	541	7	situation	situation	NN
cord-014597-66vd2mdu	541	8	for	for	IN
cord-014597-66vd2mdu	541	9	the	the	DT
cord-014597-66vd2mdu	541	10	cell	cell	NN
cord-014597-66vd2mdu	541	11	is	be	VBZ
cord-014597-66vd2mdu	541	12	excellent	excellent	JJ
cord-014597-66vd2mdu	541	13	,	,	,
cord-014597-66vd2mdu	541	14	but	but	CC
cord-014597-66vd2mdu	541	15	the	the	DT
cord-014597-66vd2mdu	541	16	secreted	secrete	VBN
cord-014597-66vd2mdu	541	17	material	material	NN
cord-014597-66vd2mdu	541	18	stays	stay	VBZ
cord-014597-66vd2mdu	541	19	a	a	DT
cord-014597-66vd2mdu	541	20	long	long	JJ
cord-014597-66vd2mdu	541	21	time	time	NN
cord-014597-66vd2mdu	541	22	in	in	IN
cord-014597-66vd2mdu	541	23	the	the	DT
cord-014597-66vd2mdu	541	24	culture	culture	NN
cord-014597-66vd2mdu	541	25	fluid	fluid	NN
cord-014597-66vd2mdu	541	26	.	.	.
cord-014597-66vd2mdu	542	1	Later	later	RB
cord-014597-66vd2mdu	542	2	during	during	IN
cord-014597-66vd2mdu	542	3	cultivation	cultivation	NN
cord-014597-66vd2mdu	542	4	substrate	substrate	NN
cord-014597-66vd2mdu	542	5	provision	provision	NN
cord-014597-66vd2mdu	542	6	decreases	decrease	NNS
cord-014597-66vd2mdu	542	7	,	,	,
cord-014597-66vd2mdu	542	8	whereas	whereas	IN
cord-014597-66vd2mdu	542	9	the	the	DT
cord-014597-66vd2mdu	542	10	exposition	exposition	NN
cord-014597-66vd2mdu	542	11	time	time	NN
cord-014597-66vd2mdu	542	12	of	of	IN
cord-014597-66vd2mdu	542	13	the	the	DT
cord-014597-66vd2mdu	542	14	protein	protein	NN
cord-014597-66vd2mdu	542	15	to	to	IN
cord-014597-66vd2mdu	542	16	the	the	DT
cord-014597-66vd2mdu	542	17	culture	culture	NN
cord-014597-66vd2mdu	542	18	fluid	fluid	NN
cord-014597-66vd2mdu	542	19	is	be	VBZ
cord-014597-66vd2mdu	542	20	much	much	RB
cord-014597-66vd2mdu	542	21	shorter	short	JJR
cord-014597-66vd2mdu	542	22	.	.	.
cord-014597-66vd2mdu	543	1	Altogether	altogether	RB
cord-014597-66vd2mdu	543	2	this	this	DT
cord-014597-66vd2mdu	543	3	leads	lead	VBZ
cord-014597-66vd2mdu	543	4	to	to	IN
cord-014597-66vd2mdu	543	5	product	product	VB
cord-014597-66vd2mdu	543	6	heterogeneity	heterogeneity	NN
cord-014597-66vd2mdu	543	7	of	of	IN
cord-014597-66vd2mdu	543	8	the	the	DT
cord-014597-66vd2mdu	543	9	secreted	secrete	VBN
cord-014597-66vd2mdu	543	10	protein	protein	NN
cord-014597-66vd2mdu	543	11	during	during	IN
cord-014597-66vd2mdu	543	12	a	a	DT
cord-014597-66vd2mdu	543	13	batch	batch	NN
cord-014597-66vd2mdu	543	14	culture	culture	NN
cord-014597-66vd2mdu	543	15	.	.	.
cord-014597-66vd2mdu	544	1	Four	four	CD
cord-014597-66vd2mdu	544	2	different	different	JJ
cord-014597-66vd2mdu	544	3	cell	cell	NN
cord-014597-66vd2mdu	544	4	lines	line	NNS
cord-014597-66vd2mdu	544	5	,	,	,
cord-014597-66vd2mdu	544	6	two	two	CD
cord-014597-66vd2mdu	544	7	of	of	IN
cord-014597-66vd2mdu	544	8	human	human	JJ
cord-014597-66vd2mdu	544	9	origin	origin	NN
cord-014597-66vd2mdu	544	10	and	and	CC
cord-014597-66vd2mdu	544	11	two	two	CD
cord-014597-66vd2mdu	544	12	CHO	CHO	NNP
cord-014597-66vd2mdu	544	13	clones	clone	NNS
cord-014597-66vd2mdu	544	14	,	,	,
cord-014597-66vd2mdu	544	15	producing	produce	VBG
cord-014597-66vd2mdu	544	16	four	four	CD
cord-014597-66vd2mdu	544	17	different	different	JJ
cord-014597-66vd2mdu	544	18	recombinant	recombinant	JJ
cord-014597-66vd2mdu	544	19	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	544	20	were	be	VBD
cord-014597-66vd2mdu	544	21	investigated	investigate	VBN
cord-014597-66vd2mdu	544	22	in	in	IN
cord-014597-66vd2mdu	544	23	this	this	DT
cord-014597-66vd2mdu	544	24	study	study	NN
cord-014597-66vd2mdu	544	25	.	.	.
cord-014597-66vd2mdu	545	1	Together	together	RB
cord-014597-66vd2mdu	545	2	with	with	IN
cord-014597-66vd2mdu	545	3	their	-PRON-	PRP$
cord-014597-66vd2mdu	545	4	respective	respective	JJ
cord-014597-66vd2mdu	545	5	parental	parental	JJ
cord-014597-66vd2mdu	545	6	cell	cell	NN
cord-014597-66vd2mdu	545	7	line	line	NN
cord-014597-66vd2mdu	545	8	the	the	DT
cord-014597-66vd2mdu	545	9	clones	clone	NNS
cord-014597-66vd2mdu	545	10	were	be	VBD
cord-014597-66vd2mdu	545	11	cultured	culture	VBN
cord-014597-66vd2mdu	545	12	in	in	IN
cord-014597-66vd2mdu	545	13	three	three	CD
cord-014597-66vd2mdu	545	14	replicates	replicate	NNS
cord-014597-66vd2mdu	545	15	in	in	IN
cord-014597-66vd2mdu	545	16	shakers	shaker	NNS
cord-014597-66vd2mdu	545	17	.	.	.
cord-014597-66vd2mdu	546	1	Supernatant	supernatant	JJ
cord-014597-66vd2mdu	546	2	from	from	IN
cord-014597-66vd2mdu	546	3	the	the	DT
cord-014597-66vd2mdu	546	4	cultures	culture	NNS
cord-014597-66vd2mdu	546	5	were	be	VBD
cord-014597-66vd2mdu	546	6	harvested	harvest	VBN
cord-014597-66vd2mdu	546	7	at	at	IN
cord-014597-66vd2mdu	546	8	four	four	CD
cord-014597-66vd2mdu	546	9	time	time	NN
cord-014597-66vd2mdu	546	10	points	point	NNS
cord-014597-66vd2mdu	546	11	.	.	.
cord-014597-66vd2mdu	547	1	The	the	DT
cord-014597-66vd2mdu	547	2	removed	remove	VBN
cord-014597-66vd2mdu	547	3	culture	culture	NN
cord-014597-66vd2mdu	547	4	volume	volume	NN
cord-014597-66vd2mdu	547	5	was	be	VBD
cord-014597-66vd2mdu	547	6	replaced	replace	VBN
cord-014597-66vd2mdu	547	7	by	by	IN
cord-014597-66vd2mdu	547	8	culture	culture	NN
cord-014597-66vd2mdu	547	9	supernatant	supernatant	JJ
cord-014597-66vd2mdu	547	10	of	of	IN
cord-014597-66vd2mdu	547	11	the	the	DT
cord-014597-66vd2mdu	547	12	identically	identically	RB
cord-014597-66vd2mdu	547	13	cultured	cultured	JJ
cord-014597-66vd2mdu	547	14	corresponding	correspond	VBG
cord-014597-66vd2mdu	547	15	parental	parental	JJ
cord-014597-66vd2mdu	547	16	cell	cell	NN
cord-014597-66vd2mdu	547	17	line	line	NN
cord-014597-66vd2mdu	547	18	.	.	.
cord-014597-66vd2mdu	548	1	The	the	DT
cord-014597-66vd2mdu	548	2	product	product	NN
cord-014597-66vd2mdu	548	3	was	be	VBD
cord-014597-66vd2mdu	548	4	isolated	isolate	VBN
cord-014597-66vd2mdu	548	5	from	from	IN
cord-014597-66vd2mdu	548	6	the	the	DT
cord-014597-66vd2mdu	548	7	supernatant	supernatant	NN
cord-014597-66vd2mdu	548	8	and	and	CC
cord-014597-66vd2mdu	548	9	the	the	DT
cord-014597-66vd2mdu	548	10	glycans	glycan	NNS
cord-014597-66vd2mdu	548	11	were	be	VBD
cord-014597-66vd2mdu	548	12	released	release	VBN
cord-014597-66vd2mdu	548	13	.	.	.
cord-014597-66vd2mdu	549	1	One	one	CD
cord-014597-66vd2mdu	549	2	part	part	NN
cord-014597-66vd2mdu	549	3	of	of	IN
cord-014597-66vd2mdu	549	4	the	the	DT
cord-014597-66vd2mdu	549	5	released	release	VBN
cord-014597-66vd2mdu	549	6	glycans	glycan	NNS
cord-014597-66vd2mdu	549	7	was	be	VBD
cord-014597-66vd2mdu	549	8	labeled	label	VBN
cord-014597-66vd2mdu	549	9	with	with	IN
cord-014597-66vd2mdu	549	10	2-AB	2-ab	CD
cord-014597-66vd2mdu	549	11	and	and	CC
cord-014597-66vd2mdu	549	12	separated	separate	VBN
cord-014597-66vd2mdu	549	13	by	by	IN
cord-014597-66vd2mdu	549	14	HILIC	HILIC	NNP
cord-014597-66vd2mdu	549	15	-	-	HYPH
cord-014597-66vd2mdu	549	16	FLD	FLD	NNP
cord-014597-66vd2mdu	549	17	.	.	.
cord-014597-66vd2mdu	550	1	The	the	DT
cord-014597-66vd2mdu	550	2	other	other	JJ
cord-014597-66vd2mdu	550	3	part	part	NN
cord-014597-66vd2mdu	550	4	of	of	IN
cord-014597-66vd2mdu	550	5	the	the	DT
cord-014597-66vd2mdu	550	6	glycans	glycan	NNS
cord-014597-66vd2mdu	550	7	was	be	VBD
cord-014597-66vd2mdu	550	8	permethylated	permethylate	VBN
cord-014597-66vd2mdu	550	9	and	and	CC
cord-014597-66vd2mdu	550	10	analyzed	analyze	VBN
cord-014597-66vd2mdu	550	11	by	by	IN
cord-014597-66vd2mdu	550	12	MALDI	MALDI	NNP
cord-014597-66vd2mdu	550	13	-	-	HYPH
cord-014597-66vd2mdu	550	14	ToF	ToF	NNP
cord-014597-66vd2mdu	550	15	mass	mass	NN
cord-014597-66vd2mdu	550	16	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	550	17	(	(	-LRB-
cord-014597-66vd2mdu	550	18	Fig	Fig	NNP
cord-014597-66vd2mdu	550	19	.	.	.
cord-014597-66vd2mdu	550	20	1a	1a	CD
cord-014597-66vd2mdu	550	21	)	)	-RRB-
cord-014597-66vd2mdu	550	22	.	.	.
cord-014597-66vd2mdu	551	1	The	the	DT
cord-014597-66vd2mdu	551	2	investigated	investigate	VBN
cord-014597-66vd2mdu	551	3	proteins	protein	NNS
cord-014597-66vd2mdu	551	4	were	be	VBD
cord-014597-66vd2mdu	551	5	Antibody	Antibody	NNP
cord-014597-66vd2mdu	551	6	,	,	,
cord-014597-66vd2mdu	551	7	Antithrombin	Antithrombin	NNP
cord-014597-66vd2mdu	551	8	III	III	NNP
cord-014597-66vd2mdu	551	9	from	from	IN
cord-014597-66vd2mdu	551	10	CHO	CHO	NNP
cord-014597-66vd2mdu	551	11	clones	clone	NNS
cord-014597-66vd2mdu	551	12	and	and	CC
cord-014597-66vd2mdu	551	13	α1-Antitrypsin	α1-Antitrypsin	NNP
cord-014597-66vd2mdu	551	14	,	,	,
cord-014597-66vd2mdu	551	15	C1-Inhibitor	C1-Inhibitor	NNP
cord-014597-66vd2mdu	551	16	from	from	IN
cord-014597-66vd2mdu	551	17	human	human	JJ
cord-014597-66vd2mdu	551	18	clones	clone	NNS
cord-014597-66vd2mdu	551	19	.	.	.
cord-014597-66vd2mdu	552	1	The	the	DT
cord-014597-66vd2mdu	552	2	antennarity	antennarity	NN
cord-014597-66vd2mdu	552	3	of	of	IN
cord-014597-66vd2mdu	552	4	the	the	DT
cord-014597-66vd2mdu	552	5	glycans	glycan	NNS
cord-014597-66vd2mdu	552	6	is	be	VBZ
cord-014597-66vd2mdu	552	7	quite	quite	RB
cord-014597-66vd2mdu	552	8	stable	stable	JJ
cord-014597-66vd2mdu	552	9	in	in	IN
cord-014597-66vd2mdu	552	10	all	all	DT
cord-014597-66vd2mdu	552	11	production	production	NN
cord-014597-66vd2mdu	552	12	phases	phase	NNS
cord-014597-66vd2mdu	552	13	.	.	.
cord-014597-66vd2mdu	553	1	The	the	DT
cord-014597-66vd2mdu	553	2	degree	degree	NN
cord-014597-66vd2mdu	553	3	of	of	IN
cord-014597-66vd2mdu	553	4	core	core	NN
cord-014597-66vd2mdu	553	5	fucosylation	fucosylation	NN
cord-014597-66vd2mdu	553	6	is	be	VBZ
cord-014597-66vd2mdu	553	7	very	very	RB
cord-014597-66vd2mdu	553	8	high	high	JJ
cord-014597-66vd2mdu	553	9	in	in	IN
cord-014597-66vd2mdu	553	10	all	all	DT
cord-014597-66vd2mdu	553	11	products	product	NNS
cord-014597-66vd2mdu	553	12	.	.	.
cord-014597-66vd2mdu	554	1	A	a	DT
cord-014597-66vd2mdu	554	2	low	low	JJ
cord-014597-66vd2mdu	554	3	fucosylation	fucosylation	NN
cord-014597-66vd2mdu	554	4	degree	degree	NN
cord-014597-66vd2mdu	554	5	of	of	IN
cord-014597-66vd2mdu	554	6	antibodies	antibody	NNS
cord-014597-66vd2mdu	554	7	may	may	MD
cord-014597-66vd2mdu	554	8	be	be	VB
cord-014597-66vd2mdu	554	9	favorable	favorable	JJ
cord-014597-66vd2mdu	554	10	for	for	IN
cord-014597-66vd2mdu	554	11	a	a	DT
cord-014597-66vd2mdu	554	12	higher	high	JJR
cord-014597-66vd2mdu	554	13	ADCC	ADCC	NNP
cord-014597-66vd2mdu	554	14	performance	performance	NN
cord-014597-66vd2mdu	554	15	[	[	-LRB-
cord-014597-66vd2mdu	554	16	2	2	CD
cord-014597-66vd2mdu	554	17	]	]	-RRB-
cord-014597-66vd2mdu	554	18	.	.	.
cord-014597-66vd2mdu	555	1	Some	some	DT
cord-014597-66vd2mdu	555	2	of	of	IN
cord-014597-66vd2mdu	555	3	the	the	DT
cord-014597-66vd2mdu	555	4	products	product	NNS
cord-014597-66vd2mdu	555	5	showed	show	VBD
cord-014597-66vd2mdu	555	6	an	an	DT
cord-014597-66vd2mdu	555	7	antennary	antennary	JJ
cord-014597-66vd2mdu	555	8	fucosylation	fucosylation	NN
cord-014597-66vd2mdu	555	9	,	,	,
cord-014597-66vd2mdu	555	10	which	which	WDT
cord-014597-66vd2mdu	555	11	seemed	seem	VBD
cord-014597-66vd2mdu	555	12	to	to	TO
cord-014597-66vd2mdu	555	13	change	change	VB
cord-014597-66vd2mdu	555	14	not	not	RB
cord-014597-66vd2mdu	555	15	very	very	RB
cord-014597-66vd2mdu	555	16	much	much	RB
cord-014597-66vd2mdu	555	17	in	in	IN
cord-014597-66vd2mdu	555	18	different	different	JJ
cord-014597-66vd2mdu	555	19	cultivation	cultivation	NN
cord-014597-66vd2mdu	555	20	phases	phase	NNS
cord-014597-66vd2mdu	555	21	.	.	.
cord-014597-66vd2mdu	556	1	Nevertheless	nevertheless	RB
cord-014597-66vd2mdu	556	2	,	,	,
cord-014597-66vd2mdu	556	3	this	this	DT
cord-014597-66vd2mdu	556	4	might	may	MD
cord-014597-66vd2mdu	556	5	be	be	VB
cord-014597-66vd2mdu	556	6	an	an	DT
cord-014597-66vd2mdu	556	7	issue	issue	NN
cord-014597-66vd2mdu	556	8	due	due	IN
cord-014597-66vd2mdu	556	9	to	to	IN
cord-014597-66vd2mdu	556	10	an	an	DT
cord-014597-66vd2mdu	556	11	antigenic	antigenic	JJ
cord-014597-66vd2mdu	556	12	impact	impact	NN
cord-014597-66vd2mdu	556	13	in	in	IN
cord-014597-66vd2mdu	556	14	the	the	DT
cord-014597-66vd2mdu	556	15	patient	patient	NN
cord-014597-66vd2mdu	556	16	.	.	.
cord-014597-66vd2mdu	557	1	The	the	DT
cord-014597-66vd2mdu	557	2	antennary	antennary	JJ
cord-014597-66vd2mdu	557	3	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	557	4	changes	change	VBZ
cord-014597-66vd2mdu	557	5	noticeable	noticeable	JJ
cord-014597-66vd2mdu	557	6	for	for	IN
cord-014597-66vd2mdu	557	7	the	the	DT
cord-014597-66vd2mdu	557	8	antibody	antibody	NN
cord-014597-66vd2mdu	557	9	and	and	CC
cord-014597-66vd2mdu	557	10	α1-Antitrypsin	α1-Antitrypsin	NNP
cord-014597-66vd2mdu	557	11	.	.	.
cord-014597-66vd2mdu	558	1	In	in	IN
cord-014597-66vd2mdu	558	2	both	both	DT
cord-014597-66vd2mdu	558	3	cases	case	NNS
cord-014597-66vd2mdu	558	4	the	the	DT
cord-014597-66vd2mdu	558	5	degree	degree	NN
cord-014597-66vd2mdu	558	6	is	be	VBZ
cord-014597-66vd2mdu	558	7	highest	high	JJS
cord-014597-66vd2mdu	558	8	in	in	IN
cord-014597-66vd2mdu	558	9	the	the	DT
cord-014597-66vd2mdu	558	10	first	first	JJ
cord-014597-66vd2mdu	558	11	phase	phase	NN
cord-014597-66vd2mdu	558	12	.	.	.
cord-014597-66vd2mdu	559	1	An	an	DT
cord-014597-66vd2mdu	559	2	incomplete	incomplete	JJ
cord-014597-66vd2mdu	559	3	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	559	4	leads	lead	VBZ
cord-014597-66vd2mdu	559	5	to	to	IN
cord-014597-66vd2mdu	559	6	truncated	truncated	JJ
cord-014597-66vd2mdu	559	7	glycans	glycan	NNS
cord-014597-66vd2mdu	559	8	.	.	.
cord-014597-66vd2mdu	560	1	This	this	DT
cord-014597-66vd2mdu	560	2	leads	lead	VBZ
cord-014597-66vd2mdu	560	3	inevitable	inevitable	JJ
cord-014597-66vd2mdu	560	4	to	to	IN
cord-014597-66vd2mdu	560	5	undersialylated	undersialylated	JJ
cord-014597-66vd2mdu	560	6	antennas	antenna	NNS
cord-014597-66vd2mdu	560	7	to	to	TO
cord-014597-66vd2mdu	560	8	be	be	VB
cord-014597-66vd2mdu	560	9	seen	see	VBN
cord-014597-66vd2mdu	560	10	for	for	IN
cord-014597-66vd2mdu	560	11	α1-Antitrypsin	α1-Antitrypsin	NNP
cord-014597-66vd2mdu	560	12	.	.	.
cord-014597-66vd2mdu	561	1	The	the	DT
cord-014597-66vd2mdu	561	2	sialylation	sialylation	NN
cord-014597-66vd2mdu	561	3	is	be	VBZ
cord-014597-66vd2mdu	561	4	the	the	DT
cord-014597-66vd2mdu	561	5	highest	high	JJS
cord-014597-66vd2mdu	561	6	in	in	IN
cord-014597-66vd2mdu	561	7	the	the	DT
cord-014597-66vd2mdu	561	8	early	early	JJ
cord-014597-66vd2mdu	561	9	phases	phase	NNS
cord-014597-66vd2mdu	561	10	and	and	CC
cord-014597-66vd2mdu	561	11	decreases	decrease	NNS
cord-014597-66vd2mdu	561	12	during	during	IN
cord-014597-66vd2mdu	561	13	cultivation	cultivation	NN
cord-014597-66vd2mdu	561	14	time	time	NN
cord-014597-66vd2mdu	561	15	.	.	.
cord-014597-66vd2mdu	562	1	Sialylation	sialylation	NN
cord-014597-66vd2mdu	562	2	of	of	IN
cord-014597-66vd2mdu	562	3	a	a	DT
cord-014597-66vd2mdu	562	4	therapeutical	therapeutical	JJ
cord-014597-66vd2mdu	562	5	protein	protein	NN
cord-014597-66vd2mdu	562	6	is	be	VBZ
cord-014597-66vd2mdu	562	7	important	important	JJ
cord-014597-66vd2mdu	562	8	for	for	IN
cord-014597-66vd2mdu	562	9	the	the	DT
cord-014597-66vd2mdu	562	10	half	half	JJ
cord-014597-66vd2mdu	562	11	-	-	HYPH
cord-014597-66vd2mdu	562	12	life	life	NN
cord-014597-66vd2mdu	562	13	in	in	IN
cord-014597-66vd2mdu	562	14	the	the	DT
cord-014597-66vd2mdu	562	15	patient	patient	NN
cord-014597-66vd2mdu	562	16	.	.	.
cord-014597-66vd2mdu	563	1	Therefore	therefore	RB
cord-014597-66vd2mdu	563	2	highly	highly	RB
cord-014597-66vd2mdu	563	3	sialylated	sialylate	VBN
cord-014597-66vd2mdu	563	4	products	product	NNS
cord-014597-66vd2mdu	563	5	are	be	VBP
cord-014597-66vd2mdu	563	6	desired	desire	VBN
cord-014597-66vd2mdu	563	7	[	[	-LRB-
cord-014597-66vd2mdu	563	8	3	3	CD
cord-014597-66vd2mdu	563	9	]	]	-RRB-
cord-014597-66vd2mdu	563	10	.	.	.
cord-014597-66vd2mdu	564	1	In	in	IN
cord-014597-66vd2mdu	564	2	further	further	JJ
cord-014597-66vd2mdu	564	3	studies	study	NNS
cord-014597-66vd2mdu	564	4	the	the	DT
cord-014597-66vd2mdu	564	5	consistency	consistency	NN
cord-014597-66vd2mdu	564	6	of	of	IN
cord-014597-66vd2mdu	564	7	the	the	DT
cord-014597-66vd2mdu	564	8	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	564	9	and	and	CC
cord-014597-66vd2mdu	564	10	the	the	DT
cord-014597-66vd2mdu	564	11	sialylation	sialylation	NN
cord-014597-66vd2mdu	564	12	will	will	MD
cord-014597-66vd2mdu	564	13	investigated	investigate	VBN
cord-014597-66vd2mdu	564	14	for	for	IN
cord-014597-66vd2mdu	564	15	fed	fed	NNP
cord-014597-66vd2mdu	564	16	batch	batch	NN
cord-014597-66vd2mdu	564	17	and	and	CC
cord-014597-66vd2mdu	564	18	long	long	JJ
cord-014597-66vd2mdu	564	19	term	term	NN
cord-014597-66vd2mdu	564	20	continuous	continuous	JJ
cord-014597-66vd2mdu	564	21	cultures	culture	NNS
cord-014597-66vd2mdu	564	22	in	in	IN
cord-014597-66vd2mdu	564	23	comparison	comparison	NN
cord-014597-66vd2mdu	564	24	to	to	TO
cord-014597-66vd2mdu	564	25	batch	batch	VB
cord-014597-66vd2mdu	564	26	cultures	culture	NNS
cord-014597-66vd2mdu	564	27	.	.	.
cord-014597-66vd2mdu	565	1	Due	due	IN
cord-014597-66vd2mdu	565	2	to	to	IN
cord-014597-66vd2mdu	565	3	the	the	DT
cord-014597-66vd2mdu	565	4	feed	feed	NN
cord-014597-66vd2mdu	565	5	solution	solution	NN
cord-014597-66vd2mdu	565	6	or	or	CC
cord-014597-66vd2mdu	565	7	the	the	DT
cord-014597-66vd2mdu	565	8	fresh	fresh	JJ
cord-014597-66vd2mdu	565	9	media	medium	NNS
cord-014597-66vd2mdu	565	10	being	be	VBG
cord-014597-66vd2mdu	565	11	present	present	JJ
cord-014597-66vd2mdu	565	12	during	during	IN
cord-014597-66vd2mdu	565	13	such	such	JJ
cord-014597-66vd2mdu	565	14	processes	process	NNS
cord-014597-66vd2mdu	565	15	,	,	,
cord-014597-66vd2mdu	565	16	the	the	DT
cord-014597-66vd2mdu	565	17	supply	supply	NN
cord-014597-66vd2mdu	565	18	situation	situation	NN
cord-014597-66vd2mdu	565	19	should	should	MD
cord-014597-66vd2mdu	565	20	be	be	VB
cord-014597-66vd2mdu	565	21	excellent	excellent	JJ
cord-014597-66vd2mdu	565	22	for	for	IN
cord-014597-66vd2mdu	565	23	the	the	DT
cord-014597-66vd2mdu	565	24	whole	whole	JJ
cord-014597-66vd2mdu	565	25	cultivation	cultivation	NN
cord-014597-66vd2mdu	565	26	time	time	NN
cord-014597-66vd2mdu	565	27	.	.	.
cord-014597-66vd2mdu	566	1	The	the	DT
cord-014597-66vd2mdu	566	2	differences	difference	NNS
cord-014597-66vd2mdu	566	3	between	between	IN
cord-014597-66vd2mdu	566	4	the	the	DT
cord-014597-66vd2mdu	566	5	MALDI	MALDI	NNP
cord-014597-66vd2mdu	566	6	-	-	HYPH
cord-014597-66vd2mdu	566	7	ToF	ToF	NNP
cord-014597-66vd2mdu	566	8	and	and	CC
cord-014597-66vd2mdu	566	9	HILIC	HILIC	NNP
cord-014597-66vd2mdu	566	10	-	-	HYPH
cord-014597-66vd2mdu	566	11	FLD	FLD	NNP
cord-014597-66vd2mdu	566	12	data	datum	NNS
cord-014597-66vd2mdu	566	13	originate	originate	VBP
cord-014597-66vd2mdu	566	14	from	from	IN
cord-014597-66vd2mdu	566	15	complex	complex	JJ
cord-014597-66vd2mdu	566	16	and	and	CC
cord-014597-66vd2mdu	566	17	unresolved	unresolved	JJ
cord-014597-66vd2mdu	566	18	chromatograms	chromatogram	NNS
cord-014597-66vd2mdu	566	19	(	(	-LRB-
cord-014597-66vd2mdu	566	20	Fig	Fig	NNP
cord-014597-66vd2mdu	566	21	.	.	.
cord-014597-66vd2mdu	567	1	1b	1b	NNP
cord-014597-66vd2mdu	567	2	,	,	,
cord-014597-66vd2mdu	567	3	chromatograms	chromatograms	NNP
cord-014597-66vd2mdu	567	4	not	not	RB
cord-014597-66vd2mdu	567	5	shown	show	VBN
cord-014597-66vd2mdu	567	6	)	)	-RRB-
cord-014597-66vd2mdu	567	7	.	.	.
cord-014597-66vd2mdu	568	1	For	for	IN
cord-014597-66vd2mdu	568	2	that	that	DT
cord-014597-66vd2mdu	568	3	reason	reason	NN
cord-014597-66vd2mdu	568	4	coupling	coupling	NN
cord-014597-66vd2mdu	568	5	of	of	IN
cord-014597-66vd2mdu	568	6	HILIC	HILIC	NNP
cord-014597-66vd2mdu	568	7	-	-	HYPH
cord-014597-66vd2mdu	568	8	FLD	FLD	NNP
cord-014597-66vd2mdu	568	9	and	and	CC
cord-014597-66vd2mdu	568	10	MS	MS	NNP
cord-014597-66vd2mdu	568	11	is	be	VBZ
cord-014597-66vd2mdu	568	12	very	very	RB
cord-014597-66vd2mdu	568	13	much	much	RB
cord-014597-66vd2mdu	568	14	recommended	recommend	VBN
cord-014597-66vd2mdu	568	15	.	.	.
cord-014597-66vd2mdu	569	1	Background	Background	NNP
cord-014597-66vd2mdu	569	2	Novel	Novel	NNP
cord-014597-66vd2mdu	569	3	biologics	biologic	NNS
cord-014597-66vd2mdu	569	4	are	be	VBP
cord-014597-66vd2mdu	569	5	often	often	RB
cord-014597-66vd2mdu	569	6	selected	select	VBN
cord-014597-66vd2mdu	569	7	from	from	IN
cord-014597-66vd2mdu	569	8	a	a	DT
cord-014597-66vd2mdu	569	9	large	large	JJ
cord-014597-66vd2mdu	569	10	library	library	NN
cord-014597-66vd2mdu	569	11	of	of	IN
cord-014597-66vd2mdu	569	12	lead	lead	JJ
cord-014597-66vd2mdu	569	13	candidates	candidate	NNS
cord-014597-66vd2mdu	569	14	in	in	IN
cord-014597-66vd2mdu	569	15	the	the	DT
cord-014597-66vd2mdu	569	16	initial	initial	JJ
cord-014597-66vd2mdu	569	17	stage	stage	NN
cord-014597-66vd2mdu	569	18	of	of	IN
cord-014597-66vd2mdu	569	19	preclinical	preclinical	JJ
cord-014597-66vd2mdu	569	20	and	and	CC
cord-014597-66vd2mdu	569	21	clinical	clinical	JJ
cord-014597-66vd2mdu	569	22	developments	development	NNS
cord-014597-66vd2mdu	569	23	.	.	.
cord-014597-66vd2mdu	570	1	For	for	IN
cord-014597-66vd2mdu	570	2	this	this	DT
cord-014597-66vd2mdu	570	3	selection	selection	NN
cord-014597-66vd2mdu	570	4	,	,	,
cord-014597-66vd2mdu	570	5	there	there	EX
cord-014597-66vd2mdu	570	6	is	be	VBZ
cord-014597-66vd2mdu	570	7	a	a	DT
cord-014597-66vd2mdu	570	8	demand	demand	NN
cord-014597-66vd2mdu	570	9	for	for	IN
cord-014597-66vd2mdu	570	10	high	high	JJ
cord-014597-66vd2mdu	570	11	-	-	HYPH
cord-014597-66vd2mdu	570	12	throughput	throughput	NN
cord-014597-66vd2mdu	570	13	production	production	NN
cord-014597-66vd2mdu	570	14	of	of	IN
cord-014597-66vd2mdu	570	15	recombinant	recombinant	JJ
cord-014597-66vd2mdu	570	16	proteins	protein	NNS
cord-014597-66vd2mdu	570	17	of	of	IN
cord-014597-66vd2mdu	570	18	high	high	JJ
cord-014597-66vd2mdu	570	19	quality	quality	NN
cord-014597-66vd2mdu	570	20	and	and	CC
cord-014597-66vd2mdu	570	21	in	in	IN
cord-014597-66vd2mdu	570	22	sufficient	sufficient	JJ
cord-014597-66vd2mdu	570	23	quantity	quantity	NN
cord-014597-66vd2mdu	570	24	.	.	.
cord-014597-66vd2mdu	571	1	Transient	transient	JJ
cord-014597-66vd2mdu	571	2	gene	gene	NN
cord-014597-66vd2mdu	571	3	expression	expression	NN
cord-014597-66vd2mdu	571	4	offers	offer	VBZ
cord-014597-66vd2mdu	571	5	a	a	DT
cord-014597-66vd2mdu	571	6	rapid	rapid	JJ
cord-014597-66vd2mdu	571	7	approach	approach	NN
cord-014597-66vd2mdu	571	8	to	to	IN
cord-014597-66vd2mdu	571	9	the	the	DT
cord-014597-66vd2mdu	571	10	production	production	NN
cord-014597-66vd2mdu	571	11	of	of	IN
cord-014597-66vd2mdu	571	12	numerous	numerous	JJ
cord-014597-66vd2mdu	571	13	recombinant	recombinant	JJ
cord-014597-66vd2mdu	571	14	proteins	protein	NNS
cord-014597-66vd2mdu	571	15	for	for	IN
cord-014597-66vd2mdu	571	16	the	the	DT
cord-014597-66vd2mdu	571	17	initial	initial	JJ
cord-014597-66vd2mdu	571	18	-	-	HYPH
cord-014597-66vd2mdu	571	19	stage	stage	NN
cord-014597-66vd2mdu	571	20	developments	development	NNS
cord-014597-66vd2mdu	571	21	of	of	IN
cord-014597-66vd2mdu	571	22	biologics	biologic	NNS
cord-014597-66vd2mdu	571	23	.	.	.
cord-014597-66vd2mdu	572	1	Mammalian	mammalian	JJ
cord-014597-66vd2mdu	572	2	cells	cell	NNS
cord-014597-66vd2mdu	572	3	are	be	VBP
cord-014597-66vd2mdu	572	4	major	major	JJ
cord-014597-66vd2mdu	572	5	host	host	NN
cord-014597-66vd2mdu	572	6	cells	cell	NNS
cord-014597-66vd2mdu	572	7	for	for	IN
cord-014597-66vd2mdu	572	8	transient	transient	JJ
cord-014597-66vd2mdu	572	9	gene	gene	NN
cord-014597-66vd2mdu	572	10	expression	expression	NN
cord-014597-66vd2mdu	572	11	,	,	,
cord-014597-66vd2mdu	572	12	but	but	CC
cord-014597-66vd2mdu	572	13	they	-PRON-	PRP
cord-014597-66vd2mdu	572	14	have	have	VBP
cord-014597-66vd2mdu	572	15	the	the	DT
cord-014597-66vd2mdu	572	16	disadvantages	disadvantage	NNS
cord-014597-66vd2mdu	572	17	of	of	IN
cord-014597-66vd2mdu	572	18	complicated	complicated	JJ
cord-014597-66vd2mdu	572	19	operations	operation	NNS
cord-014597-66vd2mdu	572	20	and	and	CC
cord-014597-66vd2mdu	572	21	high	high	JJ
cord-014597-66vd2mdu	572	22	cost	cost	NN
cord-014597-66vd2mdu	572	23	of	of	IN
cord-014597-66vd2mdu	572	24	culture	culture	NN
cord-014597-66vd2mdu	572	25	.	.	.
cord-014597-66vd2mdu	573	1	Insect	insect	NN
cord-014597-66vd2mdu	573	2	cells	cell	NNS
cord-014597-66vd2mdu	573	3	are	be	VBP
cord-014597-66vd2mdu	573	4	easy	easy	JJ
cord-014597-66vd2mdu	573	5	to	to	TO
cord-014597-66vd2mdu	573	6	handle	handle	VB
cord-014597-66vd2mdu	573	7	and	and	CC
cord-014597-66vd2mdu	573	8	can	can	MD
cord-014597-66vd2mdu	573	9	be	be	VB
cord-014597-66vd2mdu	573	10	grown	grow	VBN
cord-014597-66vd2mdu	573	11	to	to	IN
cord-014597-66vd2mdu	573	12	a	a	DT
cord-014597-66vd2mdu	573	13	high	high	JJ
cord-014597-66vd2mdu	573	14	cell	cell	NN
cord-014597-66vd2mdu	573	15	density	density	NN
cord-014597-66vd2mdu	573	16	in	in	IN
cord-014597-66vd2mdu	573	17	suspension	suspension	NN
cord-014597-66vd2mdu	573	18	with	with	IN
cord-014597-66vd2mdu	573	19	a	a	DT
cord-014597-66vd2mdu	573	20	serum	serum	NN
cord-014597-66vd2mdu	573	21	-	-	HYPH
cord-014597-66vd2mdu	573	22	free	free	JJ
cord-014597-66vd2mdu	573	23	medium	medium	NN
cord-014597-66vd2mdu	573	24	.	.	.
cord-014597-66vd2mdu	574	1	Insect	insect	NN
cord-014597-66vd2mdu	574	2	cells	cell	NNS
cord-014597-66vd2mdu	574	3	can	can	MD
cord-014597-66vd2mdu	574	4	also	also	RB
cord-014597-66vd2mdu	574	5	produce	produce	VB
cord-014597-66vd2mdu	574	6	large	large	JJ
cord-014597-66vd2mdu	574	7	amount	amount	NN
cord-014597-66vd2mdu	574	8	of	of	IN
cord-014597-66vd2mdu	574	9	recombinant	recombinant	JJ
cord-014597-66vd2mdu	574	10	proteins	protein	NNS
cord-014597-66vd2mdu	574	11	through	through	IN
cord-014597-66vd2mdu	574	12	post	post	JJ
cord-014597-66vd2mdu	574	13	-	-	JJ
cord-014597-66vd2mdu	574	14	translational	translational	JJ
cord-014597-66vd2mdu	574	15	processing	processing	NN
cord-014597-66vd2mdu	574	16	and	and	CC
cord-014597-66vd2mdu	574	17	modifications	modification	NNS
cord-014597-66vd2mdu	574	18	of	of	IN
cord-014597-66vd2mdu	574	19	higher	high	JJR
cord-014597-66vd2mdu	574	20	eukaryotes	eukaryote	NNS
cord-014597-66vd2mdu	574	21	.	.	.
cord-014597-66vd2mdu	575	1	Hence	hence	RB
cord-014597-66vd2mdu	575	2	,	,	,
cord-014597-66vd2mdu	575	3	insect	insect	NN
cord-014597-66vd2mdu	575	4	cells	cell	NNS
cord-014597-66vd2mdu	575	5	have	have	VBP
cord-014597-66vd2mdu	575	6	been	be	VBN
cord-014597-66vd2mdu	575	7	recognized	recognize	VBN
cord-014597-66vd2mdu	575	8	as	as	IN
cord-014597-66vd2mdu	575	9	an	an	DT
cord-014597-66vd2mdu	575	10	excellent	excellent	JJ
cord-014597-66vd2mdu	575	11	platform	platform	NN
cord-014597-66vd2mdu	575	12	for	for	IN
cord-014597-66vd2mdu	575	13	the	the	DT
cord-014597-66vd2mdu	575	14	production	production	NN
cord-014597-66vd2mdu	575	15	of	of	IN
cord-014597-66vd2mdu	575	16	functional	functional	JJ
cord-014597-66vd2mdu	575	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	575	18	proteins	protein	NNS
cord-014597-66vd2mdu	575	19	[	[	-LRB-
cord-014597-66vd2mdu	575	20	1	1	CD
cord-014597-66vd2mdu	575	21	,	,	,
cord-014597-66vd2mdu	575	22	2	2	CD
cord-014597-66vd2mdu	575	23	]	]	-RRB-
cord-014597-66vd2mdu	575	24	.	.	.
cord-014597-66vd2mdu	576	1	In	in	IN
cord-014597-66vd2mdu	576	2	the	the	DT
cord-014597-66vd2mdu	576	3	present	present	JJ
cord-014597-66vd2mdu	576	4	study	study	NN
cord-014597-66vd2mdu	576	5	,	,	,
cord-014597-66vd2mdu	576	6	the	the	DT
cord-014597-66vd2mdu	576	7	production	production	NN
cord-014597-66vd2mdu	576	8	of	of	IN
cord-014597-66vd2mdu	576	9	an	an	DT
cord-014597-66vd2mdu	576	10	antibody	antibody	NN
cord-014597-66vd2mdu	576	11	Fab	Fab	NNP
cord-014597-66vd2mdu	576	12	fragment	fragment	NN
cord-014597-66vd2mdu	576	13	through	through	IN
cord-014597-66vd2mdu	576	14	transient	transient	JJ
cord-014597-66vd2mdu	576	15	gene	gene	NN
cord-014597-66vd2mdu	576	16	expression	expression	NN
cord-014597-66vd2mdu	576	17	in	in	IN
cord-014597-66vd2mdu	576	18	lepidopteran	lepidopteran	JJ
cord-014597-66vd2mdu	576	19	insect	insect	NN
cord-014597-66vd2mdu	576	20	cells	cell	NNS
cord-014597-66vd2mdu	576	21	was	be	VBD
cord-014597-66vd2mdu	576	22	examined	examine	VBN
cord-014597-66vd2mdu	576	23	.	.	.
cord-014597-66vd2mdu	577	1	The	the	DT
cord-014597-66vd2mdu	577	2	DNA	dna	NN
cord-014597-66vd2mdu	577	3	fragments	fragment	NNS
cord-014597-66vd2mdu	577	4	encoding	encode	VBG
cord-014597-66vd2mdu	577	5	the	the	DT
cord-014597-66vd2mdu	577	6	heavy	heavy	JJ
cord-014597-66vd2mdu	577	7	chain	chain	NN
cord-014597-66vd2mdu	577	8	(	(	-LRB-
cord-014597-66vd2mdu	577	9	Hc	Hc	NNP
cord-014597-66vd2mdu	577	10	)	)	-RRB-
cord-014597-66vd2mdu	577	11	and	and	CC
cord-014597-66vd2mdu	578	1	light	light	JJ
cord-014597-66vd2mdu	578	2	chain	chain	NN
cord-014597-66vd2mdu	578	3	(	(	-LRB-
cord-014597-66vd2mdu	578	4	Lc	Lc	NNP
cord-014597-66vd2mdu	578	5	)	)	-RRB-
cord-014597-66vd2mdu	578	6	genes	gene	NNS
cord-014597-66vd2mdu	578	7	of	of	IN
cord-014597-66vd2mdu	578	8	an	an	DT
cord-014597-66vd2mdu	578	9	Fab	Fab	NNP
cord-014597-66vd2mdu	578	10	fragment	fragment	NN
cord-014597-66vd2mdu	578	11	of	of	IN
cord-014597-66vd2mdu	578	12	mouse	mouse	NN
cord-014597-66vd2mdu	578	13	anti	anti	JJ
cord-014597-66vd2mdu	578	14	-	-	JJ
cord-014597-66vd2mdu	578	15	bovine	bovine	JJ
cord-014597-66vd2mdu	578	16	RNaseA	rnasea	NN
cord-014597-66vd2mdu	578	17	[	[	-LRB-
cord-014597-66vd2mdu	578	18	3	3	CD
cord-014597-66vd2mdu	578	19	]	]	-RRB-
cord-014597-66vd2mdu	578	20	were	be	VBD
cord-014597-66vd2mdu	578	21	respectively	respectively	RB
cord-014597-66vd2mdu	578	22	cloned	clone	VBN
cord-014597-66vd2mdu	578	23	into	into	IN
cord-014597-66vd2mdu	578	24	the	the	DT
cord-014597-66vd2mdu	578	25	plasmid	plasmid	NN
cord-014597-66vd2mdu	578	26	vector	vector	NN
cord-014597-66vd2mdu	578	27	pIHAneo	pIHAneo	NNP
cord-014597-66vd2mdu	578	28	,	,	,
cord-014597-66vd2mdu	578	29	which	which	WDT
cord-014597-66vd2mdu	578	30	contained	contain	VBD
cord-014597-66vd2mdu	578	31	the	the	DT
cord-014597-66vd2mdu	578	32	Bombyx	Bombyx	NNP
cord-014597-66vd2mdu	578	33	mori	mori	NN
cord-014597-66vd2mdu	578	34	actin	actin	NN
cord-014597-66vd2mdu	578	35	promoter	promoter	NN
cord-014597-66vd2mdu	578	36	downstream	downstream	RB
cord-014597-66vd2mdu	578	37	of	of	IN
cord-014597-66vd2mdu	578	38	the	the	DT
cord-014597-66vd2mdu	578	39	B.	B.	NNP
cord-014597-66vd2mdu	578	40	mori	mori	NNP
cord-014597-66vd2mdu	578	41	nucleopolyhedrovirus	nucleopolyhedrovirus	NNP
cord-014597-66vd2mdu	578	42	(	(	-LRB-
cord-014597-66vd2mdu	578	43	BmNPV	BmNPV	NNP
cord-014597-66vd2mdu	578	44	)	)	-RRB-
cord-014597-66vd2mdu	579	1	IE-1	IE-1	NNP
cord-014597-66vd2mdu	580	1	transactivator	transactivator	NNP
cord-014597-66vd2mdu	580	2	and	and	CC
cord-014597-66vd2mdu	580	3	the	the	DT
cord-014597-66vd2mdu	580	4	BmNPV	BmNPV	NNP
cord-014597-66vd2mdu	580	5	HR3	HR3	NNP
cord-014597-66vd2mdu	580	6	enhancer	enhancer	NN
cord-014597-66vd2mdu	580	7	for	for	IN
cord-014597-66vd2mdu	580	8	high	high	JJ
cord-014597-66vd2mdu	580	9	-	-	HYPH
cord-014597-66vd2mdu	580	10	level	level	NN
cord-014597-66vd2mdu	580	11	expression	expression	NN
cord-014597-66vd2mdu	580	12	[	[	-LRB-
cord-014597-66vd2mdu	580	13	4	4	CD
cord-014597-66vd2mdu	580	14	]	]	-RRB-
cord-014597-66vd2mdu	580	15	.	.	.
cord-014597-66vd2mdu	581	1	Trichoplusia	Trichoplusia	NNP
cord-014597-66vd2mdu	581	2	ni	ni	NNP
cord-014597-66vd2mdu	582	1	BTI	BTI	NNP
cord-014597-66vd2mdu	582	2	-	-	HYPH
cord-014597-66vd2mdu	582	3	TN-5B1	TN-5B1	NNP
cord-014597-66vd2mdu	582	4	-	-	HYPH
cord-014597-66vd2mdu	582	5	4	4	CD
cord-014597-66vd2mdu	583	1	(	(	-LRB-
cord-014597-66vd2mdu	583	2	High	High	NNP
cord-014597-66vd2mdu	583	3	Five	five	CD
cord-014597-66vd2mdu	583	4	)	)	-RRB-
cord-014597-66vd2mdu	583	5	cells	cell	NNS
cord-014597-66vd2mdu	583	6	were	be	VBD
cord-014597-66vd2mdu	583	7	co	co	JJ
cord-014597-66vd2mdu	583	8	-	-	VBN
cord-014597-66vd2mdu	583	9	transfected	transfecte	VBN
cord-014597-66vd2mdu	583	10	with	with	IN
cord-014597-66vd2mdu	583	11	the	the	DT
cord-014597-66vd2mdu	583	12	resultant	resultant	JJ
cord-014597-66vd2mdu	583	13	plasmid	plasmid	NN
cord-014597-66vd2mdu	583	14	vectors	vector	NNS
cord-014597-66vd2mdu	583	15	using	use	VBG
cord-014597-66vd2mdu	583	16	linear	linear	JJ
cord-014597-66vd2mdu	583	17	polyethyleneimine	polyethyleneimine	NNP
cord-014597-66vd2mdu	583	18	(	(	-LRB-
cord-014597-66vd2mdu	583	19	PEI	PEI	NNP
cord-014597-66vd2mdu	583	20	;	;	:
cord-014597-66vd2mdu	583	21	Mw	Mw	NNP
cord-014597-66vd2mdu	583	22	40,000	40,000	CD
cord-014597-66vd2mdu	583	23	)	)	-RRB-
cord-014597-66vd2mdu	583	24	.	.	.
cord-014597-66vd2mdu	584	1	Before	before	IN
cord-014597-66vd2mdu	584	2	transfection	transfection	NN
cord-014597-66vd2mdu	584	3	,	,	,
cord-014597-66vd2mdu	584	4	the	the	DT
cord-014597-66vd2mdu	584	5	plasmids	plasmid	NNS
cord-014597-66vd2mdu	584	6	and	and	CC
cord-014597-66vd2mdu	584	7	PEI	PEI	NNP
cord-014597-66vd2mdu	584	8	were	be	VBD
cord-014597-66vd2mdu	584	9	prepared	prepare	VBN
cord-014597-66vd2mdu	584	10	in	in	IN
cord-014597-66vd2mdu	584	11	150	150	CD
cord-014597-66vd2mdu	584	12	mM	mm	CD
cord-014597-66vd2mdu	584	13	NaCl	nacl	NN
cord-014597-66vd2mdu	584	14	,	,	,
cord-014597-66vd2mdu	584	15	pH	pH	NNP
cord-014597-66vd2mdu	584	16	7.0	7.0	CD
cord-014597-66vd2mdu	584	17	and	and	CC
cord-014597-66vd2mdu	584	18	incubated	incubate	VBN
cord-014597-66vd2mdu	584	19	at	at	IN
cord-014597-66vd2mdu	584	20	room	room	NN
cord-014597-66vd2mdu	584	21	temperature	temperature	NN
cord-014597-66vd2mdu	584	22	for	for	IN
cord-014597-66vd2mdu	584	23	5	5	CD
cord-014597-66vd2mdu	584	24	min	min	NN
cord-014597-66vd2mdu	584	25	.	.	.
cord-014597-66vd2mdu	585	1	When	when	WRB
cord-014597-66vd2mdu	585	2	the	the	DT
cord-014597-66vd2mdu	585	3	transfection	transfection	NN
cord-014597-66vd2mdu	585	4	efficiency	efficiency	NN
cord-014597-66vd2mdu	585	5	was	be	VBD
cord-014597-66vd2mdu	585	6	checked	check	VBN
cord-014597-66vd2mdu	585	7	,	,	,
cord-014597-66vd2mdu	585	8	a	a	DT
cord-014597-66vd2mdu	585	9	plasmid	plasmid	NN
cord-014597-66vd2mdu	585	10	vector	vector	NN
cord-014597-66vd2mdu	585	11	encoding	encoding	NN
cord-014597-66vd2mdu	586	1	the	the	DT
cord-014597-66vd2mdu	586	2	enhanced	enhanced	JJ
cord-014597-66vd2mdu	586	3	green	green	JJ
cord-014597-66vd2mdu	586	4	fluorescent	fluorescent	NN
cord-014597-66vd2mdu	586	5	protein	protein	NN
cord-014597-66vd2mdu	586	6	(	(	-LRB-
cord-014597-66vd2mdu	586	7	EGFP	EGFP	NNP
cord-014597-66vd2mdu	586	8	)	)	-RRB-
cord-014597-66vd2mdu	586	9	gene	gene	NN
cord-014597-66vd2mdu	586	10	was	be	VBD
cord-014597-66vd2mdu	586	11	also	also	RB
cord-014597-66vd2mdu	586	12	co	co	VBN
cord-014597-66vd2mdu	586	13	-	-	VBN
cord-014597-66vd2mdu	586	14	transfected	transfecte	VBN
cord-014597-66vd2mdu	586	15	.	.	.
cord-014597-66vd2mdu	587	1	Transfected	transfected	JJ
cord-014597-66vd2mdu	587	2	cells	cell	NNS
cord-014597-66vd2mdu	587	3	were	be	VBD
cord-014597-66vd2mdu	587	4	incubated	incubate	VBN
cord-014597-66vd2mdu	587	5	with	with	IN
cord-014597-66vd2mdu	587	6	a	a	DT
cord-014597-66vd2mdu	587	7	serum	serum	NN
cord-014597-66vd2mdu	587	8	-	-	HYPH
cord-014597-66vd2mdu	587	9	free	free	JJ
cord-014597-66vd2mdu	587	10	medium	medium	NN
cord-014597-66vd2mdu	587	11	in	in	IN
cord-014597-66vd2mdu	587	12	a	a	DT
cord-014597-66vd2mdu	587	13	static	static	JJ
cord-014597-66vd2mdu	587	14	or	or	CC
cord-014597-66vd2mdu	587	15	shake	shake	NN
cord-014597-66vd2mdu	587	16	-	-	HYPH
cord-014597-66vd2mdu	587	17	flask	flask	NN
cord-014597-66vd2mdu	587	18	culture	culture	NN
cord-014597-66vd2mdu	587	19	.	.	.
cord-014597-66vd2mdu	588	1	Culture	culture	NN
cord-014597-66vd2mdu	588	2	supernatants	supernatant	NNS
cord-014597-66vd2mdu	588	3	were	be	VBD
cord-014597-66vd2mdu	588	4	analysed	analyse	VBN
cord-014597-66vd2mdu	588	5	by	by	IN
cord-014597-66vd2mdu	588	6	western	western	JJ
cord-014597-66vd2mdu	588	7	blotting	blotting	NN
cord-014597-66vd2mdu	588	8	and	and	CC
cord-014597-66vd2mdu	588	9	enzyme	enzyme	NN
cord-014597-66vd2mdu	588	10	-	-	HYPH
cord-014597-66vd2mdu	588	11	linked	link	VBN
cord-014597-66vd2mdu	588	12	immunosorbent	immunosorbent	JJ
cord-014597-66vd2mdu	588	13	assay	assay	NN
cord-014597-66vd2mdu	588	14	(	(	-LRB-
cord-014597-66vd2mdu	588	15	ELISA	ELISA	NNP
cord-014597-66vd2mdu	588	16	)	)	-RRB-
cord-014597-66vd2mdu	588	17	.	.	.
cord-014597-66vd2mdu	589	1	The	the	DT
cord-014597-66vd2mdu	589	2	numbers	number	NNS
cord-014597-66vd2mdu	589	3	of	of	IN
cord-014597-66vd2mdu	589	4	green	green	JJ
cord-014597-66vd2mdu	589	5	fluorescent	fluorescent	NN
cord-014597-66vd2mdu	589	6	cells	cell	NNS
cord-014597-66vd2mdu	589	7	and	and	CC
cord-014597-66vd2mdu	589	8	total	total	JJ
cord-014597-66vd2mdu	589	9	cells	cell	NNS
cord-014597-66vd2mdu	589	10	in	in	IN
cord-014597-66vd2mdu	589	11	culture	culture	NN
cord-014597-66vd2mdu	589	12	broth	broth	NN
cord-014597-66vd2mdu	589	13	was	be	VBD
cord-014597-66vd2mdu	589	14	determined	determine	VBN
cord-014597-66vd2mdu	589	15	using	use	VBG
cord-014597-66vd2mdu	589	16	a	a	DT
cord-014597-66vd2mdu	589	17	flow	flow	NN
cord-014597-66vd2mdu	589	18	cytometer	cytometer	NN
cord-014597-66vd2mdu	589	19	.	.	.
cord-014597-66vd2mdu	590	1	Western	western	JJ
cord-014597-66vd2mdu	590	2	blot	blot	NN
cord-014597-66vd2mdu	590	3	analysis	analysis	NN
cord-014597-66vd2mdu	590	4	and	and	CC
cord-014597-66vd2mdu	590	5	ELISA	ELISA	NNP
cord-014597-66vd2mdu	590	6	of	of	IN
cord-014597-66vd2mdu	590	7	culture	culture	NN
cord-014597-66vd2mdu	590	8	supernatants	supernatant	NNS
cord-014597-66vd2mdu	590	9	showed	show	VBD
cord-014597-66vd2mdu	590	10	that	that	IN
cord-014597-66vd2mdu	590	11	transfected	transfecte	VBN
cord-014597-66vd2mdu	590	12	High	High	NNP
cord-014597-66vd2mdu	590	13	Five	five	CD
cord-014597-66vd2mdu	590	14	cells	cell	NNS
cord-014597-66vd2mdu	590	15	secreted	secrete	VBD
cord-014597-66vd2mdu	590	16	the	the	DT
cord-014597-66vd2mdu	590	17	Fab	Fab	NNP
cord-014597-66vd2mdu	590	18	fragment	fragment	NN
cord-014597-66vd2mdu	590	19	with	with	IN
cord-014597-66vd2mdu	590	20	antigenbinding	antigenbinding	NN
cord-014597-66vd2mdu	590	21	activity	activity	NN
cord-014597-66vd2mdu	590	22	.	.	.
cord-014597-66vd2mdu	591	1	In	in	IN
cord-014597-66vd2mdu	591	2	static	static	JJ
cord-014597-66vd2mdu	591	3	cultures	culture	NNS
cord-014597-66vd2mdu	591	4	,	,	,
cord-014597-66vd2mdu	591	5	transfection	transfection	NN
cord-014597-66vd2mdu	591	6	and	and	CC
cord-014597-66vd2mdu	591	7	culture	culture	NN
cord-014597-66vd2mdu	591	8	conditions	condition	NNS
cord-014597-66vd2mdu	591	9	,	,	,
cord-014597-66vd2mdu	591	10	such	such	JJ
cord-014597-66vd2mdu	591	11	as	as	IN
cord-014597-66vd2mdu	591	12	Hc	Hc	NNP
cord-014597-66vd2mdu	591	13	:	:	:
cord-014597-66vd2mdu	592	1	Lc	Lc	NNP
cord-014597-66vd2mdu	592	2	gene	gene	NN
cord-014597-66vd2mdu	592	3	ratio	ratio	NN
cord-014597-66vd2mdu	592	4	,	,	,
cord-014597-66vd2mdu	592	5	a	a	DT
cord-014597-66vd2mdu	592	6	serum	serum	NN
cord-014597-66vd2mdu	592	7	-	-	HYPH
cord-014597-66vd2mdu	592	8	free	free	JJ
cord-014597-66vd2mdu	592	9	medium	medium	NN
cord-014597-66vd2mdu	592	10	,	,	,
cord-014597-66vd2mdu	592	11	DNA	DNA	NNP
cord-014597-66vd2mdu	592	12	:	:	:
cord-014597-66vd2mdu	592	13	PEI	PEI	NNP
cord-014597-66vd2mdu	592	14	ratio	ratio	NN
cord-014597-66vd2mdu	592	15	,	,	,
cord-014597-66vd2mdu	592	16	and	and	CC
cord-014597-66vd2mdu	592	17	DNA	dna	NN
cord-014597-66vd2mdu	592	18	amount	amount	NN
cord-014597-66vd2mdu	592	19	per	per	IN
cord-014597-66vd2mdu	592	20	cell	cell	NN
cord-014597-66vd2mdu	592	21	,	,	,
cord-014597-66vd2mdu	592	22	were	be	VBD
cord-014597-66vd2mdu	592	23	successfully	successfully	RB
cord-014597-66vd2mdu	592	24	optimized	optimize	VBN
cord-014597-66vd2mdu	592	25	by	by	IN
cord-014597-66vd2mdu	592	26	flow	flow	NN
cord-014597-66vd2mdu	592	27	cytometry	cytometry	NN
cord-014597-66vd2mdu	592	28	of	of	IN
cord-014597-66vd2mdu	592	29	EGFP	EGFP	NNP
cord-014597-66vd2mdu	592	30	expression	expression	NN
cord-014597-66vd2mdu	592	31	in	in	IN
cord-014597-66vd2mdu	592	32	transfected	transfected	JJ
cord-014597-66vd2mdu	592	33	cells	cell	NNS
cord-014597-66vd2mdu	592	34	and	and	CC
cord-014597-66vd2mdu	592	35	the	the	DT
cord-014597-66vd2mdu	592	36	yield	yield	NN
cord-014597-66vd2mdu	592	37	of	of	IN
cord-014597-66vd2mdu	592	38	the	the	DT
cord-014597-66vd2mdu	592	39	secreted	secrete	VBN
cord-014597-66vd2mdu	592	40	Fab	Fab	NNP
cord-014597-66vd2mdu	592	41	fragment	fragment	NN
cord-014597-66vd2mdu	592	42	measured	measure	VBN
cord-014597-66vd2mdu	592	43	by	by	IN
cord-014597-66vd2mdu	592	44	ELISA	ELISA	NNP
cord-014597-66vd2mdu	592	45	.	.	.
cord-014597-66vd2mdu	593	1	The	the	DT
cord-014597-66vd2mdu	593	2	effects	effect	NNS
cord-014597-66vd2mdu	593	3	of	of	IN
cord-014597-66vd2mdu	593	4	culture	culture	NN
cord-014597-66vd2mdu	593	5	temperature	temperature	NN
cord-014597-66vd2mdu	593	6	and	and	CC
cord-014597-66vd2mdu	593	7	initial	initial	JJ
cord-014597-66vd2mdu	593	8	cell	cell	NN
cord-014597-66vd2mdu	593	9	density	density	NN
cord-014597-66vd2mdu	593	10	were	be	VBD
cord-014597-66vd2mdu	593	11	also	also	RB
cord-014597-66vd2mdu	593	12	examined	examine	VBN
cord-014597-66vd2mdu	593	13	by	by	IN
cord-014597-66vd2mdu	593	14	comparing	compare	VBG
cord-014597-66vd2mdu	593	15	the	the	DT
cord-014597-66vd2mdu	593	16	cell	cell	NN
cord-014597-66vd2mdu	593	17	growth	growth	NN
cord-014597-66vd2mdu	593	18	and	and	CC
cord-014597-66vd2mdu	593	19	the	the	DT
cord-014597-66vd2mdu	593	20	production	production	NN
cord-014597-66vd2mdu	593	21	of	of	IN
cord-014597-66vd2mdu	593	22	Fab	fab	JJ
cord-014597-66vd2mdu	593	23	fragments	fragment	NNS
cord-014597-66vd2mdu	593	24	in	in	IN
cord-014597-66vd2mdu	593	25	shake	shake	NN
cord-014597-66vd2mdu	593	26	-	-	HYPH
cord-014597-66vd2mdu	593	27	flask	flask	NN
cord-014597-66vd2mdu	593	28	cultures	culture	NNS
cord-014597-66vd2mdu	593	29	.	.	.
cord-014597-66vd2mdu	594	1	Under	under	IN
cord-014597-66vd2mdu	594	2	optimal	optimal	JJ
cord-014597-66vd2mdu	594	3	conditions	condition	NNS
cord-014597-66vd2mdu	594	4	(	(	-LRB-
cord-014597-66vd2mdu	594	5	medium	medium	NN
cord-014597-66vd2mdu	594	6	,	,	,
cord-014597-66vd2mdu	594	7	PSFM	PSFM	NNP
cord-014597-66vd2mdu	594	8	-	-	HYPH
cord-014597-66vd2mdu	594	9	J1	J1	NNP
cord-014597-66vd2mdu	594	10	(	(	-LRB-
cord-014597-66vd2mdu	594	11	Wako	Wako	NNP
cord-014597-66vd2mdu	594	12	Pure	Pure	NNP
cord-014597-66vd2mdu	594	13	Chemical	Chemical	NNP
cord-014597-66vd2mdu	594	14	Industries	Industries	NNPS
cord-014597-66vd2mdu	594	15	,	,	,
cord-014597-66vd2mdu	594	16	Japan	Japan	NNP
cord-014597-66vd2mdu	594	17	)	)	-RRB-
cord-014597-66vd2mdu	595	1	;	;	:
cord-014597-66vd2mdu	595	2	Hc	Hc	NNP
cord-014597-66vd2mdu	595	3	:	:	:
cord-014597-66vd2mdu	595	4	Lc	Lc	NNP
cord-014597-66vd2mdu	595	5	gene	gene	NN
cord-014597-66vd2mdu	595	6	ratio	ratio	NN
cord-014597-66vd2mdu	595	7	,	,	,
cord-014597-66vd2mdu	595	8	3:7	3:7	CD
cord-014597-66vd2mdu	595	9	;	;	:
cord-014597-66vd2mdu	595	10	DNA	DNA	NNP
cord-014597-66vd2mdu	595	11	,	,	,
cord-014597-66vd2mdu	595	12	5	5	CD
cord-014597-66vd2mdu	595	13	μg/(10	μg/(10	CD
cord-014597-66vd2mdu	595	14	6	6	CD
cord-014597-66vd2mdu	595	15	cells	cell	NNS
cord-014597-66vd2mdu	595	16	)	)	-RRB-
cord-014597-66vd2mdu	595	17	;	;	:
cord-014597-66vd2mdu	595	18	PEI	PEI	NNP
cord-014597-66vd2mdu	595	19	,	,	,
cord-014597-66vd2mdu	595	20	10	10	CD
cord-014597-66vd2mdu	595	21	μg/(10	μg/(10	CD
cord-014597-66vd2mdu	595	22	6	6	CD
cord-014597-66vd2mdu	595	23	cells	cell	NNS
cord-014597-66vd2mdu	595	24	)	)	-RRB-
cord-014597-66vd2mdu	595	25	;	;	,
cord-014597-66vd2mdu	595	26	initial	initial	JJ
cord-014597-66vd2mdu	595	27	cell	cell	NN
cord-014597-66vd2mdu	595	28	density	density	NN
cord-014597-66vd2mdu	595	29	,	,	,
cord-014597-66vd2mdu	595	30	1	1	CD
cord-014597-66vd2mdu	595	31	x	x	SYM
cord-014597-66vd2mdu	595	32	10	10	CD
cord-014597-66vd2mdu	595	33	6	6	CD
cord-014597-66vd2mdu	595	34	cells	cell	NNS
cord-014597-66vd2mdu	595	35	/	/	SYM
cord-014597-66vd2mdu	595	36	cm	cm	NN
cord-014597-66vd2mdu	595	37	3	3	CD
cord-014597-66vd2mdu	595	38	;	;	:
cord-014597-66vd2mdu	595	39	temperature	temperature	NN
cord-014597-66vd2mdu	595	40	,	,	,
cord-014597-66vd2mdu	595	41	24	24	CD
cord-014597-66vd2mdu	595	42	°	°	NN
cord-014597-66vd2mdu	595	43	C	C	NNP
cord-014597-66vd2mdu	595	44	)	)	-RRB-
cord-014597-66vd2mdu	595	45	,	,	,
cord-014597-66vd2mdu	595	46	the	the	DT
cord-014597-66vd2mdu	595	47	yield	yield	NN
cord-014597-66vd2mdu	595	48	of	of	IN
cord-014597-66vd2mdu	595	49	more	more	JJR
cord-014597-66vd2mdu	595	50	than	than	IN
cord-014597-66vd2mdu	595	51	100	100	CD
cord-014597-66vd2mdu	595	52	mg	mg	NNP
cord-014597-66vd2mdu	595	53	/	/	SYM
cord-014597-66vd2mdu	595	54	L	L	NNP
cord-014597-66vd2mdu	595	55	of	of	IN
cord-014597-66vd2mdu	595	56	Fab	Fab	NNP
cord-014597-66vd2mdu	595	57	fragment	fragment	NN
cord-014597-66vd2mdu	595	58	was	be	VBD
cord-014597-66vd2mdu	595	59	achieved	achieve	VBN
cord-014597-66vd2mdu	595	60	in	in	IN
cord-014597-66vd2mdu	595	61	5	5	CD
cord-014597-66vd2mdu	595	62	days	day	NNS
cord-014597-66vd2mdu	595	63	in	in	IN
cord-014597-66vd2mdu	595	64	a	a	DT
cord-014597-66vd2mdu	595	65	shake	shake	NN
cord-014597-66vd2mdu	595	66	-	-	HYPH
cord-014597-66vd2mdu	595	67	flask	flask	NN
cord-014597-66vd2mdu	595	68	culture	culture	NN
cord-014597-66vd2mdu	595	69	(	(	-LRB-
cord-014597-66vd2mdu	595	70	Fig	Fig	NNP
cord-014597-66vd2mdu	595	71	.	.	.
cord-014597-66vd2mdu	595	72	1	1	CD
cord-014597-66vd2mdu	595	73	)	)	-RRB-
cord-014597-66vd2mdu	595	74	.	.	.
cord-014597-66vd2mdu	596	1	Transfection	Transfection	NNP
cord-014597-66vd2mdu	596	2	did	do	VBD
cord-014597-66vd2mdu	596	3	not	not	RB
cord-014597-66vd2mdu	596	4	significantly	significantly	RB
cord-014597-66vd2mdu	596	5	affect	affect	VB
cord-014597-66vd2mdu	596	6	the	the	DT
cord-014597-66vd2mdu	596	7	growth	growth	NN
cord-014597-66vd2mdu	596	8	of	of	IN
cord-014597-66vd2mdu	596	9	High	High	NNP
cord-014597-66vd2mdu	596	10	Five	five	CD
cord-014597-66vd2mdu	596	11	cells	cell	NNS
cord-014597-66vd2mdu	596	12	as	as	IN
cord-014597-66vd2mdu	596	13	compared	compare	VBN
cord-014597-66vd2mdu	596	14	with	with	IN
cord-014597-66vd2mdu	596	15	untransfected	untransfected	JJ
cord-014597-66vd2mdu	596	16	cells	cell	NNS
cord-014597-66vd2mdu	596	17	.	.	.
cord-014597-66vd2mdu	597	1	Transient	transient	JJ
cord-014597-66vd2mdu	597	2	gene	gene	NN
cord-014597-66vd2mdu	597	3	expression	expression	NN
cord-014597-66vd2mdu	597	4	using	use	VBG
cord-014597-66vd2mdu	597	5	insect	insect	NN
cord-014597-66vd2mdu	597	6	cells	cell	NNS
cord-014597-66vd2mdu	597	7	may	may	MD
cord-014597-66vd2mdu	597	8	offer	offer	VB
cord-014597-66vd2mdu	597	9	a	a	DT
cord-014597-66vd2mdu	597	10	promising	promising	JJ
cord-014597-66vd2mdu	597	11	approach	approach	NN
cord-014597-66vd2mdu	597	12	to	to	IN
cord-014597-66vd2mdu	597	13	high	high	JJ
cord-014597-66vd2mdu	597	14	-	-	HYPH
cord-014597-66vd2mdu	597	15	throughput	throughput	NN
cord-014597-66vd2mdu	597	16	production	production	NN
cord-014597-66vd2mdu	597	17	of	of	IN
cord-014597-66vd2mdu	597	18	candidate	candidate	NN
cord-014597-66vd2mdu	597	19	proteins	protein	NNS
cord-014597-66vd2mdu	597	20	for	for	IN
cord-014597-66vd2mdu	597	21	the	the	DT
cord-014597-66vd2mdu	597	22	development	development	NN
cord-014597-66vd2mdu	597	23	of	of	IN
cord-014597-66vd2mdu	597	24	biologics	biologic	NNS
cord-014597-66vd2mdu	597	25	.	.	.
cord-014597-66vd2mdu	597	26	 	 	_SP
cord-014597-66vd2mdu	598	1	The	the	DT
cord-014597-66vd2mdu	598	2	increasing	increase	VBG
cord-014597-66vd2mdu	598	3	demand	demand	NN
cord-014597-66vd2mdu	598	4	for	for	IN
cord-014597-66vd2mdu	598	5	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	598	6	produced	produce	VBN
cord-014597-66vd2mdu	598	7	in	in	IN
cord-014597-66vd2mdu	598	8	mammalian	mammalian	JJ
cord-014597-66vd2mdu	598	9	cells	cell	NNS
cord-014597-66vd2mdu	598	10	has	have	VBZ
cord-014597-66vd2mdu	598	11	led	lead	VBN
cord-014597-66vd2mdu	598	12	industries	industry	NNS
cord-014597-66vd2mdu	598	13	to	to	TO
cord-014597-66vd2mdu	598	14	increase	increase	VB
cord-014597-66vd2mdu	598	15	volumetric	volumetric	JJ
cord-014597-66vd2mdu	598	16	productivity	productivity	NN
cord-014597-66vd2mdu	598	17	of	of	IN
cord-014597-66vd2mdu	598	18	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	598	19	through	through	IN
cord-014597-66vd2mdu	598	20	different	different	JJ
cord-014597-66vd2mdu	598	21	strategies	strategy	NNS
cord-014597-66vd2mdu	598	22	[	[	-LRB-
cord-014597-66vd2mdu	598	23	1	1	CD
cord-014597-66vd2mdu	598	24	,	,	,
cord-014597-66vd2mdu	598	25	2	2	CD
cord-014597-66vd2mdu	598	26	,	,	,
cord-014597-66vd2mdu	598	27	3	3	CD
cord-014597-66vd2mdu	598	28	]	]	-RRB-
cord-014597-66vd2mdu	598	29	.	.	.
cord-014597-66vd2mdu	599	1	In	in	IN
cord-014597-66vd2mdu	599	2	this	this	DT
cord-014597-66vd2mdu	599	3	context	context	NN
cord-014597-66vd2mdu	599	4	,	,	,
cord-014597-66vd2mdu	599	5	fedbatch	fedbatch	NN
cord-014597-66vd2mdu	599	6	and	and	CC
cord-014597-66vd2mdu	599	7	perfusion	perfusion	NN
cord-014597-66vd2mdu	599	8	cultures	culture	NNS
cord-014597-66vd2mdu	599	9	have	have	VBP
cord-014597-66vd2mdu	599	10	attracted	attract	VBN
cord-014597-66vd2mdu	599	11	more	more	JJR
cord-014597-66vd2mdu	599	12	interest	interest	NN
cord-014597-66vd2mdu	599	13	than	than	IN
cord-014597-66vd2mdu	599	14	conventional	conventional	JJ
cord-014597-66vd2mdu	599	15	batch	batch	NN
cord-014597-66vd2mdu	599	16	processes	process	NNS
cord-014597-66vd2mdu	599	17	.	.	.
cord-014597-66vd2mdu	600	1	The	the	DT
cord-014597-66vd2mdu	600	2	efficient	efficient	JJ
cord-014597-66vd2mdu	600	3	application	application	NN
cord-014597-66vd2mdu	600	4	of	of	IN
cord-014597-66vd2mdu	600	5	such	such	JJ
cord-014597-66vd2mdu	600	6	alternative	alternative	JJ
cord-014597-66vd2mdu	600	7	processes	process	NNS
cord-014597-66vd2mdu	600	8	requires	require	VBZ
cord-014597-66vd2mdu	600	9	the	the	DT
cord-014597-66vd2mdu	600	10	availability	availability	NN
cord-014597-66vd2mdu	600	11	of	of	IN
cord-014597-66vd2mdu	600	12	reliable	reliable	JJ
cord-014597-66vd2mdu	600	13	on	on	IN
cord-014597-66vd2mdu	600	14	-	-	HYPH
cord-014597-66vd2mdu	600	15	line	line	NN
cord-014597-66vd2mdu	600	16	measuring	measuring	NN
cord-014597-66vd2mdu	600	17	tools	tool	NNS
cord-014597-66vd2mdu	600	18	for	for	IN
cord-014597-66vd2mdu	600	19	cell	cell	NN
cord-014597-66vd2mdu	600	20	density	density	NN
cord-014597-66vd2mdu	600	21	and	and	CC
cord-014597-66vd2mdu	600	22	cell	cell	NN
cord-014597-66vd2mdu	600	23	metabolic	metabolic	JJ
cord-014597-66vd2mdu	600	24	activity	activity	NN
cord-014597-66vd2mdu	600	25	estimation	estimation	NN
cord-014597-66vd2mdu	600	26	[	[	-LRB-
cord-014597-66vd2mdu	600	27	4	4	CD
cord-014597-66vd2mdu	600	28	]	]	-RRB-
cord-014597-66vd2mdu	600	29	.	.	.
cord-014597-66vd2mdu	601	1	The	the	DT
cord-014597-66vd2mdu	601	2	comparison	comparison	NN
cord-014597-66vd2mdu	601	3	of	of	IN
cord-014597-66vd2mdu	601	4	different	different	JJ
cord-014597-66vd2mdu	601	5	culture	culture	NN
cord-014597-66vd2mdu	601	6	strategies	strategy	NNS
cord-014597-66vd2mdu	601	7	for	for	IN
cord-014597-66vd2mdu	601	8	HEK293	HEK293	NNP
cord-014597-66vd2mdu	601	9	cell	cell	NN
cord-014597-66vd2mdu	601	10	line	line	NN
cord-014597-66vd2mdu	601	11	producing	produce	VBG
cord-014597-66vd2mdu	601	12	IFN	IFN	NNP
cord-014597-66vd2mdu	601	13	-	-	HYPH
cord-014597-66vd2mdu	601	14	γ	γ	NNP
cord-014597-66vd2mdu	601	15	are	be	VBP
cord-014597-66vd2mdu	601	16	presented	present	VBN
cord-014597-66vd2mdu	601	17	below	below	IN
cord-014597-66vd2mdu	601	18	:	:	:
cord-014597-66vd2mdu	601	19	batch	batch	NN
cord-014597-66vd2mdu	601	20	,	,	,
cord-014597-66vd2mdu	601	21	fortified	fortified	JJ
cord-014597-66vd2mdu	601	22	batch	batch	NN
cord-014597-66vd2mdu	601	23	and	and	CC
cord-014597-66vd2mdu	601	24	fed	fed	JJ
cord-014597-66vd2mdu	601	25	-	-	HYPH
cord-014597-66vd2mdu	601	26	batch	batch	NN
cord-014597-66vd2mdu	601	27	.	.	.
cord-014597-66vd2mdu	602	1	In	in	IN
cord-014597-66vd2mdu	602	2	this	this	DT
cord-014597-66vd2mdu	602	3	context	context	NN
cord-014597-66vd2mdu	602	4	,	,	,
cord-014597-66vd2mdu	602	5	a	a	DT
cord-014597-66vd2mdu	602	6	new	new	JJ
cord-014597-66vd2mdu	602	7	robust	robust	JJ
cord-014597-66vd2mdu	602	8	feeding	feeding	NN
cord-014597-66vd2mdu	602	9	strategy	strategy	NN
cord-014597-66vd2mdu	602	10	based	base	VBN
cord-014597-66vd2mdu	602	11	on	on	IN
cord-014597-66vd2mdu	602	12	the	the	DT
cord-014597-66vd2mdu	602	13	monitoring	monitoring	NN
cord-014597-66vd2mdu	602	14	of	of	IN
cord-014597-66vd2mdu	602	15	alkali	alkali	JJ
cord-014597-66vd2mdu	602	16	buffer	buffer	NN
cord-014597-66vd2mdu	602	17	addition	addition	NN
cord-014597-66vd2mdu	602	18	was	be	VBD
cord-014597-66vd2mdu	602	19	applied	apply	VBN
cord-014597-66vd2mdu	602	20	for	for	IN
cord-014597-66vd2mdu	602	21	the	the	DT
cord-014597-66vd2mdu	602	22	estimation	estimation	NN
cord-014597-66vd2mdu	602	23	of	of	IN
cord-014597-66vd2mdu	602	24	nutrient	nutrient	NN
cord-014597-66vd2mdu	602	25	requirements	requirement	NNS
cord-014597-66vd2mdu	602	26	.	.	.
cord-014597-66vd2mdu	603	1	This	this	DT
cord-014597-66vd2mdu	603	2	method	method	NN
cord-014597-66vd2mdu	603	3	allows	allow	VBZ
cord-014597-66vd2mdu	603	4	to	to	TO
cord-014597-66vd2mdu	603	5	increase	increase	VB
cord-014597-66vd2mdu	603	6	cell	cell	NN
cord-014597-66vd2mdu	603	7	density	density	NN
cord-014597-66vd2mdu	603	8	and	and	CC
cord-014597-66vd2mdu	603	9	product	product	NN
cord-014597-66vd2mdu	603	10	titer	titer	NN
cord-014597-66vd2mdu	603	11	compared	compare	VBN
cord-014597-66vd2mdu	603	12	with	with	IN
cord-014597-66vd2mdu	603	13	the	the	DT
cord-014597-66vd2mdu	603	14	other	other	JJ
cord-014597-66vd2mdu	603	15	strategies	strategy	NNS
cord-014597-66vd2mdu	603	16	assessed	assess	VBN
cord-014597-66vd2mdu	603	17	.	.	.
cord-014597-66vd2mdu	604	1	Three	three	CD
cord-014597-66vd2mdu	604	2	different	different	JJ
cord-014597-66vd2mdu	604	3	culture	culture	NN
cord-014597-66vd2mdu	604	4	strategies	strategy	NNS
cord-014597-66vd2mdu	604	5	were	be	VBD
cord-014597-66vd2mdu	604	6	carried	carry	VBN
cord-014597-66vd2mdu	604	7	out	out	RP
cord-014597-66vd2mdu	604	8	in	in	IN
cord-014597-66vd2mdu	604	9	2-litre	2-litre	CD
cord-014597-66vd2mdu	604	10	Biostat	Biostat	NNP
cord-014597-66vd2mdu	604	11	B	B	NNP
cord-014597-66vd2mdu	604	12	-	-	HYPH
cord-014597-66vd2mdu	604	13	DCU	DCU	NNP
cord-014597-66vd2mdu	604	14	II	II	NNP
cord-014597-66vd2mdu	604	15	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	604	16	.	.	.
cord-014597-66vd2mdu	605	1	First	first	RB
cord-014597-66vd2mdu	605	2	,	,	,
cord-014597-66vd2mdu	605	3	a	a	DT
cord-014597-66vd2mdu	605	4	reference	reference	NN
cord-014597-66vd2mdu	605	5	batch	batch	NN
cord-014597-66vd2mdu	605	6	and	and	CC
cord-014597-66vd2mdu	605	7	a	a	DT
cord-014597-66vd2mdu	605	8	batch	batch	NN
cord-014597-66vd2mdu	605	9	using	use	VBG
cord-014597-66vd2mdu	605	10	fortified	fortified	JJ
cord-014597-66vd2mdu	605	11	medium	medium	NN
cord-014597-66vd2mdu	605	12	(	(	-LRB-
cord-014597-66vd2mdu	605	13	nutrient	nutrient	NN
cord-014597-66vd2mdu	605	14	enriched	enrich	VBN
cord-014597-66vd2mdu	605	15	medium	medium	NN
cord-014597-66vd2mdu	605	16	)	)	-RRB-
cord-014597-66vd2mdu	605	17	were	be	VBD
cord-014597-66vd2mdu	605	18	run	run	VBN
cord-014597-66vd2mdu	605	19	and	and	CC
cord-014597-66vd2mdu	605	20	assessed	assess	VBN
cord-014597-66vd2mdu	605	21	in	in	IN
cord-014597-66vd2mdu	605	22	terms	term	NNS
cord-014597-66vd2mdu	605	23	of	of	IN
cord-014597-66vd2mdu	605	24	Viable	viable	JJ
cord-014597-66vd2mdu	605	25	Cell	cell	NN
cord-014597-66vd2mdu	605	26	Density	density	NN
cord-014597-66vd2mdu	605	27	(	(	-LRB-
cord-014597-66vd2mdu	605	28	VCD	vcd	NN
cord-014597-66vd2mdu	605	29	)	)	-RRB-
cord-014597-66vd2mdu	605	30	and	and	CC
cord-014597-66vd2mdu	605	31	product	product	NN
cord-014597-66vd2mdu	605	32	titer	titer	NN
cord-014597-66vd2mdu	605	33	,	,	,
cord-014597-66vd2mdu	605	34	and	and	CC
cord-014597-66vd2mdu	605	35	set	set	VBD
cord-014597-66vd2mdu	605	36	as	as	IN
cord-014597-66vd2mdu	605	37	initial	initial	JJ
cord-014597-66vd2mdu	605	38	references	reference	NNS
cord-014597-66vd2mdu	605	39	.	.	.
cord-014597-66vd2mdu	606	1	Then	then	RB
cord-014597-66vd2mdu	606	2	,	,	,
cord-014597-66vd2mdu	606	3	a	a	DT
cord-014597-66vd2mdu	606	4	fed	feed	VBN
cord-014597-66vd2mdu	606	5	-	-	HYPH
cord-014597-66vd2mdu	606	6	batch	batch	NN
cord-014597-66vd2mdu	606	7	was	be	VBD
cord-014597-66vd2mdu	606	8	performed	perform	VBN
cord-014597-66vd2mdu	606	9	applying	apply	VBG
cord-014597-66vd2mdu	606	10	a	a	DT
cord-014597-66vd2mdu	606	11	feeding	feeding	NN
cord-014597-66vd2mdu	606	12	strategy	strategy	NN
cord-014597-66vd2mdu	606	13	based	base	VBN
cord-014597-66vd2mdu	606	14	on	on	IN
cord-014597-66vd2mdu	606	15	the	the	DT
cord-014597-66vd2mdu	606	16	nutrient	nutrient	NN
cord-014597-66vd2mdu	606	17	requirements	requirement	NNS
cord-014597-66vd2mdu	606	18	estimation	estimation	NN
cord-014597-66vd2mdu	606	19	by	by	IN
cord-014597-66vd2mdu	606	20	monitoring	monitor	VBG
cord-014597-66vd2mdu	606	21	the	the	DT
cord-014597-66vd2mdu	606	22	alkali	alkali	JJ
cord-014597-66vd2mdu	606	23	buffer	buffer	NN
cord-014597-66vd2mdu	606	24	addition	addition	NN
cord-014597-66vd2mdu	606	25	used	use	VBN
cord-014597-66vd2mdu	606	26	for	for	IN
cord-014597-66vd2mdu	606	27	the	the	DT
cord-014597-66vd2mdu	606	28	control	control	NN
cord-014597-66vd2mdu	606	29	of	of	IN
cord-014597-66vd2mdu	606	30	pH.	ph.	NN
cord-014597-66vd2mdu	606	31	Results	Results	NNP
cord-014597-66vd2mdu	606	32	VCD	VCD	NNP
cord-014597-66vd2mdu	606	33	and	and	CC
cord-014597-66vd2mdu	606	34	product	product	NN
cord-014597-66vd2mdu	606	35	titer	titer	NN
cord-014597-66vd2mdu	606	36	achieved	achieve	VBN
cord-014597-66vd2mdu	606	37	for	for	IN
cord-014597-66vd2mdu	606	38	the	the	DT
cord-014597-66vd2mdu	606	39	different	different	JJ
cord-014597-66vd2mdu	606	40	culture	culture	NN
cord-014597-66vd2mdu	606	41	strategies	strategy	NNS
cord-014597-66vd2mdu	606	42	assessed	assess	VBD
cord-014597-66vd2mdu	606	43	(	(	-LRB-
cord-014597-66vd2mdu	606	44	batch	batch	NN
cord-014597-66vd2mdu	606	45	,	,	,
cord-014597-66vd2mdu	606	46	fortified	fortified	JJ
cord-014597-66vd2mdu	606	47	-	-	HYPH
cord-014597-66vd2mdu	606	48	batch	batch	NN
cord-014597-66vd2mdu	606	49	and	and	CC
cord-014597-66vd2mdu	606	50	fed	fed	JJ
cord-014597-66vd2mdu	606	51	-	-	HYPH
cord-014597-66vd2mdu	606	52	batch	batch	NN
cord-014597-66vd2mdu	606	53	)	)	-RRB-
cord-014597-66vd2mdu	606	54	are	be	VBP
cord-014597-66vd2mdu	606	55	presented	present	VBN
cord-014597-66vd2mdu	606	56	in	in	IN
cord-014597-66vd2mdu	606	57	table	table	NN
cord-014597-66vd2mdu	606	58	1	1	CD
cord-014597-66vd2mdu	606	59	.	.	.
cord-014597-66vd2mdu	607	1	In	in	IN
cord-014597-66vd2mdu	607	2	fortified	fortified	JJ
cord-014597-66vd2mdu	607	3	batch	batch	NN
cord-014597-66vd2mdu	607	4	an	an	DT
cord-014597-66vd2mdu	607	5	increase	increase	NN
cord-014597-66vd2mdu	607	6	in	in	IN
cord-014597-66vd2mdu	607	7	VCD	vcd	NN
cord-014597-66vd2mdu	607	8	of	of	IN
cord-014597-66vd2mdu	607	9	145	145	CD
cord-014597-66vd2mdu	607	10	%	%	NN
cord-014597-66vd2mdu	607	11	and	and	CC
cord-014597-66vd2mdu	607	12	also	also	RB
cord-014597-66vd2mdu	607	13	350	350	CD
cord-014597-66vd2mdu	607	14	%	%	NN
cord-014597-66vd2mdu	607	15	in	in	IN
cord-014597-66vd2mdu	607	16	product	product	NN
cord-014597-66vd2mdu	607	17	titer	titer	NN
cord-014597-66vd2mdu	607	18	were	be	VBD
cord-014597-66vd2mdu	607	19	obtained	obtain	VBN
cord-014597-66vd2mdu	607	20	compared	compare	VBN
cord-014597-66vd2mdu	607	21	with	with	IN
cord-014597-66vd2mdu	607	22	batch	batch	NN
cord-014597-66vd2mdu	607	23	.	.	.
cord-014597-66vd2mdu	608	1	In	in	IN
cord-014597-66vd2mdu	608	2	the	the	DT
cord-014597-66vd2mdu	608	3	fed	fed	JJ
cord-014597-66vd2mdu	608	4	-	-	HYPH
cord-014597-66vd2mdu	608	5	batch	batch	NN
cord-014597-66vd2mdu	608	6	culture	culture	NN
cord-014597-66vd2mdu	608	7	carried	carry	VBN
cord-014597-66vd2mdu	608	8	out	out	RP
cord-014597-66vd2mdu	608	9	(	(	-LRB-
cord-014597-66vd2mdu	608	10	Fig	Fig	NNP
cord-014597-66vd2mdu	608	11	.	.	.
cord-014597-66vd2mdu	608	12	1	1	CD
cord-014597-66vd2mdu	608	13	)	)	-RRB-
cord-014597-66vd2mdu	608	14	,	,	,
cord-014597-66vd2mdu	608	15	we	-PRON-	PRP
cord-014597-66vd2mdu	608	16	observed	observe	VBD
cord-014597-66vd2mdu	608	17	that	that	IN
cord-014597-66vd2mdu	608	18	alkali	alkali	JJ
cord-014597-66vd2mdu	608	19	buffer	buffer	NN
cord-014597-66vd2mdu	608	20	addition	addition	NN
cord-014597-66vd2mdu	608	21	profile	profile	NN
cord-014597-66vd2mdu	608	22	matched	match	VBD
cord-014597-66vd2mdu	608	23	the	the	DT
cord-014597-66vd2mdu	608	24	VCD	vcd	NN
cord-014597-66vd2mdu	608	25	evolution	evolution	NN
cord-014597-66vd2mdu	608	26	trend	trend	NN
cord-014597-66vd2mdu	608	27	.	.	.
cord-014597-66vd2mdu	609	1	Thus	thus	RB
cord-014597-66vd2mdu	609	2	,	,	,
cord-014597-66vd2mdu	609	3	the	the	DT
cord-014597-66vd2mdu	609	4	monitoring	monitoring	NN
cord-014597-66vd2mdu	609	5	of	of	IN
cord-014597-66vd2mdu	609	6	alkali	alkali	JJ
cord-014597-66vd2mdu	609	7	buffer	buffer	NN
cord-014597-66vd2mdu	609	8	addition	addition	NN
cord-014597-66vd2mdu	609	9	was	be	VBD
cord-014597-66vd2mdu	609	10	used	use	VBN
cord-014597-66vd2mdu	609	11	for	for	IN
cord-014597-66vd2mdu	609	12	estimating	estimate	VBG
cord-014597-66vd2mdu	609	13	the	the	DT
cord-014597-66vd2mdu	609	14	nutrients	nutrient	NNS
cord-014597-66vd2mdu	609	15	requirements	requirement	NNS
cord-014597-66vd2mdu	609	16	(	(	-LRB-
cord-014597-66vd2mdu	609	17	i.e.	i.e.	FW
cord-014597-66vd2mdu	609	18	the	the	DT
cord-014597-66vd2mdu	609	19	volume	volume	NN
cord-014597-66vd2mdu	609	20	of	of	IN
cord-014597-66vd2mdu	609	21	feeding	feed	VBG
cord-014597-66vd2mdu	609	22	medium	medium	NN
cord-014597-66vd2mdu	609	23	)	)	-RRB-
cord-014597-66vd2mdu	609	24	at	at	IN
cord-014597-66vd2mdu	609	25	any	any	DT
cord-014597-66vd2mdu	609	26	time	time	NN
cord-014597-66vd2mdu	609	27	during	during	IN
cord-014597-66vd2mdu	609	28	the	the	DT
cord-014597-66vd2mdu	609	29	fed	fed	NNP
cord-014597-66vd2mdu	609	30	batch	batch	NN
cord-014597-66vd2mdu	609	31	phase	phase	NN
cord-014597-66vd2mdu	609	32	.	.	.
cord-014597-66vd2mdu	610	1	The	the	DT
cord-014597-66vd2mdu	610	2	feeding	feeding	NN
cord-014597-66vd2mdu	610	3	strategy	strategy	NN
cord-014597-66vd2mdu	610	4	based	base	VBN
cord-014597-66vd2mdu	610	5	on	on	IN
cord-014597-66vd2mdu	610	6	alkali	alkali	JJ
cord-014597-66vd2mdu	610	7	buffer	buffer	NN
cord-014597-66vd2mdu	610	8	addition	addition	NN
cord-014597-66vd2mdu	610	9	enabled	enable	VBD
cord-014597-66vd2mdu	610	10	to	to	TO
cord-014597-66vd2mdu	610	11	maintain	maintain	VB
cord-014597-66vd2mdu	610	12	glucose	glucose	NN
cord-014597-66vd2mdu	610	13	concentration	concentration	NN
cord-014597-66vd2mdu	610	14	set	set	VBN
cord-014597-66vd2mdu	610	15	point	point	NN
cord-014597-66vd2mdu	610	16	therein	therein	IN
cord-014597-66vd2mdu	610	17	a	a	DT
cord-014597-66vd2mdu	610	18	narrow	narrow	JJ
cord-014597-66vd2mdu	610	19	range	range	NN
cord-014597-66vd2mdu	610	20	during	during	IN
cord-014597-66vd2mdu	610	21	fed	fed	JJ
cord-014597-66vd2mdu	610	22	-	-	HYPH
cord-014597-66vd2mdu	610	23	batch	batch	NN
cord-014597-66vd2mdu	610	24	phase	phase	NN
cord-014597-66vd2mdu	610	25	(	(	-LRB-
cord-014597-66vd2mdu	610	26	around	around	RB
cord-014597-66vd2mdu	610	27	20	20	CD
cord-014597-66vd2mdu	610	28	mM	mm	CD
cord-014597-66vd2mdu	610	29	)	)	-RRB-
cord-014597-66vd2mdu	610	30	.	.	.
cord-014597-66vd2mdu	611	1	As	as	IN
cord-014597-66vd2mdu	611	2	a	a	DT
cord-014597-66vd2mdu	611	3	result	result	NN
cord-014597-66vd2mdu	611	4	,	,	,
cord-014597-66vd2mdu	611	5	higher	high	JJR
cord-014597-66vd2mdu	611	6	VCD	vcd	NN
cord-014597-66vd2mdu	611	7	(	(	-LRB-
cord-014597-66vd2mdu	611	8	16.6·10	16.6·10	CD
cord-014597-66vd2mdu	611	9	6	6	CD
cord-014597-66vd2mdu	611	10	cells	cell	NNS
cord-014597-66vd2mdu	611	11	/	/	SYM
cord-014597-66vd2mdu	611	12	mL	ml	NN
cord-014597-66vd2mdu	611	13	)	)	-RRB-
cord-014597-66vd2mdu	611	14	was	be	VBD
cord-014597-66vd2mdu	611	15	obtained	obtain	VBN
cord-014597-66vd2mdu	611	16	when	when	WRB
cord-014597-66vd2mdu	611	17	compared	compare	VBN
cord-014597-66vd2mdu	611	18	with	with	IN
cord-014597-66vd2mdu	611	19	both	both	DT
cord-014597-66vd2mdu	611	20	batch	batch	JJ
cord-014597-66vd2mdu	611	21	references	reference	NNS
cord-014597-66vd2mdu	611	22	:	:	:
cord-014597-66vd2mdu	611	23	VCD	VCD	NNP
cord-014597-66vd2mdu	611	24	was	be	VBD
cord-014597-66vd2mdu	611	25	enhanced	enhance	VBN
cord-014597-66vd2mdu	611	26	to	to	IN
cord-014597-66vd2mdu	611	27	241	241	CD
cord-014597-66vd2mdu	611	28	%	%	NN
cord-014597-66vd2mdu	611	29	and	and	CC
cord-014597-66vd2mdu	611	30	39	39	CD
cord-014597-66vd2mdu	611	31	%	%	NN
cord-014597-66vd2mdu	611	32	and	and	CC
cord-014597-66vd2mdu	611	33	an	an	DT
cord-014597-66vd2mdu	611	34	increase	increase	NN
cord-014597-66vd2mdu	611	35	up	up	RB
cord-014597-66vd2mdu	611	36	to	to	TO
cord-014597-66vd2mdu	611	37	381	381	CD
cord-014597-66vd2mdu	611	38	%	%	NN
cord-014597-66vd2mdu	611	39	and	and	CC
cord-014597-66vd2mdu	611	40	7	7	CD
cord-014597-66vd2mdu	611	41	%	%	NN
cord-014597-66vd2mdu	611	42	in	in	IN
cord-014597-66vd2mdu	611	43	product	product	NN
cord-014597-66vd2mdu	611	44	titer	titer	NN
cord-014597-66vd2mdu	611	45	in	in	IN
cord-014597-66vd2mdu	611	46	respect	respect	NN
cord-014597-66vd2mdu	611	47	to	to	TO
cord-014597-66vd2mdu	611	48	batch	batch	VB
cord-014597-66vd2mdu	611	49	and	and	CC
cord-014597-66vd2mdu	611	50	fortified	fortified	JJ
cord-014597-66vd2mdu	611	51	batch	batch	NN
cord-014597-66vd2mdu	611	52	respectively	respectively	RB
cord-014597-66vd2mdu	611	53	.	.	.
cord-014597-66vd2mdu	612	1	The	the	DT
cord-014597-66vd2mdu	612	2	results	result	NNS
cord-014597-66vd2mdu	612	3	prove	prove	VBP
cord-014597-66vd2mdu	612	4	that	that	IN
cord-014597-66vd2mdu	612	5	fed	feed	VBN
cord-014597-66vd2mdu	612	6	-	-	HYPH
cord-014597-66vd2mdu	612	7	batch	batch	NN
cord-014597-66vd2mdu	612	8	strategy	strategy	NN
cord-014597-66vd2mdu	612	9	based	base	VBN
cord-014597-66vd2mdu	612	10	on	on	IN
cord-014597-66vd2mdu	612	11	the	the	DT
cord-014597-66vd2mdu	612	12	alkali	alkali	JJ
cord-014597-66vd2mdu	612	13	buffer	buffer	NN
cord-014597-66vd2mdu	612	14	addition	addition	NN
cord-014597-66vd2mdu	612	15	is	be	VBZ
cord-014597-66vd2mdu	612	16	a	a	DT
cord-014597-66vd2mdu	612	17	robust	robust	JJ
cord-014597-66vd2mdu	612	18	on	on	IN
cord-014597-66vd2mdu	612	19	-	-	HYPH
cord-014597-66vd2mdu	612	20	line	line	NN
cord-014597-66vd2mdu	612	21	monitoring	monitoring	NN
cord-014597-66vd2mdu	612	22	method	method	NN
cord-014597-66vd2mdu	612	23	that	that	WDT
cord-014597-66vd2mdu	612	24	enables	enable	VBZ
cord-014597-66vd2mdu	612	25	to	to	TO
cord-014597-66vd2mdu	612	26	optimize	optimize	VB
cord-014597-66vd2mdu	612	27	the	the	DT
cord-014597-66vd2mdu	612	28	feeding	feeding	NN
cord-014597-66vd2mdu	612	29	strategy	strategy	NN
cord-014597-66vd2mdu	612	30	in	in	IN
cord-014597-66vd2mdu	612	31	a	a	DT
cord-014597-66vd2mdu	612	32	fed	feed	VBN
cord-014597-66vd2mdu	612	33	-	-	HYPH
cord-014597-66vd2mdu	612	34	batch	batch	NN
cord-014597-66vd2mdu	612	35	cultures	culture	NNS
cord-014597-66vd2mdu	612	36	.	.	.
cord-014597-66vd2mdu	613	1	Three	three	CD
cord-014597-66vd2mdu	613	2	different	different	JJ
cord-014597-66vd2mdu	613	3	culture	culture	NN
cord-014597-66vd2mdu	613	4	strategies	strategy	NNS
cord-014597-66vd2mdu	613	5	have	have	VBP
cord-014597-66vd2mdu	613	6	been	be	VBN
cord-014597-66vd2mdu	613	7	tested	test	VBN
cord-014597-66vd2mdu	613	8	in	in	IN
cord-014597-66vd2mdu	613	9	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	613	10	with	with	IN
cord-014597-66vd2mdu	613	11	a	a	DT
cord-014597-66vd2mdu	613	12	HEK293	HEK293	NNP
cord-014597-66vd2mdu	613	13	cell	cell	NN
cord-014597-66vd2mdu	613	14	line	line	NN
cord-014597-66vd2mdu	613	15	producing	produce	VBG
cord-014597-66vd2mdu	613	16	IFN	IFN	NNP
cord-014597-66vd2mdu	613	17	-	-	HYPH
cord-014597-66vd2mdu	613	18	γ	γ	NNP
cord-014597-66vd2mdu	613	19	.	.	.
cord-014597-66vd2mdu	614	1	Results	result	NNS
cord-014597-66vd2mdu	614	2	show	show	VBP
cord-014597-66vd2mdu	614	3	as	as	IN
cord-014597-66vd2mdu	614	4	the	the	DT
cord-014597-66vd2mdu	614	5	higher	high	JJR
cord-014597-66vd2mdu	614	6	VCD	vcd	NN
cord-014597-66vd2mdu	614	7	is	be	VBZ
cord-014597-66vd2mdu	614	8	reached	reach	VBN
cord-014597-66vd2mdu	614	9	,	,	,
cord-014597-66vd2mdu	614	10	the	the	DT
cord-014597-66vd2mdu	614	11	higher	high	JJR
cord-014597-66vd2mdu	614	12	product	product	NN
cord-014597-66vd2mdu	614	13	concentration	concentration	NN
cord-014597-66vd2mdu	614	14	is	be	VBZ
cord-014597-66vd2mdu	614	15	achieved	achieve	VBN
cord-014597-66vd2mdu	614	16	.	.	.
cord-014597-66vd2mdu	615	1	Therefore	therefore	RB
cord-014597-66vd2mdu	615	2	,	,	,
cord-014597-66vd2mdu	615	3	from	from	IN
cord-014597-66vd2mdu	615	4	bioprocess	bioprocess	NNP
cord-014597-66vd2mdu	615	5	development	development	NNP
cord-014597-66vd2mdu	615	6	point	point	NN
cord-014597-66vd2mdu	615	7	of	of	IN
cord-014597-66vd2mdu	615	8	view	view	NN
cord-014597-66vd2mdu	615	9	,	,	,
cord-014597-66vd2mdu	615	10	it	-PRON-	PRP
cord-014597-66vd2mdu	615	11	is	be	VBZ
cord-014597-66vd2mdu	615	12	very	very	RB
cord-014597-66vd2mdu	615	13	interesting	interesting	JJ
cord-014597-66vd2mdu	615	14	to	to	TO
cord-014597-66vd2mdu	615	15	implement	implement	VB
cord-014597-66vd2mdu	615	16	strategies	strategy	NNS
cord-014597-66vd2mdu	615	17	with	with	IN
cord-014597-66vd2mdu	615	18	higher	high	JJR
cord-014597-66vd2mdu	615	19	VCD	vcd	NN
cord-014597-66vd2mdu	615	20	outcome	outcome	NN
cord-014597-66vd2mdu	615	21	,	,	,
cord-014597-66vd2mdu	615	22	such	such	JJ
cord-014597-66vd2mdu	615	23	as	as	IN
cord-014597-66vd2mdu	615	24	fed	fed	JJ
cord-014597-66vd2mdu	615	25	-	-	HYPH
cord-014597-66vd2mdu	615	26	batch	batch	NN
cord-014597-66vd2mdu	615	27	operation	operation	NN
cord-014597-66vd2mdu	615	28	mode	mode	NN
cord-014597-66vd2mdu	615	29	.	.	.
cord-014597-66vd2mdu	616	1	In	in	IN
cord-014597-66vd2mdu	616	2	this	this	DT
cord-014597-66vd2mdu	616	3	context	context	NN
cord-014597-66vd2mdu	616	4	,	,	,
cord-014597-66vd2mdu	616	5	a	a	DT
cord-014597-66vd2mdu	616	6	new	new	JJ
cord-014597-66vd2mdu	616	7	robust	robust	JJ
cord-014597-66vd2mdu	616	8	method	method	NN
cord-014597-66vd2mdu	616	9	for	for	IN
cord-014597-66vd2mdu	616	10	VCD	vcd	NN
cord-014597-66vd2mdu	616	11	estimation	estimation	NN
cord-014597-66vd2mdu	616	12	in	in	IN
cord-014597-66vd2mdu	616	13	fed	fed	NNP
cord-014597-66vd2mdu	616	14	-	-	HYPH
cord-014597-66vd2mdu	616	15	batch	batch	NNP
cord-014597-66vd2mdu	616	16	was	be	VBD
cord-014597-66vd2mdu	616	17	applied	apply	VBN
cord-014597-66vd2mdu	616	18	.	.	.
cord-014597-66vd2mdu	617	1	The	the	DT
cord-014597-66vd2mdu	617	2	alkali	alkali	JJ
cord-014597-66vd2mdu	617	3	buffer	buffer	NN
cord-014597-66vd2mdu	617	4	addition	addition	NN
cord-014597-66vd2mdu	617	5	necessary	necessary	JJ
cord-014597-66vd2mdu	617	6	for	for	IN
cord-014597-66vd2mdu	617	7	maintaining	maintain	VBG
cord-014597-66vd2mdu	617	8	the	the	DT
cord-014597-66vd2mdu	617	9	pH	pH	NNP
cord-014597-66vd2mdu	617	10	set	set	NN
cord-014597-66vd2mdu	617	11	-	-	HYPH
cord-014597-66vd2mdu	617	12	point	point	NN
cord-014597-66vd2mdu	617	13	is	be	VBZ
cord-014597-66vd2mdu	617	14	an	an	DT
cord-014597-66vd2mdu	617	15	on	on	IN
cord-014597-66vd2mdu	617	16	-	-	HYPH
cord-014597-66vd2mdu	617	17	line	line	NN
cord-014597-66vd2mdu	617	18	reliable	reliable	JJ
cord-014597-66vd2mdu	617	19	and	and	CC
cord-014597-66vd2mdu	617	20	easy	easy	JJ
cord-014597-66vd2mdu	617	21	measuring	measuring	NN
cord-014597-66vd2mdu	617	22	variable	variable	NN
cord-014597-66vd2mdu	617	23	that	that	WDT
cord-014597-66vd2mdu	617	24	provides	provide	VBZ
cord-014597-66vd2mdu	617	25	information	information	NN
cord-014597-66vd2mdu	617	26	about	about	IN
cord-014597-66vd2mdu	617	27	by	by	IN
cord-014597-66vd2mdu	617	28	-	-	HYPH
cord-014597-66vd2mdu	617	29	products	product	NNS
cord-014597-66vd2mdu	617	30	formation	formation	NN
cord-014597-66vd2mdu	617	31	(	(	-LRB-
cord-014597-66vd2mdu	617	32	mainly	mainly	RB
cord-014597-66vd2mdu	617	33	lactic	lactic	JJ
cord-014597-66vd2mdu	617	34	acid	acid	NN
cord-014597-66vd2mdu	617	35	)	)	-RRB-
cord-014597-66vd2mdu	617	36	.	.	.
cord-014597-66vd2mdu	618	1	The	the	DT
cord-014597-66vd2mdu	618	2	monitoring	monitoring	NN
cord-014597-66vd2mdu	618	3	of	of	IN
cord-014597-66vd2mdu	618	4	this	this	DT
cord-014597-66vd2mdu	618	5	variable	variable	NN
cord-014597-66vd2mdu	618	6	can	can	MD
cord-014597-66vd2mdu	618	7	provide	provide	VB
cord-014597-66vd2mdu	618	8	information	information	NN
cord-014597-66vd2mdu	618	9	about	about	IN
cord-014597-66vd2mdu	618	10	the	the	DT
cord-014597-66vd2mdu	618	11	cell	cell	NN
cord-014597-66vd2mdu	618	12	concentration	concentration	NN
cord-014597-66vd2mdu	618	13	,	,	,
cord-014597-66vd2mdu	618	14	activity	activity	NN
cord-014597-66vd2mdu	618	15	and	and	CC
cord-014597-66vd2mdu	618	16	metabolism	metabolism	NN
cord-014597-66vd2mdu	618	17	,	,	,
cord-014597-66vd2mdu	618	18	to	to	TO
cord-014597-66vd2mdu	618	19	detect	detect	VB
cord-014597-66vd2mdu	618	20	changes	change	NNS
cord-014597-66vd2mdu	618	21	in	in	IN
cord-014597-66vd2mdu	618	22	culture	culture	NN
cord-014597-66vd2mdu	618	23	.	.	.
cord-014597-66vd2mdu	619	1	Besides	besides	IN
cord-014597-66vd2mdu	619	2	that	that	DT
cord-014597-66vd2mdu	619	3	,	,	,
cord-014597-66vd2mdu	619	4	a	a	DT
cord-014597-66vd2mdu	619	5	relationship	relationship	NN
cord-014597-66vd2mdu	619	6	between	between	IN
cord-014597-66vd2mdu	619	7	alkali	alkali	NN
cord-014597-66vd2mdu	619	8	buffer	buffer	NN
cord-014597-66vd2mdu	619	9	addition	addition	NN
cord-014597-66vd2mdu	619	10	and	and	CC
cord-014597-66vd2mdu	619	11	VCD	vcd	NN
cord-014597-66vd2mdu	619	12	can	can	MD
cord-014597-66vd2mdu	619	13	be	be	VB
cord-014597-66vd2mdu	619	14	established	establish	VBN
cord-014597-66vd2mdu	619	15	since	since	IN
cord-014597-66vd2mdu	619	16	the	the	DT
cord-014597-66vd2mdu	619	17	first	first	JJ
cord-014597-66vd2mdu	619	18	is	be	VBZ
cord-014597-66vd2mdu	619	19	strongly	strongly	RB
cord-014597-66vd2mdu	619	20	correlated	correlate	VBN
cord-014597-66vd2mdu	619	21	with	with	IN
cord-014597-66vd2mdu	619	22	cell	cell	NN
cord-014597-66vd2mdu	619	23	growth	growth	NN
cord-014597-66vd2mdu	619	24	and	and	CC
cord-014597-66vd2mdu	619	25	metabolites	metabolite	NNS
cord-014597-66vd2mdu	619	26	consumption	consumption	NN
cord-014597-66vd2mdu	619	27	/	/	SYM
cord-014597-66vd2mdu	619	28	formation	formation	NN
cord-014597-66vd2mdu	619	29	.	.	.
cord-014597-66vd2mdu	620	1	The	the	DT
cord-014597-66vd2mdu	620	2	application	application	NN
cord-014597-66vd2mdu	620	3	of	of	IN
cord-014597-66vd2mdu	620	4	alkali	alkali	JJ
cord-014597-66vd2mdu	620	5	buffer	buffer	NN
cord-014597-66vd2mdu	620	6	addition	addition	NN
cord-014597-66vd2mdu	620	7	measure	measure	NN
cord-014597-66vd2mdu	620	8	to	to	TO
cord-014597-66vd2mdu	620	9	implement	implement	VB
cord-014597-66vd2mdu	620	10	an	an	DT
cord-014597-66vd2mdu	620	11	optimal	optimal	JJ
cord-014597-66vd2mdu	620	12	feeding	feeding	NN
cord-014597-66vd2mdu	620	13	strategy	strategy	NN
cord-014597-66vd2mdu	620	14	in	in	IN
cord-014597-66vd2mdu	620	15	fed	fed	NNP
cord-014597-66vd2mdu	620	16	-	-	HYPH
cord-014597-66vd2mdu	620	17	batch	batch	NN
cord-014597-66vd2mdu	620	18	permits	permit	NNS
cord-014597-66vd2mdu	620	19	to	to	TO
cord-014597-66vd2mdu	620	20	enhance	enhance	VB
cord-014597-66vd2mdu	620	21	VCD	vcd	NN
cord-014597-66vd2mdu	620	22	and	and	CC
cord-014597-66vd2mdu	620	23	product	product	NN
cord-014597-66vd2mdu	620	24	titer	titer	NN
cord-014597-66vd2mdu	620	25	when	when	WRB
cord-014597-66vd2mdu	620	26	comparing	compare	VBG
cord-014597-66vd2mdu	620	27	with	with	IN
cord-014597-66vd2mdu	620	28	batch	batch	NN
cord-014597-66vd2mdu	620	29	strategies	strategy	NNS
cord-014597-66vd2mdu	620	30	.	.	.
cord-014597-66vd2mdu	621	1	A	a	DT
cord-014597-66vd2mdu	621	2	novel	novel	JJ
cord-014597-66vd2mdu	621	3	approach	approach	NN
cord-014597-66vd2mdu	621	4	to	to	IN
cord-014597-66vd2mdu	621	5	high	high	JJ
cord-014597-66vd2mdu	621	6	throughput	throughput	NN
cord-014597-66vd2mdu	621	7	screening	screening	NN
cord-014597-66vd2mdu	621	8	for	for	IN
cord-014597-66vd2mdu	621	9	perfusion	perfusion	NN
cord-014597-66vd2mdu	621	10	Background	Background	NNP
cord-014597-66vd2mdu	621	11	Perfusion	perfusion	NN
cord-014597-66vd2mdu	621	12	systems	system	NNS
cord-014597-66vd2mdu	621	13	for	for	IN
cord-014597-66vd2mdu	621	14	suspended	suspend	VBN
cord-014597-66vd2mdu	621	15	mammalian	mammalian	JJ
cord-014597-66vd2mdu	621	16	cells	cell	NNS
cord-014597-66vd2mdu	621	17	raise	raise	VBP
cord-014597-66vd2mdu	621	18	growing	grow	VBG
cord-014597-66vd2mdu	621	19	interest	interest	NN
cord-014597-66vd2mdu	621	20	in	in	IN
cord-014597-66vd2mdu	621	21	the	the	DT
cord-014597-66vd2mdu	621	22	biomanufacturing	biomanufacturing	NN
cord-014597-66vd2mdu	621	23	industry	industry	NN
cord-014597-66vd2mdu	621	24	.	.	.
cord-014597-66vd2mdu	622	1	Continuous	continuous	JJ
cord-014597-66vd2mdu	622	2	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	622	3	is	be	VBZ
cord-014597-66vd2mdu	622	4	growing	grow	VBG
cord-014597-66vd2mdu	622	5	in	in	IN
cord-014597-66vd2mdu	622	6	the	the	DT
cord-014597-66vd2mdu	622	7	field	field	NN
cord-014597-66vd2mdu	622	8	and	and	CC
cord-014597-66vd2mdu	622	9	is	be	VBZ
cord-014597-66vd2mdu	622	10	encouraged	encourage	VBN
cord-014597-66vd2mdu	622	11	by	by	IN
cord-014597-66vd2mdu	622	12	health	health	NN
cord-014597-66vd2mdu	622	13	authorities	authority	NNS
cord-014597-66vd2mdu	622	14	[	[	-LRB-
cord-014597-66vd2mdu	622	15	1	1	CD
cord-014597-66vd2mdu	622	16	,	,	,
cord-014597-66vd2mdu	622	17	2	2	CD
cord-014597-66vd2mdu	622	18	,	,	,
cord-014597-66vd2mdu	622	19	3	3	CD
cord-014597-66vd2mdu	622	20	]	]	-RRB-
cord-014597-66vd2mdu	622	21	.	.	.
cord-014597-66vd2mdu	623	1	This	this	DT
cord-014597-66vd2mdu	623	2	work	work	NN
cord-014597-66vd2mdu	623	3	addresses	address	VBZ
cord-014597-66vd2mdu	623	4	scale	scale	VBP
cord-014597-66vd2mdu	623	5	down	down	RP
cord-014597-66vd2mdu	623	6	limitations	limitation	NNS
cord-014597-66vd2mdu	623	7	inherent	inherent	JJ
cord-014597-66vd2mdu	623	8	to	to	IN
cord-014597-66vd2mdu	623	9	continuous	continuous	JJ
cord-014597-66vd2mdu	623	10	media	medium	NNS
cord-014597-66vd2mdu	623	11	exchange	exchange	NN
cord-014597-66vd2mdu	623	12	and	and	CC
cord-014597-66vd2mdu	623	13	cell	cell	NN
cord-014597-66vd2mdu	623	14	retention	retention	NN
cord-014597-66vd2mdu	623	15	by	by	IN
cord-014597-66vd2mdu	623	16	using	use	VBG
cord-014597-66vd2mdu	623	17	a	a	DT
cord-014597-66vd2mdu	623	18	semi	semi	JJ
cord-014597-66vd2mdu	623	19	-	-	JJ
cord-014597-66vd2mdu	623	20	continuous	continuous	JJ
cord-014597-66vd2mdu	623	21	system	system	NN
cord-014597-66vd2mdu	623	22	.	.	.
cord-014597-66vd2mdu	624	1	Data	datum	NNS
cord-014597-66vd2mdu	624	2	was	be	VBD
cord-014597-66vd2mdu	624	3	generated	generate	VBN
cord-014597-66vd2mdu	624	4	with	with	IN
cord-014597-66vd2mdu	624	5	a	a	DT
cord-014597-66vd2mdu	624	6	set	set	NN
cord-014597-66vd2mdu	624	7	of	of	IN
cord-014597-66vd2mdu	624	8	different	different	JJ
cord-014597-66vd2mdu	624	9	clones	clone	NNS
cord-014597-66vd2mdu	624	10	that	that	WDT
cord-014597-66vd2mdu	624	11	were	be	VBD
cord-014597-66vd2mdu	624	12	previously	previously	RB
cord-014597-66vd2mdu	624	13	studied	study	VBN
cord-014597-66vd2mdu	624	14	in	in	IN
cord-014597-66vd2mdu	624	15	fed	fed	JJ
cord-014597-66vd2mdu	624	16	-	-	HYPH
cord-014597-66vd2mdu	624	17	batch	batch	NN
cord-014597-66vd2mdu	624	18	mode	mode	NN
cord-014597-66vd2mdu	624	19	[	[	-LRB-
cord-014597-66vd2mdu	624	20	4	4	CD
cord-014597-66vd2mdu	624	21	]	]	-RRB-
cord-014597-66vd2mdu	624	22	.	.	.
cord-014597-66vd2mdu	625	1	Materials	material	NNS
cord-014597-66vd2mdu	625	2	and	and	CC
cord-014597-66vd2mdu	625	3	methods	method	NNS
cord-014597-66vd2mdu	625	4	4	4	CD
cord-014597-66vd2mdu	625	5	CHO	CHO	NNP
cord-014597-66vd2mdu	625	6	-	-	HYPH
cord-014597-66vd2mdu	625	7	K1	K1	NNP
cord-014597-66vd2mdu	625	8	cell	cell	NN
cord-014597-66vd2mdu	625	9	lines	line	NNS
cord-014597-66vd2mdu	625	10	expressing	express	VBG
cord-014597-66vd2mdu	625	11	the	the	DT
cord-014597-66vd2mdu	625	12	same	same	JJ
cord-014597-66vd2mdu	625	13	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	625	14	antibody	antibody	NN
cord-014597-66vd2mdu	625	15	(	(	-LRB-
cord-014597-66vd2mdu	625	16	mAb	mAb	NNS
cord-014597-66vd2mdu	625	17	)	)	-RRB-
cord-014597-66vd2mdu	625	18	and	and	CC
cord-014597-66vd2mdu	625	19	issued	issue	VBN
cord-014597-66vd2mdu	625	20	from	from	IN
cord-014597-66vd2mdu	625	21	the	the	DT
cord-014597-66vd2mdu	625	22	same	same	JJ
cord-014597-66vd2mdu	625	23	transfection	transfection	NN
cord-014597-66vd2mdu	625	24	were	be	VBD
cord-014597-66vd2mdu	625	25	used	use	VBN
cord-014597-66vd2mdu	625	26	as	as	IN
cord-014597-66vd2mdu	625	27	models	model	NNS
cord-014597-66vd2mdu	625	28	.	.	.
cord-014597-66vd2mdu	626	1	3.5L	3.5L	NNS
cord-014597-66vd2mdu	626	2	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	626	3	(	(	-LRB-
cord-014597-66vd2mdu	626	4	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	626	5	)	)	-RRB-
cord-014597-66vd2mdu	626	6	were	be	VBD
cord-014597-66vd2mdu	626	7	used	use	VBN
cord-014597-66vd2mdu	626	8	for	for	IN
cord-014597-66vd2mdu	626	9	fed	fed	NN
cord-014597-66vd2mdu	626	10	-	-	HYPH
cord-014597-66vd2mdu	626	11	batch	batch	NN
cord-014597-66vd2mdu	626	12	and	and	CC
cord-014597-66vd2mdu	626	13	perfusion	perfusion	NN
cord-014597-66vd2mdu	626	14	production	production	NN
cord-014597-66vd2mdu	626	15	runs	run	NNS
cord-014597-66vd2mdu	626	16	.	.	.
cord-014597-66vd2mdu	627	1	The	the	DT
cord-014597-66vd2mdu	627	2	perfusion	perfusion	NN
cord-014597-66vd2mdu	627	3	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	627	4	were	be	VBD
cord-014597-66vd2mdu	627	5	run	run	VBN
cord-014597-66vd2mdu	627	6	using	use	VBG
cord-014597-66vd2mdu	627	7	an	an	DT
cord-014597-66vd2mdu	627	8	alternating	alternate	VBG
cord-014597-66vd2mdu	627	9	tangential	tangential	JJ
cord-014597-66vd2mdu	627	10	flow	flow	NN
cord-014597-66vd2mdu	627	11	filtration	filtration	NN
cord-014597-66vd2mdu	627	12	device	device	NN
cord-014597-66vd2mdu	627	13	(	(	-LRB-
cord-014597-66vd2mdu	627	14	Repligen	Repligen	NNP
cord-014597-66vd2mdu	627	15	,	,	,
cord-014597-66vd2mdu	627	16	XCell	XCell	NNP
cord-014597-66vd2mdu	627	17	™	™	NN
cord-014597-66vd2mdu	627	18	ATF	ATF	NNP
cord-014597-66vd2mdu	627	19	2	2	CD
cord-014597-66vd2mdu	627	20	System	System	NNP
cord-014597-66vd2mdu	627	21	)	)	-RRB-
cord-014597-66vd2mdu	627	22	.	.	.
cord-014597-66vd2mdu	628	1	The	the	DT
cord-014597-66vd2mdu	628	2	cell	cell	NN
cord-014597-66vd2mdu	628	3	biomass	biomass	NN
cord-014597-66vd2mdu	628	4	was	be	VBD
cord-014597-66vd2mdu	628	5	controlled	control	VBN
cord-014597-66vd2mdu	628	6	by	by	IN
cord-014597-66vd2mdu	628	7	removing	remove	VBG
cord-014597-66vd2mdu	628	8	cells	cell	NNS
cord-014597-66vd2mdu	628	9	through	through	IN
cord-014597-66vd2mdu	628	10	a	a	DT
cord-014597-66vd2mdu	628	11	bleed	bleed	NN
cord-014597-66vd2mdu	628	12	line	line	NN
cord-014597-66vd2mdu	628	13	and	and	CC
cord-014597-66vd2mdu	628	14	was	be	VBD
cord-014597-66vd2mdu	628	15	controlled	control	VBN
cord-014597-66vd2mdu	628	16	using	use	VBG
cord-014597-66vd2mdu	628	17	a	a	DT
cord-014597-66vd2mdu	628	18	biocapacitance	biocapacitance	NN
cord-014597-66vd2mdu	628	19	probe	probe	NN
cord-014597-66vd2mdu	628	20	(	(	-LRB-
cord-014597-66vd2mdu	628	21	Hamilton	Hamilton	NNP
cord-014597-66vd2mdu	628	22	,	,	,
cord-014597-66vd2mdu	628	23	Incyte	Incyte	NNP
cord-014597-66vd2mdu	628	24	)	)	-RRB-
cord-014597-66vd2mdu	628	25	.	.	.
cord-014597-66vd2mdu	629	1	The	the	DT
cord-014597-66vd2mdu	629	2	perfusion	perfusion	NN
cord-014597-66vd2mdu	629	3	rate	rate	NN
cord-014597-66vd2mdu	629	4	(	(	-LRB-
cord-014597-66vd2mdu	629	5	D	d	NN
cord-014597-66vd2mdu	629	6	)	)	-RRB-
cord-014597-66vd2mdu	629	7	was	be	VBD
cord-014597-66vd2mdu	629	8	fixed	fix	VBN
cord-014597-66vd2mdu	629	9	to	to	IN
cord-014597-66vd2mdu	629	10	one	one	CD
cord-014597-66vd2mdu	629	11	vessel	vessel	NN
cord-014597-66vd2mdu	629	12	volume	volume	NN
cord-014597-66vd2mdu	629	13	a	a	DT
cord-014597-66vd2mdu	629	14	day	day	NN
cord-014597-66vd2mdu	629	15	(	(	-LRB-
cord-014597-66vd2mdu	629	16	vvd	vvd	NNP
cord-014597-66vd2mdu	629	17	-1	-1	NNP
cord-014597-66vd2mdu	629	18	)	)	-RRB-
cord-014597-66vd2mdu	629	19	.	.	.
cord-014597-66vd2mdu	630	1	The	the	DT
cord-014597-66vd2mdu	630	2	semi	semi	JJ
cord-014597-66vd2mdu	630	3	-	-	JJ
cord-014597-66vd2mdu	630	4	continuous	continuous	JJ
cord-014597-66vd2mdu	630	5	runs	run	NNS
cord-014597-66vd2mdu	630	6	were	be	VBD
cord-014597-66vd2mdu	630	7	made	make	VBN
cord-014597-66vd2mdu	630	8	in	in	IN
cord-014597-66vd2mdu	630	9	50	50	CD
cord-014597-66vd2mdu	630	10	mL	ml	NN
cord-014597-66vd2mdu	630	11	shake	shake	NN
cord-014597-66vd2mdu	630	12	tube	tube	NN
cord-014597-66vd2mdu	630	13	(	(	-LRB-
cord-014597-66vd2mdu	630	14	TPP	TPP	NNP
cord-014597-66vd2mdu	630	15	,	,	,
cord-014597-66vd2mdu	630	16	TubeSpin	TubeSpin	NNP
cord-014597-66vd2mdu	630	17	®	®	,
cord-014597-66vd2mdu	630	18	Bioreactor	Bioreactor	NNP
cord-014597-66vd2mdu	630	19	50	50	CD
cord-014597-66vd2mdu	630	20	)	)	-RRB-
cord-014597-66vd2mdu	630	21	.	.	.
cord-014597-66vd2mdu	631	1	Once	once	RB
cord-014597-66vd2mdu	631	2	a	a	DT
cord-014597-66vd2mdu	631	3	day	day	NN
cord-014597-66vd2mdu	631	4	,	,	,
cord-014597-66vd2mdu	631	5	the	the	DT
cord-014597-66vd2mdu	631	6	tubes	tube	NNS
cord-014597-66vd2mdu	631	7	were	be	VBD
cord-014597-66vd2mdu	631	8	centrifuged	centrifuge	VBN
cord-014597-66vd2mdu	631	9	(	(	-LRB-
cord-014597-66vd2mdu	631	10	5	5	CD
cord-014597-66vd2mdu	631	11	min	min	NN
cord-014597-66vd2mdu	631	12	,	,	,
cord-014597-66vd2mdu	631	13	200	200	CD
cord-014597-66vd2mdu	631	14	g	g	NN
cord-014597-66vd2mdu	631	15	)	)	-RRB-
cord-014597-66vd2mdu	631	16	,	,	,
cord-014597-66vd2mdu	631	17	the	the	DT
cord-014597-66vd2mdu	631	18	supernatant	supernatant	NN
cord-014597-66vd2mdu	631	19	removed	remove	VBN
cord-014597-66vd2mdu	631	20	(	(	-LRB-
cord-014597-66vd2mdu	631	21	to	to	TO
cord-014597-66vd2mdu	631	22	mimic	mimic	VB
cord-014597-66vd2mdu	631	23	a	a	DT
cord-014597-66vd2mdu	631	24	perfusion	perfusion	NN
cord-014597-66vd2mdu	631	25	rate	rate	NN
cord-014597-66vd2mdu	631	26	of	of	IN
cord-014597-66vd2mdu	631	27	1	1	CD
cord-014597-66vd2mdu	631	28	vvd	vvd	NNP
cord-014597-66vd2mdu	631	29	-1	-1	NNP
cord-014597-66vd2mdu	631	30	)	)	-RRB-
cord-014597-66vd2mdu	631	31	,	,	,
cord-014597-66vd2mdu	631	32	replaced	replace	VBN
cord-014597-66vd2mdu	631	33	with	with	IN
cord-014597-66vd2mdu	631	34	fresh	fresh	JJ
cord-014597-66vd2mdu	631	35	media	medium	NNS
cord-014597-66vd2mdu	631	36	and	and	CC
cord-014597-66vd2mdu	631	37	cells	cell	NNS
cord-014597-66vd2mdu	631	38	were	be	VBD
cord-014597-66vd2mdu	631	39	re	re	VBN
cord-014597-66vd2mdu	631	40	-	-	VBN
cord-014597-66vd2mdu	631	41	suspended	suspend	VBN
cord-014597-66vd2mdu	631	42	.	.	.
cord-014597-66vd2mdu	632	1	The	the	DT
cord-014597-66vd2mdu	632	2	clone	clone	NN
cord-014597-66vd2mdu	632	3	's	's	POS
cord-014597-66vd2mdu	632	4	growth	growth	NN
cord-014597-66vd2mdu	632	5	potential	potential	NN
cord-014597-66vd2mdu	632	6	were	be	VBD
cord-014597-66vd2mdu	632	7	preserved	preserve	VBN
cord-014597-66vd2mdu	632	8	across	across	IN
cord-014597-66vd2mdu	632	9	the	the	DT
cord-014597-66vd2mdu	632	10	systems	system	NNS
cord-014597-66vd2mdu	632	11	(	(	-LRB-
cord-014597-66vd2mdu	632	12	Fig	Fig	NNP
cord-014597-66vd2mdu	632	13	.	.	NNP
cord-014597-66vd2mdu	632	14	1	1	CD
cord-014597-66vd2mdu	632	15	)	)	-RRB-
cord-014597-66vd2mdu	632	16	.	.	.
cord-014597-66vd2mdu	633	1	Clone	clone	NN
cord-014597-66vd2mdu	633	2	#	#	NN
cord-014597-66vd2mdu	633	3	3	3	CD
cord-014597-66vd2mdu	633	4	always	always	RB
cord-014597-66vd2mdu	633	5	reached	reach	VBD
cord-014597-66vd2mdu	633	6	the	the	DT
cord-014597-66vd2mdu	633	7	highest	high	JJS
cord-014597-66vd2mdu	633	8	viable	viable	JJ
cord-014597-66vd2mdu	633	9	cell	cell	NN
cord-014597-66vd2mdu	633	10	density	density	NN
cord-014597-66vd2mdu	633	11	(	(	-LRB-
cord-014597-66vd2mdu	633	12	VCD	VCD	NNP
cord-014597-66vd2mdu	633	13	)	)	-RRB-
cord-014597-66vd2mdu	633	14	,	,	,
cord-014597-66vd2mdu	633	15	followed	follow	VBN
cord-014597-66vd2mdu	633	16	by	by	IN
cord-014597-66vd2mdu	633	17	clone	clone	NN
cord-014597-66vd2mdu	633	18	#	#	$
cord-014597-66vd2mdu	633	19	1	1	CD
cord-014597-66vd2mdu	633	20	.	.	.
cord-014597-66vd2mdu	634	1	Clone	clone	NN
cord-014597-66vd2mdu	634	2	#	#	$
cord-014597-66vd2mdu	634	3	2	2	CD
cord-014597-66vd2mdu	634	4	and	and	CC
cord-014597-66vd2mdu	634	5	#	#	$
cord-014597-66vd2mdu	634	6	4	4	CD
cord-014597-66vd2mdu	634	7	showed	show	VBD
cord-014597-66vd2mdu	634	8	similar	similar	JJ
cord-014597-66vd2mdu	634	9	growth	growth	NN
cord-014597-66vd2mdu	634	10	characteristics	characteristic	NNS
cord-014597-66vd2mdu	634	11	.	.	.
cord-014597-66vd2mdu	635	1	It	-PRON-	PRP
cord-014597-66vd2mdu	635	2	is	be	VBZ
cord-014597-66vd2mdu	635	3	interesting	interesting	JJ
cord-014597-66vd2mdu	635	4	to	to	TO
cord-014597-66vd2mdu	635	5	note	note	VB
cord-014597-66vd2mdu	635	6	that	that	IN
cord-014597-66vd2mdu	635	7	in	in	IN
cord-014597-66vd2mdu	635	8	the	the	DT
cord-014597-66vd2mdu	635	9	perfusion	perfusion	NN
cord-014597-66vd2mdu	635	10	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	635	11	different	different	JJ
cord-014597-66vd2mdu	635	12	patterns	pattern	NNS
cord-014597-66vd2mdu	635	13	in	in	IN
cord-014597-66vd2mdu	635	14	terms	term	NNS
cord-014597-66vd2mdu	635	15	of	of	IN
cord-014597-66vd2mdu	635	16	VCD	vcd	NN
cord-014597-66vd2mdu	635	17	were	be	VBD
cord-014597-66vd2mdu	635	18	observed	observe	VBN
cord-014597-66vd2mdu	635	19	although	although	IN
cord-014597-66vd2mdu	635	20	the	the	DT
cord-014597-66vd2mdu	635	21	cell	cell	NN
cord-014597-66vd2mdu	635	22	biomass	biomass	NN
cord-014597-66vd2mdu	635	23	signals	signal	NNS
cord-014597-66vd2mdu	635	24	were	be	VBD
cord-014597-66vd2mdu	635	25	similar	similar	JJ
cord-014597-66vd2mdu	635	26	for	for	IN
cord-014597-66vd2mdu	635	27	all	all	DT
cord-014597-66vd2mdu	635	28	4	4	CD
cord-014597-66vd2mdu	635	29	runs	run	NNS
cord-014597-66vd2mdu	635	30	.	.	.
cord-014597-66vd2mdu	636	1	This	this	DT
cord-014597-66vd2mdu	636	2	reflects	reflect	VBZ
cord-014597-66vd2mdu	636	3	the	the	DT
cord-014597-66vd2mdu	636	4	fact	fact	NN
cord-014597-66vd2mdu	636	5	that	that	IN
cord-014597-66vd2mdu	636	6	the	the	DT
cord-014597-66vd2mdu	636	7	capacitance	capacitance	NN
cord-014597-66vd2mdu	636	8	measures	measure	VBZ
cord-014597-66vd2mdu	636	9	the	the	DT
cord-014597-66vd2mdu	636	10	biomass	biomass	NN
cord-014597-66vd2mdu	636	11	and	and	CC
cord-014597-66vd2mdu	636	12	not	not	RB
cord-014597-66vd2mdu	636	13	the	the	DT
cord-014597-66vd2mdu	636	14	absolute	absolute	JJ
cord-014597-66vd2mdu	636	15	cell	cell	NN
cord-014597-66vd2mdu	636	16	count	count	NN
cord-014597-66vd2mdu	636	17	[	[	-LRB-
cord-014597-66vd2mdu	636	18	5	5	CD
cord-014597-66vd2mdu	636	19	]	]	-RRB-
cord-014597-66vd2mdu	636	20	.	.	.
cord-014597-66vd2mdu	637	1	To	to	TO
cord-014597-66vd2mdu	637	2	estimate	estimate	VB
cord-014597-66vd2mdu	637	3	the	the	DT
cord-014597-66vd2mdu	637	4	minimum	minimum	JJ
cord-014597-66vd2mdu	637	5	cell	cell	NN
cord-014597-66vd2mdu	637	6	specific	specific	JJ
cord-014597-66vd2mdu	637	7	perfusion	perfusion	NN
cord-014597-66vd2mdu	637	8	rate	rate	NN
cord-014597-66vd2mdu	637	9	(	(	-LRB-
cord-014597-66vd2mdu	637	10	CSPR	CSPR	NNP
cord-014597-66vd2mdu	637	11	min	min	NNP
cord-014597-66vd2mdu	637	12	)	)	-RRB-
cord-014597-66vd2mdu	637	13	in	in	IN
cord-014597-66vd2mdu	637	14	the	the	DT
cord-014597-66vd2mdu	637	15	semi	semi	JJ
cord-014597-66vd2mdu	637	16	-	-	JJ
cord-014597-66vd2mdu	637	17	continuous	continuous	JJ
cord-014597-66vd2mdu	637	18	experiment	experiment	NN
cord-014597-66vd2mdu	637	19	,	,	,
cord-014597-66vd2mdu	637	20	the	the	DT
cord-014597-66vd2mdu	637	21	perfusion	perfusion	NN
cord-014597-66vd2mdu	637	22	rate	rate	NN
cord-014597-66vd2mdu	637	23	was	be	VBD
cord-014597-66vd2mdu	637	24	divided	divide	VBN
cord-014597-66vd2mdu	637	25	by	by	IN
cord-014597-66vd2mdu	637	26	the	the	DT
cord-014597-66vd2mdu	637	27	maximum	maximum	JJ
cord-014597-66vd2mdu	637	28	viable	viable	JJ
cord-014597-66vd2mdu	637	29	cell	cell	NN
cord-014597-66vd2mdu	637	30	density	density	NN
cord-014597-66vd2mdu	637	31	(	(	-LRB-
cord-014597-66vd2mdu	637	32	VCD	VCD	NNP
cord-014597-66vd2mdu	637	33	max	max	NNP
cord-014597-66vd2mdu	637	34	)	)	-RRB-
cord-014597-66vd2mdu	637	35	.	.	.
cord-014597-66vd2mdu	638	1	This	this	DT
cord-014597-66vd2mdu	638	2	value	value	NN
cord-014597-66vd2mdu	638	3	was	be	VBD
cord-014597-66vd2mdu	638	4	compared	compare	VBN
cord-014597-66vd2mdu	638	5	to	to	IN
cord-014597-66vd2mdu	638	6	the	the	DT
cord-014597-66vd2mdu	638	7	CSPR	CSPR	NNP
cord-014597-66vd2mdu	638	8	obtained	obtain	VBD
cord-014597-66vd2mdu	638	9	during	during	IN
cord-014597-66vd2mdu	638	10	the	the	DT
cord-014597-66vd2mdu	638	11	4	4	CD
cord-014597-66vd2mdu	638	12	th	th	XX
cord-014597-66vd2mdu	638	13	set	set	NN
cord-014597-66vd2mdu	638	14	-	-	HYPH
cord-014597-66vd2mdu	638	15	point	point	NN
cord-014597-66vd2mdu	638	16	(	(	-LRB-
cord-014597-66vd2mdu	638	17	SP4	SP4	NNP
cord-014597-66vd2mdu	638	18	)	)	-RRB-
cord-014597-66vd2mdu	638	19	of	of	IN
cord-014597-66vd2mdu	638	20	the	the	DT
cord-014597-66vd2mdu	638	21	perfusion	perfusion	NN
cord-014597-66vd2mdu	638	22	runs	run	NNS
cord-014597-66vd2mdu	638	23	.	.	.
cord-014597-66vd2mdu	639	1	As	as	IN
cord-014597-66vd2mdu	639	2	expected	expect	VBN
cord-014597-66vd2mdu	639	3	,	,	,
cord-014597-66vd2mdu	639	4	the	the	DT
cord-014597-66vd2mdu	639	5	bleed	bleed	NN
cord-014597-66vd2mdu	639	6	fraction	fraction	NN
cord-014597-66vd2mdu	639	7	decreased	decrease	VBD
cord-014597-66vd2mdu	639	8	when	when	WRB
cord-014597-66vd2mdu	639	9	the	the	DT
cord-014597-66vd2mdu	639	10	capacitance	capacitance	NN
cord-014597-66vd2mdu	639	11	set	set	NN
cord-014597-66vd2mdu	639	12	-	-	HYPH
cord-014597-66vd2mdu	639	13	point	point	NN
cord-014597-66vd2mdu	639	14	was	be	VBD
cord-014597-66vd2mdu	639	15	increased	increase	VBN
cord-014597-66vd2mdu	639	16	and	and	CC
cord-014597-66vd2mdu	639	17	went	go	VBD
cord-014597-66vd2mdu	639	18	down	down	RB
cord-014597-66vd2mdu	639	19	to	to	IN
cord-014597-66vd2mdu	639	20	5	5	CD
cord-014597-66vd2mdu	639	21	%	%	NN
cord-014597-66vd2mdu	639	22	or	or	CC
cord-014597-66vd2mdu	639	23	less	less	JJR
cord-014597-66vd2mdu	639	24	of	of	IN
cord-014597-66vd2mdu	639	25	the	the	DT
cord-014597-66vd2mdu	639	26	total	total	JJ
cord-014597-66vd2mdu	639	27	perfusion	perfusion	NN
cord-014597-66vd2mdu	639	28	rate	rate	NN
cord-014597-66vd2mdu	639	29	(	(	-LRB-
cord-014597-66vd2mdu	639	30	data	datum	NNS
cord-014597-66vd2mdu	639	31	not	not	RB
cord-014597-66vd2mdu	639	32	shown	show	VBN
cord-014597-66vd2mdu	639	33	)	)	-RRB-
cord-014597-66vd2mdu	639	34	.	.	.
cord-014597-66vd2mdu	640	1	Since	since	IN
cord-014597-66vd2mdu	640	2	the	the	DT
cord-014597-66vd2mdu	640	3	bleed	bleed	NN
cord-014597-66vd2mdu	640	4	removes	remove	VBZ
cord-014597-66vd2mdu	640	5	the	the	DT
cord-014597-66vd2mdu	640	6	excess	excess	JJ
cord-014597-66vd2mdu	640	7	biomass	biomass	NN
cord-014597-66vd2mdu	640	8	,	,	,
cord-014597-66vd2mdu	640	9	it	-PRON-	PRP
cord-014597-66vd2mdu	640	10	is	be	VBZ
cord-014597-66vd2mdu	640	11	an	an	DT
cord-014597-66vd2mdu	640	12	indication	indication	NN
cord-014597-66vd2mdu	640	13	of	of	IN
cord-014597-66vd2mdu	640	14	how	how	WRB
cord-014597-66vd2mdu	640	15	close	close	JJ
cord-014597-66vd2mdu	640	16	to	to	IN
cord-014597-66vd2mdu	640	17	a	a	DT
cord-014597-66vd2mdu	640	18	limitation	limitation	NN
cord-014597-66vd2mdu	640	19	the	the	DT
cord-014597-66vd2mdu	640	20	system	system	NN
cord-014597-66vd2mdu	640	21	is	be	VBZ
cord-014597-66vd2mdu	640	22	.	.	.
cord-014597-66vd2mdu	641	1	Therefore	therefore	RB
cord-014597-66vd2mdu	641	2	,	,	,
cord-014597-66vd2mdu	641	3	the	the	DT
cord-014597-66vd2mdu	641	4	CSPR	CSPR	NNP
cord-014597-66vd2mdu	641	5	calculated	calculate	VBN
cord-014597-66vd2mdu	641	6	at	at	IN
cord-014597-66vd2mdu	641	7	SP4	SP4	NNP
cord-014597-66vd2mdu	641	8	was	be	VBD
cord-014597-66vd2mdu	641	9	considered	consider	VBN
cord-014597-66vd2mdu	641	10	as	as	IN
cord-014597-66vd2mdu	641	11	the	the	DT
cord-014597-66vd2mdu	641	12	minimum	minimum	JJ
cord-014597-66vd2mdu	641	13	CSPR	CSPR	NNP
cord-014597-66vd2mdu	641	14	.	.	.
cord-014597-66vd2mdu	642	1	The	the	DT
cord-014597-66vd2mdu	642	2	CSPR	CSPR	NNP
cord-014597-66vd2mdu	642	3	min	min	NN
cord-014597-66vd2mdu	642	4	obtained	obtain	VBD
cord-014597-66vd2mdu	642	5	in	in	IN
cord-014597-66vd2mdu	642	6	both	both	DT
cord-014597-66vd2mdu	642	7	systems	system	NNS
cord-014597-66vd2mdu	642	8	were	be	VBD
cord-014597-66vd2mdu	642	9	very	very	RB
cord-014597-66vd2mdu	642	10	close	close	JJ
cord-014597-66vd2mdu	642	11	(	(	-LRB-
cord-014597-66vd2mdu	642	12	Table	table	NN
cord-014597-66vd2mdu	642	13	1	1	CD
cord-014597-66vd2mdu	642	14	)	)	-RRB-
cord-014597-66vd2mdu	642	15	.	.	.
cord-014597-66vd2mdu	643	1	The	the	DT
cord-014597-66vd2mdu	643	2	semi	semi	JJ
cord-014597-66vd2mdu	643	3	-	-	JJ
cord-014597-66vd2mdu	643	4	continuous	continuous	JJ
cord-014597-66vd2mdu	643	5	system	system	NN
cord-014597-66vd2mdu	643	6	can	can	MD
cord-014597-66vd2mdu	643	7	therefore	therefore	RB
cord-014597-66vd2mdu	643	8	be	be	VB
cord-014597-66vd2mdu	643	9	used	use	VBN
cord-014597-66vd2mdu	643	10	to	to	TO
cord-014597-66vd2mdu	643	11	identify	identify	VB
cord-014597-66vd2mdu	643	12	the	the	DT
cord-014597-66vd2mdu	643	13	CSPR	CSPR	NNP
cord-014597-66vd2mdu	643	14	min	min	NNP
cord-014597-66vd2mdu	643	15	before	before	IN
cord-014597-66vd2mdu	643	16	running	run	VBG
cord-014597-66vd2mdu	643	17	a	a	DT
cord-014597-66vd2mdu	643	18	continuous	continuous	JJ
cord-014597-66vd2mdu	643	19	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	643	20	,	,	,
cord-014597-66vd2mdu	643	21	it	-PRON-	PRP
cord-014597-66vd2mdu	643	22	therefore	therefore	RB
cord-014597-66vd2mdu	643	23	facilitates	facilitate	VBZ
cord-014597-66vd2mdu	643	24	the	the	DT
cord-014597-66vd2mdu	643	25	decision	decision	NN
cord-014597-66vd2mdu	643	26	making	make	VBG
cord-014597-66vd2mdu	643	27	early	early	RB
cord-014597-66vd2mdu	643	28	in	in	IN
cord-014597-66vd2mdu	643	29	the	the	DT
cord-014597-66vd2mdu	643	30	development	development	NN
cord-014597-66vd2mdu	643	31	(	(	-LRB-
cord-014597-66vd2mdu	643	32	to	to	TO
cord-014597-66vd2mdu	643	33	define	define	VB
cord-014597-66vd2mdu	643	34	the	the	DT
cord-014597-66vd2mdu	643	35	target	target	NN
cord-014597-66vd2mdu	643	36	cell	cell	NN
cord-014597-66vd2mdu	643	37	density	density	NN
cord-014597-66vd2mdu	643	38	for	for	IN
cord-014597-66vd2mdu	643	39	a	a	DT
cord-014597-66vd2mdu	643	40	defined	define	VBN
cord-014597-66vd2mdu	643	41	perfusion	perfusion	NN
cord-014597-66vd2mdu	643	42	rate	rate	NN
cord-014597-66vd2mdu	643	43	)	)	-RRB-
cord-014597-66vd2mdu	643	44	.	.	.
cord-014597-66vd2mdu	644	1	The	the	DT
cord-014597-66vd2mdu	644	2	specific	specific	JJ
cord-014597-66vd2mdu	644	3	productivity	productivity	NN
cord-014597-66vd2mdu	644	4	(	(	-LRB-
cord-014597-66vd2mdu	644	5	Q	q	NN
cord-014597-66vd2mdu	644	6	p	p	NN
cord-014597-66vd2mdu	644	7	)	)	-RRB-
cord-014597-66vd2mdu	644	8	of	of	IN
cord-014597-66vd2mdu	644	9	the	the	DT
cord-014597-66vd2mdu	644	10	4	4	CD
cord-014597-66vd2mdu	644	11	clones	clone	NNS
cord-014597-66vd2mdu	644	12	was	be	VBD
cord-014597-66vd2mdu	644	13	quantified	quantify	VBN
cord-014597-66vd2mdu	644	14	at	at	IN
cord-014597-66vd2mdu	644	15	the	the	DT
cord-014597-66vd2mdu	644	16	maximum	maximum	JJ
cord-014597-66vd2mdu	644	17	VCD	vcd	NN
cord-014597-66vd2mdu	644	18	(	(	-LRB-
cord-014597-66vd2mdu	644	19	semi	semi	JJ
cord-014597-66vd2mdu	644	20	-	-	JJ
cord-014597-66vd2mdu	644	21	continuous	continuous	JJ
cord-014597-66vd2mdu	644	22	)	)	-RRB-
cord-014597-66vd2mdu	644	23	or	or	CC
cord-014597-66vd2mdu	644	24	at	at	IN
cord-014597-66vd2mdu	644	25	SP4	SP4	NNP
cord-014597-66vd2mdu	644	26	(	(	-LRB-
cord-014597-66vd2mdu	644	27	perfusion	perfusion	NN
cord-014597-66vd2mdu	644	28	)	)	-RRB-
cord-014597-66vd2mdu	644	29	.	.	.
cord-014597-66vd2mdu	645	1	Absolute	absolute	JJ
cord-014597-66vd2mdu	645	2	values	value	NNS
cord-014597-66vd2mdu	645	3	are	be	VBP
cord-014597-66vd2mdu	645	4	not	not	RB
cord-014597-66vd2mdu	645	5	representative	representative	JJ
cord-014597-66vd2mdu	645	6	since	since	IN
cord-014597-66vd2mdu	645	7	the	the	DT
cord-014597-66vd2mdu	645	8	cell	cell	NN
cord-014597-66vd2mdu	645	9	environment	environment	NN
cord-014597-66vd2mdu	645	10	is	be	VBZ
cord-014597-66vd2mdu	645	11	so	so	RB
cord-014597-66vd2mdu	645	12	different	different	JJ
cord-014597-66vd2mdu	645	13	in	in	IN
cord-014597-66vd2mdu	645	14	both	both	DT
cord-014597-66vd2mdu	645	15	systems	system	NNS
cord-014597-66vd2mdu	645	16	.	.	.
cord-014597-66vd2mdu	646	1	Nevertheless	nevertheless	RB
cord-014597-66vd2mdu	646	2	,	,	,
cord-014597-66vd2mdu	646	3	a	a	DT
cord-014597-66vd2mdu	646	4	relative	relative	JJ
cord-014597-66vd2mdu	646	5	ranking	ranking	NN
cord-014597-66vd2mdu	646	6	proved	prove	VBD
cord-014597-66vd2mdu	646	7	to	to	TO
cord-014597-66vd2mdu	646	8	be	be	VB
cord-014597-66vd2mdu	646	9	indicative	indicative	JJ
cord-014597-66vd2mdu	646	10	of	of	IN
cord-014597-66vd2mdu	646	11	the	the	DT
cord-014597-66vd2mdu	646	12	respective	respective	JJ
cord-014597-66vd2mdu	646	13	performances	performance	NNS
cord-014597-66vd2mdu	646	14	(	(	-LRB-
cord-014597-66vd2mdu	646	15	Table	table	NN
cord-014597-66vd2mdu	646	16	1	1	CD
cord-014597-66vd2mdu	646	17	)	)	-RRB-
cord-014597-66vd2mdu	646	18	.	.	.
cord-014597-66vd2mdu	647	1	The	the	DT
cord-014597-66vd2mdu	647	2	maximum	maximum	JJ
cord-014597-66vd2mdu	647	3	cell	cell	NN
cord-014597-66vd2mdu	647	4	growth	growth	NN
cord-014597-66vd2mdu	647	5	in	in	IN
cord-014597-66vd2mdu	647	6	fed	fed	NNP
cord-014597-66vd2mdu	647	7	-	-	HYPH
cord-014597-66vd2mdu	647	8	batch	batch	NN
cord-014597-66vd2mdu	647	9	,	,	,
cord-014597-66vd2mdu	647	10	semi	semi	JJ
cord-014597-66vd2mdu	647	11	-	-	JJ
cord-014597-66vd2mdu	647	12	continuous	continuous	JJ
cord-014597-66vd2mdu	647	13	and	and	CC
cord-014597-66vd2mdu	647	14	perfusion	perfusion	NN
cord-014597-66vd2mdu	647	15	were	be	VBD
cord-014597-66vd2mdu	647	16	also	also	RB
cord-014597-66vd2mdu	647	17	compared	compare	VBN
cord-014597-66vd2mdu	647	18	,	,	,
cord-014597-66vd2mdu	647	19	their	-PRON-	PRP$
cord-014597-66vd2mdu	647	20	ranking	ranking	NN
cord-014597-66vd2mdu	647	21	was	be	VBD
cord-014597-66vd2mdu	647	22	always	always	RB
cord-014597-66vd2mdu	647	23	preserved	preserve	VBN
cord-014597-66vd2mdu	647	24	.	.	.
cord-014597-66vd2mdu	648	1	Both	both	DT
cord-014597-66vd2mdu	648	2	indications	indication	NNS
cord-014597-66vd2mdu	648	3	can	can	MD
cord-014597-66vd2mdu	648	4	be	be	VB
cord-014597-66vd2mdu	648	5	used	use	VBN
cord-014597-66vd2mdu	648	6	to	to	TO
cord-014597-66vd2mdu	648	7	assess	assess	VB
cord-014597-66vd2mdu	648	8	a	a	DT
cord-014597-66vd2mdu	648	9	performance	performance	NN
cord-014597-66vd2mdu	648	10	ranking	ranking	NN
cord-014597-66vd2mdu	648	11	for	for	IN
cord-014597-66vd2mdu	648	12	different	different	JJ
cord-014597-66vd2mdu	648	13	clones	clone	NNS
cord-014597-66vd2mdu	648	14	.	.	.
cord-014597-66vd2mdu	649	1	The	the	DT
cord-014597-66vd2mdu	649	2	performance	performance	NN
cord-014597-66vd2mdu	649	3	of	of	IN
cord-014597-66vd2mdu	649	4	4	4	CD
cord-014597-66vd2mdu	649	5	clones	clone	NNS
cord-014597-66vd2mdu	649	6	was	be	VBD
cord-014597-66vd2mdu	649	7	studied	study	VBN
cord-014597-66vd2mdu	649	8	in	in	IN
cord-014597-66vd2mdu	649	9	3	3	CD
cord-014597-66vd2mdu	649	10	different	different	JJ
cord-014597-66vd2mdu	649	11	cultivation	cultivation	NN
cord-014597-66vd2mdu	649	12	systems	system	NNS
cord-014597-66vd2mdu	649	13	:	:	:
cord-014597-66vd2mdu	649	14	fed	feed	VBN
cord-014597-66vd2mdu	649	15	-	-	HYPH
cord-014597-66vd2mdu	649	16	batch	batch	NN
cord-014597-66vd2mdu	649	17	/	/	SYM
cord-014597-66vd2mdu	649	18	perfusion	perfusion	NN
cord-014597-66vd2mdu	649	19	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	649	20	and	and	CC
cord-014597-66vd2mdu	649	21	semi	semi	JJ
cord-014597-66vd2mdu	649	22	-	-	JJ
cord-014597-66vd2mdu	649	23	continuous	continuous	JJ
cord-014597-66vd2mdu	649	24	shake	shake	NN
cord-014597-66vd2mdu	649	25	tube	tube	NN
cord-014597-66vd2mdu	649	26	.	.	.
cord-014597-66vd2mdu	650	1	The	the	DT
cord-014597-66vd2mdu	650	2	semi	semi	JJ
cord-014597-66vd2mdu	650	3	-	-	JJ
cord-014597-66vd2mdu	650	4	continuous	continuous	JJ
cord-014597-66vd2mdu	650	5	system	system	NN
cord-014597-66vd2mdu	650	6	was	be	VBD
cord-014597-66vd2mdu	650	7	able	able	JJ
cord-014597-66vd2mdu	650	8	to	to	TO
cord-014597-66vd2mdu	650	9	precisely	precisely	RB
cord-014597-66vd2mdu	650	10	predict	predict	VB
cord-014597-66vd2mdu	650	11	the	the	DT
cord-014597-66vd2mdu	650	12	CSPR	CSPR	NNP
cord-014597-66vd2mdu	650	13	min	min	NNP
cord-014597-66vd2mdu	650	14	,	,	,
cord-014597-66vd2mdu	650	15	an	an	DT
cord-014597-66vd2mdu	650	16	important	important	JJ
cord-014597-66vd2mdu	650	17	process	process	NN
cord-014597-66vd2mdu	650	18	parameter	parameter	NN
cord-014597-66vd2mdu	650	19	for	for	IN
cord-014597-66vd2mdu	650	20	perfusion	perfusion	NN
cord-014597-66vd2mdu	650	21	.	.	.
cord-014597-66vd2mdu	651	1	Specific	specific	JJ
cord-014597-66vd2mdu	651	2	productivity	productivity	NN
cord-014597-66vd2mdu	651	3	and	and	CC
cord-014597-66vd2mdu	651	4	maximum	maximum	JJ
cord-014597-66vd2mdu	651	5	cell	cell	NN
cord-014597-66vd2mdu	651	6	density	density	NN
cord-014597-66vd2mdu	651	7	ranking	ranking	NN
cord-014597-66vd2mdu	651	8	was	be	VBD
cord-014597-66vd2mdu	651	9	preserved	preserve	VBN
cord-014597-66vd2mdu	651	10	across	across	IN
cord-014597-66vd2mdu	651	11	the	the	DT
cord-014597-66vd2mdu	651	12	systems	system	NNS
cord-014597-66vd2mdu	651	13	,	,	,
cord-014597-66vd2mdu	651	14	therefore	therefore	RB
cord-014597-66vd2mdu	651	15	the	the	DT
cord-014597-66vd2mdu	651	16	scale	scale	NN
cord-014597-66vd2mdu	651	17	down	down	RP
cord-014597-66vd2mdu	651	18	experiment	experiment	NN
cord-014597-66vd2mdu	651	19	can	can	MD
cord-014597-66vd2mdu	651	20	be	be	VB
cord-014597-66vd2mdu	651	21	used	use	VBN
cord-014597-66vd2mdu	651	22	to	to	TO
cord-014597-66vd2mdu	651	23	assess	assess	VB
cord-014597-66vd2mdu	651	24	a	a	DT
cord-014597-66vd2mdu	651	25	performance	performance	NN
cord-014597-66vd2mdu	651	26	ranking	rank	VBG
cord-014597-66vd2mdu	651	27	for	for	IN
cord-014597-66vd2mdu	651	28	perfusion	perfusion	NN
cord-014597-66vd2mdu	651	29	clone	clone	NN
cord-014597-66vd2mdu	651	30	screening	screening	NN
cord-014597-66vd2mdu	651	31	.	.	.
cord-014597-66vd2mdu	652	1	Modulating	modulate	VBG
cord-014597-66vd2mdu	652	2	antibody	antibody	NN
cord-014597-66vd2mdu	652	3	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	652	4	through	through	IN
cord-014597-66vd2mdu	652	5	cell	cell	NN
cord-014597-66vd2mdu	652	6	culture	culture	NN
cord-014597-66vd2mdu	652	7	medium	medium	NN
cord-014597-66vd2mdu	652	8	for	for	IN
cord-014597-66vd2mdu	652	9	improved	improved	JJ
cord-014597-66vd2mdu	652	10	function	function	NN
cord-014597-66vd2mdu	652	11	and	and	CC
cord-014597-66vd2mdu	652	12	product	product	NN
cord-014597-66vd2mdu	652	13	quality	quality	NN
cord-014597-66vd2mdu	653	1	Jenny	Jenny	NNP
cord-014597-66vd2mdu	653	2	Y.	Y.	NNP
cord-014597-66vd2mdu	653	3	Bang	Bang	NNP
cord-014597-66vd2mdu	653	4	,	,	,
cord-014597-66vd2mdu	653	5	James	James	NNP
cord-014597-66vd2mdu	653	6	-	-	HYPH
cord-014597-66vd2mdu	653	7	Kevin	Kevin	NNP
cord-014597-66vd2mdu	653	8	Y.	Y.	NNP
cord-014597-66vd2mdu	653	9	Tan	Tan	NNP
cord-014597-66vd2mdu	654	1	The	the	DT
cord-014597-66vd2mdu	654	2	production	production	NN
cord-014597-66vd2mdu	654	3	of	of	IN
cord-014597-66vd2mdu	654	4	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	654	5	antibodies	antibody	NNS
cord-014597-66vd2mdu	654	6	(	(	-LRB-
cord-014597-66vd2mdu	654	7	Abs	abs	NN
cord-014597-66vd2mdu	654	8	)	)	-RRB-
cord-014597-66vd2mdu	654	9	requires	require	VBZ
cord-014597-66vd2mdu	654	10	high	high	JJ
cord-014597-66vd2mdu	654	11	titers	titer	NNS
cord-014597-66vd2mdu	654	12	and	and	CC
cord-014597-66vd2mdu	654	13	excellent	excellent	JJ
cord-014597-66vd2mdu	654	14	product	product	NN
cord-014597-66vd2mdu	654	15	quality	quality	NN
cord-014597-66vd2mdu	654	16	to	to	TO
cord-014597-66vd2mdu	654	17	ensure	ensure	VB
cord-014597-66vd2mdu	654	18	efficient	efficient	JJ
cord-014597-66vd2mdu	654	19	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	654	20	and	and	CC
cord-014597-66vd2mdu	654	21	potent	potent	JJ
cord-014597-66vd2mdu	654	22	drug	drug	NN
cord-014597-66vd2mdu	654	23	efficacy	efficacy	NN
cord-014597-66vd2mdu	654	24	.	.	.
cord-014597-66vd2mdu	655	1	Glycosylation	Glycosylation	NNP
cord-014597-66vd2mdu	655	2	,	,	,
cord-014597-66vd2mdu	655	3	or	or	CC
cord-014597-66vd2mdu	655	4	the	the	DT
cord-014597-66vd2mdu	655	5	attachment	attachment	NN
cord-014597-66vd2mdu	655	6	of	of	IN
cord-014597-66vd2mdu	655	7	sugars	sugar	NNS
cord-014597-66vd2mdu	655	8	to	to	IN
cord-014597-66vd2mdu	655	9	organic	organic	JJ
cord-014597-66vd2mdu	655	10	molecules	molecule	NNS
cord-014597-66vd2mdu	655	11	,	,	,
cord-014597-66vd2mdu	655	12	is	be	VBZ
cord-014597-66vd2mdu	655	13	a	a	DT
cord-014597-66vd2mdu	655	14	critical	critical	JJ
cord-014597-66vd2mdu	655	15	quality	quality	NN
cord-014597-66vd2mdu	655	16	aspect	aspect	NN
cord-014597-66vd2mdu	655	17	that	that	WDT
cord-014597-66vd2mdu	655	18	can	can	MD
cord-014597-66vd2mdu	655	19	significantly	significantly	RB
cord-014597-66vd2mdu	655	20	alter	alter	VB
cord-014597-66vd2mdu	655	21	Ab	Ab	NNP
cord-014597-66vd2mdu	655	22	binding	binding	NN
cord-014597-66vd2mdu	655	23	,	,	,
cord-014597-66vd2mdu	655	24	function	function	NN
cord-014597-66vd2mdu	655	25	,	,	,
cord-014597-66vd2mdu	655	26	and	and	CC
cord-014597-66vd2mdu	655	27	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	655	28	effect	effect	NN
cord-014597-66vd2mdu	655	29	[	[	-LRB-
cord-014597-66vd2mdu	655	30	1	1	CD
cord-014597-66vd2mdu	655	31	]	]	-RRB-
cord-014597-66vd2mdu	655	32	.	.	.
cord-014597-66vd2mdu	656	1	Galactose	galactose	NN
cord-014597-66vd2mdu	656	2	is	be	VBZ
cord-014597-66vd2mdu	656	3	a	a	DT
cord-014597-66vd2mdu	656	4	key	key	JJ
cord-014597-66vd2mdu	656	5	sugar	sugar	NN
cord-014597-66vd2mdu	656	6	of	of	IN
cord-014597-66vd2mdu	656	7	interest	interest	NN
cord-014597-66vd2mdu	656	8	due	due	IN
cord-014597-66vd2mdu	656	9	to	to	IN
cord-014597-66vd2mdu	656	10	its	-PRON-	PRP$
cord-014597-66vd2mdu	656	11	significant	significant	JJ
cord-014597-66vd2mdu	656	12	impact	impact	NN
cord-014597-66vd2mdu	656	13	on	on	IN
cord-014597-66vd2mdu	656	14	Ab	Ab	NNP
cord-014597-66vd2mdu	656	15	function	function	NN
cord-014597-66vd2mdu	656	16	and	and	CC
cord-014597-66vd2mdu	656	17	the	the	DT
cord-014597-66vd2mdu	656	18	ability	ability	NN
cord-014597-66vd2mdu	656	19	to	to	TO
cord-014597-66vd2mdu	656	20	control	control	VB
cord-014597-66vd2mdu	656	21	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	656	22	through	through	IN
cord-014597-66vd2mdu	656	23	cell	cell	NN
cord-014597-66vd2mdu	656	24	culture	culture	NN
cord-014597-66vd2mdu	656	25	medium	medium	NN
cord-014597-66vd2mdu	656	26	.	.	.
cord-014597-66vd2mdu	657	1	Herein	herein	RB
cord-014597-66vd2mdu	657	2	,	,	,
cord-014597-66vd2mdu	657	3	Irvine	Irvine	NNP
cord-014597-66vd2mdu	657	4	Scientific	Scientific	NNP
cord-014597-66vd2mdu	657	5	assessed	assess	VBD
cord-014597-66vd2mdu	657	6	the	the	DT
cord-014597-66vd2mdu	657	7	ability	ability	NN
cord-014597-66vd2mdu	657	8	of	of	IN
cord-014597-66vd2mdu	657	9	media	medium	NNS
cord-014597-66vd2mdu	657	10	components	component	NNS
cord-014597-66vd2mdu	657	11	to	to	TO
cord-014597-66vd2mdu	657	12	modulate	modulate	VB
cord-014597-66vd2mdu	657	13	galactose	galactose	NN
cord-014597-66vd2mdu	657	14	levels	level	NNS
cord-014597-66vd2mdu	657	15	on	on	IN
cord-014597-66vd2mdu	657	16	a	a	DT
cord-014597-66vd2mdu	657	17	model	model	NN
cord-014597-66vd2mdu	657	18	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	657	19	Ab	ab	NN
cord-014597-66vd2mdu	657	20	.	.	.
cord-014597-66vd2mdu	658	1	Various	various	JJ
cord-014597-66vd2mdu	658	2	media	medium	NNS
cord-014597-66vd2mdu	658	3	compositions	composition	NNS
cord-014597-66vd2mdu	658	4	were	be	VBD
cord-014597-66vd2mdu	658	5	able	able	JJ
cord-014597-66vd2mdu	658	6	to	to	TO
cord-014597-66vd2mdu	658	7	modulate	modulate	VB
cord-014597-66vd2mdu	658	8	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	658	9	levels	level	NNS
cord-014597-66vd2mdu	658	10	without	without	IN
cord-014597-66vd2mdu	658	11	compromising	compromise	VBG
cord-014597-66vd2mdu	658	12	cell	cell	NN
cord-014597-66vd2mdu	658	13	growth	growth	NN
cord-014597-66vd2mdu	658	14	and	and	CC
cord-014597-66vd2mdu	658	15	Ab	Ab	NNP
cord-014597-66vd2mdu	658	16	titers	titer	NNS
cord-014597-66vd2mdu	658	17	.	.	.
cord-014597-66vd2mdu	659	1	In	in	IN
cord-014597-66vd2mdu	659	2	addition	addition	NN
cord-014597-66vd2mdu	659	3	,	,	,
cord-014597-66vd2mdu	659	4	an	an	DT
cord-014597-66vd2mdu	659	5	in	in	FW
cord-014597-66vd2mdu	659	6	vitro	vitro	FW
cord-014597-66vd2mdu	659	7	assay	assay	NN
cord-014597-66vd2mdu	659	8	was	be	VBD
cord-014597-66vd2mdu	659	9	utilized	utilize	VBN
cord-014597-66vd2mdu	659	10	to	to	TO
cord-014597-66vd2mdu	659	11	evaluate	evaluate	VB
cord-014597-66vd2mdu	659	12	the	the	DT
cord-014597-66vd2mdu	659	13	functional	functional	JJ
cord-014597-66vd2mdu	659	14	ability	ability	NN
cord-014597-66vd2mdu	659	15	of	of	IN
cord-014597-66vd2mdu	659	16	Abs	abs	NN
cord-014597-66vd2mdu	659	17	to	to	TO
cord-014597-66vd2mdu	659	18	bind	bind	VB
cord-014597-66vd2mdu	659	19	and	and	CC
cord-014597-66vd2mdu	659	20	activate	activate	VB
cord-014597-66vd2mdu	659	21	complement	complement	NN
cord-014597-66vd2mdu	659	22	-	-	HYPH
cord-014597-66vd2mdu	659	23	dependent	dependent	JJ
cord-014597-66vd2mdu	659	24	cytotoxicity	cytotoxicity	NN
cord-014597-66vd2mdu	659	25	(	(	-LRB-
cord-014597-66vd2mdu	659	26	CDC	CDC	NNP
cord-014597-66vd2mdu	659	27	)	)	-RRB-
cord-014597-66vd2mdu	659	28	.	.	.
cord-014597-66vd2mdu	660	1	Differences	difference	NNS
cord-014597-66vd2mdu	660	2	in	in	IN
cord-014597-66vd2mdu	660	3	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	660	4	significantly	significantly	RB
cord-014597-66vd2mdu	660	5	altered	alter	VBD
cord-014597-66vd2mdu	660	6	the	the	DT
cord-014597-66vd2mdu	660	7	Abs	abs	NN
cord-014597-66vd2mdu	660	8	'	'	POS
cord-014597-66vd2mdu	660	9	ability	ability	NN
cord-014597-66vd2mdu	660	10	to	to	TO
cord-014597-66vd2mdu	660	11	induce	induce	VB
cord-014597-66vd2mdu	660	12	cell	cell	NN
cord-014597-66vd2mdu	660	13	cytotoxicity	cytotoxicity	NN
cord-014597-66vd2mdu	660	14	.	.	.
cord-014597-66vd2mdu	661	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	661	2	,	,	,
cord-014597-66vd2mdu	661	3	design	design	NN
cord-014597-66vd2mdu	661	4	of	of	IN
cord-014597-66vd2mdu	661	5	experiment	experiment	NN
cord-014597-66vd2mdu	661	6	analysis	analysis	NN
cord-014597-66vd2mdu	661	7	determined	determine	VBD
cord-014597-66vd2mdu	661	8	the	the	DT
cord-014597-66vd2mdu	661	9	optimal	optimal	JJ
cord-014597-66vd2mdu	661	10	ratio	ratio	NN
cord-014597-66vd2mdu	661	11	of	of	IN
cord-014597-66vd2mdu	661	12	supplements	supplement	NNS
cord-014597-66vd2mdu	661	13	to	to	TO
cord-014597-66vd2mdu	661	14	maximize	maximize	VB
cord-014597-66vd2mdu	661	15	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	661	16	.	.	.
cord-014597-66vd2mdu	662	1	This	this	DT
cord-014597-66vd2mdu	662	2	"	"	``
cord-014597-66vd2mdu	662	3	Optimized	optimize	VBN
cord-014597-66vd2mdu	662	4	Supplement	supplement	NN
cord-014597-66vd2mdu	662	5	"	"	''
cord-014597-66vd2mdu	662	6	was	be	VBD
cord-014597-66vd2mdu	662	7	verified	verify	VBN
cord-014597-66vd2mdu	662	8	and	and	CC
cord-014597-66vd2mdu	662	9	evaluated	evaluate	VBN
cord-014597-66vd2mdu	662	10	against	against	IN
cord-014597-66vd2mdu	662	11	other	other	JJ
cord-014597-66vd2mdu	662	12	suppliers	supplier	NNS
cord-014597-66vd2mdu	662	13	'	'	POS
cord-014597-66vd2mdu	662	14	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	662	15	supplements	supplement	NNS
cord-014597-66vd2mdu	662	16	in	in	IN
cord-014597-66vd2mdu	662	17	terms	term	NNS
cord-014597-66vd2mdu	662	18	of	of	IN
cord-014597-66vd2mdu	662	19	growth	growth	NN
cord-014597-66vd2mdu	662	20	,	,	,
cord-014597-66vd2mdu	662	21	titer	titer	NN
cord-014597-66vd2mdu	662	22	,	,	,
cord-014597-66vd2mdu	662	23	glycan	glycan	NN
cord-014597-66vd2mdu	662	24	analysis	analysis	NN
cord-014597-66vd2mdu	662	25	,	,	,
cord-014597-66vd2mdu	662	26	and	and	CC
cord-014597-66vd2mdu	662	27	Ab	Ab	NNP
cord-014597-66vd2mdu	662	28	function	function	NN
cord-014597-66vd2mdu	662	29	.	.	.
cord-014597-66vd2mdu	663	1	The	the	DT
cord-014597-66vd2mdu	663	2	Optimized	Optimized	NNP
cord-014597-66vd2mdu	663	3	Supplement	Supplement	NNP
cord-014597-66vd2mdu	663	4	outperformed	outperform	VBD
cord-014597-66vd2mdu	663	5	all	all	DT
cord-014597-66vd2mdu	663	6	other	other	JJ
cord-014597-66vd2mdu	663	7	suppliers	supplier	NNS
cord-014597-66vd2mdu	663	8	'	'	POS
cord-014597-66vd2mdu	663	9	supplements	supplement	NNS
cord-014597-66vd2mdu	663	10	and	and	CC
cord-014597-66vd2mdu	663	11	resulted	result	VBD
cord-014597-66vd2mdu	663	12	in	in	IN
cord-014597-66vd2mdu	663	13	the	the	DT
cord-014597-66vd2mdu	663	14	best	good	JJS
cord-014597-66vd2mdu	663	15	overall	overall	JJ
cord-014597-66vd2mdu	663	16	cell	cell	NN
cord-014597-66vd2mdu	663	17	growth	growth	NN
cord-014597-66vd2mdu	663	18	,	,	,
cord-014597-66vd2mdu	663	19	titer	titer	NN
cord-014597-66vd2mdu	663	20	,	,	,
cord-014597-66vd2mdu	663	21	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	663	22	,	,	,
cord-014597-66vd2mdu	663	23	and	and	CC
cord-014597-66vd2mdu	663	24	Ab	Ab	NNP
cord-014597-66vd2mdu	663	25	function	function	NN
cord-014597-66vd2mdu	663	26	.	.	.
cord-014597-66vd2mdu	664	1	Ab	Ab	NNP
cord-014597-66vd2mdu	664	2	against	against	IN
cord-014597-66vd2mdu	664	3	CD20	CD20	NNP
cord-014597-66vd2mdu	664	4	were	be	VBD
cord-014597-66vd2mdu	664	5	grown	grow	VBN
cord-014597-66vd2mdu	664	6	in	in	IN
cord-014597-66vd2mdu	664	7	BalanCD	BalanCD	NNP
cord-014597-66vd2mdu	664	8	®	®	POS
cord-014597-66vd2mdu	664	9	CHO	CHO	NNP
cord-014597-66vd2mdu	664	10	Growth	Growth	NNP
cord-014597-66vd2mdu	664	11	A	A	NNP
cord-014597-66vd2mdu	664	12	and	and	CC
cord-014597-66vd2mdu	664	13	were	be	VBD
cord-014597-66vd2mdu	664	14	fed	feed	VBN
cord-014597-66vd2mdu	664	15	with	with	IN
cord-014597-66vd2mdu	664	16	BalanCD	BalanCD	NNP
cord-014597-66vd2mdu	664	17	®	®	POS
cord-014597-66vd2mdu	664	18	CHO	CHO	NNP
cord-014597-66vd2mdu	664	19	Feed	Feed	NNP
cord-014597-66vd2mdu	664	20	4	4	CD
cord-014597-66vd2mdu	664	21	on	on	IN
cord-014597-66vd2mdu	664	22	days	day	NNS
cord-014597-66vd2mdu	664	23	3	3	CD
cord-014597-66vd2mdu	664	24	-	-	SYM
cord-014597-66vd2mdu	664	25	7	7	CD
cord-014597-66vd2mdu	664	26	of	of	IN
cord-014597-66vd2mdu	664	27	the	the	DT
cord-014597-66vd2mdu	664	28	cultures	culture	NNS
cord-014597-66vd2mdu	664	29	.	.	.
cord-014597-66vd2mdu	665	1	Viable	viable	JJ
cord-014597-66vd2mdu	665	2	cell	cell	NN
cord-014597-66vd2mdu	665	3	density	density	NN
cord-014597-66vd2mdu	665	4	and	and	CC
cord-014597-66vd2mdu	665	5	cell	cell	NN
cord-014597-66vd2mdu	665	6	viability	viability	NN
cord-014597-66vd2mdu	665	7	were	be	VBD
cord-014597-66vd2mdu	665	8	assessed	assess	VBN
cord-014597-66vd2mdu	665	9	by	by	IN
cord-014597-66vd2mdu	665	10	a	a	DT
cord-014597-66vd2mdu	665	11	Beckman	Beckman	NNP
cord-014597-66vd2mdu	665	12	Coulter	Coulter	NNP
cord-014597-66vd2mdu	665	13	Vi	Vi	NNP
cord-014597-66vd2mdu	665	14	-	-	HYPH
cord-014597-66vd2mdu	665	15	Cell	Cell	NNP
cord-014597-66vd2mdu	665	16	XR	XR	NNP
cord-014597-66vd2mdu	665	17	,	,	,
cord-014597-66vd2mdu	665	18	Ab	Ab	NNP
cord-014597-66vd2mdu	665	19	titer	titer	NN
cord-014597-66vd2mdu	665	20	was	be	VBD
cord-014597-66vd2mdu	665	21	assessed	assess	VBN
cord-014597-66vd2mdu	665	22	by	by	IN
cord-014597-66vd2mdu	665	23	a	a	DT
cord-014597-66vd2mdu	665	24	Pall	Pall	NNP
cord-014597-66vd2mdu	665	25	FortéBio	FortéBio	NNP
cord-014597-66vd2mdu	665	26	QK	QK	NNP
cord-014597-66vd2mdu	665	27	e	e	NN
cord-014597-66vd2mdu	665	28	,	,	,
cord-014597-66vd2mdu	665	29	and	and	CC
cord-014597-66vd2mdu	665	30	glycan	glycan	NN
cord-014597-66vd2mdu	665	31	analysis	analysis	NN
cord-014597-66vd2mdu	665	32	was	be	VBD
cord-014597-66vd2mdu	665	33	assessed	assess	VBN
cord-014597-66vd2mdu	665	34	by	by	IN
cord-014597-66vd2mdu	665	35	a	a	DT
cord-014597-66vd2mdu	665	36	PerkinElmer	PerkinElmer	NNP
cord-014597-66vd2mdu	665	37	LabChip	LabChip	NNP
cord-014597-66vd2mdu	665	38	GXII	GXII	NNP
cord-014597-66vd2mdu	665	39	.	.	.
cord-014597-66vd2mdu	666	1	For	for	IN
cord-014597-66vd2mdu	666	2	the	the	DT
cord-014597-66vd2mdu	666	3	functional	functional	JJ
cord-014597-66vd2mdu	666	4	CDC	CDC	NNP
cord-014597-66vd2mdu	666	5	assay	assay	NN
cord-014597-66vd2mdu	666	6	,	,	,
cord-014597-66vd2mdu	666	7	Abs	Abs	NNP
cord-014597-66vd2mdu	666	8	were	be	VBD
cord-014597-66vd2mdu	666	9	incubated	incubate	VBN
cord-014597-66vd2mdu	666	10	with	with	IN
cord-014597-66vd2mdu	666	11	Daudi	Daudi	NNP
cord-014597-66vd2mdu	666	12	B	B	NNP
cord-014597-66vd2mdu	666	13	lymphoblast	lymphoblast	NN
cord-014597-66vd2mdu	666	14	cells	cell	NNS
cord-014597-66vd2mdu	666	15	and	and	CC
cord-014597-66vd2mdu	666	16	normal	normal	JJ
cord-014597-66vd2mdu	666	17	human	human	JJ
cord-014597-66vd2mdu	666	18	complement	complement	NN
cord-014597-66vd2mdu	666	19	serum	serum	NN
cord-014597-66vd2mdu	666	20	.	.	.
cord-014597-66vd2mdu	667	1	Cell	cell	NN
cord-014597-66vd2mdu	667	2	cytotoxicity	cytotoxicity	NN
cord-014597-66vd2mdu	667	3	was	be	VBD
cord-014597-66vd2mdu	667	4	assessed	assess	VBN
cord-014597-66vd2mdu	667	5	with	with	IN
cord-014597-66vd2mdu	667	6	a	a	DT
cord-014597-66vd2mdu	667	7	Promega	Promega	NNP
cord-014597-66vd2mdu	667	8	CytoTox	CytoTox	NNP
cord-014597-66vd2mdu	667	9	-	-	HYPH
cord-014597-66vd2mdu	667	10	Glo	Glo	NNP
cord-014597-66vd2mdu	667	11	kit	kit	NN
cord-014597-66vd2mdu	667	12	.	.	.
cord-014597-66vd2mdu	668	1	Various	various	JJ
cord-014597-66vd2mdu	668	2	supplements	supplement	NNS
cord-014597-66vd2mdu	668	3	were	be	VBD
cord-014597-66vd2mdu	668	4	evaluated	evaluate	VBN
cord-014597-66vd2mdu	668	5	in	in	IN
cord-014597-66vd2mdu	668	6	fed	fed	JJ
cord-014597-66vd2mdu	668	7	-	-	HYPH
cord-014597-66vd2mdu	668	8	batch	batch	NN
cord-014597-66vd2mdu	668	9	cultures	culture	NNS
cord-014597-66vd2mdu	668	10	and	and	CC
cord-014597-66vd2mdu	668	11	resulted	result	VBD
cord-014597-66vd2mdu	668	12	in	in	IN
cord-014597-66vd2mdu	668	13	15	15	CD
cord-014597-66vd2mdu	668	14	-	-	SYM
cord-014597-66vd2mdu	668	15	45	45	CD
cord-014597-66vd2mdu	668	16	%	%	NN
cord-014597-66vd2mdu	668	17	Ab	ab	NN
cord-014597-66vd2mdu	668	18	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	668	19	without	without	IN
cord-014597-66vd2mdu	668	20	compromising	compromise	VBG
cord-014597-66vd2mdu	668	21	cell	cell	NN
cord-014597-66vd2mdu	668	22	growth	growth	NN
cord-014597-66vd2mdu	668	23	and	and	CC
cord-014597-66vd2mdu	668	24	Ab	Ab	NNP
cord-014597-66vd2mdu	668	25	titers	titer	NNS
cord-014597-66vd2mdu	668	26	.	.	.
cord-014597-66vd2mdu	669	1	Design	design	NN
cord-014597-66vd2mdu	669	2	of	of	IN
cord-014597-66vd2mdu	669	3	experiment	experiment	NN
cord-014597-66vd2mdu	669	4	analysis	analysis	NN
cord-014597-66vd2mdu	669	5	determined	determine	VBD
cord-014597-66vd2mdu	669	6	an	an	DT
cord-014597-66vd2mdu	669	7	optimal	optimal	JJ
cord-014597-66vd2mdu	669	8	composition	composition	NN
cord-014597-66vd2mdu	669	9	,	,	,
cord-014597-66vd2mdu	669	10	deemed	deem	VBN
cord-014597-66vd2mdu	669	11	"	"	``
cord-014597-66vd2mdu	669	12	Optimized	optimize	VBN
cord-014597-66vd2mdu	669	13	Supplement	Supplement	NNP
cord-014597-66vd2mdu	669	14	,	,	,
cord-014597-66vd2mdu	669	15	"	"	''
cord-014597-66vd2mdu	669	16	which	which	WDT
cord-014597-66vd2mdu	669	17	was	be	VBD
cord-014597-66vd2mdu	669	18	evaluated	evaluate	VBN
cord-014597-66vd2mdu	669	19	against	against	IN
cord-014597-66vd2mdu	669	20	a	a	DT
cord-014597-66vd2mdu	669	21	panel	panel	NN
cord-014597-66vd2mdu	669	22	of	of	IN
cord-014597-66vd2mdu	669	23	galactose	galactose	NN
cord-014597-66vd2mdu	669	24	-	-	HYPH
cord-014597-66vd2mdu	669	25	modulating	modulate	VBG
cord-014597-66vd2mdu	669	26	supplements	supplement	NNS
cord-014597-66vd2mdu	669	27	from	from	IN
cord-014597-66vd2mdu	669	28	other	other	JJ
cord-014597-66vd2mdu	669	29	suppliers	supplier	NNS
cord-014597-66vd2mdu	669	30	.	.	.
cord-014597-66vd2mdu	670	1	The	the	DT
cord-014597-66vd2mdu	670	2	Optimized	Optimized	NNP
cord-014597-66vd2mdu	670	3	Supplement	Supplement	NNP
cord-014597-66vd2mdu	670	4	resulted	result	VBD
cord-014597-66vd2mdu	670	5	in	in	IN
cord-014597-66vd2mdu	670	6	a	a	DT
cord-014597-66vd2mdu	670	7	similar	similar	JJ
cord-014597-66vd2mdu	670	8	viable	viable	JJ
cord-014597-66vd2mdu	670	9	cell	cell	NN
cord-014597-66vd2mdu	670	10	density	density	NN
cord-014597-66vd2mdu	670	11	(	(	-LRB-
cord-014597-66vd2mdu	670	12	VCD	VCD	NNP
cord-014597-66vd2mdu	670	13	)	)	-RRB-
cord-014597-66vd2mdu	670	14	and	and	CC
cord-014597-66vd2mdu	670	15	cell	cell	NN
cord-014597-66vd2mdu	670	16	viability	viability	NN
cord-014597-66vd2mdu	670	17	compared	compare	VBN
cord-014597-66vd2mdu	670	18	to	to	IN
cord-014597-66vd2mdu	670	19	the	the	DT
cord-014597-66vd2mdu	670	20	fed	feed	VBN
cord-014597-66vd2mdu	670	21	-	-	HYPH
cord-014597-66vd2mdu	670	22	batch	batch	NN
cord-014597-66vd2mdu	670	23	culture	culture	NN
cord-014597-66vd2mdu	670	24	Control	Control	NNP
cord-014597-66vd2mdu	670	25	which	which	WDT
cord-014597-66vd2mdu	670	26	had	have	VBD
cord-014597-66vd2mdu	670	27	no	no	DT
cord-014597-66vd2mdu	670	28	supplements	supplement	NNS
cord-014597-66vd2mdu	670	29	(	(	-LRB-
cord-014597-66vd2mdu	670	30	Fig	fig	NN
cord-014597-66vd2mdu	670	31	.	.	.
cord-014597-66vd2mdu	670	32	1a	1a	CD
cord-014597-66vd2mdu	670	33	)	)	-RRB-
cord-014597-66vd2mdu	670	34	.	.	.
cord-014597-66vd2mdu	671	1	Supplements	supplement	NNS
cord-014597-66vd2mdu	671	2	from	from	IN
cord-014597-66vd2mdu	671	3	Supplier	Supplier	NNP
cord-014597-66vd2mdu	671	4	1	1	CD
cord-014597-66vd2mdu	671	5	resulted	result	VBD
cord-014597-66vd2mdu	671	6	in	in	IN
cord-014597-66vd2mdu	671	7	similar	similar	JJ
cord-014597-66vd2mdu	671	8	to	to	IN
cord-014597-66vd2mdu	671	9	half	half	PDT
cord-014597-66vd2mdu	671	10	the	the	DT
cord-014597-66vd2mdu	671	11	VCD	vcd	NN
cord-014597-66vd2mdu	671	12	of	of	IN
cord-014597-66vd2mdu	671	13	the	the	DT
cord-014597-66vd2mdu	671	14	Control	Control	NNP
cord-014597-66vd2mdu	671	15	while	while	IN
cord-014597-66vd2mdu	671	16	supplements	supplement	NNS
cord-014597-66vd2mdu	671	17	from	from	IN
cord-014597-66vd2mdu	671	18	Supplier	Supplier	NNP
cord-014597-66vd2mdu	671	19	2	2	CD
cord-014597-66vd2mdu	671	20	resulted	result	VBD
cord-014597-66vd2mdu	671	21	in	in	IN
cord-014597-66vd2mdu	671	22	very	very	RB
cord-014597-66vd2mdu	671	23	low	low	JJ
cord-014597-66vd2mdu	671	24	VCD	vcd	NN
cord-014597-66vd2mdu	671	25	and	and	CC
cord-014597-66vd2mdu	671	26	percent	percent	NN
cord-014597-66vd2mdu	671	27	viability	viability	NN
cord-014597-66vd2mdu	671	28	.	.	.
cord-014597-66vd2mdu	672	1	All	all	DT
cord-014597-66vd2mdu	672	2	of	of	IN
cord-014597-66vd2mdu	672	3	the	the	DT
cord-014597-66vd2mdu	672	4	supplements	supplement	NNS
cord-014597-66vd2mdu	672	5	except	except	IN
cord-014597-66vd2mdu	672	6	those	those	DT
cord-014597-66vd2mdu	672	7	from	from	IN
cord-014597-66vd2mdu	672	8	Supplier	Supplier	NNP
cord-014597-66vd2mdu	672	9	2	2	CD
cord-014597-66vd2mdu	672	10	resulted	result	VBD
cord-014597-66vd2mdu	672	11	in	in	IN
cord-014597-66vd2mdu	672	12	Ab	Ab	NNP
cord-014597-66vd2mdu	672	13	titers	titer	NNS
cord-014597-66vd2mdu	672	14	similar	similar	JJ
cord-014597-66vd2mdu	672	15	to	to	IN
cord-014597-66vd2mdu	672	16	the	the	DT
cord-014597-66vd2mdu	672	17	Control	Control	NNP
cord-014597-66vd2mdu	673	1	(	(	-LRB-
cord-014597-66vd2mdu	673	2	Fig	Fig	NNP
cord-014597-66vd2mdu	673	3	.	.	.
cord-014597-66vd2mdu	673	4	1b	1b	CD
cord-014597-66vd2mdu	673	5	)	)	-RRB-
cord-014597-66vd2mdu	673	6	.	.	.
cord-014597-66vd2mdu	674	1	Due	due	IN
cord-014597-66vd2mdu	674	2	to	to	IN
cord-014597-66vd2mdu	674	3	the	the	DT
cord-014597-66vd2mdu	674	4	poor	poor	JJ
cord-014597-66vd2mdu	674	5	growth	growth	NN
cord-014597-66vd2mdu	674	6	and	and	CC
cord-014597-66vd2mdu	674	7	subsequently	subsequently	RB
cord-014597-66vd2mdu	674	8	low	low	JJ
cord-014597-66vd2mdu	674	9	titer	titer	NN
cord-014597-66vd2mdu	674	10	from	from	IN
cord-014597-66vd2mdu	674	11	Supplier	Supplier	NNP
cord-014597-66vd2mdu	674	12	2	2	CD
cord-014597-66vd2mdu	674	13	's	's	POS
cord-014597-66vd2mdu	674	14	supplement	supplement	NN
cord-014597-66vd2mdu	674	15	,	,	,
cord-014597-66vd2mdu	674	16	Supplier	Supplier	NNP
cord-014597-66vd2mdu	674	17	2	2	CD
cord-014597-66vd2mdu	674	18	was	be	VBD
cord-014597-66vd2mdu	674	19	not	not	RB
cord-014597-66vd2mdu	674	20	further	further	RB
cord-014597-66vd2mdu	674	21	evaluated	evaluate	VBN
cord-014597-66vd2mdu	674	22	.	.	.
cord-014597-66vd2mdu	675	1	The	the	DT
cord-014597-66vd2mdu	675	2	glycan	glycan	NN
cord-014597-66vd2mdu	675	3	profiles	profile	NNS
cord-014597-66vd2mdu	675	4	were	be	VBD
cord-014597-66vd2mdu	675	5	analyzed	analyze	VBN
cord-014597-66vd2mdu	675	6	and	and	CC
cord-014597-66vd2mdu	675	7	are	be	VBP
cord-014597-66vd2mdu	675	8	presented	present	VBN
cord-014597-66vd2mdu	675	9	in	in	IN
cord-014597-66vd2mdu	675	10	(	(	-LRB-
cord-014597-66vd2mdu	675	11	Fig	Fig	NNP
cord-014597-66vd2mdu	675	12	.	.	.
cord-014597-66vd2mdu	675	13	1c	1c	CD
cord-014597-66vd2mdu	675	14	)	)	-RRB-
cord-014597-66vd2mdu	675	15	.	.	.
cord-014597-66vd2mdu	676	1	All	all	PDT
cord-014597-66vd2mdu	676	2	the	the	DT
cord-014597-66vd2mdu	676	3	evaluated	evaluate	VBN
cord-014597-66vd2mdu	676	4	supplements	supplement	NNS
cord-014597-66vd2mdu	676	5	were	be	VBD
cord-014597-66vd2mdu	676	6	able	able	JJ
cord-014597-66vd2mdu	676	7	to	to	TO
cord-014597-66vd2mdu	676	8	raise	raise	VB
cord-014597-66vd2mdu	676	9	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	676	10	;	;	:
cord-014597-66vd2mdu	676	11	however	however	RB
cord-014597-66vd2mdu	676	12	,	,	,
cord-014597-66vd2mdu	676	13	only	only	RB
cord-014597-66vd2mdu	676	14	the	the	DT
cord-014597-66vd2mdu	676	15	Optimized	Optimized	NNP
cord-014597-66vd2mdu	676	16	Supplement	Supplement	NNP
cord-014597-66vd2mdu	676	17	and	and	CC
cord-014597-66vd2mdu	676	18	the	the	DT
cord-014597-66vd2mdu	676	19	2X	2x	CD
cord-014597-66vd2mdu	676	20	Supplier	Supplier	NNP
cord-014597-66vd2mdu	676	21	1	1	CD
cord-014597-66vd2mdu	676	22	Supplement	Supplement	NNP
cord-014597-66vd2mdu	676	23	resulted	result	VBD
cord-014597-66vd2mdu	676	24	in	in	IN
cord-014597-66vd2mdu	676	25	over	over	IN
cord-014597-66vd2mdu	676	26	40	40	CD
cord-014597-66vd2mdu	676	27	%	%	NN
cord-014597-66vd2mdu	676	28	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	676	29	.	.	.
cord-014597-66vd2mdu	677	1	The	the	DT
cord-014597-66vd2mdu	677	2	function	function	NN
cord-014597-66vd2mdu	677	3	of	of	IN
cord-014597-66vd2mdu	677	4	the	the	DT
cord-014597-66vd2mdu	677	5	Abs	Abs	NNP
cord-014597-66vd2mdu	677	6	was	be	VBD
cord-014597-66vd2mdu	677	7	further	further	RB
cord-014597-66vd2mdu	677	8	evaluated	evaluate	VBN
cord-014597-66vd2mdu	677	9	in	in	IN
cord-014597-66vd2mdu	677	10	a	a	DT
cord-014597-66vd2mdu	677	11	CDC	CDC	NNP
cord-014597-66vd2mdu	677	12	assay	assay	NN
cord-014597-66vd2mdu	677	13	(	(	-LRB-
cord-014597-66vd2mdu	677	14	Fig	Fig	NNP
cord-014597-66vd2mdu	677	15	.	.	.
cord-014597-66vd2mdu	677	16	1d	1d	CD
cord-014597-66vd2mdu	677	17	)	)	-RRB-
cord-014597-66vd2mdu	677	18	.	.	.
cord-014597-66vd2mdu	678	1	Abs	abs	NN
cord-014597-66vd2mdu	678	2	from	from	IN
cord-014597-66vd2mdu	678	3	the	the	DT
cord-014597-66vd2mdu	678	4	Optimized	Optimized	NNP
cord-014597-66vd2mdu	678	5	Supplement	Supplement	NNP
cord-014597-66vd2mdu	678	6	were	be	VBD
cord-014597-66vd2mdu	678	7	more	more	RBR
cord-014597-66vd2mdu	678	8	effective	effective	JJ
cord-014597-66vd2mdu	678	9	than	than	IN
cord-014597-66vd2mdu	678	10	the	the	DT
cord-014597-66vd2mdu	678	11	Control	Control	NNP
cord-014597-66vd2mdu	678	12	Abs	Abs	NNP
cord-014597-66vd2mdu	678	13	and	and	CC
cord-014597-66vd2mdu	678	14	had	have	VBD
cord-014597-66vd2mdu	678	15	a	a	DT
cord-014597-66vd2mdu	678	16	significantly	significantly	RB
cord-014597-66vd2mdu	678	17	lower	low	JJR
cord-014597-66vd2mdu	678	18	half	half	RB
cord-014597-66vd2mdu	678	19	-	-	HYPH
cord-014597-66vd2mdu	678	20	maximal	maximal	JJ
cord-014597-66vd2mdu	678	21	effective	effective	JJ
cord-014597-66vd2mdu	678	22	concentration	concentration	NN
cord-014597-66vd2mdu	678	23	(	(	-LRB-
cord-014597-66vd2mdu	678	24	EC	EC	NNP
cord-014597-66vd2mdu	678	25	50	50	CD
cord-014597-66vd2mdu	678	26	,	,	,
cord-014597-66vd2mdu	678	27	1.19	1.19	CD
cord-014597-66vd2mdu	678	28	μg	μg	NN
cord-014597-66vd2mdu	678	29	/	/	SYM
cord-014597-66vd2mdu	678	30	mL	mL	NNP
cord-014597-66vd2mdu	678	31	)	)	-RRB-
cord-014597-66vd2mdu	678	32	than	than	IN
cord-014597-66vd2mdu	678	33	the	the	DT
cord-014597-66vd2mdu	678	34	Control	Control	NNP
cord-014597-66vd2mdu	678	35	(	(	-LRB-
cord-014597-66vd2mdu	678	36	1.71	1.71	CD
cord-014597-66vd2mdu	678	37	μg	μg	NNP
cord-014597-66vd2mdu	678	38	/	/	SYM
cord-014597-66vd2mdu	678	39	mL	mL	NNP
cord-014597-66vd2mdu	678	40	)	)	-RRB-
cord-014597-66vd2mdu	678	41	.	.	.
cord-014597-66vd2mdu	679	1	Abs	abs	NN
cord-014597-66vd2mdu	679	2	from	from	IN
cord-014597-66vd2mdu	679	3	the	the	DT
cord-014597-66vd2mdu	679	4	2X	2x	CD
cord-014597-66vd2mdu	679	5	Supplier	Supplier	NNP
cord-014597-66vd2mdu	679	6	1	1	CD
cord-014597-66vd2mdu	679	7	Supplement	Supplement	NNP
cord-014597-66vd2mdu	679	8	had	have	VBD
cord-014597-66vd2mdu	679	9	a	a	DT
cord-014597-66vd2mdu	679	10	similar	similar	JJ
cord-014597-66vd2mdu	679	11	EC	EC	NNP
cord-014597-66vd2mdu	679	12	50	50	CD
cord-014597-66vd2mdu	679	13	to	to	IN
cord-014597-66vd2mdu	679	14	the	the	DT
cord-014597-66vd2mdu	679	15	Control	Control	NNP
cord-014597-66vd2mdu	679	16	which	which	WDT
cord-014597-66vd2mdu	679	17	may	may	MD
cord-014597-66vd2mdu	679	18	be	be	VB
cord-014597-66vd2mdu	679	19	due	due	JJ
cord-014597-66vd2mdu	679	20	to	to	IN
cord-014597-66vd2mdu	679	21	the	the	DT
cord-014597-66vd2mdu	679	22	higher	high	JJR
cord-014597-66vd2mdu	679	23	Man5	Man5	NNP
cord-014597-66vd2mdu	679	24	%	%	NN
cord-014597-66vd2mdu	679	25	of	of	IN
cord-014597-66vd2mdu	679	26	the	the	DT
cord-014597-66vd2mdu	679	27	Abs	Abs	NNP
cord-014597-66vd2mdu	679	28	.	.	.
cord-014597-66vd2mdu	680	1	An	an	DT
cord-014597-66vd2mdu	680	2	Optimized	optimize	VBN
cord-014597-66vd2mdu	680	3	Supplement	Supplement	NNP
cord-014597-66vd2mdu	680	4	was	be	VBD
cord-014597-66vd2mdu	680	5	produced	produce	VBN
cord-014597-66vd2mdu	680	6	through	through	IN
cord-014597-66vd2mdu	680	7	fed	fed	JJ
cord-014597-66vd2mdu	680	8	-	-	HYPH
cord-014597-66vd2mdu	680	9	batch	batch	NN
cord-014597-66vd2mdu	680	10	evaluation	evaluation	NN
cord-014597-66vd2mdu	680	11	and	and	CC
cord-014597-66vd2mdu	680	12	design	design	NN
cord-014597-66vd2mdu	680	13	of	of	IN
cord-014597-66vd2mdu	680	14	experiment	experiment	NN
cord-014597-66vd2mdu	680	15	analysis	analysis	NN
cord-014597-66vd2mdu	680	16	.	.	.
cord-014597-66vd2mdu	681	1	The	the	DT
cord-014597-66vd2mdu	681	2	Optimized	Optimized	NNP
cord-014597-66vd2mdu	681	3	Supplement	Supplement	NNP
cord-014597-66vd2mdu	681	4	outperformed	outperform	VBD
cord-014597-66vd2mdu	681	5	all	all	DT
cord-014597-66vd2mdu	681	6	other	other	JJ
cord-014597-66vd2mdu	681	7	supplenments	supplenment	NNS
cord-014597-66vd2mdu	681	8	from	from	IN
cord-014597-66vd2mdu	681	9	other	other	JJ
cord-014597-66vd2mdu	681	10	suppliers	supplier	NNS
cord-014597-66vd2mdu	681	11	and	and	CC
cord-014597-66vd2mdu	681	12	resulted	result	VBD
cord-014597-66vd2mdu	681	13	in	in	IN
cord-014597-66vd2mdu	681	14	the	the	DT
cord-014597-66vd2mdu	681	15	best	good	JJS
cord-014597-66vd2mdu	681	16	overall	overall	JJ
cord-014597-66vd2mdu	681	17	cell	cell	NN
cord-014597-66vd2mdu	681	18	growth	growth	NN
cord-014597-66vd2mdu	681	19	,	,	,
cord-014597-66vd2mdu	681	20	glycan	glycan	NN
cord-014597-66vd2mdu	681	21	profile	profile	NN
cord-014597-66vd2mdu	681	22	,	,	,
cord-014597-66vd2mdu	681	23	and	and	CC
cord-014597-66vd2mdu	681	24	functional	functional	JJ
cord-014597-66vd2mdu	681	25	Ab	ab	NN
cord-014597-66vd2mdu	681	26	activity	activity	NN
cord-014597-66vd2mdu	681	27	(	(	-LRB-
cord-014597-66vd2mdu	681	28	Table	table	NN
cord-014597-66vd2mdu	681	29	1	1	CD
cord-014597-66vd2mdu	681	30	)	)	-RRB-
cord-014597-66vd2mdu	681	31	.	.	.
cord-014597-66vd2mdu	682	1	Industry	industry	NN
cord-014597-66vd2mdu	682	2	practice	practice	NN
cord-014597-66vd2mdu	682	3	for	for	IN
cord-014597-66vd2mdu	682	4	mammalian	mammalian	JJ
cord-014597-66vd2mdu	682	5	cell	cell	NN
cord-014597-66vd2mdu	682	6	culture	culture	NN
cord-014597-66vd2mdu	682	7	media	medium	NNS
cord-014597-66vd2mdu	682	8	and	and	CC
cord-014597-66vd2mdu	682	9	feed	feed	NN
cord-014597-66vd2mdu	682	10	development	development	NN
cord-014597-66vd2mdu	682	11	typically	typically	RB
cord-014597-66vd2mdu	682	12	employs	employ	VBZ
cord-014597-66vd2mdu	682	13	a	a	DT
cord-014597-66vd2mdu	682	14	high	high	JJ
cord-014597-66vd2mdu	682	15	-	-	HYPH
cord-014597-66vd2mdu	682	16	throughput	throughput	NN
cord-014597-66vd2mdu	682	17	screening	screening	NN
cord-014597-66vd2mdu	682	18	(	(	-LRB-
cord-014597-66vd2mdu	682	19	HTS	HTS	NNP
cord-014597-66vd2mdu	682	20	)	)	-RRB-
cord-014597-66vd2mdu	682	21	platform	platform	NN
cord-014597-66vd2mdu	682	22	along	along	IN
cord-014597-66vd2mdu	682	23	with	with	IN
cord-014597-66vd2mdu	682	24	large	large	JJ
cord-014597-66vd2mdu	682	25	sets	set	NNS
cord-014597-66vd2mdu	682	26	of	of	IN
cord-014597-66vd2mdu	682	27	experiments	experiment	NNS
cord-014597-66vd2mdu	682	28	[	[	-LRB-
cord-014597-66vd2mdu	682	29	1	1	CD
cord-014597-66vd2mdu	682	30	]	]	-RRB-
cord-014597-66vd2mdu	682	31	.	.	.
cord-014597-66vd2mdu	683	1	Modern	modern	JJ
cord-014597-66vd2mdu	683	2	HTS	HTS	NNP
cord-014597-66vd2mdu	683	3	systems	system	NNS
cord-014597-66vd2mdu	683	4	often	often	RB
cord-014597-66vd2mdu	683	5	include	include	VBP
cord-014597-66vd2mdu	683	6	robotic	robotic	JJ
cord-014597-66vd2mdu	683	7	liquid	liquid	JJ
cord-014597-66vd2mdu	683	8	handlers	handler	NNS
cord-014597-66vd2mdu	683	9	to	to	TO
cord-014597-66vd2mdu	683	10	replace	replace	VB
cord-014597-66vd2mdu	683	11	labor	labor	NN
cord-014597-66vd2mdu	683	12	intensive	intensive	JJ
cord-014597-66vd2mdu	683	13	steps	step	NNS
cord-014597-66vd2mdu	683	14	.	.	.
cord-014597-66vd2mdu	684	1	To	to	TO
cord-014597-66vd2mdu	684	2	align	align	VB
cord-014597-66vd2mdu	684	3	with	with	IN
cord-014597-66vd2mdu	684	4	advancements	advancement	NNS
cord-014597-66vd2mdu	684	5	in	in	IN
cord-014597-66vd2mdu	684	6	the	the	DT
cord-014597-66vd2mdu	684	7	field	field	NN
cord-014597-66vd2mdu	684	8	,	,	,
cord-014597-66vd2mdu	684	9	a	a	DT
cord-014597-66vd2mdu	684	10	semi	semi	RB
cord-014597-66vd2mdu	684	11	-	-	JJ
cord-014597-66vd2mdu	684	12	automated	automated	JJ
cord-014597-66vd2mdu	684	13	HTS	HTS	NNP
cord-014597-66vd2mdu	684	14	platform	platform	NN
cord-014597-66vd2mdu	684	15	was	be	VBD
cord-014597-66vd2mdu	684	16	developed	develop	VBN
cord-014597-66vd2mdu	684	17	to	to	TO
cord-014597-66vd2mdu	684	18	facilitate	facilitate	VB
cord-014597-66vd2mdu	684	19	in	in	IN
cord-014597-66vd2mdu	684	20	-	-	HYPH
cord-014597-66vd2mdu	684	21	house	house	NN
cord-014597-66vd2mdu	684	22	media	medium	NNS
cord-014597-66vd2mdu	684	23	and	and	CC
cord-014597-66vd2mdu	684	24	feed	feed	VB
cord-014597-66vd2mdu	684	25	development	development	NN
cord-014597-66vd2mdu	684	26	for	for	IN
cord-014597-66vd2mdu	684	27	early	early	JJ
cord-014597-66vd2mdu	684	28	stage	stage	NN
cord-014597-66vd2mdu	684	29	biologics	biologic	NNS
cord-014597-66vd2mdu	684	30	projects	project	NNS
cord-014597-66vd2mdu	684	31	.	.	.
cord-014597-66vd2mdu	685	1	Selecting	select	VBG
cord-014597-66vd2mdu	685	2	appropriate	appropriate	JJ
cord-014597-66vd2mdu	685	3	instruments	instrument	NNS
cord-014597-66vd2mdu	685	4	and	and	CC
cord-014597-66vd2mdu	685	5	integrating	integrate	VBG
cord-014597-66vd2mdu	685	6	them	-PRON-	PRP
cord-014597-66vd2mdu	685	7	into	into	IN
cord-014597-66vd2mdu	685	8	a	a	DT
cord-014597-66vd2mdu	685	9	seamless	seamless	JJ
cord-014597-66vd2mdu	685	10	system	system	NN
cord-014597-66vd2mdu	685	11	are	be	VBP
cord-014597-66vd2mdu	685	12	the	the	DT
cord-014597-66vd2mdu	685	13	keys	key	NNS
cord-014597-66vd2mdu	685	14	to	to	IN
cord-014597-66vd2mdu	685	15	a	a	DT
cord-014597-66vd2mdu	685	16	HTS	HTS	NNP
cord-014597-66vd2mdu	685	17	platform	platform	NN
cord-014597-66vd2mdu	685	18	.	.	.
cord-014597-66vd2mdu	686	1	The	the	DT
cord-014597-66vd2mdu	686	2	developed	developed	JJ
cord-014597-66vd2mdu	686	3	HTS	HTS	NNP
cord-014597-66vd2mdu	686	4	platform	platform	NN
cord-014597-66vd2mdu	686	5	uses	use	VBZ
cord-014597-66vd2mdu	686	6	24	24	CD
cord-014597-66vd2mdu	686	7	deep	deep	JJ
cord-014597-66vd2mdu	686	8	well	well	NN
cord-014597-66vd2mdu	686	9	plates	plate	NNS
cord-014597-66vd2mdu	686	10	(	(	-LRB-
cord-014597-66vd2mdu	686	11	DWPs	DWPs	NNP
cord-014597-66vd2mdu	686	12	)	)	-RRB-
cord-014597-66vd2mdu	686	13	for	for	IN
cord-014597-66vd2mdu	686	14	culture	culture	NN
cord-014597-66vd2mdu	686	15	vessels	vessel	NNS
cord-014597-66vd2mdu	686	16	,	,	,
cord-014597-66vd2mdu	686	17	the	the	DT
cord-014597-66vd2mdu	686	18	liquid	liquid	JJ
cord-014597-66vd2mdu	686	19	handler	handler	NN
cord-014597-66vd2mdu	686	20	of	of	IN
cord-014597-66vd2mdu	686	21	the	the	DT
cord-014597-66vd2mdu	686	22	Advance	Advance	NNP
cord-014597-66vd2mdu	686	23	Microscale	Microscale	NNP
cord-014597-66vd2mdu	686	24	BioReactor	BioReactor	NNP
cord-014597-66vd2mdu	686	25	(	(	-LRB-
cord-014597-66vd2mdu	686	26	AMBR15	ambr15	NN
cord-014597-66vd2mdu	686	27	)	)	-RRB-
cord-014597-66vd2mdu	686	28	for	for	IN
cord-014597-66vd2mdu	686	29	media/	media/	ADD
cord-014597-66vd2mdu	686	30	feed	feed	NN
cord-014597-66vd2mdu	686	31	formulation	formulation	NN
cord-014597-66vd2mdu	686	32	preparation	preparation	NN
cord-014597-66vd2mdu	686	33	in	in	IN
cord-014597-66vd2mdu	686	34	an	an	DT
cord-014597-66vd2mdu	686	35	aseptic	aseptic	JJ
cord-014597-66vd2mdu	686	36	environment	environment	NN
cord-014597-66vd2mdu	686	37	,	,	,
cord-014597-66vd2mdu	686	38	NyONE	NyONE	NNP
cord-014597-66vd2mdu	686	39	cell	cell	NN
cord-014597-66vd2mdu	686	40	imager	imager	NN
cord-014597-66vd2mdu	686	41	for	for	IN
cord-014597-66vd2mdu	686	42	viability	viability	NN
cord-014597-66vd2mdu	686	43	and	and	CC
cord-014597-66vd2mdu	686	44	cell	cell	NN
cord-014597-66vd2mdu	686	45	growth	growth	NN
cord-014597-66vd2mdu	686	46	analysis	analysis	NN
cord-014597-66vd2mdu	686	47	,	,	,
cord-014597-66vd2mdu	686	48	TECAN	TECAN	NNP
cord-014597-66vd2mdu	686	49	Freedom	Freedom	NNP
cord-014597-66vd2mdu	686	50	EVO	EVO	NNP
cord-014597-66vd2mdu	686	51	's	's	POS
cord-014597-66vd2mdu	686	52	liquid	liquid	JJ
cord-014597-66vd2mdu	686	53	handler	handler	NN
cord-014597-66vd2mdu	686	54	for	for	IN
cord-014597-66vd2mdu	686	55	activity	activity	NN
cord-014597-66vd2mdu	686	56	assay	assay	NN
cord-014597-66vd2mdu	686	57	sample	sample	NN
cord-014597-66vd2mdu	686	58	preparation	preparation	NN
cord-014597-66vd2mdu	686	59	,	,	,
cord-014597-66vd2mdu	686	60	and	and	CC
cord-014597-66vd2mdu	686	61	Cedex	Cedex	NNP
cord-014597-66vd2mdu	686	62	BioHT	BioHT	NNP
cord-014597-66vd2mdu	686	63	for	for	IN
cord-014597-66vd2mdu	686	64	high	high	JJ
cord-014597-66vd2mdu	686	65	-	-	HYPH
cord-014597-66vd2mdu	686	66	throughput	throughput	NN
cord-014597-66vd2mdu	686	67	metabolite	metabolite	NN
cord-014597-66vd2mdu	686	68	analysis	analysis	NN
cord-014597-66vd2mdu	686	69	.	.	.
cord-014597-66vd2mdu	687	1	24	24	CD
cord-014597-66vd2mdu	687	2	DWPs	dwp	NNS
cord-014597-66vd2mdu	687	3	offer	offer	VBP
cord-014597-66vd2mdu	687	4	comparable	comparable	JJ
cord-014597-66vd2mdu	687	5	cell	cell	NN
cord-014597-66vd2mdu	687	6	growth	growth	NN
cord-014597-66vd2mdu	687	7	to	to	TO
cord-014597-66vd2mdu	687	8	shake	shake	VB
cord-014597-66vd2mdu	687	9	flasks	flask	NNS
cord-014597-66vd2mdu	687	10	and	and	CC
cord-014597-66vd2mdu	687	11	compatible	compatible	JJ
cord-014597-66vd2mdu	687	12	layout	layout	NN
cord-014597-66vd2mdu	687	13	to	to	IN
cord-014597-66vd2mdu	687	14	AMBR15	AMBR15	NNP
cord-014597-66vd2mdu	687	15	,	,	,
cord-014597-66vd2mdu	687	16	which	which	WDT
cord-014597-66vd2mdu	687	17	makes	make	VBZ
cord-014597-66vd2mdu	687	18	the	the	DT
cord-014597-66vd2mdu	687	19	24	24	CD
cord-014597-66vd2mdu	687	20	DWP	DWP	NNP
cord-014597-66vd2mdu	687	21	an	an	DT
cord-014597-66vd2mdu	687	22	ideal	ideal	JJ
cord-014597-66vd2mdu	687	23	candidate	candidate	NN
cord-014597-66vd2mdu	687	24	for	for	IN
cord-014597-66vd2mdu	687	25	the	the	DT
cord-014597-66vd2mdu	687	26	platform	platform	NN
cord-014597-66vd2mdu	687	27	.	.	.
cord-014597-66vd2mdu	688	1	In	in	IN
cord-014597-66vd2mdu	688	2	addition	addition	NN
cord-014597-66vd2mdu	688	3	,	,	,
cord-014597-66vd2mdu	688	4	the	the	DT
cord-014597-66vd2mdu	688	5	user	user	NN
cord-014597-66vd2mdu	688	6	friendly	friendly	JJ
cord-014597-66vd2mdu	688	7	Design	design	NN
cord-014597-66vd2mdu	688	8	of	of	IN
cord-014597-66vd2mdu	688	9	Experiments	Experiments	NNP
cord-014597-66vd2mdu	688	10	(	(	-LRB-
cord-014597-66vd2mdu	688	11	DoEs	does	JJ
cord-014597-66vd2mdu	688	12	)	)	-RRB-
cord-014597-66vd2mdu	688	13	interface	interface	NN
cord-014597-66vd2mdu	688	14	and	and	CC
cord-014597-66vd2mdu	688	15	liquid	liquid	JJ
cord-014597-66vd2mdu	688	16	handler	handler	NN
cord-014597-66vd2mdu	688	17	function	function	NN
cord-014597-66vd2mdu	688	18	of	of	IN
cord-014597-66vd2mdu	688	19	the	the	DT
cord-014597-66vd2mdu	688	20	AMBR15	AMBR15	NNP
cord-014597-66vd2mdu	688	21	expediates	expediate	VBZ
cord-014597-66vd2mdu	688	22	the	the	DT
cord-014597-66vd2mdu	688	23	formulation	formulation	NN
cord-014597-66vd2mdu	688	24	preparation	preparation	NN
cord-014597-66vd2mdu	688	25	of	of	IN
cord-014597-66vd2mdu	688	26	varying	vary	VBG
cord-014597-66vd2mdu	688	27	DoE	doe	NN
cord-014597-66vd2mdu	688	28	conditions	condition	NNS
cord-014597-66vd2mdu	688	29	[	[	-LRB-
cord-014597-66vd2mdu	688	30	2	2	CD
cord-014597-66vd2mdu	688	31	]	]	-RRB-
cord-014597-66vd2mdu	688	32	.	.	.
cord-014597-66vd2mdu	689	1	A	a	DT
cord-014597-66vd2mdu	689	2	macro	macro	JJ
cord-014597-66vd2mdu	689	3	program	program	NN
cord-014597-66vd2mdu	689	4	was	be	VBD
cord-014597-66vd2mdu	689	5	written	write	VBN
cord-014597-66vd2mdu	689	6	and	and	CC
cord-014597-66vd2mdu	689	7	developed	develop	VBN
cord-014597-66vd2mdu	689	8	in	in	IN
cord-014597-66vd2mdu	689	9	Excel	Excel	NNP
cord-014597-66vd2mdu	689	10	to	to	TO
cord-014597-66vd2mdu	689	11	enable	enable	VB
cord-014597-66vd2mdu	689	12	the	the	DT
cord-014597-66vd2mdu	689	13	easy	easy	JJ
cord-014597-66vd2mdu	689	14	import	import	NN
cord-014597-66vd2mdu	689	15	of	of	IN
cord-014597-66vd2mdu	689	16	DoEs	DoEs	NNP
cord-014597-66vd2mdu	689	17	design	design	NN
cord-014597-66vd2mdu	689	18	from	from	IN
cord-014597-66vd2mdu	689	19	major	major	JJ
cord-014597-66vd2mdu	689	20	statistics	statistic	NNS
cord-014597-66vd2mdu	689	21	software	software	NN
cord-014597-66vd2mdu	689	22	packages	package	NNS
cord-014597-66vd2mdu	689	23	,	,	,
cord-014597-66vd2mdu	689	24	such	such	JJ
cord-014597-66vd2mdu	689	25	as	as	IN
cord-014597-66vd2mdu	689	26	JMP	JMP	NNP
cord-014597-66vd2mdu	689	27	and	and	CC
cord-014597-66vd2mdu	689	28	Simca	Simca	NNP
cord-014597-66vd2mdu	689	29	,	,	,
cord-014597-66vd2mdu	689	30	into	into	IN
cord-014597-66vd2mdu	689	31	AMBR15	ambr15	NN
cord-014597-66vd2mdu	689	32	.	.	.
cord-014597-66vd2mdu	690	1	Performance	performance	NN
cord-014597-66vd2mdu	690	2	qualification	qualification	NN
cord-014597-66vd2mdu	690	3	of	of	IN
cord-014597-66vd2mdu	690	4	each	each	DT
cord-014597-66vd2mdu	690	5	component	component	NN
cord-014597-66vd2mdu	690	6	were	be	VBD
cord-014597-66vd2mdu	690	7	performed	perform	VBN
cord-014597-66vd2mdu	690	8	prior	prior	RB
cord-014597-66vd2mdu	690	9	to	to	IN
cord-014597-66vd2mdu	690	10	implementing	implement	VBG
cord-014597-66vd2mdu	690	11	the	the	DT
cord-014597-66vd2mdu	690	12	HTS	HTS	NNP
cord-014597-66vd2mdu	690	13	platform	platform	NN
cord-014597-66vd2mdu	690	14	.	.	.
cord-014597-66vd2mdu	691	1	Comparable	comparable	JJ
cord-014597-66vd2mdu	691	2	cell	cell	NN
cord-014597-66vd2mdu	691	3	growth	growth	NN
cord-014597-66vd2mdu	691	4	profile	profile	NN
cord-014597-66vd2mdu	691	5	and	and	CC
cord-014597-66vd2mdu	691	6	productivity	productivity	NN
cord-014597-66vd2mdu	691	7	were	be	VBD
cord-014597-66vd2mdu	691	8	achived	achive	VBN
cord-014597-66vd2mdu	691	9	between	between	IN
cord-014597-66vd2mdu	691	10	shake	shake	NN
cord-014597-66vd2mdu	691	11	flasks	flask	NNS
cord-014597-66vd2mdu	691	12	and	and	CC
cord-014597-66vd2mdu	691	13	24	24	CD
cord-014597-66vd2mdu	691	14	DWPs	dwp	NNS
cord-014597-66vd2mdu	691	15	(	(	-LRB-
cord-014597-66vd2mdu	691	16	Fig	Fig	NNP
cord-014597-66vd2mdu	691	17	.	.	.
cord-014597-66vd2mdu	691	18	1a	1a	CD
cord-014597-66vd2mdu	691	19	)	)	-RRB-
cord-014597-66vd2mdu	691	20	,	,	,
cord-014597-66vd2mdu	691	21	indicating	indicate	VBG
cord-014597-66vd2mdu	691	22	compatable	compatable	JJ
cord-014597-66vd2mdu	691	23	cell	cell	NN
cord-014597-66vd2mdu	691	24	culture	culture	NN
cord-014597-66vd2mdu	691	25	environment	environment	NN
cord-014597-66vd2mdu	691	26	for	for	IN
cord-014597-66vd2mdu	691	27	the	the	DT
cord-014597-66vd2mdu	691	28	cells	cell	NNS
cord-014597-66vd2mdu	691	29	.	.	.
cord-014597-66vd2mdu	692	1	Cell	cell	NN
cord-014597-66vd2mdu	692	2	counts	count	VBZ
cord-014597-66vd2mdu	692	3	using	use	VBG
cord-014597-66vd2mdu	692	4	NyONE	NyONE	NNP
cord-014597-66vd2mdu	692	5	gave	give	VBD
cord-014597-66vd2mdu	692	6	identical	identical	JJ
cord-014597-66vd2mdu	692	7	cell	cell	NN
cord-014597-66vd2mdu	692	8	growth	growth	NN
cord-014597-66vd2mdu	692	9	ranking	rank	VBG
cord-014597-66vd2mdu	692	10	as	as	IN
cord-014597-66vd2mdu	692	11	the	the	DT
cord-014597-66vd2mdu	692	12	traditional	traditional	JJ
cord-014597-66vd2mdu	692	13	count	count	NN
cord-014597-66vd2mdu	692	14	from	from	IN
cord-014597-66vd2mdu	692	15	Vi	Vi	NNP
cord-014597-66vd2mdu	692	16	-	-	HYPH
cord-014597-66vd2mdu	692	17	Cell	Cell	NNP
cord-014597-66vd2mdu	692	18	XR	XR	NNP
cord-014597-66vd2mdu	692	19	(	(	-LRB-
cord-014597-66vd2mdu	692	20	Fig	fig	NN
cord-014597-66vd2mdu	692	21	.	.	.
cord-014597-66vd2mdu	692	22	1b	1b	CD
cord-014597-66vd2mdu	692	23	)	)	-RRB-
cord-014597-66vd2mdu	692	24	.	.	.
cord-014597-66vd2mdu	693	1	Freedom	Freedom	NNP
cord-014597-66vd2mdu	693	2	EVO	EVO	NNP
cord-014597-66vd2mdu	693	3	's	's	POS
cord-014597-66vd2mdu	693	4	liquid	liquid	JJ
cord-014597-66vd2mdu	693	5	handler	handler	NN
cord-014597-66vd2mdu	693	6	was	be	VBD
cord-014597-66vd2mdu	693	7	optimized	optimize	VBN
cord-014597-66vd2mdu	693	8	to	to	TO
cord-014597-66vd2mdu	693	9	produce	produce	VB
cord-014597-66vd2mdu	693	10	comparable	comparable	JJ
cord-014597-66vd2mdu	693	11	activity	activity	NN
cord-014597-66vd2mdu	693	12	results	result	NNS
cord-014597-66vd2mdu	693	13	to	to	IN
cord-014597-66vd2mdu	693	14	manual	manual	JJ
cord-014597-66vd2mdu	693	15	operation	operation	NN
cord-014597-66vd2mdu	693	16	while	while	IN
cord-014597-66vd2mdu	693	17	expediting	expedite	VBG
cord-014597-66vd2mdu	693	18	the	the	DT
cord-014597-66vd2mdu	693	19	sample	sample	NN
cord-014597-66vd2mdu	693	20	preparation	preparation	NN
cord-014597-66vd2mdu	693	21	with	with	IN
cord-014597-66vd2mdu	693	22	improved	improved	JJ
cord-014597-66vd2mdu	693	23	consistency	consistency	NN
cord-014597-66vd2mdu	693	24	(	(	-LRB-
cord-014597-66vd2mdu	693	25	Fig	Fig	NNP
cord-014597-66vd2mdu	693	26	.	.	.
cord-014597-66vd2mdu	693	27	1c	1c	CD
cord-014597-66vd2mdu	693	28	)	)	-RRB-
cord-014597-66vd2mdu	693	29	.	.	.
cord-014597-66vd2mdu	694	1	Finally	finally	RB
cord-014597-66vd2mdu	694	2	,	,	,
cord-014597-66vd2mdu	694	3	implementing	implement	VBG
cord-014597-66vd2mdu	694	4	the	the	DT
cord-014597-66vd2mdu	694	5	liquid	liquid	JJ
cord-014597-66vd2mdu	694	6	handler	handler	NN
cord-014597-66vd2mdu	694	7	function	function	NN
cord-014597-66vd2mdu	694	8	of	of	IN
cord-014597-66vd2mdu	694	9	AMBR15	ambr15	NN
cord-014597-66vd2mdu	694	10	to	to	TO
cord-014597-66vd2mdu	694	11	support	support	VB
cord-014597-66vd2mdu	694	12	media	medium	NNS
cord-014597-66vd2mdu	694	13	and	and	CC
cord-014597-66vd2mdu	694	14	feed	feed	NN
cord-014597-66vd2mdu	694	15	formulation	formulation	NN
cord-014597-66vd2mdu	694	16	significantly	significantly	RB
cord-014597-66vd2mdu	694	17	reduced	reduce	VBD
cord-014597-66vd2mdu	694	18	the	the	DT
cord-014597-66vd2mdu	694	19	labor	labor	NN
cord-014597-66vd2mdu	694	20	for	for	IN
cord-014597-66vd2mdu	694	21	each	each	DT
cord-014597-66vd2mdu	694	22	experiment	experiment	NN
cord-014597-66vd2mdu	694	23	.	.	.
cord-014597-66vd2mdu	695	1	Summary	summary	NN
cord-014597-66vd2mdu	695	2	of	of	IN
cord-014597-66vd2mdu	695	3	the	the	DT
cord-014597-66vd2mdu	695	4	capability	capability	NN
cord-014597-66vd2mdu	695	5	comparison	comparison	NN
cord-014597-66vd2mdu	695	6	between	between	IN
cord-014597-66vd2mdu	695	7	the	the	DT
cord-014597-66vd2mdu	695	8	HTS	HTS	NNP
cord-014597-66vd2mdu	695	9	platform	platform	NN
cord-014597-66vd2mdu	695	10	and	and	CC
cord-014597-66vd2mdu	695	11	the	the	DT
cord-014597-66vd2mdu	695	12	traditional	traditional	JJ
cord-014597-66vd2mdu	695	13	method	method	NN
cord-014597-66vd2mdu	695	14	are	be	VBP
cord-014597-66vd2mdu	695	15	listed	list	VBN
cord-014597-66vd2mdu	695	16	in	in	IN
cord-014597-66vd2mdu	695	17	Table	Table	NNP
cord-014597-66vd2mdu	695	18	1	1	CD
cord-014597-66vd2mdu	695	19	.	.	.
cord-014597-66vd2mdu	696	1	A	a	DT
cord-014597-66vd2mdu	696	2	case	case	NN
cord-014597-66vd2mdu	696	3	study	study	NN
cord-014597-66vd2mdu	696	4	of	of	IN
cord-014597-66vd2mdu	696	5	a	a	DT
cord-014597-66vd2mdu	696	6	complex	complex	JJ
cord-014597-66vd2mdu	696	7	feed	feed	NN
cord-014597-66vd2mdu	696	8	screening	screening	NN
cord-014597-66vd2mdu	696	9	with	with	IN
cord-014597-66vd2mdu	696	10	definitive	definitive	JJ
cord-014597-66vd2mdu	696	11	screening	screening	NN
cord-014597-66vd2mdu	696	12	design	design	NN
cord-014597-66vd2mdu	696	13	was	be	VBD
cord-014597-66vd2mdu	696	14	completed	complete	VBN
cord-014597-66vd2mdu	696	15	using	use	VBG
cord-014597-66vd2mdu	696	16	the	the	DT
cord-014597-66vd2mdu	696	17	semi	semi	JJ
cord-014597-66vd2mdu	696	18	-	-	JJ
cord-014597-66vd2mdu	696	19	automatic	automatic	JJ
cord-014597-66vd2mdu	696	20	HTS	HTS	NNP
cord-014597-66vd2mdu	696	21	platform	platform	NN
cord-014597-66vd2mdu	696	22	.	.	.
cord-014597-66vd2mdu	697	1	This	this	DT
cord-014597-66vd2mdu	697	2	experiment	experiment	NN
cord-014597-66vd2mdu	697	3	,	,	,
cord-014597-66vd2mdu	697	4	containing	contain	VBG
cord-014597-66vd2mdu	697	5	more	more	JJR
cord-014597-66vd2mdu	697	6	than	than	IN
cord-014597-66vd2mdu	697	7	60	60	CD
cord-014597-66vd2mdu	697	8	feed	feed	NN
cord-014597-66vd2mdu	697	9	formulations	formulation	NNS
cord-014597-66vd2mdu	697	10	in	in	IN
cord-014597-66vd2mdu	697	11	duplicates	duplicate	NNS
cord-014597-66vd2mdu	697	12	,	,	,
cord-014597-66vd2mdu	697	13	was	be	VBD
cord-014597-66vd2mdu	697	14	handled	handle	VBN
cord-014597-66vd2mdu	697	15	by	by	IN
cord-014597-66vd2mdu	697	16	one	one	CD
cord-014597-66vd2mdu	697	17	operator	operator	NN
cord-014597-66vd2mdu	697	18	and	and	CC
cord-014597-66vd2mdu	697	19	delivered	deliver	VBD
cord-014597-66vd2mdu	697	20	a	a	DT
cord-014597-66vd2mdu	697	21	40	40	CD
cord-014597-66vd2mdu	697	22	%	%	NN
cord-014597-66vd2mdu	697	23	improvement	improvement	NN
cord-014597-66vd2mdu	697	24	in	in	IN
cord-014597-66vd2mdu	697	25	productivity	productivity	NN
cord-014597-66vd2mdu	697	26	within	within	IN
cord-014597-66vd2mdu	697	27	a	a	DT
cord-014597-66vd2mdu	697	28	4	4	CD
cord-014597-66vd2mdu	697	29	week	week	NN
cord-014597-66vd2mdu	697	30	period	period	NN
cord-014597-66vd2mdu	697	31	(	(	-LRB-
cord-014597-66vd2mdu	697	32	Data	Data	NNP
cord-014597-66vd2mdu	697	33	not	not	RB
cord-014597-66vd2mdu	697	34	shown	show	VBN
cord-014597-66vd2mdu	697	35	)	)	-RRB-
cord-014597-66vd2mdu	697	36	.	.	.
cord-014597-66vd2mdu	698	1	In	in	IN
cord-014597-66vd2mdu	698	2	addition	addition	NN
cord-014597-66vd2mdu	698	3	,	,	,
cord-014597-66vd2mdu	698	4	implementing	implement	VBG
cord-014597-66vd2mdu	698	5	the	the	DT
cord-014597-66vd2mdu	698	6	HTS	HTS	NNP
cord-014597-66vd2mdu	698	7	platform	platform	NN
cord-014597-66vd2mdu	698	8	for	for	IN
cord-014597-66vd2mdu	698	9	this	this	DT
cord-014597-66vd2mdu	698	10	study	study	NN
cord-014597-66vd2mdu	698	11	also	also	RB
cord-014597-66vd2mdu	698	12	resulted	result	VBD
cord-014597-66vd2mdu	698	13	into~80	into~80	NNP
cord-014597-66vd2mdu	698	14	%	%	NN
cord-014597-66vd2mdu	698	15	reduction	reduction	NN
cord-014597-66vd2mdu	698	16	in	in	IN
cord-014597-66vd2mdu	698	17	labor	labor	NN
cord-014597-66vd2mdu	698	18	while	while	IN
cord-014597-66vd2mdu	698	19	improving	improve	VBG
cord-014597-66vd2mdu	698	20	the	the	DT
cord-014597-66vd2mdu	698	21	traceability	traceability	NN
cord-014597-66vd2mdu	698	22	of	of	IN
cord-014597-66vd2mdu	698	23	formulation	formulation	NN
cord-014597-66vd2mdu	698	24	preparation	preparation	NN
cord-014597-66vd2mdu	698	25	.	.	.
cord-014597-66vd2mdu	699	1	A	a	DT
cord-014597-66vd2mdu	699	2	semi	semi	RB
cord-014597-66vd2mdu	699	3	-	-	JJ
cord-014597-66vd2mdu	699	4	automated	automated	JJ
cord-014597-66vd2mdu	699	5	HTS	HTS	NNP
cord-014597-66vd2mdu	699	6	platform	platform	NN
cord-014597-66vd2mdu	699	7	was	be	VBD
cord-014597-66vd2mdu	699	8	developed	develop	VBN
cord-014597-66vd2mdu	699	9	to	to	TO
cord-014597-66vd2mdu	699	10	support	support	VB
cord-014597-66vd2mdu	699	11	media	medium	NNS
cord-014597-66vd2mdu	699	12	and	and	CC
cord-014597-66vd2mdu	699	13	feeds	feed	VBZ
cord-014597-66vd2mdu	699	14	screening	screening	NN
cord-014597-66vd2mdu	699	15	and	and	CC
cord-014597-66vd2mdu	699	16	development	development	NN
cord-014597-66vd2mdu	699	17	for	for	IN
cord-014597-66vd2mdu	699	18	early	early	JJ
cord-014597-66vd2mdu	699	19	stage	stage	NN
cord-014597-66vd2mdu	699	20	biologics	biologic	NNS
cord-014597-66vd2mdu	699	21	projects	project	NNS
cord-014597-66vd2mdu	699	22	.	.	.
cord-014597-66vd2mdu	700	1	The	the	DT
cord-014597-66vd2mdu	700	2	platform	platform	NN
cord-014597-66vd2mdu	700	3	utilizes	utilize	VBZ
cord-014597-66vd2mdu	700	4	24	24	CD
cord-014597-66vd2mdu	700	5	DWPs	dwp	NNS
cord-014597-66vd2mdu	700	6	,	,	,
cord-014597-66vd2mdu	700	7	NyONE	NyONE	NNP
cord-014597-66vd2mdu	700	8	cell	cell	NN
cord-014597-66vd2mdu	700	9	imager	imager	NN
cord-014597-66vd2mdu	700	10	,	,	.
cord-014597-66vd2mdu	701	1	AMBR15	AMBR15	NNP
cord-014597-66vd2mdu	701	2	,	,	,
cord-014597-66vd2mdu	701	3	Freedom	Freedom	NNP
cord-014597-66vd2mdu	701	4	EVO	EVO	NNP
cord-014597-66vd2mdu	701	5	liquid	liquid	JJ
cord-014597-66vd2mdu	701	6	handler	handler	NN
cord-014597-66vd2mdu	701	7	system	system	NN
cord-014597-66vd2mdu	701	8	,	,	,
cord-014597-66vd2mdu	701	9	and	and	CC
cord-014597-66vd2mdu	701	10	BioHT	BioHT	NNP
cord-014597-66vd2mdu	701	11	metabolic	metabolic	JJ
cord-014597-66vd2mdu	701	12	analyzer	analyzer	NN
cord-014597-66vd2mdu	701	13	to	to	TO
cord-014597-66vd2mdu	701	14	accelerate	accelerate	VB
cord-014597-66vd2mdu	701	15	the	the	DT
cord-014597-66vd2mdu	701	16	screening	screening	NN
cord-014597-66vd2mdu	701	17	process	process	NN
cord-014597-66vd2mdu	701	18	.	.	.
cord-014597-66vd2mdu	702	1	This	this	DT
cord-014597-66vd2mdu	702	2	screening	screening	NN
cord-014597-66vd2mdu	702	3	platform	platform	NN
cord-014597-66vd2mdu	702	4	not	not	RB
cord-014597-66vd2mdu	702	5	only	only	RB
cord-014597-66vd2mdu	702	6	improves	improve	VBZ
cord-014597-66vd2mdu	702	7	process	process	NN
cord-014597-66vd2mdu	702	8	throughput	throughput	NN
cord-014597-66vd2mdu	702	9	,	,	,
cord-014597-66vd2mdu	702	10	operational	operational	JJ
cord-014597-66vd2mdu	702	11	precision	precision	NN
cord-014597-66vd2mdu	702	12	,	,	,
cord-014597-66vd2mdu	702	13	and	and	CC
cord-014597-66vd2mdu	702	14	traceability	traceability	NN
cord-014597-66vd2mdu	702	15	of	of	IN
cord-014597-66vd2mdu	702	16	formulation	formulation	NN
cord-014597-66vd2mdu	702	17	preparation	preparation	NN
cord-014597-66vd2mdu	702	18	,	,	,
cord-014597-66vd2mdu	702	19	but	but	CC
cord-014597-66vd2mdu	702	20	also	also	RB
cord-014597-66vd2mdu	702	21	reduces	reduce	VBZ
cord-014597-66vd2mdu	702	22	the	the	DT
cord-014597-66vd2mdu	702	23	labor	labor	NN
cord-014597-66vd2mdu	702	24	for	for	IN
cord-014597-66vd2mdu	702	25	the	the	DT
cord-014597-66vd2mdu	702	26	media	medium	NNS
cord-014597-66vd2mdu	702	27	and	and	CC
cord-014597-66vd2mdu	702	28	feed	feed	NN
cord-014597-66vd2mdu	702	29	formulation	formulation	NN
cord-014597-66vd2mdu	702	30	preparation	preparation	NN
cord-014597-66vd2mdu	702	31	.	.	.
cord-014597-66vd2mdu	703	1	Background	background	VB
cord-014597-66vd2mdu	703	2	A	a	DT
cord-014597-66vd2mdu	703	3	perfusion	perfusion	NN
cord-014597-66vd2mdu	703	4	medium	medium	NN
cord-014597-66vd2mdu	703	5	requires	require	VBZ
cord-014597-66vd2mdu	703	6	high	high	JJ
cord-014597-66vd2mdu	703	7	concentrations	concentration	NNS
cord-014597-66vd2mdu	703	8	of	of	IN
cord-014597-66vd2mdu	703	9	specific	specific	JJ
cord-014597-66vd2mdu	703	10	nutrients	nutrient	NNS
cord-014597-66vd2mdu	703	11	while	while	IN
cord-014597-66vd2mdu	703	12	balancing	balance	VBG
cord-014597-66vd2mdu	703	13	other	other	JJ
cord-014597-66vd2mdu	703	14	components	component	NNS
cord-014597-66vd2mdu	703	15	to	to	TO
cord-014597-66vd2mdu	703	16	support	support	VB
cord-014597-66vd2mdu	703	17	intensified	intensify	VBN
cord-014597-66vd2mdu	703	18	perfusion	perfusion	NN
cord-014597-66vd2mdu	703	19	processes	process	NNS
cord-014597-66vd2mdu	703	20	.	.	.
cord-014597-66vd2mdu	704	1	Using	use	VBG
cord-014597-66vd2mdu	704	2	a	a	DT
cord-014597-66vd2mdu	704	3	combination	combination	NN
cord-014597-66vd2mdu	704	4	of	of	IN
cord-014597-66vd2mdu	704	5	design	design	NN
cord-014597-66vd2mdu	704	6	of	of	IN
cord-014597-66vd2mdu	704	7	experiment	experiment	NN
cord-014597-66vd2mdu	704	8	(	(	-LRB-
cord-014597-66vd2mdu	704	9	DOE	DOE	NNP
cord-014597-66vd2mdu	704	10	)	)	-RRB-
cord-014597-66vd2mdu	704	11	,	,	,
cord-014597-66vd2mdu	704	12	multivariate	multivariate	JJ
cord-014597-66vd2mdu	704	13	analysis	analysis	NN
cord-014597-66vd2mdu	704	14	(	(	-LRB-
cord-014597-66vd2mdu	704	15	MVA	MVA	NNP
cord-014597-66vd2mdu	704	16	)	)	-RRB-
cord-014597-66vd2mdu	704	17	,	,	,
cord-014597-66vd2mdu	704	18	and	and	CC
cord-014597-66vd2mdu	704	19	spent	spend	VBD
cord-014597-66vd2mdu	704	20	media	medium	NNS
cord-014597-66vd2mdu	704	21	analysis	analysis	NN
cord-014597-66vd2mdu	704	22	,	,	,
cord-014597-66vd2mdu	704	23	we	-PRON-	PRP
cord-014597-66vd2mdu	704	24	developed	develop	VBD
cord-014597-66vd2mdu	704	25	a	a	DT
cord-014597-66vd2mdu	704	26	catalog	catalog	NN
cord-014597-66vd2mdu	704	27	"	"	``
cord-014597-66vd2mdu	704	28	de	de	FW
cord-014597-66vd2mdu	704	29	novo	novo	NN
cord-014597-66vd2mdu	704	30	"	"	''
cord-014597-66vd2mdu	704	31	perfusion	perfusion	NN
cord-014597-66vd2mdu	704	32	medium	medium	NN
cord-014597-66vd2mdu	704	33	by	by	IN
cord-014597-66vd2mdu	704	34	working	work	VBG
cord-014597-66vd2mdu	704	35	with	with	IN
cord-014597-66vd2mdu	704	36	multiple	multiple	JJ
cord-014597-66vd2mdu	704	37	CHO	CHO	NNP
cord-014597-66vd2mdu	704	38	cell	cell	NN
cord-014597-66vd2mdu	704	39	lines	line	NNS
cord-014597-66vd2mdu	704	40	and	and	CC
cord-014597-66vd2mdu	704	41	proteins	protein	NNS
cord-014597-66vd2mdu	704	42	.	.	.
cord-014597-66vd2mdu	705	1	The	the	DT
cord-014597-66vd2mdu	705	2	optimization	optimization	NN
cord-014597-66vd2mdu	705	3	of	of	IN
cord-014597-66vd2mdu	705	4	the	the	DT
cord-014597-66vd2mdu	705	5	medium	medium	NN
cord-014597-66vd2mdu	705	6	in	in	IN
cord-014597-66vd2mdu	705	7	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	705	8	using	use	VBG
cord-014597-66vd2mdu	705	9	alternating	alternate	VBG
cord-014597-66vd2mdu	705	10	tangential	tangential	JJ
cord-014597-66vd2mdu	705	11	flow	flow	NN
cord-014597-66vd2mdu	705	12	(	(	-LRB-
cord-014597-66vd2mdu	705	13	ATF	ATF	NNP
cord-014597-66vd2mdu	705	14	)	)	-RRB-
cord-014597-66vd2mdu	705	15	cell	cell	NN
cord-014597-66vd2mdu	705	16	retention	retention	NN
cord-014597-66vd2mdu	705	17	devices	device	NNS
cord-014597-66vd2mdu	705	18	reduced	reduce	VBD
cord-014597-66vd2mdu	705	19	the	the	DT
cord-014597-66vd2mdu	705	20	minimum	minimum	NN
cord-014597-66vd2mdu	705	21	CSPR	CSPR	NNP
cord-014597-66vd2mdu	705	22	from	from	IN
cord-014597-66vd2mdu	705	23	over	over	IN
cord-014597-66vd2mdu	705	24	80pL	80pl	CD
cord-014597-66vd2mdu	705	25	/	/	SYM
cord-014597-66vd2mdu	705	26	cell	cell	NN
cord-014597-66vd2mdu	705	27	/	/	SYM
cord-014597-66vd2mdu	705	28	d	d	NN
cord-014597-66vd2mdu	705	29	to	to	IN
cord-014597-66vd2mdu	705	30	under	under	IN
cord-014597-66vd2mdu	705	31	35pL	35pl	CD
cord-014597-66vd2mdu	705	32	/	/	SYM
cord-014597-66vd2mdu	705	33	cell	cell	NN
cord-014597-66vd2mdu	705	34	/	/	SYM
cord-014597-66vd2mdu	705	35	d	d	NN
cord-014597-66vd2mdu	705	36	for	for	IN
cord-014597-66vd2mdu	705	37	most	most	JJS
cord-014597-66vd2mdu	705	38	cell	cell	NN
cord-014597-66vd2mdu	705	39	lines	line	NNS
cord-014597-66vd2mdu	705	40	while	while	IN
cord-014597-66vd2mdu	705	41	increasing	increase	VBG
cord-014597-66vd2mdu	705	42	specific	specific	JJ
cord-014597-66vd2mdu	705	43	productivity	productivity	NN
cord-014597-66vd2mdu	705	44	during	during	IN
cord-014597-66vd2mdu	705	45	30	30	CD
cord-014597-66vd2mdu	705	46	day	day	NN
cord-014597-66vd2mdu	705	47	steady	steady	JJ
cord-014597-66vd2mdu	705	48	states	state	NNS
cord-014597-66vd2mdu	705	49	with	with	IN
cord-014597-66vd2mdu	705	50	stable	stable	JJ
cord-014597-66vd2mdu	705	51	growth	growth	NN
cord-014597-66vd2mdu	705	52	rates	rate	NNS
cord-014597-66vd2mdu	705	53	,	,	,
cord-014597-66vd2mdu	705	54	viability	viability	NN
cord-014597-66vd2mdu	705	55	,	,	,
cord-014597-66vd2mdu	705	56	volumetric	volumetric	JJ
cord-014597-66vd2mdu	705	57	productivity	productivity	NN
cord-014597-66vd2mdu	705	58	and	and	CC
cord-014597-66vd2mdu	705	59	product	product	NN
cord-014597-66vd2mdu	705	60	quality	quality	NN
cord-014597-66vd2mdu	705	61	.	.	.
cord-014597-66vd2mdu	706	1	High	high	JJ
cord-014597-66vd2mdu	706	2	Throughput	Throughput	NNP
cord-014597-66vd2mdu	706	3	Screening	Screening	NNP
cord-014597-66vd2mdu	706	4	(	(	-LRB-
cord-014597-66vd2mdu	706	5	HTS	HTS	NNP
cord-014597-66vd2mdu	706	6	)	)	-RRB-
cord-014597-66vd2mdu	706	7	was	be	VBD
cord-014597-66vd2mdu	706	8	performed	perform	VBN
cord-014597-66vd2mdu	706	9	with	with	IN
cord-014597-66vd2mdu	706	10	seven	seven	CD
cord-014597-66vd2mdu	706	11	cell	cell	NN
cord-014597-66vd2mdu	706	12	lines	line	NNS
cord-014597-66vd2mdu	706	13	,	,	,
cord-014597-66vd2mdu	706	14	while	while	IN
cord-014597-66vd2mdu	706	15	four	four	CD
cord-014597-66vd2mdu	706	16	were	be	VBD
cord-014597-66vd2mdu	706	17	used	use	VBN
cord-014597-66vd2mdu	706	18	in	in	IN
cord-014597-66vd2mdu	706	19	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	706	20	:	:	:
cord-014597-66vd2mdu	706	21	CHO	CHO	NNP
cord-014597-66vd2mdu	706	22	-	-	HYPH
cord-014597-66vd2mdu	706	23	S	S	NNP
cord-014597-66vd2mdu	706	24	,	,	,
cord-014597-66vd2mdu	706	25	DG44	DG44	NNP
cord-014597-66vd2mdu	706	26	,	,	,
cord-014597-66vd2mdu	706	27	and	and	CC
cord-014597-66vd2mdu	706	28	two	two	CD
cord-014597-66vd2mdu	706	29	CHOZN	CHOZN	NNP
cord-014597-66vd2mdu	706	30	®	®	NNPS
cord-014597-66vd2mdu	706	31	GS	GS	NNP
cord-014597-66vd2mdu	706	32	lines	line	NNS
cord-014597-66vd2mdu	706	33	,	,	,
cord-014597-66vd2mdu	706	34	each	each	DT
cord-014597-66vd2mdu	706	35	producing	produce	VBG
cord-014597-66vd2mdu	706	36	different	different	JJ
cord-014597-66vd2mdu	706	37	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	706	38	antibodies	antibody	NNS
cord-014597-66vd2mdu	706	39	and	and	CC
cord-014597-66vd2mdu	706	40	include	include	VBP
cord-014597-66vd2mdu	706	41	a	a	DT
cord-014597-66vd2mdu	706	42	fusion	fusion	NN
cord-014597-66vd2mdu	706	43	protein	protein	NN
cord-014597-66vd2mdu	706	44	.	.	.
cord-014597-66vd2mdu	707	1	For	for	IN
cord-014597-66vd2mdu	707	2	HTS	HTS	NNP
cord-014597-66vd2mdu	707	3	experiments	experiment	NNS
cord-014597-66vd2mdu	707	4	,	,	,
cord-014597-66vd2mdu	707	5	cells	cell	NNS
cord-014597-66vd2mdu	707	6	were	be	VBD
cord-014597-66vd2mdu	707	7	inoculated	inoculate	VBN
cord-014597-66vd2mdu	707	8	at	at	IN
cord-014597-66vd2mdu	707	9	2.0x10	2.0x10	CD
cord-014597-66vd2mdu	707	10	6	6	CD
cord-014597-66vd2mdu	707	11	vc	vc	NNP
cord-014597-66vd2mdu	707	12	/	/	SYM
cord-014597-66vd2mdu	707	13	mL	ml	NN
cord-014597-66vd2mdu	707	14	with	with	IN
cord-014597-66vd2mdu	707	15	a	a	DT
cord-014597-66vd2mdu	707	16	30	30	CD
cord-014597-66vd2mdu	707	17	mL	ml	NN
cord-014597-66vd2mdu	707	18	working	working	NN
cord-014597-66vd2mdu	707	19	volume	volume	NN
cord-014597-66vd2mdu	707	20	in	in	IN
cord-014597-66vd2mdu	707	21	50	50	CD
cord-014597-66vd2mdu	707	22	mL	ml	NN
cord-014597-66vd2mdu	707	23	TPP	TPP	NNP
cord-014597-66vd2mdu	707	24	®	®	VBP
cord-014597-66vd2mdu	707	25	tubes	tube	NNS
cord-014597-66vd2mdu	707	26	and	and	CC
cord-014597-66vd2mdu	707	27	cultured	culture	VBN
cord-014597-66vd2mdu	707	28	for	for	IN
cord-014597-66vd2mdu	707	29	7	7	CD
cord-014597-66vd2mdu	707	30	days	day	NNS
cord-014597-66vd2mdu	707	31	in	in	IN
cord-014597-66vd2mdu	707	32	a	a	DT
cord-014597-66vd2mdu	707	33	Multitron	Multitron	NNP
cord-014597-66vd2mdu	707	34	shaken	shake	VBN
cord-014597-66vd2mdu	707	35	at	at	IN
cord-014597-66vd2mdu	707	36	200rpm	200rpm	CD
cord-014597-66vd2mdu	707	37	,	,	,
cord-014597-66vd2mdu	707	38	37	37	CD
cord-014597-66vd2mdu	707	39	°	°	NN
cord-014597-66vd2mdu	707	40	C	c	NN
cord-014597-66vd2mdu	707	41	,	,	,
cord-014597-66vd2mdu	707	42	80	80	CD
cord-014597-66vd2mdu	707	43	%	%	NN
cord-014597-66vd2mdu	707	44	RH	RH	NNP
cord-014597-66vd2mdu	707	45	,	,	,
cord-014597-66vd2mdu	707	46	and	and	CC
cord-014597-66vd2mdu	707	47	5	5	CD
cord-014597-66vd2mdu	707	48	%	%	NN
cord-014597-66vd2mdu	707	49	CO	co	NN
cord-014597-66vd2mdu	707	50	2	2	CD
cord-014597-66vd2mdu	707	51	.	.	.
cord-014597-66vd2mdu	708	1	For	for	IN
cord-014597-66vd2mdu	708	2	benchtop	benchtop	NN
cord-014597-66vd2mdu	708	3	perfusion	perfusion	NN
cord-014597-66vd2mdu	708	4	,	,	,
cord-014597-66vd2mdu	708	5	cells	cell	NNS
cord-014597-66vd2mdu	708	6	were	be	VBD
cord-014597-66vd2mdu	708	7	inoculated	inoculate	VBN
cord-014597-66vd2mdu	708	8	at	at	IN
cord-014597-66vd2mdu	708	9	0.4	0.4	CD
cord-014597-66vd2mdu	708	10	-	-	SYM
cord-014597-66vd2mdu	708	11	2.0x10	2.0x10	CD
cord-014597-66vd2mdu	708	12	6	6	CD
cord-014597-66vd2mdu	708	13	vc	vc	NNP
cord-014597-66vd2mdu	708	14	/	/	SYM
cord-014597-66vd2mdu	708	15	mL	ml	NN
cord-014597-66vd2mdu	708	16	in	in	IN
cord-014597-66vd2mdu	708	17	3L	3l	CD
cord-014597-66vd2mdu	708	18	Applikon	Applikon	NNP
cord-014597-66vd2mdu	708	19	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	708	20	(	(	-LRB-
cord-014597-66vd2mdu	708	21	Applikon	Applikon	NNP
cord-014597-66vd2mdu	708	22	,	,	,
cord-014597-66vd2mdu	708	23	Netherlands	Netherlands	NNP
cord-014597-66vd2mdu	708	24	)	)	-RRB-
cord-014597-66vd2mdu	708	25	with	with	IN
cord-014597-66vd2mdu	708	26	a	a	DT
cord-014597-66vd2mdu	708	27	2L	2l	NN
cord-014597-66vd2mdu	708	28	working	working	NN
cord-014597-66vd2mdu	708	29	volume	volume	NN
cord-014597-66vd2mdu	708	30	.	.	.
cord-014597-66vd2mdu	709	1	Bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	709	2	were	be	VBD
cord-014597-66vd2mdu	709	3	operated	operate	VBN
cord-014597-66vd2mdu	709	4	at	at	IN
cord-014597-66vd2mdu	709	5	350	350	CD
cord-014597-66vd2mdu	709	6	rpm	rpm	NN
cord-014597-66vd2mdu	709	7	,	,	,
cord-014597-66vd2mdu	709	8	37	37	CD
cord-014597-66vd2mdu	709	9	°	°	NN
cord-014597-66vd2mdu	709	10	C	c	NN
cord-014597-66vd2mdu	709	11	,	,	,
cord-014597-66vd2mdu	709	12	40	40	CD
cord-014597-66vd2mdu	709	13	%	%	NN
cord-014597-66vd2mdu	709	14	DO	DO	NNP
cord-014597-66vd2mdu	709	15	,	,	,
cord-014597-66vd2mdu	709	16	and	and	CC
cord-014597-66vd2mdu	709	17	a	a	DT
cord-014597-66vd2mdu	709	18	pH	ph	NN
cord-014597-66vd2mdu	709	19	of	of	IN
cord-014597-66vd2mdu	710	1	6.9	6.9	CD
cord-014597-66vd2mdu	710	2	or	or	CC
cord-014597-66vd2mdu	710	3	7.1±0.05	7.1±0.05	CD
cord-014597-66vd2mdu	710	4	depending	depend	VBG
cord-014597-66vd2mdu	710	5	on	on	IN
cord-014597-66vd2mdu	710	6	the	the	DT
cord-014597-66vd2mdu	710	7	cell	cell	NN
cord-014597-66vd2mdu	710	8	line	line	NN
cord-014597-66vd2mdu	710	9	.	.	.
cord-014597-66vd2mdu	711	1	Oxygen	oxygen	NN
cord-014597-66vd2mdu	711	2	was	be	VBD
cord-014597-66vd2mdu	711	3	supplied	supply	VBN
cord-014597-66vd2mdu	711	4	through	through	IN
cord-014597-66vd2mdu	711	5	an	an	DT
cord-014597-66vd2mdu	711	6	L	l	NN
cord-014597-66vd2mdu	711	7	-	-	HYPH
cord-014597-66vd2mdu	711	8	sparger	sparger	NN
cord-014597-66vd2mdu	711	9	or	or	CC
cord-014597-66vd2mdu	711	10	microsparger	microsparger	NN
cord-014597-66vd2mdu	711	11	as	as	IN
cord-014597-66vd2mdu	711	12	needed	need	VBN
cord-014597-66vd2mdu	711	13	,	,	,
cord-014597-66vd2mdu	711	14	and	and	CC
cord-014597-66vd2mdu	711	15	Excell	Excell	NNP
cord-014597-66vd2mdu	711	16	®	®	POS
cord-014597-66vd2mdu	711	17	antifoam	antifoam	NNP
cord-014597-66vd2mdu	711	18	(	(	-LRB-
cord-014597-66vd2mdu	711	19	MilliporeSigma	MilliporeSigma	NNP
cord-014597-66vd2mdu	711	20	,	,	,
cord-014597-66vd2mdu	711	21	Germany	Germany	NNP
cord-014597-66vd2mdu	711	22	)	)	-RRB-
cord-014597-66vd2mdu	711	23	was	be	VBD
cord-014597-66vd2mdu	711	24	added	add	VBN
cord-014597-66vd2mdu	711	25	at	at	IN
cord-014597-66vd2mdu	711	26	a	a	DT
cord-014597-66vd2mdu	711	27	maximum	maximum	JJ
cord-014597-66vd2mdu	711	28	rate	rate	NN
cord-014597-66vd2mdu	711	29	of	of	IN
cord-014597-66vd2mdu	711	30	0.25	0.25	CD
cord-014597-66vd2mdu	711	31	%	%	NN
cord-014597-66vd2mdu	711	32	v	v	NN
cord-014597-66vd2mdu	711	33	/	/	SYM
cord-014597-66vd2mdu	711	34	v	v	NN
cord-014597-66vd2mdu	711	35	to	to	TO
cord-014597-66vd2mdu	711	36	control	control	VB
cord-014597-66vd2mdu	711	37	foam	foam	NN
cord-014597-66vd2mdu	711	38	.	.	.
cord-014597-66vd2mdu	712	1	At	at	IN
cord-014597-66vd2mdu	712	2	a	a	DT
cord-014597-66vd2mdu	712	3	cell	cell	NN
cord-014597-66vd2mdu	712	4	concentration	concentration	NN
cord-014597-66vd2mdu	712	5	of~6.0x10	of~6.0x10	NNP
cord-014597-66vd2mdu	712	6	6	6	CD
cord-014597-66vd2mdu	712	7	vc	vc	NNP
cord-014597-66vd2mdu	712	8	/	/	SYM
cord-014597-66vd2mdu	712	9	mL	ml	NN
cord-014597-66vd2mdu	712	10	,	,	,
cord-014597-66vd2mdu	712	11	perfusion	perfusion	NN
cord-014597-66vd2mdu	712	12	was	be	VBD
cord-014597-66vd2mdu	712	13	initiated	initiate	VBN
cord-014597-66vd2mdu	712	14	using	use	VBG
cord-014597-66vd2mdu	712	15	the	the	DT
cord-014597-66vd2mdu	712	16	ATF2	ATF2	NNP
cord-014597-66vd2mdu	712	17	(	(	-LRB-
cord-014597-66vd2mdu	712	18	RepliGen	RepliGen	NNP
cord-014597-66vd2mdu	712	19	,	,	,
cord-014597-66vd2mdu	712	20	Massachusetts	Massachusetts	NNP
cord-014597-66vd2mdu	712	21	)	)	-RRB-
cord-014597-66vd2mdu	712	22	,	,	,
cord-014597-66vd2mdu	712	23	with	with	IN
cord-014597-66vd2mdu	712	24	a	a	DT
cord-014597-66vd2mdu	712	25	bleed	bleed	NN
cord-014597-66vd2mdu	712	26	set	set	VBN
cord-014597-66vd2mdu	712	27	to	to	TO
cord-014597-66vd2mdu	712	28	maintain	maintain	VB
cord-014597-66vd2mdu	712	29	cell	cell	NN
cord-014597-66vd2mdu	712	30	concentrations	concentration	NNS
cord-014597-66vd2mdu	712	31	at	at	IN
cord-014597-66vd2mdu	712	32	50	50	CD
cord-014597-66vd2mdu	712	33	or	or	CC
cord-014597-66vd2mdu	712	34	80	80	CD
cord-014597-66vd2mdu	712	35	*	*	SYM
cord-014597-66vd2mdu	712	36	10	10	CD
cord-014597-66vd2mdu	712	37	6	6	CD
cord-014597-66vd2mdu	712	38	vc	vc	NNP
cord-014597-66vd2mdu	712	39	/	/	SYM
cord-014597-66vd2mdu	712	40	mL.	mL.	NNP
cord-014597-66vd2mdu	712	41	Two	two	CD
cord-014597-66vd2mdu	712	42	"	"	``
cord-014597-66vd2mdu	712	43	de	de	FW
cord-014597-66vd2mdu	712	44	novo	novo	NNP
cord-014597-66vd2mdu	712	45	"	"	''
cord-014597-66vd2mdu	712	46	prototype	prototype	NN
cord-014597-66vd2mdu	712	47	media	medium	NNS
cord-014597-66vd2mdu	712	48	were	be	VBD
cord-014597-66vd2mdu	712	49	developed	develop	VBN
cord-014597-66vd2mdu	712	50	using	use	VBG
cord-014597-66vd2mdu	712	51	DOE	DOE	NNP
cord-014597-66vd2mdu	712	52	and	and	CC
cord-014597-66vd2mdu	712	53	MVA	MVA	NNP
cord-014597-66vd2mdu	712	54	in	in	IN
cord-014597-66vd2mdu	712	55	HTS	HTS	NNP
cord-014597-66vd2mdu	712	56	with	with	IN
cord-014597-66vd2mdu	712	57	TPPs	tpp	NNS
cord-014597-66vd2mdu	712	58	and	and	CC
cord-014597-66vd2mdu	712	59	an	an	DT
cord-014597-66vd2mdu	712	60	ambr	ambr	NN
cord-014597-66vd2mdu	712	61	®	®	NN
cord-014597-66vd2mdu	712	62	15	15	CD
cord-014597-66vd2mdu	712	63	[	[	-LRB-
cord-014597-66vd2mdu	712	64	1	1	CD
cord-014597-66vd2mdu	712	65	]	]	-RRB-
cord-014597-66vd2mdu	712	66	and	and	CC
cord-014597-66vd2mdu	712	67	one	one	PRP
cord-014597-66vd2mdu	712	68	was	be	VBD
cord-014597-66vd2mdu	712	69	chosen	choose	VBN
cord-014597-66vd2mdu	712	70	for	for	IN
cord-014597-66vd2mdu	712	71	further	further	JJ
cord-014597-66vd2mdu	712	72	development	development	NN
cord-014597-66vd2mdu	712	73	after	after	IN
cord-014597-66vd2mdu	712	74	comparing	compare	VBG
cord-014597-66vd2mdu	712	75	to	to	IN
cord-014597-66vd2mdu	712	76	a	a	DT
cord-014597-66vd2mdu	712	77	basal	basal	NN
cord-014597-66vd2mdu	712	78	medium	medium	NN
cord-014597-66vd2mdu	712	79	enriched	enrich	VBN
cord-014597-66vd2mdu	712	80	with	with	IN
cord-014597-66vd2mdu	712	81	feed	feed	NN
cord-014597-66vd2mdu	712	82	in	in	IN
cord-014597-66vd2mdu	712	83	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	712	84	.	.	.
cord-014597-66vd2mdu	713	1	Eleven	eleven	CD
cord-014597-66vd2mdu	713	2	components	component	NNS
cord-014597-66vd2mdu	713	3	were	be	VBD
cord-014597-66vd2mdu	713	4	identified	identify	VBN
cord-014597-66vd2mdu	713	5	as	as	IN
cord-014597-66vd2mdu	713	6	significant	significant	JJ
cord-014597-66vd2mdu	713	7	effectors	effector	NNS
cord-014597-66vd2mdu	713	8	of	of	IN
cord-014597-66vd2mdu	713	9	critical	critical	JJ
cord-014597-66vd2mdu	713	10	parameters	parameter	NNS
cord-014597-66vd2mdu	713	11	for	for	IN
cord-014597-66vd2mdu	713	12	perfusion	perfusion	NN
cord-014597-66vd2mdu	713	13	processes	process	NNS
cord-014597-66vd2mdu	713	14	across	across	IN
cord-014597-66vd2mdu	713	15	evaluated	evaluate	VBN
cord-014597-66vd2mdu	713	16	cell	cell	NN
cord-014597-66vd2mdu	713	17	lines	line	NNS
cord-014597-66vd2mdu	713	18	.	.	.
cord-014597-66vd2mdu	714	1	DOE	DOE	NNP
cord-014597-66vd2mdu	714	2	central	central	JJ
cord-014597-66vd2mdu	714	3	composite	composite	JJ
cord-014597-66vd2mdu	714	4	experiments	experiment	NNS
cord-014597-66vd2mdu	714	5	were	be	VBD
cord-014597-66vd2mdu	714	6	run	run	VBN
cord-014597-66vd2mdu	714	7	and	and	CC
cord-014597-66vd2mdu	714	8	component	component	NN
cord-014597-66vd2mdu	714	9	concentrations	concentration	NNS
cord-014597-66vd2mdu	714	10	were	be	VBD
cord-014597-66vd2mdu	714	11	optimized	optimize	VBN
cord-014597-66vd2mdu	714	12	in	in	IN
cord-014597-66vd2mdu	714	13	the	the	DT
cord-014597-66vd2mdu	714	14	selected	select	VBN
cord-014597-66vd2mdu	714	15	prototype	prototype	NN
cord-014597-66vd2mdu	714	16	.	.	.
cord-014597-66vd2mdu	715	1	In	in	IN
cord-014597-66vd2mdu	715	2	parallel	parallel	JJ
cord-014597-66vd2mdu	715	3	,	,	,
cord-014597-66vd2mdu	715	4	amino	amino	NN
cord-014597-66vd2mdu	715	5	acid	acid	NN
cord-014597-66vd2mdu	715	6	specific	specific	JJ
cord-014597-66vd2mdu	715	7	consumption	consumption	NN
cord-014597-66vd2mdu	715	8	rates	rate	NNS
cord-014597-66vd2mdu	715	9	were	be	VBD
cord-014597-66vd2mdu	715	10	calculated	calculate	VBN
cord-014597-66vd2mdu	715	11	from	from	IN
cord-014597-66vd2mdu	715	12	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	715	13	spent	spend	VBN
cord-014597-66vd2mdu	715	14	media	medium	NNS
cord-014597-66vd2mdu	715	15	samples	sample	NNS
cord-014597-66vd2mdu	715	16	and	and	CC
cord-014597-66vd2mdu	715	17	used	use	VBD
cord-014597-66vd2mdu	715	18	to	to	TO
cord-014597-66vd2mdu	715	19	adjust	adjust	VB
cord-014597-66vd2mdu	715	20	the	the	DT
cord-014597-66vd2mdu	715	21	concentration	concentration	NN
cord-014597-66vd2mdu	715	22	of	of	IN
cord-014597-66vd2mdu	715	23	amino	amino	JJ
cord-014597-66vd2mdu	715	24	acids	acid	NNS
cord-014597-66vd2mdu	715	25	to	to	TO
cord-014597-66vd2mdu	715	26	target	target	VB
cord-014597-66vd2mdu	715	27	a	a	DT
cord-014597-66vd2mdu	715	28	reduced	reduce	VBN
cord-014597-66vd2mdu	715	29	CSPR	CSPR	NNP
cord-014597-66vd2mdu	715	30	.	.	.
cord-014597-66vd2mdu	716	1	Increasing	increase	VBG
cord-014597-66vd2mdu	716	2	specific	specific	JJ
cord-014597-66vd2mdu	716	3	amino	amino	NN
cord-014597-66vd2mdu	716	4	acids	acid	NNS
cord-014597-66vd2mdu	716	5	concentrations	concentration	NNS
cord-014597-66vd2mdu	716	6	resulted	result	VBD
cord-014597-66vd2mdu	716	7	in	in	IN
cord-014597-66vd2mdu	716	8	a	a	DT
cord-014597-66vd2mdu	716	9	significant	significant	JJ
cord-014597-66vd2mdu	716	10	reduction	reduction	NN
cord-014597-66vd2mdu	716	11	of	of	IN
cord-014597-66vd2mdu	716	12	the	the	DT
cord-014597-66vd2mdu	716	13	minimum	minimum	NN
cord-014597-66vd2mdu	716	14	CSPR	CSPR	NNP
cord-014597-66vd2mdu	716	15	across	across	IN
cord-014597-66vd2mdu	716	16	all	all	DT
cord-014597-66vd2mdu	716	17	tested	test	VBN
cord-014597-66vd2mdu	716	18	cell	cell	NN
cord-014597-66vd2mdu	716	19	lines	line	NNS
cord-014597-66vd2mdu	716	20	-for	-for	.
cord-014597-66vd2mdu	717	1	example	example	NN
cord-014597-66vd2mdu	718	1	the	the	DT
cord-014597-66vd2mdu	718	2	CSPR	CSPR	NNP
cord-014597-66vd2mdu	718	3	of	of	IN
cord-014597-66vd2mdu	718	4	CHO	CHO	NNP
cord-014597-66vd2mdu	718	5	-	-	HYPH
cord-014597-66vd2mdu	718	6	S	S	NNP
cord-014597-66vd2mdu	718	7	was	be	VBD
cord-014597-66vd2mdu	718	8	reduced	reduce	VBN
cord-014597-66vd2mdu	718	9	from	from	IN
cord-014597-66vd2mdu	718	10	60	60	CD
cord-014597-66vd2mdu	718	11	to	to	IN
cord-014597-66vd2mdu	718	12	39pL	39pl	CD
cord-014597-66vd2mdu	718	13	/	/	SYM
cord-014597-66vd2mdu	718	14	cell	cell	NN
cord-014597-66vd2mdu	718	15	/	/	SYM
cord-014597-66vd2mdu	718	16	day	day	NN
cord-014597-66vd2mdu	718	17	(	(	-LRB-
cord-014597-66vd2mdu	718	18	Table	Table	NNP
cord-014597-66vd2mdu	718	19	1	1	CD
cord-014597-66vd2mdu	718	20	)	)	-RRB-
cord-014597-66vd2mdu	718	21	.	.	.
cord-014597-66vd2mdu	719	1	However	however	RB
cord-014597-66vd2mdu	719	2	,	,	,
cord-014597-66vd2mdu	719	3	even	even	RB
cord-014597-66vd2mdu	719	4	at	at	IN
cord-014597-66vd2mdu	719	5	the	the	DT
cord-014597-66vd2mdu	719	6	lower	low	JJR
cord-014597-66vd2mdu	719	7	CSPR	CSPR	NNP
cord-014597-66vd2mdu	719	8	,	,	,
cord-014597-66vd2mdu	719	9	spent	spend	VBD
cord-014597-66vd2mdu	719	10	media	medium	NNS
cord-014597-66vd2mdu	719	11	analysis	analysis	NN
cord-014597-66vd2mdu	719	12	revealed	reveal	VBD
cord-014597-66vd2mdu	719	13	excess	excess	JJ
cord-014597-66vd2mdu	719	14	concentration	concentration	NN
cord-014597-66vd2mdu	719	15	of	of	IN
cord-014597-66vd2mdu	719	16	some	some	DT
cord-014597-66vd2mdu	719	17	amino	amino	NN
cord-014597-66vd2mdu	719	18	acids	acid	NNS
cord-014597-66vd2mdu	719	19	,	,	,
cord-014597-66vd2mdu	719	20	so	so	RB
cord-014597-66vd2mdu	719	21	specific	specific	JJ
cord-014597-66vd2mdu	719	22	accumulating	accumulate	VBG
cord-014597-66vd2mdu	719	23	amino	amino	NN
cord-014597-66vd2mdu	719	24	acids	acid	NNS
cord-014597-66vd2mdu	719	25	were	be	VBD
cord-014597-66vd2mdu	719	26	reduced	reduce	VBN
cord-014597-66vd2mdu	719	27	and	and	CC
cord-014597-66vd2mdu	719	28	components	component	NNS
cord-014597-66vd2mdu	719	29	were	be	VBD
cord-014597-66vd2mdu	719	30	streamlined	streamline	VBN
cord-014597-66vd2mdu	719	31	for	for	IN
cord-014597-66vd2mdu	719	32	the	the	DT
cord-014597-66vd2mdu	719	33	final	final	JJ
cord-014597-66vd2mdu	719	34	medium	medium	NN
cord-014597-66vd2mdu	719	35	:	:	:
cord-014597-66vd2mdu	719	36	EXCELL	EXCELL	NNP
cord-014597-66vd2mdu	719	37	®	®	NNP
cord-014597-66vd2mdu	719	38	Advanced	advanced	JJ
cord-014597-66vd2mdu	719	39	HD	HD	NNP
cord-014597-66vd2mdu	719	40	Perfusion	Perfusion	NNP
cord-014597-66vd2mdu	719	41	Medium	Medium	NNP
cord-014597-66vd2mdu	719	42	.	.	.
cord-014597-66vd2mdu	720	1	Using	use	VBG
cord-014597-66vd2mdu	720	2	this	this	DT
cord-014597-66vd2mdu	720	3	medium	medium	NN
cord-014597-66vd2mdu	720	4	,	,	,
cord-014597-66vd2mdu	720	5	a	a	DT
cord-014597-66vd2mdu	720	6	CHO	CHO	NNP
cord-014597-66vd2mdu	720	7	-	-	HYPH
cord-014597-66vd2mdu	720	8	S	S	NNP
cord-014597-66vd2mdu	720	9	and	and	CC
cord-014597-66vd2mdu	720	10	a	a	DT
cord-014597-66vd2mdu	720	11	CHOZN	CHOZN	NNP
cord-014597-66vd2mdu	720	12	®	®	NN
cord-014597-66vd2mdu	720	13	GS	GS	NNP
cord-014597-66vd2mdu	720	14	cell	cell	NN
cord-014597-66vd2mdu	720	15	line	line	NN
cord-014597-66vd2mdu	720	16	producing	produce	VBG
cord-014597-66vd2mdu	720	17	a	a	DT
cord-014597-66vd2mdu	720	18	fusion	fusion	NN
cord-014597-66vd2mdu	720	19	protein	protein	NN
cord-014597-66vd2mdu	720	20	were	be	VBD
cord-014597-66vd2mdu	720	21	cultured	culture	VBN
cord-014597-66vd2mdu	720	22	at	at	IN
cord-014597-66vd2mdu	720	23	a	a	DT
cord-014597-66vd2mdu	720	24	CSPR	CSPR	NNP
cord-014597-66vd2mdu	720	25	of	of	IN
cord-014597-66vd2mdu	720	26	less	less	JJR
cord-014597-66vd2mdu	720	27	than	than	IN
cord-014597-66vd2mdu	720	28	40pL	40pl	CD
cord-014597-66vd2mdu	720	29	/	/	SYM
cord-014597-66vd2mdu	720	30	cell	cell	NN
cord-014597-66vd2mdu	720	31	/	/	SYM
cord-014597-66vd2mdu	720	32	day	day	NN
cord-014597-66vd2mdu	720	33	with	with	IN
cord-014597-66vd2mdu	720	34	a	a	DT
cord-014597-66vd2mdu	720	35	VCD	vcd	NN
cord-014597-66vd2mdu	720	36	of	of	IN
cord-014597-66vd2mdu	720	37	50	50	CD
cord-014597-66vd2mdu	720	38	*	*	NFP
cord-014597-66vd2mdu	720	39	10	10	CD
cord-014597-66vd2mdu	720	40	6	6	CD
cord-014597-66vd2mdu	720	41	vc	vc	NNP
cord-014597-66vd2mdu	720	42	/	/	SYM
cord-014597-66vd2mdu	720	43	mL.	mL.	NNP
cord-014597-66vd2mdu	720	44	Metabolic	metabolic	JJ
cord-014597-66vd2mdu	720	45	profile	profile	NN
cord-014597-66vd2mdu	720	46	,	,	,
cord-014597-66vd2mdu	720	47	productivity	productivity	NN
cord-014597-66vd2mdu	720	48	,	,	,
cord-014597-66vd2mdu	720	49	and	and	CC
cord-014597-66vd2mdu	720	50	product	product	NN
cord-014597-66vd2mdu	720	51	quality	quality	NN
cord-014597-66vd2mdu	720	52	were	be	VBD
cord-014597-66vd2mdu	720	53	constant	constant	JJ
cord-014597-66vd2mdu	720	54	over	over	IN
cord-014597-66vd2mdu	720	55	the	the	DT
cord-014597-66vd2mdu	720	56	30	30	CD
cord-014597-66vd2mdu	720	57	day	day	NN
cord-014597-66vd2mdu	720	58	steady	steady	JJ
cord-014597-66vd2mdu	720	59	state	state	NN
cord-014597-66vd2mdu	720	60	.	.	.
cord-014597-66vd2mdu	721	1	The	the	DT
cord-014597-66vd2mdu	721	2	CHOZN	CHOZN	NNP
cord-014597-66vd2mdu	721	3	®	®	NN
cord-014597-66vd2mdu	721	4	GS	GS	NNP
cord-014597-66vd2mdu	721	5	cell	cell	NN
cord-014597-66vd2mdu	721	6	line	line	NN
cord-014597-66vd2mdu	721	7	was	be	VBD
cord-014597-66vd2mdu	721	8	also	also	RB
cord-014597-66vd2mdu	721	9	tested	test	VBN
cord-014597-66vd2mdu	721	10	at	at	IN
cord-014597-66vd2mdu	721	11	80	80	CD
cord-014597-66vd2mdu	721	12	*	*	SYM
cord-014597-66vd2mdu	721	13	10	10	CD
cord-014597-66vd2mdu	721	14	6	6	CD
cord-014597-66vd2mdu	721	15	vc	vc	NN
cord-014597-66vd2mdu	721	16	/	/	SYM
cord-014597-66vd2mdu	721	17	mL	ml	NN
cord-014597-66vd2mdu	721	18	with	with	IN
cord-014597-66vd2mdu	721	19	a	a	DT
cord-014597-66vd2mdu	721	20	CSPR	CSPR	NNP
cord-014597-66vd2mdu	721	21	of	of	IN
cord-014597-66vd2mdu	721	22	33pL	33pl	CD
cord-014597-66vd2mdu	721	23	/	/	SYM
cord-014597-66vd2mdu	721	24	cell	cell	NN
cord-014597-66vd2mdu	721	25	/	/	SYM
cord-014597-66vd2mdu	721	26	day	day	NN
cord-014597-66vd2mdu	722	1	(	(	-LRB-
cord-014597-66vd2mdu	722	2	Fig	Fig	NNP
cord-014597-66vd2mdu	722	3	.	.	NNP
cord-014597-66vd2mdu	722	4	1	1	CD
cord-014597-66vd2mdu	722	5	)	)	-RRB-
cord-014597-66vd2mdu	722	6	.	.	.
cord-014597-66vd2mdu	723	1	We	-PRON-	PRP
cord-014597-66vd2mdu	723	2	have	have	VBP
cord-014597-66vd2mdu	723	3	developed	develop	VBN
cord-014597-66vd2mdu	723	4	a	a	DT
cord-014597-66vd2mdu	723	5	catalog	catalog	NN
cord-014597-66vd2mdu	723	6	perfusion	perfusion	NN
cord-014597-66vd2mdu	723	7	medium	medium	NN
cord-014597-66vd2mdu	723	8	from	from	IN
cord-014597-66vd2mdu	723	9	first	first	JJ
cord-014597-66vd2mdu	723	10	principles	principle	NNS
cord-014597-66vd2mdu	723	11	,	,	,
cord-014597-66vd2mdu	723	12	ensuring	ensure	VBG
cord-014597-66vd2mdu	723	13	broadness	broadness	NN
cord-014597-66vd2mdu	723	14	of	of	IN
cord-014597-66vd2mdu	723	15	application	application	NN
cord-014597-66vd2mdu	723	16	by	by	IN
cord-014597-66vd2mdu	723	17	using	use	VBG
cord-014597-66vd2mdu	723	18	seven	seven	CD
cord-014597-66vd2mdu	723	19	cell	cell	NN
cord-014597-66vd2mdu	723	20	lines	line	NNS
cord-014597-66vd2mdu	723	21	in	in	IN
cord-014597-66vd2mdu	723	22	scaleddown	scaleddown	NN
cord-014597-66vd2mdu	723	23	systems	system	NNS
cord-014597-66vd2mdu	723	24	and	and	CC
cord-014597-66vd2mdu	723	25	four	four	CD
cord-014597-66vd2mdu	723	26	in	in	IN
cord-014597-66vd2mdu	723	27	perfusion	perfusion	NN
cord-014597-66vd2mdu	723	28	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	723	29	.	.	.
cord-014597-66vd2mdu	724	1	The	the	DT
cord-014597-66vd2mdu	724	2	final	final	JJ
cord-014597-66vd2mdu	724	3	catalog	catalog	NN
cord-014597-66vd2mdu	724	4	medium	medium	NN
cord-014597-66vd2mdu	724	5	showed	show	VBD
cord-014597-66vd2mdu	724	6	significant	significant	JJ
cord-014597-66vd2mdu	724	7	improvements	improvement	NNS
cord-014597-66vd2mdu	724	8	in	in	IN
cord-014597-66vd2mdu	724	9	productivity	productivity	NN
cord-014597-66vd2mdu	724	10	across	across	IN
cord-014597-66vd2mdu	724	11	all	all	DT
cord-014597-66vd2mdu	724	12	cell	cell	NN
cord-014597-66vd2mdu	724	13	lines	line	NNS
cord-014597-66vd2mdu	724	14	,	,	,
cord-014597-66vd2mdu	724	15	with	with	IN
cord-014597-66vd2mdu	724	16	reduced	reduce	VBN
cord-014597-66vd2mdu	724	17	CSPRs	cspr	NNS
cord-014597-66vd2mdu	724	18	when	when	WRB
cord-014597-66vd2mdu	724	19	compared	compare	VBN
cord-014597-66vd2mdu	724	20	to	to	IN
cord-014597-66vd2mdu	724	21	enriched	enrich	VBN
cord-014597-66vd2mdu	724	22	fed	fed	NNP
cord-014597-66vd2mdu	724	23	-	-	HYPH
cord-014597-66vd2mdu	724	24	batch	batch	NN
cord-014597-66vd2mdu	724	25	medium	medium	NN
cord-014597-66vd2mdu	724	26	or	or	CC
cord-014597-66vd2mdu	724	27	initial	initial	JJ
cord-014597-66vd2mdu	724	28	prototypes	prototype	NNS
cord-014597-66vd2mdu	724	29	(	(	-LRB-
cord-014597-66vd2mdu	724	30	Table	table	NN
cord-014597-66vd2mdu	724	31	1	1	CD
cord-014597-66vd2mdu	724	32	)	)	-RRB-
cord-014597-66vd2mdu	724	33	.	.	.
cord-014597-66vd2mdu	725	1	There	there	EX
cord-014597-66vd2mdu	725	2	is	be	VBZ
cord-014597-66vd2mdu	725	3	a	a	DT
cord-014597-66vd2mdu	725	4	rising	rise	VBG
cord-014597-66vd2mdu	725	5	demand	demand	NN
cord-014597-66vd2mdu	725	6	for	for	IN
cord-014597-66vd2mdu	725	7	accelerated	accelerated	JJ
cord-014597-66vd2mdu	725	8	process	process	NN
cord-014597-66vd2mdu	725	9	development	development	NN
cord-014597-66vd2mdu	725	10	,	,	,
cord-014597-66vd2mdu	725	11	increased	increase	VBN
cord-014597-66vd2mdu	725	12	efficiency	efficiency	NN
cord-014597-66vd2mdu	725	13	and	and	CC
cord-014597-66vd2mdu	725	14	economics	economic	NNS
cord-014597-66vd2mdu	725	15	for	for	IN
cord-014597-66vd2mdu	725	16	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	725	17	production	production	NN
cord-014597-66vd2mdu	725	18	processes	process	NNS
cord-014597-66vd2mdu	725	19	.	.	.
cord-014597-66vd2mdu	726	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	726	2	,	,	,
cord-014597-66vd2mdu	726	3	increased	increase	VBN
cord-014597-66vd2mdu	726	4	process	process	NN
cord-014597-66vd2mdu	726	5	understanding	understanding	NN
cord-014597-66vd2mdu	726	6	have	have	VBP
cord-014597-66vd2mdu	726	7	evolved	evolve	VBN
cord-014597-66vd2mdu	726	8	from	from	IN
cord-014597-66vd2mdu	726	9	the	the	DT
cord-014597-66vd2mdu	726	10	Process	Process	NNP
cord-014597-66vd2mdu	726	11	Analytical	Analytical	NNP
cord-014597-66vd2mdu	726	12	Tool	Tool	NNP
cord-014597-66vd2mdu	726	13	initiative	initiative	NN
cord-014597-66vd2mdu	726	14	(	(	-LRB-
cord-014597-66vd2mdu	726	15	PAT	PAT	NNP
cord-014597-66vd2mdu	726	16	)	)	-RRB-
cord-014597-66vd2mdu	726	17	and	and	CC
cord-014597-66vd2mdu	726	18	the	the	DT
cord-014597-66vd2mdu	726	19	Quality	Quality	NNP
cord-014597-66vd2mdu	726	20	by	by	IN
cord-014597-66vd2mdu	726	21	Design	Design	NNP
cord-014597-66vd2mdu	726	22	(	(	-LRB-
cord-014597-66vd2mdu	726	23	QbD	qbd	NN
cord-014597-66vd2mdu	726	24	)	)	-RRB-
cord-014597-66vd2mdu	726	25	methodology	methodology	NN
cord-014597-66vd2mdu	726	26	.	.	.
cord-014597-66vd2mdu	727	1	In	in	IN
cord-014597-66vd2mdu	727	2	contrast	contrast	NN
cord-014597-66vd2mdu	727	3	to	to	IN
cord-014597-66vd2mdu	727	4	one	one	CD
cord-014597-66vd2mdu	727	5	-	-	HYPH
cord-014597-66vd2mdu	727	6	factor	factor	NN
cord-014597-66vd2mdu	727	7	-	-	HYPH
cord-014597-66vd2mdu	727	8	at	at	IN
cord-014597-66vd2mdu	727	9	-	-	HYPH
cord-014597-66vd2mdu	727	10	atime	atime	NN
cord-014597-66vd2mdu	727	11	methods	method	NNS
cord-014597-66vd2mdu	727	12	,	,	,
cord-014597-66vd2mdu	727	13	statistical	statistical	JJ
cord-014597-66vd2mdu	727	14	Design	Design	NNP
cord-014597-66vd2mdu	727	15	of	of	IN
cord-014597-66vd2mdu	727	16	Experiment	Experiment	NNP
cord-014597-66vd2mdu	727	17	(	(	-LRB-
cord-014597-66vd2mdu	727	18	DoE	DoE	NNP
cord-014597-66vd2mdu	727	19	)	)	-RRB-
cord-014597-66vd2mdu	727	20	methods	method	NNS
cord-014597-66vd2mdu	727	21	are	be	VBP
cord-014597-66vd2mdu	727	22	widely	widely	RB
cord-014597-66vd2mdu	727	23	used	use	VBN
cord-014597-66vd2mdu	727	24	to	to	TO
cord-014597-66vd2mdu	727	25	develop	develop	VB
cord-014597-66vd2mdu	727	26	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	727	27	processes	process	NNS
cord-014597-66vd2mdu	727	28	.	.	.
cord-014597-66vd2mdu	728	1	Even	even	RB
cord-014597-66vd2mdu	728	2	if	if	IN
cord-014597-66vd2mdu	728	3	highthroughput	highthroughput	NN
cord-014597-66vd2mdu	728	4	systems	system	NNS
cord-014597-66vd2mdu	728	5	can	can	MD
cord-014597-66vd2mdu	728	6	handle	handle	VB
cord-014597-66vd2mdu	728	7	these	these	DT
cord-014597-66vd2mdu	728	8	numbers	number	NNS
cord-014597-66vd2mdu	728	9	of	of	IN
cord-014597-66vd2mdu	728	10	experiments	experiment	NNS
cord-014597-66vd2mdu	728	11	in	in	IN
cord-014597-66vd2mdu	728	12	parallel	parallel	NN
cord-014597-66vd2mdu	728	13	,	,	,
cord-014597-66vd2mdu	728	14	the	the	DT
cord-014597-66vd2mdu	728	15	heuristic	heuristic	JJ
cord-014597-66vd2mdu	728	16	restriction	restriction	NN
cord-014597-66vd2mdu	728	17	of	of	IN
cord-014597-66vd2mdu	728	18	boundaries	boundary	NNS
cord-014597-66vd2mdu	728	19	and	and	CC
cord-014597-66vd2mdu	728	20	the	the	DT
cord-014597-66vd2mdu	728	21	high	high	JJ
cord-014597-66vd2mdu	728	22	number	number	NN
cord-014597-66vd2mdu	728	23	of	of	IN
cord-014597-66vd2mdu	728	24	factors	factor	NNS
cord-014597-66vd2mdu	728	25	results	result	NNS
cord-014597-66vd2mdu	728	26	in	in	IN
cord-014597-66vd2mdu	728	27	stepwise	stepwise	JJ
cord-014597-66vd2mdu	728	28	iterations	iteration	NNS
cord-014597-66vd2mdu	728	29	with	with	IN
cord-014597-66vd2mdu	728	30	multiple	multiple	JJ
cord-014597-66vd2mdu	728	31	runs	run	NNS
cord-014597-66vd2mdu	728	32	.	.	.
cord-014597-66vd2mdu	729	1	Therefore	therefore	RB
cord-014597-66vd2mdu	729	2	,	,	,
cord-014597-66vd2mdu	729	3	the	the	DT
cord-014597-66vd2mdu	729	4	combination	combination	NN
cord-014597-66vd2mdu	729	5	of	of	IN
cord-014597-66vd2mdu	729	6	model	model	NN
cord-014597-66vd2mdu	729	7	-	-	HYPH
cord-014597-66vd2mdu	729	8	based	base	VBN
cord-014597-66vd2mdu	729	9	simulations	simulation	NNS
cord-014597-66vd2mdu	729	10	with	with	IN
cord-014597-66vd2mdu	729	11	DoE	DoE	NNP
cord-014597-66vd2mdu	729	12	methods	method	NNS
cord-014597-66vd2mdu	729	13	(	(	-LRB-
cord-014597-66vd2mdu	729	14	mDoE	mdoe	CD
cord-014597-66vd2mdu	729	15	)	)	-RRB-
cord-014597-66vd2mdu	729	16	for	for	IN
cord-014597-66vd2mdu	729	17	the	the	DT
cord-014597-66vd2mdu	729	18	development	development	NN
cord-014597-66vd2mdu	729	19	of	of	IN
cord-014597-66vd2mdu	729	20	sophisticated	sophisticated	JJ
cord-014597-66vd2mdu	729	21	cell	cell	NN
cord-014597-66vd2mdu	729	22	culture	culture	NN
cord-014597-66vd2mdu	729	23	processes	process	NNS
cord-014597-66vd2mdu	729	24	is	be	VBZ
cord-014597-66vd2mdu	729	25	a	a	DT
cord-014597-66vd2mdu	729	26	novel	novel	JJ
cord-014597-66vd2mdu	729	27	tool	tool	NN
cord-014597-66vd2mdu	729	28	for	for	IN
cord-014597-66vd2mdu	729	29	process	process	NN
cord-014597-66vd2mdu	729	30	development	development	NN
cord-014597-66vd2mdu	729	31	[	[	-LRB-
cord-014597-66vd2mdu	729	32	1	1	CD
cord-014597-66vd2mdu	729	33	]	]	-RRB-
cord-014597-66vd2mdu	729	34	.	.	.
cord-014597-66vd2mdu	730	1	It	-PRON-	PRP
cord-014597-66vd2mdu	730	2	is	be	VBZ
cord-014597-66vd2mdu	730	3	used	use	VBN
cord-014597-66vd2mdu	730	4	to	to	TO
cord-014597-66vd2mdu	730	5	reduce	reduce	VB
cord-014597-66vd2mdu	730	6	the	the	DT
cord-014597-66vd2mdu	730	7	number	number	NN
cord-014597-66vd2mdu	730	8	of	of	IN
cord-014597-66vd2mdu	730	9	experiments	experiment	NNS
cord-014597-66vd2mdu	730	10	during	during	IN
cord-014597-66vd2mdu	730	11	DoE	DoE	NNP
cord-014597-66vd2mdu	730	12	and	and	CC
cord-014597-66vd2mdu	730	13	the	the	DT
cord-014597-66vd2mdu	730	14	time	time	NN
cord-014597-66vd2mdu	730	15	needed	need	VBN
cord-014597-66vd2mdu	730	16	for	for	IN
cord-014597-66vd2mdu	730	17	the	the	DT
cord-014597-66vd2mdu	730	18	development	development	NN
cord-014597-66vd2mdu	730	19	of	of	IN
cord-014597-66vd2mdu	730	20	more	more	JJR
cord-014597-66vd2mdu	730	21	knowledge	knowledge	NN
cord-014597-66vd2mdu	730	22	-	-	HYPH
cord-014597-66vd2mdu	730	23	based	base	VBN
cord-014597-66vd2mdu	730	24	cell	cell	NN
cord-014597-66vd2mdu	730	25	culture	culture	NN
cord-014597-66vd2mdu	730	26	processes	process	NNS
cord-014597-66vd2mdu	730	27	.	.	.
cord-014597-66vd2mdu	731	1	This	this	DT
cord-014597-66vd2mdu	731	2	concept	concept	NN
cord-014597-66vd2mdu	731	3	was	be	VBD
cord-014597-66vd2mdu	731	4	applied	apply	VBN
cord-014597-66vd2mdu	731	5	to	to	IN
cord-014597-66vd2mdu	731	6	the	the	DT
cord-014597-66vd2mdu	731	7	optimization	optimization	NN
cord-014597-66vd2mdu	731	8	of	of	IN
cord-014597-66vd2mdu	731	9	the	the	DT
cord-014597-66vd2mdu	731	10	initial	initial	JJ
cord-014597-66vd2mdu	731	11	glutamine	glutamine	NN
cord-014597-66vd2mdu	731	12	and	and	CC
cord-014597-66vd2mdu	731	13	glucose	glucose	NN
cord-014597-66vd2mdu	731	14	concentrations	concentration	NNS
cord-014597-66vd2mdu	731	15	of	of	IN
cord-014597-66vd2mdu	731	16	a	a	DT
cord-014597-66vd2mdu	731	17	CHO	CHO	NNP
cord-014597-66vd2mdu	731	18	batch	batch	NN
cord-014597-66vd2mdu	731	19	process	process	NN
cord-014597-66vd2mdu	731	20	.	.	.
cord-014597-66vd2mdu	732	1	A	a	DT
cord-014597-66vd2mdu	732	2	mechanistic	mechanistic	JJ
cord-014597-66vd2mdu	732	3	model	model	NN
cord-014597-66vd2mdu	732	4	was	be	VBD
cord-014597-66vd2mdu	732	5	adapted	adapt	VBN
cord-014597-66vd2mdu	732	6	and	and	CC
cord-014597-66vd2mdu	732	7	modified	modify	VBN
cord-014597-66vd2mdu	732	8	from	from	IN
cord-014597-66vd2mdu	732	9	[	[	-LRB-
cord-014597-66vd2mdu	732	10	2	2	CD
cord-014597-66vd2mdu	732	11	]	]	-RRB-
cord-014597-66vd2mdu	732	12	and	and	CC
cord-014597-66vd2mdu	732	13	used	use	VBD
cord-014597-66vd2mdu	732	14	to	to	TO
cord-014597-66vd2mdu	732	15	describe	describe	VB
cord-014597-66vd2mdu	732	16	the	the	DT
cord-014597-66vd2mdu	732	17	dynamics	dynamic	NNS
cord-014597-66vd2mdu	732	18	of	of	IN
cord-014597-66vd2mdu	732	19	cell	cell	NN
cord-014597-66vd2mdu	732	20	metabolism	metabolism	NN
cord-014597-66vd2mdu	732	21	and	and	CC
cord-014597-66vd2mdu	732	22	antibody	antibody	NN
cord-014597-66vd2mdu	732	23	production	production	NN
cord-014597-66vd2mdu	732	24	of	of	IN
cord-014597-66vd2mdu	732	25	an	an	DT
cord-014597-66vd2mdu	732	26	IL-8	IL-8	NNP
cord-014597-66vd2mdu	732	27	antibody	antibody	NN
cord-014597-66vd2mdu	732	28	producing	produce	VBG
cord-014597-66vd2mdu	732	29	CHO	CHO	NNP
cord-014597-66vd2mdu	732	30	cell	cell	NN
cord-014597-66vd2mdu	732	31	line	line	NN
cord-014597-66vd2mdu	732	32	(	(	-LRB-
cord-014597-66vd2mdu	732	33	see	see	VB
cord-014597-66vd2mdu	732	34	abbreviation	abbreviation	NN
cord-014597-66vd2mdu	732	35	of	of	IN
cord-014597-66vd2mdu	732	36	Fig	Fig	NNP
cord-014597-66vd2mdu	732	37	.	.	.
cord-014597-66vd2mdu	732	38	1	1	CD
cord-014597-66vd2mdu	732	39	for	for	IN
cord-014597-66vd2mdu	732	40	cultivation	cultivation	NN
cord-014597-66vd2mdu	732	41	details	detail	NNS
cord-014597-66vd2mdu	732	42	)	)	-RRB-
cord-014597-66vd2mdu	732	43	.	.	.
cord-014597-66vd2mdu	733	1	Experiments	experiment	NNS
cord-014597-66vd2mdu	733	2	were	be	VBD
cord-014597-66vd2mdu	733	3	simulated	simulate	VBN
cord-014597-66vd2mdu	733	4	and	and	CC
cord-014597-66vd2mdu	733	5	compared	compare	VBN
cord-014597-66vd2mdu	733	6	to	to	IN
cord-014597-66vd2mdu	733	7	a	a	DT
cord-014597-66vd2mdu	733	8	fully	fully	RB
cord-014597-66vd2mdu	733	9	experimental	experimental	JJ
cord-014597-66vd2mdu	733	10	DoE.	DoE.	NNP
cord-014597-66vd2mdu	733	11	As	as	IN
cord-014597-66vd2mdu	733	12	can	can	MD
cord-014597-66vd2mdu	733	13	be	be	VB
cord-014597-66vd2mdu	733	14	seen	see	VBN
cord-014597-66vd2mdu	733	15	from	from	IN
cord-014597-66vd2mdu	733	16	Table	table	NN
cord-014597-66vd2mdu	733	17	1	1	CD
cord-014597-66vd2mdu	733	18	,	,	,
cord-014597-66vd2mdu	733	19	user	user	NN
cord-014597-66vd2mdu	733	20	defined	define	VBN
cord-014597-66vd2mdu	733	21	constraints	constraint	NNS
cord-014597-66vd2mdu	733	22	were	be	VBD
cord-014597-66vd2mdu	733	23	chosen	choose	VBN
cord-014597-66vd2mdu	733	24	to	to	TO
cord-014597-66vd2mdu	733	25	get	get	VB
cord-014597-66vd2mdu	733	26	a	a	DT
cord-014597-66vd2mdu	733	27	stable	stable	JJ
cord-014597-66vd2mdu	733	28	and	and	CC
cord-014597-66vd2mdu	733	29	reproducible	reproducible	JJ
cord-014597-66vd2mdu	733	30	process	process	NN
cord-014597-66vd2mdu	733	31	with	with	IN
cord-014597-66vd2mdu	733	32	the	the	DT
cord-014597-66vd2mdu	733	33	aim	aim	NN
cord-014597-66vd2mdu	733	34	of	of	IN
cord-014597-66vd2mdu	733	35	maximizing	maximize	VBG
cord-014597-66vd2mdu	733	36	the	the	DT
cord-014597-66vd2mdu	733	37	cell	cell	NN
cord-014597-66vd2mdu	733	38	density	density	NN
cord-014597-66vd2mdu	733	39	but	but	CC
cord-014597-66vd2mdu	733	40	decreased	decrease	VBD
cord-014597-66vd2mdu	733	41	lactate	lactate	NN
cord-014597-66vd2mdu	733	42	and	and	CC
cord-014597-66vd2mdu	733	43	ammonia	ammonia	NN
cord-014597-66vd2mdu	733	44	production	production	NN
cord-014597-66vd2mdu	733	45	.	.	.
cord-014597-66vd2mdu	734	1	At	at	IN
cord-014597-66vd2mdu	734	2	first	first	RB
cord-014597-66vd2mdu	734	3	,	,	,
cord-014597-66vd2mdu	734	4	the	the	DT
cord-014597-66vd2mdu	734	5	experimental	experimental	JJ
cord-014597-66vd2mdu	734	6	space	space	NN
cord-014597-66vd2mdu	734	7	was	be	VBD
cord-014597-66vd2mdu	734	8	estimated	estimate	VBN
cord-014597-66vd2mdu	734	9	by	by	IN
cord-014597-66vd2mdu	734	10	simulating	simulate	VBG
cord-014597-66vd2mdu	734	11	the	the	DT
cord-014597-66vd2mdu	734	12	responses	response	NNS
cord-014597-66vd2mdu	734	13	for	for	IN
cord-014597-66vd2mdu	734	14	broad	broad	JJ
cord-014597-66vd2mdu	734	15	concentration	concentration	NN
cord-014597-66vd2mdu	734	16	ranges	range	NNS
cord-014597-66vd2mdu	734	17	and	and	CC
cord-014597-66vd2mdu	734	18	calculating	calculate	VBG
cord-014597-66vd2mdu	734	19	the	the	DT
cord-014597-66vd2mdu	734	20	multiple	multiple	JJ
cord-014597-66vd2mdu	734	21	response	response	NN
cord-014597-66vd2mdu	734	22	desirability	desirability	NN
cord-014597-66vd2mdu	734	23	function	function	NN
cord-014597-66vd2mdu	734	24	(	(	-LRB-
cord-014597-66vd2mdu	734	25	Fig	Fig	NNP
cord-014597-66vd2mdu	734	26	.	.	.
cord-014597-66vd2mdu	734	27	1a	1a	CD
cord-014597-66vd2mdu	734	28	)	)	-RRB-
cord-014597-66vd2mdu	734	29	.	.	.
cord-014597-66vd2mdu	735	1	This	this	DT
cord-014597-66vd2mdu	735	2	results	result	VBZ
cord-014597-66vd2mdu	735	3	in	in	IN
cord-014597-66vd2mdu	735	4	a	a	DT
cord-014597-66vd2mdu	735	5	small	small	JJ
cord-014597-66vd2mdu	735	6	area	area	NN
cord-014597-66vd2mdu	735	7	(	(	-LRB-
cord-014597-66vd2mdu	735	8	turquoise	turquoise	NN
cord-014597-66vd2mdu	735	9	)	)	-RRB-
cord-014597-66vd2mdu	735	10	suggested	suggest	VBD
cord-014597-66vd2mdu	735	11	as	as	IN
cord-014597-66vd2mdu	735	12	experimental	experimental	JJ
cord-014597-66vd2mdu	735	13	space	space	NN
cord-014597-66vd2mdu	735	14	.	.	.
cord-014597-66vd2mdu	736	1	Experiments	experiment	NNS
cord-014597-66vd2mdu	736	2	were	be	VBD
cord-014597-66vd2mdu	736	3	planned	plan	VBN
cord-014597-66vd2mdu	736	4	within	within	IN
cord-014597-66vd2mdu	736	5	these	these	DT
cord-014597-66vd2mdu	736	6	boundaries	boundary	NNS
cord-014597-66vd2mdu	736	7	and	and	CC
cord-014597-66vd2mdu	736	8	responses	response	NNS
cord-014597-66vd2mdu	736	9	were	be	VBD
cord-014597-66vd2mdu	736	10	either	either	RB
cord-014597-66vd2mdu	736	11	simulated	simulate	VBN
cord-014597-66vd2mdu	737	1	(	(	-LRB-
cord-014597-66vd2mdu	737	2	Fig	Fig	NNP
cord-014597-66vd2mdu	737	3	.	.	.
cord-014597-66vd2mdu	737	4	1b	1b	CD
cord-014597-66vd2mdu	737	5	,	,	,
cord-014597-66vd2mdu	737	6	4	4	CD
cord-014597-66vd2mdu	737	7	cultivations	cultivation	NNS
cord-014597-66vd2mdu	737	8	for	for	IN
cord-014597-66vd2mdu	737	9	fitting	fit	VBG
cord-014597-66vd2mdu	737	10	the	the	DT
cord-014597-66vd2mdu	737	11	model	model	NN
cord-014597-66vd2mdu	737	12	)	)	-RRB-
cord-014597-66vd2mdu	737	13	or	or	CC
cord-014597-66vd2mdu	738	1	compared	compare	VBN
cord-014597-66vd2mdu	738	2	with	with	IN
cord-014597-66vd2mdu	738	3	the	the	DT
cord-014597-66vd2mdu	738	4	purely	purely	RB
cord-014597-66vd2mdu	738	5	experimental	experimental	JJ
cord-014597-66vd2mdu	738	6	responses	response	NNS
cord-014597-66vd2mdu	738	7	(	(	-LRB-
cord-014597-66vd2mdu	738	8	Fig	Fig	NNP
cord-014597-66vd2mdu	738	9	.	.	.
cord-014597-66vd2mdu	738	10	1c	1c	NNP
cord-014597-66vd2mdu	738	11	,	,	,
cord-014597-66vd2mdu	738	12	16	16	CD
cord-014597-66vd2mdu	738	13	cultivations	cultivation	NNS
cord-014597-66vd2mdu	738	14	)	)	-RRB-
cord-014597-66vd2mdu	738	15	.	.	.
cord-014597-66vd2mdu	739	1	Optimal	optimal	JJ
cord-014597-66vd2mdu	739	2	concentrations	concentration	NNS
cord-014597-66vd2mdu	739	3	for	for	IN
cord-014597-66vd2mdu	739	4	glutamine	glutamine	NN
cord-014597-66vd2mdu	739	5	and	and	CC
cord-014597-66vd2mdu	739	6	glucose	glucose	NN
cord-014597-66vd2mdu	739	7	with	with	IN
cord-014597-66vd2mdu	739	8	respect	respect	NN
cord-014597-66vd2mdu	739	9	to	to	IN
cord-014597-66vd2mdu	739	10	the	the	DT
cord-014597-66vd2mdu	739	11	constraints	constraint	NNS
cord-014597-66vd2mdu	739	12	are	be	VBP
cord-014597-66vd2mdu	739	13	in	in	IN
cord-014597-66vd2mdu	739	14	the	the	DT
cord-014597-66vd2mdu	739	15	lower	low	JJR
cord-014597-66vd2mdu	739	16	right	right	JJ
cord-014597-66vd2mdu	739	17	corner	corner	NN
cord-014597-66vd2mdu	739	18	and	and	CC
cord-014597-66vd2mdu	739	19	similar	similar	JJ
cord-014597-66vd2mdu	739	20	for	for	IN
cord-014597-66vd2mdu	739	21	both	both	DT
cord-014597-66vd2mdu	739	22	methods	method	NNS
cord-014597-66vd2mdu	739	23	(	(	-LRB-
cord-014597-66vd2mdu	739	24	red	red	JJ
cord-014597-66vd2mdu	739	25	frame	frame	NN
cord-014597-66vd2mdu	739	26	,	,	,
cord-014597-66vd2mdu	739	27	Fig	Fig	NNP
cord-014597-66vd2mdu	739	28	.	.	NNP
cord-014597-66vd2mdu	739	29	1	1	CD
cord-014597-66vd2mdu	739	30	)	)	-RRB-
cord-014597-66vd2mdu	739	31	.	.	.
cord-014597-66vd2mdu	740	1	Compared	compare	VBN
cord-014597-66vd2mdu	740	2	with	with	IN
cord-014597-66vd2mdu	740	3	the	the	DT
cord-014597-66vd2mdu	740	4	fully	fully	RB
cord-014597-66vd2mdu	740	5	experimental	experimental	JJ
cord-014597-66vd2mdu	740	6	design	design	NN
cord-014597-66vd2mdu	740	7	,	,	,
cord-014597-66vd2mdu	740	8	mDoE	mdoe	NN
cord-014597-66vd2mdu	740	9	results	result	NNS
cord-014597-66vd2mdu	740	10	in	in	IN
cord-014597-66vd2mdu	740	11	a	a	DT
cord-014597-66vd2mdu	740	12	reduction	reduction	NN
cord-014597-66vd2mdu	740	13	of	of	IN
cord-014597-66vd2mdu	740	14	75	75	CD
cord-014597-66vd2mdu	740	15	%	%	NN
cord-014597-66vd2mdu	740	16	in	in	IN
cord-014597-66vd2mdu	740	17	the	the	DT
cord-014597-66vd2mdu	740	18	number	number	NN
cord-014597-66vd2mdu	740	19	of	of	IN
cord-014597-66vd2mdu	740	20	experiments	experiment	NNS
cord-014597-66vd2mdu	740	21	(	(	-LRB-
cord-014597-66vd2mdu	740	22	4	4	CD
cord-014597-66vd2mdu	740	23	experiments	experiment	NNS
cord-014597-66vd2mdu	740	24	for	for	IN
cord-014597-66vd2mdu	740	25	modelling	model	VBG
cord-014597-66vd2mdu	740	26	vs.	vs.	CC
cord-014597-66vd2mdu	740	27	16	16	CD
cord-014597-66vd2mdu	740	28	experiments	experiment	NNS
cord-014597-66vd2mdu	740	29	in	in	IN
cord-014597-66vd2mdu	740	30	experimental	experimental	JJ
cord-014597-66vd2mdu	740	31	DoE	doe	NN
cord-014597-66vd2mdu	740	32	)	)	-RRB-
cord-014597-66vd2mdu	740	33	.	.	.
cord-014597-66vd2mdu	741	1	The	the	DT
cord-014597-66vd2mdu	741	2	method	method	NN
cord-014597-66vd2mdu	741	3	is	be	VBZ
cord-014597-66vd2mdu	741	4	intended	intend	VBN
cord-014597-66vd2mdu	741	5	to	to	TO
cord-014597-66vd2mdu	741	6	optimize	optimize	VB
cord-014597-66vd2mdu	741	7	cultivation	cultivation	NN
cord-014597-66vd2mdu	741	8	strategies	strategy	NNS
cord-014597-66vd2mdu	741	9	for	for	IN
cord-014597-66vd2mdu	741	10	mammalian	mammalian	JJ
cord-014597-66vd2mdu	741	11	cell	cell	NN
cord-014597-66vd2mdu	741	12	lines	line	NNS
cord-014597-66vd2mdu	741	13	and	and	CC
cord-014597-66vd2mdu	741	14	evaluated	evaluate	VBD
cord-014597-66vd2mdu	741	15	these	these	DT
cord-014597-66vd2mdu	741	16	before	before	IN
cord-014597-66vd2mdu	741	17	experiments	experiment	NNS
cord-014597-66vd2mdu	741	18	have	have	VBP
cord-014597-66vd2mdu	741	19	to	to	TO
cord-014597-66vd2mdu	741	20	be	be	VB
cord-014597-66vd2mdu	741	21	performed	perform	VBN
cord-014597-66vd2mdu	741	22	in	in	IN
cord-014597-66vd2mdu	741	23	laboratory	laboratory	NN
cord-014597-66vd2mdu	741	24	scale	scale	NN
cord-014597-66vd2mdu	741	25	.	.	.
cord-014597-66vd2mdu	742	1	This	this	DT
cord-014597-66vd2mdu	742	2	results	result	VBZ
cord-014597-66vd2mdu	742	3	in	in	IN
cord-014597-66vd2mdu	742	4	a	a	DT
cord-014597-66vd2mdu	742	5	significant	significant	JJ
cord-014597-66vd2mdu	742	6	time	time	NN
cord-014597-66vd2mdu	742	7	and	and	CC
cord-014597-66vd2mdu	742	8	cost	cost	NN
cord-014597-66vd2mdu	742	9	reduction	reduction	NN
cord-014597-66vd2mdu	742	10	during	during	IN
cord-014597-66vd2mdu	742	11	process	process	NN
cord-014597-66vd2mdu	742	12	development	development	NN
cord-014597-66vd2mdu	742	13	and	and	CC
cord-014597-66vd2mdu	742	14	process	process	NN
cord-014597-66vd2mdu	742	15	establishment	establishment	NN
cord-014597-66vd2mdu	742	16	.	.	.
cord-014597-66vd2mdu	743	1	The	the	DT
cord-014597-66vd2mdu	743	2	strategy	strategy	NN
cord-014597-66vd2mdu	743	3	is	be	VBZ
cord-014597-66vd2mdu	743	4	especially	especially	RB
cord-014597-66vd2mdu	743	5	intended	intend	VBN
cord-014597-66vd2mdu	743	6	for	for	IN
cord-014597-66vd2mdu	743	7	the	the	DT
cord-014597-66vd2mdu	743	8	use	use	NN
cord-014597-66vd2mdu	743	9	in	in	IN
cord-014597-66vd2mdu	743	10	multi	multi	JJ
cord-014597-66vd2mdu	743	11	-	-	JJ
cord-014597-66vd2mdu	743	12	single	single	JJ
cord-014597-66vd2mdu	743	13	-	-	HYPH
cord-014597-66vd2mdu	743	14	use	use	NN
cord-014597-66vd2mdu	743	15	-	-	HYPH
cord-014597-66vd2mdu	743	16	devices	device	NNS
cord-014597-66vd2mdu	743	17	to	to	TO
cord-014597-66vd2mdu	743	18	speed	speed	VB
cord-014597-66vd2mdu	743	19	up	up	RP
cord-014597-66vd2mdu	743	20	process	process	NN
cord-014597-66vd2mdu	743	21	development	development	NN
cord-014597-66vd2mdu	743	22	.	.	.
cord-014597-66vd2mdu	743	23	.	.	.
cord-014597-66vd2mdu	744	1	At	at	IN
cord-014597-66vd2mdu	744	2	a	a	DT
cord-014597-66vd2mdu	744	3	target	target	NN
cord-014597-66vd2mdu	744	4	cell	cell	NN
cord-014597-66vd2mdu	744	5	density	density	NN
cord-014597-66vd2mdu	744	6	of	of	IN
cord-014597-66vd2mdu	744	7	50	50	CD
cord-014597-66vd2mdu	744	8	*	*	NFP
cord-014597-66vd2mdu	744	9	10	10	CD
cord-014597-66vd2mdu	744	10	6	6	CD
cord-014597-66vd2mdu	744	11	vc	vc	NNP
cord-014597-66vd2mdu	744	12	/	/	SYM
cord-014597-66vd2mdu	744	13	mL	ml	NN
cord-014597-66vd2mdu	744	14	,	,	,
cord-014597-66vd2mdu	744	15	volumetric	volumetric	JJ
cord-014597-66vd2mdu	744	16	productivity	productivity	NN
cord-014597-66vd2mdu	744	17	was	be	VBD
cord-014597-66vd2mdu	744	18	stable	stable	JJ
cord-014597-66vd2mdu	744	19	for	for	IN
cord-014597-66vd2mdu	744	20	a	a	DT
cord-014597-66vd2mdu	744	21	30	30	CD
cord-014597-66vd2mdu	744	22	day	day	NN
cord-014597-66vd2mdu	744	23	steady	steady	JJ
cord-014597-66vd2mdu	744	24	state	state	NN
cord-014597-66vd2mdu	744	25	with	with	IN
cord-014597-66vd2mdu	744	26	EXCELL	EXCELL	NNP
cord-014597-66vd2mdu	745	1	®	®	,
cord-014597-66vd2mdu	745	2	Advanced	advanced	JJ
cord-014597-66vd2mdu	745	3	HD	HD	NNP
cord-014597-66vd2mdu	745	4	Perfusion	Perfusion	NNP
cord-014597-66vd2mdu	745	5	Medium	Medium	NNP
cord-014597-66vd2mdu	745	6	.	.	.
cord-014597-66vd2mdu	746	1	Shorter	short	JJR
cord-014597-66vd2mdu	746	2	steady	steady	JJ
cord-014597-66vd2mdu	746	3	states	state	NNS
cord-014597-66vd2mdu	746	4	were	be	VBD
cord-014597-66vd2mdu	746	5	tested	test	VBN
cord-014597-66vd2mdu	746	6	at	at	IN
cord-014597-66vd2mdu	746	7	80	80	CD
cord-014597-66vd2mdu	746	8	*	*	SYM
cord-014597-66vd2mdu	746	9	10	10	CD
cord-014597-66vd2mdu	746	10	6	6	CD
cord-014597-66vd2mdu	746	11	vc	vc	NN
cord-014597-66vd2mdu	746	12	/	/	SYM
cord-014597-66vd2mdu	746	13	mL	ml	NN
cord-014597-66vd2mdu	746	14	Background	background	NN
cord-014597-66vd2mdu	746	15	For	for	IN
cord-014597-66vd2mdu	746	16	the	the	DT
cord-014597-66vd2mdu	746	17	large	large	JJ
cord-014597-66vd2mdu	746	18	-	-	HYPH
cord-014597-66vd2mdu	746	19	scale	scale	NN
cord-014597-66vd2mdu	746	20	production	production	NN
cord-014597-66vd2mdu	746	21	of	of	IN
cord-014597-66vd2mdu	746	22	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	746	23	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	746	24	,	,	,
cord-014597-66vd2mdu	746	25	fedbatch	fedbatch	NN
cord-014597-66vd2mdu	746	26	culture	culture	NN
cord-014597-66vd2mdu	746	27	has	have	VBZ
cord-014597-66vd2mdu	746	28	been	be	VBN
cord-014597-66vd2mdu	746	29	widely	widely	RB
cord-014597-66vd2mdu	746	30	used	use	VBN
cord-014597-66vd2mdu	746	31	for	for	IN
cord-014597-66vd2mdu	746	32	its	-PRON-	PRP$
cord-014597-66vd2mdu	746	33	operational	operational	JJ
cord-014597-66vd2mdu	746	34	simplicity	simplicity	NN
cord-014597-66vd2mdu	746	35	and	and	CC
cord-014597-66vd2mdu	746	36	high	high	JJ
cord-014597-66vd2mdu	746	37	titer	titer	NN
cord-014597-66vd2mdu	746	38	.	.	.
cord-014597-66vd2mdu	747	1	However	however	RB
cord-014597-66vd2mdu	747	2	,	,	,
cord-014597-66vd2mdu	747	3	repeated	repeat	VBN
cord-014597-66vd2mdu	747	4	feeding	feeding	NN
cord-014597-66vd2mdu	747	5	of	of	IN
cord-014597-66vd2mdu	747	6	medium	medium	NN
cord-014597-66vd2mdu	747	7	concentrates	concentrate	VBZ
cord-014597-66vd2mdu	747	8	and/	and/	CD
cord-014597-66vd2mdu	747	9	or	or	CC
cord-014597-66vd2mdu	747	10	addition	addition	NN
cord-014597-66vd2mdu	747	11	of	of	IN
cord-014597-66vd2mdu	747	12	a	a	DT
cord-014597-66vd2mdu	747	13	base	base	NN
cord-014597-66vd2mdu	747	14	to	to	TO
cord-014597-66vd2mdu	747	15	maintain	maintain	VB
cord-014597-66vd2mdu	747	16	optimal	optimal	JJ
cord-014597-66vd2mdu	748	1	pH	pH	NNP
cord-014597-66vd2mdu	748	2	during	during	IN
cord-014597-66vd2mdu	748	3	fed	fed	NNP
cord-014597-66vd2mdu	748	4	-	-	HYPH
cord-014597-66vd2mdu	748	5	batch	batch	NN
cord-014597-66vd2mdu	748	6	culture	culture	NN
cord-014597-66vd2mdu	748	7	lead	lead	VBP
cord-014597-66vd2mdu	748	8	to	to	TO
cord-014597-66vd2mdu	748	9	increase	increase	VB
cord-014597-66vd2mdu	748	10	in	in	IN
cord-014597-66vd2mdu	748	11	osmolality	osmolality	NN
cord-014597-66vd2mdu	748	12	.	.	.
cord-014597-66vd2mdu	749	1	The	the	DT
cord-014597-66vd2mdu	749	2	hyperosmolality	hyperosmolality	NN
cord-014597-66vd2mdu	749	3	affects	affect	VBZ
cord-014597-66vd2mdu	749	4	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	749	5	in	in	IN
cord-014597-66vd2mdu	749	6	a	a	DT
cord-014597-66vd2mdu	749	7	protein	protein	NN
cord-014597-66vd2mdu	749	8	-	-	HYPH
cord-014597-66vd2mdu	749	9	specific	specific	JJ
cord-014597-66vd2mdu	749	10	manner	manner	NN
cord-014597-66vd2mdu	749	11	.	.	.
cord-014597-66vd2mdu	750	1	However	however	RB
cord-014597-66vd2mdu	750	2	,	,	,
cord-014597-66vd2mdu	750	3	the	the	DT
cord-014597-66vd2mdu	750	4	mechanism	mechanism	NN
cord-014597-66vd2mdu	750	5	behind	behind	IN
cord-014597-66vd2mdu	750	6	such	such	JJ
cord-014597-66vd2mdu	750	7	osmolality	osmolality	NN
cord-014597-66vd2mdu	750	8	-	-	HYPH
cord-014597-66vd2mdu	750	9	dependent	dependent	JJ
cord-014597-66vd2mdu	750	10	variations	variation	NNS
cord-014597-66vd2mdu	750	11	in	in	IN
cord-014597-66vd2mdu	750	12	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	750	13	in	in	IN
cord-014597-66vd2mdu	750	14	recombinant	recombinant	JJ
cord-014597-66vd2mdu	750	15	Chinese	chinese	JJ
cord-014597-66vd2mdu	750	16	hamster	hamster	NN
cord-014597-66vd2mdu	750	17	ovary	ovary	NN
cord-014597-66vd2mdu	750	18	(	(	-LRB-
cord-014597-66vd2mdu	750	19	rCHO	rCHO	NNP
cord-014597-66vd2mdu	750	20	)	)	-RRB-
cord-014597-66vd2mdu	750	21	cells	cell	NNS
cord-014597-66vd2mdu	750	22	remains	remain	VBZ
cord-014597-66vd2mdu	750	23	unclear	unclear	JJ
cord-014597-66vd2mdu	750	24	.	.	.
cord-014597-66vd2mdu	751	1	In	in	IN
cord-014597-66vd2mdu	751	2	this	this	DT
cord-014597-66vd2mdu	751	3	study	study	NN
cord-014597-66vd2mdu	751	4	,	,	,
cord-014597-66vd2mdu	751	5	to	to	TO
cord-014597-66vd2mdu	751	6	better	better	RB
cord-014597-66vd2mdu	751	7	understand	understand	VB
cord-014597-66vd2mdu	751	8	the	the	DT
cord-014597-66vd2mdu	751	9	effect	effect	NN
cord-014597-66vd2mdu	751	10	of	of	IN
cord-014597-66vd2mdu	751	11	hyperosmolality	hyperosmolality	NN
cord-014597-66vd2mdu	751	12	on	on	IN
cord-014597-66vd2mdu	751	13	the	the	DT
cord-014597-66vd2mdu	751	14	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	751	15	of	of	IN
cord-014597-66vd2mdu	751	16	a	a	DT
cord-014597-66vd2mdu	751	17	protein	protein	NN
cord-014597-66vd2mdu	751	18	produced	produce	VBN
cord-014597-66vd2mdu	751	19	from	from	IN
cord-014597-66vd2mdu	751	20	rCHO	rcho	JJ
cord-014597-66vd2mdu	751	21	cells	cell	NNS
cord-014597-66vd2mdu	751	22	,	,	,
cord-014597-66vd2mdu	751	23	we	-PRON-	PRP
cord-014597-66vd2mdu	751	24	investigated	investigate	VBD
cord-014597-66vd2mdu	751	25	52	52	CD
cord-014597-66vd2mdu	751	26	N	n	CD
cord-014597-66vd2mdu	751	27	-	-	HYPH
cord-014597-66vd2mdu	751	28	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	751	29	-	-	HYPH
cord-014597-66vd2mdu	751	30	related	relate	VBN
cord-014597-66vd2mdu	751	31	gene	gene	NN
cord-014597-66vd2mdu	751	32	expression	expression	NN
cord-014597-66vd2mdu	751	33	and	and	CC
cord-014597-66vd2mdu	751	34	N	n	NN
cord-014597-66vd2mdu	751	35	-	-	HYPH
cord-014597-66vd2mdu	751	36	linked	link	VBN
cord-014597-66vd2mdu	751	37	glycan	glycan	NN
cord-014597-66vd2mdu	751	38	structure	structure	NN
cord-014597-66vd2mdu	751	39	in	in	IN
cord-014597-66vd2mdu	751	40	Fc	Fc	NNP
cord-014597-66vd2mdu	751	41	-	-	HYPH
cord-014597-66vd2mdu	751	42	fusion	fusion	NN
cord-014597-66vd2mdu	751	43	protein	protein	NN
cord-014597-66vd2mdu	751	44	-	-	HYPH
cord-014597-66vd2mdu	751	45	producing	produce	VBG
cord-014597-66vd2mdu	751	46	rCHO	rcho	NN
cord-014597-66vd2mdu	751	47	cells	cell	NNS
cord-014597-66vd2mdu	751	48	exposed	expose	VBN
cord-014597-66vd2mdu	751	49	to	to	IN
cord-014597-66vd2mdu	751	50	hyperosmotic	hyperosmotic	JJ
cord-014597-66vd2mdu	751	51	conditions	condition	NNS
cord-014597-66vd2mdu	751	52	.	.	.
cord-014597-66vd2mdu	752	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	752	2	,	,	,
cord-014597-66vd2mdu	752	3	to	to	TO
cord-014597-66vd2mdu	752	4	validate	validate	VB
cord-014597-66vd2mdu	752	5	the	the	DT
cord-014597-66vd2mdu	752	6	effect	effect	NN
cord-014597-66vd2mdu	752	7	of	of	IN
cord-014597-66vd2mdu	752	8	hyperosmolality	hyperosmolality	NN
cord-014597-66vd2mdu	752	9	on	on	IN
cord-014597-66vd2mdu	752	10	protein	protein	NN
cord-014597-66vd2mdu	752	11	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	752	12	,	,	,
cord-014597-66vd2mdu	752	13	we	-PRON-	PRP
cord-014597-66vd2mdu	752	14	performed	perform	VBD
cord-014597-66vd2mdu	752	15	hyperosmotic	hyperosmotic	JJ
cord-014597-66vd2mdu	752	16	culture	culture	NN
cord-014597-66vd2mdu	752	17	supplemented	supplement	VBN
cord-014597-66vd2mdu	752	18	with	with	IN
cord-014597-66vd2mdu	752	19	betaine	betaine	NN
cord-014597-66vd2mdu	752	20	,	,	,
cord-014597-66vd2mdu	752	21	an	an	DT
cord-014597-66vd2mdu	752	22	osmoprotectant	osmoprotectant	NN
cord-014597-66vd2mdu	752	23	,	,	,
cord-014597-66vd2mdu	752	24	and	and	CC
cord-014597-66vd2mdu	752	25	then	then	RB
cord-014597-66vd2mdu	752	26	analyzed	analyze	VBD
cord-014597-66vd2mdu	752	27	the	the	DT
cord-014597-66vd2mdu	752	28	N	N	NNP
cord-014597-66vd2mdu	752	29	-	-	HYPH
cord-014597-66vd2mdu	752	30	linked	link	VBN
cord-014597-66vd2mdu	752	31	glycan	glycan	NN
cord-014597-66vd2mdu	752	32	structure	structure	NN
cord-014597-66vd2mdu	752	33	and	and	CC
cord-014597-66vd2mdu	752	34	mRNA	mrna	NN
cord-014597-66vd2mdu	752	35	levels	level	NNS
cord-014597-66vd2mdu	752	36	of	of	IN
cord-014597-66vd2mdu	752	37	N	n	CD
cord-014597-66vd2mdu	752	38	-	-	HYPH
cord-014597-66vd2mdu	752	39	glycan	glycan	NN
cord-014597-66vd2mdu	752	40	branching	branch	VBG
cord-014597-66vd2mdu	752	41	/	/	SYM
cord-014597-66vd2mdu	752	42	antennary	antennary	JJ
cord-014597-66vd2mdu	752	43	genes	gene	NNS
cord-014597-66vd2mdu	752	44	.	.	.
cord-014597-66vd2mdu	753	1	After	after	IN
cord-014597-66vd2mdu	753	2	three	three	CD
cord-014597-66vd2mdu	753	3	days	day	NNS
cord-014597-66vd2mdu	753	4	of	of	IN
cord-014597-66vd2mdu	753	5	hyperosmotic	hyperosmotic	JJ
cord-014597-66vd2mdu	753	6	culture	culture	NN
cord-014597-66vd2mdu	753	7	,	,	,
cord-014597-66vd2mdu	753	8	nine	nine	CD
cord-014597-66vd2mdu	753	9	genes	gene	NNS
cord-014597-66vd2mdu	753	10	(	(	-LRB-
cord-014597-66vd2mdu	753	11	ugp	ugp	JJ
cord-014597-66vd2mdu	753	12	,	,	,
cord-014597-66vd2mdu	753	13	slc35a3	slc35a3	NNP
cord-014597-66vd2mdu	753	14	,	,	,
cord-014597-66vd2mdu	753	15	slc35d2	slc35d2	NNP
cord-014597-66vd2mdu	753	16	,	,	,
cord-014597-66vd2mdu	753	17	gcs1	gcs1	UH
cord-014597-66vd2mdu	753	18	,	,	,
cord-014597-66vd2mdu	753	19	manea	manea	NNP
cord-014597-66vd2mdu	753	20	,	,	,
cord-014597-66vd2mdu	753	21	mgat2	mgat2	NN
cord-014597-66vd2mdu	753	22	,	,	,
cord-014597-66vd2mdu	753	23	mgat5b	mgat5b	NNP
cord-014597-66vd2mdu	753	24	,	,	,
cord-014597-66vd2mdu	753	25	b4galt3	b4galt3	JJ
cord-014597-66vd2mdu	753	26	,	,	,
cord-014597-66vd2mdu	753	27	and	and	CC
cord-014597-66vd2mdu	753	28	b4galt4	b4galt4	LS
cord-014597-66vd2mdu	753	29	)	)	-RRB-
cord-014597-66vd2mdu	753	30	were	be	VBD
cord-014597-66vd2mdu	753	31	differentially	differentially	RB
cord-014597-66vd2mdu	753	32	expressed	express	VBN
cord-014597-66vd2mdu	753	33	over	over	IN
cord-014597-66vd2mdu	753	34	1.5-fold	1.5-fold	CD
cord-014597-66vd2mdu	753	35	of	of	IN
cord-014597-66vd2mdu	753	36	the	the	DT
cord-014597-66vd2mdu	753	37	control	control	NN
cord-014597-66vd2mdu	753	38	,	,	,
cord-014597-66vd2mdu	753	39	and	and	CC
cord-014597-66vd2mdu	753	40	all	all	PDT
cord-014597-66vd2mdu	753	41	these	these	DT
cord-014597-66vd2mdu	753	42	genes	gene	NNS
cord-014597-66vd2mdu	753	43	were	be	VBD
cord-014597-66vd2mdu	753	44	down	down	RB
cord-014597-66vd2mdu	753	45	-	-	HYPH
cord-014597-66vd2mdu	753	46	regulated	regulate	VBN
cord-014597-66vd2mdu	753	47	.	.	.
cord-014597-66vd2mdu	754	1	N	n	CD
cord-014597-66vd2mdu	754	2	-	-	HYPH
cord-014597-66vd2mdu	754	3	linked	link	VBN
cord-014597-66vd2mdu	754	4	glycan	glycan	NN
cord-014597-66vd2mdu	754	5	analysis	analysis	NN
cord-014597-66vd2mdu	754	6	by	by	IN
cord-014597-66vd2mdu	754	7	anion	anion	NN
cord-014597-66vd2mdu	754	8	exchange	exchange	NN
cord-014597-66vd2mdu	754	9	and	and	CC
cord-014597-66vd2mdu	754	10	hydrophilic	hydrophilic	JJ
cord-014597-66vd2mdu	754	11	interaction	interaction	NN
cord-014597-66vd2mdu	754	12	HPLC	HPLC	NNP
cord-014597-66vd2mdu	754	13	showed	show	VBD
cord-014597-66vd2mdu	754	14	that	that	IN
cord-014597-66vd2mdu	754	15	the	the	DT
cord-014597-66vd2mdu	754	16	proportion	proportion	NN
cord-014597-66vd2mdu	754	17	of	of	IN
cord-014597-66vd2mdu	754	18	highly	highly	RB
cord-014597-66vd2mdu	754	19	sialylated	sialylate	VBN
cord-014597-66vd2mdu	754	20	(	(	-LRB-
cord-014597-66vd2mdu	754	21	di-	di-	XX
cord-014597-66vd2mdu	754	22	,	,	,
cord-014597-66vd2mdu	754	23	tri-	tri-	XX
cord-014597-66vd2mdu	754	24	,	,	,
cord-014597-66vd2mdu	754	25	tetra-	tetra-	NN
cord-014597-66vd2mdu	754	26	)	)	-RRB-
cord-014597-66vd2mdu	754	27	and	and	CC
cord-014597-66vd2mdu	754	28	tetra	tetra	NNP
cord-014597-66vd2mdu	754	29	-	-	HYPH
cord-014597-66vd2mdu	754	30	antennary	antennary	NNP
cord-014597-66vd2mdu	754	31	N	N	NNP
cord-014597-66vd2mdu	754	32	-	-	HYPH
cord-014597-66vd2mdu	754	33	linked	link	VBN
cord-014597-66vd2mdu	754	34	glycans	glycan	NNS
cord-014597-66vd2mdu	754	35	was	be	VBD
cord-014597-66vd2mdu	754	36	significantly	significantly	RB
cord-014597-66vd2mdu	754	37	decreased	decrease	VBN
cord-014597-66vd2mdu	754	38	upon	upon	IN
cord-014597-66vd2mdu	754	39	hyperosmotic	hyperosmotic	JJ
cord-014597-66vd2mdu	754	40	culture	culture	NN
cord-014597-66vd2mdu	754	41	.	.	.
cord-014597-66vd2mdu	755	1	Addition	addition	NN
cord-014597-66vd2mdu	755	2	of	of	IN
cord-014597-66vd2mdu	755	3	betaine	betaine	NN
cord-014597-66vd2mdu	755	4	,	,	,
cord-014597-66vd2mdu	755	5	an	an	DT
cord-014597-66vd2mdu	755	6	osmoprotectant	osmoprotectant	NN
cord-014597-66vd2mdu	755	7	,	,	,
cord-014597-66vd2mdu	755	8	to	to	IN
cord-014597-66vd2mdu	755	9	the	the	DT
cord-014597-66vd2mdu	755	10	hyperosmotic	hyperosmotic	JJ
cord-014597-66vd2mdu	755	11	culture	culture	NN
cord-014597-66vd2mdu	755	12	significantly	significantly	RB
cord-014597-66vd2mdu	755	13	increased	increase	VBD
cord-014597-66vd2mdu	755	14	the	the	DT
cord-014597-66vd2mdu	755	15	proportion	proportion	NN
cord-014597-66vd2mdu	755	16	of	of	IN
cord-014597-66vd2mdu	755	17	highly	highly	RB
cord-014597-66vd2mdu	755	18	sialylated	sialylate	VBN
cord-014597-66vd2mdu	755	19	and	and	CC
cord-014597-66vd2mdu	755	20	tetra	tetra	NNP
cord-014597-66vd2mdu	755	21	-	-	HYPH
cord-014597-66vd2mdu	755	22	antennary	antennary	NNP
cord-014597-66vd2mdu	755	23	N	N	NNP
cord-014597-66vd2mdu	755	24	-	-	HYPH
cord-014597-66vd2mdu	755	25	linked	link	VBN
cord-014597-66vd2mdu	755	26	glycans	glycan	NNS
cord-014597-66vd2mdu	755	27	(	(	-LRB-
cord-014597-66vd2mdu	755	28	P	p	NN
cord-014597-66vd2mdu	755	29	≤	≤	NN
cord-014597-66vd2mdu	755	30	0.05	0.05	CD
cord-014597-66vd2mdu	755	31	)	)	-RRB-
cord-014597-66vd2mdu	755	32	,	,	,
cord-014597-66vd2mdu	755	33	while	while	IN
cord-014597-66vd2mdu	755	34	it	-PRON-	PRP
cord-014597-66vd2mdu	755	35	increased	increase	VBD
cord-014597-66vd2mdu	755	36	the	the	DT
cord-014597-66vd2mdu	755	37	expression	expression	NN
cord-014597-66vd2mdu	755	38	of	of	IN
cord-014597-66vd2mdu	755	39	the	the	DT
cord-014597-66vd2mdu	755	40	N	n	CD
cord-014597-66vd2mdu	755	41	-	-	HYPH
cord-014597-66vd2mdu	755	42	glycan	glycan	NN
cord-014597-66vd2mdu	755	43	branching	branch	VBG
cord-014597-66vd2mdu	755	44	/	/	SYM
cord-014597-66vd2mdu	755	45	antennary	antennary	JJ
cord-014597-66vd2mdu	755	46	genes	gene	NNS
cord-014597-66vd2mdu	755	47	(	(	-LRB-
cord-014597-66vd2mdu	755	48	mgat2	mgat2	NNP
cord-014597-66vd2mdu	755	49	and	and	CC
cord-014597-66vd2mdu	755	50	mgat4b	mgat4b	NNP
cord-014597-66vd2mdu	755	51	)	)	-RRB-
cord-014597-66vd2mdu	755	52	.	.	.
cord-014597-66vd2mdu	756	1	Thus	thus	RB
cord-014597-66vd2mdu	756	2	,	,	,
cord-014597-66vd2mdu	756	3	decreased	decrease	VBD
cord-014597-66vd2mdu	756	4	expression	expression	NN
cord-014597-66vd2mdu	756	5	of	of	IN
cord-014597-66vd2mdu	756	6	the	the	DT
cord-014597-66vd2mdu	756	7	genes	gene	NNS
cord-014597-66vd2mdu	756	8	with	with	IN
cord-014597-66vd2mdu	756	9	roles	role	NNS
cord-014597-66vd2mdu	756	10	in	in	IN
cord-014597-66vd2mdu	756	11	the	the	DT
cord-014597-66vd2mdu	756	12	N	n	CD
cord-014597-66vd2mdu	756	13	-	-	HYPH
cord-014597-66vd2mdu	756	14	glycan	glycan	NN
cord-014597-66vd2mdu	756	15	biosynthesis	biosynthesis	NN
cord-014597-66vd2mdu	756	16	pathway	pathway	NN
cord-014597-66vd2mdu	756	17	correlated	correlate	VBN
cord-014597-66vd2mdu	756	18	with	with	IN
cord-014597-66vd2mdu	756	19	reduced	reduce	VBN
cord-014597-66vd2mdu	756	20	sialic	sialic	NN
cord-014597-66vd2mdu	756	21	acid	acid	NN
cord-014597-66vd2mdu	756	22	content	content	NN
cord-014597-66vd2mdu	756	23	of	of	IN
cord-014597-66vd2mdu	756	24	Fc	Fc	NNP
cord-014597-66vd2mdu	756	25	-	-	HYPH
cord-014597-66vd2mdu	756	26	fusion	fusion	NN
cord-014597-66vd2mdu	756	27	protein	protein	NN
cord-014597-66vd2mdu	756	28	caused	cause	VBN
cord-014597-66vd2mdu	756	29	by	by	IN
cord-014597-66vd2mdu	756	30	hyperosmolar	hyperosmolar	JJ
cord-014597-66vd2mdu	756	31	conditions	condition	NNS
cord-014597-66vd2mdu	756	32	.	.	.
cord-014597-66vd2mdu	757	1	Conclusions	conclusion	NNS
cord-014597-66vd2mdu	757	2	Taken	take	VBN
cord-014597-66vd2mdu	757	3	together	together	RB
cord-014597-66vd2mdu	757	4	,	,	,
cord-014597-66vd2mdu	757	5	the	the	DT
cord-014597-66vd2mdu	757	6	results	result	NNS
cord-014597-66vd2mdu	757	7	obtained	obtain	VBN
cord-014597-66vd2mdu	757	8	in	in	IN
cord-014597-66vd2mdu	757	9	this	this	DT
cord-014597-66vd2mdu	757	10	study	study	NN
cord-014597-66vd2mdu	757	11	provide	provide	VB
cord-014597-66vd2mdu	757	12	a	a	DT
cord-014597-66vd2mdu	757	13	better	well	JJR
cord-014597-66vd2mdu	757	14	understanding	understanding	NN
cord-014597-66vd2mdu	757	15	of	of	IN
cord-014597-66vd2mdu	757	16	the	the	DT
cord-014597-66vd2mdu	757	17	detrimental	detrimental	JJ
cord-014597-66vd2mdu	757	18	effects	effect	NNS
cord-014597-66vd2mdu	757	19	of	of	IN
cord-014597-66vd2mdu	757	20	hyperosmolality	hyperosmolality	NN
cord-014597-66vd2mdu	757	21	on	on	IN
cord-014597-66vd2mdu	757	22	N	n	NN
cord-014597-66vd2mdu	757	23	-	-	HYPH
cord-014597-66vd2mdu	757	24	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	757	25	,	,	,
cord-014597-66vd2mdu	757	26	especially	especially	RB
cord-014597-66vd2mdu	757	27	sialylation	sialylation	NN
cord-014597-66vd2mdu	757	28	,	,	,
cord-014597-66vd2mdu	757	29	in	in	IN
cord-014597-66vd2mdu	757	30	rCHO	rcho	NN
cord-014597-66vd2mdu	757	31	cells	cell	NNS
cord-014597-66vd2mdu	757	32	.	.	.
cord-014597-66vd2mdu	758	1	The	the	DT
cord-014597-66vd2mdu	758	2	identified	identify	VBN
cord-014597-66vd2mdu	758	3	genes	gene	NNS
cord-014597-66vd2mdu	758	4	,	,	,
cord-014597-66vd2mdu	758	5	particularly	particularly	RB
cord-014597-66vd2mdu	758	6	mgat2	mgat2	NN
cord-014597-66vd2mdu	758	7	and	and	CC
cord-014597-66vd2mdu	758	8	mgat4b	mgat4b	NNP
cord-014597-66vd2mdu	758	9	,	,	,
cord-014597-66vd2mdu	758	10	are	be	VBP
cord-014597-66vd2mdu	758	11	potential	potential	JJ
cord-014597-66vd2mdu	758	12	targets	target	NNS
cord-014597-66vd2mdu	758	13	for	for	IN
cord-014597-66vd2mdu	758	14	engineering	engineering	NN
cord-014597-66vd2mdu	758	15	in	in	IN
cord-014597-66vd2mdu	758	16	CHO	CHO	NNP
cord-014597-66vd2mdu	758	17	cells	cell	NNS
cord-014597-66vd2mdu	758	18	to	to	TO
cord-014597-66vd2mdu	758	19	overcome	overcome	VB
cord-014597-66vd2mdu	758	20	the	the	DT
cord-014597-66vd2mdu	758	21	impact	impact	NN
cord-014597-66vd2mdu	758	22	of	of	IN
cord-014597-66vd2mdu	758	23	hyperosmolality	hyperosmolality	NN
cord-014597-66vd2mdu	758	24	on	on	IN
cord-014597-66vd2mdu	758	25	glycoprotein	glycoprotein	NN
cord-014597-66vd2mdu	758	26	sialylation	sialylation	NN
cord-014597-66vd2mdu	758	27	.	.	.
cord-014597-66vd2mdu	759	1	Disruptive	disruptive	JJ
cord-014597-66vd2mdu	759	2	cost	cost	NN
cord-014597-66vd2mdu	759	3	-	-	HYPH
cord-014597-66vd2mdu	759	4	effective	effective	JJ
cord-014597-66vd2mdu	759	5	antibody	antibody	NN
cord-014597-66vd2mdu	759	6	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	759	7	platform	platform	NN
cord-014597-66vd2mdu	759	8	based	base	VBN
cord-014597-66vd2mdu	759	9	on	on	IN
cord-014597-66vd2mdu	759	10	cutting	cut	VBG
cord-014597-66vd2mdu	759	11	-	-	HYPH
cord-014597-66vd2mdu	759	12	edge	edge	NN
cord-014597-66vd2mdu	759	13	purification	purification	NN
cord-014597-66vd2mdu	759	14	process	process	NN
cord-014597-66vd2mdu	759	15	V.	V.	NNP
cord-014597-66vd2mdu	759	16	Medvedev	Medvedev	NNP
cord-014597-66vd2mdu	759	17	,	,	,
cord-014597-66vd2mdu	759	18	M.	M.	NNP
cord-014597-66vd2mdu	759	19	Duyck	Duyck	NNP
cord-014597-66vd2mdu	759	20	,	,	,
cord-014597-66vd2mdu	759	21	T.	T.	NNP
cord-014597-66vd2mdu	759	22	Albano	Albano	NNP
cord-014597-66vd2mdu	759	23	,	,	,
cord-014597-66vd2mdu	759	24	J.	J.	NNP
cord-014597-66vd2mdu	759	25	Castillo	Castillo	NNP
cord-014597-66vd2mdu	759	26	Univercells	Univercells	NNP
cord-014597-66vd2mdu	759	27	SA	SA	NNP
cord-014597-66vd2mdu	759	28	,	,	,
cord-014597-66vd2mdu	759	29	Gosselies	Gosselies	NNP
cord-014597-66vd2mdu	759	30	,	,	,
cord-014597-66vd2mdu	759	31	Belgium	Belgium	NNP
cord-014597-66vd2mdu	759	32	Correspondence	Correspondence	NNP
cord-014597-66vd2mdu	759	33	:	:	:
cord-014597-66vd2mdu	759	34	V.	V.	NNP
cord-014597-66vd2mdu	759	35	Medvedev	Medvedev	NNP
cord-014597-66vd2mdu	759	36	(	(	-LRB-
cord-014597-66vd2mdu	759	37	v.medvedev@univercells.com	v.medvedev@univercells.com	ADD
cord-014597-66vd2mdu	759	38	)	)	-RRB-
cord-014597-66vd2mdu	760	1	BMC	BMC	NNP
cord-014597-66vd2mdu	760	2	Proceedings	Proceedings	NNP
cord-014597-66vd2mdu	760	3	2018	2018	CD
cord-014597-66vd2mdu	760	4	,	,	,
cord-014597-66vd2mdu	761	1	12(Suppl	12(suppl	CD
cord-014597-66vd2mdu	762	1	1):P-093	1):p-093	CD
cord-014597-66vd2mdu	763	1	Background	background	NN
cord-014597-66vd2mdu	763	2	Demand	demand	NN
cord-014597-66vd2mdu	763	3	for	for	IN
cord-014597-66vd2mdu	763	4	high	high	JJ
cord-014597-66vd2mdu	763	5	-	-	HYPH
cord-014597-66vd2mdu	763	6	quality	quality	NN
cord-014597-66vd2mdu	763	7	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	763	8	antibodies	antibody	NNS
cord-014597-66vd2mdu	763	9	is	be	VBZ
cord-014597-66vd2mdu	763	10	growing	grow	VBG
cord-014597-66vd2mdu	763	11	exponentially	exponentially	RB
cord-014597-66vd2mdu	763	12	,	,	,
cord-014597-66vd2mdu	763	13	calling	call	VBG
cord-014597-66vd2mdu	763	14	for	for	IN
cord-014597-66vd2mdu	763	15	new	new	JJ
cord-014597-66vd2mdu	763	16	production	production	NN
cord-014597-66vd2mdu	763	17	capacities	capacity	NNS
cord-014597-66vd2mdu	763	18	.	.	.
cord-014597-66vd2mdu	764	1	Overcoming	overcome	VBG
cord-014597-66vd2mdu	764	2	current	current	JJ
cord-014597-66vd2mdu	764	3	limitations	limitation	NNS
cord-014597-66vd2mdu	764	4	of	of	IN
cord-014597-66vd2mdu	764	5	conventional	conventional	JJ
cord-014597-66vd2mdu	764	6	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	764	7	strategies	strategy	NNS
cord-014597-66vd2mdu	764	8	,	,	,
cord-014597-66vd2mdu	764	9	namely	namely	RB
cord-014597-66vd2mdu	764	10	the	the	DT
cord-014597-66vd2mdu	764	11	high	high	JJ
cord-014597-66vd2mdu	764	12	capital	capital	NN
cord-014597-66vd2mdu	764	13	investment	investment	NN
cord-014597-66vd2mdu	764	14	and	and	CC
cord-014597-66vd2mdu	764	15	production	production	NN
cord-014597-66vd2mdu	764	16	cost	cost	NN
cord-014597-66vd2mdu	764	17	,	,	,
cord-014597-66vd2mdu	764	18	can	can	MD
cord-014597-66vd2mdu	764	19	only	only	RB
cord-014597-66vd2mdu	764	20	be	be	VB
cord-014597-66vd2mdu	764	21	achieved	achieve	VBN
cord-014597-66vd2mdu	764	22	through	through	IN
cord-014597-66vd2mdu	764	23	innovative	innovative	JJ
cord-014597-66vd2mdu	764	24	process	process	NN
cord-014597-66vd2mdu	764	25	designs	design	NNS
cord-014597-66vd2mdu	764	26	based	base	VBN
cord-014597-66vd2mdu	764	27	on	on	IN
cord-014597-66vd2mdu	764	28	the	the	DT
cord-014597-66vd2mdu	764	29	latest	late	JJS
cord-014597-66vd2mdu	764	30	technologies	technology	NNS
cord-014597-66vd2mdu	764	31	.	.	.
cord-014597-66vd2mdu	765	1	This	this	DT
cord-014597-66vd2mdu	765	2	study	study	NN
cord-014597-66vd2mdu	765	3	presents	present	VBZ
cord-014597-66vd2mdu	765	4	a	a	DT
cord-014597-66vd2mdu	765	5	process	process	NN
cord-014597-66vd2mdu	765	6	design	design	NN
cord-014597-66vd2mdu	765	7	combining	combine	VBG
cord-014597-66vd2mdu	765	8	batch	batch	NN
cord-014597-66vd2mdu	765	9	-	-	HYPH
cord-014597-66vd2mdu	765	10	fed	fed	JJ
cord-014597-66vd2mdu	765	11	technology	technology	NN
cord-014597-66vd2mdu	765	12	with	with	IN
cord-014597-66vd2mdu	765	13	continuous	continuous	JJ
cord-014597-66vd2mdu	765	14	multi	multi	JJ
cord-014597-66vd2mdu	765	15	-	-	JJ
cord-014597-66vd2mdu	765	16	column	column	JJ
cord-014597-66vd2mdu	765	17	capture	capture	NN
cord-014597-66vd2mdu	765	18	.	.	.
cord-014597-66vd2mdu	766	1	An	an	DT
cord-014597-66vd2mdu	766	2	advanced	advanced	JJ
cord-014597-66vd2mdu	766	3	cell	cell	NN
cord-014597-66vd2mdu	766	4	culture	culture	NN
cord-014597-66vd2mdu	766	5	clarification	clarification	NN
cord-014597-66vd2mdu	766	6	method	method	NN
cord-014597-66vd2mdu	766	7	was	be	VBD
cord-014597-66vd2mdu	766	8	introduced	introduce	VBN
cord-014597-66vd2mdu	766	9	to	to	TO
cord-014597-66vd2mdu	766	10	simplify	simplify	VB
cord-014597-66vd2mdu	766	11	downstream	downstream	JJ
cord-014597-66vd2mdu	766	12	operations	operation	NNS
cord-014597-66vd2mdu	766	13	and	and	CC
cord-014597-66vd2mdu	766	14	increase	increase	VB
cord-014597-66vd2mdu	766	15	overall	overall	JJ
cord-014597-66vd2mdu	766	16	cost	cost	NN
cord-014597-66vd2mdu	766	17	-	-	HYPH
cord-014597-66vd2mdu	766	18	effectiveness	effectiveness	NN
cord-014597-66vd2mdu	766	19	of	of	IN
cord-014597-66vd2mdu	766	20	the	the	DT
cord-014597-66vd2mdu	766	21	process	process	NN
cord-014597-66vd2mdu	766	22	,	,	,
cord-014597-66vd2mdu	766	23	for	for	IN
cord-014597-66vd2mdu	766	24	an	an	DT
cord-014597-66vd2mdu	766	25	optimized	optimize	VBN
cord-014597-66vd2mdu	766	26	production	production	NN
cord-014597-66vd2mdu	766	27	of	of	IN
cord-014597-66vd2mdu	766	28	recombinant	recombinant	JJ
cord-014597-66vd2mdu	766	29	proteins	protein	NNS
cord-014597-66vd2mdu	766	30	.	.	.
cord-014597-66vd2mdu	767	1	This	this	DT
cord-014597-66vd2mdu	767	2	study	study	NN
cord-014597-66vd2mdu	767	3	was	be	VBD
cord-014597-66vd2mdu	767	4	performed	perform	VBN
cord-014597-66vd2mdu	767	5	with	with	IN
cord-014597-66vd2mdu	767	6	CHO	CHO	NNP
cord-014597-66vd2mdu	767	7	cells	cell	NNS
cord-014597-66vd2mdu	767	8	expressing	express	VBG
cord-014597-66vd2mdu	767	9	a	a	DT
cord-014597-66vd2mdu	767	10	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	767	11	antibody	antibody	NN
cord-014597-66vd2mdu	767	12	targeted	target	VBN
cord-014597-66vd2mdu	767	13	against	against	IN
cord-014597-66vd2mdu	767	14	the	the	DT
cord-014597-66vd2mdu	767	15	Coronoavirus	Coronoavirus	NNP
cord-014597-66vd2mdu	767	16	responsible	responsible	JJ
cord-014597-66vd2mdu	767	17	for	for	IN
cord-014597-66vd2mdu	767	18	the	the	DT
cord-014597-66vd2mdu	767	19	Middle	Middle	NNP
cord-014597-66vd2mdu	767	20	East	East	NNP
cord-014597-66vd2mdu	767	21	Respiratory	Respiratory	NNP
cord-014597-66vd2mdu	767	22	Syndrome	Syndrome	NNP
cord-014597-66vd2mdu	767	23	(	(	-LRB-
cord-014597-66vd2mdu	767	24	MERS	MERS	NNP
cord-014597-66vd2mdu	767	25	)	)	-RRB-
cord-014597-66vd2mdu	767	26	,	,	,
cord-014597-66vd2mdu	767	27	developed	develop	VBN
cord-014597-66vd2mdu	767	28	by	by	IN
cord-014597-66vd2mdu	767	29	Organic	Organic	NNP
cord-014597-66vd2mdu	767	30	Vaccines	Vaccines	NNPS
cord-014597-66vd2mdu	767	31	TM	TM	NNP
cord-014597-66vd2mdu	767	32	and	and	CC
cord-014597-66vd2mdu	767	33	the	the	DT
cord-014597-66vd2mdu	767	34	NIH	NIH	NNP
cord-014597-66vd2mdu	767	35	,	,	,
cord-014597-66vd2mdu	767	36	kindly	kindly	RB
cord-014597-66vd2mdu	767	37	provided	provide	VBD
cord-014597-66vd2mdu	767	38	to	to	IN
cord-014597-66vd2mdu	767	39	Univercells	Univercells	NNP
cord-014597-66vd2mdu	767	40	.	.	.
cord-014597-66vd2mdu	768	1	Upstream	upstream	JJ
cord-014597-66vd2mdu	768	2	process	process	NN
cord-014597-66vd2mdu	768	3	:	:	:
cord-014597-66vd2mdu	768	4	-Fed	-Fed	NFP
cord-014597-66vd2mdu	768	5	-	-	HYPH
cord-014597-66vd2mdu	768	6	batch	batch	NN
cord-014597-66vd2mdu	768	7	,	,	,
cord-014597-66vd2mdu	768	8	12	12	CD
cord-014597-66vd2mdu	768	9	days	day	NNS
cord-014597-66vd2mdu	768	10	culture	culture	NN
cord-014597-66vd2mdu	768	11	at	at	IN
cord-014597-66vd2mdu	768	12	10L	10l	JJ
cord-014597-66vd2mdu	768	13	scale	scale	NN
cord-014597-66vd2mdu	768	14	with	with	IN
cord-014597-66vd2mdu	768	15	CD	CD	NNP
cord-014597-66vd2mdu	768	16	-	-	HYPH
cord-014597-66vd2mdu	768	17	CHO	CHO	NNP
cord-014597-66vd2mdu	768	18	chemically	chemically	RB
cord-014597-66vd2mdu	768	19	defined	define	VBD
cord-014597-66vd2mdu	768	20	media	medium	NNS
cord-014597-66vd2mdu	768	21	and	and	CC
cord-014597-66vd2mdu	768	22	feeds	feed	NNS
cord-014597-66vd2mdu	768	23	.	.	.
cord-014597-66vd2mdu	769	1	Harvest	harvest	NN
cord-014597-66vd2mdu	769	2	treatment	treatment	NN
cord-014597-66vd2mdu	769	3	:	:	:
cord-014597-66vd2mdu	770	1	-Precipitation	-precipitation	NN
cord-014597-66vd2mdu	770	2	of	of	IN
cord-014597-66vd2mdu	770	3	impurities	impurity	NNS
cord-014597-66vd2mdu	770	4	in	in	IN
cord-014597-66vd2mdu	770	5	the	the	DT
cord-014597-66vd2mdu	770	6	production	production	NN
cord-014597-66vd2mdu	770	7	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	770	8	using	use	VBG
cord-014597-66vd2mdu	770	9	organic	organic	JJ
cord-014597-66vd2mdu	770	10	compounds	compound	NNS
cord-014597-66vd2mdu	770	11	(	(	-LRB-
cord-014597-66vd2mdu	770	12	<	<	NNP
cord-014597-66vd2mdu	770	13	1	1	CD
cord-014597-66vd2mdu	770	14	%	%	NN
cord-014597-66vd2mdu	770	15	v	v	NN
cord-014597-66vd2mdu	770	16	/	/	SYM
cord-014597-66vd2mdu	770	17	v	v	NN
cord-014597-66vd2mdu	770	18	)	)	-RRB-
cord-014597-66vd2mdu	770	19	and	and	CC
cord-014597-66vd2mdu	770	20	flocculation	flocculation	NN
cord-014597-66vd2mdu	770	21	by	by	IN
cord-014597-66vd2mdu	770	22	electropositive	electropositive	JJ
cord-014597-66vd2mdu	770	23	organics	organic	NNS
cord-014597-66vd2mdu	770	24	(	(	-LRB-
cord-014597-66vd2mdu	770	25	<	<	XX
cord-014597-66vd2mdu	770	26	0.1	0.1	CD
cord-014597-66vd2mdu	770	27	%	%	NN
cord-014597-66vd2mdu	770	28	w	w	NN
cord-014597-66vd2mdu	770	29	/	/	SYM
cord-014597-66vd2mdu	770	30	v	v	NN
cord-014597-66vd2mdu	770	31	)	)	-RRB-
cord-014597-66vd2mdu	770	32	.	.	.
cord-014597-66vd2mdu	771	1	-Acidic	-Acidic	NNP
cord-014597-66vd2mdu	772	1	pH	pH	NNP
cord-014597-66vd2mdu	772	2	and	and	CC
cord-014597-66vd2mdu	772	3	physiological	physiological	JJ
cord-014597-66vd2mdu	772	4	conductivity	conductivity	NN
cord-014597-66vd2mdu	772	5	.	.	.
cord-014597-66vd2mdu	772	6	 	 	_SP
cord-014597-66vd2mdu	773	1	Upstream	upstream	JJ
cord-014597-66vd2mdu	773	2	processing	processing	NN
cord-014597-66vd2mdu	773	3	and	and	CC
cord-014597-66vd2mdu	773	4	harvest	harvest	NN
cord-014597-66vd2mdu	773	5	treatment	treatment	NN
cord-014597-66vd2mdu	773	6	:	:	:
cord-014597-66vd2mdu	773	7	Culture	culture	NN
cord-014597-66vd2mdu	773	8	reached	reach	VBD
cord-014597-66vd2mdu	773	9	0.5	0.5	CD
cord-014597-66vd2mdu	773	10	g/	g/	NN
cord-014597-66vd2mdu	773	11	L	l	NN
cord-014597-66vd2mdu	773	12	(	(	-LRB-
cord-014597-66vd2mdu	773	13	8x10	8x10	CD
cord-014597-66vd2mdu	773	14	6	6	CD
cord-014597-66vd2mdu	773	15	cells	cell	NNS
cord-014597-66vd2mdu	773	16	/	/	SYM
cord-014597-66vd2mdu	773	17	mL	ml	NN
cord-014597-66vd2mdu	773	18	;	;	:
cord-014597-66vd2mdu	773	19	90	90	CD
cord-014597-66vd2mdu	773	20	%	%	NN
cord-014597-66vd2mdu	773	21	viability	viability	NN
cord-014597-66vd2mdu	773	22	)	)	-RRB-
cord-014597-66vd2mdu	773	23	,	,	,
cord-014597-66vd2mdu	773	24	harvest	harvest	NN
cord-014597-66vd2mdu	773	25	treatment	treatment	NN
cord-014597-66vd2mdu	773	26	was	be	VBD
cord-014597-66vd2mdu	773	27	found	find	VBN
cord-014597-66vd2mdu	773	28	to	to	TO
cord-014597-66vd2mdu	773	29	be	be	VB
cord-014597-66vd2mdu	773	30	very	very	RB
cord-014597-66vd2mdu	773	31	effective	effective	JJ
cord-014597-66vd2mdu	773	32	in	in	IN
cord-014597-66vd2mdu	773	33	terms	term	NNS
cord-014597-66vd2mdu	773	34	of	of	IN
cord-014597-66vd2mdu	773	35	impurities	impurity	NNS
cord-014597-66vd2mdu	773	36	clearance	clearance	NN
cord-014597-66vd2mdu	773	37	.	.	.
cord-014597-66vd2mdu	774	1	Capture	capture	VB
cord-014597-66vd2mdu	774	2	:	:	:
cord-014597-66vd2mdu	775	1	Capture	capture	NN
cord-014597-66vd2mdu	775	2	strategies	strategy	NNS
cord-014597-66vd2mdu	775	3	were	be	VBD
cord-014597-66vd2mdu	775	4	evaluated	evaluate	VBN
cord-014597-66vd2mdu	775	5	from	from	IN
cord-014597-66vd2mdu	775	6	the	the	DT
cord-014597-66vd2mdu	775	7	point	point	NN
cord-014597-66vd2mdu	775	8	of	of	IN
cord-014597-66vd2mdu	775	9	view	view	NN
cord-014597-66vd2mdu	775	10	of	of	IN
cord-014597-66vd2mdu	775	11	simplification	simplification	NN
cord-014597-66vd2mdu	775	12	of	of	IN
cord-014597-66vd2mdu	775	13	downstream	downstream	JJ
cord-014597-66vd2mdu	775	14	operations	operation	NNS
cord-014597-66vd2mdu	775	15	,	,	,
cord-014597-66vd2mdu	775	16	with	with	IN
cord-014597-66vd2mdu	775	17	HCP	HCP	NNP
cord-014597-66vd2mdu	775	18	impurities	impurity	NNS
cord-014597-66vd2mdu	775	19	content	content	NN
cord-014597-66vd2mdu	775	20	monitored	monitor	VBN
cord-014597-66vd2mdu	775	21	as	as	IN
cord-014597-66vd2mdu	775	22	a	a	DT
cord-014597-66vd2mdu	775	23	key	key	JJ
cord-014597-66vd2mdu	775	24	performance	performance	NN
cord-014597-66vd2mdu	775	25	indicator	indicator	NN
cord-014597-66vd2mdu	775	26	.	.	.
cord-014597-66vd2mdu	776	1	-Protein	-protein	RB
cord-014597-66vd2mdu	777	1	A	a	DT
cord-014597-66vd2mdu	777	2	affinity	affinity	NN
cord-014597-66vd2mdu	777	3	chromatography	chromatography	NN
cord-014597-66vd2mdu	777	4	:	:	:
cord-014597-66vd2mdu	778	1	Advanced	advanced	JJ
cord-014597-66vd2mdu	778	2	harvest	harvest	NN
cord-014597-66vd2mdu	778	3	clarification	clarification	NN
cord-014597-66vd2mdu	778	4	enabled	enable	VBD
cord-014597-66vd2mdu	778	5	major	major	JJ
cord-014597-66vd2mdu	778	6	improvements	improvement	NNS
cord-014597-66vd2mdu	778	7	in	in	IN
cord-014597-66vd2mdu	778	8	affinity	affinity	NN
cord-014597-66vd2mdu	778	9	capture	capture	NN
cord-014597-66vd2mdu	778	10	,	,	,
cord-014597-66vd2mdu	778	11	in	in	IN
cord-014597-66vd2mdu	778	12	terms	term	NNS
cord-014597-66vd2mdu	778	13	of	of	IN
cord-014597-66vd2mdu	778	14	eluate	eluate	JJ
cord-014597-66vd2mdu	778	15	purity	purity	NN
cord-014597-66vd2mdu	778	16	and	and	CC
cord-014597-66vd2mdu	778	17	reduction	reduction	NN
cord-014597-66vd2mdu	778	18	of	of	IN
cord-014597-66vd2mdu	778	19	host	host	NN
cord-014597-66vd2mdu	778	20	cell	cell	NN
cord-014597-66vd2mdu	778	21	impurities	impurity	NNS
cord-014597-66vd2mdu	778	22	(	(	-LRB-
cord-014597-66vd2mdu	778	23	<	<	NNP
cord-014597-66vd2mdu	778	24	35	35	CD
cord-014597-66vd2mdu	778	25	ppm	ppm	NN
cord-014597-66vd2mdu	778	26	in	in	IN
cord-014597-66vd2mdu	778	27	all	all	DT
cord-014597-66vd2mdu	778	28	conditions	condition	NNS
cord-014597-66vd2mdu	778	29	tested	test	VBN
cord-014597-66vd2mdu	778	30	)	)	-RRB-
cord-014597-66vd2mdu	778	31	.	.	.
cord-014597-66vd2mdu	779	1	(	(	-LRB-
cord-014597-66vd2mdu	779	2	Fig	Fig	NNP
cord-014597-66vd2mdu	779	3	.	.	NNP
cord-014597-66vd2mdu	779	4	1	1	CD
cord-014597-66vd2mdu	779	5	)	)	-RRB-
cord-014597-66vd2mdu	779	6	.	.	.
cord-014597-66vd2mdu	780	1	-Cation	-cation	NN
cord-014597-66vd2mdu	780	2	exchange	exchange	NN
cord-014597-66vd2mdu	780	3	chromatography	chromatography	NN
cord-014597-66vd2mdu	780	4	:	:	:
cord-014597-66vd2mdu	780	5	CEx	CEx	NNS
cord-014597-66vd2mdu	780	6	allows	allow	VBZ
cord-014597-66vd2mdu	780	7	higher	high	JJR
cord-014597-66vd2mdu	780	8	capacities	capacity	NNS
cord-014597-66vd2mdu	780	9	(	(	-LRB-
cord-014597-66vd2mdu	780	10	>	>	VBZ
cord-014597-66vd2mdu	780	11	100	100	CD
cord-014597-66vd2mdu	780	12	g	g	NN
cord-014597-66vd2mdu	780	13	/	/	SYM
cord-014597-66vd2mdu	780	14	L	L	NNP
cord-014597-66vd2mdu	780	15	)	)	-RRB-
cord-014597-66vd2mdu	780	16	than	than	IN
cord-014597-66vd2mdu	780	17	protein	protein	NN
cord-014597-66vd2mdu	780	18	A	A	NNP
cord-014597-66vd2mdu	780	19	,	,	,
cord-014597-66vd2mdu	780	20	whilst	whilst	IN
cord-014597-66vd2mdu	780	21	being	be	VBG
cord-014597-66vd2mdu	780	22	more	more	RBR
cord-014597-66vd2mdu	780	23	affordable	affordable	JJ
cord-014597-66vd2mdu	780	24	(	(	-LRB-
cord-014597-66vd2mdu	780	25	from	from	IN
cord-014597-66vd2mdu	780	26	2-to	2-to	CD
cord-014597-66vd2mdu	780	27	6-fold	6-fold	CD
cord-014597-66vd2mdu	780	28	cheaper	cheap	JJR
cord-014597-66vd2mdu	780	29	)	)	-RRB-
cord-014597-66vd2mdu	780	30	.	.	.
cord-014597-66vd2mdu	781	1	Low	low	JJ
cord-014597-66vd2mdu	781	2	residual	residual	JJ
cord-014597-66vd2mdu	781	3	HCP	HCP	NNP
cord-014597-66vd2mdu	781	4	(	(	-LRB-
cord-014597-66vd2mdu	781	5	<	<	XX
cord-014597-66vd2mdu	781	6	500	500	CD
cord-014597-66vd2mdu	781	7	ppm	ppm	NN
cord-014597-66vd2mdu	781	8	)	)	-RRB-
cord-014597-66vd2mdu	781	9	was	be	VBD
cord-014597-66vd2mdu	781	10	observed	observe	VBN
cord-014597-66vd2mdu	781	11	with	with	IN
cord-014597-66vd2mdu	781	12	all	all	DT
cord-014597-66vd2mdu	781	13	CEx	CEx	NNS
cord-014597-66vd2mdu	781	14	resins	resin	NNS
cord-014597-66vd2mdu	781	15	tested	test	VBN
cord-014597-66vd2mdu	781	16	.	.	.
cord-014597-66vd2mdu	782	1	Without	without	IN
cord-014597-66vd2mdu	782	2	harvest	harvest	NN
cord-014597-66vd2mdu	782	3	treatment	treatment	NN
cord-014597-66vd2mdu	782	4	and	and	CC
cord-014597-66vd2mdu	782	5	clarification	clarification	NN
cord-014597-66vd2mdu	782	6	preceding	precede	VBG
cord-014597-66vd2mdu	782	7	the	the	DT
cord-014597-66vd2mdu	782	8	capture	capture	NN
cord-014597-66vd2mdu	782	9	studies	study	NNS
cord-014597-66vd2mdu	782	10	(	(	-LRB-
cord-014597-66vd2mdu	782	11	either	either	CC
cord-014597-66vd2mdu	782	12	affinity	affinity	NN
cord-014597-66vd2mdu	782	13	or	or	CC
cord-014597-66vd2mdu	782	14	CEx	CEx	NNS
cord-014597-66vd2mdu	782	15	)	)	-RRB-
cord-014597-66vd2mdu	782	16	,	,	,
cord-014597-66vd2mdu	782	17	results	result	NNS
cord-014597-66vd2mdu	782	18	showed	show	VBD
cord-014597-66vd2mdu	782	19	a	a	DT
cord-014597-66vd2mdu	782	20	lower	low	JJR
cord-014597-66vd2mdu	782	21	binding	binding	NN
cord-014597-66vd2mdu	782	22	capacity	capacity	NN
cord-014597-66vd2mdu	782	23	of	of	IN
cord-014597-66vd2mdu	782	24	the	the	DT
cord-014597-66vd2mdu	782	25	resin	resin	NN
cord-014597-66vd2mdu	782	26	,	,	,
cord-014597-66vd2mdu	782	27	a	a	DT
cord-014597-66vd2mdu	782	28	higher	high	JJR
cord-014597-66vd2mdu	782	29	content	content	NN
cord-014597-66vd2mdu	782	30	of	of	IN
cord-014597-66vd2mdu	782	31	HCP	HCP	NNP
cord-014597-66vd2mdu	782	32	in	in	IN
cord-014597-66vd2mdu	782	33	the	the	DT
cord-014597-66vd2mdu	782	34	eluate	eluate	NN
cord-014597-66vd2mdu	782	35	(	(	-LRB-
cord-014597-66vd2mdu	782	36	up	up	IN
cord-014597-66vd2mdu	782	37	to	to	TO
cord-014597-66vd2mdu	782	38	2000	2000	CD
cord-014597-66vd2mdu	782	39	ppm	ppm	NN
cord-014597-66vd2mdu	782	40	)	)	-RRB-
cord-014597-66vd2mdu	782	41	,	,	,
cord-014597-66vd2mdu	782	42	a	a	DT
cord-014597-66vd2mdu	782	43	higher	high	JJR
cord-014597-66vd2mdu	782	44	content	content	NN
cord-014597-66vd2mdu	782	45	of	of	IN
cord-014597-66vd2mdu	782	46	HMW	HMW	NNP
cord-014597-66vd2mdu	782	47	species	specie	NNS
cord-014597-66vd2mdu	782	48	in	in	IN
cord-014597-66vd2mdu	782	49	the	the	DT
cord-014597-66vd2mdu	782	50	elution	elution	NN
cord-014597-66vd2mdu	782	51	fraction	fraction	NN
cord-014597-66vd2mdu	782	52	(	(	-LRB-
cord-014597-66vd2mdu	782	53	up	up	IN
cord-014597-66vd2mdu	782	54	to	to	TO
cord-014597-66vd2mdu	782	55	3-fold	3-fold	CD
cord-014597-66vd2mdu	782	56	higher	high	JJR
cord-014597-66vd2mdu	782	57	)	)	-RRB-
cord-014597-66vd2mdu	782	58	and	and	CC
cord-014597-66vd2mdu	782	59	a	a	DT
cord-014597-66vd2mdu	782	60	significant	significant	JJ
cord-014597-66vd2mdu	782	61	turbidity	turbidity	NN
cord-014597-66vd2mdu	782	62	of	of	IN
cord-014597-66vd2mdu	782	63	the	the	DT
cord-014597-66vd2mdu	782	64	neutralized	neutralize	VBN
cord-014597-66vd2mdu	782	65	eluate	eluate	NN
cord-014597-66vd2mdu	782	66	.	.	.
cord-014597-66vd2mdu	783	1	-Continuous	-continuous	JJ
cord-014597-66vd2mdu	783	2	multicolumn	multicolumn	NN
cord-014597-66vd2mdu	783	3	chromatography	chromatography	NN
cord-014597-66vd2mdu	783	4	:	:	:
cord-014597-66vd2mdu	783	5	Further	further	JJ
cord-014597-66vd2mdu	783	6	options	option	NNS
cord-014597-66vd2mdu	783	7	to	to	TO
cord-014597-66vd2mdu	783	8	increase	increase	VB
cord-014597-66vd2mdu	783	9	cost	cost	NN
cord-014597-66vd2mdu	783	10	efficiency	efficiency	NN
cord-014597-66vd2mdu	783	11	include	include	VBP
cord-014597-66vd2mdu	783	12	using	use	VBG
cord-014597-66vd2mdu	783	13	a	a	DT
cord-014597-66vd2mdu	783	14	continuous	continuous	JJ
cord-014597-66vd2mdu	783	15	multicolumn	multicolumn	NN
cord-014597-66vd2mdu	783	16	setup	setup	NN
cord-014597-66vd2mdu	783	17	(	(	-LRB-
cord-014597-66vd2mdu	783	18	Table	table	NN
cord-014597-66vd2mdu	783	19	1	1	CD
cord-014597-66vd2mdu	783	20	)	)	-RRB-
cord-014597-66vd2mdu	783	21	.	.	.
cord-014597-66vd2mdu	784	1	Two	two	CD
cord-014597-66vd2mdu	784	2	models	model	NNS
cord-014597-66vd2mdu	784	3	were	be	VBD
cord-014597-66vd2mdu	784	4	assessed	assess	VBN
cord-014597-66vd2mdu	784	5	based	base	VBN
cord-014597-66vd2mdu	784	6	on	on	IN
cord-014597-66vd2mdu	784	7	two	two	CD
cord-014597-66vd2mdu	784	8	different	different	JJ
cord-014597-66vd2mdu	784	9	static	static	JJ
cord-014597-66vd2mdu	784	10	binding	binding	NN
cord-014597-66vd2mdu	784	11	capacities	capacity	NNS
cord-014597-66vd2mdu	784	12	(	(	-LRB-
cord-014597-66vd2mdu	784	13	SBC	SBC	NNP
cord-014597-66vd2mdu	784	14	)	)	-RRB-
cord-014597-66vd2mdu	784	15	,	,	,
cord-014597-66vd2mdu	784	16	demonstrating	demonstrate	VBG
cord-014597-66vd2mdu	784	17	that	that	IN
cord-014597-66vd2mdu	784	18	4	4	CD
cord-014597-66vd2mdu	784	19	to	to	TO
cord-014597-66vd2mdu	784	20	6	6	CD
cord-014597-66vd2mdu	784	21	columns	column	NNS
cord-014597-66vd2mdu	784	22	of	of	IN
cord-014597-66vd2mdu	784	23	100ml	100ml	NNP
cord-014597-66vd2mdu	784	24	were	be	VBD
cord-014597-66vd2mdu	784	25	able	able	JJ
cord-014597-66vd2mdu	784	26	to	to	TO
cord-014597-66vd2mdu	784	27	process	process	VB
cord-014597-66vd2mdu	784	28	a	a	DT
cord-014597-66vd2mdu	784	29	200L	200l	CD
cord-014597-66vd2mdu	784	30	production	production	NN
cord-014597-66vd2mdu	784	31	in	in	IN
cord-014597-66vd2mdu	784	32	less	less	JJR
cord-014597-66vd2mdu	784	33	than	than	IN
cord-014597-66vd2mdu	784	34	24h	24h	NNS
cord-014597-66vd2mdu	784	35	.	.	.
cord-014597-66vd2mdu	785	1	This	this	DT
cord-014597-66vd2mdu	785	2	method	method	NN
cord-014597-66vd2mdu	785	3	provides	provide	VBZ
cord-014597-66vd2mdu	785	4	a	a	DT
cord-014597-66vd2mdu	785	5	great	great	JJ
cord-014597-66vd2mdu	785	6	opportunity	opportunity	NN
cord-014597-66vd2mdu	785	7	for	for	IN
cord-014597-66vd2mdu	785	8	designing	design	VBG
cord-014597-66vd2mdu	785	9	simplified	simplified	JJ
cord-014597-66vd2mdu	785	10	and	and	CC
cord-014597-66vd2mdu	785	11	low	low	JJ
cord-014597-66vd2mdu	785	12	footprint	footprint	NN
cord-014597-66vd2mdu	785	13	Mabs	Mabs	NNP
cord-014597-66vd2mdu	785	14	DSP	DSP	NNP
cord-014597-66vd2mdu	785	15	processes	process	NNS
cord-014597-66vd2mdu	785	16	,	,	,
cord-014597-66vd2mdu	785	17	while	while	IN
cord-014597-66vd2mdu	785	18	maintaining	maintain	VBG
cord-014597-66vd2mdu	785	19	similar	similar	JJ
cord-014597-66vd2mdu	785	20	or	or	CC
cord-014597-66vd2mdu	785	21	achieving	achieve	VBG
cord-014597-66vd2mdu	785	22	superior	superior	JJ
cord-014597-66vd2mdu	785	23	quality	quality	NN
cord-014597-66vd2mdu	785	24	profile	profile	NN
cord-014597-66vd2mdu	785	25	compared	compare	VBN
cord-014597-66vd2mdu	785	26	to	to	IN
cord-014597-66vd2mdu	785	27	standard	standard	JJ
cord-014597-66vd2mdu	785	28	approaches	approach	NNS
cord-014597-66vd2mdu	785	29	:	:	:
cord-014597-66vd2mdu	786	1	-Harvests	-harvest	NNS
cord-014597-66vd2mdu	786	2	treatments	treatment	NNS
cord-014597-66vd2mdu	786	3	followed	follow	VBN
cord-014597-66vd2mdu	786	4	by	by	IN
cord-014597-66vd2mdu	786	5	depth	depth	NN
cord-014597-66vd2mdu	786	6	filtration	filtration	NN
cord-014597-66vd2mdu	786	7	proved	prove	VBN
cord-014597-66vd2mdu	786	8	to	to	TO
cord-014597-66vd2mdu	786	9	be	be	VB
cord-014597-66vd2mdu	786	10	a	a	DT
cord-014597-66vd2mdu	786	11	cost	cost	NN
cord-014597-66vd2mdu	786	12	-	-	HYPH
cord-014597-66vd2mdu	786	13	efficient	efficient	JJ
cord-014597-66vd2mdu	786	14	way	way	NN
cord-014597-66vd2mdu	786	15	to	to	TO
cord-014597-66vd2mdu	786	16	obtain	obtain	VB
cord-014597-66vd2mdu	786	17	pretreated	pretreate	VBN
cord-014597-66vd2mdu	786	18	feed	feed	NN
cord-014597-66vd2mdu	786	19	and	and	CC
cord-014597-66vd2mdu	786	20	minimize	minimize	VB
cord-014597-66vd2mdu	786	21	the	the	DT
cord-014597-66vd2mdu	786	22	burden	burden	NN
cord-014597-66vd2mdu	786	23	on	on	IN
cord-014597-66vd2mdu	786	24	downstream	downstream	JJ
cord-014597-66vd2mdu	786	25	operations	operation	NNS
cord-014597-66vd2mdu	786	26	.	.	.
cord-014597-66vd2mdu	787	1	-Protein	-protein	RB
cord-014597-66vd2mdu	788	1	A	a	DT
cord-014597-66vd2mdu	788	2	resins	resin	NNS
cord-014597-66vd2mdu	788	3	exhibited	exhibit	VBD
cord-014597-66vd2mdu	788	4	advantages	advantage	NNS
cord-014597-66vd2mdu	788	5	of	of	IN
cord-014597-66vd2mdu	788	6	extracting	extract	VBG
cord-014597-66vd2mdu	788	7	key	key	JJ
cord-014597-66vd2mdu	788	8	contaminants	contaminant	NNS
cord-014597-66vd2mdu	788	9	during	during	IN
cord-014597-66vd2mdu	788	10	harvest	harvest	NN
cord-014597-66vd2mdu	788	11	treatment	treatment	NN
cord-014597-66vd2mdu	788	12	,	,	,
cord-014597-66vd2mdu	788	13	while	while	IN
cord-014597-66vd2mdu	788	14	CAEX	CAEX	NNP
cord-014597-66vd2mdu	788	15	confirmed	confirm	VBD
cord-014597-66vd2mdu	788	16	to	to	TO
cord-014597-66vd2mdu	788	17	be	be	VB
cord-014597-66vd2mdu	788	18	a	a	DT
cord-014597-66vd2mdu	788	19	competitive	competitive	JJ
cord-014597-66vd2mdu	788	20	capture	capture	NN
cord-014597-66vd2mdu	788	21	strategy	strategy	NN
cord-014597-66vd2mdu	788	22	.	.	.
cord-014597-66vd2mdu	789	1	-Switching	-switche	VBG
cord-014597-66vd2mdu	789	2	from	from	IN
cord-014597-66vd2mdu	789	3	batch	batch	NN
cord-014597-66vd2mdu	789	4	to	to	IN
cord-014597-66vd2mdu	789	5	continuous	continuous	JJ
cord-014597-66vd2mdu	789	6	multicolumn	multicolumn	NN
cord-014597-66vd2mdu	789	7	mode	mode	NN
cord-014597-66vd2mdu	789	8	allowed	allow	VBN
cord-014597-66vd2mdu	789	9	to	to	TO
cord-014597-66vd2mdu	789	10	process	process	VB
cord-014597-66vd2mdu	789	11	a	a	DT
cord-014597-66vd2mdu	789	12	complete	complete	JJ
cord-014597-66vd2mdu	789	13	batch	batch	NN
cord-014597-66vd2mdu	789	14	in	in	IN
cord-014597-66vd2mdu	789	15	less	less	JJR
cord-014597-66vd2mdu	789	16	than	than	IN
cord-014597-66vd2mdu	789	17	24	24	CD
cord-014597-66vd2mdu	789	18	hours	hour	NNS
cord-014597-66vd2mdu	789	19	,	,	,
cord-014597-66vd2mdu	789	20	requiring	require	VBG
cord-014597-66vd2mdu	789	21	lower	low	JJR
cord-014597-66vd2mdu	789	22	media	medium	NNS
cord-014597-66vd2mdu	789	23	and	and	CC
cord-014597-66vd2mdu	789	24	resins	resin	NNS
cord-014597-66vd2mdu	789	25	volumes	volume	NNS
cord-014597-66vd2mdu	789	26	.	.	.
cord-014597-66vd2mdu	790	1	Followed	follow	VBN
cord-014597-66vd2mdu	790	2	by	by	IN
cord-014597-66vd2mdu	790	3	a	a	DT
cord-014597-66vd2mdu	790	4	single	single	JJ
cord-014597-66vd2mdu	790	5	polishing	polishing	NN
cord-014597-66vd2mdu	790	6	step	step	NN
cord-014597-66vd2mdu	790	7	,	,	,
cord-014597-66vd2mdu	790	8	such	such	JJ
cord-014597-66vd2mdu	790	9	process	process	NN
cord-014597-66vd2mdu	790	10	set	set	VBN
cord-014597-66vd2mdu	790	11	-	-	HYPH
cord-014597-66vd2mdu	790	12	up	up	NN
cord-014597-66vd2mdu	790	13	strongly	strongly	RB
cord-014597-66vd2mdu	790	14	supports	support	VBZ
cord-014597-66vd2mdu	790	15	the	the	DT
cord-014597-66vd2mdu	790	16	reduction	reduction	NN
cord-014597-66vd2mdu	790	17	of	of	IN
cord-014597-66vd2mdu	790	18	operations	operation	NNS
cord-014597-66vd2mdu	790	19	required	require	VBN
cord-014597-66vd2mdu	790	20	to	to	TO
cord-014597-66vd2mdu	790	21	deliver	deliver	VB
cord-014597-66vd2mdu	790	22	a	a	DT
cord-014597-66vd2mdu	790	23	high	high	JJ
cord-014597-66vd2mdu	790	24	-	-	HYPH
cord-014597-66vd2mdu	790	25	quality	quality	NN
cord-014597-66vd2mdu	790	26	product	product	NN
cord-014597-66vd2mdu	790	27	.	.	.
cord-014597-66vd2mdu	791	1	Analyses	analysis	NNS
cord-014597-66vd2mdu	791	2	of	of	IN
cord-014597-66vd2mdu	791	3	product	product	NN
cord-014597-66vd2mdu	791	4	quality	quality	NN
cord-014597-66vd2mdu	791	5	of	of	IN
cord-014597-66vd2mdu	791	6	complex	complex	JJ
cord-014597-66vd2mdu	791	7	polymeric	polymeric	JJ
cord-014597-66vd2mdu	791	8	IgM	igm	NN
cord-014597-66vd2mdu	791	9	produced	produce	VBN
cord-014597-66vd2mdu	791	10	by	by	IN
cord-014597-66vd2mdu	791	11	CHO	CHO	NNP
cord-014597-66vd2mdu	791	12	cells	cell	NNS
cord-014597-66vd2mdu	792	1	Background	Background	NNP
cord-014597-66vd2mdu	792	2	Immunoglobulin	Immunoglobulin	NNP
cord-014597-66vd2mdu	792	3	M	M	NNP
cord-014597-66vd2mdu	792	4	(	(	-LRB-
cord-014597-66vd2mdu	792	5	IgM	IgM	NNP
cord-014597-66vd2mdu	792	6	)	)	-RRB-
cord-014597-66vd2mdu	792	7	antibodies	antibody	NNS
cord-014597-66vd2mdu	792	8	are	be	VBP
cord-014597-66vd2mdu	792	9	secreted	secrete	VBN
cord-014597-66vd2mdu	792	10	by	by	IN
cord-014597-66vd2mdu	792	11	B	b	NN
cord-014597-66vd2mdu	792	12	cells	cell	NNS
cord-014597-66vd2mdu	792	13	as	as	IN
cord-014597-66vd2mdu	792	14	the	the	DT
cord-014597-66vd2mdu	792	15	first	first	JJ
cord-014597-66vd2mdu	792	16	defense	defense	NN
cord-014597-66vd2mdu	792	17	against	against	IN
cord-014597-66vd2mdu	792	18	invading	invade	VBG
cord-014597-66vd2mdu	792	19	pathogens	pathogen	NNS
cord-014597-66vd2mdu	792	20	during	during	IN
cord-014597-66vd2mdu	792	21	primary	primary	JJ
cord-014597-66vd2mdu	792	22	immune	immune	JJ
cord-014597-66vd2mdu	792	23	response	response	NN
cord-014597-66vd2mdu	792	24	.	.	.
cord-014597-66vd2mdu	793	1	Some	some	DT
cord-014597-66vd2mdu	793	2	IgM	IgM	NNP
cord-014597-66vd2mdu	793	3	antibodies	antibody	NNS
cord-014597-66vd2mdu	793	4	already	already	RB
cord-014597-66vd2mdu	793	5	gained	gain	VBD
cord-014597-66vd2mdu	793	6	the	the	DT
cord-014597-66vd2mdu	793	7	orphan	orphan	NN
cord-014597-66vd2mdu	793	8	drug	drug	NN
cord-014597-66vd2mdu	793	9	status	status	NN
cord-014597-66vd2mdu	793	10	,	,	,
cord-014597-66vd2mdu	793	11	which	which	WDT
cord-014597-66vd2mdu	793	12	shows	show	VBZ
cord-014597-66vd2mdu	793	13	their	-PRON-	PRP$
cord-014597-66vd2mdu	793	14	unique	unique	JJ
cord-014597-66vd2mdu	793	15	capability	capability	NN
cord-014597-66vd2mdu	793	16	in	in	IN
cord-014597-66vd2mdu	793	17	therapy	therapy	NN
cord-014597-66vd2mdu	793	18	of	of	IN
cord-014597-66vd2mdu	793	19	rare	rare	JJ
cord-014597-66vd2mdu	793	20	diseases	disease	NNS
cord-014597-66vd2mdu	793	21	.	.	.
cord-014597-66vd2mdu	794	1	Potential	potential	JJ
cord-014597-66vd2mdu	794	2	fields	field	NNS
cord-014597-66vd2mdu	794	3	for	for	IN
cord-014597-66vd2mdu	794	4	applications	application	NNS
cord-014597-66vd2mdu	794	5	are	be	VBP
cord-014597-66vd2mdu	794	6	discovered	discover	VBN
cord-014597-66vd2mdu	794	7	with	with	IN
cord-014597-66vd2mdu	794	8	increasing	increase	VBG
cord-014597-66vd2mdu	794	9	knowledge	knowledge	NN
cord-014597-66vd2mdu	794	10	about	about	IN
cord-014597-66vd2mdu	794	11	these	these	DT
cord-014597-66vd2mdu	794	12	molecules	molecule	NNS
cord-014597-66vd2mdu	794	13	.	.	.
cord-014597-66vd2mdu	795	1	It	-PRON-	PRP
cord-014597-66vd2mdu	795	2	seems	seem	VBZ
cord-014597-66vd2mdu	795	3	that	that	IN
cord-014597-66vd2mdu	795	4	the	the	DT
cord-014597-66vd2mdu	795	5	most	most	RBS
cord-014597-66vd2mdu	795	6	active	active	JJ
cord-014597-66vd2mdu	795	7	forms	form	NNS
cord-014597-66vd2mdu	795	8	are	be	VBP
cord-014597-66vd2mdu	795	9	pentameric	pentameric	JJ
cord-014597-66vd2mdu	795	10	and	and	CC
cord-014597-66vd2mdu	795	11	hexameric	hexameric	JJ
cord-014597-66vd2mdu	795	12	IgMs	IgMs	''
cord-014597-66vd2mdu	795	13	.	.	.
cord-014597-66vd2mdu	796	1	Unfortunately	unfortunately	RB
cord-014597-66vd2mdu	796	2	,	,	,
cord-014597-66vd2mdu	796	3	recombinant	recombinant	JJ
cord-014597-66vd2mdu	796	4	production	production	NN
cord-014597-66vd2mdu	796	5	of	of	IN
cord-014597-66vd2mdu	796	6	IgMs	IgMs	NNP
cord-014597-66vd2mdu	796	7	is	be	VBZ
cord-014597-66vd2mdu	796	8	rather	rather	RB
cord-014597-66vd2mdu	796	9	difficult	difficult	JJ
cord-014597-66vd2mdu	796	10	as	as	IN
cord-014597-66vd2mdu	796	11	secretion	secretion	NN
cord-014597-66vd2mdu	796	12	and	and	CC
cord-014597-66vd2mdu	796	13	correct	correct	JJ
cord-014597-66vd2mdu	796	14	polymer	polymer	NN
cord-014597-66vd2mdu	796	15	formation	formation	NN
cord-014597-66vd2mdu	796	16	results	result	NNS
cord-014597-66vd2mdu	796	17	in	in	IN
cord-014597-66vd2mdu	796	18	low	low	JJ
cord-014597-66vd2mdu	796	19	expression	expression	NN
cord-014597-66vd2mdu	796	20	yields	yield	NNS
cord-014597-66vd2mdu	796	21	and	and	CC
cord-014597-66vd2mdu	796	22	mixtures	mixture	NNS
cord-014597-66vd2mdu	796	23	of	of	IN
cord-014597-66vd2mdu	796	24	polymers	polymer	NNS
cord-014597-66vd2mdu	796	25	.	.	.
cord-014597-66vd2mdu	797	1	We	-PRON-	PRP
cord-014597-66vd2mdu	797	2	established	establish	VBD
cord-014597-66vd2mdu	797	3	stable	stable	JJ
cord-014597-66vd2mdu	797	4	producing	produce	VBG
cord-014597-66vd2mdu	797	5	Chinese	chinese	JJ
cord-014597-66vd2mdu	797	6	hamster	hamster	NN
cord-014597-66vd2mdu	797	7	ovary	ovary	NN
cord-014597-66vd2mdu	798	1	(	(	-LRB-
cord-014597-66vd2mdu	798	2	CHO	CHO	NNP
cord-014597-66vd2mdu	798	3	)	)	-RRB-
cord-014597-66vd2mdu	798	4	DG44	DG44	NNP
cord-014597-66vd2mdu	798	5	cell	cell	NN
cord-014597-66vd2mdu	798	6	lines	line	NNS
cord-014597-66vd2mdu	798	7	to	to	TO
cord-014597-66vd2mdu	798	8	analyze	analyze	VB
cord-014597-66vd2mdu	798	9	cellular	cellular	JJ
cord-014597-66vd2mdu	798	10	and	and	CC
cord-014597-66vd2mdu	798	11	extracellular	extracellular	JJ
cord-014597-66vd2mdu	798	12	factors	factor	NNS
cord-014597-66vd2mdu	798	13	that	that	WDT
cord-014597-66vd2mdu	798	14	influence	influence	VBP
cord-014597-66vd2mdu	798	15	quantity	quantity	NN
cord-014597-66vd2mdu	798	16	and	and	CC
cord-014597-66vd2mdu	798	17	quality	quality	NN
cord-014597-66vd2mdu	798	18	of	of	IN
cord-014597-66vd2mdu	798	19	the	the	DT
cord-014597-66vd2mdu	798	20	produced	produce	VBN
cord-014597-66vd2mdu	798	21	recombinant	recombinant	JJ
cord-014597-66vd2mdu	798	22	polymeric	polymeric	JJ
cord-014597-66vd2mdu	798	23	IgM	IgM	NNP
cord-014597-66vd2mdu	798	24	in	in	IN
cord-014597-66vd2mdu	798	25	future	future	JJ
cord-014597-66vd2mdu	798	26	studies	study	NNS
cord-014597-66vd2mdu	798	27	[	[	-LRB-
cord-014597-66vd2mdu	798	28	1	1	CD
cord-014597-66vd2mdu	798	29	]	]	-RRB-
cord-014597-66vd2mdu	798	30	.	.	.
cord-014597-66vd2mdu	799	1	One	one	CD
cord-014597-66vd2mdu	799	2	quality	quality	NN
cord-014597-66vd2mdu	799	3	parameter	parameter	NN
cord-014597-66vd2mdu	799	4	is	be	VBZ
cord-014597-66vd2mdu	799	5	polymer	polymer	NN
cord-014597-66vd2mdu	799	6	distribution	distribution	NN
cord-014597-66vd2mdu	799	7	,	,	,
cord-014597-66vd2mdu	799	8	which	which	WDT
cord-014597-66vd2mdu	799	9	can	can	MD
cord-014597-66vd2mdu	799	10	be	be	VB
cord-014597-66vd2mdu	799	11	measured	measure	VBN
cord-014597-66vd2mdu	799	12	directly	directly	RB
cord-014597-66vd2mdu	799	13	in	in	IN
cord-014597-66vd2mdu	799	14	cell	cell	NN
cord-014597-66vd2mdu	799	15	culture	culture	NN
cord-014597-66vd2mdu	799	16	supernatant	supernatant	VBN
cord-014597-66vd2mdu	799	17	using	use	VBG
cord-014597-66vd2mdu	799	18	densitometric	densitometric	JJ
cord-014597-66vd2mdu	799	19	analyses	analysis	NNS
cord-014597-66vd2mdu	799	20	[	[	-LRB-
cord-014597-66vd2mdu	799	21	2	2	CD
cord-014597-66vd2mdu	799	22	]	]	-RRB-
cord-014597-66vd2mdu	799	23	.	.	.
cord-014597-66vd2mdu	800	1	Additionally	additionally	RB
cord-014597-66vd2mdu	800	2	,	,	,
cord-014597-66vd2mdu	800	3	we	-PRON-	PRP
cord-014597-66vd2mdu	800	4	developed	develop	VBD
cord-014597-66vd2mdu	800	5	a	a	DT
cord-014597-66vd2mdu	800	6	very	very	RB
cord-014597-66vd2mdu	800	7	efficient	efficient	JJ
cord-014597-66vd2mdu	800	8	single	single	JJ
cord-014597-66vd2mdu	800	9	-	-	HYPH
cord-014597-66vd2mdu	800	10	step	step	NN
cord-014597-66vd2mdu	800	11	-	-	HYPH
cord-014597-66vd2mdu	800	12	affinity	affinity	NN
cord-014597-66vd2mdu	800	13	purification	purification	NN
cord-014597-66vd2mdu	800	14	strategy	strategy	NN
cord-014597-66vd2mdu	800	15	using	use	VBG
cord-014597-66vd2mdu	800	16	the	the	DT
cord-014597-66vd2mdu	800	17	POROS	POROS	NNP
cord-014597-66vd2mdu	800	18	CaptureSelect	CaptureSelect	NNP
cord-014597-66vd2mdu	800	19	IgM	IgM	NNP
cord-014597-66vd2mdu	800	20	Affinity	Affinity	NNP
cord-014597-66vd2mdu	800	21	Matrix	Matrix	NNP
cord-014597-66vd2mdu	800	22	to	to	TO
cord-014597-66vd2mdu	800	23	analyze	analyze	VB
cord-014597-66vd2mdu	800	24	pure	pure	JJ
cord-014597-66vd2mdu	800	25	IgMs	IgMs	NNP
cord-014597-66vd2mdu	800	26	.	.	.
cord-014597-66vd2mdu	801	1	For	for	IN
cord-014597-66vd2mdu	801	2	more	more	RBR
cord-014597-66vd2mdu	801	3	precise	precise	JJ
cord-014597-66vd2mdu	801	4	measurements	measurement	NNS
cord-014597-66vd2mdu	801	5	of	of	IN
cord-014597-66vd2mdu	801	6	the	the	DT
cord-014597-66vd2mdu	801	7	IgM	IgM	NNP
cord-014597-66vd2mdu	801	8	isoform	isoform	NNP
cord-014597-66vd2mdu	801	9	distribution	distribution	NN
cord-014597-66vd2mdu	801	10	we	-PRON-	PRP
cord-014597-66vd2mdu	801	11	separated	separate	VBD
cord-014597-66vd2mdu	801	12	the	the	DT
cord-014597-66vd2mdu	801	13	purified	purify	VBN
cord-014597-66vd2mdu	801	14	polymers	polymer	NNS
cord-014597-66vd2mdu	801	15	by	by	IN
cord-014597-66vd2mdu	801	16	high	high	JJ
cord-014597-66vd2mdu	801	17	performance	performance	NN
cord-014597-66vd2mdu	801	18	liquid	liquid	NN
cord-014597-66vd2mdu	801	19	size	size	NN
cord-014597-66vd2mdu	801	20	exclusion	exclusion	NN
cord-014597-66vd2mdu	801	21	chromatography	chromatography	NN
cord-014597-66vd2mdu	801	22	(	(	-LRB-
cord-014597-66vd2mdu	801	23	SEC	SEC	NNP
cord-014597-66vd2mdu	801	24	HPLC	HPLC	NNP
cord-014597-66vd2mdu	801	25	)	)	-RRB-
cord-014597-66vd2mdu	801	26	.	.	.
cord-014597-66vd2mdu	802	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	802	2	CHO	CHO	NNP
cord-014597-66vd2mdu	802	3	DG44	DG44	NNP
cord-014597-66vd2mdu	802	4	cell	cell	NN
cord-014597-66vd2mdu	802	5	lines	line	NNS
cord-014597-66vd2mdu	802	6	grow	grow	VBP
cord-014597-66vd2mdu	802	7	to	to	TO
cord-014597-66vd2mdu	802	8	peak	peak	VB
cord-014597-66vd2mdu	802	9	cell	cell	NN
cord-014597-66vd2mdu	802	10	concentration	concentration	NN
cord-014597-66vd2mdu	802	11	of	of	IN
cord-014597-66vd2mdu	802	12	4.5x10	4.5x10	CD
cord-014597-66vd2mdu	802	13	6	6	CD
cord-014597-66vd2mdu	802	14	cells	cell	NNS
cord-014597-66vd2mdu	802	15	/	/	SYM
cord-014597-66vd2mdu	802	16	mL	ml	NN
cord-014597-66vd2mdu	802	17	in	in	IN
cord-014597-66vd2mdu	802	18	Erlenmeyer	Erlenmeyer	NNP
cord-014597-66vd2mdu	802	19	flasks	flask	NNS
cord-014597-66vd2mdu	802	20	and	and	CC
cord-014597-66vd2mdu	802	21	4.0x10	4.0x10	CD
cord-014597-66vd2mdu	802	22	6	6	CD
cord-014597-66vd2mdu	802	23	cells	cell	NNS
cord-014597-66vd2mdu	802	24	/	/	SYM
cord-014597-66vd2mdu	802	25	mL	ml	NN
cord-014597-66vd2mdu	802	26	in	in	IN
cord-014597-66vd2mdu	802	27	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	802	28	.	.	.
cord-014597-66vd2mdu	803	1	Similar	similar	JJ
cord-014597-66vd2mdu	803	2	productivity	productivity	NN
cord-014597-66vd2mdu	803	3	of	of	IN
cord-014597-66vd2mdu	803	4	approximately	approximately	RB
cord-014597-66vd2mdu	803	5	50	50	CD
cord-014597-66vd2mdu	803	6	mg	mg	NNP
cord-014597-66vd2mdu	803	7	/	/	SYM
cord-014597-66vd2mdu	803	8	L	L	NNP
cord-014597-66vd2mdu	803	9	was	be	VBD
cord-014597-66vd2mdu	803	10	observed	observe	VBN
cord-014597-66vd2mdu	803	11	for	for	IN
cord-014597-66vd2mdu	803	12	cells	cell	NNS
cord-014597-66vd2mdu	803	13	cultivated	cultivate	VBN
cord-014597-66vd2mdu	803	14	in	in	IN
cord-014597-66vd2mdu	803	15	both	both	DT
cord-014597-66vd2mdu	803	16	cultivation	cultivation	NN
cord-014597-66vd2mdu	803	17	vessels	vessel	NNS
cord-014597-66vd2mdu	803	18	in	in	IN
cord-014597-66vd2mdu	803	19	a	a	DT
cord-014597-66vd2mdu	803	20	nonoptimized	nonoptimized	JJ
cord-014597-66vd2mdu	803	21	batch	batch	NN
cord-014597-66vd2mdu	803	22	culture	culture	NN
cord-014597-66vd2mdu	803	23	using	use	VBG
cord-014597-66vd2mdu	803	24	chemically	chemically	RB
cord-014597-66vd2mdu	803	25	defined	define	VBN
cord-014597-66vd2mdu	803	26	media	medium	NNS
cord-014597-66vd2mdu	803	27	.	.	.
cord-014597-66vd2mdu	804	1	Analysing	analyse	VBG
cord-014597-66vd2mdu	804	2	how	how	WRB
cord-014597-66vd2mdu	804	3	cultivation	cultivation	NN
cord-014597-66vd2mdu	804	4	conditions	condition	NNS
cord-014597-66vd2mdu	804	5	affect	affect	VBP
cord-014597-66vd2mdu	804	6	the	the	DT
cord-014597-66vd2mdu	804	7	fraction	fraction	NN
cord-014597-66vd2mdu	804	8	of	of	IN
cord-014597-66vd2mdu	804	9	polymers	polymer	NNS
cord-014597-66vd2mdu	804	10	may	may	MD
cord-014597-66vd2mdu	804	11	offer	offer	VB
cord-014597-66vd2mdu	804	12	clues	clue	NNS
cord-014597-66vd2mdu	804	13	about	about	IN
cord-014597-66vd2mdu	804	14	the	the	DT
cord-014597-66vd2mdu	804	15	assembly	assembly	NN
cord-014597-66vd2mdu	804	16	of	of	IN
cord-014597-66vd2mdu	804	17	polymers	polymer	NNS
cord-014597-66vd2mdu	804	18	and	and	CC
cord-014597-66vd2mdu	804	19	the	the	DT
cord-014597-66vd2mdu	804	20	challenges	challenge	NNS
cord-014597-66vd2mdu	804	21	of	of	IN
cord-014597-66vd2mdu	804	22	IgM	IgM	NNP
cord-014597-66vd2mdu	804	23	production	production	NN
cord-014597-66vd2mdu	804	24	.	.	.
cord-014597-66vd2mdu	805	1	We	-PRON-	PRP
cord-014597-66vd2mdu	805	2	quantified	quantify	VBD
cord-014597-66vd2mdu	805	3	polymeric	polymeric	JJ
cord-014597-66vd2mdu	805	4	distribution	distribution	NN
cord-014597-66vd2mdu	805	5	of	of	IN
cord-014597-66vd2mdu	805	6	IgM	IgM	NNP
cord-014597-66vd2mdu	805	7	directly	directly	RB
cord-014597-66vd2mdu	805	8	in	in	IN
cord-014597-66vd2mdu	805	9	the	the	DT
cord-014597-66vd2mdu	805	10	supernatant	supernatant	NN
cord-014597-66vd2mdu	805	11	using	use	VBG
cord-014597-66vd2mdu	805	12	a	a	DT
cord-014597-66vd2mdu	805	13	densitometric	densitometric	JJ
cord-014597-66vd2mdu	805	14	method	method	NN
cord-014597-66vd2mdu	805	15	[	[	-LRB-
cord-014597-66vd2mdu	805	16	2	2	CD
cord-014597-66vd2mdu	805	17	]	]	-RRB-
cord-014597-66vd2mdu	805	18	.	.	.
cord-014597-66vd2mdu	806	1	Cultivated	cultivate	VBN
cord-014597-66vd2mdu	806	2	under	under	IN
cord-014597-66vd2mdu	806	3	standard	standard	JJ
cord-014597-66vd2mdu	806	4	conditions	condition	NNS
cord-014597-66vd2mdu	806	5	(	(	-LRB-
cord-014597-66vd2mdu	806	6	37	37	CD
cord-014597-66vd2mdu	806	7	°	°	NN
cord-014597-66vd2mdu	806	8	C	c	NN
cord-014597-66vd2mdu	806	9	,	,	,
cord-014597-66vd2mdu	806	10	pH	pH	NNP
cord-014597-66vd2mdu	806	11	7	7	CD
cord-014597-66vd2mdu	806	12	)	)	-RRB-
cord-014597-66vd2mdu	807	1	IgM012	IgM012	NNP
cord-014597-66vd2mdu	807	2	is	be	VBZ
cord-014597-66vd2mdu	807	3	produced	produce	VBN
cord-014597-66vd2mdu	807	4	as	as	IN
cord-014597-66vd2mdu	807	5	90	90	CD
cord-014597-66vd2mdu	807	6	%	%	NN
cord-014597-66vd2mdu	807	7	pentamers	pentamer	NNS
cord-014597-66vd2mdu	807	8	,	,	,
cord-014597-66vd2mdu	807	9	whereas	whereas	IN
cord-014597-66vd2mdu	807	10	IgM012_GL	IgM012_GL	NNP
cord-014597-66vd2mdu	807	11	only	only	RB
cord-014597-66vd2mdu	807	12	consists	consist	VBZ
cord-014597-66vd2mdu	807	13	of	of	IN
cord-014597-66vd2mdu	807	14	approximately	approximately	RB
cord-014597-66vd2mdu	807	15	80	80	CD
cord-014597-66vd2mdu	807	16	%	%	NN
cord-014597-66vd2mdu	807	17	pentamers	pentamer	NNS
cord-014597-66vd2mdu	807	18	.	.	.
cord-014597-66vd2mdu	808	1	The	the	DT
cord-014597-66vd2mdu	808	2	purified	purify	VBN
cord-014597-66vd2mdu	808	3	IgM012_GL	IgM012_GL	NNP
cord-014597-66vd2mdu	808	4	was	be	VBD
cord-014597-66vd2mdu	808	5	analysed	analyse	VBN
cord-014597-66vd2mdu	808	6	with	with	IN
cord-014597-66vd2mdu	808	7	SEC	SEC	NNP
cord-014597-66vd2mdu	808	8	-	-	HYPH
cord-014597-66vd2mdu	808	9	HPLC	HPLC	NNP
cord-014597-66vd2mdu	808	10	and	and	CC
cord-014597-66vd2mdu	808	11	contained	contain	VBD
cord-014597-66vd2mdu	808	12	81	81	CD
cord-014597-66vd2mdu	808	13	%	%	NN
cord-014597-66vd2mdu	808	14	pentamer	pentamer	NN
cord-014597-66vd2mdu	808	15	and	and	CC
cord-014597-66vd2mdu	808	16	19	19	CD
cord-014597-66vd2mdu	808	17	%	%	NN
cord-014597-66vd2mdu	808	18	dimer	dimer	NN
cord-014597-66vd2mdu	808	19	,	,	,
cord-014597-66vd2mdu	808	20	which	which	WDT
cord-014597-66vd2mdu	808	21	is	be	VBZ
cord-014597-66vd2mdu	808	22	comparable	comparable	JJ
cord-014597-66vd2mdu	808	23	to	to	IN
cord-014597-66vd2mdu	808	24	the	the	DT
cord-014597-66vd2mdu	808	25	results	result	NNS
cord-014597-66vd2mdu	808	26	achieved	achieve	VBN
cord-014597-66vd2mdu	808	27	with	with	IN
cord-014597-66vd2mdu	808	28	densitometry	densitometry	NNP
cord-014597-66vd2mdu	808	29	.	.	.
cord-014597-66vd2mdu	809	1	The	the	DT
cord-014597-66vd2mdu	809	2	purification	purification	NN
cord-014597-66vd2mdu	809	3	of	of	IN
cord-014597-66vd2mdu	809	4	the	the	DT
cord-014597-66vd2mdu	809	5	IgM	IgM	NNP
cord-014597-66vd2mdu	809	6	antibodies	antibody	NNS
cord-014597-66vd2mdu	809	7	was	be	VBD
cord-014597-66vd2mdu	809	8	quite	quite	RB
cord-014597-66vd2mdu	809	9	challenging	challenging	JJ
cord-014597-66vd2mdu	809	10	as	as	IN
cord-014597-66vd2mdu	809	11	the	the	DT
cord-014597-66vd2mdu	809	12	manufacturer	manufacturer	NN
cord-014597-66vd2mdu	809	13	recommend	recommend	VBP
cord-014597-66vd2mdu	809	14	acidic	acidic	JJ
cord-014597-66vd2mdu	809	15	elution	elution	NN
cord-014597-66vd2mdu	809	16	,	,	,
cord-014597-66vd2mdu	809	17	which	which	WDT
cord-014597-66vd2mdu	809	18	led	lead	VBD
cord-014597-66vd2mdu	809	19	to	to	IN
cord-014597-66vd2mdu	809	20	aggregation	aggregation	NN
cord-014597-66vd2mdu	809	21	and	and	CC
cord-014597-66vd2mdu	809	22	inefficient	inefficient	JJ
cord-014597-66vd2mdu	809	23	elution	elution	NN
cord-014597-66vd2mdu	809	24	of	of	IN
cord-014597-66vd2mdu	809	25	our	-PRON-	PRP$
cord-014597-66vd2mdu	809	26	model	model	NN
cord-014597-66vd2mdu	809	27	IgMs	IgMs	NNP
cord-014597-66vd2mdu	809	28	.	.	.
cord-014597-66vd2mdu	810	1	Therefore	therefore	RB
cord-014597-66vd2mdu	810	2	,	,	,
cord-014597-66vd2mdu	810	3	we	-PRON-	PRP
cord-014597-66vd2mdu	810	4	screened	screen	VBD
cord-014597-66vd2mdu	810	5	for	for	IN
cord-014597-66vd2mdu	810	6	different	different	JJ
cord-014597-66vd2mdu	810	7	elution	elution	NN
cord-014597-66vd2mdu	810	8	buffers	buffer	NNS
cord-014597-66vd2mdu	810	9	that	that	WDT
cord-014597-66vd2mdu	810	10	prevent	prevent	VBP
cord-014597-66vd2mdu	810	11	denaturation	denaturation	NN
cord-014597-66vd2mdu	810	12	and	and	CC
cord-014597-66vd2mdu	810	13	aggregation	aggregation	NN
cord-014597-66vd2mdu	810	14	.	.	.
cord-014597-66vd2mdu	811	1	By	by	IN
cord-014597-66vd2mdu	811	2	combining	combine	VBG
cord-014597-66vd2mdu	811	3	high	high	JJ
cord-014597-66vd2mdu	811	4	salt	salt	NN
cord-014597-66vd2mdu	811	5	concentrations	concentration	NNS
cord-014597-66vd2mdu	811	6	with	with	IN
cord-014597-66vd2mdu	811	7	moderate	moderate	JJ
cord-014597-66vd2mdu	811	8	pH	pH	NNP
cord-014597-66vd2mdu	811	9	reduction	reduction	NN
cord-014597-66vd2mdu	811	10	we	-PRON-	PRP
cord-014597-66vd2mdu	811	11	optimized	optimize	VBD
cord-014597-66vd2mdu	811	12	elution	elution	NN
cord-014597-66vd2mdu	811	13	conditions	condition	NNS
cord-014597-66vd2mdu	811	14	to	to	IN
cord-014597-66vd2mdu	811	15	88	88	CD
cord-014597-66vd2mdu	811	16	-	-	SYM
cord-014597-66vd2mdu	811	17	99	99	CD
cord-014597-66vd2mdu	811	18	%	%	NN
cord-014597-66vd2mdu	811	19	IgM	IgM	NNP
cord-014597-66vd2mdu	811	20	recovery	recovery	NN
cord-014597-66vd2mdu	811	21	,	,	,
cord-014597-66vd2mdu	811	22	which	which	WDT
cord-014597-66vd2mdu	811	23	corresponded	correspond	VBD
cord-014597-66vd2mdu	811	24	to	to	IN
cord-014597-66vd2mdu	811	25	a	a	DT
cord-014597-66vd2mdu	811	26	five	five	CD
cord-014597-66vd2mdu	811	27	to	to	TO
cord-014597-66vd2mdu	811	28	six	six	CD
cord-014597-66vd2mdu	811	29	fold	fold	JJ
cord-014597-66vd2mdu	811	30	improvement	improvement	NN
cord-014597-66vd2mdu	811	31	compared	compare	VBN
cord-014597-66vd2mdu	811	32	to	to	IN
cord-014597-66vd2mdu	811	33	the	the	DT
cord-014597-66vd2mdu	811	34	manufacturers	manufacturer	NNS
cord-014597-66vd2mdu	811	35	'	'	POS
cord-014597-66vd2mdu	811	36	conditions	condition	NNS
cord-014597-66vd2mdu	811	37	.	.	.
cord-014597-66vd2mdu	812	1	SDS	SDS	NNP
cord-014597-66vd2mdu	812	2	-	-	HYPH
cord-014597-66vd2mdu	812	3	PAGE	PAGE	NNP
cord-014597-66vd2mdu	812	4	analysis	analysis	NN
cord-014597-66vd2mdu	812	5	and	and	CC
cord-014597-66vd2mdu	812	6	SEC	SEC	NNP
cord-014597-66vd2mdu	812	7	-	-	HYPH
cord-014597-66vd2mdu	812	8	HPLC	HPLC	NNP
cord-014597-66vd2mdu	812	9	showed	show	VBD
cord-014597-66vd2mdu	812	10	that	that	IN
cord-014597-66vd2mdu	812	11	our	-PRON-	PRP$
cord-014597-66vd2mdu	812	12	elution	elution	NN
cord-014597-66vd2mdu	812	13	strategy	strategy	NN
cord-014597-66vd2mdu	812	14	resulted	result	VBD
cord-014597-66vd2mdu	812	15	in	in	IN
cord-014597-66vd2mdu	812	16	a	a	DT
cord-014597-66vd2mdu	812	17	very	very	RB
cord-014597-66vd2mdu	812	18	pure	pure	JJ
cord-014597-66vd2mdu	812	19	product	product	NN
cord-014597-66vd2mdu	812	20	after	after	IN
cord-014597-66vd2mdu	812	21	a	a	DT
cord-014597-66vd2mdu	812	22	single	single	JJ
cord-014597-66vd2mdu	812	23	chromatographic	chromatographic	JJ
cord-014597-66vd2mdu	812	24	step	step	NN
cord-014597-66vd2mdu	812	25	.	.	.
cord-014597-66vd2mdu	813	1	The	the	DT
cord-014597-66vd2mdu	813	2	purification	purification	NN
cord-014597-66vd2mdu	813	3	strategy	strategy	NN
cord-014597-66vd2mdu	813	4	was	be	VBD
cord-014597-66vd2mdu	813	5	verified	verify	VBN
cord-014597-66vd2mdu	813	6	with	with	IN
cord-014597-66vd2mdu	813	7	the	the	DT
cord-014597-66vd2mdu	813	8	IgM103	IgM103	NNP
cord-014597-66vd2mdu	813	9	,	,	,
cord-014597-66vd2mdu	813	10	IgM104	IgM104	NNP
cord-014597-66vd2mdu	813	11	and	and	CC
cord-014597-66vd2mdu	813	12	IgM617	IgM617	NNP
cord-014597-66vd2mdu	813	13	.	.	.
cord-014597-66vd2mdu	814	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	814	2	model	model	NN
cord-014597-66vd2mdu	814	3	IgMs	IgMs	NNP
cord-014597-66vd2mdu	814	4	were	be	VBD
cord-014597-66vd2mdu	814	5	produced	produce	VBN
cord-014597-66vd2mdu	814	6	in	in	IN
cord-014597-66vd2mdu	814	7	a	a	DT
cord-014597-66vd2mdu	814	8	ratio	ratio	NN
cord-014597-66vd2mdu	814	9	of	of	IN
cord-014597-66vd2mdu	814	10	approximately	approximately	RB
cord-014597-66vd2mdu	814	11	4:1	4:1	CD
cord-014597-66vd2mdu	814	12	pentameric	pentameric	NN
cord-014597-66vd2mdu	814	13	to	to	IN
cord-014597-66vd2mdu	814	14	dimeric	dimeric	JJ
cord-014597-66vd2mdu	814	15	IgM	IgM	NNP
cord-014597-66vd2mdu	814	16	,	,	,
cord-014597-66vd2mdu	814	17	measured	measure	VBN
cord-014597-66vd2mdu	814	18	concordantly	concordantly	RB
cord-014597-66vd2mdu	814	19	with	with	IN
cord-014597-66vd2mdu	814	20	both	both	DT
cord-014597-66vd2mdu	814	21	analytical	analytical	JJ
cord-014597-66vd2mdu	814	22	methods	method	NNS
cord-014597-66vd2mdu	814	23	.	.	.
cord-014597-66vd2mdu	815	1	Process	process	NN
cord-014597-66vd2mdu	815	2	development	development	NN
cord-014597-66vd2mdu	815	3	on	on	IN
cord-014597-66vd2mdu	815	4	IgM	IgM	NNP
cord-014597-66vd2mdu	815	5	purification	purification	NN
cord-014597-66vd2mdu	815	6	using	use	VBG
cord-014597-66vd2mdu	815	7	the	the	DT
cord-014597-66vd2mdu	815	8	POROS	POROS	NNP
cord-014597-66vd2mdu	815	9	Capture	Capture	NNP
cord-014597-66vd2mdu	815	10	Select	Select	NNP
cord-014597-66vd2mdu	815	11	human	human	JJ
cord-014597-66vd2mdu	815	12	IgM	IgM	NNP
cord-014597-66vd2mdu	815	13	affinity	affinity	NNP
cord-014597-66vd2mdu	815	14	matrix	matrix	NNP
cord-014597-66vd2mdu	815	15	enabled	enable	VBD
cord-014597-66vd2mdu	815	16	the	the	DT
cord-014597-66vd2mdu	815	17	recovery	recovery	NN
cord-014597-66vd2mdu	815	18	of	of	IN
cord-014597-66vd2mdu	815	19	highly	highly	RB
cord-014597-66vd2mdu	815	20	pure	pure	JJ
cord-014597-66vd2mdu	815	21	fractions	fraction	NNS
cord-014597-66vd2mdu	815	22	.	.	.
cord-014597-66vd2mdu	816	1	Through	through	IN
cord-014597-66vd2mdu	816	2	optimization	optimization	NN
cord-014597-66vd2mdu	816	3	,	,	,
cord-014597-66vd2mdu	816	4	by	by	IN
cord-014597-66vd2mdu	816	5	combining	combine	VBG
cord-014597-66vd2mdu	816	6	mild	mild	JJ
cord-014597-66vd2mdu	816	7	pH	ph	NN
cord-014597-66vd2mdu	816	8	and	and	CC
cord-014597-66vd2mdu	816	9	high	high	JJ
cord-014597-66vd2mdu	816	10	salt	salt	NN
cord-014597-66vd2mdu	816	11	concentrations	concentration	NNS
cord-014597-66vd2mdu	816	12	,	,	,
cord-014597-66vd2mdu	816	13	the	the	DT
cord-014597-66vd2mdu	816	14	relatively	relatively	RB
cord-014597-66vd2mdu	816	15	low	low	JJ
cord-014597-66vd2mdu	816	16	elution	elution	NN
cord-014597-66vd2mdu	816	17	yields	yield	NNS
cord-014597-66vd2mdu	816	18	were	be	VBD
cord-014597-66vd2mdu	816	19	increased	increase	VBN
cord-014597-66vd2mdu	816	20	by	by	IN
cord-014597-66vd2mdu	816	21	a	a	DT
cord-014597-66vd2mdu	816	22	factor	factor	NN
cord-014597-66vd2mdu	816	23	of	of	IN
cord-014597-66vd2mdu	816	24	5	5	CD
cord-014597-66vd2mdu	816	25	-	-	SYM
cord-014597-66vd2mdu	816	26	6	6	CD
cord-014597-66vd2mdu	816	27	.	.	.
cord-014597-66vd2mdu	817	1	Applying	apply	VBG
cord-014597-66vd2mdu	817	2	densitometry	densitometry	NN
cord-014597-66vd2mdu	817	3	and	and	CC
cord-014597-66vd2mdu	817	4	SEC	SEC	NNP
cord-014597-66vd2mdu	817	5	-	-	HYPH
cord-014597-66vd2mdu	817	6	HPLC	HPLC	NNP
cord-014597-66vd2mdu	818	1	we	-PRON-	PRP
cord-014597-66vd2mdu	818	2	will	will	MD
cord-014597-66vd2mdu	818	3	investigate	investigate	VB
cord-014597-66vd2mdu	818	4	how	how	WRB
cord-014597-66vd2mdu	818	5	culture	culture	NN
cord-014597-66vd2mdu	818	6	conditions	condition	NNS
cord-014597-66vd2mdu	818	7	influence	influence	VBP
cord-014597-66vd2mdu	818	8	polymer	polymer	NN
cord-014597-66vd2mdu	818	9	formation	formation	NN
cord-014597-66vd2mdu	818	10	in	in	IN
cord-014597-66vd2mdu	818	11	future	future	NN
cord-014597-66vd2mdu	818	12	.	.	.
cord-014597-66vd2mdu	819	1	Currently	currently	RB
cord-014597-66vd2mdu	819	2	,	,	,
cord-014597-66vd2mdu	819	3	no	no	DT
cord-014597-66vd2mdu	819	4	small	small	JJ
cord-014597-66vd2mdu	819	5	scale	scale	NN
cord-014597-66vd2mdu	819	6	(	(	-LRB-
cord-014597-66vd2mdu	819	7	<	<	XX
cord-014597-66vd2mdu	819	8	0.5L	0.5L	NNS
cord-014597-66vd2mdu	819	9	)	)	-RRB-
cord-014597-66vd2mdu	820	1	cell	cell	NN
cord-014597-66vd2mdu	820	2	culture	culture	NN
cord-014597-66vd2mdu	820	3	system	system	NN
cord-014597-66vd2mdu	820	4	is	be	VBZ
cord-014597-66vd2mdu	820	5	commercially	commercially	RB
cord-014597-66vd2mdu	820	6	available	available	JJ
cord-014597-66vd2mdu	820	7	for	for	IN
cord-014597-66vd2mdu	820	8	high	high	JJ
cord-014597-66vd2mdu	820	9	cell	cell	NN
cord-014597-66vd2mdu	820	10	density	density	NN
cord-014597-66vd2mdu	820	11	perfusion	perfusion	NN
cord-014597-66vd2mdu	820	12	cultivations	cultivation	NNS
cord-014597-66vd2mdu	820	13	to	to	TO
cord-014597-66vd2mdu	820	14	use	use	VB
cord-014597-66vd2mdu	820	15	in	in	IN
cord-014597-66vd2mdu	820	16	high	high	JJ
cord-014597-66vd2mdu	820	17	throughput	throughput	NN
cord-014597-66vd2mdu	820	18	screening	screening	NN
cord-014597-66vd2mdu	820	19	studies	study	NNS
cord-014597-66vd2mdu	820	20	.	.	.
cord-014597-66vd2mdu	821	1	To	to	TO
cord-014597-66vd2mdu	821	2	increase	increase	VB
cord-014597-66vd2mdu	821	3	throughput	throughput	NN
cord-014597-66vd2mdu	821	4	for	for	IN
cord-014597-66vd2mdu	821	5	process	process	NN
cord-014597-66vd2mdu	821	6	characterization	characterization	NN
cord-014597-66vd2mdu	821	7	activities	activity	NNS
cord-014597-66vd2mdu	821	8	at	at	IN
cord-014597-66vd2mdu	821	9	Janssen	Janssen	NNP
cord-014597-66vd2mdu	821	10	Vaccines	Vaccines	NNP
cord-014597-66vd2mdu	821	11	and	and	CC
cord-014597-66vd2mdu	821	12	Prevention	Prevention	NNP
cord-014597-66vd2mdu	821	13	,	,	,
cord-014597-66vd2mdu	821	14	a	a	DT
cord-014597-66vd2mdu	821	15	shaker	shaker	NN
cord-014597-66vd2mdu	821	16	flask	flask	NN
cord-014597-66vd2mdu	821	17	-	-	HYPH
cord-014597-66vd2mdu	821	18	based	base	VBN
cord-014597-66vd2mdu	821	19	scale	scale	NN
cord-014597-66vd2mdu	821	20	down	down	RP
cord-014597-66vd2mdu	821	21	model	model	NN
cord-014597-66vd2mdu	821	22	was	be	VBD
cord-014597-66vd2mdu	821	23	developed	develop	VBN
cord-014597-66vd2mdu	821	24	.	.	.
cord-014597-66vd2mdu	822	1	Though	though	RB
cord-014597-66vd2mdu	822	2	,	,	,
cord-014597-66vd2mdu	822	3	the	the	DT
cord-014597-66vd2mdu	822	4	control	control	NN
cord-014597-66vd2mdu	822	5	possibilities	possibility	NNS
cord-014597-66vd2mdu	822	6	of	of	IN
cord-014597-66vd2mdu	822	7	shaker	shaker	NN
cord-014597-66vd2mdu	822	8	flask	flask	NN
cord-014597-66vd2mdu	822	9	cultures	culture	NNS
cord-014597-66vd2mdu	822	10	are	be	VBP
cord-014597-66vd2mdu	822	11	technically	technically	RB
cord-014597-66vd2mdu	822	12	very	very	RB
cord-014597-66vd2mdu	822	13	limited	limited	JJ
cord-014597-66vd2mdu	822	14	and	and	CC
cord-014597-66vd2mdu	822	15	different	different	JJ
cord-014597-66vd2mdu	822	16	compared	compare	VBN
cord-014597-66vd2mdu	822	17	to	to	IN
cord-014597-66vd2mdu	822	18	a	a	DT
cord-014597-66vd2mdu	822	19	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	822	20	controlled	control	VBN
cord-014597-66vd2mdu	822	21	process	process	NN
cord-014597-66vd2mdu	822	22	.	.	.
cord-014597-66vd2mdu	823	1	In	in	IN
cord-014597-66vd2mdu	823	2	addition	addition	NN
cord-014597-66vd2mdu	823	3	,	,	,
cord-014597-66vd2mdu	823	4	the	the	DT
cord-014597-66vd2mdu	823	5	sensitivity	sensitivity	NN
cord-014597-66vd2mdu	823	6	of	of	IN
cord-014597-66vd2mdu	823	7	the	the	DT
cord-014597-66vd2mdu	823	8	shaker	shaker	NN
cord-014597-66vd2mdu	823	9	flask	flask	NN
cord-014597-66vd2mdu	823	10	model	model	NN
cord-014597-66vd2mdu	823	11	should	should	MD
cord-014597-66vd2mdu	823	12	allow	allow	VB
cord-014597-66vd2mdu	823	13	the	the	DT
cord-014597-66vd2mdu	823	14	detection	detection	NN
cord-014597-66vd2mdu	823	15	of	of	IN
cord-014597-66vd2mdu	823	16	the	the	DT
cord-014597-66vd2mdu	823	17	effects	effect	NNS
cord-014597-66vd2mdu	823	18	of	of	IN
cord-014597-66vd2mdu	823	19	process	process	NN
cord-014597-66vd2mdu	823	20	parameters	parameter	NNS
cord-014597-66vd2mdu	823	21	on	on	IN
cord-014597-66vd2mdu	823	22	critical	critical	JJ
cord-014597-66vd2mdu	823	23	quality	quality	NN
cord-014597-66vd2mdu	823	24	attributes	attribute	NNS
cord-014597-66vd2mdu	823	25	(	(	-LRB-
cord-014597-66vd2mdu	823	26	CQAs	CQAs	NNP
cord-014597-66vd2mdu	823	27	)	)	-RRB-
cord-014597-66vd2mdu	823	28	of	of	IN
cord-014597-66vd2mdu	823	29	the	the	DT
cord-014597-66vd2mdu	823	30	vaccine	vaccine	NN
cord-014597-66vd2mdu	823	31	produced	produce	VBN
cord-014597-66vd2mdu	823	32	at	at	IN
cord-014597-66vd2mdu	823	33	large	large	JJ
cord-014597-66vd2mdu	823	34	scale	scale	NN
cord-014597-66vd2mdu	823	35	.	.	.
cord-014597-66vd2mdu	824	1	Iterative	iterative	JJ
cord-014597-66vd2mdu	824	2	experiments	experiment	NNS
cord-014597-66vd2mdu	824	3	were	be	VBD
cord-014597-66vd2mdu	824	4	performed	perform	VBN
cord-014597-66vd2mdu	824	5	in	in	IN
cord-014597-66vd2mdu	824	6	shake	shake	NN
cord-014597-66vd2mdu	824	7	flasks	flask	NNS
cord-014597-66vd2mdu	824	8	to	to	TO
cord-014597-66vd2mdu	824	9	evaluate	evaluate	VB
cord-014597-66vd2mdu	824	10	the	the	DT
cord-014597-66vd2mdu	824	11	influence	influence	NN
cord-014597-66vd2mdu	824	12	of	of	IN
cord-014597-66vd2mdu	824	13	cultivation	cultivation	NN
cord-014597-66vd2mdu	824	14	parameters	parameter	NNS
cord-014597-66vd2mdu	824	15	such	such	JJ
cord-014597-66vd2mdu	824	16	as	as	IN
cord-014597-66vd2mdu	824	17	shaking	shake	VBG
cord-014597-66vd2mdu	824	18	speed	speed	NN
cord-014597-66vd2mdu	824	19	,	,	,
cord-014597-66vd2mdu	824	20	working	working	NN
cord-014597-66vd2mdu	824	21	volume	volume	NN
cord-014597-66vd2mdu	824	22	,	,	,
cord-014597-66vd2mdu	824	23	CO2	CO2	NNP
cord-014597-66vd2mdu	824	24	%	%	NN
cord-014597-66vd2mdu	824	25	in	in	IN
cord-014597-66vd2mdu	824	26	the	the	DT
cord-014597-66vd2mdu	824	27	incubator	incubator	NN
cord-014597-66vd2mdu	824	28	and	and	CC
cord-014597-66vd2mdu	824	29	daily	daily	JJ
cord-014597-66vd2mdu	824	30	base	base	NN
cord-014597-66vd2mdu	824	31	additions	addition	NNS
cord-014597-66vd2mdu	824	32	on	on	IN
cord-014597-66vd2mdu	824	33	cultivation	cultivation	NN
cord-014597-66vd2mdu	824	34	parameters	parameter	NNS
cord-014597-66vd2mdu	824	35	(	(	-LRB-
cord-014597-66vd2mdu	824	36	as	as	IN
cord-014597-66vd2mdu	824	37	cell	cell	NN
cord-014597-66vd2mdu	824	38	growth	growth	NN
cord-014597-66vd2mdu	824	39	,	,	,
cord-014597-66vd2mdu	824	40	pH	ph	NN
cord-014597-66vd2mdu	824	41	and	and	CC
cord-014597-66vd2mdu	824	42	DO	DO	NNP
cord-014597-66vd2mdu	824	43	)	)	-RRB-
cord-014597-66vd2mdu	824	44	.	.	.
cord-014597-66vd2mdu	825	1	In	in	IN
cord-014597-66vd2mdu	825	2	addition	addition	NN
cord-014597-66vd2mdu	825	3	,	,	,
cord-014597-66vd2mdu	825	4	a	a	DT
cord-014597-66vd2mdu	825	5	medium	medium	JJ
cord-014597-66vd2mdu	825	6	exchange	exchange	NN
cord-014597-66vd2mdu	825	7	was	be	VBD
cord-014597-66vd2mdu	825	8	tested	test	VBN
cord-014597-66vd2mdu	825	9	to	to	TO
cord-014597-66vd2mdu	825	10	mimic	mimic	VB
cord-014597-66vd2mdu	825	11	the	the	DT
cord-014597-66vd2mdu	825	12	perfusion	perfusion	NN
cord-014597-66vd2mdu	825	13	mode	mode	NN
cord-014597-66vd2mdu	825	14	used	use	VBN
cord-014597-66vd2mdu	825	15	in	in	IN
cord-014597-66vd2mdu	825	16	the	the	DT
cord-014597-66vd2mdu	825	17	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	825	18	process	process	NN
cord-014597-66vd2mdu	825	19	.	.	.
cord-014597-66vd2mdu	826	1	The	the	DT
cord-014597-66vd2mdu	826	2	PreSens	PreSens	NNP
cord-014597-66vd2mdu	826	3	shake	shake	NN
cord-014597-66vd2mdu	826	4	flask	flask	NN
cord-014597-66vd2mdu	826	5	reader	reader	NN
cord-014597-66vd2mdu	826	6	was	be	VBD
cord-014597-66vd2mdu	826	7	implemented	implement	VBN
cord-014597-66vd2mdu	826	8	to	to	TO
cord-014597-66vd2mdu	826	9	allow	allow	VB
cord-014597-66vd2mdu	826	10	for	for	IN
cord-014597-66vd2mdu	826	11	pH	ph	NN
cord-014597-66vd2mdu	826	12	and	and	CC
cord-014597-66vd2mdu	826	13	DO	do	VB
cord-014597-66vd2mdu	826	14	monitoring	monitoring	NN
cord-014597-66vd2mdu	826	15	.	.	.
cord-014597-66vd2mdu	827	1	The	the	DT
cord-014597-66vd2mdu	827	2	conditions	condition	NNS
cord-014597-66vd2mdu	827	3	for	for	IN
cord-014597-66vd2mdu	827	4	which	which	WDT
cord-014597-66vd2mdu	827	5	the	the	DT
cord-014597-66vd2mdu	827	6	performance	performance	NN
cord-014597-66vd2mdu	827	7	as	as	IN
cord-014597-66vd2mdu	827	8	reflected	reflect	VBN
cord-014597-66vd2mdu	827	9	in	in	IN
cord-014597-66vd2mdu	827	10	specific	specific	JJ
cord-014597-66vd2mdu	827	11	virus	virus	NN
cord-014597-66vd2mdu	827	12	titer	titer	NN
cord-014597-66vd2mdu	827	13	showed	show	VBD
cord-014597-66vd2mdu	827	14	the	the	DT
cord-014597-66vd2mdu	827	15	best	good	JJS
cord-014597-66vd2mdu	827	16	fit	fit	NN
cord-014597-66vd2mdu	827	17	were	be	VBD
cord-014597-66vd2mdu	827	18	selected	select	VBN
cord-014597-66vd2mdu	827	19	.	.	.
cord-014597-66vd2mdu	828	1	At	at	IN
cord-014597-66vd2mdu	828	2	these	these	DT
cord-014597-66vd2mdu	828	3	conditions	condition	NNS
cord-014597-66vd2mdu	828	4	,	,	,
cord-014597-66vd2mdu	828	5	a	a	DT
cord-014597-66vd2mdu	828	6	series	series	NN
cord-014597-66vd2mdu	828	7	of	of	IN
cord-014597-66vd2mdu	828	8	parallel	parallel	JJ
cord-014597-66vd2mdu	828	9	shaker	shaker	NN
cord-014597-66vd2mdu	828	10	flask	flask	NN
cord-014597-66vd2mdu	828	11	infections	infection	NNS
cord-014597-66vd2mdu	828	12	were	be	VBD
cord-014597-66vd2mdu	828	13	conducted	conduct	VBN
cord-014597-66vd2mdu	828	14	to	to	TO
cord-014597-66vd2mdu	828	15	demonstrate	demonstrate	VB
cord-014597-66vd2mdu	828	16	statistical	statistical	JJ
cord-014597-66vd2mdu	828	17	equivalence	equivalence	NN
cord-014597-66vd2mdu	828	18	of	of	IN
cord-014597-66vd2mdu	828	19	performance	performance	NN
cord-014597-66vd2mdu	828	20	parameter	parameter	NN
cord-014597-66vd2mdu	828	21	and	and	CC
cord-014597-66vd2mdu	828	22	CQAs	cqa	NNS
cord-014597-66vd2mdu	828	23	(	(	-LRB-
cord-014597-66vd2mdu	828	24	as	as	IN
cord-014597-66vd2mdu	828	25	cell	cell	NN
cord-014597-66vd2mdu	828	26	specific	specific	JJ
cord-014597-66vd2mdu	828	27	IU	IU	NNP
cord-014597-66vd2mdu	828	28	titer	titer	NN
cord-014597-66vd2mdu	828	29	and	and	CC
cord-014597-66vd2mdu	828	30	VP	VP	NNP
cord-014597-66vd2mdu	828	31	/	/	SYM
cord-014597-66vd2mdu	828	32	IU	IU	NNP
cord-014597-66vd2mdu	828	33	ratio	ratio	NN
cord-014597-66vd2mdu	828	34	)	)	-RRB-
cord-014597-66vd2mdu	828	35	between	between	IN
cord-014597-66vd2mdu	828	36	the	the	DT
cord-014597-66vd2mdu	828	37	production	production	NN
cord-014597-66vd2mdu	828	38	scale	scale	NN
cord-014597-66vd2mdu	828	39	and	and	CC
cord-014597-66vd2mdu	828	40	reduced	reduce	VBN
cord-014597-66vd2mdu	828	41	scale	scale	NN
cord-014597-66vd2mdu	828	42	processes	process	NNS
cord-014597-66vd2mdu	828	43	and	and	CC
cord-014597-66vd2mdu	828	44	thus	thus	RB
cord-014597-66vd2mdu	828	45	to	to	TO
cord-014597-66vd2mdu	828	46	qualify	qualify	VB
cord-014597-66vd2mdu	828	47	the	the	DT
cord-014597-66vd2mdu	828	48	shake	shake	NN
cord-014597-66vd2mdu	828	49	flask	flask	NN
cord-014597-66vd2mdu	828	50	as	as	IN
cord-014597-66vd2mdu	828	51	a	a	DT
cord-014597-66vd2mdu	828	52	scale	scale	NN
cord-014597-66vd2mdu	828	53	-	-	HYPH
cord-014597-66vd2mdu	828	54	down	down	RP
cord-014597-66vd2mdu	828	55	model	model	NN
cord-014597-66vd2mdu	828	56	.	.	.
cord-014597-66vd2mdu	829	1	A	a	DT
cord-014597-66vd2mdu	829	2	daily	daily	JJ
cord-014597-66vd2mdu	829	3	medium	medium	JJ
cord-014597-66vd2mdu	829	4	exchange	exchange	NN
cord-014597-66vd2mdu	829	5	by	by	IN
cord-014597-66vd2mdu	829	6	centrifugation	centrifugation	NN
cord-014597-66vd2mdu	829	7	was	be	VBD
cord-014597-66vd2mdu	829	8	implemented	implement	VBN
cord-014597-66vd2mdu	829	9	and	and	CC
cord-014597-66vd2mdu	829	10	cultivation	cultivation	NN
cord-014597-66vd2mdu	829	11	parameters	parameter	NNS
cord-014597-66vd2mdu	829	12	for	for	IN
cord-014597-66vd2mdu	829	13	shake	shake	NN
cord-014597-66vd2mdu	829	14	flasks	flask	NNS
cord-014597-66vd2mdu	829	15	were	be	VBD
cord-014597-66vd2mdu	829	16	identified	identify	VBN
cord-014597-66vd2mdu	829	17	.	.	.
cord-014597-66vd2mdu	830	1	Based	base	VBN
cord-014597-66vd2mdu	830	2	on	on	IN
cord-014597-66vd2mdu	830	3	performance	performance	NN
cord-014597-66vd2mdu	830	4	parameter	parameter	NN
cord-014597-66vd2mdu	830	5	(	(	-LRB-
cord-014597-66vd2mdu	830	6	cell	cell	NN
cord-014597-66vd2mdu	830	7	specific	specific	JJ
cord-014597-66vd2mdu	830	8	VP	VP	NNP
cord-014597-66vd2mdu	830	9	titer	titer	NN
cord-014597-66vd2mdu	830	10	)	)	-RRB-
cord-014597-66vd2mdu	830	11	and	and	CC
cord-014597-66vd2mdu	830	12	the	the	DT
cord-014597-66vd2mdu	830	13	CQAs	cqa	NNS
cord-014597-66vd2mdu	830	14	of	of	IN
cord-014597-66vd2mdu	830	15	the	the	DT
cord-014597-66vd2mdu	830	16	vaccine	vaccine	NN
cord-014597-66vd2mdu	830	17	(	(	-LRB-
cord-014597-66vd2mdu	830	18	cell	cell	NN
cord-014597-66vd2mdu	830	19	specific	specific	JJ
cord-014597-66vd2mdu	830	20	IU	IU	NNP
cord-014597-66vd2mdu	830	21	titer	titer	NN
cord-014597-66vd2mdu	830	22	and	and	CC
cord-014597-66vd2mdu	830	23	VP	VP	NNP
cord-014597-66vd2mdu	830	24	/	/	SYM
cord-014597-66vd2mdu	830	25	IU	IU	NNP
cord-014597-66vd2mdu	830	26	ratio	ratio	NN
cord-014597-66vd2mdu	830	27	)	)	-RRB-
cord-014597-66vd2mdu	830	28	,	,	,
cord-014597-66vd2mdu	830	29	equivalence	equivalence	NN
cord-014597-66vd2mdu	830	30	between	between	IN
cord-014597-66vd2mdu	830	31	the	the	DT
cord-014597-66vd2mdu	830	32	production	production	NN
cord-014597-66vd2mdu	830	33	-	-	HYPH
cord-014597-66vd2mdu	830	34	scale	scale	NN
cord-014597-66vd2mdu	830	35	and	and	CC
cord-014597-66vd2mdu	830	36	scale	scale	VB
cord-014597-66vd2mdu	830	37	down	down	RP
cord-014597-66vd2mdu	830	38	systems	system	NNS
cord-014597-66vd2mdu	830	39	was	be	VBD
cord-014597-66vd2mdu	830	40	confirmed	confirm	VBN
cord-014597-66vd2mdu	830	41	.	.	.
cord-014597-66vd2mdu	831	1	The	the	DT
cord-014597-66vd2mdu	831	2	scale	scale	NN
cord-014597-66vd2mdu	831	3	down	down	RP
cord-014597-66vd2mdu	831	4	model	model	NN
cord-014597-66vd2mdu	831	5	data	datum	NNS
cord-014597-66vd2mdu	831	6	fall	fall	NN
cord-014597-66vd2mdu	831	7	into	into	IN
cord-014597-66vd2mdu	831	8	the	the	DT
cord-014597-66vd2mdu	831	9	95	95	CD
cord-014597-66vd2mdu	831	10	%	%	NN
cord-014597-66vd2mdu	831	11	prediction	prediction	NN
cord-014597-66vd2mdu	831	12	intervals	interval	NNS
cord-014597-66vd2mdu	831	13	calculated	calculate	VBN
cord-014597-66vd2mdu	831	14	on	on	IN
cord-014597-66vd2mdu	831	15	manufacturing	manufacture	VBG
cord-014597-66vd2mdu	831	16	data	datum	NNS
cord-014597-66vd2mdu	831	17	whereas	whereas	IN
cord-014597-66vd2mdu	831	18	scale	scale	VBP
cord-014597-66vd2mdu	831	19	down	down	RP
cord-014597-66vd2mdu	831	20	model	model	NN
cord-014597-66vd2mdu	831	21	data	datum	NNS
cord-014597-66vd2mdu	831	22	from	from	IN
cord-014597-66vd2mdu	831	23	batch	batch	NN
cord-014597-66vd2mdu	831	24	mode	mode	NN
cord-014597-66vd2mdu	831	25	experiments	experiment	NNS
cord-014597-66vd2mdu	831	26	(	(	-LRB-
cord-014597-66vd2mdu	831	27	using	use	VBG
cord-014597-66vd2mdu	831	28	non	non	JJ
cord-014597-66vd2mdu	831	29	optimized	optimized	JJ
cord-014597-66vd2mdu	831	30	cultivation	cultivation	NN
cord-014597-66vd2mdu	831	31	conditions	condition	NNS
cord-014597-66vd2mdu	831	32	)	)	-RRB-
cord-014597-66vd2mdu	831	33	do	do	VBP
cord-014597-66vd2mdu	831	34	not	not	RB
cord-014597-66vd2mdu	831	35	.	.	.
cord-014597-66vd2mdu	832	1	The	the	DT
cord-014597-66vd2mdu	832	2	shaker	shaker	NN
cord-014597-66vd2mdu	832	3	flask	flask	NN
cord-014597-66vd2mdu	832	4	as	as	IN
cord-014597-66vd2mdu	832	5	a	a	DT
cord-014597-66vd2mdu	832	6	scale	scale	NN
cord-014597-66vd2mdu	832	7	down	down	RP
cord-014597-66vd2mdu	832	8	model	model	NN
cord-014597-66vd2mdu	832	9	for	for	IN
cord-014597-66vd2mdu	832	10	the	the	DT
cord-014597-66vd2mdu	832	11	10L	10l	JJ
cord-014597-66vd2mdu	832	12	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	832	13	perfusion	perfusion	NN
cord-014597-66vd2mdu	832	14	process	process	NN
cord-014597-66vd2mdu	832	15	was	be	VBD
cord-014597-66vd2mdu	832	16	qualified	qualified	JJ
cord-014597-66vd2mdu	832	17	.	.	.
cord-014597-66vd2mdu	833	1	This	this	DT
cord-014597-66vd2mdu	833	2	model	model	NN
cord-014597-66vd2mdu	833	3	is	be	VBZ
cord-014597-66vd2mdu	833	4	a	a	DT
cord-014597-66vd2mdu	833	5	tool	tool	NN
cord-014597-66vd2mdu	833	6	to	to	TO
cord-014597-66vd2mdu	833	7	screen	screen	VB
cord-014597-66vd2mdu	833	8	a	a	DT
cord-014597-66vd2mdu	833	9	subset	subset	NN
cord-014597-66vd2mdu	833	10	of	of	IN
cord-014597-66vd2mdu	833	11	process	process	NN
cord-014597-66vd2mdu	833	12	parameters	parameter	NNS
cord-014597-66vd2mdu	833	13	at	at	IN
cord-014597-66vd2mdu	833	14	a	a	DT
cord-014597-66vd2mdu	833	15	higher	high	JJR
cord-014597-66vd2mdu	833	16	throughput	throughput	NN
cord-014597-66vd2mdu	833	17	,	,	,
cord-014597-66vd2mdu	833	18	thereby	thereby	RB
cord-014597-66vd2mdu	833	19	reducing	reduce	VBG
cord-014597-66vd2mdu	833	20	process	process	NN
cord-014597-66vd2mdu	833	21	characterization	characterization	NN
cord-014597-66vd2mdu	833	22	timelines	timeline	NNS
cord-014597-66vd2mdu	833	23	.	.	.
cord-014597-66vd2mdu	834	1	Background	background	NN
cord-014597-66vd2mdu	834	2	Until	until	IN
cord-014597-66vd2mdu	834	3	today	today	NN
cord-014597-66vd2mdu	834	4	,	,	,
cord-014597-66vd2mdu	834	5	the	the	DT
cord-014597-66vd2mdu	834	6	market	market	NN
cord-014597-66vd2mdu	834	7	for	for	IN
cord-014597-66vd2mdu	834	8	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	834	9	proteins	protein	NNS
cord-014597-66vd2mdu	834	10	,	,	,
cord-014597-66vd2mdu	834	11	especially	especially	RB
cord-014597-66vd2mdu	834	12	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	834	13	antibodies	antibody	NNS
cord-014597-66vd2mdu	834	14	,	,	,
cord-014597-66vd2mdu	834	15	is	be	VBZ
cord-014597-66vd2mdu	834	16	gaining	gain	VBG
cord-014597-66vd2mdu	834	17	more	more	JJR
cord-014597-66vd2mdu	834	18	and	and	CC
cord-014597-66vd2mdu	834	19	more	more	JJR
cord-014597-66vd2mdu	834	20	importance	importance	NN
cord-014597-66vd2mdu	834	21	in	in	IN
cord-014597-66vd2mdu	834	22	the	the	DT
cord-014597-66vd2mdu	834	23	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	834	24	field	field	NN
cord-014597-66vd2mdu	834	25	.	.	.
cord-014597-66vd2mdu	835	1	To	to	TO
cord-014597-66vd2mdu	835	2	meet	meet	VB
cord-014597-66vd2mdu	835	3	the	the	DT
cord-014597-66vd2mdu	835	4	increasing	increase	VBG
cord-014597-66vd2mdu	835	5	demand	demand	NN
cord-014597-66vd2mdu	835	6	for	for	IN
cord-014597-66vd2mdu	835	7	these	these	DT
cord-014597-66vd2mdu	835	8	products	product	NNS
cord-014597-66vd2mdu	835	9	,	,	,
cord-014597-66vd2mdu	835	10	the	the	DT
cord-014597-66vd2mdu	835	11	industry	industry	NN
cord-014597-66vd2mdu	835	12	made	make	VBD
cord-014597-66vd2mdu	835	13	tremendous	tremendous	JJ
cord-014597-66vd2mdu	835	14	efforts	effort	NNS
cord-014597-66vd2mdu	835	15	to	to	TO
cord-014597-66vd2mdu	835	16	generate	generate	VB
cord-014597-66vd2mdu	835	17	highly	highly	RB
cord-014597-66vd2mdu	835	18	efficient	efficient	JJ
cord-014597-66vd2mdu	835	19	production	production	NN
cord-014597-66vd2mdu	835	20	systems	system	NNS
cord-014597-66vd2mdu	835	21	.	.	.
cord-014597-66vd2mdu	836	1	One	one	CD
cord-014597-66vd2mdu	836	2	of	of	IN
cord-014597-66vd2mdu	836	3	the	the	DT
cord-014597-66vd2mdu	836	4	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	836	5	industry	industry	NN
cord-014597-66vd2mdu	836	6	's	's	POS
cord-014597-66vd2mdu	836	7	research	research	NN
cord-014597-66vd2mdu	836	8	focuses	focus	VBZ
cord-014597-66vd2mdu	836	9	is	be	VBZ
cord-014597-66vd2mdu	836	10	the	the	DT
cord-014597-66vd2mdu	836	11	improvement	improvement	NN
cord-014597-66vd2mdu	836	12	of	of	IN
cord-014597-66vd2mdu	836	13	the	the	DT
cord-014597-66vd2mdu	836	14	secretion	secretion	NN
cord-014597-66vd2mdu	836	15	process	process	NN
cord-014597-66vd2mdu	836	16	in	in	IN
cord-014597-66vd2mdu	836	17	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	836	18	cells	cell	NNS
cord-014597-66vd2mdu	836	19	.	.	.
cord-014597-66vd2mdu	837	1	In	in	IN
cord-014597-66vd2mdu	837	2	mammalian	mammalian	JJ
cord-014597-66vd2mdu	837	3	cells	cell	NNS
cord-014597-66vd2mdu	837	4	,	,	,
cord-014597-66vd2mdu	837	5	the	the	DT
cord-014597-66vd2mdu	837	6	efficiency	efficiency	NN
cord-014597-66vd2mdu	837	7	of	of	IN
cord-014597-66vd2mdu	837	8	protein	protein	NN
cord-014597-66vd2mdu	837	9	transportation	transportation	NN
cord-014597-66vd2mdu	837	10	strongly	strongly	RB
cord-014597-66vd2mdu	837	11	depends	depend	VBZ
cord-014597-66vd2mdu	837	12	on	on	IN
cord-014597-66vd2mdu	837	13	the	the	DT
cord-014597-66vd2mdu	837	14	translocation	translocation	NN
cord-014597-66vd2mdu	837	15	of	of	IN
cord-014597-66vd2mdu	837	16	a	a	DT
cord-014597-66vd2mdu	837	17	nascent	nascent	JJ
cord-014597-66vd2mdu	837	18	protein	protein	NN
cord-014597-66vd2mdu	837	19	into	into	IN
cord-014597-66vd2mdu	837	20	the	the	DT
cord-014597-66vd2mdu	837	21	ER	er	NN
cord-014597-66vd2mdu	837	22	,	,	,
cord-014597-66vd2mdu	837	23	which	which	WDT
cord-014597-66vd2mdu	837	24	is	be	VBZ
cord-014597-66vd2mdu	837	25	mostly	mostly	RB
cord-014597-66vd2mdu	837	26	conducted	conduct	VBN
cord-014597-66vd2mdu	837	27	by	by	IN
cord-014597-66vd2mdu	837	28	the	the	DT
cord-014597-66vd2mdu	837	29	signal	signal	NN
cord-014597-66vd2mdu	837	30	peptide	peptide	NN
cord-014597-66vd2mdu	837	31	(	(	-LRB-
cord-014597-66vd2mdu	837	32	SP	SP	NNP
cord-014597-66vd2mdu	837	33	)	)	-RRB-
cord-014597-66vd2mdu	837	34	coupled	couple	VBN
cord-014597-66vd2mdu	837	35	to	to	IN
cord-014597-66vd2mdu	837	36	the	the	DT
cord-014597-66vd2mdu	837	37	Nterminus	Nterminus	NNP
cord-014597-66vd2mdu	837	38	.	.	.
cord-014597-66vd2mdu	838	1	Through	through	IN
cord-014597-66vd2mdu	838	2	the	the	DT
cord-014597-66vd2mdu	838	3	interchangeability	interchangeability	NN
cord-014597-66vd2mdu	838	4	of	of	IN
cord-014597-66vd2mdu	838	5	signal	signal	NN
cord-014597-66vd2mdu	838	6	peptides	peptide	NNS
cord-014597-66vd2mdu	838	7	between	between	IN
cord-014597-66vd2mdu	838	8	products	product	NNS
cord-014597-66vd2mdu	838	9	and	and	CC
cord-014597-66vd2mdu	838	10	even	even	RB
cord-014597-66vd2mdu	838	11	species	specie	NNS
cord-014597-66vd2mdu	838	12	,	,	,
cord-014597-66vd2mdu	838	13	a	a	DT
cord-014597-66vd2mdu	838	14	large	large	JJ
cord-014597-66vd2mdu	838	15	variety	variety	NN
cord-014597-66vd2mdu	838	16	can	can	MD
cord-014597-66vd2mdu	838	17	be	be	VB
cord-014597-66vd2mdu	838	18	used	use	VBN
cord-014597-66vd2mdu	838	19	to	to	TO
cord-014597-66vd2mdu	838	20	enhance	enhance	VB
cord-014597-66vd2mdu	838	21	protein	protein	NN
cord-014597-66vd2mdu	838	22	expression	expression	NN
cord-014597-66vd2mdu	838	23	in	in	IN
cord-014597-66vd2mdu	838	24	already	already	RB
cord-014597-66vd2mdu	838	25	existing	exist	VBG
cord-014597-66vd2mdu	838	26	production	production	NN
cord-014597-66vd2mdu	838	27	systems	system	NNS
cord-014597-66vd2mdu	838	28	Materials	material	NNS
cord-014597-66vd2mdu	838	29	and	and	CC
cord-014597-66vd2mdu	838	30	methods	method	NNS
cord-014597-66vd2mdu	838	31	At	at	IN
cord-014597-66vd2mdu	838	32	first	first	RB
cord-014597-66vd2mdu	838	33	the	the	DT
cord-014597-66vd2mdu	838	34	influence	influence	NN
cord-014597-66vd2mdu	838	35	of	of	IN
cord-014597-66vd2mdu	838	36	four	four	CD
cord-014597-66vd2mdu	838	37	different	different	JJ
cord-014597-66vd2mdu	838	38	natural	natural	JJ
cord-014597-66vd2mdu	838	39	SPs	sp	NNS
cord-014597-66vd2mdu	838	40	(	(	-LRB-
cord-014597-66vd2mdu	838	41	SP	SP	NNP
cord-014597-66vd2mdu	838	42	(	(	-LRB-
cord-014597-66vd2mdu	838	43	7	7	CD
cord-014597-66vd2mdu	838	44	)	)	-RRB-
cord-014597-66vd2mdu	838	45	,	,	,
cord-014597-66vd2mdu	838	46	(	(	-LRB-
cord-014597-66vd2mdu	838	47	8)	8)	NNP
cord-014597-66vd2mdu	838	48	,	,	,
cord-014597-66vd2mdu	838	49	(	(	-LRB-
cord-014597-66vd2mdu	838	50	9	9	CD
cord-014597-66vd2mdu	838	51	)	)	-RRB-
cord-014597-66vd2mdu	838	52	and	and	CC
cord-014597-66vd2mdu	838	53	(	(	-LRB-
cord-014597-66vd2mdu	838	54	10	10	CD
cord-014597-66vd2mdu	838	55	)	)	-RRB-
cord-014597-66vd2mdu	838	56	)	)	-RRB-
cord-014597-66vd2mdu	838	57	was	be	VBD
cord-014597-66vd2mdu	838	58	compared	compare	VBN
cord-014597-66vd2mdu	838	59	on	on	IN
cord-014597-66vd2mdu	838	60	the	the	DT
cord-014597-66vd2mdu	838	61	secreted	secrete	VBN
cord-014597-66vd2mdu	838	62	amount	amount	NN
cord-014597-66vd2mdu	838	63	of	of	IN
cord-014597-66vd2mdu	838	64	an	an	DT
cord-014597-66vd2mdu	838	65	IgG4	igg4	JJ
cord-014597-66vd2mdu	838	66	model	model	NN
cord-014597-66vd2mdu	838	67	antibody	antibody	NN
cord-014597-66vd2mdu	838	68	(	(	-LRB-
cord-014597-66vd2mdu	838	69	product	product	NN
cord-014597-66vd2mdu	838	70	A	a	NN
cord-014597-66vd2mdu	838	71	)	)	-RRB-
cord-014597-66vd2mdu	838	72	in	in	IN
cord-014597-66vd2mdu	838	73	fed	fed	NNP
cord-014597-66vd2mdu	838	74	batches	batch	NNS
cord-014597-66vd2mdu	838	75	using	use	VBG
cord-014597-66vd2mdu	838	76	a	a	DT
cord-014597-66vd2mdu	838	77	CHO	CHO	NNP
cord-014597-66vd2mdu	838	78	DG44	DG44	NNP
cord-014597-66vd2mdu	838	79	host	host	NN
cord-014597-66vd2mdu	838	80	cell	cell	NN
cord-014597-66vd2mdu	838	81	line	line	NN
cord-014597-66vd2mdu	838	82	.	.	.
cord-014597-66vd2mdu	839	1	In	in	IN
cord-014597-66vd2mdu	839	2	the	the	DT
cord-014597-66vd2mdu	839	3	second	second	JJ
cord-014597-66vd2mdu	839	4	part	part	NN
cord-014597-66vd2mdu	839	5	,	,	,
cord-014597-66vd2mdu	839	6	one	one	CD
cord-014597-66vd2mdu	839	7	promising	promising	JJ
cord-014597-66vd2mdu	839	8	SP	sp	NN
cord-014597-66vd2mdu	839	9	-	-	HYPH
cord-014597-66vd2mdu	839	10	candidate	candidate	NN
cord-014597-66vd2mdu	839	11	showing	show	VBG
cord-014597-66vd2mdu	839	12	improved	improve	VBN
cord-014597-66vd2mdu	839	13	secretion	secretion	NN
cord-014597-66vd2mdu	839	14	(	(	-LRB-
cord-014597-66vd2mdu	839	15	SP	SP	NNP
cord-014597-66vd2mdu	839	16	(	(	-LRB-
cord-014597-66vd2mdu	839	17	9	9	CD
cord-014597-66vd2mdu	839	18	)	)	-RRB-
cord-014597-66vd2mdu	839	19	)	)	-RRB-
cord-014597-66vd2mdu	839	20	was	be	VBD
cord-014597-66vd2mdu	839	21	identified	identify	VBN
cord-014597-66vd2mdu	839	22	and	and	CC
cord-014597-66vd2mdu	839	23	the	the	DT
cord-014597-66vd2mdu	839	24	influence	influence	NN
cord-014597-66vd2mdu	839	25	of	of	IN
cord-014597-66vd2mdu	839	26	this	this	DT
cord-014597-66vd2mdu	839	27	SP	SP	NNP
cord-014597-66vd2mdu	839	28	on	on	IN
cord-014597-66vd2mdu	839	29	four	four	CD
cord-014597-66vd2mdu	839	30	additional	additional	JJ
cord-014597-66vd2mdu	839	31	antibody	antibody	NN
cord-014597-66vd2mdu	839	32	products	product	NNS
cord-014597-66vd2mdu	839	33	,	,	,
cord-014597-66vd2mdu	839	34	which	which	WDT
cord-014597-66vd2mdu	839	35	varied	vary	VBD
cord-014597-66vd2mdu	839	36	in	in	IN
cord-014597-66vd2mdu	839	37	their	-PRON-	PRP$
cord-014597-66vd2mdu	839	38	expressability	expressability	NN
cord-014597-66vd2mdu	839	39	from	from	IN
cord-014597-66vd2mdu	839	40	good	good	JJ
cord-014597-66vd2mdu	839	41	to	to	IN
cord-014597-66vd2mdu	839	42	mid	mid	NN
cord-014597-66vd2mdu	839	43	/	/	SYM
cord-014597-66vd2mdu	839	44	bad	bad	JJ
cord-014597-66vd2mdu	839	45	,	,	,
cord-014597-66vd2mdu	839	46	was	be	VBD
cord-014597-66vd2mdu	839	47	investigated	investigate	VBN
cord-014597-66vd2mdu	839	48	.	.	.
cord-014597-66vd2mdu	840	1	In	in	IN
cord-014597-66vd2mdu	840	2	both	both	DT
cord-014597-66vd2mdu	840	3	approaches	approach	NNS
cord-014597-66vd2mdu	840	4	,	,	,
cord-014597-66vd2mdu	840	5	the	the	DT
cord-014597-66vd2mdu	840	6	standard	standard	JJ
cord-014597-66vd2mdu	840	7	SP	SP	NNP
cord-014597-66vd2mdu	840	8	was	be	VBD
cord-014597-66vd2mdu	840	9	implemented	implement	VBN
cord-014597-66vd2mdu	840	10	for	for	IN
cord-014597-66vd2mdu	840	11	comparative	comparative	JJ
cord-014597-66vd2mdu	840	12	reasons	reason	NNS
cord-014597-66vd2mdu	840	13	.	.	.
cord-014597-66vd2mdu	841	1	In	in	IN
cord-014597-66vd2mdu	841	2	the	the	DT
cord-014597-66vd2mdu	841	3	first	first	JJ
cord-014597-66vd2mdu	841	4	approach	approach	NN
cord-014597-66vd2mdu	841	5	,	,	,
cord-014597-66vd2mdu	841	6	four	four	CD
cord-014597-66vd2mdu	841	7	signal	signal	NN
cord-014597-66vd2mdu	841	8	peptides	peptide	NNS
cord-014597-66vd2mdu	841	9	SP	SP	NNP
cord-014597-66vd2mdu	841	10	(	(	-LRB-
cord-014597-66vd2mdu	841	11	7	7	CD
cord-014597-66vd2mdu	841	12	)	)	-RRB-
cord-014597-66vd2mdu	841	13	,	,	,
cord-014597-66vd2mdu	841	14	SP(8	SP(8	NNP
cord-014597-66vd2mdu	841	15	)	)	-RRB-
cord-014597-66vd2mdu	841	16	,	,	,
cord-014597-66vd2mdu	841	17	SP(9	SP(9	NNP
cord-014597-66vd2mdu	841	18	)	)	-RRB-
cord-014597-66vd2mdu	841	19	and	and	CC
cord-014597-66vd2mdu	841	20	SP(10	SP(10	NNP
cord-014597-66vd2mdu	841	21	)	)	-RRB-
cord-014597-66vd2mdu	841	22	were	be	VBD
cord-014597-66vd2mdu	841	23	screened	screen	VBN
cord-014597-66vd2mdu	841	24	for	for	IN
cord-014597-66vd2mdu	841	25	their	-PRON-	PRP$
cord-014597-66vd2mdu	841	26	potential	potential	NN
cord-014597-66vd2mdu	841	27	to	to	TO
cord-014597-66vd2mdu	841	28	improve	improve	VB
cord-014597-66vd2mdu	841	29	the	the	DT
cord-014597-66vd2mdu	841	30	product	product	NN
cord-014597-66vd2mdu	841	31	secretion	secretion	NN
cord-014597-66vd2mdu	841	32	of	of	IN
cord-014597-66vd2mdu	841	33	CHO	CHO	NNP
cord-014597-66vd2mdu	841	34	DG44	DG44	NNP
cord-014597-66vd2mdu	841	35	cells	cell	NNS
cord-014597-66vd2mdu	841	36	expressing	express	VBG
cord-014597-66vd2mdu	841	37	a	a	DT
cord-014597-66vd2mdu	841	38	model	model	NN
cord-014597-66vd2mdu	841	39	antibody	antibody	NN
cord-014597-66vd2mdu	841	40	(	(	-LRB-
cord-014597-66vd2mdu	841	41	product	product	NN
cord-014597-66vd2mdu	841	42	A	a	NN
cord-014597-66vd2mdu	841	43	)	)	-RRB-
cord-014597-66vd2mdu	841	44	.	.	.
cord-014597-66vd2mdu	842	1	The	the	DT
cord-014597-66vd2mdu	842	2	results	result	NNS
cord-014597-66vd2mdu	842	3	revealed	reveal	VBD
cord-014597-66vd2mdu	842	4	a	a	DT
cord-014597-66vd2mdu	842	5	2.4-fold	2.4-fold	CD
cord-014597-66vd2mdu	842	6	increase	increase	NN
cord-014597-66vd2mdu	842	7	in	in	IN
cord-014597-66vd2mdu	842	8	average	average	JJ
cord-014597-66vd2mdu	842	9	final	final	JJ
cord-014597-66vd2mdu	842	10	fed	fed	NNP
cord-014597-66vd2mdu	842	11	-	-	HYPH
cord-014597-66vd2mdu	842	12	batch	batch	NN
cord-014597-66vd2mdu	842	13	antibody	antibody	NN
cord-014597-66vd2mdu	842	14	titer	titer	NN
cord-014597-66vd2mdu	842	15	of	of	IN
cord-014597-66vd2mdu	842	16	SP(9	SP(9	NNP
cord-014597-66vd2mdu	842	17	)	)	-RRB-
cord-014597-66vd2mdu	842	18	when	when	WRB
cord-014597-66vd2mdu	842	19	compared	compare	VBN
cord-014597-66vd2mdu	842	20	to	to	IN
cord-014597-66vd2mdu	842	21	the	the	DT
cord-014597-66vd2mdu	842	22	standard	standard	JJ
cord-014597-66vd2mdu	842	23	SP	SP	NNP
cord-014597-66vd2mdu	842	24	approach	approach	NN
cord-014597-66vd2mdu	842	25	(	(	-LRB-
cord-014597-66vd2mdu	842	26	standard	standard	JJ
cord-014597-66vd2mdu	842	27	SP	SP	NNP
cord-014597-66vd2mdu	842	28	=	=	SYM
cord-014597-66vd2mdu	842	29	0.44	0.44	CD
cord-014597-66vd2mdu	842	30	g	g	NN
cord-014597-66vd2mdu	842	31	/	/	SYM
cord-014597-66vd2mdu	842	32	L	l	NN
cord-014597-66vd2mdu	842	33	;	;	:
cord-014597-66vd2mdu	842	34	SP(9	SP(9	NNP
cord-014597-66vd2mdu	842	35	)	)	-RRB-
cord-014597-66vd2mdu	842	36	=	=	SYM
cord-014597-66vd2mdu	842	37	1.50	1.50	CD
cord-014597-66vd2mdu	842	38	g	g	NN
cord-014597-66vd2mdu	842	39	/	/	SYM
cord-014597-66vd2mdu	842	40	L	L	NNP
cord-014597-66vd2mdu	842	41	)	)	-RRB-
cord-014597-66vd2mdu	842	42	.	.	.
cord-014597-66vd2mdu	843	1	In	in	IN
cord-014597-66vd2mdu	843	2	the	the	DT
cord-014597-66vd2mdu	843	3	second	second	JJ
cord-014597-66vd2mdu	843	4	approach	approach	NN
cord-014597-66vd2mdu	843	5	,	,	,
cord-014597-66vd2mdu	843	6	the	the	DT
cord-014597-66vd2mdu	843	7	enhancing	enhance	VBG
cord-014597-66vd2mdu	843	8	capacity	capacity	NN
cord-014597-66vd2mdu	843	9	of	of	IN
cord-014597-66vd2mdu	843	10	SP(9	sp(9	NN
cord-014597-66vd2mdu	843	11	)	)	-RRB-
cord-014597-66vd2mdu	843	12	on	on	IN
cord-014597-66vd2mdu	843	13	secretion	secretion	NN
cord-014597-66vd2mdu	843	14	of	of	IN
cord-014597-66vd2mdu	843	15	four	four	CD
cord-014597-66vd2mdu	843	16	other	other	JJ
cord-014597-66vd2mdu	843	17	IgG	igg	NN
cord-014597-66vd2mdu	843	18	products	product	NNS
cord-014597-66vd2mdu	843	19	(	(	-LRB-
cord-014597-66vd2mdu	843	20	named	name	VBN
cord-014597-66vd2mdu	843	21	product	product	NN
cord-014597-66vd2mdu	843	22	B	b	NN
cord-014597-66vd2mdu	843	23	to	to	IN
cord-014597-66vd2mdu	843	24	E	e	NN
cord-014597-66vd2mdu	843	25	,	,	,
cord-014597-66vd2mdu	843	26	Table	table	NN
cord-014597-66vd2mdu	843	27	1	1	CD
cord-014597-66vd2mdu	843	28	)	)	-RRB-
cord-014597-66vd2mdu	843	29	was	be	VBD
cord-014597-66vd2mdu	843	30	further	further	RB
cord-014597-66vd2mdu	843	31	evaluated	evaluate	VBN
cord-014597-66vd2mdu	843	32	.	.	.
cord-014597-66vd2mdu	844	1	An	an	DT
cord-014597-66vd2mdu	844	2	improved	improved	JJ
cord-014597-66vd2mdu	844	3	performance	performance	NN
cord-014597-66vd2mdu	844	4	was	be	VBD
cord-014597-66vd2mdu	844	5	observed	observe	VBN
cord-014597-66vd2mdu	844	6	for	for	IN
cord-014597-66vd2mdu	844	7	all	all	DT
cord-014597-66vd2mdu	844	8	products	product	NNS
cord-014597-66vd2mdu	844	9	when	when	WRB
cord-014597-66vd2mdu	844	10	comparing	compare	VBG
cord-014597-66vd2mdu	844	11	SP(9	SP(9	NNP
cord-014597-66vd2mdu	844	12	)	)	-RRB-
cord-014597-66vd2mdu	844	13	and	and	CC
cord-014597-66vd2mdu	844	14	the	the	DT
cord-014597-66vd2mdu	844	15	standard	standard	JJ
cord-014597-66vd2mdu	844	16	SP	SP	NNP
cord-014597-66vd2mdu	844	17	in	in	IN
cord-014597-66vd2mdu	844	18	a	a	DT
cord-014597-66vd2mdu	844	19	fed	fed	NNP
cord-014597-66vd2mdu	844	20	batch	batch	NN
cord-014597-66vd2mdu	844	21	process	process	NN
cord-014597-66vd2mdu	844	22	(	(	-LRB-
cord-014597-66vd2mdu	844	23	Fig	fig	NN
cord-014597-66vd2mdu	844	24	.	.	.
cord-014597-66vd2mdu	844	25	 	 	_SP
cord-014597-66vd2mdu	845	1	1	1	LS
cord-014597-66vd2mdu	845	2	)	)	-RRB-
cord-014597-66vd2mdu	845	3	.	.	.
cord-014597-66vd2mdu	846	1	With	with	IN
cord-014597-66vd2mdu	846	2	an	an	DT
cord-014597-66vd2mdu	846	3	increase	increase	NN
cord-014597-66vd2mdu	846	4	in	in	IN
cord-014597-66vd2mdu	846	5	average	average	JJ
cord-014597-66vd2mdu	846	6	final	final	JJ
cord-014597-66vd2mdu	846	7	fed	fed	NN
cord-014597-66vd2mdu	846	8	-	-	HYPH
cord-014597-66vd2mdu	846	9	batch	batch	NN
cord-014597-66vd2mdu	846	10	titers	titer	NNS
cord-014597-66vd2mdu	846	11	ranging	range	VBG
cord-014597-66vd2mdu	846	12	from	from	IN
cord-014597-66vd2mdu	846	13	28	28	CD
cord-014597-66vd2mdu	846	14	to	to	IN
cord-014597-66vd2mdu	846	15	354	354	CD
cord-014597-66vd2mdu	846	16	%	%	NN
cord-014597-66vd2mdu	846	17	and	and	CC
cord-014597-66vd2mdu	846	18	up	up	IN
cord-014597-66vd2mdu	846	19	to	to	TO
cord-014597-66vd2mdu	846	20	290	290	CD
cord-014597-66vd2mdu	846	21	%	%	NN
cord-014597-66vd2mdu	846	22	in	in	IN
cord-014597-66vd2mdu	846	23	cell	cell	NN
cord-014597-66vd2mdu	846	24	-	-	HYPH
cord-014597-66vd2mdu	846	25	specific	specific	JJ
cord-014597-66vd2mdu	846	26	productivities	productivity	NNS
cord-014597-66vd2mdu	846	27	.	.	.
cord-014597-66vd2mdu	847	1	Taken	take	VBN
cord-014597-66vd2mdu	847	2	together	together	RB
cord-014597-66vd2mdu	847	3	,	,	,
cord-014597-66vd2mdu	847	4	with	with	IN
cord-014597-66vd2mdu	847	5	a	a	DT
cord-014597-66vd2mdu	847	6	positive	positive	JJ
cord-014597-66vd2mdu	847	7	influence	influence	NN
cord-014597-66vd2mdu	847	8	on	on	IN
cord-014597-66vd2mdu	847	9	the	the	DT
cord-014597-66vd2mdu	847	10	final	final	JJ
cord-014597-66vd2mdu	847	11	concentrations	concentration	NNS
cord-014597-66vd2mdu	847	12	of	of	IN
cord-014597-66vd2mdu	847	13	all	all	DT
cord-014597-66vd2mdu	847	14	tested	test	VBN
cord-014597-66vd2mdu	847	15	products	product	NNS
cord-014597-66vd2mdu	847	16	,	,	,
cord-014597-66vd2mdu	847	17	the	the	DT
cord-014597-66vd2mdu	847	18	results	result	NNS
cord-014597-66vd2mdu	847	19	obtained	obtain	VBN
cord-014597-66vd2mdu	847	20	with	with	IN
cord-014597-66vd2mdu	847	21	SP(9	sp(9	NN
cord-014597-66vd2mdu	847	22	)	)	-RRB-
cord-014597-66vd2mdu	847	23	contribute	contribute	VB
cord-014597-66vd2mdu	847	24	to	to	IN
cord-014597-66vd2mdu	847	25	-Signal	-signal	JJ
cord-014597-66vd2mdu	847	26	peptide	peptide	NN
cord-014597-66vd2mdu	847	27	SP(9	SP(9	NNP
cord-014597-66vd2mdu	847	28	)	)	-RRB-
cord-014597-66vd2mdu	847	29	was	be	VBD
cord-014597-66vd2mdu	847	30	identified	identify	VBN
cord-014597-66vd2mdu	847	31	as	as	IN
cord-014597-66vd2mdu	847	32	a	a	DT
cord-014597-66vd2mdu	847	33	promising	promising	JJ
cord-014597-66vd2mdu	847	34	candidate	candidate	NN
cord-014597-66vd2mdu	847	35	with	with	IN
cord-014597-66vd2mdu	847	36	an	an	DT
cord-014597-66vd2mdu	847	37	average	average	JJ
cord-014597-66vd2mdu	847	38	2.4-fold	2.4-fold	CD
cord-014597-66vd2mdu	847	39	titer	titer	NN
cord-014597-66vd2mdu	847	40	increase	increase	NN
cord-014597-66vd2mdu	847	41	during	during	IN
cord-014597-66vd2mdu	847	42	screening	screening	NN
cord-014597-66vd2mdu	847	43	of	of	IN
cord-014597-66vd2mdu	847	44	four	four	CD
cord-014597-66vd2mdu	847	45	signal	signal	NN
cord-014597-66vd2mdu	847	46	peptides	peptide	NNS
cord-014597-66vd2mdu	847	47	.	.	.
cord-014597-66vd2mdu	848	1	-SP(9	-sp(9	LS
cord-014597-66vd2mdu	848	2	)	)	-RRB-
cord-014597-66vd2mdu	848	3	was	be	VBD
cord-014597-66vd2mdu	848	4	able	able	JJ
cord-014597-66vd2mdu	848	5	to	to	TO
cord-014597-66vd2mdu	848	6	improve	improve	VB
cord-014597-66vd2mdu	848	7	production	production	NN
cord-014597-66vd2mdu	848	8	titers	titer	NNS
cord-014597-66vd2mdu	848	9	up	up	RP
cord-014597-66vd2mdu	848	10	to	to	IN
cord-014597-66vd2mdu	848	11	354	354	CD
cord-014597-66vd2mdu	848	12	%	%	NN
cord-014597-66vd2mdu	848	13	compared	compare	VBN
cord-014597-66vd2mdu	848	14	to	to	IN
cord-014597-66vd2mdu	848	15	standard	standard	JJ
cord-014597-66vd2mdu	848	16	SP	SP	NNP
cord-014597-66vd2mdu	848	17	.	.	.
cord-014597-66vd2mdu	849	1	-SP(9	-sp(9	LS
cord-014597-66vd2mdu	849	2	)	)	-RRB-
cord-014597-66vd2mdu	849	3	was	be	VBD
cord-014597-66vd2mdu	849	4	able	able	JJ
cord-014597-66vd2mdu	849	5	to	to	TO
cord-014597-66vd2mdu	849	6	improve	improve	VB
cord-014597-66vd2mdu	849	7	cell	cell	NN
cord-014597-66vd2mdu	849	8	-	-	HYPH
cord-014597-66vd2mdu	849	9	specific	specific	JJ
cord-014597-66vd2mdu	849	10	productivities	productivity	NNS
cord-014597-66vd2mdu	849	11	up	up	IN
cord-014597-66vd2mdu	849	12	to	to	TO
cord-014597-66vd2mdu	849	13	290	290	CD
cord-014597-66vd2mdu	849	14	%	%	NN
cord-014597-66vd2mdu	849	15	compared	compare	VBN
cord-014597-66vd2mdu	849	16	to	to	IN
cord-014597-66vd2mdu	849	17	standard	standard	JJ
cord-014597-66vd2mdu	849	18	SP	SP	NNP
cord-014597-66vd2mdu	849	19	.	.	.
cord-014597-66vd2mdu	850	1	-Future	-Future	:
cord-014597-66vd2mdu	850	2	usage	usage	NN
cord-014597-66vd2mdu	850	3	of	of	IN
cord-014597-66vd2mdu	850	4	SP	SP	NNP
cord-014597-66vd2mdu	850	5	(	(	-LRB-
cord-014597-66vd2mdu	850	6	9	9	CD
cord-014597-66vd2mdu	850	7	)	)	-RRB-
cord-014597-66vd2mdu	850	8	contributes	contribute	VBZ
cord-014597-66vd2mdu	850	9	to	to	IN
cord-014597-66vd2mdu	850	10	the	the	DT
cord-014597-66vd2mdu	850	11	further	further	JJ
cord-014597-66vd2mdu	850	12	optimization	optimization	NN
cord-014597-66vd2mdu	850	13	of	of	IN
cord-014597-66vd2mdu	850	14	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	850	15	Stedim	Stedim	NNP
cord-014597-66vd2mdu	850	16	Cellca	Cellca	NNP
cord-014597-66vd2mdu	850	17	's	's	POS
cord-014597-66vd2mdu	850	18	standard	standard	JJ
cord-014597-66vd2mdu	850	19	cell	cell	NN
cord-014597-66vd2mdu	850	20	line	line	NN
cord-014597-66vd2mdu	850	21	development	development	NN
cord-014597-66vd2mdu	850	22	process	process	NN
cord-014597-66vd2mdu	850	23	.	.	.
cord-014597-66vd2mdu	851	1	New	new	JJ
cord-014597-66vd2mdu	851	2	platform	platform	NN
cord-014597-66vd2mdu	851	3	for	for	IN
cord-014597-66vd2mdu	851	4	the	the	DT
cord-014597-66vd2mdu	851	5	integrated	integrated	JJ
cord-014597-66vd2mdu	851	6	analysis	analysis	NN
cord-014597-66vd2mdu	851	7	of	of	IN
cord-014597-66vd2mdu	851	8	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	851	9	online	online	RB
cord-014597-66vd2mdu	851	10	and	and	CC
cord-014597-66vd2mdu	851	11	offline	offline	JJ
cord-014597-66vd2mdu	851	12	data	datum	NNS
cord-014597-66vd2mdu	851	13	Lukasz	Lukasz	NNP
cord-014597-66vd2mdu	851	14	Gricman	Gricman	NNP
cord-014597-66vd2mdu	851	15	1	1	CD
cord-014597-66vd2mdu	851	16	,	,	,
cord-014597-66vd2mdu	851	17	Milan	Milan	NNP
cord-014597-66vd2mdu	851	18	Ganguly	Ganguly	NNP
cord-014597-66vd2mdu	851	19	2	2	CD
cord-014597-66vd2mdu	851	20	,	,	,
cord-014597-66vd2mdu	851	21	Amanda	Amanda	NNP
cord-014597-66vd2mdu	851	22	Fitzgerald	Fitzgerald	NNP
cord-014597-66vd2mdu	851	23	3	3	CD
cord-014597-66vd2mdu	851	24	,	,	,
cord-014597-66vd2mdu	851	25	Hans	Hans	NNP
cord-014597-66vd2mdu	851	26	Peter	Peter	NNP
cord-014597-66vd2mdu	852	1	More	More	JJR
cord-014597-66vd2mdu	852	2	and	and	CC
cord-014597-66vd2mdu	852	3	more	more	JJR
cord-014597-66vd2mdu	852	4	experiments	experiment	NNS
cord-014597-66vd2mdu	852	5	are	be	VBP
cord-014597-66vd2mdu	852	6	used	use	VBN
cord-014597-66vd2mdu	852	7	to	to	TO
cord-014597-66vd2mdu	852	8	assess	assess	VB
cord-014597-66vd2mdu	852	9	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	852	10	suitability	suitability	NN
cord-014597-66vd2mdu	852	11	and	and	CC
cord-014597-66vd2mdu	852	12	stability	stability	NN
cord-014597-66vd2mdu	852	13	of	of	IN
cord-014597-66vd2mdu	852	14	clones	clone	NNS
cord-014597-66vd2mdu	852	15	,	,	,
cord-014597-66vd2mdu	852	16	to	to	TO
cord-014597-66vd2mdu	852	17	evaluate	evaluate	VB
cord-014597-66vd2mdu	852	18	media	medium	NNS
cord-014597-66vd2mdu	852	19	composition	composition	NN
cord-014597-66vd2mdu	852	20	and	and	CC
cord-014597-66vd2mdu	852	21	other	other	JJ
cord-014597-66vd2mdu	852	22	process	process	NN
cord-014597-66vd2mdu	852	23	parameters	parameter	NNS
cord-014597-66vd2mdu	852	24	,	,	,
cord-014597-66vd2mdu	852	25	and	and	CC
cord-014597-66vd2mdu	852	26	to	to	TO
cord-014597-66vd2mdu	852	27	start	start	VB
cord-014597-66vd2mdu	852	28	upscaling	upscaling	JJ
cord-014597-66vd2mdu	852	29	campaigns	campaign	NNS
cord-014597-66vd2mdu	852	30	.	.	.
cord-014597-66vd2mdu	853	1	This	this	DT
cord-014597-66vd2mdu	853	2	has	have	VBZ
cord-014597-66vd2mdu	853	3	resulted	result	VBN
cord-014597-66vd2mdu	853	4	in	in	IN
cord-014597-66vd2mdu	853	5	a	a	DT
cord-014597-66vd2mdu	853	6	major	major	JJ
cord-014597-66vd2mdu	853	7	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	853	8	due	due	IN
cord-014597-66vd2mdu	853	9	to	to	IN
cord-014597-66vd2mdu	853	10	the	the	DT
cord-014597-66vd2mdu	853	11	increase	increase	NN
cord-014597-66vd2mdu	853	12	in	in	IN
cord-014597-66vd2mdu	853	13	data	datum	NNS
cord-014597-66vd2mdu	853	14	capturing	capture	VBG
cord-014597-66vd2mdu	853	15	,	,	,
cord-014597-66vd2mdu	853	16	processing	processing	NN
cord-014597-66vd2mdu	853	17	,	,	,
cord-014597-66vd2mdu	853	18	aggregation	aggregation	NN
cord-014597-66vd2mdu	853	19	,	,	,
cord-014597-66vd2mdu	853	20	visualization	visualization	NN
cord-014597-66vd2mdu	853	21	,	,	,
cord-014597-66vd2mdu	853	22	and	and	CC
cord-014597-66vd2mdu	853	23	statistical	statistical	JJ
cord-014597-66vd2mdu	853	24	analysis	analysis	NN
cord-014597-66vd2mdu	853	25	.	.	.
cord-014597-66vd2mdu	854	1	In	in	IN
cord-014597-66vd2mdu	854	2	addition	addition	NN
cord-014597-66vd2mdu	854	3	,	,	,
cord-014597-66vd2mdu	854	4	the	the	DT
cord-014597-66vd2mdu	854	5	association	association	NN
cord-014597-66vd2mdu	854	6	of	of	IN
cord-014597-66vd2mdu	854	7	the	the	DT
cord-014597-66vd2mdu	854	8	data	datum	NNS
cord-014597-66vd2mdu	854	9	with	with	IN
cord-014597-66vd2mdu	854	10	the	the	DT
cord-014597-66vd2mdu	854	11	experimental	experimental	JJ
cord-014597-66vd2mdu	854	12	context	context	NN
cord-014597-66vd2mdu	854	13	(	(	-LRB-
cord-014597-66vd2mdu	854	14	e.g.	e.g.	RB
cord-014597-66vd2mdu	854	15	,	,	,
cord-014597-66vd2mdu	854	16	fermentation	fermentation	NN
cord-014597-66vd2mdu	854	17	protocols	protocol	NNS
cord-014597-66vd2mdu	854	18	,	,	,
cord-014597-66vd2mdu	854	19	media	medium	NNS
cord-014597-66vd2mdu	854	20	recipes	recipe	NNS
cord-014597-66vd2mdu	854	21	,	,	,
cord-014597-66vd2mdu	854	22	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	854	23	control	control	NN
cord-014597-66vd2mdu	854	24	parameters	parameter	NNS
cord-014597-66vd2mdu	854	25	)	)	-RRB-
cord-014597-66vd2mdu	854	26	is	be	VBZ
cord-014597-66vd2mdu	854	27	not	not	RB
cord-014597-66vd2mdu	854	28	easily	easily	RB
cord-014597-66vd2mdu	854	29	accomplished	accomplish	VBN
cord-014597-66vd2mdu	854	30	in	in	IN
cord-014597-66vd2mdu	854	31	high	high	JJ
cord-014597-66vd2mdu	854	32	throughput	throughput	NN
cord-014597-66vd2mdu	854	33	.	.	.
cord-014597-66vd2mdu	855	1	The	the	DT
cord-014597-66vd2mdu	855	2	data	datum	NNS
cord-014597-66vd2mdu	855	3	generated	generate	VBN
cord-014597-66vd2mdu	855	4	in	in	IN
cord-014597-66vd2mdu	855	5	the	the	DT
cord-014597-66vd2mdu	855	6	process	process	NN
cord-014597-66vd2mdu	855	7	must	must	MD
cord-014597-66vd2mdu	855	8	not	not	RB
cord-014597-66vd2mdu	855	9	only	only	RB
cord-014597-66vd2mdu	855	10	be	be	VB
cord-014597-66vd2mdu	855	11	analyzed	analyze	VBN
cord-014597-66vd2mdu	855	12	,	,	,
cord-014597-66vd2mdu	855	13	but	but	CC
cord-014597-66vd2mdu	855	14	also	also	RB
cord-014597-66vd2mdu	855	15	managed	manage	VBD
cord-014597-66vd2mdu	855	16	and	and	CC
cord-014597-66vd2mdu	855	17	stored	store	VBN
cord-014597-66vd2mdu	855	18	to	to	TO
cord-014597-66vd2mdu	855	19	enable	enable	VB
cord-014597-66vd2mdu	855	20	easy	easy	JJ
cord-014597-66vd2mdu	855	21	tracking	tracking	NN
cord-014597-66vd2mdu	855	22	and	and	CC
cord-014597-66vd2mdu	855	23	relating	relate	VBG
cord-014597-66vd2mdu	855	24	to	to	IN
cord-014597-66vd2mdu	855	25	historical	historical	JJ
cord-014597-66vd2mdu	855	26	records	record	NNS
cord-014597-66vd2mdu	855	27	.	.	.
cord-014597-66vd2mdu	856	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	856	2	,	,	,
cord-014597-66vd2mdu	856	3	the	the	DT
cord-014597-66vd2mdu	856	4	processes	process	NNS
cord-014597-66vd2mdu	856	5	are	be	VBP
cord-014597-66vd2mdu	856	6	often	often	RB
cord-014597-66vd2mdu	856	7	developed	develop	VBN
cord-014597-66vd2mdu	856	8	by	by	IN
cord-014597-66vd2mdu	856	9	global	global	JJ
cord-014597-66vd2mdu	856	10	teams	team	NNS
cord-014597-66vd2mdu	856	11	interacting	interact	VBG
cord-014597-66vd2mdu	856	12	in	in	IN
cord-014597-66vd2mdu	856	13	complex	complex	JJ
cord-014597-66vd2mdu	856	14	enterprise	enterprise	NN
cord-014597-66vd2mdu	856	15	IT	it	NN
cord-014597-66vd2mdu	856	16	ecosystems	ecosystem	VBZ
cord-014597-66vd2mdu	856	17	.	.	.
cord-014597-66vd2mdu	857	1	Therefore	therefore	RB
cord-014597-66vd2mdu	857	2	,	,	,
cord-014597-66vd2mdu	857	3	new	new	JJ
cord-014597-66vd2mdu	857	4	and	and	CC
cord-014597-66vd2mdu	857	5	high	high	JJ
cord-014597-66vd2mdu	857	6	performing	perform	VBG
cord-014597-66vd2mdu	857	7	systems	system	NNS
cord-014597-66vd2mdu	857	8	for	for	IN
cord-014597-66vd2mdu	857	9	data	datum	NNS
cord-014597-66vd2mdu	857	10	capture	capture	NN
cord-014597-66vd2mdu	857	11	,	,	,
cord-014597-66vd2mdu	857	12	processing	processing	NN
cord-014597-66vd2mdu	857	13	,	,	,
cord-014597-66vd2mdu	857	14	and	and	CC
cord-014597-66vd2mdu	857	15	analysis	analysis	NN
cord-014597-66vd2mdu	857	16	need	need	VBP
cord-014597-66vd2mdu	857	17	to	to	TO
cord-014597-66vd2mdu	857	18	be	be	VB
cord-014597-66vd2mdu	857	19	integrated	integrate	VBN
cord-014597-66vd2mdu	857	20	in	in	IN
cord-014597-66vd2mdu	857	21	order	order	NN
cord-014597-66vd2mdu	857	22	to	to	TO
cord-014597-66vd2mdu	857	23	enable	enable	VB
cord-014597-66vd2mdu	857	24	storage	storage	NN
cord-014597-66vd2mdu	857	25	and	and	CC
cord-014597-66vd2mdu	857	26	correlation	correlation	NN
cord-014597-66vd2mdu	857	27	of	of	IN
cord-014597-66vd2mdu	857	28	experimental	experimental	JJ
cord-014597-66vd2mdu	857	29	context	context	NN
cord-014597-66vd2mdu	857	30	information	information	NN
cord-014597-66vd2mdu	857	31	and	and	CC
cord-014597-66vd2mdu	857	32	various	various	JJ
cord-014597-66vd2mdu	857	33	types	type	NNS
cord-014597-66vd2mdu	857	34	of	of	IN
cord-014597-66vd2mdu	857	35	time	time	NN
cord-014597-66vd2mdu	857	36	course	course	NN
cord-014597-66vd2mdu	857	37	analytics	analytics	NNP
cord-014597-66vd2mdu	857	38	data	datum	NNS
cord-014597-66vd2mdu	857	39	.	.	.
cord-014597-66vd2mdu	858	1	We	-PRON-	PRP
cord-014597-66vd2mdu	858	2	have	have	VBP
cord-014597-66vd2mdu	858	3	developed	develop	VBN
cord-014597-66vd2mdu	858	4	Genedata	Genedata	NNP
cord-014597-66vd2mdu	858	5	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	858	6	™	™	NN
cord-014597-66vd2mdu	858	7	,	,	,
cord-014597-66vd2mdu	858	8	a	a	DT
cord-014597-66vd2mdu	858	9	new	new	JJ
cord-014597-66vd2mdu	858	10	enterprise	enterprise	NN
cord-014597-66vd2mdu	858	11	platform	platform	NN
cord-014597-66vd2mdu	858	12	for	for	IN
cord-014597-66vd2mdu	858	13	bioprocess	bioprocess	NNP
cord-014597-66vd2mdu	858	14	development	development	NN
cord-014597-66vd2mdu	858	15	.	.	.
cord-014597-66vd2mdu	859	1	The	the	DT
cord-014597-66vd2mdu	859	2	platform	platform	NN
cord-014597-66vd2mdu	859	3	enables	enable	VBZ
cord-014597-66vd2mdu	859	4	automatic	automatic	JJ
cord-014597-66vd2mdu	859	5	capture	capture	NN
cord-014597-66vd2mdu	859	6	and	and	CC
cord-014597-66vd2mdu	859	7	visualization	visualization	NN
cord-014597-66vd2mdu	859	8	of	of	IN
cord-014597-66vd2mdu	859	9	all	all	DT
cord-014597-66vd2mdu	859	10	online	online	JJ
cord-014597-66vd2mdu	859	11	and	and	CC
cord-014597-66vd2mdu	859	12	offline	offline	JJ
cord-014597-66vd2mdu	859	13	data	datum	NNS
cord-014597-66vd2mdu	859	14	(	(	-LRB-
cord-014597-66vd2mdu	859	15	e.g.	e.g.	RB
cord-014597-66vd2mdu	859	16	,	,	,
cord-014597-66vd2mdu	859	17	pH	pH	NNP
cord-014597-66vd2mdu	859	18	,	,	,
cord-014597-66vd2mdu	859	19	O2	O2	NNP
cord-014597-66vd2mdu	859	20	,	,	,
cord-014597-66vd2mdu	859	21	metabolic	metabolic	JJ
cord-014597-66vd2mdu	859	22	data	datum	NNS
cord-014597-66vd2mdu	859	23	)	)	-RRB-
cord-014597-66vd2mdu	859	24	,	,	,
cord-014597-66vd2mdu	859	25	auto	auto	NN
cord-014597-66vd2mdu	859	26	-	-	HYPH
cord-014597-66vd2mdu	859	27	calculations	calculation	NNS
cord-014597-66vd2mdu	859	28	and	and	CC
cord-014597-66vd2mdu	859	29	aggregations	aggregation	NNS
cord-014597-66vd2mdu	859	30	(	(	-LRB-
cord-014597-66vd2mdu	859	31	e.g.	e.g.	RB
cord-014597-66vd2mdu	859	32	,	,	,
cord-014597-66vd2mdu	859	33	IVCD	IVCD	NNP
cord-014597-66vd2mdu	859	34	,	,	,
cord-014597-66vd2mdu	859	35	qP	qP	NNP
cord-014597-66vd2mdu	859	36	,	,	,
cord-014597-66vd2mdu	859	37	consumption	consumption	NN
cord-014597-66vd2mdu	859	38	rates	rate	NNS
cord-014597-66vd2mdu	859	39	)	)	-RRB-
cord-014597-66vd2mdu	859	40	and	and	CC
cord-014597-66vd2mdu	859	41	multi	multi	JJ
cord-014597-66vd2mdu	859	42	-	-	JJ
cord-014597-66vd2mdu	859	43	parametric	parametric	JJ
cord-014597-66vd2mdu	859	44	assessment	assessment	NN
cord-014597-66vd2mdu	859	45	of	of	IN
cord-014597-66vd2mdu	859	46	any	any	DT
cord-014597-66vd2mdu	859	47	type	type	NN
cord-014597-66vd2mdu	859	48	of	of	IN
cord-014597-66vd2mdu	859	49	time	time	NN
cord-014597-66vd2mdu	859	50	-	-	HYPH
cord-014597-66vd2mdu	859	51	series	series	NN
cord-014597-66vd2mdu	859	52	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	859	53	data	datum	NNS
cord-014597-66vd2mdu	859	54	in	in	IN
cord-014597-66vd2mdu	859	55	the	the	DT
cord-014597-66vd2mdu	859	56	context	context	NN
cord-014597-66vd2mdu	859	57	of	of	IN
cord-014597-66vd2mdu	859	58	experimental	experimental	JJ
cord-014597-66vd2mdu	859	59	protocol	protocol	NN
cord-014597-66vd2mdu	859	60	data	datum	NNS
cord-014597-66vd2mdu	859	61	(	(	-LRB-
cord-014597-66vd2mdu	859	62	e.g.	e.g.	RB
cord-014597-66vd2mdu	859	63	,	,	,
cord-014597-66vd2mdu	859	64	process	process	NN
cord-014597-66vd2mdu	859	65	parameters	parameter	NNS
cord-014597-66vd2mdu	859	66	,	,	,
cord-014597-66vd2mdu	859	67	feeds	feed	VBZ
cord-014597-66vd2mdu	859	68	)	)	-RRB-
cord-014597-66vd2mdu	859	69	.	.	.
cord-014597-66vd2mdu	860	1	Genedata	Genedata	NNP
cord-014597-66vd2mdu	860	2	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	860	3	comes	come	VBZ
cord-014597-66vd2mdu	860	4	with	with	IN
cord-014597-66vd2mdu	860	5	dedicated	dedicated	JJ
cord-014597-66vd2mdu	860	6	interfaces	interface	NNS
cord-014597-66vd2mdu	860	7	for	for	IN
cord-014597-66vd2mdu	860	8	integrating	integrate	VBG
cord-014597-66vd2mdu	860	9	with	with	IN
cord-014597-66vd2mdu	860	10	relevant	relevant	JJ
cord-014597-66vd2mdu	860	11	laboratory	laboratory	NN
cord-014597-66vd2mdu	860	12	instruments	instrument	NNS
cord-014597-66vd2mdu	860	13	,	,	,
cord-014597-66vd2mdu	860	14	control	control	NN
cord-014597-66vd2mdu	860	15	systems	system	NNS
cord-014597-66vd2mdu	860	16	,	,	,
cord-014597-66vd2mdu	860	17	statistical	statistical	JJ
cord-014597-66vd2mdu	860	18	analysis	analysis	NN
cord-014597-66vd2mdu	860	19	software	software	NN
cord-014597-66vd2mdu	860	20	packages	package	NNS
cord-014597-66vd2mdu	860	21	and	and	CC
cord-014597-66vd2mdu	860	22	custom	custom	NN
cord-014597-66vd2mdu	860	23	enterprise	enterprise	NN
cord-014597-66vd2mdu	860	24	solutions	solution	NNS
cord-014597-66vd2mdu	860	25	.	.	.
cord-014597-66vd2mdu	861	1	It	-PRON-	PRP
cord-014597-66vd2mdu	861	2	enables	enable	VBZ
cord-014597-66vd2mdu	861	3	the	the	DT
cord-014597-66vd2mdu	861	4	modeling	modeling	NN
cord-014597-66vd2mdu	861	5	and	and	CC
cord-014597-66vd2mdu	861	6	tracking	tracking	NN
cord-014597-66vd2mdu	861	7	of	of	IN
cord-014597-66vd2mdu	861	8	complex	complex	JJ
cord-014597-66vd2mdu	861	9	nonlinear	nonlinear	JJ
cord-014597-66vd2mdu	861	10	workflows	workflow	NNS
cord-014597-66vd2mdu	861	11	and	and	CC
cord-014597-66vd2mdu	861	12	supports	support	VBZ
cord-014597-66vd2mdu	861	13	decision	decision	NN
cord-014597-66vd2mdu	861	14	making	make	VBG
cord-014597-66vd2mdu	861	15	in	in	IN
cord-014597-66vd2mdu	861	16	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	861	17	development	development	NN
cord-014597-66vd2mdu	861	18	.	.	.
cord-014597-66vd2mdu	862	1	The	the	DT
cord-014597-66vd2mdu	862	2	data	datum	NNS
cord-014597-66vd2mdu	862	3	can	can	MD
cord-014597-66vd2mdu	862	4	be	be	VB
cord-014597-66vd2mdu	862	5	analyzed	analyze	VBN
cord-014597-66vd2mdu	862	6	in	in	IN
cord-014597-66vd2mdu	862	7	the	the	DT
cord-014597-66vd2mdu	862	8	context	context	NN
cord-014597-66vd2mdu	862	9	of	of	IN
cord-014597-66vd2mdu	862	10	upstream	upstream	JJ
cord-014597-66vd2mdu	862	11	process	process	NN
cord-014597-66vd2mdu	862	12	development	development	NN
cord-014597-66vd2mdu	862	13	,	,	,
cord-014597-66vd2mdu	862	14	and	and	CC
cord-014597-66vd2mdu	862	15	also	also	RB
cord-014597-66vd2mdu	862	16	be	be	VB
cord-014597-66vd2mdu	862	17	correlated	correlate	VBN
cord-014597-66vd2mdu	862	18	to	to	IN
cord-014597-66vd2mdu	862	19	other	other	JJ
cord-014597-66vd2mdu	862	20	unit	unit	NN
cord-014597-66vd2mdu	862	21	operations	operation	NNS
cord-014597-66vd2mdu	862	22	.	.	.
cord-014597-66vd2mdu	863	1	Automation	automation	NN
cord-014597-66vd2mdu	863	2	support	support	NN
cord-014597-66vd2mdu	863	3	assists	assist	VBZ
cord-014597-66vd2mdu	863	4	the	the	DT
cord-014597-66vd2mdu	863	5	ever	ever	RB
cord-014597-66vd2mdu	863	6	increasing	increase	VBG
cord-014597-66vd2mdu	863	7	throughput	throughput	NN
cord-014597-66vd2mdu	863	8	of	of	IN
cord-014597-66vd2mdu	863	9	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	863	10	development	development	NN
cord-014597-66vd2mdu	863	11	operations	operation	NNS
cord-014597-66vd2mdu	863	12	,	,	,
cord-014597-66vd2mdu	863	13	and	and	CC
cord-014597-66vd2mdu	863	14	the	the	DT
cord-014597-66vd2mdu	863	15	analysis	analysis	NN
cord-014597-66vd2mdu	863	16	of	of	IN
cord-014597-66vd2mdu	863	17	experimental	experimental	JJ
cord-014597-66vd2mdu	863	18	data	datum	NNS
cord-014597-66vd2mdu	863	19	and	and	CC
cord-014597-66vd2mdu	863	20	process	process	NN
cord-014597-66vd2mdu	863	21	parameters	parameter	NNS
cord-014597-66vd2mdu	863	22	across	across	IN
cord-014597-66vd2mdu	863	23	unit	unit	NN
cord-014597-66vd2mdu	863	24	operations	operation	NNS
cord-014597-66vd2mdu	863	25	or	or	CC
cord-014597-66vd2mdu	863	26	even	even	RB
cord-014597-66vd2mdu	863	27	different	different	JJ
cord-014597-66vd2mdu	863	28	projects	project	NNS
cord-014597-66vd2mdu	863	29	.	.	.
cord-014597-66vd2mdu	864	1	This	this	DT
cord-014597-66vd2mdu	864	2	overall	overall	JJ
cord-014597-66vd2mdu	864	3	integration	integration	NN
cord-014597-66vd2mdu	864	4	enhances	enhance	VBZ
cord-014597-66vd2mdu	864	5	process	process	NN
cord-014597-66vd2mdu	864	6	development	development	NN
cord-014597-66vd2mdu	864	7	workflows	workflow	NNS
cord-014597-66vd2mdu	864	8	.	.	.
cord-014597-66vd2mdu	865	1	Highlighted	highlight	VBN
cord-014597-66vd2mdu	865	2	use	use	NN
cord-014597-66vd2mdu	865	3	cases	case	NNS
cord-014597-66vd2mdu	865	4	describe	describe	VBP
cord-014597-66vd2mdu	865	5	the	the	DT
cord-014597-66vd2mdu	865	6	selection	selection	NN
cord-014597-66vd2mdu	865	7	of	of	IN
cord-014597-66vd2mdu	865	8	the	the	DT
cord-014597-66vd2mdu	865	9	best	good	JJS
cord-014597-66vd2mdu	865	10	producer	producer	NN
cord-014597-66vd2mdu	865	11	clones	clone	NNS
cord-014597-66vd2mdu	865	12	(	(	-LRB-
cord-014597-66vd2mdu	865	13	Fig	Fig	NNP
cord-014597-66vd2mdu	865	14	.	.	.
cord-014597-66vd2mdu	865	15	1a	1a	CD
cord-014597-66vd2mdu	865	16	)	)	-RRB-
cord-014597-66vd2mdu	865	17	,	,	,
cord-014597-66vd2mdu	865	18	the	the	DT
cord-014597-66vd2mdu	865	19	identification	identification	NN
cord-014597-66vd2mdu	865	20	of	of	IN
cord-014597-66vd2mdu	865	21	optimized	optimize	VBN
cord-014597-66vd2mdu	865	22	media	medium	NNS
cord-014597-66vd2mdu	865	23	feeding	feeding	NN
cord-014597-66vd2mdu	865	24	strategies	strategy	NNS
cord-014597-66vd2mdu	865	25	(	(	-LRB-
cord-014597-66vd2mdu	865	26	Fig	Fig	NNP
cord-014597-66vd2mdu	865	27	.	.	.
cord-014597-66vd2mdu	865	28	1b	1b	CD
cord-014597-66vd2mdu	865	29	)	)	-RRB-
cord-014597-66vd2mdu	865	30	,	,	,
cord-014597-66vd2mdu	865	31	and	and	CC
cord-014597-66vd2mdu	865	32	the	the	DT
cord-014597-66vd2mdu	865	33	comparison	comparison	NN
cord-014597-66vd2mdu	865	34	of	of	IN
cord-014597-66vd2mdu	865	35	clone	clone	NN
cord-014597-66vd2mdu	865	36	performance	performance	NN
cord-014597-66vd2mdu	865	37	across	across	IN
cord-014597-66vd2mdu	865	38	different	different	JJ
cord-014597-66vd2mdu	865	39	fermentation	fermentation	NN
cord-014597-66vd2mdu	865	40	scales	scale	NNS
cord-014597-66vd2mdu	865	41	(	(	-LRB-
cord-014597-66vd2mdu	865	42	Fig	Fig	NNP
cord-014597-66vd2mdu	865	43	.	.	.
cord-014597-66vd2mdu	865	44	1c	1c	CD
cord-014597-66vd2mdu	865	45	)	)	-RRB-
cord-014597-66vd2mdu	865	46	.	.	.
cord-014597-66vd2mdu	866	1	A	a	DT
cord-014597-66vd2mdu	866	2	special	special	JJ
cord-014597-66vd2mdu	866	3	focus	focus	NN
cord-014597-66vd2mdu	866	4	is	be	VBZ
cord-014597-66vd2mdu	866	5	on	on	IN
cord-014597-66vd2mdu	866	6	the	the	DT
cord-014597-66vd2mdu	866	7	analysis	analysis	NN
cord-014597-66vd2mdu	866	8	of	of	IN
cord-014597-66vd2mdu	866	9	data	datum	NNS
cord-014597-66vd2mdu	866	10	from	from	IN
cord-014597-66vd2mdu	866	11	micro	micro	JJ
cord-014597-66vd2mdu	866	12	-	-	HYPH
cord-014597-66vd2mdu	866	13	and	and	CC
cord-014597-66vd2mdu	866	14	bench	bench	NN
cord-014597-66vd2mdu	866	15	-	-	HYPH
cord-014597-66vd2mdu	866	16	top	top	NN
cord-014597-66vd2mdu	866	17	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	866	18	(	(	-LRB-
cord-014597-66vd2mdu	866	19	such	such	JJ
cord-014597-66vd2mdu	866	20	as	as	IN
cord-014597-66vd2mdu	866	21	the	the	DT
cord-014597-66vd2mdu	866	22	ambr15	ambr15	NN
cord-014597-66vd2mdu	866	23	™	™	NN
cord-014597-66vd2mdu	866	24	and	and	CC
cord-014597-66vd2mdu	866	25	DasGip	DasGip	NNP
cord-014597-66vd2mdu	866	26	™	™	NNP
cord-014597-66vd2mdu	866	27	systems	system	NNS
cord-014597-66vd2mdu	866	28	)	)	-RRB-
cord-014597-66vd2mdu	866	29	operated	operate	VBD
cord-014597-66vd2mdu	866	30	in	in	IN
cord-014597-66vd2mdu	866	31	parallel	parallel	NN
cord-014597-66vd2mdu	866	32	.	.	.
cord-014597-66vd2mdu	867	1	These	these	DT
cord-014597-66vd2mdu	867	2	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	867	3	allow	allow	VBP
cord-014597-66vd2mdu	867	4	for	for	IN
cord-014597-66vd2mdu	867	5	increased	increase	VBN
cord-014597-66vd2mdu	867	6	throughput	throughput	NN
cord-014597-66vd2mdu	867	7	of	of	IN
cord-014597-66vd2mdu	867	8	clone	clone	NN
cord-014597-66vd2mdu	867	9	selection	selection	NN
cord-014597-66vd2mdu	867	10	and	and	CC
cord-014597-66vd2mdu	867	11	process	process	NN
cord-014597-66vd2mdu	867	12	optimization	optimization	NN
cord-014597-66vd2mdu	867	13	studies	study	NNS
cord-014597-66vd2mdu	867	14	,	,	,
cord-014597-66vd2mdu	867	15	which	which	WDT
cord-014597-66vd2mdu	867	16	in	in	IN
cord-014597-66vd2mdu	867	17	turn	turn	NN
cord-014597-66vd2mdu	867	18	leads	lead	VBZ
cord-014597-66vd2mdu	867	19	to	to	IN
cord-014597-66vd2mdu	867	20	an	an	DT
cord-014597-66vd2mdu	867	21	increase	increase	NN
cord-014597-66vd2mdu	867	22	in	in	IN
cord-014597-66vd2mdu	867	23	data	datum	NNS
cord-014597-66vd2mdu	867	24	generation	generation	NN
cord-014597-66vd2mdu	867	25	.	.	.
cord-014597-66vd2mdu	868	1	Genedata	Genedata	NNP
cord-014597-66vd2mdu	868	2	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	868	3	supports	support	VBZ
cord-014597-66vd2mdu	868	4	integration	integration	NN
cord-014597-66vd2mdu	868	5	with	with	IN
cord-014597-66vd2mdu	868	6	such	such	JJ
cord-014597-66vd2mdu	868	7	systems	system	NNS
cord-014597-66vd2mdu	868	8	and	and	CC
cord-014597-66vd2mdu	868	9	enables	enable	VBZ
cord-014597-66vd2mdu	868	10	a	a	DT
cord-014597-66vd2mdu	868	11	comparison	comparison	NN
cord-014597-66vd2mdu	868	12	of	of	IN
cord-014597-66vd2mdu	868	13	data	datum	NNS
cord-014597-66vd2mdu	868	14	regardless	regardless	RB
cord-014597-66vd2mdu	868	15	of	of	IN
cord-014597-66vd2mdu	868	16	the	the	DT
cord-014597-66vd2mdu	868	17	instrument	instrument	NN
cord-014597-66vd2mdu	868	18	provider	provider	NN
cord-014597-66vd2mdu	868	19	or	or	CC
cord-014597-66vd2mdu	868	20	scale	scale	NN
cord-014597-66vd2mdu	868	21	.	.	.
cord-014597-66vd2mdu	869	1	Automated	automate	VBN
cord-014597-66vd2mdu	869	2	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	869	3	data	datum	NNS
cord-014597-66vd2mdu	869	4	analysis	analysis	NN
cord-014597-66vd2mdu	869	5	allows	allow	VBZ
cord-014597-66vd2mdu	869	6	development	development	NN
cord-014597-66vd2mdu	869	7	groups	group	NNS
cord-014597-66vd2mdu	869	8	to	to	TO
cord-014597-66vd2mdu	869	9	take	take	VB
cord-014597-66vd2mdu	869	10	advantage	advantage	NN
cord-014597-66vd2mdu	869	11	of	of	IN
cord-014597-66vd2mdu	869	12	even	even	RB
cord-014597-66vd2mdu	869	13	richer	rich	JJR
cord-014597-66vd2mdu	869	14	datasets	dataset	NNS
cord-014597-66vd2mdu	869	15	and	and	CC
cord-014597-66vd2mdu	869	16	,	,	,
cord-014597-66vd2mdu	869	17	as	as	IN
cord-014597-66vd2mdu	869	18	data	data	NN
cord-014597-66vd2mdu	869	19	management	management	NN
cord-014597-66vd2mdu	869	20	is	be	VBZ
cord-014597-66vd2mdu	869	21	built	build	VBN
cord-014597-66vd2mdu	869	22	-	-	HYPH
cord-014597-66vd2mdu	869	23	in	in	RP
cord-014597-66vd2mdu	869	24	to	to	IN
cord-014597-66vd2mdu	869	25	the	the	DT
cord-014597-66vd2mdu	869	26	system	system	NN
cord-014597-66vd2mdu	869	27	,	,	,
cord-014597-66vd2mdu	869	28	the	the	DT
cord-014597-66vd2mdu	869	29	data	datum	NNS
cord-014597-66vd2mdu	869	30	can	can	MD
cord-014597-66vd2mdu	869	31	be	be	VB
cord-014597-66vd2mdu	869	32	easily	easily	RB
cord-014597-66vd2mdu	869	33	tracked	track	VBN
cord-014597-66vd2mdu	869	34	and	and	CC
cord-014597-66vd2mdu	869	35	associated	associate	VBN
cord-014597-66vd2mdu	869	36	to	to	IN
cord-014597-66vd2mdu	869	37	historical	historical	JJ
cord-014597-66vd2mdu	869	38	records	record	NNS
cord-014597-66vd2mdu	869	39	.	.	.
cord-014597-66vd2mdu	870	1	Another	another	DT
cord-014597-66vd2mdu	870	2	focus	focus	NN
cord-014597-66vd2mdu	870	3	is	be	VBZ
cord-014597-66vd2mdu	870	4	on	on	IN
cord-014597-66vd2mdu	870	5	cross	cross	JJ
cord-014597-66vd2mdu	870	6	-	-	JJ
cord-014597-66vd2mdu	870	7	reactor	reactor	JJ
cord-014597-66vd2mdu	870	8	scale	scale	NN
cord-014597-66vd2mdu	870	9	comparisons	comparison	NNS
cord-014597-66vd2mdu	870	10	.	.	.
cord-014597-66vd2mdu	871	1	Data	datum	NNS
cord-014597-66vd2mdu	871	2	coming	come	VBG
cord-014597-66vd2mdu	871	3	from	from	IN
cord-014597-66vd2mdu	871	4	different	different	JJ
cord-014597-66vd2mdu	871	5	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	871	6	scales	scale	NNS
cord-014597-66vd2mdu	871	7	can	can	MD
cord-014597-66vd2mdu	871	8	be	be	VB
cord-014597-66vd2mdu	871	9	easily	easily	RB
cord-014597-66vd2mdu	871	10	imported	import	VBN
cord-014597-66vd2mdu	871	11	into	into	IN
cord-014597-66vd2mdu	871	12	the	the	DT
cord-014597-66vd2mdu	871	13	platform	platform	NN
cord-014597-66vd2mdu	871	14	and	and	CC
cord-014597-66vd2mdu	871	15	analyzed	analyze	VBD
cord-014597-66vd2mdu	871	16	to	to	TO
cord-014597-66vd2mdu	871	17	establish	establish	VB
cord-014597-66vd2mdu	871	18	the	the	DT
cord-014597-66vd2mdu	871	19	best	good	JJS
cord-014597-66vd2mdu	871	20	conditions	condition	NNS
cord-014597-66vd2mdu	871	21	for	for	IN
cord-014597-66vd2mdu	871	22	upscaling	upscaling	NN
cord-014597-66vd2mdu	871	23	.	.	.
cord-014597-66vd2mdu	872	1	Genedata	Genedata	NNP
cord-014597-66vd2mdu	872	2	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	872	3	enables	enable	VBZ
cord-014597-66vd2mdu	872	4	the	the	DT
cord-014597-66vd2mdu	872	5	correlation	correlation	NN
cord-014597-66vd2mdu	872	6	of	of	IN
cord-014597-66vd2mdu	872	7	process	process	NN
cord-014597-66vd2mdu	872	8	parameters	parameter	NNS
cord-014597-66vd2mdu	872	9	(	(	-LRB-
cord-014597-66vd2mdu	872	10	e.g.	e.g.	RB
cord-014597-66vd2mdu	872	11	,	,	,
cord-014597-66vd2mdu	872	12	fermentation	fermentation	NN
cord-014597-66vd2mdu	872	13	protocols	protocol	NNS
cord-014597-66vd2mdu	872	14	,	,	,
cord-014597-66vd2mdu	872	15	media	medium	NNS
cord-014597-66vd2mdu	872	16	recipes	recipe	NNS
cord-014597-66vd2mdu	872	17	,	,	,
cord-014597-66vd2mdu	872	18	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	872	19	control	control	NN
cord-014597-66vd2mdu	872	20	parameters	parameter	NNS
cord-014597-66vd2mdu	872	21	)	)	-RRB-
cord-014597-66vd2mdu	872	22	,	,	,
cord-014597-66vd2mdu	872	23	with	with	IN
cord-014597-66vd2mdu	872	24	key	key	JJ
cord-014597-66vd2mdu	872	25	performance	performance	NN
cord-014597-66vd2mdu	872	26	indicators	indicator	NNS
cord-014597-66vd2mdu	872	27	of	of	IN
cord-014597-66vd2mdu	872	28	the	the	DT
cord-014597-66vd2mdu	872	29	processes	process	NNS
cord-014597-66vd2mdu	872	30	(	(	-LRB-
cord-014597-66vd2mdu	872	31	e.g.	e.g.	RB
cord-014597-66vd2mdu	872	32	,	,	,
cord-014597-66vd2mdu	872	33	Titer	Titer	NNP
cord-014597-66vd2mdu	872	34	,	,	,
cord-014597-66vd2mdu	872	35	qP	qP	NNP
cord-014597-66vd2mdu	872	36	)	)	-RRB-
cord-014597-66vd2mdu	872	37	and	and	CC
cord-014597-66vd2mdu	872	38	the	the	DT
cord-014597-66vd2mdu	872	39	product	product	NN
cord-014597-66vd2mdu	872	40	quality	quality	NN
cord-014597-66vd2mdu	872	41	attributes	attribute	NNS
cord-014597-66vd2mdu	872	42	(	(	-LRB-
cord-014597-66vd2mdu	872	43	e.g.	e.g.	RB
cord-014597-66vd2mdu	872	44	,	,	,
cord-014597-66vd2mdu	872	45	aggregation	aggregation	NN
cord-014597-66vd2mdu	872	46	,	,	,
cord-014597-66vd2mdu	872	47	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	872	48	profiles	profile	NNS
cord-014597-66vd2mdu	872	49	)	)	-RRB-
cord-014597-66vd2mdu	872	50	.	.	.
cord-014597-66vd2mdu	873	1	Finally	finally	RB
cord-014597-66vd2mdu	873	2	,	,	,
cord-014597-66vd2mdu	873	3	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	873	4	time	time	NN
cord-014597-66vd2mdu	873	5	course	course	NN
cord-014597-66vd2mdu	873	6	data	datum	NNS
cord-014597-66vd2mdu	873	7	can	can	MD
cord-014597-66vd2mdu	873	8	be	be	VB
cord-014597-66vd2mdu	873	9	tracked	track	VBN
cord-014597-66vd2mdu	873	10	together	together	RB
cord-014597-66vd2mdu	873	11	with	with	IN
cord-014597-66vd2mdu	873	12	clone	clone	NN
cord-014597-66vd2mdu	873	13	analytics	analytic	NNS
cord-014597-66vd2mdu	873	14	and	and	CC
cord-014597-66vd2mdu	873	15	product	product	NN
cord-014597-66vd2mdu	873	16	quality	quality	NN
cord-014597-66vd2mdu	873	17	parameters	parameter	NNS
cord-014597-66vd2mdu	873	18	,	,	,
cord-014597-66vd2mdu	873	19	which	which	WDT
cord-014597-66vd2mdu	873	20	makes	make	VBZ
cord-014597-66vd2mdu	873	21	the	the	DT
cord-014597-66vd2mdu	873	22	platform	platform	NN
cord-014597-66vd2mdu	873	23	uniquely	uniquely	RB
cord-014597-66vd2mdu	873	24	able	able	JJ
cord-014597-66vd2mdu	873	25	to	to	TO
cord-014597-66vd2mdu	873	26	support	support	VB
cord-014597-66vd2mdu	873	27	end	end	NN
cord-014597-66vd2mdu	873	28	-	-	HYPH
cord-014597-66vd2mdu	873	29	to	to	IN
cord-014597-66vd2mdu	873	30	-	-	HYPH
cord-014597-66vd2mdu	873	31	end	end	NN
cord-014597-66vd2mdu	873	32	biopharma	biopharma	NN
cord-014597-66vd2mdu	873	33	development	development	NN
cord-014597-66vd2mdu	873	34	.	.	.
cord-014597-66vd2mdu	874	1	Upstream	upstream	JJ
cord-014597-66vd2mdu	874	2	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	874	3	are	be	VBP
cord-014597-66vd2mdu	874	4	at	at	IN
cord-014597-66vd2mdu	874	5	particular	particular	JJ
cord-014597-66vd2mdu	874	6	risk	risk	NN
cord-014597-66vd2mdu	874	7	of	of	IN
cord-014597-66vd2mdu	874	8	contamination	contamination	NN
cord-014597-66vd2mdu	874	9	from	from	IN
cord-014597-66vd2mdu	874	10	adventitious	adventitious	JJ
cord-014597-66vd2mdu	874	11	agents	agent	NNS
cord-014597-66vd2mdu	874	12	.	.	.
cord-014597-66vd2mdu	875	1	The	the	DT
cord-014597-66vd2mdu	875	2	typical	typical	JJ
cord-014597-66vd2mdu	875	3	0.1	0.1	CD
cord-014597-66vd2mdu	875	4	μm	μm	NN
cord-014597-66vd2mdu	875	5	filters	filter	NNS
cord-014597-66vd2mdu	875	6	used	use	VBN
cord-014597-66vd2mdu	875	7	at	at	IN
cord-014597-66vd2mdu	875	8	this	this	DT
cord-014597-66vd2mdu	875	9	step	step	NN
cord-014597-66vd2mdu	875	10	protect	protect	VBP
cord-014597-66vd2mdu	875	11	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	875	12	from	from	IN
cord-014597-66vd2mdu	875	13	bacteria	bacteria	NNS
cord-014597-66vd2mdu	875	14	and	and	CC
cord-014597-66vd2mdu	875	15	mycoplasma	mycoplasma	NN
cord-014597-66vd2mdu	875	16	but	but	CC
cord-014597-66vd2mdu	875	17	offer	offer	VBP
cord-014597-66vd2mdu	875	18	no	no	DT
cord-014597-66vd2mdu	875	19	protection	protection	NN
cord-014597-66vd2mdu	875	20	from	from	IN
cord-014597-66vd2mdu	875	21	viral	viral	JJ
cord-014597-66vd2mdu	875	22	contaminations	contamination	NNS
cord-014597-66vd2mdu	875	23	.	.	.
cord-014597-66vd2mdu	876	1	A	a	DT
cord-014597-66vd2mdu	876	2	new	new	JJ
cord-014597-66vd2mdu	876	3	polyethersulfone	polyethersulfone	NNP
cord-014597-66vd2mdu	876	4	(	(	-LRB-
cord-014597-66vd2mdu	876	5	PES	PES	NNP
cord-014597-66vd2mdu	876	6	)	)	-RRB-
cord-014597-66vd2mdu	876	7	upstream	upstream	JJ
cord-014597-66vd2mdu	876	8	virus	virus	NN
cord-014597-66vd2mdu	876	9	filter	filter	NN
cord-014597-66vd2mdu	876	10	,	,	,
cord-014597-66vd2mdu	876	11	Viresolve	Viresolve	NNP
cord-014597-66vd2mdu	876	12	®	®	NNP
cord-014597-66vd2mdu	876	13	Barrier	Barrier	NNP
cord-014597-66vd2mdu	876	14	,	,	,
cord-014597-66vd2mdu	876	15	has	have	VBZ
cord-014597-66vd2mdu	876	16	demonstrated	demonstrate	VBN
cord-014597-66vd2mdu	876	17	high	high	JJ
cord-014597-66vd2mdu	876	18	levels	level	NNS
cord-014597-66vd2mdu	876	19	of	of	IN
cord-014597-66vd2mdu	877	1	microorganism	microorganism	NNP
cord-014597-66vd2mdu	877	2	retention	retention	NN
cord-014597-66vd2mdu	877	3	-full	-full	VBZ
cord-014597-66vd2mdu	877	4	retention	retention	NN
cord-014597-66vd2mdu	877	5	for	for	IN
cord-014597-66vd2mdu	877	6	bacteria	bacteria	NNS
cord-014597-66vd2mdu	877	7	and	and	CC
cord-014597-66vd2mdu	877	8	mycoplasma	mycoplasma	NN
cord-014597-66vd2mdu	877	9	(	(	-LRB-
cord-014597-66vd2mdu	877	10	>	>	NFP
cord-014597-66vd2mdu	877	11	8.0	8.0	CD
cord-014597-66vd2mdu	877	12	LRV	lrv	NN
cord-014597-66vd2mdu	877	13	-Log	-Log	HYPH
cord-014597-66vd2mdu	877	14	Reduction	Reduction	NNP
cord-014597-66vd2mdu	877	15	Value	Value	NNP
cord-014597-66vd2mdu	877	16	)	)	-RRB-
cord-014597-66vd2mdu	877	17	and~5	and~5	JJ
cord-014597-66vd2mdu	877	18	LRV	lrv	NN
cord-014597-66vd2mdu	877	19	for	for	IN
cord-014597-66vd2mdu	877	20	small	small	JJ
cord-014597-66vd2mdu	877	21	viruses	virus	NNS
cord-014597-66vd2mdu	877	22	,	,	,
cord-014597-66vd2mdu	877	23	such	such	JJ
cord-014597-66vd2mdu	877	24	as	as	IN
cord-014597-66vd2mdu	877	25	parvoviruses	parvovirus	NNS
cord-014597-66vd2mdu	877	26	.	.	.
cord-014597-66vd2mdu	878	1	It	-PRON-	PRP
cord-014597-66vd2mdu	878	2	also	also	RB
cord-014597-66vd2mdu	878	3	has	have	VBZ
cord-014597-66vd2mdu	878	4	improved	improve	VBN
cord-014597-66vd2mdu	878	5	flow	flow	NN
cord-014597-66vd2mdu	878	6	and	and	CC
cord-014597-66vd2mdu	878	7	capacity	capacity	NN
cord-014597-66vd2mdu	878	8	as	as	IN
cord-014597-66vd2mdu	878	9	compared	compare	VBN
cord-014597-66vd2mdu	878	10	to	to	IN
cord-014597-66vd2mdu	878	11	virus	virus	NN
cord-014597-66vd2mdu	878	12	removal	removal	NN
cord-014597-66vd2mdu	878	13	filters	filter	NNS
cord-014597-66vd2mdu	878	14	designed	design	VBN
cord-014597-66vd2mdu	878	15	for	for	IN
cord-014597-66vd2mdu	878	16	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	878	17	antibody	antibody	NN
cord-014597-66vd2mdu	878	18	purification	purification	NN
cord-014597-66vd2mdu	878	19	.	.	.
cord-014597-66vd2mdu	879	1	Given	give	VBN
cord-014597-66vd2mdu	879	2	the	the	DT
cord-014597-66vd2mdu	879	3	small	small	JJ
cord-014597-66vd2mdu	879	4	pore	pore	NN
cord-014597-66vd2mdu	879	5	size	size	NN
cord-014597-66vd2mdu	879	6	of	of	IN
cord-014597-66vd2mdu	879	7	virus	virus	NN
cord-014597-66vd2mdu	879	8	retentive	retentive	JJ
cord-014597-66vd2mdu	879	9	filters	filter	NNS
cord-014597-66vd2mdu	879	10	,	,	,
cord-014597-66vd2mdu	879	11	implementing	implement	VBG
cord-014597-66vd2mdu	879	12	a	a	DT
cord-014597-66vd2mdu	879	13	virus	virus	NN
cord-014597-66vd2mdu	879	14	filter	filter	NN
cord-014597-66vd2mdu	879	15	upstream	upstream	RB
cord-014597-66vd2mdu	879	16	of	of	IN
cord-014597-66vd2mdu	879	17	the	the	DT
cord-014597-66vd2mdu	879	18	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	879	19	raises	raise	VBZ
cord-014597-66vd2mdu	879	20	the	the	DT
cord-014597-66vd2mdu	879	21	question	question	NN
cord-014597-66vd2mdu	879	22	of	of	IN
cord-014597-66vd2mdu	879	23	whether	whether	IN
cord-014597-66vd2mdu	879	24	critical	critical	JJ
cord-014597-66vd2mdu	879	25	cell	cell	NN
cord-014597-66vd2mdu	879	26	culture	culture	NN
cord-014597-66vd2mdu	879	27	media	medium	NNS
cord-014597-66vd2mdu	879	28	components	component	NNS
cord-014597-66vd2mdu	879	29	are	be	VBP
cord-014597-66vd2mdu	879	30	removed	remove	VBN
cord-014597-66vd2mdu	879	31	.	.	.
cord-014597-66vd2mdu	880	1	Therefore	therefore	RB
cord-014597-66vd2mdu	880	2	,	,	,
cord-014597-66vd2mdu	880	3	it	-PRON-	PRP
cord-014597-66vd2mdu	880	4	is	be	VBZ
cord-014597-66vd2mdu	880	5	important	important	JJ
cord-014597-66vd2mdu	880	6	to	to	TO
cord-014597-66vd2mdu	880	7	evaluate	evaluate	VB
cord-014597-66vd2mdu	880	8	the	the	DT
cord-014597-66vd2mdu	880	9	cell	cell	NN
cord-014597-66vd2mdu	880	10	culture	culture	NN
cord-014597-66vd2mdu	880	11	performance	performance	NN
cord-014597-66vd2mdu	880	12	and	and	CC
cord-014597-66vd2mdu	880	13	protein	protein	NN
cord-014597-66vd2mdu	880	14	quality	quality	NN
cord-014597-66vd2mdu	880	15	attributes	attribute	NNS
cord-014597-66vd2mdu	880	16	using	use	VBG
cord-014597-66vd2mdu	880	17	virus	virus	NN
cord-014597-66vd2mdu	880	18	-	-	HYPH
cord-014597-66vd2mdu	880	19	filtered	filter	VBN
cord-014597-66vd2mdu	880	20	media	medium	NNS
cord-014597-66vd2mdu	880	21	to	to	TO
cord-014597-66vd2mdu	880	22	ensure	ensure	VB
cord-014597-66vd2mdu	880	23	that	that	IN
cord-014597-66vd2mdu	880	24	filtration	filtration	NN
cord-014597-66vd2mdu	880	25	does	do	VBZ
cord-014597-66vd2mdu	880	26	not	not	RB
cord-014597-66vd2mdu	880	27	negatively	negatively	RB
cord-014597-66vd2mdu	880	28	impact	impact	VB
cord-014597-66vd2mdu	880	29	the	the	DT
cord-014597-66vd2mdu	880	30	process	process	NN
cord-014597-66vd2mdu	880	31	.	.	.
cord-014597-66vd2mdu	881	1	Materials	material	NNS
cord-014597-66vd2mdu	881	2	and	and	CC
cord-014597-66vd2mdu	881	3	methods	method	NNS
cord-014597-66vd2mdu	881	4	EX	ex	NN
cord-014597-66vd2mdu	881	5	-	-	HYPH
cord-014597-66vd2mdu	881	6	CELL	cell	NN
cord-014597-66vd2mdu	882	1	®	®	,
cord-014597-66vd2mdu	882	2	CHO	CHO	NNP
cord-014597-66vd2mdu	882	3	media	medium	NNS
cord-014597-66vd2mdu	882	4	and	and	CC
cord-014597-66vd2mdu	882	5	corresponding	corresponding	JJ
cord-014597-66vd2mdu	882	6	feeds	feed	NNS
cord-014597-66vd2mdu	882	7	were	be	VBD
cord-014597-66vd2mdu	882	8	processed	process	VBN
cord-014597-66vd2mdu	882	9	through	through	IN
cord-014597-66vd2mdu	882	10	either	either	DT
cord-014597-66vd2mdu	882	11	Viresolve	Viresolve	NNP
cord-014597-66vd2mdu	882	12	®	®	.
cord-014597-66vd2mdu	882	13	Barrier	Barrier	NNP
cord-014597-66vd2mdu	882	14	filters	filter	NNS
cord-014597-66vd2mdu	882	15	or	or	CC
cord-014597-66vd2mdu	882	16	0.22	0.22	CD
cord-014597-66vd2mdu	882	17	μm	μm	NN
cord-014597-66vd2mdu	882	18	filters	filter	NNS
cord-014597-66vd2mdu	882	19	(	(	-LRB-
cord-014597-66vd2mdu	882	20	control	control	NN
cord-014597-66vd2mdu	882	21	)	)	-RRB-
cord-014597-66vd2mdu	882	22	.	.	.
cord-014597-66vd2mdu	883	1	Media	medium	NNS
cord-014597-66vd2mdu	883	2	composition	composition	NN
cord-014597-66vd2mdu	883	3	post	post	NN
cord-014597-66vd2mdu	883	4	-	-	NN
cord-014597-66vd2mdu	883	5	filtration	filtration	NN
cord-014597-66vd2mdu	883	6	was	be	VBD
cord-014597-66vd2mdu	883	7	evaluated	evaluate	VBN
cord-014597-66vd2mdu	883	8	by	by	IN
cord-014597-66vd2mdu	883	9	high	high	JJ
cord-014597-66vd2mdu	883	10	performance	performance	NN
cord-014597-66vd2mdu	883	11	liquid	liquid	NN
cord-014597-66vd2mdu	883	12	chromatography	chromatography	NN
cord-014597-66vd2mdu	883	13	(	(	-LRB-
cord-014597-66vd2mdu	883	14	HPLC	HPLC	NNP
cord-014597-66vd2mdu	883	15	)	)	-RRB-
cord-014597-66vd2mdu	883	16	,	,	,
cord-014597-66vd2mdu	883	17	inductively	inductively	RB
cord-014597-66vd2mdu	883	18	coupled	couple	VBN
cord-014597-66vd2mdu	883	19	plasma/	plasma/	VBG
cord-014597-66vd2mdu	883	20	optical	optical	JJ
cord-014597-66vd2mdu	883	21	emission	emission	NN
cord-014597-66vd2mdu	883	22	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	883	23	(	(	-LRB-
cord-014597-66vd2mdu	883	24	ICP	ICP	NNP
cord-014597-66vd2mdu	883	25	-	-	HYPH
cord-014597-66vd2mdu	883	26	OES	OES	NNP
cord-014597-66vd2mdu	883	27	)	)	-RRB-
cord-014597-66vd2mdu	883	28	,	,	,
cord-014597-66vd2mdu	883	29	and	and	CC
cord-014597-66vd2mdu	883	30	nuclear	nuclear	JJ
cord-014597-66vd2mdu	883	31	magnetic	magnetic	JJ
cord-014597-66vd2mdu	883	32	resonance	resonance	NN
cord-014597-66vd2mdu	883	33	(	(	-LRB-
cord-014597-66vd2mdu	883	34	NMR	NMR	NNP
cord-014597-66vd2mdu	883	35	)	)	-RRB-
cord-014597-66vd2mdu	883	36	.	.	.
cord-014597-66vd2mdu	884	1	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	884	2	CHO	CHO	NNP
cord-014597-66vd2mdu	884	3	cells	cell	NNS
cord-014597-66vd2mdu	884	4	were	be	VBD
cord-014597-66vd2mdu	884	5	cultured	culture	VBN
cord-014597-66vd2mdu	884	6	in	in	IN
cord-014597-66vd2mdu	884	7	fed	fed	NNP
cord-014597-66vd2mdu	884	8	batch	batch	NN
cord-014597-66vd2mdu	884	9	culture	culture	NN
cord-014597-66vd2mdu	884	10	.	.	.
cord-014597-66vd2mdu	885	1	Cell	cell	NN
cord-014597-66vd2mdu	885	2	density	density	NN
cord-014597-66vd2mdu	885	3	and	and	CC
cord-014597-66vd2mdu	885	4	viability	viability	NN
cord-014597-66vd2mdu	885	5	were	be	VBD
cord-014597-66vd2mdu	885	6	measured	measure	VBN
cord-014597-66vd2mdu	885	7	by	by	IN
cord-014597-66vd2mdu	885	8	Vi	Vi	NNP
cord-014597-66vd2mdu	885	9	-	-	HYPH
cord-014597-66vd2mdu	885	10	Cell	Cell	NNP
cord-014597-66vd2mdu	885	11	TM	TM	NNP
cord-014597-66vd2mdu	885	12	cell	cell	NN
cord-014597-66vd2mdu	885	13	viability	viability	NN
cord-014597-66vd2mdu	885	14	analyzer	analyzer	NN
cord-014597-66vd2mdu	885	15	while	while	IN
cord-014597-66vd2mdu	885	16	metabolites	metabolite	NNS
cord-014597-66vd2mdu	885	17	were	be	VBD
cord-014597-66vd2mdu	885	18	analyzed	analyze	VBN
cord-014597-66vd2mdu	885	19	by	by	IN
cord-014597-66vd2mdu	885	20	BioProfile	BioProfile	NNP
cord-014597-66vd2mdu	885	21	®	®	SYM
cord-014597-66vd2mdu	885	22	FLEX	flex	NN
cord-014597-66vd2mdu	885	23	analyzer	analyzer	NN
cord-014597-66vd2mdu	885	24	.	.	.
cord-014597-66vd2mdu	886	1	Shake	shake	VB
cord-014597-66vd2mdu	886	2	flasks	flask	NNS
cord-014597-66vd2mdu	886	3	and	and	CC
cord-014597-66vd2mdu	886	4	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	886	5	were	be	VBD
cord-014597-66vd2mdu	886	6	utilized	utilize	VBN
cord-014597-66vd2mdu	886	7	to	to	TO
cord-014597-66vd2mdu	886	8	verify	verify	VB
cord-014597-66vd2mdu	886	9	that	that	IN
cord-014597-66vd2mdu	886	10	surfactants	surfactant	NNS
cord-014597-66vd2mdu	886	11	,	,	,
cord-014597-66vd2mdu	886	12	such	such	JJ
cord-014597-66vd2mdu	886	13	as	as	IN
cord-014597-66vd2mdu	886	14	poloxamer	poloxamer	NNP
cord-014597-66vd2mdu	886	15	,	,	,
cord-014597-66vd2mdu	886	16	(	(	-LRB-
cord-014597-66vd2mdu	886	17	which	which	WDT
cord-014597-66vd2mdu	886	18	are	be	VBP
cord-014597-66vd2mdu	886	19	essential	essential	JJ
cord-014597-66vd2mdu	886	20	for	for	IN
cord-014597-66vd2mdu	886	21	shear	shear	NN
cord-014597-66vd2mdu	886	22	protection	protection	NN
cord-014597-66vd2mdu	886	23	in	in	IN
cord-014597-66vd2mdu	886	24	stirred	stir	VBN
cord-014597-66vd2mdu	886	25	tank	tank	NN
cord-014597-66vd2mdu	886	26	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	886	27	and	and	CC
cord-014597-66vd2mdu	886	28	can	can	MD
cord-014597-66vd2mdu	886	29	be	be	VB
cord-014597-66vd2mdu	886	30	difficult	difficult	JJ
cord-014597-66vd2mdu	886	31	to	to	TO
cord-014597-66vd2mdu	886	32	filter	filter	VB
cord-014597-66vd2mdu	886	33	)	)	-RRB-
cord-014597-66vd2mdu	886	34	have	have	VBP
cord-014597-66vd2mdu	886	35	not	not	RB
cord-014597-66vd2mdu	886	36	been	be	VBN
cord-014597-66vd2mdu	886	37	removed	remove	VBN
cord-014597-66vd2mdu	886	38	during	during	IN
cord-014597-66vd2mdu	886	39	filtration	filtration	NN
cord-014597-66vd2mdu	886	40	.	.	.
cord-014597-66vd2mdu	887	1	Monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	887	2	antibody	antibody	NN
cord-014597-66vd2mdu	887	3	titer	titer	NN
cord-014597-66vd2mdu	887	4	was	be	VBD
cord-014597-66vd2mdu	887	5	quantitated	quantitate	VBN
cord-014597-66vd2mdu	887	6	by	by	IN
cord-014597-66vd2mdu	887	7	Protein	Protein	NNP
cord-014597-66vd2mdu	887	8	A	a	DT
cord-014597-66vd2mdu	887	9	HPLC	HPLC	NNP
cord-014597-66vd2mdu	887	10	.	.	.
cord-014597-66vd2mdu	888	1	Characterization	characterization	NN
cord-014597-66vd2mdu	888	2	of	of	IN
cord-014597-66vd2mdu	888	3	the	the	DT
cord-014597-66vd2mdu	888	4	antibody	antibody	NN
cord-014597-66vd2mdu	888	5	product	product	NN
cord-014597-66vd2mdu	888	6	quality	quality	NN
cord-014597-66vd2mdu	888	7	was	be	VBD
cord-014597-66vd2mdu	888	8	assessed	assess	VBN
cord-014597-66vd2mdu	888	9	via	via	IN
cord-014597-66vd2mdu	888	10	weak	weak	JJ
cord-014597-66vd2mdu	888	11	cation	cation	NN
cord-014597-66vd2mdu	888	12	-	-	HYPH
cord-014597-66vd2mdu	888	13	exchange	exchange	NN
cord-014597-66vd2mdu	888	14	chromatography	chromatography	NN
cord-014597-66vd2mdu	888	15	(	(	-LRB-
cord-014597-66vd2mdu	888	16	charge	charge	NN
cord-014597-66vd2mdu	888	17	heterogeneity	heterogeneity	NN
cord-014597-66vd2mdu	888	18	)	)	-RRB-
cord-014597-66vd2mdu	888	19	,	,	,
cord-014597-66vd2mdu	888	20	size	size	NN
cord-014597-66vd2mdu	888	21	exclusion	exclusion	NN
cord-014597-66vd2mdu	888	22	chromatography	chromatography	NN
cord-014597-66vd2mdu	888	23	(	(	-LRB-
cord-014597-66vd2mdu	888	24	aggregate	aggregate	NN
cord-014597-66vd2mdu	888	25	profile	profile	NN
cord-014597-66vd2mdu	888	26	)	)	-RRB-
cord-014597-66vd2mdu	888	27	,	,	,
cord-014597-66vd2mdu	888	28	and	and	CC
cord-014597-66vd2mdu	888	29	2-AB	2-ab	CD
cord-014597-66vd2mdu	888	30	fluorescent	fluorescent	JJ
cord-014597-66vd2mdu	888	31	labeling	labeling	NN
cord-014597-66vd2mdu	888	32	with	with	IN
cord-014597-66vd2mdu	888	33	NP	NP	NNP
cord-014597-66vd2mdu	888	34	-	-	HYPH
cord-014597-66vd2mdu	888	35	UPLC	UPLC	NNP
cord-014597-66vd2mdu	888	36	(	(	-LRB-
cord-014597-66vd2mdu	888	37	glycan	glycan	NN
cord-014597-66vd2mdu	888	38	species	specie	NNS
cord-014597-66vd2mdu	888	39	)	)	-RRB-
cord-014597-66vd2mdu	888	40	.	.	.
cord-014597-66vd2mdu	889	1	Media	medium	NNS
cord-014597-66vd2mdu	889	2	and	and	CC
cord-014597-66vd2mdu	889	3	feed	feed	NN
cord-014597-66vd2mdu	889	4	compositions	composition	NNS
cord-014597-66vd2mdu	889	5	were	be	VBD
cord-014597-66vd2mdu	889	6	unaffected	unaffected	JJ
cord-014597-66vd2mdu	889	7	by	by	IN
cord-014597-66vd2mdu	889	8	filtration	filtration	NN
cord-014597-66vd2mdu	889	9	through	through	IN
cord-014597-66vd2mdu	889	10	the	the	DT
cord-014597-66vd2mdu	889	11	virus	virus	NN
cord-014597-66vd2mdu	889	12	barrier	barrier	NN
cord-014597-66vd2mdu	889	13	filter	filter	NN
cord-014597-66vd2mdu	889	14	.	.	.
cord-014597-66vd2mdu	890	1	No	no	DT
cord-014597-66vd2mdu	890	2	significant	significant	JJ
cord-014597-66vd2mdu	890	3	differences	difference	NNS
cord-014597-66vd2mdu	890	4	in	in	IN
cord-014597-66vd2mdu	890	5	concentrations	concentration	NNS
cord-014597-66vd2mdu	890	6	were	be	VBD
cord-014597-66vd2mdu	890	7	observed	observe	VBN
cord-014597-66vd2mdu	890	8	with	with	IN
cord-014597-66vd2mdu	890	9	ICP	ICP	NNP
cord-014597-66vd2mdu	890	10	-	-	HYPH
cord-014597-66vd2mdu	890	11	OES	OES	NNP
cord-014597-66vd2mdu	890	12	(	(	-LRB-
cord-014597-66vd2mdu	890	13	trace	trace	NN
cord-014597-66vd2mdu	890	14	metals	metal	NNS
cord-014597-66vd2mdu	890	15	)	)	-RRB-
cord-014597-66vd2mdu	890	16	or	or	CC
cord-014597-66vd2mdu	890	17	HPLC	HPLC	NNP
cord-014597-66vd2mdu	890	18	(	(	-LRB-
cord-014597-66vd2mdu	890	19	amino	amino	NN
cord-014597-66vd2mdu	890	20	acids	acid	NNS
cord-014597-66vd2mdu	890	21	and	and	CC
cord-014597-66vd2mdu	890	22	water	water	NN
cord-014597-66vd2mdu	890	23	soluble	soluble	JJ
cord-014597-66vd2mdu	890	24	vitamins	vitamin	NNS
cord-014597-66vd2mdu	890	25	)	)	-RRB-
cord-014597-66vd2mdu	890	26	.	.	.
cord-014597-66vd2mdu	891	1	NMR	NMR	NNP
cord-014597-66vd2mdu	891	2	showed	show	VBD
cord-014597-66vd2mdu	891	3	no	no	DT
cord-014597-66vd2mdu	891	4	change	change	NN
cord-014597-66vd2mdu	891	5	in	in	IN
cord-014597-66vd2mdu	891	6	the	the	DT
cord-014597-66vd2mdu	891	7	organic	organic	JJ
cord-014597-66vd2mdu	891	8	composition	composition	NN
cord-014597-66vd2mdu	891	9	of	of	IN
cord-014597-66vd2mdu	891	10	the	the	DT
cord-014597-66vd2mdu	891	11	media	medium	NNS
cord-014597-66vd2mdu	891	12	including	include	VBG
cord-014597-66vd2mdu	891	13	poloxamer	poloxamer	NNP
cord-014597-66vd2mdu	891	14	.	.	.
cord-014597-66vd2mdu	892	1	The	the	DT
cord-014597-66vd2mdu	892	2	aromatic	aromatic	JJ
cord-014597-66vd2mdu	892	3	region	region	NN
cord-014597-66vd2mdu	892	4	with	with	IN
cord-014597-66vd2mdu	892	5	vitamin	vitamin	NN
cord-014597-66vd2mdu	892	6	and	and	CC
cord-014597-66vd2mdu	892	7	amino	amino	NN
cord-014597-66vd2mdu	892	8	acid	acid	NN
cord-014597-66vd2mdu	892	9	signals	signal	NNS
cord-014597-66vd2mdu	892	10	is	be	VBZ
cord-014597-66vd2mdu	892	11	shown	show	VBN
cord-014597-66vd2mdu	892	12	(	(	-LRB-
cord-014597-66vd2mdu	892	13	Fig	Fig	NNP
cord-014597-66vd2mdu	892	14	.	.	.
cord-014597-66vd2mdu	892	15	1a	1a	CD
cord-014597-66vd2mdu	892	16	)	)	-RRB-
cord-014597-66vd2mdu	892	17	.	.	.
cord-014597-66vd2mdu	893	1	Cell	cell	NN
cord-014597-66vd2mdu	893	2	cultures	culture	NNS
cord-014597-66vd2mdu	893	3	showed	show	VBD
cord-014597-66vd2mdu	893	4	no	no	DT
cord-014597-66vd2mdu	893	5	differences	difference	NNS
cord-014597-66vd2mdu	893	6	in	in	IN
cord-014597-66vd2mdu	893	7	cell	cell	NN
cord-014597-66vd2mdu	893	8	growth	growth	NN
cord-014597-66vd2mdu	893	9	or	or	CC
cord-014597-66vd2mdu	893	10	titer	titer	NN
cord-014597-66vd2mdu	893	11	,	,	,
cord-014597-66vd2mdu	893	12	in	in	IN
cord-014597-66vd2mdu	893	13	either	either	DT
cord-014597-66vd2mdu	893	14	shake	shake	VB
cord-014597-66vd2mdu	893	15	flasks	flask	NNS
cord-014597-66vd2mdu	893	16	or	or	CC
cord-014597-66vd2mdu	893	17	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	893	18	(	(	-LRB-
cord-014597-66vd2mdu	893	19	Fig	fig	NN
cord-014597-66vd2mdu	893	20	.	.	.
cord-014597-66vd2mdu	893	21	1b	1b	CD
cord-014597-66vd2mdu	893	22	)	)	-RRB-
cord-014597-66vd2mdu	893	23	.	.	.
cord-014597-66vd2mdu	894	1	Cell	cell	NN
cord-014597-66vd2mdu	894	2	viability	viability	NN
cord-014597-66vd2mdu	894	3	was	be	VBD
cord-014597-66vd2mdu	894	4	unaffected	unaffected	JJ
cord-014597-66vd2mdu	894	5	,	,	,
cord-014597-66vd2mdu	894	6	metabolite	metabolite	JJ
cord-014597-66vd2mdu	894	7	levels	level	NNS
cord-014597-66vd2mdu	894	8	were	be	VBD
cord-014597-66vd2mdu	894	9	within	within	IN
cord-014597-66vd2mdu	894	10	limits	limit	NNS
cord-014597-66vd2mdu	894	11	,	,	,
cord-014597-66vd2mdu	894	12	and	and	CC
cord-014597-66vd2mdu	894	13	titer	titer	NN
cord-014597-66vd2mdu	894	14	was	be	VBD
cord-014597-66vd2mdu	894	15	consistent	consistent	JJ
cord-014597-66vd2mdu	894	16	.	.	.
cord-014597-66vd2mdu	895	1	The	the	DT
cord-014597-66vd2mdu	895	2	protein	protein	NN
cord-014597-66vd2mdu	895	3	quality	quality	NN
cord-014597-66vd2mdu	895	4	of	of	IN
cord-014597-66vd2mdu	895	5	the	the	DT
cord-014597-66vd2mdu	895	6	secreted	secrete	VBN
cord-014597-66vd2mdu	895	7	antibodies	antibody	NNS
cord-014597-66vd2mdu	895	8	showed	show	VBD
cord-014597-66vd2mdu	895	9	no	no	DT
cord-014597-66vd2mdu	895	10	differences	difference	NNS
cord-014597-66vd2mdu	895	11	in	in	IN
cord-014597-66vd2mdu	895	12	the	the	DT
cord-014597-66vd2mdu	895	13	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	895	14	pattern	pattern	NN
cord-014597-66vd2mdu	895	15	(	(	-LRB-
cord-014597-66vd2mdu	895	16	Fig	Fig	NNP
cord-014597-66vd2mdu	895	17	.	.	.
cord-014597-66vd2mdu	895	18	1c	1c	NNP
cord-014597-66vd2mdu	895	19	)	)	-RRB-
cord-014597-66vd2mdu	895	20	,	,	,
cord-014597-66vd2mdu	895	21	amount	amount	NN
cord-014597-66vd2mdu	895	22	of	of	IN
cord-014597-66vd2mdu	895	23	aggregates	aggregate	NNS
cord-014597-66vd2mdu	895	24	or	or	CC
cord-014597-66vd2mdu	895	25	charge	charge	NN
cord-014597-66vd2mdu	895	26	variants	variant	NNS
cord-014597-66vd2mdu	895	27	.	.	.
cord-014597-66vd2mdu	896	1	The	the	DT
cord-014597-66vd2mdu	896	2	risk	risk	NN
cord-014597-66vd2mdu	896	3	of	of	IN
cord-014597-66vd2mdu	896	4	virus	virus	NN
cord-014597-66vd2mdu	896	5	contamination	contamination	NN
cord-014597-66vd2mdu	896	6	in	in	IN
cord-014597-66vd2mdu	896	7	the	the	DT
cord-014597-66vd2mdu	896	8	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	896	9	remains	remain	VBZ
cord-014597-66vd2mdu	896	10	a	a	DT
cord-014597-66vd2mdu	896	11	concern	concern	NN
cord-014597-66vd2mdu	896	12	for	for	IN
cord-014597-66vd2mdu	896	13	biotherapeutic	biotherapeutic	JJ
cord-014597-66vd2mdu	896	14	manufacturers	manufacturer	NNS
cord-014597-66vd2mdu	896	15	as	as	IN
cord-014597-66vd2mdu	896	16	there	there	EX
cord-014597-66vd2mdu	896	17	is	be	VBZ
cord-014597-66vd2mdu	896	18	no	no	DT
cord-014597-66vd2mdu	896	19	universal	universal	JJ
cord-014597-66vd2mdu	896	20	technology	technology	NN
cord-014597-66vd2mdu	896	21	that	that	WDT
cord-014597-66vd2mdu	896	22	provides	provide	VBZ
cord-014597-66vd2mdu	896	23	a	a	DT
cord-014597-66vd2mdu	896	24	reliable	reliable	JJ
cord-014597-66vd2mdu	896	25	,	,	,
cord-014597-66vd2mdu	896	26	cost	cost	VBD
cord-014597-66vd2mdu	896	27	effective	effective	JJ
cord-014597-66vd2mdu	896	28	solution	solution	NN
cord-014597-66vd2mdu	896	29	for	for	IN
cord-014597-66vd2mdu	896	30	virus	virus	NN
cord-014597-66vd2mdu	896	31	removal	removal	NN
cord-014597-66vd2mdu	896	32	that	that	WDT
cord-014597-66vd2mdu	896	33	can	can	MD
cord-014597-66vd2mdu	896	34	be	be	VB
cord-014597-66vd2mdu	896	35	applied	apply	VBN
cord-014597-66vd2mdu	896	36	to	to	IN
cord-014597-66vd2mdu	896	37	all	all	DT
cord-014597-66vd2mdu	896	38	components	component	NNS
cord-014597-66vd2mdu	896	39	of	of	IN
cord-014597-66vd2mdu	896	40	cell	cell	NN
cord-014597-66vd2mdu	896	41	culture	culture	NN
cord-014597-66vd2mdu	896	42	media	medium	NNS
cord-014597-66vd2mdu	896	43	.	.	.
cord-014597-66vd2mdu	897	1	This	this	DT
cord-014597-66vd2mdu	897	2	study	study	NN
cord-014597-66vd2mdu	897	3	evaluated	evaluate	VBD
cord-014597-66vd2mdu	897	4	the	the	DT
cord-014597-66vd2mdu	897	5	Viresolve	Viresolve	NNP
cord-014597-66vd2mdu	897	6	®	®	.
cord-014597-66vd2mdu	897	7	Barrier	Barrier	NNP
cord-014597-66vd2mdu	897	8	filter	filter	NN
cord-014597-66vd2mdu	897	9	that	that	WDT
cord-014597-66vd2mdu	897	10	provides	provide	VBZ
cord-014597-66vd2mdu	897	11	an	an	DT
cord-014597-66vd2mdu	897	12	efficient	efficient	JJ
cord-014597-66vd2mdu	897	13	and	and	CC
cord-014597-66vd2mdu	897	14	easy	easy	JJ
cord-014597-66vd2mdu	897	15	way	way	NN
cord-014597-66vd2mdu	897	16	to	to	TO
cord-014597-66vd2mdu	897	17	protect	protect	VB
cord-014597-66vd2mdu	897	18	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	897	19	from	from	IN
cord-014597-66vd2mdu	897	20	adventitious	adventitious	JJ
cord-014597-66vd2mdu	897	21	virus	virus	NN
cord-014597-66vd2mdu	897	22	contamination	contamination	NN
cord-014597-66vd2mdu	897	23	.	.	.
cord-014597-66vd2mdu	898	1	Study	study	NN
cord-014597-66vd2mdu	898	2	results	result	NNS
cord-014597-66vd2mdu	898	3	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	898	4	that	that	IN
cord-014597-66vd2mdu	898	5	media	medium	NNS
cord-014597-66vd2mdu	898	6	and	and	CC
cord-014597-66vd2mdu	898	7	feed	feed	NN
cord-014597-66vd2mdu	898	8	compositions	composition	NNS
cord-014597-66vd2mdu	898	9	,	,	,
cord-014597-66vd2mdu	898	10	cell	cell	NN
cord-014597-66vd2mdu	898	11	culture	culture	NN
cord-014597-66vd2mdu	898	12	performance	performance	NN
cord-014597-66vd2mdu	898	13	,	,	,
cord-014597-66vd2mdu	898	14	and	and	CC
cord-014597-66vd2mdu	898	15	product	product	NN
cord-014597-66vd2mdu	898	16	quality	quality	NN
cord-014597-66vd2mdu	898	17	were	be	VBD
cord-014597-66vd2mdu	898	18	unaffected	unaffected	JJ
cord-014597-66vd2mdu	898	19	by	by	IN
cord-014597-66vd2mdu	898	20	filtration	filtration	NN
cord-014597-66vd2mdu	898	21	through	through	IN
cord-014597-66vd2mdu	898	22	the	the	DT
cord-014597-66vd2mdu	898	23	Viresolve	Viresolve	NNP
cord-014597-66vd2mdu	898	24	®	®	.
cord-014597-66vd2mdu	898	25	Barrier	Barrier	NNP
cord-014597-66vd2mdu	898	26	filter	filter	NN
cord-014597-66vd2mdu	898	27	.	.	.
cord-014597-66vd2mdu	899	1	Implementation	implementation	NN
cord-014597-66vd2mdu	899	2	of	of	IN
cord-014597-66vd2mdu	899	3	Vire	Vire	NNP
cord-014597-66vd2mdu	899	4	-	-	HYPH
cord-014597-66vd2mdu	899	5	solve	solve	VB
cord-014597-66vd2mdu	900	1	®	®	,
cord-014597-66vd2mdu	900	2	Barrier	Barrier	NNP
cord-014597-66vd2mdu	900	3	filters	filter	NNS
cord-014597-66vd2mdu	900	4	provides	provide	VBZ
cord-014597-66vd2mdu	900	5	efficient	efficient	JJ
cord-014597-66vd2mdu	900	6	filtration	filtration	NN
cord-014597-66vd2mdu	900	7	performance	performance	NN
cord-014597-66vd2mdu	900	8	,	,	,
cord-014597-66vd2mdu	900	9	high	high	JJ
cord-014597-66vd2mdu	900	10	virus	virus	NN
cord-014597-66vd2mdu	900	11	retention	retention	NN
cord-014597-66vd2mdu	900	12	,	,	,
cord-014597-66vd2mdu	900	13	and	and	CC
cord-014597-66vd2mdu	900	14	minimal	minimal	JJ
cord-014597-66vd2mdu	900	15	cell	cell	NN
cord-014597-66vd2mdu	900	16	culture	culture	NN
cord-014597-66vd2mdu	900	17	impact	impact	NN
cord-014597-66vd2mdu	900	18	and	and	CC
cord-014597-66vd2mdu	900	19	offers	offer	VBZ
cord-014597-66vd2mdu	900	20	a	a	DT
cord-014597-66vd2mdu	900	21	viable	viable	JJ
cord-014597-66vd2mdu	900	22	option	option	NN
cord-014597-66vd2mdu	900	23	to	to	TO
cord-014597-66vd2mdu	900	24	improve	improve	VB
cord-014597-66vd2mdu	900	25	the	the	DT
cord-014597-66vd2mdu	900	26	overall	overall	JJ
cord-014597-66vd2mdu	900	27	virus	virus	NN
cord-014597-66vd2mdu	900	28	risk	risk	NN
cord-014597-66vd2mdu	900	29	mitigation	mitigation	NN
cord-014597-66vd2mdu	900	30	strategy	strategy	NN
cord-014597-66vd2mdu	900	31	for	for	IN
cord-014597-66vd2mdu	900	32	the	the	DT
cord-014597-66vd2mdu	900	33	manufacture	manufacture	NN
cord-014597-66vd2mdu	900	34	of	of	IN
cord-014597-66vd2mdu	900	35	biotherapeutics	biotherapeutic	NNS
cord-014597-66vd2mdu	900	36	.	.	.
cord-014597-66vd2mdu	901	1	b	b	LS
cord-014597-66vd2mdu	901	2	Tracking	tracking	NN
cord-014597-66vd2mdu	901	3	of	of	IN
cord-014597-66vd2mdu	901	4	process	process	NN
cord-014597-66vd2mdu	901	5	conditions	condition	NNS
cord-014597-66vd2mdu	901	6	together	together	RB
cord-014597-66vd2mdu	901	7	with	with	IN
cord-014597-66vd2mdu	901	8	online	online	JJ
cord-014597-66vd2mdu	901	9	and	and	CC
cord-014597-66vd2mdu	901	10	offline	offline	JJ
cord-014597-66vd2mdu	901	11	performance	performance	NN
cord-014597-66vd2mdu	901	12	analytics	analytic	NNS
cord-014597-66vd2mdu	901	13	.	.	.
cord-014597-66vd2mdu	902	1	The	the	DT
cord-014597-66vd2mdu	902	2	system	system	NN
cord-014597-66vd2mdu	902	3	allows	allow	VBZ
cord-014597-66vd2mdu	902	4	to	to	TO
cord-014597-66vd2mdu	902	5	flexibly	flexibly	RB
cord-014597-66vd2mdu	902	6	define	define	VB
cord-014597-66vd2mdu	902	7	tracked	track	VBN
cord-014597-66vd2mdu	902	8	parameters	parameter	NNS
cord-014597-66vd2mdu	902	9	and	and	CC
cord-014597-66vd2mdu	902	10	select	select	VB
cord-014597-66vd2mdu	902	11	optimal	optimal	JJ
cord-014597-66vd2mdu	902	12	process	process	NN
cord-014597-66vd2mdu	902	13	conditions	condition	NNS
cord-014597-66vd2mdu	902	14	.	.	.
cord-014597-66vd2mdu	903	1	c	c	LS
cord-014597-66vd2mdu	903	2	Comparison	comparison	NN
cord-014597-66vd2mdu	903	3	of	of	IN
cord-014597-66vd2mdu	903	4	process	process	NN
cord-014597-66vd2mdu	903	5	performance	performance	NN
cord-014597-66vd2mdu	903	6	across	across	IN
cord-014597-66vd2mdu	903	7	different	different	JJ
cord-014597-66vd2mdu	903	8	reactor	reactor	NN
cord-014597-66vd2mdu	903	9	scales	scale	NNS
cord-014597-66vd2mdu	903	10	.	.	.
cord-014597-66vd2mdu	904	1	The	the	DT
cord-014597-66vd2mdu	904	2	open	open	JJ
cord-014597-66vd2mdu	904	3	architecture	architecture	NN
cord-014597-66vd2mdu	904	4	makes	make	VBZ
cord-014597-66vd2mdu	904	5	Genedata	Genedata	NNP
cord-014597-66vd2mdu	904	6	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	904	7	a	a	DT
cord-014597-66vd2mdu	904	8	provider	provider	NN
cord-014597-66vd2mdu	904	9	agnostic	agnostic	JJ
cord-014597-66vd2mdu	904	10	system	system	NN
cord-014597-66vd2mdu	904	11	which	which	WDT
cord-014597-66vd2mdu	904	12	allows	allow	VBZ
cord-014597-66vd2mdu	904	13	to	to	TO
cord-014597-66vd2mdu	904	14	aggregate	aggregate	VB
cord-014597-66vd2mdu	904	15	and	and	CC
cord-014597-66vd2mdu	904	16	compare	compare	VB
cord-014597-66vd2mdu	904	17	data	datum	NNS
cord-014597-66vd2mdu	904	18	regardless	regardless	RB
cord-014597-66vd2mdu	904	19	of	of	IN
cord-014597-66vd2mdu	904	20	provider	provider	NN
cord-014597-66vd2mdu	904	21	Background	Background	NNP
cord-014597-66vd2mdu	904	22	Bi	Bi	NNP
cord-014597-66vd2mdu	904	23	-	-	:
cord-014597-66vd2mdu	904	24	and	and	CC
cord-014597-66vd2mdu	904	25	multi	multi	JJ
cord-014597-66vd2mdu	904	26	-	-	JJ
cord-014597-66vd2mdu	904	27	specific	specific	JJ
cord-014597-66vd2mdu	904	28	antibodies	antibody	NNS
cord-014597-66vd2mdu	904	29	,	,	,
cord-014597-66vd2mdu	904	30	antibody	antibody	NN
cord-014597-66vd2mdu	904	31	-	-	HYPH
cord-014597-66vd2mdu	904	32	cytokine	cytokine	NN
cord-014597-66vd2mdu	904	33	fusion	fusion	NN
cord-014597-66vd2mdu	904	34	proteins	protein	NNS
cord-014597-66vd2mdu	904	35	,	,	,
cord-014597-66vd2mdu	904	36	nonimmunoglobulin	nonimmunoglobulin	NNP
cord-014597-66vd2mdu	904	37	scaffolds	scaffold	NNS
cord-014597-66vd2mdu	904	38	,	,	,
cord-014597-66vd2mdu	904	39	chimeric	chimeric	JJ
cord-014597-66vd2mdu	904	40	antigen	antigen	NN
cord-014597-66vd2mdu	904	41	receptors	receptor	NNS
cord-014597-66vd2mdu	904	42	(	(	-LRB-
cord-014597-66vd2mdu	904	43	CARs	car	NNS
cord-014597-66vd2mdu	904	44	)	)	-RRB-
cord-014597-66vd2mdu	904	45	,	,	,
cord-014597-66vd2mdu	904	46	engineered	engineer	VBN
cord-014597-66vd2mdu	904	47	T	t	NN
cord-014597-66vd2mdu	904	48	-	-	HYPH
cord-014597-66vd2mdu	904	49	cell	cell	NN
cord-014597-66vd2mdu	904	50	receptors	receptor	NNS
cord-014597-66vd2mdu	904	51	(	(	-LRB-
cord-014597-66vd2mdu	904	52	TCRs	TCRs	NNP
cord-014597-66vd2mdu	904	53	)	)	-RRB-
cord-014597-66vd2mdu	904	54	and	and	CC
cord-014597-66vd2mdu	904	55	TCR	TCR	NNP
cord-014597-66vd2mdu	904	56	-	-	HYPH
cord-014597-66vd2mdu	904	57	based	base	VBN
cord-014597-66vd2mdu	904	58	bispecific	bispecific	NN
cord-014597-66vd2mdu	904	59	constructs	construct	NNS
cord-014597-66vd2mdu	904	60	can	can	MD
cord-014597-66vd2mdu	904	61	provide	provide	VB
cord-014597-66vd2mdu	904	62	significant	significant	JJ
cord-014597-66vd2mdu	904	63	advantages	advantage	NNS
cord-014597-66vd2mdu	904	64	for	for	IN
cord-014597-66vd2mdu	904	65	use	use	NN
cord-014597-66vd2mdu	904	66	in	in	IN
cord-014597-66vd2mdu	904	67	cancer	cancer	NN
cord-014597-66vd2mdu	904	68	immunotherapy	immunotherapy	NN
cord-014597-66vd2mdu	904	69	.	.	.
cord-014597-66vd2mdu	905	1	However	however	RB
cord-014597-66vd2mdu	905	2	,	,	,
cord-014597-66vd2mdu	905	3	as	as	IN
cord-014597-66vd2mdu	905	4	highly	highly	RB
cord-014597-66vd2mdu	905	5	engineered	engineer	VBN
cord-014597-66vd2mdu	905	6	molecules	molecule	NNS
cord-014597-66vd2mdu	905	7	they	-PRON-	PRP
cord-014597-66vd2mdu	905	8	pose	pose	VBP
cord-014597-66vd2mdu	905	9	new	new	JJ
cord-014597-66vd2mdu	905	10	challenges	challenge	NNS
cord-014597-66vd2mdu	905	11	in	in	IN
cord-014597-66vd2mdu	905	12	design	design	NN
cord-014597-66vd2mdu	905	13	,	,	,
cord-014597-66vd2mdu	905	14	engineering	engineering	NN
cord-014597-66vd2mdu	905	15	,	,	,
cord-014597-66vd2mdu	905	16	cloning	cloning	NN
cord-014597-66vd2mdu	905	17	,	,	,
cord-014597-66vd2mdu	905	18	expression	expression	NN
cord-014597-66vd2mdu	905	19	,	,	,
cord-014597-66vd2mdu	905	20	purification	purification	NN
cord-014597-66vd2mdu	905	21	,	,	,
cord-014597-66vd2mdu	905	22	and	and	CC
cord-014597-66vd2mdu	905	23	analytics	analytic	NNS
cord-014597-66vd2mdu	905	24	.	.	.
cord-014597-66vd2mdu	906	1	We	-PRON-	PRP
cord-014597-66vd2mdu	906	2	have	have	VBP
cord-014597-66vd2mdu	906	3	thus	thus	RB
cord-014597-66vd2mdu	906	4	implemented	implement	VBN
cord-014597-66vd2mdu	906	5	an	an	DT
cord-014597-66vd2mdu	906	6	infrastructure	infrastructure	NN
cord-014597-66vd2mdu	906	7	that	that	WDT
cord-014597-66vd2mdu	906	8	addresses	address	VBZ
cord-014597-66vd2mdu	906	9	these	these	DT
cord-014597-66vd2mdu	906	10	challenges	challenge	NNS
cord-014597-66vd2mdu	906	11	and	and	CC
cord-014597-66vd2mdu	906	12	enables	enable	VBZ
cord-014597-66vd2mdu	906	13	the	the	DT
cord-014597-66vd2mdu	906	14	industrialization	industrialization	NN
cord-014597-66vd2mdu	906	15	of	of	IN
cord-014597-66vd2mdu	906	16	these	these	DT
cord-014597-66vd2mdu	906	17	various	various	JJ
cord-014597-66vd2mdu	906	18	novel	novel	JJ
cord-014597-66vd2mdu	906	19	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	906	20	platforms	platform	NNS
cord-014597-66vd2mdu	906	21	.	.	.
cord-014597-66vd2mdu	907	1	In	in	IN
cord-014597-66vd2mdu	907	2	close	close	JJ
cord-014597-66vd2mdu	907	3	collaboration	collaboration	NN
cord-014597-66vd2mdu	907	4	with	with	IN
cord-014597-66vd2mdu	907	5	leading	lead	VBG
cord-014597-66vd2mdu	907	6	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	907	7	companies	company	NNS
cord-014597-66vd2mdu	907	8	,	,	,
cord-014597-66vd2mdu	907	9	we	-PRON-	PRP
cord-014597-66vd2mdu	907	10	implemented	implement	VBD
cord-014597-66vd2mdu	907	11	a	a	DT
cord-014597-66vd2mdu	907	12	workflow	workflow	NN
cord-014597-66vd2mdu	907	13	,	,	,
cord-014597-66vd2mdu	907	14	data	datum	NNS
cord-014597-66vd2mdu	907	15	management	management	NN
cord-014597-66vd2mdu	907	16	and	and	CC
cord-014597-66vd2mdu	907	17	analysis	analysis	NN
cord-014597-66vd2mdu	907	18	support	support	NN
cord-014597-66vd2mdu	907	19	system	system	NN
cord-014597-66vd2mdu	907	20	,	,	,
cord-014597-66vd2mdu	907	21	Genedata	Genedata	NNP
cord-014597-66vd2mdu	907	22	Biologics	Biologics	NNP
cord-014597-66vd2mdu	907	23	™	™	NN
cord-014597-66vd2mdu	907	24	,	,	,
cord-014597-66vd2mdu	907	25	enabling	enable	VBG
cord-014597-66vd2mdu	907	26	the	the	DT
cord-014597-66vd2mdu	907	27	automated	automate	VBN
cord-014597-66vd2mdu	907	28	design	design	NN
cord-014597-66vd2mdu	907	29	,	,	,
cord-014597-66vd2mdu	907	30	screening	screening	NN
cord-014597-66vd2mdu	907	31	,	,	,
cord-014597-66vd2mdu	907	32	and	and	CC
cord-014597-66vd2mdu	907	33	expression	expression	NN
cord-014597-66vd2mdu	907	34	of	of	IN
cord-014597-66vd2mdu	907	35	large	large	JJ
cord-014597-66vd2mdu	907	36	panels	panel	NNS
cord-014597-66vd2mdu	907	37	of	of	IN
cord-014597-66vd2mdu	907	38	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	907	39	candidates	candidate	NNS
cord-014597-66vd2mdu	907	40	using	use	VBG
cord-014597-66vd2mdu	907	41	these	these	DT
cord-014597-66vd2mdu	907	42	novel	novel	JJ
cord-014597-66vd2mdu	907	43	technologies	technology	NNS
cord-014597-66vd2mdu	907	44	.	.	.
cord-014597-66vd2mdu	908	1	We	-PRON-	PRP
cord-014597-66vd2mdu	908	2	have	have	VBP
cord-014597-66vd2mdu	908	3	also	also	RB
cord-014597-66vd2mdu	908	4	built	build	VBN
cord-014597-66vd2mdu	908	5	tools	tool	NNS
cord-014597-66vd2mdu	908	6	for	for	IN
cord-014597-66vd2mdu	908	7	developability	developability	NN
cord-014597-66vd2mdu	908	8	and	and	CC
cord-014597-66vd2mdu	908	9	manufacturability	manufacturability	NN
cord-014597-66vd2mdu	908	10	assessments	assessment	NNS
cord-014597-66vd2mdu	908	11	of	of	IN
cord-014597-66vd2mdu	908	12	these	these	DT
cord-014597-66vd2mdu	908	13	complex	complex	JJ
cord-014597-66vd2mdu	908	14	molecules	molecule	NNS
cord-014597-66vd2mdu	908	15	.	.	.
cord-014597-66vd2mdu	909	1	We	-PRON-	PRP
cord-014597-66vd2mdu	909	2	have	have	VBP
cord-014597-66vd2mdu	909	3	ensured	ensure	VBN
cord-014597-66vd2mdu	909	4	that	that	IN
cord-014597-66vd2mdu	909	5	there	there	EX
cord-014597-66vd2mdu	909	6	is	be	VBZ
cord-014597-66vd2mdu	909	7	a	a	DT
cord-014597-66vd2mdu	909	8	seamless	seamless	JJ
cord-014597-66vd2mdu	909	9	integration	integration	NN
cord-014597-66vd2mdu	909	10	of	of	IN
cord-014597-66vd2mdu	909	11	all	all	DT
cord-014597-66vd2mdu	909	12	data	datum	NNS
cord-014597-66vd2mdu	909	13	generated	generate	VBN
cord-014597-66vd2mdu	909	14	and	and	CC
cord-014597-66vd2mdu	909	15	that	that	IN
cord-014597-66vd2mdu	909	16	functionalities	functionality	NNS
cord-014597-66vd2mdu	909	17	such	such	JJ
cord-014597-66vd2mdu	909	18	as	as	IN
cord-014597-66vd2mdu	909	19	bulk	bulk	JJ
cord-014597-66vd2mdu	909	20	protein	protein	NN
cord-014597-66vd2mdu	909	21	and	and	CC
cord-014597-66vd2mdu	909	22	vector	vector	NN
cord-014597-66vd2mdu	909	23	generation	generation	NN
cord-014597-66vd2mdu	909	24	using	use	VBG
cord-014597-66vd2mdu	909	25	our	-PRON-	PRP$
cord-014597-66vd2mdu	909	26	in	in	IN
cord-014597-66vd2mdu	909	27	silico	silico	NN
cord-014597-66vd2mdu	909	28	cloning	clone	VBG
cord-014597-66vd2mdu	909	29	engine	engine	NN
cord-014597-66vd2mdu	909	30	,	,	,
cord-014597-66vd2mdu	909	31	configurable	configurable	JJ
cord-014597-66vd2mdu	909	32	library	library	NN
cord-014597-66vd2mdu	909	33	of	of	IN
cord-014597-66vd2mdu	909	34	template	template	NN
cord-014597-66vd2mdu	909	35	vectors	vector	NNS
cord-014597-66vd2mdu	909	36	and	and	CC
cord-014597-66vd2mdu	909	37	cloning	clone	VBG
cord-014597-66vd2mdu	909	38	strategies	strategy	NNS
cord-014597-66vd2mdu	909	39	,	,	,
cord-014597-66vd2mdu	909	40	fully	fully	RB
cord-014597-66vd2mdu	909	41	annotated	annotate	VBN
cord-014597-66vd2mdu	909	42	in	in	IN
cord-014597-66vd2mdu	909	43	silico	silico	NN
cord-014597-66vd2mdu	909	44	protein	protein	NN
cord-014597-66vd2mdu	909	45	molecules	molecule	NNS
cord-014597-66vd2mdu	909	46	and	and	CC
cord-014597-66vd2mdu	909	47	DNA	dna	NN
cord-014597-66vd2mdu	909	48	constructs	construct	NNS
cord-014597-66vd2mdu	909	49	,	,	,
cord-014597-66vd2mdu	909	50	and	and	CC
cord-014597-66vd2mdu	909	51	DNA	dna	NN
cord-014597-66vd2mdu	909	52	synthesis	synthesis	NN
cord-014597-66vd2mdu	909	53	verification	verification	NN
cord-014597-66vd2mdu	909	54	support	support	NN
cord-014597-66vd2mdu	909	55	,	,	,
cord-014597-66vd2mdu	909	56	can	can	MD
cord-014597-66vd2mdu	909	57	be	be	VB
cord-014597-66vd2mdu	909	58	used	use	VBN
cord-014597-66vd2mdu	909	59	for	for	IN
cord-014597-66vd2mdu	909	60	the	the	DT
cord-014597-66vd2mdu	909	61	newest	new	JJS
cord-014597-66vd2mdu	909	62	protein	protein	NN
cord-014597-66vd2mdu	909	63	formats	format	NNS
cord-014597-66vd2mdu	909	64	and	and	CC
cord-014597-66vd2mdu	909	65	molecule	molecule	NN
cord-014597-66vd2mdu	909	66	topologies	topology	NNS
cord-014597-66vd2mdu	909	67	.	.	.
cord-014597-66vd2mdu	910	1	We	-PRON-	PRP
cord-014597-66vd2mdu	910	2	implemented	implement	VBD
cord-014597-66vd2mdu	910	3	data	data	NN
cord-014597-66vd2mdu	910	4	structures	structure	NNS
cord-014597-66vd2mdu	910	5	and	and	CC
cord-014597-66vd2mdu	910	6	data	datum	NNS
cord-014597-66vd2mdu	910	7	handling	handle	VBG
cord-014597-66vd2mdu	910	8	systems	system	NNS
cord-014597-66vd2mdu	910	9	,	,	,
cord-014597-66vd2mdu	910	10	which	which	WDT
cord-014597-66vd2mdu	910	11	mirror	mirror	VBP
cord-014597-66vd2mdu	910	12	how	how	WRB
cord-014597-66vd2mdu	910	13	these	these	DT
cord-014597-66vd2mdu	910	14	complex	complex	JJ
cord-014597-66vd2mdu	910	15	next	next	JJ
cord-014597-66vd2mdu	910	16	-	-	HYPH
cord-014597-66vd2mdu	910	17	generation	generation	NN
cord-014597-66vd2mdu	910	18	biologics	biologic	NNS
cord-014597-66vd2mdu	910	19	molecules	molecule	NNS
cord-014597-66vd2mdu	910	20	and	and	CC
cord-014597-66vd2mdu	910	21	cell	cell	NN
cord-014597-66vd2mdu	910	22	lines	line	NNS
cord-014597-66vd2mdu	910	23	are	be	VBP
cord-014597-66vd2mdu	910	24	being	be	VBG
cord-014597-66vd2mdu	910	25	designed	design	VBN
cord-014597-66vd2mdu	910	26	,	,	,
cord-014597-66vd2mdu	910	27	screened	screen	VBN
cord-014597-66vd2mdu	910	28	,	,	,
cord-014597-66vd2mdu	910	29	and	and	CC
cord-014597-66vd2mdu	910	30	analyzed	analyze	VBD
cord-014597-66vd2mdu	910	31	.	.	.
cord-014597-66vd2mdu	911	1	The	the	DT
cord-014597-66vd2mdu	911	2	result	result	NN
cord-014597-66vd2mdu	911	3	successfully	successfully	RB
cord-014597-66vd2mdu	911	4	addresses	address	VBZ
cord-014597-66vd2mdu	911	5	workflows	workflow	NNS
cord-014597-66vd2mdu	911	6	for	for	IN
cord-014597-66vd2mdu	911	7	TCR	TCR	NNP
cord-014597-66vd2mdu	911	8	optimization	optimization	NN
cord-014597-66vd2mdu	911	9	and	and	CC
cord-014597-66vd2mdu	911	10	engineering	engineering	NN
cord-014597-66vd2mdu	911	11	.	.	.
cord-014597-66vd2mdu	912	1	We	-PRON-	PRP
cord-014597-66vd2mdu	912	2	exemplified	exemplify	VBD
cord-014597-66vd2mdu	912	3	this	this	DT
cord-014597-66vd2mdu	912	4	with	with	IN
cord-014597-66vd2mdu	912	5	the	the	DT
cord-014597-66vd2mdu	912	6	generation	generation	NN
cord-014597-66vd2mdu	912	7	and	and	CC
cord-014597-66vd2mdu	912	8	evaluation	evaluation	NN
cord-014597-66vd2mdu	912	9	of	of	IN
cord-014597-66vd2mdu	912	10	a	a	DT
cord-014597-66vd2mdu	912	11	panel	panel	NN
cord-014597-66vd2mdu	912	12	of	of	IN
cord-014597-66vd2mdu	912	13	engineered	engineer	VBN
cord-014597-66vd2mdu	912	14	TCRs	tcr	NNS
cord-014597-66vd2mdu	912	15	with	with	IN
cord-014597-66vd2mdu	912	16	an	an	DT
cord-014597-66vd2mdu	912	17	alpha	alpha	NN
cord-014597-66vd2mdu	912	18	chain	chain	NN
cord-014597-66vd2mdu	912	19	CDR3	CDR3	NNP
cord-014597-66vd2mdu	912	20	randomization	randomization	NN
cord-014597-66vd2mdu	912	21	and	and	CC
cord-014597-66vd2mdu	912	22	successfully	successfully	RB
cord-014597-66vd2mdu	912	23	supported	support	VBD
cord-014597-66vd2mdu	912	24	the	the	DT
cord-014597-66vd2mdu	912	25	analysis	analysis	NN
cord-014597-66vd2mdu	912	26	and	and	CC
cord-014597-66vd2mdu	912	27	selection	selection	NN
cord-014597-66vd2mdu	912	28	of	of	IN
cord-014597-66vd2mdu	912	29	beneficial	beneficial	JJ
cord-014597-66vd2mdu	912	30	mutations	mutation	NNS
cord-014597-66vd2mdu	912	31	.	.	.
cord-014597-66vd2mdu	913	1	The	the	DT
cord-014597-66vd2mdu	913	2	system	system	NN
cord-014597-66vd2mdu	913	3	also	also	RB
cord-014597-66vd2mdu	913	4	successfully	successfully	RB
cord-014597-66vd2mdu	913	5	supported	support	VBD
cord-014597-66vd2mdu	913	6	workflows	workflow	NNS
cord-014597-66vd2mdu	913	7	for	for	IN
cord-014597-66vd2mdu	913	8	the	the	DT
cord-014597-66vd2mdu	913	9	design	design	NN
cord-014597-66vd2mdu	913	10	and	and	CC
cord-014597-66vd2mdu	913	11	generation	generation	NN
cord-014597-66vd2mdu	913	12	of	of	IN
cord-014597-66vd2mdu	913	13	a	a	DT
cord-014597-66vd2mdu	913	14	panel	panel	NN
cord-014597-66vd2mdu	913	15	of	of	IN
cord-014597-66vd2mdu	913	16	TCR	TCR	NNP
cord-014597-66vd2mdu	913	17	-	-	HYPH
cord-014597-66vd2mdu	913	18	based	base	VBN
cord-014597-66vd2mdu	913	19	bispecifics	bispecific	NNS
cord-014597-66vd2mdu	914	1	(	(	-LRB-
cord-014597-66vd2mdu	914	2	TCR	TCR	NNP
cord-014597-66vd2mdu	914	3	coupled	couple	VBD
cord-014597-66vd2mdu	914	4	with	with	IN
cord-014597-66vd2mdu	914	5	anti	anti	JJ
cord-014597-66vd2mdu	914	6	-	-	JJ
cord-014597-66vd2mdu	914	7	CD3	cd3	JJ
cord-014597-66vd2mdu	914	8	)	)	-RRB-
cord-014597-66vd2mdu	914	9	using	use	VBG
cord-014597-66vd2mdu	914	10	automated	automate	VBN
cord-014597-66vd2mdu	914	11	molecule	molecule	NN
cord-014597-66vd2mdu	914	12	registration	registration	NN
cord-014597-66vd2mdu	914	13	and	and	CC
cord-014597-66vd2mdu	914	14	in	in	IN
cord-014597-66vd2mdu	914	15	silico	silico	NN
cord-014597-66vd2mdu	914	16	cloning	cloning	NN
cord-014597-66vd2mdu	914	17	tools	tool	NNS
cord-014597-66vd2mdu	914	18	and	and	CC
cord-014597-66vd2mdu	914	19	subsequent	subsequent	JJ
cord-014597-66vd2mdu	914	20	capture	capture	NN
cord-014597-66vd2mdu	914	21	of	of	IN
cord-014597-66vd2mdu	914	22	expression	expression	NN
cord-014597-66vd2mdu	914	23	,	,	,
cord-014597-66vd2mdu	914	24	purification	purification	NN
cord-014597-66vd2mdu	914	25	,	,	,
cord-014597-66vd2mdu	914	26	and	and	CC
cord-014597-66vd2mdu	914	27	functional	functional	JJ
cord-014597-66vd2mdu	914	28	and	and	CC
cord-014597-66vd2mdu	914	29	analytical	analytical	JJ
cord-014597-66vd2mdu	914	30	characterization	characterization	NN
cord-014597-66vd2mdu	914	31	data	datum	NNS
cord-014597-66vd2mdu	914	32	.	.	.
cord-014597-66vd2mdu	915	1	On	on	IN
cord-014597-66vd2mdu	915	2	the	the	DT
cord-014597-66vd2mdu	915	3	CAR	car	NN
cord-014597-66vd2mdu	915	4	-	-	HYPH
cord-014597-66vd2mdu	915	5	T	t	NN
cord-014597-66vd2mdu	915	6	cell	cell	NN
cord-014597-66vd2mdu	915	7	front	front	NN
cord-014597-66vd2mdu	915	8	,	,	,
cord-014597-66vd2mdu	915	9	the	the	DT
cord-014597-66vd2mdu	915	10	system	system	NN
cord-014597-66vd2mdu	915	11	is	be	VBZ
cord-014597-66vd2mdu	915	12	able	able	JJ
cord-014597-66vd2mdu	915	13	to	to	TO
cord-014597-66vd2mdu	915	14	provide	provide	VB
cord-014597-66vd2mdu	915	15	traceability	traceability	NN
cord-014597-66vd2mdu	915	16	of	of	IN
cord-014597-66vd2mdu	915	17	the	the	DT
cord-014597-66vd2mdu	915	18	work	work	NN
cord-014597-66vd2mdu	915	19	from	from	IN
cord-014597-66vd2mdu	915	20	antibody	antibody	NN
cord-014597-66vd2mdu	915	21	generation	generation	NN
cord-014597-66vd2mdu	915	22	,	,	,
cord-014597-66vd2mdu	915	23	optimization	optimization	NN
cord-014597-66vd2mdu	915	24	,	,	,
cord-014597-66vd2mdu	915	25	CAR	car	NN
cord-014597-66vd2mdu	915	26	engineering	engineering	NN
cord-014597-66vd2mdu	915	27	(	(	-LRB-
cord-014597-66vd2mdu	915	28	e.g.	e.g.	RB
cord-014597-66vd2mdu	915	29	,	,	,
cord-014597-66vd2mdu	915	30	attachment	attachment	NN
cord-014597-66vd2mdu	915	31	to	to	IN
cord-014597-66vd2mdu	915	32	the	the	DT
cord-014597-66vd2mdu	915	33	scFv	scFv	NNS
cord-014597-66vd2mdu	915	34	with	with	IN
cord-014597-66vd2mdu	915	35	CD3-zeta	cd3-zeta	NN
cord-014597-66vd2mdu	915	36	and	and	CC
cord-014597-66vd2mdu	915	37	co	co	JJ
cord-014597-66vd2mdu	915	38	-	-	JJ
cord-014597-66vd2mdu	915	39	stimulatory	stimulatory	JJ
cord-014597-66vd2mdu	915	40	domains	domain	NNS
cord-014597-66vd2mdu	915	41	to	to	TO
cord-014597-66vd2mdu	915	42	mimic	mimic	VB
cord-014597-66vd2mdu	915	43	the	the	DT
cord-014597-66vd2mdu	915	44	natural	natural	JJ
cord-014597-66vd2mdu	915	45	TCR	TCR	NNP
cord-014597-66vd2mdu	915	46	complex	complex	NN
cord-014597-66vd2mdu	915	47	)	)	-RRB-
cord-014597-66vd2mdu	915	48	to	to	IN
cord-014597-66vd2mdu	915	49	the	the	DT
cord-014597-66vd2mdu	915	50	engineering	engineering	NN
cord-014597-66vd2mdu	915	51	of	of	IN
cord-014597-66vd2mdu	915	52	the	the	DT
cord-014597-66vd2mdu	915	53	T	t	NN
cord-014597-66vd2mdu	915	54	-	-	HYPH
cord-014597-66vd2mdu	915	55	cell	cell	NN
cord-014597-66vd2mdu	915	56	.	.	.
cord-014597-66vd2mdu	916	1	The	the	DT
cord-014597-66vd2mdu	916	2	Genedata	Genedata	NNP
cord-014597-66vd2mdu	916	3	Biologics	Biologics	NNP
cord-014597-66vd2mdu	916	4	platform	platform	NN
cord-014597-66vd2mdu	916	5	successfully	successfully	RB
cord-014597-66vd2mdu	916	6	enabled	enable	VBD
cord-014597-66vd2mdu	916	7	automation	automation	NN
cord-014597-66vd2mdu	916	8	,	,	,
cord-014597-66vd2mdu	916	9	increased	increase	VBN
cord-014597-66vd2mdu	916	10	data	datum	NNS
cord-014597-66vd2mdu	916	11	integrity	integrity	NN
cord-014597-66vd2mdu	916	12	and	and	CC
cord-014597-66vd2mdu	916	13	traceability	traceability	NN
cord-014597-66vd2mdu	916	14	during	during	IN
cord-014597-66vd2mdu	916	15	research	research	NN
cord-014597-66vd2mdu	916	16	and	and	CC
cord-014597-66vd2mdu	916	17	development	development	NN
cord-014597-66vd2mdu	916	18	work	work	NN
cord-014597-66vd2mdu	916	19	,	,	,
cord-014597-66vd2mdu	916	20	and	and	CC
cord-014597-66vd2mdu	916	21	will	will	MD
cord-014597-66vd2mdu	916	22	contribute	contribute	VB
cord-014597-66vd2mdu	916	23	towards	towards	IN
cord-014597-66vd2mdu	916	24	the	the	DT
cord-014597-66vd2mdu	916	25	industrialization	industrialization	NN
cord-014597-66vd2mdu	916	26	of	of	IN
cord-014597-66vd2mdu	916	27	these	these	DT
cord-014597-66vd2mdu	916	28	very	very	RB
cord-014597-66vd2mdu	916	29	exciting	exciting	JJ
cord-014597-66vd2mdu	916	30	novel	novel	JJ
cord-014597-66vd2mdu	916	31	approaches	approach	NNS
cord-014597-66vd2mdu	916	32	for	for	IN
cord-014597-66vd2mdu	916	33	cancer	cancer	NN
cord-014597-66vd2mdu	916	34	immunotherapy	immunotherapy	NN
cord-014597-66vd2mdu	916	35	.	.	.
cord-014597-66vd2mdu	917	1	Optimal	optimal	JJ
cord-014597-66vd2mdu	917	2	selection	selection	NN
cord-014597-66vd2mdu	917	3	of	of	IN
cord-014597-66vd2mdu	917	4	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	917	5	antibodies	antibody	NNS
cord-014597-66vd2mdu	917	6	and	and	CC
cord-014597-66vd2mdu	917	7	production	production	NN
cord-014597-66vd2mdu	917	8	cell	cell	NN
cord-014597-66vd2mdu	917	9	lines	line	NNS
cord-014597-66vd2mdu	917	10	by	by	IN
cord-014597-66vd2mdu	917	11	assessment	assessment	NN
cord-014597-66vd2mdu	917	12	of	of	IN
cord-014597-66vd2mdu	917	13	critical	critical	JJ
cord-014597-66vd2mdu	917	14	quality	quality	NN
cord-014597-66vd2mdu	917	15	attributes	attribute	NNS
cord-014597-66vd2mdu	917	16	and	and	CC
cord-014597-66vd2mdu	917	17	developability	developability	NN
cord-014597-66vd2mdu	917	18	Background	background	NN
cord-014597-66vd2mdu	918	1	The	the	DT
cord-014597-66vd2mdu	918	2	increasing	increase	VBG
cord-014597-66vd2mdu	918	3	cost	cost	NN
cord-014597-66vd2mdu	918	4	of	of	IN
cord-014597-66vd2mdu	918	5	bringing	bring	VBG
cord-014597-66vd2mdu	918	6	a	a	DT
cord-014597-66vd2mdu	918	7	new	new	JJ
cord-014597-66vd2mdu	918	8	drug	drug	NN
cord-014597-66vd2mdu	918	9	to	to	IN
cord-014597-66vd2mdu	918	10	the	the	DT
cord-014597-66vd2mdu	918	11	market	market	NN
cord-014597-66vd2mdu	918	12	has	have	VBZ
cord-014597-66vd2mdu	918	13	put	put	VBN
cord-014597-66vd2mdu	918	14	significant	significant	JJ
cord-014597-66vd2mdu	918	15	pressure	pressure	NN
cord-014597-66vd2mdu	918	16	on	on	IN
cord-014597-66vd2mdu	918	17	biopharma	biopharma	NN
cord-014597-66vd2mdu	918	18	organizations	organization	NNS
cord-014597-66vd2mdu	918	19	.	.	.
cord-014597-66vd2mdu	919	1	To	to	TO
cord-014597-66vd2mdu	919	2	increase	increase	VB
cord-014597-66vd2mdu	919	3	efficiency	efficiency	NN
cord-014597-66vd2mdu	919	4	in	in	IN
cord-014597-66vd2mdu	919	5	R&D	r&d	NN
cord-014597-66vd2mdu	919	6	processes	process	NNS
cord-014597-66vd2mdu	919	7	and	and	CC
cord-014597-66vd2mdu	919	8	reduce	reduce	VB
cord-014597-66vd2mdu	919	9	costs	cost	NNS
cord-014597-66vd2mdu	919	10	,	,	,
cord-014597-66vd2mdu	919	11	organizations	organization	NNS
cord-014597-66vd2mdu	919	12	need	need	VBP
cord-014597-66vd2mdu	919	13	to	to	TO
cord-014597-66vd2mdu	919	14	evaluate	evaluate	VB
cord-014597-66vd2mdu	919	15	potential	potential	JJ
cord-014597-66vd2mdu	919	16	drug	drug	NN
cord-014597-66vd2mdu	919	17	candidates	candidate	NNS
cord-014597-66vd2mdu	919	18	earlier	early	RBR
cord-014597-66vd2mdu	919	19	in	in	IN
cord-014597-66vd2mdu	919	20	the	the	DT
cord-014597-66vd2mdu	919	21	R&D	r&d	NN
cord-014597-66vd2mdu	919	22	process	process	NN
cord-014597-66vd2mdu	919	23	,	,	,
cord-014597-66vd2mdu	919	24	eliminate	eliminate	VB
cord-014597-66vd2mdu	919	25	those	those	DT
cord-014597-66vd2mdu	919	26	with	with	IN
cord-014597-66vd2mdu	919	27	undesirable	undesirable	JJ
cord-014597-66vd2mdu	919	28	characteristics	characteristic	NNS
cord-014597-66vd2mdu	919	29	,	,	,
cord-014597-66vd2mdu	919	30	and	and	CC
cord-014597-66vd2mdu	919	31	focus	focus	VB
cord-014597-66vd2mdu	919	32	on	on	IN
cord-014597-66vd2mdu	919	33	the	the	DT
cord-014597-66vd2mdu	919	34	most	most	RBS
cord-014597-66vd2mdu	919	35	promising	promising	JJ
cord-014597-66vd2mdu	919	36	candidates	candidate	NNS
cord-014597-66vd2mdu	919	37	.	.	.
cord-014597-66vd2mdu	920	1	After	after	IN
cord-014597-66vd2mdu	920	2	designing	design	VBG
cord-014597-66vd2mdu	920	3	and	and	CC
cord-014597-66vd2mdu	920	4	thorough	thorough	JJ
cord-014597-66vd2mdu	920	5	testing	testing	NN
cord-014597-66vd2mdu	920	6	of	of	IN
cord-014597-66vd2mdu	920	7	successful	successful	JJ
cord-014597-66vd2mdu	920	8	candidates	candidate	NNS
cord-014597-66vd2mdu	920	9	,	,	,
cord-014597-66vd2mdu	920	10	efficient	efficient	JJ
cord-014597-66vd2mdu	920	11	production	production	NN
cord-014597-66vd2mdu	920	12	of	of	IN
cord-014597-66vd2mdu	920	13	new	new	JJ
cord-014597-66vd2mdu	920	14	biological	biological	JJ
cord-014597-66vd2mdu	920	15	entities	entity	NNS
cord-014597-66vd2mdu	920	16	in	in	IN
cord-014597-66vd2mdu	920	17	mammalian	mammalian	JJ
cord-014597-66vd2mdu	920	18	cell	cell	NN
cord-014597-66vd2mdu	920	19	lines	line	NNS
cord-014597-66vd2mdu	920	20	is	be	VBZ
cord-014597-66vd2mdu	920	21	necessary	necessary	JJ
cord-014597-66vd2mdu	920	22	.	.	.
cord-014597-66vd2mdu	921	1	The	the	DT
cord-014597-66vd2mdu	921	2	main	main	JJ
cord-014597-66vd2mdu	921	3	goal	goal	NN
cord-014597-66vd2mdu	921	4	here	here	RB
cord-014597-66vd2mdu	921	5	is	be	VBZ
cord-014597-66vd2mdu	921	6	to	to	TO
cord-014597-66vd2mdu	921	7	find	find	VB
cord-014597-66vd2mdu	921	8	a	a	DT
cord-014597-66vd2mdu	921	9	suitable	suitable	JJ
cord-014597-66vd2mdu	921	10	cell	cell	NN
cord-014597-66vd2mdu	921	11	line	line	NN
cord-014597-66vd2mdu	921	12	and	and	CC
cord-014597-66vd2mdu	921	13	optimal	optimal	JJ
cord-014597-66vd2mdu	921	14	upstream	upstream	RB
cord-014597-66vd2mdu	921	15	and	and	CC
cord-014597-66vd2mdu	921	16	downstream	downstream	JJ
cord-014597-66vd2mdu	921	17	processing	processing	NN
cord-014597-66vd2mdu	921	18	conditions	condition	NNS
cord-014597-66vd2mdu	921	19	that	that	WDT
cord-014597-66vd2mdu	921	20	not	not	RB
cord-014597-66vd2mdu	921	21	only	only	RB
cord-014597-66vd2mdu	921	22	lead	lead	VB
cord-014597-66vd2mdu	921	23	to	to	IN
cord-014597-66vd2mdu	921	24	a	a	DT
cord-014597-66vd2mdu	921	25	satisfactory	satisfactory	JJ
cord-014597-66vd2mdu	921	26	product	product	NN
cord-014597-66vd2mdu	921	27	yield	yield	NN
cord-014597-66vd2mdu	921	28	,	,	,
cord-014597-66vd2mdu	921	29	but	but	CC
cord-014597-66vd2mdu	921	30	also	also	RB
cord-014597-66vd2mdu	921	31	to	to	IN
cord-014597-66vd2mdu	921	32	a	a	DT
cord-014597-66vd2mdu	921	33	product	product	NN
cord-014597-66vd2mdu	921	34	with	with	IN
cord-014597-66vd2mdu	921	35	the	the	DT
cord-014597-66vd2mdu	921	36	desired	desire	VBN
cord-014597-66vd2mdu	921	37	biochemical	biochemical	JJ
cord-014597-66vd2mdu	921	38	properties	property	NNS
cord-014597-66vd2mdu	921	39	.	.	.
cord-014597-66vd2mdu	922	1	The	the	DT
cord-014597-66vd2mdu	922	2	evaluation	evaluation	NN
cord-014597-66vd2mdu	922	3	of	of	IN
cord-014597-66vd2mdu	922	4	production	production	NN
cord-014597-66vd2mdu	922	5	cell	cell	NN
cord-014597-66vd2mdu	922	6	lines	line	NNS
cord-014597-66vd2mdu	922	7	,	,	,
cord-014597-66vd2mdu	922	8	processes	process	NNS
cord-014597-66vd2mdu	922	9	,	,	,
cord-014597-66vd2mdu	922	10	and	and	CC
cord-014597-66vd2mdu	922	11	product	product	NN
cord-014597-66vd2mdu	922	12	quality	quality	NN
cord-014597-66vd2mdu	922	13	attributes	attribute	NNS
cord-014597-66vd2mdu	922	14	is	be	VBZ
cord-014597-66vd2mdu	922	15	performed	perform	VBN
cord-014597-66vd2mdu	922	16	earlier	early	RBR
cord-014597-66vd2mdu	922	17	and	and	CC
cord-014597-66vd2mdu	922	18	in	in	IN
cord-014597-66vd2mdu	922	19	higher	high	JJR
cord-014597-66vd2mdu	922	20	throughput	throughput	NN
cord-014597-66vd2mdu	922	21	for	for	IN
cord-014597-66vd2mdu	922	22	an	an	DT
cord-014597-66vd2mdu	922	23	increasing	increase	VBG
cord-014597-66vd2mdu	922	24	number	number	NN
cord-014597-66vd2mdu	922	25	of	of	IN
cord-014597-66vd2mdu	922	26	drug	drug	NN
cord-014597-66vd2mdu	922	27	candidates	candidate	NNS
cord-014597-66vd2mdu	922	28	.	.	.
cord-014597-66vd2mdu	923	1	In	in	IN
cord-014597-66vd2mdu	923	2	addition	addition	NN
cord-014597-66vd2mdu	923	3	,	,	,
cord-014597-66vd2mdu	923	4	new	new	JJ
cord-014597-66vd2mdu	923	5	methods	method	NNS
cord-014597-66vd2mdu	923	6	in	in	IN
cord-014597-66vd2mdu	923	7	molecular	molecular	JJ
cord-014597-66vd2mdu	923	8	and	and	CC
cord-014597-66vd2mdu	923	9	cell	cell	NN
cord-014597-66vd2mdu	923	10	biology	biology	NN
cord-014597-66vd2mdu	923	11	(	(	-LRB-
cord-014597-66vd2mdu	923	12	e.g.	e.g.	RB
cord-014597-66vd2mdu	923	13	,	,	,
cord-014597-66vd2mdu	923	14	novel	novel	JJ
cord-014597-66vd2mdu	923	15	genome	genome	NN
cord-014597-66vd2mdu	923	16	engineering	engineering	NN
cord-014597-66vd2mdu	923	17	approaches	approach	NNS
cord-014597-66vd2mdu	923	18	such	such	JJ
cord-014597-66vd2mdu	923	19	as	as	IN
cord-014597-66vd2mdu	923	20	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	923	21	/	/	SYM
cord-014597-66vd2mdu	923	22	Cas9	Cas9	NNP
cord-014597-66vd2mdu	923	23	)	)	-RRB-
cord-014597-66vd2mdu	923	24	,	,	,
cord-014597-66vd2mdu	923	25	in	in	IN
cord-014597-66vd2mdu	923	26	analytics	analytic	NNS
cord-014597-66vd2mdu	923	27	[	[	-LRB-
cord-014597-66vd2mdu	923	28	e.g.	e.g.	RB
cord-014597-66vd2mdu	923	29	,	,	,
cord-014597-66vd2mdu	923	30	Process	Process	NNP
cord-014597-66vd2mdu	923	31	Analytical	Analytical	NNP
cord-014597-66vd2mdu	923	32	Techniques	Techniques	NNPS
cord-014597-66vd2mdu	923	33	(	(	-LRB-
cord-014597-66vd2mdu	923	34	PATs	PATs	NNP
cord-014597-66vd2mdu	923	35	)	)	-RRB-
cord-014597-66vd2mdu	923	36	]	]	-RRB-
cord-014597-66vd2mdu	923	37	,	,	,
cord-014597-66vd2mdu	923	38	in	in	IN
cord-014597-66vd2mdu	923	39	process	process	NN
cord-014597-66vd2mdu	923	40	miniaturization	miniaturization	NN
cord-014597-66vd2mdu	923	41	,	,	,
cord-014597-66vd2mdu	923	42	and	and	CC
cord-014597-66vd2mdu	923	43	in	in	IN
cord-014597-66vd2mdu	923	44	automation	automation	NN
cord-014597-66vd2mdu	923	45	promise	promise	NN
cord-014597-66vd2mdu	923	46	to	to	TO
cord-014597-66vd2mdu	923	47	make	make	VB
cord-014597-66vd2mdu	923	48	process	process	NN
cord-014597-66vd2mdu	923	49	development	development	NN
cord-014597-66vd2mdu	923	50	more	more	RBR
cord-014597-66vd2mdu	923	51	efficient	efficient	JJ
cord-014597-66vd2mdu	923	52	.	.	.
cord-014597-66vd2mdu	924	1	However	however	RB
cord-014597-66vd2mdu	924	2	,	,	,
cord-014597-66vd2mdu	924	3	the	the	DT
cord-014597-66vd2mdu	924	4	management	management	NN
cord-014597-66vd2mdu	924	5	and	and	CC
cord-014597-66vd2mdu	924	6	analysis	analysis	NN
cord-014597-66vd2mdu	924	7	of	of	IN
cord-014597-66vd2mdu	924	8	the	the	DT
cord-014597-66vd2mdu	924	9	increasing	increase	VBG
cord-014597-66vd2mdu	924	10	amount	amount	NN
cord-014597-66vd2mdu	924	11	of	of	IN
cord-014597-66vd2mdu	924	12	experimental	experimental	JJ
cord-014597-66vd2mdu	924	13	data	datum	NNS
cord-014597-66vd2mdu	924	14	during	during	IN
cord-014597-66vd2mdu	924	15	candidate	candidate	NN
cord-014597-66vd2mdu	924	16	selection	selection	NN
cord-014597-66vd2mdu	924	17	and	and	CC
cord-014597-66vd2mdu	924	18	cell	cell	NN
cord-014597-66vd2mdu	924	19	line	line	NN
cord-014597-66vd2mdu	924	20	and	and	CC
cord-014597-66vd2mdu	924	21	process	process	NN
cord-014597-66vd2mdu	924	22	development	development	NN
cord-014597-66vd2mdu	924	23	has	have	VBZ
cord-014597-66vd2mdu	924	24	become	become	VBN
cord-014597-66vd2mdu	924	25	a	a	DT
cord-014597-66vd2mdu	924	26	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	924	27	.	.	.
cord-014597-66vd2mdu	925	1	In	in	IN
cord-014597-66vd2mdu	925	2	addition	addition	NN
cord-014597-66vd2mdu	925	3	,	,	,
cord-014597-66vd2mdu	925	4	quality	quality	NN
cord-014597-66vd2mdu	925	5	-	-	HYPH
cord-014597-66vd2mdu	925	6	compromising	compromise	VBG
cord-014597-66vd2mdu	925	7	steps	step	NNS
cord-014597-66vd2mdu	925	8	in	in	IN
cord-014597-66vd2mdu	925	9	biopharma	biopharma	NN
cord-014597-66vd2mdu	925	10	organizations	organization	NNS
cord-014597-66vd2mdu	925	11	can	can	MD
cord-014597-66vd2mdu	925	12	negatively	negatively	RB
cord-014597-66vd2mdu	925	13	impact	impact	VB
cord-014597-66vd2mdu	925	14	the	the	DT
cord-014597-66vd2mdu	925	15	cost	cost	NN
cord-014597-66vd2mdu	925	16	of	of	IN
cord-014597-66vd2mdu	925	17	goods	good	NNS
cord-014597-66vd2mdu	925	18	and	and	CC
cord-014597-66vd2mdu	925	19	substantially	substantially	RB
cord-014597-66vd2mdu	925	20	prolong	prolong	VB
cord-014597-66vd2mdu	925	21	the	the	DT
cord-014597-66vd2mdu	925	22	drug	drug	NN
cord-014597-66vd2mdu	925	23	candidate	candidate	NN
cord-014597-66vd2mdu	925	24	's	's	POS
cord-014597-66vd2mdu	925	25	time	time	NN
cord-014597-66vd2mdu	925	26	to	to	IN
cord-014597-66vd2mdu	925	27	market	market	NN
cord-014597-66vd2mdu	925	28	.	.	.
cord-014597-66vd2mdu	926	1	Therefore	therefore	RB
cord-014597-66vd2mdu	926	2	,	,	,
cord-014597-66vd2mdu	926	3	systems	system	NNS
cord-014597-66vd2mdu	926	4	for	for	IN
cord-014597-66vd2mdu	926	5	integrated	integrated	JJ
cord-014597-66vd2mdu	926	6	management	management	NN
cord-014597-66vd2mdu	926	7	and	and	CC
cord-014597-66vd2mdu	926	8	analysis	analysis	NN
cord-014597-66vd2mdu	926	9	of	of	IN
cord-014597-66vd2mdu	926	10	wellstructured	wellstructured	NNP
cord-014597-66vd2mdu	926	11	and	and	CC
cord-014597-66vd2mdu	926	12	curated	curate	VBN
cord-014597-66vd2mdu	926	13	data	datum	NNS
cord-014597-66vd2mdu	926	14	that	that	WDT
cord-014597-66vd2mdu	926	15	comprehensively	comprehensively	RB
cord-014597-66vd2mdu	926	16	integrate	integrate	VBP
cord-014597-66vd2mdu	926	17	molecule	molecule	NN
cord-014597-66vd2mdu	926	18	and	and	CC
cord-014597-66vd2mdu	926	19	sample	sample	NN
cord-014597-66vd2mdu	926	20	information	information	NN
cord-014597-66vd2mdu	926	21	,	,	,
cord-014597-66vd2mdu	926	22	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	926	23	process	process	NN
cord-014597-66vd2mdu	926	24	parameters	parameter	NNS
cord-014597-66vd2mdu	926	25	,	,	,
cord-014597-66vd2mdu	926	26	and	and	CC
cord-014597-66vd2mdu	926	27	process	process	NN
cord-014597-66vd2mdu	926	28	and	and	CC
cord-014597-66vd2mdu	926	29	product	product	NN
cord-014597-66vd2mdu	926	30	quality	quality	NN
cord-014597-66vd2mdu	926	31	attributes	attribute	NNS
cord-014597-66vd2mdu	926	32	are	be	VBP
cord-014597-66vd2mdu	926	33	needed	need	VBN
cord-014597-66vd2mdu	926	34	.	.	.
cord-014597-66vd2mdu	927	1	Critical	critical	JJ
cord-014597-66vd2mdu	927	2	quality	quality	NN
cord-014597-66vd2mdu	927	3	attribute	attribute	NN
cord-014597-66vd2mdu	927	4	(	(	-LRB-
cord-014597-66vd2mdu	927	5	CQA	CQA	NNP
cord-014597-66vd2mdu	927	6	)	)	-RRB-
cord-014597-66vd2mdu	927	7	assessment	assessment	NN
cord-014597-66vd2mdu	927	8	should	should	MD
cord-014597-66vd2mdu	927	9	be	be	VB
cord-014597-66vd2mdu	927	10	enabled	enable	VBN
cord-014597-66vd2mdu	927	11	along	along	IN
cord-014597-66vd2mdu	927	12	the	the	DT
cord-014597-66vd2mdu	927	13	whole	whole	JJ
cord-014597-66vd2mdu	927	14	bioprocess	bioprocess	NNP
cord-014597-66vd2mdu	927	15	development	development	NNP
cord-014597-66vd2mdu	927	16	workflow	workflow	NN
cord-014597-66vd2mdu	927	17	,	,	,
cord-014597-66vd2mdu	927	18	including	include	VBG
cord-014597-66vd2mdu	927	19	cell	cell	NN
cord-014597-66vd2mdu	927	20	line	line	NN
cord-014597-66vd2mdu	927	21	development	development	NN
cord-014597-66vd2mdu	927	22	,	,	,
cord-014597-66vd2mdu	927	23	upstream	upstream	JJ
cord-014597-66vd2mdu	927	24	and	and	CC
cord-014597-66vd2mdu	927	25	downstream	downstream	JJ
cord-014597-66vd2mdu	927	26	process	process	NN
cord-014597-66vd2mdu	927	27	development	development	NN
cord-014597-66vd2mdu	927	28	,	,	,
cord-014597-66vd2mdu	927	29	as	as	RB
cord-014597-66vd2mdu	927	30	well	well	RB
cord-014597-66vd2mdu	927	31	as	as	IN
cord-014597-66vd2mdu	927	32	analytical	analytical	JJ
cord-014597-66vd2mdu	927	33	and	and	CC
cord-014597-66vd2mdu	927	34	formulation	formulation	NN
cord-014597-66vd2mdu	927	35	development	development	NN
cord-014597-66vd2mdu	927	36	.	.	.
cord-014597-66vd2mdu	928	1	We	-PRON-	PRP
cord-014597-66vd2mdu	928	2	have	have	VBP
cord-014597-66vd2mdu	928	3	developed	develop	VBN
cord-014597-66vd2mdu	928	4	a	a	DT
cord-014597-66vd2mdu	928	5	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	928	6	platform	platform	NN
cord-014597-66vd2mdu	928	7	,	,	,
cord-014597-66vd2mdu	928	8	Genedata	Genedata	NNP
cord-014597-66vd2mdu	928	9	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	928	10	™	™	NN
cord-014597-66vd2mdu	928	11	,	,	,
cord-014597-66vd2mdu	928	12	which	which	WDT
cord-014597-66vd2mdu	928	13	supports	support	VBZ
cord-014597-66vd2mdu	928	14	drug	drug	NN
cord-014597-66vd2mdu	928	15	candidate	candidate	NN
cord-014597-66vd2mdu	928	16	developability	developability	NN
cord-014597-66vd2mdu	928	17	and	and	CC
cord-014597-66vd2mdu	928	18	manufacturability	manufacturability	NN
cord-014597-66vd2mdu	928	19	assessment	assessment	NN
cord-014597-66vd2mdu	928	20	and	and	CC
cord-014597-66vd2mdu	928	21	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	928	22	development	development	NN
cord-014597-66vd2mdu	928	23	.	.	.
cord-014597-66vd2mdu	929	1	The	the	DT
cord-014597-66vd2mdu	929	2	platform	platform	NN
cord-014597-66vd2mdu	929	3	captures	capture	VBZ
cord-014597-66vd2mdu	929	4	and	and	CC
cord-014597-66vd2mdu	929	5	structures	structure	NNS
cord-014597-66vd2mdu	929	6	the	the	DT
cord-014597-66vd2mdu	929	7	cell	cell	NN
cord-014597-66vd2mdu	929	8	line	line	NN
cord-014597-66vd2mdu	929	9	and	and	CC
cord-014597-66vd2mdu	929	10	process	process	NN
cord-014597-66vd2mdu	929	11	parameters	parameter	NNS
cord-014597-66vd2mdu	929	12	together	together	RB
cord-014597-66vd2mdu	929	13	with	with	IN
cord-014597-66vd2mdu	929	14	analytical	analytical	JJ
cord-014597-66vd2mdu	929	15	data	datum	NNS
cord-014597-66vd2mdu	929	16	for	for	IN
cord-014597-66vd2mdu	929	17	cell	cell	NN
cord-014597-66vd2mdu	929	18	lines	line	NNS
cord-014597-66vd2mdu	929	19	,	,	,
cord-014597-66vd2mdu	929	20	processes	process	NNS
cord-014597-66vd2mdu	929	21	,	,	,
cord-014597-66vd2mdu	929	22	and	and	CC
cord-014597-66vd2mdu	929	23	protein	protein	NN
cord-014597-66vd2mdu	929	24	products	product	NNS
cord-014597-66vd2mdu	929	25	.	.	.
cord-014597-66vd2mdu	930	1	The	the	DT
cord-014597-66vd2mdu	930	2	protein	protein	NN
cord-014597-66vd2mdu	930	3	analytical	analytical	JJ
cord-014597-66vd2mdu	930	4	data	datum	NNS
cord-014597-66vd2mdu	930	5	being	be	VBG
cord-014597-66vd2mdu	930	6	tracked	track	VBN
cord-014597-66vd2mdu	930	7	include	include	VBP
cord-014597-66vd2mdu	930	8	biological	biological	JJ
cord-014597-66vd2mdu	930	9	data	datum	NNS
cord-014597-66vd2mdu	930	10	(	(	-LRB-
cord-014597-66vd2mdu	930	11	such	such	JJ
cord-014597-66vd2mdu	930	12	as	as	IN
cord-014597-66vd2mdu	930	13	bioactivity	bioactivity	NN
cord-014597-66vd2mdu	930	14	,	,	,
cord-014597-66vd2mdu	930	15	immunogenicity	immunogenicity	NN
cord-014597-66vd2mdu	930	16	)	)	-RRB-
cord-014597-66vd2mdu	930	17	,	,	,
cord-014597-66vd2mdu	930	18	and	and	CC
cord-014597-66vd2mdu	930	19	physicochemical	physicochemical	JJ
cord-014597-66vd2mdu	930	20	properties	property	NNS
cord-014597-66vd2mdu	930	21	.	.	.
cord-014597-66vd2mdu	931	1	These	these	DT
cord-014597-66vd2mdu	931	2	properties	property	NNS
cord-014597-66vd2mdu	931	3	include	include	VBP
cord-014597-66vd2mdu	931	4	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	931	5	,	,	,
cord-014597-66vd2mdu	931	6	chemical	chemical	NN
cord-014597-66vd2mdu	931	7	liabilities	liability	NNS
cord-014597-66vd2mdu	931	8	(	(	-LRB-
cord-014597-66vd2mdu	931	9	such	such	JJ
cord-014597-66vd2mdu	931	10	as	as	IN
cord-014597-66vd2mdu	931	11	deamidation	deamidation	NN
cord-014597-66vd2mdu	931	12	and	and	CC
cord-014597-66vd2mdu	931	13	oxidation	oxidation	NN
cord-014597-66vd2mdu	931	14	)	)	-RRB-
cord-014597-66vd2mdu	931	15	,	,	,
cord-014597-66vd2mdu	931	16	aggregation	aggregation	NN
cord-014597-66vd2mdu	931	17	,	,	,
cord-014597-66vd2mdu	931	18	stability	stability	NN
cord-014597-66vd2mdu	931	19	under	under	IN
cord-014597-66vd2mdu	931	20	different	different	JJ
cord-014597-66vd2mdu	931	21	conditions	condition	NNS
cord-014597-66vd2mdu	931	22	(	(	-LRB-
cord-014597-66vd2mdu	931	23	low	low	JJ
cord-014597-66vd2mdu	931	24	pH	ph	NN
cord-014597-66vd2mdu	931	25	,	,	,
cord-014597-66vd2mdu	931	26	low	low	JJ
cord-014597-66vd2mdu	931	27	and	and	CC
cord-014597-66vd2mdu	931	28	high	high	JJ
cord-014597-66vd2mdu	931	29	temperature	temperature	NN
cord-014597-66vd2mdu	931	30	)	)	-RRB-
cord-014597-66vd2mdu	931	31	,	,	,
cord-014597-66vd2mdu	931	32	solubility	solubility	NN
cord-014597-66vd2mdu	931	33	,	,	,
cord-014597-66vd2mdu	931	34	and	and	CC
cord-014597-66vd2mdu	931	35	impurities	impurity	NNS
cord-014597-66vd2mdu	931	36	.	.	.
cord-014597-66vd2mdu	932	1	Genedata	Genedata	NNP
cord-014597-66vd2mdu	932	2	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	932	3	™	™	NN
cord-014597-66vd2mdu	932	4	simplifies	simplify	VBZ
cord-014597-66vd2mdu	932	5	and	and	CC
cord-014597-66vd2mdu	932	6	streamlines	streamline	VBZ
cord-014597-66vd2mdu	932	7	laborious	laborious	JJ
cord-014597-66vd2mdu	932	8	,	,	,
cord-014597-66vd2mdu	932	9	manual	manual	JJ
cord-014597-66vd2mdu	932	10	process	process	NN
cord-014597-66vd2mdu	932	11	and	and	CC
cord-014597-66vd2mdu	932	12	supports	support	VBZ
cord-014597-66vd2mdu	932	13	tools	tool	NNS
cord-014597-66vd2mdu	932	14	for	for	IN
cord-014597-66vd2mdu	932	15	molecule	molecule	NN
cord-014597-66vd2mdu	932	16	,	,	,
cord-014597-66vd2mdu	932	17	clone	clone	NN
cord-014597-66vd2mdu	932	18	and	and	CC
cord-014597-66vd2mdu	932	19	process	process	NN
cord-014597-66vd2mdu	932	20	selection	selection	NN
cord-014597-66vd2mdu	932	21	.	.	.
cord-014597-66vd2mdu	933	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	933	2	,	,	,
cord-014597-66vd2mdu	933	3	the	the	DT
cord-014597-66vd2mdu	933	4	platform	platform	NN
cord-014597-66vd2mdu	933	5	allows	allow	VBZ
cord-014597-66vd2mdu	933	6	for	for	IN
cord-014597-66vd2mdu	933	7	seamless	seamless	JJ
cord-014597-66vd2mdu	933	8	integration	integration	NN
cord-014597-66vd2mdu	933	9	with	with	IN
cord-014597-66vd2mdu	933	10	laboratory	laboratory	NN
cord-014597-66vd2mdu	933	11	instruments	instrument	NNS
cord-014597-66vd2mdu	933	12	,	,	,
cord-014597-66vd2mdu	933	13	statistical	statistical	JJ
cord-014597-66vd2mdu	933	14	software	software	NN
cord-014597-66vd2mdu	933	15	packages	package	NNS
cord-014597-66vd2mdu	933	16	,	,	,
cord-014597-66vd2mdu	933	17	and	and	CC
cord-014597-66vd2mdu	933	18	custom	custom	NN
cord-014597-66vd2mdu	933	19	solutions	solution	NNS
cord-014597-66vd2mdu	933	20	.	.	.
cord-014597-66vd2mdu	934	1	Here	here	RB
cord-014597-66vd2mdu	934	2	,	,	,
cord-014597-66vd2mdu	934	3	we	-PRON-	PRP
cord-014597-66vd2mdu	934	4	present	present	VBP
cord-014597-66vd2mdu	934	5	use	use	NN
cord-014597-66vd2mdu	934	6	cases	case	NNS
cord-014597-66vd2mdu	934	7	showing	show	VBG
cord-014597-66vd2mdu	934	8	how	how	WRB
cord-014597-66vd2mdu	934	9	to	to	TO
cord-014597-66vd2mdu	934	10	identify	identify	VB
cord-014597-66vd2mdu	934	11	and	and	CC
cord-014597-66vd2mdu	934	12	annotate	annotate	VB
cord-014597-66vd2mdu	934	13	liability	liability	NN
cord-014597-66vd2mdu	934	14	sites	site	NNS
cord-014597-66vd2mdu	934	15	prone	prone	JJ
cord-014597-66vd2mdu	934	16	to	to	IN
cord-014597-66vd2mdu	934	17	chemical	chemical	JJ
cord-014597-66vd2mdu	934	18	modifications	modification	NNS
cord-014597-66vd2mdu	934	19	(	(	-LRB-
cord-014597-66vd2mdu	934	20	Fig	Fig	NNP
cord-014597-66vd2mdu	934	21	.	.	.
cord-014597-66vd2mdu	934	22	1a	1a	CD
cord-014597-66vd2mdu	934	23	)	)	-RRB-
cord-014597-66vd2mdu	934	24	and	and	CC
cord-014597-66vd2mdu	934	25	how	how	WRB
cord-014597-66vd2mdu	934	26	to	to	TO
cord-014597-66vd2mdu	934	27	monitor	monitor	VB
cord-014597-66vd2mdu	934	28	CQAs	cqa	NNS
cord-014597-66vd2mdu	934	29	of	of	IN
cord-014597-66vd2mdu	934	30	molecules	molecule	NNS
cord-014597-66vd2mdu	934	31	allowing	allow	VBG
cord-014597-66vd2mdu	934	32	to	to	TO
cord-014597-66vd2mdu	934	33	assess	assess	VB
cord-014597-66vd2mdu	934	34	developability	developability	NN
cord-014597-66vd2mdu	934	35	more	more	RBR
cord-014597-66vd2mdu	934	36	efficiently	efficiently	RB
cord-014597-66vd2mdu	934	37	.	.	.
cord-014597-66vd2mdu	935	1	We	-PRON-	PRP
cord-014597-66vd2mdu	935	2	show	show	VBP
cord-014597-66vd2mdu	935	3	how	how	WRB
cord-014597-66vd2mdu	935	4	the	the	DT
cord-014597-66vd2mdu	935	5	analytical	analytical	JJ
cord-014597-66vd2mdu	935	6	data	datum	NNS
cord-014597-66vd2mdu	935	7	generated	generate	VBN
cord-014597-66vd2mdu	935	8	in	in	IN
cord-014597-66vd2mdu	935	9	the	the	DT
cord-014597-66vd2mdu	935	10	course	course	NN
cord-014597-66vd2mdu	935	11	of	of	IN
cord-014597-66vd2mdu	935	12	a	a	DT
cord-014597-66vd2mdu	935	13	developability	developability	NN
cord-014597-66vd2mdu	935	14	assessment	assessment	NN
cord-014597-66vd2mdu	935	15	are	be	VBP
cord-014597-66vd2mdu	935	16	compiled	compile	VBN
cord-014597-66vd2mdu	935	17	to	to	TO
cord-014597-66vd2mdu	935	18	select	select	VB
cord-014597-66vd2mdu	935	19	the	the	DT
cord-014597-66vd2mdu	935	20	best	good	JJS
cord-014597-66vd2mdu	935	21	drug	drug	NN
cord-014597-66vd2mdu	935	22	candidate	candidate	NN
cord-014597-66vd2mdu	935	23	(	(	-LRB-
cord-014597-66vd2mdu	935	24	Fig	Fig	NNP
cord-014597-66vd2mdu	935	25	.	.	.
cord-014597-66vd2mdu	935	26	1b	1b	CD
cord-014597-66vd2mdu	935	27	)	)	-RRB-
cord-014597-66vd2mdu	935	28	.	.	.
cord-014597-66vd2mdu	936	1	Implemented	implement	VBN
cord-014597-66vd2mdu	936	2	traffic	traffic	NN
cord-014597-66vd2mdu	936	3	-	-	HYPH
cord-014597-66vd2mdu	936	4	light	light	NN
cord-014597-66vd2mdu	936	5	systems	system	NNS
cord-014597-66vd2mdu	936	6	indicate	indicate	VBP
cord-014597-66vd2mdu	936	7	where	where	WRB
cord-014597-66vd2mdu	936	8	molecules	molecule	NNS
cord-014597-66vd2mdu	936	9	harbor	harbor	VBP
cord-014597-66vd2mdu	936	10	issues	issue	NNS
cord-014597-66vd2mdu	936	11	such	such	JJ
cord-014597-66vd2mdu	936	12	as	as	IN
cord-014597-66vd2mdu	936	13	in	in	IN
cord-014597-66vd2mdu	936	14	case	case	NN
cord-014597-66vd2mdu	936	15	of	of	IN
cord-014597-66vd2mdu	936	16	the	the	DT
cord-014597-66vd2mdu	936	17	antibody	antibody	NN
cord-014597-66vd2mdu	936	18	TPP-86	TPP-86	NNP
cord-014597-66vd2mdu	936	19	,	,	,
cord-014597-66vd2mdu	936	20	which	which	WDT
cord-014597-66vd2mdu	936	21	is	be	VBZ
cord-014597-66vd2mdu	936	22	compromised	compromise	VBN
cord-014597-66vd2mdu	936	23	by	by	IN
cord-014597-66vd2mdu	936	24	low	low	JJ
cord-014597-66vd2mdu	936	25	temperature	temperature	NN
cord-014597-66vd2mdu	936	26	and	and	CC
cord-014597-66vd2mdu	936	27	repeated	repeat	VBD
cord-014597-66vd2mdu	936	28	freeze	freeze	NN
cord-014597-66vd2mdu	936	29	-	-	HYPH
cord-014597-66vd2mdu	936	30	thaw	thaw	NN
cord-014597-66vd2mdu	936	31	operations	operation	NNS
cord-014597-66vd2mdu	936	32	.	.	.
cord-014597-66vd2mdu	937	1	The	the	DT
cord-014597-66vd2mdu	937	2	same	same	JJ
cord-014597-66vd2mdu	937	3	assessment	assessment	NN
cord-014597-66vd2mdu	937	4	views	view	NNS
cord-014597-66vd2mdu	937	5	can	can	MD
cord-014597-66vd2mdu	937	6	also	also	RB
cord-014597-66vd2mdu	937	7	be	be	VB
cord-014597-66vd2mdu	937	8	applied	apply	VBN
cord-014597-66vd2mdu	937	9	on	on	IN
cord-014597-66vd2mdu	937	10	batches	batch	NNS
cord-014597-66vd2mdu	937	11	and	and	CC
cord-014597-66vd2mdu	937	12	cell	cell	NN
cord-014597-66vd2mdu	937	13	lines	line	NNS
cord-014597-66vd2mdu	937	14	.	.	.
cord-014597-66vd2mdu	938	1	The	the	DT
cord-014597-66vd2mdu	938	2	underlying	underlie	VBG
cord-014597-66vd2mdu	938	3	data	datum	NNS
cord-014597-66vd2mdu	938	4	can	can	MD
cord-014597-66vd2mdu	938	5	be	be	VB
cord-014597-66vd2mdu	938	6	visualized	visualize	VBN
cord-014597-66vd2mdu	938	7	graphically	graphically	RB
cord-014597-66vd2mdu	938	8	.	.	.
cord-014597-66vd2mdu	939	1	As	as	IN
cord-014597-66vd2mdu	939	2	an	an	DT
cord-014597-66vd2mdu	939	3	example	example	NN
cord-014597-66vd2mdu	939	4	,	,	,
cord-014597-66vd2mdu	939	5	we	-PRON-	PRP
cord-014597-66vd2mdu	939	6	show	show	VBP
cord-014597-66vd2mdu	939	7	glycan	glycan	NN
cord-014597-66vd2mdu	939	8	types	type	NNS
cord-014597-66vd2mdu	939	9	of	of	IN
cord-014597-66vd2mdu	939	10	products	product	NNS
cord-014597-66vd2mdu	939	11	obtained	obtain	VBN
cord-014597-66vd2mdu	939	12	from	from	IN
cord-014597-66vd2mdu	939	13	different	different	JJ
cord-014597-66vd2mdu	939	14	cell	cell	NN
cord-014597-66vd2mdu	939	15	line	line	NN
cord-014597-66vd2mdu	939	16	clones	clone	NNS
cord-014597-66vd2mdu	939	17	generated	generate	VBN
cord-014597-66vd2mdu	939	18	in	in	IN
cord-014597-66vd2mdu	939	19	a	a	DT
cord-014597-66vd2mdu	939	20	cell	cell	NN
cord-014597-66vd2mdu	939	21	line	line	NN
cord-014597-66vd2mdu	939	22	development	development	NN
cord-014597-66vd2mdu	939	23	campaign	campaign	NN
cord-014597-66vd2mdu	939	24	for	for	IN
cord-014597-66vd2mdu	939	25	the	the	DT
cord-014597-66vd2mdu	939	26	molecule	molecule	NN
cord-014597-66vd2mdu	939	27	TPP-86	TPP-86	NNP
cord-014597-66vd2mdu	939	28	(	(	-LRB-
cord-014597-66vd2mdu	939	29	Fig	Fig	NNP
cord-014597-66vd2mdu	939	30	.	.	.
cord-014597-66vd2mdu	939	31	1c	1c	CD
cord-014597-66vd2mdu	939	32	)	)	-RRB-
cord-014597-66vd2mdu	939	33	.	.	.
cord-014597-66vd2mdu	940	1	Even	even	RB
cord-014597-66vd2mdu	940	2	though	though	IN
cord-014597-66vd2mdu	940	3	the	the	DT
cord-014597-66vd2mdu	940	4	selected	select	VBN
cord-014597-66vd2mdu	940	5	clone	clone	NN
cord-014597-66vd2mdu	940	6	CLI-35	CLI-35	NNP
cord-014597-66vd2mdu	940	7	meets	meet	VBZ
cord-014597-66vd2mdu	940	8	the	the	DT
cord-014597-66vd2mdu	940	9	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	940	10	criteria	criterion	NNS
cord-014597-66vd2mdu	940	11	(	(	-LRB-
cord-014597-66vd2mdu	940	12	e.	e.	NNP
cord-014597-66vd2mdu	940	13	g.	g.	NNP
cord-014597-66vd2mdu	940	14	,	,	,
cord-014597-66vd2mdu	940	15	<	<	NNP
cord-014597-66vd2mdu	940	16	13	13	CD
cord-014597-66vd2mdu	940	17	%	%	NN
cord-014597-66vd2mdu	940	18	afucosylation	afucosylation	NN
cord-014597-66vd2mdu	940	19	,	,	,
cord-014597-66vd2mdu	940	20	<	<	XX
cord-014597-66vd2mdu	940	21	40	40	CD
cord-014597-66vd2mdu	940	22	%	%	NN
cord-014597-66vd2mdu	940	23	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	940	24	,	,	,
cord-014597-66vd2mdu	940	25	<	<	XX
cord-014597-66vd2mdu	940	26	2	2	CD
cord-014597-66vd2mdu	940	27	%	%	NN
cord-014597-66vd2mdu	940	28	sialylation	sialylation	NN
cord-014597-66vd2mdu	940	29	)	)	-RRB-
cord-014597-66vd2mdu	940	30	,	,	,
cord-014597-66vd2mdu	940	31	the	the	DT
cord-014597-66vd2mdu	940	32	produced	produce	VBN
cord-014597-66vd2mdu	940	33	Next	Next	NNP
cord-014597-66vd2mdu	940	34	-	-	HYPH
cord-014597-66vd2mdu	940	35	generation	generation	NN
cord-014597-66vd2mdu	940	36	biologics	biologic	NNS
cord-014597-66vd2mdu	940	37	molecules	molecule	NNS
cord-014597-66vd2mdu	940	38	are	be	VBP
cord-014597-66vd2mdu	940	39	composed	compose	VBN
cord-014597-66vd2mdu	940	40	from	from	IN
cord-014597-66vd2mdu	940	41	a	a	DT
cord-014597-66vd2mdu	940	42	number	number	NN
cord-014597-66vd2mdu	940	43	of	of	IN
cord-014597-66vd2mdu	940	44	specific	specific	JJ
cord-014597-66vd2mdu	940	45	subdomains	subdomain	NNS
cord-014597-66vd2mdu	940	46	.	.	.
cord-014597-66vd2mdu	941	1	Each	each	DT
cord-014597-66vd2mdu	941	2	type	type	NN
cord-014597-66vd2mdu	941	3	of	of	IN
cord-014597-66vd2mdu	941	4	molecule	molecule	NN
cord-014597-66vd2mdu	941	5	is	be	VBZ
cord-014597-66vd2mdu	941	6	composed	compose	VBN
cord-014597-66vd2mdu	941	7	of	of	IN
cord-014597-66vd2mdu	941	8	a	a	DT
cord-014597-66vd2mdu	941	9	specific	specific	JJ
cord-014597-66vd2mdu	941	10	set	set	NN
cord-014597-66vd2mdu	941	11	of	of	IN
cord-014597-66vd2mdu	941	12	domains	domain	NNS
cord-014597-66vd2mdu	941	13	,	,	,
cord-014597-66vd2mdu	941	14	which	which	WDT
cord-014597-66vd2mdu	941	15	must	must	MD
cord-014597-66vd2mdu	941	16	be	be	VB
cord-014597-66vd2mdu	941	17	mirrored	mirror	VBN
cord-014597-66vd2mdu	941	18	in	in	IN
cord-014597-66vd2mdu	941	19	the	the	DT
cord-014597-66vd2mdu	941	20	registration	registration	NN
cord-014597-66vd2mdu	941	21	and	and	CC
cord-014597-66vd2mdu	941	22	further	further	JJ
cord-014597-66vd2mdu	941	23	research	research	NN
cord-014597-66vd2mdu	941	24	and	and	CC
cord-014597-66vd2mdu	941	25	development	development	NN
cord-014597-66vd2mdu	941	26	workflow	workflow	NN
cord-014597-66vd2mdu	941	27	.	.	.
cord-014597-66vd2mdu	942	1	Molecule	molecule	NN
cord-014597-66vd2mdu	942	2	registration	registration	NN
cord-014597-66vd2mdu	942	3	and	and	CC
cord-014597-66vd2mdu	942	4	hit	hit	NN
cord-014597-66vd2mdu	942	5	-	-	HYPH
cord-014597-66vd2mdu	942	6	selection	selection	NN
cord-014597-66vd2mdu	943	1	using	use	VBG
cord-014597-66vd2mdu	943	2	data	datum	NNS
cord-014597-66vd2mdu	943	3	from	from	IN
cord-014597-66vd2mdu	943	4	a	a	DT
cord-014597-66vd2mdu	943	5	number	number	NN
cord-014597-66vd2mdu	943	6	of	of	IN
cord-014597-66vd2mdu	943	7	assays	assay	NNS
cord-014597-66vd2mdu	943	8	is	be	VBZ
cord-014597-66vd2mdu	943	9	shown	show	VBN
cord-014597-66vd2mdu	943	10	here	here	RB
cord-014597-66vd2mdu	943	11	using	use	VBG
cord-014597-66vd2mdu	943	12	the	the	DT
cord-014597-66vd2mdu	943	13	example	example	NN
cord-014597-66vd2mdu	943	14	of	of	IN
cord-014597-66vd2mdu	943	15	CAR	car	NN
cord-014597-66vd2mdu	943	16	-	-	HYPH
cord-014597-66vd2mdu	943	17	T	t	NN
cord-014597-66vd2mdu	943	18	cells	cell	NNS
cord-014597-66vd2mdu	943	19	.	.	.
cord-014597-66vd2mdu	944	1	The	the	DT
cord-014597-66vd2mdu	944	2	image	image	NN
cord-014597-66vd2mdu	944	3	is	be	VBZ
cord-014597-66vd2mdu	944	4	a	a	DT
cord-014597-66vd2mdu	944	5	screenshot	screenshot	NN
cord-014597-66vd2mdu	944	6	from	from	IN
cord-014597-66vd2mdu	944	7	the	the	DT
cord-014597-66vd2mdu	944	8	Genedata	Genedata	NNP
cord-014597-66vd2mdu	944	9	Biologics	Biologics	NNP
cord-014597-66vd2mdu	944	10	™	™	NN
cord-014597-66vd2mdu	944	11	software	software	NN
cord-014597-66vd2mdu	944	12	molecule	molecule	NN
cord-014597-66vd2mdu	944	13	harbors	harbor	VBZ
cord-014597-66vd2mdu	944	14	some	some	DT
cord-014597-66vd2mdu	944	15	stability	stability	NN
cord-014597-66vd2mdu	944	16	issues	issue	NNS
cord-014597-66vd2mdu	944	17	as	as	IN
cord-014597-66vd2mdu	944	18	mentioned	mention	VBN
cord-014597-66vd2mdu	944	19	above	above	RB
cord-014597-66vd2mdu	944	20	.	.	.
cord-014597-66vd2mdu	945	1	Therefore	therefore	RB
cord-014597-66vd2mdu	945	2	,	,	,
cord-014597-66vd2mdu	945	3	more	more	JJR
cord-014597-66vd2mdu	945	4	attempts	attempt	NNS
cord-014597-66vd2mdu	945	5	would	would	MD
cord-014597-66vd2mdu	945	6	be	be	VB
cord-014597-66vd2mdu	945	7	needed	need	VBN
cord-014597-66vd2mdu	945	8	either	either	CC
cord-014597-66vd2mdu	945	9	in	in	IN
cord-014597-66vd2mdu	945	10	formulation	formulation	NN
cord-014597-66vd2mdu	945	11	or	or	CC
cord-014597-66vd2mdu	945	12	in	in	IN
cord-014597-66vd2mdu	945	13	reengineering	reengineering	NN
cord-014597-66vd2mdu	945	14	of	of	IN
cord-014597-66vd2mdu	945	15	the	the	DT
cord-014597-66vd2mdu	945	16	complimentarity	complimentarity	NN
cord-014597-66vd2mdu	945	17	determining	determine	VBG
cord-014597-66vd2mdu	945	18	regions	region	NNS
cord-014597-66vd2mdu	945	19	(	(	-LRB-
cord-014597-66vd2mdu	945	20	CDRs	cdr	NNS
cord-014597-66vd2mdu	945	21	)	)	-RRB-
cord-014597-66vd2mdu	945	22	in	in	IN
cord-014597-66vd2mdu	945	23	order	order	NN
cord-014597-66vd2mdu	945	24	to	to	TO
cord-014597-66vd2mdu	945	25	provide	provide	VB
cord-014597-66vd2mdu	945	26	a	a	DT
cord-014597-66vd2mdu	945	27	developable	developable	JJ
cord-014597-66vd2mdu	945	28	TTP-86-like	TTP-86-like	VBN
cord-014597-66vd2mdu	945	29	drug	drug	NN
cord-014597-66vd2mdu	945	30	candidate	candidate	NN
cord-014597-66vd2mdu	945	31	.	.	.
cord-014597-66vd2mdu	946	1	Background	Background	NNP
cord-014597-66vd2mdu	946	2	Environmental	environmental	JJ
cord-014597-66vd2mdu	946	3	process	process	NN
cord-014597-66vd2mdu	946	4	variables	variable	NNS
cord-014597-66vd2mdu	946	5	are	be	VBP
cord-014597-66vd2mdu	946	6	often	often	RB
cord-014597-66vd2mdu	946	7	used	use	VBN
cord-014597-66vd2mdu	946	8	as	as	IN
cord-014597-66vd2mdu	946	9	tools	tool	NNS
cord-014597-66vd2mdu	946	10	to	to	TO
cord-014597-66vd2mdu	946	11	optimize	optimize	VB
cord-014597-66vd2mdu	946	12	the	the	DT
cord-014597-66vd2mdu	946	13	performance	performance	NN
cord-014597-66vd2mdu	946	14	of	of	IN
cord-014597-66vd2mdu	946	15	mammalian	mammalian	JJ
cord-014597-66vd2mdu	946	16	cell	cell	NN
cord-014597-66vd2mdu	946	17	cultures	culture	NNS
cord-014597-66vd2mdu	946	18	to	to	TO
cord-014597-66vd2mdu	946	19	achieve	achieve	VB
cord-014597-66vd2mdu	946	20	higher	high	JJR
cord-014597-66vd2mdu	946	21	cell	cell	NN
cord-014597-66vd2mdu	946	22	densities	density	NNS
cord-014597-66vd2mdu	946	23	and	and	CC
cord-014597-66vd2mdu	946	24	high	high	JJ
cord-014597-66vd2mdu	946	25	productivities	productivity	NNS
cord-014597-66vd2mdu	946	26	of	of	IN
cord-014597-66vd2mdu	946	27	r	r	NN
cord-014597-66vd2mdu	946	28	-	-	HYPH
cord-014597-66vd2mdu	946	29	proteins	protein	NNS
cord-014597-66vd2mdu	946	30	(	(	-LRB-
cord-014597-66vd2mdu	946	31	q	q	NNP
cord-014597-66vd2mdu	946	32	P	P	NNP
cord-014597-66vd2mdu	946	33	)	)	-RRB-
cord-014597-66vd2mdu	946	34	.	.	.
cord-014597-66vd2mdu	947	1	The	the	DT
cord-014597-66vd2mdu	947	2	manipulation	manipulation	NN
cord-014597-66vd2mdu	947	3	of	of	IN
cord-014597-66vd2mdu	947	4	culture	culture	NN
cord-014597-66vd2mdu	947	5	temperature	temperature	NN
cord-014597-66vd2mdu	947	6	in	in	IN
cord-014597-66vd2mdu	947	7	the	the	DT
cord-014597-66vd2mdu	947	8	range	range	NN
cord-014597-66vd2mdu	947	9	of	of	IN
cord-014597-66vd2mdu	947	10	mild	mild	JJ
cord-014597-66vd2mdu	947	11	hypothermia	hypothermia	NN
cord-014597-66vd2mdu	947	12	(	(	-LRB-
cord-014597-66vd2mdu	947	13	MH	MH	NNP
cord-014597-66vd2mdu	947	14	)	)	-RRB-
cord-014597-66vd2mdu	947	15	(	(	-LRB-
cord-014597-66vd2mdu	947	16	35	35	CD
cord-014597-66vd2mdu	947	17	-	-	SYM
cord-014597-66vd2mdu	947	18	30	30	CD
cord-014597-66vd2mdu	947	19	°	°	NN
cord-014597-66vd2mdu	947	20	C	c	NN
cord-014597-66vd2mdu	947	21	)	)	-RRB-
cord-014597-66vd2mdu	947	22	[	[	-LRB-
cord-014597-66vd2mdu	947	23	1	1	CD
cord-014597-66vd2mdu	947	24	,	,	,
cord-014597-66vd2mdu	947	25	2	2	CD
cord-014597-66vd2mdu	947	26	]	]	-RRB-
cord-014597-66vd2mdu	947	27	,	,	,
cord-014597-66vd2mdu	947	28	as	as	RB
cord-014597-66vd2mdu	947	29	well	well	RB
cord-014597-66vd2mdu	947	30	as	as	IN
cord-014597-66vd2mdu	947	31	different	different	JJ
cord-014597-66vd2mdu	947	32	glucose	glucose	NN
cord-014597-66vd2mdu	947	33	availability	availability	NN
cord-014597-66vd2mdu	947	34	scenarios	scenario	NNS
cord-014597-66vd2mdu	947	35	[	[	-LRB-
cord-014597-66vd2mdu	947	36	3	3	CD
cord-014597-66vd2mdu	947	37	,	,	,
cord-014597-66vd2mdu	947	38	4	4	CD
cord-014597-66vd2mdu	947	39	]	]	-RRB-
cord-014597-66vd2mdu	947	40	,	,	,
cord-014597-66vd2mdu	947	41	has	have	VBZ
cord-014597-66vd2mdu	947	42	been	be	VBN
cord-014597-66vd2mdu	947	43	shown	show	VBN
cord-014597-66vd2mdu	947	44	to	to	TO
cord-014597-66vd2mdu	947	45	improve	improve	VB
cord-014597-66vd2mdu	947	46	productivity	productivity	NN
cord-014597-66vd2mdu	947	47	in	in	IN
cord-014597-66vd2mdu	947	48	different	different	JJ
cord-014597-66vd2mdu	947	49	cell	cell	NN
cord-014597-66vd2mdu	947	50	lines	line	NNS
cord-014597-66vd2mdu	947	51	.	.	.
cord-014597-66vd2mdu	948	1	However	however	RB
cord-014597-66vd2mdu	948	2	,	,	,
cord-014597-66vd2mdu	948	3	the	the	DT
cord-014597-66vd2mdu	948	4	manipulation	manipulation	NN
cord-014597-66vd2mdu	948	5	of	of	IN
cord-014597-66vd2mdu	948	6	these	these	DT
cord-014597-66vd2mdu	948	7	variables	variable	NNS
cord-014597-66vd2mdu	948	8	individually	individually	RB
cord-014597-66vd2mdu	948	9	or	or	CC
cord-014597-66vd2mdu	948	10	together	together	RB
cord-014597-66vd2mdu	948	11	has	have	VBZ
cord-014597-66vd2mdu	948	12	a	a	DT
cord-014597-66vd2mdu	948	13	concomitant	concomitant	JJ
cord-014597-66vd2mdu	948	14	effect	effect	NN
cord-014597-66vd2mdu	948	15	on	on	IN
cord-014597-66vd2mdu	948	16	the	the	DT
cord-014597-66vd2mdu	948	17	rate	rate	NN
cord-014597-66vd2mdu	948	18	at	at	IN
cord-014597-66vd2mdu	948	19	which	which	WDT
cord-014597-66vd2mdu	948	20	cells	cell	NNS
cord-014597-66vd2mdu	948	21	grow	grow	VBP
cord-014597-66vd2mdu	948	22	,	,	,
cord-014597-66vd2mdu	948	23	masking	mask	VBG
cord-014597-66vd2mdu	948	24	the	the	DT
cord-014597-66vd2mdu	948	25	net	net	JJ
cord-014597-66vd2mdu	948	26	response	response	NN
cord-014597-66vd2mdu	948	27	exhibited	exhibit	VBN
cord-014597-66vd2mdu	948	28	by	by	IN
cord-014597-66vd2mdu	948	29	the	the	DT
cord-014597-66vd2mdu	948	30	cells	cell	NNS
cord-014597-66vd2mdu	948	31	.	.	.
cord-014597-66vd2mdu	949	1	In	in	IN
cord-014597-66vd2mdu	949	2	order	order	NN
cord-014597-66vd2mdu	949	3	to	to	TO
cord-014597-66vd2mdu	949	4	identify	identify	VB
cord-014597-66vd2mdu	949	5	the	the	DT
cord-014597-66vd2mdu	949	6	effects	effect	NNS
cord-014597-66vd2mdu	949	7	of	of	IN
cord-014597-66vd2mdu	949	8	these	these	DT
cord-014597-66vd2mdu	949	9	variables	variable	NNS
cord-014597-66vd2mdu	949	10	,	,	,
cord-014597-66vd2mdu	949	11	we	-PRON-	PRP
cord-014597-66vd2mdu	949	12	have	have	VBP
cord-014597-66vd2mdu	949	13	taken	take	VBN
cord-014597-66vd2mdu	949	14	advantage	advantage	NN
cord-014597-66vd2mdu	949	15	of	of	IN
cord-014597-66vd2mdu	949	16	the	the	DT
cord-014597-66vd2mdu	949	17	use	use	NN
cord-014597-66vd2mdu	949	18	of	of	IN
cord-014597-66vd2mdu	949	19	the	the	DT
cord-014597-66vd2mdu	949	20	chemostat	chemostat	NNP
cord-014597-66vd2mdu	949	21	culture	culture	NN
cord-014597-66vd2mdu	949	22	.	.	.
cord-014597-66vd2mdu	950	1	Chemostat	Chemostat	NNP
cord-014597-66vd2mdu	950	2	cultures	culture	NNS
cord-014597-66vd2mdu	950	3	were	be	VBD
cord-014597-66vd2mdu	950	4	performed	perform	VBN
cord-014597-66vd2mdu	950	5	at	at	IN
cord-014597-66vd2mdu	950	6	two	two	CD
cord-014597-66vd2mdu	950	7	dilution	dilution	NN
cord-014597-66vd2mdu	950	8	rate	rate	NN
cord-014597-66vd2mdu	950	9	(	(	-LRB-
cord-014597-66vd2mdu	950	10	D)(0.010	D)(0.010	NNP
cord-014597-66vd2mdu	950	11	or	or	CC
cord-014597-66vd2mdu	950	12	0.018(h-1	0.018(h-1	NN
cord-014597-66vd2mdu	950	13	)	)	-RRB-
cord-014597-66vd2mdu	950	14	)	)	-RRB-
cord-014597-66vd2mdu	950	15	,	,	,
cord-014597-66vd2mdu	950	16	two	two	CD
cord-014597-66vd2mdu	950	17	temperatures	temperature	NNS
cord-014597-66vd2mdu	950	18	(	(	-LRB-
cord-014597-66vd2mdu	950	19	33	33	CD
cord-014597-66vd2mdu	950	20	or	or	CC
cord-014597-66vd2mdu	950	21	37	37	CD
cord-014597-66vd2mdu	950	22	°	°	NN
cord-014597-66vd2mdu	950	23	C	c	NN
cord-014597-66vd2mdu	950	24	)	)	-RRB-
cord-014597-66vd2mdu	950	25	and	and	CC
cord-014597-66vd2mdu	950	26	three	three	CD
cord-014597-66vd2mdu	950	27	feed	feed	NN
cord-014597-66vd2mdu	950	28	glucose	glucose	NN
cord-014597-66vd2mdu	950	29	concentrations	concentration	NNS
cord-014597-66vd2mdu	950	30	(	(	-LRB-
cord-014597-66vd2mdu	950	31	20	20	CD
cord-014597-66vd2mdu	950	32	,	,	,
cord-014597-66vd2mdu	950	33	30	30	CD
cord-014597-66vd2mdu	950	34	or	or	CC
cord-014597-66vd2mdu	950	35	40	40	CD
cord-014597-66vd2mdu	950	36	mM	mm	CD
cord-014597-66vd2mdu	950	37	)	)	-RRB-
cord-014597-66vd2mdu	950	38	.	.	.
cord-014597-66vd2mdu	951	1	The	the	DT
cord-014597-66vd2mdu	951	2	response	response	NN
cord-014597-66vd2mdu	951	3	was	be	VBD
cord-014597-66vd2mdu	951	4	analysed	analyse	VBN
cord-014597-66vd2mdu	951	5	considering	consider	VBG
cord-014597-66vd2mdu	951	6	r	r	NN
cord-014597-66vd2mdu	951	7	-	-	HYPH
cord-014597-66vd2mdu	951	8	protein	protein	NN
cord-014597-66vd2mdu	951	9	production	production	NN
cord-014597-66vd2mdu	951	10	,	,	,
cord-014597-66vd2mdu	951	11	cell	cell	NN
cord-014597-66vd2mdu	951	12	growth	growth	NN
cord-014597-66vd2mdu	951	13	and	and	CC
cord-014597-66vd2mdu	951	14	key	key	JJ
cord-014597-66vd2mdu	951	15	metabolites	metabolite	NNS
cord-014597-66vd2mdu	951	16	.	.	.
cord-014597-66vd2mdu	952	1	r	r	LS
cord-014597-66vd2mdu	952	2	-	-	:
cord-014597-66vd2mdu	952	3	tPA	tpa	NN
cord-014597-66vd2mdu	952	4	protein	protein	NN
cord-014597-66vd2mdu	952	5	concentration	concentration	NN
cord-014597-66vd2mdu	952	6	was	be	VBD
cord-014597-66vd2mdu	952	7	determined	determine	VBN
cord-014597-66vd2mdu	952	8	by	by	IN
cord-014597-66vd2mdu	952	9	immunoassay	immunoassay	JJ
cord-014597-66vd2mdu	952	10	(	(	-LRB-
cord-014597-66vd2mdu	952	11	TriniLIZE	TriniLIZE	NNP
cord-014597-66vd2mdu	952	12	tPA	tPA	NNP
cord-014597-66vd2mdu	952	13	KIT	kit	NN
cord-014597-66vd2mdu	952	14	)	)	-RRB-
cord-014597-66vd2mdu	952	15	;	;	:
cord-014597-66vd2mdu	952	16	cells	cell	NNS
cord-014597-66vd2mdu	952	17	were	be	VBD
cord-014597-66vd2mdu	952	18	counted	count	VBN
cord-014597-66vd2mdu	952	19	using	use	VBG
cord-014597-66vd2mdu	952	20	a	a	DT
cord-014597-66vd2mdu	952	21	hemocytometer	hemocytometer	NN
cord-014597-66vd2mdu	952	22	and	and	CC
cord-014597-66vd2mdu	952	23	cell	cell	NN
cord-014597-66vd2mdu	952	24	viability	viability	NN
cord-014597-66vd2mdu	952	25	was	be	VBD
cord-014597-66vd2mdu	952	26	determined	determine	VBN
cord-014597-66vd2mdu	952	27	by	by	IN
cord-014597-66vd2mdu	952	28	the	the	DT
cord-014597-66vd2mdu	952	29	method	method	NN
cord-014597-66vd2mdu	952	30	of	of	IN
cord-014597-66vd2mdu	952	31	exclusion	exclusion	NN
cord-014597-66vd2mdu	952	32	using	use	VBG
cord-014597-66vd2mdu	952	33	trypan	trypan	NNP
cord-014597-66vd2mdu	952	34	blue	blue	NNP
cord-014597-66vd2mdu	952	35	(	(	-LRB-
cord-014597-66vd2mdu	952	36	T8154	T8154	NNP
cord-014597-66vd2mdu	952	37	,	,	,
cord-014597-66vd2mdu	952	38	Sigma	Sigma	NNP
cord-014597-66vd2mdu	952	39	,	,	,
cord-014597-66vd2mdu	952	40	USA	USA	NNP
cord-014597-66vd2mdu	952	41	)	)	-RRB-
cord-014597-66vd2mdu	952	42	;	;	:
cord-014597-66vd2mdu	952	43	glucose	glucose	NN
cord-014597-66vd2mdu	952	44	,	,	,
cord-014597-66vd2mdu	952	45	lactate	lactate	NN
cord-014597-66vd2mdu	952	46	and	and	CC
cord-014597-66vd2mdu	952	47	glutamate	glutamate	NN
cord-014597-66vd2mdu	952	48	were	be	VBD
cord-014597-66vd2mdu	952	49	determined	determine	VBN
cord-014597-66vd2mdu	952	50	by	by	IN
cord-014597-66vd2mdu	952	51	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	952	52	assay	assay	NN
cord-014597-66vd2mdu	952	53	using	use	VBG
cord-014597-66vd2mdu	952	54	a	a	DT
cord-014597-66vd2mdu	952	55	biochemical	biochemical	JJ
cord-014597-66vd2mdu	952	56	analyser	analyser	NN
cord-014597-66vd2mdu	952	57	YSI	YSI	NNP
cord-014597-66vd2mdu	952	58	(	(	-LRB-
cord-014597-66vd2mdu	952	59	Yellow	Yellow	NNP
cord-014597-66vd2mdu	952	60	Spring	Spring	NNP
cord-014597-66vd2mdu	952	61	Instruments	Instruments	NNP
cord-014597-66vd2mdu	952	62	)	)	-RRB-
cord-014597-66vd2mdu	952	63	.	.	.
cord-014597-66vd2mdu	953	1	Statistical	statistical	JJ
cord-014597-66vd2mdu	953	2	analysis	analysis	NN
cord-014597-66vd2mdu	953	3	of	of	IN
cord-014597-66vd2mdu	953	4	the	the	DT
cord-014597-66vd2mdu	953	5	results	result	NNS
cord-014597-66vd2mdu	953	6	was	be	VBD
cord-014597-66vd2mdu	953	7	performed	perform	VBN
cord-014597-66vd2mdu	953	8	by	by	IN
cord-014597-66vd2mdu	953	9	ANOVA	ANOVA	NNP
cord-014597-66vd2mdu	953	10	(	(	-LRB-
cord-014597-66vd2mdu	953	11	Design	Design	NNP
cord-014597-66vd2mdu	953	12	-	-	HYPH
cord-014597-66vd2mdu	953	13	Expert	Expert	NNP
cord-014597-66vd2mdu	953	14	7	7	CD
cord-014597-66vd2mdu	953	15	for	for	IN
cord-014597-66vd2mdu	953	16	Windows	Windows	NNP
cord-014597-66vd2mdu	953	17	)	)	-RRB-
cord-014597-66vd2mdu	953	18	.	.	.
cord-014597-66vd2mdu	954	1	A	a	DT
cord-014597-66vd2mdu	954	2	decrease	decrease	NN
cord-014597-66vd2mdu	954	3	in	in	IN
cord-014597-66vd2mdu	954	4	cell	cell	NN
cord-014597-66vd2mdu	954	5	density	density	NN
cord-014597-66vd2mdu	954	6	was	be	VBD
cord-014597-66vd2mdu	954	7	observed	observe	VBN
cord-014597-66vd2mdu	954	8	in	in	IN
cord-014597-66vd2mdu	954	9	response	response	NN
cord-014597-66vd2mdu	954	10	to	to	IN
cord-014597-66vd2mdu	954	11	an	an	DT
cord-014597-66vd2mdu	954	12	increase	increase	NN
cord-014597-66vd2mdu	954	13	of	of	IN
cord-014597-66vd2mdu	954	14	glucose	glucose	NN
cord-014597-66vd2mdu	954	15	feeding	feeding	NN
cord-014597-66vd2mdu	954	16	concentration	concentration	NN
cord-014597-66vd2mdu	954	17	,	,	,
cord-014597-66vd2mdu	954	18	regardless	regardless	RB
cord-014597-66vd2mdu	954	19	of	of	IN
cord-014597-66vd2mdu	954	20	temperature	temperature	NN
cord-014597-66vd2mdu	954	21	or	or	CC
cord-014597-66vd2mdu	954	22	specific	specific	JJ
cord-014597-66vd2mdu	954	23	growth	growth	NN
cord-014597-66vd2mdu	954	24	rate	rate	NN
cord-014597-66vd2mdu	954	25	(	(	-LRB-
cord-014597-66vd2mdu	954	26	in	in	IN
cord-014597-66vd2mdu	954	27	this	this	DT
cord-014597-66vd2mdu	954	28	case	case	NN
cord-014597-66vd2mdu	954	29	μ	μ	NN
cord-014597-66vd2mdu	954	30	=	=	SYM
cord-014597-66vd2mdu	954	31	D	D	NNP
cord-014597-66vd2mdu	954	32	)	)	-RRB-
cord-014597-66vd2mdu	954	33	evaluated	evaluate	VBN
cord-014597-66vd2mdu	954	34	.	.	.
cord-014597-66vd2mdu	955	1	The	the	DT
cord-014597-66vd2mdu	955	2	maximum	maximum	JJ
cord-014597-66vd2mdu	955	3	cell	cell	NN
cord-014597-66vd2mdu	955	4	densities	density	NNS
cord-014597-66vd2mdu	955	5	were	be	VBD
cord-014597-66vd2mdu	955	6	reached	reach	VBN
cord-014597-66vd2mdu	955	7	at	at	IN
cord-014597-66vd2mdu	955	8	20mM	20mm	CD
cord-014597-66vd2mdu	955	9	,	,	,
cord-014597-66vd2mdu	955	10	achieving	achieve	VBG
cord-014597-66vd2mdu	955	11	1.65	1.65	CD
cord-014597-66vd2mdu	955	12	and	and	CC
cord-014597-66vd2mdu	955	13	1.50	1.50	CD
cord-014597-66vd2mdu	955	14	x10	x10	CD
cord-014597-66vd2mdu	955	15	6	6	CD
cord-014597-66vd2mdu	955	16	cells	cell	NNS
cord-014597-66vd2mdu	956	1	/ml	/ml	NFP
cord-014597-66vd2mdu	956	2	at	at	IN
cord-014597-66vd2mdu	956	3	37/33	37/33	CD
cord-014597-66vd2mdu	956	4	°	°	NN
cord-014597-66vd2mdu	956	5	C	c	NN
cord-014597-66vd2mdu	956	6	and	and	CC
cord-014597-66vd2mdu	956	7	0.018(h	0.018(h	CD
cord-014597-66vd2mdu	956	8	-1	-1	NN
cord-014597-66vd2mdu	956	9	)	)	-RRB-
cord-014597-66vd2mdu	956	10	;	;	:
cord-014597-66vd2mdu	956	11	and	and	CC
cord-014597-66vd2mdu	956	12	1.10	1.10	CD
cord-014597-66vd2mdu	956	13	and	and	CC
cord-014597-66vd2mdu	956	14	1.33	1.33	CD
cord-014597-66vd2mdu	956	15	x	x	SYM
cord-014597-66vd2mdu	956	16	10	10	CD
cord-014597-66vd2mdu	956	17	6	6	CD
cord-014597-66vd2mdu	956	18	cells	cell	NNS
cord-014597-66vd2mdu	956	19	/	/	SYM
cord-014597-66vd2mdu	956	20	ml	ml	NN
cord-014597-66vd2mdu	956	21	at	at	IN
cord-014597-66vd2mdu	956	22	37/33	37/33	CD
cord-014597-66vd2mdu	956	23	°	°	NN
cord-014597-66vd2mdu	956	24	C	c	NN
cord-014597-66vd2mdu	956	25	and	and	CC
cord-014597-66vd2mdu	956	26	0.010(h	0.010(h	CD
cord-014597-66vd2mdu	956	27	-1	-1	NN
cord-014597-66vd2mdu	956	28	)	)	-RRB-
cord-014597-66vd2mdu	957	1	respectively	respectively	RB
cord-014597-66vd2mdu	957	2	(	(	-LRB-
cord-014597-66vd2mdu	957	3	Fig	Fig	NNP
cord-014597-66vd2mdu	957	4	.	.	.
cord-014597-66vd2mdu	957	5	1a	1a	CD
cord-014597-66vd2mdu	957	6	)	)	-RRB-
cord-014597-66vd2mdu	957	7	.	.	.
cord-014597-66vd2mdu	958	1	The	the	DT
cord-014597-66vd2mdu	958	2	increase	increase	NN
cord-014597-66vd2mdu	958	3	in	in	IN
cord-014597-66vd2mdu	958	4	glucose	glucose	NN
cord-014597-66vd2mdu	958	5	concentration	concentration	NN
cord-014597-66vd2mdu	958	6	from	from	IN
cord-014597-66vd2mdu	958	7	20	20	CD
cord-014597-66vd2mdu	958	8	to	to	TO
cord-014597-66vd2mdu	958	9	40mM	40mm	CD
cord-014597-66vd2mdu	958	10	resulted	result	VBD
cord-014597-66vd2mdu	958	11	in	in	IN
cord-014597-66vd2mdu	958	12	an	an	DT
cord-014597-66vd2mdu	958	13	q	q	NN
cord-014597-66vd2mdu	958	14	P	p	NN
cord-014597-66vd2mdu	958	15	increase	increase	NN
cord-014597-66vd2mdu	958	16	of	of	IN
cord-014597-66vd2mdu	958	17	3	3	CD
cord-014597-66vd2mdu	958	18	and	and	CC
cord-014597-66vd2mdu	958	19	3.3	3.3	CD
cord-014597-66vd2mdu	958	20	fold	fold	VB
cord-014597-66vd2mdu	958	21	at	at	IN
cord-014597-66vd2mdu	958	22	33	33	CD
cord-014597-66vd2mdu	958	23	°	°	NN
cord-014597-66vd2mdu	958	24	C/0.018(h	C/0.018(h	NNP
cord-014597-66vd2mdu	958	25	-1	-1	NN
cord-014597-66vd2mdu	958	26	)	)	-RRB-
cord-014597-66vd2mdu	958	27	and	and	CC
cord-014597-66vd2mdu	958	28	37	37	CD
cord-014597-66vd2mdu	958	29	°	°	NN
cord-014597-66vd2mdu	958	30	C/0.018(h	C/0.018(h	NNP
cord-014597-66vd2mdu	958	31	-1	-1	NN
cord-014597-66vd2mdu	958	32	)	)	-RRB-
cord-014597-66vd2mdu	958	33	respectively	respectively	RB
cord-014597-66vd2mdu	958	34	.	.	.
cord-014597-66vd2mdu	959	1	A	a	DT
cord-014597-66vd2mdu	959	2	lower	low	JJR
cord-014597-66vd2mdu	959	3	increase	increase	NN
cord-014597-66vd2mdu	959	4	of	of	IN
cord-014597-66vd2mdu	959	5	2.4	2.4	CD
cord-014597-66vd2mdu	959	6	and	and	CC
cord-014597-66vd2mdu	959	7	1.8	1.8	CD
cord-014597-66vd2mdu	959	8	fold	fold	NN
cord-014597-66vd2mdu	959	9	was	be	VBD
cord-014597-66vd2mdu	959	10	reached	reach	VBN
cord-014597-66vd2mdu	959	11	at	at	IN
cord-014597-66vd2mdu	959	12	33	33	CD
cord-014597-66vd2mdu	959	13	°	°	NN
cord-014597-66vd2mdu	959	14	C/0.010(h	C/0.010(h	NNP
cord-014597-66vd2mdu	959	15	-1	-1	NNP
cord-014597-66vd2mdu	959	16	)	)	-RRB-
cord-014597-66vd2mdu	959	17	and	and	CC
cord-014597-66vd2mdu	959	18	37	37	CD
cord-014597-66vd2mdu	959	19	°	°	NN
cord-014597-66vd2mdu	959	20	C/0.010(h	C/0.010(h	NNP
cord-014597-66vd2mdu	959	21	-1	-1	NN
cord-014597-66vd2mdu	959	22	)	)	-RRB-
cord-014597-66vd2mdu	959	23	respectively	respectively	RB
cord-014597-66vd2mdu	960	1	(	(	-LRB-
cord-014597-66vd2mdu	960	2	Fig	Fig	NNP
cord-014597-66vd2mdu	960	3	.	.	.
cord-014597-66vd2mdu	960	4	1b	1b	CD
cord-014597-66vd2mdu	960	5	)	)	-RRB-
cord-014597-66vd2mdu	960	6	.	.	.
cord-014597-66vd2mdu	961	1	The	the	DT
cord-014597-66vd2mdu	961	2	highest	high	JJS
cord-014597-66vd2mdu	961	3	q	q	NN
cord-014597-66vd2mdu	961	4	P	P	NNP
cord-014597-66vd2mdu	961	5	s	s	NNS
cord-014597-66vd2mdu	961	6	were	be	VBD
cord-014597-66vd2mdu	961	7	reached	reach	VBN
cord-014597-66vd2mdu	961	8	at	at	IN
cord-014597-66vd2mdu	961	9	37	37	CD
cord-014597-66vd2mdu	961	10	°	°	NN
cord-014597-66vd2mdu	961	11	C	c	NN
cord-014597-66vd2mdu	961	12	and	and	CC
cord-014597-66vd2mdu	961	13	0.010(h	0.010(h	CD
cord-014597-66vd2mdu	961	14	-1	-1	NN
cord-014597-66vd2mdu	961	15	)	)	-RRB-
cord-014597-66vd2mdu	961	16	.	.	.
cord-014597-66vd2mdu	962	1	However	however	RB
cord-014597-66vd2mdu	962	2	,	,	,
cord-014597-66vd2mdu	962	3	a	a	DT
cord-014597-66vd2mdu	962	4	positive	positive	JJ
cord-014597-66vd2mdu	962	5	effect	effect	NN
cord-014597-66vd2mdu	962	6	of	of	IN
cord-014597-66vd2mdu	962	7	MH	MH	NNP
cord-014597-66vd2mdu	962	8	was	be	VBD
cord-014597-66vd2mdu	962	9	not	not	RB
cord-014597-66vd2mdu	962	10	observed	observe	VBN
cord-014597-66vd2mdu	962	11	,	,	,
cord-014597-66vd2mdu	962	12	in	in	IN
cord-014597-66vd2mdu	962	13	contrast	contrast	NN
cord-014597-66vd2mdu	962	14	to	to	IN
cord-014597-66vd2mdu	962	15	that	that	DT
cord-014597-66vd2mdu	962	16	observed	observe	VBN
cord-014597-66vd2mdu	962	17	in	in	IN
cord-014597-66vd2mdu	962	18	batch	batch	NN
cord-014597-66vd2mdu	962	19	culture	culture	NN
cord-014597-66vd2mdu	962	20	[	[	-LRB-
cord-014597-66vd2mdu	962	21	1	1	CD
cord-014597-66vd2mdu	962	22	,	,	,
cord-014597-66vd2mdu	962	23	2	2	CD
cord-014597-66vd2mdu	962	24	,	,	,
cord-014597-66vd2mdu	962	25	3	3	CD
cord-014597-66vd2mdu	962	26	]	]	-RRB-
cord-014597-66vd2mdu	962	27	.	.	.
cord-014597-66vd2mdu	963	1	This	this	DT
cord-014597-66vd2mdu	963	2	behaviour	behaviour	NN
cord-014597-66vd2mdu	963	3	suggests	suggest	VBZ
cord-014597-66vd2mdu	963	4	that	that	IN
cord-014597-66vd2mdu	963	5	low	low	JJ
cord-014597-66vd2mdu	963	6	μ	μ	NN
cord-014597-66vd2mdu	963	7	is	be	VBZ
cord-014597-66vd2mdu	963	8	a	a	DT
cord-014597-66vd2mdu	963	9	main	main	JJ
cord-014597-66vd2mdu	963	10	factor	factor	NN
cord-014597-66vd2mdu	963	11	on	on	IN
cord-014597-66vd2mdu	963	12	increased	increase	VBN
cord-014597-66vd2mdu	963	13	r	r	NN
cord-014597-66vd2mdu	963	14	-	-	HYPH
cord-014597-66vd2mdu	963	15	protein	protein	NN
cord-014597-66vd2mdu	963	16	production	production	NN
cord-014597-66vd2mdu	963	17	in	in	IN
cord-014597-66vd2mdu	963	18	batch	batch	NN
cord-014597-66vd2mdu	963	19	cultures	culture	NNS
cord-014597-66vd2mdu	963	20	exposed	expose	VBN
cord-014597-66vd2mdu	963	21	at	at	IN
cord-014597-66vd2mdu	963	22	MH	MH	NNP
cord-014597-66vd2mdu	963	23	condition	condition	NN
cord-014597-66vd2mdu	963	24	.	.	.
cord-014597-66vd2mdu	964	1	The	the	DT
cord-014597-66vd2mdu	964	2	specific	specific	JJ
cord-014597-66vd2mdu	964	3	consumption	consumption	NN
cord-014597-66vd2mdu	964	4	rate	rate	NN
cord-014597-66vd2mdu	964	5	of	of	IN
cord-014597-66vd2mdu	964	6	glucose	glucose	NN
cord-014597-66vd2mdu	964	7	was	be	VBD
cord-014597-66vd2mdu	964	8	significantly	significantly	RB
cord-014597-66vd2mdu	964	9	increased	increase	VBN
cord-014597-66vd2mdu	964	10	by	by	IN
cord-014597-66vd2mdu	964	11	the	the	DT
cord-014597-66vd2mdu	964	12	glucose	glucose	NN
cord-014597-66vd2mdu	964	13	increase	increase	NN
cord-014597-66vd2mdu	964	14	from	from	IN
cord-014597-66vd2mdu	964	15	20	20	CD
cord-014597-66vd2mdu	964	16	to	to	TO
cord-014597-66vd2mdu	964	17	40mM	40mm	CD
cord-014597-66vd2mdu	964	18	and	and	CC
cord-014597-66vd2mdu	964	19	reduced	reduce	VBN
cord-014597-66vd2mdu	964	20	by	by	IN
cord-014597-66vd2mdu	964	21	MH	MH	NNP
cord-014597-66vd2mdu	964	22	(	(	-LRB-
cord-014597-66vd2mdu	964	23	Fig	Fig	NNP
cord-014597-66vd2mdu	964	24	.	.	.
cord-014597-66vd2mdu	964	25	 	 	_SP
cord-014597-66vd2mdu	964	26	1c	1c	CD
cord-014597-66vd2mdu	964	27	)	)	-RRB-
cord-014597-66vd2mdu	964	28	.	.	.
cord-014597-66vd2mdu	965	1	At	at	IN
cord-014597-66vd2mdu	965	2	0.010	0.010	CD
cord-014597-66vd2mdu	965	3	(	(	-LRB-
cord-014597-66vd2mdu	965	4	h	h	NN
cord-014597-66vd2mdu	965	5	-1	-1	JJ
cord-014597-66vd2mdu	965	6	)	)	-RRB-
cord-014597-66vd2mdu	965	7	the	the	DT
cord-014597-66vd2mdu	965	8	specific	specific	JJ
cord-014597-66vd2mdu	965	9	production	production	NN
cord-014597-66vd2mdu	965	10	rate	rate	NN
cord-014597-66vd2mdu	965	11	of	of	IN
cord-014597-66vd2mdu	965	12	lactate	lactate	NN
cord-014597-66vd2mdu	965	13	(	(	-LRB-
cord-014597-66vd2mdu	965	14	q	q	NNP
cord-014597-66vd2mdu	965	15	Lac	Lac	NNP
cord-014597-66vd2mdu	965	16	)	)	-RRB-
cord-014597-66vd2mdu	965	17	was	be	VBD
cord-014597-66vd2mdu	965	18	increased	increase	VBN
cord-014597-66vd2mdu	965	19	by	by	IN
cord-014597-66vd2mdu	965	20	glucose	glucose	NN
cord-014597-66vd2mdu	965	21	increase	increase	NN
cord-014597-66vd2mdu	965	22	,	,	,
cord-014597-66vd2mdu	965	23	independent	independent	JJ
cord-014597-66vd2mdu	965	24	of	of	IN
cord-014597-66vd2mdu	965	25	the	the	DT
cord-014597-66vd2mdu	965	26	culture	culture	NN
cord-014597-66vd2mdu	965	27	temperature	temperature	NN
cord-014597-66vd2mdu	965	28	used	use	VBN
cord-014597-66vd2mdu	965	29	.	.	.
cord-014597-66vd2mdu	966	1	While	while	IN
cord-014597-66vd2mdu	966	2	at	at	IN
cord-014597-66vd2mdu	966	3	33	33	CD
cord-014597-66vd2mdu	966	4	°	°	NN
cord-014597-66vd2mdu	966	5	C/0.018(h	C/0.018(h	NNP
cord-014597-66vd2mdu	966	6	-1	-1	NN
cord-014597-66vd2mdu	966	7	)	)	-RRB-
cord-014597-66vd2mdu	967	1	the	the	DT
cord-014597-66vd2mdu	967	2	q	q	NNP
cord-014597-66vd2mdu	967	3	Lac	Lac	NNP
cord-014597-66vd2mdu	967	4	decreased	decrease	VBD
cord-014597-66vd2mdu	967	5	with	with	IN
cord-014597-66vd2mdu	967	6	increasing	increase	VBG
cord-014597-66vd2mdu	967	7	glucose	glucose	NN
cord-014597-66vd2mdu	967	8	concentration	concentration	NN
cord-014597-66vd2mdu	967	9	and	and	CC
cord-014597-66vd2mdu	967	10	at	at	IN
cord-014597-66vd2mdu	967	11	37	37	CD
cord-014597-66vd2mdu	967	12	°	°	NN
cord-014597-66vd2mdu	967	13	C/0.018(h	C/0.018(h	NNP
cord-014597-66vd2mdu	967	14	-1	-1	NN
cord-014597-66vd2mdu	967	15	)	)	-RRB-
cord-014597-66vd2mdu	968	1	a	a	DT
cord-014597-66vd2mdu	968	2	maximum	maximum	JJ
cord-014597-66vd2mdu	968	3	consumption	consumption	NN
cord-014597-66vd2mdu	968	4	was	be	VBD
cord-014597-66vd2mdu	968	5	observed	observe	VBN
cord-014597-66vd2mdu	968	6	at	at	IN
cord-014597-66vd2mdu	968	7	30	30	CD
cord-014597-66vd2mdu	968	8	mM	mm	CD
cord-014597-66vd2mdu	968	9	glucose	glucose	NN
cord-014597-66vd2mdu	968	10	(	(	-LRB-
cord-014597-66vd2mdu	968	11	Fig	Fig	NNP
cord-014597-66vd2mdu	968	12	.	.	.
cord-014597-66vd2mdu	968	13	1d	1d	CD
cord-014597-66vd2mdu	968	14	)	)	-RRB-
cord-014597-66vd2mdu	968	15	.	.	.
cord-014597-66vd2mdu	969	1	The	the	DT
cord-014597-66vd2mdu	969	2	lactate	lactate	NN
cord-014597-66vd2mdu	969	3	-	-	HYPH
cord-014597-66vd2mdu	969	4	glucose	glucose	NN
cord-014597-66vd2mdu	969	5	yield	yield	NN
cord-014597-66vd2mdu	969	6	(	(	-LRB-
cord-014597-66vd2mdu	969	7	Fig	Fig	NNP
cord-014597-66vd2mdu	969	8	.	.	.
cord-014597-66vd2mdu	969	9	1e	1e	LS
cord-014597-66vd2mdu	969	10	)	)	-RRB-
cord-014597-66vd2mdu	969	11	not	not	RB
cord-014597-66vd2mdu	969	12	showed	show	VBD
cord-014597-66vd2mdu	969	13	relevant	relevant	JJ
cord-014597-66vd2mdu	969	14	changes	change	NNS
cord-014597-66vd2mdu	969	15	at	at	IN
cord-014597-66vd2mdu	969	16	0.010(h	0.010(h	CD
cord-014597-66vd2mdu	969	17	-1	-1	CD
cord-014597-66vd2mdu	969	18	)	)	-RRB-
cord-014597-66vd2mdu	969	19	,	,	,
cord-014597-66vd2mdu	969	20	while	while	IN
cord-014597-66vd2mdu	969	21	at	at	IN
cord-014597-66vd2mdu	969	22	0.018(h	0.018(h	CD
cord-014597-66vd2mdu	969	23	-1	-1	NN
cord-014597-66vd2mdu	969	24	)	)	-RRB-
cord-014597-66vd2mdu	970	1	this	this	DT
cord-014597-66vd2mdu	970	2	yield	yield	NN
cord-014597-66vd2mdu	970	3	showed	show	VBD
cord-014597-66vd2mdu	970	4	a	a	DT
cord-014597-66vd2mdu	970	5	more	more	RBR
cord-014597-66vd2mdu	970	6	efficient	efficient	JJ
cord-014597-66vd2mdu	970	7	utilization	utilization	NN
cord-014597-66vd2mdu	970	8	of	of	IN
cord-014597-66vd2mdu	970	9	glucose	glucose	NN
cord-014597-66vd2mdu	970	10	,	,	,
cord-014597-66vd2mdu	970	11	as	as	IN
cord-014597-66vd2mdu	970	12	glucose	glucose	NN
cord-014597-66vd2mdu	970	13	concentration	concentration	NN
cord-014597-66vd2mdu	970	14	was	be	VBD
cord-014597-66vd2mdu	970	15	increased	increase	VBN
cord-014597-66vd2mdu	970	16	.	.	.
cord-014597-66vd2mdu	971	1	However	however	RB
cord-014597-66vd2mdu	971	2	,	,	,
cord-014597-66vd2mdu	971	3	this	this	DT
cord-014597-66vd2mdu	971	4	last	last	JJ
cord-014597-66vd2mdu	971	5	behaviour	behaviour	NN
cord-014597-66vd2mdu	971	6	was	be	VBD
cord-014597-66vd2mdu	971	7	not	not	RB
cord-014597-66vd2mdu	971	8	reflected	reflect	VBN
cord-014597-66vd2mdu	971	9	in	in	IN
cord-014597-66vd2mdu	971	10	an	an	DT
cord-014597-66vd2mdu	971	11	increase	increase	NN
cord-014597-66vd2mdu	971	12	of	of	IN
cord-014597-66vd2mdu	971	13	r	r	NN
cord-014597-66vd2mdu	971	14	-	-	HYPH
cord-014597-66vd2mdu	971	15	protein	protein	NN
cord-014597-66vd2mdu	971	16	production	production	NN
cord-014597-66vd2mdu	971	17	.	.	.
cord-014597-66vd2mdu	972	1	The	the	DT
cord-014597-66vd2mdu	972	2	concentration	concentration	NN
cord-014597-66vd2mdu	972	3	of	of	IN
cord-014597-66vd2mdu	972	4	glucose	glucose	NN
cord-014597-66vd2mdu	972	5	has	have	VBZ
cord-014597-66vd2mdu	972	6	the	the	DT
cord-014597-66vd2mdu	972	7	greatest	great	JJS
cord-014597-66vd2mdu	972	8	impact	impact	NN
cord-014597-66vd2mdu	972	9	on	on	IN
cord-014597-66vd2mdu	972	10	the	the	DT
cord-014597-66vd2mdu	972	11	behaviour	behaviour	NN
cord-014597-66vd2mdu	972	12	of	of	IN
cord-014597-66vd2mdu	972	13	the	the	DT
cord-014597-66vd2mdu	972	14	culture	culture	NN
cord-014597-66vd2mdu	972	15	,	,	,
cord-014597-66vd2mdu	972	16	and	and	CC
cord-014597-66vd2mdu	972	17	its	-PRON-	PRP$
cord-014597-66vd2mdu	972	18	increase	increase	NN
cord-014597-66vd2mdu	972	19	affects	affect	VBZ
cord-014597-66vd2mdu	972	20	positively	positively	RB
cord-014597-66vd2mdu	972	21	the	the	DT
cord-014597-66vd2mdu	972	22	protein	protein	NN
cord-014597-66vd2mdu	972	23	productivity	productivity	NN
cord-014597-66vd2mdu	972	24	.	.	.
cord-014597-66vd2mdu	973	1	The	the	DT
cord-014597-66vd2mdu	973	2	MH	MH	NNP
cord-014597-66vd2mdu	973	3	did	do	VBD
cord-014597-66vd2mdu	973	4	not	not	RB
cord-014597-66vd2mdu	973	5	improve	improve	VB
cord-014597-66vd2mdu	973	6	proteins	protein	NNS
cord-014597-66vd2mdu	973	7	productivity	productivity	NN
cord-014597-66vd2mdu	973	8	of	of	IN
cord-014597-66vd2mdu	973	9	CHO	CHO	NNP
cord-014597-66vd2mdu	973	10	cells	cell	NNS
cord-014597-66vd2mdu	973	11	producing	produce	VBG
cord-014597-66vd2mdu	973	12	tPA	tPA	NNP
cord-014597-66vd2mdu	973	13	under	under	IN
cord-014597-66vd2mdu	973	14	the	the	DT
cord-014597-66vd2mdu	973	15	different	different	JJ
cord-014597-66vd2mdu	973	16	conditions	condition	NNS
cord-014597-66vd2mdu	973	17	evaluated	evaluate	VBN
cord-014597-66vd2mdu	973	18	;	;	:
cord-014597-66vd2mdu	973	19	low	low	JJ
cord-014597-66vd2mdu	973	20	dilution	dilution	NN
cord-014597-66vd2mdu	973	21	rate	rate	NN
cord-014597-66vd2mdu	973	22	and	and	CC
cord-014597-66vd2mdu	973	23	at	at	IN
cord-014597-66vd2mdu	973	24	high	high	JJ
cord-014597-66vd2mdu	973	25	glucose	glucose	NN
cord-014597-66vd2mdu	973	26	concentration	concentration	NN
cord-014597-66vd2mdu	973	27	impact	impact	NN
cord-014597-66vd2mdu	973	28	positively	positively	RB
cord-014597-66vd2mdu	973	29	the	the	DT
cord-014597-66vd2mdu	973	30	protein	protein	NN
cord-014597-66vd2mdu	973	31	productivity	productivity	NN
cord-014597-66vd2mdu	973	32	and	and	CC
cord-014597-66vd2mdu	973	33	the	the	DT
cord-014597-66vd2mdu	973	34	metabolism	metabolism	NN
cord-014597-66vd2mdu	973	35	exhibited	exhibit	VBN
cord-014597-66vd2mdu	973	36	by	by	IN
cord-014597-66vd2mdu	973	37	the	the	DT
cord-014597-66vd2mdu	973	38	cells	cell	NNS
cord-014597-66vd2mdu	973	39	.	.	.
cord-014597-66vd2mdu	974	1	Background	background	NN
cord-014597-66vd2mdu	974	2	Mammalian	mammalian	JJ
cord-014597-66vd2mdu	974	3	cell	cell	NN
cord-014597-66vd2mdu	974	4	cultures	culture	NNS
cord-014597-66vd2mdu	974	5	are	be	VBP
cord-014597-66vd2mdu	974	6	the	the	DT
cord-014597-66vd2mdu	974	7	most	most	RBS
cord-014597-66vd2mdu	974	8	commonly	commonly	RB
cord-014597-66vd2mdu	974	9	used	use	VBN
cord-014597-66vd2mdu	974	10	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	974	11	for	for	IN
cord-014597-66vd2mdu	974	12	the	the	DT
cord-014597-66vd2mdu	974	13	production	production	NN
cord-014597-66vd2mdu	974	14	of	of	IN
cord-014597-66vd2mdu	974	15	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	974	16	recombinant	recombinant	JJ
cord-014597-66vd2mdu	974	17	proteins	protein	NNS
cord-014597-66vd2mdu	974	18	such	such	JJ
cord-014597-66vd2mdu	974	19	as	as	IN
cord-014597-66vd2mdu	974	20	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	974	21	antibodies	antibody	NNS
cord-014597-66vd2mdu	974	22	(	(	-LRB-
cord-014597-66vd2mdu	974	23	mAbs	mAbs	NNP
cord-014597-66vd2mdu	974	24	)	)	-RRB-
cord-014597-66vd2mdu	974	25	.	.	.
cord-014597-66vd2mdu	975	1	Facing	face	VBG
cord-014597-66vd2mdu	975	2	to	to	IN
cord-014597-66vd2mdu	975	3	the	the	DT
cord-014597-66vd2mdu	975	4	increasing	increase	VBG
cord-014597-66vd2mdu	975	5	demand	demand	NN
cord-014597-66vd2mdu	975	6	of	of	IN
cord-014597-66vd2mdu	975	7	these	these	DT
cord-014597-66vd2mdu	975	8	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	975	9	,	,	,
cord-014597-66vd2mdu	975	10	the	the	DT
cord-014597-66vd2mdu	975	11	FDA	FDA	NNP
cord-014597-66vd2mdu	975	12	has	have	VBZ
cord-014597-66vd2mdu	975	13	initiated	initiate	VBN
cord-014597-66vd2mdu	975	14	the	the	DT
cord-014597-66vd2mdu	975	15	Process	Process	NNP
cord-014597-66vd2mdu	975	16	Analytical	Analytical	NNP
cord-014597-66vd2mdu	975	17	Technology	Technology	NNP
cord-014597-66vd2mdu	975	18	(	(	-LRB-
cord-014597-66vd2mdu	975	19	PAT	PAT	NNP
cord-014597-66vd2mdu	975	20	)	)	-RRB-
cord-014597-66vd2mdu	975	21	framework	framework	NN
cord-014597-66vd2mdu	975	22	in	in	IN
cord-014597-66vd2mdu	975	23	order	order	NN
cord-014597-66vd2mdu	975	24	to	to	TO
cord-014597-66vd2mdu	975	25	encourage	encourage	VB
cord-014597-66vd2mdu	975	26	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	975	27	industries	industry	NNS
cord-014597-66vd2mdu	975	28	to	to	TO
cord-014597-66vd2mdu	975	29	use	use	VB
cord-014597-66vd2mdu	975	30	innovative	innovative	JJ
cord-014597-66vd2mdu	975	31	technologies	technology	NNS
cord-014597-66vd2mdu	975	32	to	to	TO
cord-014597-66vd2mdu	975	33	monitor	monitor	VB
cord-014597-66vd2mdu	975	34	in	in	IN
cord-014597-66vd2mdu	975	35	real	real	JJ
cord-014597-66vd2mdu	975	36	time	time	NN
cord-014597-66vd2mdu	975	37	the	the	DT
cord-014597-66vd2mdu	975	38	critical	critical	JJ
cord-014597-66vd2mdu	975	39	process	process	NN
cord-014597-66vd2mdu	975	40	parameters	parameter	NNS
cord-014597-66vd2mdu	975	41	(	(	-LRB-
cord-014597-66vd2mdu	975	42	CPPs	CPPs	NNPS
cord-014597-66vd2mdu	975	43	)	)	-RRB-
cord-014597-66vd2mdu	975	44	,	,	,
cord-014597-66vd2mdu	975	45	and	and	CC
cord-014597-66vd2mdu	975	46	to	to	TO
cord-014597-66vd2mdu	975	47	ensure	ensure	VB
cord-014597-66vd2mdu	975	48	the	the	DT
cord-014597-66vd2mdu	975	49	final	final	JJ
cord-014597-66vd2mdu	975	50	product	product	NN
cord-014597-66vd2mdu	975	51	quality	quality	NN
cord-014597-66vd2mdu	975	52	[	[	-LRB-
cord-014597-66vd2mdu	975	53	1	1	CD
cord-014597-66vd2mdu	975	54	]	]	-RRB-
cord-014597-66vd2mdu	975	55	.	.	.
cord-014597-66vd2mdu	976	1	One	one	CD
cord-014597-66vd2mdu	976	2	of	of	IN
cord-014597-66vd2mdu	976	3	the	the	DT
cord-014597-66vd2mdu	976	4	most	most	RBS
cord-014597-66vd2mdu	976	5	important	important	JJ
cord-014597-66vd2mdu	976	6	CPPs	cpp	NNS
cord-014597-66vd2mdu	976	7	for	for	IN
cord-014597-66vd2mdu	976	8	cell	cell	NN
cord-014597-66vd2mdu	976	9	culture	culture	NN
cord-014597-66vd2mdu	976	10	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	976	11	is	be	VBZ
cord-014597-66vd2mdu	976	12	the	the	DT
cord-014597-66vd2mdu	976	13	specific	specific	JJ
cord-014597-66vd2mdu	976	14	growth	growth	NN
cord-014597-66vd2mdu	976	15	rate	rate	NN
cord-014597-66vd2mdu	976	16	(	(	-LRB-
cord-014597-66vd2mdu	976	17	μ	μ	NN
cord-014597-66vd2mdu	976	18	)	)	-RRB-
cord-014597-66vd2mdu	976	19	,	,	,
cord-014597-66vd2mdu	976	20	which	which	WDT
cord-014597-66vd2mdu	976	21	is	be	VBZ
cord-014597-66vd2mdu	976	22	a	a	DT
cord-014597-66vd2mdu	976	23	direct	direct	JJ
cord-014597-66vd2mdu	976	24	indicator	indicator	NN
cord-014597-66vd2mdu	976	25	of	of	IN
cord-014597-66vd2mdu	976	26	cellular	cellular	JJ
cord-014597-66vd2mdu	976	27	physiological	physiological	JJ
cord-014597-66vd2mdu	976	28	state	state	NN
cord-014597-66vd2mdu	976	29	.	.	.
cord-014597-66vd2mdu	977	1	Indeed	indeed	RB
cord-014597-66vd2mdu	977	2	,	,	,
cord-014597-66vd2mdu	977	3	μ	μ	NN
cord-014597-66vd2mdu	977	4	is	be	VBZ
cord-014597-66vd2mdu	977	5	sensible	sensible	JJ
cord-014597-66vd2mdu	977	6	to	to	IN
cord-014597-66vd2mdu	977	7	culture	culture	NN
cord-014597-66vd2mdu	977	8	conditions	condition	NNS
cord-014597-66vd2mdu	977	9	and	and	CC
cord-014597-66vd2mdu	977	10	its	-PRON-	PRP$
cord-014597-66vd2mdu	977	11	value	value	NN
cord-014597-66vd2mdu	977	12	decreases	decrease	VBZ
cord-014597-66vd2mdu	977	13	when	when	WRB
cord-014597-66vd2mdu	977	14	cells	cell	NNS
cord-014597-66vd2mdu	977	15	are	be	VBP
cord-014597-66vd2mdu	977	16	in	in	IN
cord-014597-66vd2mdu	977	17	the	the	DT
cord-014597-66vd2mdu	977	18	unfavourable	unfavourable	JJ
cord-014597-66vd2mdu	977	19	environment	environment	NN
cord-014597-66vd2mdu	977	20	for	for	IN
cord-014597-66vd2mdu	977	21	growth	growth	NN
cord-014597-66vd2mdu	977	22	[	[	-LRB-
cord-014597-66vd2mdu	977	23	2	2	CD
cord-014597-66vd2mdu	977	24	]	]	-RRB-
cord-014597-66vd2mdu	977	25	,	,	,
cord-014597-66vd2mdu	977	26	which	which	WDT
cord-014597-66vd2mdu	977	27	may	may	MD
cord-014597-66vd2mdu	977	28	greatly	greatly	RB
cord-014597-66vd2mdu	977	29	influence	influence	VB
cord-014597-66vd2mdu	977	30	mAb	mab	NN
cord-014597-66vd2mdu	977	31	production	production	NN
cord-014597-66vd2mdu	977	32	and	and	CC
cord-014597-66vd2mdu	977	33	quality	quality	NN
cord-014597-66vd2mdu	977	34	.	.	.
cord-014597-66vd2mdu	978	1	However	however	RB
cord-014597-66vd2mdu	978	2	,	,	,
cord-014597-66vd2mdu	978	3	until	until	IN
cord-014597-66vd2mdu	978	4	this	this	DT
cord-014597-66vd2mdu	978	5	day	day	NN
cord-014597-66vd2mdu	978	6	,	,	,
cord-014597-66vd2mdu	978	7	the	the	DT
cord-014597-66vd2mdu	978	8	online	online	JJ
cord-014597-66vd2mdu	978	9	monitoring	monitoring	NN
cord-014597-66vd2mdu	978	10	of	of	IN
cord-014597-66vd2mdu	978	11	μ	μ	NN
cord-014597-66vd2mdu	978	12	remains	remain	VBZ
cord-014597-66vd2mdu	978	13	a	a	DT
cord-014597-66vd2mdu	978	14	great	great	JJ
cord-014597-66vd2mdu	978	15	challenge	challenge	NN
cord-014597-66vd2mdu	978	16	for	for	IN
cord-014597-66vd2mdu	978	17	mammalian	mammalian	JJ
cord-014597-66vd2mdu	978	18	cell	cell	NN
cord-014597-66vd2mdu	978	19	culture	culture	NN
cord-014597-66vd2mdu	978	20	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	978	21	.	.	.
cord-014597-66vd2mdu	979	1	IgG	IgG	NNP
cord-014597-66vd2mdu	979	2	-	-	HYPH
cord-014597-66vd2mdu	979	3	producing	produce	VBG
cord-014597-66vd2mdu	979	4	CHO	CHO	NNP
cord-014597-66vd2mdu	979	5	cells	cell	NNS
cord-014597-66vd2mdu	979	6	were	be	VBD
cord-014597-66vd2mdu	979	7	cultured	culture	VBN
cord-014597-66vd2mdu	979	8	in	in	IN
cord-014597-66vd2mdu	979	9	2	2	CD
cord-014597-66vd2mdu	979	10	L	l	NN
cord-014597-66vd2mdu	979	11	stirred	stir	VBD
cord-014597-66vd2mdu	979	12	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	979	13	equipped	equip	VBN
cord-014597-66vd2mdu	979	14	with	with	IN
cord-014597-66vd2mdu	979	15	an	an	DT
cord-014597-66vd2mdu	979	16	in	in	IN
cord-014597-66vd2mdu	979	17	situ	situ	NN
cord-014597-66vd2mdu	979	18	dielectric	dielectric	NN
cord-014597-66vd2mdu	979	19	spectroscopy	spectroscopy	NN
cord-014597-66vd2mdu	979	20	(	(	-LRB-
cord-014597-66vd2mdu	979	21	Hamilton	Hamilton	NNP
cord-014597-66vd2mdu	979	22	)	)	-RRB-
cord-014597-66vd2mdu	979	23	.	.	.
cord-014597-66vd2mdu	980	1	Operating	operating	NN
cord-014597-66vd2mdu	980	2	conditions	condition	NNS
cord-014597-66vd2mdu	980	3	were	be	VBD
cord-014597-66vd2mdu	980	4	fixed	fix	VBN
cord-014597-66vd2mdu	980	5	at	at	IN
cord-014597-66vd2mdu	980	6	90	90	CD
cord-014597-66vd2mdu	980	7	rpm	rpm	NN
cord-014597-66vd2mdu	980	8	,	,	,
cord-014597-66vd2mdu	980	9	50	50	CD
cord-014597-66vd2mdu	980	10	%	%	NN
cord-014597-66vd2mdu	980	11	of	of	IN
cord-014597-66vd2mdu	980	12	air	air	NN
cord-014597-66vd2mdu	980	13	saturation	saturation	NN
cord-014597-66vd2mdu	980	14	,	,	,
cord-014597-66vd2mdu	980	15	pH	pH	NNP
cord-014597-66vd2mdu	980	16	7.2	7.2	CD
cord-014597-66vd2mdu	980	17	and	and	CC
cord-014597-66vd2mdu	980	18	37	37	CD
cord-014597-66vd2mdu	980	19	°	°	NN
cord-014597-66vd2mdu	980	20	C	c	NN
cord-014597-66vd2mdu	980	21	.	.	.
cord-014597-66vd2mdu	981	1	Permittivity	permittivity	NN
cord-014597-66vd2mdu	981	2	of	of	IN
cord-014597-66vd2mdu	981	3	cell	cell	NN
cord-014597-66vd2mdu	981	4	culture	culture	NN
cord-014597-66vd2mdu	981	5	was	be	VBD
cord-014597-66vd2mdu	981	6	measured	measure	VBN
cord-014597-66vd2mdu	981	7	every	every	DT
cord-014597-66vd2mdu	981	8	12	12	CD
cord-014597-66vd2mdu	981	9	min	min	NN
cord-014597-66vd2mdu	981	10	,	,	,
cord-014597-66vd2mdu	981	11	which	which	WDT
cord-014597-66vd2mdu	981	12	allowed	allow	VBD
cord-014597-66vd2mdu	981	13	to	to	TO
cord-014597-66vd2mdu	981	14	calculate	calculate	VB
cord-014597-66vd2mdu	981	15	in	in	IN
cord-014597-66vd2mdu	981	16	real	real	JJ
cord-014597-66vd2mdu	981	17	time	time	NN
cord-014597-66vd2mdu	981	18	the	the	DT
cord-014597-66vd2mdu	981	19	VCD	vcd	NN
cord-014597-66vd2mdu	981	20	by	by	IN
cord-014597-66vd2mdu	981	21	using	use	VBG
cord-014597-66vd2mdu	981	22	a	a	DT
cord-014597-66vd2mdu	981	23	previously	previously	RB
cord-014597-66vd2mdu	981	24	established	establish	VBN
cord-014597-66vd2mdu	981	25	linear	linear	JJ
cord-014597-66vd2mdu	981	26	correlation	correlation	NN
cord-014597-66vd2mdu	981	27	.	.	.
cord-014597-66vd2mdu	982	1	Then	then	RB
cord-014597-66vd2mdu	982	2	,	,	,
cord-014597-66vd2mdu	982	3	a	a	DT
cord-014597-66vd2mdu	982	4	model	model	NN
cord-014597-66vd2mdu	982	5	of	of	IN
cord-014597-66vd2mdu	982	6	online	online	JJ
cord-014597-66vd2mdu	982	7	estimation	estimation	NN
cord-014597-66vd2mdu	982	8	of	of	IN
cord-014597-66vd2mdu	982	9	μ	μ	NN
cord-014597-66vd2mdu	982	10	was	be	VBD
cord-014597-66vd2mdu	982	11	developed	develop	VBN
cord-014597-66vd2mdu	982	12	based	base	VBN
cord-014597-66vd2mdu	982	13	on	on	IN
cord-014597-66vd2mdu	982	14	VCD	vcd	NN
cord-014597-66vd2mdu	982	15	prediction	prediction	NN
cord-014597-66vd2mdu	982	16	and	and	CC
cord-014597-66vd2mdu	982	17	cell	cell	NN
cord-014597-66vd2mdu	982	18	mass	mass	NN
cord-014597-66vd2mdu	982	19	balance	balance	NN
cord-014597-66vd2mdu	982	20	equations	equation	NNS
cord-014597-66vd2mdu	982	21	.	.	.
cord-014597-66vd2mdu	983	1	Several	several	JJ
cord-014597-66vd2mdu	983	2	signal	signal	NN
cord-014597-66vd2mdu	983	3	noise	noise	NN
cord-014597-66vd2mdu	983	4	filters	filter	NNS
cord-014597-66vd2mdu	983	5	and	and	CC
cord-014597-66vd2mdu	983	6	various	various	JJ
cord-014597-66vd2mdu	983	7	calculation	calculation	NN
cord-014597-66vd2mdu	983	8	methods	method	NNS
cord-014597-66vd2mdu	983	9	were	be	VBD
cord-014597-66vd2mdu	983	10	evaluated	evaluate	VBN
cord-014597-66vd2mdu	983	11	to	to	TO
cord-014597-66vd2mdu	983	12	reach	reach	VB
cord-014597-66vd2mdu	983	13	better	well	JJR
cord-014597-66vd2mdu	983	14	model	model	NN
cord-014597-66vd2mdu	983	15	stability	stability	NN
cord-014597-66vd2mdu	983	16	.	.	.
cord-014597-66vd2mdu	984	1	Cell	cell	NN
cord-014597-66vd2mdu	984	2	cultures	culture	NNS
cord-014597-66vd2mdu	984	3	were	be	VBD
cord-014597-66vd2mdu	984	4	performed	perform	VBN
cord-014597-66vd2mdu	984	5	in	in	IN
cord-014597-66vd2mdu	984	6	both	both	DT
cord-014597-66vd2mdu	984	7	batch	batch	NN
cord-014597-66vd2mdu	984	8	and	and	CC
cord-014597-66vd2mdu	984	9	feed	feed	NN
cord-014597-66vd2mdu	984	10	-	-	HYPH
cord-014597-66vd2mdu	984	11	harvest	harvest	NN
cord-014597-66vd2mdu	984	12	modes	mode	NNS
cord-014597-66vd2mdu	984	13	.	.	.
cord-014597-66vd2mdu	985	1	Feed	feed	NN
cord-014597-66vd2mdu	985	2	-	-	HYPH
cord-014597-66vd2mdu	985	3	harvest	harvest	NN
cord-014597-66vd2mdu	985	4	cultures	culture	NNS
cord-014597-66vd2mdu	985	5	consisted	consist	VBN
cord-014597-66vd2mdu	985	6	of	of	IN
cord-014597-66vd2mdu	985	7	sequential	sequential	JJ
cord-014597-66vd2mdu	985	8	renewals	renewal	NNS
cord-014597-66vd2mdu	985	9	of	of	IN
cord-014597-66vd2mdu	985	10	2/3	2/3	CD
cord-014597-66vd2mdu	985	11	volume	volume	NN
cord-014597-66vd2mdu	985	12	of	of	IN
cord-014597-66vd2mdu	985	13	the	the	DT
cord-014597-66vd2mdu	985	14	culture	culture	NN
cord-014597-66vd2mdu	985	15	medium	medium	NN
cord-014597-66vd2mdu	985	16	by	by	IN
cord-014597-66vd2mdu	985	17	following	follow	VBG
cord-014597-66vd2mdu	985	18	different	different	JJ
cord-014597-66vd2mdu	985	19	strategies	strategy	NNS
cord-014597-66vd2mdu	985	20	.	.	.
cord-014597-66vd2mdu	986	1	This	this	DT
cord-014597-66vd2mdu	986	2	study	study	NN
cord-014597-66vd2mdu	986	3	proposed	propose	VBD
cord-014597-66vd2mdu	986	4	an	an	DT
cord-014597-66vd2mdu	986	5	innovative	innovative	JJ
cord-014597-66vd2mdu	986	6	methodology	methodology	NN
cord-014597-66vd2mdu	986	7	based	base	VBN
cord-014597-66vd2mdu	986	8	on	on	IN
cord-014597-66vd2mdu	986	9	dielectric	dielectric	JJ
cord-014597-66vd2mdu	986	10	spectroscopy	spectroscopy	NN
cord-014597-66vd2mdu	986	11	to	to	TO
cord-014597-66vd2mdu	986	12	monitor	monitor	VB
cord-014597-66vd2mdu	986	13	in	in	IN
cord-014597-66vd2mdu	986	14	real	real	JJ
cord-014597-66vd2mdu	986	15	time	time	NN
cord-014597-66vd2mdu	986	16	the	the	DT
cord-014597-66vd2mdu	986	17	cellular	cellular	JJ
cord-014597-66vd2mdu	986	18	physiological	physiological	JJ
cord-014597-66vd2mdu	986	19	state	state	NN
cord-014597-66vd2mdu	986	20	,	,	,
cord-014597-66vd2mdu	986	21	by	by	IN
cord-014597-66vd2mdu	986	22	online	online	JJ
cord-014597-66vd2mdu	986	23	estimating	estimate	VBG
cord-014597-66vd2mdu	986	24	the	the	DT
cord-014597-66vd2mdu	986	25	specific	specific	JJ
cord-014597-66vd2mdu	986	26	growth	growth	NN
cord-014597-66vd2mdu	986	27	rate	rate	NN
cord-014597-66vd2mdu	986	28	(	(	-LRB-
cord-014597-66vd2mdu	986	29	μ	μ	NN
cord-014597-66vd2mdu	986	30	)	)	-RRB-
cord-014597-66vd2mdu	986	31	of	of	IN
cord-014597-66vd2mdu	986	32	cells	cell	NNS
cord-014597-66vd2mdu	986	33	.	.	.
cord-014597-66vd2mdu	987	1	Model	model	NN
cord-014597-66vd2mdu	987	2	of	of	IN
cord-014597-66vd2mdu	987	3	online	online	JJ
cord-014597-66vd2mdu	987	4	estimation	estimation	NN
cord-014597-66vd2mdu	987	5	of	of	IN
cord-014597-66vd2mdu	987	6	μ	μ	NN
cord-014597-66vd2mdu	987	7	was	be	VBD
cord-014597-66vd2mdu	987	8	developed	develop	VBN
cord-014597-66vd2mdu	987	9	from	from	IN
cord-014597-66vd2mdu	987	10	cultures	culture	NNS
cord-014597-66vd2mdu	987	11	in	in	IN
cord-014597-66vd2mdu	987	12	batch	batch	NN
cord-014597-66vd2mdu	987	13	mode	mode	NN
cord-014597-66vd2mdu	987	14	,	,	,
cord-014597-66vd2mdu	987	15	and	and	CC
cord-014597-66vd2mdu	987	16	was	be	VBD
cord-014597-66vd2mdu	987	17	validated	validate	VBN
cord-014597-66vd2mdu	987	18	by	by	IN
cord-014597-66vd2mdu	987	19	comparing	compare	VBG
cord-014597-66vd2mdu	987	20	online	online	RB
cord-014597-66vd2mdu	987	21	estimated	estimate	VBN
cord-014597-66vd2mdu	987	22	μ	μ	NN
cord-014597-66vd2mdu	987	23	with	with	IN
cord-014597-66vd2mdu	987	24	the	the	DT
cord-014597-66vd2mdu	987	25	experimental	experimental	JJ
cord-014597-66vd2mdu	987	26	ones	one	NNS
cord-014597-66vd2mdu	987	27	calculated	calculate	VBN
cord-014597-66vd2mdu	987	28	at	at	IN
cord-014597-66vd2mdu	987	29	the	the	DT
cord-014597-66vd2mdu	987	30	end	end	NN
cord-014597-66vd2mdu	987	31	of	of	IN
cord-014597-66vd2mdu	987	32	the	the	DT
cord-014597-66vd2mdu	987	33	culture	culture	NN
cord-014597-66vd2mdu	987	34	.	.	.
cord-014597-66vd2mdu	988	1	With	with	IN
cord-014597-66vd2mdu	988	2	this	this	DT
cord-014597-66vd2mdu	988	3	model	model	NN
cord-014597-66vd2mdu	988	4	,	,	,
cord-014597-66vd2mdu	988	5	the	the	DT
cord-014597-66vd2mdu	988	6	moment	moment	NN
cord-014597-66vd2mdu	988	7	when	when	WRB
cord-014597-66vd2mdu	988	8	μ	μ	NNP
cord-014597-66vd2mdu	988	9	started	start	VBD
cord-014597-66vd2mdu	988	10	to	to	TO
cord-014597-66vd2mdu	988	11	decrease	decrease	VB
cord-014597-66vd2mdu	988	12	significantly	significantly	RB
cord-014597-66vd2mdu	988	13	,	,	,
cord-014597-66vd2mdu	988	14	which	which	WDT
cord-014597-66vd2mdu	988	15	indicated	indicate	VBD
cord-014597-66vd2mdu	988	16	that	that	IN
cord-014597-66vd2mdu	988	17	cells	cell	NNS
cord-014597-66vd2mdu	988	18	were	be	VBD
cord-014597-66vd2mdu	988	19	no	no	RB
cord-014597-66vd2mdu	988	20	longer	long	RBR
cord-014597-66vd2mdu	988	21	in	in	IN
cord-014597-66vd2mdu	988	22	the	the	DT
cord-014597-66vd2mdu	988	23	exponential	exponential	JJ
cord-014597-66vd2mdu	988	24	growth	growth	NN
cord-014597-66vd2mdu	988	25	phase	phase	NN
cord-014597-66vd2mdu	988	26	,	,	,
cord-014597-66vd2mdu	988	27	was	be	VBD
cord-014597-66vd2mdu	988	28	identified	identify	VBN
cord-014597-66vd2mdu	988	29	as	as	IN
cord-014597-66vd2mdu	988	30	the	the	DT
cord-014597-66vd2mdu	988	31	critical	critical	JJ
cord-014597-66vd2mdu	988	32	moment	moment	NN
cord-014597-66vd2mdu	988	33	.	.	.
cord-014597-66vd2mdu	989	1	To	to	TO
cord-014597-66vd2mdu	989	2	demonstrate	demonstrate	VB
cord-014597-66vd2mdu	989	3	the	the	DT
cord-014597-66vd2mdu	989	4	interest	interest	NN
cord-014597-66vd2mdu	989	5	of	of	IN
cord-014597-66vd2mdu	989	6	online	online	JJ
cord-014597-66vd2mdu	989	7	estimation	estimation	NN
cord-014597-66vd2mdu	989	8	of	of	IN
cord-014597-66vd2mdu	989	9	μ	μ	NN
cord-014597-66vd2mdu	989	10	,	,	,
cord-014597-66vd2mdu	989	11	the	the	DT
cord-014597-66vd2mdu	989	12	developed	develop	VBN
cord-014597-66vd2mdu	989	13	model	model	NN
cord-014597-66vd2mdu	989	14	was	be	VBD
cord-014597-66vd2mdu	989	15	applied	apply	VBN
cord-014597-66vd2mdu	989	16	to	to	IN
cord-014597-66vd2mdu	989	17	a	a	DT
cord-014597-66vd2mdu	989	18	feedharvest	feedharv	JJS
cord-014597-66vd2mdu	989	19	culture	culture	NN
cord-014597-66vd2mdu	989	20	,	,	,
cord-014597-66vd2mdu	989	21	where	where	WRB
cord-014597-66vd2mdu	989	22	the	the	DT
cord-014597-66vd2mdu	989	23	medium	medium	JJ
cord-014597-66vd2mdu	989	24	renewals	renewal	NNS
cord-014597-66vd2mdu	989	25	were	be	VBD
cord-014597-66vd2mdu	989	26	performed	perform	VBN
cord-014597-66vd2mdu	989	27	at	at	IN
cord-014597-66vd2mdu	989	28	the	the	DT
cord-014597-66vd2mdu	989	29	critical	critical	JJ
cord-014597-66vd2mdu	989	30	moments	moment	NNS
cord-014597-66vd2mdu	989	31	indicated	indicate	VBN
cord-014597-66vd2mdu	989	32	by	by	IN
cord-014597-66vd2mdu	989	33	the	the	DT
cord-014597-66vd2mdu	989	34	model	model	NN
cord-014597-66vd2mdu	989	35	.	.	.
cord-014597-66vd2mdu	990	1	This	this	DT
cord-014597-66vd2mdu	990	2	culture	culture	NN
cord-014597-66vd2mdu	990	3	was	be	VBD
cord-014597-66vd2mdu	990	4	then	then	RB
cord-014597-66vd2mdu	990	5	compared	compare	VBN
cord-014597-66vd2mdu	990	6	with	with	IN
cord-014597-66vd2mdu	990	7	the	the	DT
cord-014597-66vd2mdu	990	8	traditional	traditional	JJ
cord-014597-66vd2mdu	990	9	feed	feed	NN
cord-014597-66vd2mdu	990	10	-	-	HYPH
cord-014597-66vd2mdu	990	11	harvest	harvest	NN
cord-014597-66vd2mdu	990	12	culture	culture	NN
cord-014597-66vd2mdu	990	13	where	where	WRB
cord-014597-66vd2mdu	990	14	medium	medium	JJ
cord-014597-66vd2mdu	990	15	renewals	renewal	NNS
cord-014597-66vd2mdu	990	16	were	be	VBD
cord-014597-66vd2mdu	990	17	performed	perform	VBN
cord-014597-66vd2mdu	990	18	by	by	IN
cord-014597-66vd2mdu	990	19	following	follow	VBG
cord-014597-66vd2mdu	990	20	offline	offline	JJ
cord-014597-66vd2mdu	990	21	measurements	measurement	NNS
cord-014597-66vd2mdu	990	22	of	of	IN
cord-014597-66vd2mdu	990	23	glucose	glucose	NN
cord-014597-66vd2mdu	990	24	and	and	CC
cord-014597-66vd2mdu	990	25	glutamine	glutamine	NN
cord-014597-66vd2mdu	990	26	.	.	.
cord-014597-66vd2mdu	991	1	We	-PRON-	PRP
cord-014597-66vd2mdu	991	2	found	find	VBD
cord-014597-66vd2mdu	991	3	that	that	IN
cord-014597-66vd2mdu	991	4	the	the	DT
cord-014597-66vd2mdu	991	5	online	online	JJ
cord-014597-66vd2mdu	991	6	strategy	strategy	NN
cord-014597-66vd2mdu	991	7	allowed	allow	VBN
cord-014597-66vd2mdu	991	8	to	to	TO
cord-014597-66vd2mdu	991	9	maintain	maintain	VB
cord-014597-66vd2mdu	991	10	the	the	DT
cord-014597-66vd2mdu	991	11	value	value	NN
cord-014597-66vd2mdu	991	12	of	of	IN
cord-014597-66vd2mdu	991	13	μ	μ	NN
cord-014597-66vd2mdu	991	14	by	by	IN
cord-014597-66vd2mdu	991	15	renewing	renew	VBG
cord-014597-66vd2mdu	991	16	the	the	DT
cord-014597-66vd2mdu	991	17	medium	medium	NN
cord-014597-66vd2mdu	991	18	at	at	IN
cord-014597-66vd2mdu	991	19	the	the	DT
cord-014597-66vd2mdu	991	20	right	right	JJ
cord-014597-66vd2mdu	991	21	time	time	NN
cord-014597-66vd2mdu	991	22	,	,	,
cord-014597-66vd2mdu	991	23	while	while	IN
cord-014597-66vd2mdu	991	24	the	the	DT
cord-014597-66vd2mdu	991	25	values	value	NNS
cord-014597-66vd2mdu	991	26	of	of	IN
cord-014597-66vd2mdu	991	27	μ	μ	NN
cord-014597-66vd2mdu	991	28	varied	vary	VBD
cord-014597-66vd2mdu	991	29	a	a	DT
cord-014597-66vd2mdu	991	30	lot	lot	NN
cord-014597-66vd2mdu	991	31	when	when	WRB
cord-014597-66vd2mdu	991	32	using	use	VBG
cord-014597-66vd2mdu	991	33	offline	offline	JJ
cord-014597-66vd2mdu	991	34	strategy	strategy	NN
cord-014597-66vd2mdu	991	35	.	.	.
cord-014597-66vd2mdu	992	1	Moreover	moreover	RB
cord-014597-66vd2mdu	992	2	,	,	,
cord-014597-66vd2mdu	992	3	by	by	IN
cord-014597-66vd2mdu	992	4	using	use	VBG
cord-014597-66vd2mdu	992	5	the	the	DT
cord-014597-66vd2mdu	992	6	online	online	JJ
cord-014597-66vd2mdu	992	7	estimation	estimation	NN
cord-014597-66vd2mdu	992	8	of	of	IN
cord-014597-66vd2mdu	992	9	μ	μ	NN
cord-014597-66vd2mdu	992	10	,	,	,
cord-014597-66vd2mdu	992	11	the	the	DT
cord-014597-66vd2mdu	992	12	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	992	13	of	of	IN
cord-014597-66vd2mdu	992	14	IgG	igg	NN
cord-014597-66vd2mdu	992	15	was	be	VBD
cord-014597-66vd2mdu	992	16	kept	keep	VBN
cord-014597-66vd2mdu	992	17	at	at	IN
cord-014597-66vd2mdu	992	18	a	a	DT
cord-014597-66vd2mdu	992	19	high	high	JJ
cord-014597-66vd2mdu	992	20	level	level	NN
cord-014597-66vd2mdu	992	21	(	(	-LRB-
cord-014597-66vd2mdu	992	22	about	about	RB
cord-014597-66vd2mdu	992	23	95	95	CD
cord-014597-66vd2mdu	992	24	%	%	NN
cord-014597-66vd2mdu	992	25	)	)	-RRB-
cord-014597-66vd2mdu	992	26	throughout	throughout	IN
cord-014597-66vd2mdu	992	27	the	the	DT
cord-014597-66vd2mdu	992	28	whole	whole	JJ
cord-014597-66vd2mdu	992	29	culture	culture	NN
cord-014597-66vd2mdu	992	30	.	.	.
cord-014597-66vd2mdu	993	1	However	however	RB
cord-014597-66vd2mdu	993	2	,	,	,
cord-014597-66vd2mdu	993	3	for	for	IN
cord-014597-66vd2mdu	993	4	the	the	DT
cord-014597-66vd2mdu	993	5	culture	culture	NN
cord-014597-66vd2mdu	993	6	using	use	VBG
cord-014597-66vd2mdu	993	7	offline	offline	JJ
cord-014597-66vd2mdu	993	8	strategy	strategy	NN
cord-014597-66vd2mdu	993	9	,	,	,
cord-014597-66vd2mdu	993	10	the	the	DT
cord-014597-66vd2mdu	993	11	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	993	12	level	level	NN
cord-014597-66vd2mdu	993	13	decreased	decrease	VBD
cord-014597-66vd2mdu	993	14	progressively	progressively	RB
cord-014597-66vd2mdu	993	15	and	and	CC
cord-014597-66vd2mdu	993	16	was	be	VBD
cord-014597-66vd2mdu	993	17	only	only	RB
cord-014597-66vd2mdu	993	18	about	about	RB
cord-014597-66vd2mdu	993	19	75	75	CD
cord-014597-66vd2mdu	993	20	%	%	NN
cord-014597-66vd2mdu	993	21	at	at	IN
cord-014597-66vd2mdu	993	22	the	the	DT
cord-014597-66vd2mdu	993	23	end	end	NN
cord-014597-66vd2mdu	993	24	of	of	IN
cord-014597-66vd2mdu	993	25	the	the	DT
cord-014597-66vd2mdu	993	26	culture	culture	NN
cord-014597-66vd2mdu	993	27	(	(	-LRB-
cord-014597-66vd2mdu	993	28	Fig	Fig	NNP
cord-014597-66vd2mdu	993	29	.	.	.
cord-014597-66vd2mdu	993	30	1	1	CD
cord-014597-66vd2mdu	993	31	)	)	-RRB-
cord-014597-66vd2mdu	993	32	.	.	.
cord-014597-66vd2mdu	994	1	Model	model	NN
cord-014597-66vd2mdu	994	2	of	of	IN
cord-014597-66vd2mdu	994	3	online	online	JJ
cord-014597-66vd2mdu	994	4	estimation	estimation	NN
cord-014597-66vd2mdu	994	5	of	of	IN
cord-014597-66vd2mdu	994	6	μ	μ	NN
cord-014597-66vd2mdu	994	7	was	be	VBD
cord-014597-66vd2mdu	994	8	developed	develop	VBN
cord-014597-66vd2mdu	994	9	by	by	IN
cord-014597-66vd2mdu	994	10	using	use	VBG
cord-014597-66vd2mdu	994	11	dielectric	dielectric	NN
cord-014597-66vd2mdu	994	12	spectroscopy	spectroscopy	NN
cord-014597-66vd2mdu	994	13	,	,	,
cord-014597-66vd2mdu	994	14	which	which	WDT
cord-014597-66vd2mdu	994	15	allowed	allow	VBD
cord-014597-66vd2mdu	994	16	to	to	TO
cord-014597-66vd2mdu	994	17	monitor	monitor	VB
cord-014597-66vd2mdu	994	18	the	the	DT
cord-014597-66vd2mdu	994	19	physiological	physiological	JJ
cord-014597-66vd2mdu	994	20	state	state	NN
cord-014597-66vd2mdu	994	21	of	of	IN
cord-014597-66vd2mdu	994	22	cells	cell	NNS
cord-014597-66vd2mdu	994	23	in	in	IN
cord-014597-66vd2mdu	994	24	cell	cell	NN
cord-014597-66vd2mdu	994	25	culture	culture	NN
cord-014597-66vd2mdu	994	26	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	994	27	.	.	.
cord-014597-66vd2mdu	995	1	Implementation	implementation	NN
cord-014597-66vd2mdu	995	2	of	of	IN
cord-014597-66vd2mdu	995	3	this	this	DT
cord-014597-66vd2mdu	995	4	model	model	NN
cord-014597-66vd2mdu	995	5	in	in	IN
cord-014597-66vd2mdu	995	6	feed	feed	NN
cord-014597-66vd2mdu	995	7	-	-	HYPH
cord-014597-66vd2mdu	995	8	harvest	harvest	NN
cord-014597-66vd2mdu	995	9	cell	cell	NN
cord-014597-66vd2mdu	995	10	culture	culture	NN
cord-014597-66vd2mdu	995	11	led	lead	VBD
cord-014597-66vd2mdu	995	12	to	to	IN
cord-014597-66vd2mdu	995	13	better	well	JJR
cord-014597-66vd2mdu	995	14	mAb	mab	NN
cord-014597-66vd2mdu	995	15	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	995	16	,	,	,
cord-014597-66vd2mdu	995	17	which	which	WDT
cord-014597-66vd2mdu	995	18	demonstrates	demonstrate	VBZ
cord-014597-66vd2mdu	995	19	clearly	clearly	RB
cord-014597-66vd2mdu	995	20	the	the	DT
cord-014597-66vd2mdu	995	21	potential	potential	NN
cord-014597-66vd2mdu	995	22	of	of	IN
cord-014597-66vd2mdu	995	23	this	this	DT
cord-014597-66vd2mdu	995	24	methodology	methodology	NN
cord-014597-66vd2mdu	995	25	in	in	IN
cord-014597-66vd2mdu	995	26	mAb	mab	NN
cord-014597-66vd2mdu	995	27	production	production	NN
cord-014597-66vd2mdu	995	28	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	995	29	.	.	.
cord-014597-66vd2mdu	996	1	Background	Background	NNP
cord-014597-66vd2mdu	996	2	Monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	996	3	antibodies	antibody	NNS
cord-014597-66vd2mdu	996	4	are	be	VBP
cord-014597-66vd2mdu	996	5	normally	normally	RB
cord-014597-66vd2mdu	996	6	synthesised	synthesise	VBN
cord-014597-66vd2mdu	996	7	from	from	IN
cord-014597-66vd2mdu	996	8	transfected	transfecte	VBN
cord-014597-66vd2mdu	996	9	mammalian	mammalian	JJ
cord-014597-66vd2mdu	996	10	cells	cell	NNS
cord-014597-66vd2mdu	996	11	as	as	IN
cord-014597-66vd2mdu	996	12	heterogeneous	heterogeneous	JJ
cord-014597-66vd2mdu	996	13	mixtures	mixture	NNS
cord-014597-66vd2mdu	996	14	of	of	IN
cord-014597-66vd2mdu	996	15	glycoforms	glycoform	NNS
cord-014597-66vd2mdu	996	16	[	[	-LRB-
cord-014597-66vd2mdu	996	17	1	1	CD
cord-014597-66vd2mdu	996	18	]	]	-RRB-
cord-014597-66vd2mdu	996	19	.	.	.
cord-014597-66vd2mdu	997	1	However	however	RB
cord-014597-66vd2mdu	997	2	,	,	,
cord-014597-66vd2mdu	997	3	clinical	clinical	JJ
cord-014597-66vd2mdu	997	4	efficacy	efficacy	NN
cord-014597-66vd2mdu	997	5	may	may	MD
cord-014597-66vd2mdu	997	6	depend	depend	VB
cord-014597-66vd2mdu	997	7	upon	upon	IN
cord-014597-66vd2mdu	997	8	single	single	JJ
cord-014597-66vd2mdu	997	9	glycoforms	glycoform	NNS
cord-014597-66vd2mdu	997	10	which	which	WDT
cord-014597-66vd2mdu	997	11	have	have	VBP
cord-014597-66vd2mdu	997	12	been	be	VBN
cord-014597-66vd2mdu	997	13	difficult	difficult	JJ
cord-014597-66vd2mdu	997	14	to	to	TO
cord-014597-66vd2mdu	997	15	isolate	isolate	VB
cord-014597-66vd2mdu	997	16	[	[	-LRB-
cord-014597-66vd2mdu	997	17	2	2	CD
cord-014597-66vd2mdu	997	18	]	]	-RRB-
cord-014597-66vd2mdu	997	19	.	.	.
cord-014597-66vd2mdu	998	1	We	-PRON-	PRP
cord-014597-66vd2mdu	998	2	have	have	VBP
cord-014597-66vd2mdu	998	3	now	now	RB
cord-014597-66vd2mdu	998	4	developed	develop	VBN
cord-014597-66vd2mdu	998	5	an	an	DT
cord-014597-66vd2mdu	998	6	efficient	efficient	JJ
cord-014597-66vd2mdu	998	7	method	method	NN
cord-014597-66vd2mdu	998	8	for	for	IN
cord-014597-66vd2mdu	998	9	generating	generate	VBG
cord-014597-66vd2mdu	998	10	single	single	JJ
cord-014597-66vd2mdu	998	11	glycoforms	glycoform	NNS
cord-014597-66vd2mdu	998	12	by	by	IN
cord-014597-66vd2mdu	998	13	solid	solid	JJ
cord-014597-66vd2mdu	998	14	phase	phase	NN
cord-014597-66vd2mdu	998	15	re	re	NN
cord-014597-66vd2mdu	998	16	-	-	NN
cord-014597-66vd2mdu	998	17	modelling	modelling	NN
cord-014597-66vd2mdu	998	18	which	which	WDT
cord-014597-66vd2mdu	998	19	is	be	VBZ
cord-014597-66vd2mdu	998	20	superior	superior	JJ
cord-014597-66vd2mdu	998	21	to	to	IN
cord-014597-66vd2mdu	998	22	previous	previous	JJ
cord-014597-66vd2mdu	998	23	methods	method	NNS
cord-014597-66vd2mdu	998	24	because	because	IN
cord-014597-66vd2mdu	998	25	it	-PRON-	PRP
cord-014597-66vd2mdu	998	26	allows	allow	VBZ
cord-014597-66vd2mdu	998	27	a	a	DT
cord-014597-66vd2mdu	998	28	sequential	sequential	JJ
cord-014597-66vd2mdu	998	29	series	series	NN
cord-014597-66vd2mdu	998	30	of	of	IN
cord-014597-66vd2mdu	998	31	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	998	32	changes	change	NNS
cord-014597-66vd2mdu	998	33	without	without	IN
cord-014597-66vd2mdu	998	34	the	the	DT
cord-014597-66vd2mdu	998	35	need	need	NN
cord-014597-66vd2mdu	998	36	for	for	IN
cord-014597-66vd2mdu	998	37	intermediate	intermediate	JJ
cord-014597-66vd2mdu	998	38	purification	purification	NN
cord-014597-66vd2mdu	998	39	of	of	IN
cord-014597-66vd2mdu	998	40	the	the	DT
cord-014597-66vd2mdu	998	41	antibody	antibody	NN
cord-014597-66vd2mdu	998	42	.	.	.
cord-014597-66vd2mdu	999	1	Solidphase	solidphase	NN
cord-014597-66vd2mdu	999	2	binding	binding	NN
cord-014597-66vd2mdu	999	3	exposes	expose	VBZ
cord-014597-66vd2mdu	999	4	the	the	DT
cord-014597-66vd2mdu	999	5	antibody	antibody	NN
cord-014597-66vd2mdu	999	6	glycans	glycan	NNS
cord-014597-66vd2mdu	999	7	to	to	TO
cord-014597-66vd2mdu	999	8	enable	enable	VB
cord-014597-66vd2mdu	999	9	easier	easy	JJR
cord-014597-66vd2mdu	999	10	access	access	NN
cord-014597-66vd2mdu	999	11	of	of	IN
cord-014597-66vd2mdu	999	12	the	the	DT
cord-014597-66vd2mdu	999	13	transforming	transform	VBG
cord-014597-66vd2mdu	999	14	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	999	15	enzyme	enzyme	NN
cord-014597-66vd2mdu	999	16	.	.	.
cord-014597-66vd2mdu	1000	1	The	the	DT
cord-014597-66vd2mdu	1000	2	antibodies	antibody	NNS
cord-014597-66vd2mdu	1000	3	subjected	subject	VBN
cord-014597-66vd2mdu	1000	4	to	to	IN
cord-014597-66vd2mdu	1000	5	modification	modification	NN
cord-014597-66vd2mdu	1000	6	were	be	VBD
cord-014597-66vd2mdu	1000	7	a	a	DT
cord-014597-66vd2mdu	1000	8	chimeric	chimeric	JJ
cord-014597-66vd2mdu	1000	9	human/	human/	CD
cord-014597-66vd2mdu	1000	10	camelid	camelid	NN
cord-014597-66vd2mdu	1000	11	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1000	12	antibody	antibody	NN
cord-014597-66vd2mdu	1000	13	(	(	-LRB-
cord-014597-66vd2mdu	1000	14	EG2	EG2	NNP
cord-014597-66vd2mdu	1000	15	)	)	-RRB-
cord-014597-66vd2mdu	1000	16	,	,	,
cord-014597-66vd2mdu	1000	17	a	a	DT
cord-014597-66vd2mdu	1000	18	humanized	humanize	VBN
cord-014597-66vd2mdu	1000	19	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1000	20	antibody	antibody	NN
cord-014597-66vd2mdu	1000	21	(	(	-LRB-
cord-014597-66vd2mdu	1000	22	IL8	IL8	NNP
cord-014597-66vd2mdu	1000	23	)	)	-RRB-
cord-014597-66vd2mdu	1000	24	,	,	,
cord-014597-66vd2mdu	1000	25	a	a	DT
cord-014597-66vd2mdu	1000	26	full	full	JJ
cord-014597-66vd2mdu	1000	27	size	size	NN
cord-014597-66vd2mdu	1000	28	chimeric	chimeric	JJ
cord-014597-66vd2mdu	1000	29	antibody	antibody	NN
cord-014597-66vd2mdu	1000	30	(	(	-LRB-
cord-014597-66vd2mdu	1000	31	Cetuximab	Cetuximab	NNP
cord-014597-66vd2mdu	1000	32	)	)	-RRB-
cord-014597-66vd2mdu	1000	33	and	and	CC
cord-014597-66vd2mdu	1000	34	polyclonal	polyclonal	JJ
cord-014597-66vd2mdu	1000	35	antibodies	antibody	NNS
cord-014597-66vd2mdu	1000	36	obtained	obtain	VBN
cord-014597-66vd2mdu	1000	37	from	from	IN
cord-014597-66vd2mdu	1000	38	pooled	pool	VBN
cord-014597-66vd2mdu	1000	39	human	human	JJ
cord-014597-66vd2mdu	1000	40	serum	serum	NN
cord-014597-66vd2mdu	1000	41	.	.	.
cord-014597-66vd2mdu	1001	1	The	the	DT
cord-014597-66vd2mdu	1001	2	antibodies	antibody	NNS
cord-014597-66vd2mdu	1001	3	were	be	VBD
cord-014597-66vd2mdu	1001	4	bound	bind	VBN
cord-014597-66vd2mdu	1001	5	to	to	IN
cord-014597-66vd2mdu	1001	6	a	a	DT
cord-014597-66vd2mdu	1001	7	Protein	Protein	NNP
cord-014597-66vd2mdu	1001	8	A	a	DT
cord-014597-66vd2mdu	1001	9	column	column	NN
cord-014597-66vd2mdu	1001	10	using	use	VBG
cord-014597-66vd2mdu	1001	11	conditions	condition	NNS
cord-014597-66vd2mdu	1001	12	typical	typical	JJ
cord-014597-66vd2mdu	1001	13	of	of	IN
cord-014597-66vd2mdu	1001	14	Mab	Mab	NNP
cord-014597-66vd2mdu	1001	15	purification	purification	NN
cord-014597-66vd2mdu	1001	16	(	(	-LRB-
cord-014597-66vd2mdu	1001	17	Fig	Fig	NNP
cord-014597-66vd2mdu	1001	18	.	.	.
cord-014597-66vd2mdu	1001	19	1	1	CD
cord-014597-66vd2mdu	1001	20	)	)	-RRB-
cord-014597-66vd2mdu	1001	21	.	.	.
cord-014597-66vd2mdu	1002	1	After	after	IN
cord-014597-66vd2mdu	1002	2	washing	wash	VBG
cord-014597-66vd2mdu	1002	3	out	out	RP
cord-014597-66vd2mdu	1002	4	non	non	JJ
cord-014597-66vd2mdu	1002	5	-	-	JJ
cord-014597-66vd2mdu	1002	6	bound	bound	JJ
cord-014597-66vd2mdu	1002	7	impurities	impurity	NNS
cord-014597-66vd2mdu	1002	8	by	by	IN
cord-014597-66vd2mdu	1002	9	a	a	DT
cord-014597-66vd2mdu	1002	10	neutral	neutral	JJ
cord-014597-66vd2mdu	1002	11	pH	pH	NNP
cord-014597-66vd2mdu	1002	12	buffer	buffer	NN
cord-014597-66vd2mdu	1002	13	,	,	,
cord-014597-66vd2mdu	1002	14	each	each	DT
cord-014597-66vd2mdu	1002	15	antibody	antibody	NN
cord-014597-66vd2mdu	1002	16	was	be	VBD
cord-014597-66vd2mdu	1002	17	subjected	subject	VBN
cord-014597-66vd2mdu	1002	18	to	to	IN
cord-014597-66vd2mdu	1002	19	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	1002	20	modification	modification	NN
cord-014597-66vd2mdu	1002	21	directed	direct	VBN
cord-014597-66vd2mdu	1002	22	to	to	IN
cord-014597-66vd2mdu	1002	23	a	a	DT
cord-014597-66vd2mdu	1002	24	targeted	target	VBN
cord-014597-66vd2mdu	1002	25	glycan	glycan	NN
cord-014597-66vd2mdu	1002	26	profile	profile	NN
cord-014597-66vd2mdu	1002	27	(	(	-LRB-
cord-014597-66vd2mdu	1002	28	Table	table	NN
cord-014597-66vd2mdu	1002	29	1	1	CD
cord-014597-66vd2mdu	1002	30	)	)	-RRB-
cord-014597-66vd2mdu	1002	31	.	.	.
cord-014597-66vd2mdu	1003	1	The	the	DT
cord-014597-66vd2mdu	1003	2	antibodies	antibody	NNS
cord-014597-66vd2mdu	1003	3	were	be	VBD
cord-014597-66vd2mdu	1003	4	then	then	RB
cord-014597-66vd2mdu	1003	5	eluted	elute	VBN
cord-014597-66vd2mdu	1003	6	with	with	IN
cord-014597-66vd2mdu	1003	7	a	a	DT
cord-014597-66vd2mdu	1003	8	low	low	JJ
cord-014597-66vd2mdu	1003	9	pH	ph	NN
cord-014597-66vd2mdu	1003	10	buffer	buffer	NN
cord-014597-66vd2mdu	1003	11	and	and	CC
cord-014597-66vd2mdu	1003	12	neutralized	neutralize	VBN
cord-014597-66vd2mdu	1003	13	.	.	.
cord-014597-66vd2mdu	1004	1	The	the	DT
cord-014597-66vd2mdu	1004	2	glycan	glycan	NN
cord-014597-66vd2mdu	1004	3	profiles	profile	NNS
cord-014597-66vd2mdu	1004	4	were	be	VBD
cord-014597-66vd2mdu	1004	5	analysed	analyse	VBN
cord-014597-66vd2mdu	1004	6	following	follow	VBG
cord-014597-66vd2mdu	1004	7	glycan	glycan	NN
cord-014597-66vd2mdu	1004	8	removal	removal	NN
cord-014597-66vd2mdu	1004	9	with	with	IN
cord-014597-66vd2mdu	1004	10	PNGase	PNGase	NNP
cord-014597-66vd2mdu	1004	11	F	F	NNP
cord-014597-66vd2mdu	1004	12	,	,	,
cord-014597-66vd2mdu	1004	13	labelling	label	VBG
cord-014597-66vd2mdu	1004	14	with	with	IN
cord-014597-66vd2mdu	1004	15	2-aminobenzamide	2-aminobenzamide	CD
cord-014597-66vd2mdu	1004	16	and	and	CC
cord-014597-66vd2mdu	1004	17	separation	separation	NN
cord-014597-66vd2mdu	1004	18	on	on	IN
cord-014597-66vd2mdu	1004	19	a	a	DT
cord-014597-66vd2mdu	1004	20	HILIC	HILIC	NNP
cord-014597-66vd2mdu	1004	21	-	-	HYPH
cord-014597-66vd2mdu	1004	22	HPLC	HPLC	NNP
cord-014597-66vd2mdu	1004	23	column	column	NN
cord-014597-66vd2mdu	1004	24	[	[	-LRB-
cord-014597-66vd2mdu	1004	25	3	3	CD
cord-014597-66vd2mdu	1004	26	]	]	-RRB-
cord-014597-66vd2mdu	1004	27	.	.	.
cord-014597-66vd2mdu	1005	1	Prior	prior	RB
cord-014597-66vd2mdu	1005	2	to	to	IN
cord-014597-66vd2mdu	1005	3	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	1005	4	modification	modification	NN
cord-014597-66vd2mdu	1005	5	glycan	glycan	NN
cord-014597-66vd2mdu	1005	6	analysis	analysis	NN
cord-014597-66vd2mdu	1005	7	of	of	IN
cord-014597-66vd2mdu	1005	8	all	all	DT
cord-014597-66vd2mdu	1005	9	4	4	CD
cord-014597-66vd2mdu	1005	10	antibodies	antibody	NNS
cord-014597-66vd2mdu	1005	11	showed	show	VBD
cord-014597-66vd2mdu	1005	12	variable	variable	JJ
cord-014597-66vd2mdu	1005	13	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1005	14	and	and	CC
cord-014597-66vd2mdu	1005	15	sialylation	sialylation	NN
cord-014597-66vd2mdu	1005	16	typical	typical	JJ
cord-014597-66vd2mdu	1005	17	of	of	IN
cord-014597-66vd2mdu	1005	18	human	human	JJ
cord-014597-66vd2mdu	1005	19	Abs	abs	NN
cord-014597-66vd2mdu	1005	20	.	.	.
cord-014597-66vd2mdu	1006	1	This	this	DT
cord-014597-66vd2mdu	1006	2	included	include	VBD
cord-014597-66vd2mdu	1006	3	a	a	DT
cord-014597-66vd2mdu	1006	4	distribution	distribution	NN
cord-014597-66vd2mdu	1006	5	of	of	IN
cord-014597-66vd2mdu	1006	6	FG0	FG0	NNP
cord-014597-66vd2mdu	1006	7	,	,	,
cord-014597-66vd2mdu	1006	8	FG1	FG1	NNP
cord-014597-66vd2mdu	1006	9	,	,	,
cord-014597-66vd2mdu	1006	10	FG2	FG2	NNP
cord-014597-66vd2mdu	1006	11	,	,	,
cord-014597-66vd2mdu	1006	12	FS1	FS1	NNP
cord-014597-66vd2mdu	1006	13	and	and	CC
cord-014597-66vd2mdu	1006	14	FS2	FS2	NNP
cord-014597-66vd2mdu	1006	15	with	with	IN
cord-014597-66vd2mdu	1006	16	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1006	17	indices	index	NNS
cord-014597-66vd2mdu	1006	18	ranging	range	VBG
cord-014597-66vd2mdu	1006	19	from	from	IN
cord-014597-66vd2mdu	1006	20	0.22	0.22	CD
cord-014597-66vd2mdu	1006	21	for	for	IN
cord-014597-66vd2mdu	1006	22	IL8	il8	NN
cord-014597-66vd2mdu	1006	23	to	to	IN
cord-014597-66vd2mdu	1006	24	0.64	0.64	CD
cord-014597-66vd2mdu	1006	25	for	for	IN
cord-014597-66vd2mdu	1006	26	EG2	EG2	NNP
cord-014597-66vd2mdu	1006	27	.	.	.
cord-014597-66vd2mdu	1007	1	There	there	EX
cord-014597-66vd2mdu	1007	2	was	be	VBD
cord-014597-66vd2mdu	1007	3	minimal	minimal	JJ
cord-014597-66vd2mdu	1007	4	sialylation	sialylation	NN
cord-014597-66vd2mdu	1007	5	in	in	IN
cord-014597-66vd2mdu	1007	6	IL8	IL8	NNP
cord-014597-66vd2mdu	1007	7	but	but	CC
cord-014597-66vd2mdu	1007	8	up	up	IN
cord-014597-66vd2mdu	1007	9	to	to	TO
cord-014597-66vd2mdu	1007	10	11	11	CD
cord-014597-66vd2mdu	1007	11	%	%	NN
cord-014597-66vd2mdu	1007	12	in	in	IN
cord-014597-66vd2mdu	1007	13	EG2	EG2	NNP
cord-014597-66vd2mdu	1007	14	.	.	.
cord-014597-66vd2mdu	1008	1	Glycan	glycan	JJ
cord-014597-66vd2mdu	1008	2	modifications	modification	NNS
cord-014597-66vd2mdu	1008	3	were	be	VBD
cord-014597-66vd2mdu	1008	4	made	make	VBN
cord-014597-66vd2mdu	1008	5	as	as	IN
cord-014597-66vd2mdu	1008	6	each	each	DT
cord-014597-66vd2mdu	1008	7	antibody	antibody	NN
cord-014597-66vd2mdu	1008	8	was	be	VBD
cord-014597-66vd2mdu	1008	9	held	hold	VBN
cord-014597-66vd2mdu	1008	10	on	on	IN
cord-014597-66vd2mdu	1008	11	a	a	DT
cord-014597-66vd2mdu	1008	12	Protein	Protein	NNP
cord-014597-66vd2mdu	1008	13	A	a	DT
cord-014597-66vd2mdu	1008	14	column	column	NN
cord-014597-66vd2mdu	1008	15	in	in	IN
cord-014597-66vd2mdu	1008	16	accordance	accordance	NN
cord-014597-66vd2mdu	1008	17	with	with	IN
cord-014597-66vd2mdu	1008	18	procedures	procedure	NNS
cord-014597-66vd2mdu	1008	19	shown	show	VBN
cord-014597-66vd2mdu	1008	20	in	in	IN
cord-014597-66vd2mdu	1008	21	Table	Table	NNP
cord-014597-66vd2mdu	1008	22	 	 	_SP
cord-014597-66vd2mdu	1008	23	1	1	CD
cord-014597-66vd2mdu	1008	24	.	.	.
cord-014597-66vd2mdu	1009	1	Agalactosylated	agalactosylated	JJ
cord-014597-66vd2mdu	1009	2	glycans	glycan	NNS
cord-014597-66vd2mdu	1009	3	were	be	VBD
cord-014597-66vd2mdu	1009	4	enriched	enrich	VBN
cord-014597-66vd2mdu	1009	5	by	by	IN
cord-014597-66vd2mdu	1009	6	treatment	treatment	NN
cord-014597-66vd2mdu	1009	7	with	with	IN
cord-014597-66vd2mdu	1009	8	the	the	DT
cord-014597-66vd2mdu	1009	9	single	single	JJ
cord-014597-66vd2mdu	1009	10	addition	addition	NN
cord-014597-66vd2mdu	1009	11	of	of	IN
cord-014597-66vd2mdu	1009	12	galactosidase	galactosidase	NN
cord-014597-66vd2mdu	1009	13	and	and	CC
cord-014597-66vd2mdu	1009	14	neuraminidase	neuraminidase	NN
cord-014597-66vd2mdu	1009	15	.	.	.
cord-014597-66vd2mdu	1010	1	This	this	DT
cord-014597-66vd2mdu	1010	2	resulted	result	VBD
cord-014597-66vd2mdu	1010	3	in	in	IN
cord-014597-66vd2mdu	1010	4	83	83	CD
cord-014597-66vd2mdu	1010	5	-	-	SYM
cord-014597-66vd2mdu	1010	6	95	95	CD
cord-014597-66vd2mdu	1010	7	%	%	NN
cord-014597-66vd2mdu	1010	8	of	of	IN
cord-014597-66vd2mdu	1010	9	agalactosylated	agalactosylate	VBN
cord-014597-66vd2mdu	1010	10	structures	structure	NNS
cord-014597-66vd2mdu	1010	11	in	in	IN
cord-014597-66vd2mdu	1010	12	the	the	DT
cord-014597-66vd2mdu	1010	13	Mabs	Mabs	NNP
cord-014597-66vd2mdu	1010	14	and	and	CC
cord-014597-66vd2mdu	1010	15	65	65	CD
cord-014597-66vd2mdu	1010	16	%	%	NN
cord-014597-66vd2mdu	1010	17	in	in	IN
cord-014597-66vd2mdu	1010	18	the	the	DT
cord-014597-66vd2mdu	1010	19	polyclonal	polyclonal	JJ
cord-014597-66vd2mdu	1010	20	antibody	antibody	NN
cord-014597-66vd2mdu	1010	21	.	.	.
cord-014597-66vd2mdu	1011	1	Galactosylated	Galactosylated	NNP
cord-014597-66vd2mdu	1011	2	antibodies	antibody	NNS
cord-014597-66vd2mdu	1011	3	(	(	-LRB-
cord-014597-66vd2mdu	1011	4	>	>	NFP
cord-014597-66vd2mdu	1011	5	95	95	CD
cord-014597-66vd2mdu	1011	6	%	%	NN
cord-014597-66vd2mdu	1011	7	yield	yield	NN
cord-014597-66vd2mdu	1011	8	)	)	-RRB-
cord-014597-66vd2mdu	1011	9	were	be	VBD
cord-014597-66vd2mdu	1011	10	produced	produce	VBN
cord-014597-66vd2mdu	1011	11	by	by	IN
cord-014597-66vd2mdu	1011	12	a	a	DT
cord-014597-66vd2mdu	1011	13	single	single	JJ
cord-014597-66vd2mdu	1011	14	stage	stage	NN
cord-014597-66vd2mdu	1011	15	reaction	reaction	NN
cord-014597-66vd2mdu	1011	16	involving	involve	VBG
cord-014597-66vd2mdu	1011	17	sialidase	sialidase	NN
cord-014597-66vd2mdu	1011	18	and	and	CC
cord-014597-66vd2mdu	1011	19	by	by	IN
cord-014597-66vd2mdu	1011	20	galactosyltransferase	galactosyltransferase	NNP
cord-014597-66vd2mdu	1011	21	with	with	IN
cord-014597-66vd2mdu	1011	22	UDP	UDP	NNP
cord-014597-66vd2mdu	1011	23	-	-	HYPH
cord-014597-66vd2mdu	1011	24	Gal	gal	NN
cord-014597-66vd2mdu	1011	25	.	.	.
cord-014597-66vd2mdu	1012	1	Breakdown	breakdown	NN
cord-014597-66vd2mdu	1012	2	of	of	IN
cord-014597-66vd2mdu	1012	3	the	the	DT
cord-014597-66vd2mdu	1012	4	glycans	glycan	NNS
cord-014597-66vd2mdu	1012	5	to	to	IN
cord-014597-66vd2mdu	1012	6	a	a	DT
cord-014597-66vd2mdu	1012	7	trimannosyl	trimannosyl	NN
cord-014597-66vd2mdu	1012	8	core	core	NN
cord-014597-66vd2mdu	1012	9	was	be	VBD
cord-014597-66vd2mdu	1012	10	accomplished	accomplish	VBN
cord-014597-66vd2mdu	1012	11	by	by	IN
cord-014597-66vd2mdu	1012	12	treatment	treatment	NN
cord-014597-66vd2mdu	1012	13	of	of	IN
cord-014597-66vd2mdu	1012	14	the	the	DT
cord-014597-66vd2mdu	1012	15	agalactosylated	agalactosylate	VBN
cord-014597-66vd2mdu	1012	16	structures	structure	NNS
cord-014597-66vd2mdu	1012	17	with	with	IN
cord-014597-66vd2mdu	1012	18	hexosaminidase	hexosaminidase	NNP
cord-014597-66vd2mdu	1012	19	.	.	.
cord-014597-66vd2mdu	1013	1	This	this	DT
cord-014597-66vd2mdu	1013	2	produced	produce	VBD
cord-014597-66vd2mdu	1013	3	a	a	DT
cord-014597-66vd2mdu	1013	4	yield	yield	NN
cord-014597-66vd2mdu	1013	5	of	of	IN
cord-014597-66vd2mdu	1013	6	76	76	CD
cord-014597-66vd2mdu	1013	7	-	-	SYM
cord-014597-66vd2mdu	1013	8	80	80	CD
cord-014597-66vd2mdu	1013	9	%	%	NN
cord-014597-66vd2mdu	1013	10	of	of	IN
cord-014597-66vd2mdu	1013	11	the	the	DT
cord-014597-66vd2mdu	1013	12	FM3	FM3	NNP
cord-014597-66vd2mdu	1013	13	structure	structure	NN
cord-014597-66vd2mdu	1013	14	with	with	IN
cord-014597-66vd2mdu	1013	15	a	a	DT
cord-014597-66vd2mdu	1013	16	small	small	JJ
cord-014597-66vd2mdu	1013	17	remainder	remainder	NN
cord-014597-66vd2mdu	1013	18	of	of	IN
cord-014597-66vd2mdu	1013	19	FA1	FA1	NNP
cord-014597-66vd2mdu	1013	20	.	.	.
cord-014597-66vd2mdu	1014	1	Sialylated	sialylated	JJ
cord-014597-66vd2mdu	1014	2	antibodies	antibody	NNS
cord-014597-66vd2mdu	1014	3	(	(	-LRB-
cord-014597-66vd2mdu	1014	4	>	>	NFP
cord-014597-66vd2mdu	1014	5	95	95	CD
cord-014597-66vd2mdu	1014	6	%	%	NN
cord-014597-66vd2mdu	1014	7	)	)	-RRB-
cord-014597-66vd2mdu	1014	8	were	be	VBD
cord-014597-66vd2mdu	1014	9	produced	produce	VBN
cord-014597-66vd2mdu	1014	10	by	by	IN
cord-014597-66vd2mdu	1014	11	a	a	DT
cord-014597-66vd2mdu	1014	12	2	2	CD
cord-014597-66vd2mdu	1014	13	stage	stage	NN
cord-014597-66vd2mdu	1014	14	reaction	reaction	NN
cord-014597-66vd2mdu	1014	15	involving	involve	VBG
cord-014597-66vd2mdu	1014	16	sialidase	sialidase	NN
cord-014597-66vd2mdu	1014	17	,	,	,
cord-014597-66vd2mdu	1014	18	galactosyltransferase	galactosyltransferase	NNP
cord-014597-66vd2mdu	1014	19	and	and	CC
cord-014597-66vd2mdu	1014	20	finally	finally	RB
cord-014597-66vd2mdu	1014	21	treatment	treatment	NN
cord-014597-66vd2mdu	1014	22	with	with	IN
cord-014597-66vd2mdu	1014	23	2,6	2,6	CD
cord-014597-66vd2mdu	1014	24	sialyltransferase	sialyltransferase	NN
cord-014597-66vd2mdu	1014	25	in	in	IN
cord-014597-66vd2mdu	1014	26	the	the	DT
cord-014597-66vd2mdu	1014	27	presence	presence	NN
cord-014597-66vd2mdu	1014	28	of	of	IN
cord-014597-66vd2mdu	1014	29	CMP	CMP	NNP
cord-014597-66vd2mdu	1014	30	-	-	HYPH
cord-014597-66vd2mdu	1014	31	NANA	NANA	NNP
cord-014597-66vd2mdu	1014	32	.	.	.
cord-014597-66vd2mdu	1015	1	The	the	DT
cord-014597-66vd2mdu	1015	2	latter	latter	JJ
cord-014597-66vd2mdu	1015	3	reaction	reaction	NN
cord-014597-66vd2mdu	1015	4	produced	produce	VBD
cord-014597-66vd2mdu	1015	5	equimolar	equimolar	JJ
cord-014597-66vd2mdu	1015	6	quantities	quantity	NNS
cord-014597-66vd2mdu	1015	7	of	of	IN
cord-014597-66vd2mdu	1015	8	monosialylated	monosialylate	VBN
cord-014597-66vd2mdu	1015	9	and	and	CC
cord-014597-66vd2mdu	1015	10	disialylated	disialylate	VBN
cord-014597-66vd2mdu	1015	11	Cetuximab	Cetuximab	NNP
cord-014597-66vd2mdu	1015	12	and	and	CC
cord-014597-66vd2mdu	1015	13	polyclonal	polyclonal	JJ
cord-014597-66vd2mdu	1015	14	antibodies	antibody	NNS
cord-014597-66vd2mdu	1015	15	.	.	.
cord-014597-66vd2mdu	1016	1	The	the	DT
cord-014597-66vd2mdu	1016	2	results	result	NNS
cord-014597-66vd2mdu	1016	3	suggest	suggest	VBP
cord-014597-66vd2mdu	1016	4	that	that	IN
cord-014597-66vd2mdu	1016	5	for	for	IN
cord-014597-66vd2mdu	1016	6	human	human	JJ
cord-014597-66vd2mdu	1016	7	antibodies	antibody	NNS
cord-014597-66vd2mdu	1016	8	(	(	-LRB-
cord-014597-66vd2mdu	1016	9	150	150	CD
cord-014597-66vd2mdu	1016	10	kDa	kDa	NNPS
cord-014597-66vd2mdu	1016	11	)	)	-RRB-
cord-014597-66vd2mdu	1016	12	there	there	EX
cord-014597-66vd2mdu	1016	13	may	may	MD
cord-014597-66vd2mdu	1016	14	be	be	VB
cord-014597-66vd2mdu	1016	15	a	a	DT
cord-014597-66vd2mdu	1016	16	limitation	limitation	NN
cord-014597-66vd2mdu	1016	17	for	for	IN
cord-014597-66vd2mdu	1016	18	sialylation	sialylation	NN
cord-014597-66vd2mdu	1016	19	given	give	VBN
cord-014597-66vd2mdu	1016	20	the	the	DT
cord-014597-66vd2mdu	1016	21	steric	steric	JJ
cord-014597-66vd2mdu	1016	22	constraints	constraint	NNS
cord-014597-66vd2mdu	1016	23	between	between	IN
cord-014597-66vd2mdu	1016	24	the	the	DT
cord-014597-66vd2mdu	1016	25	two	two	CD
cord-014597-66vd2mdu	1016	26	CH2	CH2	NNP
cord-014597-66vd2mdu	1016	27	domains	domain	NNS
cord-014597-66vd2mdu	1016	28	of	of	IN
cord-014597-66vd2mdu	1016	29	the	the	DT
cord-014597-66vd2mdu	1016	30	dimeric	dimeric	JJ
cord-014597-66vd2mdu	1016	31	structure	structure	NN
cord-014597-66vd2mdu	1016	32	.	.	.
cord-014597-66vd2mdu	1017	1	The	the	DT
cord-014597-66vd2mdu	1017	2	ability	ability	NN
cord-014597-66vd2mdu	1017	3	to	to	TO
cord-014597-66vd2mdu	1017	4	sialylate	sialylate	VB
cord-014597-66vd2mdu	1017	5	the	the	DT
cord-014597-66vd2mdu	1017	6	smaller	small	JJR
cord-014597-66vd2mdu	1017	7	camelid	camelid	NNP
cord-014597-66vd2mdu	1017	8	antibody	antibody	NN
cord-014597-66vd2mdu	1017	9	(	(	-LRB-
cord-014597-66vd2mdu	1017	10	80	80	CD
cord-014597-66vd2mdu	1017	11	kDa	kDa	NNS
cord-014597-66vd2mdu	1017	12	)	)	-RRB-
cord-014597-66vd2mdu	1017	13	was	be	VBD
cord-014597-66vd2mdu	1017	14	greater	great	JJR
cord-014597-66vd2mdu	1017	15	resulting	result	VBG
cord-014597-66vd2mdu	1017	16	in	in	IN
cord-014597-66vd2mdu	1017	17	a	a	DT
cord-014597-66vd2mdu	1017	18	high	high	JJ
cord-014597-66vd2mdu	1017	19	(	(	-LRB-
cord-014597-66vd2mdu	1017	20	>	>	NFP
cord-014597-66vd2mdu	1017	21	90	90	CD
cord-014597-66vd2mdu	1017	22	%	%	NN
cord-014597-66vd2mdu	1017	23	)	)	-RRB-
cord-014597-66vd2mdu	1017	24	level	level	NN
cord-014597-66vd2mdu	1017	25	of	of	IN
cord-014597-66vd2mdu	1017	26	disialylated	disialylated	JJ
cord-014597-66vd2mdu	1017	27	glycans	glycan	NNS
cord-014597-66vd2mdu	1017	28	.	.	.
cord-014597-66vd2mdu	1018	1	This	this	DT
cord-014597-66vd2mdu	1018	2	suggests	suggest	VBZ
cord-014597-66vd2mdu	1018	3	that	that	IN
cord-014597-66vd2mdu	1018	4	the	the	DT
cord-014597-66vd2mdu	1018	5	steric	steric	JJ
cord-014597-66vd2mdu	1018	6	constraints	constraint	NNS
cord-014597-66vd2mdu	1018	7	for	for	IN
cord-014597-66vd2mdu	1018	8	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1018	9	may	may	MD
cord-014597-66vd2mdu	1018	10	be	be	VB
cord-014597-66vd2mdu	1018	11	lower	low	JJR
cord-014597-66vd2mdu	1018	12	.	.	.
cord-014597-66vd2mdu	1019	1	These	these	DT
cord-014597-66vd2mdu	1019	2	sialylated	sialylate	VBN
cord-014597-66vd2mdu	1019	3	antibodies	antibody	NNS
cord-014597-66vd2mdu	1019	4	have	have	VBP
cord-014597-66vd2mdu	1019	5	significant	significant	JJ
cord-014597-66vd2mdu	1019	6	potential	potential	JJ
cord-014597-66vd2mdu	1019	7	clinical	clinical	JJ
cord-014597-66vd2mdu	1019	8	importance	importance	NN
cord-014597-66vd2mdu	1019	9	for	for	IN
cord-014597-66vd2mdu	1019	10	their	-PRON-	PRP$
cord-014597-66vd2mdu	1019	11	ant	ant	JJ
cord-014597-66vd2mdu	1019	12	-	-	HYPH
cord-014597-66vd2mdu	1019	13	inflammatory	inflammatory	JJ
cord-014597-66vd2mdu	1019	14	activities	activity	NNS
cord-014597-66vd2mdu	1019	15	.	.	.
cord-014597-66vd2mdu	1020	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1020	2	have	have	VBP
cord-014597-66vd2mdu	1020	3	modified	modify	VBN
cord-014597-66vd2mdu	1020	4	the	the	DT
cord-014597-66vd2mdu	1020	5	glycans	glycan	NNS
cord-014597-66vd2mdu	1020	6	of	of	IN
cord-014597-66vd2mdu	1020	7	antibodies	antibody	NNS
cord-014597-66vd2mdu	1020	8	following	follow	VBG
cord-014597-66vd2mdu	1020	9	immobilization	immobilization	NN
cord-014597-66vd2mdu	1020	10	on	on	IN
cord-014597-66vd2mdu	1020	11	an	an	DT
cord-014597-66vd2mdu	1020	12	affinity	affinity	NN
cord-014597-66vd2mdu	1020	13	ligand	ligand	NN
cord-014597-66vd2mdu	1020	14	column	column	NN
cord-014597-66vd2mdu	1020	15	.	.	.
cord-014597-66vd2mdu	1021	1	This	this	DT
cord-014597-66vd2mdu	1021	2	allows	allow	VBZ
cord-014597-66vd2mdu	1021	3	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	1021	4	transformation	transformation	NN
cord-014597-66vd2mdu	1021	5	in	in	IN
cord-014597-66vd2mdu	1021	6	a	a	DT
cord-014597-66vd2mdu	1021	7	solid	solid	JJ
cord-014597-66vd2mdu	1021	8	state	state	NN
cord-014597-66vd2mdu	1021	9	that	that	WDT
cord-014597-66vd2mdu	1021	10	has	have	VBZ
cord-014597-66vd2mdu	1021	11	a	a	DT
cord-014597-66vd2mdu	1021	12	distinct	distinct	JJ
cord-014597-66vd2mdu	1021	13	advantage	advantage	NN
cord-014597-66vd2mdu	1021	14	over	over	IN
cord-014597-66vd2mdu	1021	15	the	the	DT
cord-014597-66vd2mdu	1021	16	equivalent	equivalent	JJ
cord-014597-66vd2mdu	1021	17	transformation	transformation	NN
cord-014597-66vd2mdu	1021	18	in	in	IN
cord-014597-66vd2mdu	1021	19	solution	solution	NN
cord-014597-66vd2mdu	1021	20	because	because	IN
cord-014597-66vd2mdu	1021	21	the	the	DT
cord-014597-66vd2mdu	1021	22	enzymes	enzyme	NNS
cord-014597-66vd2mdu	1021	23	and	and	CC
cord-014597-66vd2mdu	1021	24	buffers	buffer	NNS
cord-014597-66vd2mdu	1021	25	can	can	MD
cord-014597-66vd2mdu	1021	26	be	be	VB
cord-014597-66vd2mdu	1021	27	washed	wash	VBN
cord-014597-66vd2mdu	1021	28	out	out	RP
cord-014597-66vd2mdu	1021	29	on	on	IN
cord-014597-66vd2mdu	1021	30	completion	completion	NN
cord-014597-66vd2mdu	1021	31	of	of	IN
cord-014597-66vd2mdu	1021	32	the	the	DT
cord-014597-66vd2mdu	1021	33	modification	modification	NN
cord-014597-66vd2mdu	1021	34	leaving	leave	VBG
cord-014597-66vd2mdu	1021	35	the	the	DT
cord-014597-66vd2mdu	1021	36	antibody	antibody	NN
cord-014597-66vd2mdu	1021	37	still	still	RB
cord-014597-66vd2mdu	1021	38	attached	attach	VBN
cord-014597-66vd2mdu	1021	39	to	to	IN
cord-014597-66vd2mdu	1021	40	the	the	DT
cord-014597-66vd2mdu	1021	41	affinity	affinity	NN
cord-014597-66vd2mdu	1021	42	ligand	ligand	NN
cord-014597-66vd2mdu	1021	43	.	.	.
cord-014597-66vd2mdu	1022	1	This	this	DT
cord-014597-66vd2mdu	1022	2	enables	enable	VBZ
cord-014597-66vd2mdu	1022	3	repeated	repeat	VBD
cord-014597-66vd2mdu	1022	4	rounds	round	NNS
cord-014597-66vd2mdu	1022	5	of	of	IN
cord-014597-66vd2mdu	1022	6	an	an	DT
cord-014597-66vd2mdu	1022	7	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	1022	8	reaction	reaction	NN
cord-014597-66vd2mdu	1022	9	or	or	CC
cord-014597-66vd2mdu	1022	10	sequential	sequential	JJ
cord-014597-66vd2mdu	1022	11	reaction	reaction	NN
cord-014597-66vd2mdu	1022	12	steps	step	NNS
cord-014597-66vd2mdu	1022	13	without	without	IN
cord-014597-66vd2mdu	1022	14	the	the	DT
cord-014597-66vd2mdu	1022	15	need	need	NN
cord-014597-66vd2mdu	1022	16	for	for	IN
cord-014597-66vd2mdu	1022	17	intermediate	intermediate	JJ
cord-014597-66vd2mdu	1022	18	antibody	antibody	NN
cord-014597-66vd2mdu	1022	19	purification	purification	NN
cord-014597-66vd2mdu	1022	20	.	.	.
cord-014597-66vd2mdu	1023	1	The	the	DT
cord-014597-66vd2mdu	1023	2	antibody	antibody	NN
cord-014597-66vd2mdu	1023	3	can	can	MD
cord-014597-66vd2mdu	1023	4	be	be	VB
cord-014597-66vd2mdu	1023	5	removed	remove	VBN
cord-014597-66vd2mdu	1023	6	eventually	eventually	RB
cord-014597-66vd2mdu	1023	7	from	from	IN
cord-014597-66vd2mdu	1023	8	the	the	DT
cord-014597-66vd2mdu	1023	9	column	column	NN
cord-014597-66vd2mdu	1023	10	by	by	IN
cord-014597-66vd2mdu	1023	11	application	application	NN
cord-014597-66vd2mdu	1023	12	of	of	IN
cord-014597-66vd2mdu	1023	13	an	an	DT
cord-014597-66vd2mdu	1023	14	elution	elution	NN
cord-014597-66vd2mdu	1023	15	buffer	buffer	NN
cord-014597-66vd2mdu	1023	16	once	once	RB
cord-014597-66vd2mdu	1023	17	all	all	DT
cord-014597-66vd2mdu	1023	18	desired	desire	VBN
cord-014597-66vd2mdu	1023	19	glycan	glycan	NN
cord-014597-66vd2mdu	1023	20	modification	modification	NN
cord-014597-66vd2mdu	1023	21	have	have	VBP
cord-014597-66vd2mdu	1023	22	been	be	VBN
cord-014597-66vd2mdu	1023	23	made	make	VBN
cord-014597-66vd2mdu	1023	24	.	.	.
cord-014597-66vd2mdu	1024	1	Since	since	IN
cord-014597-66vd2mdu	1024	2	affinity	affinity	NN
cord-014597-66vd2mdu	1024	3	ligand	ligand	NN
cord-014597-66vd2mdu	1024	4	purification	purification	NN
cord-014597-66vd2mdu	1024	5	of	of	IN
cord-014597-66vd2mdu	1024	6	antibodies	antibody	NNS
cord-014597-66vd2mdu	1024	7	is	be	VBZ
cord-014597-66vd2mdu	1024	8	performed	perform	VBN
cord-014597-66vd2mdu	1024	9	routinely	routinely	RB
cord-014597-66vd2mdu	1024	10	as	as	IN
cord-014597-66vd2mdu	1024	11	an	an	DT
cord-014597-66vd2mdu	1024	12	initial	initial	JJ
cord-014597-66vd2mdu	1024	13	step	step	NN
cord-014597-66vd2mdu	1024	14	of	of	IN
cord-014597-66vd2mdu	1024	15	purification	purification	NN
cord-014597-66vd2mdu	1024	16	after	after	IN
cord-014597-66vd2mdu	1024	17	cell	cell	NN
cord-014597-66vd2mdu	1024	18	culture	culture	NN
cord-014597-66vd2mdu	1024	19	,	,	,
cord-014597-66vd2mdu	1024	20	the	the	DT
cord-014597-66vd2mdu	1024	21	glycan	glycan	NN
cord-014597-66vd2mdu	1024	22	modification	modification	NN
cord-014597-66vd2mdu	1024	23	can	can	MD
cord-014597-66vd2mdu	1024	24	easily	easily	RB
cord-014597-66vd2mdu	1024	25	be	be	VB
cord-014597-66vd2mdu	1024	26	incorporated	incorporate	VBN
cord-014597-66vd2mdu	1024	27	into	into	IN
cord-014597-66vd2mdu	1024	28	this	this	DT
cord-014597-66vd2mdu	1024	29	process	process	NN
cord-014597-66vd2mdu	1024	30	.	.	.
cord-014597-66vd2mdu	1025	1	The	the	DT
cord-014597-66vd2mdu	1025	2	enrichment	enrichment	NN
cord-014597-66vd2mdu	1025	3	of	of	IN
cord-014597-66vd2mdu	1025	4	the	the	DT
cord-014597-66vd2mdu	1025	5	resulting	result	VBG
cord-014597-66vd2mdu	1025	6	antibody	antibody	NN
cord-014597-66vd2mdu	1025	7	for	for	IN
cord-014597-66vd2mdu	1025	8	a	a	DT
cord-014597-66vd2mdu	1025	9	targeted	target	VBN
cord-014597-66vd2mdu	1025	10	glycoform	glycoform	NN
cord-014597-66vd2mdu	1025	11	can	can	MD
cord-014597-66vd2mdu	1025	12	enhance	enhance	VB
cord-014597-66vd2mdu	1025	13	the	the	DT
cord-014597-66vd2mdu	1025	14	potential	potential	JJ
cord-014597-66vd2mdu	1025	15	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1025	16	efficacy	efficacy	NN
cord-014597-66vd2mdu	1025	17	as	as	IN
cord-014597-66vd2mdu	1025	18	it	-PRON-	PRP
cord-014597-66vd2mdu	1025	19	is	be	VBZ
cord-014597-66vd2mdu	1025	20	known	know	VBN
cord-014597-66vd2mdu	1025	21	that	that	IN
cord-014597-66vd2mdu	1025	22	specific	specific	JJ
cord-014597-66vd2mdu	1025	23	glycoforms	glycoform	NNS
cord-014597-66vd2mdu	1025	24	are	be	VBP
cord-014597-66vd2mdu	1025	25	required	require	VBN
cord-014597-66vd2mdu	1025	26	for	for	IN
cord-014597-66vd2mdu	1025	27	certain	certain	JJ
cord-014597-66vd2mdu	1025	28	biological	biological	JJ
cord-014597-66vd2mdu	1025	29	effects	effect	NNS
cord-014597-66vd2mdu	1025	30	.	.	.
cord-014597-66vd2mdu	1026	1	[	[	-LRB-
cord-014597-66vd2mdu	1026	2	1	1	CD
cord-014597-66vd2mdu	1026	3	,	,	,
cord-014597-66vd2mdu	1026	4	2	2	CD
cord-014597-66vd2mdu	1026	5	]	]	-RRB-
cord-014597-66vd2mdu	1026	6	.	.	.
cord-014597-66vd2mdu	1027	1	This	this	DT
cord-014597-66vd2mdu	1027	2	is	be	VBZ
cord-014597-66vd2mdu	1027	3	mainly	mainly	RB
cord-014597-66vd2mdu	1027	4	because	because	IN
cord-014597-66vd2mdu	1027	5	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1027	6	can	can	MD
cord-014597-66vd2mdu	1027	7	be	be	VB
cord-014597-66vd2mdu	1027	8	enriched	enrich	VBN
cord-014597-66vd2mdu	1027	9	/	/	SYM
cord-014597-66vd2mdu	1027	10	deprived	deprive	VBN
cord-014597-66vd2mdu	1027	11	for	for	IN
cord-014597-66vd2mdu	1027	12	specific	specific	JJ
cord-014597-66vd2mdu	1027	13	proteins	protein	NNS
cord-014597-66vd2mdu	1027	14	,	,	,
cord-014597-66vd2mdu	1027	15	based	base	VBN
cord-014597-66vd2mdu	1027	16	on	on	IN
cord-014597-66vd2mdu	1027	17	their	-PRON-	PRP$
cord-014597-66vd2mdu	1027	18	functional	functional	JJ
cord-014597-66vd2mdu	1027	19	purpose	purpose	NN
cord-014597-66vd2mdu	1027	20	and	and	CC
cord-014597-66vd2mdu	1027	21	their	-PRON-	PRP$
cord-014597-66vd2mdu	1027	22	cellular	cellular	JJ
cord-014597-66vd2mdu	1027	23	origin	origin	NN
cord-014597-66vd2mdu	1027	24	.	.	.
cord-014597-66vd2mdu	1028	1	Recently	recently	RB
cord-014597-66vd2mdu	1028	2	,	,	,
cord-014597-66vd2mdu	1028	3	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1028	4	purified	purify	VBN
cord-014597-66vd2mdu	1028	5	from	from	IN
cord-014597-66vd2mdu	1028	6	the	the	DT
cord-014597-66vd2mdu	1028	7	supernatant	supernatant	NN
cord-014597-66vd2mdu	1028	8	of	of	IN
cord-014597-66vd2mdu	1028	9	T24	T24	NNP
cord-014597-66vd2mdu	1028	10	bladder	bladder	NN
cord-014597-66vd2mdu	1028	11	cancer	cancer	NN
cord-014597-66vd2mdu	1028	12	cells	cell	NNS
cord-014597-66vd2mdu	1028	13	were	be	VBD
cord-014597-66vd2mdu	1028	14	reported	report	VBN
cord-014597-66vd2mdu	1028	15	to	to	TO
cord-014597-66vd2mdu	1028	16	be	be	VB
cord-014597-66vd2mdu	1028	17	enriched	enrich	VBN
cord-014597-66vd2mdu	1028	18	for	for	IN
cord-014597-66vd2mdu	1028	19	Bcl-2	Bcl-2	NNP
cord-014597-66vd2mdu	1028	20	and	and	CC
cord-014597-66vd2mdu	1028	21	cyclin	cyclin	NN
cord-014597-66vd2mdu	1028	22	D1	d1	NN
cord-014597-66vd2mdu	1028	23	(	(	-LRB-
cord-014597-66vd2mdu	1028	24	anti	anti	JJ
cord-014597-66vd2mdu	1028	25	-	-	JJ
cord-014597-66vd2mdu	1028	26	apoptotic	apoptotic	JJ
cord-014597-66vd2mdu	1028	27	proteins	protein	NNS
cord-014597-66vd2mdu	1028	28	)	)	-RRB-
cord-014597-66vd2mdu	1028	29	,	,	,
cord-014597-66vd2mdu	1028	30	but	but	CC
cord-014597-66vd2mdu	1028	31	deprived	deprive	VBN
cord-014597-66vd2mdu	1028	32	for	for	IN
cord-014597-66vd2mdu	1028	33	Bax	Bax	NNP
cord-014597-66vd2mdu	1028	34	and	and	CC
cord-014597-66vd2mdu	1028	35	caspase-3	caspase-3	NNP
cord-014597-66vd2mdu	1028	36	proteins	protein	NNS
cord-014597-66vd2mdu	1028	37	(	(	-LRB-
cord-014597-66vd2mdu	1028	38	pro	pro	JJ
cord-014597-66vd2mdu	1028	39	-	-	JJ
cord-014597-66vd2mdu	1028	40	apoptotic	apoptotic	JJ
cord-014597-66vd2mdu	1028	41	proteins	protein	NNS
cord-014597-66vd2mdu	1028	42	)	)	-RRB-
cord-014597-66vd2mdu	1028	43	contributing	contribute	VBG
cord-014597-66vd2mdu	1028	44	towards	towards	IN
cord-014597-66vd2mdu	1028	45	immunity	immunity	NN
cord-014597-66vd2mdu	1028	46	against	against	IN
cord-014597-66vd2mdu	1028	47	programmed	program	VBN
cord-014597-66vd2mdu	1028	48	cell	cell	NN
cord-014597-66vd2mdu	1028	49	-	-	HYPH
cord-014597-66vd2mdu	1028	50	death	death	NN
cord-014597-66vd2mdu	1028	51	[	[	-LRB-
cord-014597-66vd2mdu	1028	52	2	2	CD
cord-014597-66vd2mdu	1028	53	]	]	-RRB-
cord-014597-66vd2mdu	1028	54	.	.	.
cord-014597-66vd2mdu	1029	1	However	however	RB
cord-014597-66vd2mdu	1029	2	,	,	,
cord-014597-66vd2mdu	1029	3	impact	impact	NN
cord-014597-66vd2mdu	1029	4	of	of	IN
cord-014597-66vd2mdu	1029	5	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1029	6	on	on	IN
cord-014597-66vd2mdu	1029	7	CHO	CHO	NNP
cord-014597-66vd2mdu	1029	8	-	-	HYPH
cord-014597-66vd2mdu	1029	9	based	base	VBN
cord-014597-66vd2mdu	1029	10	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	1029	11	has	have	VBZ
cord-014597-66vd2mdu	1029	12	not	not	RB
cord-014597-66vd2mdu	1029	13	been	be	VBN
cord-014597-66vd2mdu	1029	14	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1029	15	yet	yet	RB
cord-014597-66vd2mdu	1029	16	.	.	.
cord-014597-66vd2mdu	1030	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1030	2	,	,	,
cord-014597-66vd2mdu	1030	3	in	in	IN
cord-014597-66vd2mdu	1030	4	this	this	DT
cord-014597-66vd2mdu	1030	5	investigation	investigation	NN
cord-014597-66vd2mdu	1030	6	,	,	,
cord-014597-66vd2mdu	1030	7	we	-PRON-	PRP
cord-014597-66vd2mdu	1030	8	aimed	aim	VBD
cord-014597-66vd2mdu	1030	9	to	to	TO
cord-014597-66vd2mdu	1030	10	evaluate	evaluate	VB
cord-014597-66vd2mdu	1030	11	their	-PRON-	PRP$
cord-014597-66vd2mdu	1030	12	impact	impact	NN
cord-014597-66vd2mdu	1030	13	on	on	IN
cord-014597-66vd2mdu	1030	14	cell	cell	NN
cord-014597-66vd2mdu	1030	15	growth	growth	NN
cord-014597-66vd2mdu	1030	16	and	and	CC
cord-014597-66vd2mdu	1030	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1030	18	protein	protein	NN
cord-014597-66vd2mdu	1030	19	production	production	NN
cord-014597-66vd2mdu	1030	20	from	from	IN
cord-014597-66vd2mdu	1030	21	CHO	CHO	NNP
cord-014597-66vd2mdu	1030	22	cells	cell	NNS
cord-014597-66vd2mdu	1030	23	.	.	.
cord-014597-66vd2mdu	1031	1	Materials	material	NNS
cord-014597-66vd2mdu	1031	2	and	and	CC
cord-014597-66vd2mdu	1031	3	methods	method	NNS
cord-014597-66vd2mdu	1031	4	CHO	CHO	NNP
cord-014597-66vd2mdu	1031	5	-	-	HYPH
cord-014597-66vd2mdu	1031	6	K1	K1	NNP
cord-014597-66vd2mdu	1031	7	cells	cell	NNS
cord-014597-66vd2mdu	1031	8	were	be	VBD
cord-014597-66vd2mdu	1031	9	grown	grow	VBN
cord-014597-66vd2mdu	1031	10	in	in	IN
cord-014597-66vd2mdu	1031	11	chemically	chemically	RB
cord-014597-66vd2mdu	1031	12	-	-	HYPH
cord-014597-66vd2mdu	1031	13	defined	define	VBN
cord-014597-66vd2mdu	1031	14	protein	protein	NN
cord-014597-66vd2mdu	1031	15	-	-	HYPH
cord-014597-66vd2mdu	1031	16	free	free	JJ
cord-014597-66vd2mdu	1031	17	culture	culture	NN
cord-014597-66vd2mdu	1031	18	medium	medium	NN
cord-014597-66vd2mdu	1031	19	(	(	-LRB-
cord-014597-66vd2mdu	1031	20	Life	Life	NNP
cord-014597-66vd2mdu	1031	21	Technologies-1835273	Technologies-1835273	NNP
cord-014597-66vd2mdu	1031	22	)	)	-RRB-
cord-014597-66vd2mdu	1031	23	in	in	IN
cord-014597-66vd2mdu	1031	24	shake	shake	NN
cord-014597-66vd2mdu	1031	25	flask	flask	NN
cord-014597-66vd2mdu	1031	26	(	(	-LRB-
cord-014597-66vd2mdu	1031	27	GX-00125P	GX-00125P	NNP
cord-014597-66vd2mdu	1031	28	)	)	-RRB-
cord-014597-66vd2mdu	1031	29	.	.	.
cord-014597-66vd2mdu	1032	1	The	the	DT
cord-014597-66vd2mdu	1032	2	different	different	JJ
cord-014597-66vd2mdu	1032	3	fractions	fraction	NNS
cord-014597-66vd2mdu	1032	4	of	of	IN
cord-014597-66vd2mdu	1032	5	spent	spend	VBN
cord-014597-66vd2mdu	1032	6	-	-	HYPH
cord-014597-66vd2mdu	1032	7	media	medium	NNS
cord-014597-66vd2mdu	1032	8	(	(	-LRB-
cord-014597-66vd2mdu	1032	9	microvesicles	microvesicles	NNP
cord-014597-66vd2mdu	1032	10	and	and	CC
cord-014597-66vd2mdu	1032	11	microvesicle	microvesicle	NNP
cord-014597-66vd2mdu	1032	12	-	-	HYPH
cord-014597-66vd2mdu	1032	13	free	free	JJ
cord-014597-66vd2mdu	1032	14	spent	spend	VBN
cord-014597-66vd2mdu	1032	15	media	medium	NNS
cord-014597-66vd2mdu	1032	16	)	)	-RRB-
cord-014597-66vd2mdu	1032	17	were	be	VBD
cord-014597-66vd2mdu	1032	18	collected	collect	VBN
cord-014597-66vd2mdu	1032	19	using	use	VBG
cord-014597-66vd2mdu	1032	20	ultracentrifugation	ultracentrifugation	NN
cord-014597-66vd2mdu	1032	21	method	method	NN
cord-014597-66vd2mdu	1032	22	[	[	-LRB-
cord-014597-66vd2mdu	1032	23	1	1	CD
cord-014597-66vd2mdu	1032	24	,	,	,
cord-014597-66vd2mdu	1032	25	3	3	CD
cord-014597-66vd2mdu	1032	26	]	]	-RRB-
cord-014597-66vd2mdu	1032	27	.	.	.
cord-014597-66vd2mdu	1033	1	Quality	quality	NN
cord-014597-66vd2mdu	1033	2	of	of	IN
cord-014597-66vd2mdu	1033	3	different	different	JJ
cord-014597-66vd2mdu	1033	4	fractions	fraction	NNS
cord-014597-66vd2mdu	1033	5	was	be	VBD
cord-014597-66vd2mdu	1033	6	ensured	ensure	VBN
cord-014597-66vd2mdu	1033	7	using	use	VBG
cord-014597-66vd2mdu	1033	8	Western	western	JJ
cord-014597-66vd2mdu	1033	9	blotting	blotting	NN
cord-014597-66vd2mdu	1033	10	for	for	IN
cord-014597-66vd2mdu	1033	11	exosomal	exosomal	JJ
cord-014597-66vd2mdu	1033	12	marker	marker	NN
cord-014597-66vd2mdu	1033	13	,	,	,
cord-014597-66vd2mdu	1033	14	CD63	CD63	NNP
cord-014597-66vd2mdu	1033	15	(	(	-LRB-
cord-014597-66vd2mdu	1033	16	SC-15363	SC-15363	NNP
cord-014597-66vd2mdu	1033	17	)	)	-RRB-
cord-014597-66vd2mdu	1033	18	and	and	CC
cord-014597-66vd2mdu	1033	19	coomassie	coomassie	VB
cord-014597-66vd2mdu	1033	20	stained	stain	VBN
cord-014597-66vd2mdu	1033	21	gel	gel	NN
cord-014597-66vd2mdu	1033	22	for	for	IN
cord-014597-66vd2mdu	1033	23	loading	loading	NN
cord-014597-66vd2mdu	1033	24	control	control	NN
cord-014597-66vd2mdu	1033	25	(	(	-LRB-
cord-014597-66vd2mdu	1033	26	Fig	Fig	NNP
cord-014597-66vd2mdu	1033	27	.	.	.
cord-014597-66vd2mdu	1033	28	1a	1a	CD
cord-014597-66vd2mdu	1033	29	)	)	-RRB-
cord-014597-66vd2mdu	1033	30	.	.	.
cord-014597-66vd2mdu	1034	1	To	to	TO
cord-014597-66vd2mdu	1034	2	evaluate	evaluate	VB
cord-014597-66vd2mdu	1034	3	impact	impact	NN
cord-014597-66vd2mdu	1034	4	on	on	IN
cord-014597-66vd2mdu	1034	5	cell	cell	NN
cord-014597-66vd2mdu	1034	6	growth	growth	NN
cord-014597-66vd2mdu	1034	7	,	,	,
cord-014597-66vd2mdu	1034	8	cells	cell	NNS
cord-014597-66vd2mdu	1034	9	were	be	VBD
cord-014597-66vd2mdu	1034	10	seeded	seed	VBN
cord-014597-66vd2mdu	1034	11	with	with	IN
cord-014597-66vd2mdu	1034	12	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1034	13	and	and	CC
cord-014597-66vd2mdu	1034	14	microvesicle	microvesicle	NNP
cord-014597-66vd2mdu	1034	15	-	-	HYPH
cord-014597-66vd2mdu	1034	16	free	free	JJ
cord-014597-66vd2mdu	1034	17	fraction	fraction	NN
cord-014597-66vd2mdu	1034	18	collected	collect	VBN
cord-014597-66vd2mdu	1034	19	from	from	IN
cord-014597-66vd2mdu	1034	20	log	log	NN
cord-014597-66vd2mdu	1034	21	-	-	HYPH
cord-014597-66vd2mdu	1034	22	phase	phase	NN
cord-014597-66vd2mdu	1034	23	of	of	IN
cord-014597-66vd2mdu	1034	24	culture	culture	NN
cord-014597-66vd2mdu	1034	25	and	and	CC
cord-014597-66vd2mdu	1034	26	cell	cell	NN
cord-014597-66vd2mdu	1034	27	counts	count	NNS
cord-014597-66vd2mdu	1034	28	were	be	VBD
cord-014597-66vd2mdu	1034	29	performed	perform	VBN
cord-014597-66vd2mdu	1034	30	by	by	IN
cord-014597-66vd2mdu	1034	31	ViCell	vicell	NN
cord-014597-66vd2mdu	1034	32	using	use	VBG
cord-014597-66vd2mdu	1034	33	trypan	trypan	NNP
cord-014597-66vd2mdu	1034	34	-	-	HYPH
cord-014597-66vd2mdu	1034	35	blue	blue	JJ
cord-014597-66vd2mdu	1034	36	dye	dye	NN
cord-014597-66vd2mdu	1034	37	exclusion	exclusion	NN
cord-014597-66vd2mdu	1034	38	method	method	NN
cord-014597-66vd2mdu	1034	39	.	.	.
cord-014597-66vd2mdu	1035	1	For	for	IN
cord-014597-66vd2mdu	1035	2	impact	impact	NN
cord-014597-66vd2mdu	1035	3	on	on	IN
cord-014597-66vd2mdu	1035	4	productivity	productivity	NN
cord-014597-66vd2mdu	1035	5	,	,	,
cord-014597-66vd2mdu	1035	6	cell	cell	NN
cord-014597-66vd2mdu	1035	7	-	-	HYPH
cord-014597-66vd2mdu	1035	8	free	free	JJ
cord-014597-66vd2mdu	1035	9	supernatant	supernatant	NN
cord-014597-66vd2mdu	1035	10	,	,	,
cord-014597-66vd2mdu	1035	11	collected	collect	VBN
cord-014597-66vd2mdu	1035	12	from	from	IN
cord-014597-66vd2mdu	1035	13	microvesicle	microvesicle	NNP
cord-014597-66vd2mdu	1035	14	-	-	HYPH
cord-014597-66vd2mdu	1035	15	treated	treat	VBN
cord-014597-66vd2mdu	1035	16	human	human	JJ
cord-014597-66vd2mdu	1035	17	IgG	igg	NN
cord-014597-66vd2mdu	1035	18	secreting	secrete	VBG
cord-014597-66vd2mdu	1035	19	CHO	CHO	NNP
cord-014597-66vd2mdu	1035	20	culture	culture	NN
cord-014597-66vd2mdu	1035	21	from	from	IN
cord-014597-66vd2mdu	1035	22	stationary	stationary	JJ
cord-014597-66vd2mdu	1035	23	-	-	HYPH
cord-014597-66vd2mdu	1035	24	phase	phase	NN
cord-014597-66vd2mdu	1035	25	of	of	IN
cord-014597-66vd2mdu	1035	26	culture	culture	NN
cord-014597-66vd2mdu	1035	27	with	with	IN
cord-014597-66vd2mdu	1035	28	respective	respective	JJ
cord-014597-66vd2mdu	1035	29	control	control	NN
cord-014597-66vd2mdu	1035	30	,	,	,
cord-014597-66vd2mdu	1035	31	was	be	VBD
cord-014597-66vd2mdu	1035	32	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1035	33	using	use	VBG
cord-014597-66vd2mdu	1035	34	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1035	35	(	(	-LRB-
cord-014597-66vd2mdu	1035	36	ab100547	ab100547	NNP
cord-014597-66vd2mdu	1035	37	)	)	-RRB-
cord-014597-66vd2mdu	1035	38	.	.	.
cord-014597-66vd2mdu	1036	1	Microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1036	2	collected	collect	VBN
cord-014597-66vd2mdu	1036	3	from	from	IN
cord-014597-66vd2mdu	1036	4	10	10	CD
cord-014597-66vd2mdu	1036	5	%	%	NN
cord-014597-66vd2mdu	1036	6	of	of	IN
cord-014597-66vd2mdu	1036	7	media	medium	NNS
cord-014597-66vd2mdu	1036	8	(	(	-LRB-
cord-014597-66vd2mdu	1036	9	by	by	IN
cord-014597-66vd2mdu	1036	10	volume	volume	NN
cord-014597-66vd2mdu	1036	11	)	)	-RRB-
cord-014597-66vd2mdu	1036	12	from	from	IN
cord-014597-66vd2mdu	1036	13	routine	routine	JJ
cord-014597-66vd2mdu	1036	14	maintenance	maintenance	NN
cord-014597-66vd2mdu	1036	15	cultures	culture	NNS
cord-014597-66vd2mdu	1036	16	compared	compare	VBN
cord-014597-66vd2mdu	1036	17	to	to	IN
cord-014597-66vd2mdu	1036	18	working	work	VBG
cord-014597-66vd2mdu	1036	19	volume	volume	NN
cord-014597-66vd2mdu	1036	20	for	for	IN
cord-014597-66vd2mdu	1036	21	microvesiclesupplementation	microvesiclesupplementation	NN
cord-014597-66vd2mdu	1036	22	were	be	VBD
cord-014597-66vd2mdu	1036	23	used	use	VBN
cord-014597-66vd2mdu	1036	24	in	in	IN
cord-014597-66vd2mdu	1036	25	each	each	DT
cord-014597-66vd2mdu	1036	26	experiment	experiment	NN
cord-014597-66vd2mdu	1036	27	.	.	.
cord-014597-66vd2mdu	1037	1	The	the	DT
cord-014597-66vd2mdu	1037	2	growth	growth	NN
cord-014597-66vd2mdu	1037	3	of	of	IN
cord-014597-66vd2mdu	1037	4	microvesicle	microvesicle	NNP
cord-014597-66vd2mdu	1037	5	-	-	HYPH
cord-014597-66vd2mdu	1037	6	supplemented	supplement	VBN
cord-014597-66vd2mdu	1037	7	cultures	culture	NNS
cord-014597-66vd2mdu	1037	8	had	have	VBD
cord-014597-66vd2mdu	1037	9	shorter	short	JJR
cord-014597-66vd2mdu	1037	10	lag	lag	NN
cord-014597-66vd2mdu	1037	11	-	-	HYPH
cord-014597-66vd2mdu	1037	12	phase	phase	NN
cord-014597-66vd2mdu	1037	13	and	and	CC
cord-014597-66vd2mdu	1037	14	achieved	achieve	VBD
cord-014597-66vd2mdu	1037	15	1.2	1.2	CD
cord-014597-66vd2mdu	1037	16	fold	fold	VB
cord-014597-66vd2mdu	1037	17	higher	high	JJR
cord-014597-66vd2mdu	1037	18	maximum	maximum	JJ
cord-014597-66vd2mdu	1037	19	cell	cell	NN
cord-014597-66vd2mdu	1037	20	density	density	NN
cord-014597-66vd2mdu	1037	21	(	(	-LRB-
cord-014597-66vd2mdu	1037	22	1.46x10	1.46x10	CD
cord-014597-66vd2mdu	1037	23	6	6	CD
cord-014597-66vd2mdu	1037	24	viable	viable	JJ
cord-014597-66vd2mdu	1037	25	cells	cell	NNS
cord-014597-66vd2mdu	1037	26	/	/	SYM
cord-014597-66vd2mdu	1037	27	mL	ml	NN
cord-014597-66vd2mdu	1037	28	)	)	-RRB-
cord-014597-66vd2mdu	1037	29	compared	compare	VBN
cord-014597-66vd2mdu	1037	30	to	to	IN
cord-014597-66vd2mdu	1037	31	untreated	untreated	JJ
cord-014597-66vd2mdu	1037	32	standard	standard	JJ
cord-014597-66vd2mdu	1037	33	culture	culture	NN
cord-014597-66vd2mdu	1037	34	(	(	-LRB-
cord-014597-66vd2mdu	1037	35	1.21	1.21	CD
cord-014597-66vd2mdu	1037	36	x10	x10	CD
cord-014597-66vd2mdu	1037	37	6	6	CD
cord-014597-66vd2mdu	1037	38	viable	viable	JJ
cord-014597-66vd2mdu	1037	39	cells	cell	NNS
cord-014597-66vd2mdu	1037	40	/	/	SYM
cord-014597-66vd2mdu	1037	41	mL	ml	NN
cord-014597-66vd2mdu	1037	42	)	)	-RRB-
cord-014597-66vd2mdu	1037	43	and	and	CC
cord-014597-66vd2mdu	1037	44	maintained	maintain	VBD
cord-014597-66vd2mdu	1037	45	higher	higher	RBR
cord-014597-66vd2mdu	1037	46	for	for	IN
cord-014597-66vd2mdu	1037	47	the	the	DT
cord-014597-66vd2mdu	1037	48	remaining	remain	VBG
cord-014597-66vd2mdu	1037	49	period	period	NN
cord-014597-66vd2mdu	1037	50	of	of	IN
cord-014597-66vd2mdu	1037	51	batch	batch	NN
cord-014597-66vd2mdu	1037	52	culture	culture	NN
cord-014597-66vd2mdu	1038	1	(	(	-LRB-
cord-014597-66vd2mdu	1038	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1038	3	.	.	.
cord-014597-66vd2mdu	1038	4	1b	1b	CD
cord-014597-66vd2mdu	1038	5	)	)	-RRB-
cord-014597-66vd2mdu	1038	6	.	.	.
cord-014597-66vd2mdu	1039	1	However	however	RB
cord-014597-66vd2mdu	1039	2	,	,	,
cord-014597-66vd2mdu	1039	3	microvesicle	microvesicle	NNP
cord-014597-66vd2mdu	1039	4	-	-	HYPH
cord-014597-66vd2mdu	1039	5	free	free	JJ
cord-014597-66vd2mdu	1039	6	fraction	fraction	NN
cord-014597-66vd2mdu	1039	7	did	do	VBD
cord-014597-66vd2mdu	1039	8	not	not	RB
cord-014597-66vd2mdu	1039	9	had	have	VBD
cord-014597-66vd2mdu	1039	10	significant	significant	JJ
cord-014597-66vd2mdu	1039	11	impact	impact	NN
cord-014597-66vd2mdu	1039	12	on	on	IN
cord-014597-66vd2mdu	1039	13	growth	growth	NN
cord-014597-66vd2mdu	1039	14	.	.	.
cord-014597-66vd2mdu	1040	1	The	the	DT
cord-014597-66vd2mdu	1040	2	viability	viability	NN
cord-014597-66vd2mdu	1040	3	of	of	IN
cord-014597-66vd2mdu	1040	4	microvesicle	microvesicle	NN
cord-014597-66vd2mdu	1040	5	-	-	HYPH
cord-014597-66vd2mdu	1040	6	supplemented	supplement	VBN
cord-014597-66vd2mdu	1040	7	cultures	culture	NNS
cord-014597-66vd2mdu	1040	8	,	,	,
cord-014597-66vd2mdu	1040	9	similar	similar	JJ
cord-014597-66vd2mdu	1040	10	to	to	IN
cord-014597-66vd2mdu	1040	11	microvesicle	microvesicle	NNP
cord-014597-66vd2mdu	1040	12	-	-	HYPH
cord-014597-66vd2mdu	1040	13	free	free	JJ
cord-014597-66vd2mdu	1040	14	media	medium	NNS
cord-014597-66vd2mdu	1040	15	supplemented	supplement	VBN
cord-014597-66vd2mdu	1040	16	,	,	,
cord-014597-66vd2mdu	1040	17	was	be	VBD
cord-014597-66vd2mdu	1040	18	also	also	RB
cord-014597-66vd2mdu	1040	19	higher	high	JJR
cord-014597-66vd2mdu	1040	20	compared	compare	VBN
cord-014597-66vd2mdu	1040	21	to	to	IN
cord-014597-66vd2mdu	1040	22	standard	standard	JJ
cord-014597-66vd2mdu	1040	23	culture	culture	NN
cord-014597-66vd2mdu	1040	24	suggesting	suggest	VBG
cord-014597-66vd2mdu	1040	25	potential	potential	JJ
cord-014597-66vd2mdu	1040	26	use	use	NN
cord-014597-66vd2mdu	1040	27	of	of	IN
cord-014597-66vd2mdu	1040	28	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1040	29	for	for	IN
cord-014597-66vd2mdu	1040	30	regulating	regulate	VBG
cord-014597-66vd2mdu	1040	31	CHO	CHO	NNP
cord-014597-66vd2mdu	1040	32	growth	growth	NN
cord-014597-66vd2mdu	1040	33	in	in	IN
cord-014597-66vd2mdu	1040	34	production	production	NN
cord-014597-66vd2mdu	1040	35	cultures	culture	NNS
cord-014597-66vd2mdu	1040	36	.	.	.
cord-014597-66vd2mdu	1041	1	This	this	DT
cord-014597-66vd2mdu	1041	2	could	could	MD
cord-014597-66vd2mdu	1041	3	be	be	VB
cord-014597-66vd2mdu	1041	4	possibily	possibily	RB
cord-014597-66vd2mdu	1041	5	because	because	IN
cord-014597-66vd2mdu	1041	6	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1041	7	have	have	VBP
cord-014597-66vd2mdu	1041	8	already	already	RB
cord-014597-66vd2mdu	1041	9	been	be	VBN
cord-014597-66vd2mdu	1041	10	reported	report	VBN
cord-014597-66vd2mdu	1041	11	to	to	TO
cord-014597-66vd2mdu	1041	12	be	be	VB
cord-014597-66vd2mdu	1041	13	enriched	enrich	VBN
cord-014597-66vd2mdu	1041	14	with	with	IN
cord-014597-66vd2mdu	1041	15	cell	cell	NN
cord-014597-66vd2mdu	1041	16	growth	growth	NN
cord-014597-66vd2mdu	1041	17	/	/	SYM
cord-014597-66vd2mdu	1041	18	death	death	NN
cord-014597-66vd2mdu	1041	19	-	-	HYPH
cord-014597-66vd2mdu	1041	20	regulating	regulate	VBG
cord-014597-66vd2mdu	1041	21	proteins	protein	NNS
cord-014597-66vd2mdu	1041	22	and	and	CC
cord-014597-66vd2mdu	1041	23	hence	hence	RB
cord-014597-66vd2mdu	1041	24	facilitating	facilitate	VBG
cord-014597-66vd2mdu	1041	25	cell	cell	NN
cord-014597-66vd2mdu	1041	26	growth	growth	NN
cord-014597-66vd2mdu	1041	27	[	[	-LRB-
cord-014597-66vd2mdu	1041	28	2	2	CD
cord-014597-66vd2mdu	1041	29	,	,	,
cord-014597-66vd2mdu	1041	30	3	3	CD
cord-014597-66vd2mdu	1041	31	]	]	-RRB-
cord-014597-66vd2mdu	1041	32	.	.	.
cord-014597-66vd2mdu	1042	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1042	2	have	have	VBP
cord-014597-66vd2mdu	1042	3	also	also	RB
cord-014597-66vd2mdu	1042	4	observed	observe	VBN
cord-014597-66vd2mdu	1042	5	abundance	abundance	NN
cord-014597-66vd2mdu	1042	6	of	of	IN
cord-014597-66vd2mdu	1042	7	cell	cell	NN
cord-014597-66vd2mdu	1042	8	cycle	cycle	NN
cord-014597-66vd2mdu	1042	9	regulators	regulator	NNS
cord-014597-66vd2mdu	1042	10	including	include	VBG
cord-014597-66vd2mdu	1042	11	cyclin	cyclin	NN
cord-014597-66vd2mdu	1042	12	D1	d1	NN
cord-014597-66vd2mdu	1042	13	in	in	IN
cord-014597-66vd2mdu	1042	14	microvesicle	microvesicle	NN
cord-014597-66vd2mdu	1042	15	-	-	HYPH
cord-014597-66vd2mdu	1042	16	fraction	fraction	NN
cord-014597-66vd2mdu	1042	17	compared	compare	VBN
cord-014597-66vd2mdu	1042	18	to	to	IN
cord-014597-66vd2mdu	1042	19	microvesicle	microvesicle	NNP
cord-014597-66vd2mdu	1042	20	-	-	HYPH
cord-014597-66vd2mdu	1042	21	free	free	JJ
cord-014597-66vd2mdu	1042	22	spent	spend	VBN
cord-014597-66vd2mdu	1042	23	-	-	HYPH
cord-014597-66vd2mdu	1042	24	media	medium	NNS
cord-014597-66vd2mdu	1042	25	in	in	IN
cord-014597-66vd2mdu	1042	26	our	-PRON-	PRP$
cord-014597-66vd2mdu	1042	27	laboratory	laboratory	NN
cord-014597-66vd2mdu	1042	28	(	(	-LRB-
cord-014597-66vd2mdu	1042	29	data	datum	NNS
cord-014597-66vd2mdu	1042	30	not	not	RB
cord-014597-66vd2mdu	1042	31	shown	show	VBN
cord-014597-66vd2mdu	1042	32	)	)	-RRB-
cord-014597-66vd2mdu	1042	33	;	;	:
cord-014597-66vd2mdu	1042	34	however	however	RB
cord-014597-66vd2mdu	1042	35	,	,	,
cord-014597-66vd2mdu	1042	36	further	further	JJ
cord-014597-66vd2mdu	1042	37	investigation	investigation	NN
cord-014597-66vd2mdu	1042	38	are	be	VBP
cord-014597-66vd2mdu	1042	39	required	require	VBN
cord-014597-66vd2mdu	1042	40	to	to	TO
cord-014597-66vd2mdu	1042	41	prove	prove	VB
cord-014597-66vd2mdu	1042	42	the	the	DT
cord-014597-66vd2mdu	1042	43	hypothesis	hypothesis	NN
cord-014597-66vd2mdu	1042	44	.	.	.
cord-014597-66vd2mdu	1043	1	The	the	DT
cord-014597-66vd2mdu	1043	2	overall	overall	JJ
cord-014597-66vd2mdu	1043	3	productivity	productivity	NN
cord-014597-66vd2mdu	1043	4	of	of	IN
cord-014597-66vd2mdu	1043	5	human	human	JJ
cord-014597-66vd2mdu	1043	6	IgG	igg	NN
cord-014597-66vd2mdu	1043	7	secreting	secrete	VBG
cord-014597-66vd2mdu	1043	8	CHO	CHO	NNP
cord-014597-66vd2mdu	1043	9	cells	cell	NNS
cord-014597-66vd2mdu	1043	10	was	be	VBD
cord-014597-66vd2mdu	1043	11	also	also	RB
cord-014597-66vd2mdu	1043	12	observed	observe	VBN
cord-014597-66vd2mdu	1043	13	to	to	TO
cord-014597-66vd2mdu	1043	14	increase	increase	VB
cord-014597-66vd2mdu	1043	15	bỹ	bỹ	NN
cord-014597-66vd2mdu	1043	16	4	4	CD
cord-014597-66vd2mdu	1043	17	fold	fold	NN
cord-014597-66vd2mdu	1043	18	following	follow	VBG
cord-014597-66vd2mdu	1043	19	supplementation	supplementation	NN
cord-014597-66vd2mdu	1043	20	of	of	IN
cord-014597-66vd2mdu	1043	21	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1043	22	to	to	IN
cord-014597-66vd2mdu	1043	23	the	the	DT
cord-014597-66vd2mdu	1043	24	culture	culture	NN
cord-014597-66vd2mdu	1043	25	without	without	IN
cord-014597-66vd2mdu	1043	26	significantly	significantly	RB
cord-014597-66vd2mdu	1043	27	affecting	affect	VBG
cord-014597-66vd2mdu	1043	28	per	per	IN
cord-014597-66vd2mdu	1043	29	-	-	HYPH
cord-014597-66vd2mdu	1043	30	cell	cell	NN
cord-014597-66vd2mdu	1043	31	productivity	productivity	NN
cord-014597-66vd2mdu	1043	32	.	.	.
cord-014597-66vd2mdu	1044	1	Since	since	IN
cord-014597-66vd2mdu	1044	2	microvesicle	microvesicle	NN
cord-014597-66vd2mdu	1044	3	-	-	HYPH
cord-014597-66vd2mdu	1044	4	supplementation	supplementation	NN
cord-014597-66vd2mdu	1044	5	facilitates	facilitate	VBZ
cord-014597-66vd2mdu	1044	6	cell	cell	NN
cord-014597-66vd2mdu	1044	7	growth	growth	NN
cord-014597-66vd2mdu	1044	8	,	,	,
cord-014597-66vd2mdu	1044	9	increased	increase	VBN
cord-014597-66vd2mdu	1044	10	number	number	NN
cord-014597-66vd2mdu	1044	11	of	of	IN
cord-014597-66vd2mdu	1044	12	viable	viable	JJ
cord-014597-66vd2mdu	1044	13	producer	producer	NN
cord-014597-66vd2mdu	1044	14	cells	cell	NNS
cord-014597-66vd2mdu	1044	15	in	in	IN
cord-014597-66vd2mdu	1044	16	the	the	DT
cord-014597-66vd2mdu	1044	17	culture	culture	NN
cord-014597-66vd2mdu	1044	18	could	could	MD
cord-014597-66vd2mdu	1044	19	be	be	VB
cord-014597-66vd2mdu	1044	20	expected	expect	VBN
cord-014597-66vd2mdu	1044	21	to	to	TO
cord-014597-66vd2mdu	1044	22	be	be	VB
cord-014597-66vd2mdu	1044	23	the	the	DT
cord-014597-66vd2mdu	1044	24	basis	basis	NN
cord-014597-66vd2mdu	1044	25	of	of	IN
cord-014597-66vd2mdu	1044	26	observed	observed	JJ
cord-014597-66vd2mdu	1044	27	increase	increase	NN
cord-014597-66vd2mdu	1044	28	in	in	IN
cord-014597-66vd2mdu	1044	29	the	the	DT
cord-014597-66vd2mdu	1044	30	overall	overall	JJ
cord-014597-66vd2mdu	1044	31	productivity	productivity	NN
cord-014597-66vd2mdu	1044	32	of	of	IN
cord-014597-66vd2mdu	1044	33	the	the	DT
cord-014597-66vd2mdu	1044	34	culture	culture	NN
cord-014597-66vd2mdu	1044	35	[	[	-LRB-
cord-014597-66vd2mdu	1044	36	2	2	CD
cord-014597-66vd2mdu	1044	37	,	,	,
cord-014597-66vd2mdu	1044	38	3	3	CD
cord-014597-66vd2mdu	1044	39	]	]	-RRB-
cord-014597-66vd2mdu	1044	40	.	.	.
cord-014597-66vd2mdu	1045	1	The	the	DT
cord-014597-66vd2mdu	1045	2	further	further	JJ
cord-014597-66vd2mdu	1045	3	work	work	NN
cord-014597-66vd2mdu	1045	4	is	be	VBZ
cord-014597-66vd2mdu	1045	5	ongoing	ongoing	JJ
cord-014597-66vd2mdu	1045	6	to	to	IN
cord-014597-66vd2mdu	1045	7	in	in	IN
cord-014597-66vd2mdu	1045	8	-	-	HYPH
cord-014597-66vd2mdu	1045	9	depth	depth	NN
cord-014597-66vd2mdu	1045	10	explore	explore	VB
cord-014597-66vd2mdu	1045	11	the	the	DT
cord-014597-66vd2mdu	1045	12	potential	potential	NN
cord-014597-66vd2mdu	1045	13	of	of	IN
cord-014597-66vd2mdu	1045	14	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1045	15	for	for	IN
cord-014597-66vd2mdu	1045	16	improving	improve	VBG
cord-014597-66vd2mdu	1045	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1045	18	protein	protein	NN
cord-014597-66vd2mdu	1045	19	production	production	NN
cord-014597-66vd2mdu	1045	20	from	from	IN
cord-014597-66vd2mdu	1045	21	CHO	CHO	NNP
cord-014597-66vd2mdu	1045	22	cells	cell	NNS
cord-014597-66vd2mdu	1045	23	.	.	.
cord-014597-66vd2mdu	1046	1	The	the	DT
cord-014597-66vd2mdu	1046	2	data	datum	NNS
cord-014597-66vd2mdu	1046	3	indicate	indicate	VBP
cord-014597-66vd2mdu	1046	4	that	that	IN
cord-014597-66vd2mdu	1046	5	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1046	6	secreted	secrete	VBN
cord-014597-66vd2mdu	1046	7	from	from	IN
cord-014597-66vd2mdu	1046	8	CHO	CHO	NNP
cord-014597-66vd2mdu	1046	9	cells	cell	NNS
cord-014597-66vd2mdu	1046	10	can	can	MD
cord-014597-66vd2mdu	1046	11	improve	improve	VB
cord-014597-66vd2mdu	1046	12	cell	cell	NN
cord-014597-66vd2mdu	1046	13	growth	growth	NN
cord-014597-66vd2mdu	1046	14	and	and	CC
cord-014597-66vd2mdu	1046	15	hence	hence	RB
cord-014597-66vd2mdu	1046	16	recombinat	recombinat	NNP
cord-014597-66vd2mdu	1046	17	protein	protein	NN
cord-014597-66vd2mdu	1046	18	production	production	NN
cord-014597-66vd2mdu	1046	19	in	in	IN
cord-014597-66vd2mdu	1046	20	culture	culture	NN
cord-014597-66vd2mdu	1046	21	.	.	.
cord-014597-66vd2mdu	1047	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1047	2	,	,	,
cord-014597-66vd2mdu	1047	3	strategies	strategy	NNS
cord-014597-66vd2mdu	1047	4	need	need	VBP
cord-014597-66vd2mdu	1047	5	to	to	TO
cord-014597-66vd2mdu	1047	6	be	be	VB
cord-014597-66vd2mdu	1047	7	developed	develop	VBN
cord-014597-66vd2mdu	1047	8	for	for	IN
cord-014597-66vd2mdu	1047	9	sterile	sterile	JJ
cord-014597-66vd2mdu	1047	10	isolation	isolation	NN
cord-014597-66vd2mdu	1047	11	of	of	IN
cord-014597-66vd2mdu	1047	12	CHO	CHO	NNP
cord-014597-66vd2mdu	1047	13	microvesicles	microvesicle	NNS
cord-014597-66vd2mdu	1047	14	from	from	IN
cord-014597-66vd2mdu	1047	15	routine	routine	JJ
cord-014597-66vd2mdu	1047	16	maintenance	maintenance	NN
cord-014597-66vd2mdu	1047	17	cultures	culture	NNS
cord-014597-66vd2mdu	1047	18	and	and	CC
cord-014597-66vd2mdu	1047	19	their	-PRON-	PRP$
cord-014597-66vd2mdu	1047	20	supplementation	supplementation	NN
cord-014597-66vd2mdu	1047	21	into	into	IN
cord-014597-66vd2mdu	1047	22	the	the	DT
cord-014597-66vd2mdu	1047	23	production	production	NN
cord-014597-66vd2mdu	1047	24	culture	culture	NN
cord-014597-66vd2mdu	1047	25	for	for	IN
cord-014597-66vd2mdu	1047	26	improving	improve	VBG
cord-014597-66vd2mdu	1047	27	the	the	DT
cord-014597-66vd2mdu	1047	28	performance	performance	NN
cord-014597-66vd2mdu	1047	29	of	of	IN
cord-014597-66vd2mdu	1047	30	CHO	CHO	NNP
cord-014597-66vd2mdu	1047	31	-	-	HYPH
cord-014597-66vd2mdu	1047	32	based	base	VBN
cord-014597-66vd2mdu	1047	33	production	production	NN
cord-014597-66vd2mdu	1047	34	process	process	NN
cord-014597-66vd2mdu	1047	35	.	.	.
cord-014597-66vd2mdu	1048	1	The	the	DT
cord-014597-66vd2mdu	1048	2	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1048	3	profile	profile	NN
cord-014597-66vd2mdu	1048	4	of	of	IN
cord-014597-66vd2mdu	1048	5	a	a	DT
cord-014597-66vd2mdu	1048	6	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1048	7	protein	protein	NN
cord-014597-66vd2mdu	1048	8	is	be	VBZ
cord-014597-66vd2mdu	1048	9	one	one	CD
cord-014597-66vd2mdu	1048	10	of	of	IN
cord-014597-66vd2mdu	1048	11	the	the	DT
cord-014597-66vd2mdu	1048	12	most	most	RBS
cord-014597-66vd2mdu	1048	13	important	important	JJ
cord-014597-66vd2mdu	1048	14	attributes	attribute	NNS
cord-014597-66vd2mdu	1048	15	when	when	WRB
cord-014597-66vd2mdu	1048	16	defining	define	VBG
cord-014597-66vd2mdu	1048	17	product	product	NN
cord-014597-66vd2mdu	1048	18	quality	quality	NN
cord-014597-66vd2mdu	1048	19	.	.	.
cord-014597-66vd2mdu	1049	1	Producing	produce	VBG
cord-014597-66vd2mdu	1049	2	a	a	DT
cord-014597-66vd2mdu	1049	3	protein	protein	NN
cord-014597-66vd2mdu	1049	4	with	with	IN
cord-014597-66vd2mdu	1049	5	desired	desire	VBN
cord-014597-66vd2mdu	1049	6	characteristics	characteristic	NNS
cord-014597-66vd2mdu	1049	7	requires	require	VBZ
cord-014597-66vd2mdu	1049	8	the	the	DT
cord-014597-66vd2mdu	1049	9	ability	ability	NN
cord-014597-66vd2mdu	1049	10	to	to	TO
cord-014597-66vd2mdu	1049	11	modify	modify	VB
cord-014597-66vd2mdu	1049	12	and	and	CC
cord-014597-66vd2mdu	1049	13	target	target	VB
cord-014597-66vd2mdu	1049	14	specific	specific	JJ
cord-014597-66vd2mdu	1049	15	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1049	16	profiles	profile	NNS
cord-014597-66vd2mdu	1049	17	.	.	.
cord-014597-66vd2mdu	1050	1	Traditionally	traditionally	RB
cord-014597-66vd2mdu	1050	2	the	the	DT
cord-014597-66vd2mdu	1050	3	approach	approach	NN
cord-014597-66vd2mdu	1050	4	to	to	TO
cord-014597-66vd2mdu	1050	5	modify	modify	VB
cord-014597-66vd2mdu	1050	6	the	the	DT
cord-014597-66vd2mdu	1050	7	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1050	8	profile	profile	NN
cord-014597-66vd2mdu	1050	9	of	of	IN
cord-014597-66vd2mdu	1050	10	a	a	DT
cord-014597-66vd2mdu	1050	11	protein	protein	NN
cord-014597-66vd2mdu	1050	12	involves	involve	VBZ
cord-014597-66vd2mdu	1050	13	supplementing	supplement	VBG
cord-014597-66vd2mdu	1050	14	a	a	DT
cord-014597-66vd2mdu	1050	15	culture	culture	NN
cord-014597-66vd2mdu	1050	16	with	with	IN
cord-014597-66vd2mdu	1050	17	components	component	NNS
cord-014597-66vd2mdu	1050	18	that	that	WDT
cord-014597-66vd2mdu	1050	19	can	can	MD
cord-014597-66vd2mdu	1050	20	improve	improve	VB
cord-014597-66vd2mdu	1050	21	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1050	22	.	.	.
cord-014597-66vd2mdu	1051	1	Experimentation	experimentation	NN
cord-014597-66vd2mdu	1051	2	using	use	VBG
cord-014597-66vd2mdu	1051	3	this	this	DT
cord-014597-66vd2mdu	1051	4	supplemental	supplemental	JJ
cord-014597-66vd2mdu	1051	5	approach	approach	NN
cord-014597-66vd2mdu	1051	6	resulted	result	VBD
cord-014597-66vd2mdu	1051	7	in	in	IN
cord-014597-66vd2mdu	1051	8	a	a	DT
cord-014597-66vd2mdu	1051	9	dramatic	dramatic	JJ
cord-014597-66vd2mdu	1051	10	increase	increase	NN
cord-014597-66vd2mdu	1051	11	in	in	IN
cord-014597-66vd2mdu	1051	12	terminal	terminal	JJ
cord-014597-66vd2mdu	1051	13	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1051	14	,	,	,
cord-014597-66vd2mdu	1051	15	but	but	CC
cord-014597-66vd2mdu	1051	16	lacked	lack	VBD
cord-014597-66vd2mdu	1051	17	the	the	DT
cord-014597-66vd2mdu	1051	18	ability	ability	NN
cord-014597-66vd2mdu	1051	19	to	to	TO
cord-014597-66vd2mdu	1051	20	easily	easily	RB
cord-014597-66vd2mdu	1051	21	and	and	CC
cord-014597-66vd2mdu	1051	22	repeatedly	repeatedly	RB
cord-014597-66vd2mdu	1051	23	target	target	VB
cord-014597-66vd2mdu	1051	24	specific	specific	JJ
cord-014597-66vd2mdu	1051	25	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1051	26	profiles	profile	NNS
cord-014597-66vd2mdu	1051	27	.	.	.
cord-014597-66vd2mdu	1052	1	Using	use	VBG
cord-014597-66vd2mdu	1052	2	novel	novel	JJ
cord-014597-66vd2mdu	1052	3	and	and	CC
cord-014597-66vd2mdu	1052	4	proprietary	proprietary	JJ
cord-014597-66vd2mdu	1052	5	technology	technology	NN
cord-014597-66vd2mdu	1052	6	,	,	,
cord-014597-66vd2mdu	1052	7	we	-PRON-	PRP
cord-014597-66vd2mdu	1052	8	have	have	VBP
cord-014597-66vd2mdu	1052	9	developed	develop	VBN
cord-014597-66vd2mdu	1052	10	a	a	DT
cord-014597-66vd2mdu	1052	11	feed	feed	NN
cord-014597-66vd2mdu	1052	12	(	(	-LRB-
cord-014597-66vd2mdu	1052	13	GlycanTune	GlycanTune	NNP
cord-014597-66vd2mdu	1052	14	™	™	NN
cord-014597-66vd2mdu	1052	15	)	)	-RRB-
cord-014597-66vd2mdu	1052	16	and	and	CC
cord-014597-66vd2mdu	1052	17	a	a	DT
cord-014597-66vd2mdu	1052	18	unique	unique	JJ
cord-014597-66vd2mdu	1052	19	feeding	feeding	NN
cord-014597-66vd2mdu	1052	20	process	process	NN
cord-014597-66vd2mdu	1052	21	that	that	WDT
cord-014597-66vd2mdu	1052	22	will	will	MD
cord-014597-66vd2mdu	1052	23	maximize	maximize	VB
cord-014597-66vd2mdu	1052	24	growth	growth	NN
cord-014597-66vd2mdu	1052	25	and	and	CC
cord-014597-66vd2mdu	1052	26	titer	titer	NN
cord-014597-66vd2mdu	1052	27	while	while	IN
cord-014597-66vd2mdu	1052	28	being	be	VBG
cord-014597-66vd2mdu	1052	29	able	able	JJ
cord-014597-66vd2mdu	1052	30	to	to	TO
cord-014597-66vd2mdu	1052	31	modulate	modulate	VB
cord-014597-66vd2mdu	1052	32	glycan	glycan	NN
cord-014597-66vd2mdu	1052	33	profiles	profile	NNS
cord-014597-66vd2mdu	1052	34	.	.	.
cord-014597-66vd2mdu	1053	1	This	this	DT
cord-014597-66vd2mdu	1053	2	new	new	JJ
cord-014597-66vd2mdu	1053	3	feed	feed	NN
cord-014597-66vd2mdu	1053	4	can	can	MD
cord-014597-66vd2mdu	1053	5	be	be	VB
cord-014597-66vd2mdu	1053	6	added	add	VBN
cord-014597-66vd2mdu	1053	7	as	as	IN
cord-014597-66vd2mdu	1053	8	a	a	DT
cord-014597-66vd2mdu	1053	9	standalone	standalone	JJ
cord-014597-66vd2mdu	1053	10	process	process	NN
cord-014597-66vd2mdu	1053	11	that	that	WDT
cord-014597-66vd2mdu	1053	12	can	can	MD
cord-014597-66vd2mdu	1053	13	result	result	VB
cord-014597-66vd2mdu	1053	14	in	in	IN
cord-014597-66vd2mdu	1053	15	a	a	DT
cord-014597-66vd2mdu	1053	16	significant	significant	JJ
cord-014597-66vd2mdu	1053	17	shift	shift	NN
cord-014597-66vd2mdu	1053	18	from	from	IN
cord-014597-66vd2mdu	1053	19	G0F	g0f	NN
cord-014597-66vd2mdu	1053	20	to	to	IN
cord-014597-66vd2mdu	1053	21	G1F	g1f	NN
cord-014597-66vd2mdu	1053	22	and	and	CC
cord-014597-66vd2mdu	1053	23	G2F	g2f	LS
cord-014597-66vd2mdu	1053	24	(	(	-LRB-
cord-014597-66vd2mdu	1053	25	maximum	maximum	JJ
cord-014597-66vd2mdu	1053	26	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1053	27	)	)	-RRB-
cord-014597-66vd2mdu	1053	28	.	.	.
cord-014597-66vd2mdu	1054	1	Using	use	VBG
cord-014597-66vd2mdu	1054	2	a	a	DT
cord-014597-66vd2mdu	1054	3	unique	unique	JJ
cord-014597-66vd2mdu	1054	4	fed	fed	JJ
cord-014597-66vd2mdu	1054	5	-	-	HYPH
cord-014597-66vd2mdu	1054	6	batch	batch	NN
cord-014597-66vd2mdu	1054	7	process	process	NN
cord-014597-66vd2mdu	1054	8	,	,	,
cord-014597-66vd2mdu	1054	9	GlycanTune	GlycanTune	NNP
cord-014597-66vd2mdu	1054	10	can	can	MD
cord-014597-66vd2mdu	1054	11	also	also	RB
cord-014597-66vd2mdu	1054	12	be	be	VB
cord-014597-66vd2mdu	1054	13	used	use	VBN
cord-014597-66vd2mdu	1054	14	with	with	IN
cord-014597-66vd2mdu	1054	15	a	a	DT
cord-014597-66vd2mdu	1054	16	standard	standard	JJ
cord-014597-66vd2mdu	1054	17	feed	feed	NN
cord-014597-66vd2mdu	1054	18	to	to	TO
cord-014597-66vd2mdu	1054	19	dial	dial	VB
cord-014597-66vd2mdu	1054	20	in	in	IN
cord-014597-66vd2mdu	1054	21	targeted	target	VBN
cord-014597-66vd2mdu	1054	22	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1054	23	profiles	profile	NNS
cord-014597-66vd2mdu	1054	24	.	.	.
cord-014597-66vd2mdu	1055	1	Through	through	IN
cord-014597-66vd2mdu	1055	2	process	process	NN
cord-014597-66vd2mdu	1055	3	development	development	NN
cord-014597-66vd2mdu	1055	4	,	,	,
cord-014597-66vd2mdu	1055	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1055	6	created	create	VBD
cord-014597-66vd2mdu	1055	7	a	a	DT
cord-014597-66vd2mdu	1055	8	method	method	NN
cord-014597-66vd2mdu	1055	9	where	where	WRB
cord-014597-66vd2mdu	1055	10	a	a	DT
cord-014597-66vd2mdu	1055	11	transition	transition	NN
cord-014597-66vd2mdu	1055	12	point	point	NN
cord-014597-66vd2mdu	1055	13	is	be	VBZ
cord-014597-66vd2mdu	1055	14	used	use	VBN
cord-014597-66vd2mdu	1055	15	to	to	TO
cord-014597-66vd2mdu	1055	16	switch	switch	VB
cord-014597-66vd2mdu	1055	17	from	from	IN
cord-014597-66vd2mdu	1055	18	a	a	DT
cord-014597-66vd2mdu	1055	19	standard	standard	JJ
cord-014597-66vd2mdu	1055	20	feed	feed	NN
cord-014597-66vd2mdu	1055	21	to	to	IN
cord-014597-66vd2mdu	1055	22	a	a	DT
cord-014597-66vd2mdu	1055	23	glycan	glycan	NN
cord-014597-66vd2mdu	1055	24	modulating	modulate	VBG
cord-014597-66vd2mdu	1055	25	feed	feed	NN
cord-014597-66vd2mdu	1055	26	.	.	.
cord-014597-66vd2mdu	1056	1	The	the	DT
cord-014597-66vd2mdu	1056	2	timing	timing	NN
cord-014597-66vd2mdu	1056	3	of	of	IN
cord-014597-66vd2mdu	1056	4	the	the	DT
cord-014597-66vd2mdu	1056	5	transition	transition	NN
cord-014597-66vd2mdu	1056	6	point	point	NN
cord-014597-66vd2mdu	1056	7	will	will	MD
cord-014597-66vd2mdu	1056	8	determine	determine	VB
cord-014597-66vd2mdu	1056	9	the	the	DT
cord-014597-66vd2mdu	1056	10	specificity	specificity	NN
cord-014597-66vd2mdu	1056	11	of	of	IN
cord-014597-66vd2mdu	1056	12	the	the	DT
cord-014597-66vd2mdu	1056	13	glycan	glycan	NN
cord-014597-66vd2mdu	1056	14	profile	profile	NN
cord-014597-66vd2mdu	1056	15	.	.	.
cord-014597-66vd2mdu	1056	16	)	)	-RRB-
cord-014597-66vd2mdu	1056	17	.	.	.
cord-014597-66vd2mdu	1057	1	N	n	CD
cord-014597-66vd2mdu	1057	2	-	-	HYPH
cord-014597-66vd2mdu	1057	3	linked	link	VBN
cord-014597-66vd2mdu	1057	4	glycans	glycan	NNS
cord-014597-66vd2mdu	1057	5	were	be	VBD
cord-014597-66vd2mdu	1057	6	digested	digest	VBN
cord-014597-66vd2mdu	1057	7	with	with	IN
cord-014597-66vd2mdu	1057	8	PNGase	PNGase	NNP
cord-014597-66vd2mdu	1057	9	F	F	NNP
cord-014597-66vd2mdu	1057	10	and	and	CC
cord-014597-66vd2mdu	1057	11	quantified	quantify	VBD
cord-014597-66vd2mdu	1057	12	using	use	VBG
cord-014597-66vd2mdu	1057	13	100pmole	100pmole	NNP
cord-014597-66vd2mdu	1057	14	maltohexose	maltohexose	NNP
cord-014597-66vd2mdu	1057	15	/	/	SYM
cord-014597-66vd2mdu	1057	16	maltopentose	maltopentose	NNP
cord-014597-66vd2mdu	1057	17	internal	internal	JJ
cord-014597-66vd2mdu	1057	18	standards	standard	NNS
cord-014597-66vd2mdu	1057	19	labeled	label	VBN
cord-014597-66vd2mdu	1057	20	with	with	IN
cord-014597-66vd2mdu	1057	21	8-aminopyrene-1,3,6-trisulfonic	8-aminopyrene-1,3,6-trisulfonic	CD
cord-014597-66vd2mdu	1057	22	acid	acid	NN
cord-014597-66vd2mdu	1057	23	(	(	-LRB-
cord-014597-66vd2mdu	1057	24	ATPS	ATPS	NNP
cord-014597-66vd2mdu	1057	25	)	)	-RRB-
cord-014597-66vd2mdu	1057	26	as	as	IN
cord-014597-66vd2mdu	1057	27	described	describe	VBN
cord-014597-66vd2mdu	1057	28	by	by	IN
cord-014597-66vd2mdu	1057	29	Laroy	Laroy	NNP
cord-014597-66vd2mdu	1057	30	et	et	FW
cord-014597-66vd2mdu	1057	31	al	al	NNP
cord-014597-66vd2mdu	1057	32	[	[	-LRB-
cord-014597-66vd2mdu	1057	33	1	1	CD
cord-014597-66vd2mdu	1057	34	]	]	-RRB-
cord-014597-66vd2mdu	1057	35	or	or	CC
cord-014597-66vd2mdu	1057	36	the	the	DT
cord-014597-66vd2mdu	1057	37	user	user	NN
cord-014597-66vd2mdu	1057	38	guide	guide	NN
cord-014597-66vd2mdu	1057	39	for	for	IN
cord-014597-66vd2mdu	1057	40	the	the	DT
cord-014597-66vd2mdu	1057	41	Glycan	Glycan	NNP
cord-014597-66vd2mdu	1057	42	Labeling	Labeling	NNP
cord-014597-66vd2mdu	1057	43	and	and	CC
cord-014597-66vd2mdu	1057	44	Analysis	Analysis	NNP
cord-014597-66vd2mdu	1057	45	Kit	kit	NN
cord-014597-66vd2mdu	1057	46	(	(	-LRB-
cord-014597-66vd2mdu	1057	47	GlycanAssure	GlycanAssure	NNP
cord-014597-66vd2mdu	1057	48	™	™	NN
cord-014597-66vd2mdu	1057	49	user	user	NN
cord-014597-66vd2mdu	1057	50	guide	guide	NN
cord-014597-66vd2mdu	1057	51	,	,	,
cord-014597-66vd2mdu	1057	52	Thermo	Thermo	NNP
cord-014597-66vd2mdu	1057	53	Fisher	Fisher	NNP
cord-014597-66vd2mdu	1057	54	Scientific	Scientific	NNP
cord-014597-66vd2mdu	1057	55	)	)	-RRB-
cord-014597-66vd2mdu	1057	56	.	.	.
cord-014597-66vd2mdu	1058	1	All	all	DT
cord-014597-66vd2mdu	1058	2	CE	CE	NNP
cord-014597-66vd2mdu	1058	3	separations	separation	NNS
cord-014597-66vd2mdu	1058	4	were	be	VBD
cord-014597-66vd2mdu	1058	5	performed	perform	VBN
cord-014597-66vd2mdu	1058	6	using	use	VBG
cord-014597-66vd2mdu	1058	7	the	the	DT
cord-014597-66vd2mdu	1058	8	Applied	Applied	NNP
cord-014597-66vd2mdu	1058	9	Biosystems	Biosystems	NNP
cord-014597-66vd2mdu	1058	10	™	™	CD
cord-014597-66vd2mdu	1058	11	3500xL.	3500xl.	CD
cord-014597-66vd2mdu	1059	1	The	the	DT
cord-014597-66vd2mdu	1059	2	timing	timing	NN
cord-014597-66vd2mdu	1059	3	of	of	IN
cord-014597-66vd2mdu	1059	4	transition	transition	NN
cord-014597-66vd2mdu	1059	5	from	from	IN
cord-014597-66vd2mdu	1059	6	EFC+	EFC+	NNP
cord-014597-66vd2mdu	1059	7	to	to	IN
cord-014597-66vd2mdu	1059	8	GTC+	GTC+	NNP
cord-014597-66vd2mdu	1059	9	made	make	VBD
cord-014597-66vd2mdu	1059	10	it	-PRON-	PRP
cord-014597-66vd2mdu	1059	11	possible	possible	JJ
cord-014597-66vd2mdu	1059	12	to	to	TO
cord-014597-66vd2mdu	1059	13	target	target	VB
cord-014597-66vd2mdu	1059	14	specific	specific	JJ
cord-014597-66vd2mdu	1059	15	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1059	16	profiles	profile	NNS
cord-014597-66vd2mdu	1059	17	.	.	.
cord-014597-66vd2mdu	1060	1	Modulating	modulate	VBG
cord-014597-66vd2mdu	1060	2	G0F	G0F	NNS
cord-014597-66vd2mdu	1060	3	from	from	IN
cord-014597-66vd2mdu	1060	4	75	75	CD
cord-014597-66vd2mdu	1060	5	%	%	NN
cord-014597-66vd2mdu	1060	6	down	down	RB
cord-014597-66vd2mdu	1060	7	to	to	IN
cord-014597-66vd2mdu	1060	8	32	32	CD
cord-014597-66vd2mdu	1060	9	%	%	NN
cord-014597-66vd2mdu	1060	10	,	,	,
cord-014597-66vd2mdu	1060	11	while	while	IN
cord-014597-66vd2mdu	1060	12	increasing	increase	VBG
cord-014597-66vd2mdu	1060	13	G1F	g1f	RB
cord-014597-66vd2mdu	1060	14	and	and	CC
cord-014597-66vd2mdu	1060	15	increasing	increase	VBG
cord-014597-66vd2mdu	1060	16	G2F	G2F	NNS
cord-014597-66vd2mdu	1060	17	(	(	-LRB-
cord-014597-66vd2mdu	1060	18	Fig	Fig	NNP
cord-014597-66vd2mdu	1060	19	.	.	.
cord-014597-66vd2mdu	1060	20	1	1	CD
cord-014597-66vd2mdu	1060	21	)	)	-RRB-
cord-014597-66vd2mdu	1060	22	.	.	.
cord-014597-66vd2mdu	1061	1	Transitioning	transition	VBG
cord-014597-66vd2mdu	1061	2	to	to	IN
cord-014597-66vd2mdu	1061	3	GTC+	GTC+	NNS
cord-014597-66vd2mdu	1061	4	early	early	RB
cord-014597-66vd2mdu	1061	5	in	in	IN
cord-014597-66vd2mdu	1061	6	culture	culture	NN
cord-014597-66vd2mdu	1061	7	resulted	result	VBD
cord-014597-66vd2mdu	1061	8	in	in	IN
cord-014597-66vd2mdu	1061	9	a	a	DT
cord-014597-66vd2mdu	1061	10	greater	great	JJR
cord-014597-66vd2mdu	1061	11	shift	shift	NN
cord-014597-66vd2mdu	1061	12	from	from	IN
cord-014597-66vd2mdu	1061	13	G0F	g0f	NN
cord-014597-66vd2mdu	1061	14	to	to	IN
cord-014597-66vd2mdu	1061	15	G1F	g1f	CD
cord-014597-66vd2mdu	1061	16	and	and	CC
cord-014597-66vd2mdu	1061	17	G2F.	G2F.	NNP
cord-014597-66vd2mdu	1061	18	Transitioning	transition	VBG
cord-014597-66vd2mdu	1061	19	midway	midway	RB
cord-014597-66vd2mdu	1061	20	or	or	CC
cord-014597-66vd2mdu	1061	21	late	late	RB
cord-014597-66vd2mdu	1061	22	in	in	IN
cord-014597-66vd2mdu	1061	23	culture	culture	NN
cord-014597-66vd2mdu	1061	24	resulted	result	VBD
cord-014597-66vd2mdu	1061	25	in	in	IN
cord-014597-66vd2mdu	1061	26	a	a	DT
cord-014597-66vd2mdu	1061	27	greater	great	JJR
cord-014597-66vd2mdu	1061	28	proportion	proportion	NN
cord-014597-66vd2mdu	1061	29	of	of	IN
cord-014597-66vd2mdu	1061	30	G0F	g0f	NN
cord-014597-66vd2mdu	1061	31	compared	compare	VBN
cord-014597-66vd2mdu	1061	32	to	to	IN
cord-014597-66vd2mdu	1061	33	G1F	g1f	NN
cord-014597-66vd2mdu	1061	34	and	and	CC
cord-014597-66vd2mdu	1061	35	G2F.	G2F.	NNP
cord-014597-66vd2mdu	1061	36	Supplementation	Supplementation	NNP
cord-014597-66vd2mdu	1061	37	based	base	VBN
cord-014597-66vd2mdu	1061	38	approaches	approach	NNS
cord-014597-66vd2mdu	1061	39	using	use	VBG
cord-014597-66vd2mdu	1061	40	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1061	41	modulating	modulate	VBG
cord-014597-66vd2mdu	1061	42	media	medium	NNS
cord-014597-66vd2mdu	1061	43	components	component	NNS
cord-014597-66vd2mdu	1061	44	to	to	TO
cord-014597-66vd2mdu	1061	45	modify	modify	VB
cord-014597-66vd2mdu	1061	46	and	and	CC
cord-014597-66vd2mdu	1061	47	target	target	VB
cord-014597-66vd2mdu	1061	48	specific	specific	JJ
cord-014597-66vd2mdu	1061	49	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1061	50	profiles	profile	NNS
cord-014597-66vd2mdu	1061	51	proved	prove	VBD
cord-014597-66vd2mdu	1061	52	to	to	TO
cord-014597-66vd2mdu	1061	53	be	be	VB
cord-014597-66vd2mdu	1061	54	difficult	difficult	JJ
cord-014597-66vd2mdu	1061	55	.	.	.
cord-014597-66vd2mdu	1062	1	These	these	DT
cord-014597-66vd2mdu	1062	2	approaches	approach	NNS
cord-014597-66vd2mdu	1062	3	were	be	VBD
cord-014597-66vd2mdu	1062	4	able	able	JJ
cord-014597-66vd2mdu	1062	5	to	to	TO
cord-014597-66vd2mdu	1062	6	increase	increase	VB
cord-014597-66vd2mdu	1062	7	terminal	terminal	JJ
cord-014597-66vd2mdu	1062	8	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1062	9	(	(	-LRB-
cord-014597-66vd2mdu	1062	10	G1F	g1f	NN
cord-014597-66vd2mdu	1062	11	and	and	CC
cord-014597-66vd2mdu	1062	12	G2F	G2F	NNP
cord-014597-66vd2mdu	1062	13	)	)	-RRB-
cord-014597-66vd2mdu	1062	14	,	,	,
cord-014597-66vd2mdu	1062	15	but	but	CC
cord-014597-66vd2mdu	1062	16	lacked	lack	VBD
cord-014597-66vd2mdu	1062	17	the	the	DT
cord-014597-66vd2mdu	1062	18	ability	ability	NN
cord-014597-66vd2mdu	1062	19	to	to	TO
cord-014597-66vd2mdu	1062	20	fine	fine	VB
cord-014597-66vd2mdu	1062	21	tune	tune	NN
cord-014597-66vd2mdu	1062	22	glycan	glycan	NN
cord-014597-66vd2mdu	1062	23	profiles	profile	NNS
cord-014597-66vd2mdu	1062	24	.	.	.
cord-014597-66vd2mdu	1063	1	This	this	DT
cord-014597-66vd2mdu	1063	2	could	could	MD
cord-014597-66vd2mdu	1063	3	result	result	VB
cord-014597-66vd2mdu	1063	4	in	in	IN
cord-014597-66vd2mdu	1063	5	numerous	numerous	JJ
cord-014597-66vd2mdu	1063	6	rounds	round	NNS
cord-014597-66vd2mdu	1063	7	of	of	IN
cord-014597-66vd2mdu	1063	8	titration	titration	NN
cord-014597-66vd2mdu	1063	9	experiments	experiment	NNS
cord-014597-66vd2mdu	1063	10	to	to	TO
cord-014597-66vd2mdu	1063	11	target	target	VB
cord-014597-66vd2mdu	1063	12	specific	specific	JJ
cord-014597-66vd2mdu	1063	13	glycan	glycan	NN
cord-014597-66vd2mdu	1063	14	profiles	profile	NNS
cord-014597-66vd2mdu	1063	15	that	that	WDT
cord-014597-66vd2mdu	1063	16	would	would	MD
cord-014597-66vd2mdu	1063	17	likely	likely	RB
cord-014597-66vd2mdu	1063	18	remain	remain	VB
cord-014597-66vd2mdu	1063	19	inconsistent	inconsistent	JJ
cord-014597-66vd2mdu	1063	20	between	between	IN
cord-014597-66vd2mdu	1063	21	cell	cell	NN
cord-014597-66vd2mdu	1063	22	lines	line	NNS
cord-014597-66vd2mdu	1063	23	,	,	,
cord-014597-66vd2mdu	1063	24	culture	culture	NN
cord-014597-66vd2mdu	1063	25	media	medium	NNS
cord-014597-66vd2mdu	1063	26	and	and	CC
cord-014597-66vd2mdu	1063	27	feeds	feed	NNS
cord-014597-66vd2mdu	1063	28	,	,	,
cord-014597-66vd2mdu	1063	29	and	and	CC
cord-014597-66vd2mdu	1063	30	process	process	NN
cord-014597-66vd2mdu	1063	31	scale	scale	NN
cord-014597-66vd2mdu	1063	32	.	.	.
cord-014597-66vd2mdu	1064	1	The	the	DT
cord-014597-66vd2mdu	1064	2	development	development	NN
cord-014597-66vd2mdu	1064	3	of	of	IN
cord-014597-66vd2mdu	1064	4	a	a	DT
cord-014597-66vd2mdu	1064	5	unique	unique	JJ
cord-014597-66vd2mdu	1064	6	process	process	NN
cord-014597-66vd2mdu	1064	7	made	make	VBD
cord-014597-66vd2mdu	1064	8	it	-PRON-	PRP
cord-014597-66vd2mdu	1064	9	possible	possible	JJ
cord-014597-66vd2mdu	1064	10	to	to	TO
cord-014597-66vd2mdu	1064	11	predictably	predictably	RB
cord-014597-66vd2mdu	1064	12	target	target	VB
cord-014597-66vd2mdu	1064	13	specific	specific	JJ
cord-014597-66vd2mdu	1064	14	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1064	15	profiles	profile	NNS
cord-014597-66vd2mdu	1064	16	.	.	.
cord-014597-66vd2mdu	1065	1	Transition	transition	NN
cord-014597-66vd2mdu	1065	2	from	from	IN
cord-014597-66vd2mdu	1065	3	standard	standard	JJ
cord-014597-66vd2mdu	1065	4	feeding	feeding	NN
cord-014597-66vd2mdu	1065	5	to	to	IN
cord-014597-66vd2mdu	1065	6	GlycanTune	GlycanTune	NNP
cord-014597-66vd2mdu	1065	7	allowed	allow	VBN
cord-014597-66vd2mdu	1065	8	for	for	IN
cord-014597-66vd2mdu	1065	9	precise	precise	JJ
cord-014597-66vd2mdu	1065	10	targeting	targeting	NN
cord-014597-66vd2mdu	1065	11	of	of	IN
cord-014597-66vd2mdu	1065	12	glycan	glycan	NN
cord-014597-66vd2mdu	1065	13	profiles	profile	NNS
cord-014597-66vd2mdu	1065	14	.	.	.
cord-014597-66vd2mdu	1066	1	Transition	transition	NN
cord-014597-66vd2mdu	1066	2	to	to	IN
cord-014597-66vd2mdu	1066	3	GlycanTune	GlycanTune	NNP
cord-014597-66vd2mdu	1066	4	early	early	RB
cord-014597-66vd2mdu	1066	5	in	in	IN
cord-014597-66vd2mdu	1066	6	culture	culture	NN
cord-014597-66vd2mdu	1066	7	resulted	result	VBD
cord-014597-66vd2mdu	1066	8	in	in	IN
cord-014597-66vd2mdu	1066	9	an	an	DT
cord-014597-66vd2mdu	1066	10	increased	increased	JJ
cord-014597-66vd2mdu	1066	11	shift	shift	NN
cord-014597-66vd2mdu	1066	12	from	from	IN
cord-014597-66vd2mdu	1066	13	G0F	g0f	NN
cord-014597-66vd2mdu	1066	14	to	to	IN
cord-014597-66vd2mdu	1066	15	G1F	g1f	CD
cord-014597-66vd2mdu	1066	16	and	and	CC
cord-014597-66vd2mdu	1066	17	G2F.	G2F.	NNP
cord-014597-66vd2mdu	1066	18	A	a	DT
cord-014597-66vd2mdu	1066	19	transition	transition	NN
cord-014597-66vd2mdu	1066	20	late	late	RB
cord-014597-66vd2mdu	1066	21	in	in	IN
cord-014597-66vd2mdu	1066	22	culture	culture	NN
cord-014597-66vd2mdu	1066	23	resulted	result	VBD
cord-014597-66vd2mdu	1066	24	in	in	IN
cord-014597-66vd2mdu	1066	25	increased	increase	VBN
cord-014597-66vd2mdu	1066	26	G0F	g0f	NN
cord-014597-66vd2mdu	1066	27	and	and	CC
cord-014597-66vd2mdu	1066	28	decreased	decrease	VBD
cord-014597-66vd2mdu	1066	29	G1F	g1f	RB
cord-014597-66vd2mdu	1066	30	and	and	CC
cord-014597-66vd2mdu	1066	31	G2F.	G2F.	NNP
cord-014597-66vd2mdu	1066	32	 	 	_SP
cord-014597-66vd2mdu	1066	33	Growth	Growth	NNP
cord-014597-66vd2mdu	1066	34	performance	performance	NN
cord-014597-66vd2mdu	1066	35	during	during	IN
cord-014597-66vd2mdu	1066	36	precultures	preculture	NNS
cord-014597-66vd2mdu	1066	37	and	and	CC
cord-014597-66vd2mdu	1066	38	batch	batch	NN
cord-014597-66vd2mdu	1066	39	curves	curve	NNS
cord-014597-66vd2mdu	1066	40	in	in	IN
cord-014597-66vd2mdu	1066	41	plain	plain	JJ
cord-014597-66vd2mdu	1066	42	shaking	shaking	NN
cord-014597-66vd2mdu	1066	43	flasks	flask	NNS
cord-014597-66vd2mdu	1066	44	did	do	VBD
cord-014597-66vd2mdu	1066	45	not	not	RB
cord-014597-66vd2mdu	1066	46	show	show	VB
cord-014597-66vd2mdu	1066	47	any	any	DT
cord-014597-66vd2mdu	1066	48	differences	difference	NNS
cord-014597-66vd2mdu	1066	49	among	among	IN
cord-014597-66vd2mdu	1066	50	tested	test	VBN
cord-014597-66vd2mdu	1066	51	surfactants	surfactant	NNS
cord-014597-66vd2mdu	1066	52	or	or	CC
cord-014597-66vd2mdu	1066	53	lots	lot	NNS
cord-014597-66vd2mdu	1066	54	thereof	thereof	RB
cord-014597-66vd2mdu	1066	55	,	,	,
cord-014597-66vd2mdu	1066	56	and	and	CC
cord-014597-66vd2mdu	1066	57	cell	cell	NN
cord-014597-66vd2mdu	1066	58	densities	density	NNS
cord-014597-66vd2mdu	1066	59	reached	reach	VBD
cord-014597-66vd2mdu	1066	60	10	10	CD
cord-014597-66vd2mdu	1066	61	-	-	SYM
cord-014597-66vd2mdu	1066	62	12·10	12·10	CD
cord-014597-66vd2mdu	1066	63	6	6	CD
cord-014597-66vd2mdu	1066	64	cells/	cells/	CD
cord-014597-66vd2mdu	1066	65	mL	ml	NN
cord-014597-66vd2mdu	1067	1	(	(	-LRB-
cord-014597-66vd2mdu	1067	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1067	3	.	.	.
cord-014597-66vd2mdu	1067	4	1a	1a	CD
cord-014597-66vd2mdu	1067	5	and	and	CC
cord-014597-66vd2mdu	1067	6	b	b	LS
cord-014597-66vd2mdu	1067	7	)	)	-RRB-
cord-014597-66vd2mdu	1067	8	.	.	.
cord-014597-66vd2mdu	1068	1	Experiments	experiment	NNS
cord-014597-66vd2mdu	1068	2	with	with	IN
cord-014597-66vd2mdu	1068	3	HEK	HEK	NNP
cord-014597-66vd2mdu	1068	4	293-F	293-f	CD
cord-014597-66vd2mdu	1068	5	cells	cell	NNS
cord-014597-66vd2mdu	1068	6	at	at	IN
cord-014597-66vd2mdu	1068	7	elevated	elevated	JJ
cord-014597-66vd2mdu	1068	8	power	power	NN
cord-014597-66vd2mdu	1068	9	input	input	NN
cord-014597-66vd2mdu	1068	10	in	in	IN
cord-014597-66vd2mdu	1068	11	baffled	baffled	JJ
cord-014597-66vd2mdu	1068	12	shaking	shake	VBG
cord-014597-66vd2mdu	1068	13	flasks	flask	NNS
cord-014597-66vd2mdu	1068	14	revealed	reveal	VBD
cord-014597-66vd2mdu	1068	15	distinct	distinct	JJ
cord-014597-66vd2mdu	1068	16	differences	difference	NNS
cord-014597-66vd2mdu	1068	17	between	between	IN
cord-014597-66vd2mdu	1068	18	Pluronic	Pluronic	NNP
cord-014597-66vd2mdu	1068	19	®	®	.
cord-014597-66vd2mdu	1068	20	F-68	F-68	NNP
cord-014597-66vd2mdu	1068	21	,	,	,
cord-014597-66vd2mdu	1068	22	F-127	f-127	NN
cord-014597-66vd2mdu	1068	23	and	and	CC
cord-014597-66vd2mdu	1068	24	Kolliphor	Kolliphor	NNP
cord-014597-66vd2mdu	1068	25	®	®	,
cord-014597-66vd2mdu	1068	26	P188	P188	NNP
cord-014597-66vd2mdu	1068	27	,	,	,
cord-014597-66vd2mdu	1068	28	with	with	IN
cord-014597-66vd2mdu	1068	29	F-127	f-127	NN
cord-014597-66vd2mdu	1068	30	showing	show	VBG
cord-014597-66vd2mdu	1068	31	the	the	DT
cord-014597-66vd2mdu	1068	32	best	good	JJS
cord-014597-66vd2mdu	1068	33	performance	performance	NN
cord-014597-66vd2mdu	1068	34	.	.	.
cord-014597-66vd2mdu	1069	1	Peak	peak	JJ
cord-014597-66vd2mdu	1069	2	viable	viable	JJ
cord-014597-66vd2mdu	1069	3	cell	cell	NN
cord-014597-66vd2mdu	1069	4	densities	density	NNS
cord-014597-66vd2mdu	1069	5	reached	reach	VBN
cord-014597-66vd2mdu	1069	6	with	with	IN
cord-014597-66vd2mdu	1069	7	lots	lot	NNS
cord-014597-66vd2mdu	1069	8	A	a	NN
cord-014597-66vd2mdu	1069	9	and	and	CC
cord-014597-66vd2mdu	1069	10	B	b	NN
cord-014597-66vd2mdu	1069	11	of	of	IN
cord-014597-66vd2mdu	1069	12	Pluronic	Pluronic	NNP
cord-014597-66vd2mdu	1069	13	®	®	.
cord-014597-66vd2mdu	1069	14	F-68	F-68	NNP
cord-014597-66vd2mdu	1069	15	and	and	CC
cord-014597-66vd2mdu	1069	16	F-127	f-127	NN
cord-014597-66vd2mdu	1069	17	were	be	VBD
cord-014597-66vd2mdu	1069	18	comparable	comparable	JJ
cord-014597-66vd2mdu	1069	19	to	to	IN
cord-014597-66vd2mdu	1069	20	those	those	DT
cord-014597-66vd2mdu	1069	21	in	in	IN
cord-014597-66vd2mdu	1069	22	plain	plain	JJ
cord-014597-66vd2mdu	1069	23	shaking	shaking	NN
cord-014597-66vd2mdu	1069	24	flasks	flask	NNS
cord-014597-66vd2mdu	1069	25	,	,	,
cord-014597-66vd2mdu	1069	26	while	while	IN
cord-014597-66vd2mdu	1069	27	those	those	DT
cord-014597-66vd2mdu	1069	28	for	for	IN
cord-014597-66vd2mdu	1069	29	Kolliphor	Kolliphor	NNP
cord-014597-66vd2mdu	1069	30	®	®	POS
cord-014597-66vd2mdu	1069	31	P188	P188	NNP
cord-014597-66vd2mdu	1069	32	and	and	CC
cord-014597-66vd2mdu	1069	33	lots	lot	NNS
cord-014597-66vd2mdu	1069	34	C	c	NN
cord-014597-66vd2mdu	1069	35	and	and	CC
cord-014597-66vd2mdu	1069	36	D	d	NN
cord-014597-66vd2mdu	1069	37	of	of	IN
cord-014597-66vd2mdu	1069	38	Pluronic	Pluronic	NNP
cord-014597-66vd2mdu	1070	1	®	®	,
cord-014597-66vd2mdu	1070	2	F-68	F-68	NNP
cord-014597-66vd2mdu	1070	3	were	be	VBD
cord-014597-66vd2mdu	1070	4	significantly	significantly	RB
cord-014597-66vd2mdu	1070	5	lower	low	JJR
cord-014597-66vd2mdu	1070	6	.	.	.
cord-014597-66vd2mdu	1071	1	Peak	Peak	NNP
cord-014597-66vd2mdu	1071	2	viale	viale	NN
cord-014597-66vd2mdu	1071	3	cell	cell	NN
cord-014597-66vd2mdu	1071	4	densities	density	NNS
cord-014597-66vd2mdu	1071	5	were	be	VBD
cord-014597-66vd2mdu	1071	6	of	of	IN
cord-014597-66vd2mdu	1071	7	2	2	CD
cord-014597-66vd2mdu	1071	8	-12·10	-12·10	SYM
cord-014597-66vd2mdu	1071	9	6	6	CD
cord-014597-66vd2mdu	1071	10	cells	cell	NNS
cord-014597-66vd2mdu	1071	11	/	/	SYM
cord-014597-66vd2mdu	1071	12	mL	ml	NN
cord-014597-66vd2mdu	1072	1	(	(	-LRB-
cord-014597-66vd2mdu	1072	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1072	3	.	.	.
cord-014597-66vd2mdu	1072	4	1c	1c	CD
cord-014597-66vd2mdu	1072	5	)	)	-RRB-
cord-014597-66vd2mdu	1072	6	.	.	.
cord-014597-66vd2mdu	1073	1	Similar	similar	JJ
cord-014597-66vd2mdu	1073	2	transient	transient	JJ
cord-014597-66vd2mdu	1073	3	transfection	transfection	NN
cord-014597-66vd2mdu	1073	4	efficiency	efficiency	NN
cord-014597-66vd2mdu	1073	5	and	and	CC
cord-014597-66vd2mdu	1073	6	mean	mean	VB
cord-014597-66vd2mdu	1073	7	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	1073	8	of	of	IN
cord-014597-66vd2mdu	1073	9	transfected	transfecte	VBN
cord-014597-66vd2mdu	1073	10	cells	cell	NNS
cord-014597-66vd2mdu	1073	11	independent	independent	JJ
cord-014597-66vd2mdu	1073	12	of	of	IN
cord-014597-66vd2mdu	1073	13	applied	applied	JJ
cord-014597-66vd2mdu	1073	14	surfactant	surfactant	NN
cord-014597-66vd2mdu	1073	15	and	and	CC
cord-014597-66vd2mdu	1073	16	lot	lot	NN
cord-014597-66vd2mdu	1073	17	thereof	thereof	RB
cord-014597-66vd2mdu	1073	18	indicated	indicate	VBD
cord-014597-66vd2mdu	1073	19	no	no	DT
cord-014597-66vd2mdu	1073	20	major	major	JJ
cord-014597-66vd2mdu	1073	21	impact	impact	NN
cord-014597-66vd2mdu	1073	22	of	of	IN
cord-014597-66vd2mdu	1073	23	respective	respective	JJ
cord-014597-66vd2mdu	1073	24	poloxamer	poloxamer	NN
cord-014597-66vd2mdu	1073	25	(	(	-LRB-
cord-014597-66vd2mdu	1073	26	Fig	Fig	NNP
cord-014597-66vd2mdu	1073	27	.	.	.
cord-014597-66vd2mdu	1073	28	1d	1d	CD
cord-014597-66vd2mdu	1073	29	)	)	-RRB-
cord-014597-66vd2mdu	1073	30	.	.	.
cord-014597-66vd2mdu	1074	1	Interestingly	interestingly	RB
cord-014597-66vd2mdu	1074	2	,	,	,
cord-014597-66vd2mdu	1074	3	experiments	experiment	NNS
cord-014597-66vd2mdu	1074	4	using	use	VBG
cord-014597-66vd2mdu	1074	5	fluorescein	fluorescein	NN
cord-014597-66vd2mdu	1074	6	-	-	HYPH
cord-014597-66vd2mdu	1074	7	labelled	label	VBN
cord-014597-66vd2mdu	1074	8	Pluronic	Pluronic	NNP
cord-014597-66vd2mdu	1074	9	®	®	NNS
cord-014597-66vd2mdu	1074	10	showed	show	VBD
cord-014597-66vd2mdu	1074	11	a	a	DT
cord-014597-66vd2mdu	1074	12	time	time	NN
cord-014597-66vd2mdu	1074	13	-	-	HYPH
cord-014597-66vd2mdu	1074	14	dependent	dependent	JJ
cord-014597-66vd2mdu	1074	15	uptake	uptake	NN
cord-014597-66vd2mdu	1074	16	into	into	IN
cord-014597-66vd2mdu	1074	17	HEK	HEK	NNP
cord-014597-66vd2mdu	1074	18	cells	cell	NNS
cord-014597-66vd2mdu	1074	19	.	.	.
cord-014597-66vd2mdu	1075	1	Visual	visual	JJ
cord-014597-66vd2mdu	1075	2	tracking	tracking	NN
cord-014597-66vd2mdu	1075	3	revealed	reveal	VBD
cord-014597-66vd2mdu	1075	4	an	an	DT
cord-014597-66vd2mdu	1075	5	endocytic	endocytic	JJ
cord-014597-66vd2mdu	1075	6	uptake	uptake	NN
cord-014597-66vd2mdu	1075	7	of	of	IN
cord-014597-66vd2mdu	1075	8	poloxamers	poloxamer	NNS
cord-014597-66vd2mdu	1075	9	by	by	IN
cord-014597-66vd2mdu	1075	10	the	the	DT
cord-014597-66vd2mdu	1075	11	cells	cell	NNS
cord-014597-66vd2mdu	1075	12	(	(	-LRB-
cord-014597-66vd2mdu	1075	13	>	>	XX
cord-014597-66vd2mdu	1076	1	10fold	10fold	CD
cord-014597-66vd2mdu	1076	2	increase	increase	NN
cord-014597-66vd2mdu	1076	3	in	in	IN
cord-014597-66vd2mdu	1076	4	signal	signal	NN
cord-014597-66vd2mdu	1076	5	after	after	IN
cord-014597-66vd2mdu	1076	6	96	96	CD
cord-014597-66vd2mdu	1076	7	h	h	NN
cord-014597-66vd2mdu	1076	8	)	)	-RRB-
cord-014597-66vd2mdu	1076	9	and	and	CC
cord-014597-66vd2mdu	1076	10	its	-PRON-	PRP$
cord-014597-66vd2mdu	1076	11	co	co	NN
cord-014597-66vd2mdu	1076	12	-	-	NN
cord-014597-66vd2mdu	1076	13	localisation	localisation	NN
cord-014597-66vd2mdu	1076	14	with	with	IN
cord-014597-66vd2mdu	1076	15	cell	cell	NN
cord-014597-66vd2mdu	1076	16	membrane	membrane	NN
cord-014597-66vd2mdu	1076	17	and	and	CC
cord-014597-66vd2mdu	1076	18	lysosomes	lysosome	NNS
cord-014597-66vd2mdu	1076	19	.	.	.
cord-014597-66vd2mdu	1077	1	SEC	SEC	NNP
cord-014597-66vd2mdu	1077	2	(	(	-LRB-
cord-014597-66vd2mdu	1077	3	Fig	Fig	NNP
cord-014597-66vd2mdu	1077	4	.	.	.
cord-014597-66vd2mdu	1077	5	1e	1e	LS
cord-014597-66vd2mdu	1077	6	)	)	-RRB-
cord-014597-66vd2mdu	1078	1	analyses	analysis	NNS
cord-014597-66vd2mdu	1078	2	showed	show	VBD
cord-014597-66vd2mdu	1078	3	differences	difference	NNS
cord-014597-66vd2mdu	1078	4	between	between	IN
cord-014597-66vd2mdu	1078	5	the	the	DT
cord-014597-66vd2mdu	1078	6	tested	test	VBN
cord-014597-66vd2mdu	1078	7	poloxamers	poloxamer	NNS
cord-014597-66vd2mdu	1078	8	.	.	.
cord-014597-66vd2mdu	1079	1	Especially	especially	RB
cord-014597-66vd2mdu	1079	2	tested	test	VBN
cord-014597-66vd2mdu	1079	3	lots	lot	NNS
cord-014597-66vd2mdu	1079	4	of	of	IN
cord-014597-66vd2mdu	1079	5	Pluronic	Pluronic	NNP
cord-014597-66vd2mdu	1080	1	®	®	,
cord-014597-66vd2mdu	1080	2	F-68	F-68	NNP
cord-014597-66vd2mdu	1080	3	revealed	reveal	VBD
cord-014597-66vd2mdu	1080	4	notable	notable	JJ
cord-014597-66vd2mdu	1080	5	deviations	deviation	NNS
cord-014597-66vd2mdu	1080	6	in	in	IN
cord-014597-66vd2mdu	1080	7	the	the	DT
cord-014597-66vd2mdu	1080	8	low	low	JJ
cord-014597-66vd2mdu	1080	9	molecular	molecular	JJ
cord-014597-66vd2mdu	1080	10	weight	weight	NN
cord-014597-66vd2mdu	1080	11	fraction	fraction	NN
cord-014597-66vd2mdu	1080	12	(	(	-LRB-
cord-014597-66vd2mdu	1080	13	peak	peak	NNP
cord-014597-66vd2mdu	1080	14	2	2	CD
cord-014597-66vd2mdu	1080	15	,	,	,
cord-014597-66vd2mdu	1080	16	Fig	Fig	NNP
cord-014597-66vd2mdu	1080	17	.	.	.
cord-014597-66vd2mdu	1080	18	1e	1e	NNP
cord-014597-66vd2mdu	1080	19	)	)	-RRB-
cord-014597-66vd2mdu	1080	20	,	,	,
cord-014597-66vd2mdu	1080	21	compared	compare	VBN
cord-014597-66vd2mdu	1080	22	to	to	IN
cord-014597-66vd2mdu	1080	23	the	the	DT
cord-014597-66vd2mdu	1080	24	other	other	JJ
cord-014597-66vd2mdu	1080	25	poloxamers	poloxamer	NNS
cord-014597-66vd2mdu	1080	26	.	.	.
cord-014597-66vd2mdu	1081	1	Cultures	culture	NNS
cord-014597-66vd2mdu	1081	2	subjected	subject	VBN
cord-014597-66vd2mdu	1081	3	to	to	IN
cord-014597-66vd2mdu	1081	4	varying	vary	VBG
cord-014597-66vd2mdu	1081	5	levels	level	NNS
cord-014597-66vd2mdu	1081	6	of	of	IN
cord-014597-66vd2mdu	1081	7	shear	shear	NN
cord-014597-66vd2mdu	1081	8	stress	stress	NN
cord-014597-66vd2mdu	1081	9	showed	show	VBD
cord-014597-66vd2mdu	1081	10	distinct	distinct	JJ
cord-014597-66vd2mdu	1081	11	growth	growth	NN
cord-014597-66vd2mdu	1081	12	differences	difference	NNS
cord-014597-66vd2mdu	1081	13	depending	depend	VBG
cord-014597-66vd2mdu	1081	14	on	on	IN
cord-014597-66vd2mdu	1081	15	used	use	VBN
cord-014597-66vd2mdu	1081	16	poloxamer	poloxamer	NN
cord-014597-66vd2mdu	1081	17	.	.	.
cord-014597-66vd2mdu	1082	1	While	while	IN
cord-014597-66vd2mdu	1082	2	experiments	experiment	NNS
cord-014597-66vd2mdu	1082	3	in	in	IN
cord-014597-66vd2mdu	1082	4	plain	plain	JJ
cord-014597-66vd2mdu	1082	5	shake	shake	NN
cord-014597-66vd2mdu	1082	6	flasks	flask	NNS
cord-014597-66vd2mdu	1082	7	did	do	VBD
cord-014597-66vd2mdu	1082	8	not	not	RB
cord-014597-66vd2mdu	1082	9	show	show	VB
cord-014597-66vd2mdu	1082	10	any	any	DT
cord-014597-66vd2mdu	1082	11	differences	difference	NNS
cord-014597-66vd2mdu	1082	12	in	in	IN
cord-014597-66vd2mdu	1082	13	growth	growth	NN
cord-014597-66vd2mdu	1082	14	,	,	,
cord-014597-66vd2mdu	1082	15	cultivations	cultivation	NNS
cord-014597-66vd2mdu	1082	16	under	under	IN
cord-014597-66vd2mdu	1082	17	elevated	elevated	JJ
cord-014597-66vd2mdu	1082	18	shear	shear	NN
cord-014597-66vd2mdu	1082	19	stress	stress	NN
cord-014597-66vd2mdu	1082	20	in	in	IN
cord-014597-66vd2mdu	1082	21	baffled	baffled	JJ
cord-014597-66vd2mdu	1082	22	shake	shake	NN
cord-014597-66vd2mdu	1082	23	flasks	flask	NNS
cord-014597-66vd2mdu	1082	24	resulted	result	VBD
cord-014597-66vd2mdu	1082	25	in	in	IN
cord-014597-66vd2mdu	1082	26	lower	low	JJR
cord-014597-66vd2mdu	1082	27	peak	peak	JJ
cord-014597-66vd2mdu	1082	28	viable	viable	JJ
cord-014597-66vd2mdu	1082	29	cell	cell	NN
cord-014597-66vd2mdu	1082	30	densities	density	NNS
cord-014597-66vd2mdu	1082	31	with	with	IN
cord-014597-66vd2mdu	1082	32	Kolliphor	Kolliphor	NNP
cord-014597-66vd2mdu	1083	1	®	®	NNP
cord-014597-66vd2mdu	1083	2	P188	P188	NNP
cord-014597-66vd2mdu	1083	3	and	and	CC
cord-014597-66vd2mdu	1083	4	some	some	DT
cord-014597-66vd2mdu	1083	5	Pluronic	Pluronic	NNP
cord-014597-66vd2mdu	1084	1	®	®	.
cord-014597-66vd2mdu	1085	1	F-68	F-68	NNP
cord-014597-66vd2mdu	1085	2	lots	lot	NNS
cord-014597-66vd2mdu	1085	3	.	.	.
cord-014597-66vd2mdu	1086	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1086	2	remaines	remain	VBZ
cord-014597-66vd2mdu	1086	3	unclear	unclear	JJ
cord-014597-66vd2mdu	1086	4	whether	whether	IN
cord-014597-66vd2mdu	1086	5	this	this	DT
cord-014597-66vd2mdu	1086	6	can	can	MD
cord-014597-66vd2mdu	1086	7	be	be	VB
cord-014597-66vd2mdu	1086	8	explained	explain	VBN
cord-014597-66vd2mdu	1086	9	by	by	IN
cord-014597-66vd2mdu	1086	10	different	different	JJ
cord-014597-66vd2mdu	1086	11	membrane	membrane	NN
cord-014597-66vd2mdu	1086	12	protective	protective	JJ
cord-014597-66vd2mdu	1086	13	activities	activity	NNS
cord-014597-66vd2mdu	1086	14	alone	alone	RB
cord-014597-66vd2mdu	1086	15	,	,	,
cord-014597-66vd2mdu	1086	16	or	or	CC
cord-014597-66vd2mdu	1086	17	if	if	IN
cord-014597-66vd2mdu	1086	18	other	other	JJ
cord-014597-66vd2mdu	1086	19	mechanisms	mechanism	NNS
cord-014597-66vd2mdu	1086	20	,	,	,
cord-014597-66vd2mdu	1086	21	occuring	occur	VBG
cord-014597-66vd2mdu	1086	22	during	during	IN
cord-014597-66vd2mdu	1086	23	and	and	CC
cord-014597-66vd2mdu	1086	24	after	after	IN
cord-014597-66vd2mdu	1086	25	cellular	cellular	JJ
cord-014597-66vd2mdu	1086	26	uptake	uptake	NN
cord-014597-66vd2mdu	1086	27	,	,	,
cord-014597-66vd2mdu	1086	28	contribute	contribute	VBP
cord-014597-66vd2mdu	1086	29	to	to	IN
cord-014597-66vd2mdu	1086	30	this	this	DT
cord-014597-66vd2mdu	1086	31	effect	effect	NN
cord-014597-66vd2mdu	1086	32	.	.	.
cord-014597-66vd2mdu	1087	1	Especially	especially	RB
cord-014597-66vd2mdu	1087	2	for	for	IN
cord-014597-66vd2mdu	1087	3	the	the	DT
cord-014597-66vd2mdu	1087	4	tested	test	VBN
cord-014597-66vd2mdu	1087	5	lots	lot	NNS
cord-014597-66vd2mdu	1087	6	of	of	IN
cord-014597-66vd2mdu	1087	7	Pluronic	Pluronic	NNP
cord-014597-66vd2mdu	1087	8	®	®	.
cord-014597-66vd2mdu	1087	9	F-68	F-68	NNP
cord-014597-66vd2mdu	1087	10	,	,	,
cord-014597-66vd2mdu	1087	11	SEC	SEC	NNP
cord-014597-66vd2mdu	1087	12	of	of	IN
cord-014597-66vd2mdu	1087	13	surfactants	surfactant	NNS
cord-014597-66vd2mdu	1087	14	showed	show	VBD
cord-014597-66vd2mdu	1087	15	differences	difference	NNS
cord-014597-66vd2mdu	1087	16	in	in	IN
cord-014597-66vd2mdu	1087	17	the	the	DT
cord-014597-66vd2mdu	1087	18	low	low	JJ
cord-014597-66vd2mdu	1087	19	molecular	molecular	JJ
cord-014597-66vd2mdu	1087	20	weight	weight	NN
cord-014597-66vd2mdu	1087	21	fraction	fraction	NN
cord-014597-66vd2mdu	1087	22	.	.	.
cord-014597-66vd2mdu	1088	1	This	this	DT
cord-014597-66vd2mdu	1088	2	fraction	fraction	NN
cord-014597-66vd2mdu	1088	3	mainly	mainly	RB
cord-014597-66vd2mdu	1088	4	represents	represent	VBZ
cord-014597-66vd2mdu	1088	5	polyethylen	polyethylen	NN
cord-014597-66vd2mdu	1088	6	oxide	oxide	NN
cord-014597-66vd2mdu	1088	7	(	(	-LRB-
cord-014597-66vd2mdu	1088	8	PEO	PEO	NNP
cord-014597-66vd2mdu	1088	9	)	)	-RRB-
cord-014597-66vd2mdu	1088	10	(	(	-LRB-
cord-014597-66vd2mdu	1088	11	revealed	reveal	VBN
cord-014597-66vd2mdu	1088	12	by	by	IN
cord-014597-66vd2mdu	1088	13	NMR	NMR	NNP
cord-014597-66vd2mdu	1088	14	)	)	-RRB-
cord-014597-66vd2mdu	1088	15	,	,	,
cord-014597-66vd2mdu	1088	16	which	which	WDT
cord-014597-66vd2mdu	1088	17	is	be	VBZ
cord-014597-66vd2mdu	1088	18	likely	likely	JJ
cord-014597-66vd2mdu	1088	19	to	to	TO
cord-014597-66vd2mdu	1088	20	be	be	VB
cord-014597-66vd2mdu	1088	21	a	a	DT
cord-014597-66vd2mdu	1088	22	remnant	remnant	NN
cord-014597-66vd2mdu	1088	23	from	from	IN
cord-014597-66vd2mdu	1088	24	synthesis	synthesis	NN
cord-014597-66vd2mdu	1088	25	.	.	.
cord-014597-66vd2mdu	1089	1	These	these	DT
cord-014597-66vd2mdu	1089	2	observations	observation	NNS
cord-014597-66vd2mdu	1089	3	indicate	indicate	VBP
cord-014597-66vd2mdu	1089	4	that	that	IN
cord-014597-66vd2mdu	1089	5	the	the	DT
cord-014597-66vd2mdu	1089	6	use	use	NN
cord-014597-66vd2mdu	1089	7	of	of	IN
cord-014597-66vd2mdu	1089	8	different	different	JJ
cord-014597-66vd2mdu	1089	9	poloxamers	poloxamer	NNS
cord-014597-66vd2mdu	1089	10	and	and	CC
cord-014597-66vd2mdu	1089	11	lots	lot	NNS
cord-014597-66vd2mdu	1089	12	thereof	thereof	RB
cord-014597-66vd2mdu	1089	13	should	should	MD
cord-014597-66vd2mdu	1089	14	be	be	VB
cord-014597-66vd2mdu	1089	15	carefully	carefully	RB
cord-014597-66vd2mdu	1089	16	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1089	17	,	,	,
cord-014597-66vd2mdu	1089	18	especially	especially	RB
cord-014597-66vd2mdu	1089	19	under	under	IN
cord-014597-66vd2mdu	1089	20	elevated	elevated	JJ
cord-014597-66vd2mdu	1089	21	shear	shear	NN
cord-014597-66vd2mdu	1089	22	stress	stress	NN
cord-014597-66vd2mdu	1089	23	.	.	.
cord-014597-66vd2mdu	1090	1	Further	further	JJ
cord-014597-66vd2mdu	1090	2	experiments	experiment	NNS
cord-014597-66vd2mdu	1090	3	will	will	MD
cord-014597-66vd2mdu	1090	4	focus	focus	VB
cord-014597-66vd2mdu	1090	5	on	on	IN
cord-014597-66vd2mdu	1090	6	investigating	investigate	VBG
cord-014597-66vd2mdu	1090	7	distinct	distinct	JJ
cord-014597-66vd2mdu	1090	8	SEC	SEC	NNP
cord-014597-66vd2mdu	1090	9	fractions	fraction	NNS
cord-014597-66vd2mdu	1090	10	of	of	IN
cord-014597-66vd2mdu	1090	11	poloxamers	poloxamer	NNS
cord-014597-66vd2mdu	1090	12	.	.	.
cord-014597-66vd2mdu	1091	1	Overcoming	overcome	VBG
cord-014597-66vd2mdu	1091	2	(	(	-LRB-
cord-014597-66vd2mdu	1091	3	Fig	Fig	NNP
cord-014597-66vd2mdu	1091	4	.	.	.
cord-014597-66vd2mdu	1091	5	1	1	CD
cord-014597-66vd2mdu	1091	6	)	)	-RRB-
cord-014597-66vd2mdu	1091	7	.	.	.
cord-014597-66vd2mdu	1092	1	Aurintricarboxylic	Aurintricarboxylic	NNP
cord-014597-66vd2mdu	1092	2	acid	acid	NN
cord-014597-66vd2mdu	1092	3	(	(	-LRB-
cord-014597-66vd2mdu	1092	4	endonuclease	endonuclease	NN
cord-014597-66vd2mdu	1092	5	inhibitor	inhibitor	NN
cord-014597-66vd2mdu	1092	6	;	;	:
cord-014597-66vd2mdu	1092	7	enhancer	enhancer	NN
cord-014597-66vd2mdu	1092	8	used	use	VBN
cord-014597-66vd2mdu	1092	9	in	in	IN
cord-014597-66vd2mdu	1092	10	e.g.	e.g.	JJ
cord-014597-66vd2mdu	1092	11	salivary	salivary	NN
cord-014597-66vd2mdu	1092	12	gland	gland	NN
cord-014597-66vd2mdu	1092	13	transfection	transfection	NN
cord-014597-66vd2mdu	1092	14	)	)	-RRB-
cord-014597-66vd2mdu	1092	15	and	and	CC
cord-014597-66vd2mdu	1092	16	polyvinylpyrrolidone	polyvinylpyrrolidone	NNP
cord-014597-66vd2mdu	1092	17	(	(	-LRB-
cord-014597-66vd2mdu	1092	18	polymer	polymer	NN
cord-014597-66vd2mdu	1092	19	;	;	,
cord-014597-66vd2mdu	1092	20	beneficial	beneficial	JJ
cord-014597-66vd2mdu	1092	21	in	in	IN
cord-014597-66vd2mdu	1092	22	electroporation	electroporation	NN
cord-014597-66vd2mdu	1092	23	)	)	-RRB-
cord-014597-66vd2mdu	1092	24	were	be	VBD
cord-014597-66vd2mdu	1092	25	both	both	DT
cord-014597-66vd2mdu	1092	26	found	find	VBN
cord-014597-66vd2mdu	1092	27	to	to	TO
cord-014597-66vd2mdu	1092	28	negatively	negatively	RB
cord-014597-66vd2mdu	1092	29	impact	impact	VB
cord-014597-66vd2mdu	1092	30	PEImediated	peimediate	VBN
cord-014597-66vd2mdu	1092	31	transfection	transfection	NN
cord-014597-66vd2mdu	1092	32	of	of	IN
cord-014597-66vd2mdu	1092	33	CHO	CHO	NNP
cord-014597-66vd2mdu	1092	34	cells	cell	NNS
cord-014597-66vd2mdu	1092	35	,	,	,
cord-014597-66vd2mdu	1092	36	while	while	IN
cord-014597-66vd2mdu	1092	37	another	another	DT
cord-014597-66vd2mdu	1092	38	tested	test	VBN
cord-014597-66vd2mdu	1092	39	polymer	polymer	NN
cord-014597-66vd2mdu	1092	40	enhanced	enhance	VBN
cord-014597-66vd2mdu	1092	41	growth	growth	NN
cord-014597-66vd2mdu	1092	42	as	as	RB
cord-014597-66vd2mdu	1092	43	well	well	RB
cord-014597-66vd2mdu	1092	44	as	as	IN
cord-014597-66vd2mdu	1092	45	transfection	transfection	NN
cord-014597-66vd2mdu	1092	46	efficiency	efficiency	NN
cord-014597-66vd2mdu	1092	47	.	.	.
cord-014597-66vd2mdu	1093	1	The	the	DT
cord-014597-66vd2mdu	1093	2	use	use	NN
cord-014597-66vd2mdu	1093	3	of	of	IN
cord-014597-66vd2mdu	1093	4	a	a	DT
cord-014597-66vd2mdu	1093	5	strong	strong	JJ
cord-014597-66vd2mdu	1093	6	chelator	chelator	NN
cord-014597-66vd2mdu	1093	7	led	lead	VBD
cord-014597-66vd2mdu	1093	8	to	to	IN
cord-014597-66vd2mdu	1093	9	a	a	DT
cord-014597-66vd2mdu	1093	10	high	high	JJ
cord-014597-66vd2mdu	1093	11	transfection	transfection	NN
cord-014597-66vd2mdu	1093	12	efficiency	efficiency	NN
cord-014597-66vd2mdu	1093	13	,	,	,
cord-014597-66vd2mdu	1093	14	but	but	CC
cord-014597-66vd2mdu	1093	15	impaired	impaired	JJ
cord-014597-66vd2mdu	1093	16	cell	cell	NN
cord-014597-66vd2mdu	1093	17	growth	growth	NN
cord-014597-66vd2mdu	1093	18	.	.	.
cord-014597-66vd2mdu	1094	1	Based	base	VBN
cord-014597-66vd2mdu	1094	2	on	on	IN
cord-014597-66vd2mdu	1094	3	the	the	DT
cord-014597-66vd2mdu	1094	4	results	result	NNS
cord-014597-66vd2mdu	1094	5	of	of	IN
cord-014597-66vd2mdu	1094	6	the	the	DT
cord-014597-66vd2mdu	1094	7	independent	independent	JJ
cord-014597-66vd2mdu	1094	8	substance	substance	NN
cord-014597-66vd2mdu	1094	9	testings	testing	NNS
cord-014597-66vd2mdu	1094	10	,	,	,
cord-014597-66vd2mdu	1094	11	the	the	DT
cord-014597-66vd2mdu	1094	12	medium	medium	JJ
cord-014597-66vd2mdu	1094	13	formulation	formulation	NN
cord-014597-66vd2mdu	1094	14	was	be	VBD
cord-014597-66vd2mdu	1094	15	modified	modify	VBN
cord-014597-66vd2mdu	1094	16	by	by	IN
cord-014597-66vd2mdu	1094	17	the	the	DT
cord-014597-66vd2mdu	1094	18	addition	addition	NN
cord-014597-66vd2mdu	1094	19	of	of	IN
cord-014597-66vd2mdu	1094	20	a	a	DT
cord-014597-66vd2mdu	1094	21	weak	weak	JJ
cord-014597-66vd2mdu	1094	22	chelator	chelator	NN
cord-014597-66vd2mdu	1094	23	and	and	CC
cord-014597-66vd2mdu	1094	24	further	further	JJ
cord-014597-66vd2mdu	1094	25	components	component	NNS
cord-014597-66vd2mdu	1094	26	including	include	VBG
cord-014597-66vd2mdu	1094	27	vitamins	vitamin	NNS
cord-014597-66vd2mdu	1094	28	.	.	.
cord-014597-66vd2mdu	1095	1	Different	different	JJ
cord-014597-66vd2mdu	1095	2	osmolalities	osmolalitie	NNS
cord-014597-66vd2mdu	1095	3	between	between	IN
cord-014597-66vd2mdu	1095	4	280	280	CD
cord-014597-66vd2mdu	1095	5	mOsmol	mOsmol	NNP
cord-014597-66vd2mdu	1095	6	/	/	SYM
cord-014597-66vd2mdu	1095	7	kg	kg	NN
cord-014597-66vd2mdu	1095	8	and	and	CC
cord-014597-66vd2mdu	1095	9	340	340	CD
cord-014597-66vd2mdu	1095	10	mOsmol	mOsmol	NNP
cord-014597-66vd2mdu	1095	11	/	/	SYM
cord-014597-66vd2mdu	1095	12	kg	kg	NNS
cord-014597-66vd2mdu	1095	13	were	be	VBD
cord-014597-66vd2mdu	1095	14	tested	test	VBN
cord-014597-66vd2mdu	1095	15	for	for	IN
cord-014597-66vd2mdu	1095	16	the	the	DT
cord-014597-66vd2mdu	1095	17	final	final	JJ
cord-014597-66vd2mdu	1095	18	formulation	formulation	NN
cord-014597-66vd2mdu	1095	19	,	,	,
cord-014597-66vd2mdu	1095	20	but	but	CC
cord-014597-66vd2mdu	1095	21	no	no	DT
cord-014597-66vd2mdu	1095	22	major	major	JJ
cord-014597-66vd2mdu	1095	23	impact	impact	NN
cord-014597-66vd2mdu	1095	24	was	be	VBD
cord-014597-66vd2mdu	1095	25	seen	see	VBN
cord-014597-66vd2mdu	1095	26	neither	neither	CC
cord-014597-66vd2mdu	1095	27	on	on	IN
cord-014597-66vd2mdu	1095	28	transfection	transfection	NN
cord-014597-66vd2mdu	1095	29	efficiency	efficiency	NN
cord-014597-66vd2mdu	1095	30	nor	nor	CC
cord-014597-66vd2mdu	1095	31	on	on	IN
cord-014597-66vd2mdu	1095	32	viability	viability	NN
cord-014597-66vd2mdu	1095	33	2	2	CD
cord-014597-66vd2mdu	1095	34	days	day	NNS
cord-014597-66vd2mdu	1095	35	post	post	NN
cord-014597-66vd2mdu	1095	36	-	-	NN
cord-014597-66vd2mdu	1095	37	transfection	transfection	NN
cord-014597-66vd2mdu	1095	38	.	.	.
cord-014597-66vd2mdu	1096	1	The	the	DT
cord-014597-66vd2mdu	1096	2	final	final	JJ
cord-014597-66vd2mdu	1096	3	CHO	CHO	NNP
cord-014597-66vd2mdu	1096	4	TF	TF	NNP
cord-014597-66vd2mdu	1096	5	medium	medium	NN
cord-014597-66vd2mdu	1096	6	formulation	formulation	NN
cord-014597-66vd2mdu	1096	7	supported	support	VBD
cord-014597-66vd2mdu	1096	8	high	high	JJ
cord-014597-66vd2mdu	1096	9	cell	cell	NN
cord-014597-66vd2mdu	1096	10	growth	growth	NN
cord-014597-66vd2mdu	1096	11	of	of	IN
cord-014597-66vd2mdu	1096	12	finally	finally	RB
cord-014597-66vd2mdu	1096	13	tested	test	VBN
cord-014597-66vd2mdu	1096	14	CHO	CHO	NNP
cord-014597-66vd2mdu	1096	15	cell	cell	NN
cord-014597-66vd2mdu	1096	16	lines	line	NNS
cord-014597-66vd2mdu	1096	17	2	2	CD
cord-014597-66vd2mdu	1096	18	and	and	CC
cord-014597-66vd2mdu	1096	19	3	3	CD
cord-014597-66vd2mdu	1096	20	with	with	IN
cord-014597-66vd2mdu	1096	21	peak	peak	JJ
cord-014597-66vd2mdu	1096	22	viable	viable	JJ
cord-014597-66vd2mdu	1096	23	cell	cell	NN
cord-014597-66vd2mdu	1096	24	densities	density	NNS
cord-014597-66vd2mdu	1096	25	above	above	IN
cord-014597-66vd2mdu	1096	26	10⋅10	10⋅10	CD
cord-014597-66vd2mdu	1096	27	6	6	CD
cord-014597-66vd2mdu	1096	28	cells	cell	NNS
cord-014597-66vd2mdu	1096	29	/	/	SYM
cord-014597-66vd2mdu	1096	30	ml	ml	NNS
cord-014597-66vd2mdu	1096	31	in	in	IN
cord-014597-66vd2mdu	1096	32	batch	batch	NN
cord-014597-66vd2mdu	1096	33	cultivations	cultivation	NNS
cord-014597-66vd2mdu	1096	34	with	with	IN
cord-014597-66vd2mdu	1096	35	an	an	DT
cord-014597-66vd2mdu	1096	36	overall	overall	JJ
cord-014597-66vd2mdu	1096	37	cultivation	cultivation	NN
cord-014597-66vd2mdu	1096	38	time	time	NN
cord-014597-66vd2mdu	1096	39	of	of	IN
cord-014597-66vd2mdu	1096	40	7	7	CD
cord-014597-66vd2mdu	1096	41	-	-	SYM
cord-014597-66vd2mdu	1096	42	8	8	CD
cord-014597-66vd2mdu	1096	43	days	day	NNS
cord-014597-66vd2mdu	1096	44	(	(	-LRB-
cord-014597-66vd2mdu	1096	45	Fig	fig	NN
cord-014597-66vd2mdu	1096	46	.	.	.
cord-014597-66vd2mdu	1096	47	1	1	CD
cord-014597-66vd2mdu	1096	48	)	)	-RRB-
cord-014597-66vd2mdu	1096	49	.	.	.
cord-014597-66vd2mdu	1097	1	Further	further	JJ
cord-014597-66vd2mdu	1097	2	improvements	improvement	NNS
cord-014597-66vd2mdu	1097	3	of	of	IN
cord-014597-66vd2mdu	1097	4	the	the	DT
cord-014597-66vd2mdu	1097	5	process	process	NN
cord-014597-66vd2mdu	1097	6	might	may	MD
cord-014597-66vd2mdu	1097	7	be	be	VB
cord-014597-66vd2mdu	1097	8	achieved	achieve	VBN
cord-014597-66vd2mdu	1097	9	by	by	IN
cord-014597-66vd2mdu	1097	10	adapting	adapt	VBG
cord-014597-66vd2mdu	1097	11	the	the	DT
cord-014597-66vd2mdu	1097	12	protocol	protocol	NN
cord-014597-66vd2mdu	1097	13	,	,	,
cord-014597-66vd2mdu	1097	14	as	as	IN
cord-014597-66vd2mdu	1097	15	the	the	DT
cord-014597-66vd2mdu	1097	16	results	result	NNS
cord-014597-66vd2mdu	1097	17	shown	show	VBN
cord-014597-66vd2mdu	1097	18	are	be	VBP
cord-014597-66vd2mdu	1097	19	based	base	VBN
cord-014597-66vd2mdu	1097	20	on	on	IN
cord-014597-66vd2mdu	1097	21	a	a	DT
cord-014597-66vd2mdu	1097	22	simple	simple	JJ
cord-014597-66vd2mdu	1097	23	precomplexing	precomplexing	NN
cord-014597-66vd2mdu	1097	24	of	of	IN
cord-014597-66vd2mdu	1097	25	DNA	DNA	NNP
cord-014597-66vd2mdu	1097	26	-	-	HYPH
cord-014597-66vd2mdu	1097	27	PEI	PEI	NNP
cord-014597-66vd2mdu	1097	28	.	.	.
cord-014597-66vd2mdu	1098	1	Moreover	moreover	RB
cord-014597-66vd2mdu	1098	2	,	,	,
cord-014597-66vd2mdu	1098	3	product	product	NN
cord-014597-66vd2mdu	1098	4	yields	yield	NNS
cord-014597-66vd2mdu	1098	5	could	could	MD
cord-014597-66vd2mdu	1098	6	potentially	potentially	RB
cord-014597-66vd2mdu	1098	7	be	be	VB
cord-014597-66vd2mdu	1098	8	increased	increase	VBN
cord-014597-66vd2mdu	1098	9	by	by	IN
cord-014597-66vd2mdu	1098	10	using	use	VBG
cord-014597-66vd2mdu	1098	11	feeds	feed	NNS
cord-014597-66vd2mdu	1098	12	,	,	,
cord-014597-66vd2mdu	1098	13	temperature	temperature	NN
cord-014597-66vd2mdu	1098	14	shifts	shift	NNS
cord-014597-66vd2mdu	1098	15	or	or	CC
cord-014597-66vd2mdu	1098	16	commonly	commonly	RB
cord-014597-66vd2mdu	1098	17	used	use	VBN
cord-014597-66vd2mdu	1098	18	enhancers	enhancer	NNS
cord-014597-66vd2mdu	1098	19	(	(	-LRB-
cord-014597-66vd2mdu	1098	20	e.g.	e.g.	RB
cord-014597-66vd2mdu	1098	21	valproic	valproic	JJ
cord-014597-66vd2mdu	1098	22	acids	acid	NNS
cord-014597-66vd2mdu	1098	23	)	)	-RRB-
cord-014597-66vd2mdu	1098	24	.	.	.
cord-014597-66vd2mdu	1099	1	Scaling	scale	VBG
cord-014597-66vd2mdu	1099	2	of	of	IN
cord-014597-66vd2mdu	1099	3	a	a	DT
cord-014597-66vd2mdu	1099	4	cell	cell	NN
cord-014597-66vd2mdu	1099	5	culture	culture	NN
cord-014597-66vd2mdu	1099	6	process	process	NN
cord-014597-66vd2mdu	1099	7	is	be	VBZ
cord-014597-66vd2mdu	1099	8	an	an	DT
cord-014597-66vd2mdu	1099	9	essential	essential	JJ
cord-014597-66vd2mdu	1099	10	part	part	NN
cord-014597-66vd2mdu	1099	11	in	in	IN
cord-014597-66vd2mdu	1099	12	its	-PRON-	PRP$
cord-014597-66vd2mdu	1099	13	development	development	NN
cord-014597-66vd2mdu	1099	14	.	.	.
cord-014597-66vd2mdu	1100	1	In	in	IN
cord-014597-66vd2mdu	1100	2	a	a	DT
cord-014597-66vd2mdu	1100	3	typical	typical	JJ
cord-014597-66vd2mdu	1100	4	approach	approach	NN
cord-014597-66vd2mdu	1100	5	scaling	scale	VBG
cord-014597-66vd2mdu	1100	6	[	[	-LRB-
cord-014597-66vd2mdu	1100	7	1	1	CD
cord-014597-66vd2mdu	1100	8	]	]	-RRB-
cord-014597-66vd2mdu	1100	9	is	be	VBZ
cord-014597-66vd2mdu	1100	10	performed	perform	VBN
cord-014597-66vd2mdu	1100	11	by	by	IN
cord-014597-66vd2mdu	1100	12	keeping	keep	VBG
cord-014597-66vd2mdu	1100	13	a	a	DT
cord-014597-66vd2mdu	1100	14	(	(	-LRB-
cord-014597-66vd2mdu	1100	15	critical	critical	JJ
cord-014597-66vd2mdu	1100	16	)	)	-RRB-
cord-014597-66vd2mdu	1100	17	process	process	NN
cord-014597-66vd2mdu	1100	18	parameter	parameter	NN
cord-014597-66vd2mdu	1100	19	constant	constant	JJ
cord-014597-66vd2mdu	1100	20	throughout	throughout	IN
cord-014597-66vd2mdu	1100	21	the	the	DT
cord-014597-66vd2mdu	1100	22	complete	complete	JJ
cord-014597-66vd2mdu	1100	23	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1100	24	range	range	NN
cord-014597-66vd2mdu	1100	25	.	.	.
cord-014597-66vd2mdu	1101	1	This	this	DT
cord-014597-66vd2mdu	1101	2	can	can	MD
cord-014597-66vd2mdu	1101	3	lead	lead	VB
cord-014597-66vd2mdu	1101	4	to	to	IN
cord-014597-66vd2mdu	1101	5	non	non	JJ
cord-014597-66vd2mdu	1101	6	-	-	JJ
cord-014597-66vd2mdu	1101	7	beneficial	beneficial	JJ
cord-014597-66vd2mdu	1101	8	results	result	NNS
cord-014597-66vd2mdu	1101	9	either	either	CC
cord-014597-66vd2mdu	1101	10	on	on	IN
cord-014597-66vd2mdu	1101	11	the	the	DT
cord-014597-66vd2mdu	1101	12	high	high	JJ
cord-014597-66vd2mdu	1101	13	or	or	CC
cord-014597-66vd2mdu	1101	14	the	the	DT
cord-014597-66vd2mdu	1101	15	low	low	JJ
cord-014597-66vd2mdu	1101	16	end	end	NN
cord-014597-66vd2mdu	1101	17	of	of	IN
cord-014597-66vd2mdu	1101	18	the	the	DT
cord-014597-66vd2mdu	1101	19	range	range	NN
cord-014597-66vd2mdu	1101	20	.	.	.
cord-014597-66vd2mdu	1102	1	For	for	IN
cord-014597-66vd2mdu	1102	2	instance	instance	NN
cord-014597-66vd2mdu	1102	3	,	,	,
cord-014597-66vd2mdu	1102	4	the	the	DT
cord-014597-66vd2mdu	1102	5	specific	specific	JJ
cord-014597-66vd2mdu	1102	6	power	power	NN
cord-014597-66vd2mdu	1102	7	input	input	NN
cord-014597-66vd2mdu	1102	8	[	[	-LRB-
cord-014597-66vd2mdu	1102	9	P	p	NN
cord-014597-66vd2mdu	1102	10	/	/	SYM
cord-014597-66vd2mdu	1102	11	V	v	NN
cord-014597-66vd2mdu	1102	12	]	]	-RRB-
cord-014597-66vd2mdu	1102	13	of	of	IN
cord-014597-66vd2mdu	1102	14	30	30	CD
cord-014597-66vd2mdu	1102	15	W	W	NNP
cord-014597-66vd2mdu	1102	16	/	/	SYM
cord-014597-66vd2mdu	1102	17	m	m	NN
cord-014597-66vd2mdu	1102	18	3	3	CD
cord-014597-66vd2mdu	1102	19	might	may	MD
cord-014597-66vd2mdu	1102	20	result	result	VB
cord-014597-66vd2mdu	1102	21	in	in	IN
cord-014597-66vd2mdu	1102	22	a	a	DT
cord-014597-66vd2mdu	1102	23	good	good	JJ
cord-014597-66vd2mdu	1102	24	agitation	agitation	NN
cord-014597-66vd2mdu	1102	25	in	in	IN
cord-014597-66vd2mdu	1102	26	production	production	NN
cord-014597-66vd2mdu	1102	27	scale	scale	NN
cord-014597-66vd2mdu	1102	28	whereas	whereas	IN
cord-014597-66vd2mdu	1102	29	it	-PRON-	PRP
cord-014597-66vd2mdu	1102	30	leads	lead	VBZ
cord-014597-66vd2mdu	1102	31	to	to	IN
cord-014597-66vd2mdu	1102	32	a	a	DT
cord-014597-66vd2mdu	1102	33	nonturbulent	nonturbulent	NN
cord-014597-66vd2mdu	1102	34	mixing	mix	VBG
cord-014597-66vd2mdu	1102	35	behavior	behavior	NN
cord-014597-66vd2mdu	1102	36	in	in	IN
cord-014597-66vd2mdu	1102	37	process	process	NN
cord-014597-66vd2mdu	1102	38	development	development	NN
cord-014597-66vd2mdu	1102	39	scale	scale	NN
cord-014597-66vd2mdu	1102	40	.	.	.
cord-014597-66vd2mdu	1103	1	To	to	TO
cord-014597-66vd2mdu	1103	2	overcome	overcome	VB
cord-014597-66vd2mdu	1103	3	this	this	DT
cord-014597-66vd2mdu	1103	4	issue	issue	NN
cord-014597-66vd2mdu	1103	5	a	a	DT
cord-014597-66vd2mdu	1103	6	new	new	JJ
cord-014597-66vd2mdu	1103	7	approach	approach	NN
cord-014597-66vd2mdu	1103	8	for	for	IN
cord-014597-66vd2mdu	1103	9	an	an	DT
cord-014597-66vd2mdu	1103	10	easy	easy	JJ
cord-014597-66vd2mdu	1103	11	scaling	scaling	NN
cord-014597-66vd2mdu	1103	12	procedure	procedure	NN
cord-014597-66vd2mdu	1103	13	was	be	VBD
cord-014597-66vd2mdu	1103	14	developed	develop	VBN
cord-014597-66vd2mdu	1103	15	.	.	.
cord-014597-66vd2mdu	1104	1	This	this	DT
cord-014597-66vd2mdu	1104	2	"	"	``
cord-014597-66vd2mdu	1104	3	Utility	Utility	NNP
cord-014597-66vd2mdu	1104	4	Function	function	NN
cord-014597-66vd2mdu	1104	5	"	"	''
cord-014597-66vd2mdu	1104	6	approach	approach	NN
cord-014597-66vd2mdu	1104	7	for	for	IN
cord-014597-66vd2mdu	1104	8	agitation	agitation	NN
cord-014597-66vd2mdu	1104	9	scaling	scaling	NN
cord-014597-66vd2mdu	1104	10	is	be	VBZ
cord-014597-66vd2mdu	1104	11	based	base	VBN
cord-014597-66vd2mdu	1104	12	on	on	IN
cord-014597-66vd2mdu	1104	13	individual	individual	JJ
cord-014597-66vd2mdu	1104	14	functions	function	NNS
cord-014597-66vd2mdu	1104	15	with	with	IN
cord-014597-66vd2mdu	1104	16	a	a	DT
cord-014597-66vd2mdu	1104	17	value	value	NN
cord-014597-66vd2mdu	1104	18	-	-	HYPH
cord-014597-66vd2mdu	1104	19	based	base	VBN
cord-014597-66vd2mdu	1104	20	mapping	mapping	NN
cord-014597-66vd2mdu	1104	21	independent	independent	JJ
cord-014597-66vd2mdu	1104	22	of	of	IN
cord-014597-66vd2mdu	1104	23	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1104	24	scale	scale	NN
cord-014597-66vd2mdu	1104	25	.	.	.
cord-014597-66vd2mdu	1105	1	Process	process	VB
cord-014597-66vd2mdu	1105	2	insight	insight	JJ
cord-014597-66vd2mdu	1105	3	information	information	NN
cord-014597-66vd2mdu	1105	4	(	(	-LRB-
cord-014597-66vd2mdu	1105	5	established	establish	VBN
cord-014597-66vd2mdu	1105	6	either	either	CC
cord-014597-66vd2mdu	1105	7	from	from	IN
cord-014597-66vd2mdu	1105	8	DoE	DoE	NNP
cord-014597-66vd2mdu	1105	9	process	process	NNP
cord-014597-66vd2mdu	1105	10	investigation	investigation	NN
cord-014597-66vd2mdu	1105	11	or	or	CC
cord-014597-66vd2mdu	1105	12	existing	existing	JJ
cord-014597-66vd2mdu	1105	13	experience	experience	NN
cord-014597-66vd2mdu	1105	14	with	with	IN
cord-014597-66vd2mdu	1105	15	a	a	DT
cord-014597-66vd2mdu	1105	16	process	process	NN
cord-014597-66vd2mdu	1105	17	platform	platform	NN
cord-014597-66vd2mdu	1105	18	)	)	-RRB-
cord-014597-66vd2mdu	1105	19	is	be	VBZ
cord-014597-66vd2mdu	1105	20	directly	directly	RB
cord-014597-66vd2mdu	1105	21	formalized	formalize	VBN
cord-014597-66vd2mdu	1105	22	into	into	IN
cord-014597-66vd2mdu	1105	23	a	a	DT
cord-014597-66vd2mdu	1105	24	set	set	NN
cord-014597-66vd2mdu	1105	25	of	of	IN
cord-014597-66vd2mdu	1105	26	mappings	mapping	NNS
cord-014597-66vd2mdu	1105	27	which	which	WDT
cord-014597-66vd2mdu	1105	28	transform	transform	VBP
cord-014597-66vd2mdu	1105	29	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	1105	30	values	value	NNS
cord-014597-66vd2mdu	1105	31	into	into	IN
cord-014597-66vd2mdu	1105	32	perceived	perceive	VBN
cord-014597-66vd2mdu	1105	33	benefits	benefit	NNS
cord-014597-66vd2mdu	1105	34	(	(	-LRB-
cord-014597-66vd2mdu	1105	35	0	0	CD
cord-014597-66vd2mdu	1105	36	to	to	IN
cord-014597-66vd2mdu	1105	37	1	1	CD
cord-014597-66vd2mdu	1105	38	)	)	-RRB-
cord-014597-66vd2mdu	1105	39	.	.	.
cord-014597-66vd2mdu	1106	1	At	at	IN
cord-014597-66vd2mdu	1106	2	each	each	DT
cord-014597-66vd2mdu	1106	3	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1106	4	scale	scale	NN
cord-014597-66vd2mdu	1106	5	,	,	,
cord-014597-66vd2mdu	1106	6	parameters	parameter	NNS
cord-014597-66vd2mdu	1106	7	(	(	-LRB-
cord-014597-66vd2mdu	1106	8	e.g.	e.g.	RB
cord-014597-66vd2mdu	1106	9	stirring	stir	VBG
cord-014597-66vd2mdu	1106	10	and	and	CC
cord-014597-66vd2mdu	1106	11	gassing	gassing	NN
cord-014597-66vd2mdu	1106	12	)	)	-RRB-
cord-014597-66vd2mdu	1106	13	are	be	VBP
cord-014597-66vd2mdu	1106	14	then	then	RB
cord-014597-66vd2mdu	1106	15	chosen	choose	VBN
cord-014597-66vd2mdu	1106	16	to	to	TO
cord-014597-66vd2mdu	1106	17	maximize	maximize	VB
cord-014597-66vd2mdu	1106	18	the	the	DT
cord-014597-66vd2mdu	1106	19	product	product	NN
cord-014597-66vd2mdu	1106	20	of	of	IN
cord-014597-66vd2mdu	1106	21	resultant	resultant	JJ
cord-014597-66vd2mdu	1106	22	Utility	Utility	NNP
cord-014597-66vd2mdu	1106	23	Functions	function	NNS
cord-014597-66vd2mdu	1106	24	.	.	.
cord-014597-66vd2mdu	1107	1	The	the	DT
cord-014597-66vd2mdu	1107	2	model	model	NN
cord-014597-66vd2mdu	1107	3	CHO	CHO	NNP
cord-014597-66vd2mdu	1107	4	fed	fed	NNP
cord-014597-66vd2mdu	1107	5	-	-	HYPH
cord-014597-66vd2mdu	1107	6	batch	batch	NN
cord-014597-66vd2mdu	1107	7	process	process	NN
cord-014597-66vd2mdu	1107	8	in	in	IN
cord-014597-66vd2mdu	1107	9	this	this	DT
cord-014597-66vd2mdu	1107	10	trial	trial	NN
cord-014597-66vd2mdu	1107	11	comprised	comprise	VBD
cord-014597-66vd2mdu	1107	12	a	a	DT
cord-014597-66vd2mdu	1107	13	CHO	CHO	NNP
cord-014597-66vd2mdu	1107	14	DG44	DG44	NNP
cord-014597-66vd2mdu	1107	15	cell	cell	NN
cord-014597-66vd2mdu	1107	16	line	line	NN
cord-014597-66vd2mdu	1107	17	that	that	WDT
cord-014597-66vd2mdu	1107	18	was	be	VBD
cord-014597-66vd2mdu	1107	19	transfected	transfecte	VBN
cord-014597-66vd2mdu	1107	20	to	to	TO
cord-014597-66vd2mdu	1107	21	produce	produce	VB
cord-014597-66vd2mdu	1107	22	a	a	DT
cord-014597-66vd2mdu	1107	23	humanized	humanize	VBN
cord-014597-66vd2mdu	1107	24	antibody	antibody	NN
cord-014597-66vd2mdu	1107	25	IgG1	igg1	NN
cord-014597-66vd2mdu	1107	26	.	.	.
cord-014597-66vd2mdu	1108	1	A	a	DT
cord-014597-66vd2mdu	1108	2	chemically	chemically	RB
cord-014597-66vd2mdu	1108	3	defined	define	VBN
cord-014597-66vd2mdu	1108	4	media	medium	NNS
cord-014597-66vd2mdu	1108	5	system	system	NN
cord-014597-66vd2mdu	1108	6	was	be	VBD
cord-014597-66vd2mdu	1108	7	used	use	VBN
cord-014597-66vd2mdu	1108	8	.	.	.
cord-014597-66vd2mdu	1109	1	The	the	DT
cord-014597-66vd2mdu	1109	2	process	process	NN
cord-014597-66vd2mdu	1109	3	,	,	,
cord-014597-66vd2mdu	1109	4	including	include	VBG
cord-014597-66vd2mdu	1109	5	cell	cell	NN
cord-014597-66vd2mdu	1109	6	line	line	NN
cord-014597-66vd2mdu	1109	7	,	,	,
cord-014597-66vd2mdu	1109	8	medium	medium	NN
cord-014597-66vd2mdu	1109	9	and	and	CC
cord-014597-66vd2mdu	1109	10	feeding	feeding	NN
cord-014597-66vd2mdu	1109	11	strategy	strategy	NN
cord-014597-66vd2mdu	1109	12	was	be	VBD
cord-014597-66vd2mdu	1109	13	designed	design	VBN
cord-014597-66vd2mdu	1109	14	and	and	CC
cord-014597-66vd2mdu	1109	15	developed	develop	VBN
cord-014597-66vd2mdu	1109	16	by	by	IN
cord-014597-66vd2mdu	1109	17	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	1109	18	Stedim	Stedim	NNP
cord-014597-66vd2mdu	1109	19	Cellca	Cellca	NNP
cord-014597-66vd2mdu	1109	20	.	.	.
cord-014597-66vd2mdu	1110	1	The	the	DT
cord-014597-66vd2mdu	1110	2	aim	aim	NN
cord-014597-66vd2mdu	1110	3	of	of	IN
cord-014597-66vd2mdu	1110	4	the	the	DT
cord-014597-66vd2mdu	1110	5	gassing	gassing	NN
cord-014597-66vd2mdu	1110	6	scale	scale	NN
cord-014597-66vd2mdu	1110	7	-	-	HYPH
cord-014597-66vd2mdu	1110	8	up	up	NN
cord-014597-66vd2mdu	1110	9	was	be	VBD
cord-014597-66vd2mdu	1110	10	to	to	TO
cord-014597-66vd2mdu	1110	11	achieve	achieve	VB
cord-014597-66vd2mdu	1110	12	similar	similar	JJ
cord-014597-66vd2mdu	1110	13	cell	cell	NN
cord-014597-66vd2mdu	1110	14	densities	density	NNS
cord-014597-66vd2mdu	1110	15	when	when	WRB
cord-014597-66vd2mdu	1110	16	the	the	DT
cord-014597-66vd2mdu	1110	17	addition	addition	NN
cord-014597-66vd2mdu	1110	18	of	of	IN
cord-014597-66vd2mdu	1110	19	pure	pure	JJ
cord-014597-66vd2mdu	1110	20	oxygen	oxygen	NN
cord-014597-66vd2mdu	1110	21	starts	start	NNS
cord-014597-66vd2mdu	1110	22	.	.	.
cord-014597-66vd2mdu	1111	1	For	for	IN
cord-014597-66vd2mdu	1111	2	all	all	DT
cord-014597-66vd2mdu	1111	3	Flexsafe	Flexsafe	NNP
cord-014597-66vd2mdu	1111	4	STR	STR	NNP
cord-014597-66vd2mdu	1111	5	®	®	NN
cord-014597-66vd2mdu	1111	6	bags	bag	NNS
cord-014597-66vd2mdu	1111	7	oxygen	oxygen	NN
cord-014597-66vd2mdu	1111	8	was	be	VBD
cord-014597-66vd2mdu	1111	9	sparged	sparge	VBN
cord-014597-66vd2mdu	1111	10	via	via	IN
cord-014597-66vd2mdu	1111	11	the	the	DT
cord-014597-66vd2mdu	1111	12	micro	micro	NNP
cord-014597-66vd2mdu	1111	13	sparger	sparger	NNP
cord-014597-66vd2mdu	1111	14	part	part	NN
cord-014597-66vd2mdu	1111	15	of	of	IN
cord-014597-66vd2mdu	1111	16	the	the	DT
cord-014597-66vd2mdu	1111	17	combi	combi	NN
cord-014597-66vd2mdu	1111	18	sparger	sparger	NN
cord-014597-66vd2mdu	1111	19	.	.	.
cord-014597-66vd2mdu	1112	1	All	all	DT
cord-014597-66vd2mdu	1112	2	other	other	JJ
cord-014597-66vd2mdu	1112	3	systems	system	NNS
cord-014597-66vd2mdu	1112	4	used	use	VBD
cord-014597-66vd2mdu	1112	5	a	a	DT
cord-014597-66vd2mdu	1112	6	ring	ring	NN
cord-014597-66vd2mdu	1112	7	sparger	sparger	NN
cord-014597-66vd2mdu	1112	8	with	with	IN
cord-014597-66vd2mdu	1112	9	holes	hole	NNS
cord-014597-66vd2mdu	1112	10	face	face	VBP
cord-014597-66vd2mdu	1112	11	up	up	RP
cord-014597-66vd2mdu	1112	12	.	.	.
cord-014597-66vd2mdu	1113	1	The	the	DT
cord-014597-66vd2mdu	1113	2	initial	initial	JJ
cord-014597-66vd2mdu	1113	3	air	air	NN
cord-014597-66vd2mdu	1113	4	flow	flow	NN
cord-014597-66vd2mdu	1113	5	rate	rate	NN
cord-014597-66vd2mdu	1113	6	was	be	VBD
cord-014597-66vd2mdu	1113	7	set	set	VBN
cord-014597-66vd2mdu	1113	8	to	to	IN
cord-014597-66vd2mdu	1113	9	an	an	DT
cord-014597-66vd2mdu	1113	10	oxygen	oxygen	NN
cord-014597-66vd2mdu	1113	11	transfer	transfer	NN
cord-014597-66vd2mdu	1113	12	rate	rate	NN
cord-014597-66vd2mdu	1113	13	(	(	-LRB-
cord-014597-66vd2mdu	1113	14	k	k	NNP
cord-014597-66vd2mdu	1113	15	L	L	NNP
cord-014597-66vd2mdu	1113	16	a	a	NNP
cord-014597-66vd2mdu	1113	17	)	)	-RRB-
cord-014597-66vd2mdu	1113	18	of	of	IN
cord-014597-66vd2mdu	1113	19	8	8	CD
cord-014597-66vd2mdu	1113	20	1	1	CD
cord-014597-66vd2mdu	1113	21	/	/	SYM
cord-014597-66vd2mdu	1113	22	h	h	NN
cord-014597-66vd2mdu	1113	23	at	at	IN
cord-014597-66vd2mdu	1113	24	the	the	DT
cord-014597-66vd2mdu	1113	25	corresponding	corresponding	JJ
cord-014597-66vd2mdu	1113	26	agitation	agitation	NN
cord-014597-66vd2mdu	1113	27	rate	rate	NN
cord-014597-66vd2mdu	1113	28	and	and	CC
cord-014597-66vd2mdu	1113	29	volume	volume	NN
cord-014597-66vd2mdu	1113	30	.	.	.
cord-014597-66vd2mdu	1114	1	All	all	DT
cord-014597-66vd2mdu	1114	2	process	process	NN
cord-014597-66vd2mdu	1114	3	engineering	engineering	NN
cord-014597-66vd2mdu	1114	4	characterization	characterization	NN
cord-014597-66vd2mdu	1114	5	parameters	parameter	NNS
cord-014597-66vd2mdu	1114	6	were	be	VBD
cord-014597-66vd2mdu	1114	7	determined	determine	VBN
cord-014597-66vd2mdu	1114	8	according	accord	VBG
cord-014597-66vd2mdu	1114	9	to	to	IN
cord-014597-66vd2mdu	1114	10	DECHEMA	DECHEMA	NNP
cord-014597-66vd2mdu	1114	11	guidelines	guideline	NNS
cord-014597-66vd2mdu	1114	12	[	[	-LRB-
cord-014597-66vd2mdu	1114	13	2	2	CD
cord-014597-66vd2mdu	1114	14	]	]	-RRB-
cord-014597-66vd2mdu	1114	15	.	.	.
cord-014597-66vd2mdu	1115	1	With	with	IN
cord-014597-66vd2mdu	1115	2	the	the	DT
cord-014597-66vd2mdu	1115	3	use	use	NN
cord-014597-66vd2mdu	1115	4	of	of	IN
cord-014597-66vd2mdu	1115	5	the	the	DT
cord-014597-66vd2mdu	1115	6	Utility	Utility	NNP
cord-014597-66vd2mdu	1115	7	Functions	function	NNS
cord-014597-66vd2mdu	1115	8	the	the	DT
cord-014597-66vd2mdu	1115	9	discrete	discrete	JJ
cord-014597-66vd2mdu	1115	10	agitation	agitation	NN
cord-014597-66vd2mdu	1115	11	rate	rate	NN
cord-014597-66vd2mdu	1115	12	was	be	VBD
cord-014597-66vd2mdu	1115	13	determined	determine	VBN
cord-014597-66vd2mdu	1115	14	(	(	-LRB-
cord-014597-66vd2mdu	1115	15	Table	table	NN
cord-014597-66vd2mdu	1115	16	1	1	CD
cord-014597-66vd2mdu	1115	17	)	)	-RRB-
cord-014597-66vd2mdu	1115	18	.	.	.
cord-014597-66vd2mdu	1116	1	The	the	DT
cord-014597-66vd2mdu	1116	2	Utility	Utility	NNP
cord-014597-66vd2mdu	1116	3	Functions	Functions	NNPS
cord-014597-66vd2mdu	1116	4	led	lead	VBD
cord-014597-66vd2mdu	1116	5	to	to	IN
cord-014597-66vd2mdu	1116	6	discrete	discrete	JJ
cord-014597-66vd2mdu	1116	7	agitation	agitation	NN
cord-014597-66vd2mdu	1116	8	rates	rate	NNS
cord-014597-66vd2mdu	1116	9	where	where	WRB
cord-014597-66vd2mdu	1116	10	not	not	RB
cord-014597-66vd2mdu	1116	11	only	only	RB
cord-014597-66vd2mdu	1116	12	homogeneous	homogeneous	JJ
cord-014597-66vd2mdu	1116	13	mixing	mixing	NN
cord-014597-66vd2mdu	1116	14	but	but	CC
cord-014597-66vd2mdu	1116	15	also	also	RB
cord-014597-66vd2mdu	1116	16	a	a	DT
cord-014597-66vd2mdu	1116	17	turbulent	turbulent	JJ
cord-014597-66vd2mdu	1116	18	flow	flow	NN
cord-014597-66vd2mdu	1116	19	pattern	pattern	NN
cord-014597-66vd2mdu	1116	20	and	and	CC
cord-014597-66vd2mdu	1116	21	a	a	DT
cord-014597-66vd2mdu	1116	22	suitable	suitable	JJ
cord-014597-66vd2mdu	1116	23	specific	specific	JJ
cord-014597-66vd2mdu	1116	24	power	power	NN
cord-014597-66vd2mdu	1116	25	input	input	NN
cord-014597-66vd2mdu	1116	26	was	be	VBD
cord-014597-66vd2mdu	1116	27	guaranteed	guarantee	VBN
cord-014597-66vd2mdu	1116	28	.	.	.
cord-014597-66vd2mdu	1117	1	The	the	DT
cord-014597-66vd2mdu	1117	2	initial	initial	JJ
cord-014597-66vd2mdu	1117	3	gassing	gassing	NN
cord-014597-66vd2mdu	1117	4	rate	rate	NN
cord-014597-66vd2mdu	1117	5	of	of	IN
cord-014597-66vd2mdu	1117	6	air	air	NN
cord-014597-66vd2mdu	1117	7	supplied	supply	VBN
cord-014597-66vd2mdu	1117	8	enough	enough	JJ
cord-014597-66vd2mdu	1117	9	oxygen	oxygen	NN
cord-014597-66vd2mdu	1117	10	for	for	IN
cord-014597-66vd2mdu	1117	11	5	5	CD
cord-014597-66vd2mdu	1117	12	x	x	SYM
cord-014597-66vd2mdu	1117	13	10	10	CD
cord-014597-66vd2mdu	1117	14	6	6	CD
cord-014597-66vd2mdu	1117	15	cells	cell	NNS
cord-014597-66vd2mdu	1117	16	/	/	SYM
cord-014597-66vd2mdu	1117	17	mL	ml	NN
cord-014597-66vd2mdu	1117	18	in	in	IN
cord-014597-66vd2mdu	1117	19	all	all	DT
cord-014597-66vd2mdu	1117	20	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	1117	21	.	.	.
cord-014597-66vd2mdu	1118	1	Due	due	IN
cord-014597-66vd2mdu	1118	2	to	to	IN
cord-014597-66vd2mdu	1118	3	the	the	DT
cord-014597-66vd2mdu	1118	4	used	use	VBN
cord-014597-66vd2mdu	1118	5	scaling	scaling	NN
cord-014597-66vd2mdu	1118	6	methods	method	NNS
cord-014597-66vd2mdu	1118	7	the	the	DT
cord-014597-66vd2mdu	1118	8	growth	growth	NN
cord-014597-66vd2mdu	1118	9	patterns	pattern	NNS
cord-014597-66vd2mdu	1118	10	in	in	IN
cord-014597-66vd2mdu	1118	11	all	all	DT
cord-014597-66vd2mdu	1118	12	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1118	13	scales	scale	NNS
cord-014597-66vd2mdu	1118	14	were	be	VBD
cord-014597-66vd2mdu	1118	15	comparable	comparable	JJ
cord-014597-66vd2mdu	1118	16	.	.	.
cord-014597-66vd2mdu	1119	1	Peak	peak	JJ
cord-014597-66vd2mdu	1119	2	viable	viable	JJ
cord-014597-66vd2mdu	1119	3	cell	cell	NN
cord-014597-66vd2mdu	1119	4	densities	density	NNS
cord-014597-66vd2mdu	1119	5	(	(	-LRB-
cord-014597-66vd2mdu	1119	6	VCD	VCD	NNP
cord-014597-66vd2mdu	1119	7	)	)	-RRB-
cord-014597-66vd2mdu	1119	8	of	of	IN
cord-014597-66vd2mdu	1119	9	20	20	CD
cord-014597-66vd2mdu	1119	10	-26	-26	CD
cord-014597-66vd2mdu	1119	11	x	x	SYM
cord-014597-66vd2mdu	1119	12	10	10	CD
cord-014597-66vd2mdu	1119	13	6	6	CD
cord-014597-66vd2mdu	1119	14	cells	cell	NNS
cord-014597-66vd2mdu	1119	15	/	/	SYM
cord-014597-66vd2mdu	1119	16	mL	mL	NNS
cord-014597-66vd2mdu	1119	17	were	be	VBD
cord-014597-66vd2mdu	1119	18	achieved	achieve	VBN
cord-014597-66vd2mdu	1119	19	and	and	CC
cord-014597-66vd2mdu	1119	20	viability	viability	NN
cord-014597-66vd2mdu	1119	21	at	at	IN
cord-014597-66vd2mdu	1119	22	the	the	DT
cord-014597-66vd2mdu	1119	23	point	point	NN
cord-014597-66vd2mdu	1119	24	of	of	IN
cord-014597-66vd2mdu	1119	25	harvest	harvest	NN
cord-014597-66vd2mdu	1119	26	was	be	VBD
cord-014597-66vd2mdu	1119	27	above	above	IN
cord-014597-66vd2mdu	1119	28	80	80	CD
cord-014597-66vd2mdu	1119	29	%	%	NN
cord-014597-66vd2mdu	1119	30	in	in	IN
cord-014597-66vd2mdu	1119	31	all	all	DT
cord-014597-66vd2mdu	1119	32	scales	scale	NNS
cord-014597-66vd2mdu	1119	33	.	.	.
cord-014597-66vd2mdu	1120	1	The	the	DT
cord-014597-66vd2mdu	1120	2	final	final	JJ
cord-014597-66vd2mdu	1120	3	product	product	NN
cord-014597-66vd2mdu	1120	4	concentration	concentration	NN
cord-014597-66vd2mdu	1120	5	was	be	VBD
cord-014597-66vd2mdu	1120	6	in	in	IN
cord-014597-66vd2mdu	1120	7	an	an	DT
cord-014597-66vd2mdu	1120	8	acceptable	acceptable	JJ
cord-014597-66vd2mdu	1120	9	range	range	NN
cord-014597-66vd2mdu	1120	10	of	of	IN
cord-014597-66vd2mdu	1120	11	2.9	2.9	CD
cord-014597-66vd2mdu	1120	12	-3.6	-3.6	NNP
cord-014597-66vd2mdu	1120	13	g	g	NNP
cord-014597-66vd2mdu	1120	14	/	/	SYM
cord-014597-66vd2mdu	1120	15	L.	L.	NNP
cord-014597-66vd2mdu	1120	16	Product	Product	NNP
cord-014597-66vd2mdu	1120	17	quality	quality	NN
cord-014597-66vd2mdu	1120	18	attributes	attribute	VBZ
cord-014597-66vd2mdu	1120	19	show	show	VBP
cord-014597-66vd2mdu	1120	20	comparability	comparability	NN
cord-014597-66vd2mdu	1120	21	over	over	IN
cord-014597-66vd2mdu	1120	22	the	the	DT
cord-014597-66vd2mdu	1120	23	complete	complete	JJ
cord-014597-66vd2mdu	1120	24	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1120	25	range	range	NN
cord-014597-66vd2mdu	1120	26	(	(	-LRB-
cord-014597-66vd2mdu	1120	27	Fig	fig	NN
cord-014597-66vd2mdu	1120	28	.	.	.
cord-014597-66vd2mdu	1120	29	1	1	CD
cord-014597-66vd2mdu	1120	30	)	)	-RRB-
cord-014597-66vd2mdu	1120	31	.	.	.
cord-014597-66vd2mdu	1121	1	The	the	DT
cord-014597-66vd2mdu	1121	2	harvest	harvest	NN
cord-014597-66vd2mdu	1121	3	criteria	criterion	NNS
cord-014597-66vd2mdu	1121	4	of	of	IN
cord-014597-66vd2mdu	1121	5	12	12	CD
cord-014597-66vd2mdu	1121	6	days	day	NNS
cord-014597-66vd2mdu	1121	7	gave	give	VBD
cord-014597-66vd2mdu	1121	8	a	a	DT
cord-014597-66vd2mdu	1121	9	combination	combination	NN
cord-014597-66vd2mdu	1121	10	of	of	IN
cord-014597-66vd2mdu	1121	11	viability	viability	NN
cord-014597-66vd2mdu	1121	12	and	and	CC
cord-014597-66vd2mdu	1121	13	product	product	NN
cord-014597-66vd2mdu	1121	14	concentration	concentration	NN
cord-014597-66vd2mdu	1121	15	that	that	WDT
cord-014597-66vd2mdu	1121	16	made	make	VBD
cord-014597-66vd2mdu	1121	17	it	-PRON-	PRP
cord-014597-66vd2mdu	1121	18	easy	easy	JJ
cord-014597-66vd2mdu	1121	19	to	to	TO
cord-014597-66vd2mdu	1121	20	process	process	VB
cord-014597-66vd2mdu	1121	21	the	the	DT
cord-014597-66vd2mdu	1121	22	cell	cell	NN
cord-014597-66vd2mdu	1121	23	broth	broth	NN
cord-014597-66vd2mdu	1121	24	during	during	IN
cord-014597-66vd2mdu	1121	25	cell	cell	NN
cord-014597-66vd2mdu	1121	26	removal	removal	NN
cord-014597-66vd2mdu	1121	27	and	and	CC
cord-014597-66vd2mdu	1121	28	other	other	JJ
cord-014597-66vd2mdu	1121	29	downstream	downstream	JJ
cord-014597-66vd2mdu	1121	30	steps	step	NNS
cord-014597-66vd2mdu	1121	31	.	.	.
cord-014597-66vd2mdu	1122	1	The	the	DT
cord-014597-66vd2mdu	1122	2	process	process	NN
cord-014597-66vd2mdu	1122	3	implementation	implementation	NN
cord-014597-66vd2mdu	1122	4	of	of	IN
cord-014597-66vd2mdu	1122	5	the	the	DT
cord-014597-66vd2mdu	1122	6	CHO	CHO	NNP
cord-014597-66vd2mdu	1122	7	production	production	NN
cord-014597-66vd2mdu	1122	8	System	system	NN
cord-014597-66vd2mdu	1122	9	-Expressing	-Expressing	NNP
cord-014597-66vd2mdu	1122	10	mAb	mab	NN
cord-014597-66vd2mdu	1122	11	2	2	CD
cord-014597-66vd2mdu	1122	12	was	be	VBD
cord-014597-66vd2mdu	1122	13	successfully	successfully	RB
cord-014597-66vd2mdu	1122	14	performed	perform	VBN
cord-014597-66vd2mdu	1122	15	with	with	IN
cord-014597-66vd2mdu	1122	16	the	the	DT
cord-014597-66vd2mdu	1122	17	use	use	NN
cord-014597-66vd2mdu	1122	18	of	of	IN
cord-014597-66vd2mdu	1122	19	Utility	Utility	NNP
cord-014597-66vd2mdu	1122	20	Functions	Functions	NNPS
cord-014597-66vd2mdu	1122	21	.	.	.
cord-014597-66vd2mdu	1123	1	Cell	cell	NN
cord-014597-66vd2mdu	1123	2	growth	growth	NN
cord-014597-66vd2mdu	1123	3	,	,	,
cord-014597-66vd2mdu	1123	4	productivity	productivity	NN
cord-014597-66vd2mdu	1123	5	and	and	CC
cord-014597-66vd2mdu	1123	6	product	product	NN
cord-014597-66vd2mdu	1123	7	quality	quality	NN
cord-014597-66vd2mdu	1123	8	is	be	VBZ
cord-014597-66vd2mdu	1123	9	comparable	comparable	JJ
cord-014597-66vd2mdu	1123	10	over	over	IN
cord-014597-66vd2mdu	1123	11	the	the	DT
cord-014597-66vd2mdu	1123	12	complete	complete	JJ
cord-014597-66vd2mdu	1123	13	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1123	14	range	range	NN
cord-014597-66vd2mdu	1123	15	.	.	.
cord-014597-66vd2mdu	1124	1	Background	Background	NNP
cord-014597-66vd2mdu	1124	2	Endoplasmic	Endoplasmic	NNP
cord-014597-66vd2mdu	1124	3	reticulum	reticulum	NN
cord-014597-66vd2mdu	1124	4	(	(	-LRB-
cord-014597-66vd2mdu	1124	5	ER	ER	NNP
cord-014597-66vd2mdu	1124	6	)	)	-RRB-
cord-014597-66vd2mdu	1124	7	,	,	,
cord-014597-66vd2mdu	1124	8	the	the	DT
cord-014597-66vd2mdu	1124	9	central	central	JJ
cord-014597-66vd2mdu	1124	10	part	part	NN
cord-014597-66vd2mdu	1124	11	of	of	IN
cord-014597-66vd2mdu	1124	12	the	the	DT
cord-014597-66vd2mdu	1124	13	secretory	secretory	JJ
cord-014597-66vd2mdu	1124	14	pathways	pathway	NNS
cord-014597-66vd2mdu	1124	15	in	in	IN
cord-014597-66vd2mdu	1124	16	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	1124	17	cells	cell	NNS
cord-014597-66vd2mdu	1124	18	,	,	,
cord-014597-66vd2mdu	1124	19	is	be	VBZ
cord-014597-66vd2mdu	1124	20	responsible	responsible	JJ
cord-014597-66vd2mdu	1124	21	for	for	IN
cord-014597-66vd2mdu	1124	22	controlling	control	VBG
cord-014597-66vd2mdu	1124	23	the	the	DT
cord-014597-66vd2mdu	1124	24	quality	quality	NN
cord-014597-66vd2mdu	1124	25	of	of	IN
cord-014597-66vd2mdu	1124	26	secreted	secrete	VBN
cord-014597-66vd2mdu	1124	27	and	and	CC
cord-014597-66vd2mdu	1124	28	resident	resident	NN
cord-014597-66vd2mdu	1124	29	proteins	protein	NNS
cord-014597-66vd2mdu	1124	30	through	through	IN
cord-014597-66vd2mdu	1124	31	the	the	DT
cord-014597-66vd2mdu	1124	32	regulation	regulation	NN
cord-014597-66vd2mdu	1124	33	of	of	IN
cord-014597-66vd2mdu	1124	34	protein	protein	NN
cord-014597-66vd2mdu	1124	35	translocation	translocation	NN
cord-014597-66vd2mdu	1124	36	,	,	,
cord-014597-66vd2mdu	1124	37	protein	protein	NN
cord-014597-66vd2mdu	1124	38	folding	folding	NN
cord-014597-66vd2mdu	1124	39	,	,	,
cord-014597-66vd2mdu	1124	40	and	and	CC
cord-014597-66vd2mdu	1124	41	early	early	JJ
cord-014597-66vd2mdu	1124	42	post	post	JJ
cord-014597-66vd2mdu	1124	43	-	-	JJ
cord-014597-66vd2mdu	1124	44	translational	translational	JJ
cord-014597-66vd2mdu	1124	45	modifications	modification	NNS
cord-014597-66vd2mdu	1124	46	[	[	-LRB-
cord-014597-66vd2mdu	1124	47	1	1	CD
cord-014597-66vd2mdu	1124	48	]	]	-RRB-
cord-014597-66vd2mdu	1124	49	.	.	.
cord-014597-66vd2mdu	1125	1	A	a	DT
cord-014597-66vd2mdu	1125	2	number	number	NN
cord-014597-66vd2mdu	1125	3	of	of	IN
cord-014597-66vd2mdu	1125	4	physiological	physiological	JJ
cord-014597-66vd2mdu	1125	5	conditions	condition	NNS
cord-014597-66vd2mdu	1125	6	such	such	JJ
cord-014597-66vd2mdu	1125	7	as	as	IN
cord-014597-66vd2mdu	1125	8	oxidative	oxidative	JJ
cord-014597-66vd2mdu	1125	9	stress	stress	NN
cord-014597-66vd2mdu	1125	10	,	,	,
cord-014597-66vd2mdu	1125	11	hypoglycemia	hypoglycemia	NN
cord-014597-66vd2mdu	1125	12	,	,	,
cord-014597-66vd2mdu	1125	13	acidosis	acidosis	NN
cord-014597-66vd2mdu	1125	14	,	,	,
cord-014597-66vd2mdu	1125	15	and	and	CC
cord-014597-66vd2mdu	1125	16	thermal	thermal	JJ
cord-014597-66vd2mdu	1125	17	instability	instability	NN
cord-014597-66vd2mdu	1125	18	can	can	MD
cord-014597-66vd2mdu	1125	19	disturb	disturb	VB
cord-014597-66vd2mdu	1125	20	the	the	DT
cord-014597-66vd2mdu	1125	21	ER	ER	NNP
cord-014597-66vd2mdu	1125	22	functions	function	NNS
cord-014597-66vd2mdu	1125	23	,	,	,
cord-014597-66vd2mdu	1125	24	which	which	WDT
cord-014597-66vd2mdu	1125	25	triggers	trigger	VBZ
cord-014597-66vd2mdu	1125	26	ER	ER	NNP
cord-014597-66vd2mdu	1125	27	stress	stress	NN
cord-014597-66vd2mdu	1125	28	[	[	-LRB-
cord-014597-66vd2mdu	1125	29	2	2	CD
cord-014597-66vd2mdu	1125	30	]	]	-RRB-
cord-014597-66vd2mdu	1125	31	.	.	.
cord-014597-66vd2mdu	1126	1	Prolonged	prolonged	JJ
cord-014597-66vd2mdu	1126	2	ER	ER	NNP
cord-014597-66vd2mdu	1126	3	stress	stress	NN
cord-014597-66vd2mdu	1126	4	induces	induce	VBZ
cord-014597-66vd2mdu	1126	5	apoptotic	apoptotic	JJ
cord-014597-66vd2mdu	1126	6	cell	cell	NN
cord-014597-66vd2mdu	1126	7	death	death	NN
cord-014597-66vd2mdu	1126	8	[	[	-LRB-
cord-014597-66vd2mdu	1126	9	3	3	CD
cord-014597-66vd2mdu	1126	10	]	]	-RRB-
cord-014597-66vd2mdu	1126	11	.	.	.
cord-014597-66vd2mdu	1127	1	Oxidative	oxidative	JJ
cord-014597-66vd2mdu	1127	2	stress	stress	NN
cord-014597-66vd2mdu	1127	3	that	that	WDT
cord-014597-66vd2mdu	1127	4	naturally	naturally	RB
cord-014597-66vd2mdu	1127	5	accumulates	accumulate	VBZ
cord-014597-66vd2mdu	1127	6	in	in	IN
cord-014597-66vd2mdu	1127	7	the	the	DT
cord-014597-66vd2mdu	1127	8	ER	ER	NNP
cord-014597-66vd2mdu	1127	9	as	as	IN
cord-014597-66vd2mdu	1127	10	a	a	DT
cord-014597-66vd2mdu	1127	11	result	result	NN
cord-014597-66vd2mdu	1127	12	of	of	IN
cord-014597-66vd2mdu	1127	13	mitochondrial	mitochondrial	JJ
cord-014597-66vd2mdu	1127	14	energy	energy	NN
cord-014597-66vd2mdu	1127	15	metabolism	metabolism	NN
cord-014597-66vd2mdu	1127	16	and	and	CC
cord-014597-66vd2mdu	1127	17	protein	protein	NN
cord-014597-66vd2mdu	1127	18	synthesis	synthesis	NN
cord-014597-66vd2mdu	1127	19	can	can	MD
cord-014597-66vd2mdu	1127	20	disturb	disturb	VB
cord-014597-66vd2mdu	1127	21	the	the	DT
cord-014597-66vd2mdu	1127	22	ER	ER	NNP
cord-014597-66vd2mdu	1127	23	function	function	NN
cord-014597-66vd2mdu	1127	24	[	[	-LRB-
cord-014597-66vd2mdu	1127	25	4	4	CD
cord-014597-66vd2mdu	1127	26	]	]	-RRB-
cord-014597-66vd2mdu	1127	27	.	.	.
cord-014597-66vd2mdu	1128	1	Because	because	IN
cord-014597-66vd2mdu	1128	2	ER	ER	NNP
cord-014597-66vd2mdu	1128	3	has	have	VBZ
cord-014597-66vd2mdu	1128	4	a	a	DT
cord-014597-66vd2mdu	1128	5	responsibility	responsibility	NN
cord-014597-66vd2mdu	1128	6	on	on	IN
cord-014597-66vd2mdu	1128	7	the	the	DT
cord-014597-66vd2mdu	1128	8	protein	protein	NN
cord-014597-66vd2mdu	1128	9	synthesis	synthesis	NN
cord-014597-66vd2mdu	1128	10	and	and	CC
cord-014597-66vd2mdu	1128	11	quality	quality	NN
cord-014597-66vd2mdu	1128	12	control	control	NN
cord-014597-66vd2mdu	1128	13	of	of	IN
cord-014597-66vd2mdu	1128	14	the	the	DT
cord-014597-66vd2mdu	1128	15	secreted	secrete	VBN
cord-014597-66vd2mdu	1128	16	proteins	protein	NNS
cord-014597-66vd2mdu	1128	17	,	,	,
cord-014597-66vd2mdu	1128	18	ER	ER	NNP
cord-014597-66vd2mdu	1128	19	homeostasis	homeostasis	NN
cord-014597-66vd2mdu	1128	20	has	have	VBZ
cord-014597-66vd2mdu	1128	21	to	to	TO
cord-014597-66vd2mdu	1128	22	be	be	VB
cord-014597-66vd2mdu	1128	23	well	well	RB
cord-014597-66vd2mdu	1128	24	maintained	maintain	VBN
cord-014597-66vd2mdu	1128	25	.	.	.
cord-014597-66vd2mdu	1129	1	When	when	WRB
cord-014597-66vd2mdu	1129	2	H	h	NN
cord-014597-66vd2mdu	1129	3	2	2	CD
cord-014597-66vd2mdu	1129	4	O	o	NN
cord-014597-66vd2mdu	1129	5	2	2	CD
cord-014597-66vd2mdu	1129	6	,	,	,
cord-014597-66vd2mdu	1129	7	an	an	DT
cord-014597-66vd2mdu	1129	8	oxidative	oxidative	JJ
cord-014597-66vd2mdu	1129	9	stress	stress	NN
cord-014597-66vd2mdu	1129	10	inducer	inducer	NN
cord-014597-66vd2mdu	1129	11	,	,	,
cord-014597-66vd2mdu	1129	12	was	be	VBD
cord-014597-66vd2mdu	1129	13	added	add	VBN
cord-014597-66vd2mdu	1129	14	to	to	IN
cord-014597-66vd2mdu	1129	15	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1129	16	Chinese	chinese	JJ
cord-014597-66vd2mdu	1129	17	hamster	hamster	NN
cord-014597-66vd2mdu	1129	18	ovary	ovary	NN
cord-014597-66vd2mdu	1129	19	(	(	-LRB-
cord-014597-66vd2mdu	1129	20	rCHO	rcho	NN
cord-014597-66vd2mdu	1129	21	)	)	-RRB-
cord-014597-66vd2mdu	1129	22	cell	cell	NN
cord-014597-66vd2mdu	1129	23	cultures	culture	NNS
cord-014597-66vd2mdu	1129	24	,	,	,
cord-014597-66vd2mdu	1129	25	it	-PRON-	PRP
cord-014597-66vd2mdu	1129	26	reduced	reduce	VBD
cord-014597-66vd2mdu	1129	27	cell	cell	NN
cord-014597-66vd2mdu	1129	28	growth	growth	NN
cord-014597-66vd2mdu	1129	29	,	,	,
cord-014597-66vd2mdu	1129	30	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1129	31	antibody	antibody	NN
cord-014597-66vd2mdu	1129	32	(	(	-LRB-
cord-014597-66vd2mdu	1129	33	mAb	mab	NN
cord-014597-66vd2mdu	1129	34	)	)	-RRB-
cord-014597-66vd2mdu	1129	35	production	production	NN
cord-014597-66vd2mdu	1129	36	,	,	,
cord-014597-66vd2mdu	1129	37	and	and	CC
cord-014597-66vd2mdu	1129	38	galactosylated	galactosylate	VBN
cord-014597-66vd2mdu	1129	39	form	form	NN
cord-014597-66vd2mdu	1129	40	of	of	IN
cord-014597-66vd2mdu	1129	41	mAb	mab	NN
cord-014597-66vd2mdu	1129	42	in	in	IN
cord-014597-66vd2mdu	1129	43	a	a	DT
cord-014597-66vd2mdu	1129	44	dose	dose	NN
cord-014597-66vd2mdu	1129	45	-	-	HYPH
cord-014597-66vd2mdu	1129	46	dependent	dependent	JJ
cord-014597-66vd2mdu	1129	47	manner	manner	NN
cord-014597-66vd2mdu	1129	48	.	.	.
cord-014597-66vd2mdu	1130	1	Antioxidants	antioxidant	NNS
cord-014597-66vd2mdu	1130	2	can	can	MD
cord-014597-66vd2mdu	1130	3	reduce	reduce	VB
cord-014597-66vd2mdu	1130	4	the	the	DT
cord-014597-66vd2mdu	1130	5	oxidative	oxidative	JJ
cord-014597-66vd2mdu	1130	6	stress	stress	NN
cord-014597-66vd2mdu	1130	7	level	level	NN
cord-014597-66vd2mdu	1130	8	and	and	CC
cord-014597-66vd2mdu	1130	9	suppress	suppress	VB
cord-014597-66vd2mdu	1130	10	the	the	DT
cord-014597-66vd2mdu	1130	11	apoptotic	apoptotic	JJ
cord-014597-66vd2mdu	1130	12	cell	cell	NN
cord-014597-66vd2mdu	1130	13	death	death	NN
cord-014597-66vd2mdu	1130	14	by	by	IN
cord-014597-66vd2mdu	1130	15	scavenging	scavenge	VBG
cord-014597-66vd2mdu	1130	16	oxygen	oxygen	NN
cord-014597-66vd2mdu	1130	17	free	free	JJ
cord-014597-66vd2mdu	1130	18	radicals	radical	NNS
cord-014597-66vd2mdu	1130	19	,	,	,
cord-014597-66vd2mdu	1130	20	inhibiting	inhibit	VBG
cord-014597-66vd2mdu	1130	21	chain	chain	NN
cord-014597-66vd2mdu	1130	22	reaction	reaction	NN
cord-014597-66vd2mdu	1130	23	of	of	IN
cord-014597-66vd2mdu	1130	24	oxidation	oxidation	NN
cord-014597-66vd2mdu	1130	25	,	,	,
cord-014597-66vd2mdu	1130	26	and	and	CC
cord-014597-66vd2mdu	1130	27	detoxifying	detoxify	VBG
cord-014597-66vd2mdu	1130	28	peroxide	peroxide	NN
cord-014597-66vd2mdu	1130	29	[	[	-LRB-
cord-014597-66vd2mdu	1130	30	5	5	CD
cord-014597-66vd2mdu	1130	31	]	]	-RRB-
cord-014597-66vd2mdu	1130	32	.	.	.
cord-014597-66vd2mdu	1131	1	However	however	RB
cord-014597-66vd2mdu	1131	2	,	,	,
cord-014597-66vd2mdu	1131	3	despite	despite	IN
cord-014597-66vd2mdu	1131	4	the	the	DT
cord-014597-66vd2mdu	1131	5	importance	importance	NN
cord-014597-66vd2mdu	1131	6	of	of	IN
cord-014597-66vd2mdu	1131	7	mass	mass	NN
cord-014597-66vd2mdu	1131	8	production	production	NN
cord-014597-66vd2mdu	1131	9	of	of	IN
cord-014597-66vd2mdu	1131	10	mAbs	mAbs	NNP
cord-014597-66vd2mdu	1131	11	,	,	,
cord-014597-66vd2mdu	1131	12	studies	study	NNS
cord-014597-66vd2mdu	1131	13	on	on	IN
cord-014597-66vd2mdu	1131	14	the	the	DT
cord-014597-66vd2mdu	1131	15	effect	effect	NN
cord-014597-66vd2mdu	1131	16	of	of	IN
cord-014597-66vd2mdu	1131	17	antioxidants	antioxidant	NNS
cord-014597-66vd2mdu	1131	18	on	on	IN
cord-014597-66vd2mdu	1131	19	the	the	DT
cord-014597-66vd2mdu	1131	20	production	production	NN
cord-014597-66vd2mdu	1131	21	and	and	CC
cord-014597-66vd2mdu	1131	22	quality	quality	NN
cord-014597-66vd2mdu	1131	23	of	of	IN
cord-014597-66vd2mdu	1131	24	mAbs	mabs	NN
cord-014597-66vd2mdu	1131	25	in	in	IN
cord-014597-66vd2mdu	1131	26	rCHO	rcho	JJ
cord-014597-66vd2mdu	1131	27	cell	cell	NN
cord-014597-66vd2mdu	1131	28	cultures	culture	NNS
cord-014597-66vd2mdu	1131	29	have	have	VBP
cord-014597-66vd2mdu	1131	30	not	not	RB
cord-014597-66vd2mdu	1131	31	been	be	VBN
cord-014597-66vd2mdu	1131	32	fully	fully	RB
cord-014597-66vd2mdu	1131	33	substantiated	substantiate	VBN
cord-014597-66vd2mdu	1131	34	.	.	.
cord-014597-66vd2mdu	1132	1	To	to	TO
cord-014597-66vd2mdu	1132	2	find	find	VB
cord-014597-66vd2mdu	1132	3	a	a	DT
cord-014597-66vd2mdu	1132	4	more	more	RBR
cord-014597-66vd2mdu	1132	5	effective	effective	JJ
cord-014597-66vd2mdu	1132	6	antioxidant	antioxidant	NN
cord-014597-66vd2mdu	1132	7	in	in	IN
cord-014597-66vd2mdu	1132	8	rCHO	rcho	JJ
cord-014597-66vd2mdu	1132	9	cell	cell	NN
cord-014597-66vd2mdu	1132	10	cultures	culture	NNS
cord-014597-66vd2mdu	1132	11	,	,	,
cord-014597-66vd2mdu	1132	12	six	six	CD
cord-014597-66vd2mdu	1132	13	different	different	JJ
cord-014597-66vd2mdu	1132	14	antioxidants	antioxidant	NNS
cord-014597-66vd2mdu	1132	15	including	include	VBG
cord-014597-66vd2mdu	1132	16	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1132	17	,	,	,
cord-014597-66vd2mdu	1132	18	which	which	WDT
cord-014597-66vd2mdu	1132	19	have	have	VBP
cord-014597-66vd2mdu	1132	20	used	use	VBN
cord-014597-66vd2mdu	1132	21	widely	widely	RB
cord-014597-66vd2mdu	1132	22	in	in	IN
cord-014597-66vd2mdu	1132	23	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1132	24	cell	cell	NN
cord-014597-66vd2mdu	1132	25	cultures	culture	NNS
cord-014597-66vd2mdu	1132	26	,	,	,
cord-014597-66vd2mdu	1132	27	were	be	VBD
cord-014597-66vd2mdu	1132	28	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1132	29	as	as	IN
cord-014597-66vd2mdu	1132	30	chemical	chemical	JJ
cord-014597-66vd2mdu	1132	31	supplements	supplement	NNS
cord-014597-66vd2mdu	1132	32	with	with	IN
cord-014597-66vd2mdu	1132	33	two	two	CD
cord-014597-66vd2mdu	1132	34	different	different	JJ
cord-014597-66vd2mdu	1132	35	rCHO	rcho	NN
cord-014597-66vd2mdu	1132	36	cell	cell	NN
cord-014597-66vd2mdu	1132	37	lines	line	NNS
cord-014597-66vd2mdu	1132	38	producing	produce	VBG
cord-014597-66vd2mdu	1132	39	the	the	DT
cord-014597-66vd2mdu	1132	40	same	same	JJ
cord-014597-66vd2mdu	1132	41	mAb	mAb	NNS
cord-014597-66vd2mdu	1132	42	in	in	IN
cord-014597-66vd2mdu	1132	43	6-well	6-well	CD
cord-014597-66vd2mdu	1132	44	plates	plate	NNS
cord-014597-66vd2mdu	1132	45	.	.	.
cord-014597-66vd2mdu	1133	1	Then	then	RB
cord-014597-66vd2mdu	1133	2	,	,	,
cord-014597-66vd2mdu	1133	3	batch	batch	NN
cord-014597-66vd2mdu	1133	4	and	and	CC
cord-014597-66vd2mdu	1133	5	fed	fed	JJ
cord-014597-66vd2mdu	1133	6	-	-	HYPH
cord-014597-66vd2mdu	1133	7	batch	batch	NN
cord-014597-66vd2mdu	1133	8	cultures	culture	NNS
cord-014597-66vd2mdu	1133	9	were	be	VBD
cord-014597-66vd2mdu	1133	10	performed	perform	VBN
cord-014597-66vd2mdu	1133	11	in	in	IN
cord-014597-66vd2mdu	1133	12	shake	shake	NN
cord-014597-66vd2mdu	1133	13	flasks	flask	NNS
cord-014597-66vd2mdu	1133	14	with	with	IN
cord-014597-66vd2mdu	1133	15	the	the	DT
cord-014597-66vd2mdu	1133	16	supplementation	supplementation	NN
cord-014597-66vd2mdu	1133	17	of	of	IN
cord-014597-66vd2mdu	1133	18	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1133	19	,	,	,
cord-014597-66vd2mdu	1133	20	which	which	WDT
cord-014597-66vd2mdu	1133	21	showed	show	VBD
cord-014597-66vd2mdu	1133	22	the	the	DT
cord-014597-66vd2mdu	1133	23	best	good	JJS
cord-014597-66vd2mdu	1133	24	effect	effect	NN
cord-014597-66vd2mdu	1133	25	on	on	IN
cord-014597-66vd2mdu	1133	26	culture	culture	NN
cord-014597-66vd2mdu	1133	27	performance	performance	NN
cord-014597-66vd2mdu	1133	28	among	among	IN
cord-014597-66vd2mdu	1133	29	the	the	DT
cord-014597-66vd2mdu	1133	30	6	6	CD
cord-014597-66vd2mdu	1133	31	antioxidants	antioxidant	NNS
cord-014597-66vd2mdu	1133	32	.	.	.
cord-014597-66vd2mdu	1134	1	The	the	DT
cord-014597-66vd2mdu	1134	2	reactive	reactive	JJ
cord-014597-66vd2mdu	1134	3	oxygen	oxygen	NN
cord-014597-66vd2mdu	1134	4	species	specie	NNS
cord-014597-66vd2mdu	1134	5	(	(	-LRB-
cord-014597-66vd2mdu	1134	6	ROS	ROS	NNP
cord-014597-66vd2mdu	1134	7	)	)	-RRB-
cord-014597-66vd2mdu	1134	8	and	and	CC
cord-014597-66vd2mdu	1134	9	ER	ER	NNP
cord-014597-66vd2mdu	1134	10	stress	stress	NN
cord-014597-66vd2mdu	1134	11	levels	level	NNS
cord-014597-66vd2mdu	1134	12	were	be	VBD
cord-014597-66vd2mdu	1134	13	measured	measure	VBN
cord-014597-66vd2mdu	1134	14	to	to	TO
cord-014597-66vd2mdu	1134	15	study	study	VB
cord-014597-66vd2mdu	1134	16	the	the	DT
cord-014597-66vd2mdu	1134	17	effect	effect	NN
cord-014597-66vd2mdu	1134	18	of	of	IN
cord-014597-66vd2mdu	1134	19	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1134	20	on	on	IN
cord-014597-66vd2mdu	1134	21	mAb	mab	NN
cord-014597-66vd2mdu	1134	22	production	production	NN
cord-014597-66vd2mdu	1134	23	and	and	CC
cord-014597-66vd2mdu	1134	24	quality	quality	NN
cord-014597-66vd2mdu	1134	25	.	.	.
cord-014597-66vd2mdu	1135	1	Among	among	IN
cord-014597-66vd2mdu	1135	2	these	these	DT
cord-014597-66vd2mdu	1135	3	antioxidants	antioxidant	NNS
cord-014597-66vd2mdu	1135	4	,	,	,
cord-014597-66vd2mdu	1135	5	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1135	6	showed	show	VBD
cord-014597-66vd2mdu	1135	7	the	the	DT
cord-014597-66vd2mdu	1135	8	best	good	JJS
cord-014597-66vd2mdu	1135	9	mAb	mab	NN
cord-014597-66vd2mdu	1135	10	production	production	NN
cord-014597-66vd2mdu	1135	11	performance	performance	NN
cord-014597-66vd2mdu	1135	12	.	.	.
cord-014597-66vd2mdu	1136	1	Addition	addition	NN
cord-014597-66vd2mdu	1136	2	of	of	IN
cord-014597-66vd2mdu	1136	3	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1136	4	significantly	significantly	RB
cord-014597-66vd2mdu	1136	5	reduced	reduce	VBD
cord-014597-66vd2mdu	1136	6	the	the	DT
cord-014597-66vd2mdu	1136	7	expression	expression	NN
cord-014597-66vd2mdu	1136	8	level	level	NN
cord-014597-66vd2mdu	1136	9	of	of	IN
cord-014597-66vd2mdu	1136	10	BiP	bip	NN
cord-014597-66vd2mdu	1136	11	and	and	CC
cord-014597-66vd2mdu	1136	12	CHOP	chop	VB
cord-014597-66vd2mdu	1136	13	along	along	RP
cord-014597-66vd2mdu	1136	14	with	with	IN
cord-014597-66vd2mdu	1136	15	reduced	reduce	VBN
cord-014597-66vd2mdu	1136	16	ROS	ROS	NNP
cord-014597-66vd2mdu	1136	17	level	level	NN
cord-014597-66vd2mdu	1136	18	,	,	,
cord-014597-66vd2mdu	1136	19	suggesting	suggest	VBG
cord-014597-66vd2mdu	1136	20	oxidative	oxidative	JJ
cord-014597-66vd2mdu	1136	21	stress	stress	NN
cord-014597-66vd2mdu	1136	22	accumulated	accumulate	VBN
cord-014597-66vd2mdu	1136	23	in	in	IN
cord-014597-66vd2mdu	1136	24	the	the	DT
cord-014597-66vd2mdu	1136	25	cells	cell	NNS
cord-014597-66vd2mdu	1136	26	can	can	MD
cord-014597-66vd2mdu	1136	27	be	be	VB
cord-014597-66vd2mdu	1136	28	relieved	relieve	VBN
cord-014597-66vd2mdu	1136	29	using	use	VBG
cord-014597-66vd2mdu	1136	30	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1136	31	.	.	.
cord-014597-66vd2mdu	1137	1	As	as	IN
cord-014597-66vd2mdu	1137	2	a	a	DT
cord-014597-66vd2mdu	1137	3	result	result	NN
cord-014597-66vd2mdu	1137	4	,	,	,
cord-014597-66vd2mdu	1137	5	addition	addition	NN
cord-014597-66vd2mdu	1137	6	of	of	IN
cord-014597-66vd2mdu	1137	7	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1137	8	in	in	IN
cord-014597-66vd2mdu	1137	9	batch	batch	NN
cord-014597-66vd2mdu	1137	10	cultures	culture	NNS
cord-014597-66vd2mdu	1137	11	resulted	result	VBD
cord-014597-66vd2mdu	1137	12	in	in	IN
cord-014597-66vd2mdu	1137	13	1.7	1.7	CD
cord-014597-66vd2mdu	1137	14	-1.8-fold	-1.8-fold	NN
cord-014597-66vd2mdu	1137	15	increase	increase	NN
cord-014597-66vd2mdu	1137	16	in	in	IN
cord-014597-66vd2mdu	1137	17	the	the	DT
cord-014597-66vd2mdu	1137	18	maximum	maximum	JJ
cord-014597-66vd2mdu	1137	19	mAb	mab	NN
cord-014597-66vd2mdu	1137	20	concentration	concentration	NN
cord-014597-66vd2mdu	1137	21	(	(	-LRB-
cord-014597-66vd2mdu	1137	22	MMC	MMC	NNP
cord-014597-66vd2mdu	1137	23	)	)	-RRB-
cord-014597-66vd2mdu	1137	24	,	,	,
cord-014597-66vd2mdu	1137	25	while	while	IN
cord-014597-66vd2mdu	1137	26	maintaining	maintain	VBG
cord-014597-66vd2mdu	1137	27	the	the	DT
cord-014597-66vd2mdu	1137	28	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1137	29	of	of	IN
cord-014597-66vd2mdu	1137	30	mAb	mAb	NNS
cord-014597-66vd2mdu	1137	31	(	(	-LRB-
cord-014597-66vd2mdu	1137	32	Fig	Fig	NNP
cord-014597-66vd2mdu	1137	33	.	.	.
cord-014597-66vd2mdu	1137	34	1	1	CD
cord-014597-66vd2mdu	1137	35	and	and	CC
cord-014597-66vd2mdu	1137	36	Table	Table	NNP
cord-014597-66vd2mdu	1137	37	1	1	CD
cord-014597-66vd2mdu	1137	38	)	)	-RRB-
cord-014597-66vd2mdu	1137	39	.	.	.
cord-014597-66vd2mdu	1138	1	Likewise	likewise	RB
cord-014597-66vd2mdu	1138	2	,	,	,
cord-014597-66vd2mdu	1138	3	addition	addition	NN
cord-014597-66vd2mdu	1138	4	of	of	IN
cord-014597-66vd2mdu	1138	5	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1138	6	in	in	IN
cord-014597-66vd2mdu	1138	7	fed	fed	NNP
cord-014597-66vd2mdu	1138	8	-	-	HYPH
cord-014597-66vd2mdu	1138	9	batch	batch	NN
cord-014597-66vd2mdu	1138	10	culture	culture	NN
cord-014597-66vd2mdu	1138	11	resulted	result	VBD
cord-014597-66vd2mdu	1138	12	in	in	IN
cord-014597-66vd2mdu	1138	13	1.6-fold	1.6-fold	CD
cord-014597-66vd2mdu	1138	14	increase	increase	NN
cord-014597-66vd2mdu	1138	15	in	in	IN
cord-014597-66vd2mdu	1138	16	the	the	DT
cord-014597-66vd2mdu	1138	17	MMC	mmc	NN
cord-014597-66vd2mdu	1138	18	while	while	IN
cord-014597-66vd2mdu	1138	19	maintaining	maintain	VBG
cord-014597-66vd2mdu	1138	20	the	the	DT
cord-014597-66vd2mdu	1138	21	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1138	22	of	of	IN
cord-014597-66vd2mdu	1138	23	mAb	mAb	NNS
cord-014597-66vd2mdu	1138	24	.	.	.
cord-014597-66vd2mdu	1139	1	Oxidative	oxidative	JJ
cord-014597-66vd2mdu	1139	2	stress	stress	NN
cord-014597-66vd2mdu	1139	3	negatively	negatively	RB
cord-014597-66vd2mdu	1139	4	affected	affect	VBD
cord-014597-66vd2mdu	1139	5	the	the	DT
cord-014597-66vd2mdu	1139	6	production	production	NN
cord-014597-66vd2mdu	1139	7	and	and	CC
cord-014597-66vd2mdu	1139	8	galactosylation	galactosylation	NN
cord-014597-66vd2mdu	1139	9	of	of	IN
cord-014597-66vd2mdu	1139	10	mAb	mab	NN
cord-014597-66vd2mdu	1139	11	in	in	IN
cord-014597-66vd2mdu	1139	12	rCHO	rcho	JJ
cord-014597-66vd2mdu	1139	13	cell	cell	NN
cord-014597-66vd2mdu	1139	14	cultures	culture	NNS
cord-014597-66vd2mdu	1139	15	.	.	.
cord-014597-66vd2mdu	1140	1	Among	among	IN
cord-014597-66vd2mdu	1140	2	the	the	DT
cord-014597-66vd2mdu	1140	3	various	various	JJ
cord-014597-66vd2mdu	1140	4	antioxidants	antioxidant	NNS
cord-014597-66vd2mdu	1140	5	tested	test	VBN
cord-014597-66vd2mdu	1140	6	in	in	IN
cord-014597-66vd2mdu	1140	7	this	this	DT
cord-014597-66vd2mdu	1140	8	study	study	NN
cord-014597-66vd2mdu	1140	9	,	,	,
cord-014597-66vd2mdu	1140	10	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1140	11	showed	show	VBD
cord-014597-66vd2mdu	1140	12	the	the	DT
cord-014597-66vd2mdu	1140	13	best	good	JJS
cord-014597-66vd2mdu	1140	14	mAb	mab	NN
cord-014597-66vd2mdu	1140	15	production	production	NN
cord-014597-66vd2mdu	1140	16	performance	performance	NN
cord-014597-66vd2mdu	1140	17	in	in	IN
cord-014597-66vd2mdu	1140	18	both	both	DT
cord-014597-66vd2mdu	1140	19	batch	batch	NN
cord-014597-66vd2mdu	1140	20	and	and	CC
cord-014597-66vd2mdu	1140	21	fed	fed	JJ
cord-014597-66vd2mdu	1140	22	-	-	HYPH
cord-014597-66vd2mdu	1140	23	batch	batch	NN
cord-014597-66vd2mdu	1140	24	cultures	culture	NNS
cord-014597-66vd2mdu	1140	25	of	of	IN
cord-014597-66vd2mdu	1140	26	rCHO	rcho	NN
cord-014597-66vd2mdu	1140	27	cells	cell	NNS
cord-014597-66vd2mdu	1140	28	.	.	.
cord-014597-66vd2mdu	1141	1	Baicalein	Baicalein	NNP
cord-014597-66vd2mdu	1141	2	addition	addition	NN
cord-014597-66vd2mdu	1141	3	significantly	significantly	RB
cord-014597-66vd2mdu	1141	4	enhanced	enhance	VBN
cord-014597-66vd2mdu	1141	5	mAb	mab	NN
cord-014597-66vd2mdu	1141	6	production	production	NN
cord-014597-66vd2mdu	1141	7	while	while	IN
cord-014597-66vd2mdu	1141	8	maintaining	maintain	VBG
cord-014597-66vd2mdu	1141	9	galactosylated	galactosylated	JJ
cord-014597-66vd2mdu	1141	10	forms	form	NNS
cord-014597-66vd2mdu	1141	11	of	of	IN
cord-014597-66vd2mdu	1141	12	mAb	mab	NN
cord-014597-66vd2mdu	1141	13	.	.	.
cord-014597-66vd2mdu	1142	1	Thus	thus	RB
cord-014597-66vd2mdu	1142	2	,	,	,
cord-014597-66vd2mdu	1142	3	baicalein	baicalein	NNP
cord-014597-66vd2mdu	1142	4	is	be	VBZ
cord-014597-66vd2mdu	1142	5	an	an	DT
cord-014597-66vd2mdu	1142	6	effective	effective	JJ
cord-014597-66vd2mdu	1142	7	antioxidant	antioxidant	NN
cord-014597-66vd2mdu	1142	8	for	for	IN
cord-014597-66vd2mdu	1142	9	use	use	NN
cord-014597-66vd2mdu	1142	10	in	in	IN
cord-014597-66vd2mdu	1142	11	rCHO	rcho	JJ
cord-014597-66vd2mdu	1142	12	cell	cell	NN
cord-014597-66vd2mdu	1142	13	cultures	culture	NNS
cord-014597-66vd2mdu	1142	14	for	for	IN
cord-014597-66vd2mdu	1142	15	improved	improve	VBN
cord-014597-66vd2mdu	1142	16	mAb	mab	NN
cord-014597-66vd2mdu	1142	17	production	production	NN
cord-014597-66vd2mdu	1142	18	.	.	.
cord-014597-66vd2mdu	1143	1	Background	background	NN
cord-014597-66vd2mdu	1144	1	The	the	DT
cord-014597-66vd2mdu	1144	2	production	production	NN
cord-014597-66vd2mdu	1144	3	of	of	IN
cord-014597-66vd2mdu	1144	4	many	many	JJ
cord-014597-66vd2mdu	1144	5	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	1144	6	(	(	-LRB-
cord-014597-66vd2mdu	1144	7	e.g.	e.g.	NNP
cord-014597-66vd2mdu	1144	8	antibodies	antibody	NNS
cord-014597-66vd2mdu	1144	9	&	&	CC
cord-014597-66vd2mdu	1144	10	proteins	protein	NNS
cord-014597-66vd2mdu	1144	11	for	for	IN
cord-014597-66vd2mdu	1144	12	diagnostic	diagnostic	JJ
cord-014597-66vd2mdu	1144	13	and	and	CC
cord-014597-66vd2mdu	1144	14	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1144	15	purposes	purpose	NNS
cord-014597-66vd2mdu	1144	16	)	)	-RRB-
cord-014597-66vd2mdu	1144	17	requires	require	VBZ
cord-014597-66vd2mdu	1144	18	the	the	DT
cord-014597-66vd2mdu	1144	19	cultivation	cultivation	NN
cord-014597-66vd2mdu	1144	20	of	of	IN
cord-014597-66vd2mdu	1144	21	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1144	22	cell	cell	NN
cord-014597-66vd2mdu	1144	23	lines	line	NNS
cord-014597-66vd2mdu	1144	24	,	,	,
cord-014597-66vd2mdu	1144	25	which	which	WDT
cord-014597-66vd2mdu	1144	26	is	be	VBZ
cord-014597-66vd2mdu	1144	27	demanding	demand	VBG
cord-014597-66vd2mdu	1144	28	with	with	IN
cord-014597-66vd2mdu	1144	29	respect	respect	NN
cord-014597-66vd2mdu	1144	30	to	to	IN
cord-014597-66vd2mdu	1144	31	various	various	JJ
cord-014597-66vd2mdu	1144	32	aspects	aspect	NNS
cord-014597-66vd2mdu	1144	33	such	such	JJ
cord-014597-66vd2mdu	1144	34	as	as	IN
cord-014597-66vd2mdu	1144	35	complex	complex	JJ
cord-014597-66vd2mdu	1144	36	cell	cell	NN
cord-014597-66vd2mdu	1144	37	metabolism	metabolism	NN
cord-014597-66vd2mdu	1144	38	,	,	,
cord-014597-66vd2mdu	1144	39	variabilities	variability	NNS
cord-014597-66vd2mdu	1144	40	in	in	IN
cord-014597-66vd2mdu	1144	41	cell	cell	NN
cord-014597-66vd2mdu	1144	42	behavior	behavior	NN
cord-014597-66vd2mdu	1144	43	,	,	,
cord-014597-66vd2mdu	1144	44	scale	scale	VB
cord-014597-66vd2mdu	1144	45	dependencies	dependency	NNS
cord-014597-66vd2mdu	1144	46	,	,	,
cord-014597-66vd2mdu	1144	47	influences	influence	NNS
cord-014597-66vd2mdu	1144	48	of	of	IN
cord-014597-66vd2mdu	1144	49	changes	change	NNS
cord-014597-66vd2mdu	1144	50	in	in	IN
cord-014597-66vd2mdu	1144	51	cultivation	cultivation	NN
cord-014597-66vd2mdu	1144	52	conditions	condition	NNS
cord-014597-66vd2mdu	1144	53	,	,	,
cord-014597-66vd2mdu	1144	54	medium	medium	JJ
cord-014597-66vd2mdu	1144	55	composition	composition	NN
cord-014597-66vd2mdu	1144	56	etc	etc	FW
cord-014597-66vd2mdu	1144	57	.	.	.
cord-014597-66vd2mdu	1145	1	Although	although	IN
cord-014597-66vd2mdu	1145	2	an	an	DT
cord-014597-66vd2mdu	1145	3	increasing	increase	VBG
cord-014597-66vd2mdu	1145	4	number	number	NN
cord-014597-66vd2mdu	1145	5	of	of	IN
cord-014597-66vd2mdu	1145	6	measurement	measurement	NN
cord-014597-66vd2mdu	1145	7	parameters	parameter	NNS
cord-014597-66vd2mdu	1145	8	is	be	VBZ
cord-014597-66vd2mdu	1145	9	available	available	JJ
cord-014597-66vd2mdu	1145	10	,	,	,
cord-014597-66vd2mdu	1145	11	only	only	RB
cord-014597-66vd2mdu	1145	12	a	a	DT
cord-014597-66vd2mdu	1145	13	part	part	NN
cord-014597-66vd2mdu	1145	14	of	of	IN
cord-014597-66vd2mdu	1145	15	them	-PRON-	PRP
cord-014597-66vd2mdu	1145	16	is	be	VBZ
cord-014597-66vd2mdu	1145	17	routinely	routinely	RB
cord-014597-66vd2mdu	1145	18	utilized	utilize	VBN
cord-014597-66vd2mdu	1145	19	in	in	IN
cord-014597-66vd2mdu	1145	20	industrial	industrial	JJ
cord-014597-66vd2mdu	1145	21	cell	cell	NN
cord-014597-66vd2mdu	1145	22	culture	culture	NN
cord-014597-66vd2mdu	1145	23	processes	process	NNS
cord-014597-66vd2mdu	1145	24	and	and	CC
cord-014597-66vd2mdu	1145	25	their	-PRON-	PRP$
cord-014597-66vd2mdu	1145	26	corresponding	correspond	VBG
cord-014597-66vd2mdu	1145	27	seed	seed	NN
cord-014597-66vd2mdu	1145	28	trains	train	NNS
cord-014597-66vd2mdu	1145	29	.	.	.
cord-014597-66vd2mdu	1146	1	Nevertheless	nevertheless	RB
cord-014597-66vd2mdu	1146	2	,	,	,
cord-014597-66vd2mdu	1146	3	the	the	DT
cord-014597-66vd2mdu	1146	4	data	data	NN
cord-014597-66vd2mdu	1146	5	base	base	NN
cord-014597-66vd2mdu	1146	6	grows	grow	VBZ
cord-014597-66vd2mdu	1146	7	,	,	,
cord-014597-66vd2mdu	1146	8	statistical	statistical	JJ
cord-014597-66vd2mdu	1146	9	investigation	investigation	NN
cord-014597-66vd2mdu	1146	10	of	of	IN
cord-014597-66vd2mdu	1146	11	data	data	NN
cord-014597-66vd2mdu	1146	12	gains	gain	VBZ
cord-014597-66vd2mdu	1146	13	importance	importance	NN
cord-014597-66vd2mdu	1146	14	and	and	CC
cord-014597-66vd2mdu	1146	15	process	process	NN
cord-014597-66vd2mdu	1146	16	data	datum	NNS
cord-014597-66vd2mdu	1146	17	are	be	VBP
cord-014597-66vd2mdu	1146	18	more	more	RBR
cord-014597-66vd2mdu	1146	19	easily	easily	RB
cord-014597-66vd2mdu	1146	20	accessible	accessible	JJ
cord-014597-66vd2mdu	1146	21	in	in	IN
cord-014597-66vd2mdu	1146	22	the	the	DT
cord-014597-66vd2mdu	1146	23	context	context	NN
cord-014597-66vd2mdu	1146	24	of	of	IN
cord-014597-66vd2mdu	1146	25	industry	industry	NN
cord-014597-66vd2mdu	1146	26	4.0	4.0	CD
cord-014597-66vd2mdu	1146	27	.	.	.
cord-014597-66vd2mdu	1147	1	Cell	cell	NN
cord-014597-66vd2mdu	1147	2	cultivation	cultivation	NN
cord-014597-66vd2mdu	1147	3	has	have	VBZ
cord-014597-66vd2mdu	1147	4	to	to	TO
cord-014597-66vd2mdu	1147	5	consider	consider	VB
cord-014597-66vd2mdu	1147	6	these	these	DT
cord-014597-66vd2mdu	1147	7	complex	complex	JJ
cord-014597-66vd2mdu	1147	8	requirements	requirement	NNS
cord-014597-66vd2mdu	1147	9	,	,	,
cord-014597-66vd2mdu	1147	10	e.g.	e.g.	RB
cord-014597-66vd2mdu	1147	11	for	for	IN
cord-014597-66vd2mdu	1147	12	fed	fed	NN
cord-014597-66vd2mdu	1147	13	-	-	HYPH
cord-014597-66vd2mdu	1147	14	batch	batch	NN
cord-014597-66vd2mdu	1147	15	control	control	NN
cord-014597-66vd2mdu	1147	16	and	and	CC
cord-014597-66vd2mdu	1147	17	seed	seed	NN
cord-014597-66vd2mdu	1147	18	train	train	NN
cord-014597-66vd2mdu	1147	19	design	design	NN
cord-014597-66vd2mdu	1147	20	.	.	.
cord-014597-66vd2mdu	1148	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1148	2	,	,	,
cord-014597-66vd2mdu	1148	3	cultivation	cultivation	NN
cord-014597-66vd2mdu	1148	4	strategies	strategy	NNS
cord-014597-66vd2mdu	1148	5	have	have	VBP
cord-014597-66vd2mdu	1148	6	to	to	TO
cord-014597-66vd2mdu	1148	7	be	be	VB
cord-014597-66vd2mdu	1148	8	adapted	adapt	VBN
cord-014597-66vd2mdu	1148	9	to	to	IN
cord-014597-66vd2mdu	1148	10	new	new	JJ
cord-014597-66vd2mdu	1148	11	products	product	NNS
cord-014597-66vd2mdu	1148	12	,	,	,
cord-014597-66vd2mdu	1148	13	cell	cell	NN
cord-014597-66vd2mdu	1148	14	lines	line	NNS
cord-014597-66vd2mdu	1148	15	and	and	CC
cord-014597-66vd2mdu	1148	16	clones	clone	NNS
cord-014597-66vd2mdu	1148	17	as	as	RB
cord-014597-66vd2mdu	1148	18	well	well	RB
cord-014597-66vd2mdu	1148	19	as	as	IN
cord-014597-66vd2mdu	1148	20	to	to	IN
cord-014597-66vd2mdu	1148	21	different	different	JJ
cord-014597-66vd2mdu	1148	22	production	production	NN
cord-014597-66vd2mdu	1148	23	plants	plant	NNS
cord-014597-66vd2mdu	1148	24	when	when	WRB
cord-014597-66vd2mdu	1148	25	transferring	transfer	VBG
cord-014597-66vd2mdu	1148	26	processes	process	NNS
cord-014597-66vd2mdu	1148	27	.	.	.
cord-014597-66vd2mdu	1149	1	One	one	CD
cord-014597-66vd2mdu	1149	2	approach	approach	NN
cord-014597-66vd2mdu	1149	3	to	to	TO
cord-014597-66vd2mdu	1149	4	encounter	encounter	VB
cord-014597-66vd2mdu	1149	5	the	the	DT
cord-014597-66vd2mdu	1149	6	variabilities	variability	NNS
cord-014597-66vd2mdu	1149	7	and	and	CC
cord-014597-66vd2mdu	1149	8	to	to	TO
cord-014597-66vd2mdu	1149	9	include	include	VB
cord-014597-66vd2mdu	1149	10	actual	actual	JJ
cord-014597-66vd2mdu	1149	11	information	information	NN
cord-014597-66vd2mdu	1149	12	from	from	IN
cord-014597-66vd2mdu	1149	13	the	the	DT
cord-014597-66vd2mdu	1149	14	process	process	NN
cord-014597-66vd2mdu	1149	15	and	and	CC
cord-014597-66vd2mdu	1149	16	from	from	IN
cord-014597-66vd2mdu	1149	17	data	datum	NNS
cord-014597-66vd2mdu	1149	18	analysis	analysis	NN
cord-014597-66vd2mdu	1149	19	is	be	VBZ
cord-014597-66vd2mdu	1149	20	adaptive	adaptive	JJ
cord-014597-66vd2mdu	1149	21	model	model	NN
cord-014597-66vd2mdu	1149	22	-	-	HYPH
cord-014597-66vd2mdu	1149	23	assisted	assist	VBN
cord-014597-66vd2mdu	1149	24	control	control	NN
cord-014597-66vd2mdu	1149	25	[	[	-LRB-
cord-014597-66vd2mdu	1149	26	1	1	CD
cord-014597-66vd2mdu	1149	27	]	]	-RRB-
cord-014597-66vd2mdu	1149	28	.	.	.
cord-014597-66vd2mdu	1150	1	Two	two	CD
cord-014597-66vd2mdu	1150	2	software	software	NN
cord-014597-66vd2mdu	1150	3	tools	tool	NNS
cord-014597-66vd2mdu	1150	4	enabling	enable	VBG
cord-014597-66vd2mdu	1150	5	adaptive	adaptive	JJ
cord-014597-66vd2mdu	1150	6	model	model	NN
cord-014597-66vd2mdu	1150	7	-	-	HYPH
cord-014597-66vd2mdu	1150	8	assisted	assist	VBN
cord-014597-66vd2mdu	1150	9	control	control	NN
cord-014597-66vd2mdu	1150	10	applying	apply	VBG
cord-014597-66vd2mdu	1150	11	unstructured	unstructured	JJ
cord-014597-66vd2mdu	1150	12	,	,	,
cord-014597-66vd2mdu	1150	13	unsegregated	unsegregated	JJ
cord-014597-66vd2mdu	1150	14	models	model	NNS
cord-014597-66vd2mdu	1150	15	have	have	VBP
cord-014597-66vd2mdu	1150	16	been	be	VBN
cord-014597-66vd2mdu	1150	17	developed	develop	VBN
cord-014597-66vd2mdu	1150	18	and	and	CC
cord-014597-66vd2mdu	1150	19	implemented	implement	VBN
cord-014597-66vd2mdu	1150	20	using	use	VBG
cord-014597-66vd2mdu	1150	21	MATLAB	MATLAB	NNP
cord-014597-66vd2mdu	1150	22	©	©	NNP
cord-014597-66vd2mdu	1150	23	,	,	,
cord-014597-66vd2mdu	1150	24	WinErs	WinErs	NNP
cord-014597-66vd2mdu	1150	25	and	and	CC
cord-014597-66vd2mdu	1150	26	Fortran	Fortran	NNP
cord-014597-66vd2mdu	1150	27	,	,	,
cord-014597-66vd2mdu	1150	28	one	one	CD
cord-014597-66vd2mdu	1150	29	tool	tool	NN
cord-014597-66vd2mdu	1150	30	for	for	IN
cord-014597-66vd2mdu	1150	31	fedbatch	fedbatch	NN
cord-014597-66vd2mdu	1150	32	control	control	NN
cord-014597-66vd2mdu	1150	33	and	and	CC
cord-014597-66vd2mdu	1150	34	another	another	DT
cord-014597-66vd2mdu	1150	35	one	one	NN
cord-014597-66vd2mdu	1150	36	for	for	IN
cord-014597-66vd2mdu	1150	37	seed	seed	NN
cord-014597-66vd2mdu	1150	38	train	train	NN
cord-014597-66vd2mdu	1150	39	simulation	simulation	NN
cord-014597-66vd2mdu	1150	40	and	and	CC
cord-014597-66vd2mdu	1150	41	optimization	optimization	NN
cord-014597-66vd2mdu	1150	42	.	.	.
cord-014597-66vd2mdu	1151	1	One	one	CD
cord-014597-66vd2mdu	1151	2	key	key	JJ
cord-014597-66vd2mdu	1151	3	element	element	NN
cord-014597-66vd2mdu	1151	4	of	of	IN
cord-014597-66vd2mdu	1151	5	adaptive	adaptive	JJ
cord-014597-66vd2mdu	1151	6	model	model	NN
cord-014597-66vd2mdu	1151	7	-	-	HYPH
cord-014597-66vd2mdu	1151	8	assisted	assist	VBN
cord-014597-66vd2mdu	1151	9	control	control	NN
cord-014597-66vd2mdu	1151	10	is	be	VBZ
cord-014597-66vd2mdu	1151	11	the	the	DT
cord-014597-66vd2mdu	1151	12	underlying	underlying	JJ
cord-014597-66vd2mdu	1151	13	process	process	NN
cord-014597-66vd2mdu	1151	14	model	model	NN
cord-014597-66vd2mdu	1151	15	.	.	.
cord-014597-66vd2mdu	1152	1	In	in	IN
cord-014597-66vd2mdu	1152	2	order	order	NN
cord-014597-66vd2mdu	1152	3	to	to	TO
cord-014597-66vd2mdu	1152	4	provide	provide	VB
cord-014597-66vd2mdu	1152	5	an	an	DT
cord-014597-66vd2mdu	1152	6	adaptive	adaptive	JJ
cord-014597-66vd2mdu	1152	7	character	character	NN
cord-014597-66vd2mdu	1152	8	,	,	,
cord-014597-66vd2mdu	1152	9	model	model	NN
cord-014597-66vd2mdu	1152	10	parameters	parameter	NNS
cord-014597-66vd2mdu	1152	11	should	should	MD
cord-014597-66vd2mdu	1152	12	be	be	VB
cord-014597-66vd2mdu	1152	13	easily	easily	RB
cord-014597-66vd2mdu	1152	14	identifiable	identifiable	JJ
cord-014597-66vd2mdu	1152	15	from	from	IN
cord-014597-66vd2mdu	1152	16	routine	routine	JJ
cord-014597-66vd2mdu	1152	17	cultivation	cultivation	NN
cord-014597-66vd2mdu	1152	18	data	datum	NNS
cord-014597-66vd2mdu	1152	19	,	,	,
cord-014597-66vd2mdu	1152	20	which	which	WDT
cord-014597-66vd2mdu	1152	21	is	be	VBZ
cord-014597-66vd2mdu	1152	22	available	available	JJ
cord-014597-66vd2mdu	1152	23	during	during	IN
cord-014597-66vd2mdu	1152	24	seed	seed	NN
cord-014597-66vd2mdu	1152	25	train	train	NN
cord-014597-66vd2mdu	1152	26	and	and	CC
cord-014597-66vd2mdu	1152	27	fed	fed	NN
cord-014597-66vd2mdu	1152	28	-	-	HYPH
cord-014597-66vd2mdu	1152	29	batch	batch	NN
cord-014597-66vd2mdu	1152	30	without	without	IN
cord-014597-66vd2mdu	1152	31	additional	additional	JJ
cord-014597-66vd2mdu	1152	32	sophisticated	sophisticated	JJ
cord-014597-66vd2mdu	1152	33	measurements	measurement	NNS
cord-014597-66vd2mdu	1152	34	.	.	.
cord-014597-66vd2mdu	1153	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1153	2	,	,	,
cord-014597-66vd2mdu	1153	3	the	the	DT
cord-014597-66vd2mdu	1153	4	usage	usage	NN
cord-014597-66vd2mdu	1153	5	of	of	IN
cord-014597-66vd2mdu	1153	6	unstructured	unstructured	JJ
cord-014597-66vd2mdu	1153	7	,	,	,
cord-014597-66vd2mdu	1153	8	unsegregated	unsegregated	JJ
cord-014597-66vd2mdu	1153	9	models	model	NNS
cord-014597-66vd2mdu	1153	10	is	be	VBZ
cord-014597-66vd2mdu	1153	11	recommended	recommend	VBN
cord-014597-66vd2mdu	1153	12	.	.	.
cord-014597-66vd2mdu	1154	1	a	a	LS
cord-014597-66vd2mdu	1154	2	)	)	-RRB-
cord-014597-66vd2mdu	1155	1	Example	example	NN
cord-014597-66vd2mdu	1155	2	of	of	IN
cord-014597-66vd2mdu	1155	3	an	an	DT
cord-014597-66vd2mdu	1155	4	unstructured	unstructured	JJ
cord-014597-66vd2mdu	1155	5	,	,	,
cord-014597-66vd2mdu	1155	6	unsegregated	unsegregated	JJ
cord-014597-66vd2mdu	1155	7	cell	cell	NN
cord-014597-66vd2mdu	1155	8	culture	culture	NN
cord-014597-66vd2mdu	1155	9	model	model	NN
cord-014597-66vd2mdu	1155	10	(	(	-LRB-
cord-014597-66vd2mdu	1155	11	for	for	IN
cord-014597-66vd2mdu	1155	12	adaptive	adaptive	JJ
cord-014597-66vd2mdu	1155	13	model	model	NN
cord-014597-66vd2mdu	1155	14	-	-	HYPH
cord-014597-66vd2mdu	1155	15	assisted	assist	VBN
cord-014597-66vd2mdu	1155	16	control	control	NN
cord-014597-66vd2mdu	1155	17	)	)	-RRB-
cord-014597-66vd2mdu	1156	1	One	one	CD
cord-014597-66vd2mdu	1156	2	example	example	NN
cord-014597-66vd2mdu	1156	3	,	,	,
cord-014597-66vd2mdu	1156	4	describing	describe	VBG
cord-014597-66vd2mdu	1156	5	cell	cell	NN
cord-014597-66vd2mdu	1156	6	growth	growth	NN
cord-014597-66vd2mdu	1156	7	,	,	,
cord-014597-66vd2mdu	1156	8	cell	cell	NN
cord-014597-66vd2mdu	1156	9	death	death	NN
cord-014597-66vd2mdu	1156	10	,	,	,
cord-014597-66vd2mdu	1156	11	uptake	uptake	NN
cord-014597-66vd2mdu	1156	12	of	of	IN
cord-014597-66vd2mdu	1156	13	substrates	substrate	NNS
cord-014597-66vd2mdu	1156	14	and	and	CC
cord-014597-66vd2mdu	1156	15	production	production	NN
cord-014597-66vd2mdu	1156	16	of	of	IN
cord-014597-66vd2mdu	1156	17	metabolites	metabolite	NNS
cord-014597-66vd2mdu	1156	18	via	via	IN
cord-014597-66vd2mdu	1156	19	a	a	DT
cord-014597-66vd2mdu	1156	20	first	first	JJ
cord-014597-66vd2mdu	1156	21	order	order	NN
cord-014597-66vd2mdu	1156	22	system	system	NN
cord-014597-66vd2mdu	1156	23	of	of	IN
cord-014597-66vd2mdu	1156	24	ordinary	ordinary	JJ
cord-014597-66vd2mdu	1156	25	differential	differential	JJ
cord-014597-66vd2mdu	1156	26	equations	equation	NNS
cord-014597-66vd2mdu	1156	27	and	and	CC
cord-014597-66vd2mdu	1156	28	Monod	Monod	NNP
cord-014597-66vd2mdu	1156	29	-	-	HYPH
cord-014597-66vd2mdu	1156	30	type	type	NN
cord-014597-66vd2mdu	1156	31	kinetics	kinetics	NN
cord-014597-66vd2mdu	1156	32	,	,	,
cord-014597-66vd2mdu	1156	33	is	be	VBZ
cord-014597-66vd2mdu	1156	34	shown	show	VBN
cord-014597-66vd2mdu	1156	35	in	in	IN
cord-014597-66vd2mdu	1156	36	Table	Table	NNP
cord-014597-66vd2mdu	1156	37	1	1	CD
cord-014597-66vd2mdu	1156	38	.	.	.
cord-014597-66vd2mdu	1157	1	This	this	DT
cord-014597-66vd2mdu	1157	2	mathematical	mathematical	JJ
cord-014597-66vd2mdu	1157	3	model	model	NN
cord-014597-66vd2mdu	1157	4	includes	include	VBZ
cord-014597-66vd2mdu	1157	5	13	13	CD
cord-014597-66vd2mdu	1157	6	cell	cell	NN
cord-014597-66vd2mdu	1157	7	specific	specific	JJ
cord-014597-66vd2mdu	1157	8	model	model	NN
cord-014597-66vd2mdu	1157	9	parameters	parameter	NNS
cord-014597-66vd2mdu	1157	10	[	[	-LRB-
cord-014597-66vd2mdu	1157	11	2	2	CD
cord-014597-66vd2mdu	1157	12	]	]	-RRB-
cord-014597-66vd2mdu	1157	13	.	.	.
cord-014597-66vd2mdu	1158	1	ii	ii	LS
cord-014597-66vd2mdu	1158	2	)	)	-RRB-
cord-014597-66vd2mdu	1158	3	b	b	LS
cord-014597-66vd2mdu	1158	4	)	)	-RRB-
cord-014597-66vd2mdu	1158	5	Open	open	JJ
cord-014597-66vd2mdu	1158	6	-	-	HYPH
cord-014597-66vd2mdu	1158	7	loop	loop	NN
cord-014597-66vd2mdu	1158	8	control	control	NN
cord-014597-66vd2mdu	1158	9	sequence	sequence	NN
cord-014597-66vd2mdu	1158	10	for	for	IN
cord-014597-66vd2mdu	1158	11	seed	seed	NN
cord-014597-66vd2mdu	1158	12	train	train	NN
cord-014597-66vd2mdu	1158	13	simulation	simulation	NN
cord-014597-66vd2mdu	1158	14	and	and	CC
cord-014597-66vd2mdu	1158	15	optimization	optimization	NN
cord-014597-66vd2mdu	1158	16	[	[	-LRB-
cord-014597-66vd2mdu	1158	17	3	3	CD
cord-014597-66vd2mdu	1158	18	]	]	-RRB-
cord-014597-66vd2mdu	1158	19	:	:	:
cord-014597-66vd2mdu	1159	1	Using	use	VBG
cord-014597-66vd2mdu	1159	2	model	model	NN
cord-014597-66vd2mdu	1159	3	,	,	,
cord-014597-66vd2mdu	1159	4	a	a	FW
cord-014597-66vd2mdu	1159	5	priori	priori	FW
cord-014597-66vd2mdu	1159	6	identified	identify	VBN
cord-014597-66vd2mdu	1159	7	model	model	NN
cord-014597-66vd2mdu	1159	8	parameters	parameter	NNS
cord-014597-66vd2mdu	1159	9	and	and	CC
cord-014597-66vd2mdu	1159	10	starting	start	VBG
cord-014597-66vd2mdu	1159	11	concentration	concentration	NN
cord-014597-66vd2mdu	1159	12	values	value	NNS
cord-014597-66vd2mdu	1159	13	,	,	,
cord-014597-66vd2mdu	1159	14	the	the	DT
cord-014597-66vd2mdu	1159	15	temporal	temporal	JJ
cord-014597-66vd2mdu	1159	16	concentration	concentration	NN
cord-014597-66vd2mdu	1159	17	courses	course	NNS
cord-014597-66vd2mdu	1159	18	can	can	MD
cord-014597-66vd2mdu	1159	19	be	be	VB
cord-014597-66vd2mdu	1159	20	predicted	predict	VBN
cord-014597-66vd2mdu	1159	21	for	for	IN
cord-014597-66vd2mdu	1159	22	the	the	DT
cord-014597-66vd2mdu	1159	23	first	first	JJ
cord-014597-66vd2mdu	1159	24	scale	scale	NN
cord-014597-66vd2mdu	1159	25	.	.	.
cord-014597-66vd2mdu	1160	1	Subsequently	subsequently	RB
cord-014597-66vd2mdu	1160	2	,	,	,
cord-014597-66vd2mdu	1160	3	points	point	NNS
cord-014597-66vd2mdu	1160	4	in	in	IN
cord-014597-66vd2mdu	1160	5	time	time	NN
cord-014597-66vd2mdu	1160	6	for	for	IN
cord-014597-66vd2mdu	1160	7	passaging	passaging	NN
cord-014597-66vd2mdu	1160	8	and	and	CC
cord-014597-66vd2mdu	1160	9	starting	start	VBG
cord-014597-66vd2mdu	1160	10	values	value	NNS
cord-014597-66vd2mdu	1160	11	for	for	IN
cord-014597-66vd2mdu	1160	12	the	the	DT
cord-014597-66vd2mdu	1160	13	next	next	JJ
cord-014597-66vd2mdu	1160	14	scale	scale	NN
cord-014597-66vd2mdu	1160	15	can	can	MD
cord-014597-66vd2mdu	1160	16	be	be	VB
cord-014597-66vd2mdu	1160	17	computed	compute	VBN
cord-014597-66vd2mdu	1160	18	by	by	IN
cord-014597-66vd2mdu	1160	19	adding	add	VBG
cord-014597-66vd2mdu	1160	20	a	a	DT
cord-014597-66vd2mdu	1160	21	passaging	passaging	NN
cord-014597-66vd2mdu	1160	22	strategy	strategy	NN
cord-014597-66vd2mdu	1160	23	,	,	,
cord-014597-66vd2mdu	1160	24	seed	seed	NN
cord-014597-66vd2mdu	1160	25	train	train	NN
cord-014597-66vd2mdu	1160	26	conditions	condition	NNS
cord-014597-66vd2mdu	1160	27	and	and	CC
cord-014597-66vd2mdu	1160	28	medium	medium	NN
cord-014597-66vd2mdu	1160	29	concentrations	concentration	NNS
cord-014597-66vd2mdu	1160	30	.	.	.
cord-014597-66vd2mdu	1161	1	Prediction	prediction	NN
cord-014597-66vd2mdu	1161	2	for	for	IN
cord-014597-66vd2mdu	1161	3	the	the	DT
cord-014597-66vd2mdu	1161	4	following	follow	VBG
cord-014597-66vd2mdu	1161	5	scales	scale	NNS
cord-014597-66vd2mdu	1161	6	can	can	MD
cord-014597-66vd2mdu	1161	7	be	be	VB
cord-014597-66vd2mdu	1161	8	obtained	obtain	VBN
cord-014597-66vd2mdu	1161	9	iteratively	iteratively	RB
cord-014597-66vd2mdu	1161	10	.	.	.
cord-014597-66vd2mdu	1162	1	Integrating	integrate	VBG
cord-014597-66vd2mdu	1162	2	feedback	feedback	NN
cord-014597-66vd2mdu	1162	3	from	from	IN
cord-014597-66vd2mdu	1162	4	the	the	DT
cord-014597-66vd2mdu	1162	5	process	process	NN
cord-014597-66vd2mdu	1162	6	in	in	IN
cord-014597-66vd2mdu	1162	7	terms	term	NNS
cord-014597-66vd2mdu	1162	8	of	of	IN
cord-014597-66vd2mdu	1162	9	cultivation	cultivation	NN
cord-014597-66vd2mdu	1162	10	data	datum	NNS
cord-014597-66vd2mdu	1162	11	enables	enable	VBZ
cord-014597-66vd2mdu	1162	12	increasing	increase	VBG
cord-014597-66vd2mdu	1162	13	prediction	prediction	NN
cord-014597-66vd2mdu	1162	14	accuracy	accuracy	NN
cord-014597-66vd2mdu	1162	15	and	and	CC
cord-014597-66vd2mdu	1162	16	responding	respond	VBG
cord-014597-66vd2mdu	1162	17	to	to	IN
cord-014597-66vd2mdu	1162	18	possible	possible	JJ
cord-014597-66vd2mdu	1162	19	changes	change	NNS
cord-014597-66vd2mdu	1162	20	in	in	IN
cord-014597-66vd2mdu	1162	21	cell	cell	NN
cord-014597-66vd2mdu	1162	22	behaviour	behaviour	NN
cord-014597-66vd2mdu	1162	23	.	.	.
cord-014597-66vd2mdu	1163	1	Process	process	NN
cord-014597-66vd2mdu	1163	2	design	design	NN
cord-014597-66vd2mdu	1163	3	and	and	CC
cord-014597-66vd2mdu	1163	4	optimization	optimization	NN
cord-014597-66vd2mdu	1163	5	,	,	,
cord-014597-66vd2mdu	1164	1	e.g.	e.g.	RB
cord-014597-66vd2mdu	1164	2	regarding	regard	VBG
cord-014597-66vd2mdu	1164	3	seed	seed	NN
cord-014597-66vd2mdu	1164	4	train	train	NN
cord-014597-66vd2mdu	1164	5	and	and	CC
cord-014597-66vd2mdu	1164	6	fed	fed	NN
cord-014597-66vd2mdu	1164	7	-	-	HYPH
cord-014597-66vd2mdu	1164	8	batch	batch	NN
cord-014597-66vd2mdu	1164	9	,	,	,
cord-014597-66vd2mdu	1164	10	is	be	VBZ
cord-014597-66vd2mdu	1164	11	realized	realize	VBN
cord-014597-66vd2mdu	1164	12	by	by	IN
cord-014597-66vd2mdu	1164	13	adaptive	adaptive	JJ
cord-014597-66vd2mdu	1164	14	model	model	NN
cord-014597-66vd2mdu	1164	15	-	-	HYPH
cord-014597-66vd2mdu	1164	16	assisted	assist	VBN
cord-014597-66vd2mdu	1164	17	software	software	NN
cord-014597-66vd2mdu	1164	18	tools	tool	NNS
cord-014597-66vd2mdu	1164	19	using	use	VBG
cord-014597-66vd2mdu	1164	20	unstructured	unstructured	JJ
cord-014597-66vd2mdu	1164	21	,	,	,
cord-014597-66vd2mdu	1164	22	unsegregated	unsegregated	JJ
cord-014597-66vd2mdu	1164	23	models	model	NNS
cord-014597-66vd2mdu	1164	24	.	.	.
cord-014597-66vd2mdu	1165	1	They	-PRON-	PRP
cord-014597-66vd2mdu	1165	2	enable	enable	VBP
cord-014597-66vd2mdu	1165	3	feedback	feedback	NN
cord-014597-66vd2mdu	1165	4	from	from	IN
cord-014597-66vd2mdu	1165	5	the	the	DT
cord-014597-66vd2mdu	1165	6	process	process	NN
cord-014597-66vd2mdu	1165	7	via	via	IN
cord-014597-66vd2mdu	1165	8	routine	routine	JJ
cord-014597-66vd2mdu	1165	9	cultivation	cultivation	NN
cord-014597-66vd2mdu	1165	10	data	datum	NNS
cord-014597-66vd2mdu	1165	11	and	and	CC
cord-014597-66vd2mdu	1165	12	allow	allow	VB
cord-014597-66vd2mdu	1165	13	adaptation	adaptation	NN
cord-014597-66vd2mdu	1165	14	to	to	IN
cord-014597-66vd2mdu	1165	15	diverse	diverse	JJ
cord-014597-66vd2mdu	1165	16	circumstances	circumstance	NNS
cord-014597-66vd2mdu	1165	17	such	such	JJ
cord-014597-66vd2mdu	1165	18	as	as	IN
cord-014597-66vd2mdu	1165	19	different	different	JJ
cord-014597-66vd2mdu	1165	20	cell	cell	NN
cord-014597-66vd2mdu	1165	21	lines	line	NNS
cord-014597-66vd2mdu	1165	22	,	,	,
cord-014597-66vd2mdu	1165	23	products	product	NNS
cord-014597-66vd2mdu	1165	24	,	,	,
cord-014597-66vd2mdu	1165	25	cultivation	cultivation	NN
cord-014597-66vd2mdu	1165	26	conditions	condition	NNS
cord-014597-66vd2mdu	1165	27	,	,	,
cord-014597-66vd2mdu	1165	28	plant	plant	NN
cord-014597-66vd2mdu	1165	29	configurations	configuration	NNS
cord-014597-66vd2mdu	1165	30	etc	etc	FW
cord-014597-66vd2mdu	1165	31	.	.	.
cord-014597-66vd2mdu	1165	32	)	)	-RRB-
cord-014597-66vd2mdu	1166	1	in	in	IN
cord-014597-66vd2mdu	1166	2	polyelectrolyte	polyelectrolyte	NNP
cord-014597-66vd2mdu	1166	3	capsules	capsule	NNS
cord-014597-66vd2mdu	1166	4	.	.	.
cord-014597-66vd2mdu	1167	1	Significant	significant	JJ
cord-014597-66vd2mdu	1167	2	advantages	advantage	NNS
cord-014597-66vd2mdu	1167	3	,	,	,
cord-014597-66vd2mdu	1167	4	such	such	JJ
cord-014597-66vd2mdu	1167	5	as	as	IN
cord-014597-66vd2mdu	1167	6	great	great	JJ
cord-014597-66vd2mdu	1167	7	mechanical	mechanical	JJ
cord-014597-66vd2mdu	1167	8	stability	stability	NN
cord-014597-66vd2mdu	1167	9	,	,	,
cord-014597-66vd2mdu	1167	10	good	good	JJ
cord-014597-66vd2mdu	1167	11	biocompatibility	biocompatibility	NN
cord-014597-66vd2mdu	1167	12	and	and	CC
cord-014597-66vd2mdu	1167	13	good	good	JJ
cord-014597-66vd2mdu	1167	14	mass	mass	NN
cord-014597-66vd2mdu	1167	15	transfer	transfer	NN
cord-014597-66vd2mdu	1167	16	properties	property	NNS
cord-014597-66vd2mdu	1167	17	characterized	characterize	VBD
cord-014597-66vd2mdu	1167	18	these	these	DT
cord-014597-66vd2mdu	1167	19	capsules	capsule	NNS
cord-014597-66vd2mdu	1167	20	based	base	VBN
cord-014597-66vd2mdu	1167	21	on	on	IN
cord-014597-66vd2mdu	1167	22	sodium	sodium	NN
cord-014597-66vd2mdu	1167	23	cellulose	cellulose	NN
cord-014597-66vd2mdu	1167	24	sulfate	sulfate	NN
cord-014597-66vd2mdu	1167	25	/	/	SYM
cord-014597-66vd2mdu	1167	26	poly(diallyldimethyl	poly(diallyldimethyl	NN
cord-014597-66vd2mdu	1167	27	)	)	-RRB-
cord-014597-66vd2mdu	1168	1	ammonium	ammonium	NN
cord-014597-66vd2mdu	1168	2	chloride	chloride	NN
cord-014597-66vd2mdu	1168	3	(	(	-LRB-
cord-014597-66vd2mdu	1168	4	SCS	SCS	NNP
cord-014597-66vd2mdu	1168	5	/	/	SYM
cord-014597-66vd2mdu	1168	6	PDADMAC	PDADMAC	NNP
cord-014597-66vd2mdu	1168	7	)	)	-RRB-
cord-014597-66vd2mdu	1169	1	[	[	-LRB-
cord-014597-66vd2mdu	1169	2	1	1	CD
cord-014597-66vd2mdu	1169	3	,	,	,
cord-014597-66vd2mdu	1169	4	2	2	CD
cord-014597-66vd2mdu	1169	5	]	]	-RRB-
cord-014597-66vd2mdu	1169	6	.	.	.
cord-014597-66vd2mdu	1170	1	Here	here	RB
cord-014597-66vd2mdu	1170	2	,	,	,
cord-014597-66vd2mdu	1170	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1170	4	present	present	VBP
cord-014597-66vd2mdu	1170	5	the	the	DT
cord-014597-66vd2mdu	1170	6	possibility	possibility	NN
cord-014597-66vd2mdu	1170	7	to	to	TO
cord-014597-66vd2mdu	1170	8	cultivate	cultivate	VB
cord-014597-66vd2mdu	1170	9	human	human	JJ
cord-014597-66vd2mdu	1170	10	T	t	NN
cord-014597-66vd2mdu	1170	11	cells	cell	NNS
cord-014597-66vd2mdu	1170	12	,	,	,
cord-014597-66vd2mdu	1170	13	freshly	freshly	RB
cord-014597-66vd2mdu	1170	14	isolated	isolate	VBN
cord-014597-66vd2mdu	1170	15	from	from	IN
cord-014597-66vd2mdu	1170	16	blood	blood	NN
cord-014597-66vd2mdu	1170	17	,	,	,
cord-014597-66vd2mdu	1170	18	to	to	IN
cord-014597-66vd2mdu	1170	19	high	high	JJ
cord-014597-66vd2mdu	1170	20	densities	density	NNS
cord-014597-66vd2mdu	1170	21	in	in	IN
cord-014597-66vd2mdu	1170	22	similar	similar	JJ
cord-014597-66vd2mdu	1170	23	semipermeable	semipermeable	JJ
cord-014597-66vd2mdu	1170	24	polyelectrolyte	polyelectrolyte	NN
cord-014597-66vd2mdu	1170	25	microcapsules	microcapsules	NNP
cord-014597-66vd2mdu	1170	26	within	within	IN
cord-014597-66vd2mdu	1170	27	less	less	JJR
cord-014597-66vd2mdu	1170	28	than	than	IN
cord-014597-66vd2mdu	1170	29	10	10	CD
cord-014597-66vd2mdu	1170	30	days	day	NNS
cord-014597-66vd2mdu	1170	31	.	.	.
cord-014597-66vd2mdu	1171	1	Cells	cell	NNS
cord-014597-66vd2mdu	1171	2	were	be	VBD
cord-014597-66vd2mdu	1171	3	encapsulated	encapsulate	VBN
cord-014597-66vd2mdu	1171	4	in	in	IN
cord-014597-66vd2mdu	1171	5	semipermeable	semipermeable	JJ
cord-014597-66vd2mdu	1171	6	SCS	SCS	NNP
cord-014597-66vd2mdu	1171	7	/	/	SYM
cord-014597-66vd2mdu	1171	8	PDADMAC	PDADMAC	NNP
cord-014597-66vd2mdu	1171	9	polyelectrolyte	polyelectrolyte	NN
cord-014597-66vd2mdu	1171	10	microcapsules	microcapsule	NNS
cord-014597-66vd2mdu	1171	11	or	or	CC
cord-014597-66vd2mdu	1171	12	confined	confine	VBN
cord-014597-66vd2mdu	1171	13	in	in	IN
cord-014597-66vd2mdu	1171	14	1.5	1.5	CD
cord-014597-66vd2mdu	1171	15	%	%	NN
cord-014597-66vd2mdu	1171	16	alginate	alginate	NN
cord-014597-66vd2mdu	1171	17	/	/	SYM
cord-014597-66vd2mdu	1171	18	poly	poly	JJ
cord-014597-66vd2mdu	1171	19	-	-	HYPH
cord-014597-66vd2mdu	1171	20	l	l	NN
cord-014597-66vd2mdu	1171	21	-	-	HYPH
cord-014597-66vd2mdu	1171	22	lysine	lysine	NN
cord-014597-66vd2mdu	1171	23	(	(	-LRB-
cord-014597-66vd2mdu	1171	24	PLL	PLL	NNP
cord-014597-66vd2mdu	1171	25	)	)	-RRB-
cord-014597-66vd2mdu	1171	26	beads	bead	NNS
cord-014597-66vd2mdu	1171	27	,	,	,
cord-014597-66vd2mdu	1171	28	a	a	DT
cord-014597-66vd2mdu	1171	29	standard	standard	JJ
cord-014597-66vd2mdu	1171	30	approach	approach	NN
cord-014597-66vd2mdu	1171	31	for	for	IN
cord-014597-66vd2mdu	1171	32	cell	cell	NN
cord-014597-66vd2mdu	1171	33	immobilization	immobilization	NN
cord-014597-66vd2mdu	1171	34	.	.	.
cord-014597-66vd2mdu	1172	1	The	the	DT
cord-014597-66vd2mdu	1172	2	permeability	permeability	NN
cord-014597-66vd2mdu	1172	3	of	of	IN
cord-014597-66vd2mdu	1172	4	the	the	DT
cord-014597-66vd2mdu	1172	5	microcapsules	microcapsule	NNS
cord-014597-66vd2mdu	1172	6	was	be	VBD
cord-014597-66vd2mdu	1172	7	estimated	estimate	VBN
cord-014597-66vd2mdu	1172	8	using	use	VBG
cord-014597-66vd2mdu	1172	9	dextran	dextran	NN
cord-014597-66vd2mdu	1172	10	-	-	HYPH
cord-014597-66vd2mdu	1172	11	based	base	VBN
cord-014597-66vd2mdu	1172	12	molecular	molecular	JJ
cord-014597-66vd2mdu	1172	13	weight	weight	NN
cord-014597-66vd2mdu	1172	14	standards	standard	NNS
cord-014597-66vd2mdu	1172	15	(	(	-LRB-
cord-014597-66vd2mdu	1172	16	10	10	CD
cord-014597-66vd2mdu	1172	17	and	and	CC
cord-014597-66vd2mdu	1172	18	20	20	CD
cord-014597-66vd2mdu	1172	19	kDa	kDa	NNS
cord-014597-66vd2mdu	1172	20	)	)	-RRB-
cord-014597-66vd2mdu	1172	21	and	and	CC
cord-014597-66vd2mdu	1172	22	vitamin	vitamin	NN
cord-014597-66vd2mdu	1172	23	B12	b12	NN
cord-014597-66vd2mdu	1172	24	(	(	-LRB-
cord-014597-66vd2mdu	1172	25	1.6	1.6	CD
cord-014597-66vd2mdu	1172	26	kDa	kDa	NNS
cord-014597-66vd2mdu	1172	27	)	)	-RRB-
cord-014597-66vd2mdu	1172	28	.	.	.
cord-014597-66vd2mdu	1173	1	Gentle	gentle	JJ
cord-014597-66vd2mdu	1173	2	digestion	digestion	NN
cord-014597-66vd2mdu	1173	3	with	with	IN
cord-014597-66vd2mdu	1173	4	endocellulase	endocellulase	NN
cord-014597-66vd2mdu	1173	5	allows	allow	VBZ
cord-014597-66vd2mdu	1173	6	an	an	DT
cord-014597-66vd2mdu	1173	7	easy	easy	JJ
cord-014597-66vd2mdu	1173	8	release	release	NN
cord-014597-66vd2mdu	1173	9	of	of	IN
cord-014597-66vd2mdu	1173	10	the	the	DT
cord-014597-66vd2mdu	1173	11	cells	cell	NNS
cord-014597-66vd2mdu	1173	12	out	out	IN
cord-014597-66vd2mdu	1173	13	of	of	IN
cord-014597-66vd2mdu	1173	14	the	the	DT
cord-014597-66vd2mdu	1173	15	capsules	capsule	NNS
cord-014597-66vd2mdu	1173	16	.	.	.
cord-014597-66vd2mdu	1174	1	Cell	cell	NN
cord-014597-66vd2mdu	1174	2	growth	growth	NN
cord-014597-66vd2mdu	1175	1	,	,	,
cord-014597-66vd2mdu	1175	2	cytokines	cytokine	NNS
cord-014597-66vd2mdu	1175	3	production	production	NN
cord-014597-66vd2mdu	1175	4	and	and	CC
cord-014597-66vd2mdu	1175	5	phenotype	phenotype	NN
cord-014597-66vd2mdu	1175	6	were	be	VBD
cord-014597-66vd2mdu	1175	7	measured	measure	VBN
cord-014597-66vd2mdu	1175	8	in	in	IN
cord-014597-66vd2mdu	1175	9	non	non	JJ
cord-014597-66vd2mdu	1175	10	-	-	JJ
cord-014597-66vd2mdu	1175	11	encapsulated	encapsulated	JJ
cord-014597-66vd2mdu	1175	12	and	and	CC
cord-014597-66vd2mdu	1175	13	encapsulated	encapsulate	VBN
cord-014597-66vd2mdu	1175	14	cells	cell	NNS
cord-014597-66vd2mdu	1175	15	grown	grow	VBN
cord-014597-66vd2mdu	1175	16	under	under	IN
cord-014597-66vd2mdu	1175	17	standard	standard	JJ
cord-014597-66vd2mdu	1175	18	culture	culture	NN
cord-014597-66vd2mdu	1175	19	conditions	condition	NNS
cord-014597-66vd2mdu	1175	20	.	.	.
cord-014597-66vd2mdu	1176	1	Moreover	moreover	RB
cord-014597-66vd2mdu	1176	2	,	,	,
cord-014597-66vd2mdu	1176	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1176	4	analyzed	analyze	VBD
cord-014597-66vd2mdu	1176	5	the	the	DT
cord-014597-66vd2mdu	1176	6	interplay	interplay	NN
cord-014597-66vd2mdu	1176	7	between	between	IN
cord-014597-66vd2mdu	1176	8	the	the	DT
cord-014597-66vd2mdu	1176	9	secreted	secrete	VBN
cord-014597-66vd2mdu	1176	10	cytokines	cytokine	NNS
cord-014597-66vd2mdu	1176	11	and	and	CC
cord-014597-66vd2mdu	1176	12	the	the	DT
cord-014597-66vd2mdu	1176	13	SCS	SCS	NNP
cord-014597-66vd2mdu	1176	14	within	within	IN
cord-014597-66vd2mdu	1176	15	the	the	DT
cord-014597-66vd2mdu	1176	16	capsules	capsule	NNS
cord-014597-66vd2mdu	1176	17	and	and	CC
cord-014597-66vd2mdu	1176	18	its	-PRON-	PRP$
cord-014597-66vd2mdu	1176	19	putative	putative	JJ
cord-014597-66vd2mdu	1176	20	influence	influence	NN
cord-014597-66vd2mdu	1176	21	on	on	IN
cord-014597-66vd2mdu	1176	22	cell	cell	NN
cord-014597-66vd2mdu	1176	23	growth	growth	NN
cord-014597-66vd2mdu	1176	24	.	.	.
cord-014597-66vd2mdu	1177	1	Cells	cell	NNS
cord-014597-66vd2mdu	1177	2	mixed	mix	VBN
cord-014597-66vd2mdu	1177	3	in	in	IN
cord-014597-66vd2mdu	1177	4	the	the	DT
cord-014597-66vd2mdu	1177	5	cellulose	cellulose	NN
cord-014597-66vd2mdu	1177	6	sulfate	sulfate	NN
cord-014597-66vd2mdu	1177	7	solution	solution	NN
cord-014597-66vd2mdu	1177	8	under	under	IN
cord-014597-66vd2mdu	1177	9	physiological	physiological	JJ
cord-014597-66vd2mdu	1177	10	conditions	condition	NNS
cord-014597-66vd2mdu	1177	11	can	can	MD
cord-014597-66vd2mdu	1177	12	be	be	VB
cord-014597-66vd2mdu	1177	13	safely	safely	RB
cord-014597-66vd2mdu	1177	14	trapped	trap	VBN
cord-014597-66vd2mdu	1177	15	within	within	IN
cord-014597-66vd2mdu	1177	16	a	a	DT
cord-014597-66vd2mdu	1177	17	liquid	liquid	JJ
cord-014597-66vd2mdu	1177	18	core	core	NN
cord-014597-66vd2mdu	1177	19	during	during	IN
cord-014597-66vd2mdu	1177	20	capsule	capsule	NN
cord-014597-66vd2mdu	1177	21	formation	formation	NN
cord-014597-66vd2mdu	1177	22	.	.	.
cord-014597-66vd2mdu	1178	1	Encapsulated	encapsulate	VBN
cord-014597-66vd2mdu	1178	2	cells	cell	NNS
cord-014597-66vd2mdu	1178	3	can	can	MD
cord-014597-66vd2mdu	1178	4	reached	reach	VBN
cord-014597-66vd2mdu	1178	5	cell	cell	NN
cord-014597-66vd2mdu	1178	6	densities	density	NNS
cord-014597-66vd2mdu	1178	7	≤	≤	NNP
cord-014597-66vd2mdu	1178	8	40	40	CD
cord-014597-66vd2mdu	1178	9	x	x	SYM
cord-014597-66vd2mdu	1178	10	10	10	CD
cord-014597-66vd2mdu	1178	11	6	6	CD
cord-014597-66vd2mdu	1178	12	cells	cell	NNS
cord-014597-66vd2mdu	1178	13	mL	ml	NN
cord-014597-66vd2mdu	1178	14	capsule	capsule	NN
cord-014597-66vd2mdu	1178	15	-1	-1	NN
cord-014597-66vd2mdu	1178	16	,	,	,
cord-014597-66vd2mdu	1178	17	whereas	whereas	IN
cord-014597-66vd2mdu	1178	18	cells	cell	NNS
cord-014597-66vd2mdu	1178	19	confined	confine	VBN
cord-014597-66vd2mdu	1178	20	in	in	IN
cord-014597-66vd2mdu	1178	21	alginate	alginate	NN
cord-014597-66vd2mdu	1178	22	/	/	SYM
cord-014597-66vd2mdu	1178	23	PLL	pll	JJ
cord-014597-66vd2mdu	1178	24	beads	bead	NNS
cord-014597-66vd2mdu	1178	25	and	and	CC
cord-014597-66vd2mdu	1178	26	non	non	JJ
cord-014597-66vd2mdu	1178	27	-	-	JJ
cord-014597-66vd2mdu	1178	28	encapsulated	encapsulated	JJ
cord-014597-66vd2mdu	1178	29	ones	one	NNS
cord-014597-66vd2mdu	1178	30	reached	reach	VBD
cord-014597-66vd2mdu	1178	31	11.3	11.3	CD
cord-014597-66vd2mdu	1178	32	x	x	SYM
cord-014597-66vd2mdu	1178	33	10	10	CD
cord-014597-66vd2mdu	1178	34	6	6	CD
cord-014597-66vd2mdu	1178	35	cells	cell	NNS
cord-014597-66vd2mdu	1178	36	mL	ml	NN
cord-014597-66vd2mdu	1178	37	bead	bead	NN
cord-014597-66vd2mdu	1178	38	-1	-1	NN
cord-014597-66vd2mdu	1178	39	and	and	CC
cord-014597-66vd2mdu	1178	40	2.4	2.4	CD
cord-014597-66vd2mdu	1178	41	x	x	SYM
cord-014597-66vd2mdu	1178	42	10	10	CD
cord-014597-66vd2mdu	1178	43	6	6	CD
cord-014597-66vd2mdu	1178	44	cells	cell	NNS
cord-014597-66vd2mdu	1178	45	mL	ml	NN
cord-014597-66vd2mdu	1178	46	,	,	,
cord-014597-66vd2mdu	1178	47	respectively	respectively	RB
cord-014597-66vd2mdu	1178	48	.	.	.
cord-014597-66vd2mdu	1179	1	One	one	CD
cord-014597-66vd2mdu	1179	2	major	major	JJ
cord-014597-66vd2mdu	1179	3	advantage	advantage	NN
cord-014597-66vd2mdu	1179	4	of	of	IN
cord-014597-66vd2mdu	1179	5	these	these	DT
cord-014597-66vd2mdu	1179	6	polyelectrolyte	polyelectrolyte	NNP
cord-014597-66vd2mdu	1179	7	microcapsules	microcapsules	NNP
cord-014597-66vd2mdu	1179	8	(	(	-LRB-
cord-014597-66vd2mdu	1179	9	<	<	NNP
cord-014597-66vd2mdu	1179	10	1	1	CD
cord-014597-66vd2mdu	1179	11	mm	mm	NNP
cord-014597-66vd2mdu	1179	12	)	)	-RRB-
cord-014597-66vd2mdu	1179	13	is	be	VBZ
cord-014597-66vd2mdu	1179	14	the	the	DT
cord-014597-66vd2mdu	1179	15	low	low	JJ
cord-014597-66vd2mdu	1179	16	MWCO	MWCO	NNPS
cord-014597-66vd2mdu	1179	17	(	(	-LRB-
cord-014597-66vd2mdu	1179	18	<	<	NNP
cord-014597-66vd2mdu	1179	19	10	10	CD
cord-014597-66vd2mdu	1179	20	kDa	kDa	NNPS
cord-014597-66vd2mdu	1179	21	)	)	-RRB-
cord-014597-66vd2mdu	1180	1	(	(	-LRB-
cord-014597-66vd2mdu	1180	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1180	3	.	.	.
cord-014597-66vd2mdu	1180	4	1a	1a	CD
cord-014597-66vd2mdu	1180	5	-	-	HYPH
cord-014597-66vd2mdu	1180	6	b	b	NN
cord-014597-66vd2mdu	1180	7	)	)	-RRB-
cord-014597-66vd2mdu	1180	8	.	.	.
cord-014597-66vd2mdu	1181	1	This	this	DT
cord-014597-66vd2mdu	1181	2	restricted	restricted	JJ
cord-014597-66vd2mdu	1181	3	permeability	permeability	NN
cord-014597-66vd2mdu	1181	4	allows	allow	VBZ
cord-014597-66vd2mdu	1181	5	for	for	IN
cord-014597-66vd2mdu	1181	6	a	a	DT
cord-014597-66vd2mdu	1181	7	conditioning	conditioning	NN
cord-014597-66vd2mdu	1181	8	of	of	IN
cord-014597-66vd2mdu	1181	9	the	the	DT
cord-014597-66vd2mdu	1181	10	capsule	capsule	NN
cord-014597-66vd2mdu	1181	11	core	core	NN
cord-014597-66vd2mdu	1181	12	by	by	IN
cord-014597-66vd2mdu	1181	13	autocrine	autocrine	JJ
cord-014597-66vd2mdu	1181	14	factors	factor	NNS
cord-014597-66vd2mdu	1181	15	,	,	,
cord-014597-66vd2mdu	1181	16	which	which	WDT
cord-014597-66vd2mdu	1181	17	in	in	IN
cord-014597-66vd2mdu	1181	18	turn	turn	NN
cord-014597-66vd2mdu	1181	19	permits	permit	VBZ
cord-014597-66vd2mdu	1181	20	the	the	DT
cord-014597-66vd2mdu	1181	21	use	use	NN
cord-014597-66vd2mdu	1181	22	of	of	IN
cord-014597-66vd2mdu	1181	23	basal	basal	JJ
cord-014597-66vd2mdu	1181	24	cell	cell	NN
cord-014597-66vd2mdu	1181	25	culture	culture	NN
cord-014597-66vd2mdu	1181	26	medium	medium	NN
cord-014597-66vd2mdu	1181	27	instead	instead	RB
cord-014597-66vd2mdu	1181	28	of	of	IN
cord-014597-66vd2mdu	1181	29	expensive	expensive	JJ
cord-014597-66vd2mdu	1181	30	T	t	NN
cord-014597-66vd2mdu	1181	31	cell	cell	NN
cord-014597-66vd2mdu	1181	32	specialized	specialized	JJ
cord-014597-66vd2mdu	1181	33	media	medium	NNS
cord-014597-66vd2mdu	1181	34	,	,	,
cord-014597-66vd2mdu	1181	35	hence	hence	RB
cord-014597-66vd2mdu	1181	36	does	do	VBZ
cord-014597-66vd2mdu	1181	37	not	not	RB
cord-014597-66vd2mdu	1181	38	necessitate	necessitate	VB
cord-014597-66vd2mdu	1181	39	high	high	JJ
cord-014597-66vd2mdu	1181	40	amounts	amount	NNS
cord-014597-66vd2mdu	1181	41	of	of	IN
cord-014597-66vd2mdu	1181	42	rhIL-2	rhil-2	NN
cord-014597-66vd2mdu	1181	43	and	and	CC
cord-014597-66vd2mdu	1181	44	reduces	reduce	VBZ
cord-014597-66vd2mdu	1181	45	the	the	DT
cord-014597-66vd2mdu	1181	46	cultivation	cultivation	NN
cord-014597-66vd2mdu	1181	47	costs	cost	NNS
cord-014597-66vd2mdu	1181	48	.	.	.
cord-014597-66vd2mdu	1182	1	Moreover	moreover	RB
cord-014597-66vd2mdu	1182	2	,	,	,
cord-014597-66vd2mdu	1182	3	co	co	NN
cord-014597-66vd2mdu	1182	4	-	-	NN
cord-014597-66vd2mdu	1182	5	encapsulation	encapsulation	NN
cord-014597-66vd2mdu	1182	6	of	of	IN
cord-014597-66vd2mdu	1182	7	rhIL-2	rhil-2	NN
cord-014597-66vd2mdu	1182	8	had	have	VBD
cord-014597-66vd2mdu	1182	9	a	a	DT
cord-014597-66vd2mdu	1182	10	beneficial	beneficial	JJ
cord-014597-66vd2mdu	1182	11	effect	effect	NN
cord-014597-66vd2mdu	1182	12	on	on	IN
cord-014597-66vd2mdu	1182	13	the	the	DT
cord-014597-66vd2mdu	1182	14	growth	growth	NN
cord-014597-66vd2mdu	1182	15	kinetics	kinetic	NNS
cord-014597-66vd2mdu	1182	16	in	in	IN
cord-014597-66vd2mdu	1182	17	most	most	JJS
cord-014597-66vd2mdu	1182	18	cases	case	NNS
cord-014597-66vd2mdu	1183	1	(	(	-LRB-
cord-014597-66vd2mdu	1183	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1183	3	.	.	.
cord-014597-66vd2mdu	1183	4	1c	1c	CD
cord-014597-66vd2mdu	1183	5	)	)	-RRB-
cord-014597-66vd2mdu	1183	6	.	.	.
cord-014597-66vd2mdu	1184	1	Some	some	DT
cord-014597-66vd2mdu	1184	2	evidence	evidence	NN
cord-014597-66vd2mdu	1184	3	is	be	VBZ
cord-014597-66vd2mdu	1184	4	presented	present	VBN
cord-014597-66vd2mdu	1184	5	that	that	IN
cord-014597-66vd2mdu	1184	6	the	the	DT
cord-014597-66vd2mdu	1184	7	SCS	SCS	NNP
cord-014597-66vd2mdu	1184	8	used	use	VBD
cord-014597-66vd2mdu	1184	9	to	to	TO
cord-014597-66vd2mdu	1184	10	form	form	VB
cord-014597-66vd2mdu	1184	11	the	the	DT
cord-014597-66vd2mdu	1184	12	polyelectrolyte	polyelectrolyte	NNP
cord-014597-66vd2mdu	1184	13	microcapsules	microcapsules	NNP
cord-014597-66vd2mdu	1184	14	,	,	,
cord-014597-66vd2mdu	1184	15	specifically	specifically	RB
cord-014597-66vd2mdu	1184	16	adsorbs	adsorb	VBZ
cord-014597-66vd2mdu	1184	17	IL-2	IL-2	NNP
cord-014597-66vd2mdu	1184	18	(	(	-LRB-
cord-014597-66vd2mdu	1184	19	Table	Table	NNP
cord-014597-66vd2mdu	1184	20	1	1	CD
cord-014597-66vd2mdu	1184	21	)	)	-RRB-
cord-014597-66vd2mdu	1185	1	-A	-A	:
cord-014597-66vd2mdu	1185	2	cytokine	cytokine	NN
cord-014597-66vd2mdu	1185	3	which	which	WDT
cord-014597-66vd2mdu	1185	4	provides	provide	VBZ
cord-014597-66vd2mdu	1185	5	an	an	DT
cord-014597-66vd2mdu	1185	6	essential	essential	JJ
cord-014597-66vd2mdu	1185	7	signal	signal	NN
cord-014597-66vd2mdu	1185	8	for	for	IN
cord-014597-66vd2mdu	1185	9	T	t	NN
cord-014597-66vd2mdu	1185	10	-	-	HYPH
cord-014597-66vd2mdu	1185	11	cell	cell	NN
cord-014597-66vd2mdu	1185	12	proliferation	proliferation	NN
cord-014597-66vd2mdu	1185	13	and	and	CC
cord-014597-66vd2mdu	1185	14	differentiation	differentiation	NN
cord-014597-66vd2mdu	1185	15	[	[	-LRB-
cord-014597-66vd2mdu	1185	16	3	3	CD
cord-014597-66vd2mdu	1185	17	]	]	-RRB-
cord-014597-66vd2mdu	1185	18	.	.	.
cord-014597-66vd2mdu	1186	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1186	2	,	,	,
cord-014597-66vd2mdu	1186	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1186	4	postulate	postulate	VBP
cord-014597-66vd2mdu	1186	5	that	that	IN
cord-014597-66vd2mdu	1186	6	the	the	DT
cord-014597-66vd2mdu	1186	7	SCS	SCS	NNP
cord-014597-66vd2mdu	1186	8	used	use	VBN
cord-014597-66vd2mdu	1186	9	for	for	IN
cord-014597-66vd2mdu	1186	10	encapsulation	encapsulation	NN
cord-014597-66vd2mdu	1186	11	has	have	VBZ
cord-014597-66vd2mdu	1186	12	biomimetic	biomimetic	JJ
cord-014597-66vd2mdu	1186	13	properties	property	NNS
cord-014597-66vd2mdu	1186	14	,	,	,
cord-014597-66vd2mdu	1186	15	creating	create	VBG
cord-014597-66vd2mdu	1186	16	an	an	DT
cord-014597-66vd2mdu	1186	17	artificial	artificial	JJ
cord-014597-66vd2mdu	1186	18	extracellular	extracellular	JJ
cord-014597-66vd2mdu	1186	19	matrix	matrix	NN
cord-014597-66vd2mdu	1186	20	mimicking	mimic	VBG
cord-014597-66vd2mdu	1186	21	heparin	heparin	NN
cord-014597-66vd2mdu	1186	22	sulfate	sulfate	NN
cord-014597-66vd2mdu	1186	23	which	which	WDT
cord-014597-66vd2mdu	1186	24	in	in	IN
cord-014597-66vd2mdu	1186	25	turn	turn	NN
cord-014597-66vd2mdu	1186	26	positively	positively	RB
cord-014597-66vd2mdu	1186	27	affect	affect	VBP
cord-014597-66vd2mdu	1186	28	T	T	NNP
cord-014597-66vd2mdu	1186	29	cell	cell	NN
cord-014597-66vd2mdu	1186	30	proliferation	proliferation	NN
cord-014597-66vd2mdu	1186	31	via	via	IN
cord-014597-66vd2mdu	1186	32	trans	trans	JJ
cord-014597-66vd2mdu	1186	33	-	-	NN
cord-014597-66vd2mdu	1186	34	presentation	presentation	NN
cord-014597-66vd2mdu	1186	35	of	of	IN
cord-014597-66vd2mdu	1186	36	IL-2	IL-2	NNP
cord-014597-66vd2mdu	1186	37	(	(	-LRB-
cord-014597-66vd2mdu	1186	38	Fig	Fig	NNP
cord-014597-66vd2mdu	1186	39	.	.	.
cord-014597-66vd2mdu	1186	40	1d	1d	CD
cord-014597-66vd2mdu	1186	41	)	)	-RRB-
cord-014597-66vd2mdu	1187	1	[	[	-LRB-
cord-014597-66vd2mdu	1187	2	4	4	CD
cord-014597-66vd2mdu	1187	3	]	]	-RRB-
cord-014597-66vd2mdu	1187	4	.	.	.
cord-014597-66vd2mdu	1188	1	Primary	primary	JJ
cord-014597-66vd2mdu	1188	2	T	t	NN
cord-014597-66vd2mdu	1188	3	lymphocytes	lymphocyte	NNS
cord-014597-66vd2mdu	1188	4	can	can	MD
cord-014597-66vd2mdu	1188	5	be	be	VB
cord-014597-66vd2mdu	1188	6	expanded	expand	VBN
cord-014597-66vd2mdu	1188	7	under	under	IN
cord-014597-66vd2mdu	1188	8	appropriate	appropriate	JJ
cord-014597-66vd2mdu	1188	9	conditions	condition	NNS
cord-014597-66vd2mdu	1188	10	outside	outside	IN
cord-014597-66vd2mdu	1188	11	the	the	DT
cord-014597-66vd2mdu	1188	12	body	body	NN
cord-014597-66vd2mdu	1188	13	.	.	.
cord-014597-66vd2mdu	1189	1	In	in	IN
cord-014597-66vd2mdu	1189	2	the	the	DT
cord-014597-66vd2mdu	1189	3	latter	latter	JJ
cord-014597-66vd2mdu	1189	4	,	,	,
cord-014597-66vd2mdu	1189	5	T	t	NN
cord-014597-66vd2mdu	1189	6	cells	cell	NNS
cord-014597-66vd2mdu	1189	7	grow	grow	VBP
cord-014597-66vd2mdu	1189	8	/	/	SYM
cord-014597-66vd2mdu	1189	9	expand	expand	VBP
cord-014597-66vd2mdu	1189	10	in	in	IN
cord-014597-66vd2mdu	1189	11	specific	specific	JJ
cord-014597-66vd2mdu	1189	12	environments	environment	NNS
cord-014597-66vd2mdu	1189	13	where	where	WRB
cord-014597-66vd2mdu	1189	14	the	the	DT
cord-014597-66vd2mdu	1189	15	cells	cell	NNS
cord-014597-66vd2mdu	1189	16	are	be	VBP
cord-014597-66vd2mdu	1189	17	tightly	tightly	RB
cord-014597-66vd2mdu	1189	18	packed	pack	VBN
cord-014597-66vd2mdu	1189	19	,	,	,
cord-014597-66vd2mdu	1189	20	leading	lead	VBG
cord-014597-66vd2mdu	1189	21	to	to	IN
cord-014597-66vd2mdu	1189	22	multiple	multiple	JJ
cord-014597-66vd2mdu	1189	23	cell	cell	NN
cord-014597-66vd2mdu	1189	24	-	-	HYPH
cord-014597-66vd2mdu	1189	25	cell	cell	NN
cord-014597-66vd2mdu	1189	26	contacts	contact	NNS
cord-014597-66vd2mdu	1189	27	and	and	CC
cord-014597-66vd2mdu	1189	28	manifold	manifold	JJ
cord-014597-66vd2mdu	1189	29	interactions	interaction	NNS
cord-014597-66vd2mdu	1189	30	with	with	IN
cord-014597-66vd2mdu	1189	31	the	the	DT
cord-014597-66vd2mdu	1189	32	extracellular	extracellular	JJ
cord-014597-66vd2mdu	1189	33	matrix	matrix	NN
cord-014597-66vd2mdu	1189	34	.	.	.
cord-014597-66vd2mdu	1190	1	Ex	Ex	NNP
cord-014597-66vd2mdu	1190	2	vivo	vivo	NN
cord-014597-66vd2mdu	1190	3	suspension	suspension	NN
cord-014597-66vd2mdu	1190	4	cultures	culture	NNS
cord-014597-66vd2mdu	1190	5	of	of	IN
cord-014597-66vd2mdu	1190	6	diluted	diluted	JJ
cord-014597-66vd2mdu	1190	7	cells	cell	NNS
cord-014597-66vd2mdu	1190	8	can	can	MD
cord-014597-66vd2mdu	1190	9	not	not	RB
cord-014597-66vd2mdu	1190	10	provide	provide	VB
cord-014597-66vd2mdu	1190	11	such	such	PDT
cord-014597-66vd2mdu	1190	12	a	a	DT
cord-014597-66vd2mdu	1190	13	microenvironment	microenvironment	NN
cord-014597-66vd2mdu	1190	14	.	.	.
cord-014597-66vd2mdu	1191	1	In	in	IN
cord-014597-66vd2mdu	1191	2	the	the	DT
cord-014597-66vd2mdu	1191	3	microcapsulesbased	microcapsulesbased	JJ
cord-014597-66vd2mdu	1191	4	cultivation	cultivation	NN
cord-014597-66vd2mdu	1191	5	system	system	NN
cord-014597-66vd2mdu	1191	6	presented	present	VBN
cord-014597-66vd2mdu	1191	7	,	,	,
cord-014597-66vd2mdu	1191	8	the	the	DT
cord-014597-66vd2mdu	1191	9	cells	cell	NNS
cord-014597-66vd2mdu	1191	10	are	be	VBP
cord-014597-66vd2mdu	1191	11	suspended	suspend	VBN
cord-014597-66vd2mdu	1191	12	in	in	IN
cord-014597-66vd2mdu	1191	13	a	a	DT
cord-014597-66vd2mdu	1191	14	viscous	viscous	JJ
cord-014597-66vd2mdu	1191	15	SCS	scs	NN
cord-014597-66vd2mdu	1191	16	-	-	HYPH
cord-014597-66vd2mdu	1191	17	solution	solution	NN
cord-014597-66vd2mdu	1191	18	.	.	.
cord-014597-66vd2mdu	1192	1	The	the	DT
cord-014597-66vd2mdu	1192	2	low	low	JJ
cord-014597-66vd2mdu	1192	3	molecular	molecular	JJ
cord-014597-66vd2mdu	1192	4	weight	weight	NN
cord-014597-66vd2mdu	1192	5	cut	cut	VBN
cord-014597-66vd2mdu	1192	6	off	off	IN
cord-014597-66vd2mdu	1192	7	of	of	IN
cord-014597-66vd2mdu	1192	8	the	the	DT
cord-014597-66vd2mdu	1192	9	surrounding	surround	VBG
cord-014597-66vd2mdu	1192	10	polyelectrolyte	polyelectrolyte	NNP
cord-014597-66vd2mdu	1192	11	membrane	membrane	NN
cord-014597-66vd2mdu	1192	12	assures	assure	VBZ
cord-014597-66vd2mdu	1192	13	that	that	IN
cord-014597-66vd2mdu	1192	14	typical	typical	JJ
cord-014597-66vd2mdu	1192	15	signaling	signal	VBG
cord-014597-66vd2mdu	1192	16	molecules	molecule	NNS
cord-014597-66vd2mdu	1192	17	produced	produce	VBN
cord-014597-66vd2mdu	1192	18	by	by	IN
cord-014597-66vd2mdu	1192	19	the	the	DT
cord-014597-66vd2mdu	1192	20	cells	cell	NNS
cord-014597-66vd2mdu	1192	21	are	be	VBP
cord-014597-66vd2mdu	1192	22	retained	retain	VBN
cord-014597-66vd2mdu	1192	23	thus	thus	RB
cord-014597-66vd2mdu	1192	24	facilitates	facilitate	VBZ
cord-014597-66vd2mdu	1192	25	the	the	DT
cord-014597-66vd2mdu	1192	26	"	"	``
cord-014597-66vd2mdu	1192	27	conditioning	conditioning	NN
cord-014597-66vd2mdu	1192	28	"	"	''
cord-014597-66vd2mdu	1192	29	of	of	IN
cord-014597-66vd2mdu	1192	30	the	the	DT
cord-014597-66vd2mdu	1192	31	cellular	cellular	JJ
cord-014597-66vd2mdu	1192	32	microenvironment	microenvironment	NN
cord-014597-66vd2mdu	1192	33	,	,	,
cord-014597-66vd2mdu	1192	34	while	while	IN
cord-014597-66vd2mdu	1192	35	nutrients	nutrient	NNS
cord-014597-66vd2mdu	1192	36	and	and	CC
cord-014597-66vd2mdu	1192	37	metabolites	metabolite	NNS
cord-014597-66vd2mdu	1192	38	can	can	MD
cord-014597-66vd2mdu	1192	39	pass	pass	VB
cord-014597-66vd2mdu	1192	40	.	.	.
cord-014597-66vd2mdu	1193	1	Expensive	expensive	JJ
cord-014597-66vd2mdu	1193	2	additives	additive	NNS
cord-014597-66vd2mdu	1193	3	,	,	,
cord-014597-66vd2mdu	1193	4	such	such	JJ
cord-014597-66vd2mdu	1193	5	as	as	IN
cord-014597-66vd2mdu	1193	6	interleukin-2	interleukin-2	IN
cord-014597-66vd2mdu	1193	7	(	(	-LRB-
cord-014597-66vd2mdu	1193	8	IL-2	IL-2	NNP
cord-014597-66vd2mdu	1193	9	)	)	-RRB-
cord-014597-66vd2mdu	1193	10	,	,	,
cord-014597-66vd2mdu	1193	11	can	can	MD
cord-014597-66vd2mdu	1193	12	be	be	VB
cord-014597-66vd2mdu	1193	13	co	co	VBN
cord-014597-66vd2mdu	1193	14	-	-	VBN
cord-014597-66vd2mdu	1193	15	encapsulated	encapsulate	VBN
cord-014597-66vd2mdu	1193	16	.	.	.
cord-014597-66vd2mdu	1194	1	Expansion	expansion	NN
cord-014597-66vd2mdu	1194	2	then	then	RB
cord-014597-66vd2mdu	1194	3	no	no	RB
cord-014597-66vd2mdu	1194	4	longer	longer	RB
cord-014597-66vd2mdu	1194	5	requires	require	VBZ
cord-014597-66vd2mdu	1194	6	specialized	specialized	JJ
cord-014597-66vd2mdu	1194	7	T	t	NN
cord-014597-66vd2mdu	1194	8	-	-	HYPH
cord-014597-66vd2mdu	1194	9	cell	cell	NN
cord-014597-66vd2mdu	1194	10	media	medium	NNS
cord-014597-66vd2mdu	1194	11	.	.	.
cord-014597-66vd2mdu	1195	1	Moreover	moreover	RB
cord-014597-66vd2mdu	1195	2	,	,	,
cord-014597-66vd2mdu	1195	3	the	the	DT
cord-014597-66vd2mdu	1195	4	SCS	SCS	NNP
cord-014597-66vd2mdu	1195	5	seems	seem	VBZ
cord-014597-66vd2mdu	1195	6	to	to	TO
cord-014597-66vd2mdu	1195	7	have	have	VB
cord-014597-66vd2mdu	1195	8	biomimetic	biomimetic	JJ
cord-014597-66vd2mdu	1195	9	properties	property	NNS
cord-014597-66vd2mdu	1195	10	,	,	,
cord-014597-66vd2mdu	1195	11	representing	represent	VBG
cord-014597-66vd2mdu	1195	12	an	an	DT
cord-014597-66vd2mdu	1195	13	artificial	artificial	JJ
cord-014597-66vd2mdu	1195	14	extracellular	extracellular	JJ
cord-014597-66vd2mdu	1195	15	matrix	matrix	NN
cord-014597-66vd2mdu	1195	16	mimicking	mimic	VBG
cord-014597-66vd2mdu	1195	17	heparin	heparin	NN
cord-014597-66vd2mdu	1195	18	sulfate	sulfate	NN
cord-014597-66vd2mdu	1195	19	.	.	.
cord-014597-66vd2mdu	1196	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1196	2	consider	consider	VBP
cord-014597-66vd2mdu	1196	3	that	that	IN
cord-014597-66vd2mdu	1196	4	the	the	DT
cord-014597-66vd2mdu	1196	5	described	describe	VBN
cord-014597-66vd2mdu	1196	6	method	method	NN
cord-014597-66vd2mdu	1196	7	may	may	MD
cord-014597-66vd2mdu	1196	8	be	be	VB
cord-014597-66vd2mdu	1196	9	an	an	DT
cord-014597-66vd2mdu	1196	10	appropriate	appropriate	JJ
cord-014597-66vd2mdu	1196	11	alternative	alternative	NN
cord-014597-66vd2mdu	1196	12	to	to	TO
cord-014597-66vd2mdu	1196	13	expand	expand	VB
cord-014597-66vd2mdu	1196	14	T	t	NN
cord-014597-66vd2mdu	1196	15	cells	cell	NNS
cord-014597-66vd2mdu	1196	16	while	while	IN
cord-014597-66vd2mdu	1196	17	creating	create	VBG
cord-014597-66vd2mdu	1196	18	a	a	DT
cord-014597-66vd2mdu	1196	19	local	local	JJ
cord-014597-66vd2mdu	1196	20	microenvironment	microenvironment	NN
cord-014597-66vd2mdu	1196	21	mimicking	mimic	VBG
cord-014597-66vd2mdu	1196	22	in	in	IN
cord-014597-66vd2mdu	1196	23	vivo	vivo	NN
cord-014597-66vd2mdu	1196	24	conditions	condition	NNS
cord-014597-66vd2mdu	1196	25	.	.	.
cord-014597-66vd2mdu	1197	1	-175	-175	NNP
cord-014597-66vd2mdu	1197	2	)	)	-RRB-
cord-014597-66vd2mdu	1197	3	.	.	.
cord-014597-66vd2mdu	1198	1	Equations	equation	NNS
cord-014597-66vd2mdu	1198	2	of	of	IN
cord-014597-66vd2mdu	1198	3	balances	balance	NNS
cord-014597-66vd2mdu	1198	4	and	and	CC
cord-014597-66vd2mdu	1198	5	kinetics	kinetic	NNS
cord-014597-66vd2mdu	1198	6	of	of	IN
cord-014597-66vd2mdu	1198	7	an	an	DT
cord-014597-66vd2mdu	1198	8	employed	employ	VBN
cord-014597-66vd2mdu	1198	9	process	process	NN
cord-014597-66vd2mdu	1198	10	model	model	NN
cord-014597-66vd2mdu	1198	11	including	include	VBG
cord-014597-66vd2mdu	1198	12	X	x	NN
cord-014597-66vd2mdu	1198	13	v	v	NN
cord-014597-66vd2mdu	1198	14	viable	viable	JJ
cord-014597-66vd2mdu	1198	15	cell	cell	NN
cord-014597-66vd2mdu	1198	16	density	density	NN
cord-014597-66vd2mdu	1198	17	,	,	,
cord-014597-66vd2mdu	1198	18	X	X	NNP
cord-014597-66vd2mdu	1198	19	t	t	NN
cord-014597-66vd2mdu	1198	20	total	total	JJ
cord-014597-66vd2mdu	1198	21	cell	cell	NN
cord-014597-66vd2mdu	1198	22	density	density	NN
cord-014597-66vd2mdu	1198	23	,	,	,
cord-014597-66vd2mdu	1198	24	μ	μ	NN
cord-014597-66vd2mdu	1198	25	cell	cell	NN
cord-014597-66vd2mdu	1198	26	-	-	HYPH
cord-014597-66vd2mdu	1198	27	specific	specific	JJ
cord-014597-66vd2mdu	1198	28	growth	growth	NN
cord-014597-66vd2mdu	1198	29	rate	rate	NN
cord-014597-66vd2mdu	1198	30	,	,	,
cord-014597-66vd2mdu	1199	1	μ	μ	NN
cord-014597-66vd2mdu	1199	2	d	d	NNP
cord-014597-66vd2mdu	1199	3	cell	cell	NN
cord-014597-66vd2mdu	1199	4	-	-	HYPH
cord-014597-66vd2mdu	1199	5	specific	specific	JJ
cord-014597-66vd2mdu	1199	6	death	death	NN
cord-014597-66vd2mdu	1199	7	rate	rate	NN
cord-014597-66vd2mdu	1199	8	,	,	,
cord-014597-66vd2mdu	1199	9	t	t	NN
cord-014597-66vd2mdu	1199	10	time	time	NN
cord-014597-66vd2mdu	1199	11	,	,	,
cord-014597-66vd2mdu	1199	12	K	K	NNP
cord-014597-66vd2mdu	1199	13	S	S	NNP
cord-014597-66vd2mdu	1199	14	and	and	CC
cord-014597-66vd2mdu	1199	15	k	k	NNP
cord-014597-66vd2mdu	1199	16	Monod	Monod	NNP
cord-014597-66vd2mdu	1199	17	kinetic	kinetic	JJ
cord-014597-66vd2mdu	1199	18	constant	constant	NN
cord-014597-66vd2mdu	1199	19	and	and	CC
cord-014597-66vd2mdu	1199	20	Monod	Monod	NNP
cord-014597-66vd2mdu	1199	21	constant	constant	JJ
cord-014597-66vd2mdu	1199	22	for	for	IN
cord-014597-66vd2mdu	1199	23	uptake	uptake	NN
cord-014597-66vd2mdu	1199	24	,	,	,
cord-014597-66vd2mdu	1199	25	K	K	NNP
cord-014597-66vd2mdu	1199	26	Lys	Lys	NNP
cord-014597-66vd2mdu	1199	27	cell	cell	NN
cord-014597-66vd2mdu	1199	28	lysis	lysis	NN
cord-014597-66vd2mdu	1199	29	constant	constant	JJ
cord-014597-66vd2mdu	1199	30	,	,	,
cord-014597-66vd2mdu	1199	31	q	q	NN
cord-014597-66vd2mdu	1199	32	cell	cell	NN
cord-014597-66vd2mdu	1199	33	-	-	HYPH
cord-014597-66vd2mdu	1199	34	specific	specific	JJ
cord-014597-66vd2mdu	1199	35	uptake	uptake	NN
cord-014597-66vd2mdu	1199	36	rate	rate	NN
cord-014597-66vd2mdu	1199	37	or	or	CC
cord-014597-66vd2mdu	1199	38	production	production	NN
cord-014597-66vd2mdu	1199	39	rate	rate	NN
cord-014597-66vd2mdu	1199	40	,	,	,
cord-014597-66vd2mdu	1199	41	respectively	respectively	RB
cord-014597-66vd2mdu	1199	42	,	,	,
cord-014597-66vd2mdu	1199	43	Y	Y	NNP
cord-014597-66vd2mdu	1199	44	kinetic	kinetic	JJ
cord-014597-66vd2mdu	1199	45	production	production	NN
cord-014597-66vd2mdu	1199	46	constant	constant	NN
cord-014597-66vd2mdu	1199	47	,	,	,
cord-014597-66vd2mdu	1199	48	c	c	NN
cord-014597-66vd2mdu	1199	49	concentration	concentration	NN
cord-014597-66vd2mdu	1199	50	,	,	,
cord-014597-66vd2mdu	1199	51	Glc	Glc	NNP
cord-014597-66vd2mdu	1199	52	glucose	glucose	NN
cord-014597-66vd2mdu	1199	53	,	,	,
cord-014597-66vd2mdu	1199	54	Gln	Gln	NNP
cord-014597-66vd2mdu	1199	55	glutamine	glutamine	NN
cord-014597-66vd2mdu	1199	56	,	,	,
cord-014597-66vd2mdu	1199	57	Lac	Lac	NNP
cord-014597-66vd2mdu	1199	58	lactate	lactate	NN
cord-014597-66vd2mdu	1199	59	,	,	,
cord-014597-66vd2mdu	1199	60	Amm	Amm	NNP
cord-014597-66vd2mdu	1199	61	ammonia	ammonia	NN
cord-014597-66vd2mdu	1199	62	,	,	,
cord-014597-66vd2mdu	1199	63	F	F	NNP
cord-014597-66vd2mdu	1199	64	feed	feed	NN
cord-014597-66vd2mdu	1199	65	rate	rate	NN
cord-014597-66vd2mdu	1199	66	,	,	,
cord-014597-66vd2mdu	1199	67	V	v	NN
cord-014597-66vd2mdu	1199	68	volume	volume	NN
cord-014597-66vd2mdu	1199	69	Balances	balance	NNS
cord-014597-66vd2mdu	1199	70	with	with	IN
cord-014597-66vd2mdu	1199	71	fed	fed	NNP
cord-014597-66vd2mdu	1199	72	-	-	HYPH
cord-014597-66vd2mdu	1199	73	batch	batch	NN
cord-014597-66vd2mdu	1199	74	terms	term	NNS
cord-014597-66vd2mdu	1199	75	Kinetics	kinetic	NNS
cord-014597-66vd2mdu	1199	76	;	;	:
cord-014597-66vd2mdu	1199	77	uptake	uptake	NN
cord-014597-66vd2mdu	1199	78	if	if	IN
cord-014597-66vd2mdu	1199	79	c	c	NNP
cord-014597-66vd2mdu	1199	80	Glc	Glc	NNP
cord-014597-66vd2mdu	1199	81	≥	≥	CD
cord-014597-66vd2mdu	1199	82	0.5	0.5	CD
cord-014597-66vd2mdu	1199	83	mM	mm	NN
cord-014597-66vd2mdu	1199	84	:	:	:
cord-014597-66vd2mdu	1199	85	q	q	NNP
cord-014597-66vd2mdu	1199	86	Lac	Lac	NNP
cord-014597-66vd2mdu	1199	87	,	,	,
cord-014597-66vd2mdu	1199	88	uptake	uptake	NN
cord-014597-66vd2mdu	1199	89	=	=	SYM
cord-014597-66vd2mdu	1199	90	0	0	NFP
cord-014597-66vd2mdu	1200	1	if	if	IN
cord-014597-66vd2mdu	1200	2	c	c	NNP
cord-014597-66vd2mdu	1200	3	Glc	Glc	NNP
cord-014597-66vd2mdu	1200	4	<	<	XX
cord-014597-66vd2mdu	1200	5	0.5	0.5	CD
cord-014597-66vd2mdu	1200	6	mM	mm	CD
cord-014597-66vd2mdu	1200	7	:	:	:
cord-014597-66vd2mdu	1200	8	q	q	NNP
cord-014597-66vd2mdu	1200	9	Lac	Lac	NNP
cord-014597-66vd2mdu	1200	10	,	,	,
cord-014597-66vd2mdu	1200	11	uptake	uptake	NN
cord-014597-66vd2mdu	1200	12	=	=	NN
cord-014597-66vd2mdu	1200	13	q	q	NNP
cord-014597-66vd2mdu	1200	14	Lac	Lac	NNP
cord-014597-66vd2mdu	1200	15	,	,	,
cord-014597-66vd2mdu	1200	16	uptake	uptake	NN
cord-014597-66vd2mdu	1200	17	,	,	,
cord-014597-66vd2mdu	1200	18	max	max	NNP
cord-014597-66vd2mdu	1200	19	q	q	NNP
cord-014597-66vd2mdu	1201	1	Amm	Amm	NNP
cord-014597-66vd2mdu	1201	2	=	=	NNP
cord-014597-66vd2mdu	1201	3	Y	y	NN
cord-014597-66vd2mdu	1202	1	Amm	Amm	NNP
cord-014597-66vd2mdu	1202	2	/	/	SYM
cord-014597-66vd2mdu	1202	3	Gln	Gln	NNP
cord-014597-66vd2mdu	1202	4	•	•	NNP
cord-014597-66vd2mdu	1203	1	q	q	NNP
cord-014597-66vd2mdu	1203	2	Gln	Gln	NNP
cord-014597-66vd2mdu	1203	3	Background	Background	NNP
cord-014597-66vd2mdu	1203	4	Digital	Digital	NNP
cord-014597-66vd2mdu	1203	5	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1203	6	(	(	-LRB-
cord-014597-66vd2mdu	1203	7	DM	DM	NNP
cord-014597-66vd2mdu	1203	8	)	)	-RRB-
cord-014597-66vd2mdu	1203	9	is	be	VBZ
cord-014597-66vd2mdu	1203	10	heightening	heighten	VBG
cord-014597-66vd2mdu	1203	11	the	the	DT
cord-014597-66vd2mdu	1203	12	productivity	productivity	NN
cord-014597-66vd2mdu	1203	13	and	and	CC
cord-014597-66vd2mdu	1203	14	robustness	robustness	NN
cord-014597-66vd2mdu	1203	15	of	of	IN
cord-014597-66vd2mdu	1203	16	existing	exist	VBG
cord-014597-66vd2mdu	1203	17	processes	process	NNS
cord-014597-66vd2mdu	1203	18	and	and	CC
cord-014597-66vd2mdu	1203	19	facilities	facility	NNS
cord-014597-66vd2mdu	1203	20	.	.	.
cord-014597-66vd2mdu	1204	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1204	2	also	also	RB
cord-014597-66vd2mdu	1204	3	enables	enable	VBZ
cord-014597-66vd2mdu	1204	4	the	the	DT
cord-014597-66vd2mdu	1204	5	efficient	efficient	JJ
cord-014597-66vd2mdu	1204	6	development	development	NN
cord-014597-66vd2mdu	1204	7	of	of	IN
cord-014597-66vd2mdu	1204	8	previously	previously	RB
cord-014597-66vd2mdu	1204	9	unmanageable	unmanageable	JJ
cord-014597-66vd2mdu	1204	10	products	product	NNS
cord-014597-66vd2mdu	1204	11	or	or	CC
cord-014597-66vd2mdu	1204	12	processes	process	NNS
cord-014597-66vd2mdu	1204	13	and	and	CC
cord-014597-66vd2mdu	1204	14	provided	provide	VBD
cord-014597-66vd2mdu	1204	15	the	the	DT
cord-014597-66vd2mdu	1204	16	basis	basis	NN
cord-014597-66vd2mdu	1204	17	for	for	IN
cord-014597-66vd2mdu	1204	18	a	a	DT
cord-014597-66vd2mdu	1204	19	wave	wave	NN
cord-014597-66vd2mdu	1204	20	of	of	IN
cord-014597-66vd2mdu	1204	21	innovations	innovation	NNS
cord-014597-66vd2mdu	1204	22	.	.	.
cord-014597-66vd2mdu	1205	1	DM	DM	NNP
cord-014597-66vd2mdu	1205	2	is	be	VBZ
cord-014597-66vd2mdu	1205	3	a	a	DT
cord-014597-66vd2mdu	1205	4	resident	resident	NN
cord-014597-66vd2mdu	1205	5	and	and	CC
cord-014597-66vd2mdu	1205	6	on	on	IN
cord-014597-66vd2mdu	1205	7	-	-	HYPH
cord-014597-66vd2mdu	1205	8	line	line	NN
cord-014597-66vd2mdu	1205	9	source	source	NN
cord-014597-66vd2mdu	1205	10	of	of	IN
cord-014597-66vd2mdu	1205	11	continuous	continuous	JJ
cord-014597-66vd2mdu	1205	12	optimization	optimization	NN
cord-014597-66vd2mdu	1205	13	of	of	IN
cord-014597-66vd2mdu	1205	14	process	process	NN
cord-014597-66vd2mdu	1205	15	performance	performance	NN
cord-014597-66vd2mdu	1205	16	.	.	.
cord-014597-66vd2mdu	1206	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1206	2	relies	rely	VBZ
cord-014597-66vd2mdu	1206	3	upon	upon	IN
cord-014597-66vd2mdu	1206	4	the	the	DT
cord-014597-66vd2mdu	1206	5	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	1206	6	,	,	,
cord-014597-66vd2mdu	1206	7	real	real	JJ
cord-014597-66vd2mdu	1206	8	-	-	HYPH
cord-014597-66vd2mdu	1206	9	time	time	NN
cord-014597-66vd2mdu	1206	10	interfacing	interfacing	NN
cord-014597-66vd2mdu	1206	11	of	of	IN
cord-014597-66vd2mdu	1206	12	both	both	DT
cord-014597-66vd2mdu	1206	13	human	human	JJ
cord-014597-66vd2mdu	1206	14	and	and	CC
cord-014597-66vd2mdu	1206	15	machine	machine	NN
cord-014597-66vd2mdu	1206	16	sourced	source	VBD
cord-014597-66vd2mdu	1206	17	information	information	NN
cord-014597-66vd2mdu	1206	18	through	through	IN
cord-014597-66vd2mdu	1206	19	one	one	CD
cord-014597-66vd2mdu	1206	20	centralized	centralized	JJ
cord-014597-66vd2mdu	1206	21	system	system	NN
cord-014597-66vd2mdu	1206	22	.	.	.
cord-014597-66vd2mdu	1207	1	More	More	JJR
cord-014597-66vd2mdu	1207	2	than	than	IN
cord-014597-66vd2mdu	1207	3	legacy	legacy	NN
cord-014597-66vd2mdu	1207	4	Distributed	Distributed	NNP
cord-014597-66vd2mdu	1207	5	Control	Control	NNP
cord-014597-66vd2mdu	1207	6	System	System	NNP
cord-014597-66vd2mdu	1207	7	(	(	-LRB-
cord-014597-66vd2mdu	1207	8	DCS	DCS	NNP
cord-014597-66vd2mdu	1207	9	)	)	-RRB-
cord-014597-66vd2mdu	1207	10	and	and	CC
cord-014597-66vd2mdu	1207	11	supervisory	supervisory	JJ
cord-014597-66vd2mdu	1207	12	control	control	NN
cord-014597-66vd2mdu	1207	13	and	and	CC
cord-014597-66vd2mdu	1207	14	data	datum	NNS
cord-014597-66vd2mdu	1207	15	acquisition	acquisition	NN
cord-014597-66vd2mdu	1207	16	(	(	-LRB-
cord-014597-66vd2mdu	1207	17	SCADA	SCADA	NNP
cord-014597-66vd2mdu	1207	18	)	)	-RRB-
cord-014597-66vd2mdu	1207	19	,	,	,
cord-014597-66vd2mdu	1207	20	it	-PRON-	PRP
cord-014597-66vd2mdu	1207	21	is	be	VBZ
cord-014597-66vd2mdu	1207	22	an	an	DT
cord-014597-66vd2mdu	1207	23	integral	integral	JJ
cord-014597-66vd2mdu	1207	24	interconnection	interconnection	NN
cord-014597-66vd2mdu	1207	25	of	of	IN
cord-014597-66vd2mdu	1207	26	real	real	JJ
cord-014597-66vd2mdu	1207	27	-	-	HYPH
cord-014597-66vd2mdu	1207	28	time	time	NN
cord-014597-66vd2mdu	1207	29	access	access	NN
cord-014597-66vd2mdu	1207	30	to	to	IN
cord-014597-66vd2mdu	1207	31	divergent	divergent	JJ
cord-014597-66vd2mdu	1207	32	sources	source	NNS
cord-014597-66vd2mdu	1207	33	of	of	IN
cord-014597-66vd2mdu	1207	34	information	information	NN
cord-014597-66vd2mdu	1207	35	.	.	.
cord-014597-66vd2mdu	1208	1	As	as	IN
cord-014597-66vd2mdu	1208	2	such	such	JJ
cord-014597-66vd2mdu	1208	3	,	,	,
cord-014597-66vd2mdu	1208	4	it	-PRON-	PRP
cord-014597-66vd2mdu	1208	5	can	can	MD
cord-014597-66vd2mdu	1208	6	promise	promise	VB
cord-014597-66vd2mdu	1208	7	deep	deep	JJ
cord-014597-66vd2mdu	1208	8	analysis	analysis	NN
cord-014597-66vd2mdu	1208	9	and	and	CC
cord-014597-66vd2mdu	1208	10	predictions	prediction	NNS
cord-014597-66vd2mdu	1208	11	leading	lead	VBG
cord-014597-66vd2mdu	1208	12	to	to	IN
cord-014597-66vd2mdu	1208	13	shortened	shorten	VBN
cord-014597-66vd2mdu	1208	14	product	product	NN
cord-014597-66vd2mdu	1208	15	cycle	cycle	NN
cord-014597-66vd2mdu	1208	16	and	and	CC
cord-014597-66vd2mdu	1208	17	advanced	advanced	JJ
cord-014597-66vd2mdu	1208	18	process	process	NN
cord-014597-66vd2mdu	1208	19	control	control	NN
cord-014597-66vd2mdu	1208	20	.	.	.
cord-014597-66vd2mdu	1209	1	This	this	DT
cord-014597-66vd2mdu	1209	2	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	1209	3	analysis	analysis	NN
cord-014597-66vd2mdu	1209	4	is	be	VBZ
cord-014597-66vd2mdu	1209	5	extending	extend	VBG
cord-014597-66vd2mdu	1209	6	beyond	beyond	IN
cord-014597-66vd2mdu	1209	7	operations	operation	NNS
cord-014597-66vd2mdu	1209	8	performance	performance	NN
cord-014597-66vd2mdu	1209	9	data	datum	NNS
cord-014597-66vd2mdu	1209	10	from	from	IN
cord-014597-66vd2mdu	1209	11	the	the	DT
cord-014597-66vd2mdu	1209	12	production	production	NN
cord-014597-66vd2mdu	1209	13	floor	floor	NN
cord-014597-66vd2mdu	1209	14	to	to	IN
cord-014597-66vd2mdu	1209	15	data	datum	NNS
cord-014597-66vd2mdu	1209	16	driving	drive	VBG
cord-014597-66vd2mdu	1209	17	such	such	JJ
cord-014597-66vd2mdu	1209	18	activities	activity	NNS
cord-014597-66vd2mdu	1209	19	as	as	IN
cord-014597-66vd2mdu	1209	20	raw	raw	JJ
cord-014597-66vd2mdu	1209	21	materials	material	NNS
cord-014597-66vd2mdu	1209	22	security	security	NN
cord-014597-66vd2mdu	1209	23	of	of	IN
cord-014597-66vd2mdu	1209	24	supply	supply	NN
cord-014597-66vd2mdu	1209	25	(	(	-LRB-
cord-014597-66vd2mdu	1209	26	SoS	SoS	NNP
cord-014597-66vd2mdu	1209	27	)	)	-RRB-
cord-014597-66vd2mdu	1209	28	and	and	CC
cord-014597-66vd2mdu	1209	29	business	business	NN
cord-014597-66vd2mdu	1209	30	continuity	continuity	NN
cord-014597-66vd2mdu	1209	31	management	management	NN
cord-014597-66vd2mdu	1209	32	systems	system	NNS
cord-014597-66vd2mdu	1209	33	(	(	-LRB-
cord-014597-66vd2mdu	1209	34	BCMS	BCMS	NNP
cord-014597-66vd2mdu	1209	35	)	)	-RRB-
cord-014597-66vd2mdu	1209	36	.	.	.
cord-014597-66vd2mdu	1210	1	Digital	Digital	NNP
cord-014597-66vd2mdu	1210	2	Biomanufacturing	Biomanufacturing	NNP
cord-014597-66vd2mdu	1210	3	(	(	-LRB-
cord-014597-66vd2mdu	1210	4	DB	DB	NNP
cord-014597-66vd2mdu	1210	5	)	)	-RRB-
cord-014597-66vd2mdu	1210	6	can	can	MD
cord-014597-66vd2mdu	1210	7	be	be	VB
cord-014597-66vd2mdu	1210	8	viewed	view	VBN
cord-014597-66vd2mdu	1210	9	as	as	RB
cord-014597-66vd2mdu	1210	10	yet	yet	RB
cord-014597-66vd2mdu	1210	11	another	another	DT
cord-014597-66vd2mdu	1210	12	,	,	,
cord-014597-66vd2mdu	1210	13	larger	large	JJR
cord-014597-66vd2mdu	1210	14	,	,	,
cord-014597-66vd2mdu	1210	15	embodiment	embodiment	NN
cord-014597-66vd2mdu	1210	16	of	of	IN
cord-014597-66vd2mdu	1210	17	digital	digital	JJ
cord-014597-66vd2mdu	1210	18	biotechnology	biotechnology	NN
cord-014597-66vd2mdu	1210	19	.	.	.
cord-014597-66vd2mdu	1211	1	DB	DB	NNP
cord-014597-66vd2mdu	1211	2	is	be	VBZ
cord-014597-66vd2mdu	1211	3	similar	similar	JJ
cord-014597-66vd2mdu	1211	4	to	to	IN
cord-014597-66vd2mdu	1211	5	digital	digital	JJ
cord-014597-66vd2mdu	1211	6	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1211	7	in	in	IN
cord-014597-66vd2mdu	1211	8	that	that	IN
cord-014597-66vd2mdu	1211	9	it	-PRON-	PRP
cord-014597-66vd2mdu	1211	10	promotes	promote	VBZ
cord-014597-66vd2mdu	1211	11	innovations	innovation	NNS
cord-014597-66vd2mdu	1211	12	in	in	IN
cord-014597-66vd2mdu	1211	13	the	the	DT
cord-014597-66vd2mdu	1211	14	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1211	15	of	of	IN
cord-014597-66vd2mdu	1211	16	biologicals	biological	NNS
cord-014597-66vd2mdu	1211	17	by	by	IN
cord-014597-66vd2mdu	1211	18	using	use	VBG
cord-014597-66vd2mdu	1211	19	such	such	JJ
cord-014597-66vd2mdu	1211	20	things	thing	NNS
cord-014597-66vd2mdu	1211	21	as	as	IN
cord-014597-66vd2mdu	1211	22	computer	computer	NN
cord-014597-66vd2mdu	1211	23	aided	aid	VBD
cord-014597-66vd2mdu	1211	24	design	design	NN
cord-014597-66vd2mdu	1211	25	,	,	,
cord-014597-66vd2mdu	1211	26	manufacture	manufacture	NN
cord-014597-66vd2mdu	1211	27	,	,	,
cord-014597-66vd2mdu	1211	28	verification	verification	NN
cord-014597-66vd2mdu	1211	29	and	and	CC
cord-014597-66vd2mdu	1211	30	deep	deep	JJ
cord-014597-66vd2mdu	1211	31	process	process	NN
cord-014597-66vd2mdu	1211	32	analysis	analysis	NN
cord-014597-66vd2mdu	1211	33	using	use	VBG
cord-014597-66vd2mdu	1211	34	software	software	NN
cord-014597-66vd2mdu	1211	35	sensors	sensor	NNS
cord-014597-66vd2mdu	1211	36	(	(	-LRB-
cord-014597-66vd2mdu	1211	37	Fig	Fig	NNP
cord-014597-66vd2mdu	1211	38	.	.	.
cord-014597-66vd2mdu	1211	39	1	1	CD
cord-014597-66vd2mdu	1211	40	)	)	-RRB-
cord-014597-66vd2mdu	1211	41	.	.	.
cord-014597-66vd2mdu	1212	1	However	however	RB
cord-014597-66vd2mdu	1212	2	,	,	,
cord-014597-66vd2mdu	1212	3	the	the	DT
cord-014597-66vd2mdu	1212	4	fact	fact	NN
cord-014597-66vd2mdu	1212	5	that	that	IN
cord-014597-66vd2mdu	1212	6	there	there	EX
cord-014597-66vd2mdu	1212	7	are	be	VBP
cord-014597-66vd2mdu	1212	8	living	live	VBG
cord-014597-66vd2mdu	1212	9	components	component	NNS
cord-014597-66vd2mdu	1212	10	(	(	-LRB-
cord-014597-66vd2mdu	1212	11	cells	cell	NNS
cord-014597-66vd2mdu	1212	12	)	)	-RRB-
cord-014597-66vd2mdu	1212	13	involved	involve	VBN
cord-014597-66vd2mdu	1212	14	in	in	IN
cord-014597-66vd2mdu	1212	15	the	the	DT
cord-014597-66vd2mdu	1212	16	processes	process	NNS
cord-014597-66vd2mdu	1212	17	puts	put	VBZ
cord-014597-66vd2mdu	1212	18	a	a	DT
cord-014597-66vd2mdu	1212	19	distinctly	distinctly	RB
cord-014597-66vd2mdu	1212	20	different	different	JJ
cord-014597-66vd2mdu	1212	21	flavor	flavor	NN
cord-014597-66vd2mdu	1212	22	to	to	IN
cord-014597-66vd2mdu	1212	23	the	the	DT
cord-014597-66vd2mdu	1212	24	systems	system	NNS
cord-014597-66vd2mdu	1212	25	employed	employ	VBN
cord-014597-66vd2mdu	1212	26	.	.	.
cord-014597-66vd2mdu	1213	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1213	2	is	be	VBZ
cord-014597-66vd2mdu	1213	3	desirable	desirable	JJ
cord-014597-66vd2mdu	1213	4	to	to	TO
cord-014597-66vd2mdu	1213	5	use	use	VB
cord-014597-66vd2mdu	1213	6	a	a	DT
cord-014597-66vd2mdu	1213	7	distinct	distinct	JJ
cord-014597-66vd2mdu	1213	8	term	term	NN
cord-014597-66vd2mdu	1213	9	here	here	RB
cord-014597-66vd2mdu	1213	10	to	to	TO
cord-014597-66vd2mdu	1213	11	distinguish	distinguish	VB
cord-014597-66vd2mdu	1213	12	it	-PRON-	PRP
cord-014597-66vd2mdu	1213	13	because	because	IN
cord-014597-66vd2mdu	1213	14	,	,	,
cord-014597-66vd2mdu	1213	15	as	as	IN
cord-014597-66vd2mdu	1213	16	in	in	IN
cord-014597-66vd2mdu	1213	17	the	the	DT
cord-014597-66vd2mdu	1213	18	terms	term	NNS
cord-014597-66vd2mdu	1213	19	bioproduction	bioproduction	NN
cord-014597-66vd2mdu	1213	20	and	and	CC
cord-014597-66vd2mdu	1213	21	biopharmacology	biopharmacology	NN
cord-014597-66vd2mdu	1213	22	,	,	,
cord-014597-66vd2mdu	1213	23	DB	DB	NNP
cord-014597-66vd2mdu	1213	24	addresses	address	VBZ
cord-014597-66vd2mdu	1213	25	many	many	JJ
cord-014597-66vd2mdu	1213	26	unique	unique	JJ
cord-014597-66vd2mdu	1213	27	aspects	aspect	NNS
cord-014597-66vd2mdu	1213	28	of	of	IN
cord-014597-66vd2mdu	1213	29	biologically	biologically	RB
cord-014597-66vd2mdu	1213	30	-	-	HYPH
cord-014597-66vd2mdu	1213	31	based	base	VBN
cord-014597-66vd2mdu	1213	32	activities	activity	NNS
cord-014597-66vd2mdu	1213	33	.	.	.
cord-014597-66vd2mdu	1214	1	The	the	DT
cord-014597-66vd2mdu	1214	2	reasons	reason	NNS
cord-014597-66vd2mdu	1214	3	why	why	WRB
cord-014597-66vd2mdu	1214	4	the	the	DT
cord-014597-66vd2mdu	1214	5	biotech	biotech	NN
cord-014597-66vd2mdu	1214	6	and	and	CC
cord-014597-66vd2mdu	1214	7	biopharma	biopharma	NN
cord-014597-66vd2mdu	1214	8	industry	industry	NN
cord-014597-66vd2mdu	1214	9	lags	lag	VBZ
cord-014597-66vd2mdu	1214	10	behind	behind	IN
cord-014597-66vd2mdu	1214	11	other	other	JJ
cord-014597-66vd2mdu	1214	12	sectors	sector	NNS
cord-014597-66vd2mdu	1214	13	such	such	JJ
cord-014597-66vd2mdu	1214	14	as	as	IN
cord-014597-66vd2mdu	1214	15	the	the	DT
cord-014597-66vd2mdu	1214	16	automotive	automotive	NN
cord-014597-66vd2mdu	1214	17	regarding	regard	VBG
cord-014597-66vd2mdu	1214	18	the	the	DT
cord-014597-66vd2mdu	1214	19	transformation	transformation	NN
cord-014597-66vd2mdu	1214	20	to	to	IN
cord-014597-66vd2mdu	1214	21	digital	digital	JJ
cord-014597-66vd2mdu	1214	22	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1214	23	are	be	VBP
cord-014597-66vd2mdu	1214	24	(	(	-LRB-
cord-014597-66vd2mdu	1214	25	i	i	NN
cord-014597-66vd2mdu	1214	26	)	)	-RRB-
cord-014597-66vd2mdu	1215	1	the	the	DT
cord-014597-66vd2mdu	1215	2	complexity	complexity	NN
cord-014597-66vd2mdu	1215	3	and	and	CC
cord-014597-66vd2mdu	1215	4	dissipative	dissipative	JJ
cord-014597-66vd2mdu	1215	5	nature	nature	NN
cord-014597-66vd2mdu	1215	6	of	of	IN
cord-014597-66vd2mdu	1215	7	biological	biological	JJ
cord-014597-66vd2mdu	1215	8	systems	system	NNS
cord-014597-66vd2mdu	1215	9	,	,	,
cord-014597-66vd2mdu	1215	10	(	(	-LRB-
cord-014597-66vd2mdu	1215	11	ii	ii	NN
cord-014597-66vd2mdu	1215	12	)	)	-RRB-
cord-014597-66vd2mdu	1215	13	distributed	distribute	VBD
cord-014597-66vd2mdu	1215	14	heterogeneous	heterogeneous	JJ
cord-014597-66vd2mdu	1215	15	data	datum	NNS
cord-014597-66vd2mdu	1215	16	and	and	CC
cord-014597-66vd2mdu	1215	17	(	(	-LRB-
cord-014597-66vd2mdu	1215	18	iii	iii	NN
cord-014597-66vd2mdu	1215	19	)	)	-RRB-
cord-014597-66vd2mdu	1215	20	limited	limit	VBN
cord-014597-66vd2mdu	1215	21	at	at	IN
cord-014597-66vd2mdu	1215	22	-	-	HYPH
cord-014597-66vd2mdu	1215	23	line	line	NN
cord-014597-66vd2mdu	1215	24	or	or	CC
cord-014597-66vd2mdu	1215	25	on	on	IN
cord-014597-66vd2mdu	1215	26	-	-	HYPH
cord-014597-66vd2mdu	1215	27	line	line	NN
cord-014597-66vd2mdu	1215	28	data	datum	NNS
cord-014597-66vd2mdu	1215	29	sources	source	NNS
cord-014597-66vd2mdu	1215	30	.	.	.
cord-014597-66vd2mdu	1216	1	However	however	RB
cord-014597-66vd2mdu	1216	2	,	,	,
cord-014597-66vd2mdu	1216	3	the	the	DT
cord-014597-66vd2mdu	1216	4	costs	cost	NNS
cord-014597-66vd2mdu	1216	5	of	of	IN
cord-014597-66vd2mdu	1216	6	genomic	genomic	JJ
cord-014597-66vd2mdu	1216	7	sequencing	sequencing	NN
cord-014597-66vd2mdu	1216	8	,	,	,
cord-014597-66vd2mdu	1216	9	omics	omics	NNP
cord-014597-66vd2mdu	1216	10	data	data	NN
cord-014597-66vd2mdu	1216	11	generation	generation	NN
cord-014597-66vd2mdu	1216	12	,	,	,
cord-014597-66vd2mdu	1216	13	and	and	CC
cord-014597-66vd2mdu	1216	14	computing	computing	NN
cord-014597-66vd2mdu	1216	15	resources	resource	NNS
cord-014597-66vd2mdu	1216	16	are	be	VBP
cord-014597-66vd2mdu	1216	17	decreasing	decrease	VBG
cord-014597-66vd2mdu	1216	18	rapidly	rapidly	RB
cord-014597-66vd2mdu	1216	19	,	,	,
cord-014597-66vd2mdu	1216	20	and	and	CC
cord-014597-66vd2mdu	1216	21	at	at	IN
cord-014597-66vd2mdu	1216	22	the	the	DT
cord-014597-66vd2mdu	1216	23	same	same	JJ
cord-014597-66vd2mdu	1216	24	time	time	NN
cord-014597-66vd2mdu	1216	25	process	process	NN
cord-014597-66vd2mdu	1216	26	analytical	analytical	JJ
cord-014597-66vd2mdu	1216	27	technologies	technology	NNS
cord-014597-66vd2mdu	1216	28	,	,	,
cord-014597-66vd2mdu	1216	29	computational	computational	JJ
cord-014597-66vd2mdu	1216	30	power	power	NN
cord-014597-66vd2mdu	1216	31	and	and	CC
cord-014597-66vd2mdu	1216	32	predictive	predictive	JJ
cord-014597-66vd2mdu	1216	33	modeling	modeling	NN
cord-014597-66vd2mdu	1216	34	as	as	RB
cord-014597-66vd2mdu	1216	35	well	well	RB
cord-014597-66vd2mdu	1216	36	as	as	IN
cord-014597-66vd2mdu	1216	37	data	datum	NNS
cord-014597-66vd2mdu	1216	38	management	management	NN
cord-014597-66vd2mdu	1216	39	infrastructures	infrastructure	NNS
cord-014597-66vd2mdu	1216	40	are	be	VBP
cord-014597-66vd2mdu	1216	41	greatly	greatly	RB
cord-014597-66vd2mdu	1216	42	improving	improve	VBG
cord-014597-66vd2mdu	1216	43	(	(	-LRB-
cord-014597-66vd2mdu	1216	44	Table	table	NN
cord-014597-66vd2mdu	1216	45	1	1	CD
cord-014597-66vd2mdu	1216	46	)	)	-RRB-
cord-014597-66vd2mdu	1216	47	.	.	.
cord-014597-66vd2mdu	1217	1	By	by	IN
cord-014597-66vd2mdu	1217	2	removing	remove	VBG
cord-014597-66vd2mdu	1217	3	roadblocks	roadblock	NNS
cord-014597-66vd2mdu	1217	4	that	that	WDT
cord-014597-66vd2mdu	1217	5	used	use	VBD
cord-014597-66vd2mdu	1217	6	to	to	TO
cord-014597-66vd2mdu	1217	7	limit	limit	VB
cord-014597-66vd2mdu	1217	8	approaches	approach	NNS
cord-014597-66vd2mdu	1217	9	,	,	,
cord-014597-66vd2mdu	1217	10	these	these	DT
cord-014597-66vd2mdu	1217	11	changes	change	NNS
cord-014597-66vd2mdu	1217	12	have	have	VBP
cord-014597-66vd2mdu	1217	13	paved	pave	VBN
cord-014597-66vd2mdu	1217	14	the	the	DT
cord-014597-66vd2mdu	1217	15	way	way	NN
cord-014597-66vd2mdu	1217	16	to	to	IN
cord-014597-66vd2mdu	1217	17	transforming	transform	VBG
cord-014597-66vd2mdu	1217	18	the	the	DT
cord-014597-66vd2mdu	1217	19	bioeconomy	bioeconomy	NN
cord-014597-66vd2mdu	1217	20	into	into	IN
cord-014597-66vd2mdu	1217	21	an	an	DT
cord-014597-66vd2mdu	1217	22	industry	industry	NN
cord-014597-66vd2mdu	1217	23	that	that	WDT
cord-014597-66vd2mdu	1217	24	is	be	VBZ
cord-014597-66vd2mdu	1217	25	based	base	VBN
cord-014597-66vd2mdu	1217	26	on	on	IN
cord-014597-66vd2mdu	1217	27	digital	digital	JJ
cord-014597-66vd2mdu	1217	28	knowledge	knowledge	NN
cord-014597-66vd2mdu	1217	29	.	.	.
cord-014597-66vd2mdu	1218	1	Such	such	JJ
cord-014597-66vd2mdu	1218	2	new	new	JJ
cord-014597-66vd2mdu	1218	3	and	and	CC
cord-014597-66vd2mdu	1218	4	optimized	optimize	VBN
cord-014597-66vd2mdu	1218	5	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1218	6	technologies	technology	NNS
cord-014597-66vd2mdu	1218	7	as	as	IN
cord-014597-66vd2mdu	1218	8	continuous	continuous	JJ
cord-014597-66vd2mdu	1218	9	biomanufacturing	biomanufacturing	NN
cord-014597-66vd2mdu	1218	10	and	and	CC
cord-014597-66vd2mdu	1218	11	3D	3D	NNP
cord-014597-66vd2mdu	1218	12	bioprinting	bioprinting	NN
cord-014597-66vd2mdu	1218	13	can	can	MD
cord-014597-66vd2mdu	1218	14	actually	actually	RB
cord-014597-66vd2mdu	1218	15	demand	demand	VB
cord-014597-66vd2mdu	1218	16	the	the	DT
cord-014597-66vd2mdu	1218	17	interfacing	interfacing	NN
cord-014597-66vd2mdu	1218	18	of	of	IN
cord-014597-66vd2mdu	1218	19	many	many	JJ
cord-014597-66vd2mdu	1218	20	sources	source	NNS
cord-014597-66vd2mdu	1218	21	of	of	IN
cord-014597-66vd2mdu	1218	22	information	information	NN
cord-014597-66vd2mdu	1218	23	,	,	,
cord-014597-66vd2mdu	1218	24	deep	deep	JJ
cord-014597-66vd2mdu	1218	25	data	datum	NNS
cord-014597-66vd2mdu	1218	26	Prior	prior	RB
cord-014597-66vd2mdu	1218	27	to	to	IN
cord-014597-66vd2mdu	1218	28	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1218	29	,	,	,
cord-014597-66vd2mdu	1218	30	the	the	DT
cord-014597-66vd2mdu	1218	31	various	various	JJ
cord-014597-66vd2mdu	1218	32	proteins	protein	NNS
cord-014597-66vd2mdu	1218	33	were	be	VBD
cord-014597-66vd2mdu	1218	34	incubated	incubate	VBN
cord-014597-66vd2mdu	1218	35	at	at	IN
cord-014597-66vd2mdu	1218	36	37	37	CD
cord-014597-66vd2mdu	1218	37	°	°	NN
cord-014597-66vd2mdu	1218	38	C	c	NN
cord-014597-66vd2mdu	1218	39	in	in	IN
cord-014597-66vd2mdu	1218	40	SCS	SCS	NNP
cord-014597-66vd2mdu	1218	41	prepared	prepare	VBD
cord-014597-66vd2mdu	1218	42	as	as	IN
cord-014597-66vd2mdu	1218	43	for	for	IN
cord-014597-66vd2mdu	1218	44	encapsulation	encapsulation	NN
cord-014597-66vd2mdu	1218	45	.	.	.
cord-014597-66vd2mdu	1219	1	As	as	IN
cord-014597-66vd2mdu	1219	2	control	control	NN
cord-014597-66vd2mdu	1219	3	,	,	,
cord-014597-66vd2mdu	1219	4	the	the	DT
cord-014597-66vd2mdu	1219	5	SCS	SCS	NNP
cord-014597-66vd2mdu	1219	6	was	be	VBD
cord-014597-66vd2mdu	1219	7	replaced	replace	VBN
cord-014597-66vd2mdu	1219	8	by	by	IN
cord-014597-66vd2mdu	1219	9	PBS	PBS	NNP
cord-014597-66vd2mdu	1219	10	.	.	.
cord-014597-66vd2mdu	1220	1	Shown	show	VBN
cord-014597-66vd2mdu	1220	2	are	be	VBP
cord-014597-66vd2mdu	1220	3	mean	mean	JJ
cord-014597-66vd2mdu	1220	4	values	value	NNS
cord-014597-66vd2mdu	1220	5	±	±	NNP
cord-014597-66vd2mdu	1220	6	SD	SD	NNP
cord-014597-66vd2mdu	1220	7	,	,	,
cord-014597-66vd2mdu	1220	8	n	n	NN
cord-014597-66vd2mdu	1220	9	=	=	SYM
cord-014597-66vd2mdu	1220	10	3	3	CD
cord-014597-66vd2mdu	1220	11	analysis	analysis	NN
cord-014597-66vd2mdu	1220	12	including	include	VBG
cord-014597-66vd2mdu	1220	13	software	software	NN
cord-014597-66vd2mdu	1220	14	sensors	sensor	NNS
cord-014597-66vd2mdu	1220	15	for	for	IN
cord-014597-66vd2mdu	1220	16	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1220	17	fluxes	flux	NNS
cord-014597-66vd2mdu	1220	18	,	,	,
cord-014597-66vd2mdu	1220	19	and	and	CC
cord-014597-66vd2mdu	1220	20	model	model	NN
cord-014597-66vd2mdu	1220	21	-	-	HYPH
cord-014597-66vd2mdu	1220	22	based	base	VBN
cord-014597-66vd2mdu	1220	23	predictions	prediction	NNS
cord-014597-66vd2mdu	1220	24	of	of	IN
cord-014597-66vd2mdu	1220	25	digital	digital	JJ
cord-014597-66vd2mdu	1220	26	biomanufacturing	biomanufacturing	NN
cord-014597-66vd2mdu	1220	27	.	.	.
cord-014597-66vd2mdu	1221	1	The	the	DT
cord-014597-66vd2mdu	1221	2	application	application	NN
cord-014597-66vd2mdu	1221	3	of	of	IN
cord-014597-66vd2mdu	1221	4	predictive	predictive	JJ
cord-014597-66vd2mdu	1221	5	models	model	NNS
cord-014597-66vd2mdu	1221	6	for	for	IN
cord-014597-66vd2mdu	1221	7	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	1221	8	optimization	optimization	NN
cord-014597-66vd2mdu	1221	9	greatly	greatly	RB
cord-014597-66vd2mdu	1221	10	improves	improve	VBZ
cord-014597-66vd2mdu	1221	11	established	establish	VBN
cord-014597-66vd2mdu	1221	12	platforms	platform	NNS
cord-014597-66vd2mdu	1221	13	and	and	CC
cord-014597-66vd2mdu	1221	14	finally	finally	RB
cord-014597-66vd2mdu	1221	15	leads	lead	VBZ
cord-014597-66vd2mdu	1221	16	to	to	IN
cord-014597-66vd2mdu	1221	17	a	a	DT
cord-014597-66vd2mdu	1221	18	massively	massively	RB
cord-014597-66vd2mdu	1221	19	increased	increase	VBN
cord-014597-66vd2mdu	1221	20	mechanistic	mechanistic	JJ
cord-014597-66vd2mdu	1221	21	process	process	NN
cord-014597-66vd2mdu	1221	22	understanding	understanding	NN
cord-014597-66vd2mdu	1221	23	.	.	.
cord-014597-66vd2mdu	1222	1	Four	four	CD
cord-014597-66vd2mdu	1222	2	essential	essential	JJ
cord-014597-66vd2mdu	1222	3	benefits	benefit	NNS
cord-014597-66vd2mdu	1222	4	result	result	VBP
cord-014597-66vd2mdu	1222	5	from	from	IN
cord-014597-66vd2mdu	1222	6	the	the	DT
cord-014597-66vd2mdu	1222	7	increased	increase	VBN
cord-014597-66vd2mdu	1222	8	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	1222	9	understanding	understanding	NN
cord-014597-66vd2mdu	1222	10	,	,	,
cord-014597-66vd2mdu	1222	11	development	development	NN
cord-014597-66vd2mdu	1222	12	,	,	,
cord-014597-66vd2mdu	1222	13	and	and	CC
cord-014597-66vd2mdu	1222	14	control	control	NN
cord-014597-66vd2mdu	1222	15	of	of	IN
cord-014597-66vd2mdu	1222	16	DB	DB	NNP
cord-014597-66vd2mdu	1222	17	.	.	.
cord-014597-66vd2mdu	1223	1	First	first	RB
cord-014597-66vd2mdu	1223	2	,	,	,
cord-014597-66vd2mdu	1223	3	personnel	personnel	NNS
cord-014597-66vd2mdu	1223	4	are	be	VBP
cord-014597-66vd2mdu	1223	5	relieved	relieve	VBN
cord-014597-66vd2mdu	1223	6	of	of	IN
cord-014597-66vd2mdu	1223	7	many	many	JJ
cord-014597-66vd2mdu	1223	8	manual	manual	JJ
cord-014597-66vd2mdu	1223	9	and	and	CC
cord-014597-66vd2mdu	1223	10	repetitive	repetitive	JJ
cord-014597-66vd2mdu	1223	11	tasks	task	NNS
cord-014597-66vd2mdu	1223	12	.	.	.
cord-014597-66vd2mdu	1224	1	Second	second	JJ
cord-014597-66vd2mdu	1224	2	,	,	,
cord-014597-66vd2mdu	1224	3	strategic	strategic	JJ
cord-014597-66vd2mdu	1224	4	planning	planning	NN
cord-014597-66vd2mdu	1224	5	and	and	CC
cord-014597-66vd2mdu	1224	6	operational	operational	JJ
cord-014597-66vd2mdu	1224	7	efficiency	efficiency	NN
cord-014597-66vd2mdu	1224	8	are	be	VBP
cord-014597-66vd2mdu	1224	9	improved	improve	VBN
cord-014597-66vd2mdu	1224	10	.	.	.
cord-014597-66vd2mdu	1225	1	Third	third	RB
cord-014597-66vd2mdu	1225	2	,	,	,
cord-014597-66vd2mdu	1225	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1225	4	see	see	VBP
cord-014597-66vd2mdu	1225	5	real	real	JJ
cord-014597-66vd2mdu	1225	6	-	-	HYPH
cord-014597-66vd2mdu	1225	7	time	time	NN
cord-014597-66vd2mdu	1225	8	optimization	optimization	NN
cord-014597-66vd2mdu	1225	9	of	of	IN
cord-014597-66vd2mdu	1225	10	end	end	NN
cord-014597-66vd2mdu	1225	11	-	-	HYPH
cord-014597-66vd2mdu	1225	12	to	to	IN
cord-014597-66vd2mdu	1225	13	-	-	HYPH
cord-014597-66vd2mdu	1225	14	end	end	NN
cord-014597-66vd2mdu	1225	15	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1225	16	based	base	VBN
cord-014597-66vd2mdu	1225	17	on	on	IN
cord-014597-66vd2mdu	1225	18	such	such	JJ
cord-014597-66vd2mdu	1225	19	high	high	JJ
cord-014597-66vd2mdu	1225	20	-	-	HYPH
cord-014597-66vd2mdu	1225	21	value	value	NN
cord-014597-66vd2mdu	1225	22	criteria	criterion	NNS
cord-014597-66vd2mdu	1225	23	as	as	IN
cord-014597-66vd2mdu	1225	24	projected	project	VBN
cord-014597-66vd2mdu	1225	25	product	product	NN
cord-014597-66vd2mdu	1225	26	quality	quality	NN
cord-014597-66vd2mdu	1225	27	and	and	CC
cord-014597-66vd2mdu	1225	28	profitability	profitability	NN
cord-014597-66vd2mdu	1225	29	.	.	.
cord-014597-66vd2mdu	1226	1	Fourth	fourth	RB
cord-014597-66vd2mdu	1226	2	,	,	,
cord-014597-66vd2mdu	1226	3	it	-PRON-	PRP
cord-014597-66vd2mdu	1226	4	enables	enable	VBZ
cord-014597-66vd2mdu	1226	5	previously	previously	RB
cord-014597-66vd2mdu	1226	6	unmanageable	unmanageable	JJ
cord-014597-66vd2mdu	1226	7	operations	operation	NNS
cord-014597-66vd2mdu	1226	8	and	and	CC
cord-014597-66vd2mdu	1226	9	creates	create	VBZ
cord-014597-66vd2mdu	1226	10	innovative	innovative	JJ
cord-014597-66vd2mdu	1226	11	solutions	solution	NNS
cord-014597-66vd2mdu	1226	12	.	.	.
cord-014597-66vd2mdu	1227	1	Monitoring	monitor	VBG
cord-014597-66vd2mdu	1227	2	between	between	IN
cord-014597-66vd2mdu	1227	3	-	-	HYPH
cord-014597-66vd2mdu	1227	4	batch	batch	NN
cord-014597-66vd2mdu	1227	5	behavior	behavior	NN
cord-014597-66vd2mdu	1227	6	of	of	IN
cord-014597-66vd2mdu	1227	7	real	real	JJ
cord-014597-66vd2mdu	1227	8	-	-	HYPH
cord-014597-66vd2mdu	1227	9	time	time	NN
cord-014597-66vd2mdu	1227	10	adjusted	adjust	VBN
cord-014597-66vd2mdu	1227	11	cellculture	cellculture	NN
cord-014597-66vd2mdu	1227	12	parameters	parameter	NNS
cord-014597-66vd2mdu	1227	13	Xavier	Xavier	NNP
cord-014597-66vd2mdu	1227	14	Lories	Lories	NNP
cord-014597-66vd2mdu	1227	15	,	,	,
cord-014597-66vd2mdu	1227	16	Jean	Jean	NNP
cord-014597-66vd2mdu	1227	17	-	-	HYPH
cord-014597-66vd2mdu	1227	18	François	François	NNP
cord-014597-66vd2mdu	1227	19	Michiels	Michiels	NNP
cord-014597-66vd2mdu	1227	20	Arlenda	Arlenda	NNP
cord-014597-66vd2mdu	1227	21	,	,	,
cord-014597-66vd2mdu	1227	22	Mont	Mont	NNP
cord-014597-66vd2mdu	1227	23	-	-	HYPH
cord-014597-66vd2mdu	1227	24	Saint	Saint	NNP
cord-014597-66vd2mdu	1227	25	-	-	HYPH
cord-014597-66vd2mdu	1227	26	Guibert	Guibert	NNP
cord-014597-66vd2mdu	1227	27	,	,	,
cord-014597-66vd2mdu	1227	28	1435	1435	CD
cord-014597-66vd2mdu	1227	29	,	,	,
cord-014597-66vd2mdu	1227	30	Belgium	Belgium	NNP
cord-014597-66vd2mdu	1227	31	Correspondence	Correspondence	NNP
cord-014597-66vd2mdu	1227	32	:	:	:
cord-014597-66vd2mdu	1228	1	Xavier	Xavier	NNP
cord-014597-66vd2mdu	1228	2	Lories	Lories	NNP
cord-014597-66vd2mdu	1228	3	(	(	-LRB-
cord-014597-66vd2mdu	1228	4	xavier.lories@arlenda.com	xavier.lories@arlenda.com	ADD
cord-014597-66vd2mdu	1228	5	)	)	-RRB-
cord-014597-66vd2mdu	1229	1	BMC	BMC	NNP
cord-014597-66vd2mdu	1229	2	Proceedings	Proceedings	NNP
cord-014597-66vd2mdu	1229	3	2018	2018	CD
cord-014597-66vd2mdu	1229	4	,	,	,
cord-014597-66vd2mdu	1230	1	12(Suppl	12(suppl	CD
cord-014597-66vd2mdu	1230	2	1):P-192	1):p-192	CD
cord-014597-66vd2mdu	1231	1	Background	Background	NNP
cord-014597-66vd2mdu	1231	2	Cell	cell	NN
cord-014597-66vd2mdu	1231	3	-	-	HYPH
cord-014597-66vd2mdu	1231	4	culture	culture	NN
cord-014597-66vd2mdu	1231	5	parameters	parameter	NNS
cord-014597-66vd2mdu	1231	6	(	(	-LRB-
cord-014597-66vd2mdu	1231	7	CCP	CCP	NNP
cord-014597-66vd2mdu	1231	8	)	)	-RRB-
cord-014597-66vd2mdu	1231	9	,	,	,
cord-014597-66vd2mdu	1231	10	such	such	JJ
cord-014597-66vd2mdu	1231	11	as	as	IN
cord-014597-66vd2mdu	1231	12	pH	pH	NNP
cord-014597-66vd2mdu	1231	13	,	,	,
cord-014597-66vd2mdu	1231	14	may	may	MD
cord-014597-66vd2mdu	1231	15	be	be	VB
cord-014597-66vd2mdu	1231	16	continuously	continuously	RB
cord-014597-66vd2mdu	1231	17	measured	measure	VBN
cord-014597-66vd2mdu	1231	18	online	online	RB
cord-014597-66vd2mdu	1231	19	and	and	CC
cord-014597-66vd2mdu	1231	20	subject	subject	JJ
cord-014597-66vd2mdu	1231	21	to	to	IN
cord-014597-66vd2mdu	1231	22	real	real	JJ
cord-014597-66vd2mdu	1231	23	-	-	HYPH
cord-014597-66vd2mdu	1231	24	time	time	NN
cord-014597-66vd2mdu	1231	25	automated	automated	JJ
cord-014597-66vd2mdu	1231	26	adjustment	adjustment	NN
cord-014597-66vd2mdu	1231	27	(	(	-LRB-
cord-014597-66vd2mdu	1231	28	e.g.	e.g.	RB
cord-014597-66vd2mdu	1231	29	automated	automate	VBN
cord-014597-66vd2mdu	1231	30	addition	addition	NN
cord-014597-66vd2mdu	1231	31	of	of	IN
cord-014597-66vd2mdu	1231	32	a	a	DT
cord-014597-66vd2mdu	1231	33	base	base	NN
cord-014597-66vd2mdu	1231	34	to	to	TO
cord-014597-66vd2mdu	1231	35	prevent	prevent	VB
cord-014597-66vd2mdu	1231	36	the	the	DT
cord-014597-66vd2mdu	1231	37	pH	pH	NNP
cord-014597-66vd2mdu	1231	38	to	to	TO
cord-014597-66vd2mdu	1231	39	drop	drop	VB
cord-014597-66vd2mdu	1231	40	too	too	RB
cord-014597-66vd2mdu	1231	41	low	low	JJ
cord-014597-66vd2mdu	1231	42	)	)	-RRB-
cord-014597-66vd2mdu	1231	43	.	.	.
cord-014597-66vd2mdu	1232	1	This	this	DT
cord-014597-66vd2mdu	1232	2	is	be	VBZ
cord-014597-66vd2mdu	1232	3	an	an	DT
cord-014597-66vd2mdu	1232	4	efficient	efficient	JJ
cord-014597-66vd2mdu	1232	5	method	method	NN
cord-014597-66vd2mdu	1232	6	to	to	TO
cord-014597-66vd2mdu	1232	7	maintain	maintain	VB
cord-014597-66vd2mdu	1232	8	the	the	DT
cord-014597-66vd2mdu	1232	9	parameter	parameter	NN
cord-014597-66vd2mdu	1232	10	within	within	IN
cord-014597-66vd2mdu	1232	11	specified	specified	JJ
cord-014597-66vd2mdu	1232	12	limits	limit	NNS
cord-014597-66vd2mdu	1232	13	.	.	.
cord-014597-66vd2mdu	1233	1	This	this	DT
cord-014597-66vd2mdu	1233	2	type	type	NN
cord-014597-66vd2mdu	1233	3	of	of	IN
cord-014597-66vd2mdu	1233	4	control	control	NN
cord-014597-66vd2mdu	1233	5	constraint	constraint	NN
cord-014597-66vd2mdu	1233	6	the	the	DT
cord-014597-66vd2mdu	1233	7	variability	variability	NN
cord-014597-66vd2mdu	1233	8	within	within	IN
cord-014597-66vd2mdu	1233	9	the	the	DT
cord-014597-66vd2mdu	1233	10	predefined	predefined	JJ
cord-014597-66vd2mdu	1233	11	limits	limit	NNS
cord-014597-66vd2mdu	1233	12	and	and	CC
cord-014597-66vd2mdu	1233	13	does	do	VBZ
cord-014597-66vd2mdu	1233	14	not	not	RB
cord-014597-66vd2mdu	1233	15	provide	provide	VB
cord-014597-66vd2mdu	1233	16	any	any	DT
cord-014597-66vd2mdu	1233	17	information	information	NN
cord-014597-66vd2mdu	1233	18	on	on	IN
cord-014597-66vd2mdu	1233	19	the	the	DT
cord-014597-66vd2mdu	1233	20	between	between	IN
cord-014597-66vd2mdu	1233	21	-	-	HYPH
cord-014597-66vd2mdu	1233	22	batch	batch	NN
cord-014597-66vd2mdu	1233	23	variability	variability	NN
cord-014597-66vd2mdu	1233	24	of	of	IN
cord-014597-66vd2mdu	1233	25	the	the	DT
cord-014597-66vd2mdu	1233	26	process	process	NN
cord-014597-66vd2mdu	1233	27	.	.	.
cord-014597-66vd2mdu	1234	1	Online	online	JJ
cord-014597-66vd2mdu	1234	2	measurements	measurement	NNS
cord-014597-66vd2mdu	1234	3	of	of	IN
cord-014597-66vd2mdu	1234	4	CCP	CCP	NNP
cord-014597-66vd2mdu	1234	5	provide	provide	VB
cord-014597-66vd2mdu	1234	6	time	time	NN
cord-014597-66vd2mdu	1234	7	-	-	HYPH
cord-014597-66vd2mdu	1234	8	dependent	dependent	JJ
cord-014597-66vd2mdu	1234	9	curves	curve	NNS
cord-014597-66vd2mdu	1234	10	presenting	present	VBG
cord-014597-66vd2mdu	1234	11	one	one	CD
cord-014597-66vd2mdu	1234	12	or	or	CC
cord-014597-66vd2mdu	1234	13	more	more	JJR
cord-014597-66vd2mdu	1234	14	transitions	transition	NNS
cord-014597-66vd2mdu	1234	15	.	.	.
cord-014597-66vd2mdu	1235	1	Different	different	JJ
cord-014597-66vd2mdu	1235	2	types	type	NNS
cord-014597-66vd2mdu	1235	3	of	of	IN
cord-014597-66vd2mdu	1235	4	transition	transition	NN
cord-014597-66vd2mdu	1235	5	can	can	MD
cord-014597-66vd2mdu	1235	6	be	be	VB
cord-014597-66vd2mdu	1235	7	observed	observe	VBN
cord-014597-66vd2mdu	1235	8	:	:	:
cord-014597-66vd2mdu	1235	9	-The	-the	DT
cord-014597-66vd2mdu	1235	10	process	process	NN
cord-014597-66vd2mdu	1235	11	can	can	MD
cord-014597-66vd2mdu	1235	12	shift	shift	VB
cord-014597-66vd2mdu	1235	13	from	from	IN
cord-014597-66vd2mdu	1235	14	a	a	DT
cord-014597-66vd2mdu	1235	15	state	state	NN
cord-014597-66vd2mdu	1235	16	in	in	IN
cord-014597-66vd2mdu	1235	17	which	which	WDT
cord-014597-66vd2mdu	1235	18	adjustment	adjustment	NN
cord-014597-66vd2mdu	1235	19	is	be	VBZ
cord-014597-66vd2mdu	1235	20	needed	need	VBN
cord-014597-66vd2mdu	1235	21	to	to	TO
cord-014597-66vd2mdu	1235	22	keep	keep	VB
cord-014597-66vd2mdu	1235	23	the	the	DT
cord-014597-66vd2mdu	1235	24	CCP	CCP	NNP
cord-014597-66vd2mdu	1235	25	in	in	IN
cord-014597-66vd2mdu	1235	26	range	range	NN
cord-014597-66vd2mdu	1235	27	to	to	IN
cord-014597-66vd2mdu	1235	28	a	a	DT
cord-014597-66vd2mdu	1235	29	state	state	NN
cord-014597-66vd2mdu	1235	30	in	in	IN
cord-014597-66vd2mdu	1235	31	which	which	WDT
cord-014597-66vd2mdu	1235	32	it	-PRON-	PRP
cord-014597-66vd2mdu	1235	33	is	be	VBZ
cord-014597-66vd2mdu	1235	34	not	not	RB
cord-014597-66vd2mdu	1235	35	.	.	.
cord-014597-66vd2mdu	1236	1	Typically	typically	RB
cord-014597-66vd2mdu	1236	2	,	,	,
cord-014597-66vd2mdu	1236	3	the	the	DT
cord-014597-66vd2mdu	1236	4	CCP	CCP	NNP
cord-014597-66vd2mdu	1236	5	drifts	drift	VBZ
cord-014597-66vd2mdu	1236	6	away	away	RB
cord-014597-66vd2mdu	1236	7	from	from	IN
cord-014597-66vd2mdu	1236	8	a	a	DT
cord-014597-66vd2mdu	1236	9	limit	limit	NN
cord-014597-66vd2mdu	1236	10	.	.	.
cord-014597-66vd2mdu	1237	1	-The	-the	DT
cord-014597-66vd2mdu	1237	2	process	process	NN
cord-014597-66vd2mdu	1237	3	shifts	shift	VBZ
cord-014597-66vd2mdu	1237	4	from	from	IN
cord-014597-66vd2mdu	1237	5	a	a	DT
cord-014597-66vd2mdu	1237	6	state	state	NN
cord-014597-66vd2mdu	1237	7	in	in	IN
cord-014597-66vd2mdu	1237	8	which	which	WDT
cord-014597-66vd2mdu	1237	9	adjustment	adjustment	NN
cord-014597-66vd2mdu	1237	10	is	be	VBZ
cord-014597-66vd2mdu	1237	11	not	not	RB
cord-014597-66vd2mdu	1237	12	needed	need	VBN
cord-014597-66vd2mdu	1237	13	to	to	IN
cord-014597-66vd2mdu	1237	14	one	one	CD
cord-014597-66vd2mdu	1237	15	in	in	IN
cord-014597-66vd2mdu	1237	16	which	which	WDT
cord-014597-66vd2mdu	1237	17	it	-PRON-	PRP
cord-014597-66vd2mdu	1237	18	is	be	VBZ
cord-014597-66vd2mdu	1237	19	.	.	.
cord-014597-66vd2mdu	1238	1	For	for	IN
cord-014597-66vd2mdu	1238	2	instance	instance	NN
cord-014597-66vd2mdu	1238	3	,	,	,
cord-014597-66vd2mdu	1238	4	a	a	DT
cord-014597-66vd2mdu	1238	5	drifting	drift	VBG
cord-014597-66vd2mdu	1238	6	CCP	CCP	NNP
cord-014597-66vd2mdu	1238	7	reaches	reach	VBZ
cord-014597-66vd2mdu	1238	8	the	the	DT
cord-014597-66vd2mdu	1238	9	lower	low	JJR
cord-014597-66vd2mdu	1238	10	or	or	CC
cord-014597-66vd2mdu	1238	11	upper	upper	JJ
cord-014597-66vd2mdu	1238	12	limit	limit	NN
cord-014597-66vd2mdu	1238	13	of	of	IN
cord-014597-66vd2mdu	1238	14	the	the	DT
cord-014597-66vd2mdu	1238	15	accepted	accept	VBN
cord-014597-66vd2mdu	1238	16	range	range	NN
cord-014597-66vd2mdu	1238	17	.	.	.
cord-014597-66vd2mdu	1239	1	The	the	DT
cord-014597-66vd2mdu	1239	2	timepoints	timepoint	NNS
cord-014597-66vd2mdu	1239	3	at	at	IN
cord-014597-66vd2mdu	1239	4	which	which	WDT
cord-014597-66vd2mdu	1239	5	those	those	DT
cord-014597-66vd2mdu	1239	6	transitions	transition	NNS
cord-014597-66vd2mdu	1239	7	take	take	VBP
cord-014597-66vd2mdu	1239	8	place	place	NN
cord-014597-66vd2mdu	1239	9	are	be	VBP
cord-014597-66vd2mdu	1239	10	here	here	RB
cord-014597-66vd2mdu	1239	11	called	call	VBN
cord-014597-66vd2mdu	1239	12	changepoints	changepoint	NNS
cord-014597-66vd2mdu	1239	13	.	.	.
cord-014597-66vd2mdu	1240	1	Those	those	DT
cord-014597-66vd2mdu	1240	2	are	be	VBP
cord-014597-66vd2mdu	1240	3	aspects	aspect	NNS
cord-014597-66vd2mdu	1240	4	of	of	IN
cord-014597-66vd2mdu	1240	5	the	the	DT
cord-014597-66vd2mdu	1240	6	process	process	NN
cord-014597-66vd2mdu	1240	7	and	and	CC
cord-014597-66vd2mdu	1240	8	,	,	,
cord-014597-66vd2mdu	1240	9	as	as	IN
cord-014597-66vd2mdu	1240	10	such	such	JJ
cord-014597-66vd2mdu	1240	11	,	,	,
cord-014597-66vd2mdu	1240	12	should	should	MD
cord-014597-66vd2mdu	1240	13	be	be	VB
cord-014597-66vd2mdu	1240	14	controlled	control	VBN
cord-014597-66vd2mdu	1240	15	.	.	.
cord-014597-66vd2mdu	1241	1	In	in	IN
cord-014597-66vd2mdu	1241	2	the	the	DT
cord-014597-66vd2mdu	1241	3	multiple	multiple	JJ
cord-014597-66vd2mdu	1241	4	changepoints	changepoint	NNS
cord-014597-66vd2mdu	1241	5	cases	case	NNS
cord-014597-66vd2mdu	1241	6	,	,	,
cord-014597-66vd2mdu	1241	7	the	the	DT
cord-014597-66vd2mdu	1241	8	approach	approach	NN
cord-014597-66vd2mdu	1241	9	allows	allow	VBZ
cord-014597-66vd2mdu	1241	10	the	the	DT
cord-014597-66vd2mdu	1241	11	early	early	JJ
cord-014597-66vd2mdu	1241	12	termination	termination	NN
cord-014597-66vd2mdu	1241	13	of	of	IN
cord-014597-66vd2mdu	1241	14	runs	run	NNS
cord-014597-66vd2mdu	1241	15	showing	show	VBG
cord-014597-66vd2mdu	1241	16	very	very	RB
cord-014597-66vd2mdu	1241	17	early	early	RB
cord-014597-66vd2mdu	1241	18	or	or	CC
cord-014597-66vd2mdu	1241	19	very	very	RB
cord-014597-66vd2mdu	1241	20	late	late	JJ
cord-014597-66vd2mdu	1241	21	first	first	JJ
cord-014597-66vd2mdu	1241	22	changepoint	changepoint	NN
cord-014597-66vd2mdu	1241	23	.	.	.
cord-014597-66vd2mdu	1242	1	The	the	DT
cord-014597-66vd2mdu	1242	2	identification	identification	NN
cord-014597-66vd2mdu	1242	3	of	of	IN
cord-014597-66vd2mdu	1242	4	the	the	DT
cord-014597-66vd2mdu	1242	5	changepoints	changepoint	NNS
cord-014597-66vd2mdu	1242	6	position	position	NN
cord-014597-66vd2mdu	1242	7	is	be	VBZ
cord-014597-66vd2mdu	1242	8	based	base	VBN
cord-014597-66vd2mdu	1242	9	on	on	IN
cord-014597-66vd2mdu	1242	10	simple	simple	JJ
cord-014597-66vd2mdu	1242	11	rules	rule	NNS
cord-014597-66vd2mdu	1242	12	rather	rather	RB
cord-014597-66vd2mdu	1242	13	than	than	IN
cord-014597-66vd2mdu	1242	14	complex	complex	JJ
cord-014597-66vd2mdu	1242	15	statistical	statistical	JJ
cord-014597-66vd2mdu	1242	16	modeling	modeling	NN
cord-014597-66vd2mdu	1242	17	to	to	TO
cord-014597-66vd2mdu	1242	18	keep	keep	VB
cord-014597-66vd2mdu	1242	19	the	the	DT
cord-014597-66vd2mdu	1242	20	identification	identification	NN
cord-014597-66vd2mdu	1242	21	methodology	methodology	NN
cord-014597-66vd2mdu	1242	22	simple	simple	JJ
cord-014597-66vd2mdu	1242	23	.	.	.
cord-014597-66vd2mdu	1243	1	Once	once	IN
cord-014597-66vd2mdu	1243	2	the	the	DT
cord-014597-66vd2mdu	1243	3	changepoint	changepoint	NN
cord-014597-66vd2mdu	1243	4	are	be	VBP
cord-014597-66vd2mdu	1243	5	identified	identify	VBN
cord-014597-66vd2mdu	1243	6	,	,	,
cord-014597-66vd2mdu	1243	7	a	a	DT
cord-014597-66vd2mdu	1243	8	multivariate	multivariate	JJ
cord-014597-66vd2mdu	1243	9	Bayesian	bayesian	JJ
cord-014597-66vd2mdu	1243	10	model	model	NN
cord-014597-66vd2mdu	1243	11	is	be	VBZ
cord-014597-66vd2mdu	1243	12	adjusted	adjust	VBN
cord-014597-66vd2mdu	1243	13	on	on	IN
cord-014597-66vd2mdu	1243	14	the	the	DT
cord-014597-66vd2mdu	1243	15	appropriately	appropriately	RB
cord-014597-66vd2mdu	1243	16	transformed	transform	VBN
cord-014597-66vd2mdu	1243	17	data	datum	NNS
cord-014597-66vd2mdu	1243	18	.	.	.
cord-014597-66vd2mdu	1244	1	Prediction	prediction	NN
cord-014597-66vd2mdu	1244	2	regions	region	NNS
cord-014597-66vd2mdu	1244	3	are	be	VBP
cord-014597-66vd2mdu	1244	4	obtained	obtain	VBN
cord-014597-66vd2mdu	1244	5	and	and	CC
cord-014597-66vd2mdu	1244	6	used	use	VBN
cord-014597-66vd2mdu	1244	7	as	as	IN
cord-014597-66vd2mdu	1244	8	control	control	NN
cord-014597-66vd2mdu	1244	9	limits	limit	NNS
cord-014597-66vd2mdu	1244	10	[	[	-LRB-
cord-014597-66vd2mdu	1244	11	1	1	CD
cord-014597-66vd2mdu	1244	12	]	]	-RRB-
cord-014597-66vd2mdu	1244	13	.	.	.
cord-014597-66vd2mdu	1245	1	Results	result	NNS
cord-014597-66vd2mdu	1245	2	obtained	obtain	VBN
cord-014597-66vd2mdu	1245	3	for	for	IN
cord-014597-66vd2mdu	1245	4	a	a	DT
cord-014597-66vd2mdu	1245	5	2-changepoint	2-changepoint	CD
cord-014597-66vd2mdu	1245	6	case	case	NN
cord-014597-66vd2mdu	1245	7	are	be	VBP
cord-014597-66vd2mdu	1245	8	shown	show	VBN
cord-014597-66vd2mdu	1245	9	on	on	IN
cord-014597-66vd2mdu	1245	10	Fig	Fig	NNP
cord-014597-66vd2mdu	1245	11	.	.	.
cord-014597-66vd2mdu	1246	1	1	1	LS
cord-014597-66vd2mdu	1246	2	.	.	.
cord-014597-66vd2mdu	1247	1	Points	point	NNS
cord-014597-66vd2mdu	1247	2	on	on	IN
cord-014597-66vd2mdu	1247	3	the	the	DT
cord-014597-66vd2mdu	1247	4	right	right	JJ
cord-014597-66vd2mdu	1247	5	-	-	HYPH
cord-014597-66vd2mdu	1247	6	hand	hand	NN
cord-014597-66vd2mdu	1247	7	graph	graph	NN
cord-014597-66vd2mdu	1247	8	represent	represent	VBP
cord-014597-66vd2mdu	1247	9	new	new	JJ
cord-014597-66vd2mdu	1247	10	batches	batch	NNS
cord-014597-66vd2mdu	1247	11	.	.	.
cord-014597-66vd2mdu	1248	1	The	the	DT
cord-014597-66vd2mdu	1248	2	red	red	JJ
cord-014597-66vd2mdu	1248	3	triangle	triangle	NN
cord-014597-66vd2mdu	1248	4	represents	represent	VBZ
cord-014597-66vd2mdu	1248	5	a	a	DT
cord-014597-66vd2mdu	1248	6	failed	fail	VBN
cord-014597-66vd2mdu	1248	7	batch	batch	NN
cord-014597-66vd2mdu	1248	8	.	.	.
cord-014597-66vd2mdu	1249	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1249	2	appears	appear	VBZ
cord-014597-66vd2mdu	1249	3	that	that	IN
cord-014597-66vd2mdu	1249	4	the	the	DT
cord-014597-66vd2mdu	1249	5	control	control	NN
cord-014597-66vd2mdu	1249	6	strategy	strategy	NN
cord-014597-66vd2mdu	1249	7	fails	fail	VBZ
cord-014597-66vd2mdu	1249	8	to	to	TO
cord-014597-66vd2mdu	1249	9	identify	identify	VB
cord-014597-66vd2mdu	1249	10	the	the	DT
cord-014597-66vd2mdu	1249	11	failed	fail	VBN
cord-014597-66vd2mdu	1249	12	batch	batch	NN
cord-014597-66vd2mdu	1249	13	.	.	.
cord-014597-66vd2mdu	1250	1	Two	two	CD
cord-014597-66vd2mdu	1250	2	reasons	reason	NNS
cord-014597-66vd2mdu	1250	3	can	can	MD
cord-014597-66vd2mdu	1250	4	be	be	VB
cord-014597-66vd2mdu	1250	5	considered	consider	VBN
cord-014597-66vd2mdu	1250	6	:	:	:
cord-014597-66vd2mdu	1250	7	-The	-the	WDT
cord-014597-66vd2mdu	1250	8	limits	limit	NNS
cord-014597-66vd2mdu	1250	9	of	of	IN
cord-014597-66vd2mdu	1250	10	the	the	DT
cord-014597-66vd2mdu	1250	11	prediction	prediction	NN
cord-014597-66vd2mdu	1250	12	region	region	NN
cord-014597-66vd2mdu	1250	13	have	have	VBP
cord-014597-66vd2mdu	1250	14	been	be	VBN
cord-014597-66vd2mdu	1250	15	established	establish	VBN
cord-014597-66vd2mdu	1250	16	based	base	VBN
cord-014597-66vd2mdu	1250	17	on	on	IN
cord-014597-66vd2mdu	1250	18	9	9	CD
cord-014597-66vd2mdu	1250	19	points	point	NNS
cord-014597-66vd2mdu	1250	20	,	,	,
cord-014597-66vd2mdu	1250	21	such	such	PDT
cord-014597-66vd2mdu	1250	22	a	a	DT
cord-014597-66vd2mdu	1250	23	small	small	JJ
cord-014597-66vd2mdu	1250	24	sample	sample	NN
cord-014597-66vd2mdu	1250	25	size	size	NN
cord-014597-66vd2mdu	1250	26	is	be	VBZ
cord-014597-66vd2mdu	1250	27	likely	likely	JJ
cord-014597-66vd2mdu	1250	28	to	to	TO
cord-014597-66vd2mdu	1250	29	be	be	VB
cord-014597-66vd2mdu	1250	30	insufficient	insufficient	JJ
cord-014597-66vd2mdu	1250	31	for	for	IN
cord-014597-66vd2mdu	1250	32	the	the	DT
cord-014597-66vd2mdu	1250	33	definition	definition	NN
cord-014597-66vd2mdu	1250	34	of	of	IN
cord-014597-66vd2mdu	1250	35	such	such	PDT
cord-014597-66vd2mdu	1250	36	a	a	DT
cord-014597-66vd2mdu	1250	37	control	control	NN
cord-014597-66vd2mdu	1250	38	chart	chart	NN
cord-014597-66vd2mdu	1250	39	.	.	.
cord-014597-66vd2mdu	1251	1	-The	-The	:
cord-014597-66vd2mdu	1251	2	tested	test	VBN
cord-014597-66vd2mdu	1251	3	batches	batch	NNS
cord-014597-66vd2mdu	1251	4	were	be	VBD
cord-014597-66vd2mdu	1251	5	produced	produce	VBN
cord-014597-66vd2mdu	1251	6	out	out	IN
cord-014597-66vd2mdu	1251	7	of	of	IN
cord-014597-66vd2mdu	1251	8	set	set	NN
cord-014597-66vd2mdu	1251	9	point	point	NN
cord-014597-66vd2mdu	1251	10	.	.	.
cord-014597-66vd2mdu	1252	1	A	a	DT
cord-014597-66vd2mdu	1252	2	control	control	NN
cord-014597-66vd2mdu	1252	3	chart	chart	NN
cord-014597-66vd2mdu	1252	4	should	should	MD
cord-014597-66vd2mdu	1252	5	be	be	VB
cord-014597-66vd2mdu	1252	6	used	use	VBN
cord-014597-66vd2mdu	1252	7	on	on	IN
cord-014597-66vd2mdu	1252	8	a	a	DT
cord-014597-66vd2mdu	1252	9	stable	stable	JJ
cord-014597-66vd2mdu	1252	10	process	process	NN
cord-014597-66vd2mdu	1252	11	,	,	,
cord-014597-66vd2mdu	1252	12	ran	run	VBD
cord-014597-66vd2mdu	1252	13	in	in	IN
cord-014597-66vd2mdu	1252	14	the	the	DT
cord-014597-66vd2mdu	1252	15	same	same	JJ
cord-014597-66vd2mdu	1252	16	conditions	condition	NNS
cord-014597-66vd2mdu	1252	17	,	,	,
cord-014597-66vd2mdu	1252	18	in	in	IN
cord-014597-66vd2mdu	1252	19	order	order	NN
cord-014597-66vd2mdu	1252	20	to	to	TO
cord-014597-66vd2mdu	1252	21	be	be	VB
cord-014597-66vd2mdu	1252	22	really	really	RB
cord-014597-66vd2mdu	1252	23	relevant	relevant	JJ
cord-014597-66vd2mdu	1252	24	.	.	.
cord-014597-66vd2mdu	1253	1	This	this	DT
cord-014597-66vd2mdu	1253	2	work	work	NN
cord-014597-66vd2mdu	1253	3	was	be	VBD
cord-014597-66vd2mdu	1253	4	based	base	VBN
cord-014597-66vd2mdu	1253	5	on	on	IN
cord-014597-66vd2mdu	1253	6	available	available	JJ
cord-014597-66vd2mdu	1253	7	historical	historical	JJ
cord-014597-66vd2mdu	1253	8	data	datum	NNS
cord-014597-66vd2mdu	1253	9	,	,	,
cord-014597-66vd2mdu	1253	10	which	which	WDT
cord-014597-66vd2mdu	1253	11	is	be	VBZ
cord-014597-66vd2mdu	1253	12	never	never	RB
cord-014597-66vd2mdu	1253	13	an	an	DT
cord-014597-66vd2mdu	1253	14	ideal	ideal	JJ
cord-014597-66vd2mdu	1253	15	situation	situation	NN
cord-014597-66vd2mdu	1253	16	.	.	.
cord-014597-66vd2mdu	1254	1	The	the	DT
cord-014597-66vd2mdu	1254	2	suggested	suggested	JJ
cord-014597-66vd2mdu	1254	3	strategy	strategy	NN
cord-014597-66vd2mdu	1254	4	offers	offer	VBZ
cord-014597-66vd2mdu	1254	5	a	a	DT
cord-014597-66vd2mdu	1254	6	simple	simple	JJ
cord-014597-66vd2mdu	1254	7	approach	approach	NN
cord-014597-66vd2mdu	1254	8	to	to	IN
cord-014597-66vd2mdu	1254	9	the	the	DT
cord-014597-66vd2mdu	1254	10	monitoring	monitoring	NN
cord-014597-66vd2mdu	1254	11	of	of	IN
cord-014597-66vd2mdu	1254	12	between	between	IN
cord-014597-66vd2mdu	1254	13	-	-	HYPH
cord-014597-66vd2mdu	1254	14	batch	batch	NN
cord-014597-66vd2mdu	1254	15	behavior	behavior	NN
cord-014597-66vd2mdu	1254	16	for	for	IN
cord-014597-66vd2mdu	1254	17	cell	cell	NN
cord-014597-66vd2mdu	1254	18	-	-	HYPH
cord-014597-66vd2mdu	1254	19	culture	culture	NN
cord-014597-66vd2mdu	1254	20	.	.	.
cord-014597-66vd2mdu	1255	1	Once	once	IN
cord-014597-66vd2mdu	1255	2	the	the	DT
cord-014597-66vd2mdu	1255	3	limits	limit	NNS
cord-014597-66vd2mdu	1255	4	have	have	VBP
cord-014597-66vd2mdu	1255	5	been	be	VBN
cord-014597-66vd2mdu	1255	6	defined	define	VBN
cord-014597-66vd2mdu	1255	7	,	,	,
cord-014597-66vd2mdu	1255	8	the	the	DT
cord-014597-66vd2mdu	1255	9	approach	approach	NN
cord-014597-66vd2mdu	1255	10	is	be	VBZ
cord-014597-66vd2mdu	1255	11	quite	quite	RB
cord-014597-66vd2mdu	1255	12	straightforward	straightforward	JJ
cord-014597-66vd2mdu	1255	13	and	and	CC
cord-014597-66vd2mdu	1255	14	usable	usable	JJ
cord-014597-66vd2mdu	1255	15	by	by	IN
cord-014597-66vd2mdu	1255	16	nonstatistician	nonstatistician	NNP
cord-014597-66vd2mdu	1255	17	.	.	.
cord-014597-66vd2mdu	1256	1	However	however	RB
cord-014597-66vd2mdu	1256	2	,	,	,
cord-014597-66vd2mdu	1256	3	such	such	JJ
cord-014597-66vd2mdu	1256	4	strategy	strategy	NN
cord-014597-66vd2mdu	1256	5	,	,	,
cord-014597-66vd2mdu	1256	6	as	as	IN
cord-014597-66vd2mdu	1256	7	any	any	DT
cord-014597-66vd2mdu	1256	8	other	other	JJ
cord-014597-66vd2mdu	1256	9	of	of	IN
cord-014597-66vd2mdu	1256	10	this	this	DT
cord-014597-66vd2mdu	1256	11	type	type	NN
cord-014597-66vd2mdu	1256	12	,	,	,
cord-014597-66vd2mdu	1256	13	must	must	MD
cord-014597-66vd2mdu	1256	14	be	be	VB
cord-014597-66vd2mdu	1256	15	based	base	VBN
cord-014597-66vd2mdu	1256	16	on	on	IN
cord-014597-66vd2mdu	1256	17	a	a	DT
cord-014597-66vd2mdu	1256	18	sufficient	sufficient	JJ
cord-014597-66vd2mdu	1256	19	number	number	NN
cord-014597-66vd2mdu	1256	20	of	of	IN
cord-014597-66vd2mdu	1256	21	batches	batch	NNS
cord-014597-66vd2mdu	1256	22	for	for	IN
cord-014597-66vd2mdu	1256	23	the	the	DT
cord-014597-66vd2mdu	1256	24	definition	definition	NN
cord-014597-66vd2mdu	1256	25	of	of	IN
cord-014597-66vd2mdu	1256	26	the	the	DT
cord-014597-66vd2mdu	1256	27	control	control	NN
cord-014597-66vd2mdu	1256	28	limits	limit	NNS
cord-014597-66vd2mdu	1256	29	in	in	IN
cord-014597-66vd2mdu	1256	30	order	order	NN
cord-014597-66vd2mdu	1256	31	to	to	TO
cord-014597-66vd2mdu	1256	32	have	have	VB
cord-014597-66vd2mdu	1256	33	a	a	DT
cord-014597-66vd2mdu	1256	34	good	good	JJ
cord-014597-66vd2mdu	1256	35	estimation	estimation	NN
cord-014597-66vd2mdu	1256	36	of	of	IN
cord-014597-66vd2mdu	1256	37	the	the	DT
cord-014597-66vd2mdu	1256	38	batch	batch	NN
cord-014597-66vd2mdu	1256	39	-	-	HYPH
cord-014597-66vd2mdu	1256	40	to	to	TO
cord-014597-66vd2mdu	1256	41	batch	batch	VB
cord-014597-66vd2mdu	1256	42	variability	variability	NN
cord-014597-66vd2mdu	1256	43	.	.	.
cord-014597-66vd2mdu	1257	1	Fig	Fig	NNP
cord-014597-66vd2mdu	1257	2	.	.	.
cord-014597-66vd2mdu	1257	3	1	1	CD
cord-014597-66vd2mdu	1257	4	(	(	-LRB-
cord-014597-66vd2mdu	1257	5	abstract	abstract	JJ
cord-014597-66vd2mdu	1257	6	P-190	p-190	NN
cord-014597-66vd2mdu	1257	7	)	)	-RRB-
cord-014597-66vd2mdu	1257	8	.	.	.
cord-014597-66vd2mdu	1258	1	Intelligent	intelligent	JJ
cord-014597-66vd2mdu	1258	2	software	software	NN
cord-014597-66vd2mdu	1258	3	applications	application	NNS
cord-014597-66vd2mdu	1258	4	support	support	VBP
cord-014597-66vd2mdu	1258	5	digital	digital	JJ
cord-014597-66vd2mdu	1258	6	biomanufacturing	biomanufacturing	NN
cord-014597-66vd2mdu	1258	7	process	process	NN
cord-014597-66vd2mdu	1258	8	development	development	NN
cord-014597-66vd2mdu	1258	9	and	and	CC
cord-014597-66vd2mdu	1258	10	control	control	NN
cord-014597-66vd2mdu	1258	11	.	.	.
cord-014597-66vd2mdu	1259	1	•	•	NNP
cord-014597-66vd2mdu	1260	1	Databases	database	NNS
cord-014597-66vd2mdu	1260	2	using	use	VBG
cord-014597-66vd2mdu	1260	3	data	datum	NNS
cord-014597-66vd2mdu	1260	4	collected	collect	VBN
cord-014597-66vd2mdu	1260	5	online	online	RB
cord-014597-66vd2mdu	1260	6	,	,	,
cord-014597-66vd2mdu	1260	7	at	at	IN
cord-014597-66vd2mdu	1260	8	-	-	HYPH
cord-014597-66vd2mdu	1260	9	line	line	NN
cord-014597-66vd2mdu	1260	10	,	,	,
cord-014597-66vd2mdu	1260	11	and	and	CC
cord-014597-66vd2mdu	1260	12	offline	offline	JJ
cord-014597-66vd2mdu	1260	13	from	from	IN
cord-014597-66vd2mdu	1260	14	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	1260	15	operating	operate	VBG
cord-014597-66vd2mdu	1260	16	worldwide	worldwide	RB
cord-014597-66vd2mdu	1260	17	.	.	.
cord-014597-66vd2mdu	1261	1	•	•	NNP
cord-014597-66vd2mdu	1261	2	Process	Process	NNP
cord-014597-66vd2mdu	1261	3	data	datum	NNS
cord-014597-66vd2mdu	1261	4	are	be	VBP
cord-014597-66vd2mdu	1261	5	used	use	VBN
cord-014597-66vd2mdu	1261	6	to	to	TO
cord-014597-66vd2mdu	1261	7	generate	generate	VB
cord-014597-66vd2mdu	1261	8	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1261	9	network	network	NN
cord-014597-66vd2mdu	1261	10	models	model	NNS
cord-014597-66vd2mdu	1261	11	that	that	WDT
cord-014597-66vd2mdu	1261	12	represent	represent	VBP
cord-014597-66vd2mdu	1261	13	a	a	DT
cord-014597-66vd2mdu	1261	14	specific	specific	JJ
cord-014597-66vd2mdu	1261	15	host	host	NN
cord-014597-66vd2mdu	1261	16	cell	cell	NN
cord-014597-66vd2mdu	1261	17	line	line	NN
cord-014597-66vd2mdu	1261	18	in	in	IN
cord-014597-66vd2mdu	1261	19	a	a	DT
cord-014597-66vd2mdu	1261	20	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	1261	21	.	.	.
cord-014597-66vd2mdu	1262	1	•	•	NNP
cord-014597-66vd2mdu	1263	1	Modelbased	modelbased	JJ
cord-014597-66vd2mdu	1263	2	computational	computational	JJ
cord-014597-66vd2mdu	1263	3	simulations	simulation	NNS
cord-014597-66vd2mdu	1263	4	improve	improve	VBP
cord-014597-66vd2mdu	1263	5	process	process	NN
cord-014597-66vd2mdu	1263	6	understanding	understanding	NN
cord-014597-66vd2mdu	1263	7	and	and	CC
cord-014597-66vd2mdu	1263	8	reduce	reduce	VB
cord-014597-66vd2mdu	1263	9	experimental	experimental	JJ
cord-014597-66vd2mdu	1263	10	efforts	effort	NNS
cord-014597-66vd2mdu	1263	11	for	for	IN
cord-014597-66vd2mdu	1263	12	media	medium	NNS
cord-014597-66vd2mdu	1263	13	design	design	NN
cord-014597-66vd2mdu	1263	14	,	,	,
cord-014597-66vd2mdu	1263	15	clone	clone	NN
cord-014597-66vd2mdu	1263	16	selection	selection	NN
cord-014597-66vd2mdu	1263	17	,	,	,
cord-014597-66vd2mdu	1263	18	and	and	CC
cord-014597-66vd2mdu	1263	19	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1263	20	engineering	engineering	NN
cord-014597-66vd2mdu	1263	21	.	.	.
cord-014597-66vd2mdu	1264	1	•	•	NNP
cord-014597-66vd2mdu	1265	1	Automated	automate	VBN
cord-014597-66vd2mdu	1265	2	data	data	NN
cord-014597-66vd2mdu	1265	3	import	import	NN
cord-014597-66vd2mdu	1265	4	and	and	CC
cord-014597-66vd2mdu	1265	5	processing	processing	NN
cord-014597-66vd2mdu	1265	6	allow	allow	VB
cord-014597-66vd2mdu	1265	7	for	for	IN
cord-014597-66vd2mdu	1265	8	a	a	DT
cord-014597-66vd2mdu	1265	9	streamlined	streamlined	JJ
cord-014597-66vd2mdu	1265	10	and	and	CC
cord-014597-66vd2mdu	1265	11	standardized	standardized	JJ
cord-014597-66vd2mdu	1265	12	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1265	13	process	process	NN
cord-014597-66vd2mdu	1265	14	analysis	analysis	NN
cord-014597-66vd2mdu	1265	15	.	.	.
cord-014597-66vd2mdu	1266	1	•	•	NNP
cord-014597-66vd2mdu	1267	1	Identification	identification	NN
cord-014597-66vd2mdu	1267	2	of	of	IN
cord-014597-66vd2mdu	1267	3	critical	critical	JJ
cord-014597-66vd2mdu	1267	4	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1267	5	parameters	parameter	NNS
cord-014597-66vd2mdu	1267	6	is	be	VBZ
cord-014597-66vd2mdu	1267	7	used	use	VBN
cord-014597-66vd2mdu	1267	8	for	for	IN
cord-014597-66vd2mdu	1267	9	proactive	proactive	JJ
cord-014597-66vd2mdu	1267	10	steering	steering	NN
cord-014597-66vd2mdu	1267	11	and	and	CC
cord-014597-66vd2mdu	1267	12	control	control	NN
cord-014597-66vd2mdu	1267	13	of	of	IN
cord-014597-66vd2mdu	1267	14	production	production	NN
cord-014597-66vd2mdu	1267	15	processes	process	NNS
cord-014597-66vd2mdu	1268	1	Background	Background	NNP
cord-014597-66vd2mdu	1268	2	Rabies	Rabies	NNP
cord-014597-66vd2mdu	1268	3	is	be	VBZ
cord-014597-66vd2mdu	1268	4	a	a	DT
cord-014597-66vd2mdu	1268	5	zoonotic	zoonotic	JJ
cord-014597-66vd2mdu	1268	6	viral	viral	JJ
cord-014597-66vd2mdu	1268	7	disease	disease	NN
cord-014597-66vd2mdu	1268	8	with	with	IN
cord-014597-66vd2mdu	1268	9	a	a	DT
cord-014597-66vd2mdu	1268	10	mortality	mortality	NN
cord-014597-66vd2mdu	1268	11	close	close	JJ
cord-014597-66vd2mdu	1268	12	to	to	IN
cord-014597-66vd2mdu	1268	13	100	100	CD
cord-014597-66vd2mdu	1268	14	%	%	NN
cord-014597-66vd2mdu	1268	15	[	[	-LRB-
cord-014597-66vd2mdu	1268	16	1	1	CD
cord-014597-66vd2mdu	1268	17	]	]	-RRB-
cord-014597-66vd2mdu	1268	18	.	.	.
cord-014597-66vd2mdu	1269	1	As	as	IN
cord-014597-66vd2mdu	1269	2	there	there	EX
cord-014597-66vd2mdu	1269	3	is	be	VBZ
cord-014597-66vd2mdu	1269	4	not	not	RB
cord-014597-66vd2mdu	1269	5	an	an	DT
cord-014597-66vd2mdu	1269	6	efficacious	efficacious	JJ
cord-014597-66vd2mdu	1269	7	treatment	treatment	NN
cord-014597-66vd2mdu	1269	8	available	available	JJ
cord-014597-66vd2mdu	1269	9	,	,	,
cord-014597-66vd2mdu	1269	10	post	post	JJ
cord-014597-66vd2mdu	1269	11	-	-	JJ
cord-014597-66vd2mdu	1269	12	exposure	exposure	JJ
cord-014597-66vd2mdu	1269	13	vaccination	vaccination	NN
cord-014597-66vd2mdu	1269	14	is	be	VBZ
cord-014597-66vd2mdu	1269	15	recommended	recommend	VBN
cord-014597-66vd2mdu	1269	16	for	for	IN
cord-014597-66vd2mdu	1269	17	individuals	individual	NNS
cord-014597-66vd2mdu	1269	18	in	in	IN
cord-014597-66vd2mdu	1269	19	contact	contact	NN
cord-014597-66vd2mdu	1269	20	with	with	IN
cord-014597-66vd2mdu	1269	21	the	the	DT
cord-014597-66vd2mdu	1269	22	virus	virus	NN
cord-014597-66vd2mdu	1269	23	.	.	.
cord-014597-66vd2mdu	1270	1	On	on	IN
cord-014597-66vd2mdu	1270	2	the	the	DT
cord-014597-66vd2mdu	1270	3	other	other	JJ
cord-014597-66vd2mdu	1270	4	hand	hand	NN
cord-014597-66vd2mdu	1270	5	,	,	,
cord-014597-66vd2mdu	1270	6	the	the	DT
cord-014597-66vd2mdu	1270	7	most	most	RBS
cord-014597-66vd2mdu	1270	8	common	common	JJ
cord-014597-66vd2mdu	1270	9	source	source	NN
cord-014597-66vd2mdu	1270	10	of	of	IN
cord-014597-66vd2mdu	1270	11	virus	virus	NN
cord-014597-66vd2mdu	1270	12	transmission	transmission	NN
cord-014597-66vd2mdu	1270	13	is	be	VBZ
cord-014597-66vd2mdu	1270	14	saliva	saliva	NN
cord-014597-66vd2mdu	1270	15	of	of	IN
cord-014597-66vd2mdu	1270	16	infected	infected	JJ
cord-014597-66vd2mdu	1270	17	animals	animal	NNS
cord-014597-66vd2mdu	1270	18	,	,	,
cord-014597-66vd2mdu	1270	19	mostly	mostly	RB
cord-014597-66vd2mdu	1270	20	dogs	dog	NNS
cord-014597-66vd2mdu	1270	21	,	,	,
cord-014597-66vd2mdu	1270	22	whereby	whereby	WRB
cord-014597-66vd2mdu	1270	23	mass	mass	JJ
cord-014597-66vd2mdu	1270	24	vaccination	vaccination	NN
cord-014597-66vd2mdu	1270	25	of	of	IN
cord-014597-66vd2mdu	1270	26	pets	pet	NNS
cord-014597-66vd2mdu	1270	27	is	be	VBZ
cord-014597-66vd2mdu	1270	28	the	the	DT
cord-014597-66vd2mdu	1270	29	most	most	RBS
cord-014597-66vd2mdu	1270	30	cost	cost	NN
cord-014597-66vd2mdu	1270	31	-	-	HYPH
cord-014597-66vd2mdu	1270	32	effective	effective	JJ
cord-014597-66vd2mdu	1270	33	way	way	NN
cord-014597-66vd2mdu	1270	34	to	to	TO
cord-014597-66vd2mdu	1270	35	reduce	reduce	VB
cord-014597-66vd2mdu	1270	36	human	human	JJ
cord-014597-66vd2mdu	1270	37	infections	infection	NNS
cord-014597-66vd2mdu	1270	38	.	.	.
cord-014597-66vd2mdu	1271	1	In	in	IN
cord-014597-66vd2mdu	1271	2	this	this	DT
cord-014597-66vd2mdu	1271	3	context	context	NN
cord-014597-66vd2mdu	1271	4	,	,	,
cord-014597-66vd2mdu	1271	5	availability	availability	NN
cord-014597-66vd2mdu	1271	6	of	of	IN
cord-014597-66vd2mdu	1271	7	both	both	DT
cord-014597-66vd2mdu	1271	8	human	human	JJ
cord-014597-66vd2mdu	1271	9	and	and	CC
cord-014597-66vd2mdu	1271	10	veterinary	veterinary	JJ
cord-014597-66vd2mdu	1271	11	vaccines	vaccine	NNS
cord-014597-66vd2mdu	1271	12	is	be	VBZ
cord-014597-66vd2mdu	1271	13	critical	critical	JJ
cord-014597-66vd2mdu	1271	14	[	[	-LRB-
cord-014597-66vd2mdu	1271	15	2	2	CD
cord-014597-66vd2mdu	1271	16	,	,	,
cord-014597-66vd2mdu	1271	17	3	3	CD
cord-014597-66vd2mdu	1271	18	]	]	-RRB-
cord-014597-66vd2mdu	1271	19	.	.	.
cord-014597-66vd2mdu	1272	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	1272	2	group	group	NN
cord-014597-66vd2mdu	1272	3	had	have	VBD
cord-014597-66vd2mdu	1272	4	previously	previously	RB
cord-014597-66vd2mdu	1272	5	developed	develop	VBN
cord-014597-66vd2mdu	1272	6	an	an	DT
cord-014597-66vd2mdu	1272	7	effective	effective	JJ
cord-014597-66vd2mdu	1272	8	VLP	VLP	NNP
cord-014597-66vd2mdu	1272	9	-	-	HYPH
cord-014597-66vd2mdu	1272	10	based	base	VBN
cord-014597-66vd2mdu	1272	11	rabies	rabie	NNS
cord-014597-66vd2mdu	1272	12	vaccine	vaccine	NN
cord-014597-66vd2mdu	1272	13	candidate	candidate	NN
cord-014597-66vd2mdu	1272	14	produced	produce	VBN
cord-014597-66vd2mdu	1272	15	in	in	IN
cord-014597-66vd2mdu	1272	16	high	high	JJ
cord-014597-66vd2mdu	1272	17	density	density	NN
cord-014597-66vd2mdu	1272	18	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1272	19	cell	cell	NN
cord-014597-66vd2mdu	1272	20	cultures	culture	NNS
cord-014597-66vd2mdu	1272	21	with	with	IN
cord-014597-66vd2mdu	1272	22	serum	serum	NN
cord-014597-66vd2mdu	1272	23	free	free	JJ
cord-014597-66vd2mdu	1272	24	medium	medium	NN
cord-014597-66vd2mdu	1272	25	(	(	-LRB-
cord-014597-66vd2mdu	1272	26	SFM	SFM	NNP
cord-014597-66vd2mdu	1272	27	)	)	-RRB-
cord-014597-66vd2mdu	1272	28	[	[	-LRB-
cord-014597-66vd2mdu	1272	29	4	4	CD
cord-014597-66vd2mdu	1272	30	,	,	,
cord-014597-66vd2mdu	1272	31	5	5	CD
cord-014597-66vd2mdu	1272	32	]	]	-RRB-
cord-014597-66vd2mdu	1272	33	.	.	.
cord-014597-66vd2mdu	1273	1	One	one	CD
cord-014597-66vd2mdu	1273	2	of	of	IN
cord-014597-66vd2mdu	1273	3	the	the	DT
cord-014597-66vd2mdu	1273	4	aims	aim	NNS
cord-014597-66vd2mdu	1273	5	in	in	IN
cord-014597-66vd2mdu	1273	6	vaccine	vaccine	NN
cord-014597-66vd2mdu	1273	7	production	production	NN
cord-014597-66vd2mdu	1273	8	process	process	NN
cord-014597-66vd2mdu	1273	9	is	be	VBZ
cord-014597-66vd2mdu	1273	10	the	the	DT
cord-014597-66vd2mdu	1273	11	achievement	achievement	NN
cord-014597-66vd2mdu	1273	12	of	of	IN
cord-014597-66vd2mdu	1273	13	a	a	DT
cord-014597-66vd2mdu	1273	14	good	good	JJ
cord-014597-66vd2mdu	1273	15	productivity	productivity	NN
cord-014597-66vd2mdu	1273	16	with	with	IN
cord-014597-66vd2mdu	1273	17	a	a	DT
cord-014597-66vd2mdu	1273	18	low	low	JJ
cord-014597-66vd2mdu	1273	19	cost	cost	NN
cord-014597-66vd2mdu	1273	20	per	per	IN
cord-014597-66vd2mdu	1273	21	dose	dose	NN
cord-014597-66vd2mdu	1273	22	,	,	,
cord-014597-66vd2mdu	1273	23	mainly	mainly	RB
cord-014597-66vd2mdu	1273	24	in	in	IN
cord-014597-66vd2mdu	1273	25	the	the	DT
cord-014597-66vd2mdu	1273	26	case	case	NN
cord-014597-66vd2mdu	1273	27	of	of	IN
cord-014597-66vd2mdu	1273	28	vaccines	vaccine	NNS
cord-014597-66vd2mdu	1273	29	for	for	IN
cord-014597-66vd2mdu	1273	30	animal	animal	NN
cord-014597-66vd2mdu	1273	31	use	use	NN
cord-014597-66vd2mdu	1273	32	in	in	IN
cord-014597-66vd2mdu	1273	33	which	which	WDT
cord-014597-66vd2mdu	1273	34	case	case	NN
cord-014597-66vd2mdu	1273	35	the	the	DT
cord-014597-66vd2mdu	1273	36	SFM	SFM	NNP
cord-014597-66vd2mdu	1273	37	is	be	VBZ
cord-014597-66vd2mdu	1273	38	one	one	CD
cord-014597-66vd2mdu	1273	39	of	of	IN
cord-014597-66vd2mdu	1273	40	the	the	DT
cord-014597-66vd2mdu	1273	41	principal	principal	JJ
cord-014597-66vd2mdu	1273	42	expenses	expense	NNS
cord-014597-66vd2mdu	1273	43	.	.	.
cord-014597-66vd2mdu	1274	1	In	in	IN
cord-014597-66vd2mdu	1274	2	this	this	DT
cord-014597-66vd2mdu	1274	3	work	work	NN
cord-014597-66vd2mdu	1274	4	,	,	,
cord-014597-66vd2mdu	1274	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1274	6	show	show	VBP
cord-014597-66vd2mdu	1274	7	the	the	DT
cord-014597-66vd2mdu	1274	8	adaptation	adaptation	NN
cord-014597-66vd2mdu	1274	9	of	of	IN
cord-014597-66vd2mdu	1274	10	the	the	DT
cord-014597-66vd2mdu	1274	11	producer	producer	NN
cord-014597-66vd2mdu	1274	12	clone	clone	NN
cord-014597-66vd2mdu	1274	13	to	to	IN
cord-014597-66vd2mdu	1274	14	a	a	DT
cord-014597-66vd2mdu	1274	15	non	non	JJ
cord-014597-66vd2mdu	1274	16	-	-	JJ
cord-014597-66vd2mdu	1274	17	expensive	expensive	JJ
cord-014597-66vd2mdu	1274	18	in	in	IN
cord-014597-66vd2mdu	1274	19	-	-	HYPH
cord-014597-66vd2mdu	1274	20	house	house	NN
cord-014597-66vd2mdu	1274	21	developed	develop	VBN
cord-014597-66vd2mdu	1274	22	culture	culture	NN
cord-014597-66vd2mdu	1274	23	medium	medium	NN
cord-014597-66vd2mdu	1274	24	,	,	,
cord-014597-66vd2mdu	1274	25	in	in	IN
cord-014597-66vd2mdu	1274	26	order	order	NN
cord-014597-66vd2mdu	1274	27	to	to	TO
cord-014597-66vd2mdu	1274	28	reduce	reduce	VB
cord-014597-66vd2mdu	1274	29	the	the	DT
cord-014597-66vd2mdu	1274	30	global	global	JJ
cord-014597-66vd2mdu	1274	31	cost	cost	NN
cord-014597-66vd2mdu	1274	32	of	of	IN
cord-014597-66vd2mdu	1274	33	the	the	DT
cord-014597-66vd2mdu	1274	34	process	process	NN
cord-014597-66vd2mdu	1274	35	and	and	CC
cord-014597-66vd2mdu	1274	36	therefore	therefore	RB
cord-014597-66vd2mdu	1274	37	the	the	DT
cord-014597-66vd2mdu	1274	38	price	price	NN
cord-014597-66vd2mdu	1274	39	per	per	IN
cord-014597-66vd2mdu	1274	40	dose	dose	NN
cord-014597-66vd2mdu	1274	41	.	.	.
cord-014597-66vd2mdu	1275	1	Experimental	experimental	JJ
cord-014597-66vd2mdu	1275	2	approach	approach	NN
cord-014597-66vd2mdu	1276	1	First	first	RB
cord-014597-66vd2mdu	1276	2	,	,	,
cord-014597-66vd2mdu	1276	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1276	4	compared	compare	VBD
cord-014597-66vd2mdu	1276	5	a	a	DT
cord-014597-66vd2mdu	1276	6	direct	direct	JJ
cord-014597-66vd2mdu	1276	7	and	and	CC
cord-014597-66vd2mdu	1276	8	a	a	DT
cord-014597-66vd2mdu	1276	9	sequential	sequential	JJ
cord-014597-66vd2mdu	1276	10	adaptation	adaptation	NN
cord-014597-66vd2mdu	1276	11	protocol	protocol	NN
cord-014597-66vd2mdu	1276	12	of	of	IN
cord-014597-66vd2mdu	1276	13	our	-PRON-	PRP$
cord-014597-66vd2mdu	1276	14	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1276	15	RV	RV	NNP
cord-014597-66vd2mdu	1276	16	-	-	HYPH
cord-014597-66vd2mdu	1276	17	VLPs	vlps	NN
cord-014597-66vd2mdu	1276	18	producer	producer	NN
cord-014597-66vd2mdu	1276	19	clone	clone	NN
cord-014597-66vd2mdu	1276	20	,	,	,
cord-014597-66vd2mdu	1276	21	from	from	IN
cord-014597-66vd2mdu	1276	22	100	100	CD
cord-014597-66vd2mdu	1276	23	%	%	NN
cord-014597-66vd2mdu	1276	24	of	of	IN
cord-014597-66vd2mdu	1276	25	the	the	DT
cord-014597-66vd2mdu	1276	26	commercial	commercial	JJ
cord-014597-66vd2mdu	1276	27	SFM	SFM	NNP
cord-014597-66vd2mdu	1276	28	(	(	-LRB-
cord-014597-66vd2mdu	1276	29	EX	EX	NNP
cord-014597-66vd2mdu	1276	30	-	-	HYPH
cord-014597-66vd2mdu	1276	31	CELL293	CELL293	NNP
cord-014597-66vd2mdu	1276	32	,	,	,
cord-014597-66vd2mdu	1276	33	SAFC	SAFC	NNP
cord-014597-66vd2mdu	1276	34	)	)	-RRB-
cord-014597-66vd2mdu	1276	35	to	to	IN
cord-014597-66vd2mdu	1276	36	a	a	DT
cord-014597-66vd2mdu	1276	37	new	new	JJ
cord-014597-66vd2mdu	1276	38	formulation	formulation	NN
cord-014597-66vd2mdu	1276	39	with	with	IN
cord-014597-66vd2mdu	1276	40	only	only	RB
cord-014597-66vd2mdu	1276	41	50	50	CD
cord-014597-66vd2mdu	1276	42	%	%	NN
cord-014597-66vd2mdu	1276	43	of	of	IN
cord-014597-66vd2mdu	1276	44	the	the	DT
cord-014597-66vd2mdu	1276	45	SFM	SFM	NNP
cord-014597-66vd2mdu	1276	46	and	and	CC
cord-014597-66vd2mdu	1276	47	a	a	DT
cord-014597-66vd2mdu	1276	48	minimum	minimum	JJ
cord-014597-66vd2mdu	1276	49	essential	essential	JJ
cord-014597-66vd2mdu	1276	50	medium	medium	NN
cord-014597-66vd2mdu	1276	51	(	(	-LRB-
cord-014597-66vd2mdu	1276	52	P2	P2	NNP
cord-014597-66vd2mdu	1276	53	G	G	NNP
cord-014597-66vd2mdu	1276	54	)	)	-RRB-
cord-014597-66vd2mdu	1276	55	,	,	,
cord-014597-66vd2mdu	1276	56	developed	develop	VBN
cord-014597-66vd2mdu	1276	57	in	in	IN
cord-014597-66vd2mdu	1276	58	our	-PRON-	PRP$
cord-014597-66vd2mdu	1276	59	laboratory	laboratory	NN
cord-014597-66vd2mdu	1276	60	specifically	specifically	RB
cord-014597-66vd2mdu	1276	61	for	for	IN
cord-014597-66vd2mdu	1276	62	RV	rv	NN
cord-014597-66vd2mdu	1276	63	-	-	HYPH
cord-014597-66vd2mdu	1276	64	VLPs	vlp	NNS
cord-014597-66vd2mdu	1276	65	production	production	NN
cord-014597-66vd2mdu	1276	66	.	.	.
cord-014597-66vd2mdu	1277	1	This	this	DT
cord-014597-66vd2mdu	1277	2	new	new	JJ
cord-014597-66vd2mdu	1277	3	formulation	formulation	NN
cord-014597-66vd2mdu	1277	4	was	be	VBD
cord-014597-66vd2mdu	1277	5	called	call	VBN
cord-014597-66vd2mdu	1277	6	RVPM	RVPM	NNP
cord-014597-66vd2mdu	1278	1	(	(	-LRB-
cord-014597-66vd2mdu	1278	2	Rabies	Rabies	NNP
cord-014597-66vd2mdu	1278	3	Vaccine	Vaccine	NNP
cord-014597-66vd2mdu	1278	4	Production	Production	NNP
cord-014597-66vd2mdu	1278	5	Medium	Medium	NNP
cord-014597-66vd2mdu	1278	6	)	)	-RRB-
cord-014597-66vd2mdu	1278	7	.	.	.
cord-014597-66vd2mdu	1279	1	The	the	DT
cord-014597-66vd2mdu	1279	2	specific	specific	JJ
cord-014597-66vd2mdu	1279	3	productivity	productivity	NN
cord-014597-66vd2mdu	1279	4	of	of	IN
cord-014597-66vd2mdu	1279	5	RV	rv	NN
cord-014597-66vd2mdu	1279	6	-	-	HYPH
cord-014597-66vd2mdu	1279	7	VLPs	vlp	NNS
cord-014597-66vd2mdu	1279	8	in	in	IN
cord-014597-66vd2mdu	1279	9	culture	culture	NN
cord-014597-66vd2mdu	1279	10	supernatants	supernatant	NNS
cord-014597-66vd2mdu	1279	11	was	be	VBD
cord-014597-66vd2mdu	1279	12	measured	measure	VBN
cord-014597-66vd2mdu	1279	13	by	by	IN
cord-014597-66vd2mdu	1279	14	sandwich	sandwich	NN
cord-014597-66vd2mdu	1279	15	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1279	16	,	,	,
cord-014597-66vd2mdu	1279	17	using	use	VBG
cord-014597-66vd2mdu	1279	18	the	the	DT
cord-014597-66vd2mdu	1279	19	6	6	CD
cord-014597-66vd2mdu	1279	20	th	th	NNP
cord-014597-66vd2mdu	1279	21	International	International	NNP
cord-014597-66vd2mdu	1279	22	Standard	Standard	NNP
cord-014597-66vd2mdu	1279	23	for	for	IN
cord-014597-66vd2mdu	1279	24	rabies	rabies	NN
cord-014597-66vd2mdu	1279	25	vaccine	vaccine	NN
cord-014597-66vd2mdu	1279	26	that	that	WDT
cord-014597-66vd2mdu	1279	27	quantify	quantify	VBP
cord-014597-66vd2mdu	1279	28	the	the	DT
cord-014597-66vd2mdu	1279	29	glycoprotein	glycoprotein	NN
cord-014597-66vd2mdu	1279	30	content	content	NN
cord-014597-66vd2mdu	1279	31	(	(	-LRB-
cord-014597-66vd2mdu	1279	32	NIBSC	NIBSC	NNP
cord-014597-66vd2mdu	1279	33	,	,	,
cord-014597-66vd2mdu	1279	34	expressed	express	VBN
cord-014597-66vd2mdu	1279	35	in	in	IN
cord-014597-66vd2mdu	1279	36	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1279	37	Units	Units	NNP
cord-014597-66vd2mdu	1279	38	per	per	IN
cord-014597-66vd2mdu	1279	39	ml	ml	NN
cord-014597-66vd2mdu	1279	40	)	)	-RRB-
cord-014597-66vd2mdu	1279	41	.	.	.
cord-014597-66vd2mdu	1280	1	Further	further	RB
cord-014597-66vd2mdu	1280	2	,	,	,
cord-014597-66vd2mdu	1280	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1280	4	evaluated	evaluate	VBD
cord-014597-66vd2mdu	1280	5	both	both	DT
cord-014597-66vd2mdu	1280	6	media	medium	NNS
cord-014597-66vd2mdu	1280	7	for	for	IN
cord-014597-66vd2mdu	1280	8	the	the	DT
cord-014597-66vd2mdu	1280	9	production	production	NN
cord-014597-66vd2mdu	1280	10	of	of	IN
cord-014597-66vd2mdu	1280	11	the	the	DT
cord-014597-66vd2mdu	1280	12	rabies	rabies	NN
cord-014597-66vd2mdu	1280	13	vaccine	vaccine	NN
cord-014597-66vd2mdu	1280	14	,	,	,
cord-014597-66vd2mdu	1280	15	using	use	VBG
cord-014597-66vd2mdu	1280	16	stirred	stir	VBN
cord-014597-66vd2mdu	1280	17	tank	tank	NN
cord-014597-66vd2mdu	1280	18	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	1280	19	operated	operate	VBN
cord-014597-66vd2mdu	1280	20	in	in	IN
cord-014597-66vd2mdu	1280	21	continuous	continuous	JJ
cord-014597-66vd2mdu	1280	22	mode	mode	NN
cord-014597-66vd2mdu	1280	23	(	(	-LRB-
cord-014597-66vd2mdu	1280	24	BIOSTAT	BIOSTAT	NNP
cord-014597-66vd2mdu	1280	25	QPlus	QPlus	NNP
cord-014597-66vd2mdu	1280	26	,	,	,
cord-014597-66vd2mdu	1280	27	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	1280	28	)	)	-RRB-
cord-014597-66vd2mdu	1280	29	.	.	.
cord-014597-66vd2mdu	1281	1	The	the	DT
cord-014597-66vd2mdu	1281	2	production	production	NN
cord-014597-66vd2mdu	1281	3	of	of	IN
cord-014597-66vd2mdu	1281	4	the	the	DT
cord-014597-66vd2mdu	1281	5	RV	RV	NNP
cord-014597-66vd2mdu	1281	6	-	-	HYPH
cord-014597-66vd2mdu	1281	7	VLPs	VLPs	NNP
cord-014597-66vd2mdu	1281	8	was	be	VBD
cord-014597-66vd2mdu	1281	9	daily	daily	RB
cord-014597-66vd2mdu	1281	10	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1281	11	by	by	IN
cord-014597-66vd2mdu	1281	12	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1281	13	and	and	CC
cord-014597-66vd2mdu	1281	14	the	the	DT
cord-014597-66vd2mdu	1281	15	obtained	obtain	VBN
cord-014597-66vd2mdu	1281	16	harvests	harvest	NNS
cord-014597-66vd2mdu	1281	17	analysed	analyse	VBN
cord-014597-66vd2mdu	1281	18	by	by	IN
cord-014597-66vd2mdu	1281	19	the	the	DT
cord-014597-66vd2mdu	1281	20	NIH	NIH	NNP
cord-014597-66vd2mdu	1281	21	potency	potency	NN
cord-014597-66vd2mdu	1281	22	test	test	NN
cord-014597-66vd2mdu	1281	23	for	for	IN
cord-014597-66vd2mdu	1281	24	rabies	rabies	NN
cord-014597-66vd2mdu	1281	25	vaccine	vaccine	NN
cord-014597-66vd2mdu	1281	26	.	.	.
cord-014597-66vd2mdu	1282	1	After	after	IN
cord-014597-66vd2mdu	1282	2	the	the	DT
cord-014597-66vd2mdu	1282	3	adaptation	adaptation	NN
cord-014597-66vd2mdu	1282	4	process	process	NN
cord-014597-66vd2mdu	1282	5	,	,	,
cord-014597-66vd2mdu	1282	6	suspension	suspension	NN
cord-014597-66vd2mdu	1282	7	cultures	culture	NNS
cord-014597-66vd2mdu	1282	8	without	without	IN
cord-014597-66vd2mdu	1282	9	aggregates	aggregate	NNS
cord-014597-66vd2mdu	1282	10	or	or	CC
cord-014597-66vd2mdu	1282	11	clumps	clump	NNS
cord-014597-66vd2mdu	1282	12	were	be	VBD
cord-014597-66vd2mdu	1282	13	obtained	obtain	VBN
cord-014597-66vd2mdu	1282	14	,	,	,
cord-014597-66vd2mdu	1282	15	with	with	IN
cord-014597-66vd2mdu	1282	16	the	the	DT
cord-014597-66vd2mdu	1282	17	same	same	JJ
cord-014597-66vd2mdu	1282	18	specific	specific	JJ
cord-014597-66vd2mdu	1282	19	growth	growth	NN
cord-014597-66vd2mdu	1282	20	rate	rate	NN
cord-014597-66vd2mdu	1282	21	.	.	.
cord-014597-66vd2mdu	1283	1	A	a	DT
cord-014597-66vd2mdu	1283	2	lower	low	JJR
cord-014597-66vd2mdu	1283	3	maximum	maximum	JJ
cord-014597-66vd2mdu	1283	4	cell	cell	NN
cord-014597-66vd2mdu	1283	5	density	density	NN
cord-014597-66vd2mdu	1283	6	with	with	IN
cord-014597-66vd2mdu	1283	7	the	the	DT
cord-014597-66vd2mdu	1283	8	RVPM	RVPM	NNP
cord-014597-66vd2mdu	1283	9	was	be	VBD
cord-014597-66vd2mdu	1283	10	reached	reach	VBN
cord-014597-66vd2mdu	1283	11	,	,	,
cord-014597-66vd2mdu	1283	12	achieving	achieve	VBG
cord-014597-66vd2mdu	1283	13	5x10	5x10	CD
cord-014597-66vd2mdu	1283	14	6	6	CD
cord-014597-66vd2mdu	1283	15	cells.ml	cells.ml	CD
cord-014597-66vd2mdu	1283	16	-1	-1	NN
cord-014597-66vd2mdu	1283	17	,	,	,
cord-014597-66vd2mdu	1283	18	compared	compare	VBN
cord-014597-66vd2mdu	1283	19	with	with	IN
cord-014597-66vd2mdu	1283	20	the	the	DT
cord-014597-66vd2mdu	1283	21	SFM	SFM	NNP
cord-014597-66vd2mdu	1283	22	that	that	WDT
cord-014597-66vd2mdu	1283	23	reach	reach	VBP
cord-014597-66vd2mdu	1283	24	cell	cell	NN
cord-014597-66vd2mdu	1283	25	densities	density	NNS
cord-014597-66vd2mdu	1283	26	between	between	IN
cord-014597-66vd2mdu	1283	27	8	8	CD
cord-014597-66vd2mdu	1283	28	and	and	CC
cord-014597-66vd2mdu	1283	29	9x10	9x10	CD
cord-014597-66vd2mdu	1283	30	6	6	CD
cord-014597-66vd2mdu	1283	31	cells.ml	cells.ml	CD
cord-014597-66vd2mdu	1283	32	-1	-1	CD
cord-014597-66vd2mdu	1283	33	in	in	IN
cord-014597-66vd2mdu	1283	34	batch	batch	NN
cord-014597-66vd2mdu	1283	35	mode	mode	NN
cord-014597-66vd2mdu	1283	36	.	.	.
cord-014597-66vd2mdu	1284	1	The	the	DT
cord-014597-66vd2mdu	1284	2	specific	specific	JJ
cord-014597-66vd2mdu	1284	3	RV	rv	NN
cord-014597-66vd2mdu	1284	4	-	-	HYPH
cord-014597-66vd2mdu	1284	5	VLPs	vlps	NN
cord-014597-66vd2mdu	1284	6	productivity	productivity	NN
cord-014597-66vd2mdu	1284	7	per	per	IN
cord-014597-66vd2mdu	1284	8	cell	cell	NN
cord-014597-66vd2mdu	1284	9	was	be	VBD
cord-014597-66vd2mdu	1284	10	maintained	maintain	VBN
cord-014597-66vd2mdu	1284	11	,	,	,
cord-014597-66vd2mdu	1284	12	obtaining	obtain	VBG
cord-014597-66vd2mdu	1284	13	values	value	NNS
cord-014597-66vd2mdu	1284	14	of	of	IN
cord-014597-66vd2mdu	1284	15	0.88	0.88	CD
cord-014597-66vd2mdu	1284	16	and	and	CC
cord-014597-66vd2mdu	1284	17	0.90	0.90	CD
cord-014597-66vd2mdu	1284	18	EU.10	EU.10	NNS
cord-014597-66vd2mdu	1284	19	6	6	CD
cord-014597-66vd2mdu	1284	20	cells	cell	NNS
cord-014597-66vd2mdu	1284	21	-1	-1	.
cord-014597-66vd2mdu	1284	22	.day	.day	.
cord-014597-66vd2mdu	1285	1	-1	-1	.
cord-014597-66vd2mdu	1286	1	for	for	IN
cord-014597-66vd2mdu	1286	2	the	the	DT
cord-014597-66vd2mdu	1286	3	clone	clone	NN
cord-014597-66vd2mdu	1286	4	being	be	VBG
cord-014597-66vd2mdu	1286	5	cultured	culture	VBN
cord-014597-66vd2mdu	1286	6	in	in	IN
cord-014597-66vd2mdu	1286	7	SFM	SFM	NNP
cord-014597-66vd2mdu	1286	8	and	and	CC
cord-014597-66vd2mdu	1286	9	RVPM	RVPM	NNP
cord-014597-66vd2mdu	1286	10	,	,	,
cord-014597-66vd2mdu	1286	11	respectively	respectively	RB
cord-014597-66vd2mdu	1286	12	.	.	.
cord-014597-66vd2mdu	1287	1	Taking	take	VBG
cord-014597-66vd2mdu	1287	2	into	into	IN
cord-014597-66vd2mdu	1287	3	account	account	NN
cord-014597-66vd2mdu	1287	4	that	that	IN
cord-014597-66vd2mdu	1287	5	this	this	DT
cord-014597-66vd2mdu	1287	6	producer	producer	NN
cord-014597-66vd2mdu	1287	7	clone	clone	NN
cord-014597-66vd2mdu	1287	8	can	can	MD
cord-014597-66vd2mdu	1287	9	be	be	VB
cord-014597-66vd2mdu	1287	10	changed	change	VBN
cord-014597-66vd2mdu	1287	11	directly	directly	RB
cord-014597-66vd2mdu	1287	12	from	from	IN
cord-014597-66vd2mdu	1287	13	one	one	CD
cord-014597-66vd2mdu	1287	14	medium	medium	NN
cord-014597-66vd2mdu	1287	15	to	to	IN
cord-014597-66vd2mdu	1287	16	the	the	DT
cord-014597-66vd2mdu	1287	17	other	other	JJ
cord-014597-66vd2mdu	1287	18	without	without	IN
cord-014597-66vd2mdu	1287	19	lag	lag	NN
cord-014597-66vd2mdu	1287	20	phase	phase	NN
cord-014597-66vd2mdu	1287	21	or	or	CC
cord-014597-66vd2mdu	1287	22	cell	cell	NN
cord-014597-66vd2mdu	1287	23	damage	damage	NN
cord-014597-66vd2mdu	1287	24	,	,	,
cord-014597-66vd2mdu	1287	25	and	and	CC
cord-014597-66vd2mdu	1287	26	that	that	IN
cord-014597-66vd2mdu	1287	27	in	in	IN
cord-014597-66vd2mdu	1287	28	RVPM	RVPM	NNP
cord-014597-66vd2mdu	1287	29	the	the	DT
cord-014597-66vd2mdu	1287	30	maximum	maximum	JJ
cord-014597-66vd2mdu	1287	31	cell	cell	NN
cord-014597-66vd2mdu	1287	32	density	density	NN
cord-014597-66vd2mdu	1287	33	reached	reach	VBN
cord-014597-66vd2mdu	1287	34	was	be	VBD
cord-014597-66vd2mdu	1287	35	lower	low	JJR
cord-014597-66vd2mdu	1287	36	,	,	,
cord-014597-66vd2mdu	1287	37	this	this	DT
cord-014597-66vd2mdu	1287	38	medium	medium	NN
cord-014597-66vd2mdu	1287	39	was	be	VBD
cord-014597-66vd2mdu	1287	40	proposed	propose	VBN
cord-014597-66vd2mdu	1287	41	to	to	TO
cord-014597-66vd2mdu	1287	42	be	be	VB
cord-014597-66vd2mdu	1287	43	analysed	analyse	VBN
cord-014597-66vd2mdu	1287	44	in	in	IN
cord-014597-66vd2mdu	1287	45	high	high	JJ
cord-014597-66vd2mdu	1287	46	cell	cell	NN
cord-014597-66vd2mdu	1287	47	density	density	NN
cord-014597-66vd2mdu	1287	48	in	in	IN
cord-014597-66vd2mdu	1287	49	perfusion	perfusion	NN
cord-014597-66vd2mdu	1287	50	mode	mode	NN
cord-014597-66vd2mdu	1287	51	for	for	IN
cord-014597-66vd2mdu	1287	52	a	a	DT
cord-014597-66vd2mdu	1287	53	continuous	continuous	JJ
cord-014597-66vd2mdu	1287	54	culture	culture	NN
cord-014597-66vd2mdu	1287	55	in	in	IN
cord-014597-66vd2mdu	1287	56	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1287	57	.	.	.
cord-014597-66vd2mdu	1288	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1288	2	,	,	,
cord-014597-66vd2mdu	1288	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1288	4	performed	perform	VBD
cord-014597-66vd2mdu	1288	5	two	two	CD
cord-014597-66vd2mdu	1288	6	cultures	culture	NNS
cord-014597-66vd2mdu	1288	7	in	in	IN
cord-014597-66vd2mdu	1288	8	parallel	parallel	NN
cord-014597-66vd2mdu	1288	9	to	to	TO
cord-014597-66vd2mdu	1288	10	compare	compare	VB
cord-014597-66vd2mdu	1288	11	the	the	DT
cord-014597-66vd2mdu	1288	12	efficacy	efficacy	NN
cord-014597-66vd2mdu	1288	13	of	of	IN
cord-014597-66vd2mdu	1288	14	each	each	DT
cord-014597-66vd2mdu	1288	15	media	medium	NNS
cord-014597-66vd2mdu	1288	16	formulation	formulation	NN
cord-014597-66vd2mdu	1288	17	in	in	IN
cord-014597-66vd2mdu	1288	18	perfusion	perfusion	NN
cord-014597-66vd2mdu	1288	19	.	.	.
cord-014597-66vd2mdu	1289	1	As	as	IN
cord-014597-66vd2mdu	1289	2	shown	show	VBN
cord-014597-66vd2mdu	1289	3	in	in	IN
cord-014597-66vd2mdu	1289	4	Fig	Fig	NNP
cord-014597-66vd2mdu	1289	5	.	.	.
cord-014597-66vd2mdu	1290	1	1	1	LS
cord-014597-66vd2mdu	1290	2	,	,	,
cord-014597-66vd2mdu	1290	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1290	4	obtained	obtain	VBD
cord-014597-66vd2mdu	1290	5	very	very	RB
cord-014597-66vd2mdu	1290	6	similar	similar	JJ
cord-014597-66vd2mdu	1290	7	culture	culture	NN
cord-014597-66vd2mdu	1290	8	performances	performance	NNS
cord-014597-66vd2mdu	1290	9	in	in	IN
cord-014597-66vd2mdu	1290	10	both	both	DT
cord-014597-66vd2mdu	1290	11	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	1290	12	;	;	:
cord-014597-66vd2mdu	1290	13	14.4	14.4	CD
cord-014597-66vd2mdu	1290	14	EU.ml	eu.ml	NN
cord-014597-66vd2mdu	1290	15	-1	-1	NN
cord-014597-66vd2mdu	1290	16	and	and	CC
cord-014597-66vd2mdu	1290	17	16.1	16.1	CD
cord-014597-66vd2mdu	1290	18	EU.ml	EU.ml	NNP
cord-014597-66vd2mdu	1290	19	-1	-1	NN
cord-014597-66vd2mdu	1290	20	of	of	IN
cord-014597-66vd2mdu	1290	21	RV	rv	NN
cord-014597-66vd2mdu	1290	22	-	-	HYPH
cord-014597-66vd2mdu	1290	23	VLPs	vlp	NNS
cord-014597-66vd2mdu	1290	24	for	for	IN
cord-014597-66vd2mdu	1290	25	the	the	DT
cord-014597-66vd2mdu	1290	26	commercial	commercial	JJ
cord-014597-66vd2mdu	1290	27	SFM	SFM	NNP
cord-014597-66vd2mdu	1290	28	and	and	CC
cord-014597-66vd2mdu	1290	29	RVPM	RVPM	NNP
cord-014597-66vd2mdu	1290	30	,	,	,
cord-014597-66vd2mdu	1290	31	respectively	respectively	RB
cord-014597-66vd2mdu	1290	32	.	.	.
cord-014597-66vd2mdu	1291	1	After	after	IN
cord-014597-66vd2mdu	1291	2	that	that	DT
cord-014597-66vd2mdu	1291	3	,	,	,
cord-014597-66vd2mdu	1291	4	the	the	DT
cord-014597-66vd2mdu	1291	5	harvests	harvest	NNS
cord-014597-66vd2mdu	1291	6	were	be	VBD
cord-014597-66vd2mdu	1291	7	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1291	8	by	by	IN
cord-014597-66vd2mdu	1291	9	the	the	DT
cord-014597-66vd2mdu	1291	10	NIH	NIH	NNP
cord-014597-66vd2mdu	1291	11	potency	potency	NN
cord-014597-66vd2mdu	1291	12	test	test	NN
cord-014597-66vd2mdu	1291	13	obtaining	obtain	VBG
cord-014597-66vd2mdu	1291	14	a	a	DT
cord-014597-66vd2mdu	1291	15	rabies	rabies	NN
cord-014597-66vd2mdu	1291	16	vaccine	vaccine	NN
cord-014597-66vd2mdu	1291	17	potency	potency	NN
cord-014597-66vd2mdu	1291	18	of	of	IN
cord-014597-66vd2mdu	1291	19	1.2	1.2	CD
cord-014597-66vd2mdu	1291	20	IU.ml	IU.ml	NNP
cord-014597-66vd2mdu	1291	21	-1	-1	.
cord-014597-66vd2mdu	1292	1	for	for	IN
cord-014597-66vd2mdu	1292	2	both	both	DT
cord-014597-66vd2mdu	1292	3	cultures	culture	NNS
cord-014597-66vd2mdu	1292	4	(	(	-LRB-
cord-014597-66vd2mdu	1292	5	being	be	VBG
cord-014597-66vd2mdu	1292	6	1	1	CD
cord-014597-66vd2mdu	1293	1	IU.ml	IU.ml	NNP
cord-014597-66vd2mdu	1293	2	-1	-1	.
cord-014597-66vd2mdu	1294	1	the	the	DT
cord-014597-66vd2mdu	1294	2	minimum	minimum	JJ
cord-014597-66vd2mdu	1294	3	potency	potency	NN
cord-014597-66vd2mdu	1294	4	required	require	VBN
cord-014597-66vd2mdu	1294	5	for	for	IN
cord-014597-66vd2mdu	1294	6	animal	animal	NN
cord-014597-66vd2mdu	1294	7	vaccine	vaccine	NN
cord-014597-66vd2mdu	1294	8	)	)	-RRB-
cord-014597-66vd2mdu	1294	9	.	.	.
cord-014597-66vd2mdu	1295	1	Thus	thus	RB
cord-014597-66vd2mdu	1295	2	,	,	,
cord-014597-66vd2mdu	1295	3	the	the	DT
cord-014597-66vd2mdu	1295	4	results	result	NNS
cord-014597-66vd2mdu	1295	5	obtained	obtain	VBD
cord-014597-66vd2mdu	1295	6	represent	represent	VBP
cord-014597-66vd2mdu	1295	7	an	an	DT
cord-014597-66vd2mdu	1295	8	interesting	interesting	JJ
cord-014597-66vd2mdu	1295	9	advance	advance	NN
cord-014597-66vd2mdu	1295	10	in	in	IN
cord-014597-66vd2mdu	1295	11	the	the	DT
cord-014597-66vd2mdu	1295	12	optimization	optimization	NN
cord-014597-66vd2mdu	1295	13	of	of	IN
cord-014597-66vd2mdu	1295	14	this	this	DT
cord-014597-66vd2mdu	1295	15	vaccine	vaccine	NN
cord-014597-66vd2mdu	1295	16	production	production	NN
cord-014597-66vd2mdu	1295	17	process	process	NN
cord-014597-66vd2mdu	1295	18	since	since	IN
cord-014597-66vd2mdu	1295	19	the	the	DT
cord-014597-66vd2mdu	1295	20	use	use	NN
cord-014597-66vd2mdu	1295	21	of	of	IN
cord-014597-66vd2mdu	1295	22	this	this	DT
cord-014597-66vd2mdu	1295	23	new	new	JJ
cord-014597-66vd2mdu	1295	24	medium	medium	JJ
cord-014597-66vd2mdu	1295	25	formulation	formulation	NN
cord-014597-66vd2mdu	1295	26	represents	represent	VBZ
cord-014597-66vd2mdu	1295	27	a	a	DT
cord-014597-66vd2mdu	1295	28	reduction	reduction	NN
cord-014597-66vd2mdu	1295	29	of	of	IN
cord-014597-66vd2mdu	1295	30	40	40	CD
cord-014597-66vd2mdu	1295	31	%	%	NN
cord-014597-66vd2mdu	1295	32	of	of	IN
cord-014597-66vd2mdu	1295	33	the	the	DT
cord-014597-66vd2mdu	1295	34	total	total	JJ
cord-014597-66vd2mdu	1295	35	cost	cost	NN
cord-014597-66vd2mdu	1295	36	which	which	WDT
cord-014597-66vd2mdu	1295	37	will	will	MD
cord-014597-66vd2mdu	1295	38	be	be	VB
cord-014597-66vd2mdu	1295	39	reflected	reflect	VBN
cord-014597-66vd2mdu	1295	40	in	in	IN
cord-014597-66vd2mdu	1295	41	a	a	DT
cord-014597-66vd2mdu	1295	42	considerable	considerable	JJ
cord-014597-66vd2mdu	1295	43	reduction	reduction	NN
cord-014597-66vd2mdu	1295	44	of	of	IN
cord-014597-66vd2mdu	1295	45	the	the	DT
cord-014597-66vd2mdu	1295	46	price	price	NN
cord-014597-66vd2mdu	1295	47	of	of	IN
cord-014597-66vd2mdu	1295	48	the	the	DT
cord-014597-66vd2mdu	1295	49	vaccine	vaccine	NN
cord-014597-66vd2mdu	1295	50	dose	dose	NN
cord-014597-66vd2mdu	1295	51	.	.	.
cord-014597-66vd2mdu	1296	1	Background	background	NN
cord-014597-66vd2mdu	1296	2	Vaccines	vaccine	NNS
cord-014597-66vd2mdu	1296	3	are	be	VBP
cord-014597-66vd2mdu	1296	4	one	one	CD
cord-014597-66vd2mdu	1296	5	of	of	IN
cord-014597-66vd2mdu	1296	6	the	the	DT
cord-014597-66vd2mdu	1296	7	most	most	RBS
cord-014597-66vd2mdu	1296	8	powerful	powerful	JJ
cord-014597-66vd2mdu	1296	9	and	and	CC
cord-014597-66vd2mdu	1296	10	effective	effective	JJ
cord-014597-66vd2mdu	1296	11	health	health	NN
cord-014597-66vd2mdu	1296	12	inventions	invention	NNS
cord-014597-66vd2mdu	1296	13	ever	ever	RB
cord-014597-66vd2mdu	1296	14	developedproviding	developedprovide	VBG
cord-014597-66vd2mdu	1296	15	tremendous	tremendous	JJ
cord-014597-66vd2mdu	1296	16	economic	economic	JJ
cord-014597-66vd2mdu	1296	17	and	and	CC
cord-014597-66vd2mdu	1296	18	societal	societal	JJ
cord-014597-66vd2mdu	1296	19	value	value	NN
cord-014597-66vd2mdu	1296	20	;	;	:
cord-014597-66vd2mdu	1296	21	yet	yet	CC
cord-014597-66vd2mdu	1296	22	several	several	JJ
cord-014597-66vd2mdu	1296	23	factors	factor	NNS
cord-014597-66vd2mdu	1296	24	hinder	hinder	VBP
cord-014597-66vd2mdu	1296	25	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	1296	26	immunization	immunization	NN
cord-014597-66vd2mdu	1296	27	coverage	coverage	NN
cord-014597-66vd2mdu	1296	28	.	.	.
cord-014597-66vd2mdu	1297	1	Traditional	traditional	JJ
cord-014597-66vd2mdu	1297	2	methods	method	NNS
cord-014597-66vd2mdu	1297	3	of	of	IN
cord-014597-66vd2mdu	1297	4	biologics	biologic	NNS
cord-014597-66vd2mdu	1297	5	production	production	NN
cord-014597-66vd2mdu	1297	6	,	,	,
cord-014597-66vd2mdu	1297	7	based	base	VBN
cord-014597-66vd2mdu	1297	8	on	on	IN
cord-014597-66vd2mdu	1297	9	stainless	stainless	JJ
cord-014597-66vd2mdu	1297	10	steel	steel	NN
cord-014597-66vd2mdu	1297	11	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	1297	12	,	,	,
cord-014597-66vd2mdu	1297	13	allow	allow	VB
cord-014597-66vd2mdu	1297	14	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	1297	15	companies	company	NNS
cord-014597-66vd2mdu	1297	16	to	to	TO
cord-014597-66vd2mdu	1297	17	achieve	achieve	VB
cord-014597-66vd2mdu	1297	18	economy	economy	NN
cord-014597-66vd2mdu	1297	19	of	of	IN
cord-014597-66vd2mdu	1297	20	scale	scale	NN
cord-014597-66vd2mdu	1297	21	,	,	,
cord-014597-66vd2mdu	1297	22	but	but	CC
cord-014597-66vd2mdu	1297	23	are	be	VBP
cord-014597-66vd2mdu	1297	24	limited	limit	VBN
cord-014597-66vd2mdu	1297	25	by	by	IN
cord-014597-66vd2mdu	1297	26	high	high	JJ
cord-014597-66vd2mdu	1297	27	capital	capital	NN
cord-014597-66vd2mdu	1297	28	expenditures	expenditure	NNS
cord-014597-66vd2mdu	1297	29	.	.	.
cord-014597-66vd2mdu	1298	1	Such	such	JJ
cord-014597-66vd2mdu	1298	2	approaches	approach	NNS
cord-014597-66vd2mdu	1298	3	stifle	stifle	VBP
cord-014597-66vd2mdu	1298	4	manufacturing	manufacture	VBG
cord-014597-66vd2mdu	1298	5	innovation	innovation	NN
cord-014597-66vd2mdu	1298	6	and	and	CC
cord-014597-66vd2mdu	1298	7	lack	lack	JJ
cord-014597-66vd2mdu	1298	8	long	long	JJ
cord-014597-66vd2mdu	1298	9	-	-	HYPH
cord-014597-66vd2mdu	1298	10	term	term	NN
cord-014597-66vd2mdu	1298	11	cost	cost	NN
cord-014597-66vd2mdu	1298	12	-	-	HYPH
cord-014597-66vd2mdu	1298	13	effectiveness	effectiveness	NN
cord-014597-66vd2mdu	1298	14	and	and	CC
cord-014597-66vd2mdu	1298	15	sustainability	sustainability	NN
cord-014597-66vd2mdu	1298	16	.	.	.
cord-014597-66vd2mdu	1299	1	Current	current	JJ
cord-014597-66vd2mdu	1299	2	innovations	innovation	NNS
cord-014597-66vd2mdu	1299	3	can	can	MD
cord-014597-66vd2mdu	1299	4	cut	cut	VB
cord-014597-66vd2mdu	1299	5	biologics	biologic	NNS
cord-014597-66vd2mdu	1299	6	'	'	POS
cord-014597-66vd2mdu	1299	7	production	production	NN
cord-014597-66vd2mdu	1299	8	costs	cost	NNS
cord-014597-66vd2mdu	1299	9	to	to	TO
cord-014597-66vd2mdu	1299	10	revolutionize	revolutionize	VB
cord-014597-66vd2mdu	1299	11	the	the	DT
cord-014597-66vd2mdu	1299	12	mainstream	mainstream	NN
cord-014597-66vd2mdu	1299	13	use	use	NN
cord-014597-66vd2mdu	1299	14	of	of	IN
cord-014597-66vd2mdu	1299	15	biologic	biologic	JJ
cord-014597-66vd2mdu	1299	16	treatments	treatment	NNS
cord-014597-66vd2mdu	1299	17	,	,	,
cord-014597-66vd2mdu	1299	18	focusing	focus	VBG
cord-014597-66vd2mdu	1299	19	on	on	IN
cord-014597-66vd2mdu	1299	20	developing	develop	VBG
cord-014597-66vd2mdu	1299	21	fast	fast	RB
cord-014597-66vd2mdu	1299	22	,	,	,
cord-014597-66vd2mdu	1299	23	potent	potent	JJ
cord-014597-66vd2mdu	1299	24	and	and	CC
cord-014597-66vd2mdu	1299	25	cost	cost	NN
cord-014597-66vd2mdu	1299	26	-	-	HYPH
cord-014597-66vd2mdu	1299	27	effective	effective	JJ
cord-014597-66vd2mdu	1299	28	vaccine	vaccine	NN
cord-014597-66vd2mdu	1299	29	production	production	NN
cord-014597-66vd2mdu	1299	30	.	.	.
cord-014597-66vd2mdu	1300	1	Univercells	Univercells	NNP
cord-014597-66vd2mdu	1300	2	'	'	POS
cord-014597-66vd2mdu	1300	3	mission	mission	NN
cord-014597-66vd2mdu	1300	4	to	to	TO
cord-014597-66vd2mdu	1300	5	make	make	VB
cord-014597-66vd2mdu	1300	6	biologics	biologic	NNS
cord-014597-66vd2mdu	1300	7	affordable	affordable	JJ
cord-014597-66vd2mdu	1300	8	to	to	IN
cord-014597-66vd2mdu	1300	9	all	all	DT
cord-014597-66vd2mdu	1300	10	initiated	initiate	VBN
cord-014597-66vd2mdu	1300	11	a	a	DT
cord-014597-66vd2mdu	1300	12	paradigm	paradigm	NN
cord-014597-66vd2mdu	1300	13	shift	shift	NN
cord-014597-66vd2mdu	1300	14	,	,	,
cord-014597-66vd2mdu	1300	15	targeting	target	VBG
cord-014597-66vd2mdu	1300	16	an	an	DT
cord-014597-66vd2mdu	1300	17	innovative	innovative	JJ
cord-014597-66vd2mdu	1300	18	single	single	JJ
cord-014597-66vd2mdu	1300	19	-	-	HYPH
cord-014597-66vd2mdu	1300	20	use	use	NN
cord-014597-66vd2mdu	1300	21	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1300	22	platform	platform	NN
cord-014597-66vd2mdu	1300	23	incorporating	incorporate	VBG
cord-014597-66vd2mdu	1300	24	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	1300	25	into	into	IN
cord-014597-66vd2mdu	1300	26	continuous	continuous	JJ
cord-014597-66vd2mdu	1300	27	operations	operation	NNS
cord-014597-66vd2mdu	1300	28	.	.	.
cord-014597-66vd2mdu	1301	1	Univercells	Univercells	NNP
cord-014597-66vd2mdu	1301	2	employs	employ	VBZ
cord-014597-66vd2mdu	1301	3	process	process	NN
cord-014597-66vd2mdu	1301	4	intensification	intensification	NN
cord-014597-66vd2mdu	1301	5	,	,	,
cord-014597-66vd2mdu	1301	6	using	use	VBG
cord-014597-66vd2mdu	1301	7	high	high	JJ
cord-014597-66vd2mdu	1301	8	volumetric	volumetric	JJ
cord-014597-66vd2mdu	1301	9	productivity	productivity	NN
cord-014597-66vd2mdu	1301	10	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	1301	11	;	;	:
cord-014597-66vd2mdu	1301	12	and	and	CC
cord-014597-66vd2mdu	1301	13	unit	unit	NN
cord-014597-66vd2mdu	1301	14	steps	step	NNS
cord-014597-66vd2mdu	1301	15	integration	integration	NN
cord-014597-66vd2mdu	1301	16	,	,	,
cord-014597-66vd2mdu	1301	17	coupling	couple	VBG
cord-014597-66vd2mdu	1301	18	USP	USP	NNP
cord-014597-66vd2mdu	1301	19	and	and	CC
cord-014597-66vd2mdu	1301	20	DSP	DSP	NNP
cord-014597-66vd2mdu	1301	21	into	into	IN
cord-014597-66vd2mdu	1301	22	continuous	continuous	JJ
cord-014597-66vd2mdu	1301	23	operations	operation	NNS
cord-014597-66vd2mdu	1301	24	.	.	.
cord-014597-66vd2mdu	1302	1	The	the	DT
cord-014597-66vd2mdu	1302	2	objective	objective	NN
cord-014597-66vd2mdu	1302	3	is	be	VBZ
cord-014597-66vd2mdu	1302	4	a	a	DT
cord-014597-66vd2mdu	1302	5	down	down	RB
cord-014597-66vd2mdu	1302	6	-	-	HYPH
cord-014597-66vd2mdu	1302	7	scaled	scale	VBN
cord-014597-66vd2mdu	1302	8	high	high	JJ
cord-014597-66vd2mdu	1302	9	-	-	HYPH
cord-014597-66vd2mdu	1302	10	productivity	productivity	NN
cord-014597-66vd2mdu	1302	11	process	process	NN
cord-014597-66vd2mdu	1302	12	for	for	IN
cord-014597-66vd2mdu	1302	13	a	a	DT
cord-014597-66vd2mdu	1302	14	cost	cost	NN
cord-014597-66vd2mdu	1302	15	-	-	HYPH
cord-014597-66vd2mdu	1302	16	effective	effective	JJ
cord-014597-66vd2mdu	1302	17	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1302	18	solution	solution	NN
cord-014597-66vd2mdu	1302	19	.	.	.
cord-014597-66vd2mdu	1303	1	The	the	DT
cord-014597-66vd2mdu	1303	2	resulting	result	VBG
cord-014597-66vd2mdu	1303	3	micro	micro	NN
cord-014597-66vd2mdu	1303	4	-	-	NNS
cord-014597-66vd2mdu	1303	5	facilities	facility	NNS
cord-014597-66vd2mdu	1303	6	are	be	VBP
cord-014597-66vd2mdu	1303	7	easily	easily	RB
cord-014597-66vd2mdu	1303	8	-	-	HYPH
cord-014597-66vd2mdu	1303	9	deployable	deployable	JJ
cord-014597-66vd2mdu	1303	10	in	in	IN
cord-014597-66vd2mdu	1303	11	developing	develop	VBG
cord-014597-66vd2mdu	1303	12	countries	country	NNS
cord-014597-66vd2mdu	1303	13	,	,	,
cord-014597-66vd2mdu	1303	14	breaking	break	VBG
cord-014597-66vd2mdu	1303	15	entry	entry	NN
cord-014597-66vd2mdu	1303	16	barriers	barrier	NNS
cord-014597-66vd2mdu	1303	17	to	to	IN
cord-014597-66vd2mdu	1303	18	biomanufacturing	biomanufacture	VBG
cord-014597-66vd2mdu	1303	19	(	(	-LRB-
cord-014597-66vd2mdu	1303	20	Fig	Fig	NNP
cord-014597-66vd2mdu	1303	21	.	.	.
cord-014597-66vd2mdu	1303	22	1	1	CD
cord-014597-66vd2mdu	1303	23	)	)	-RRB-
cord-014597-66vd2mdu	1303	24	.	.	.
cord-014597-66vd2mdu	1304	1	Manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1304	2	and	and	CC
cord-014597-66vd2mdu	1304	3	distribution	distribution	NN
cord-014597-66vd2mdu	1304	4	advancements	advancement	NNS
cord-014597-66vd2mdu	1304	5	,	,	,
cord-014597-66vd2mdu	1304	6	from	from	IN
cord-014597-66vd2mdu	1304	7	centralized	centralize	VBN
cord-014597-66vd2mdu	1304	8	to	to	IN
cord-014597-66vd2mdu	1304	9	distributed	distribute	VBN
cord-014597-66vd2mdu	1304	10	,	,	,
cord-014597-66vd2mdu	1304	11	foresee	foresee	VB
cord-014597-66vd2mdu	1304	12	affordable	affordable	JJ
cord-014597-66vd2mdu	1304	13	treatments	treatment	NNS
cord-014597-66vd2mdu	1304	14	'	'	POS
cord-014597-66vd2mdu	1304	15	obtainability	obtainability	NN
cord-014597-66vd2mdu	1304	16	via	via	IN
cord-014597-66vd2mdu	1304	17	supplying	supply	VBG
cord-014597-66vd2mdu	1304	18	local	local	JJ
cord-014597-66vd2mdu	1304	19	populations	population	NNS
cord-014597-66vd2mdu	1304	20	with	with	IN
cord-014597-66vd2mdu	1304	21	local	local	JJ
cord-014597-66vd2mdu	1304	22	production	production	NN
cord-014597-66vd2mdu	1304	23	units	unit	NNS
cord-014597-66vd2mdu	1304	24	.	.	.
cord-014597-66vd2mdu	1305	1	-Bench	-bench	LS
cord-014597-66vd2mdu	1305	2	-	-	HYPH
cord-014597-66vd2mdu	1305	3	scale	scale	NN
cord-014597-66vd2mdu	1305	4	fixed	fix	VBN
cord-014597-66vd2mdu	1305	5	-	-	HYPH
cord-014597-66vd2mdu	1305	6	bed	bed	NN
cord-014597-66vd2mdu	1305	7	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1305	8	;	;	:
cord-014597-66vd2mdu	1305	9	-Carriers	-Carriers	:
cord-014597-66vd2mdu	1305	10	made	make	VBN
cord-014597-66vd2mdu	1305	11	of	of	IN
cord-014597-66vd2mdu	1305	12	100	100	CD
cord-014597-66vd2mdu	1305	13	%	%	NN
cord-014597-66vd2mdu	1305	14	pure	pure	JJ
cord-014597-66vd2mdu	1305	15	non	non	JJ
cord-014597-66vd2mdu	1305	16	-	-	JJ
cord-014597-66vd2mdu	1305	17	woven	woven	JJ
cord-014597-66vd2mdu	1305	18	hydrophilized	hydrophilized	JJ
cord-014597-66vd2mdu	1305	19	PET	pet	JJ
cord-014597-66vd2mdu	1305	20	fibers	fiber	NNS
cord-014597-66vd2mdu	1305	21	;	;	:
cord-014597-66vd2mdu	1305	22	-Vero	-vero	CD
cord-014597-66vd2mdu	1305	23	cells	cell	NNS
cord-014597-66vd2mdu	1305	24	grown	grow	VBN
cord-014597-66vd2mdu	1305	25	in	in	IN
cord-014597-66vd2mdu	1305	26	serum	serum	NN
cord-014597-66vd2mdu	1305	27	-	-	HYPH
cord-014597-66vd2mdu	1305	28	free	free	JJ
cord-014597-66vd2mdu	1305	29	and	and	CC
cord-014597-66vd2mdu	1305	30	serum	serum	NN
cord-014597-66vd2mdu	1305	31	containing	contain	VBG
cord-014597-66vd2mdu	1305	32	media	medium	NNS
cord-014597-66vd2mdu	1305	33	;	;	,
cord-014597-66vd2mdu	1305	34	-Attenuated	-attenuate	VBD
cord-014597-66vd2mdu	1305	35	polio	polio	NN
cord-014597-66vd2mdu	1305	36	strains	strain	NNS
cord-014597-66vd2mdu	1305	37	;	;	:
cord-014597-66vd2mdu	1306	1	-Cell	-Cell	NFP
cord-014597-66vd2mdu	1306	2	nuclei	nucleus	NNS
cord-014597-66vd2mdu	1306	3	on	on	IN
cord-014597-66vd2mdu	1306	4	carriers	carrier	NNS
cord-014597-66vd2mdu	1306	5	counted	count	VBN
cord-014597-66vd2mdu	1306	6	by	by	IN
cord-014597-66vd2mdu	1306	7	the	the	DT
cord-014597-66vd2mdu	1306	8	crystal	crystal	NN
cord-014597-66vd2mdu	1306	9	violet	violet	NN
cord-014597-66vd2mdu	1306	10	method	method	NN
cord-014597-66vd2mdu	1306	11	;	;	:
cord-014597-66vd2mdu	1306	12	-Polio	-polio	WDT
cord-014597-66vd2mdu	1306	13	virus	virus	NN
cord-014597-66vd2mdu	1306	14	production	production	NN
cord-014597-66vd2mdu	1306	15	estimated	estimate	VBN
cord-014597-66vd2mdu	1306	16	by	by	IN
cord-014597-66vd2mdu	1306	17	Elisa	Elisa	NNP
cord-014597-66vd2mdu	1306	18	assay	assay	NN
cord-014597-66vd2mdu	1306	19	(	(	-LRB-
cord-014597-66vd2mdu	1306	20	D	d	NN
cord-014597-66vd2mdu	1306	21	-	-	HYPH
cord-014597-66vd2mdu	1306	22	antigen	antigen	NN
cord-014597-66vd2mdu	1306	23	content	content	NN
cord-014597-66vd2mdu	1306	24	)	)	-RRB-
cord-014597-66vd2mdu	1306	25	.	.	.
cord-014597-66vd2mdu	1307	1	Cultivation	cultivation	NN
cord-014597-66vd2mdu	1307	2	of	of	IN
cord-014597-66vd2mdu	1307	3	Vero	Vero	NNP
cord-014597-66vd2mdu	1307	4	cells	cell	NNS
cord-014597-66vd2mdu	1307	5	in	in	IN
cord-014597-66vd2mdu	1307	6	medium	medium	NN
cord-014597-66vd2mdu	1307	7	with	with	IN
cord-014597-66vd2mdu	1307	8	serum	serum	NN
cord-014597-66vd2mdu	1307	9	and	and	CC
cord-014597-66vd2mdu	1307	10	in	in	IN
cord-014597-66vd2mdu	1307	11	serum	serum	NN
cord-014597-66vd2mdu	1307	12	-	-	HYPH
cord-014597-66vd2mdu	1307	13	free	free	JJ
cord-014597-66vd2mdu	1307	14	medium	medium	NN
cord-014597-66vd2mdu	1307	15	,	,	,
cord-014597-66vd2mdu	1307	16	was	be	VBD
cord-014597-66vd2mdu	1307	17	carried	carry	VBN
cord-014597-66vd2mdu	1307	18	out	out	RP
cord-014597-66vd2mdu	1307	19	in	in	IN
cord-014597-66vd2mdu	1307	20	bench	bench	NN
cord-014597-66vd2mdu	1307	21	-	-	HYPH
cord-014597-66vd2mdu	1307	22	scale	scale	NN
cord-014597-66vd2mdu	1307	23	compact	compact	JJ
cord-014597-66vd2mdu	1307	24	fixed	fix	VBN
cord-014597-66vd2mdu	1307	25	-	-	HYPH
cord-014597-66vd2mdu	1307	26	bed	bed	NN
cord-014597-66vd2mdu	1307	27	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	1307	28	,	,	,
cord-014597-66vd2mdu	1307	29	to	to	TO
cord-014597-66vd2mdu	1307	30	determine	determine	VB
cord-014597-66vd2mdu	1307	31	which	which	WDT
cord-014597-66vd2mdu	1307	32	culture	culture	NN
cord-014597-66vd2mdu	1307	33	conditions	condition	NNS
cord-014597-66vd2mdu	1307	34	result	result	VBP
cord-014597-66vd2mdu	1307	35	in	in	IN
cord-014597-66vd2mdu	1307	36	the	the	DT
cord-014597-66vd2mdu	1307	37	highest	high	JJS
cord-014597-66vd2mdu	1307	38	growth	growth	NN
cord-014597-66vd2mdu	1307	39	rate	rate	NN
cord-014597-66vd2mdu	1307	40	,	,	,
cord-014597-66vd2mdu	1307	41	the	the	DT
cord-014597-66vd2mdu	1307	42	highest	high	JJS
cord-014597-66vd2mdu	1307	43	cell	cell	NN
cord-014597-66vd2mdu	1307	44	biomass	biomass	NN
cord-014597-66vd2mdu	1307	45	by	by	IN
cord-014597-66vd2mdu	1307	46	carriers	carrier	NNS
cord-014597-66vd2mdu	1307	47	and	and	CC
cord-014597-66vd2mdu	1307	48	virus	virus	NN
cord-014597-66vd2mdu	1307	49	production	production	NN
cord-014597-66vd2mdu	1307	50	.	.	.
cord-014597-66vd2mdu	1308	1	Cells	cell	NNS
cord-014597-66vd2mdu	1308	2	were	be	VBD
cord-014597-66vd2mdu	1308	3	inoculated	inoculate	VBN
cord-014597-66vd2mdu	1308	4	at	at	IN
cord-014597-66vd2mdu	1308	5	0.05x10	0.05x10	CD
cord-014597-66vd2mdu	1308	6	6	6	CD
cord-014597-66vd2mdu	1308	7	cells	cell	NNS
cord-014597-66vd2mdu	1308	8	/	/	SYM
cord-014597-66vd2mdu	1308	9	cm	cm	NN
cord-014597-66vd2mdu	1308	10	2	2	CD
cord-014597-66vd2mdu	1308	11	and	and	CC
cord-014597-66vd2mdu	1308	12	infected	infect	VBN
cord-014597-66vd2mdu	1308	13	during	during	IN
cord-014597-66vd2mdu	1308	14	the	the	DT
cord-014597-66vd2mdu	1308	15	mid	mid	JJ
cord-014597-66vd2mdu	1308	16	-	-	JJ
cord-014597-66vd2mdu	1308	17	exponential	exponential	JJ
cord-014597-66vd2mdu	1308	18	phase	phase	NN
cord-014597-66vd2mdu	1308	19	,	,	,
cord-014597-66vd2mdu	1308	20	following	follow	VBG
cord-014597-66vd2mdu	1308	21	a	a	DT
cord-014597-66vd2mdu	1308	22	complete	complete	JJ
cord-014597-66vd2mdu	1308	23	media	media	NN
cord-014597-66vd2mdu	1308	24	exchange	exchange	NN
cord-014597-66vd2mdu	1308	25	.	.	.
cord-014597-66vd2mdu	1309	1	Viral	viral	JJ
cord-014597-66vd2mdu	1309	2	infection	infection	NN
cord-014597-66vd2mdu	1309	3	took	take	VBD
cord-014597-66vd2mdu	1309	4	place	place	NN
cord-014597-66vd2mdu	1309	5	in	in	IN
cord-014597-66vd2mdu	1309	6	serum	serum	NN
cord-014597-66vd2mdu	1309	7	-	-	HYPH
cord-014597-66vd2mdu	1309	8	free	free	JJ
cord-014597-66vd2mdu	1309	9	media	medium	NNS
cord-014597-66vd2mdu	1309	10	.	.	.
cord-014597-66vd2mdu	1310	1	In	in	IN
cord-014597-66vd2mdu	1310	2	-	-	HYPH
cord-014597-66vd2mdu	1310	3	line	line	NN
cord-014597-66vd2mdu	1310	4	clarification	clarification	NN
cord-014597-66vd2mdu	1310	5	and	and	CC
cord-014597-66vd2mdu	1310	6	purification	purification	NN
cord-014597-66vd2mdu	1310	7	is	be	VBZ
cord-014597-66vd2mdu	1310	8	targeted	target	VBN
cord-014597-66vd2mdu	1310	9	to	to	TO
cord-014597-66vd2mdu	1310	10	be	be	VB
cord-014597-66vd2mdu	1310	11	performed	perform	VBN
cord-014597-66vd2mdu	1310	12	in	in	IN
cord-014597-66vd2mdu	1310	13	only	only	RB
cord-014597-66vd2mdu	1310	14	a	a	DT
cord-014597-66vd2mdu	1310	15	few	few	JJ
cord-014597-66vd2mdu	1310	16	steps	step	NNS
cord-014597-66vd2mdu	1310	17	(	(	-LRB-
cord-014597-66vd2mdu	1310	18	maximum	maximum	VB
cord-014597-66vd2mdu	1310	19	one	one	CD
cord-014597-66vd2mdu	1310	20	of	of	IN
cord-014597-66vd2mdu	1310	21	two	two	CD
cord-014597-66vd2mdu	1310	22	)	)	-RRB-
cord-014597-66vd2mdu	1310	23	without	without	IN
cord-014597-66vd2mdu	1310	24	intermediary	intermediary	JJ
cord-014597-66vd2mdu	1310	25	diafiltration	diafiltration	NN
cord-014597-66vd2mdu	1310	26	.	.	.
cord-014597-66vd2mdu	1311	1	In	in	IN
cord-014597-66vd2mdu	1311	2	such	such	JJ
cord-014597-66vd2mdu	1311	3	configuration	configuration	NN
cord-014597-66vd2mdu	1311	4	,	,	,
cord-014597-66vd2mdu	1311	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1311	6	measured	measure	VBD
cord-014597-66vd2mdu	1311	7	that	that	IN
cord-014597-66vd2mdu	1311	8	Vero	Vero	NNP
cord-014597-66vd2mdu	1311	9	cells	cell	NNS
cord-014597-66vd2mdu	1311	10	can	can	MD
cord-014597-66vd2mdu	1311	11	reach	reach	VB
cord-014597-66vd2mdu	1311	12	a	a	DT
cord-014597-66vd2mdu	1311	13	cell	cell	NN
cord-014597-66vd2mdu	1311	14	density	density	NN
cord-014597-66vd2mdu	1311	15	of	of	IN
cord-014597-66vd2mdu	1311	16	300	300	CD
cord-014597-66vd2mdu	1311	17	-	-	SYM
cord-014597-66vd2mdu	1311	18	350x10	350x10	CD
cord-014597-66vd2mdu	1311	19	3	3	CD
cord-014597-66vd2mdu	1311	20	cells	cell	NNS
cord-014597-66vd2mdu	1311	21	/	/	SYM
cord-014597-66vd2mdu	1311	22	cm	cm	NN
cord-014597-66vd2mdu	1311	23	2	2	CD
cord-014597-66vd2mdu	1311	24	with	with	IN
cord-014597-66vd2mdu	1311	25	PDL	PDL	NNP
cord-014597-66vd2mdu	1311	26	/	/	SYM
cord-014597-66vd2mdu	1311	27	day	day	NN
cord-014597-66vd2mdu	1311	28	of	of	IN
cord-014597-66vd2mdu	1311	29	1.0	1.0	CD
cord-014597-66vd2mdu	1311	30	-	-	SYM
cord-014597-66vd2mdu	1311	31	1.2	1.2	CD
cord-014597-66vd2mdu	1311	32	in	in	IN
cord-014597-66vd2mdu	1311	33	serum	serum	NN
cord-014597-66vd2mdu	1311	34	-	-	HYPH
cord-014597-66vd2mdu	1311	35	containing	contain	VBG
cord-014597-66vd2mdu	1311	36	media	medium	NNS
cord-014597-66vd2mdu	1311	37	.	.	.
cord-014597-66vd2mdu	1312	1	This	this	DT
cord-014597-66vd2mdu	1312	2	new	new	JJ
cord-014597-66vd2mdu	1312	3	facility	facility	NN
cord-014597-66vd2mdu	1312	4	is	be	VBZ
cord-014597-66vd2mdu	1312	5	expected	expect	VBN
cord-014597-66vd2mdu	1312	6	to	to	TO
cord-014597-66vd2mdu	1312	7	manufacture	manufacture	VB
cord-014597-66vd2mdu	1312	8	any	any	DT
cord-014597-66vd2mdu	1312	9	type	type	NN
cord-014597-66vd2mdu	1312	10	of	of	IN
cord-014597-66vd2mdu	1312	11	viral	viral	JJ
cord-014597-66vd2mdu	1312	12	vaccine	vaccine	NN
cord-014597-66vd2mdu	1312	13	at	at	IN
cord-014597-66vd2mdu	1312	14	a	a	DT
cord-014597-66vd2mdu	1312	15	very	very	RB
cord-014597-66vd2mdu	1312	16	low	low	JJ
cord-014597-66vd2mdu	1312	17	cost	cost	NN
cord-014597-66vd2mdu	1312	18	and	and	CC
cord-014597-66vd2mdu	1312	19	could	could	MD
cord-014597-66vd2mdu	1312	20	be	be	VB
cord-014597-66vd2mdu	1312	21	deployed	deploy	VBN
cord-014597-66vd2mdu	1312	22	at	at	IN
cord-014597-66vd2mdu	1312	23	the	the	DT
cord-014597-66vd2mdu	1312	24	site	site	NN
cord-014597-66vd2mdu	1312	25	of	of	IN
cord-014597-66vd2mdu	1312	26	the	the	DT
cord-014597-66vd2mdu	1312	27	manufacturer	manufacturer	NN
cord-014597-66vd2mdu	1312	28	in	in	IN
cord-014597-66vd2mdu	1312	29	emerging	emerge	VBG
cord-014597-66vd2mdu	1312	30	countries	country	NNS
cord-014597-66vd2mdu	1312	31	,	,	,
cord-014597-66vd2mdu	1312	32	killing	kill	VBG
cord-014597-66vd2mdu	1312	33	the	the	DT
cord-014597-66vd2mdu	1312	34	two	two	CD
cord-014597-66vd2mdu	1312	35	birds	bird	NNS
cord-014597-66vd2mdu	1312	36	of	of	IN
cord-014597-66vd2mdu	1312	37	cost	cost	NN
cord-014597-66vd2mdu	1312	38	of	of	IN
cord-014597-66vd2mdu	1312	39	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1312	40	and	and	CC
cord-014597-66vd2mdu	1312	41	distribution	distribution	NN
cord-014597-66vd2mdu	1312	42	with	with	IN
cord-014597-66vd2mdu	1312	43	one	one	CD
cord-014597-66vd2mdu	1312	44	stone	stone	NN
cord-014597-66vd2mdu	1312	45	.	.	.
cord-014597-66vd2mdu	1313	1	The	the	DT
cord-014597-66vd2mdu	1313	2	presentation	presentation	NN
cord-014597-66vd2mdu	1313	3	will	will	MD
cord-014597-66vd2mdu	1313	4	feature	feature	VB
cord-014597-66vd2mdu	1313	5	the	the	DT
cord-014597-66vd2mdu	1313	6	description	description	NN
cord-014597-66vd2mdu	1313	7	of	of	IN
cord-014597-66vd2mdu	1313	8	the	the	DT
cord-014597-66vd2mdu	1313	9	engineering	engineering	NN
cord-014597-66vd2mdu	1313	10	development	development	NN
cord-014597-66vd2mdu	1313	11	,	,	,
cord-014597-66vd2mdu	1313	12	but	but	CC
cord-014597-66vd2mdu	1313	13	also	also	RB
cord-014597-66vd2mdu	1313	14	the	the	DT
cord-014597-66vd2mdu	1313	15	preliminary	preliminary	JJ
cord-014597-66vd2mdu	1313	16	results	result	NNS
cord-014597-66vd2mdu	1313	17	of	of	IN
cord-014597-66vd2mdu	1313	18	cell	cell	NN
cord-014597-66vd2mdu	1313	19	growth	growth	NN
cord-014597-66vd2mdu	1313	20	,	,	,
cord-014597-66vd2mdu	1313	21	infections	infection	NNS
cord-014597-66vd2mdu	1313	22	,	,	,
cord-014597-66vd2mdu	1313	23	and	and	CC
cord-014597-66vd2mdu	1313	24	product	product	NN
cord-014597-66vd2mdu	1313	25	quality	quality	NN
cord-014597-66vd2mdu	1313	26	,	,	,
cord-014597-66vd2mdu	1313	27	as	as	RB
cord-014597-66vd2mdu	1313	28	well	well	RB
cord-014597-66vd2mdu	1313	29	as	as	IN
cord-014597-66vd2mdu	1313	30	a	a	DT
cord-014597-66vd2mdu	1313	31	description	description	NN
cord-014597-66vd2mdu	1313	32	of	of	IN
cord-014597-66vd2mdu	1313	33	the	the	DT
cord-014597-66vd2mdu	1313	34	COGS	cogs	JJ
cord-014597-66vd2mdu	1313	35	calculation	calculation	NN
cord-014597-66vd2mdu	1313	36	.	.	.
cord-014597-66vd2mdu	1314	1	Univercells	Univercells	NNP
cord-014597-66vd2mdu	1314	2	developed	develop	VBD
cord-014597-66vd2mdu	1314	3	a	a	DT
cord-014597-66vd2mdu	1314	4	disruptive	disruptive	JJ
cord-014597-66vd2mdu	1314	5	polio	polio	NN
cord-014597-66vd2mdu	1314	6	vaccine	vaccine	NN
cord-014597-66vd2mdu	1314	7	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1314	8	technology	technology	NN
cord-014597-66vd2mdu	1314	9	exceeding	exceed	VBG
cord-014597-66vd2mdu	1314	10	expectations	expectation	NNS
cord-014597-66vd2mdu	1314	11	when	when	WRB
cord-014597-66vd2mdu	1314	12	compared	compare	VBN
cord-014597-66vd2mdu	1314	13	to	to	IN
cord-014597-66vd2mdu	1314	14	traditional	traditional	JJ
cord-014597-66vd2mdu	1314	15	methodsachieving	methodsachieving	NN
cord-014597-66vd2mdu	1314	16	a	a	DT
cord-014597-66vd2mdu	1314	17	superior	superior	JJ
cord-014597-66vd2mdu	1314	18	result	result	NN
cord-014597-66vd2mdu	1314	19	via	via	IN
cord-014597-66vd2mdu	1314	20	its	-PRON-	PRP$
cord-014597-66vd2mdu	1314	21	all	all	DT
cord-014597-66vd2mdu	1314	22	-	-	HYPH
cord-014597-66vd2mdu	1314	23	in	in	IN
cord-014597-66vd2mdu	1314	24	-	-	HYPH
cord-014597-66vd2mdu	1314	25	one	one	CD
cord-014597-66vd2mdu	1314	26	solution	solution	NN
cord-014597-66vd2mdu	1314	27	of	of	IN
cord-014597-66vd2mdu	1314	28	a	a	DT
cord-014597-66vd2mdu	1314	29	simple	simple	JJ
cord-014597-66vd2mdu	1314	30	,	,	,
cord-014597-66vd2mdu	1314	31	scalable	scalable	JJ
cord-014597-66vd2mdu	1314	32	,	,	,
cord-014597-66vd2mdu	1314	33	and	and	CC
cord-014597-66vd2mdu	1314	34	fully	fully	RB
cord-014597-66vd2mdu	1314	35	-	-	HYPH
cord-014597-66vd2mdu	1314	36	disposable	disposable	JJ
cord-014597-66vd2mdu	1314	37	vaccine	vaccine	NN
cord-014597-66vd2mdu	1314	38	production	production	NN
cord-014597-66vd2mdu	1314	39	platform	platform	NN
cord-014597-66vd2mdu	1314	40	resulting	result	VBG
cord-014597-66vd2mdu	1314	41	in	in	IN
cord-014597-66vd2mdu	1314	42	long	long	JJ
cord-014597-66vd2mdu	1314	43	-	-	HYPH
cord-014597-66vd2mdu	1314	44	term	term	NN
cord-014597-66vd2mdu	1314	45	cost	cost	NN
cord-014597-66vd2mdu	1314	46	-	-	HYPH
cord-014597-66vd2mdu	1314	47	effectiveness	effectiveness	NN
cord-014597-66vd2mdu	1314	48	,	,	,
cord-014597-66vd2mdu	1314	49	flexibility	flexibility	NN
cord-014597-66vd2mdu	1314	50	and	and	CC
cord-014597-66vd2mdu	1314	51	sustainability	sustainability	NN
cord-014597-66vd2mdu	1314	52	:	:	:
cord-014597-66vd2mdu	1314	53	-All	-All	``
cord-014597-66vd2mdu	1314	54	upstream	upstream	RB
cord-014597-66vd2mdu	1314	55	,	,	,
cord-014597-66vd2mdu	1314	56	downstream	downstream	JJ
cord-014597-66vd2mdu	1314	57	and	and	CC
cord-014597-66vd2mdu	1314	58	inactivation	inactivation	NN
cord-014597-66vd2mdu	1314	59	steps	step	NNS
cord-014597-66vd2mdu	1314	60	take	take	VBP
cord-014597-66vd2mdu	1314	61	place	place	NN
cord-014597-66vd2mdu	1314	62	within	within	IN
cord-014597-66vd2mdu	1314	63	a	a	DT
cord-014597-66vd2mdu	1314	64	closed	closed	JJ
cord-014597-66vd2mdu	1314	65	system	system	NN
cord-014597-66vd2mdu	1314	66	with	with	IN
cord-014597-66vd2mdu	1314	67	all	all	PDT
cord-014597-66vd2mdu	1314	68	the	the	DT
cord-014597-66vd2mdu	1314	69	equipment	equipment	NN
cord-014597-66vd2mdu	1314	70	contained	contain	VBN
cord-014597-66vd2mdu	1314	71	in	in	IN
cord-014597-66vd2mdu	1314	72	a	a	DT
cord-014597-66vd2mdu	1314	73	low	low	JJ
cord-014597-66vd2mdu	1314	74	footprint	footprint	NN
cord-014597-66vd2mdu	1314	75	isolatorcreating	isolatorcreate	VBG
cord-014597-66vd2mdu	1314	76	a	a	DT
cord-014597-66vd2mdu	1314	77	confined	confine	VBN
cord-014597-66vd2mdu	1314	78	area	area	NN
cord-014597-66vd2mdu	1314	79	for	for	IN
cord-014597-66vd2mdu	1314	80	polio	polio	NN
cord-014597-66vd2mdu	1314	81	virus	virus	NN
cord-014597-66vd2mdu	1314	82	handling	handle	VBG
cord-014597-66vd2mdu	1314	83	that	that	WDT
cord-014597-66vd2mdu	1314	84	facilitates	facilitate	VBZ
cord-014597-66vd2mdu	1314	85	the	the	DT
cord-014597-66vd2mdu	1314	86	deployment	deployment	NN
cord-014597-66vd2mdu	1314	87	of	of	IN
cord-014597-66vd2mdu	1314	88	micro	micro	NNS
cord-014597-66vd2mdu	1314	89	-	-	NNS
cord-014597-66vd2mdu	1314	90	facilities	facility	NNS
cord-014597-66vd2mdu	1314	91	.	.	.
cord-014597-66vd2mdu	1315	1	-This	-this	NN
cord-014597-66vd2mdu	1315	2	leads	lead	VBZ
cord-014597-66vd2mdu	1315	3	to	to	IN
cord-014597-66vd2mdu	1315	4	a	a	DT
cord-014597-66vd2mdu	1315	5	dramatic	dramatic	JJ
cord-014597-66vd2mdu	1315	6	reduction	reduction	NN
cord-014597-66vd2mdu	1315	7	in	in	IN
cord-014597-66vd2mdu	1315	8	capital	capital	NN
cord-014597-66vd2mdu	1315	9	investment	investment	NN
cord-014597-66vd2mdu	1315	10	,	,	,
cord-014597-66vd2mdu	1315	11	time	time	NN
cord-014597-66vd2mdu	1315	12	required	require	VBN
cord-014597-66vd2mdu	1315	13	for	for	IN
cord-014597-66vd2mdu	1315	14	development	development	NN
cord-014597-66vd2mdu	1315	15	and	and	CC
cord-014597-66vd2mdu	1315	16	increases	increase	NNS
cord-014597-66vd2mdu	1315	17	production	production	NN
cord-014597-66vd2mdu	1315	18	capacity	capacity	NN
cord-014597-66vd2mdu	1315	19	.	.	.
cord-014597-66vd2mdu	1316	1	-In	-In	:
cord-014597-66vd2mdu	1316	2	conclusion	conclusion	NN
cord-014597-66vd2mdu	1316	3	,	,	,
cord-014597-66vd2mdu	1316	4	this	this	DT
cord-014597-66vd2mdu	1316	5	is	be	VBZ
cord-014597-66vd2mdu	1316	6	a	a	DT
cord-014597-66vd2mdu	1316	7	simple	simple	JJ
cord-014597-66vd2mdu	1316	8	and	and	CC
cord-014597-66vd2mdu	1316	9	elegant	elegant	JJ
cord-014597-66vd2mdu	1316	10	solution	solution	NN
cord-014597-66vd2mdu	1316	11	for	for	IN
cord-014597-66vd2mdu	1316	12	the	the	DT
cord-014597-66vd2mdu	1316	13	industrial	industrial	JJ
cord-014597-66vd2mdu	1316	14	production	production	NN
cord-014597-66vd2mdu	1316	15	of	of	IN
cord-014597-66vd2mdu	1316	16	human	human	JJ
cord-014597-66vd2mdu	1316	17	vaccines	vaccine	NNS
cord-014597-66vd2mdu	1316	18	at	at	IN
cord-014597-66vd2mdu	1316	19	a	a	DT
cord-014597-66vd2mdu	1316	20	low	low	JJ
cord-014597-66vd2mdu	1316	21	cost	cost	NN
cord-014597-66vd2mdu	1316	22	in	in	IN
cord-014597-66vd2mdu	1316	23	micro	micro	JJ
cord-014597-66vd2mdu	1316	24	-	-	NNS
cord-014597-66vd2mdu	1316	25	facilities	facility	NNS
cord-014597-66vd2mdu	1316	26	,	,	,
cord-014597-66vd2mdu	1316	27	making	make	VBG
cord-014597-66vd2mdu	1316	28	polio	polio	NN
cord-014597-66vd2mdu	1316	29	vaccines	vaccine	NNS
cord-014597-66vd2mdu	1316	30	available	available	JJ
cord-014597-66vd2mdu	1316	31	to	to	IN
cord-014597-66vd2mdu	1316	32	all	all	DT
cord-014597-66vd2mdu	1316	33	.	.	.
cord-014597-66vd2mdu	1317	1	Comparison	comparison	NN
cord-014597-66vd2mdu	1317	2	of	of	IN
cord-014597-66vd2mdu	1317	3	media	medium	NNS
cord-014597-66vd2mdu	1317	4	formulations	formulation	NNS
cord-014597-66vd2mdu	1317	5	for	for	IN
cord-014597-66vd2mdu	1317	6	the	the	DT
cord-014597-66vd2mdu	1317	7	vaccine	vaccine	NN
cord-014597-66vd2mdu	1317	8	production	production	NN
cord-014597-66vd2mdu	1317	9	in	in	IN
cord-014597-66vd2mdu	1317	10	1	1	CD
cord-014597-66vd2mdu	1317	11	L	l	NN
cord-014597-66vd2mdu	1317	12	stirred	stir	VBN
cord-014597-66vd2mdu	1317	13	tank	tank	NN
cord-014597-66vd2mdu	1317	14	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	1317	15	operated	operate	VBN
cord-014597-66vd2mdu	1317	16	in	in	IN
cord-014597-66vd2mdu	1317	17	continuous	continuous	JJ
cord-014597-66vd2mdu	1317	18	mode	mode	NN
cord-014597-66vd2mdu	1317	19	.	.	.
cord-014597-66vd2mdu	1318	1	Both	both	DT
cord-014597-66vd2mdu	1318	2	cultures	culture	NNS
cord-014597-66vd2mdu	1318	3	were	be	VBD
cord-014597-66vd2mdu	1318	4	performed	perform	VBN
cord-014597-66vd2mdu	1318	5	in	in	IN
cord-014597-66vd2mdu	1318	6	parallel	parallel	NN
cord-014597-66vd2mdu	1318	7	using	use	VBG
cord-014597-66vd2mdu	1318	8	the	the	DT
cord-014597-66vd2mdu	1318	9	corresponding	corresponding	JJ
cord-014597-66vd2mdu	1318	10	medium	medium	NN
cord-014597-66vd2mdu	1318	11	for	for	IN
cord-014597-66vd2mdu	1318	12	the	the	DT
cord-014597-66vd2mdu	1318	13	perfusion	perfusion	NN
cord-014597-66vd2mdu	1318	14	.	.	.
cord-014597-66vd2mdu	1319	1	a	a	DT
cord-014597-66vd2mdu	1319	2	Feeding	feeding	NN
cord-014597-66vd2mdu	1319	3	was	be	VBD
cord-014597-66vd2mdu	1319	4	performed	perform	VBN
cord-014597-66vd2mdu	1319	5	with	with	IN
cord-014597-66vd2mdu	1319	6	the	the	DT
cord-014597-66vd2mdu	1319	7	commercial	commercial	JJ
cord-014597-66vd2mdu	1319	8	SFM	SFM	NNP
cord-014597-66vd2mdu	1319	9	.	.	.
cord-014597-66vd2mdu	1320	1	b	b	LS
cord-014597-66vd2mdu	1320	2	The	the	DT
cord-014597-66vd2mdu	1320	3	first	first	JJ
cord-014597-66vd2mdu	1320	4	two	two	CD
cord-014597-66vd2mdu	1320	5	days	day	NNS
cord-014597-66vd2mdu	1320	6	of	of	IN
cord-014597-66vd2mdu	1320	7	perfusion	perfusion	NN
cord-014597-66vd2mdu	1320	8	feeding	feeding	NN
cord-014597-66vd2mdu	1320	9	was	be	VBD
cord-014597-66vd2mdu	1320	10	performed	perform	VBN
cord-014597-66vd2mdu	1320	11	with	with	IN
cord-014597-66vd2mdu	1320	12	SFM	SFM	NNP
cord-014597-66vd2mdu	1320	13	until	until	IN
cord-014597-66vd2mdu	1320	14	the	the	DT
cord-014597-66vd2mdu	1320	15	cell	cell	NN
cord-014597-66vd2mdu	1320	16	density	density	NN
cord-014597-66vd2mdu	1320	17	reach	reach	VBP
cord-014597-66vd2mdu	1320	18	10	10	CD
cord-014597-66vd2mdu	1320	19	7	7	CD
cord-014597-66vd2mdu	1320	20	cells.ml	cells.ml	CD
cord-014597-66vd2mdu	1320	21	-1	-1	CD
cord-014597-66vd2mdu	1321	1	and	and	CC
cord-014597-66vd2mdu	1321	2	,	,	,
cord-014597-66vd2mdu	1321	3	after	after	IN
cord-014597-66vd2mdu	1321	4	that	that	DT
cord-014597-66vd2mdu	1321	5	,	,	,
cord-014597-66vd2mdu	1321	6	the	the	DT
cord-014597-66vd2mdu	1321	7	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1321	8	was	be	VBD
cord-014597-66vd2mdu	1321	9	fed	feed	VBN
cord-014597-66vd2mdu	1321	10	using	use	VBG
cord-014597-66vd2mdu	1321	11	the	the	DT
cord-014597-66vd2mdu	1321	12	RVPM	RVPM	NNP
cord-014597-66vd2mdu	1321	13	formulation	formulation	NN
cord-014597-66vd2mdu	1321	14	.	.	.
cord-014597-66vd2mdu	1322	1	(	(	-LRB-
cord-014597-66vd2mdu	1322	2	↓	↓	NFP
cord-014597-66vd2mdu	1322	3	)	)	-RRB-
cord-014597-66vd2mdu	1322	4	On	on	IN
cord-014597-66vd2mdu	1322	5	day	day	NN
cord-014597-66vd2mdu	1322	6	number	number	NN
cord-014597-66vd2mdu	1322	7	10	10	CD
cord-014597-66vd2mdu	1322	8	,	,	,
cord-014597-66vd2mdu	1322	9	20	20	CD
cord-014597-66vd2mdu	1322	10	%	%	NN
cord-014597-66vd2mdu	1322	11	of	of	IN
cord-014597-66vd2mdu	1322	12	the	the	DT
cord-014597-66vd2mdu	1322	13	reactor	reactor	NN
cord-014597-66vd2mdu	1322	14	volume	volume	NN
cord-014597-66vd2mdu	1322	15	was	be	VBD
cord-014597-66vd2mdu	1322	16	punctually	punctually	RB
cord-014597-66vd2mdu	1322	17	bled	bleed	VBN
cord-014597-66vd2mdu	1322	18	maintaining	maintain	VBG
cord-014597-66vd2mdu	1322	19	the	the	DT
cord-014597-66vd2mdu	1322	20	working	work	VBG
cord-014597-66vd2mdu	1322	21	volume	volume	NN
cord-014597-66vd2mdu	1322	22	Background	Background	NNP
cord-014597-66vd2mdu	1322	23	Vectored	vectore	VBD
cord-014597-66vd2mdu	1322	24	vaccines	vaccine	NNS
cord-014597-66vd2mdu	1322	25	based	base	VBN
cord-014597-66vd2mdu	1322	26	on	on	IN
cord-014597-66vd2mdu	1322	27	modified	modify	VBN
cord-014597-66vd2mdu	1322	28	vaccinia	vaccinia	NN
cord-014597-66vd2mdu	1322	29	virus	virus	NN
cord-014597-66vd2mdu	1323	1	Ankara	Ankara	NNP
cord-014597-66vd2mdu	1323	2	(	(	-LRB-
cord-014597-66vd2mdu	1323	3	MVA	MVA	NNP
cord-014597-66vd2mdu	1323	4	)	)	-RRB-
cord-014597-66vd2mdu	1323	5	are	be	VBP
cord-014597-66vd2mdu	1323	6	reported	report	VBN
cord-014597-66vd2mdu	1323	7	to	to	TO
cord-014597-66vd2mdu	1323	8	stably	stably	RB
cord-014597-66vd2mdu	1323	9	maintain	maintain	VB
cord-014597-66vd2mdu	1323	10	large	large	JJ
cord-014597-66vd2mdu	1323	11	transgenes	transgene	NNS
cord-014597-66vd2mdu	1323	12	,	,	,
cord-014597-66vd2mdu	1323	13	and	and	CC
cord-014597-66vd2mdu	1323	14	to	to	TO
cord-014597-66vd2mdu	1323	15	be	be	VB
cord-014597-66vd2mdu	1323	16	safe	safe	JJ
cord-014597-66vd2mdu	1323	17	,	,	,
cord-014597-66vd2mdu	1323	18	immunogenic	immunogenic	JJ
cord-014597-66vd2mdu	1323	19	and	and	CC
cord-014597-66vd2mdu	1323	20	tolerant	tolerant	JJ
cord-014597-66vd2mdu	1323	21	to	to	TO
cord-014597-66vd2mdu	1323	22	pre	pre	VB
cord-014597-66vd2mdu	1323	23	-	-	JJ
cord-014597-66vd2mdu	1323	24	existing	existing	JJ
cord-014597-66vd2mdu	1323	25	immunity	immunity	NN
cord-014597-66vd2mdu	1323	26	.	.	.
cord-014597-66vd2mdu	1324	1	MVA	MVA	NNP
cord-014597-66vd2mdu	1324	2	is	be	VBZ
cord-014597-66vd2mdu	1324	3	usually	usually	RB
cord-014597-66vd2mdu	1324	4	produced	produce	VBN
cord-014597-66vd2mdu	1324	5	on	on	IN
cord-014597-66vd2mdu	1324	6	primary	primary	JJ
cord-014597-66vd2mdu	1324	7	chicken	chicken	NN
cord-014597-66vd2mdu	1324	8	embryo	embryo	NN
cord-014597-66vd2mdu	1324	9	fibroblasts	fibroblast	NNS
cord-014597-66vd2mdu	1324	10	but	but	CC
cord-014597-66vd2mdu	1324	11	continuous	continuous	JJ
cord-014597-66vd2mdu	1324	12	cell	cell	NN
cord-014597-66vd2mdu	1324	13	lines	line	NNS
cord-014597-66vd2mdu	1324	14	are	be	VBP
cord-014597-66vd2mdu	1324	15	being	be	VBG
cord-014597-66vd2mdu	1324	16	investigated	investigate	VBN
cord-014597-66vd2mdu	1324	17	as	as	IN
cord-014597-66vd2mdu	1324	18	more	more	RBR
cord-014597-66vd2mdu	1324	19	versatile	versatile	JJ
cord-014597-66vd2mdu	1324	20	substrates	substrate	NNS
cord-014597-66vd2mdu	1324	21	.	.	.
cord-014597-66vd2mdu	1325	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1325	2	have	have	VBP
cord-014597-66vd2mdu	1325	3	previously	previously	RB
cord-014597-66vd2mdu	1325	4	reported	report	VBN
cord-014597-66vd2mdu	1325	5	development	development	NN
cord-014597-66vd2mdu	1325	6	of	of	IN
cord-014597-66vd2mdu	1325	7	a	a	DT
cord-014597-66vd2mdu	1325	8	continuous	continuous	JJ
cord-014597-66vd2mdu	1325	9	suspension	suspension	NN
cord-014597-66vd2mdu	1325	10	cell	cell	NN
cord-014597-66vd2mdu	1325	11	line	line	NN
cord-014597-66vd2mdu	1325	12	(	(	-LRB-
cord-014597-66vd2mdu	1325	13	CR.pIX	CR.pIX	NNP
cord-014597-66vd2mdu	1325	14	)	)	-RRB-
cord-014597-66vd2mdu	1325	15	derived	derive	VBN
cord-014597-66vd2mdu	1325	16	from	from	IN
cord-014597-66vd2mdu	1325	17	the	the	DT
cord-014597-66vd2mdu	1325	18	muscovy	muscovy	NNP
cord-014597-66vd2mdu	1325	19	duck	duck	NN
cord-014597-66vd2mdu	1325	20	and	and	CC
cord-014597-66vd2mdu	1325	21	efficient	efficient	JJ
cord-014597-66vd2mdu	1325	22	production	production	NN
cord-014597-66vd2mdu	1325	23	process	process	NN
cord-014597-66vd2mdu	1325	24	for	for	IN
cord-014597-66vd2mdu	1325	25	MVA	MVA	NNP
cord-014597-66vd2mdu	1325	26	in	in	IN
cord-014597-66vd2mdu	1325	27	chemically	chemically	RB
cord-014597-66vd2mdu	1325	28	defined	define	VBN
cord-014597-66vd2mdu	1325	29	media	medium	NNS
cord-014597-66vd2mdu	1325	30	[	[	-LRB-
cord-014597-66vd2mdu	1325	31	1	1	CD
cord-014597-66vd2mdu	1325	32	,	,	,
cord-014597-66vd2mdu	1325	33	2	2	CD
cord-014597-66vd2mdu	1325	34	]	]	-RRB-
cord-014597-66vd2mdu	1325	35	.	.	.
cord-014597-66vd2mdu	1326	1	This	this	DT
cord-014597-66vd2mdu	1326	2	process	process	NN
cord-014597-66vd2mdu	1326	3	allowed	allow	VBD
cord-014597-66vd2mdu	1326	4	isolation	isolation	NN
cord-014597-66vd2mdu	1326	5	of	of	IN
cord-014597-66vd2mdu	1326	6	an	an	DT
cord-014597-66vd2mdu	1326	7	hitherto	hitherto	RB
cord-014597-66vd2mdu	1326	8	undescribed	undescribed	JJ
cord-014597-66vd2mdu	1326	9	genotype	genotype	NN
cord-014597-66vd2mdu	1326	10	(	(	-LRB-
cord-014597-66vd2mdu	1326	11	MVA	MVA	NNP
cord-014597-66vd2mdu	1326	12	-	-	HYPH
cord-014597-66vd2mdu	1326	13	CR19	CR19	NNP
cord-014597-66vd2mdu	1326	14	)	)	-RRB-
cord-014597-66vd2mdu	1326	15	that	that	WDT
cord-014597-66vd2mdu	1326	16	induced	induce	VBD
cord-014597-66vd2mdu	1326	17	fewer	few	JJR
cord-014597-66vd2mdu	1326	18	syncytia	syncytia	NN
cord-014597-66vd2mdu	1326	19	in	in	IN
cord-014597-66vd2mdu	1326	20	adherent	adherent	JJ
cord-014597-66vd2mdu	1326	21	cultures	culture	NNS
cord-014597-66vd2mdu	1326	22	and	and	CC
cord-014597-66vd2mdu	1326	23	replicated	replicate	VBD
cord-014597-66vd2mdu	1326	24	to	to	IN
cord-014597-66vd2mdu	1326	25	higher	high	JJR
cord-014597-66vd2mdu	1326	26	infectious	infectious	JJ
cord-014597-66vd2mdu	1326	27	titers	titer	NNS
cord-014597-66vd2mdu	1326	28	in	in	IN
cord-014597-66vd2mdu	1326	29	the	the	DT
cord-014597-66vd2mdu	1326	30	extracellular	extracellular	JJ
cord-014597-66vd2mdu	1326	31	volume	volume	NN
cord-014597-66vd2mdu	1326	32	of	of	IN
cord-014597-66vd2mdu	1326	33	suspension	suspension	NN
cord-014597-66vd2mdu	1326	34	cultures	culture	NNS
cord-014597-66vd2mdu	1326	35	[	[	-LRB-
cord-014597-66vd2mdu	1326	36	3	3	CD
cord-014597-66vd2mdu	1326	37	]	]	-RRB-
cord-014597-66vd2mdu	1326	38	.	.	.
cord-014597-66vd2mdu	1327	1	Replication	replication	NN
cord-014597-66vd2mdu	1327	2	of	of	IN
cord-014597-66vd2mdu	1327	3	MVA	MVA	NNP
cord-014597-66vd2mdu	1327	4	-	-	HYPH
cord-014597-66vd2mdu	1327	5	CR19	CR19	NNP
cord-014597-66vd2mdu	1327	6	remained	remain	VBD
cord-014597-66vd2mdu	1327	7	restricted	restrict	VBN
cord-014597-66vd2mdu	1327	8	predominantely	predominantely	RB
cord-014597-66vd2mdu	1327	9	to	to	IN
cord-014597-66vd2mdu	1327	10	avian	avian	JJ
cord-014597-66vd2mdu	1327	11	cells	cell	NNS
cord-014597-66vd2mdu	1327	12	,	,	,
cord-014597-66vd2mdu	1327	13	an	an	DT
cord-014597-66vd2mdu	1327	14	important	important	JJ
cord-014597-66vd2mdu	1327	15	property	property	NN
cord-014597-66vd2mdu	1327	16	of	of	IN
cord-014597-66vd2mdu	1327	17	MVA	MVA	NNP
cord-014597-66vd2mdu	1327	18	vectors	vector	NNS
cord-014597-66vd2mdu	1327	19	.	.	.
cord-014597-66vd2mdu	1328	1	Homologous	homologous	JJ
cord-014597-66vd2mdu	1328	2	recombination	recombination	NN
cord-014597-66vd2mdu	1328	3	in	in	IN
cord-014597-66vd2mdu	1328	4	CR.pIX	CR.pIX	NNP
cord-014597-66vd2mdu	1328	5	cells	cell	NNS
cord-014597-66vd2mdu	1328	6	was	be	VBD
cord-014597-66vd2mdu	1328	7	used	use	VBN
cord-014597-66vd2mdu	1328	8	to	to	TO
cord-014597-66vd2mdu	1328	9	generate	generate	VB
cord-014597-66vd2mdu	1328	10	viruses	virus	NNS
cord-014597-66vd2mdu	1328	11	with	with	IN
cord-014597-66vd2mdu	1328	12	various	various	JJ
cord-014597-66vd2mdu	1328	13	expression	expression	NN
cord-014597-66vd2mdu	1328	14	cassettes	cassette	NNS
cord-014597-66vd2mdu	1328	15	in	in	IN
cord-014597-66vd2mdu	1328	16	deletion	deletion	NN
cord-014597-66vd2mdu	1328	17	site	site	NN
cord-014597-66vd2mdu	1328	18	III	iii	CD
cord-014597-66vd2mdu	1328	19	[	[	-LRB-
cord-014597-66vd2mdu	1328	20	4	4	CD
cord-014597-66vd2mdu	1328	21	]	]	-RRB-
cord-014597-66vd2mdu	1328	22	and	and	CC
cord-014597-66vd2mdu	1328	23	combinations	combination	NNS
cord-014597-66vd2mdu	1328	24	of	of	IN
cord-014597-66vd2mdu	1328	25	the	the	DT
cord-014597-66vd2mdu	1328	26	differentiating	differentiating	JJ
cord-014597-66vd2mdu	1328	27	point	point	NN
cord-014597-66vd2mdu	1328	28	mutations	mutation	NNS
cord-014597-66vd2mdu	1328	29	of	of	IN
cord-014597-66vd2mdu	1328	30	MVA	MVA	NNP
cord-014597-66vd2mdu	1328	31	-	-	HYPH
cord-014597-66vd2mdu	1328	32	CR19	CR19	NNP
cord-014597-66vd2mdu	1328	33	in	in	IN
cord-014597-66vd2mdu	1328	34	a	a	DT
cord-014597-66vd2mdu	1328	35	backbone	backbone	NN
cord-014597-66vd2mdu	1328	36	of	of	IN
cord-014597-66vd2mdu	1328	37	wildtype	wildtype	JJ
cord-014597-66vd2mdu	1328	38	virus	virus	NN
cord-014597-66vd2mdu	1328	39	.	.	.
cord-014597-66vd2mdu	1329	1	All	all	DT
cord-014597-66vd2mdu	1329	2	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1329	3	viruses	virus	NNS
cord-014597-66vd2mdu	1329	4	were	be	VBD
cord-014597-66vd2mdu	1329	5	plaquepurified	plaquepurifie	VBN
cord-014597-66vd2mdu	1329	6	.	.	.
cord-014597-66vd2mdu	1330	1	Successful	successful	JJ
cord-014597-66vd2mdu	1330	2	introduction	introduction	NN
cord-014597-66vd2mdu	1330	3	of	of	IN
cord-014597-66vd2mdu	1330	4	the	the	DT
cord-014597-66vd2mdu	1330	5	mutations	mutation	NNS
cord-014597-66vd2mdu	1330	6	was	be	VBD
cord-014597-66vd2mdu	1330	7	confirmed	confirm	VBN
cord-014597-66vd2mdu	1330	8	by	by	IN
cord-014597-66vd2mdu	1330	9	sequencing	sequence	VBG
cord-014597-66vd2mdu	1330	10	and	and	CC
cord-014597-66vd2mdu	1330	11	specifically	specifically	RB
cord-014597-66vd2mdu	1330	12	designed	design	VBN
cord-014597-66vd2mdu	1330	13	restriction	restriction	NN
cord-014597-66vd2mdu	1330	14	fragment	fragment	NN
cord-014597-66vd2mdu	1330	15	length	length	NN
cord-014597-66vd2mdu	1330	16	polymorphisms	polymorphism	NNS
cord-014597-66vd2mdu	1330	17	(	(	-LRB-
cord-014597-66vd2mdu	1330	18	RFLPs	rflp	NNS
cord-014597-66vd2mdu	1330	19	)	)	-RRB-
cord-014597-66vd2mdu	1330	20	.	.	.
cord-014597-66vd2mdu	1331	1	Viruses	virus	NNS
cord-014597-66vd2mdu	1331	2	were	be	VBD
cord-014597-66vd2mdu	1331	3	analyzed	analyze	VBN
cord-014597-66vd2mdu	1331	4	by	by	IN
cord-014597-66vd2mdu	1331	5	serial	serial	JJ
cord-014597-66vd2mdu	1331	6	passaging	passaging	NN
cord-014597-66vd2mdu	1331	7	,	,	,
cord-014597-66vd2mdu	1331	8	diagnostic	diagnostic	JJ
cord-014597-66vd2mdu	1331	9	PCRs	pcr	NNS
cord-014597-66vd2mdu	1331	10	accross	accross	IN
cord-014597-66vd2mdu	1331	11	deletion	deletion	NN
cord-014597-66vd2mdu	1331	12	sites	site	NNS
cord-014597-66vd2mdu	1331	13	[	[	-LRB-
cord-014597-66vd2mdu	1331	14	4	4	CD
cord-014597-66vd2mdu	1331	15	]	]	-RRB-
cord-014597-66vd2mdu	1331	16	,	,	,
cord-014597-66vd2mdu	1331	17	replication	replication	NN
cord-014597-66vd2mdu	1331	18	kinetics	kinetics	NN
cord-014597-66vd2mdu	1331	19	,	,	,
cord-014597-66vd2mdu	1331	20	plaque	plaque	NN
cord-014597-66vd2mdu	1331	21	phenotype	phenotype	NN
cord-014597-66vd2mdu	1331	22	and	and	CC
cord-014597-66vd2mdu	1331	23	electron	electron	NN
cord-014597-66vd2mdu	1331	24	microscopy	microscopy	NN
cord-014597-66vd2mdu	1331	25	.	.	.
cord-014597-66vd2mdu	1332	1	The	the	DT
cord-014597-66vd2mdu	1332	2	genome	genome	NN
cord-014597-66vd2mdu	1332	3	was	be	VBD
cord-014597-66vd2mdu	1332	4	further	further	RB
cord-014597-66vd2mdu	1332	5	investigated	investigate	VBN
cord-014597-66vd2mdu	1332	6	by	by	IN
cord-014597-66vd2mdu	1332	7	anchored	anchor	VBN
cord-014597-66vd2mdu	1332	8	PCR	PCR	NNP
cord-014597-66vd2mdu	1332	9	and	and	CC
cord-014597-66vd2mdu	1332	10	long	long	JJ
cord-014597-66vd2mdu	1332	11	PCR	PCR	NNP
cord-014597-66vd2mdu	1332	12	.	.	.
cord-014597-66vd2mdu	1333	1	Efficiency	efficiency	NN
cord-014597-66vd2mdu	1333	2	of	of	IN
cord-014597-66vd2mdu	1333	3	spread	spread	NN
cord-014597-66vd2mdu	1333	4	of	of	IN
cord-014597-66vd2mdu	1333	5	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1333	6	viruses	virus	NNS
cord-014597-66vd2mdu	1333	7	(	(	-LRB-
cord-014597-66vd2mdu	1333	8	Fig	Fig	NNP
cord-014597-66vd2mdu	1333	9	.	.	.
cord-014597-66vd2mdu	1333	10	1a	1a	CD
cord-014597-66vd2mdu	1333	11	)	)	-RRB-
cord-014597-66vd2mdu	1333	12	could	could	MD
cord-014597-66vd2mdu	1333	13	be	be	VB
cord-014597-66vd2mdu	1333	14	mapped	map	VBN
cord-014597-66vd2mdu	1333	15	to	to	IN
cord-014597-66vd2mdu	1333	16	a	a	DT
cord-014597-66vd2mdu	1333	17	point	point	NN
cord-014597-66vd2mdu	1333	18	mutation	mutation	NN
cord-014597-66vd2mdu	1333	19	in	in	IN
cord-014597-66vd2mdu	1333	20	one	one	CD
cord-014597-66vd2mdu	1333	21	of	of	IN
cord-014597-66vd2mdu	1333	22	the	the	DT
cord-014597-66vd2mdu	1333	23	genes	gene	NNS
cord-014597-66vd2mdu	1333	24	,	,	,
cord-014597-66vd2mdu	1334	1	A34R.	a34r.	UH
cord-014597-66vd2mdu	1335	1	However	however	RB
cord-014597-66vd2mdu	1335	2	,	,	,
cord-014597-66vd2mdu	1335	3	although	although	IN
cord-014597-66vd2mdu	1335	4	MVA	MVA	NNP
cord-014597-66vd2mdu	1335	5	-	-	HYPH
cord-014597-66vd2mdu	1335	6	CR19	CR19	NNP
cord-014597-66vd2mdu	1335	7	carries	carry	VBZ
cord-014597-66vd2mdu	1335	8	mutations	mutation	NNS
cord-014597-66vd2mdu	1335	9	in	in	IN
cord-014597-66vd2mdu	1335	10	three	three	CD
cord-014597-66vd2mdu	1335	11	structural	structural	JJ
cord-014597-66vd2mdu	1335	12	proteins	protein	NNS
cord-014597-66vd2mdu	1335	13	we	-PRON-	PRP
cord-014597-66vd2mdu	1335	14	detected	detect	VBD
cord-014597-66vd2mdu	1335	15	no	no	DT
cord-014597-66vd2mdu	1335	16	obvious	obvious	JJ
cord-014597-66vd2mdu	1335	17	differences	difference	NNS
cord-014597-66vd2mdu	1335	18	to	to	IN
cord-014597-66vd2mdu	1335	19	wildtype	wildtype	NN
cord-014597-66vd2mdu	1335	20	by	by	IN
cord-014597-66vd2mdu	1335	21	electron	electron	NN
cord-014597-66vd2mdu	1335	22	microscopy	microscopy	NNS
cord-014597-66vd2mdu	1335	23	(	(	-LRB-
cord-014597-66vd2mdu	1335	24	Fig	fig	NN
cord-014597-66vd2mdu	1335	25	.	.	.
cord-014597-66vd2mdu	1335	26	1b	1b	CD
cord-014597-66vd2mdu	1335	27	)	)	-RRB-
cord-014597-66vd2mdu	1335	28	.	.	.
cord-014597-66vd2mdu	1336	1	The	the	DT
cord-014597-66vd2mdu	1336	2	replacement	replacement	NN
cord-014597-66vd2mdu	1336	3	of	of	IN
cord-014597-66vd2mdu	1336	4	the	the	DT
cord-014597-66vd2mdu	1336	5	left	left	JJ
cord-014597-66vd2mdu	1336	6	viral	viral	JJ
cord-014597-66vd2mdu	1336	7	telomere	telomere	NN
cord-014597-66vd2mdu	1336	8	by	by	IN
cord-014597-66vd2mdu	1336	9	the	the	DT
cord-014597-66vd2mdu	1336	10	right	right	JJ
cord-014597-66vd2mdu	1336	11	counterpart	counterpart	NN
cord-014597-66vd2mdu	1336	12	was	be	VBD
cord-014597-66vd2mdu	1336	13	the	the	DT
cord-014597-66vd2mdu	1336	14	most	most	RBS
cord-014597-66vd2mdu	1336	15	surprising	surprising	JJ
cord-014597-66vd2mdu	1336	16	result	result	NN
cord-014597-66vd2mdu	1336	17	of	of	IN
cord-014597-66vd2mdu	1336	18	our	-PRON-	PRP$
cord-014597-66vd2mdu	1336	19	new	new	JJ
cord-014597-66vd2mdu	1336	20	study	study	NN
cord-014597-66vd2mdu	1336	21	(	(	-LRB-
cord-014597-66vd2mdu	1336	22	Fig	Fig	NNP
cord-014597-66vd2mdu	1336	23	.	.	.
cord-014597-66vd2mdu	1336	24	1c	1c	CD
cord-014597-66vd2mdu	1336	25	)	)	-RRB-
cord-014597-66vd2mdu	1336	26	.	.	.
cord-014597-66vd2mdu	1337	1	This	this	DT
cord-014597-66vd2mdu	1337	2	extensive	extensive	JJ
cord-014597-66vd2mdu	1337	3	rearrangement	rearrangement	NN
cord-014597-66vd2mdu	1337	4	affects	affect	VBZ
cord-014597-66vd2mdu	1337	5	15	15	CD
cord-014597-66vd2mdu	1337	6	%	%	NN
cord-014597-66vd2mdu	1337	7	of	of	IN
cord-014597-66vd2mdu	1337	8	the	the	DT
cord-014597-66vd2mdu	1337	9	viral	viral	JJ
cord-014597-66vd2mdu	1337	10	genome	genome	NN
cord-014597-66vd2mdu	1337	11	and	and	CC
cord-014597-66vd2mdu	1337	12	has	have	VBZ
cord-014597-66vd2mdu	1337	13	also	also	RB
cord-014597-66vd2mdu	1337	14	increased	increase	VBN
cord-014597-66vd2mdu	1337	15	the	the	DT
cord-014597-66vd2mdu	1337	16	area	area	NN
cord-014597-66vd2mdu	1337	17	of	of	IN
cord-014597-66vd2mdu	1337	18	complementarity	complementarity	NN
cord-014597-66vd2mdu	1337	19	between	between	IN
cord-014597-66vd2mdu	1337	20	the	the	DT
cord-014597-66vd2mdu	1337	21	two	two	CD
cord-014597-66vd2mdu	1337	22	telomeres	telomere	NNS
cord-014597-66vd2mdu	1337	23	.	.	.
cord-014597-66vd2mdu	1338	1	The	the	DT
cord-014597-66vd2mdu	1338	2	recombination	recombination	NN
cord-014597-66vd2mdu	1338	3	site	site	NN
cord-014597-66vd2mdu	1338	4	was	be	VBD
cord-014597-66vd2mdu	1338	5	precisely	precisely	RB
cord-014597-66vd2mdu	1338	6	located	locate	VBN
cord-014597-66vd2mdu	1338	7	and	and	CC
cord-014597-66vd2mdu	1338	8	shown	show	VBN
cord-014597-66vd2mdu	1338	9	via	via	IN
cord-014597-66vd2mdu	1338	10	analysis	analysis	NN
cord-014597-66vd2mdu	1338	11	of	of	IN
cord-014597-66vd2mdu	1338	12	earlier	early	JJR
cord-014597-66vd2mdu	1338	13	and	and	CC
cord-014597-66vd2mdu	1338	14	subsequent	subsequent	JJ
cord-014597-66vd2mdu	1338	15	passages	passage	NNS
cord-014597-66vd2mdu	1338	16	to	to	TO
cord-014597-66vd2mdu	1338	17	be	be	VB
cord-014597-66vd2mdu	1338	18	a	a	DT
cord-014597-66vd2mdu	1338	19	stable	stable	JJ
cord-014597-66vd2mdu	1338	20	property	property	NN
cord-014597-66vd2mdu	1338	21	of	of	IN
cord-014597-66vd2mdu	1338	22	MVA	MVA	NNP
cord-014597-66vd2mdu	1338	23	-	-	HYPH
cord-014597-66vd2mdu	1338	24	CR19	CR19	NNP
cord-014597-66vd2mdu	1338	25	.	.	.
cord-014597-66vd2mdu	1339	1	Various	various	JJ
cord-014597-66vd2mdu	1339	2	viruses	virus	NNS
cord-014597-66vd2mdu	1339	3	,	,	,
cord-014597-66vd2mdu	1339	4	including	include	VBG
cord-014597-66vd2mdu	1339	5	those	those	DT
cord-014597-66vd2mdu	1339	6	with	with	IN
cord-014597-66vd2mdu	1339	7	larger	large	JJR
cord-014597-66vd2mdu	1339	8	dual	dual	JJ
cord-014597-66vd2mdu	1339	9	(	(	-LRB-
cord-014597-66vd2mdu	1339	10	DsRed1	dsred1	JJ
cord-014597-66vd2mdu	1339	11	and	and	CC
cord-014597-66vd2mdu	1339	12	GFP	GFP	NNP
cord-014597-66vd2mdu	1339	13	)	)	-RRB-
cord-014597-66vd2mdu	1339	14	expression	expression	NN
cord-014597-66vd2mdu	1339	15	cassettes	cassette	NNS
cord-014597-66vd2mdu	1339	16	,	,	,
cord-014597-66vd2mdu	1339	17	were	be	VBD
cord-014597-66vd2mdu	1339	18	serially	serially	RB
cord-014597-66vd2mdu	1339	19	passaged	passage	VBN
cord-014597-66vd2mdu	1339	20	at	at	IN
cord-014597-66vd2mdu	1339	21	least	least	JJS
cord-014597-66vd2mdu	1339	22	20-fold	20-fold	CD
cord-014597-66vd2mdu	1339	23	.	.	.
cord-014597-66vd2mdu	1340	1	Although	although	IN
cord-014597-66vd2mdu	1340	2	the	the	DT
cord-014597-66vd2mdu	1340	3	genotype	genotype	NN
cord-014597-66vd2mdu	1340	4	of	of	IN
cord-014597-66vd2mdu	1340	5	MVA	MVA	NNP
cord-014597-66vd2mdu	1340	6	-	-	HYPH
cord-014597-66vd2mdu	1340	7	CR19	CR19	NNP
cord-014597-66vd2mdu	1340	8	is	be	VBZ
cord-014597-66vd2mdu	1340	9	advantageous	advantageous	JJ
cord-014597-66vd2mdu	1340	10	for	for	IN
cord-014597-66vd2mdu	1340	11	replication	replication	NN
cord-014597-66vd2mdu	1340	12	,	,	,
cord-014597-66vd2mdu	1340	13	all	all	DT
cord-014597-66vd2mdu	1340	14	genomic	genomic	JJ
cord-014597-66vd2mdu	1340	15	and	and	CC
cord-014597-66vd2mdu	1340	16	genetic	genetic	JJ
cord-014597-66vd2mdu	1340	17	markers	marker	NNS
cord-014597-66vd2mdu	1340	18	of	of	IN
cord-014597-66vd2mdu	1340	19	wildtype	wildtype	NN
cord-014597-66vd2mdu	1340	20	and	and	CC
cord-014597-66vd2mdu	1340	21	MVA	MVA	NNP
cord-014597-66vd2mdu	1340	22	-	-	HYPH
cord-014597-66vd2mdu	1340	23	CR19	CR19	NNP
cord-014597-66vd2mdu	1340	24	were	be	VBD
cord-014597-66vd2mdu	1340	25	stably	stably	RB
cord-014597-66vd2mdu	1340	26	maintained	maintain	VBN
cord-014597-66vd2mdu	1340	27	in	in	IN
cord-014597-66vd2mdu	1340	28	all	all	DT
cord-014597-66vd2mdu	1340	29	passages	passage	NNS
cord-014597-66vd2mdu	1340	30	of	of	IN
cord-014597-66vd2mdu	1340	31	the	the	DT
cord-014597-66vd2mdu	1340	32	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1340	33	viruses	virus	NNS
cord-014597-66vd2mdu	1340	34	,	,	,
cord-014597-66vd2mdu	1340	35	independent	independent	JJ
cord-014597-66vd2mdu	1340	36	of	of	IN
cord-014597-66vd2mdu	1340	37	wildtype	wildtype	NN
cord-014597-66vd2mdu	1340	38	or	or	CC
cord-014597-66vd2mdu	1340	39	MVA	MVA	NNP
cord-014597-66vd2mdu	1340	40	-	-	HYPH
cord-014597-66vd2mdu	1340	41	CR19	CR19	NNP
cord-014597-66vd2mdu	1340	42	backbone	backbone	NN
cord-014597-66vd2mdu	1340	43	.	.	.
cord-014597-66vd2mdu	1341	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1341	2	confirmed	confirm	VBD
cord-014597-66vd2mdu	1341	3	our	-PRON-	PRP$
cord-014597-66vd2mdu	1341	4	previous	previous	JJ
cord-014597-66vd2mdu	1341	5	results	result	NNS
cord-014597-66vd2mdu	1341	6	that	that	WDT
cord-014597-66vd2mdu	1341	7	suggested	suggest	VBD
cord-014597-66vd2mdu	1341	8	that	that	IN
cord-014597-66vd2mdu	1341	9	MVA	MVA	NNP
cord-014597-66vd2mdu	1341	10	-	-	HYPH
cord-014597-66vd2mdu	1341	11	CR19	CR19	NNP
cord-014597-66vd2mdu	1341	12	replicates	replicate	VBZ
cord-014597-66vd2mdu	1341	13	efficiently	efficiently	RB
cord-014597-66vd2mdu	1341	14	in	in	IN
cord-014597-66vd2mdu	1341	15	single	single	JJ
cord-014597-66vd2mdu	1341	16	-	-	HYPH
cord-014597-66vd2mdu	1341	17	cell	cell	NN
cord-014597-66vd2mdu	1341	18	suspensions	suspension	NNS
cord-014597-66vd2mdu	1341	19	and	and	CC
cord-014597-66vd2mdu	1341	20	were	be	VBD
cord-014597-66vd2mdu	1341	21	able	able	JJ
cord-014597-66vd2mdu	1341	22	to	to	TO
cord-014597-66vd2mdu	1341	23	connect	connect	VB
cord-014597-66vd2mdu	1341	24	this	this	DT
cord-014597-66vd2mdu	1341	25	property	property	NN
cord-014597-66vd2mdu	1341	26	with	with	IN
cord-014597-66vd2mdu	1341	27	the	the	DT
cord-014597-66vd2mdu	1341	28	D86Y	D86Y	NNP
cord-014597-66vd2mdu	1341	29	mutation	mutation	NN
cord-014597-66vd2mdu	1341	30	in	in	IN
cord-014597-66vd2mdu	1341	31	A34	A34	NNP
cord-014597-66vd2mdu	1341	32	,	,	,
cord-014597-66vd2mdu	1341	33	a	a	DT
cord-014597-66vd2mdu	1341	34	structural	structural	JJ
cord-014597-66vd2mdu	1341	35	protein	protein	NN
cord-014597-66vd2mdu	1341	36	on	on	IN
cord-014597-66vd2mdu	1341	37	the	the	DT
cord-014597-66vd2mdu	1341	38	surface	surface	NN
cord-014597-66vd2mdu	1341	39	of	of	IN
cord-014597-66vd2mdu	1341	40	the	the	DT
cord-014597-66vd2mdu	1341	41	virions	virion	NNS
cord-014597-66vd2mdu	1341	42	.	.	.
cord-014597-66vd2mdu	1342	1	MVA	MVA	NNP
cord-014597-66vd2mdu	1342	2	-	-	HYPH
cord-014597-66vd2mdu	1342	3	CR19	CR19	NNP
cord-014597-66vd2mdu	1342	4	was	be	VBD
cord-014597-66vd2mdu	1342	5	also	also	RB
cord-014597-66vd2mdu	1342	6	found	find	VBN
cord-014597-66vd2mdu	1342	7	to	to	TO
cord-014597-66vd2mdu	1342	8	differ	differ	VB
cord-014597-66vd2mdu	1342	9	from	from	IN
cord-014597-66vd2mdu	1342	10	wildtype	wildtype	NN
cord-014597-66vd2mdu	1342	11	MVA	MVA	NNP
cord-014597-66vd2mdu	1342	12	by	by	IN
cord-014597-66vd2mdu	1342	13	a	a	DT
cord-014597-66vd2mdu	1342	14	recombination	recombination	NN
cord-014597-66vd2mdu	1342	15	between	between	IN
cord-014597-66vd2mdu	1342	16	left	left	JJ
cord-014597-66vd2mdu	1342	17	and	and	CC
cord-014597-66vd2mdu	1342	18	right	right	JJ
cord-014597-66vd2mdu	1342	19	viral	viral	JJ
cord-014597-66vd2mdu	1342	20	telomere	telomere	NN
cord-014597-66vd2mdu	1342	21	.	.	.
cord-014597-66vd2mdu	1343	1	Due	due	IN
cord-014597-66vd2mdu	1343	2	to	to	IN
cord-014597-66vd2mdu	1343	3	this	this	DT
cord-014597-66vd2mdu	1343	4	event	event	NN
cord-014597-66vd2mdu	1343	5	,	,	,
cord-014597-66vd2mdu	1343	6	several	several	JJ
cord-014597-66vd2mdu	1343	7	genes	gene	NNS
cord-014597-66vd2mdu	1343	8	encoded	encode	VBN
cord-014597-66vd2mdu	1343	9	at	at	IN
cord-014597-66vd2mdu	1343	10	the	the	DT
cord-014597-66vd2mdu	1343	11	left	left	JJ
cord-014597-66vd2mdu	1343	12	terminus	terminus	NN
cord-014597-66vd2mdu	1343	13	have	have	VBP
cord-014597-66vd2mdu	1343	14	been	be	VBN
cord-014597-66vd2mdu	1343	15	deleted	delete	VBN
cord-014597-66vd2mdu	1343	16	whereas	whereas	IN
cord-014597-66vd2mdu	1343	17	the	the	DT
cord-014597-66vd2mdu	1343	18	gene	gene	NN
cord-014597-66vd2mdu	1343	19	dosis	dosis	NN
cord-014597-66vd2mdu	1343	20	of	of	IN
cord-014597-66vd2mdu	1343	21	those	those	DT
cord-014597-66vd2mdu	1343	22	originally	originally	RB
cord-014597-66vd2mdu	1343	23	encoded	encode	VBN
cord-014597-66vd2mdu	1343	24	only	only	RB
cord-014597-66vd2mdu	1343	25	at	at	IN
cord-014597-66vd2mdu	1343	26	the	the	DT
cord-014597-66vd2mdu	1343	27	right	right	JJ
cord-014597-66vd2mdu	1343	28	terminus	terminus	NN
cord-014597-66vd2mdu	1343	29	may	may	MD
cord-014597-66vd2mdu	1343	30	have	have	VB
cord-014597-66vd2mdu	1343	31	increased	increase	VBN
cord-014597-66vd2mdu	1343	32	.	.	.
cord-014597-66vd2mdu	1344	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1344	2	do	do	VBP
cord-014597-66vd2mdu	1344	3	not	not	RB
cord-014597-66vd2mdu	1344	4	currently	currently	RB
cord-014597-66vd2mdu	1344	5	know	know	VB
cord-014597-66vd2mdu	1344	6	how	how	WRB
cord-014597-66vd2mdu	1344	7	much	much	JJ
cord-014597-66vd2mdu	1344	8	the	the	DT
cord-014597-66vd2mdu	1344	9	various	various	JJ
cord-014597-66vd2mdu	1344	10	point	point	NN
cord-014597-66vd2mdu	1344	11	mutations	mutation	NNS
cord-014597-66vd2mdu	1344	12	and	and	CC
cord-014597-66vd2mdu	1344	13	changes	change	NNS
cord-014597-66vd2mdu	1344	14	in	in	IN
cord-014597-66vd2mdu	1344	15	genomic	genomic	JJ
cord-014597-66vd2mdu	1344	16	structure	structure	NN
cord-014597-66vd2mdu	1344	17	combine	combine	VBP
cord-014597-66vd2mdu	1344	18	to	to	TO
cord-014597-66vd2mdu	1344	19	explain	explain	VB
cord-014597-66vd2mdu	1344	20	the	the	DT
cord-014597-66vd2mdu	1344	21	improved	improved	JJ
cord-014597-66vd2mdu	1344	22	replication	replication	NN
cord-014597-66vd2mdu	1344	23	of	of	IN
cord-014597-66vd2mdu	1344	24	MVA	MVA	NNP
cord-014597-66vd2mdu	1344	25	-	-	HYPH
cord-014597-66vd2mdu	1344	26	CR19	CR19	NNP
cord-014597-66vd2mdu	1344	27	.	.	.
cord-014597-66vd2mdu	1345	1	As	as	IN
cord-014597-66vd2mdu	1345	2	several	several	JJ
cord-014597-66vd2mdu	1345	3	of	of	IN
cord-014597-66vd2mdu	1345	4	the	the	DT
cord-014597-66vd2mdu	1345	5	affected	affect	VBN
cord-014597-66vd2mdu	1345	6	genes	gene	NNS
cord-014597-66vd2mdu	1345	7	have	have	VBP
cord-014597-66vd2mdu	1345	8	been	be	VBN
cord-014597-66vd2mdu	1345	9	reported	report	VBN
cord-014597-66vd2mdu	1345	10	in	in	IN
cord-014597-66vd2mdu	1345	11	the	the	DT
cord-014597-66vd2mdu	1345	12	literature	literature	NN
cord-014597-66vd2mdu	1345	13	to	to	IN
cord-014597-66vd2mdu	1345	14	impact	impact	VB
cord-014597-66vd2mdu	1345	15	interaction	interaction	NN
cord-014597-66vd2mdu	1345	16	of	of	IN
cord-014597-66vd2mdu	1345	17	MVA	MVA	NNP
cord-014597-66vd2mdu	1345	18	with	with	IN
cord-014597-66vd2mdu	1345	19	the	the	DT
cord-014597-66vd2mdu	1345	20	host	host	NN
cord-014597-66vd2mdu	1345	21	we	-PRON-	PRP
cord-014597-66vd2mdu	1345	22	would	would	MD
cord-014597-66vd2mdu	1345	23	expect	expect	VB
cord-014597-66vd2mdu	1345	24	that	that	IN
cord-014597-66vd2mdu	1345	25	in	in	IN
cord-014597-66vd2mdu	1345	26	vivo	vivo	NN
cord-014597-66vd2mdu	1345	27	studies	study	NNS
cord-014597-66vd2mdu	1345	28	may	may	MD
cord-014597-66vd2mdu	1345	29	reveal	reveal	VB
cord-014597-66vd2mdu	1345	30	additional	additional	JJ
cord-014597-66vd2mdu	1345	31	novel	novel	JJ
cord-014597-66vd2mdu	1345	32	properties	property	NNS
cord-014597-66vd2mdu	1345	33	of	of	IN
cord-014597-66vd2mdu	1345	34	MVA	MVA	NNP
cord-014597-66vd2mdu	1345	35	-	-	HYPH
cord-014597-66vd2mdu	1345	36	CR19	CR19	NNP
cord-014597-66vd2mdu	1345	37	.	.	.
cord-014597-66vd2mdu	1346	1	An	an	DT
cord-014597-66vd2mdu	1346	2	extremely	extremely	RB
cord-014597-66vd2mdu	1346	3	important	important	JJ
cord-014597-66vd2mdu	1346	4	distinction	distinction	NN
cord-014597-66vd2mdu	1346	5	between	between	IN
cord-014597-66vd2mdu	1346	6	our	-PRON-	PRP$
cord-014597-66vd2mdu	1346	7	earlier	early	JJR
cord-014597-66vd2mdu	1346	8	study	study	NN
cord-014597-66vd2mdu	1346	9	[	[	-LRB-
cord-014597-66vd2mdu	1346	10	3	3	CD
cord-014597-66vd2mdu	1346	11	]	]	-RRB-
cord-014597-66vd2mdu	1346	12	and	and	CC
cord-014597-66vd2mdu	1346	13	this	this	DT
cord-014597-66vd2mdu	1346	14	one	one	CD
cord-014597-66vd2mdu	1346	15	concerns	concern	VBZ
cord-014597-66vd2mdu	1346	16	the	the	DT
cord-014597-66vd2mdu	1346	17	source	source	NN
cord-014597-66vd2mdu	1346	18	of	of	IN
cord-014597-66vd2mdu	1346	19	the	the	DT
cord-014597-66vd2mdu	1346	20	viruses	virus	NNS
cord-014597-66vd2mdu	1346	21	.	.	.
cord-014597-66vd2mdu	1347	1	Here	here	RB
cord-014597-66vd2mdu	1347	2	,	,	,
cord-014597-66vd2mdu	1347	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1347	4	investigated	investigate	VBD
cord-014597-66vd2mdu	1347	5	plaque	plaque	NN
cord-014597-66vd2mdu	1347	6	-	-	HYPH
cord-014597-66vd2mdu	1347	7	purified	purify	VBN
cord-014597-66vd2mdu	1347	8	viruses	virus	NNS
cord-014597-66vd2mdu	1347	9	and	and	CC
cord-014597-66vd2mdu	1347	10	confirm	confirm	VB
cord-014597-66vd2mdu	1347	11	the	the	DT
cord-014597-66vd2mdu	1347	12	high	high	JJ
cord-014597-66vd2mdu	1347	13	genetic	genetic	JJ
cord-014597-66vd2mdu	1347	14	and	and	CC
cord-014597-66vd2mdu	1347	15	genomic	genomic	JJ
cord-014597-66vd2mdu	1347	16	stability	stability	NN
cord-014597-66vd2mdu	1347	17	of	of	IN
cord-014597-66vd2mdu	1347	18	MVA	MVA	NNP
cord-014597-66vd2mdu	1347	19	.	.	.
cord-014597-66vd2mdu	1348	1	Different	different	JJ
cord-014597-66vd2mdu	1348	2	expression	expression	NN
cord-014597-66vd2mdu	1348	3	cassettes	cassette	NNS
cord-014597-66vd2mdu	1348	4	inserted	insert	VBN
cord-014597-66vd2mdu	1348	5	into	into	IN
cord-014597-66vd2mdu	1348	6	deletion	deletion	NN
cord-014597-66vd2mdu	1348	7	site	site	NN
cord-014597-66vd2mdu	1348	8	III	iii	CD
cord-014597-66vd2mdu	1348	9	,	,	,
cord-014597-66vd2mdu	1348	10	all	all	DT
cord-014597-66vd2mdu	1348	11	diagnostic	diagnostic	JJ
cord-014597-66vd2mdu	1348	12	RFLPs	rflp	NNS
cord-014597-66vd2mdu	1348	13	and	and	CC
cord-014597-66vd2mdu	1348	14	PCRs	pcr	NNS
cord-014597-66vd2mdu	1348	15	over	over	IN
cord-014597-66vd2mdu	1348	16	various	various	JJ
cord-014597-66vd2mdu	1348	17	sites	site	NNS
cord-014597-66vd2mdu	1348	18	of	of	IN
cord-014597-66vd2mdu	1348	19	the	the	DT
cord-014597-66vd2mdu	1348	20	genome	genome	NN
cord-014597-66vd2mdu	1348	21	and	and	CC
cord-014597-66vd2mdu	1348	22	within	within	IN
cord-014597-66vd2mdu	1348	23	the	the	DT
cord-014597-66vd2mdu	1348	24	viral	viral	JJ
cord-014597-66vd2mdu	1348	25	telomeres	telomere	NNS
cord-014597-66vd2mdu	1348	26	remained	remain	VBD
cord-014597-66vd2mdu	1348	27	unchanged	unchanged	JJ
cord-014597-66vd2mdu	1348	28	throughout	throughout	IN
cord-014597-66vd2mdu	1348	29	at	at	RB
cord-014597-66vd2mdu	1348	30	least	least	RBS
cord-014597-66vd2mdu	1348	31	20	20	CD
cord-014597-66vd2mdu	1348	32	serial	serial	JJ
cord-014597-66vd2mdu	1348	33	passages	passage	NNS
cord-014597-66vd2mdu	1348	34	-independent	-independent	JJ
cord-014597-66vd2mdu	1348	35	of	of	IN
cord-014597-66vd2mdu	1348	36	whether	whether	IN
cord-014597-66vd2mdu	1348	37	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1348	38	viruses	virus	NNS
cord-014597-66vd2mdu	1348	39	with	with	IN
cord-014597-66vd2mdu	1348	40	wildtype	wildtype	NN
cord-014597-66vd2mdu	1348	41	or	or	CC
cord-014597-66vd2mdu	1348	42	CR19-derived	cr19-derived	CD
cord-014597-66vd2mdu	1348	43	backbones	backbone	NNS
cord-014597-66vd2mdu	1348	44	were	be	VBD
cord-014597-66vd2mdu	1348	45	characterized	characterize	VBN
cord-014597-66vd2mdu	1348	46	.	.	.
cord-014597-66vd2mdu	1349	1	Fig	Fig	NNP
cord-014597-66vd2mdu	1349	2	.	.	.
cord-014597-66vd2mdu	1349	3	1	1	CD
cord-014597-66vd2mdu	1349	4	(	(	-LRB-
cord-014597-66vd2mdu	1349	5	abstract	abstract	JJ
cord-014597-66vd2mdu	1349	6	P-225	p-225	JJ
cord-014597-66vd2mdu	1349	7	)	)	-RRB-
cord-014597-66vd2mdu	1349	8	.	.	.
cord-014597-66vd2mdu	1350	1	a	a	DT
cord-014597-66vd2mdu	1350	2	One	one	CD
cord-014597-66vd2mdu	1350	3	hallmark	hallmark	NN
cord-014597-66vd2mdu	1350	4	of	of	IN
cord-014597-66vd2mdu	1350	5	MVA	MVA	NNP
cord-014597-66vd2mdu	1350	6	-	-	HYPH
cord-014597-66vd2mdu	1350	7	CR19	CR19	NNP
cord-014597-66vd2mdu	1350	8	is	be	VBZ
cord-014597-66vd2mdu	1350	9	a	a	DT
cord-014597-66vd2mdu	1350	10	significantly	significantly	RB
cord-014597-66vd2mdu	1350	11	reduced	reduce	VBN
cord-014597-66vd2mdu	1350	12	tendency	tendency	NN
cord-014597-66vd2mdu	1350	13	to	to	TO
cord-014597-66vd2mdu	1350	14	induce	induce	VB
cord-014597-66vd2mdu	1350	15	syncytia	syncytia	NN
cord-014597-66vd2mdu	1350	16	and	and	CC
cord-014597-66vd2mdu	1350	17	an	an	DT
cord-014597-66vd2mdu	1350	18	increased	increase	VBN
cord-014597-66vd2mdu	1350	19	dispersion	dispersion	NN
cord-014597-66vd2mdu	1350	20	of	of	IN
cord-014597-66vd2mdu	1350	21	plaques	plaque	NNS
cord-014597-66vd2mdu	1350	22	in	in	IN
cord-014597-66vd2mdu	1350	23	CR.pIX	CR.pIX	NNP
cord-014597-66vd2mdu	1350	24	cell	cell	NN
cord-014597-66vd2mdu	1350	25	monolayers	monolayer	NNS
cord-014597-66vd2mdu	1350	26	.	.	.
cord-014597-66vd2mdu	1351	1	This	this	DT
cord-014597-66vd2mdu	1351	2	property	property	NN
cord-014597-66vd2mdu	1351	3	appears	appear	VBZ
cord-014597-66vd2mdu	1351	4	to	to	TO
cord-014597-66vd2mdu	1351	5	be	be	VB
cord-014597-66vd2mdu	1351	6	supported	support	VBN
cord-014597-66vd2mdu	1351	7	by	by	IN
cord-014597-66vd2mdu	1351	8	the	the	DT
cord-014597-66vd2mdu	1351	9	mutation	mutation	NN
cord-014597-66vd2mdu	1351	10	in	in	IN
cord-014597-66vd2mdu	1351	11	A34R.	a34r.	NN
cord-014597-66vd2mdu	1351	12	b	b	NN
cord-014597-66vd2mdu	1351	13	Electron	electron	NN
cord-014597-66vd2mdu	1351	14	microscopy	microscopy	NN
cord-014597-66vd2mdu	1351	15	reveals	reveal	VBZ
cord-014597-66vd2mdu	1351	16	no	no	DT
cord-014597-66vd2mdu	1351	17	obvious	obvious	JJ
cord-014597-66vd2mdu	1351	18	differences	difference	NNS
cord-014597-66vd2mdu	1351	19	between	between	IN
cord-014597-66vd2mdu	1351	20	novel	novel	NN
cord-014597-66vd2mdu	1351	21	genotype	genotype	NN
cord-014597-66vd2mdu	1351	22	and	and	CC
cord-014597-66vd2mdu	1351	23	wildtype	wildtype	NN
cord-014597-66vd2mdu	1351	24	.	.	.
cord-014597-66vd2mdu	1352	1	Background	Background	NNP
cord-014597-66vd2mdu	1352	2	Transient	transient	JJ
cord-014597-66vd2mdu	1352	3	gene	gene	NN
cord-014597-66vd2mdu	1352	4	expression	expression	NN
cord-014597-66vd2mdu	1352	5	systems	system	NNS
cord-014597-66vd2mdu	1352	6	using	use	VBG
cord-014597-66vd2mdu	1352	7	polyethylenimine	polyethylenimine	NNP
cord-014597-66vd2mdu	1352	8	(	(	-LRB-
cord-014597-66vd2mdu	1352	9	PEI	PEI	NNP
cord-014597-66vd2mdu	1352	10	)	)	-RRB-
cord-014597-66vd2mdu	1352	11	are	be	VBP
cord-014597-66vd2mdu	1352	12	considered	consider	VBN
cord-014597-66vd2mdu	1352	13	to	to	TO
cord-014597-66vd2mdu	1352	14	be	be	VB
cord-014597-66vd2mdu	1352	15	fast	fast	RB
cord-014597-66vd2mdu	1352	16	,	,	,
cord-014597-66vd2mdu	1352	17	flexible	flexible	JJ
cord-014597-66vd2mdu	1352	18	and	and	CC
cord-014597-66vd2mdu	1352	19	cost	cost	NN
cord-014597-66vd2mdu	1352	20	-	-	HYPH
cord-014597-66vd2mdu	1352	21	efficient	efficient	JJ
cord-014597-66vd2mdu	1352	22	for	for	IN
cord-014597-66vd2mdu	1352	23	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1352	24	protein	protein	NN
cord-014597-66vd2mdu	1352	25	production	production	NN
cord-014597-66vd2mdu	1352	26	[	[	-LRB-
cord-014597-66vd2mdu	1352	27	1	1	CD
cord-014597-66vd2mdu	1352	28	]	]	-RRB-
cord-014597-66vd2mdu	1352	29	.	.	.
cord-014597-66vd2mdu	1353	1	Transfection	transfection	NN
cord-014597-66vd2mdu	1353	2	efficiency	efficiency	NN
cord-014597-66vd2mdu	1353	3	depends	depend	VBZ
cord-014597-66vd2mdu	1353	4	on	on	IN
cord-014597-66vd2mdu	1353	5	different	different	JJ
cord-014597-66vd2mdu	1353	6	factors	factor	NNS
cord-014597-66vd2mdu	1353	7	;	;	:
cord-014597-66vd2mdu	1353	8	one	one	CD
cord-014597-66vd2mdu	1353	9	of	of	IN
cord-014597-66vd2mdu	1353	10	them	-PRON-	PRP
cord-014597-66vd2mdu	1353	11	is	be	VBZ
cord-014597-66vd2mdu	1353	12	the	the	DT
cord-014597-66vd2mdu	1353	13	type	type	NN
cord-014597-66vd2mdu	1353	14	of	of	IN
cord-014597-66vd2mdu	1353	15	media	medium	NNS
cord-014597-66vd2mdu	1353	16	.	.	.
cord-014597-66vd2mdu	1354	1	Production	production	NN
cord-014597-66vd2mdu	1354	2	media	medium	NNS
cord-014597-66vd2mdu	1354	3	support	support	NN
cord-014597-66vd2mdu	1354	4	cell	cell	NN
cord-014597-66vd2mdu	1354	5	growth	growth	NN
cord-014597-66vd2mdu	1354	6	and	and	CC
cord-014597-66vd2mdu	1354	7	protein	protein	NN
cord-014597-66vd2mdu	1354	8	production	production	NN
cord-014597-66vd2mdu	1354	9	but	but	CC
cord-014597-66vd2mdu	1354	10	not	not	RB
cord-014597-66vd2mdu	1354	11	high	high	JJ
cord-014597-66vd2mdu	1354	12	transfection	transfection	NN
cord-014597-66vd2mdu	1354	13	efficiency	efficiency	NN
cord-014597-66vd2mdu	1354	14	(	(	-LRB-
cord-014597-66vd2mdu	1354	15	TE	TE	NNP
cord-014597-66vd2mdu	1354	16	)	)	-RRB-
cord-014597-66vd2mdu	1354	17	mediated	mediate	VBN
cord-014597-66vd2mdu	1354	18	by	by	IN
cord-014597-66vd2mdu	1354	19	PEI	PEI	NNP
cord-014597-66vd2mdu	1354	20	[	[	-LRB-
cord-014597-66vd2mdu	1354	21	2	2	CD
cord-014597-66vd2mdu	1354	22	]	]	-RRB-
cord-014597-66vd2mdu	1354	23	.	.	.
cord-014597-66vd2mdu	1355	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1355	2	,	,	,
cord-014597-66vd2mdu	1355	3	media	medium	NNS
cord-014597-66vd2mdu	1355	4	were	be	VBD
cord-014597-66vd2mdu	1355	5	selected	select	VBN
cord-014597-66vd2mdu	1355	6	for	for	IN
cord-014597-66vd2mdu	1355	7	transfection	transfection	NN
cord-014597-66vd2mdu	1355	8	followed	follow	VBN
cord-014597-66vd2mdu	1355	9	by	by	IN
cord-014597-66vd2mdu	1355	10	feeding	feed	VBG
cord-014597-66vd2mdu	1355	11	of	of	IN
cord-014597-66vd2mdu	1355	12	production	production	NN
cord-014597-66vd2mdu	1355	13	media	medium	NNS
cord-014597-66vd2mdu	1355	14	[	[	-LRB-
cord-014597-66vd2mdu	1355	15	3	3	CD
cord-014597-66vd2mdu	1355	16	]	]	-RRB-
cord-014597-66vd2mdu	1355	17	to	to	TO
cord-014597-66vd2mdu	1355	18	improve	improve	VB
cord-014597-66vd2mdu	1355	19	TE	TE	NNP
cord-014597-66vd2mdu	1355	20	and	and	CC
cord-014597-66vd2mdu	1355	21	protein	protein	NN
cord-014597-66vd2mdu	1355	22	production	production	NN
cord-014597-66vd2mdu	1355	23	.	.	.
cord-014597-66vd2mdu	1356	1	Two	two	CD
cord-014597-66vd2mdu	1356	2	different	different	JJ
cord-014597-66vd2mdu	1356	3	transfection	transfection	NN
cord-014597-66vd2mdu	1356	4	strategies	strategy	NNS
cord-014597-66vd2mdu	1356	5	are	be	VBP
cord-014597-66vd2mdu	1356	6	compared	compare	VBN
cord-014597-66vd2mdu	1356	7	:	:	:
cord-014597-66vd2mdu	1356	8	conventional	conventional	JJ
cord-014597-66vd2mdu	1356	9	transfection	transfection	NN
cord-014597-66vd2mdu	1356	10	by	by	IN
cord-014597-66vd2mdu	1356	11	preparing	prepare	VBG
cord-014597-66vd2mdu	1356	12	polyplex	polyplex	NN
cord-014597-66vd2mdu	1356	13	of	of	IN
cord-014597-66vd2mdu	1356	14	a	a	DT
cord-014597-66vd2mdu	1356	15	plasmid	plasmid	NN
cord-014597-66vd2mdu	1356	16	(	(	-LRB-
cord-014597-66vd2mdu	1356	17	pDNA	pdna	RB
cord-014597-66vd2mdu	1356	18	)	)	-RRB-
cord-014597-66vd2mdu	1356	19	and	and	CC
cord-014597-66vd2mdu	1356	20	PEI	PEI	NNP
cord-014597-66vd2mdu	1356	21	interaction	interaction	NN
cord-014597-66vd2mdu	1356	22	before	before	IN
cord-014597-66vd2mdu	1356	23	transfection	transfection	NN
cord-014597-66vd2mdu	1356	24	and	and	CC
cord-014597-66vd2mdu	1356	25	insitu	insitu	JJ
cord-014597-66vd2mdu	1356	26	transfection	transfection	NN
cord-014597-66vd2mdu	1356	27	by	by	IN
cord-014597-66vd2mdu	1356	28	direct	direct	JJ
cord-014597-66vd2mdu	1356	29	addition	addition	NN
cord-014597-66vd2mdu	1356	30	both	both	DT
cord-014597-66vd2mdu	1356	31	of	of	IN
cord-014597-66vd2mdu	1356	32	them	-PRON-	PRP
cord-014597-66vd2mdu	1356	33	to	to	IN
cord-014597-66vd2mdu	1356	34	the	the	DT
cord-014597-66vd2mdu	1356	35	cell	cell	NN
cord-014597-66vd2mdu	1356	36	suspension	suspension	NN
cord-014597-66vd2mdu	1356	37	and	and	CC
cord-014597-66vd2mdu	1356	38	the	the	DT
cord-014597-66vd2mdu	1356	39	polyplex	polyplex	NN
cord-014597-66vd2mdu	1356	40	formed	form	VBN
cord-014597-66vd2mdu	1356	41	spontaneously	spontaneously	RB
cord-014597-66vd2mdu	1356	42	[	[	-LRB-
cord-014597-66vd2mdu	1356	43	4	4	CD
cord-014597-66vd2mdu	1356	44	]	]	-RRB-
cord-014597-66vd2mdu	1356	45	.	.	.
cord-014597-66vd2mdu	1357	1	Cells	cell	NNS
cord-014597-66vd2mdu	1357	2	were	be	VBD
cord-014597-66vd2mdu	1357	3	seeded	seed	VBN
cord-014597-66vd2mdu	1357	4	24	24	CD
cord-014597-66vd2mdu	1357	5	hr	hr	NN
cord-014597-66vd2mdu	1357	6	in	in	IN
cord-014597-66vd2mdu	1357	7	CHOMACS	CHOMACS	NNP
cord-014597-66vd2mdu	1357	8	CD	CD	NNP
cord-014597-66vd2mdu	1357	9	media	medium	NNS
cord-014597-66vd2mdu	1357	10	before	before	IN
cord-014597-66vd2mdu	1357	11	transfection	transfection	NN
cord-014597-66vd2mdu	1357	12	.	.	.
cord-014597-66vd2mdu	1358	1	At	at	IN
cord-014597-66vd2mdu	1358	2	transfection	transfection	NN
cord-014597-66vd2mdu	1358	3	time	time	NN
cord-014597-66vd2mdu	1358	4	point	point	VBP
cord-014597-66vd2mdu	1358	5	an	an	DT
cord-014597-66vd2mdu	1358	6	equal	equal	JJ
cord-014597-66vd2mdu	1358	7	amount	amount	NN
cord-014597-66vd2mdu	1358	8	of	of	IN
cord-014597-66vd2mdu	1358	9	cells	cell	NNS
cord-014597-66vd2mdu	1358	10	were	be	VBD
cord-014597-66vd2mdu	1358	11	resuspended	resuspend	VBN
cord-014597-66vd2mdu	1358	12	in	in	IN
cord-014597-66vd2mdu	1358	13	each	each	DT
cord-014597-66vd2mdu	1358	14	media	medium	NNS
cord-014597-66vd2mdu	1358	15	type	type	NN
cord-014597-66vd2mdu	1358	16	.	.	.
cord-014597-66vd2mdu	1359	1	Transfection	Transfection	NNP
cord-014597-66vd2mdu	1359	2	was	be	VBD
cord-014597-66vd2mdu	1359	3	applied	apply	VBN
cord-014597-66vd2mdu	1359	4	either	either	CC
cord-014597-66vd2mdu	1359	5	insitu	insitu	JJ
cord-014597-66vd2mdu	1359	6	or	or	CC
cord-014597-66vd2mdu	1359	7	conventional	conventional	JJ
cord-014597-66vd2mdu	1360	1	(	(	-LRB-
cord-014597-66vd2mdu	1360	2	polyplex	polyplex	NNP
cord-014597-66vd2mdu	1360	3	prepared	prepare	VBD
cord-014597-66vd2mdu	1360	4	in	in	IN
cord-014597-66vd2mdu	1360	5	100	100	CD
cord-014597-66vd2mdu	1360	6	μL	μL	NNS
cord-014597-66vd2mdu	1360	7	of	of	IN
cord-014597-66vd2mdu	1360	8	150	150	CD
cord-014597-66vd2mdu	1360	9	mM	mm	CD
cord-014597-66vd2mdu	1360	10	NaCl	nacl	NN
cord-014597-66vd2mdu	1360	11	and	and	CC
cord-014597-66vd2mdu	1360	12	incubated	incubate	VBN
cord-014597-66vd2mdu	1360	13	for	for	IN
cord-014597-66vd2mdu	1360	14	20	20	CD
cord-014597-66vd2mdu	1360	15	min	min	NN
cord-014597-66vd2mdu	1360	16	.	.	.
cord-014597-66vd2mdu	1360	17	)	)	-RRB-
cord-014597-66vd2mdu	1360	18	,	,	,
cord-014597-66vd2mdu	1360	19	media	medium	NNS
cord-014597-66vd2mdu	1360	20	addition	addition	NN
cord-014597-66vd2mdu	1360	21	was	be	VBD
cord-014597-66vd2mdu	1360	22	performed	perform	VBN
cord-014597-66vd2mdu	1360	23	5	5	CD
cord-014597-66vd2mdu	1360	24	hours	hour	NNS
cord-014597-66vd2mdu	1360	25	post	post	NN
cord-014597-66vd2mdu	1360	26	-	-	NN
cord-014597-66vd2mdu	1360	27	transfection	transfection	JJ
cord-014597-66vd2mdu	1360	28	(	(	-LRB-
cord-014597-66vd2mdu	1360	29	hpt	hpt	NNP
cord-014597-66vd2mdu	1360	30	)	)	-RRB-
cord-014597-66vd2mdu	1360	31	.	.	.
cord-014597-66vd2mdu	1361	1	Media	media	NN
cord-014597-66vd2mdu	1361	2	type	type	NN
cord-014597-66vd2mdu	1361	3	and	and	CC
cord-014597-66vd2mdu	1361	4	transfection	transfection	NN
cord-014597-66vd2mdu	1361	5	condition	condition	NN
cord-014597-66vd2mdu	1361	6	were	be	VBD
cord-014597-66vd2mdu	1361	7	illustrated	illustrate	VBN
cord-014597-66vd2mdu	1361	8	in	in	IN
cord-014597-66vd2mdu	1361	9	Table	Table	NNP
cord-014597-66vd2mdu	1361	10	1	1	CD
cord-014597-66vd2mdu	1361	11	.	.	.
cord-014597-66vd2mdu	1362	1	Media	medium	NNS
cord-014597-66vd2mdu	1362	2	screen	screen	NN
cord-014597-66vd2mdu	1362	3	result	result	NN
cord-014597-66vd2mdu	1362	4	exhibits	exhibit	VBZ
cord-014597-66vd2mdu	1362	5	the	the	DT
cord-014597-66vd2mdu	1362	6	highest	high	JJS
cord-014597-66vd2mdu	1362	7	transfection	transfection	NN
cord-014597-66vd2mdu	1362	8	efficiency	efficiency	NN
cord-014597-66vd2mdu	1362	9	of	of	IN
cord-014597-66vd2mdu	1362	10	around	around	IN
cord-014597-66vd2mdu	1362	11	50	50	CD
cord-014597-66vd2mdu	1362	12	%	%	NN
cord-014597-66vd2mdu	1362	13	transfected	transfecte	VBN
cord-014597-66vd2mdu	1362	14	cells	cell	NNS
cord-014597-66vd2mdu	1362	15	by	by	IN
cord-014597-66vd2mdu	1362	16	Opti	Opti	NNP
cord-014597-66vd2mdu	1362	17	-	-	HYPH
cord-014597-66vd2mdu	1362	18	MEM	mem	NN
cord-014597-66vd2mdu	1362	19	medium	medium	NN
cord-014597-66vd2mdu	1362	20	coming	come	VBG
cord-014597-66vd2mdu	1362	21	along	along	RB
cord-014597-66vd2mdu	1362	22	with	with	IN
cord-014597-66vd2mdu	1362	23	low	low	JJ
cord-014597-66vd2mdu	1362	24	cell	cell	NN
cord-014597-66vd2mdu	1362	25	growth	growth	NN
cord-014597-66vd2mdu	1362	26	and	and	CC
cord-014597-66vd2mdu	1362	27	viability	viability	NN
cord-014597-66vd2mdu	1362	28	.	.	.
cord-014597-66vd2mdu	1363	1	To	to	TO
cord-014597-66vd2mdu	1363	2	improve	improve	VB
cord-014597-66vd2mdu	1363	3	the	the	DT
cord-014597-66vd2mdu	1363	4	transfection	transfection	NN
cord-014597-66vd2mdu	1363	5	efficiency	efficiency	NN
cord-014597-66vd2mdu	1363	6	,	,	,
cord-014597-66vd2mdu	1363	7	basic	basic	JJ
cord-014597-66vd2mdu	1363	8	parameters	parameter	NNS
cord-014597-66vd2mdu	1363	9	including	include	VBG
cord-014597-66vd2mdu	1363	10	cell	cell	NN
cord-014597-66vd2mdu	1363	11	density	density	NN
cord-014597-66vd2mdu	1363	12	,	,	,
cord-014597-66vd2mdu	1363	13	pDNA	pdna	RB
cord-014597-66vd2mdu	1363	14	,	,	,
cord-014597-66vd2mdu	1363	15	and	and	CC
cord-014597-66vd2mdu	1363	16	PEI	PEI	NNP
cord-014597-66vd2mdu	1363	17	concentrations	concentration	NNS
cord-014597-66vd2mdu	1363	18	were	be	VBD
cord-014597-66vd2mdu	1363	19	varied	varied	JJ
cord-014597-66vd2mdu	1363	20	and	and	CC
cord-014597-66vd2mdu	1363	21	higher	high	JJR
cord-014597-66vd2mdu	1363	22	transfection	transfection	NN
cord-014597-66vd2mdu	1363	23	efficiency	efficiency	NN
cord-014597-66vd2mdu	1363	24	was	be	VBD
cord-014597-66vd2mdu	1363	25	reached	reach	VBN
cord-014597-66vd2mdu	1363	26	by	by	IN
cord-014597-66vd2mdu	1363	27	reducing	reduce	VBG
cord-014597-66vd2mdu	1363	28	media	medium	NNS
cord-014597-66vd2mdu	1363	29	or	or	CC
cord-014597-66vd2mdu	1363	30	accordingly	accordingly	RB
cord-014597-66vd2mdu	1363	31	increasing	increase	VBG
cord-014597-66vd2mdu	1363	32	cell	cell	NN
cord-014597-66vd2mdu	1363	33	density	density	NN
cord-014597-66vd2mdu	1363	34	,	,	,
cord-014597-66vd2mdu	1363	35	PEI	PEI	NNP
cord-014597-66vd2mdu	1363	36	and	and	CC
cord-014597-66vd2mdu	1363	37	pDNA	pdna	JJ
cord-014597-66vd2mdu	1363	38	concentration	concentration	NN
cord-014597-66vd2mdu	1363	39	for	for	IN
cord-014597-66vd2mdu	1363	40	transfection	transfection	NN
cord-014597-66vd2mdu	1363	41	.	.	.
cord-014597-66vd2mdu	1364	1	Further	further	JJ
cord-014597-66vd2mdu	1364	2	optimization	optimization	NN
cord-014597-66vd2mdu	1364	3	results	result	NNS
cord-014597-66vd2mdu	1364	4	show	show	VBP
cord-014597-66vd2mdu	1364	5	that	that	IN
cord-014597-66vd2mdu	1364	6	the	the	DT
cord-014597-66vd2mdu	1364	7	transfection	transfection	NN
cord-014597-66vd2mdu	1364	8	of	of	IN
cord-014597-66vd2mdu	1364	9	CHO	CHO	NNP
cord-014597-66vd2mdu	1364	10	-	-	HYPH
cord-014597-66vd2mdu	1364	11	K1	K1	NNP
cord-014597-66vd2mdu	1364	12	cells	cell	NNS
cord-014597-66vd2mdu	1364	13	in	in	IN
cord-014597-66vd2mdu	1364	14	Opti	Opti	NNP
cord-014597-66vd2mdu	1364	15	-	-	HYPH
cord-014597-66vd2mdu	1364	16	MEM	mem	NN
cord-014597-66vd2mdu	1364	17	(	(	-LRB-
cord-014597-66vd2mdu	1364	18	transfection	transfection	NN
cord-014597-66vd2mdu	1364	19	medium	medium	NN
cord-014597-66vd2mdu	1364	20	)	)	-RRB-
cord-014597-66vd2mdu	1364	21	for	for	IN
cord-014597-66vd2mdu	1364	22	5	5	CD
cord-014597-66vd2mdu	1364	23	hours	hour	NNS
cord-014597-66vd2mdu	1364	24	followed	follow	VBN
cord-014597-66vd2mdu	1364	25	by	by	IN
cord-014597-66vd2mdu	1364	26	addition	addition	NN
cord-014597-66vd2mdu	1364	27	of	of	IN
cord-014597-66vd2mdu	1364	28	CHO	CHO	NNP
cord-014597-66vd2mdu	1364	29	-	-	HYPH
cord-014597-66vd2mdu	1364	30	MACS	MACS	NNP
cord-014597-66vd2mdu	1364	31	CD	cd	NN
cord-014597-66vd2mdu	1364	32	(	(	-LRB-
cord-014597-66vd2mdu	1364	33	production	production	NN
cord-014597-66vd2mdu	1364	34	medium	medium	NN
cord-014597-66vd2mdu	1364	35	)	)	-RRB-
cord-014597-66vd2mdu	1364	36	for	for	IN
cord-014597-66vd2mdu	1364	37	further	further	RB
cord-014597-66vd2mdu	1364	38	enhancing	enhance	VBG
cord-014597-66vd2mdu	1364	39	the	the	DT
cord-014597-66vd2mdu	1364	40	transfection	transfection	NN
cord-014597-66vd2mdu	1364	41	,	,	,
cord-014597-66vd2mdu	1364	42	cell	cell	NN
cord-014597-66vd2mdu	1364	43	count	count	NN
cord-014597-66vd2mdu	1364	44	,	,	,
cord-014597-66vd2mdu	1364	45	and	and	CC
cord-014597-66vd2mdu	1364	46	cell	cell	NN
cord-014597-66vd2mdu	1364	47	viability	viability	NN
cord-014597-66vd2mdu	1364	48	.	.	.
cord-014597-66vd2mdu	1365	1	The	the	DT
cord-014597-66vd2mdu	1365	2	transfection	transfection	NN
cord-014597-66vd2mdu	1365	3	efficiency	efficiency	NN
cord-014597-66vd2mdu	1365	4	(	(	-LRB-
cord-014597-66vd2mdu	1365	5	TE	TE	NNP
cord-014597-66vd2mdu	1365	6	)	)	-RRB-
cord-014597-66vd2mdu	1365	7	increased	increase	VBD
cord-014597-66vd2mdu	1365	8	up	up	IN
cord-014597-66vd2mdu	1365	9	to	to	IN
cord-014597-66vd2mdu	1365	10	85	85	CD
cord-014597-66vd2mdu	1365	11	±	±	CD
cord-014597-66vd2mdu	1365	12	2.6	2.6	CD
cord-014597-66vd2mdu	1365	13	%	%	NN
cord-014597-66vd2mdu	1365	14	coincide	coincide	NN
cord-014597-66vd2mdu	1365	15	with	with	IN
cord-014597-66vd2mdu	1365	16	increases	increase	NNS
cord-014597-66vd2mdu	1365	17	in	in	IN
cord-014597-66vd2mdu	1365	18	viable	viable	JJ
cord-014597-66vd2mdu	1365	19	cell	cell	NN
cord-014597-66vd2mdu	1365	20	concentration	concentration	NN
cord-014597-66vd2mdu	1365	21	(	(	-LRB-
cord-014597-66vd2mdu	1365	22	VCC	VCC	NNP
cord-014597-66vd2mdu	1365	23	)	)	-RRB-
cord-014597-66vd2mdu	1365	24	in	in	IN
cord-014597-66vd2mdu	1365	25	comparsion	comparsion	NN
cord-014597-66vd2mdu	1365	26	to	to	IN
cord-014597-66vd2mdu	1365	27	transfection	transfection	NN
cord-014597-66vd2mdu	1365	28	and	and	CC
cord-014597-66vd2mdu	1365	29	cultivation	cultivation	NN
cord-014597-66vd2mdu	1365	30	in	in	IN
cord-014597-66vd2mdu	1365	31	Opti	opti	JJ
cord-014597-66vd2mdu	1365	32	-	-	HYPH
cord-014597-66vd2mdu	1365	33	MEM	mem	NN
cord-014597-66vd2mdu	1365	34	media	medium	NNS
cord-014597-66vd2mdu	1365	35	alone	alone	RB
cord-014597-66vd2mdu	1366	1	Fig	Fig	NNP
cord-014597-66vd2mdu	1366	2	.	.	.
cord-014597-66vd2mdu	1366	3	1a	1a	CD
cord-014597-66vd2mdu	1366	4	.	.	.
cord-014597-66vd2mdu	1367	1	Both	both	DT
cord-014597-66vd2mdu	1367	2	conventional	conventional	JJ
cord-014597-66vd2mdu	1367	3	and	and	CC
cord-014597-66vd2mdu	1367	4	insitu	insitu	JJ
cord-014597-66vd2mdu	1367	5	methods	method	NNS
cord-014597-66vd2mdu	1367	6	are	be	VBP
cord-014597-66vd2mdu	1367	7	successfully	successfully	RB
cord-014597-66vd2mdu	1367	8	transfected	transfecte	VBN
cord-014597-66vd2mdu	1367	9	CHO	CHO	NNP
cord-014597-66vd2mdu	1367	10	-	-	HYPH
cord-014597-66vd2mdu	1367	11	K1	K1	NNP
cord-014597-66vd2mdu	1367	12	to	to	IN
cord-014597-66vd2mdu	1367	13	the	the	DT
cord-014597-66vd2mdu	1367	14	same	same	JJ
cord-014597-66vd2mdu	1367	15	similar	similar	JJ
cord-014597-66vd2mdu	1367	16	high	high	JJ
cord-014597-66vd2mdu	1367	17	TE	TE	NNP
cord-014597-66vd2mdu	1367	18	as	as	IN
cord-014597-66vd2mdu	1367	19	shown	show	VBN
cord-014597-66vd2mdu	1367	20	in	in	IN
cord-014597-66vd2mdu	1367	21	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	1367	22	microscope	microscope	NN
cord-014597-66vd2mdu	1367	23	images	image	NNS
cord-014597-66vd2mdu	1367	24	of	of	IN
cord-014597-66vd2mdu	1367	25	Fig	Fig	NNP
cord-014597-66vd2mdu	1367	26	.	.	.
cord-014597-66vd2mdu	1368	1	1b	1b	LS
cord-014597-66vd2mdu	1368	2	.	.	.
cord-014597-66vd2mdu	1369	1	Insitu	Insitu	NNP
cord-014597-66vd2mdu	1369	2	transfection	transfection	NN
cord-014597-66vd2mdu	1369	3	shows	show	VBZ
cord-014597-66vd2mdu	1369	4	super	super	JJ
cord-014597-66vd2mdu	1369	5	-	-	NN
cord-014597-66vd2mdu	1369	6	priority	priority	NN
cord-014597-66vd2mdu	1369	7	for	for	IN
cord-014597-66vd2mdu	1369	8	suspension	suspension	NN
cord-014597-66vd2mdu	1369	9	cell	cell	NN
cord-014597-66vd2mdu	1369	10	transfection	transfection	NN
cord-014597-66vd2mdu	1369	11	concerning	concern	VBG
cord-014597-66vd2mdu	1369	12	the	the	DT
cord-014597-66vd2mdu	1369	13	reduction	reduction	NN
cord-014597-66vd2mdu	1369	14	of	of	IN
cord-014597-66vd2mdu	1369	15	handling	handling	NN
cord-014597-66vd2mdu	1369	16	steps	step	NNS
cord-014597-66vd2mdu	1369	17	(	(	-LRB-
cord-014597-66vd2mdu	1369	18	one	one	CD
cord-014597-66vd2mdu	1369	19	step	step	NN
cord-014597-66vd2mdu	1369	20	)	)	-RRB-
cord-014597-66vd2mdu	1369	21	compared	compare	VBN
cord-014597-66vd2mdu	1369	22	to	to	IN
cord-014597-66vd2mdu	1369	23	the	the	DT
cord-014597-66vd2mdu	1369	24	conventional	conventional	JJ
cord-014597-66vd2mdu	1369	25	way	way	NN
cord-014597-66vd2mdu	1369	26	(	(	-LRB-
cord-014597-66vd2mdu	1369	27	two	two	CD
cord-014597-66vd2mdu	1369	28	steps	step	NNS
cord-014597-66vd2mdu	1369	29	)	)	-RRB-
cord-014597-66vd2mdu	1369	30	.	.	.
cord-014597-66vd2mdu	1370	1	The	the	DT
cord-014597-66vd2mdu	1370	2	insitu	insitu	JJ
cord-014597-66vd2mdu	1370	3	transfection	transfection	NN
cord-014597-66vd2mdu	1370	4	avoiding	avoid	VBG
cord-014597-66vd2mdu	1370	5	the	the	DT
cord-014597-66vd2mdu	1370	6	optimization	optimization	NN
cord-014597-66vd2mdu	1370	7	step	step	NN
cord-014597-66vd2mdu	1370	8	required	require	VBN
cord-014597-66vd2mdu	1370	9	for	for	IN
cord-014597-66vd2mdu	1370	10	the	the	DT
cord-014597-66vd2mdu	1370	11	incubation	incubation	NN
cord-014597-66vd2mdu	1370	12	period	period	NN
cord-014597-66vd2mdu	1370	13	to	to	TO
cord-014597-66vd2mdu	1370	14	prepare	prepare	VB
cord-014597-66vd2mdu	1370	15	transfection	transfection	NN
cord-014597-66vd2mdu	1370	16	polyplex	polyplex	NN
cord-014597-66vd2mdu	1370	17	but	but	CC
cord-014597-66vd2mdu	1370	18	require	require	VBP
cord-014597-66vd2mdu	1370	19	a	a	DT
cord-014597-66vd2mdu	1370	20	higher	high	JJR
cord-014597-66vd2mdu	1370	21	amount	amount	NN
cord-014597-66vd2mdu	1370	22	of	of	IN
cord-014597-66vd2mdu	1370	23	pDNA	pdna	NN
cord-014597-66vd2mdu	1370	24	and	and	CC
cord-014597-66vd2mdu	1370	25	PEI	PEI	NNP
cord-014597-66vd2mdu	1370	26	than	than	IN
cord-014597-66vd2mdu	1370	27	conventional	conventional	JJ
cord-014597-66vd2mdu	1370	28	way	way	NN
cord-014597-66vd2mdu	1370	29	as	as	IN
cord-014597-66vd2mdu	1370	30	shown	show	VBN
cord-014597-66vd2mdu	1370	31	in	in	IN
cord-014597-66vd2mdu	1370	32	Table	Table	NNP
cord-014597-66vd2mdu	1370	33	1	1	CD
cord-014597-66vd2mdu	1370	34	.	.	.
cord-014597-66vd2mdu	1371	1	In	in	IN
cord-014597-66vd2mdu	1371	2	order	order	NN
cord-014597-66vd2mdu	1371	3	to	to	TO
cord-014597-66vd2mdu	1371	4	deal	deal	VB
cord-014597-66vd2mdu	1371	5	with	with	IN
cord-014597-66vd2mdu	1371	6	the	the	DT
cord-014597-66vd2mdu	1371	7	growing	grow	VBG
cord-014597-66vd2mdu	1371	8	demand	demand	NN
cord-014597-66vd2mdu	1371	9	of	of	IN
cord-014597-66vd2mdu	1371	10	large	large	JJ
cord-014597-66vd2mdu	1371	11	quantities	quantity	NNS
cord-014597-66vd2mdu	1371	12	of	of	IN
cord-014597-66vd2mdu	1371	13	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1371	14	proteins	protein	NNS
cord-014597-66vd2mdu	1371	15	in	in	IN
cord-014597-66vd2mdu	1371	16	a	a	DT
cord-014597-66vd2mdu	1371	17	timely	timely	JJ
cord-014597-66vd2mdu	1371	18	fashion	fashion	NN
cord-014597-66vd2mdu	1371	19	,	,	,
cord-014597-66vd2mdu	1371	20	expression	expression	NN
cord-014597-66vd2mdu	1371	21	systems	system	NNS
cord-014597-66vd2mdu	1371	22	are	be	VBP
cord-014597-66vd2mdu	1371	23	being	be	VBG
cord-014597-66vd2mdu	1371	24	optimized	optimize	VBN
cord-014597-66vd2mdu	1371	25	to	to	TO
cord-014597-66vd2mdu	1371	26	reduce	reduce	VB
cord-014597-66vd2mdu	1371	27	the	the	DT
cord-014597-66vd2mdu	1371	28	time	time	NN
cord-014597-66vd2mdu	1371	29	of	of	IN
cord-014597-66vd2mdu	1371	30	generation	generation	NN
cord-014597-66vd2mdu	1371	31	of	of	IN
cord-014597-66vd2mdu	1371	32	stable	stable	JJ
cord-014597-66vd2mdu	1371	33	clones	clone	NNS
cord-014597-66vd2mdu	1371	34	as	as	RB
cord-014597-66vd2mdu	1371	35	well	well	RB
cord-014597-66vd2mdu	1371	36	as	as	IN
cord-014597-66vd2mdu	1371	37	to	to	TO
cord-014597-66vd2mdu	1371	38	increase	increase	VB
cord-014597-66vd2mdu	1371	39	the	the	DT
cord-014597-66vd2mdu	1371	40	levels	level	NNS
cord-014597-66vd2mdu	1371	41	of	of	IN
cord-014597-66vd2mdu	1371	42	protein	protein	NN
cord-014597-66vd2mdu	1371	43	secretion	secretion	NN
cord-014597-66vd2mdu	1371	44	.	.	.
cord-014597-66vd2mdu	1372	1	This	this	DT
cord-014597-66vd2mdu	1372	2	can	can	MD
cord-014597-66vd2mdu	1372	3	be	be	VB
cord-014597-66vd2mdu	1372	4	achieved	achieve	VBN
cord-014597-66vd2mdu	1372	5	by	by	IN
cord-014597-66vd2mdu	1372	6	a	a	DT
cord-014597-66vd2mdu	1372	7	combination	combination	NN
cord-014597-66vd2mdu	1372	8	of	of	IN
cord-014597-66vd2mdu	1372	9	expression	expression	NN
cord-014597-66vd2mdu	1372	10	cassette	cassette	NN
cord-014597-66vd2mdu	1372	11	optimization	optimization	NN
cord-014597-66vd2mdu	1372	12	,	,	,
cord-014597-66vd2mdu	1372	13	cell	cell	NN
cord-014597-66vd2mdu	1372	14	engineering	engineering	NN
cord-014597-66vd2mdu	1372	15	and	and	CC
cord-014597-66vd2mdu	1372	16	selection	selection	NN
cord-014597-66vd2mdu	1372	17	process	process	NN
cord-014597-66vd2mdu	1372	18	.	.	.
cord-014597-66vd2mdu	1373	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1373	2	have	have	VBP
cord-014597-66vd2mdu	1373	3	previously	previously	RB
cord-014597-66vd2mdu	1373	4	developed	develop	VBN
cord-014597-66vd2mdu	1373	5	the	the	DT
cord-014597-66vd2mdu	1373	6	cumate	cumate	NN
cord-014597-66vd2mdu	1373	7	gene	gene	NN
cord-014597-66vd2mdu	1373	8	-	-	HYPH
cord-014597-66vd2mdu	1373	9	switch	switch	NN
cord-014597-66vd2mdu	1373	10	,	,	,
cord-014597-66vd2mdu	1373	11	which	which	WDT
cord-014597-66vd2mdu	1373	12	is	be	VBZ
cord-014597-66vd2mdu	1373	13	a	a	DT
cord-014597-66vd2mdu	1373	14	very	very	RB
cord-014597-66vd2mdu	1373	15	efficient	efficient	JJ
cord-014597-66vd2mdu	1373	16	expression	expression	NN
cord-014597-66vd2mdu	1373	17	system	system	NN
cord-014597-66vd2mdu	1373	18	for	for	IN
cord-014597-66vd2mdu	1373	19	protein	protein	NN
cord-014597-66vd2mdu	1373	20	production	production	NN
cord-014597-66vd2mdu	1373	21	[	[	-LRB-
cord-014597-66vd2mdu	1373	22	1	1	CD
cord-014597-66vd2mdu	1373	23	]	]	-RRB-
cord-014597-66vd2mdu	1373	24	.	.	.
cord-014597-66vd2mdu	1374	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1374	2	have	have	VBP
cord-014597-66vd2mdu	1374	3	shown	show	VBN
cord-014597-66vd2mdu	1374	4	that	that	IN
cord-014597-66vd2mdu	1374	5	the	the	DT
cord-014597-66vd2mdu	1374	6	cumate	cumate	NN
cord-014597-66vd2mdu	1374	7	-	-	HYPH
cord-014597-66vd2mdu	1374	8	inducible	inducible	JJ
cord-014597-66vd2mdu	1374	9	promoter	promoter	NN
cord-014597-66vd2mdu	1374	10	(	(	-LRB-
cord-014597-66vd2mdu	1374	11	CR5	CR5	NNP
cord-014597-66vd2mdu	1374	12	)	)	-RRB-
cord-014597-66vd2mdu	1374	13	was	be	VBD
cord-014597-66vd2mdu	1374	14	the	the	DT
cord-014597-66vd2mdu	1374	15	strongest	strong	JJS
cord-014597-66vd2mdu	1374	16	promoter	promoter	NN
cord-014597-66vd2mdu	1374	17	we	-PRON-	PRP
cord-014597-66vd2mdu	1374	18	had	have	VBD
cord-014597-66vd2mdu	1374	19	tested	test	VBN
cord-014597-66vd2mdu	1374	20	so	so	RB
cord-014597-66vd2mdu	1374	21	far	far	RB
cord-014597-66vd2mdu	1374	22	in	in	IN
cord-014597-66vd2mdu	1374	23	Chinese	chinese	JJ
cord-014597-66vd2mdu	1374	24	hamster	hamster	NN
cord-014597-66vd2mdu	1374	25	ovary	ovary	NN
cord-014597-66vd2mdu	1374	26	(	(	-LRB-
cord-014597-66vd2mdu	1374	27	CHO	CHO	NNP
cord-014597-66vd2mdu	1374	28	)	)	-RRB-
cord-014597-66vd2mdu	1374	29	cells	cell	NNS
cord-014597-66vd2mdu	1374	30	.	.	.
cord-014597-66vd2mdu	1375	1	With	with	IN
cord-014597-66vd2mdu	1375	2	this	this	DT
cord-014597-66vd2mdu	1375	3	promoter	promoter	NN
cord-014597-66vd2mdu	1375	4	,	,	,
cord-014597-66vd2mdu	1375	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1375	6	were	be	VBD
cord-014597-66vd2mdu	1375	7	able	able	JJ
cord-014597-66vd2mdu	1375	8	to	to	TO
cord-014597-66vd2mdu	1375	9	generate	generate	VB
cord-014597-66vd2mdu	1375	10	stable	stable	JJ
cord-014597-66vd2mdu	1375	11	CHO	CHO	NNP
cord-014597-66vd2mdu	1375	12	pools	pool	NNS
cord-014597-66vd2mdu	1375	13	capable	capable	JJ
cord-014597-66vd2mdu	1375	14	of	of	IN
cord-014597-66vd2mdu	1375	15	producing	produce	VBG
cord-014597-66vd2mdu	1375	16	high	high	JJ
cord-014597-66vd2mdu	1375	17	levels	level	NNS
cord-014597-66vd2mdu	1375	18	of	of	IN
cord-014597-66vd2mdu	1375	19	a	a	DT
cord-014597-66vd2mdu	1375	20	Fc	Fc	NNP
cord-014597-66vd2mdu	1375	21	fusion	fusion	NN
cord-014597-66vd2mdu	1375	22	protein	protein	NN
cord-014597-66vd2mdu	1375	23	(	(	-LRB-
cord-014597-66vd2mdu	1375	24	900	900	CD
cord-014597-66vd2mdu	1375	25	mg	mg	NNP
cord-014597-66vd2mdu	1375	26	/	/	SYM
cord-014597-66vd2mdu	1375	27	L	L	NNP
cord-014597-66vd2mdu	1375	28	)	)	-RRB-
cord-014597-66vd2mdu	1375	29	,	,	,
cord-014597-66vd2mdu	1375	30	outperforming	outperform	VBG
cord-014597-66vd2mdu	1375	31	by	by	IN
cord-014597-66vd2mdu	1375	32	3	3	CD
cord-014597-66vd2mdu	1375	33	to	to	TO
cord-014597-66vd2mdu	1375	34	4	4	CD
cord-014597-66vd2mdu	1375	35	fold	fold	VB
cord-014597-66vd2mdu	1375	36	those	those	DT
cord-014597-66vd2mdu	1375	37	generated	generate	VBN
cord-014597-66vd2mdu	1375	38	with	with	IN
cord-014597-66vd2mdu	1375	39	CMV5	CMV5	NNP
cord-014597-66vd2mdu	1375	40	and	and	CC
cord-014597-66vd2mdu	1375	41	hybrid	hybrid	JJ
cord-014597-66vd2mdu	1375	42	EF1α	EF1α	NNP
cord-014597-66vd2mdu	1375	43	-	-	HYPH
cord-014597-66vd2mdu	1375	44	HTLV	HTLV	NNP
cord-014597-66vd2mdu	1375	45	constitutive	constitutive	JJ
cord-014597-66vd2mdu	1375	46	promoters	promoter	NNS
cord-014597-66vd2mdu	1375	47	.	.	.
cord-014597-66vd2mdu	1376	1	Besides	besides	IN
cord-014597-66vd2mdu	1376	2	the	the	DT
cord-014597-66vd2mdu	1376	3	strength	strength	NN
cord-014597-66vd2mdu	1376	4	of	of	IN
cord-014597-66vd2mdu	1376	5	the	the	DT
cord-014597-66vd2mdu	1376	6	CR5	CR5	NNP
cord-014597-66vd2mdu	1376	7	promoter	promoter	NN
cord-014597-66vd2mdu	1376	8	,	,	,
cord-014597-66vd2mdu	1376	9	we	-PRON-	PRP
cord-014597-66vd2mdu	1376	10	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	1376	11	that	that	IN
cord-014597-66vd2mdu	1376	12	the	the	DT
cord-014597-66vd2mdu	1376	13	ability	ability	NN
cord-014597-66vd2mdu	1376	14	to	to	TO
cord-014597-66vd2mdu	1376	15	control	control	VB
cord-014597-66vd2mdu	1376	16	both	both	CC
cord-014597-66vd2mdu	1376	17	the	the	DT
cord-014597-66vd2mdu	1376	18	time	time	NN
cord-014597-66vd2mdu	1376	19	and	and	CC
cord-014597-66vd2mdu	1376	20	the	the	DT
cord-014597-66vd2mdu	1376	21	level	level	NN
cord-014597-66vd2mdu	1376	22	of	of	IN
cord-014597-66vd2mdu	1376	23	expression	expression	NN
cord-014597-66vd2mdu	1376	24	during	during	IN
cord-014597-66vd2mdu	1376	25	pool	pool	NN
cord-014597-66vd2mdu	1376	26	generation	generation	NN
cord-014597-66vd2mdu	1376	27	and	and	CC
cord-014597-66vd2mdu	1376	28	maintenance	maintenance	NN
cord-014597-66vd2mdu	1376	29	gave	give	VBD
cord-014597-66vd2mdu	1376	30	a	a	DT
cord-014597-66vd2mdu	1376	31	real	real	JJ
cord-014597-66vd2mdu	1376	32	advantage	advantage	NN
cord-014597-66vd2mdu	1376	33	to	to	IN
cord-014597-66vd2mdu	1376	34	the	the	DT
cord-014597-66vd2mdu	1376	35	inducible	inducible	JJ
cord-014597-66vd2mdu	1376	36	expression	expression	NN
cord-014597-66vd2mdu	1376	37	system	system	NN
cord-014597-66vd2mdu	1376	38	.	.	.
cord-014597-66vd2mdu	1377	1	Indeed	indeed	RB
cord-014597-66vd2mdu	1377	2	,	,	,
cord-014597-66vd2mdu	1377	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1377	4	observed	observe	VBD
cord-014597-66vd2mdu	1377	5	that	that	IN
cord-014597-66vd2mdu	1377	6	keeping	keep	VBG
cord-014597-66vd2mdu	1377	7	the	the	DT
cord-014597-66vd2mdu	1377	8	expression	expression	NN
cord-014597-66vd2mdu	1377	9	OFF	off	RP
cord-014597-66vd2mdu	1377	10	during	during	IN
cord-014597-66vd2mdu	1377	11	selection	selection	NN
cord-014597-66vd2mdu	1377	12	enabled	enable	VBD
cord-014597-66vd2mdu	1377	13	the	the	DT
cord-014597-66vd2mdu	1377	14	generation	generation	NN
cord-014597-66vd2mdu	1377	15	of	of	IN
cord-014597-66vd2mdu	1377	16	pools	pool	NNS
cord-014597-66vd2mdu	1377	17	with	with	IN
cord-014597-66vd2mdu	1377	18	superior	superior	JJ
cord-014597-66vd2mdu	1377	19	productivity	productivity	NN
cord-014597-66vd2mdu	1377	20	compared	compare	VBN
cord-014597-66vd2mdu	1377	21	with	with	IN
cord-014597-66vd2mdu	1377	22	the	the	DT
cord-014597-66vd2mdu	1377	23	pools	pool	NNS
cord-014597-66vd2mdu	1377	24	whose	whose	WP$
cord-014597-66vd2mdu	1377	25	expression	expression	NN
cord-014597-66vd2mdu	1377	26	was	be	VBD
cord-014597-66vd2mdu	1377	27	maintained	maintain	VBN
cord-014597-66vd2mdu	1377	28	ON	on	RP
cord-014597-66vd2mdu	1377	29	.	.	.
cord-014597-66vd2mdu	1378	1	Moreover	moreover	RB
cord-014597-66vd2mdu	1378	2	,	,	,
cord-014597-66vd2mdu	1378	3	preliminary	preliminary	JJ
cord-014597-66vd2mdu	1378	4	results	result	NNS
cord-014597-66vd2mdu	1378	5	suggest	suggest	VBP
cord-014597-66vd2mdu	1378	6	that	that	IN
cord-014597-66vd2mdu	1378	7	keeping	keep	VBG
cord-014597-66vd2mdu	1378	8	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1378	9	protein	protein	NN
cord-014597-66vd2mdu	1378	10	expression	expression	NN
cord-014597-66vd2mdu	1378	11	down	down	RP
cord-014597-66vd2mdu	1378	12	increases	increase	VBZ
cord-014597-66vd2mdu	1378	13	the	the	DT
cord-014597-66vd2mdu	1378	14	frequency	frequency	NN
cord-014597-66vd2mdu	1378	15	of	of	IN
cord-014597-66vd2mdu	1378	16	high	high	JJ
cord-014597-66vd2mdu	1378	17	producer	producer	NN
cord-014597-66vd2mdu	1378	18	clones	clone	NNS
cord-014597-66vd2mdu	1378	19	[	[	-LRB-
cord-014597-66vd2mdu	1378	20	2	2	CD
cord-014597-66vd2mdu	1378	21	]	]	-RRB-
cord-014597-66vd2mdu	1378	22	.	.	.
cord-014597-66vd2mdu	1379	1	Knowing	know	VBG
cord-014597-66vd2mdu	1379	2	that	that	IN
cord-014597-66vd2mdu	1379	3	one	one	CD
cord-014597-66vd2mdu	1379	4	of	of	IN
cord-014597-66vd2mdu	1379	5	the	the	DT
cord-014597-66vd2mdu	1379	6	main	main	JJ
cord-014597-66vd2mdu	1379	7	bottlenecks	bottleneck	NNS
cord-014597-66vd2mdu	1379	8	of	of	IN
cord-014597-66vd2mdu	1379	9	the	the	DT
cord-014597-66vd2mdu	1379	10	successful	successful	JJ
cord-014597-66vd2mdu	1379	11	bioprocessing	bioprocessing	NN
cord-014597-66vd2mdu	1379	12	of	of	IN
cord-014597-66vd2mdu	1379	13	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1379	14	proteins	protein	NNS
cord-014597-66vd2mdu	1379	15	using	use	VBG
cord-014597-66vd2mdu	1379	16	CHO	CHO	NNP
cord-014597-66vd2mdu	1379	17	cells	cell	NNS
cord-014597-66vd2mdu	1379	18	is	be	VBZ
cord-014597-66vd2mdu	1379	19	the	the	DT
cord-014597-66vd2mdu	1379	20	rapid	rapid	JJ
cord-014597-66vd2mdu	1379	21	isolation	isolation	NN
cord-014597-66vd2mdu	1379	22	of	of	IN
cord-014597-66vd2mdu	1379	23	a	a	DT
cord-014597-66vd2mdu	1379	24	high	high	JJ
cord-014597-66vd2mdu	1379	25	producer	producer	NN
cord-014597-66vd2mdu	1379	26	,	,	,
cord-014597-66vd2mdu	1379	27	our	-PRON-	PRP$
cord-014597-66vd2mdu	1379	28	data	datum	NNS
cord-014597-66vd2mdu	1379	29	suggest	suggest	VBP
cord-014597-66vd2mdu	1379	30	that	that	IN
cord-014597-66vd2mdu	1379	31	the	the	DT
cord-014597-66vd2mdu	1379	32	cumate	cumate	NNP
cord-014597-66vd2mdu	1379	33	gene	gene	NN
cord-014597-66vd2mdu	1379	34	-	-	HYPH
cord-014597-66vd2mdu	1379	35	switch	switch	NN
cord-014597-66vd2mdu	1379	36	system	system	NN
cord-014597-66vd2mdu	1379	37	could	could	MD
cord-014597-66vd2mdu	1379	38	be	be	VB
cord-014597-66vd2mdu	1379	39	a	a	DT
cord-014597-66vd2mdu	1379	40	valuable	valuable	JJ
cord-014597-66vd2mdu	1379	41	platform	platform	NN
cord-014597-66vd2mdu	1379	42	for	for	IN
cord-014597-66vd2mdu	1379	43	the	the	DT
cord-014597-66vd2mdu	1379	44	generation	generation	NN
cord-014597-66vd2mdu	1379	45	of	of	IN
cord-014597-66vd2mdu	1379	46	stable	stable	JJ
cord-014597-66vd2mdu	1379	47	clones	clone	NNS
cord-014597-66vd2mdu	1379	48	.	.	.
cord-014597-66vd2mdu	1380	1	The	the	DT
cord-014597-66vd2mdu	1380	2	main	main	JJ
cord-014597-66vd2mdu	1380	3	regulatory	regulatory	JJ
cord-014597-66vd2mdu	1380	4	authorities	authority	NNS
cord-014597-66vd2mdu	1380	5	and	and	CC
cord-014597-66vd2mdu	1380	6	organizations	organization	NNS
cord-014597-66vd2mdu	1380	7	demand	demand	VBP
cord-014597-66vd2mdu	1380	8	proof	proof	NN
cord-014597-66vd2mdu	1380	9	of	of	IN
cord-014597-66vd2mdu	1380	10	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1380	11	for	for	IN
cord-014597-66vd2mdu	1380	12	biotechnological	biotechnological	JJ
cord-014597-66vd2mdu	1380	13	producer	producer	NN
cord-014597-66vd2mdu	1380	14	cells	cell	NNS
cord-014597-66vd2mdu	1380	15	.	.	.
cord-014597-66vd2mdu	1381	1	With	with	IN
cord-014597-66vd2mdu	1381	2	increasing	increase	VBG
cord-014597-66vd2mdu	1381	3	pressure	pressure	NN
cord-014597-66vd2mdu	1381	4	to	to	TO
cord-014597-66vd2mdu	1381	5	shorten	shorten	VB
cord-014597-66vd2mdu	1381	6	timelines	timeline	NNS
cord-014597-66vd2mdu	1381	7	and	and	CC
cord-014597-66vd2mdu	1381	8	to	to	TO
cord-014597-66vd2mdu	1381	9	improve	improve	VB
cord-014597-66vd2mdu	1381	10	drug	drug	NN
cord-014597-66vd2mdu	1381	11	safety	safety	NN
cord-014597-66vd2mdu	1381	12	,	,	,
cord-014597-66vd2mdu	1381	13	technologically	technologically	RB
cord-014597-66vd2mdu	1381	14	advanced	advanced	JJ
cord-014597-66vd2mdu	1381	15	methods	method	NNS
cord-014597-66vd2mdu	1381	16	have	have	VBP
cord-014597-66vd2mdu	1381	17	to	to	TO
cord-014597-66vd2mdu	1381	18	be	be	VB
cord-014597-66vd2mdu	1381	19	established	establish	VBN
cord-014597-66vd2mdu	1381	20	to	to	TO
cord-014597-66vd2mdu	1381	21	ensure	ensure	VB
cord-014597-66vd2mdu	1381	22	that	that	IN
cord-014597-66vd2mdu	1381	23	production	production	NN
cord-014597-66vd2mdu	1381	24	cell	cell	NN
cord-014597-66vd2mdu	1381	25	lines	line	NNS
cord-014597-66vd2mdu	1381	26	are	be	VBP
cord-014597-66vd2mdu	1381	27	derived	derive	VBN
cord-014597-66vd2mdu	1381	28	from	from	IN
cord-014597-66vd2mdu	1381	29	a	a	DT
cord-014597-66vd2mdu	1381	30	single	single	JJ
cord-014597-66vd2mdu	1381	31	progenitor	progenitor	NN
cord-014597-66vd2mdu	1381	32	cell	cell	NN
cord-014597-66vd2mdu	1381	33	.	.	.
cord-014597-66vd2mdu	1382	1	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	1382	2	Stedim	Stedim	NNP
cord-014597-66vd2mdu	1382	3	Cellca	Cellca	NNP
cord-014597-66vd2mdu	1382	4	's	's	POS
cord-014597-66vd2mdu	1382	5	single	single	JJ
cord-014597-66vd2mdu	1382	6	cell	cell	NN
cord-014597-66vd2mdu	1382	7	cloning	cloning	NN
cord-014597-66vd2mdu	1382	8	approach	approach	NN
cord-014597-66vd2mdu	1382	9	is	be	VBZ
cord-014597-66vd2mdu	1382	10	based	base	VBN
cord-014597-66vd2mdu	1382	11	on	on	IN
cord-014597-66vd2mdu	1382	12	one	one	CD
cord-014597-66vd2mdu	1382	13	round	round	NN
cord-014597-66vd2mdu	1382	14	of	of	IN
cord-014597-66vd2mdu	1382	15	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	1382	16	-	-	HYPH
cord-014597-66vd2mdu	1382	17	activated	activate	VBN
cord-014597-66vd2mdu	1382	18	cell	cell	NN
cord-014597-66vd2mdu	1382	19	sorting	sorting	NN
cord-014597-66vd2mdu	1382	20	(	(	-LRB-
cord-014597-66vd2mdu	1382	21	FACS	FACS	NNP
cord-014597-66vd2mdu	1382	22	)	)	-RRB-
cord-014597-66vd2mdu	1382	23	using	use	VBG
cord-014597-66vd2mdu	1382	24	Becton	Becton	NNP
cord-014597-66vd2mdu	1382	25	Dickinson	Dickinson	NNP
cord-014597-66vd2mdu	1382	26	(	(	-LRB-
cord-014597-66vd2mdu	1382	27	BD	BD	NNP
cord-014597-66vd2mdu	1382	28	)	)	-RRB-
cord-014597-66vd2mdu	1383	1	FACSAriaTM	FACSAriaTM	NNP
cord-014597-66vd2mdu	1383	2	Fusion	Fusion	NNP
cord-014597-66vd2mdu	1383	3	cell	cell	NN
cord-014597-66vd2mdu	1383	4	sorter	sorter	NN
cord-014597-66vd2mdu	1383	5	combined	combine	VBN
cord-014597-66vd2mdu	1383	6	with	with	IN
cord-014597-66vd2mdu	1383	7	photodocumentation	photodocumentation	NN
cord-014597-66vd2mdu	1383	8	by	by	IN
cord-014597-66vd2mdu	1383	9	SynenTec	SynenTec	NNP
cord-014597-66vd2mdu	1383	10	Cellavista	Cellavista	NNP
cord-014597-66vd2mdu	1383	11	microscopic	microscopic	JJ
cord-014597-66vd2mdu	1383	12	imaging	imaging	NN
cord-014597-66vd2mdu	1383	13	system	system	NN
cord-014597-66vd2mdu	1383	14	.	.	.
cord-014597-66vd2mdu	1384	1	For	for	IN
cord-014597-66vd2mdu	1384	2	the	the	DT
cord-014597-66vd2mdu	1384	3	approach	approach	NN
cord-014597-66vd2mdu	1384	4	,	,	,
cord-014597-66vd2mdu	1384	5	critical	critical	JJ
cord-014597-66vd2mdu	1384	6	process	process	NN
cord-014597-66vd2mdu	1384	7	parameters	parameter	NNS
cord-014597-66vd2mdu	1384	8	such	such	JJ
cord-014597-66vd2mdu	1384	9	as	as	IN
cord-014597-66vd2mdu	1384	10	different	different	JJ
cord-014597-66vd2mdu	1384	11	cell	cell	NN
cord-014597-66vd2mdu	1384	12	lines	line	NNS
cord-014597-66vd2mdu	1384	13	,	,	,
cord-014597-66vd2mdu	1384	14	viability	viability	NN
cord-014597-66vd2mdu	1384	15	and	and	CC
cord-014597-66vd2mdu	1384	16	cell	cell	NN
cord-014597-66vd2mdu	1384	17	aggregation	aggregation	NN
cord-014597-66vd2mdu	1384	18	levels	level	NNS
cord-014597-66vd2mdu	1384	19	were	be	VBD
cord-014597-66vd2mdu	1384	20	investigated	investigate	VBN
cord-014597-66vd2mdu	1384	21	separately	separately	RB
cord-014597-66vd2mdu	1384	22	to	to	TO
cord-014597-66vd2mdu	1384	23	assess	assess	VB
cord-014597-66vd2mdu	1384	24	their	-PRON-	PRP$
cord-014597-66vd2mdu	1384	25	contribution	contribution	NN
cord-014597-66vd2mdu	1384	26	to	to	IN
cord-014597-66vd2mdu	1384	27	the	the	DT
cord-014597-66vd2mdu	1384	28	probability	probability	NN
cord-014597-66vd2mdu	1384	29	of	of	IN
cord-014597-66vd2mdu	1384	30	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1384	31	.	.	.
cord-014597-66vd2mdu	1385	1	Immediately	immediately	RB
cord-014597-66vd2mdu	1385	2	after	after	IN
cord-014597-66vd2mdu	1385	3	single	single	JJ
cord-014597-66vd2mdu	1385	4	cell	cell	NN
cord-014597-66vd2mdu	1385	5	cloning	clone	VBG
cord-014597-66vd2mdu	1385	6	into	into	IN
cord-014597-66vd2mdu	1385	7	384-well	384-well	CD
cord-014597-66vd2mdu	1385	8	plates	plate	NNS
cord-014597-66vd2mdu	1385	9	(	(	-LRB-
cord-014597-66vd2mdu	1386	1	1	1	LS
cord-014597-66vd2mdu	1386	2	cell/	cell/	NN
cord-014597-66vd2mdu	1386	3	well	well	NN
cord-014597-66vd2mdu	1386	4	)	)	-RRB-
cord-014597-66vd2mdu	1387	1	the	the	DT
cord-014597-66vd2mdu	1387	2	plates	plate	NNS
cord-014597-66vd2mdu	1387	3	were	be	VBD
cord-014597-66vd2mdu	1387	4	centrifuged	centrifuge	VBN
cord-014597-66vd2mdu	1387	5	followed	follow	VBN
cord-014597-66vd2mdu	1387	6	by	by	IN
cord-014597-66vd2mdu	1387	7	imaging	image	VBG
cord-014597-66vd2mdu	1387	8	using	use	VBG
cord-014597-66vd2mdu	1387	9	the	the	DT
cord-014597-66vd2mdu	1387	10	Cellavista	Cellavista	NNP
cord-014597-66vd2mdu	1387	11	(	(	-LRB-
cord-014597-66vd2mdu	1387	12	day	day	NN
cord-014597-66vd2mdu	1387	13	0	0	CD
cord-014597-66vd2mdu	1387	14	)	)	-RRB-
cord-014597-66vd2mdu	1387	15	.	.	.
cord-014597-66vd2mdu	1388	1	Further	further	JJ
cord-014597-66vd2mdu	1388	2	Cellavista	cellavista	JJ
cord-014597-66vd2mdu	1388	3	images	image	NNS
cord-014597-66vd2mdu	1388	4	are	be	VBP
cord-014597-66vd2mdu	1388	5	taken	take	VBN
cord-014597-66vd2mdu	1388	6	on	on	IN
cord-014597-66vd2mdu	1388	7	day	day	NN
cord-014597-66vd2mdu	1388	8	1	1	CD
cord-014597-66vd2mdu	1388	9	,	,	,
cord-014597-66vd2mdu	1388	10	day	day	NN
cord-014597-66vd2mdu	1388	11	2	2	CD
cord-014597-66vd2mdu	1388	12	and	and	CC
cord-014597-66vd2mdu	1388	13	on	on	IN
cord-014597-66vd2mdu	1388	14	one	one	CD
cord-014597-66vd2mdu	1388	15	day	day	NN
cord-014597-66vd2mdu	1388	16	between	between	IN
cord-014597-66vd2mdu	1388	17	day	day	NN
cord-014597-66vd2mdu	1388	18	5	5	CD
cord-014597-66vd2mdu	1388	19	and	and	CC
cord-014597-66vd2mdu	1388	20	7	7	CD
cord-014597-66vd2mdu	1388	21	.	.	.
cord-014597-66vd2mdu	1389	1	Outgrowth	outgrowth	NN
cord-014597-66vd2mdu	1389	2	was	be	VBD
cord-014597-66vd2mdu	1389	3	defined	define	VBN
cord-014597-66vd2mdu	1389	4	at	at	IN
cord-014597-66vd2mdu	1389	5	day	day	NN
cord-014597-66vd2mdu	1389	6	14	14	CD
cord-014597-66vd2mdu	1389	7	.	.	.
cord-014597-66vd2mdu	1390	1	8	8	CD
cord-014597-66vd2mdu	1390	2	cell	cell	NN
cord-014597-66vd2mdu	1390	3	lines	line	NNS
cord-014597-66vd2mdu	1390	4	expressing	express	VBG
cord-014597-66vd2mdu	1390	5	different	different	JJ
cord-014597-66vd2mdu	1390	6	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1390	7	products	product	NNS
cord-014597-66vd2mdu	1390	8	were	be	VBD
cord-014597-66vd2mdu	1390	9	investigated	investigate	VBN
cord-014597-66vd2mdu	1390	10	to	to	TO
cord-014597-66vd2mdu	1390	11	calculate	calculate	VB
cord-014597-66vd2mdu	1390	12	probability	probability	NN
cord-014597-66vd2mdu	1390	13	of	of	IN
cord-014597-66vd2mdu	1390	14	having	have	VBG
cord-014597-66vd2mdu	1390	15	≥	≥	CD
cord-014597-66vd2mdu	1390	16	2	2	CD
cord-014597-66vd2mdu	1390	17	cells	cell	NNS
cord-014597-66vd2mdu	1390	18	/	/	SYM
cord-014597-66vd2mdu	1390	19	well	well	RB
cord-014597-66vd2mdu	1390	20	after	after	IN
cord-014597-66vd2mdu	1390	21	FACS	FACS	NNP
cord-014597-66vd2mdu	1390	22	sorting	sort	VBG
cord-014597-66vd2mdu	1390	23	P(d	P(d	NNP
cord-014597-66vd2mdu	1390	24	)	)	-RRB-
cord-014597-66vd2mdu	1390	25	,	,	,
cord-014597-66vd2mdu	1390	26	the	the	DT
cord-014597-66vd2mdu	1390	27	apparent	apparent	JJ
cord-014597-66vd2mdu	1390	28	probability	probability	NN
cord-014597-66vd2mdu	1390	29	P(i	P(i	NNP
cord-014597-66vd2mdu	1390	30	)	)	-RRB-
cord-014597-66vd2mdu	1390	31	of	of	IN
cord-014597-66vd2mdu	1390	32	having	have	VBG
cord-014597-66vd2mdu	1390	33	ghostcells	ghostcell	NNS
cord-014597-66vd2mdu	1390	34	(	(	-LRB-
cord-014597-66vd2mdu	1390	35	cells	cell	NNS
cord-014597-66vd2mdu	1390	36	that	that	WDT
cord-014597-66vd2mdu	1390	37	are	be	VBP
cord-014597-66vd2mdu	1390	38	out	out	RB
cord-014597-66vd2mdu	1390	39	-	-	HYPH
cord-014597-66vd2mdu	1390	40	of	of	IN
cord-014597-66vd2mdu	1390	41	-	-	HYPH
cord-014597-66vd2mdu	1390	42	focus	focus	NN
cord-014597-66vd2mdu	1390	43	and	and	CC
cord-014597-66vd2mdu	1390	44	,	,	,
cord-014597-66vd2mdu	1390	45	thus	thus	RB
cord-014597-66vd2mdu	1390	46	,	,	,
cord-014597-66vd2mdu	1390	47	are	be	VBP
cord-014597-66vd2mdu	1390	48	not	not	RB
cord-014597-66vd2mdu	1390	49	visible	visible	JJ
cord-014597-66vd2mdu	1390	50	during	during	IN
cord-014597-66vd2mdu	1390	51	initial	initial	JJ
cord-014597-66vd2mdu	1390	52	microscopic	microscopic	JJ
cord-014597-66vd2mdu	1390	53	imaging	imaging	NN
cord-014597-66vd2mdu	1390	54	)	)	-RRB-
cord-014597-66vd2mdu	1390	55	,	,	,
cord-014597-66vd2mdu	1390	56	and	and	CC
cord-014597-66vd2mdu	1390	57	the	the	DT
cord-014597-66vd2mdu	1390	58	apparent	apparent	JJ
cord-014597-66vd2mdu	1390	59	probability	probability	NN
cord-014597-66vd2mdu	1390	60	P(k	P(k	NNP
cord-014597-66vd2mdu	1390	61	)	)	-RRB-
cord-014597-66vd2mdu	1390	62	of	of	IN
cord-014597-66vd2mdu	1390	63	having	have	VBG
cord-014597-66vd2mdu	1390	64	ghostcells	ghostcell	NNS
cord-014597-66vd2mdu	1390	65	that	that	WDT
cord-014597-66vd2mdu	1390	66	outgrow	outgrow	VBP
cord-014597-66vd2mdu	1390	67	the	the	DT
cord-014597-66vd2mdu	1390	68	384-well	384-well	CD
cord-014597-66vd2mdu	1390	69	stage	stage	NN
cord-014597-66vd2mdu	1391	1	(	(	-LRB-
cord-014597-66vd2mdu	1391	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1391	3	.	.	NNP
cord-014597-66vd2mdu	1391	4	1	1	CD
cord-014597-66vd2mdu	1391	5	)	)	-RRB-
cord-014597-66vd2mdu	1391	6	.	.	.
cord-014597-66vd2mdu	1392	1	Using	use	VBG
cord-014597-66vd2mdu	1392	2	these	these	DT
cord-014597-66vd2mdu	1392	3	results	result	NNS
cord-014597-66vd2mdu	1392	4	,	,	,
cord-014597-66vd2mdu	1392	5	the	the	DT
cord-014597-66vd2mdu	1392	6	probability	probability	NN
cord-014597-66vd2mdu	1392	7	of	of	IN
cord-014597-66vd2mdu	1392	8	obtaining	obtain	VBG
cord-014597-66vd2mdu	1392	9	a	a	DT
cord-014597-66vd2mdu	1392	10	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1392	11	cell	cell	NN
cord-014597-66vd2mdu	1392	12	by	by	IN
cord-014597-66vd2mdu	1392	13	using	use	VBG
cord-014597-66vd2mdu	1392	14	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	1392	15	Stedim	Stedim	NNP
cord-014597-66vd2mdu	1392	16	Cellca	Cellca	NNP
cord-014597-66vd2mdu	1392	17	's	's	POS
cord-014597-66vd2mdu	1392	18	single	single	JJ
cord-014597-66vd2mdu	1392	19	cell	cell	NN
cord-014597-66vd2mdu	1392	20	cloning	cloning	NN
cord-014597-66vd2mdu	1392	21	approach	approach	NN
cord-014597-66vd2mdu	1392	22	was	be	VBD
cord-014597-66vd2mdu	1392	23	determined	determine	VBN
cord-014597-66vd2mdu	1392	24	(	(	-LRB-
cord-014597-66vd2mdu	1392	25	Table	table	NN
cord-014597-66vd2mdu	1392	26	1	1	CD
cord-014597-66vd2mdu	1392	27	)	)	-RRB-
cord-014597-66vd2mdu	1392	28	by	by	IN
cord-014597-66vd2mdu	1392	29	Conservative	conservative	JJ
cord-014597-66vd2mdu	1392	30	examination	examination	NN
cord-014597-66vd2mdu	1392	31	:	:	:
cord-014597-66vd2mdu	1392	32	P(monoclonal	p(monoclonal	JJ
cord-014597-66vd2mdu	1392	33	,	,	,
cord-014597-66vd2mdu	1392	34	conservative	conservative	JJ
cord-014597-66vd2mdu	1392	35	)	)	-RRB-
cord-014597-66vd2mdu	1392	36	=	=	NFP
cord-014597-66vd2mdu	1393	1	1	1	LS
cord-014597-66vd2mdu	1393	2	-(P(d	-(p(d	ADD
cord-014597-66vd2mdu	1393	3	)	)	-RRB-
cord-014597-66vd2mdu	1394	1	x	x	NNP
cord-014597-66vd2mdu	1394	2	P(i	P(i	NNP
cord-014597-66vd2mdu	1394	3	)	)	-RRB-
cord-014597-66vd2mdu	1394	4	)	)	-RRB-
cord-014597-66vd2mdu	1395	1	Realistic	realistic	JJ
cord-014597-66vd2mdu	1395	2	examination	examination	NN
cord-014597-66vd2mdu	1395	3	:	:	:
cord-014597-66vd2mdu	1395	4	P(monoclonal	p(monoclonal	JJ
cord-014597-66vd2mdu	1395	5	,	,	,
cord-014597-66vd2mdu	1395	6	realistic	realistic	JJ
cord-014597-66vd2mdu	1395	7	)	)	-RRB-
cord-014597-66vd2mdu	1395	8	=	=	NFP
cord-014597-66vd2mdu	1396	1	1	1	LS
cord-014597-66vd2mdu	1396	2	-(P(d	-(p(d	ADD
cord-014597-66vd2mdu	1396	3	)	)	-RRB-
cord-014597-66vd2mdu	1397	1	x	x	NNP
cord-014597-66vd2mdu	1397	2	P(k	P(k	NNP
cord-014597-66vd2mdu	1397	3	)	)	-RRB-
cord-014597-66vd2mdu	1397	4	)	)	-RRB-
cord-014597-66vd2mdu	1398	1	Cell	cell	NN
cord-014597-66vd2mdu	1398	2	pools	pool	NNS
cord-014597-66vd2mdu	1398	3	with	with	IN
cord-014597-66vd2mdu	1398	4	low	low	JJ
cord-014597-66vd2mdu	1398	5	viability	viability	NN
cord-014597-66vd2mdu	1398	6	can	can	MD
cord-014597-66vd2mdu	1398	7	theoretically	theoretically	RB
cord-014597-66vd2mdu	1398	8	impact	impact	VB
cord-014597-66vd2mdu	1398	9	the	the	DT
cord-014597-66vd2mdu	1398	10	probability	probability	NN
cord-014597-66vd2mdu	1398	11	of	of	IN
cord-014597-66vd2mdu	1398	12	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1398	13	by	by	IN
cord-014597-66vd2mdu	1398	14	e.g.	e.g.	RB
cord-014597-66vd2mdu	1398	15	diminishing	diminish	VBG
cord-014597-66vd2mdu	1398	16	microscopic	microscopic	JJ
cord-014597-66vd2mdu	1398	17	imaging	imaging	NN
cord-014597-66vd2mdu	1398	18	quality	quality	NN
cord-014597-66vd2mdu	1398	19	(	(	-LRB-
cord-014597-66vd2mdu	1398	20	cell	cell	NN
cord-014597-66vd2mdu	1398	21	debris	debris	NN
cord-014597-66vd2mdu	1398	22	)	)	-RRB-
cord-014597-66vd2mdu	1398	23	.	.	.
cord-014597-66vd2mdu	1399	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1399	2	,	,	,
cord-014597-66vd2mdu	1399	3	pool	pool	NN
cord-014597-66vd2mdu	1399	4	cell	cell	NN
cord-014597-66vd2mdu	1399	5	line	line	NN
cord-014597-66vd2mdu	1399	6	1	1	CD
cord-014597-66vd2mdu	1399	7	with	with	IN
cord-014597-66vd2mdu	1399	8	very	very	RB
cord-014597-66vd2mdu	1399	9	low	low	JJ
cord-014597-66vd2mdu	1399	10	viability	viability	NN
cord-014597-66vd2mdu	1399	11	(	(	-LRB-
cord-014597-66vd2mdu	1399	12	≥36	≥36	VBZ
cord-014597-66vd2mdu	1399	13	%	%	NN
cord-014597-66vd2mdu	1399	14	)	)	-RRB-
cord-014597-66vd2mdu	1399	15	was	be	VBD
cord-014597-66vd2mdu	1399	16	used	use	VBN
cord-014597-66vd2mdu	1399	17	to	to	TO
cord-014597-66vd2mdu	1399	18	demonstrate	demonstrate	VB
cord-014597-66vd2mdu	1399	19	,	,	,
cord-014597-66vd2mdu	1399	20	that	that	IN
cord-014597-66vd2mdu	1399	21	the	the	DT
cord-014597-66vd2mdu	1399	22	probability	probability	NN
cord-014597-66vd2mdu	1399	23	of	of	IN
cord-014597-66vd2mdu	1399	24	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1399	25	is	be	VBZ
cord-014597-66vd2mdu	1399	26	still	still	RB
cord-014597-66vd2mdu	1399	27	99.9	99.9	CD
cord-014597-66vd2mdu	1399	28	%	%	NN
cord-014597-66vd2mdu	1399	29	in	in	IN
cord-014597-66vd2mdu	1399	30	case	case	NN
cord-014597-66vd2mdu	1399	31	of	of	IN
cord-014597-66vd2mdu	1399	32	low	low	JJ
cord-014597-66vd2mdu	1399	33	viability	viability	NN
cord-014597-66vd2mdu	1399	34	on	on	IN
cord-014597-66vd2mdu	1399	35	day	day	NN
cord-014597-66vd2mdu	1399	36	of	of	IN
cord-014597-66vd2mdu	1399	37	sorting	sorting	NN
cord-014597-66vd2mdu	1399	38	:	:	:
cord-014597-66vd2mdu	1399	39	P(monoclonal	p(monoclonal	JJ
cord-014597-66vd2mdu	1399	40	,	,	,
cord-014597-66vd2mdu	1399	41	conservative	conservative	JJ
cord-014597-66vd2mdu	1399	42	)	)	-RRB-
cord-014597-66vd2mdu	1399	43	=	=	NFP
cord-014597-66vd2mdu	1400	1	P(d	p(d	JJ
cord-014597-66vd2mdu	1400	2	)	)	-RRB-
cord-014597-66vd2mdu	1401	1	x	x	NNP
cord-014597-66vd2mdu	1401	2	P(i	P(i	NNP
cord-014597-66vd2mdu	1401	3	)	)	-RRB-
cord-014597-66vd2mdu	1401	4	=	=	NFP
cord-014597-66vd2mdu	1402	1	99.9	99.9	CD
cord-014597-66vd2mdu	1402	2	%	%	NN
cord-014597-66vd2mdu	1402	3	P(monoclonal	p(monoclonal	JJ
cord-014597-66vd2mdu	1402	4	,	,	,
cord-014597-66vd2mdu	1402	5	realistic	realistic	JJ
cord-014597-66vd2mdu	1402	6	)	)	-RRB-
cord-014597-66vd2mdu	1403	1	=	=	SYM
cord-014597-66vd2mdu	1403	2	P(d	p(d	ADD
cord-014597-66vd2mdu	1403	3	)	)	-RRB-
cord-014597-66vd2mdu	1404	1	x	x	NNP
cord-014597-66vd2mdu	1404	2	P(k	P(k	NNP
cord-014597-66vd2mdu	1404	3	)	)	-RRB-
cord-014597-66vd2mdu	1404	4	=	=	NFP
cord-014597-66vd2mdu	1405	1	99.9	99.9	CD
cord-014597-66vd2mdu	1405	2	%	%	NN
cord-014597-66vd2mdu	1406	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1406	2	,	,	,
cord-014597-66vd2mdu	1406	3	cell	cell	NN
cord-014597-66vd2mdu	1406	4	pools	pool	NNS
cord-014597-66vd2mdu	1406	5	with	with	IN
cord-014597-66vd2mdu	1406	6	high	high	JJ
cord-014597-66vd2mdu	1406	7	aggregation	aggregation	NN
cord-014597-66vd2mdu	1406	8	levels	level	NNS
cord-014597-66vd2mdu	1406	9	can	can	MD
cord-014597-66vd2mdu	1406	10	theoretically	theoretically	RB
cord-014597-66vd2mdu	1406	11	impact	impact	VB
cord-014597-66vd2mdu	1406	12	the	the	DT
cord-014597-66vd2mdu	1406	13	probability	probability	NN
cord-014597-66vd2mdu	1406	14	of	of	IN
cord-014597-66vd2mdu	1406	15	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1406	16	by	by	IN
cord-014597-66vd2mdu	1406	17	sticking	stick	VBG
cord-014597-66vd2mdu	1406	18	together	together	RB
cord-014597-66vd2mdu	1406	19	during	during	IN
cord-014597-66vd2mdu	1406	20	FACS	FACS	NNP
cord-014597-66vd2mdu	1406	21	sorting	sort	VBG
cord-014597-66vd2mdu	1406	22	and	and	CC
cord-014597-66vd2mdu	1406	23	therefore	therefore	RB
cord-014597-66vd2mdu	1406	24	increase	increase	VB
cord-014597-66vd2mdu	1406	25	the	the	DT
cord-014597-66vd2mdu	1406	26	probability	probability	NN
cord-014597-66vd2mdu	1406	27	P(d	p(d	JJ
cord-014597-66vd2mdu	1406	28	)	)	-RRB-
cord-014597-66vd2mdu	1406	29	of	of	IN
cord-014597-66vd2mdu	1406	30	having	have	VBG
cord-014597-66vd2mdu	1406	31	≥	≥	CD
cord-014597-66vd2mdu	1406	32	2	2	CD
cord-014597-66vd2mdu	1406	33	cells	cell	NNS
cord-014597-66vd2mdu	1406	34	/	/	SYM
cord-014597-66vd2mdu	1406	35	droplet	droplet	NN
cord-014597-66vd2mdu	1406	36	.	.	.
cord-014597-66vd2mdu	1407	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1407	2	,	,	,
cord-014597-66vd2mdu	1407	3	pool	pool	NN
cord-014597-66vd2mdu	1407	4	cell	cell	NN
cord-014597-66vd2mdu	1407	5	line	line	NN
cord-014597-66vd2mdu	1407	6	8	8	CD
cord-014597-66vd2mdu	1407	7	with	with	IN
cord-014597-66vd2mdu	1407	8	high	high	JJ
cord-014597-66vd2mdu	1407	9	aggregation	aggregation	NN
cord-014597-66vd2mdu	1407	10	levels	level	NNS
cord-014597-66vd2mdu	1407	11	(	(	-LRB-
cord-014597-66vd2mdu	1407	12	≥11.1	≥11.1	JJR
cord-014597-66vd2mdu	1407	13	%	%	NN
cord-014597-66vd2mdu	1407	14	)	)	-RRB-
cord-014597-66vd2mdu	1407	15	was	be	VBD
cord-014597-66vd2mdu	1407	16	used	use	VBN
cord-014597-66vd2mdu	1407	17	to	to	TO
cord-014597-66vd2mdu	1407	18	demonstrate	demonstrate	VB
cord-014597-66vd2mdu	1407	19	,	,	,
cord-014597-66vd2mdu	1407	20	that	that	IN
cord-014597-66vd2mdu	1407	21	the	the	DT
cord-014597-66vd2mdu	1407	22	probability	probability	NN
cord-014597-66vd2mdu	1407	23	of	of	IN
cord-014597-66vd2mdu	1407	24	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1407	25	is	be	VBZ
cord-014597-66vd2mdu	1407	26	still	still	RB
cord-014597-66vd2mdu	1407	27	≥	≥	CD
cord-014597-66vd2mdu	1407	28	99.9	99.9	CD
cord-014597-66vd2mdu	1407	29	%	%	NN
cord-014597-66vd2mdu	1407	30	in	in	IN
cord-014597-66vd2mdu	1407	31	case	case	NN
cord-014597-66vd2mdu	1407	32	of	of	IN
cord-014597-66vd2mdu	1407	33	highly	highly	RB
cord-014597-66vd2mdu	1407	34	aggregated	aggregated	JJ
cord-014597-66vd2mdu	1407	35	cell	cell	NN
cord-014597-66vd2mdu	1407	36	pools	pool	NNS
cord-014597-66vd2mdu	1407	37	on	on	IN
cord-014597-66vd2mdu	1407	38	day	day	NN
cord-014597-66vd2mdu	1407	39	of	of	IN
cord-014597-66vd2mdu	1407	40	sorting	sorting	NN
cord-014597-66vd2mdu	1407	41	:	:	:
cord-014597-66vd2mdu	1407	42	P(monoclonal	p(monoclonal	JJ
cord-014597-66vd2mdu	1407	43	,	,	,
cord-014597-66vd2mdu	1407	44	conservative	conservative	JJ
cord-014597-66vd2mdu	1407	45	)	)	-RRB-
cord-014597-66vd2mdu	1407	46	=	=	NFP
cord-014597-66vd2mdu	1408	1	P(d	p(d	JJ
cord-014597-66vd2mdu	1408	2	)	)	-RRB-
cord-014597-66vd2mdu	1409	1	x	x	NNP
cord-014597-66vd2mdu	1409	2	P(i	P(i	NNP
cord-014597-66vd2mdu	1409	3	)	)	-RRB-
cord-014597-66vd2mdu	1409	4	=	=	NFP
cord-014597-66vd2mdu	1410	1	>	>	XX
cord-014597-66vd2mdu	1411	1	99.9	99.9	CD
cord-014597-66vd2mdu	1411	2	%	%	NN
cord-014597-66vd2mdu	1411	3	P(monoclonal	p(monoclonal	JJ
cord-014597-66vd2mdu	1411	4	,	,	,
cord-014597-66vd2mdu	1411	5	realistic	realistic	JJ
cord-014597-66vd2mdu	1411	6	)	)	-RRB-
cord-014597-66vd2mdu	1412	1	=	=	SYM
cord-014597-66vd2mdu	1412	2	P(d	p(d	ADD
cord-014597-66vd2mdu	1412	3	)	)	-RRB-
cord-014597-66vd2mdu	1413	1	x	x	NNP
cord-014597-66vd2mdu	1413	2	P(k	P(k	NNP
cord-014597-66vd2mdu	1413	3	)	)	-RRB-
cord-014597-66vd2mdu	1413	4	=	=	NFP
cord-014597-66vd2mdu	1414	1	>	>	XX
cord-014597-66vd2mdu	1415	1	99.9	99.9	CD
cord-014597-66vd2mdu	1415	2	%	%	NN
cord-014597-66vd2mdu	1415	3	Conclusions	conclusion	NNS
cord-014597-66vd2mdu	1416	1	In	in	IN
cord-014597-66vd2mdu	1416	2	summary	summary	NN
cord-014597-66vd2mdu	1416	3	,	,	,
cord-014597-66vd2mdu	1416	4	there	there	EX
cord-014597-66vd2mdu	1416	5	is	be	VBZ
cord-014597-66vd2mdu	1416	6	no	no	DT
cord-014597-66vd2mdu	1416	7	obvious	obvious	JJ
cord-014597-66vd2mdu	1416	8	correlation	correlation	NN
cord-014597-66vd2mdu	1416	9	between	between	IN
cord-014597-66vd2mdu	1416	10	protein	protein	NN
cord-014597-66vd2mdu	1416	11	product	product	NN
cord-014597-66vd2mdu	1416	12	type	type	NN
cord-014597-66vd2mdu	1416	13	and	and	CC
cord-014597-66vd2mdu	1416	14	the	the	DT
cord-014597-66vd2mdu	1416	15	determined	determined	JJ
cord-014597-66vd2mdu	1416	16	probabilities	probability	NNS
cord-014597-66vd2mdu	1416	17	for	for	IN
cord-014597-66vd2mdu	1416	18	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1416	19	.	.	.
cord-014597-66vd2mdu	1417	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1417	2	,	,	,
cord-014597-66vd2mdu	1417	3	pools	pool	NNS
cord-014597-66vd2mdu	1417	4	with	with	IN
cord-014597-66vd2mdu	1417	5	a	a	DT
cord-014597-66vd2mdu	1417	6	viability	viability	NN
cord-014597-66vd2mdu	1417	7	as	as	RB
cord-014597-66vd2mdu	1417	8	low	low	JJ
cord-014597-66vd2mdu	1417	9	as	as	IN
cord-014597-66vd2mdu	1417	10	36	36	CD
cord-014597-66vd2mdu	1417	11	%	%	NN
cord-014597-66vd2mdu	1417	12	and	and	CC
cord-014597-66vd2mdu	1417	13	pools	pool	NNS
cord-014597-66vd2mdu	1417	14	with	with	IN
cord-014597-66vd2mdu	1417	15	an	an	DT
cord-014597-66vd2mdu	1417	16	aggregation	aggregation	NN
cord-014597-66vd2mdu	1417	17	level	level	NN
cord-014597-66vd2mdu	1417	18	as	as	RB
cord-014597-66vd2mdu	1417	19	high	high	JJ
cord-014597-66vd2mdu	1417	20	as	as	IN
cord-014597-66vd2mdu	1417	21	11.1	11.1	CD
cord-014597-66vd2mdu	1417	22	%	%	NN
cord-014597-66vd2mdu	1417	23	can	can	MD
cord-014597-66vd2mdu	1417	24	be	be	VB
cord-014597-66vd2mdu	1417	25	used	use	VBN
cord-014597-66vd2mdu	1417	26	for	for	IN
cord-014597-66vd2mdu	1417	27	SCC	SCC	NNP
cord-014597-66vd2mdu	1417	28	resulting	result	VBG
cord-014597-66vd2mdu	1417	29	in	in	IN
cord-014597-66vd2mdu	1417	30	acceptable	acceptable	JJ
cord-014597-66vd2mdu	1417	31	probabilities	probability	NNS
cord-014597-66vd2mdu	1417	32	of	of	IN
cord-014597-66vd2mdu	1417	33	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1417	34	.	.	.
cord-014597-66vd2mdu	1418	1	Background	Background	NNP
cord-014597-66vd2mdu	1418	2	ICH	ICH	NNP
cord-014597-66vd2mdu	1418	3	guidance	guidance	NN
cord-014597-66vd2mdu	1418	4	[	[	-LRB-
cord-014597-66vd2mdu	1418	5	1	1	CD
cord-014597-66vd2mdu	1418	6	]	]	-RRB-
cord-014597-66vd2mdu	1418	7	requires	require	VBZ
cord-014597-66vd2mdu	1418	8	that	that	IN
cord-014597-66vd2mdu	1418	9	any	any	DT
cord-014597-66vd2mdu	1418	10	cell	cell	NN
cord-014597-66vd2mdu	1418	11	line	line	NN
cord-014597-66vd2mdu	1418	12	used	use	VBN
cord-014597-66vd2mdu	1418	13	to	to	TO
cord-014597-66vd2mdu	1418	14	produce	produce	VB
cord-014597-66vd2mdu	1418	15	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	1418	16	originates	originate	VBZ
cord-014597-66vd2mdu	1418	17	from	from	IN
cord-014597-66vd2mdu	1418	18	a	a	DT
cord-014597-66vd2mdu	1418	19	single	single	JJ
cord-014597-66vd2mdu	1418	20	progenitor	progenitor	NN
cord-014597-66vd2mdu	1418	21	cell	cell	NN
cord-014597-66vd2mdu	1418	22	.	.	.
cord-014597-66vd2mdu	1419	1	Recently	recently	RB
cord-014597-66vd2mdu	1419	2	,	,	,
cord-014597-66vd2mdu	1419	3	there	there	EX
cord-014597-66vd2mdu	1419	4	has	have	VBZ
cord-014597-66vd2mdu	1419	5	been	be	VBN
cord-014597-66vd2mdu	1419	6	increased	increase	VBN
cord-014597-66vd2mdu	1419	7	scrutiny	scrutiny	NN
cord-014597-66vd2mdu	1419	8	of	of	IN
cord-014597-66vd2mdu	1419	9	the	the	DT
cord-014597-66vd2mdu	1419	10	method(s	method(s	NNP
cord-014597-66vd2mdu	1419	11	)	)	-RRB-
cord-014597-66vd2mdu	1419	12	used	use	VBD
cord-014597-66vd2mdu	1419	13	to	to	TO
cord-014597-66vd2mdu	1419	14	achieve	achieve	VB
cord-014597-66vd2mdu	1419	15	this	this	DT
cord-014597-66vd2mdu	1419	16	requirement	requirement	NN
cord-014597-66vd2mdu	1419	17	.	.	.
cord-014597-66vd2mdu	1420	1	Here	here	RB
cord-014597-66vd2mdu	1420	2	,	,	,
cord-014597-66vd2mdu	1420	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1420	4	review	review	VBP
cord-014597-66vd2mdu	1420	5	the	the	DT
cord-014597-66vd2mdu	1420	6	suitability	suitability	NN
cord-014597-66vd2mdu	1420	7	of	of	IN
cord-014597-66vd2mdu	1420	8	the	the	DT
cord-014597-66vd2mdu	1420	9	legacy	legacy	NN
cord-014597-66vd2mdu	1420	10	capillary	capillary	NN
cord-014597-66vd2mdu	1420	11	aided	aid	VBD
cord-014597-66vd2mdu	1420	12	cell	cell	NN
cord-014597-66vd2mdu	1420	13	cloning	cloning	NN
cord-014597-66vd2mdu	1420	14	(	(	-LRB-
cord-014597-66vd2mdu	1420	15	CACC	CACC	NNP
cord-014597-66vd2mdu	1420	16	)	)	-RRB-
cord-014597-66vd2mdu	1420	17	method	method	NN
cord-014597-66vd2mdu	1420	18	in	in	IN
cord-014597-66vd2mdu	1420	19	light	light	NN
cord-014597-66vd2mdu	1420	20	of	of	IN
cord-014597-66vd2mdu	1420	21	this	this	DT
cord-014597-66vd2mdu	1420	22	changing	change	VBG
cord-014597-66vd2mdu	1420	23	landscape	landscape	NN
cord-014597-66vd2mdu	1420	24	of	of	IN
cord-014597-66vd2mdu	1420	25	expectations	expectation	NNS
cord-014597-66vd2mdu	1420	26	.	.	.
cord-014597-66vd2mdu	1421	1	The	the	DT
cord-014597-66vd2mdu	1421	2	CACC	CACC	NNP
cord-014597-66vd2mdu	1421	3	method	method	NN
cord-014597-66vd2mdu	1421	4	is	be	VBZ
cord-014597-66vd2mdu	1421	5	based	base	VBN
cord-014597-66vd2mdu	1421	6	on	on	IN
cord-014597-66vd2mdu	1421	7	the	the	DT
cord-014597-66vd2mdu	1421	8	'	'	``
cord-014597-66vd2mdu	1421	9	spotting	spot	VBG
cord-014597-66vd2mdu	1421	10	'	'	''
cord-014597-66vd2mdu	1421	11	technique	technique	NN
cord-014597-66vd2mdu	1421	12	[	[	-LRB-
cord-014597-66vd2mdu	1421	13	2	2	CD
cord-014597-66vd2mdu	1421	14	]	]	-RRB-
cord-014597-66vd2mdu	1421	15	and	and	CC
cord-014597-66vd2mdu	1421	16	relies	rely	VBZ
cord-014597-66vd2mdu	1421	17	on	on	IN
cord-014597-66vd2mdu	1421	18	independent	independent	JJ
cord-014597-66vd2mdu	1421	19	visual	visual	JJ
cord-014597-66vd2mdu	1421	20	conformation	conformation	NN
cord-014597-66vd2mdu	1421	21	by	by	IN
cord-014597-66vd2mdu	1421	22	two	two	CD
cord-014597-66vd2mdu	1421	23	scientists	scientist	NNS
cord-014597-66vd2mdu	1421	24	of	of	IN
cord-014597-66vd2mdu	1421	25	the	the	DT
cord-014597-66vd2mdu	1421	26	presence	presence	NN
cord-014597-66vd2mdu	1421	27	of	of	IN
cord-014597-66vd2mdu	1421	28	a	a	DT
cord-014597-66vd2mdu	1421	29	single	single	JJ
cord-014597-66vd2mdu	1421	30	cell	cell	NN
cord-014597-66vd2mdu	1421	31	in	in	IN
cord-014597-66vd2mdu	1421	32	a	a	DT
cord-014597-66vd2mdu	1421	33	1	1	CD
cord-014597-66vd2mdu	1421	34	μL	μl	NN
cord-014597-66vd2mdu	1421	35	droplet	droplet	NN
cord-014597-66vd2mdu	1421	36	.	.	.
cord-014597-66vd2mdu	1422	1	This	this	DT
cord-014597-66vd2mdu	1422	2	method	method	NN
cord-014597-66vd2mdu	1422	3	achieves	achieve	VBZ
cord-014597-66vd2mdu	1422	4	a	a	DT
cord-014597-66vd2mdu	1422	5	high	high	JJ
cord-014597-66vd2mdu	1422	6	probability	probability	NN
cord-014597-66vd2mdu	1422	7	of	of	IN
cord-014597-66vd2mdu	1422	8	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1422	9	in	in	IN
cord-014597-66vd2mdu	1422	10	one	one	CD
cord-014597-66vd2mdu	1422	11	cloning	clone	VBG
cord-014597-66vd2mdu	1422	12	round	round	RB
cord-014597-66vd2mdu	1422	13	.	.	.
cord-014597-66vd2mdu	1423	1	Although	although	IN
cord-014597-66vd2mdu	1423	2	the	the	DT
cord-014597-66vd2mdu	1423	3	method	method	NN
cord-014597-66vd2mdu	1423	4	has	have	VBZ
cord-014597-66vd2mdu	1423	5	since	since	IN
cord-014597-66vd2mdu	1423	6	been	be	VBN
cord-014597-66vd2mdu	1423	7	replaced	replace	VBN
cord-014597-66vd2mdu	1423	8	by	by	IN
cord-014597-66vd2mdu	1423	9	FACS	FACS	NNP
cord-014597-66vd2mdu	1423	10	single	single	JJ
cord-014597-66vd2mdu	1423	11	cell	cell	NN
cord-014597-66vd2mdu	1423	12	deposition	deposition	NN
cord-014597-66vd2mdu	1423	13	for	for	IN
cord-014597-66vd2mdu	1423	14	routine	routine	JJ
cord-014597-66vd2mdu	1423	15	use	use	NN
cord-014597-66vd2mdu	1423	16	,	,	,
cord-014597-66vd2mdu	1423	17	it	-PRON-	PRP
cord-014597-66vd2mdu	1423	18	remains	remain	VBZ
cord-014597-66vd2mdu	1423	19	a	a	DT
cord-014597-66vd2mdu	1423	20	viable	viable	JJ
cord-014597-66vd2mdu	1423	21	cloning	cloning	NN
cord-014597-66vd2mdu	1423	22	method	method	NN
cord-014597-66vd2mdu	1423	23	.	.	.
cord-014597-66vd2mdu	1424	1	-Performed	-performe	VBN
cord-014597-66vd2mdu	1424	2	by	by	IN
cord-014597-66vd2mdu	1424	3	trained	train	VBN
cord-014597-66vd2mdu	1424	4	scientists	scientist	NNS
cord-014597-66vd2mdu	1424	5	-Dilute	-dilute	VBP
cord-014597-66vd2mdu	1424	6	culture	culture	NN
cord-014597-66vd2mdu	1424	7	to	to	IN
cord-014597-66vd2mdu	1424	8	1500	1500	CD
cord-014597-66vd2mdu	1424	9	±	±	NNP
cord-014597-66vd2mdu	1424	10	500	500	CD
cord-014597-66vd2mdu	1424	11	cells	cell	NNS
cord-014597-66vd2mdu	1424	12	/	/	SYM
cord-014597-66vd2mdu	1424	13	mL	ml	NN
cord-014597-66vd2mdu	1424	14	with	with	IN
cord-014597-66vd2mdu	1424	15	≤2	≤2	NNP
cord-014597-66vd2mdu	1424	16	%	%	NN
cord-014597-66vd2mdu	1424	17	doublets	doublet	NNS
cord-014597-66vd2mdu	1424	18	-Draw	-Draw	NNP
cord-014597-66vd2mdu	1424	19	cell	cell	NN
cord-014597-66vd2mdu	1424	20	suspension	suspension	NN
cord-014597-66vd2mdu	1424	21	into	into	IN
cord-014597-66vd2mdu	1424	22	pipette	pipette	NN
cord-014597-66vd2mdu	1424	23	tip	tip	NN
cord-014597-66vd2mdu	1424	24	by	by	IN
cord-014597-66vd2mdu	1424	25	capillary	capillary	JJ
cord-014597-66vd2mdu	1424	26	action	action	NN
cord-014597-66vd2mdu	1424	27	;	;	:
cord-014597-66vd2mdu	1424	28	tap	tap	NN
cord-014597-66vd2mdu	1424	29	tip	tip	NN
cord-014597-66vd2mdu	1424	30	against	against	IN
cord-014597-66vd2mdu	1424	31	the	the	DT
cord-014597-66vd2mdu	1424	32	centre	centre	NN
cord-014597-66vd2mdu	1424	33	of	of	IN
cord-014597-66vd2mdu	1424	34	the	the	DT
cord-014597-66vd2mdu	1424	35	base	base	NN
cord-014597-66vd2mdu	1424	36	of	of	IN
cord-014597-66vd2mdu	1424	37	each	each	DT
cord-014597-66vd2mdu	1424	38	well	well	NN
cord-014597-66vd2mdu	1424	39	of	of	IN
cord-014597-66vd2mdu	1424	40	a	a	DT
cord-014597-66vd2mdu	1424	41	48	48	CD
cord-014597-66vd2mdu	1424	42	well	well	JJ
cord-014597-66vd2mdu	1424	43	plate	plate	NN
cord-014597-66vd2mdu	1424	44	.	.	.
cord-014597-66vd2mdu	1425	1	-Size	-Size	NNP
cord-014597-66vd2mdu	1426	1	of	of	IN
cord-014597-66vd2mdu	1426	2	resulting	result	VBG
cord-014597-66vd2mdu	1426	3	droplet	droplet	NN
cord-014597-66vd2mdu	1426	4	=	=	NFP
cord-014597-66vd2mdu	1426	5	~1μL	~1μl	XX
cord-014597-66vd2mdu	1427	1	(	(	-LRB-
cord-014597-66vd2mdu	1427	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1427	3	.	.	.
cord-014597-66vd2mdu	1427	4	1a	1a	CD
cord-014597-66vd2mdu	1427	5	)	)	-RRB-
cord-014597-66vd2mdu	1428	1	-Two	-Two	.
cord-014597-66vd2mdu	1429	1	scientists	scientist	NNS
cord-014597-66vd2mdu	1429	2	independently	independently	RB
cord-014597-66vd2mdu	1429	3	view	view	VBP
cord-014597-66vd2mdu	1429	4	all	all	DT
cord-014597-66vd2mdu	1429	5	wells	well	NNS
cord-014597-66vd2mdu	1429	6	using	use	VBG
cord-014597-66vd2mdu	1429	7	a	a	DT
cord-014597-66vd2mdu	1429	8	microscope	microscope	NN
cord-014597-66vd2mdu	1429	9	(	(	-LRB-
cord-014597-66vd2mdu	1429	10	initially	initially	RB
cord-014597-66vd2mdu	1429	11	use	use	VB
cord-014597-66vd2mdu	1429	12	40x	40x	CD
cord-014597-66vd2mdu	1429	13	magnification	magnification	NN
cord-014597-66vd2mdu	1429	14	with	with	IN
cord-014597-66vd2mdu	1429	15	the	the	DT
cord-014597-66vd2mdu	1429	16	entire	entire	JJ
cord-014597-66vd2mdu	1429	17	rim	rim	NN
cord-014597-66vd2mdu	1429	18	of	of	IN
cord-014597-66vd2mdu	1429	19	the	the	DT
cord-014597-66vd2mdu	1429	20	droplet	droplet	NN
cord-014597-66vd2mdu	1429	21	visible	visible	JJ
cord-014597-66vd2mdu	1429	22	within	within	IN
cord-014597-66vd2mdu	1429	23	the	the	DT
cord-014597-66vd2mdu	1429	24	field	field	NN
cord-014597-66vd2mdu	1429	25	-	-	HYPH
cord-014597-66vd2mdu	1429	26	of	of	IN
cord-014597-66vd2mdu	1429	27	-	-	HYPH
cord-014597-66vd2mdu	1429	28	view	view	NN
cord-014597-66vd2mdu	1429	29	.	.	.
cord-014597-66vd2mdu	1430	1	Next	next	RB
cord-014597-66vd2mdu	1430	2	,	,	,
cord-014597-66vd2mdu	1430	3	examine	examine	VB
cord-014597-66vd2mdu	1430	4	particles	particle	NNS
cord-014597-66vd2mdu	1430	5	using	use	VBG
cord-014597-66vd2mdu	1430	6	100x	100x	JJR
cord-014597-66vd2mdu	1430	7	or	or	CC
cord-014597-66vd2mdu	1430	8	200x	200x	CD
cord-014597-66vd2mdu	1430	9	magnification	magnification	NN
cord-014597-66vd2mdu	1430	10	to	to	TO
cord-014597-66vd2mdu	1430	11	confirm	confirm	VB
cord-014597-66vd2mdu	1430	12	they	-PRON-	PRP
cord-014597-66vd2mdu	1430	13	are	be	VBP
cord-014597-66vd2mdu	1430	14	cells	cell	NNS
cord-014597-66vd2mdu	1430	15	)	)	-RRB-
cord-014597-66vd2mdu	1430	16	and	and	CC
cord-014597-66vd2mdu	1430	17	individually	individually	RB
cord-014597-66vd2mdu	1430	18	record	record	VB
cord-014597-66vd2mdu	1430	19	the	the	DT
cord-014597-66vd2mdu	1430	20	number	number	NN
cord-014597-66vd2mdu	1430	21	of	of	IN
cord-014597-66vd2mdu	1430	22	cells	cell	NNS
cord-014597-66vd2mdu	1430	23	present	present	JJ
cord-014597-66vd2mdu	1430	24	in	in	IN
cord-014597-66vd2mdu	1430	25	each	each	DT
cord-014597-66vd2mdu	1430	26	well	well	NN
cord-014597-66vd2mdu	1430	27	's	's	POS
cord-014597-66vd2mdu	1430	28	droplet	droplet	NN
cord-014597-66vd2mdu	1430	29	(	(	-LRB-
cord-014597-66vd2mdu	1430	30	Fig	fig	NN
cord-014597-66vd2mdu	1430	31	.	.	.
cord-014597-66vd2mdu	1431	1	1b	1b	CD
cord-014597-66vd2mdu	1431	2	to	to	IN
cord-014597-66vd2mdu	1431	3	d	d	NN
cord-014597-66vd2mdu	1431	4	)	)	-RRB-
cord-014597-66vd2mdu	1431	5	.	.	.
cord-014597-66vd2mdu	1432	1	-Exclude	-exclude	IN
cord-014597-66vd2mdu	1432	2	droplet	droplet	NN
cord-014597-66vd2mdu	1432	3	from	from	IN
cord-014597-66vd2mdu	1432	4	further	further	JJ
cord-014597-66vd2mdu	1432	5	analysis	analysis	NN
cord-014597-66vd2mdu	1432	6	if	if	IN
cord-014597-66vd2mdu	1432	7	full	full	JJ
cord-014597-66vd2mdu	1432	8	visualisation	visualisation	NN
cord-014597-66vd2mdu	1432	9	is	be	VBZ
cord-014597-66vd2mdu	1432	10	hindered	hinder	VBN
cord-014597-66vd2mdu	1432	11	(	(	-LRB-
cord-014597-66vd2mdu	1432	12	Fig	Fig	NNP
cord-014597-66vd2mdu	1432	13	.	.	.
cord-014597-66vd2mdu	1432	14	1e	1e	NNP
cord-014597-66vd2mdu	1432	15	to	to	IN
cord-014597-66vd2mdu	1432	16	h	h	NNP
cord-014597-66vd2mdu	1432	17	)	)	-RRB-
cord-014597-66vd2mdu	1432	18	.	.	.
cord-014597-66vd2mdu	1433	1	-Add	-add	DT
cord-014597-66vd2mdu	1433	2	growth	growth	NN
cord-014597-66vd2mdu	1433	3	medium	medium	NN
cord-014597-66vd2mdu	1433	4	,	,	,
cord-014597-66vd2mdu	1433	5	and	and	CC
cord-014597-66vd2mdu	1433	6	incubate	incubate	VB
cord-014597-66vd2mdu	1433	7	plates	plate	NNS
cord-014597-66vd2mdu	1433	8	.	.	.
cord-014597-66vd2mdu	1434	1	Record	record	VB
cord-014597-66vd2mdu	1434	2	all	all	DT
cord-014597-66vd2mdu	1434	3	wells	well	NNS
cord-014597-66vd2mdu	1434	4	containing	contain	VBG
cord-014597-66vd2mdu	1434	5	colonies	colony	NNS
cord-014597-66vd2mdu	1434	6	;	;	:
cord-014597-66vd2mdu	1434	7	only	only	RB
cord-014597-66vd2mdu	1434	8	progress	progress	NN
cord-014597-66vd2mdu	1434	9	colonies	colony	NNS
cord-014597-66vd2mdu	1434	10	from	from	IN
cord-014597-66vd2mdu	1434	11	wells	well	NNS
cord-014597-66vd2mdu	1434	12	that	that	WDT
cord-014597-66vd2mdu	1434	13	both	both	DT
cord-014597-66vd2mdu	1434	14	scientists	scientist	NNS
cord-014597-66vd2mdu	1434	15	agree	agree	VBP
cord-014597-66vd2mdu	1434	16	contains	contain	VBZ
cord-014597-66vd2mdu	1434	17	only	only	RB
cord-014597-66vd2mdu	1434	18	one	one	CD
cord-014597-66vd2mdu	1434	19	cell	cell	NN
cord-014597-66vd2mdu	1434	20	.	.	.
cord-014597-66vd2mdu	1435	1	-Data	-data	NN
cord-014597-66vd2mdu	1436	1	analysis	analysis	NN
cord-014597-66vd2mdu	1436	2	:	:	:
cord-014597-66vd2mdu	1437	1	-Each	-Each	.
cord-014597-66vd2mdu	1438	1	scientist	scientist	NN
cord-014597-66vd2mdu	1438	2	's	's	POS
cord-014597-66vd2mdu	1438	3	observations	observation	NNS
cord-014597-66vd2mdu	1438	4	categorised	categorise	VBN
cord-014597-66vd2mdu	1438	5	as	as	IN
cord-014597-66vd2mdu	1438	6	:	:	:
cord-014597-66vd2mdu	1438	7	0	0	CD
cord-014597-66vd2mdu	1438	8	cells	cell	NNS
cord-014597-66vd2mdu	1438	9	,	,	,
cord-014597-66vd2mdu	1438	10	1	1	CD
cord-014597-66vd2mdu	1438	11	cell	cell	NN
cord-014597-66vd2mdu	1438	12	or	or	CC
cord-014597-66vd2mdu	1438	13	>	>	NN
cord-014597-66vd2mdu	1438	14	1	1	CD
cord-014597-66vd2mdu	1438	15	cell	cell	NN
cord-014597-66vd2mdu	1438	16	-Observed	-observe	VBD
cord-014597-66vd2mdu	1438	17	outcome	outcome	NN
cord-014597-66vd2mdu	1438	18	for	for	IN
cord-014597-66vd2mdu	1438	19	each	each	DT
cord-014597-66vd2mdu	1438	20	well	well	NN
cord-014597-66vd2mdu	1438	21	:	:	:
cord-014597-66vd2mdu	1438	22	growth	growth	NN
cord-014597-66vd2mdu	1438	23	or	or	CC
cord-014597-66vd2mdu	1438	24	no	no	DT
cord-014597-66vd2mdu	1438	25	growth	growth	NN
cord-014597-66vd2mdu	1438	26	-Probability	-probability	NN
cord-014597-66vd2mdu	1438	27	of	of	IN
cord-014597-66vd2mdu	1438	28	monoclonality	monoclonality	NN
cord-014597-66vd2mdu	1438	29	estimated	estimate	VBN
cord-014597-66vd2mdu	1438	30	from	from	IN
cord-014597-66vd2mdu	1438	31	data	datum	NNS
cord-014597-66vd2mdu	1438	32	using	use	VBG
cord-014597-66vd2mdu	1438	33	a	a	DT
cord-014597-66vd2mdu	1438	34	statistical	statistical	JJ
cord-014597-66vd2mdu	1438	35	model	model	NN
cord-014597-66vd2mdu	1438	36	Cloning	clone	VBG
cord-014597-66vd2mdu	1438	37	(	(	-LRB-
cord-014597-66vd2mdu	1438	38	LDC	LDC	NNP
cord-014597-66vd2mdu	1438	39	)	)	-RRB-
cord-014597-66vd2mdu	1439	1	Increased	increased	JJ
cord-014597-66vd2mdu	1439	2	accuracy	accuracy	NN
cord-014597-66vd2mdu	1439	3	of	of	IN
cord-014597-66vd2mdu	1439	4	P(monoclonality	P(monoclonality	NNP
cord-014597-66vd2mdu	1439	5	)	)	-RRB-
cord-014597-66vd2mdu	1439	6	with	with	IN
cord-014597-66vd2mdu	1439	7	CACC	CACC	NNP
cord-014597-66vd2mdu	1439	8	-LDC	-LDC	JJS
cord-014597-66vd2mdu	1439	9	weakness	weakness	NN
cord-014597-66vd2mdu	1439	10	:	:	:
cord-014597-66vd2mdu	1439	11	no	no	DT
cord-014597-66vd2mdu	1439	12	visualisation	visualisation	NN
cord-014597-66vd2mdu	1439	13	after	after	IN
cord-014597-66vd2mdu	1439	14	seeding	seed	VBG
cord-014597-66vd2mdu	1439	15	(	(	-LRB-
cord-014597-66vd2mdu	1439	16	to	to	TO
cord-014597-66vd2mdu	1439	17	check	check	VB
cord-014597-66vd2mdu	1439	18	both	both	DT
cord-014597-66vd2mdu	1439	19	well	well	RB
cord-014597-66vd2mdu	1439	20	seeding	seed	VBG
cord-014597-66vd2mdu	1439	21	and	and	CC
cord-014597-66vd2mdu	1439	22	subsequent	subsequent	JJ
cord-014597-66vd2mdu	1439	23	growth	growth	NN
cord-014597-66vd2mdu	1439	24	of	of	IN
cord-014597-66vd2mdu	1439	25	colonies	colony	NNS
cord-014597-66vd2mdu	1439	26	is	be	VBZ
cord-014597-66vd2mdu	1439	27	well	well	RB
cord-014597-66vd2mdu	1439	28	described	describe	VBN
cord-014597-66vd2mdu	1439	29	by	by	IN
cord-014597-66vd2mdu	1439	30	the	the	DT
cord-014597-66vd2mdu	1439	31	Poisson	Poisson	NNP
cord-014597-66vd2mdu	1439	32	distribution	distribution	NN
cord-014597-66vd2mdu	1439	33	)	)	-RRB-
cord-014597-66vd2mdu	1439	34	,	,	,
cord-014597-66vd2mdu	1439	35	potentially	potentially	RB
cord-014597-66vd2mdu	1439	36	overestimating	overestimate	VBG
cord-014597-66vd2mdu	1439	37	P(monoclonality	p(monoclonality	NN
cord-014597-66vd2mdu	1439	38	)	)	-RRB-
cord-014597-66vd2mdu	1440	1	-Addressed	-addresse	VBN
cord-014597-66vd2mdu	1441	1	by	by	IN
cord-014597-66vd2mdu	1441	2	CACC	CACC	NNP
cord-014597-66vd2mdu	1441	3	:	:	:
cord-014597-66vd2mdu	1441	4	Visual	visual	JJ
cord-014597-66vd2mdu	1441	5	examination	examination	NN
cord-014597-66vd2mdu	1441	6	with	with	IN
cord-014597-66vd2mdu	1441	7	colonies	colony	NNS
cord-014597-66vd2mdu	1441	8	arising	arise	VBG
cord-014597-66vd2mdu	1441	9	from	from	IN
cord-014597-66vd2mdu	1441	10	wells	well	NNS
cord-014597-66vd2mdu	1441	11	seeded	seed	VBN
cord-014597-66vd2mdu	1441	12	with	with	IN
cord-014597-66vd2mdu	1441	13	1	1	CD
cord-014597-66vd2mdu	1441	14	cell	cell	NN
cord-014597-66vd2mdu	1441	15	distinguished	distinguish	VBN
cord-014597-66vd2mdu	1441	16	from	from	IN
cord-014597-66vd2mdu	1441	17	those	those	DT
cord-014597-66vd2mdu	1441	18	seeded	seed	VBN
cord-014597-66vd2mdu	1441	19	with	with	IN
cord-014597-66vd2mdu	1441	20	>	>	XX
cord-014597-66vd2mdu	1441	21	1	1	CD
cord-014597-66vd2mdu	1441	22	cell	cell	NN
cord-014597-66vd2mdu	1441	23	-Visualisation	-visualisation	NN
cord-014597-66vd2mdu	1441	24	step	step	NN
cord-014597-66vd2mdu	1441	25	further	far	RBR
cord-014597-66vd2mdu	1441	26	strengthened	strengthen	VBN
cord-014597-66vd2mdu	1441	27	by	by	IN
cord-014597-66vd2mdu	1441	28	:	:	:
cord-014597-66vd2mdu	1441	29	Using	use	VBG
cord-014597-66vd2mdu	1441	30	controls	control	NNS
cord-014597-66vd2mdu	1441	31	for	for	IN
cord-014597-66vd2mdu	1441	32	exclusion	exclusion	NN
cord-014597-66vd2mdu	1441	33	of	of	IN
cord-014597-66vd2mdu	1441	34	wells	well	NNS
cord-014597-66vd2mdu	1441	35	;	;	:
cord-014597-66vd2mdu	1441	36	measuring	measure	VBG
cord-014597-66vd2mdu	1441	37	errors	error	NNS
cord-014597-66vd2mdu	1441	38	based	base	VBN
cord-014597-66vd2mdu	1441	39	on	on	IN
cord-014597-66vd2mdu	1441	40	the	the	DT
cord-014597-66vd2mdu	1441	41	presence	presence	NN
cord-014597-66vd2mdu	1441	42	or	or	CC
cord-014597-66vd2mdu	1441	43	absence	absence	NN
cord-014597-66vd2mdu	1441	44	of	of	IN
cord-014597-66vd2mdu	1441	45	colonies	colony	NNS
cord-014597-66vd2mdu	1441	46	in	in	IN
cord-014597-66vd2mdu	1441	47	wells	well	NNS
cord-014597-66vd2mdu	1441	48	where	where	WRB
cord-014597-66vd2mdu	1441	49	two	two	CD
cord-014597-66vd2mdu	1441	50	scientists	scientist	NNS
cord-014597-66vd2mdu	1441	51	independently	independently	RB
cord-014597-66vd2mdu	1441	52	reported	report	VBD
cord-014597-66vd2mdu	1441	53	0	0	CD
cord-014597-66vd2mdu	1441	54	cells	cell	NNS
cord-014597-66vd2mdu	1441	55	;	;	:
cord-014597-66vd2mdu	1441	56	and	and	CC
cord-014597-66vd2mdu	1441	57	formally	formally	RB
cord-014597-66vd2mdu	1441	58	analysing	analyse	VBG
cord-014597-66vd2mdu	1441	59	the	the	DT
cord-014597-66vd2mdu	1441	60	data	datum	NNS
cord-014597-66vd2mdu	1441	61	using	use	VBG
cord-014597-66vd2mdu	1441	62	a	a	DT
cord-014597-66vd2mdu	1441	63	suitable	suitable	JJ
cord-014597-66vd2mdu	1441	64	statistical	statistical	JJ
cord-014597-66vd2mdu	1441	65	model	model	NN
cord-014597-66vd2mdu	1441	66	Decreased	decrease	VBD
cord-014597-66vd2mdu	1441	67	time	time	NN
cord-014597-66vd2mdu	1441	68	and	and	CC
cord-014597-66vd2mdu	1441	69	resource	resource	NN
cord-014597-66vd2mdu	1441	70	requirements	requirement	NNS
cord-014597-66vd2mdu	1441	71	with	with	IN
cord-014597-66vd2mdu	1441	72	CACC	CACC	NNP
cord-014597-66vd2mdu	1441	73	-High	-High	NNP
cord-014597-66vd2mdu	1441	74	P(monoclonality	P(monoclonality	NNP
cord-014597-66vd2mdu	1441	75	)	)	-RRB-
cord-014597-66vd2mdu	1441	76	possible	possible	JJ
cord-014597-66vd2mdu	1441	77	in	in	IN
cord-014597-66vd2mdu	1441	78	single	single	JJ
cord-014597-66vd2mdu	1441	79	round	round	NN
cord-014597-66vd2mdu	1441	80	as	as	IN
cord-014597-66vd2mdu	1441	81	each	each	DT
cord-014597-66vd2mdu	1441	82	well	well	RB
cord-014597-66vd2mdu	1441	83	examined	examine	VBN
cord-014597-66vd2mdu	1441	84	individually	individually	RB
cord-014597-66vd2mdu	1441	85	with	with	IN
cord-014597-66vd2mdu	1441	86	only	only	RB
cord-014597-66vd2mdu	1441	87	those	those	DT
cord-014597-66vd2mdu	1441	88	containing	contain	VBG
cord-014597-66vd2mdu	1441	89	a	a	DT
cord-014597-66vd2mdu	1441	90	single	single	JJ
cord-014597-66vd2mdu	1441	91	cell	cell	NN
cord-014597-66vd2mdu	1441	92	progressed	progress	VBD
cord-014597-66vd2mdu	1441	93	,	,	,
cord-014597-66vd2mdu	1441	94	and	and	CC
cord-014597-66vd2mdu	1442	1	because	because	IN
cord-014597-66vd2mdu	1442	2	the	the	DT
cord-014597-66vd2mdu	1442	3	error	error	NN
cord-014597-66vd2mdu	1442	4	rate	rate	NN
cord-014597-66vd2mdu	1442	5	for	for	IN
cord-014597-66vd2mdu	1442	6	incorrect	incorrect	JJ
cord-014597-66vd2mdu	1442	7	scoring	scoring	NN
cord-014597-66vd2mdu	1442	8	is	be	VBZ
cord-014597-66vd2mdu	1442	9	considered	consider	VBN
cord-014597-66vd2mdu	1442	10	to	to	TO
cord-014597-66vd2mdu	1442	11	be	be	VB
cord-014597-66vd2mdu	1442	12	low	low	JJ
cord-014597-66vd2mdu	1442	13	two	two	CD
cord-014597-66vd2mdu	1442	14	scientists	scientist	NNS
cord-014597-66vd2mdu	1442	15	miss	miss	VBP
cord-014597-66vd2mdu	1442	16	a	a	DT
cord-014597-66vd2mdu	1442	17	cell	cell	NN
cord-014597-66vd2mdu	1442	18	one	one	CD
cord-014597-66vd2mdu	1442	19	cell	cell	NN
cord-014597-66vd2mdu	1442	20	sitting	sit	VBG
cord-014597-66vd2mdu	1442	21	on	on	IN
cord-014597-66vd2mdu	1442	22	top	top	NN
cord-014597-66vd2mdu	1442	23	of	of	IN
cord-014597-66vd2mdu	1442	24	another	another	DT
cord-014597-66vd2mdu	1442	25	and	and	CC
cord-014597-66vd2mdu	1442	26	the	the	DT
cord-014597-66vd2mdu	1442	27	two	two	CD
cord-014597-66vd2mdu	1442	28	thus	thus	RB
cord-014597-66vd2mdu	1442	29	appearing	appear	VBG
cord-014597-66vd2mdu	1442	30	as	as	IN
cord-014597-66vd2mdu	1442	31	one	one	CD
cord-014597-66vd2mdu	1442	32	An	an	DT
cord-014597-66vd2mdu	1442	33	experiment	experiment	NN
cord-014597-66vd2mdu	1442	34	was	be	VBD
cord-014597-66vd2mdu	1442	35	performed	perform	VBN
cord-014597-66vd2mdu	1442	36	to	to	TO
cord-014597-66vd2mdu	1442	37	estimate	estimate	VB
cord-014597-66vd2mdu	1442	38	error	error	NN
cord-014597-66vd2mdu	1442	39	frequency	frequency	NN
cord-014597-66vd2mdu	1442	40	[	[	-LRB-
cord-014597-66vd2mdu	1442	41	3	3	CD
cord-014597-66vd2mdu	1442	42	]	]	-RRB-
cord-014597-66vd2mdu	1442	43	.	.	.
cord-014597-66vd2mdu	1443	1	Conclusion	conclusion	NN
cord-014597-66vd2mdu	1443	2	-Scientists	-scientist	NNS
cord-014597-66vd2mdu	1443	3	miss	miss	VB
cord-014597-66vd2mdu	1443	4	a	a	DT
cord-014597-66vd2mdu	1443	5	cell	cell	NN
cord-014597-66vd2mdu	1443	6	infrequently	infrequently	RB
cord-014597-66vd2mdu	1443	7	(	(	-LRB-
cord-014597-66vd2mdu	1443	8	in	in	IN
cord-014597-66vd2mdu	1443	9	the	the	DT
cord-014597-66vd2mdu	1443	10	range	range	NN
cord-014597-66vd2mdu	1443	11	0.4	0.4	CD
cord-014597-66vd2mdu	1443	12	%	%	NN
cord-014597-66vd2mdu	1443	13	to	to	IN
cord-014597-66vd2mdu	1443	14	1.3	1.3	CD
cord-014597-66vd2mdu	1443	15	%	%	NN
cord-014597-66vd2mdu	1443	16	,	,	,
cord-014597-66vd2mdu	1443	17	[	[	-LRB-
cord-014597-66vd2mdu	1443	18	3	3	CD
cord-014597-66vd2mdu	1443	19	]	]	-RRB-
cord-014597-66vd2mdu	1443	20	)	)	-RRB-
cord-014597-66vd2mdu	1444	1	-Error	-Error	:
cord-014597-66vd2mdu	1444	2	frequency	frequency	NN
cord-014597-66vd2mdu	1444	3	does	do	VBZ
cord-014597-66vd2mdu	1444	4	not	not	RB
cord-014597-66vd2mdu	1444	5	invalidate	invalidate	VB
cord-014597-66vd2mdu	1444	6	use	use	NN
cord-014597-66vd2mdu	1444	7	of	of	IN
cord-014597-66vd2mdu	1444	8	direct	direct	JJ
cord-014597-66vd2mdu	1444	9	observation	observation	NN
cord-014597-66vd2mdu	1444	10	methods	method	NNS
cord-014597-66vd2mdu	1444	11	for	for	IN
cord-014597-66vd2mdu	1444	12	cell	cell	NN
cord-014597-66vd2mdu	1444	13	cloning	cloning	NN
cord-014597-66vd2mdu	1444	14	-Single	-single	IN
cord-014597-66vd2mdu	1444	15	cell	cell	NN
cord-014597-66vd2mdu	1444	16	seen	see	VBN
cord-014597-66vd2mdu	1444	17	by	by	IN
cord-014597-66vd2mdu	1444	18	both	both	DT
cord-014597-66vd2mdu	1444	19	scientists	scientist	NNS
cord-014597-66vd2mdu	1444	20	is	be	VBZ
cord-014597-66vd2mdu	1444	21	highly	highly	RB
cord-014597-66vd2mdu	1444	22	likely	likely	JJ
cord-014597-66vd2mdu	1444	23	to	to	TO
cord-014597-66vd2mdu	1444	24	be	be	VB
cord-014597-66vd2mdu	1444	25	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1444	26	-During	-during	NN
cord-014597-66vd2mdu	1444	27	method	method	NN
cord-014597-66vd2mdu	1444	28	development	development	NN
cord-014597-66vd2mdu	1444	29	,	,	,
cord-014597-66vd2mdu	1444	30	strategies	strategy	NNS
cord-014597-66vd2mdu	1444	31	established	establish	VBN
cord-014597-66vd2mdu	1444	32	to	to	TO
cord-014597-66vd2mdu	1444	33	control	control	VB
cord-014597-66vd2mdu	1444	34	potential	potential	JJ
cord-014597-66vd2mdu	1444	35	sources	source	NNS
cord-014597-66vd2mdu	1444	36	of	of	IN
cord-014597-66vd2mdu	1444	37	error	error	NN
cord-014597-66vd2mdu	1444	38	(	(	-LRB-
cord-014597-66vd2mdu	1444	39	Table	table	NN
cord-014597-66vd2mdu	1444	40	1	1	CD
cord-014597-66vd2mdu	1444	41	)	)	-RRB-
cord-014597-66vd2mdu	1444	42	Use	use	NN
cord-014597-66vd2mdu	1444	43	of	of	IN
cord-014597-66vd2mdu	1444	44	a	a	DT
cord-014597-66vd2mdu	1444	45	contemporaneous	contemporaneous	JJ
cord-014597-66vd2mdu	1444	46	visualisation	visualisation	NN
cord-014597-66vd2mdu	1444	47	approach	approach	NN
cord-014597-66vd2mdu	1444	48	,	,	,
cord-014597-66vd2mdu	1444	49	a	a	DT
cord-014597-66vd2mdu	1444	50	strict	strict	JJ
cord-014597-66vd2mdu	1444	51	control	control	NN
cord-014597-66vd2mdu	1444	52	strategy	strategy	NN
cord-014597-66vd2mdu	1444	53	,	,	,
cord-014597-66vd2mdu	1444	54	and	and	CC
cord-014597-66vd2mdu	1444	55	a	a	DT
cord-014597-66vd2mdu	1444	56	suitable	suitable	JJ
cord-014597-66vd2mdu	1444	57	statistical	statistical	JJ
cord-014597-66vd2mdu	1444	58	model	model	NN
cord-014597-66vd2mdu	1444	59	(	(	-LRB-
cord-014597-66vd2mdu	1444	60	which	which	WDT
cord-014597-66vd2mdu	1444	61	takes	take	VBZ
cord-014597-66vd2mdu	1444	62	into	into	IN
cord-014597-66vd2mdu	1444	63	account	account	NN
cord-014597-66vd2mdu	1444	64	potential	potential	JJ
cord-014597-66vd2mdu	1444	65	errors	error	NNS
cord-014597-66vd2mdu	1444	66	)	)	-RRB-
cord-014597-66vd2mdu	1444	67	results	result	VBZ
cord-014597-66vd2mdu	1444	68	in	in	IN
cord-014597-66vd2mdu	1444	69	:	:	:
cord-014597-66vd2mdu	1444	70	-The	-The	:
cord-014597-66vd2mdu	1444	71	CACC	CACC	NNP
cord-014597-66vd2mdu	1444	72	method	method	NN
cord-014597-66vd2mdu	1444	73	being	be	VBG
cord-014597-66vd2mdu	1444	74	at	at	RB
cord-014597-66vd2mdu	1444	75	least	least	JJS
cord-014597-66vd2mdu	1444	76	as	as	RB
cord-014597-66vd2mdu	1444	77	robust	robust	JJ
cord-014597-66vd2mdu	1444	78	as	as	IN
cord-014597-66vd2mdu	1444	79	the	the	DT
cord-014597-66vd2mdu	1444	80	LDC	LDC	NNP
cord-014597-66vd2mdu	1444	81	method	method	NN
cord-014597-66vd2mdu	1444	82	-The	-The	:
cord-014597-66vd2mdu	1444	83	CACC	CACC	NNP
cord-014597-66vd2mdu	1444	84	method	method	NN
cord-014597-66vd2mdu	1444	85	being	be	VBG
cord-014597-66vd2mdu	1444	86	a	a	DT
cord-014597-66vd2mdu	1444	87	reliable	reliable	JJ
cord-014597-66vd2mdu	1444	88	,	,	,
cord-014597-66vd2mdu	1444	89	single	single	JJ
cord-014597-66vd2mdu	1444	90	-	-	HYPH
cord-014597-66vd2mdu	1444	91	step	step	NN
cord-014597-66vd2mdu	1444	92	method	method	NN
cord-014597-66vd2mdu	1444	93	for	for	IN
cord-014597-66vd2mdu	1444	94	cloning	clone	VBG
cord-014597-66vd2mdu	1444	95	to	to	TO
cord-014597-66vd2mdu	1444	96	achieve	achieve	VB
cord-014597-66vd2mdu	1444	97	a	a	DT
cord-014597-66vd2mdu	1444	98	high	high	JJ
cord-014597-66vd2mdu	1444	99	P(monoclonality	p(monoclonality	NN
cord-014597-66vd2mdu	1444	100	)	)	-RRB-
cord-014597-66vd2mdu	1445	1	Background	background	NN
cord-014597-66vd2mdu	1445	2	Vector	Vector	NNP
cord-014597-66vd2mdu	1445	3	design	design	NN
cord-014597-66vd2mdu	1445	4	is	be	VBZ
cord-014597-66vd2mdu	1445	5	a	a	DT
cord-014597-66vd2mdu	1445	6	key	key	JJ
cord-014597-66vd2mdu	1445	7	step	step	NN
cord-014597-66vd2mdu	1445	8	in	in	IN
cord-014597-66vd2mdu	1445	9	cell	cell	NN
cord-014597-66vd2mdu	1445	10	line	line	NN
cord-014597-66vd2mdu	1445	11	development	development	NN
cord-014597-66vd2mdu	1445	12	for	for	IN
cord-014597-66vd2mdu	1445	13	the	the	DT
cord-014597-66vd2mdu	1445	14	expression	expression	NN
cord-014597-66vd2mdu	1445	15	of	of	IN
cord-014597-66vd2mdu	1445	16	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1445	17	biologics	biologic	NNS
cord-014597-66vd2mdu	1445	18	.	.	.
cord-014597-66vd2mdu	1446	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1446	2	is	be	VBZ
cord-014597-66vd2mdu	1446	3	essential	essential	JJ
cord-014597-66vd2mdu	1446	4	that	that	IN
cord-014597-66vd2mdu	1446	5	the	the	DT
cord-014597-66vd2mdu	1446	6	vector	vector	NN
cord-014597-66vd2mdu	1446	7	design	design	NN
cord-014597-66vd2mdu	1446	8	results	result	NNS
cord-014597-66vd2mdu	1446	9	in	in	IN
cord-014597-66vd2mdu	1446	10	high	high	JJ
cord-014597-66vd2mdu	1446	11	,	,	,
cord-014597-66vd2mdu	1446	12	stable	stable	JJ
cord-014597-66vd2mdu	1446	13	expression	expression	NN
cord-014597-66vd2mdu	1446	14	of	of	IN
cord-014597-66vd2mdu	1446	15	the	the	DT
cord-014597-66vd2mdu	1446	16	encoded	encode	VBN
cord-014597-66vd2mdu	1446	17	protein	protein	NN
cord-014597-66vd2mdu	1446	18	.	.	.
cord-014597-66vd2mdu	1447	1	Other	other	JJ
cord-014597-66vd2mdu	1447	2	considerations	consideration	NNS
cord-014597-66vd2mdu	1447	3	include	include	VBP
cord-014597-66vd2mdu	1447	4	ease	ease	NN
cord-014597-66vd2mdu	1447	5	of	of	IN
cord-014597-66vd2mdu	1447	6	cloning	cloning	NN
cord-014597-66vd2mdu	1447	7	,	,	,
cord-014597-66vd2mdu	1447	8	stability	stability	NN
cord-014597-66vd2mdu	1447	9	for	for	IN
cord-014597-66vd2mdu	1447	10	propagation	propagation	NN
cord-014597-66vd2mdu	1447	11	in	in	IN
cord-014597-66vd2mdu	1447	12	E.	E.	NNP
cord-014597-66vd2mdu	1447	13	coli	coli	NNS
cord-014597-66vd2mdu	1447	14	as	as	RB
cord-014597-66vd2mdu	1447	15	well	well	RB
cord-014597-66vd2mdu	1447	16	as	as	IN
cord-014597-66vd2mdu	1447	17	in	in	IN
cord-014597-66vd2mdu	1447	18	the	the	DT
cord-014597-66vd2mdu	1447	19	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1447	20	host	host	NN
cord-014597-66vd2mdu	1447	21	cell	cell	NN
cord-014597-66vd2mdu	1447	22	line	line	NN
cord-014597-66vd2mdu	1447	23	,	,	,
cord-014597-66vd2mdu	1447	24	and	and	CC
cord-014597-66vd2mdu	1447	25	ease	ease	NN
cord-014597-66vd2mdu	1447	26	of	of	IN
cord-014597-66vd2mdu	1447	27	sequence	sequence	NN
cord-014597-66vd2mdu	1447	28	amplification	amplification	NN
cord-014597-66vd2mdu	1447	29	for	for	IN
cord-014597-66vd2mdu	1447	30	verification	verification	NN
cord-014597-66vd2mdu	1447	31	of	of	IN
cord-014597-66vd2mdu	1447	32	vector	vector	NN
cord-014597-66vd2mdu	1447	33	construction	construction	NN
cord-014597-66vd2mdu	1447	34	and	and	CC
cord-014597-66vd2mdu	1447	35	for	for	IN
cord-014597-66vd2mdu	1447	36	detection	detection	NN
cord-014597-66vd2mdu	1447	37	of	of	IN
cord-014597-66vd2mdu	1447	38	insertion	insertion	NN
cord-014597-66vd2mdu	1447	39	site	site	NN
cord-014597-66vd2mdu	1447	40	and	and	CC
cord-014597-66vd2mdu	1447	41	copy	copy	NN
cord-014597-66vd2mdu	1447	42	number	number	NN
cord-014597-66vd2mdu	1447	43	in	in	IN
cord-014597-66vd2mdu	1447	44	stably	stably	RB
cord-014597-66vd2mdu	1447	45	expressing	express	VBG
cord-014597-66vd2mdu	1447	46	cells	cell	NNS
cord-014597-66vd2mdu	1447	47	.	.	.
cord-014597-66vd2mdu	1448	1	For	for	IN
cord-014597-66vd2mdu	1448	2	these	these	DT
cord-014597-66vd2mdu	1448	3	reasons	reason	NNS
cord-014597-66vd2mdu	1448	4	,	,	,
cord-014597-66vd2mdu	1448	5	use	use	NN
cord-014597-66vd2mdu	1448	6	of	of	IN
cord-014597-66vd2mdu	1448	7	the	the	DT
cord-014597-66vd2mdu	1448	8	same	same	JJ
cord-014597-66vd2mdu	1448	9	promoters	promoter	NNS
cord-014597-66vd2mdu	1448	10	and	and	CC
cord-014597-66vd2mdu	1448	11	polyA	polyA	NNP
cord-014597-66vd2mdu	1448	12	tails	tail	NNS
cord-014597-66vd2mdu	1448	13	in	in	IN
cord-014597-66vd2mdu	1448	14	dual	dual	JJ
cord-014597-66vd2mdu	1448	15	cassette	cassette	NN
cord-014597-66vd2mdu	1448	16	vectors	vector	NNS
cord-014597-66vd2mdu	1448	17	,	,	,
cord-014597-66vd2mdu	1448	18	as	as	IN
cord-014597-66vd2mdu	1448	19	is	be	VBZ
cord-014597-66vd2mdu	1448	20	common	common	JJ
cord-014597-66vd2mdu	1448	21	for	for	IN
cord-014597-66vd2mdu	1448	22	expression	expression	NN
cord-014597-66vd2mdu	1448	23	of	of	IN
cord-014597-66vd2mdu	1448	24	the	the	DT
cord-014597-66vd2mdu	1448	25	heavy	heavy	JJ
cord-014597-66vd2mdu	1448	26	and	and	CC
cord-014597-66vd2mdu	1448	27	light	light	JJ
cord-014597-66vd2mdu	1448	28	chains	chain	NNS
cord-014597-66vd2mdu	1448	29	of	of	IN
cord-014597-66vd2mdu	1448	30	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1448	31	antibodies	antibody	NNS
cord-014597-66vd2mdu	1448	32	,	,	,
cord-014597-66vd2mdu	1448	33	can	can	MD
cord-014597-66vd2mdu	1448	34	be	be	VB
cord-014597-66vd2mdu	1448	35	problematic	problematic	JJ
cord-014597-66vd2mdu	1448	36	.	.	.
cord-014597-66vd2mdu	1449	1	In	in	IN
cord-014597-66vd2mdu	1449	2	order	order	NN
cord-014597-66vd2mdu	1449	3	to	to	TO
cord-014597-66vd2mdu	1449	4	minimize	minimize	VB
cord-014597-66vd2mdu	1449	5	sequence	sequence	NN
cord-014597-66vd2mdu	1449	6	similarities	similarity	NNS
cord-014597-66vd2mdu	1449	7	between	between	IN
cord-014597-66vd2mdu	1449	8	the	the	DT
cord-014597-66vd2mdu	1449	9	two	two	CD
cord-014597-66vd2mdu	1449	10	expression	expression	NN
cord-014597-66vd2mdu	1449	11	cassettes	cassette	NNS
cord-014597-66vd2mdu	1449	12	,	,	,
cord-014597-66vd2mdu	1449	13	we	-PRON-	PRP
cord-014597-66vd2mdu	1449	14	have	have	VBP
cord-014597-66vd2mdu	1449	15	modified	modify	VBN
cord-014597-66vd2mdu	1449	16	the	the	DT
cord-014597-66vd2mdu	1449	17	promoters	promoter	NNS
cord-014597-66vd2mdu	1449	18	,	,	,
cord-014597-66vd2mdu	1449	19	introns	intron	NNS
cord-014597-66vd2mdu	1449	20	,	,	,
cord-014597-66vd2mdu	1449	21	and	and	CC
cord-014597-66vd2mdu	1449	22	polyA	polyA	NNP
cord-014597-66vd2mdu	1449	23	tails	tail	NNS
cord-014597-66vd2mdu	1449	24	of	of	IN
cord-014597-66vd2mdu	1449	25	the	the	DT
cord-014597-66vd2mdu	1449	26	light	light	JJ
cord-014597-66vd2mdu	1449	27	chain	chain	NN
cord-014597-66vd2mdu	1449	28	and	and	CC
cord-014597-66vd2mdu	1449	29	heavy	heavy	JJ
cord-014597-66vd2mdu	1449	30	chain	chain	NN
cord-014597-66vd2mdu	1449	31	expression	expression	NN
cord-014597-66vd2mdu	1449	32	cassettes	cassette	NNS
cord-014597-66vd2mdu	1449	33	in	in	IN
cord-014597-66vd2mdu	1449	34	the	the	DT
cord-014597-66vd2mdu	1449	35	dual	dual	JJ
cord-014597-66vd2mdu	1449	36	expression	expression	NN
cord-014597-66vd2mdu	1449	37	vector	vector	NN
cord-014597-66vd2mdu	1449	38	commonly	commonly	RB
cord-014597-66vd2mdu	1449	39	used	use	VBN
cord-014597-66vd2mdu	1449	40	for	for	IN
cord-014597-66vd2mdu	1449	41	the	the	DT
cord-014597-66vd2mdu	1449	42	expression	expression	NN
cord-014597-66vd2mdu	1449	43	of	of	IN
cord-014597-66vd2mdu	1449	44	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1449	45	antibodies	antibody	NNS
cord-014597-66vd2mdu	1449	46	in	in	IN
cord-014597-66vd2mdu	1449	47	the	the	DT
cord-014597-66vd2mdu	1449	48	CHOZN	CHOZN	NNP
cord-014597-66vd2mdu	1449	49	®	®	NNPS
cord-014597-66vd2mdu	1449	50	GS	GS	NNP
cord-014597-66vd2mdu	1449	51	-/cell	-/cell	NNP
cord-014597-66vd2mdu	1449	52	line	line	NNP
cord-014597-66vd2mdu	1449	53	development	development	NN
cord-014597-66vd2mdu	1449	54	platform	platform	NN
cord-014597-66vd2mdu	1449	55	.	.	.
cord-014597-66vd2mdu	1450	1	Gene	gene	NN
cord-014597-66vd2mdu	1450	2	synthesis	synthesis	NN
cord-014597-66vd2mdu	1450	3	and	and	CC
cord-014597-66vd2mdu	1450	4	vector	vector	NN
cord-014597-66vd2mdu	1450	5	construction	construction	NN
cord-014597-66vd2mdu	1450	6	of	of	IN
cord-014597-66vd2mdu	1450	7	IgG1	igg1	NN
cord-014597-66vd2mdu	1450	8	and	and	CC
cord-014597-66vd2mdu	1450	9	fluorophore	fluorophore	NN
cord-014597-66vd2mdu	1450	10	-	-	HYPH
cord-014597-66vd2mdu	1450	11	expressing	express	VBG
cord-014597-66vd2mdu	1450	12	vectors	vector	NNS
cord-014597-66vd2mdu	1450	13	was	be	VBD
cord-014597-66vd2mdu	1450	14	done	do	VBN
cord-014597-66vd2mdu	1450	15	by	by	IN
cord-014597-66vd2mdu	1450	16	Atum	Atum	NNP
cord-014597-66vd2mdu	1450	17	.	.	.
cord-014597-66vd2mdu	1451	1	Vectors	vector	NNS
cord-014597-66vd2mdu	1451	2	were	be	VBD
cord-014597-66vd2mdu	1451	3	transfected	transfecte	VBN
cord-014597-66vd2mdu	1451	4	into	into	IN
cord-014597-66vd2mdu	1451	5	CHOZN	CHOZN	NNP
cord-014597-66vd2mdu	1451	6	®	®	POS
cord-014597-66vd2mdu	1451	7	GS	GS	NNP
cord-014597-66vd2mdu	1451	8	-/cells	-/cell	VBZ
cord-014597-66vd2mdu	1451	9	via	via	IN
cord-014597-66vd2mdu	1451	10	electroporation	electroporation	NN
cord-014597-66vd2mdu	1451	11	.	.	.
cord-014597-66vd2mdu	1452	1	Analysis	Analysis	NNP
cord-014597-66vd2mdu	1452	2	of	of	IN
cord-014597-66vd2mdu	1452	3	GFP	GFP	NNP
cord-014597-66vd2mdu	1452	4	and	and	CC
cord-014597-66vd2mdu	1452	5	RFP	RFP	NNP
cord-014597-66vd2mdu	1452	6	expression	expression	NN
cord-014597-66vd2mdu	1452	7	was	be	VBD
cord-014597-66vd2mdu	1452	8	achieved	achieve	VBN
cord-014597-66vd2mdu	1452	9	using	use	VBG
cord-014597-66vd2mdu	1452	10	a	a	DT
cord-014597-66vd2mdu	1452	11	MACSQuant	macsquant	NN
cord-014597-66vd2mdu	1452	12	instrument	instrument	NN
cord-014597-66vd2mdu	1452	13	.	.	.
cord-014597-66vd2mdu	1453	1	Selection	selection	NN
cord-014597-66vd2mdu	1453	2	and	and	CC
cord-014597-66vd2mdu	1453	3	generation	generation	NN
cord-014597-66vd2mdu	1453	4	of	of	IN
cord-014597-66vd2mdu	1453	5	stable	stable	JJ
cord-014597-66vd2mdu	1453	6	pools	pool	NNS
cord-014597-66vd2mdu	1453	7	and	and	CC
cord-014597-66vd2mdu	1453	8	single	single	JJ
cord-014597-66vd2mdu	1453	9	cell	cell	NN
cord-014597-66vd2mdu	1453	10	clones	clone	NNS
cord-014597-66vd2mdu	1453	11	from	from	IN
cord-014597-66vd2mdu	1453	12	transfections	transfection	NNS
cord-014597-66vd2mdu	1453	13	with	with	IN
cord-014597-66vd2mdu	1453	14	IgG1-encoding	igg1-encoding	NN
cord-014597-66vd2mdu	1453	15	vectors	vector	NNS
cord-014597-66vd2mdu	1453	16	was	be	VBD
cord-014597-66vd2mdu	1453	17	performed	perform	VBN
cord-014597-66vd2mdu	1453	18	as	as	IN
cord-014597-66vd2mdu	1453	19	described	describe	VBN
cord-014597-66vd2mdu	1453	20	in	in	IN
cord-014597-66vd2mdu	1453	21	the	the	DT
cord-014597-66vd2mdu	1453	22	CHOZN	CHOZN	NNP
cord-014597-66vd2mdu	1453	23	®	®	NNP
cord-014597-66vd2mdu	1453	24	Platform	Platform	NNP
cord-014597-66vd2mdu	1453	25	Technical	Technical	NNP
cord-014597-66vd2mdu	1453	26	Bulletin	Bulletin	NNP
cord-014597-66vd2mdu	1453	27	.	.	.
cord-014597-66vd2mdu	1454	1	Titer	titer	NN
cord-014597-66vd2mdu	1454	2	analysis	analysis	NN
cord-014597-66vd2mdu	1454	3	was	be	VBD
cord-014597-66vd2mdu	1454	4	performed	perform	VBN
cord-014597-66vd2mdu	1454	5	in	in	IN
cord-014597-66vd2mdu	1454	6	static	static	JJ
cord-014597-66vd2mdu	1454	7	(	(	-LRB-
cord-014597-66vd2mdu	1454	8	96	96	CD
cord-014597-66vd2mdu	1454	9	well	well	NN
cord-014597-66vd2mdu	1454	10	plate	plate	NN
cord-014597-66vd2mdu	1454	11	)	)	-RRB-
cord-014597-66vd2mdu	1454	12	,	,	,
cord-014597-66vd2mdu	1454	13	in	in	IN
cord-014597-66vd2mdu	1454	14	a	a	DT
cord-014597-66vd2mdu	1454	15	7	7	CD
cord-014597-66vd2mdu	1454	16	day	day	NN
cord-014597-66vd2mdu	1454	17	TPP	tpp	NN
cord-014597-66vd2mdu	1454	18	assay	assay	NN
cord-014597-66vd2mdu	1454	19	and	and	CC
cord-014597-66vd2mdu	1454	20	in	in	IN
cord-014597-66vd2mdu	1454	21	a	a	DT
cord-014597-66vd2mdu	1454	22	14	14	CD
cord-014597-66vd2mdu	1454	23	day	day	NN
cord-014597-66vd2mdu	1454	24	fed	feed	VBN
cord-014597-66vd2mdu	1454	25	batch	batch	NN
cord-014597-66vd2mdu	1454	26	assay	assay	NN
cord-014597-66vd2mdu	1454	27	using	use	VBG
cord-014597-66vd2mdu	1454	28	a	a	DT
cord-014597-66vd2mdu	1454	29	QK	QK	NNP
cord-014597-66vd2mdu	1454	30	ForteBio	ForteBio	NNP
cord-014597-66vd2mdu	1454	31	.	.	.
cord-014597-66vd2mdu	1455	1	Initial	initial	JJ
cord-014597-66vd2mdu	1455	2	screening	screening	NN
cord-014597-66vd2mdu	1455	3	experiments	experiment	NNS
cord-014597-66vd2mdu	1455	4	identified	identify	VBD
cord-014597-66vd2mdu	1455	5	a	a	DT
cord-014597-66vd2mdu	1455	6	lead	lead	JJ
cord-014597-66vd2mdu	1455	7	vector	vector	NN
cord-014597-66vd2mdu	1455	8	,	,	,
cord-014597-66vd2mdu	1455	9	#	#	$
cord-014597-66vd2mdu	1455	10	39	39	CD
cord-014597-66vd2mdu	1455	11	,	,	,
cord-014597-66vd2mdu	1455	12	and	and	CC
cord-014597-66vd2mdu	1455	13	a	a	DT
cord-014597-66vd2mdu	1455	14	vector	vector	NN
cord-014597-66vd2mdu	1455	15	,	,	,
cord-014597-66vd2mdu	1455	16	#	#	$
cord-014597-66vd2mdu	1455	17	37	37	CD
cord-014597-66vd2mdu	1455	18	,	,	,
cord-014597-66vd2mdu	1455	19	which	which	WDT
cord-014597-66vd2mdu	1455	20	produced	produce	VBD
cord-014597-66vd2mdu	1455	21	very	very	RB
cord-014597-66vd2mdu	1455	22	low	low	JJ
cord-014597-66vd2mdu	1455	23	titers	titer	NNS
cord-014597-66vd2mdu	1455	24	and	and	CC
cord-014597-66vd2mdu	1455	25	relatively	relatively	RB
cord-014597-66vd2mdu	1455	26	few	few	JJ
cord-014597-66vd2mdu	1455	27	minipools	minipool	NNS
cord-014597-66vd2mdu	1455	28	expressing	express	VBG
cord-014597-66vd2mdu	1455	29	detectable	detectable	JJ
cord-014597-66vd2mdu	1455	30	levels	level	NNS
cord-014597-66vd2mdu	1455	31	of	of	IN
cord-014597-66vd2mdu	1455	32	IgG1	igg1	NN
cord-014597-66vd2mdu	1455	33	.	.	.
cord-014597-66vd2mdu	1456	1	Analysis	Analysis	NNP
cord-014597-66vd2mdu	1456	2	of	of	IN
cord-014597-66vd2mdu	1456	3	GFP	GFP	NNP
cord-014597-66vd2mdu	1456	4	and	and	CC
cord-014597-66vd2mdu	1456	5	RFP	RFP	NNP
cord-014597-66vd2mdu	1456	6	expression	expression	NN
cord-014597-66vd2mdu	1456	7	from	from	IN
cord-014597-66vd2mdu	1456	8	the	the	DT
cord-014597-66vd2mdu	1456	9	modified	modify	VBN
cord-014597-66vd2mdu	1456	10	vectors	vector	NNS
cord-014597-66vd2mdu	1456	11	indicated	indicate	VBD
cord-014597-66vd2mdu	1456	12	relatively	relatively	RB
cord-014597-66vd2mdu	1456	13	high	high	JJ
cord-014597-66vd2mdu	1456	14	expression	expression	NN
cord-014597-66vd2mdu	1456	15	from	from	IN
cord-014597-66vd2mdu	1456	16	the	the	DT
cord-014597-66vd2mdu	1456	17	RFP	RFP	NNP
cord-014597-66vd2mdu	1456	18	/	/	SYM
cord-014597-66vd2mdu	1456	19	HC	HC	NNP
cord-014597-66vd2mdu	1456	20	expression	expression	NN
cord-014597-66vd2mdu	1456	21	cassette	cassette	NN
cord-014597-66vd2mdu	1456	22	of	of	IN
cord-014597-66vd2mdu	1456	23	vector	vector	NN
cord-014597-66vd2mdu	1456	24	#	#	$
cord-014597-66vd2mdu	1456	25	37	37	CD
cord-014597-66vd2mdu	1456	26	.	.	.
cord-014597-66vd2mdu	1457	1	A	a	DT
cord-014597-66vd2mdu	1457	2	stronger	strong	JJR
cord-014597-66vd2mdu	1457	3	promoter	promoter	NN
cord-014597-66vd2mdu	1457	4	resulting	result	VBG
cord-014597-66vd2mdu	1457	5	in	in	IN
cord-014597-66vd2mdu	1457	6	overabundance	overabundance	NN
cord-014597-66vd2mdu	1457	7	of	of	IN
cord-014597-66vd2mdu	1457	8	HC	HC	NNP
cord-014597-66vd2mdu	1457	9	,	,	,
cord-014597-66vd2mdu	1457	10	known	know	VBN
cord-014597-66vd2mdu	1457	11	to	to	TO
cord-014597-66vd2mdu	1457	12	be	be	VB
cord-014597-66vd2mdu	1457	13	toxic	toxic	JJ
cord-014597-66vd2mdu	1457	14	to	to	IN
cord-014597-66vd2mdu	1457	15	cells	cell	NNS
cord-014597-66vd2mdu	1457	16	,	,	,
cord-014597-66vd2mdu	1457	17	provides	provide	VBZ
cord-014597-66vd2mdu	1457	18	a	a	DT
cord-014597-66vd2mdu	1457	19	possible	possible	JJ
cord-014597-66vd2mdu	1457	20	explanation	explanation	NN
cord-014597-66vd2mdu	1457	21	for	for	IN
cord-014597-66vd2mdu	1457	22	the	the	DT
cord-014597-66vd2mdu	1457	23	poor	poor	JJ
cord-014597-66vd2mdu	1457	24	results	result	NNS
cord-014597-66vd2mdu	1457	25	with	with	IN
cord-014597-66vd2mdu	1457	26	this	this	DT
cord-014597-66vd2mdu	1457	27	vector	vector	NN
cord-014597-66vd2mdu	1457	28	.	.	.
cord-014597-66vd2mdu	1458	1	Interestingly	interestingly	RB
cord-014597-66vd2mdu	1458	2	,	,	,
cord-014597-66vd2mdu	1458	3	swapping	swap	VBG
cord-014597-66vd2mdu	1458	4	the	the	DT
cord-014597-66vd2mdu	1458	5	positions	position	NNS
cord-014597-66vd2mdu	1458	6	of	of	IN
cord-014597-66vd2mdu	1458	7	the	the	DT
cord-014597-66vd2mdu	1458	8	LC	LC	NNP
cord-014597-66vd2mdu	1458	9	and	and	CC
cord-014597-66vd2mdu	1458	10	HC	HC	NNP
cord-014597-66vd2mdu	1458	11	in	in	IN
cord-014597-66vd2mdu	1458	12	#	#	$
cord-014597-66vd2mdu	1458	13	37	37	CD
cord-014597-66vd2mdu	1458	14	resulted	result	VBD
cord-014597-66vd2mdu	1458	15	in	in	IN
cord-014597-66vd2mdu	1458	16	a	a	DT
cord-014597-66vd2mdu	1458	17	vector	vector	NN
cord-014597-66vd2mdu	1458	18	,	,	,
cord-014597-66vd2mdu	1458	19	#	#	$
cord-014597-66vd2mdu	1458	20	77	77	CD
cord-014597-66vd2mdu	1458	21	,	,	,
cord-014597-66vd2mdu	1458	22	that	that	WDT
cord-014597-66vd2mdu	1458	23	outperformed	outperform	VBD
cord-014597-66vd2mdu	1458	24	the	the	DT
cord-014597-66vd2mdu	1458	25	initially	initially	RB
cord-014597-66vd2mdu	1458	26	identified	identify	VBN
cord-014597-66vd2mdu	1458	27	lead	lead	JJ
cord-014597-66vd2mdu	1458	28	vector	vector	NN
cord-014597-66vd2mdu	1458	29	(	(	-LRB-
cord-014597-66vd2mdu	1458	30	Fig	fig	NN
cord-014597-66vd2mdu	1458	31	.	.	.
cord-014597-66vd2mdu	1458	32	1	1	CD
cord-014597-66vd2mdu	1458	33	)	)	-RRB-
cord-014597-66vd2mdu	1458	34	.	.	.
cord-014597-66vd2mdu	1459	1	This	this	DT
cord-014597-66vd2mdu	1459	2	same	same	JJ
cord-014597-66vd2mdu	1459	3	change	change	NN
cord-014597-66vd2mdu	1459	4	was	be	VBD
cord-014597-66vd2mdu	1459	5	made	make	VBN
cord-014597-66vd2mdu	1459	6	to	to	TO
cord-014597-66vd2mdu	1459	7	vector	vector	VB
cord-014597-66vd2mdu	1459	8	#	#	$
cord-014597-66vd2mdu	1459	9	39	39	CD
cord-014597-66vd2mdu	1459	10	without	without	IN
cord-014597-66vd2mdu	1459	11	any	any	DT
cord-014597-66vd2mdu	1459	12	resulting	result	VBG
cord-014597-66vd2mdu	1459	13	improvement	improvement	NN
cord-014597-66vd2mdu	1459	14	in	in	IN
cord-014597-66vd2mdu	1459	15	titers	titer	NNS
cord-014597-66vd2mdu	1459	16	(	(	-LRB-
cord-014597-66vd2mdu	1459	17	vector	vector	NN
cord-014597-66vd2mdu	1459	18	#	#	$
cord-014597-66vd2mdu	1459	19	78	78	CD
cord-014597-66vd2mdu	1459	20	,	,	,
cord-014597-66vd2mdu	1459	21	Fig	Fig	NNP
cord-014597-66vd2mdu	1459	22	.	.	NNP
cord-014597-66vd2mdu	1459	23	1	1	CD
cord-014597-66vd2mdu	1459	24	)	)	-RRB-
cord-014597-66vd2mdu	1459	25	.	.	.
cord-014597-66vd2mdu	1460	1	Interestingly	interestingly	RB
cord-014597-66vd2mdu	1460	2	,	,	,
cord-014597-66vd2mdu	1460	3	vector	vector	NN
cord-014597-66vd2mdu	1460	4	#	#	$
cord-014597-66vd2mdu	1460	5	39	39	CD
cord-014597-66vd2mdu	1460	6	had	have	VBD
cord-014597-66vd2mdu	1460	7	a	a	DT
cord-014597-66vd2mdu	1460	8	smaller	small	JJR
cord-014597-66vd2mdu	1460	9	difference	difference	NN
cord-014597-66vd2mdu	1460	10	in	in	IN
cord-014597-66vd2mdu	1460	11	relative	relative	JJ
cord-014597-66vd2mdu	1460	12	promoter	promoter	NN
cord-014597-66vd2mdu	1460	13	strengths	strength	NNS
cord-014597-66vd2mdu	1460	14	,	,	,
cord-014597-66vd2mdu	1460	15	based	base	VBN
cord-014597-66vd2mdu	1460	16	on	on	IN
cord-014597-66vd2mdu	1460	17	mean	mean	JJ
cord-014597-66vd2mdu	1460	18	channel	channel	NN
cord-014597-66vd2mdu	1460	19	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	1460	20	ratio	ratio	NN
cord-014597-66vd2mdu	1460	21	of	of	IN
cord-014597-66vd2mdu	1460	22	GFP	GFP	NNP
cord-014597-66vd2mdu	1460	23	to	to	IN
cord-014597-66vd2mdu	1460	24	RFP	RFP	NNP
cord-014597-66vd2mdu	1460	25	,	,	,
cord-014597-66vd2mdu	1460	26	suggesting	suggest	VBG
cord-014597-66vd2mdu	1460	27	that	that	IN
cord-014597-66vd2mdu	1460	28	overabundance	overabundance	NN
cord-014597-66vd2mdu	1460	29	of	of	IN
cord-014597-66vd2mdu	1460	30	HC	HC	NNP
cord-014597-66vd2mdu	1460	31	was	be	VBD
cord-014597-66vd2mdu	1460	32	not	not	RB
cord-014597-66vd2mdu	1460	33	an	an	DT
cord-014597-66vd2mdu	1460	34	impediment	impediment	NN
cord-014597-66vd2mdu	1460	35	to	to	IN
cord-014597-66vd2mdu	1460	36	IgG1	igg1	CD
cord-014597-66vd2mdu	1460	37	expression	expression	NN
cord-014597-66vd2mdu	1460	38	from	from	IN
cord-014597-66vd2mdu	1460	39	#	#	$
cord-014597-66vd2mdu	1460	40	39	39	CD
cord-014597-66vd2mdu	1460	41	.	.	.
cord-014597-66vd2mdu	1461	1	Poor	poor	JJ
cord-014597-66vd2mdu	1461	2	titers	titer	NNS
cord-014597-66vd2mdu	1461	3	were	be	VBD
cord-014597-66vd2mdu	1461	4	also	also	RB
cord-014597-66vd2mdu	1461	5	seen	see	VBN
cord-014597-66vd2mdu	1461	6	with	with	IN
cord-014597-66vd2mdu	1461	7	a	a	DT
cord-014597-66vd2mdu	1461	8	modified	modify	VBN
cord-014597-66vd2mdu	1461	9	version	version	NN
cord-014597-66vd2mdu	1461	10	of	of	IN
cord-014597-66vd2mdu	1461	11	vector	vector	NN
cord-014597-66vd2mdu	1461	12	#	#	$
cord-014597-66vd2mdu	1461	13	39	39	CD
cord-014597-66vd2mdu	1461	14	(	(	-LRB-
cord-014597-66vd2mdu	1461	15	vector	vector	NN
cord-014597-66vd2mdu	1461	16	#	#	$
cord-014597-66vd2mdu	1461	17	79	79	CD
cord-014597-66vd2mdu	1461	18	,	,	,
cord-014597-66vd2mdu	1461	19	Fig	Fig	NNP
cord-014597-66vd2mdu	1461	20	.	.	NNP
cord-014597-66vd2mdu	1461	21	1	1	CD
cord-014597-66vd2mdu	1461	22	)	)	-RRB-
cord-014597-66vd2mdu	1461	23	in	in	IN
cord-014597-66vd2mdu	1461	24	which	which	WDT
cord-014597-66vd2mdu	1461	25	the	the	DT
cord-014597-66vd2mdu	1461	26	glutamine	glutamine	NN
cord-014597-66vd2mdu	1461	27	synthethase	synthethase	NN
cord-014597-66vd2mdu	1461	28	selection	selection	NN
cord-014597-66vd2mdu	1461	29	cassette	cassette	NN
cord-014597-66vd2mdu	1461	30	was	be	VBD
cord-014597-66vd2mdu	1461	31	in	in	IN
cord-014597-66vd2mdu	1461	32	the	the	DT
cord-014597-66vd2mdu	1461	33	reverse	reverse	JJ
cord-014597-66vd2mdu	1461	34	orientation	orientation	NN
cord-014597-66vd2mdu	1461	35	.	.	.
cord-014597-66vd2mdu	1462	1	This	this	DT
cord-014597-66vd2mdu	1462	2	second	second	JJ
cord-014597-66vd2mdu	1462	3	screen	screen	NN
cord-014597-66vd2mdu	1462	4	identified	identify	VBD
cord-014597-66vd2mdu	1462	5	vector	vector	NN
cord-014597-66vd2mdu	1462	6	#	#	$
cord-014597-66vd2mdu	1462	7	77	77	CD
cord-014597-66vd2mdu	1462	8	as	as	IN
cord-014597-66vd2mdu	1462	9	the	the	DT
cord-014597-66vd2mdu	1462	10	lead	lead	JJ
cord-014597-66vd2mdu	1462	11	vector	vector	NN
cord-014597-66vd2mdu	1462	12	design	design	NN
cord-014597-66vd2mdu	1462	13	(	(	-LRB-
cord-014597-66vd2mdu	1462	14	Fig	Fig	NNP
cord-014597-66vd2mdu	1462	15	.	.	.
cord-014597-66vd2mdu	1462	16	1	1	CD
cord-014597-66vd2mdu	1462	17	)	)	-RRB-
cord-014597-66vd2mdu	1462	18	.	.	.
cord-014597-66vd2mdu	1463	1	A	a	DT
cord-014597-66vd2mdu	1463	2	full	full	JJ
cord-014597-66vd2mdu	1463	3	comparative	comparative	JJ
cord-014597-66vd2mdu	1463	4	study	study	NN
cord-014597-66vd2mdu	1463	5	of	of	IN
cord-014597-66vd2mdu	1463	6	vector	vector	NN
cord-014597-66vd2mdu	1463	7	#	#	$
cord-014597-66vd2mdu	1463	8	77	77	CD
cord-014597-66vd2mdu	1463	9	and	and	CC
cord-014597-66vd2mdu	1463	10	the	the	DT
cord-014597-66vd2mdu	1463	11	control	control	NN
cord-014597-66vd2mdu	1463	12	vector	vector	NN
cord-014597-66vd2mdu	1463	13	was	be	VBD
cord-014597-66vd2mdu	1463	14	performed	perform	VBN
cord-014597-66vd2mdu	1463	15	,	,	,
cord-014597-66vd2mdu	1463	16	cumulating	cumulate	VBG
cord-014597-66vd2mdu	1463	17	in	in	IN
cord-014597-66vd2mdu	1463	18	the	the	DT
cord-014597-66vd2mdu	1463	19	generation	generation	NN
cord-014597-66vd2mdu	1463	20	and	and	CC
cord-014597-66vd2mdu	1463	21	comparison	comparison	NN
cord-014597-66vd2mdu	1463	22	of	of	IN
cord-014597-66vd2mdu	1463	23	single	single	JJ
cord-014597-66vd2mdu	1463	24	-	-	HYPH
cord-014597-66vd2mdu	1463	25	cell	cell	NN
cord-014597-66vd2mdu	1463	26	clones	clone	NNS
cord-014597-66vd2mdu	1463	27	from	from	IN
cord-014597-66vd2mdu	1463	28	each	each	DT
cord-014597-66vd2mdu	1463	29	.	.	.
cord-014597-66vd2mdu	1464	1	These	these	DT
cord-014597-66vd2mdu	1464	2	studies	study	NNS
cord-014597-66vd2mdu	1464	3	have	have	VBP
cord-014597-66vd2mdu	1464	4	demonstrated	demonstrate	VBN
cord-014597-66vd2mdu	1464	5	the	the	DT
cord-014597-66vd2mdu	1464	6	equivalence	equivalence	NN
cord-014597-66vd2mdu	1464	7	of	of	IN
cord-014597-66vd2mdu	1464	8	these	these	DT
cord-014597-66vd2mdu	1464	9	vectors	vector	NNS
cord-014597-66vd2mdu	1464	10	in	in	IN
cord-014597-66vd2mdu	1464	11	terms	term	NNS
cord-014597-66vd2mdu	1464	12	of	of	IN
cord-014597-66vd2mdu	1464	13	IgG1	IgG1	NNP
cord-014597-66vd2mdu	1464	14	titer	titer	NN
cord-014597-66vd2mdu	1464	15	.	.	.
cord-014597-66vd2mdu	1465	1	This	this	DT
cord-014597-66vd2mdu	1465	2	work	work	NN
cord-014597-66vd2mdu	1465	3	has	have	VBZ
cord-014597-66vd2mdu	1465	4	resulted	result	VBN
cord-014597-66vd2mdu	1465	5	in	in	IN
cord-014597-66vd2mdu	1465	6	the	the	DT
cord-014597-66vd2mdu	1465	7	identification	identification	NN
cord-014597-66vd2mdu	1465	8	and	and	CC
cord-014597-66vd2mdu	1465	9	characterization	characterization	NN
cord-014597-66vd2mdu	1465	10	of	of	IN
cord-014597-66vd2mdu	1465	11	a	a	DT
cord-014597-66vd2mdu	1465	12	dual	dual	JJ
cord-014597-66vd2mdu	1465	13	expression	expression	NN
cord-014597-66vd2mdu	1465	14	vector	vector	NN
cord-014597-66vd2mdu	1465	15	with	with	IN
cord-014597-66vd2mdu	1465	16	minimized	minimize	VBN
cord-014597-66vd2mdu	1465	17	similarity	similarity	NN
cord-014597-66vd2mdu	1465	18	between	between	IN
cord-014597-66vd2mdu	1465	19	the	the	DT
cord-014597-66vd2mdu	1465	20	two	two	CD
cord-014597-66vd2mdu	1465	21	expression	expression	NN
cord-014597-66vd2mdu	1465	22	cassettes	cassette	NNS
cord-014597-66vd2mdu	1465	23	,	,	,
cord-014597-66vd2mdu	1465	24	easing	ease	VBG
cord-014597-66vd2mdu	1465	25	the	the	DT
cord-014597-66vd2mdu	1465	26	cloning	cloning	NN
cord-014597-66vd2mdu	1465	27	,	,	,
cord-014597-66vd2mdu	1465	28	propagation	propagation	NN
cord-014597-66vd2mdu	1465	29	and	and	CC
cord-014597-66vd2mdu	1465	30	analysis	analysis	NN
cord-014597-66vd2mdu	1465	31	of	of	IN
cord-014597-66vd2mdu	1465	32	vector	vector	NN
cord-014597-66vd2mdu	1465	33	integration	integration	NN
cord-014597-66vd2mdu	1465	34	in	in	IN
cord-014597-66vd2mdu	1465	35	stable	stable	JJ
cord-014597-66vd2mdu	1465	36	cell	cell	NN
cord-014597-66vd2mdu	1465	37	lines	line	NNS
cord-014597-66vd2mdu	1465	38	while	while	IN
cord-014597-66vd2mdu	1465	39	maintaining	maintain	VBG
cord-014597-66vd2mdu	1465	40	the	the	DT
cord-014597-66vd2mdu	1465	41	high	high	JJ
cord-014597-66vd2mdu	1465	42	,	,	,
cord-014597-66vd2mdu	1465	43	stable	stable	JJ
cord-014597-66vd2mdu	1465	44	expression	expression	NN
cord-014597-66vd2mdu	1465	45	of	of	IN
cord-014597-66vd2mdu	1465	46	the	the	DT
cord-014597-66vd2mdu	1465	47	encoded	encode	VBN
cord-014597-66vd2mdu	1465	48	protein	protein	NN
cord-014597-66vd2mdu	1465	49	of	of	IN
cord-014597-66vd2mdu	1465	50	the	the	DT
cord-014597-66vd2mdu	1465	51	original	original	JJ
cord-014597-66vd2mdu	1465	52	vector	vector	NN
cord-014597-66vd2mdu	1465	53	design	design	NN
cord-014597-66vd2mdu	1465	54	.	.	.
cord-014597-66vd2mdu	1466	1	Background	background	VB
cord-014597-66vd2mdu	1466	2	Traditional	traditional	JJ
cord-014597-66vd2mdu	1466	3	cell	cell	NN
cord-014597-66vd2mdu	1466	4	line	line	NN
cord-014597-66vd2mdu	1466	5	engineering	engineering	NN
cord-014597-66vd2mdu	1466	6	strategies	strategy	NNS
cord-014597-66vd2mdu	1466	7	mainly	mainly	RB
cord-014597-66vd2mdu	1466	8	include	include	VBP
cord-014597-66vd2mdu	1466	9	an	an	DT
cord-014597-66vd2mdu	1466	10	antibiotics	antibiotic	NNS
cord-014597-66vd2mdu	1466	11	resistance	resistance	NN
cord-014597-66vd2mdu	1466	12	selection	selection	NN
cord-014597-66vd2mdu	1466	13	.	.	.
cord-014597-66vd2mdu	1467	1	In	in	IN
cord-014597-66vd2mdu	1467	2	this	this	DT
cord-014597-66vd2mdu	1467	3	process	process	NN
cord-014597-66vd2mdu	1467	4	,	,	,
cord-014597-66vd2mdu	1467	5	cells	cell	NNS
cord-014597-66vd2mdu	1467	6	are	be	VBP
cord-014597-66vd2mdu	1467	7	transfected	transfecte	VBN
cord-014597-66vd2mdu	1467	8	with	with	IN
cord-014597-66vd2mdu	1467	9	the	the	DT
cord-014597-66vd2mdu	1467	10	GoI	goi	NN
cord-014597-66vd2mdu	1467	11	(	(	-LRB-
cord-014597-66vd2mdu	1467	12	gene	gene	NN
cord-014597-66vd2mdu	1467	13	of	of	IN
cord-014597-66vd2mdu	1467	14	interest	interest	NN
cord-014597-66vd2mdu	1467	15	)	)	-RRB-
cord-014597-66vd2mdu	1467	16	together	together	RB
cord-014597-66vd2mdu	1467	17	with	with	IN
cord-014597-66vd2mdu	1467	18	an	an	DT
cord-014597-66vd2mdu	1467	19	antibiotics	antibiotic	NNS
cord-014597-66vd2mdu	1467	20	resistance	resistance	NN
cord-014597-66vd2mdu	1467	21	gene	gene	NN
cord-014597-66vd2mdu	1467	22	and	and	CC
cord-014597-66vd2mdu	1467	23	those	those	DT
cord-014597-66vd2mdu	1467	24	cells	cell	NNS
cord-014597-66vd2mdu	1467	25	are	be	VBP
cord-014597-66vd2mdu	1467	26	selected	select	VBN
cord-014597-66vd2mdu	1467	27	that	that	WDT
cord-014597-66vd2mdu	1467	28	survive	survive	VBP
cord-014597-66vd2mdu	1467	29	treatment	treatment	NN
cord-014597-66vd2mdu	1467	30	with	with	IN
cord-014597-66vd2mdu	1467	31	the	the	DT
cord-014597-66vd2mdu	1467	32	respective	respective	JJ
cord-014597-66vd2mdu	1467	33	antibiotic	antibiotic	NN
cord-014597-66vd2mdu	1467	34	[	[	-LRB-
cord-014597-66vd2mdu	1467	35	1	1	CD
cord-014597-66vd2mdu	1467	36	]	]	-RRB-
cord-014597-66vd2mdu	1467	37	.	.	.
cord-014597-66vd2mdu	1468	1	Although	although	IN
cord-014597-66vd2mdu	1468	2	the	the	DT
cord-014597-66vd2mdu	1468	3	gene	gene	NN
cord-014597-66vd2mdu	1468	4	responsible	responsible	JJ
cord-014597-66vd2mdu	1468	5	for	for	IN
cord-014597-66vd2mdu	1468	6	the	the	DT
cord-014597-66vd2mdu	1468	7	survival	survival	NN
cord-014597-66vd2mdu	1468	8	of	of	IN
cord-014597-66vd2mdu	1468	9	the	the	DT
cord-014597-66vd2mdu	1468	10	cell	cell	NN
cord-014597-66vd2mdu	1468	11	is	be	VBZ
cord-014597-66vd2mdu	1468	12	transfected	transfecte	VBN
cord-014597-66vd2mdu	1468	13	together	together	RB
cord-014597-66vd2mdu	1468	14	with	with	IN
cord-014597-66vd2mdu	1468	15	the	the	DT
cord-014597-66vd2mdu	1468	16	GoI	goi	NN
cord-014597-66vd2mdu	1468	17	,	,	,
cord-014597-66vd2mdu	1468	18	resistance	resistance	NN
cord-014597-66vd2mdu	1468	19	is	be	VBZ
cord-014597-66vd2mdu	1468	20	not	not	RB
cord-014597-66vd2mdu	1468	21	necessarily	necessarily	RB
cord-014597-66vd2mdu	1468	22	linked	link	VBN
cord-014597-66vd2mdu	1468	23	to	to	IN
cord-014597-66vd2mdu	1468	24	high	high	JJ
cord-014597-66vd2mdu	1468	25	GoI	goi	NN
cord-014597-66vd2mdu	1468	26	expression	expression	NN
cord-014597-66vd2mdu	1468	27	.	.	.
cord-014597-66vd2mdu	1469	1	Thus	thus	RB
cord-014597-66vd2mdu	1469	2	,	,	,
cord-014597-66vd2mdu	1469	3	a	a	DT
cord-014597-66vd2mdu	1469	4	significant	significant	JJ
cord-014597-66vd2mdu	1469	5	proportion	proportion	NN
cord-014597-66vd2mdu	1469	6	of	of	IN
cord-014597-66vd2mdu	1469	7	resistant	resistant	JJ
cord-014597-66vd2mdu	1469	8	cells	cell	NNS
cord-014597-66vd2mdu	1469	9	may	may	MD
cord-014597-66vd2mdu	1469	10	not	not	RB
cord-014597-66vd2mdu	1469	11	express	express	VB
cord-014597-66vd2mdu	1469	12	the	the	DT
cord-014597-66vd2mdu	1469	13	GoI	goi	NN
cord-014597-66vd2mdu	1469	14	at	at	RB
cord-014597-66vd2mdu	1469	15	all	all	RB
cord-014597-66vd2mdu	1469	16	,	,	,
cord-014597-66vd2mdu	1469	17	necessitating	necessitate	VBG
cord-014597-66vd2mdu	1469	18	the	the	DT
cord-014597-66vd2mdu	1469	19	search	search	NN
cord-014597-66vd2mdu	1469	20	for	for	IN
cord-014597-66vd2mdu	1469	21	alternative	alternative	NN
cord-014597-66vd2mdu	1469	22	,	,	,
cord-014597-66vd2mdu	1469	23	more	more	RBR
cord-014597-66vd2mdu	1469	24	closely	closely	RB
cord-014597-66vd2mdu	1469	25	linked	link	VBN
cord-014597-66vd2mdu	1469	26	selection	selection	NN
cord-014597-66vd2mdu	1469	27	systems	system	NNS
cord-014597-66vd2mdu	1469	28	.	.	.
cord-014597-66vd2mdu	1470	1	siRNAs	siRNAs	NNP
cord-014597-66vd2mdu	1471	1	(	(	-LRB-
cord-014597-66vd2mdu	1471	2	silencing	silence	VBG
cord-014597-66vd2mdu	1471	3	inducing	induce	VBG
cord-014597-66vd2mdu	1471	4	RNAs	rna	NNS
cord-014597-66vd2mdu	1471	5	)	)	-RRB-
cord-014597-66vd2mdu	1471	6	are	be	VBP
cord-014597-66vd2mdu	1471	7	short	short	JJ
cord-014597-66vd2mdu	1471	8	,	,	,
cord-014597-66vd2mdu	1471	9	noncoding	noncoding	JJ
cord-014597-66vd2mdu	1471	10	RNAs	rna	NNS
cord-014597-66vd2mdu	1471	11	that	that	WDT
cord-014597-66vd2mdu	1471	12	can	can	MD
cord-014597-66vd2mdu	1471	13	bind	bind	VB
cord-014597-66vd2mdu	1471	14	to	to	IN
cord-014597-66vd2mdu	1471	15	complementary	complementary	JJ
cord-014597-66vd2mdu	1471	16	mRNA	mrna	NN
cord-014597-66vd2mdu	1471	17	and	and	CC
cord-014597-66vd2mdu	1471	18	inhibit	inhibit	VB
cord-014597-66vd2mdu	1471	19	their	-PRON-	PRP$
cord-014597-66vd2mdu	1471	20	translation	translation	NN
cord-014597-66vd2mdu	1471	21	.	.	.
cord-014597-66vd2mdu	1472	1	This	this	DT
cord-014597-66vd2mdu	1472	2	function	function	NN
cord-014597-66vd2mdu	1472	3	has	have	VBZ
cord-014597-66vd2mdu	1472	4	been	be	VBN
cord-014597-66vd2mdu	1472	5	used	use	VBN
cord-014597-66vd2mdu	1472	6	in	in	IN
cord-014597-66vd2mdu	1472	7	many	many	JJ
cord-014597-66vd2mdu	1472	8	approaches	approach	NNS
cord-014597-66vd2mdu	1472	9	to	to	TO
cord-014597-66vd2mdu	1472	10	silence	silence	VB
cord-014597-66vd2mdu	1472	11	the	the	DT
cord-014597-66vd2mdu	1472	12	expression	expression	NN
cord-014597-66vd2mdu	1472	13	of	of	IN
cord-014597-66vd2mdu	1472	14	certain	certain	JJ
cord-014597-66vd2mdu	1472	15	genes	gene	NNS
cord-014597-66vd2mdu	1472	16	[	[	-LRB-
cord-014597-66vd2mdu	1472	17	2	2	CD
cord-014597-66vd2mdu	1472	18	]	]	-RRB-
cord-014597-66vd2mdu	1472	19	.	.	.
cord-014597-66vd2mdu	1473	1	With	with	IN
cord-014597-66vd2mdu	1473	2	their	-PRON-	PRP$
cord-014597-66vd2mdu	1473	3	short	short	JJ
cord-014597-66vd2mdu	1473	4	length	length	NN
cord-014597-66vd2mdu	1473	5	,	,	,
cord-014597-66vd2mdu	1473	6	siRNAs	siRNAs	NNP
cord-014597-66vd2mdu	1473	7	can	can	MD
cord-014597-66vd2mdu	1473	8	be	be	VB
cord-014597-66vd2mdu	1473	9	hidden	hide	VBN
cord-014597-66vd2mdu	1473	10	in	in	IN
cord-014597-66vd2mdu	1473	11	introns	intron	NNS
cord-014597-66vd2mdu	1473	12	(	(	-LRB-
cord-014597-66vd2mdu	1473	13	non	non	JJ
cord-014597-66vd2mdu	1473	14	-	-	JJ
cord-014597-66vd2mdu	1473	15	translating	translating	JJ
cord-014597-66vd2mdu	1473	16	regions	region	NNS
cord-014597-66vd2mdu	1473	17	)	)	-RRB-
cord-014597-66vd2mdu	1473	18	of	of	IN
cord-014597-66vd2mdu	1473	19	genes	gene	NNS
cord-014597-66vd2mdu	1473	20	,	,	,
cord-014597-66vd2mdu	1473	21	making	make	VBG
cord-014597-66vd2mdu	1473	22	it	-PRON-	PRP
cord-014597-66vd2mdu	1473	23	possible	possible	JJ
cord-014597-66vd2mdu	1473	24	to	to	TO
cord-014597-66vd2mdu	1473	25	couple	couple	VB
cord-014597-66vd2mdu	1473	26	the	the	DT
cord-014597-66vd2mdu	1473	27	expression	expression	NN
cord-014597-66vd2mdu	1473	28	of	of	IN
cord-014597-66vd2mdu	1473	29	a	a	DT
cord-014597-66vd2mdu	1473	30	siRNA	sirna	NN
cord-014597-66vd2mdu	1473	31	to	to	IN
cord-014597-66vd2mdu	1473	32	a	a	DT
cord-014597-66vd2mdu	1473	33	gene	gene	NN
cord-014597-66vd2mdu	1473	34	.	.	.
cord-014597-66vd2mdu	1474	1	This	this	DT
cord-014597-66vd2mdu	1474	2	way	way	NN
cord-014597-66vd2mdu	1474	3	a	a	DT
cord-014597-66vd2mdu	1474	4	cell	cell	NN
cord-014597-66vd2mdu	1474	5	produces	produce	VBZ
cord-014597-66vd2mdu	1474	6	a	a	DT
cord-014597-66vd2mdu	1474	7	correlating	correlate	VBG
cord-014597-66vd2mdu	1474	8	amount	amount	NN
cord-014597-66vd2mdu	1474	9	of	of	IN
cord-014597-66vd2mdu	1474	10	siRNA	siRNA	NNP
cord-014597-66vd2mdu	1474	11	when	when	WRB
cord-014597-66vd2mdu	1474	12	transcribing	transcribe	VBG
cord-014597-66vd2mdu	1474	13	the	the	DT
cord-014597-66vd2mdu	1474	14	gene	gene	NN
cord-014597-66vd2mdu	1474	15	,	,	,
cord-014597-66vd2mdu	1474	16	without	without	IN
cord-014597-66vd2mdu	1474	17	adding	add	VBG
cord-014597-66vd2mdu	1474	18	any	any	DT
cord-014597-66vd2mdu	1474	19	further	further	JJ
cord-014597-66vd2mdu	1474	20	translational	translational	JJ
cord-014597-66vd2mdu	1474	21	burden	burden	NN
cord-014597-66vd2mdu	1474	22	on	on	IN
cord-014597-66vd2mdu	1474	23	the	the	DT
cord-014597-66vd2mdu	1474	24	cell	cell	NN
cord-014597-66vd2mdu	1474	25	.	.	.
cord-014597-66vd2mdu	1475	1	The	the	DT
cord-014597-66vd2mdu	1475	2	co	co	NN
cord-014597-66vd2mdu	1475	3	-	-	NN
cord-014597-66vd2mdu	1475	4	expression	expression	NN
cord-014597-66vd2mdu	1475	5	of	of	IN
cord-014597-66vd2mdu	1475	6	the	the	DT
cord-014597-66vd2mdu	1475	7	siRNA	siRNA	NNP
cord-014597-66vd2mdu	1475	8	can	can	MD
cord-014597-66vd2mdu	1475	9	be	be	VB
cord-014597-66vd2mdu	1475	10	used	use	VBN
cord-014597-66vd2mdu	1475	11	as	as	IN
cord-014597-66vd2mdu	1475	12	a	a	DT
cord-014597-66vd2mdu	1475	13	selective	selective	JJ
cord-014597-66vd2mdu	1475	14	marker	marker	NN
cord-014597-66vd2mdu	1475	15	by	by	IN
cord-014597-66vd2mdu	1475	16	one	one	CD
cord-014597-66vd2mdu	1475	17	of	of	IN
cord-014597-66vd2mdu	1475	18	the	the	DT
cord-014597-66vd2mdu	1475	19	following	follow	VBG
cord-014597-66vd2mdu	1475	20	methods	method	NNS
cord-014597-66vd2mdu	1475	21	:	:	:
cord-014597-66vd2mdu	1475	22	(	(	-LRB-
cord-014597-66vd2mdu	1475	23	1	1	LS
cord-014597-66vd2mdu	1475	24	)	)	-RRB-
cord-014597-66vd2mdu	1475	25	Knock	knock	VB
cord-014597-66vd2mdu	1475	26	-	-	HYPH
cord-014597-66vd2mdu	1475	27	down	down	NN
cord-014597-66vd2mdu	1475	28	of	of	IN
cord-014597-66vd2mdu	1475	29	a	a	DT
cord-014597-66vd2mdu	1475	30	suicide	suicide	NN
cord-014597-66vd2mdu	1475	31	gene	gene	NN
cord-014597-66vd2mdu	1475	32	to	to	TO
cord-014597-66vd2mdu	1475	33	enable	enable	VB
cord-014597-66vd2mdu	1475	34	a	a	DT
cord-014597-66vd2mdu	1475	35	cell	cell	NN
cord-014597-66vd2mdu	1475	36	's	's	POS
cord-014597-66vd2mdu	1475	37	survival	survival	NN
cord-014597-66vd2mdu	1475	38	after	after	IN
cord-014597-66vd2mdu	1475	39	suicide	suicide	NN
cord-014597-66vd2mdu	1475	40	gene	gene	NN
cord-014597-66vd2mdu	1475	41	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1475	42	transfection	transfection	NN
cord-014597-66vd2mdu	1475	43	,	,	,
cord-014597-66vd2mdu	1475	44	(	(	-LRB-
cord-014597-66vd2mdu	1475	45	2	2	LS
cord-014597-66vd2mdu	1475	46	)	)	-RRB-
cord-014597-66vd2mdu	1475	47	down	down	IN
cord-014597-66vd2mdu	1475	48	-	-	HYPH
cord-014597-66vd2mdu	1475	49	regulation	regulation	NN
cord-014597-66vd2mdu	1475	50	of	of	IN
cord-014597-66vd2mdu	1475	51	a	a	DT
cord-014597-66vd2mdu	1475	52	surface	surface	NN
cord-014597-66vd2mdu	1475	53	marker	marker	NN
cord-014597-66vd2mdu	1475	54	which	which	WDT
cord-014597-66vd2mdu	1475	55	is	be	VBZ
cord-014597-66vd2mdu	1475	56	used	use	VBN
cord-014597-66vd2mdu	1475	57	in	in	IN
cord-014597-66vd2mdu	1475	58	MACS	mac	NNS
cord-014597-66vd2mdu	1475	59	(	(	-LRB-
cord-014597-66vd2mdu	1475	60	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1475	61	cell	cell	NN
cord-014597-66vd2mdu	1475	62	separation	separation	NN
cord-014597-66vd2mdu	1475	63	)	)	-RRB-
cord-014597-66vd2mdu	1475	64	to	to	TO
cord-014597-66vd2mdu	1475	65	filter	filter	VB
cord-014597-66vd2mdu	1475	66	out	out	RP
cord-014597-66vd2mdu	1475	67	wanted	want	VBN
cord-014597-66vd2mdu	1475	68	or	or	CC
cord-014597-66vd2mdu	1475	69	unwanted	unwanted	JJ
cord-014597-66vd2mdu	1475	70	cells	cell	NNS
cord-014597-66vd2mdu	1475	71	,	,	,
cord-014597-66vd2mdu	1475	72	and	and	CC
cord-014597-66vd2mdu	1475	73	(	(	-LRB-
cord-014597-66vd2mdu	1475	74	3	3	LS
cord-014597-66vd2mdu	1475	75	)	)	-RRB-
cord-014597-66vd2mdu	1475	76	inhibition	inhibition	NN
cord-014597-66vd2mdu	1475	77	of	of	IN
cord-014597-66vd2mdu	1475	78	a	a	DT
cord-014597-66vd2mdu	1475	79	fluorophore	fluorophore	NN
cord-014597-66vd2mdu	1475	80	marker	marker	NN
cord-014597-66vd2mdu	1475	81	for	for	IN
cord-014597-66vd2mdu	1475	82	selection	selection	NN
cord-014597-66vd2mdu	1475	83	using	use	VBG
cord-014597-66vd2mdu	1475	84	FACS	FACS	NNP
cord-014597-66vd2mdu	1475	85	without	without	IN
cord-014597-66vd2mdu	1475	86	product	product	NN
cord-014597-66vd2mdu	1475	87	specific	specific	JJ
cord-014597-66vd2mdu	1475	88	antibodies	antibody	NNS
cord-014597-66vd2mdu	1475	89	.	.	.
cord-014597-66vd2mdu	1476	1	For	for	IN
cord-014597-66vd2mdu	1476	2	siRNA	siRNA	NNP
cord-014597-66vd2mdu	1476	3	based	base	VBN
cord-014597-66vd2mdu	1476	4	cell	cell	NN
cord-014597-66vd2mdu	1476	5	selection	selection	NN
cord-014597-66vd2mdu	1476	6	systems	system	NNS
cord-014597-66vd2mdu	1476	7	,	,	,
cord-014597-66vd2mdu	1476	8	siRNAs	siRNAs	NNP
cord-014597-66vd2mdu	1476	9	replace	replace	VBP
cord-014597-66vd2mdu	1476	10	the	the	DT
cord-014597-66vd2mdu	1476	11	commonly	commonly	RB
cord-014597-66vd2mdu	1476	12	used	use	VBN
cord-014597-66vd2mdu	1476	13	antibiotics	antibiotic	NNS
cord-014597-66vd2mdu	1476	14	resistance	resistance	NN
cord-014597-66vd2mdu	1476	15	as	as	IN
cord-014597-66vd2mdu	1476	16	a	a	DT
cord-014597-66vd2mdu	1476	17	marker	marker	NN
cord-014597-66vd2mdu	1476	18	.	.	.
cord-014597-66vd2mdu	1477	1	Cells	cell	NNS
cord-014597-66vd2mdu	1477	2	that	that	WDT
cord-014597-66vd2mdu	1477	3	produce	produce	VBP
cord-014597-66vd2mdu	1477	4	GoI	goi	NN
cord-014597-66vd2mdu	1477	5	will	will	MD
cord-014597-66vd2mdu	1477	6	also	also	RB
cord-014597-66vd2mdu	1477	7	produce	produce	VB
cord-014597-66vd2mdu	1477	8	the	the	DT
cord-014597-66vd2mdu	1477	9	siRNA	sirna	NN
cord-014597-66vd2mdu	1477	10	that	that	WDT
cord-014597-66vd2mdu	1477	11	protects	protect	VBZ
cord-014597-66vd2mdu	1477	12	the	the	DT
cord-014597-66vd2mdu	1477	13	cell	cell	NN
cord-014597-66vd2mdu	1477	14	from	from	IN
cord-014597-66vd2mdu	1477	15	a	a	DT
cord-014597-66vd2mdu	1477	16	suicide	suicide	NN
cord-014597-66vd2mdu	1477	17	gene	gene	NN
cord-014597-66vd2mdu	1477	18	.	.	.
cord-014597-66vd2mdu	1478	1	The	the	DT
cord-014597-66vd2mdu	1478	2	selection	selection	NN
cord-014597-66vd2mdu	1478	3	protein	protein	NN
cord-014597-66vd2mdu	1478	4	(	(	-LRB-
cord-014597-66vd2mdu	1478	5	suicide	suicide	NN
cord-014597-66vd2mdu	1478	6	genes	gene	NNS
cord-014597-66vd2mdu	1478	7	,	,	,
cord-014597-66vd2mdu	1478	8	fluorophores	fluorophore	NNS
cord-014597-66vd2mdu	1478	9	,	,	,
cord-014597-66vd2mdu	1478	10	surface	surface	NN
cord-014597-66vd2mdu	1478	11	markers	marker	NNS
cord-014597-66vd2mdu	1478	12	,	,	,
cord-014597-66vd2mdu	1478	13	etc	etc	FW
cord-014597-66vd2mdu	1478	14	.	.	.
cord-014597-66vd2mdu	1478	15	)	)	-RRB-
cord-014597-66vd2mdu	1478	16	is	be	VBZ
cord-014597-66vd2mdu	1478	17	transfected	transfecte	VBN
cord-014597-66vd2mdu	1478	18	as	as	IN
cord-014597-66vd2mdu	1478	19	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1478	20	and	and	CC
cord-014597-66vd2mdu	1478	21	is	be	VBZ
cord-014597-66vd2mdu	1478	22	only	only	RB
cord-014597-66vd2mdu	1478	23	expressed	express	VBN
cord-014597-66vd2mdu	1478	24	during	during	IN
cord-014597-66vd2mdu	1478	25	selection	selection	NN
cord-014597-66vd2mdu	1478	26	.	.	.
cord-014597-66vd2mdu	1479	1	The	the	DT
cord-014597-66vd2mdu	1479	2	general	general	JJ
cord-014597-66vd2mdu	1479	3	process	process	NN
cord-014597-66vd2mdu	1479	4	is	be	VBZ
cord-014597-66vd2mdu	1479	5	outlined	outline	VBN
cord-014597-66vd2mdu	1479	6	in	in	IN
cord-014597-66vd2mdu	1479	7	Fig	Fig	NNP
cord-014597-66vd2mdu	1479	8	.	.	.
cord-014597-66vd2mdu	1480	1	1	1	LS
cord-014597-66vd2mdu	1480	2	.	.	.
cord-014597-66vd2mdu	1481	1	(	(	-LRB-
cord-014597-66vd2mdu	1481	2	A	a	NN
cord-014597-66vd2mdu	1481	3	)	)	-RRB-
cord-014597-66vd2mdu	1482	1	The	the	DT
cord-014597-66vd2mdu	1482	2	traditional	traditional	JJ
cord-014597-66vd2mdu	1482	3	antibiotics	antibiotic	NNS
cord-014597-66vd2mdu	1482	4	resistance	resistance	NN
cord-014597-66vd2mdu	1482	5	marker	marker	NN
cord-014597-66vd2mdu	1482	6	is	be	VBZ
cord-014597-66vd2mdu	1482	7	replaced	replace	VBN
cord-014597-66vd2mdu	1482	8	by	by	IN
cord-014597-66vd2mdu	1482	9	an	an	DT
cord-014597-66vd2mdu	1482	10	siRNA	siRNA	NNP
cord-014597-66vd2mdu	1482	11	,	,	,
cord-014597-66vd2mdu	1482	12	which	which	WDT
cord-014597-66vd2mdu	1482	13	is	be	VBZ
cord-014597-66vd2mdu	1482	14	cotranscribed	cotranscribe	VBN
cord-014597-66vd2mdu	1482	15	with	with	IN
cord-014597-66vd2mdu	1482	16	the	the	DT
cord-014597-66vd2mdu	1482	17	GoI.	GoI.	NNP
cord-014597-66vd2mdu	1483	1	Unlike	unlike	IN
cord-014597-66vd2mdu	1483	2	in	in	IN
cord-014597-66vd2mdu	1483	3	antibiotic	antibiotic	JJ
cord-014597-66vd2mdu	1483	4	resistance	resistance	NN
cord-014597-66vd2mdu	1483	5	,	,	,
cord-014597-66vd2mdu	1483	6	the	the	DT
cord-014597-66vd2mdu	1483	7	marker	marker	NN
cord-014597-66vd2mdu	1483	8	here	here	RB
cord-014597-66vd2mdu	1483	9	is	be	VBZ
cord-014597-66vd2mdu	1483	10	not	not	RB
cord-014597-66vd2mdu	1483	11	a	a	DT
cord-014597-66vd2mdu	1483	12	protein	protein	NN
cord-014597-66vd2mdu	1483	13	,	,	,
cord-014597-66vd2mdu	1483	14	reducing	reduce	VBG
cord-014597-66vd2mdu	1483	15	the	the	DT
cord-014597-66vd2mdu	1483	16	translational	translational	JJ
cord-014597-66vd2mdu	1483	17	burden	burden	NN
cord-014597-66vd2mdu	1483	18	and	and	CC
cord-014597-66vd2mdu	1483	19	providing	provide	VBG
cord-014597-66vd2mdu	1483	20	more	more	JJR
cord-014597-66vd2mdu	1483	21	resources	resource	NNS
cord-014597-66vd2mdu	1483	22	for	for	IN
cord-014597-66vd2mdu	1483	23	GoI	goi	NN
cord-014597-66vd2mdu	1483	24	production	production	NN
cord-014597-66vd2mdu	1483	25	[	[	-LRB-
cord-014597-66vd2mdu	1483	26	3	3	CD
cord-014597-66vd2mdu	1483	27	]	]	-RRB-
cord-014597-66vd2mdu	1483	28	.	.	.
cord-014597-66vd2mdu	1483	29	 	 	_SP
cord-014597-66vd2mdu	1484	1	Transfection	transfection	NN
cord-014597-66vd2mdu	1484	2	with	with	IN
cord-014597-66vd2mdu	1484	3	the	the	DT
cord-014597-66vd2mdu	1484	4	suicide	suicide	NN
cord-014597-66vd2mdu	1484	5	gene	gene	NN
cord-014597-66vd2mdu	1484	6	proved	prove	VBD
cord-014597-66vd2mdu	1484	7	to	to	TO
cord-014597-66vd2mdu	1484	8	be	be	VB
cord-014597-66vd2mdu	1484	9	100	100	CD
cord-014597-66vd2mdu	1484	10	%	%	NN
cord-014597-66vd2mdu	1484	11	lethal	lethal	JJ
cord-014597-66vd2mdu	1484	12	within	within	IN
cord-014597-66vd2mdu	1484	13	2	2	CD
cord-014597-66vd2mdu	1484	14	days	day	NNS
cord-014597-66vd2mdu	1484	15	,	,	,
cord-014597-66vd2mdu	1484	16	with	with	IN
cord-014597-66vd2mdu	1484	17	no	no	DT
cord-014597-66vd2mdu	1484	18	outgrowth	outgrowth	NN
cord-014597-66vd2mdu	1484	19	over	over	IN
cord-014597-66vd2mdu	1484	20	two	two	CD
cord-014597-66vd2mdu	1484	21	weeks	week	NNS
cord-014597-66vd2mdu	1484	22	.	.	.
cord-014597-66vd2mdu	1485	1	Protection	protection	NN
cord-014597-66vd2mdu	1485	2	by	by	IN
cord-014597-66vd2mdu	1485	3	expression	expression	NN
cord-014597-66vd2mdu	1485	4	of	of	IN
cord-014597-66vd2mdu	1485	5	the	the	DT
cord-014597-66vd2mdu	1485	6	siRNA	siRNA	NNP
cord-014597-66vd2mdu	1485	7	was	be	VBD
cord-014597-66vd2mdu	1485	8	shown	show	VBN
cord-014597-66vd2mdu	1485	9	to	to	TO
cord-014597-66vd2mdu	1485	10	be	be	VB
cord-014597-66vd2mdu	1485	11	efficient	efficient	JJ
cord-014597-66vd2mdu	1485	12	.	.	.
cord-014597-66vd2mdu	1486	1	Currently	currently	RB
cord-014597-66vd2mdu	1486	2	a	a	DT
cord-014597-66vd2mdu	1486	3	comparison	comparison	NN
cord-014597-66vd2mdu	1486	4	of	of	IN
cord-014597-66vd2mdu	1486	5	stable	stable	JJ
cord-014597-66vd2mdu	1486	6	cell	cell	NN
cord-014597-66vd2mdu	1486	7	line	line	NN
cord-014597-66vd2mdu	1486	8	development	development	NN
cord-014597-66vd2mdu	1486	9	programs	program	NNS
cord-014597-66vd2mdu	1486	10	based	base	VBN
cord-014597-66vd2mdu	1486	11	on	on	IN
cord-014597-66vd2mdu	1486	12	siRNA	siRNA	NNP
cord-014597-66vd2mdu	1486	13	selection	selection	NN
cord-014597-66vd2mdu	1486	14	and	and	CC
cord-014597-66vd2mdu	1486	15	neomycin	neomycin	NN
cord-014597-66vd2mdu	1486	16	selection	selection	NN
cord-014597-66vd2mdu	1486	17	is	be	VBZ
cord-014597-66vd2mdu	1486	18	ongoing	ongoing	JJ
cord-014597-66vd2mdu	1486	19	.	.	.
cord-014597-66vd2mdu	1487	1	Conclusions	conclusion	NNS
cord-014597-66vd2mdu	1488	1	The	the	DT
cord-014597-66vd2mdu	1488	2	novel	novel	NN
cord-014597-66vd2mdu	1488	3	selection	selection	NN
cord-014597-66vd2mdu	1488	4	system	system	NN
cord-014597-66vd2mdu	1488	5	should	should	MD
cord-014597-66vd2mdu	1488	6	speed	speed	VB
cord-014597-66vd2mdu	1488	7	up	up	RP
cord-014597-66vd2mdu	1488	8	cell	cell	NN
cord-014597-66vd2mdu	1488	9	line	line	NN
cord-014597-66vd2mdu	1488	10	development	development	NN
cord-014597-66vd2mdu	1488	11	,	,	,
cord-014597-66vd2mdu	1488	12	as	as	IN
cord-014597-66vd2mdu	1488	13	the	the	DT
cord-014597-66vd2mdu	1488	14	system	system	NN
cord-014597-66vd2mdu	1488	15	kills	kill	VBZ
cord-014597-66vd2mdu	1488	16	rapidly	rapidly	RB
cord-014597-66vd2mdu	1488	17	and	and	CC
cord-014597-66vd2mdu	1488	18	directly	directly	RB
cord-014597-66vd2mdu	1488	19	selects	select	VBZ
cord-014597-66vd2mdu	1488	20	for	for	IN
cord-014597-66vd2mdu	1488	21	cells	cell	NNS
cord-014597-66vd2mdu	1488	22	transcribing	transcribe	VBG
cord-014597-66vd2mdu	1488	23	the	the	DT
cord-014597-66vd2mdu	1488	24	product	product	NN
cord-014597-66vd2mdu	1488	25	gene	gene	NN
cord-014597-66vd2mdu	1488	26	on	on	IN
cord-014597-66vd2mdu	1488	27	a	a	DT
cord-014597-66vd2mdu	1488	28	high	high	JJ
cord-014597-66vd2mdu	1488	29	level	level	NN
cord-014597-66vd2mdu	1488	30	.	.	.
cord-014597-66vd2mdu	1489	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1489	2	expect	expect	VBP
cord-014597-66vd2mdu	1489	3	to	to	TO
cord-014597-66vd2mdu	1489	4	see	see	VB
cord-014597-66vd2mdu	1489	5	more	more	JJR
cord-014597-66vd2mdu	1489	6	high	high	JJ
cord-014597-66vd2mdu	1489	7	producers	producer	NNS
cord-014597-66vd2mdu	1489	8	earlier	early	RBR
cord-014597-66vd2mdu	1489	9	in	in	IN
cord-014597-66vd2mdu	1489	10	the	the	DT
cord-014597-66vd2mdu	1489	11	process	process	NN
cord-014597-66vd2mdu	1489	12	,	,	,
cord-014597-66vd2mdu	1489	13	which	which	WDT
cord-014597-66vd2mdu	1489	14	will	will	MD
cord-014597-66vd2mdu	1489	15	allow	allow	VB
cord-014597-66vd2mdu	1489	16	for	for	IN
cord-014597-66vd2mdu	1489	17	an	an	DT
cord-014597-66vd2mdu	1489	18	easier	easy	JJR
cord-014597-66vd2mdu	1489	19	and	and	CC
cord-014597-66vd2mdu	1489	20	faster	fast	JJR
cord-014597-66vd2mdu	1489	21	selection	selection	NN
cord-014597-66vd2mdu	1489	22	in	in	IN
cord-014597-66vd2mdu	1489	23	the	the	DT
cord-014597-66vd2mdu	1489	24	following	follow	VBG
cord-014597-66vd2mdu	1489	25	steps	step	NNS
cord-014597-66vd2mdu	1489	26	.	.	.
cord-014597-66vd2mdu	1490	1	siRNA	siRNA	NNP
cord-014597-66vd2mdu	1490	2	based	base	VBN
cord-014597-66vd2mdu	1490	3	selection	selection	NN
cord-014597-66vd2mdu	1490	4	offers	offer	VBZ
cord-014597-66vd2mdu	1490	5	great	great	JJ
cord-014597-66vd2mdu	1490	6	opportunities	opportunity	NNS
cord-014597-66vd2mdu	1490	7	.	.	.
cord-014597-66vd2mdu	1491	1	By	by	IN
cord-014597-66vd2mdu	1491	2	directly	directly	RB
cord-014597-66vd2mdu	1491	3	selecting	select	VBG
cord-014597-66vd2mdu	1491	4	based	base	VBN
cord-014597-66vd2mdu	1491	5	on	on	IN
cord-014597-66vd2mdu	1491	6	GoI	GoI	NNP
cord-014597-66vd2mdu	1491	7	transcription	transcription	NN
cord-014597-66vd2mdu	1491	8	and	and	CC
cord-014597-66vd2mdu	1491	9	not	not	RB
cord-014597-66vd2mdu	1491	10	a	a	DT
cord-014597-66vd2mdu	1491	11	proxy	proxy	JJ
cord-014597-66vd2mdu	1491	12	marker	marker	NN
cord-014597-66vd2mdu	1491	13	,	,	,
cord-014597-66vd2mdu	1491	14	we	-PRON-	PRP
cord-014597-66vd2mdu	1491	15	expect	expect	VBP
cord-014597-66vd2mdu	1491	16	more	more	RBR
cord-014597-66vd2mdu	1491	17	relevant	relevant	JJ
cord-014597-66vd2mdu	1491	18	cells	cell	NNS
cord-014597-66vd2mdu	1491	19	on	on	IN
cord-014597-66vd2mdu	1491	20	a	a	DT
cord-014597-66vd2mdu	1491	21	pool	pool	NN
cord-014597-66vd2mdu	1491	22	level	level	NN
cord-014597-66vd2mdu	1491	23	.	.	.
cord-014597-66vd2mdu	1492	1	In	in	IN
cord-014597-66vd2mdu	1492	2	addition	addition	NN
cord-014597-66vd2mdu	1492	3	,	,	,
cord-014597-66vd2mdu	1492	4	the	the	DT
cord-014597-66vd2mdu	1492	5	elimination	elimination	NN
cord-014597-66vd2mdu	1492	6	of	of	IN
cord-014597-66vd2mdu	1492	7	an	an	DT
cord-014597-66vd2mdu	1492	8	antibiotics	antibiotic	NNS
cord-014597-66vd2mdu	1492	9	resistance	resistance	NN
cord-014597-66vd2mdu	1492	10	allows	allow	VBZ
cord-014597-66vd2mdu	1492	11	more	more	JJR
cord-014597-66vd2mdu	1492	12	cellular	cellular	JJ
cord-014597-66vd2mdu	1492	13	resources	resource	NNS
cord-014597-66vd2mdu	1492	14	for	for	IN
cord-014597-66vd2mdu	1492	15	GoI	GoI	NNP
cord-014597-66vd2mdu	1492	16	production	production	NN
cord-014597-66vd2mdu	1492	17	.	.	.
cord-014597-66vd2mdu	1493	1	The	the	DT
cord-014597-66vd2mdu	1493	2	system	system	NN
cord-014597-66vd2mdu	1493	3	offers	offer	VBZ
cord-014597-66vd2mdu	1493	4	multiple	multiple	JJ
cord-014597-66vd2mdu	1493	5	ways	way	NNS
cord-014597-66vd2mdu	1493	6	of	of	IN
cord-014597-66vd2mdu	1493	7	application	application	NN
cord-014597-66vd2mdu	1493	8	,	,	,
cord-014597-66vd2mdu	1493	9	either	either	CC
cord-014597-66vd2mdu	1493	10	by	by	IN
cord-014597-66vd2mdu	1493	11	enriching	enrich	VBG
cord-014597-66vd2mdu	1493	12	wanted	want	VBD
cord-014597-66vd2mdu	1493	13	,	,	,
cord-014597-66vd2mdu	1493	14	or	or	CC
cord-014597-66vd2mdu	1493	15	depleting	deplete	VBG
cord-014597-66vd2mdu	1493	16	unwanted	unwanted	JJ
cord-014597-66vd2mdu	1493	17	cells	cell	NNS
cord-014597-66vd2mdu	1493	18	.	.	.
cord-014597-66vd2mdu	1494	1	Background	Background	NNP
cord-014597-66vd2mdu	1494	2	Single	single	JJ
cord-014597-66vd2mdu	1494	3	-	-	HYPH
cord-014597-66vd2mdu	1494	4	cell	cell	NN
cord-014597-66vd2mdu	1494	5	cloning	cloning	NN
cord-014597-66vd2mdu	1494	6	is	be	VBZ
cord-014597-66vd2mdu	1494	7	an	an	DT
cord-014597-66vd2mdu	1494	8	essential	essential	JJ
cord-014597-66vd2mdu	1494	9	step	step	NN
cord-014597-66vd2mdu	1494	10	used	use	VBN
cord-014597-66vd2mdu	1494	11	in	in	IN
cord-014597-66vd2mdu	1494	12	the	the	DT
cord-014597-66vd2mdu	1494	13	upstream	upstream	JJ
cord-014597-66vd2mdu	1494	14	development	development	NN
cord-014597-66vd2mdu	1494	15	of	of	IN
cord-014597-66vd2mdu	1494	16	transformed	transform	VBN
cord-014597-66vd2mdu	1494	17	cell	cell	NN
cord-014597-66vd2mdu	1494	18	lines	line	NNS
cord-014597-66vd2mdu	1494	19	for	for	IN
cord-014597-66vd2mdu	1494	20	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1494	21	protein	protein	NN
cord-014597-66vd2mdu	1494	22	production	production	NN
cord-014597-66vd2mdu	1494	23	.	.	.
cord-014597-66vd2mdu	1495	1	While	while	IN
cord-014597-66vd2mdu	1495	2	single	single	JJ
cord-014597-66vd2mdu	1495	3	-	-	HYPH
cord-014597-66vd2mdu	1495	4	cell	cell	NN
cord-014597-66vd2mdu	1495	5	clones	clone	NNS
cord-014597-66vd2mdu	1495	6	are	be	VBP
cord-014597-66vd2mdu	1495	7	typically	typically	RB
cord-014597-66vd2mdu	1495	8	used	use	VBN
cord-014597-66vd2mdu	1495	9	to	to	TO
cord-014597-66vd2mdu	1495	10	ensure	ensure	VB
cord-014597-66vd2mdu	1495	11	product	product	NN
cord-014597-66vd2mdu	1495	12	consistency	consistency	NN
cord-014597-66vd2mdu	1495	13	,	,	,
cord-014597-66vd2mdu	1495	14	such	such	JJ
cord-014597-66vd2mdu	1495	15	low	low	JJ
cord-014597-66vd2mdu	1495	16	cell	cell	NN
cord-014597-66vd2mdu	1495	17	density	density	NN
cord-014597-66vd2mdu	1495	18	cultures	culture	NNS
cord-014597-66vd2mdu	1495	19	present	present	VBP
cord-014597-66vd2mdu	1495	20	a	a	DT
cord-014597-66vd2mdu	1495	21	survival	survival	NN
cord-014597-66vd2mdu	1495	22	challenge	challenge	NN
cord-014597-66vd2mdu	1495	23	;	;	:
cord-014597-66vd2mdu	1495	24	cells	cell	NNS
cord-014597-66vd2mdu	1495	25	grow	grow	VBP
cord-014597-66vd2mdu	1495	26	more	more	RBR
cord-014597-66vd2mdu	1495	27	slowly	slowly	RB
cord-014597-66vd2mdu	1495	28	or	or	CC
cord-014597-66vd2mdu	1495	29	may	may	MD
cord-014597-66vd2mdu	1495	30	even	even	RB
cord-014597-66vd2mdu	1495	31	not	not	RB
cord-014597-66vd2mdu	1495	32	survive	survive	VB
cord-014597-66vd2mdu	1495	33	at	at	IN
cord-014597-66vd2mdu	1495	34	low	low	JJ
cord-014597-66vd2mdu	1495	35	densities	density	NNS
cord-014597-66vd2mdu	1495	36	in	in	IN
cord-014597-66vd2mdu	1495	37	protein	protein	NN
cord-014597-66vd2mdu	1495	38	-	-	HYPH
cord-014597-66vd2mdu	1495	39	free	free	JJ
cord-014597-66vd2mdu	1495	40	media	medium	NNS
cord-014597-66vd2mdu	1495	41	,	,	,
cord-014597-66vd2mdu	1495	42	costing	cost	VBG
cord-014597-66vd2mdu	1495	43	the	the	DT
cord-014597-66vd2mdu	1495	44	industry	industry	NN
cord-014597-66vd2mdu	1495	45	time	time	NN
cord-014597-66vd2mdu	1495	46	and	and	CC
cord-014597-66vd2mdu	1495	47	money	money	NN
cord-014597-66vd2mdu	1495	48	and	and	CC
cord-014597-66vd2mdu	1495	49	limiting	limit	VBG
cord-014597-66vd2mdu	1495	50	the	the	DT
cord-014597-66vd2mdu	1495	51	pool	pool	NN
cord-014597-66vd2mdu	1495	52	of	of	IN
cord-014597-66vd2mdu	1495	53	candidate	candidate	NN
cord-014597-66vd2mdu	1495	54	colonies	colony	NNS
cord-014597-66vd2mdu	1495	55	for	for	IN
cord-014597-66vd2mdu	1495	56	choice	choice	NN
cord-014597-66vd2mdu	1495	57	of	of	IN
cord-014597-66vd2mdu	1495	58	production	production	NN
cord-014597-66vd2mdu	1495	59	clones	clone	NNS
cord-014597-66vd2mdu	1495	60	[	[	-LRB-
cord-014597-66vd2mdu	1495	61	1	1	CD
cord-014597-66vd2mdu	1495	62	,	,	,
cord-014597-66vd2mdu	1495	63	2	2	CD
cord-014597-66vd2mdu	1495	64	]	]	-RRB-
cord-014597-66vd2mdu	1495	65	.	.	.
cord-014597-66vd2mdu	1496	1	To	to	TO
cord-014597-66vd2mdu	1496	2	address	address	VB
cord-014597-66vd2mdu	1496	3	this	this	DT
cord-014597-66vd2mdu	1496	4	problem	problem	NN
cord-014597-66vd2mdu	1496	5	,	,	,
cord-014597-66vd2mdu	1496	6	we	-PRON-	PRP
cord-014597-66vd2mdu	1496	7	aimed	aim	VBD
cord-014597-66vd2mdu	1496	8	to	to	TO
cord-014597-66vd2mdu	1496	9	develop	develop	VB
cord-014597-66vd2mdu	1496	10	a	a	DT
cord-014597-66vd2mdu	1496	11	highly	highly	RB
cord-014597-66vd2mdu	1496	12	efficient	efficient	JJ
cord-014597-66vd2mdu	1496	13	serum	serum	NN
cord-014597-66vd2mdu	1496	14	-	-	HYPH
cord-014597-66vd2mdu	1496	15	free	free	JJ
cord-014597-66vd2mdu	1496	16	medium	medium	NN
cord-014597-66vd2mdu	1496	17	suitable	suitable	JJ
cord-014597-66vd2mdu	1496	18	for	for	IN
cord-014597-66vd2mdu	1496	19	optimising	optimise	VBG
cord-014597-66vd2mdu	1496	20	single	single	JJ
cord-014597-66vd2mdu	1496	21	-	-	HYPH
cord-014597-66vd2mdu	1496	22	cell	cell	NN
cord-014597-66vd2mdu	1496	23	cloning	cloning	NN
cord-014597-66vd2mdu	1496	24	efficiency	efficiency	NN
cord-014597-66vd2mdu	1496	25	by	by	IN
cord-014597-66vd2mdu	1496	26	studying	study	VBG
cord-014597-66vd2mdu	1496	27	a	a	DT
cord-014597-66vd2mdu	1496	28	range	range	NN
cord-014597-66vd2mdu	1496	29	of	of	IN
cord-014597-66vd2mdu	1496	30	conditioned	condition	VBN
cord-014597-66vd2mdu	1496	31	media	medium	NNS
cord-014597-66vd2mdu	1496	32	(	(	-LRB-
cord-014597-66vd2mdu	1496	33	CM	CM	NNP
cord-014597-66vd2mdu	1496	34	)	)	-RRB-
cord-014597-66vd2mdu	1496	35	samples	sample	NNS
cord-014597-66vd2mdu	1496	36	isolated	isolate	VBN
cord-014597-66vd2mdu	1496	37	from	from	IN
cord-014597-66vd2mdu	1496	38	different	different	JJ
cord-014597-66vd2mdu	1496	39	Chinese	chinese	JJ
cord-014597-66vd2mdu	1496	40	Hamster	Hamster	NNP
cord-014597-66vd2mdu	1496	41	Ovary	Ovary	NNP
cord-014597-66vd2mdu	1496	42	(	(	-LRB-
cord-014597-66vd2mdu	1496	43	CHO	CHO	NNP
cord-014597-66vd2mdu	1496	44	)	)	-RRB-
cord-014597-66vd2mdu	1496	45	cell	cell	NN
cord-014597-66vd2mdu	1496	46	lines	line	NNS
cord-014597-66vd2mdu	1496	47	.	.	.
cord-014597-66vd2mdu	1497	1	Materials	material	NNS
cord-014597-66vd2mdu	1497	2	and	and	CC
cord-014597-66vd2mdu	1497	3	methods	method	NNS
cord-014597-66vd2mdu	1497	4	CHO	CHO	NNP
cord-014597-66vd2mdu	1497	5	-	-	HYPH
cord-014597-66vd2mdu	1497	6	S	S	NNP
cord-014597-66vd2mdu	1497	7	,	,	,
cord-014597-66vd2mdu	1497	8	DG44	DG44	NNP
cord-014597-66vd2mdu	1497	9	and	and	CC
cord-014597-66vd2mdu	1497	10	CHO	CHO	NNP
cord-014597-66vd2mdu	1497	11	-	-	HYPH
cord-014597-66vd2mdu	1497	12	K1	K1	NNP
cord-014597-66vd2mdu	1497	13	were	be	VBD
cord-014597-66vd2mdu	1497	14	adapted	adapt	VBN
cord-014597-66vd2mdu	1497	15	to	to	IN
cord-014597-66vd2mdu	1497	16	CHO	CHO	NNP
cord-014597-66vd2mdu	1497	17	-	-	HYPH
cord-014597-66vd2mdu	1497	18	S	S	NNP
cord-014597-66vd2mdu	1497	19	SFM	SFM	NNP
cord-014597-66vd2mdu	1497	20	-	-	HYPH
cord-014597-66vd2mdu	1497	21	II	II	NNP
cord-014597-66vd2mdu	1498	1	(	(	-LRB-
cord-014597-66vd2mdu	1498	2	Gibco	Gibco	NNP
cord-014597-66vd2mdu	1498	3	)	)	-RRB-
cord-014597-66vd2mdu	1498	4	medium	medium	NN
cord-014597-66vd2mdu	1498	5	for	for	IN
cord-014597-66vd2mdu	1498	6	a	a	DT
cord-014597-66vd2mdu	1498	7	minimum	minimum	NN
cord-014597-66vd2mdu	1498	8	of	of	IN
cord-014597-66vd2mdu	1498	9	three	three	CD
cord-014597-66vd2mdu	1498	10	passages	passage	NNS
cord-014597-66vd2mdu	1498	11	.	.	.
cord-014597-66vd2mdu	1499	1	Conditioned	conditioned	JJ
cord-014597-66vd2mdu	1499	2	media	medium	NNS
cord-014597-66vd2mdu	1499	3	was	be	VBD
cord-014597-66vd2mdu	1499	4	then	then	RB
cord-014597-66vd2mdu	1499	5	collected	collect	VBN
cord-014597-66vd2mdu	1499	6	when	when	WRB
cord-014597-66vd2mdu	1499	7	the	the	DT
cord-014597-66vd2mdu	1499	8	cultures	culture	NNS
cord-014597-66vd2mdu	1499	9	reached	reach	VBD
cord-014597-66vd2mdu	1499	10	a	a	DT
cord-014597-66vd2mdu	1499	11	cell	cell	NN
cord-014597-66vd2mdu	1499	12	density	density	NN
cord-014597-66vd2mdu	1499	13	of	of	IN
cord-014597-66vd2mdu	1499	14	1x10	1x10	CD
cord-014597-66vd2mdu	1499	15	6	6	CD
cord-014597-66vd2mdu	1499	16	cells	cell	NNS
cord-014597-66vd2mdu	1499	17	/	/	SYM
cord-014597-66vd2mdu	1499	18	ml	ml	NN
cord-014597-66vd2mdu	1499	19	(	(	-LRB-
cord-014597-66vd2mdu	1499	20	typically	typically	RB
cord-014597-66vd2mdu	1499	21	day	day	NN
cord-014597-66vd2mdu	1499	22	2-day	2-day	JJ
cord-014597-66vd2mdu	1499	23	3	3	CD
cord-014597-66vd2mdu	1499	24	depending	depend	VBG
cord-014597-66vd2mdu	1499	25	on	on	IN
cord-014597-66vd2mdu	1499	26	the	the	DT
cord-014597-66vd2mdu	1499	27	growth	growth	NN
cord-014597-66vd2mdu	1499	28	profiles	profile	NNS
cord-014597-66vd2mdu	1499	29	of	of	IN
cord-014597-66vd2mdu	1499	30	each	each	DT
cord-014597-66vd2mdu	1499	31	cell	cell	NN
cord-014597-66vd2mdu	1499	32	line	line	NN
cord-014597-66vd2mdu	1499	33	and	and	CC
cord-014597-66vd2mdu	1499	34	whether	whether	IN
cord-014597-66vd2mdu	1499	35	they	-PRON-	PRP
cord-014597-66vd2mdu	1499	36	grew	grow	VBD
cord-014597-66vd2mdu	1499	37	in	in	IN
cord-014597-66vd2mdu	1499	38	suspension	suspension	NN
cord-014597-66vd2mdu	1499	39	or	or	CC
cord-014597-66vd2mdu	1499	40	attached	attach	VBN
cord-014597-66vd2mdu	1499	41	conditions	condition	NNS
cord-014597-66vd2mdu	1499	42	)	)	-RRB-
cord-014597-66vd2mdu	1499	43	.	.	.
cord-014597-66vd2mdu	1500	1	Samples	sample	NNS
cord-014597-66vd2mdu	1500	2	were	be	VBD
cord-014597-66vd2mdu	1500	3	then	then	RB
cord-014597-66vd2mdu	1500	4	centrifuged	centrifuge	VBN
cord-014597-66vd2mdu	1500	5	twice	twice	RB
cord-014597-66vd2mdu	1500	6	to	to	TO
cord-014597-66vd2mdu	1500	7	remove	remove	VB
cord-014597-66vd2mdu	1500	8	cell	cell	NN
cord-014597-66vd2mdu	1500	9	pellet	pellet	NN
cord-014597-66vd2mdu	1500	10	/	/	SYM
cord-014597-66vd2mdu	1500	11	debris	debris	NN
cord-014597-66vd2mdu	1500	12	and	and	CC
cord-014597-66vd2mdu	1500	13	stored	store	VBN
cord-014597-66vd2mdu	1500	14	at	at	IN
cord-014597-66vd2mdu	1500	15	-20	-20	NNP
cord-014597-66vd2mdu	1500	16	°	°	NN
cord-014597-66vd2mdu	1500	17	C	C	NNP
cord-014597-66vd2mdu	1500	18	.	.	.
cord-014597-66vd2mdu	1501	1	The	the	DT
cord-014597-66vd2mdu	1501	2	ability	ability	NN
cord-014597-66vd2mdu	1501	3	of	of	IN
cord-014597-66vd2mdu	1501	4	conditioned	condition	VBN
cord-014597-66vd2mdu	1501	5	media	medium	NNS
cord-014597-66vd2mdu	1501	6	to	to	TO
cord-014597-66vd2mdu	1501	7	support	support	VB
cord-014597-66vd2mdu	1501	8	CHO	CHO	NNP
cord-014597-66vd2mdu	1501	9	colony	colony	NN
cord-014597-66vd2mdu	1501	10	formation	formation	NN
cord-014597-66vd2mdu	1501	11	was	be	VBD
cord-014597-66vd2mdu	1501	12	then	then	RB
cord-014597-66vd2mdu	1501	13	assayed	assay	VBN
cord-014597-66vd2mdu	1501	14	using	use	VBG
cord-014597-66vd2mdu	1501	15	96-well	96-well	CD
cord-014597-66vd2mdu	1501	16	plates	plate	NNS
cord-014597-66vd2mdu	1501	17	,	,	,
cord-014597-66vd2mdu	1501	18	seeding	seed	VBG
cord-014597-66vd2mdu	1501	19	the	the	DT
cord-014597-66vd2mdu	1501	20	cells	cell	NNS
cord-014597-66vd2mdu	1501	21	at	at	IN
cord-014597-66vd2mdu	1501	22	low	low	JJ
cord-014597-66vd2mdu	1501	23	cell	cell	NN
cord-014597-66vd2mdu	1501	24	density	density	NN
cord-014597-66vd2mdu	1501	25	(	(	-LRB-
cord-014597-66vd2mdu	1501	26	1	1	CD
cord-014597-66vd2mdu	1501	27	-	-	HYPH
cord-014597-66vd2mdu	1501	28	10cells	10cells	CD
cord-014597-66vd2mdu	1501	29	/	/	SYM
cord-014597-66vd2mdu	1501	30	well	well	RB
cord-014597-66vd2mdu	1501	31	)	)	-RRB-
cord-014597-66vd2mdu	1501	32	by	by	IN
cord-014597-66vd2mdu	1501	33	diluting	dilute	VBG
cord-014597-66vd2mdu	1501	34	down	down	RP
cord-014597-66vd2mdu	1501	35	CHO	CHO	NNP
cord-014597-66vd2mdu	1501	36	cultures	culture	NNS
cord-014597-66vd2mdu	1501	37	in	in	IN
cord-014597-66vd2mdu	1501	38	media	medium	NNS
cord-014597-66vd2mdu	1501	39	/	/	SYM
cord-014597-66vd2mdu	1501	40	conditioned	condition	VBN
cord-014597-66vd2mdu	1501	41	media	medium	NNS
cord-014597-66vd2mdu	1501	42	.	.	.
cord-014597-66vd2mdu	1502	1	After	after	IN
cord-014597-66vd2mdu	1502	2	incubation	incubation	NN
cord-014597-66vd2mdu	1502	3	at	at	IN
cord-014597-66vd2mdu	1502	4	37	37	CD
cord-014597-66vd2mdu	1502	5	°	°	NN
cord-014597-66vd2mdu	1502	6	C	c	NN
cord-014597-66vd2mdu	1502	7	for	for	IN
cord-014597-66vd2mdu	1502	8	10	10	CD
cord-014597-66vd2mdu	1502	9	days	day	NNS
cord-014597-66vd2mdu	1502	10	,	,	,
cord-014597-66vd2mdu	1502	11	cloning	clone	VBG
cord-014597-66vd2mdu	1502	12	efficiency	efficiency	NN
cord-014597-66vd2mdu	1502	13	was	be	VBD
cord-014597-66vd2mdu	1502	14	assayed	assay	VBN
cord-014597-66vd2mdu	1502	15	using	use	VBG
cord-014597-66vd2mdu	1502	16	a	a	DT
cord-014597-66vd2mdu	1502	17	standard	standard	JJ
cord-014597-66vd2mdu	1502	18	XTT	XTT	NNP
cord-014597-66vd2mdu	1502	19	assay	assay	NN
cord-014597-66vd2mdu	1502	20	.	.	.
cord-014597-66vd2mdu	1503	1	Initial	initial	JJ
cord-014597-66vd2mdu	1503	2	screening	screening	NN
cord-014597-66vd2mdu	1503	3	of	of	IN
cord-014597-66vd2mdu	1503	4	the	the	DT
cord-014597-66vd2mdu	1503	5	nine	nine	CD
cord-014597-66vd2mdu	1503	6	CM	cm	NN
cord-014597-66vd2mdu	1503	7	samples	sample	NNS
cord-014597-66vd2mdu	1503	8	was	be	VBD
cord-014597-66vd2mdu	1503	9	performed	perform	VBN
cord-014597-66vd2mdu	1503	10	using	use	VBG
cord-014597-66vd2mdu	1503	11	CHO	CHO	NNP
cord-014597-66vd2mdu	1503	12	-	-	HYPH
cord-014597-66vd2mdu	1503	13	K1	K1	NNP
cord-014597-66vd2mdu	1503	14	cells	cell	NNS
cord-014597-66vd2mdu	1503	15	due	due	JJ
cord-014597-66vd2mdu	1503	16	to	to	IN
cord-014597-66vd2mdu	1503	17	their	-PRON-	PRP$
cord-014597-66vd2mdu	1503	18	widespread	widespread	JJ
cord-014597-66vd2mdu	1503	19	use	use	NN
cord-014597-66vd2mdu	1503	20	in	in	IN
cord-014597-66vd2mdu	1503	21	industrial	industrial	JJ
cord-014597-66vd2mdu	1503	22	antibody	antibody	NN
cord-014597-66vd2mdu	1503	23	production	production	NN
cord-014597-66vd2mdu	1503	24	.	.	.
cord-014597-66vd2mdu	1504	1	Successful	successful	JJ
cord-014597-66vd2mdu	1504	2	media	medium	NNS
cord-014597-66vd2mdu	1504	3	candidates	candidate	NNS
cord-014597-66vd2mdu	1504	4	were	be	VBD
cord-014597-66vd2mdu	1504	5	subsequently	subsequently	RB
cord-014597-66vd2mdu	1504	6	screened	screen	VBN
cord-014597-66vd2mdu	1504	7	using	use	VBG
cord-014597-66vd2mdu	1504	8	additional	additional	JJ
cord-014597-66vd2mdu	1504	9	CHO	CHO	NNP
cord-014597-66vd2mdu	1504	10	cell	cell	NN
cord-014597-66vd2mdu	1504	11	lines	line	NNS
cord-014597-66vd2mdu	1504	12	.	.	.
cord-014597-66vd2mdu	1505	1	Table	table	NN
cord-014597-66vd2mdu	1505	2	1	1	CD
cord-014597-66vd2mdu	1505	3	)	)	-RRB-
cord-014597-66vd2mdu	1505	4	.	.	.
cord-014597-66vd2mdu	1506	1	The	the	DT
cord-014597-66vd2mdu	1506	2	K1-SFMII	K1-SFMII	NNP
cord-014597-66vd2mdu	1506	3	-	-	HYPH
cord-014597-66vd2mdu	1506	4	CM	CM	NNP
cord-014597-66vd2mdu	1506	5	product	product	NN
cord-014597-66vd2mdu	1506	6	improved	improve	VBN
cord-014597-66vd2mdu	1506	7	cell	cell	NN
cord-014597-66vd2mdu	1506	8	cloning	cloning	NN
cord-014597-66vd2mdu	1506	9	efficiency	efficiency	NN
cord-014597-66vd2mdu	1506	10	for	for	IN
cord-014597-66vd2mdu	1506	11	DG44	DG44	NNP
cord-014597-66vd2mdu	1506	12	cells	cell	NNS
cord-014597-66vd2mdu	1506	13	(	(	-LRB-
cord-014597-66vd2mdu	1506	14	avg	avg	NN
cord-014597-66vd2mdu	1506	15	.	.	.
cord-014597-66vd2mdu	1507	1	increase>1.5-fold	increase>1.5-fold	NNP
cord-014597-66vd2mdu	1507	2	)	)	-RRB-
cord-014597-66vd2mdu	1507	3	and	and	CC
cord-014597-66vd2mdu	1507	4	CHO	CHO	NNP
cord-014597-66vd2mdu	1507	5	-	-	HYPH
cord-014597-66vd2mdu	1507	6	S	S	NNP
cord-014597-66vd2mdu	1507	7	cells	cell	NNS
cord-014597-66vd2mdu	1507	8	(	(	-LRB-
cord-014597-66vd2mdu	1507	9	avg	avg	NNP
cord-014597-66vd2mdu	1507	10	.	.	NNP
cord-014597-66vd2mdu	1507	11	increase>3-fold	increase>3-fold	CD
cord-014597-66vd2mdu	1507	12	)	)	-RRB-
cord-014597-66vd2mdu	1508	1	(	(	-LRB-
cord-014597-66vd2mdu	1508	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1508	3	.	.	.
cord-014597-66vd2mdu	1509	1	1	1	LS
cord-014597-66vd2mdu	1509	2	)	)	-RRB-
cord-014597-66vd2mdu	1509	3	and	and	CC
cord-014597-66vd2mdu	1509	4	also	also	RB
cord-014597-66vd2mdu	1509	5	the	the	DT
cord-014597-66vd2mdu	1509	6	adherent	adherent	JJ
cord-014597-66vd2mdu	1509	7	CHO	CHO	NNP
cord-014597-66vd2mdu	1509	8	-	-	HYPH
cord-014597-66vd2mdu	1509	9	K1	K1	NNP
cord-014597-66vd2mdu	1509	10	cell	cell	NN
cord-014597-66vd2mdu	1509	11	line	line	NN
cord-014597-66vd2mdu	1509	12	growing	grow	VBG
cord-014597-66vd2mdu	1509	13	in	in	IN
cord-014597-66vd2mdu	1509	14	ATCC	ATCC	NNP
cord-014597-66vd2mdu	1509	15	+5%FBS	+5%fbs	NN
cord-014597-66vd2mdu	1509	16	.	.	.
cord-014597-66vd2mdu	1510	1	The	the	DT
cord-014597-66vd2mdu	1510	2	ability	ability	NN
cord-014597-66vd2mdu	1510	3	of	of	IN
cord-014597-66vd2mdu	1510	4	conditioned	condition	VBN
cord-014597-66vd2mdu	1510	5	media	medium	NNS
cord-014597-66vd2mdu	1510	6	to	to	TO
cord-014597-66vd2mdu	1510	7	support	support	VB
cord-014597-66vd2mdu	1510	8	CHO	CHO	NNP
cord-014597-66vd2mdu	1510	9	growth	growth	NN
cord-014597-66vd2mdu	1510	10	in	in	IN
cord-014597-66vd2mdu	1510	11	limiting	limit	VBG
cord-014597-66vd2mdu	1510	12	-	-	HYPH
cord-014597-66vd2mdu	1510	13	dilution	dilution	NN
cord-014597-66vd2mdu	1510	14	conditions	condition	NNS
cord-014597-66vd2mdu	1510	15	(	(	-LRB-
cord-014597-66vd2mdu	1510	16	1	1	CD
cord-014597-66vd2mdu	1510	17	,	,	,
cord-014597-66vd2mdu	1510	18	6	6	CD
cord-014597-66vd2mdu	1510	19	and	and	CC
cord-014597-66vd2mdu	1510	20	10	10	CD
cord-014597-66vd2mdu	1510	21	cells	cell	NNS
cord-014597-66vd2mdu	1510	22	/	/	SYM
cord-014597-66vd2mdu	1510	23	ml	ml	NN
cord-014597-66vd2mdu	1510	24	)	)	-RRB-
cord-014597-66vd2mdu	1510	25	was	be	VBD
cord-014597-66vd2mdu	1510	26	investigated	investigate	VBN
cord-014597-66vd2mdu	1510	27	.	.	.
cord-014597-66vd2mdu	1511	1	From	from	IN
cord-014597-66vd2mdu	1511	2	a	a	DT
cord-014597-66vd2mdu	1511	3	range	range	NN
cord-014597-66vd2mdu	1511	4	of	of	IN
cord-014597-66vd2mdu	1511	5	nine	nine	CD
cord-014597-66vd2mdu	1511	6	conditioned	condition	VBN
cord-014597-66vd2mdu	1511	7	media	medium	NNS
cord-014597-66vd2mdu	1511	8	samples	sample	NNS
cord-014597-66vd2mdu	1511	9	;	;	:
cord-014597-66vd2mdu	1511	10	four	four	CD
cord-014597-66vd2mdu	1511	11	compelling	compelling	JJ
cord-014597-66vd2mdu	1511	12	products	product	NNS
cord-014597-66vd2mdu	1511	13	have	have	VBP
cord-014597-66vd2mdu	1511	14	been	be	VBN
cord-014597-66vd2mdu	1511	15	identified	identify	VBN
cord-014597-66vd2mdu	1511	16	which	which	WDT
cord-014597-66vd2mdu	1511	17	improve	improve	VBP
cord-014597-66vd2mdu	1511	18	low	low	JJ
cord-014597-66vd2mdu	1511	19	-	-	HYPH
cord-014597-66vd2mdu	1511	20	cell	cell	NN
cord-014597-66vd2mdu	1511	21	density	density	NN
cord-014597-66vd2mdu	1511	22	growth	growth	NN
cord-014597-66vd2mdu	1511	23	of	of	IN
cord-014597-66vd2mdu	1511	24	CHO	CHO	NNP
cord-014597-66vd2mdu	1511	25	-	-	HYPH
cord-014597-66vd2mdu	1511	26	K1	K1	NNP
cord-014597-66vd2mdu	1511	27	cells	cell	NNS
cord-014597-66vd2mdu	1511	28	,	,	,
cord-014597-66vd2mdu	1511	29	compared	compare	VBN
cord-014597-66vd2mdu	1511	30	to	to	IN
cord-014597-66vd2mdu	1511	31	SFM	SFM	NNP
cord-014597-66vd2mdu	1511	32	-	-	HYPH
cord-014597-66vd2mdu	1511	33	II	II	NNP
cord-014597-66vd2mdu	1511	34	control	control	NN
cord-014597-66vd2mdu	1511	35	media	medium	NNS
cord-014597-66vd2mdu	1511	36	.	.	.
cord-014597-66vd2mdu	1512	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1512	2	feel	feel	VBP
cord-014597-66vd2mdu	1512	3	that	that	IN
cord-014597-66vd2mdu	1512	4	these	these	DT
cord-014597-66vd2mdu	1512	5	early	early	JJ
cord-014597-66vd2mdu	1512	6	-	-	HYPH
cord-014597-66vd2mdu	1512	7	stage	stage	NN
cord-014597-66vd2mdu	1512	8	conditioned	condition	VBN
cord-014597-66vd2mdu	1512	9	media	medium	NNS
cord-014597-66vd2mdu	1512	10	products	product	NNS
cord-014597-66vd2mdu	1512	11	may	may	MD
cord-014597-66vd2mdu	1512	12	increase	increase	VB
cord-014597-66vd2mdu	1512	13	cloning	clone	VBG
cord-014597-66vd2mdu	1512	14	efficiencies	efficiency	NNS
cord-014597-66vd2mdu	1512	15	during	during	IN
cord-014597-66vd2mdu	1512	16	upstream	upstream	JJ
cord-014597-66vd2mdu	1512	17	CHO	CHO	NNP
cord-014597-66vd2mdu	1512	18	cell	cell	NN
cord-014597-66vd2mdu	1512	19	line	line	NN
cord-014597-66vd2mdu	1512	20	development	development	NN
cord-014597-66vd2mdu	1512	21	,	,	,
cord-014597-66vd2mdu	1512	22	resulting	result	VBG
cord-014597-66vd2mdu	1512	23	in	in	IN
cord-014597-66vd2mdu	1512	24	financial	financial	JJ
cord-014597-66vd2mdu	1512	25	savings	saving	NNS
cord-014597-66vd2mdu	1512	26	for	for	IN
cord-014597-66vd2mdu	1512	27	industry	industry	NN
cord-014597-66vd2mdu	1512	28	and	and	CC
cord-014597-66vd2mdu	1512	29	increasing	increase	VBG
cord-014597-66vd2mdu	1512	30	the	the	DT
cord-014597-66vd2mdu	1512	31	possibilities	possibility	NNS
cord-014597-66vd2mdu	1512	32	of	of	IN
cord-014597-66vd2mdu	1512	33	identifying	identify	VBG
cord-014597-66vd2mdu	1512	34	particularly	particularly	RB
cord-014597-66vd2mdu	1512	35	highperforming	highperforme	VBG
cord-014597-66vd2mdu	1512	36	transformed	transform	VBN
cord-014597-66vd2mdu	1512	37	clones	clone	NNS
cord-014597-66vd2mdu	1512	38	.	.	.
cord-014597-66vd2mdu	1513	1	12	12	CD
cord-014597-66vd2mdu	1513	2	(	(	-LRB-
cord-014597-66vd2mdu	1513	3	7):3496	7):3496	CD
cord-014597-66vd2mdu	1513	4	-	-	HYPH
cord-014597-66vd2mdu	1513	5	3510	3510	CD
cord-014597-66vd2mdu	1513	6	.	.	.
cord-014597-66vd2mdu	1514	1	The	the	DT
cord-014597-66vd2mdu	1514	2	main	main	JJ
cord-014597-66vd2mdu	1514	3	rate	rate	NN
cord-014597-66vd2mdu	1514	4	-	-	HYPH
cord-014597-66vd2mdu	1514	5	limiting	limit	VBG
cord-014597-66vd2mdu	1514	6	step	step	NN
cord-014597-66vd2mdu	1514	7	in	in	IN
cord-014597-66vd2mdu	1514	8	the	the	DT
cord-014597-66vd2mdu	1514	9	upstream	upstream	JJ
cord-014597-66vd2mdu	1514	10	stages	stage	NNS
cord-014597-66vd2mdu	1514	11	of	of	IN
cord-014597-66vd2mdu	1514	12	protein	protein	NN
cord-014597-66vd2mdu	1514	13	biomanufacture	biomanufacture	NNP
cord-014597-66vd2mdu	1514	14	is	be	VBZ
cord-014597-66vd2mdu	1514	15	the	the	DT
cord-014597-66vd2mdu	1514	16	isolation	isolation	NN
cord-014597-66vd2mdu	1514	17	of	of	IN
cord-014597-66vd2mdu	1514	18	stable	stable	JJ
cord-014597-66vd2mdu	1514	19	,	,	,
cord-014597-66vd2mdu	1514	20	high	high	JJ
cord-014597-66vd2mdu	1514	21	producing	produce	VBG
cord-014597-66vd2mdu	1514	22	cell	cell	NN
cord-014597-66vd2mdu	1514	23	clones	clone	NNS
cord-014597-66vd2mdu	1514	24	.	.	.
cord-014597-66vd2mdu	1515	1	Ubiquitous	Ubiquitous	NNP
cord-014597-66vd2mdu	1515	2	Chromatin	Chromatin	NNP
cord-014597-66vd2mdu	1515	3	Opening	Opening	NNP
cord-014597-66vd2mdu	1515	4	Elements	Elements	NNP
cord-014597-66vd2mdu	1515	5	(	(	-LRB-
cord-014597-66vd2mdu	1515	6	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1515	7	®	®	POS
cord-014597-66vd2mdu	1515	8	s	s	NN
cord-014597-66vd2mdu	1515	9	)	)	-RRB-
cord-014597-66vd2mdu	1515	10	consist	consist	VBP
cord-014597-66vd2mdu	1515	11	of	of	IN
cord-014597-66vd2mdu	1515	12	at	at	RB
cord-014597-66vd2mdu	1515	13	least	least	RBS
cord-014597-66vd2mdu	1515	14	one	one	CD
cord-014597-66vd2mdu	1515	15	promoter	promoter	NN
cord-014597-66vd2mdu	1515	16	region	region	NN
cord-014597-66vd2mdu	1515	17	with	with	IN
cord-014597-66vd2mdu	1515	18	associated	associated	JJ
cord-014597-66vd2mdu	1515	19	methylation	methylation	NN
cord-014597-66vd2mdu	1515	20	-	-	HYPH
cord-014597-66vd2mdu	1515	21	free	free	JJ
cord-014597-66vd2mdu	1515	22	CpG	CpG	NNP
cord-014597-66vd2mdu	1515	23	island	island	NNP
cord-014597-66vd2mdu	1515	24	from	from	IN
cord-014597-66vd2mdu	1515	25	housekeeping	housekeeping	NN
cord-014597-66vd2mdu	1515	26	genes	gene	NNS
cord-014597-66vd2mdu	1515	27	;	;	:
cord-014597-66vd2mdu	1515	28	they	-PRON-	PRP
cord-014597-66vd2mdu	1515	29	possess	possess	VBP
cord-014597-66vd2mdu	1515	30	a	a	DT
cord-014597-66vd2mdu	1515	31	dominant	dominant	JJ
cord-014597-66vd2mdu	1515	32	chromatin	chromatin	NN
cord-014597-66vd2mdu	1515	33	opening	opening	NN
cord-014597-66vd2mdu	1515	34	capability	capability	NN
cord-014597-66vd2mdu	1515	35	and	and	CC
cord-014597-66vd2mdu	1515	36	thus	thus	RB
cord-014597-66vd2mdu	1515	37	confer	confer	VB
cord-014597-66vd2mdu	1515	38	stable	stable	JJ
cord-014597-66vd2mdu	1515	39	transgene	transgene	NN
cord-014597-66vd2mdu	1515	40	expression	expression	NN
cord-014597-66vd2mdu	1515	41	.	.	.
cord-014597-66vd2mdu	1516	1	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1516	2	®	®	POS
cord-014597-66vd2mdu	1516	3	-viral	-viral	JJ
cord-014597-66vd2mdu	1516	4	promoter	promoter	NN
cord-014597-66vd2mdu	1516	5	(	(	-LRB-
cord-014597-66vd2mdu	1516	6	e.g.	e.g.	RB
cord-014597-66vd2mdu	1516	7	CMV	CMV	NNP
cord-014597-66vd2mdu	1516	8	)	)	-RRB-
cord-014597-66vd2mdu	1516	9	based	base	VBN
cord-014597-66vd2mdu	1516	10	plasmid	plasmid	NN
cord-014597-66vd2mdu	1516	11	vectors	vector	NNS
cord-014597-66vd2mdu	1516	12	markedly	markedly	RB
cord-014597-66vd2mdu	1516	13	reduce	reduce	VBP
cord-014597-66vd2mdu	1516	14	the	the	DT
cord-014597-66vd2mdu	1516	15	time	time	NN
cord-014597-66vd2mdu	1516	16	it	-PRON-	PRP
cord-014597-66vd2mdu	1516	17	takes	take	VBZ
cord-014597-66vd2mdu	1516	18	to	to	TO
cord-014597-66vd2mdu	1516	19	isolate	isolate	VB
cord-014597-66vd2mdu	1516	20	high	high	JJ
cord-014597-66vd2mdu	1516	21	,	,	,
cord-014597-66vd2mdu	1516	22	stably	stably	RB
cord-014597-66vd2mdu	1516	23	producing	produce	VBG
cord-014597-66vd2mdu	1516	24	cell	cell	NN
cord-014597-66vd2mdu	1516	25	clones	clone	NNS
cord-014597-66vd2mdu	1516	26	.	.	.
cord-014597-66vd2mdu	1517	1	Although	although	IN
cord-014597-66vd2mdu	1517	2	some	some	DT
cord-014597-66vd2mdu	1517	3	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1517	4	®	®	POS
cord-014597-66vd2mdu	1517	5	-viral	-viral	JJ
cord-014597-66vd2mdu	1517	6	promoter	promoter	NN
cord-014597-66vd2mdu	1517	7	combinations	combination	NNS
cord-014597-66vd2mdu	1517	8	have	have	VBP
cord-014597-66vd2mdu	1517	9	been	be	VBN
cord-014597-66vd2mdu	1517	10	tested	test	VBN
cord-014597-66vd2mdu	1517	11	,	,	,
cord-014597-66vd2mdu	1517	12	they	-PRON-	PRP
cord-014597-66vd2mdu	1517	13	have	have	VBP
cord-014597-66vd2mdu	1517	14	not	not	RB
cord-014597-66vd2mdu	1517	15	been	be	VBN
cord-014597-66vd2mdu	1517	16	thoroughly	thoroughly	RB
cord-014597-66vd2mdu	1517	17	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1517	18	in	in	IN
cord-014597-66vd2mdu	1517	19	Chinese	chinese	JJ
cord-014597-66vd2mdu	1517	20	hamster	hamster	NN
cord-014597-66vd2mdu	1517	21	ovary	ovary	NN
cord-014597-66vd2mdu	1517	22	(	(	-LRB-
cord-014597-66vd2mdu	1517	23	CHO	CHO	NNP
cord-014597-66vd2mdu	1517	24	)	)	-RRB-
cord-014597-66vd2mdu	1517	25	cells	cell	NNS
cord-014597-66vd2mdu	1517	26	.	.	.
cord-014597-66vd2mdu	1518	1	Plasmid	plasmid	NN
cord-014597-66vd2mdu	1518	2	vectors	vector	NNS
cord-014597-66vd2mdu	1518	3	containing	contain	VBG
cord-014597-66vd2mdu	1518	4	combinations	combination	NNS
cord-014597-66vd2mdu	1518	5	of	of	IN
cord-014597-66vd2mdu	1518	6	either	either	CC
cord-014597-66vd2mdu	1518	7	the	the	DT
cord-014597-66vd2mdu	1518	8	human	human	NN
cord-014597-66vd2mdu	1518	9	HNRPA2B1-CBX3	HNRPA2B1-CBX3	NNP
cord-014597-66vd2mdu	1518	10	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1519	1	®	®	.
cord-014597-66vd2mdu	1520	1	(	(	-LRB-
cord-014597-66vd2mdu	1520	2	A2UCOE	A2UCOE	NNP
cord-014597-66vd2mdu	1520	3	®	®	,
cord-014597-66vd2mdu	1520	4	)	)	-RRB-
cord-014597-66vd2mdu	1520	5	or	or	CC
cord-014597-66vd2mdu	1520	6	murine	murine	JJ
cord-014597-66vd2mdu	1520	7	Rps3	rps3	NN
cord-014597-66vd2mdu	1520	8	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1521	1	®	®	,
cord-014597-66vd2mdu	1521	2	linked	link	VBN
cord-014597-66vd2mdu	1521	3	to	to	IN
cord-014597-66vd2mdu	1521	4	different	different	JJ
cord-014597-66vd2mdu	1521	5	viral	viral	JJ
cord-014597-66vd2mdu	1521	6	promoters	promoter	NNS
cord-014597-66vd2mdu	1521	7	(	(	-LRB-
cord-014597-66vd2mdu	1521	8	hCMV	hcmv	RB
cord-014597-66vd2mdu	1521	9	,	,	,
cord-014597-66vd2mdu	1521	10	gpCMV	gpcmv	NN
cord-014597-66vd2mdu	1521	11	,	,	,
cord-014597-66vd2mdu	1521	12	SFFV	SFFV	NNP
cord-014597-66vd2mdu	1521	13	)	)	-RRB-
cord-014597-66vd2mdu	1521	14	driving	drive	VBG
cord-014597-66vd2mdu	1521	15	expression	expression	NN
cord-014597-66vd2mdu	1521	16	of	of	IN
cord-014597-66vd2mdu	1521	17	an	an	DT
cord-014597-66vd2mdu	1521	18	eGFP	egfp	JJ
cord-014597-66vd2mdu	1521	19	reporter	reporter	NN
cord-014597-66vd2mdu	1521	20	gene	gene	NN
cord-014597-66vd2mdu	1521	21	were	be	VBD
cord-014597-66vd2mdu	1521	22	functionally	functionally	RB
cord-014597-66vd2mdu	1521	23	analysed	analyse	VBN
cord-014597-66vd2mdu	1521	24	by	by	IN
cord-014597-66vd2mdu	1521	25	stable	stable	JJ
cord-014597-66vd2mdu	1521	26	transfection	transfection	NN
cord-014597-66vd2mdu	1521	27	into	into	IN
cord-014597-66vd2mdu	1521	28	CHO	CHO	NNP
cord-014597-66vd2mdu	1521	29	-	-	HYPH
cord-014597-66vd2mdu	1521	30	K1	K1	NNP
cord-014597-66vd2mdu	1521	31	cells	cell	NNS
cord-014597-66vd2mdu	1521	32	and	and	CC
cord-014597-66vd2mdu	1521	33	expression	expression	NN
cord-014597-66vd2mdu	1521	34	analysed	analyse	VBN
cord-014597-66vd2mdu	1521	35	by	by	IN
cord-014597-66vd2mdu	1521	36	flow	flow	NN
cord-014597-66vd2mdu	1521	37	cytometry	cytometry	NN
cord-014597-66vd2mdu	1521	38	and	and	CC
cord-014597-66vd2mdu	1521	39	qPCR	qpcr	NN
cord-014597-66vd2mdu	1521	40	to	to	TO
cord-014597-66vd2mdu	1521	41	determine	determine	VB
cord-014597-66vd2mdu	1521	42	vector	vector	NN
cord-014597-66vd2mdu	1521	43	copy	copy	NN
cord-014597-66vd2mdu	1521	44	number	number	NN
cord-014597-66vd2mdu	1521	45	.	.	.
cord-014597-66vd2mdu	1522	1	The	the	DT
cord-014597-66vd2mdu	1522	2	results	result	NNS
cord-014597-66vd2mdu	1522	3	at	at	IN
cord-014597-66vd2mdu	1522	4	21	21	CD
cord-014597-66vd2mdu	1522	5	days	day	NNS
cord-014597-66vd2mdu	1522	6	post	post	NN
cord-014597-66vd2mdu	1522	7	-	-	JJ
cord-014597-66vd2mdu	1522	8	transfection	transfection	JJ
cord-014597-66vd2mdu	1522	9	and	and	CC
cord-014597-66vd2mdu	1522	10	selection	selection	NN
cord-014597-66vd2mdu	1522	11	clearly	clearly	RB
cord-014597-66vd2mdu	1522	12	indicate	indicate	VBP
cord-014597-66vd2mdu	1522	13	that	that	IN
cord-014597-66vd2mdu	1522	14	the	the	DT
cord-014597-66vd2mdu	1522	15	Rps3	Rps3	NNP
cord-014597-66vd2mdu	1522	16	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1523	1	®	®	.
cord-014597-66vd2mdu	1524	1	-gpCMV	-gpCMV	.
cord-014597-66vd2mdu	1525	1	and	and	CC
cord-014597-66vd2mdu	1525	2	-hCMV	-hcmv	CD
cord-014597-66vd2mdu	1525	3	combinations	combination	NNS
cord-014597-66vd2mdu	1525	4	give	give	VBP
cord-014597-66vd2mdu	1525	5	the	the	DT
cord-014597-66vd2mdu	1525	6	highest	high	JJS
cord-014597-66vd2mdu	1525	7	transgene	transgene	JJ
cord-014597-66vd2mdu	1525	8	expression	expression	NN
cord-014597-66vd2mdu	1525	9	as	as	IN
cord-014597-66vd2mdu	1525	10	shown	show	VBN
cord-014597-66vd2mdu	1525	11	in	in	IN
cord-014597-66vd2mdu	1525	12	Fig	Fig	NNP
cord-014597-66vd2mdu	1525	13	.	.	.
cord-014597-66vd2mdu	1526	1	1	1	LS
cord-014597-66vd2mdu	1526	2	.	.	.
cord-014597-66vd2mdu	1527	1	The	the	DT
cord-014597-66vd2mdu	1527	2	A2UCOE	a2ucoe	NN
cord-014597-66vd2mdu	1527	3	®	®	VBP
cord-014597-66vd2mdu	1527	4	-hCMV	-hcmv	XX
cord-014597-66vd2mdu	1527	5	/	/	NFP
cord-014597-66vd2mdu	1527	6	gpCMV	gpcmv	NN
cord-014597-66vd2mdu	1527	7	constructs	construct	NNS
cord-014597-66vd2mdu	1527	8	were	be	VBD
cord-014597-66vd2mdu	1527	9	the	the	DT
cord-014597-66vd2mdu	1527	10	next	next	JJ
cord-014597-66vd2mdu	1527	11	efficacious	efficacious	JJ
cord-014597-66vd2mdu	1527	12	but	but	CC
cord-014597-66vd2mdu	1527	13	2-fold	2-fold	CD
cord-014597-66vd2mdu	1527	14	lower	low	JJR
cord-014597-66vd2mdu	1527	15	than	than	IN
cord-014597-66vd2mdu	1527	16	the	the	DT
cord-014597-66vd2mdu	1527	17	Rps3	Rps3	NNP
cord-014597-66vd2mdu	1527	18	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1528	1	®	®	NNP
cord-014597-66vd2mdu	1528	2	vectors	vector	NNS
cord-014597-66vd2mdu	1528	3	.	.	.
cord-014597-66vd2mdu	1529	1	The	the	DT
cord-014597-66vd2mdu	1529	2	SFFV	SFFV	NNP
cord-014597-66vd2mdu	1529	3	promoter	promoter	NN
cord-014597-66vd2mdu	1529	4	linked	link	VBN
cord-014597-66vd2mdu	1529	5	with	with	IN
cord-014597-66vd2mdu	1529	6	either	either	DT
cord-014597-66vd2mdu	1529	7	of	of	IN
cord-014597-66vd2mdu	1529	8	the	the	DT
cord-014597-66vd2mdu	1529	9	two	two	CD
cord-014597-66vd2mdu	1529	10	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1530	1	®	®	NNP
cord-014597-66vd2mdu	1530	2	s	s	NNP
cord-014597-66vd2mdu	1530	3	was	be	VBD
cord-014597-66vd2mdu	1530	4	the	the	DT
cord-014597-66vd2mdu	1530	5	least	least	JJS
cord-014597-66vd2mdu	1530	6	effective	effective	JJ
cord-014597-66vd2mdu	1530	7	with	with	IN
cord-014597-66vd2mdu	1530	8	expression	expression	NN
cord-014597-66vd2mdu	1530	9	levels	level	NNS
cord-014597-66vd2mdu	1531	1	17-fold	17-fold	JJ
cord-014597-66vd2mdu	1531	2	lower	low	JJR
cord-014597-66vd2mdu	1531	3	than	than	IN
cord-014597-66vd2mdu	1531	4	the	the	DT
cord-014597-66vd2mdu	1531	5	Rps3-CMV	rps3-cmv	NN
cord-014597-66vd2mdu	1531	6	constructs	construct	NNS
cord-014597-66vd2mdu	1531	7	.	.	.
cord-014597-66vd2mdu	1532	1	The	the	DT
cord-014597-66vd2mdu	1532	2	Rps3	Rps3	NNP
cord-014597-66vd2mdu	1532	3	UCOE	UCOE	NNP
cord-014597-66vd2mdu	1532	4	®	®	.
cord-014597-66vd2mdu	1533	1	-gpCMV	-gpCMV	:
cord-014597-66vd2mdu	1533	2	/	/	SYM
cord-014597-66vd2mdu	1533	3	hCMV	hcmv	NN
cord-014597-66vd2mdu	1533	4	constructs	construct	NNS
cord-014597-66vd2mdu	1533	5	are	be	VBP
cord-014597-66vd2mdu	1533	6	now	now	RB
cord-014597-66vd2mdu	1533	7	being	be	VBG
cord-014597-66vd2mdu	1533	8	further	further	RB
cord-014597-66vd2mdu	1533	9	modified	modify	VBN
cord-014597-66vd2mdu	1533	10	to	to	TO
cord-014597-66vd2mdu	1533	11	include	include	VB
cord-014597-66vd2mdu	1533	12	elements	element	NNS
cord-014597-66vd2mdu	1533	13	that	that	WDT
cord-014597-66vd2mdu	1533	14	will	will	MD
cord-014597-66vd2mdu	1533	15	provide	provide	VB
cord-014597-66vd2mdu	1533	16	optimal	optimal	JJ
cord-014597-66vd2mdu	1533	17	post	post	JJ
cord-014597-66vd2mdu	1533	18	-	-	JJ
cord-014597-66vd2mdu	1533	19	transcriptional	transcriptional	JJ
cord-014597-66vd2mdu	1533	20	pre	pre	JJ
cord-014597-66vd2mdu	1533	21	-	-	JJ
cord-014597-66vd2mdu	1533	22	mRNA	mrna	JJ
cord-014597-66vd2mdu	1533	23	processing	processing	NN
cord-014597-66vd2mdu	1533	24	(	(	-LRB-
cord-014597-66vd2mdu	1533	25	splicing	splicing	NN
cord-014597-66vd2mdu	1533	26	,	,	,
cord-014597-66vd2mdu	1533	27	polyadenylation	polyadenylation	NN
cord-014597-66vd2mdu	1533	28	,	,	,
cord-014597-66vd2mdu	1533	29	transcription	transcription	NN
cord-014597-66vd2mdu	1533	30	termination	termination	NN
cord-014597-66vd2mdu	1533	31	,	,	,
cord-014597-66vd2mdu	1533	32	mRNA	mrna	NN
cord-014597-66vd2mdu	1533	33	stability	stability	NN
cord-014597-66vd2mdu	1533	34	)	)	-RRB-
cord-014597-66vd2mdu	1533	35	thereby	thereby	RB
cord-014597-66vd2mdu	1533	36	maximising	maximise	VBG
cord-014597-66vd2mdu	1533	37	stable	stable	JJ
cord-014597-66vd2mdu	1533	38	cytoplasmic	cytoplasmic	JJ
cord-014597-66vd2mdu	1533	39	transgene	transgene	NN
cord-014597-66vd2mdu	1533	40	mRNA	mrna	NN
cord-014597-66vd2mdu	1533	41	levels	level	NNS
cord-014597-66vd2mdu	1533	42	and	and	CC
cord-014597-66vd2mdu	1533	43	protein	protein	NN
cord-014597-66vd2mdu	1533	44	production	production	NN
cord-014597-66vd2mdu	1533	45	.	.	.
cord-014597-66vd2mdu	1534	1	In	in	IN
cord-014597-66vd2mdu	1534	2	the	the	DT
cord-014597-66vd2mdu	1534	3	last	last	JJ
cord-014597-66vd2mdu	1534	4	20	20	CD
cord-014597-66vd2mdu	1534	5	years	year	NNS
cord-014597-66vd2mdu	1534	6	,	,	,
cord-014597-66vd2mdu	1534	7	growing	grow	VBG
cord-014597-66vd2mdu	1534	8	number	number	NN
cord-014597-66vd2mdu	1534	9	of	of	IN
cord-014597-66vd2mdu	1534	10	innovator	innovator	NN
cord-014597-66vd2mdu	1534	11	biologics	biologic	NNS
cord-014597-66vd2mdu	1534	12	and	and	CC
cord-014597-66vd2mdu	1534	13	biosimilars	biosimilar	NNS
cord-014597-66vd2mdu	1534	14	have	have	VBP
cord-014597-66vd2mdu	1534	15	formed	form	VBN
cord-014597-66vd2mdu	1534	16	a	a	DT
cord-014597-66vd2mdu	1534	17	competitive	competitive	JJ
cord-014597-66vd2mdu	1534	18	environment	environment	NN
cord-014597-66vd2mdu	1534	19	,	,	,
cord-014597-66vd2mdu	1534	20	where	where	WRB
cord-014597-66vd2mdu	1534	21	speed	speed	NN
cord-014597-66vd2mdu	1534	22	and	and	CC
cord-014597-66vd2mdu	1534	23	efficiency	efficiency	NN
cord-014597-66vd2mdu	1534	24	of	of	IN
cord-014597-66vd2mdu	1534	25	generating	generate	VBG
cord-014597-66vd2mdu	1534	26	robust	robust	JJ
cord-014597-66vd2mdu	1534	27	and	and	CC
cord-014597-66vd2mdu	1534	28	highly	highly	RB
cord-014597-66vd2mdu	1534	29	productive	productive	JJ
cord-014597-66vd2mdu	1534	30	cell	cell	NN
cord-014597-66vd2mdu	1534	31	lines	line	NNS
cord-014597-66vd2mdu	1534	32	needs	need	VBZ
cord-014597-66vd2mdu	1534	33	to	to	TO
cord-014597-66vd2mdu	1534	34	be	be	VB
cord-014597-66vd2mdu	1534	35	improved	improve	VBN
cord-014597-66vd2mdu	1534	36	continuously	continuously	RB
cord-014597-66vd2mdu	1534	37	.	.	.
cord-014597-66vd2mdu	1535	1	Through	through	IN
cord-014597-66vd2mdu	1535	2	various	various	JJ
cord-014597-66vd2mdu	1535	3	advances	advance	NNS
cord-014597-66vd2mdu	1535	4	,	,	,
cord-014597-66vd2mdu	1535	5	especially	especially	RB
cord-014597-66vd2mdu	1535	6	in	in	IN
cord-014597-66vd2mdu	1535	7	media	medium	NNS
cord-014597-66vd2mdu	1535	8	development	development	NN
cord-014597-66vd2mdu	1535	9	and	and	CC
cord-014597-66vd2mdu	1535	10	process	process	NN
cord-014597-66vd2mdu	1535	11	optimization	optimization	NN
cord-014597-66vd2mdu	1535	12	,	,	,
cord-014597-66vd2mdu	1535	13	product	product	NN
cord-014597-66vd2mdu	1535	14	titers	titer	NNS
cord-014597-66vd2mdu	1535	15	as	as	RB
cord-014597-66vd2mdu	1535	16	high	high	JJ
cord-014597-66vd2mdu	1535	17	as	as	IN
cord-014597-66vd2mdu	1535	18	10	10	CD
cord-014597-66vd2mdu	1535	19	g	g	NN
cord-014597-66vd2mdu	1535	20	/	/	SYM
cord-014597-66vd2mdu	1535	21	L	L	NNP
cord-014597-66vd2mdu	1535	22	were	be	VBD
cord-014597-66vd2mdu	1535	23	achieved	achieve	VBN
cord-014597-66vd2mdu	1535	24	in	in	IN
cord-014597-66vd2mdu	1535	25	the	the	DT
cord-014597-66vd2mdu	1535	26	pharmaceutical	pharmaceutical	NN
cord-014597-66vd2mdu	1535	27	industry	industry	NN
cord-014597-66vd2mdu	1535	28	(	(	-LRB-
cord-014597-66vd2mdu	1535	29	Kim	Kim	NNP
cord-014597-66vd2mdu	1535	30	et	et	NNP
cord-014597-66vd2mdu	1535	31	al	al	NNP
cord-014597-66vd2mdu	1535	32	.	.	NNP
cord-014597-66vd2mdu	1535	33	,	,	,
cord-014597-66vd2mdu	1535	34	2012	2012	CD
cord-014597-66vd2mdu	1535	35	)	)	-RRB-
cord-014597-66vd2mdu	1535	36	for	for	IN
cord-014597-66vd2mdu	1535	37	standard	standard	JJ
cord-014597-66vd2mdu	1535	38	products	product	NNS
cord-014597-66vd2mdu	1535	39	such	such	JJ
cord-014597-66vd2mdu	1535	40	as	as	IN
cord-014597-66vd2mdu	1535	41	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1535	42	antibodies	antibody	NNS
cord-014597-66vd2mdu	1535	43	.	.	.
cord-014597-66vd2mdu	1536	1	Nevertheless	nevertheless	RB
cord-014597-66vd2mdu	1536	2	,	,	,
cord-014597-66vd2mdu	1536	3	other	other	JJ
cord-014597-66vd2mdu	1536	4	proteins	protein	NNS
cord-014597-66vd2mdu	1537	1	e.g.	e.g.	IN
cord-014597-66vd2mdu	1537	2	bispecific	bispecific	NNP
cord-014597-66vd2mdu	1537	3	antibodies	antibody	NNS
cord-014597-66vd2mdu	1537	4	,	,	,
cord-014597-66vd2mdu	1537	5	Fc	Fc	NNP
cord-014597-66vd2mdu	1537	6	-	-	HYPH
cord-014597-66vd2mdu	1537	7	fusion	fusion	NN
cord-014597-66vd2mdu	1537	8	proteins	protein	NNS
cord-014597-66vd2mdu	1537	9	or	or	CC
cord-014597-66vd2mdu	1537	10	Fab	fab	JJ
cord-014597-66vd2mdu	1537	11	-	-	HYPH
cord-014597-66vd2mdu	1537	12	related	relate	VBN
cord-014597-66vd2mdu	1537	13	products	product	NNS
cord-014597-66vd2mdu	1537	14	are	be	VBP
cord-014597-66vd2mdu	1537	15	difficult	difficult	JJ
cord-014597-66vd2mdu	1537	16	-	-	HYPH
cord-014597-66vd2mdu	1537	17	to	to	TO
cord-014597-66vd2mdu	1537	18	-	-	HYPH
cord-014597-66vd2mdu	1537	19	express	express	VB
cord-014597-66vd2mdu	1537	20	(	(	-LRB-
cord-014597-66vd2mdu	1537	21	DTE	DTE	NNP
cord-014597-66vd2mdu	1537	22	)	)	-RRB-
cord-014597-66vd2mdu	1537	23	in	in	IN
cord-014597-66vd2mdu	1537	24	Chinese	chinese	JJ
cord-014597-66vd2mdu	1537	25	Hamster	Hamster	NNP
cord-014597-66vd2mdu	1537	26	Ovary	Ovary	NNP
cord-014597-66vd2mdu	1537	27	(	(	-LRB-
cord-014597-66vd2mdu	1537	28	CHO	CHO	NNP
cord-014597-66vd2mdu	1537	29	)	)	-RRB-
cord-014597-66vd2mdu	1537	30	and	and	CC
cord-014597-66vd2mdu	1537	31	may	may	MD
cord-014597-66vd2mdu	1537	32	result	result	VB
cord-014597-66vd2mdu	1537	33	in	in	IN
cord-014597-66vd2mdu	1537	34	delays	delay	NNS
cord-014597-66vd2mdu	1537	35	or	or	CC
cord-014597-66vd2mdu	1537	36	even	even	RB
cord-014597-66vd2mdu	1537	37	in	in	IN
cord-014597-66vd2mdu	1537	38	termination	termination	NN
cord-014597-66vd2mdu	1537	39	of	of	IN
cord-014597-66vd2mdu	1537	40	the	the	DT
cord-014597-66vd2mdu	1537	41	cell	cell	NN
cord-014597-66vd2mdu	1537	42	line	line	NN
cord-014597-66vd2mdu	1537	43	development	development	NN
cord-014597-66vd2mdu	1537	44	process	process	NN
cord-014597-66vd2mdu	1537	45	.	.	.
cord-014597-66vd2mdu	1538	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1538	2	developed	develop	VBD
cord-014597-66vd2mdu	1538	3	a	a	DT
cord-014597-66vd2mdu	1538	4	new	new	JJ
cord-014597-66vd2mdu	1538	5	robust	robust	JJ
cord-014597-66vd2mdu	1538	6	pool	pool	NN
cord-014597-66vd2mdu	1538	7	generation	generation	NN
cord-014597-66vd2mdu	1538	8	approach	approach	NN
cord-014597-66vd2mdu	1538	9	(	(	-LRB-
cord-014597-66vd2mdu	1538	10	CLD	CLD	NNP
cord-014597-66vd2mdu	1538	11	2.0	2.0	CD
cord-014597-66vd2mdu	1538	12	)	)	-RRB-
cord-014597-66vd2mdu	1538	13	addressing	address	VBG
cord-014597-66vd2mdu	1538	14	both	both	DT
cord-014597-66vd2mdu	1538	15	,	,	,
cord-014597-66vd2mdu	1538	16	easy	easy	JJ
cord-014597-66vd2mdu	1538	17	-	-	HYPH
cord-014597-66vd2mdu	1538	18	and	and	CC
cord-014597-66vd2mdu	1538	19	difficult	difficult	JJ
cord-014597-66vd2mdu	1538	20	-	-	HYPH
cord-014597-66vd2mdu	1538	21	to	to	TO
cord-014597-66vd2mdu	1538	22	-	-	HYPH
cord-014597-66vd2mdu	1538	23	express	express	NN
cord-014597-66vd2mdu	1538	24	molecules	molecule	NNS
cord-014597-66vd2mdu	1538	25	,	,	,
cord-014597-66vd2mdu	1538	26	while	while	IN
cord-014597-66vd2mdu	1538	27	reducing	reduce	VBG
cord-014597-66vd2mdu	1538	28	timelines	timeline	NNS
cord-014597-66vd2mdu	1538	29	down	down	RB
cord-014597-66vd2mdu	1538	30	to	to	IN
cord-014597-66vd2mdu	1538	31	5	5	CD
cord-014597-66vd2mdu	1538	32	months	month	NNS
cord-014597-66vd2mdu	1538	33	(	(	-LRB-
cord-014597-66vd2mdu	1538	34	CLD	CLD	NNP
cord-014597-66vd2mdu	1538	35	standard	standard	NN
cord-014597-66vd2mdu	1538	36	=	=	SYM
cord-014597-66vd2mdu	1538	37	6	6	CD
cord-014597-66vd2mdu	1538	38	months	month	NNS
cord-014597-66vd2mdu	1538	39	)	)	-RRB-
cord-014597-66vd2mdu	1538	40	,	,	,
cord-014597-66vd2mdu	1538	41	improving	improve	VBG
cord-014597-66vd2mdu	1538	42	reliability	reliability	NN
cord-014597-66vd2mdu	1538	43	of	of	IN
cord-014597-66vd2mdu	1538	44	cell	cell	NN
cord-014597-66vd2mdu	1538	45	line	line	NN
cord-014597-66vd2mdu	1538	46	development	development	NN
cord-014597-66vd2mdu	1538	47	as	as	RB
cord-014597-66vd2mdu	1538	48	well	well	RB
cord-014597-66vd2mdu	1538	49	as	as	IN
cord-014597-66vd2mdu	1538	50	clearly	clearly	RB
cord-014597-66vd2mdu	1538	51	increasing	increase	VBG
cord-014597-66vd2mdu	1538	52	obtained	obtain	VBN
cord-014597-66vd2mdu	1538	53	titers	titer	NNS
cord-014597-66vd2mdu	1538	54	.	.	.
cord-014597-66vd2mdu	1539	1	In	in	IN
cord-014597-66vd2mdu	1539	2	order	order	NN
cord-014597-66vd2mdu	1539	3	to	to	TO
cord-014597-66vd2mdu	1539	4	create	create	VB
cord-014597-66vd2mdu	1539	5	stable	stable	JJ
cord-014597-66vd2mdu	1539	6	cell	cell	NN
cord-014597-66vd2mdu	1539	7	lines	line	NNS
cord-014597-66vd2mdu	1539	8	,	,	,
cord-014597-66vd2mdu	1539	9	we	-PRON-	PRP
cord-014597-66vd2mdu	1539	10	transfected	transfecte	VBD
cord-014597-66vd2mdu	1539	11	our	-PRON-	PRP$
cord-014597-66vd2mdu	1539	12	CHO	CHO	NNP
cord-014597-66vd2mdu	1539	13	DG44	DG44	NNP
cord-014597-66vd2mdu	1539	14	host	host	NN
cord-014597-66vd2mdu	1539	15	cells	cell	NNS
cord-014597-66vd2mdu	1539	16	by	by	IN
cord-014597-66vd2mdu	1539	17	electroporation	electroporation	NN
cord-014597-66vd2mdu	1539	18	.	.	.
cord-014597-66vd2mdu	1540	1	Cells	cell	NNS
cord-014597-66vd2mdu	1540	2	processed	process	VBN
cord-014597-66vd2mdu	1540	3	using	use	VBG
cord-014597-66vd2mdu	1540	4	the	the	DT
cord-014597-66vd2mdu	1540	5	standard	standard	JJ
cord-014597-66vd2mdu	1540	6	approach	approach	NN
cord-014597-66vd2mdu	1540	7	were	be	VBD
cord-014597-66vd2mdu	1540	8	cultivated	cultivate	VBN
cord-014597-66vd2mdu	1540	9	in	in	IN
cord-014597-66vd2mdu	1540	10	selective	selective	JJ
cord-014597-66vd2mdu	1540	11	medium	medium	NN
cord-014597-66vd2mdu	1540	12	or	or	CC
cord-014597-66vd2mdu	1540	13	medium	medium	NN
cord-014597-66vd2mdu	1540	14	containing	contain	VBG
cord-014597-66vd2mdu	1540	15	additional	additional	JJ
cord-014597-66vd2mdu	1540	16	2.5	2.5	CD
cord-014597-66vd2mdu	1540	17	nM	nM	NNP
cord-014597-66vd2mdu	1540	18	methotrexate	methotrexate	NNP
cord-014597-66vd2mdu	1540	19	(	(	-LRB-
cord-014597-66vd2mdu	1540	20	MTX	MTX	NNP
cord-014597-66vd2mdu	1540	21	)	)	-RRB-
cord-014597-66vd2mdu	1540	22	for	for	IN
cord-014597-66vd2mdu	1540	23	three	three	CD
cord-014597-66vd2mdu	1540	24	weeks	week	NNS
cord-014597-66vd2mdu	1540	25	.	.	.
cord-014597-66vd2mdu	1541	1	After	after	IN
cord-014597-66vd2mdu	1541	2	an	an	DT
cord-014597-66vd2mdu	1541	3	amplification	amplification	NN
cord-014597-66vd2mdu	1541	4	step	step	NN
cord-014597-66vd2mdu	1541	5	with	with	IN
cord-014597-66vd2mdu	1541	6	30	30	CD
cord-014597-66vd2mdu	1541	7	nM	nm	NN
cord-014597-66vd2mdu	1541	8	MTX	MTX	NNP
cord-014597-66vd2mdu	1541	9	for	for	IN
cord-014597-66vd2mdu	1541	10	three	three	CD
cord-014597-66vd2mdu	1541	11	weeks	week	NNS
cord-014597-66vd2mdu	1541	12	,	,	,
cord-014597-66vd2mdu	1541	13	stable	stable	JJ
cord-014597-66vd2mdu	1541	14	individual	individual	JJ
cord-014597-66vd2mdu	1541	15	cell	cell	NN
cord-014597-66vd2mdu	1541	16	pools	pool	NNS
cord-014597-66vd2mdu	1541	17	were	be	VBD
cord-014597-66vd2mdu	1541	18	expanded	expand	VBN
cord-014597-66vd2mdu	1541	19	and	and	CC
cord-014597-66vd2mdu	1541	20	clones	clone	NNS
cord-014597-66vd2mdu	1541	21	were	be	VBD
cord-014597-66vd2mdu	1541	22	generated	generate	VBN
cord-014597-66vd2mdu	1541	23	by	by	IN
cord-014597-66vd2mdu	1541	24	FACS	FACS	NNP
cord-014597-66vd2mdu	1541	25	-	-	HYPH
cord-014597-66vd2mdu	1541	26	sorting	sorting	NN
cord-014597-66vd2mdu	1541	27	.	.	.
cord-014597-66vd2mdu	1542	1	Clones	clone	NNS
cord-014597-66vd2mdu	1542	2	were	be	VBD
cord-014597-66vd2mdu	1542	3	analyzed	analyze	VBN
cord-014597-66vd2mdu	1542	4	for	for	IN
cord-014597-66vd2mdu	1542	5	growth	growth	NN
cord-014597-66vd2mdu	1542	6	performance	performance	NN
cord-014597-66vd2mdu	1542	7	and	and	CC
cord-014597-66vd2mdu	1542	8	product	product	NN
cord-014597-66vd2mdu	1542	9	concentration	concentration	NN
cord-014597-66vd2mdu	1542	10	in	in	IN
cord-014597-66vd2mdu	1542	11	fed	fed	NNP
cord-014597-66vd2mdu	1542	12	-	-	HYPH
cord-014597-66vd2mdu	1542	13	batch	batch	NN
cord-014597-66vd2mdu	1542	14	studies	study	NNS
cord-014597-66vd2mdu	1542	15	.	.	.
cord-014597-66vd2mdu	1543	1	In	in	IN
cord-014597-66vd2mdu	1543	2	our	-PRON-	PRP$
cord-014597-66vd2mdu	1543	3	new	new	JJ
cord-014597-66vd2mdu	1543	4	CLD	CLD	NNP
cord-014597-66vd2mdu	1543	5	2.0	2.0	CD
cord-014597-66vd2mdu	1543	6	approach	approach	NN
cord-014597-66vd2mdu	1543	7	,	,	,
cord-014597-66vd2mdu	1543	8	we	-PRON-	PRP
cord-014597-66vd2mdu	1543	9	increased	increase	VBD
cord-014597-66vd2mdu	1543	10	MTX	MTX	NNP
cord-014597-66vd2mdu	1543	11	concentrations	concentration	NNS
cord-014597-66vd2mdu	1543	12	(	(	-LRB-
cord-014597-66vd2mdu	1543	13	2.5	2.5	CD
cord-014597-66vd2mdu	1543	14	nM	nm	NN
cord-014597-66vd2mdu	1543	15	,	,	,
cord-014597-66vd2mdu	1543	16	5	5	CD
cord-014597-66vd2mdu	1543	17	nM	nm	NN
cord-014597-66vd2mdu	1543	18	and	and	CC
cord-014597-66vd2mdu	1543	19	10	10	CD
cord-014597-66vd2mdu	1543	20	nM	nm	NN
cord-014597-66vd2mdu	1543	21	MTX	MTX	NNP
cord-014597-66vd2mdu	1543	22	)	)	-RRB-
cord-014597-66vd2mdu	1543	23	during	during	IN
cord-014597-66vd2mdu	1543	24	the	the	DT
cord-014597-66vd2mdu	1543	25	first	first	JJ
cord-014597-66vd2mdu	1543	26	selection	selection	NN
cord-014597-66vd2mdu	1543	27	phase	phase	NN
cord-014597-66vd2mdu	1543	28	of	of	IN
cord-014597-66vd2mdu	1543	29	three	three	CD
cord-014597-66vd2mdu	1543	30	weeks	week	NNS
cord-014597-66vd2mdu	1543	31	.	.	.
cord-014597-66vd2mdu	1544	1	Afterwards	afterwards	RB
cord-014597-66vd2mdu	1544	2	we	-PRON-	PRP
cord-014597-66vd2mdu	1544	3	omitted	omit	VBD
cord-014597-66vd2mdu	1544	4	the	the	DT
cord-014597-66vd2mdu	1544	5	30	30	CD
cord-014597-66vd2mdu	1544	6	nM	nm	NN
cord-014597-66vd2mdu	1544	7	MTX	MTX	NNP
cord-014597-66vd2mdu	1544	8	amplification	amplification	NN
cord-014597-66vd2mdu	1544	9	step	step	NN
cord-014597-66vd2mdu	1544	10	.	.	.
cord-014597-66vd2mdu	1545	1	Thereby	thereby	RB
cord-014597-66vd2mdu	1545	2	,	,	,
cord-014597-66vd2mdu	1545	3	pool	pool	NN
cord-014597-66vd2mdu	1545	4	generation	generation	NN
cord-014597-66vd2mdu	1545	5	finished	finish	VBD
cord-014597-66vd2mdu	1545	6	four	four	CD
cord-014597-66vd2mdu	1545	7	weeks	week	NNS
cord-014597-66vd2mdu	1545	8	earlier	early	RBR
cord-014597-66vd2mdu	1545	9	than	than	IN
cord-014597-66vd2mdu	1545	10	in	in	IN
cord-014597-66vd2mdu	1545	11	the	the	DT
cord-014597-66vd2mdu	1545	12	standard	standard	JJ
cord-014597-66vd2mdu	1545	13	approach	approach	NN
cord-014597-66vd2mdu	1545	14	.	.	.
cord-014597-66vd2mdu	1546	1	To	to	TO
cord-014597-66vd2mdu	1546	2	evaluate	evaluate	VB
cord-014597-66vd2mdu	1546	3	the	the	DT
cord-014597-66vd2mdu	1546	4	stability	stability	NN
cord-014597-66vd2mdu	1546	5	of	of	IN
cord-014597-66vd2mdu	1546	6	cell	cell	NN
cord-014597-66vd2mdu	1546	7	clones	clone	NNS
cord-014597-66vd2mdu	1546	8	derived	derive	VBN
cord-014597-66vd2mdu	1546	9	from	from	IN
cord-014597-66vd2mdu	1546	10	Mini	Mini	NNP
cord-014597-66vd2mdu	1546	11	Pools	Pools	NNP
cord-014597-66vd2mdu	1546	12	(	(	-LRB-
cord-014597-66vd2mdu	1546	13	MPs	MPs	NNP
cord-014597-66vd2mdu	1546	14	)	)	-RRB-
cord-014597-66vd2mdu	1546	15	generated	generate	VBD
cord-014597-66vd2mdu	1546	16	according	accord	VBG
cord-014597-66vd2mdu	1546	17	to	to	IN
cord-014597-66vd2mdu	1546	18	the	the	DT
cord-014597-66vd2mdu	1546	19	CLD	CLD	NNP
cord-014597-66vd2mdu	1546	20	2.0	2.0	CD
cord-014597-66vd2mdu	1546	21	approach	approach	NN
cord-014597-66vd2mdu	1546	22	,	,	,
cord-014597-66vd2mdu	1546	23	stability	stability	NN
cord-014597-66vd2mdu	1546	24	studies	study	NNS
cord-014597-66vd2mdu	1546	25	were	be	VBD
cord-014597-66vd2mdu	1546	26	performed	perform	VBN
cord-014597-66vd2mdu	1546	27	for	for	IN
cord-014597-66vd2mdu	1546	28	eight	eight	CD
cord-014597-66vd2mdu	1546	29	weeks	week	NNS
cord-014597-66vd2mdu	1546	30	,	,	,
cord-014597-66vd2mdu	1546	31	including	include	VBG
cord-014597-66vd2mdu	1546	32	stability	stability	NN
cord-014597-66vd2mdu	1546	33	fed	feed	VBD
cord-014597-66vd2mdu	1546	34	batches	batch	NNS
cord-014597-66vd2mdu	1546	35	at	at	IN
cord-014597-66vd2mdu	1546	36	t=2	t=2	CD
cord-014597-66vd2mdu	1546	37	weeks	week	NNS
cord-014597-66vd2mdu	1546	38	and	and	CC
cord-014597-66vd2mdu	1546	39	t=8	t=8	JJ
cord-014597-66vd2mdu	1546	40	weeks	week	NNS
cord-014597-66vd2mdu	1546	41	.	.	.
cord-014597-66vd2mdu	1547	1	Altogether	altogether	RB
cord-014597-66vd2mdu	1547	2	three	three	CD
cord-014597-66vd2mdu	1547	3	different	different	JJ
cord-014597-66vd2mdu	1547	4	proteins	protein	NNS
cord-014597-66vd2mdu	1547	5	of	of	IN
cord-014597-66vd2mdu	1547	6	interest	interest	NN
cord-014597-66vd2mdu	1547	7	with	with	IN
cord-014597-66vd2mdu	1547	8	six	six	CD
cord-014597-66vd2mdu	1547	9	cell	cell	NN
cord-014597-66vd2mdu	1547	10	clones	clone	NNS
cord-014597-66vd2mdu	1547	11	each	each	DT
cord-014597-66vd2mdu	1547	12	were	be	VBD
cord-014597-66vd2mdu	1547	13	tested	test	VBN
cord-014597-66vd2mdu	1547	14	.	.	.
cord-014597-66vd2mdu	1548	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1548	2	adapted	adapt	VBD
cord-014597-66vd2mdu	1548	3	our	-PRON-	PRP$
cord-014597-66vd2mdu	1548	4	cell	cell	NN
cord-014597-66vd2mdu	1548	5	line	line	NN
cord-014597-66vd2mdu	1548	6	development	development	NN
cord-014597-66vd2mdu	1548	7	process	process	NN
cord-014597-66vd2mdu	1548	8	by	by	IN
cord-014597-66vd2mdu	1548	9	increasing	increase	VBG
cord-014597-66vd2mdu	1548	10	the	the	DT
cord-014597-66vd2mdu	1548	11	initial	initial	JJ
cord-014597-66vd2mdu	1548	12	selection	selection	NN
cord-014597-66vd2mdu	1548	13	during	during	IN
cord-014597-66vd2mdu	1548	14	the	the	DT
cord-014597-66vd2mdu	1548	15	first	first	JJ
cord-014597-66vd2mdu	1548	16	selection	selection	NN
cord-014597-66vd2mdu	1548	17	phase	phase	NN
cord-014597-66vd2mdu	1548	18	,	,	,
cord-014597-66vd2mdu	1548	19	thereby	thereby	RB
cord-014597-66vd2mdu	1548	20	allowing	allow	VBG
cord-014597-66vd2mdu	1548	21	the	the	DT
cord-014597-66vd2mdu	1548	22	omission	omission	NN
cord-014597-66vd2mdu	1548	23	of	of	IN
cord-014597-66vd2mdu	1548	24	the	the	DT
cord-014597-66vd2mdu	1548	25	30	30	CD
cord-014597-66vd2mdu	1548	26	nM	nm	NN
cord-014597-66vd2mdu	1548	27	MTX	MTX	NNP
cord-014597-66vd2mdu	1548	28	amplification	amplification	NN
cord-014597-66vd2mdu	1548	29	step	step	NN
cord-014597-66vd2mdu	1548	30	.	.	.
cord-014597-66vd2mdu	1549	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1549	2	observed	observe	VBD
cord-014597-66vd2mdu	1549	3	that	that	IN
cord-014597-66vd2mdu	1549	4	the	the	DT
cord-014597-66vd2mdu	1549	5	capacity	capacity	NN
cord-014597-66vd2mdu	1549	6	of	of	IN
cord-014597-66vd2mdu	1549	7	amplifiability	amplifiability	NN
cord-014597-66vd2mdu	1549	8	varied	varied	JJ
cord-014597-66vd2mdu	1549	9	for	for	IN
cord-014597-66vd2mdu	1549	10	different	different	JJ
cord-014597-66vd2mdu	1549	11	products	product	NNS
cord-014597-66vd2mdu	1549	12	.	.	.
cord-014597-66vd2mdu	1550	1	Cell	cell	NN
cord-014597-66vd2mdu	1550	2	lines	line	NNS
cord-014597-66vd2mdu	1550	3	with	with	IN
cord-014597-66vd2mdu	1550	4	a	a	DT
cord-014597-66vd2mdu	1550	5	protein	protein	NN
cord-014597-66vd2mdu	1550	6	titer	titer	NN
cord-014597-66vd2mdu	1550	7	ranging	range	VBG
cord-014597-66vd2mdu	1550	8	from	from	IN
cord-014597-66vd2mdu	1550	9	>	>	XX
cord-014597-66vd2mdu	1550	10	1	1	CD
cord-014597-66vd2mdu	1550	11	g	g	NN
cord-014597-66vd2mdu	1550	12	/	/	SYM
cord-014597-66vd2mdu	1550	13	L	l	NN
cord-014597-66vd2mdu	1550	14	to	to	IN
cord-014597-66vd2mdu	1550	15	1.5	1.5	CD
cord-014597-66vd2mdu	1550	16	g	g	NN
cord-014597-66vd2mdu	1550	17	/	/	SYM
cord-014597-66vd2mdu	1550	18	L	L	NNP
cord-014597-66vd2mdu	1550	19	(	(	-LRB-
cord-014597-66vd2mdu	1550	20	DTE	DTE	NNP
cord-014597-66vd2mdu	1550	21	)	)	-RRB-
cord-014597-66vd2mdu	1550	22	in	in	IN
cord-014597-66vd2mdu	1550	23	shake	shake	NN
cord-014597-66vd2mdu	1550	24	flask	flask	NN
cord-014597-66vd2mdu	1550	25	fed	feed	VBN
cord-014597-66vd2mdu	1550	26	-	-	HYPH
cord-014597-66vd2mdu	1550	27	batch	batch	NN
cord-014597-66vd2mdu	1550	28	showed	show	VBD
cord-014597-66vd2mdu	1550	29	to	to	TO
cord-014597-66vd2mdu	1550	30	be	be	VB
cord-014597-66vd2mdu	1550	31	more	more	RBR
cord-014597-66vd2mdu	1550	32	susceptible	susceptible	JJ
cord-014597-66vd2mdu	1550	33	to	to	IN
cord-014597-66vd2mdu	1550	34	increased	increase	VBN
cord-014597-66vd2mdu	1550	35	initial	initial	JJ
cord-014597-66vd2mdu	1550	36	MTX	MTX	NNP
cord-014597-66vd2mdu	1550	37	levels	level	NNS
cord-014597-66vd2mdu	1550	38	and	and	CC
cord-014597-66vd2mdu	1550	39	were	be	VBD
cord-014597-66vd2mdu	1550	40	thus	thus	RB
cord-014597-66vd2mdu	1550	41	not	not	RB
cord-014597-66vd2mdu	1550	42	amplifiable	amplifiable	JJ
cord-014597-66vd2mdu	1550	43	with	with	IN
cord-014597-66vd2mdu	1550	44	30	30	CD
cord-014597-66vd2mdu	1550	45	nM	nm	NN
cord-014597-66vd2mdu	1550	46	MTX	MTX	NNP
cord-014597-66vd2mdu	1550	47	.	.	.
cord-014597-66vd2mdu	1551	1	In	in	IN
cord-014597-66vd2mdu	1551	2	contrast	contrast	NN
cord-014597-66vd2mdu	1551	3	,	,	,
cord-014597-66vd2mdu	1551	4	cell	cell	NN
cord-014597-66vd2mdu	1551	5	lines	line	NNS
cord-014597-66vd2mdu	1551	6	with	with	IN
cord-014597-66vd2mdu	1551	7	high	high	JJ
cord-014597-66vd2mdu	1551	8	protein	protein	NN
cord-014597-66vd2mdu	1551	9	titer	titer	NN
cord-014597-66vd2mdu	1551	10	>	>	XX
cord-014597-66vd2mdu	1552	1	1.5	1.5	CD
cord-014597-66vd2mdu	1552	2	g	g	NN
cord-014597-66vd2mdu	1552	3	/	/	SYM
cord-014597-66vd2mdu	1552	4	L	L	NNP
cord-014597-66vd2mdu	1552	5	were	be	VBD
cord-014597-66vd2mdu	1552	6	observed	observe	VBN
cord-014597-66vd2mdu	1552	7	to	to	TO
cord-014597-66vd2mdu	1552	8	adapt	adapt	VB
cord-014597-66vd2mdu	1552	9	to	to	IN
cord-014597-66vd2mdu	1552	10	30	30	CD
cord-014597-66vd2mdu	1552	11	nM	nm	NN
cord-014597-66vd2mdu	1552	12	MTX	MTX	NNP
cord-014597-66vd2mdu	1552	13	easily	easily	RB
cord-014597-66vd2mdu	1552	14	and	and	CC
cord-014597-66vd2mdu	1552	15	were	be	VBD
cord-014597-66vd2mdu	1552	16	amplifiable	amplifiable	JJ
cord-014597-66vd2mdu	1552	17	.	.	.
cord-014597-66vd2mdu	1553	1	Finale	finale	NN
cord-014597-66vd2mdu	1553	2	shake	shake	NN
cord-014597-66vd2mdu	1553	3	flask	flask	NN
cord-014597-66vd2mdu	1553	4	fed	feed	VBN
cord-014597-66vd2mdu	1553	5	-	-	HYPH
cord-014597-66vd2mdu	1553	6	batch	batch	NN
cord-014597-66vd2mdu	1553	7	data	datum	NNS
cord-014597-66vd2mdu	1553	8	with	with	IN
cord-014597-66vd2mdu	1553	9	CLD	CLD	NNP
cord-014597-66vd2mdu	1553	10	2.0	2.0	CD
cord-014597-66vd2mdu	1553	11	clones	clone	NNS
cord-014597-66vd2mdu	1553	12	of	of	IN
cord-014597-66vd2mdu	1553	13	highexpressing	highexpresse	VBG
cord-014597-66vd2mdu	1553	14	products	product	NNS
cord-014597-66vd2mdu	1553	15	showed	show	VBD
cord-014597-66vd2mdu	1553	16	comparable	comparable	JJ
cord-014597-66vd2mdu	1553	17	titers	titer	NNS
cord-014597-66vd2mdu	1553	18	to	to	IN
cord-014597-66vd2mdu	1553	19	clones	clone	NNS
cord-014597-66vd2mdu	1553	20	from	from	IN
cord-014597-66vd2mdu	1553	21	the	the	DT
cord-014597-66vd2mdu	1553	22	standard	standard	JJ
cord-014597-66vd2mdu	1553	23	approach	approach	NN
cord-014597-66vd2mdu	1553	24	.	.	.
cord-014597-66vd2mdu	1554	1	CLD	CLD	NNP
cord-014597-66vd2mdu	1554	2	2.0	2.0	CD
cord-014597-66vd2mdu	1554	3	clone	clone	NN
cord-014597-66vd2mdu	1554	4	titers	titer	NNS
cord-014597-66vd2mdu	1554	5	for	for	IN
cord-014597-66vd2mdu	1554	6	DTE	DTE	NNP
cord-014597-66vd2mdu	1554	7	proteins	protein	NNS
cord-014597-66vd2mdu	1554	8	revealed	reveal	VBN
cord-014597-66vd2mdu	1554	9	in	in	IN
cord-014597-66vd2mdu	1554	10	average	average	JJ
cord-014597-66vd2mdu	1554	11	a	a	DT
cord-014597-66vd2mdu	1554	12	2.0-fold	2.0-fold	CD
cord-014597-66vd2mdu	1554	13	increase	increase	NN
cord-014597-66vd2mdu	1554	14	compared	compare	VBN
cord-014597-66vd2mdu	1554	15	to	to	IN
cord-014597-66vd2mdu	1554	16	clones	clone	NNS
cord-014597-66vd2mdu	1554	17	generated	generate	VBN
cord-014597-66vd2mdu	1554	18	in	in	IN
cord-014597-66vd2mdu	1554	19	the	the	DT
cord-014597-66vd2mdu	1554	20	standard	standard	JJ
cord-014597-66vd2mdu	1554	21	approach	approach	NN
cord-014597-66vd2mdu	1554	22	.	.	.
cord-014597-66vd2mdu	1555	1	Titers	titer	NNS
cord-014597-66vd2mdu	1555	2	of	of	IN
cord-014597-66vd2mdu	1555	3	top	top	JJ
cord-014597-66vd2mdu	1555	4	producing	produce	VBG
cord-014597-66vd2mdu	1555	5	clones	clone	NNS
cord-014597-66vd2mdu	1555	6	were	be	VBD
cord-014597-66vd2mdu	1555	7	in	in	IN
cord-014597-66vd2mdu	1555	8	a	a	DT
cord-014597-66vd2mdu	1555	9	range	range	NN
cord-014597-66vd2mdu	1555	10	of	of	IN
cord-014597-66vd2mdu	1555	11	1.8	1.8	CD
cord-014597-66vd2mdu	1555	12	g	g	NN
cord-014597-66vd2mdu	1555	13	/	/	SYM
cord-014597-66vd2mdu	1555	14	L	l	NN
cord-014597-66vd2mdu	1555	15	to	to	IN
cord-014597-66vd2mdu	1555	16	to	to	IN
cord-014597-66vd2mdu	1555	17	2.7	2.7	CD
cord-014597-66vd2mdu	1555	18	g	g	NN
cord-014597-66vd2mdu	1555	19	/	/	SYM
cord-014597-66vd2mdu	1555	20	L	L	NNP
cord-014597-66vd2mdu	1555	21	(	(	-LRB-
cord-014597-66vd2mdu	1555	22	Fig	Fig	NNP
cord-014597-66vd2mdu	1555	23	.	.	.
cord-014597-66vd2mdu	1555	24	1	1	CD
cord-014597-66vd2mdu	1555	25	)	)	-RRB-
cord-014597-66vd2mdu	1555	26	.	.	.
cord-014597-66vd2mdu	1556	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1556	2	,	,	,
cord-014597-66vd2mdu	1556	3	stability	stability	NN
cord-014597-66vd2mdu	1556	4	data	datum	NNS
cord-014597-66vd2mdu	1556	5	of	of	IN
cord-014597-66vd2mdu	1556	6	CLD2.0	cld2.0	JJ
cord-014597-66vd2mdu	1556	7	cell	cell	NN
cord-014597-66vd2mdu	1556	8	clones	clone	NNS
cord-014597-66vd2mdu	1556	9	from	from	IN
cord-014597-66vd2mdu	1556	10	different	different	JJ
cord-014597-66vd2mdu	1556	11	DTE	DTE	NNP
cord-014597-66vd2mdu	1556	12	products	product	NNS
cord-014597-66vd2mdu	1556	13	showed	show	VBD
cord-014597-66vd2mdu	1556	14	a	a	DT
cord-014597-66vd2mdu	1556	15	stable	stable	JJ
cord-014597-66vd2mdu	1556	16	specific	specific	JJ
cord-014597-66vd2mdu	1556	17	productivity	productivity	NN
cord-014597-66vd2mdu	1556	18	in	in	IN
cord-014597-66vd2mdu	1556	19	a	a	DT
cord-014597-66vd2mdu	1556	20	range	range	NN
cord-014597-66vd2mdu	1556	21	of	of	IN
cord-014597-66vd2mdu	1556	22	+	+	SYM
cord-014597-66vd2mdu	1556	23	/-15	/-15	CD
cord-014597-66vd2mdu	1556	24	%	%	NN
cord-014597-66vd2mdu	1556	25	over	over	IN
cord-014597-66vd2mdu	1556	26	eight	eight	CD
cord-014597-66vd2mdu	1556	27	weeks	week	NNS
cord-014597-66vd2mdu	1556	28	cultivation	cultivation	NN
cord-014597-66vd2mdu	1556	29	.	.	.
cord-014597-66vd2mdu	1557	1	Fed	Fed	NNP
cord-014597-66vd2mdu	1557	2	-	-	HYPH
cord-014597-66vd2mdu	1557	3	batch	batch	NN
cord-014597-66vd2mdu	1557	4	titer	titer	NN
cord-014597-66vd2mdu	1557	5	from	from	IN
cord-014597-66vd2mdu	1557	6	t=2	t=2	JJ
cord-014597-66vd2mdu	1557	7	weeks	week	NNS
cord-014597-66vd2mdu	1557	8	and	and	CC
cord-014597-66vd2mdu	1557	9	t=8	t=8	DT
cord-014597-66vd2mdu	1557	10	weeks	week	NNS
cord-014597-66vd2mdu	1557	11	were	be	VBD
cord-014597-66vd2mdu	1557	12	in	in	IN
cord-014597-66vd2mdu	1557	13	a	a	DT
cord-014597-66vd2mdu	1557	14	normal	normal	JJ
cord-014597-66vd2mdu	1557	15	range	range	NN
cord-014597-66vd2mdu	1557	16	of	of	IN
cord-014597-66vd2mdu	1557	17	+	+	NN
cord-014597-66vd2mdu	1557	18	/-20	/-20	CD
cord-014597-66vd2mdu	1557	19	%	%	NN
cord-014597-66vd2mdu	1557	20	of	of	IN
cord-014597-66vd2mdu	1557	21	the	the	DT
cord-014597-66vd2mdu	1557	22	standard	standard	JJ
cord-014597-66vd2mdu	1557	23	30	30	CD
cord-014597-66vd2mdu	1557	24	nM	nm	CD
cord-014597-66vd2mdu	1557	25	projects	project	NNS
cord-014597-66vd2mdu	1557	26	.	.	.
cord-014597-66vd2mdu	1558	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	1558	2	results	result	NNS
cord-014597-66vd2mdu	1558	3	demonstrate	demonstrate	VBP
cord-014597-66vd2mdu	1558	4	that	that	IN
cord-014597-66vd2mdu	1558	5	CLD	CLD	NNP
cord-014597-66vd2mdu	1558	6	2.0	2.0	CD
cord-014597-66vd2mdu	1558	7	is	be	VBZ
cord-014597-66vd2mdu	1558	8	a	a	DT
cord-014597-66vd2mdu	1558	9	robust	robust	JJ
cord-014597-66vd2mdu	1558	10	and	and	CC
cord-014597-66vd2mdu	1558	11	reliable	reliable	JJ
cord-014597-66vd2mdu	1558	12	process	process	NN
cord-014597-66vd2mdu	1558	13	for	for	IN
cord-014597-66vd2mdu	1558	14	standard	standard	JJ
cord-014597-66vd2mdu	1558	15	products	product	NNS
cord-014597-66vd2mdu	1558	16	(	(	-LRB-
cord-014597-66vd2mdu	1558	17	mAb	mab	NN
cord-014597-66vd2mdu	1558	18	)	)	-RRB-
cord-014597-66vd2mdu	1558	19	and	and	CC
cord-014597-66vd2mdu	1558	20	DTE	DTE	NNP
cord-014597-66vd2mdu	1558	21	proteins	protein	NNS
cord-014597-66vd2mdu	1558	22	.	.	.
cord-014597-66vd2mdu	1559	1	With	with	IN
cord-014597-66vd2mdu	1559	2	our	-PRON-	PRP$
cord-014597-66vd2mdu	1559	3	new	new	JJ
cord-014597-66vd2mdu	1559	4	process	process	NN
cord-014597-66vd2mdu	1559	5	,	,	,
cord-014597-66vd2mdu	1559	6	we	-PRON-	PRP
cord-014597-66vd2mdu	1559	7	were	be	VBD
cord-014597-66vd2mdu	1559	8	able	able	JJ
cord-014597-66vd2mdu	1559	9	to	to	TO
cord-014597-66vd2mdu	1559	10	increase	increase	VB
cord-014597-66vd2mdu	1559	11	titer	titer	NN
cord-014597-66vd2mdu	1559	12	of	of	IN
cord-014597-66vd2mdu	1559	13	difficult	difficult	JJ
cord-014597-66vd2mdu	1559	14	-	-	HYPH
cord-014597-66vd2mdu	1559	15	to	to	TO
cord-014597-66vd2mdu	1559	16	-	-	HYPH
cord-014597-66vd2mdu	1559	17	express	express	VB
cord-014597-66vd2mdu	1559	18	proteins	protein	NNS
cord-014597-66vd2mdu	1559	19	up	up	IN
cord-014597-66vd2mdu	1559	20	to	to	IN
cord-014597-66vd2mdu	1559	21	200	200	CD
cord-014597-66vd2mdu	1559	22	%	%	NN
cord-014597-66vd2mdu	1559	23	.	.	.
cord-014597-66vd2mdu	1560	1	By	by	IN
cord-014597-66vd2mdu	1560	2	omitting	omit	VBG
cord-014597-66vd2mdu	1560	3	the	the	DT
cord-014597-66vd2mdu	1560	4	amplification	amplification	NN
cord-014597-66vd2mdu	1560	5	step	step	NN
cord-014597-66vd2mdu	1560	6	(	(	-LRB-
cord-014597-66vd2mdu	1560	7	30	30	CD
cord-014597-66vd2mdu	1560	8	nM	nm	NN
cord-014597-66vd2mdu	1560	9	MTX	MTX	NNP
cord-014597-66vd2mdu	1560	10	)	)	-RRB-
cord-014597-66vd2mdu	1560	11	96	96	CD
cord-014597-66vd2mdu	1560	12	%	%	NN
cord-014597-66vd2mdu	1560	13	of	of	IN
cord-014597-66vd2mdu	1560	14	generated	generate	VBN
cord-014597-66vd2mdu	1560	15	clones	clone	NNS
cord-014597-66vd2mdu	1560	16	were	be	VBD
cord-014597-66vd2mdu	1560	17	stable	stable	JJ
cord-014597-66vd2mdu	1560	18	over	over	IN
cord-014597-66vd2mdu	1560	19	eight	eight	CD
cord-014597-66vd2mdu	1560	20	weeks	week	NNS
cord-014597-66vd2mdu	1560	21	cultivation	cultivation	NN
cord-014597-66vd2mdu	1560	22	time	time	NN
cord-014597-66vd2mdu	1560	23	.	.	.
cord-014597-66vd2mdu	1561	1	Additionally	additionally	RB
cord-014597-66vd2mdu	1561	2	using	use	VBG
cord-014597-66vd2mdu	1561	3	the	the	DT
cord-014597-66vd2mdu	1561	4	CLD2.0	cld2.0	JJ
cord-014597-66vd2mdu	1561	5	approach	approach	NN
cord-014597-66vd2mdu	1561	6	,	,	,
cord-014597-66vd2mdu	1561	7	the	the	DT
cord-014597-66vd2mdu	1561	8	time	time	NN
cord-014597-66vd2mdu	1561	9	line	line	NN
cord-014597-66vd2mdu	1561	10	from	from	IN
cord-014597-66vd2mdu	1561	11	DNA	DNA	NNP
cord-014597-66vd2mdu	1561	12	to	to	IN
cord-014597-66vd2mdu	1561	13	RCB	RCB	NNP
cord-014597-66vd2mdu	1561	14	was	be	VBD
cord-014597-66vd2mdu	1561	15	reduced	reduce	VBN
cord-014597-66vd2mdu	1561	16	to	to	IN
cord-014597-66vd2mdu	1561	17	5	5	CD
cord-014597-66vd2mdu	1561	18	months	month	NNS
cord-014597-66vd2mdu	1561	19	.	.	.
cord-014597-66vd2mdu	1562	1	Background	Background	NNP
cord-014597-66vd2mdu	1562	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1562	3	cells	cell	NNS
cord-014597-66vd2mdu	1562	4	have	have	VBP
cord-014597-66vd2mdu	1562	5	become	become	VBN
cord-014597-66vd2mdu	1562	6	the	the	DT
cord-014597-66vd2mdu	1562	7	most	most	RBS
cord-014597-66vd2mdu	1562	8	popular	popular	JJ
cord-014597-66vd2mdu	1562	9	platform	platform	NN
cord-014597-66vd2mdu	1562	10	for	for	IN
cord-014597-66vd2mdu	1562	11	production	production	NN
cord-014597-66vd2mdu	1562	12	of	of	IN
cord-014597-66vd2mdu	1562	13	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1562	14	proteins	protein	NNS
cord-014597-66vd2mdu	1562	15	[	[	-LRB-
cord-014597-66vd2mdu	1562	16	1	1	CD
cord-014597-66vd2mdu	1562	17	]	]	-RRB-
cord-014597-66vd2mdu	1562	18	.	.	.
cord-014597-66vd2mdu	1563	1	However	however	RB
cord-014597-66vd2mdu	1563	2	,	,	,
cord-014597-66vd2mdu	1563	3	the	the	DT
cord-014597-66vd2mdu	1563	4	generation	generation	NN
cord-014597-66vd2mdu	1563	5	of	of	IN
cord-014597-66vd2mdu	1563	6	high	high	JJ
cord-014597-66vd2mdu	1563	7	-	-	HYPH
cord-014597-66vd2mdu	1563	8	producer	producer	NN
cord-014597-66vd2mdu	1563	9	cells	cell	NNS
cord-014597-66vd2mdu	1563	10	is	be	VBZ
cord-014597-66vd2mdu	1563	11	a	a	DT
cord-014597-66vd2mdu	1563	12	time	time	NN
cord-014597-66vd2mdu	1563	13	-	-	HYPH
cord-014597-66vd2mdu	1563	14	consuming	consume	VBG
cord-014597-66vd2mdu	1563	15	and	and	CC
cord-014597-66vd2mdu	1563	16	labor	labor	NN
cord-014597-66vd2mdu	1563	17	-	-	HYPH
cord-014597-66vd2mdu	1563	18	intensive	intensive	JJ
cord-014597-66vd2mdu	1563	19	process	process	NN
cord-014597-66vd2mdu	1563	20	that	that	WDT
cord-014597-66vd2mdu	1563	21	requires	require	VBZ
cord-014597-66vd2mdu	1563	22	the	the	DT
cord-014597-66vd2mdu	1563	23	screening	screening	NN
cord-014597-66vd2mdu	1563	24	of	of	IN
cord-014597-66vd2mdu	1563	25	large	large	JJ
cord-014597-66vd2mdu	1563	26	amount	amount	NN
cord-014597-66vd2mdu	1563	27	of	of	IN
cord-014597-66vd2mdu	1563	28	cells	cell	NNS
cord-014597-66vd2mdu	1563	29	to	to	TO
cord-014597-66vd2mdu	1563	30	get	get	VB
cord-014597-66vd2mdu	1563	31	a	a	DT
cord-014597-66vd2mdu	1563	32	clone	clone	NN
cord-014597-66vd2mdu	1563	33	of	of	IN
cord-014597-66vd2mdu	1563	34	high	high	JJ
cord-014597-66vd2mdu	1563	35	titer	titer	NN
cord-014597-66vd2mdu	1563	36	and	and	CC
cord-014597-66vd2mdu	1563	37	stability	stability	NN
cord-014597-66vd2mdu	1563	38	.	.	.
cord-014597-66vd2mdu	1564	1	Since	since	IN
cord-014597-66vd2mdu	1564	2	the	the	DT
cord-014597-66vd2mdu	1564	3	expression	expression	NN
cord-014597-66vd2mdu	1564	4	titer	titer	NN
cord-014597-66vd2mdu	1564	5	and	and	CC
cord-014597-66vd2mdu	1564	6	stability	stability	NN
cord-014597-66vd2mdu	1564	7	of	of	IN
cord-014597-66vd2mdu	1564	8	clone	clone	NN
cord-014597-66vd2mdu	1564	9	is	be	VBZ
cord-014597-66vd2mdu	1564	10	highly	highly	RB
cord-014597-66vd2mdu	1564	11	dependent	dependent	JJ
cord-014597-66vd2mdu	1564	12	on	on	IN
cord-014597-66vd2mdu	1564	13	the	the	DT
cord-014597-66vd2mdu	1564	14	site	site	NN
cord-014597-66vd2mdu	1564	15	of	of	IN
cord-014597-66vd2mdu	1564	16	integration	integration	NN
cord-014597-66vd2mdu	1564	17	,	,	,
cord-014597-66vd2mdu	1564	18	we	-PRON-	PRP
cord-014597-66vd2mdu	1564	19	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	1564	20	a	a	DT
cord-014597-66vd2mdu	1564	21	new	new	JJ
cord-014597-66vd2mdu	1564	22	cell	cell	NN
cord-014597-66vd2mdu	1564	23	line	line	NN
cord-014597-66vd2mdu	1564	24	development	development	NN
cord-014597-66vd2mdu	1564	25	strategy	strategy	NN
cord-014597-66vd2mdu	1564	26	by	by	IN
cord-014597-66vd2mdu	1564	27	using	use	VBG
cord-014597-66vd2mdu	1564	28	NGS	NGS	NNP
cord-014597-66vd2mdu	1564	29	to	to	TO
cord-014597-66vd2mdu	1564	30	identify	identify	VB
cord-014597-66vd2mdu	1564	31	the	the	DT
cord-014597-66vd2mdu	1564	32	integration	integration	NN
cord-014597-66vd2mdu	1564	33	site	site	NN
cord-014597-66vd2mdu	1564	34	and	and	CC
cord-014597-66vd2mdu	1564	35	using	use	VBG
cord-014597-66vd2mdu	1564	36	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	1564	37	/	/	SYM
cord-014597-66vd2mdu	1564	38	Cas9	Cas9	NNP
cord-014597-66vd2mdu	1564	39	to	to	TO
cord-014597-66vd2mdu	1564	40	generate	generate	VB
cord-014597-66vd2mdu	1564	41	the	the	DT
cord-014597-66vd2mdu	1564	42	target	target	NN
cord-014597-66vd2mdu	1564	43	integrated	integrate	VBN
cord-014597-66vd2mdu	1564	44	high	high	JJ
cord-014597-66vd2mdu	1564	45	producing	produce	VBG
cord-014597-66vd2mdu	1564	46	cell	cell	NN
cord-014597-66vd2mdu	1564	47	lines	line	NNS
cord-014597-66vd2mdu	1564	48	[	[	-LRB-
cord-014597-66vd2mdu	1564	49	1	1	CD
cord-014597-66vd2mdu	1564	50	,	,	,
cord-014597-66vd2mdu	1564	51	2	2	CD
cord-014597-66vd2mdu	1564	52	]	]	-RRB-
cord-014597-66vd2mdu	1564	53	.	.	.
cord-014597-66vd2mdu	1565	1	To	to	TO
cord-014597-66vd2mdu	1565	2	identify	identify	VB
cord-014597-66vd2mdu	1565	3	the	the	DT
cord-014597-66vd2mdu	1565	4	high	high	JJ
cord-014597-66vd2mdu	1565	5	expression	expression	NN
cord-014597-66vd2mdu	1565	6	sites	site	NNS
cord-014597-66vd2mdu	1565	7	in	in	IN
cord-014597-66vd2mdu	1565	8	the	the	DT
cord-014597-66vd2mdu	1565	9	CHO	CHO	NNP
cord-014597-66vd2mdu	1565	10	cells	cell	NNS
cord-014597-66vd2mdu	1565	11	,	,	,
cord-014597-66vd2mdu	1565	12	we	-PRON-	PRP
cord-014597-66vd2mdu	1565	13	employed	employ	VBD
cord-014597-66vd2mdu	1565	14	NGS	NGS	NNP
cord-014597-66vd2mdu	1565	15	to	to	TO
cord-014597-66vd2mdu	1565	16	analyze	analyze	VB
cord-014597-66vd2mdu	1565	17	the	the	DT
cord-014597-66vd2mdu	1565	18	integration	integration	NN
cord-014597-66vd2mdu	1565	19	sites	site	NNS
cord-014597-66vd2mdu	1565	20	of	of	IN
cord-014597-66vd2mdu	1565	21	a	a	DT
cord-014597-66vd2mdu	1565	22	high	high	JJ
cord-014597-66vd2mdu	1565	23	producing	produce	VBG
cord-014597-66vd2mdu	1565	24	cell	cell	NN
cord-014597-66vd2mdu	1565	25	line	line	NN
cord-014597-66vd2mdu	1565	26	(	(	-LRB-
cord-014597-66vd2mdu	1565	27	titer	titer	NN
cord-014597-66vd2mdu	1565	28	>	>	XX
cord-014597-66vd2mdu	1565	29	3g	3g	NNP
cord-014597-66vd2mdu	1565	30	/	/	SYM
cord-014597-66vd2mdu	1565	31	L	L	NNP
cord-014597-66vd2mdu	1565	32	)	)	-RRB-
cord-014597-66vd2mdu	1565	33	.	.	.
cord-014597-66vd2mdu	1566	1	The	the	DT
cord-014597-66vd2mdu	1566	2	pair	pair	NN
cord-014597-66vd2mdu	1566	3	-	-	HYPH
cord-014597-66vd2mdu	1566	4	end	end	NN
cord-014597-66vd2mdu	1566	5	reads	read	VBZ
cord-014597-66vd2mdu	1566	6	with	with	IN
cord-014597-66vd2mdu	1566	7	one	one	CD
cord-014597-66vd2mdu	1566	8	read	read	NN
cord-014597-66vd2mdu	1566	9	mapped	map	VBN
cord-014597-66vd2mdu	1566	10	to	to	IN
cord-014597-66vd2mdu	1566	11	the	the	DT
cord-014597-66vd2mdu	1566	12	vector	vector	NN
cord-014597-66vd2mdu	1566	13	and	and	CC
cord-014597-66vd2mdu	1566	14	the	the	DT
cord-014597-66vd2mdu	1566	15	other	other	JJ
cord-014597-66vd2mdu	1566	16	read	read	NN
cord-014597-66vd2mdu	1566	17	mapped	map	VBN
cord-014597-66vd2mdu	1566	18	to	to	IN
cord-014597-66vd2mdu	1566	19	the	the	DT
cord-014597-66vd2mdu	1566	20	CHO	CHO	NNP
cord-014597-66vd2mdu	1566	21	reference	reference	NN
cord-014597-66vd2mdu	1566	22	genome	genome	NN
cord-014597-66vd2mdu	1566	23	are	be	VBP
cord-014597-66vd2mdu	1566	24	extracted	extract	VBN
cord-014597-66vd2mdu	1566	25	to	to	TO
cord-014597-66vd2mdu	1566	26	identify	identify	VB
cord-014597-66vd2mdu	1566	27	the	the	DT
cord-014597-66vd2mdu	1566	28	integration	integration	NN
cord-014597-66vd2mdu	1566	29	sites	site	NNS
cord-014597-66vd2mdu	1566	30	.	.	.
cord-014597-66vd2mdu	1567	1	To	to	TO
cord-014597-66vd2mdu	1567	2	test	test	VB
cord-014597-66vd2mdu	1567	3	the	the	DT
cord-014597-66vd2mdu	1567	4	expression	expression	NN
cord-014597-66vd2mdu	1567	5	activity	activity	NN
cord-014597-66vd2mdu	1567	6	of	of	IN
cord-014597-66vd2mdu	1567	7	the	the	DT
cord-014597-66vd2mdu	1567	8	integration	integration	NN
cord-014597-66vd2mdu	1567	9	sites	site	NNS
cord-014597-66vd2mdu	1567	10	,	,	,
cord-014597-66vd2mdu	1567	11	we	-PRON-	PRP
cord-014597-66vd2mdu	1567	12	employed	employ	VBD
cord-014597-66vd2mdu	1567	13	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	1567	14	/	/	SYM
cord-014597-66vd2mdu	1567	15	Cas9	Cas9	NNP
cord-014597-66vd2mdu	1567	16	to	to	TO
cord-014597-66vd2mdu	1567	17	specifically	specifically	RB
cord-014597-66vd2mdu	1567	18	integrate	integrate	VB
cord-014597-66vd2mdu	1567	19	the	the	DT
cord-014597-66vd2mdu	1567	20	antibody	antibody	NN
cord-014597-66vd2mdu	1567	21	gene	gene	NN
cord-014597-66vd2mdu	1567	22	into	into	IN
cord-014597-66vd2mdu	1567	23	CHO	CHO	NNP
cord-014597-66vd2mdu	1567	24	genome	genome	NN
cord-014597-66vd2mdu	1567	25	for	for	IN
cord-014597-66vd2mdu	1567	26	expression	expression	NN
cord-014597-66vd2mdu	1567	27	.	.	.
cord-014597-66vd2mdu	1568	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	1568	2	data	datum	NNS
cord-014597-66vd2mdu	1568	3	showed	show	VBD
cord-014597-66vd2mdu	1568	4	4	4	CD
cord-014597-66vd2mdu	1568	5	integration	integration	NN
cord-014597-66vd2mdu	1568	6	sites	site	NNS
cord-014597-66vd2mdu	1568	7	are	be	VBP
cord-014597-66vd2mdu	1568	8	in	in	IN
cord-014597-66vd2mdu	1568	9	the	the	DT
cord-014597-66vd2mdu	1568	10	high	high	JJ
cord-014597-66vd2mdu	1568	11	producing	produce	VBG
cord-014597-66vd2mdu	1568	12	cell	cell	NN
cord-014597-66vd2mdu	1568	13	line	line	NN
cord-014597-66vd2mdu	1568	14	.	.	.
cord-014597-66vd2mdu	1569	1	Among	among	IN
cord-014597-66vd2mdu	1569	2	the	the	DT
cord-014597-66vd2mdu	1569	3	4	4	CD
cord-014597-66vd2mdu	1569	4	integration	integration	NN
cord-014597-66vd2mdu	1569	5	site	site	NN
cord-014597-66vd2mdu	1569	6	,	,	,
cord-014597-66vd2mdu	1569	7	IS1	is1	NN
cord-014597-66vd2mdu	1569	8	integration	integration	NN
cord-014597-66vd2mdu	1569	9	site	site	NN
cord-014597-66vd2mdu	1569	10	was	be	VBD
cord-014597-66vd2mdu	1569	11	tested	test	VBN
cord-014597-66vd2mdu	1569	12	by	by	IN
cord-014597-66vd2mdu	1569	13	CRISPR/	CRISPR/	NNP
cord-014597-66vd2mdu	1569	14	Cas9	Cas9	NNP
cord-014597-66vd2mdu	1569	15	for	for	IN
cord-014597-66vd2mdu	1569	16	target	target	NN
cord-014597-66vd2mdu	1569	17	integration	integration	NN
cord-014597-66vd2mdu	1569	18	of	of	IN
cord-014597-66vd2mdu	1569	19	antibody	antibody	NN
cord-014597-66vd2mdu	1569	20	gene	gene	NN
cord-014597-66vd2mdu	1569	21	for	for	IN
cord-014597-66vd2mdu	1569	22	expression	expression	NN
cord-014597-66vd2mdu	1569	23	.	.	.
cord-014597-66vd2mdu	1570	1	The	the	DT
cord-014597-66vd2mdu	1570	2	IS1target	IS1target	NNP
cord-014597-66vd2mdu	1570	3	integrated	integrate	VBD
cord-014597-66vd2mdu	1570	4	cell	cell	NN
cord-014597-66vd2mdu	1570	5	pool	pool	NN
cord-014597-66vd2mdu	1570	6	present	present	VBP
cord-014597-66vd2mdu	1570	7	higher	high	JJR
cord-014597-66vd2mdu	1570	8	expression	expression	NN
cord-014597-66vd2mdu	1570	9	titer	titer	NN
cord-014597-66vd2mdu	1570	10	than	than	IN
cord-014597-66vd2mdu	1570	11	cell	cell	NN
cord-014597-66vd2mdu	1570	12	pool	pool	NN
cord-014597-66vd2mdu	1570	13	generated	generate	VBN
cord-014597-66vd2mdu	1570	14	by	by	IN
cord-014597-66vd2mdu	1570	15	target	target	NN
cord-014597-66vd2mdu	1570	16	integration	integration	NN
cord-014597-66vd2mdu	1570	17	into	into	IN
cord-014597-66vd2mdu	1570	18	other	other	JJ
cord-014597-66vd2mdu	1570	19	integration	integration	NN
cord-014597-66vd2mdu	1570	20	sites	site	NNS
cord-014597-66vd2mdu	1570	21	(	(	-LRB-
cord-014597-66vd2mdu	1570	22	Fig	Fig	NNP
cord-014597-66vd2mdu	1570	23	.	.	.
cord-014597-66vd2mdu	1570	24	1a	1a	CD
cord-014597-66vd2mdu	1570	25	)	)	-RRB-
cord-014597-66vd2mdu	1570	26	.	.	.
cord-014597-66vd2mdu	1571	1	The	the	DT
cord-014597-66vd2mdu	1571	2	single	single	JJ
cord-014597-66vd2mdu	1571	3	cell	cell	NN
cord-014597-66vd2mdu	1571	4	clones	clone	NNS
cord-014597-66vd2mdu	1571	5	derived	derive	VBN
cord-014597-66vd2mdu	1571	6	from	from	IN
cord-014597-66vd2mdu	1571	7	IS1-target	is1-target	DT
cord-014597-66vd2mdu	1571	8	integrated	integrated	JJ
cord-014597-66vd2mdu	1571	9	cell	cell	NN
cord-014597-66vd2mdu	1571	10	pool	pool	NN
cord-014597-66vd2mdu	1571	11	had	have	VBD
cord-014597-66vd2mdu	1571	12	low	low	JJ
cord-014597-66vd2mdu	1571	13	copy	copy	NN
cord-014597-66vd2mdu	1571	14	number	number	NN
cord-014597-66vd2mdu	1571	15	of	of	IN
cord-014597-66vd2mdu	1571	16	GOI	GOI	NNP
cord-014597-66vd2mdu	1571	17	(	(	-LRB-
cord-014597-66vd2mdu	1571	18	Fig	fig	NN
cord-014597-66vd2mdu	1571	19	.	.	.
cord-014597-66vd2mdu	1571	20	1b	1b	CD
cord-014597-66vd2mdu	1571	21	)	)	-RRB-
cord-014597-66vd2mdu	1571	22	.	.	.
cord-014597-66vd2mdu	1572	1	After	after	IN
cord-014597-66vd2mdu	1572	2	normalization	normalization	NN
cord-014597-66vd2mdu	1572	3	with	with	IN
cord-014597-66vd2mdu	1572	4	copy	copy	JJ
cord-014597-66vd2mdu	1572	5	numbers	number	NNS
cord-014597-66vd2mdu	1572	6	,	,	,
cord-014597-66vd2mdu	1572	7	the	the	DT
cord-014597-66vd2mdu	1572	8	single	single	JJ
cord-014597-66vd2mdu	1572	9	cell	cell	NN
cord-014597-66vd2mdu	1572	10	clones	clone	NNS
cord-014597-66vd2mdu	1572	11	derived	derive	VBN
cord-014597-66vd2mdu	1572	12	from	from	IN
cord-014597-66vd2mdu	1572	13	IS1-target	is1-target	DT
cord-014597-66vd2mdu	1572	14	integrated	integrated	JJ
cord-014597-66vd2mdu	1572	15	cell	cell	NN
cord-014597-66vd2mdu	1572	16	pool	pool	NN
cord-014597-66vd2mdu	1572	17	showed	show	VBD
cord-014597-66vd2mdu	1572	18	high	high	JJ
cord-014597-66vd2mdu	1572	19	titer	titer	NN
cord-014597-66vd2mdu	1572	20	per	per	IN
cord-014597-66vd2mdu	1572	21	copy	copy	NN
cord-014597-66vd2mdu	1572	22	(	(	-LRB-
cord-014597-66vd2mdu	1572	23	123~583	123~583	CD
cord-014597-66vd2mdu	1572	24	mg	mg	NNP
cord-014597-66vd2mdu	1572	25	/	/	SYM
cord-014597-66vd2mdu	1572	26	L	L	NNP
cord-014597-66vd2mdu	1572	27	/	/	SYM
cord-014597-66vd2mdu	1572	28	copy	copy	NN
cord-014597-66vd2mdu	1572	29	)	)	-RRB-
cord-014597-66vd2mdu	1573	1	(	(	-LRB-
cord-014597-66vd2mdu	1573	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1573	3	.	.	.
cord-014597-66vd2mdu	1573	4	1c	1c	CD
cord-014597-66vd2mdu	1573	5	)	)	-RRB-
cord-014597-66vd2mdu	1573	6	.	.	.
cord-014597-66vd2mdu	1574	1	This	this	DT
cord-014597-66vd2mdu	1574	2	study	study	NN
cord-014597-66vd2mdu	1574	3	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	1574	4	the	the	DT
cord-014597-66vd2mdu	1574	5	generation	generation	NN
cord-014597-66vd2mdu	1574	6	of	of	IN
cord-014597-66vd2mdu	1574	7	high	high	RB
cord-014597-66vd2mdu	1574	8	-	-	HYPH
cord-014597-66vd2mdu	1574	9	producing	produce	VBG
cord-014597-66vd2mdu	1574	10	cell	cell	NN
cord-014597-66vd2mdu	1574	11	lines	line	NNS
cord-014597-66vd2mdu	1574	12	by	by	IN
cord-014597-66vd2mdu	1574	13	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	1574	14	/	/	SYM
cord-014597-66vd2mdu	1574	15	Cas9	Cas9	NNP
cord-014597-66vd2mdu	1574	16	mediated	mediate	VBN
cord-014597-66vd2mdu	1574	17	target	target	NN
cord-014597-66vd2mdu	1574	18	integration	integration	NN
cord-014597-66vd2mdu	1574	19	.	.	.
cord-014597-66vd2mdu	1575	1	This	this	DT
cord-014597-66vd2mdu	1575	2	approach	approach	NN
cord-014597-66vd2mdu	1575	3	will	will	MD
cord-014597-66vd2mdu	1575	4	cost	cost	VB
cord-014597-66vd2mdu	1575	5	less	less	JJR
cord-014597-66vd2mdu	1575	6	time	time	NN
cord-014597-66vd2mdu	1575	7	and	and	CC
cord-014597-66vd2mdu	1575	8	labor	labor	NN
cord-014597-66vd2mdu	1575	9	than	than	IN
cord-014597-66vd2mdu	1575	10	traditional	traditional	JJ
cord-014597-66vd2mdu	1575	11	method	method	NN
cord-014597-66vd2mdu	1575	12	.	.	.
cord-014597-66vd2mdu	1576	1	The	the	DT
cord-014597-66vd2mdu	1576	2	active	active	JJ
cord-014597-66vd2mdu	1576	3	integration	integration	NN
cord-014597-66vd2mdu	1576	4	site	site	NN
cord-014597-66vd2mdu	1576	5	will	will	MD
cord-014597-66vd2mdu	1576	6	serve	serve	VB
cord-014597-66vd2mdu	1576	7	as	as	IN
cord-014597-66vd2mdu	1576	8	a	a	DT
cord-014597-66vd2mdu	1576	9	platform	platform	NN
cord-014597-66vd2mdu	1576	10	like	like	IN
cord-014597-66vd2mdu	1576	11	a	a	DT
cord-014597-66vd2mdu	1576	12	cassette	cassette	NN
cord-014597-66vd2mdu	1576	13	player	player	NN
cord-014597-66vd2mdu	1576	14	for	for	IN
cord-014597-66vd2mdu	1576	15	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1576	16	antibody	antibody	NN
cord-014597-66vd2mdu	1576	17	production	production	NN
cord-014597-66vd2mdu	1576	18	.	.	.
cord-014597-66vd2mdu	1577	1	Background	Background	NNP
cord-014597-66vd2mdu	1577	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1577	3	,	,	,
cord-014597-66vd2mdu	1577	4	HEK	HEK	NNP
cord-014597-66vd2mdu	1577	5	and	and	CC
cord-014597-66vd2mdu	1577	6	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1577	7	are	be	VBP
cord-014597-66vd2mdu	1577	8	the	the	DT
cord-014597-66vd2mdu	1577	9	dominant	dominant	JJ
cord-014597-66vd2mdu	1577	10	host	host	NN
cord-014597-66vd2mdu	1577	11	cells	cell	NNS
cord-014597-66vd2mdu	1577	12	for	for	IN
cord-014597-66vd2mdu	1577	13	biologics	biologic	NNS
cord-014597-66vd2mdu	1577	14	drug	drug	NN
cord-014597-66vd2mdu	1577	15	production	production	NN
cord-014597-66vd2mdu	1577	16	.	.	.
cord-014597-66vd2mdu	1578	1	Achieving	achieve	VBG
cord-014597-66vd2mdu	1578	2	high	high	JJ
cord-014597-66vd2mdu	1578	3	level	level	NN
cord-014597-66vd2mdu	1578	4	of	of	IN
cord-014597-66vd2mdu	1578	5	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1578	6	protein	protein	NN
cord-014597-66vd2mdu	1578	7	production	production	NN
cord-014597-66vd2mdu	1578	8	by	by	IN
cord-014597-66vd2mdu	1578	9	these	these	DT
cord-014597-66vd2mdu	1578	10	cell	cell	NN
cord-014597-66vd2mdu	1578	11	lines	line	NNS
cord-014597-66vd2mdu	1578	12	still	still	RB
cord-014597-66vd2mdu	1578	13	remains	remain	VBZ
cord-014597-66vd2mdu	1578	14	a	a	DT
cord-014597-66vd2mdu	1578	15	challenge	challenge	NN
cord-014597-66vd2mdu	1578	16	.	.	.
cord-014597-66vd2mdu	1579	1	In	in	IN
cord-014597-66vd2mdu	1579	2	order	order	NN
cord-014597-66vd2mdu	1579	3	to	to	TO
cord-014597-66vd2mdu	1579	4	understand	understand	VB
cord-014597-66vd2mdu	1579	5	the	the	DT
cord-014597-66vd2mdu	1579	6	potential	potential	JJ
cord-014597-66vd2mdu	1579	7	roles	role	NNS
cord-014597-66vd2mdu	1579	8	of	of	IN
cord-014597-66vd2mdu	1579	9	lipids	lipid	NNS
cord-014597-66vd2mdu	1579	10	in	in	IN
cord-014597-66vd2mdu	1579	11	protein	protein	NN
cord-014597-66vd2mdu	1579	12	production	production	NN
cord-014597-66vd2mdu	1579	13	,	,	,
cord-014597-66vd2mdu	1579	14	secretion	secretion	NN
cord-014597-66vd2mdu	1579	15	,	,	,
cord-014597-66vd2mdu	1579	16	vesicular	vesicular	JJ
cord-014597-66vd2mdu	1579	17	transport	transport	NN
cord-014597-66vd2mdu	1579	18	and	and	CC
cord-014597-66vd2mdu	1579	19	energy	energy	NN
cord-014597-66vd2mdu	1579	20	metabolism	metabolism	NN
cord-014597-66vd2mdu	1579	21	,	,	,
cord-014597-66vd2mdu	1579	22	we	-PRON-	PRP
cord-014597-66vd2mdu	1579	23	coupled	couple	VBD
cord-014597-66vd2mdu	1579	24	high	high	JJ
cord-014597-66vd2mdu	1579	25	-	-	HYPH
cord-014597-66vd2mdu	1579	26	throughput	throughput	NN
cord-014597-66vd2mdu	1579	27	transcriptomics	transcriptomic	NNS
cord-014597-66vd2mdu	1579	28	and	and	CC
cord-014597-66vd2mdu	1579	29	lipidomics	lipidomics	NNP
cord-014597-66vd2mdu	1579	30	technologies	technology	NNS
cord-014597-66vd2mdu	1579	31	.	.	.
cord-014597-66vd2mdu	1580	1	Quantitative	quantitative	JJ
cord-014597-66vd2mdu	1580	2	lipidomics	lipidomic	NNS
cord-014597-66vd2mdu	1580	3	is	be	VBZ
cord-014597-66vd2mdu	1580	4	an	an	DT
cord-014597-66vd2mdu	1580	5	emerging	emerge	VBG
cord-014597-66vd2mdu	1580	6	'	'	``
cord-014597-66vd2mdu	1580	7	omics	omic	NNS
cord-014597-66vd2mdu	1580	8	technology	technology	NN
cord-014597-66vd2mdu	1580	9	which	which	WDT
cord-014597-66vd2mdu	1580	10	can	can	MD
cord-014597-66vd2mdu	1580	11	help	help	VB
cord-014597-66vd2mdu	1580	12	us	-PRON-	PRP
cord-014597-66vd2mdu	1580	13	understand	understand	VB
cord-014597-66vd2mdu	1580	14	the	the	DT
cord-014597-66vd2mdu	1580	15	physiological	physiological	JJ
cord-014597-66vd2mdu	1580	16	limitations	limitation	NNS
cord-014597-66vd2mdu	1580	17	of	of	IN
cord-014597-66vd2mdu	1580	18	each	each	DT
cord-014597-66vd2mdu	1580	19	cell	cell	NN
cord-014597-66vd2mdu	1580	20	line	line	NN
cord-014597-66vd2mdu	1580	21	.	.	.
cord-014597-66vd2mdu	1581	1	The	the	DT
cord-014597-66vd2mdu	1581	2	two	two	CD
cord-014597-66vd2mdu	1581	3	types	type	NNS
cord-014597-66vd2mdu	1581	4	of	of	IN
cord-014597-66vd2mdu	1581	5	major	major	JJ
cord-014597-66vd2mdu	1581	6	lipid	lipid	NN
cord-014597-66vd2mdu	1581	7	groups	group	NNS
cord-014597-66vd2mdu	1581	8	in	in	IN
cord-014597-66vd2mdu	1581	9	cells	cell	NNS
cord-014597-66vd2mdu	1581	10	are	be	VBP
cord-014597-66vd2mdu	1581	11	non	non	JJ
cord-014597-66vd2mdu	1581	12	-	-	JJ
cord-014597-66vd2mdu	1581	13	polar	polar	JJ
cord-014597-66vd2mdu	1581	14	and	and	CC
cord-014597-66vd2mdu	1581	15	polar	polar	JJ
cord-014597-66vd2mdu	1581	16	lipids	lipid	NNS
cord-014597-66vd2mdu	1581	17	.	.	.
cord-014597-66vd2mdu	1582	1	Polar	polar	JJ
cord-014597-66vd2mdu	1582	2	lipids	lipid	NNS
cord-014597-66vd2mdu	1582	3	such	such	JJ
cord-014597-66vd2mdu	1582	4	as	as	IN
cord-014597-66vd2mdu	1582	5	glycerophospholipids	glycerophospholipid	NNS
cord-014597-66vd2mdu	1582	6	(	(	-LRB-
cord-014597-66vd2mdu	1582	7	PLs	pl	NNS
cord-014597-66vd2mdu	1582	8	)	)	-RRB-
cord-014597-66vd2mdu	1582	9	include	include	VBP
cord-014597-66vd2mdu	1582	10	phosphatidylethanolamine	phosphatidylethanolamine	JJ
cord-014597-66vd2mdu	1582	11	(	(	-LRB-
cord-014597-66vd2mdu	1582	12	PE	PE	NNP
cord-014597-66vd2mdu	1582	13	)	)	-RRB-
cord-014597-66vd2mdu	1582	14	,	,	,
cord-014597-66vd2mdu	1582	15	phosphatidylcholine	phosphatidylcholine	NN
cord-014597-66vd2mdu	1582	16	(	(	-LRB-
cord-014597-66vd2mdu	1582	17	PC	pc	NN
cord-014597-66vd2mdu	1582	18	)	)	-RRB-
cord-014597-66vd2mdu	1582	19	,	,	,
cord-014597-66vd2mdu	1582	20	phosphatidylinositol	phosphatidylinositol	JJ
cord-014597-66vd2mdu	1582	21	(	(	-LRB-
cord-014597-66vd2mdu	1582	22	PI	PI	NNP
cord-014597-66vd2mdu	1582	23	)	)	-RRB-
cord-014597-66vd2mdu	1582	24	,	,	,
cord-014597-66vd2mdu	1582	25	phosphatidylserine	phosphatidylserine	NN
cord-014597-66vd2mdu	1582	26	(	(	-LRB-
cord-014597-66vd2mdu	1582	27	PS	PS	NNP
cord-014597-66vd2mdu	1582	28	)	)	-RRB-
cord-014597-66vd2mdu	1582	29	,	,	,
cord-014597-66vd2mdu	1582	30	phosphatidylglycerol	phosphatidylglycerol	NNP
cord-014597-66vd2mdu	1582	31	(	(	-LRB-
cord-014597-66vd2mdu	1582	32	PG	PG	NNP
cord-014597-66vd2mdu	1582	33	)	)	-RRB-
cord-014597-66vd2mdu	1582	34	,	,	,
cord-014597-66vd2mdu	1582	35	and	and	CC
cord-014597-66vd2mdu	1582	36	phosphatidic	phosphatidic	JJ
cord-014597-66vd2mdu	1582	37	acid	acid	NN
cord-014597-66vd2mdu	1582	38	(	(	-LRB-
cord-014597-66vd2mdu	1582	39	PA	PA	NNP
cord-014597-66vd2mdu	1582	40	)	)	-RRB-
cord-014597-66vd2mdu	1582	41	.	.	.
cord-014597-66vd2mdu	1583	1	In	in	IN
cord-014597-66vd2mdu	1583	2	this	this	DT
cord-014597-66vd2mdu	1583	3	study	study	NN
cord-014597-66vd2mdu	1583	4	;	;	:
cord-014597-66vd2mdu	1583	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1583	6	integrated	integrate	VBD
cord-014597-66vd2mdu	1583	7	two	two	CD
cord-014597-66vd2mdu	1583	8	dimensional	dimensional	JJ
cord-014597-66vd2mdu	1583	9	high	high	JJ
cord-014597-66vd2mdu	1583	10	performance	performance	NN
cord-014597-66vd2mdu	1583	11	thin	thin	JJ
cord-014597-66vd2mdu	1583	12	layer	layer	NN
cord-014597-66vd2mdu	1583	13	chromatography	chromatography	NN
cord-014597-66vd2mdu	1583	14	(	(	-LRB-
cord-014597-66vd2mdu	1583	15	2D	2D	NNP
cord-014597-66vd2mdu	1583	16	-	-	HYPH
cord-014597-66vd2mdu	1583	17	HPTLC	HPTLC	NNP
cord-014597-66vd2mdu	1583	18	)	)	-RRB-
cord-014597-66vd2mdu	1583	19	and	and	CC
cord-014597-66vd2mdu	1583	20	mass	mass	NN
cord-014597-66vd2mdu	1583	21	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	1583	22	(	(	-LRB-
cord-014597-66vd2mdu	1583	23	MS	MS	NNP
cord-014597-66vd2mdu	1583	24	)	)	-RRB-
cord-014597-66vd2mdu	1583	25	lipid	lipid	NN
cord-014597-66vd2mdu	1583	26	analysis	analysis	NN
cord-014597-66vd2mdu	1583	27	of	of	IN
cord-014597-66vd2mdu	1583	28	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1583	29	,	,	,
cord-014597-66vd2mdu	1583	30	CHO	CHO	NNP
cord-014597-66vd2mdu	1583	31	,	,	,
cord-014597-66vd2mdu	1583	32	and	and	CC
cord-014597-66vd2mdu	1583	33	HEK	HEK	NNP
cord-014597-66vd2mdu	1583	34	cell	cell	NN
cord-014597-66vd2mdu	1583	35	lines	line	NNS
cord-014597-66vd2mdu	1583	36	to	to	TO
cord-014597-66vd2mdu	1583	37	understand	understand	VB
cord-014597-66vd2mdu	1583	38	the	the	DT
cord-014597-66vd2mdu	1583	39	major	major	JJ
cord-014597-66vd2mdu	1583	40	differences	difference	NNS
cord-014597-66vd2mdu	1583	41	in	in	IN
cord-014597-66vd2mdu	1583	42	the	the	DT
cord-014597-66vd2mdu	1583	43	lipid	lipid	NN
cord-014597-66vd2mdu	1583	44	content	content	NN
cord-014597-66vd2mdu	1583	45	of	of	IN
cord-014597-66vd2mdu	1583	46	these	these	DT
cord-014597-66vd2mdu	1583	47	hosts	host	NNS
cord-014597-66vd2mdu	1583	48	.	.	.
cord-014597-66vd2mdu	1584	1	Bligh	Bligh	NNP
cord-014597-66vd2mdu	1584	2	-	-	HYPH
cord-014597-66vd2mdu	1584	3	Dyer	Dyer	NNP
cord-014597-66vd2mdu	1584	4	method	method	NN
cord-014597-66vd2mdu	1584	5	was	be	VBD
cord-014597-66vd2mdu	1584	6	used	use	VBN
cord-014597-66vd2mdu	1584	7	to	to	TO
cord-014597-66vd2mdu	1584	8	extract	extract	VB
cord-014597-66vd2mdu	1584	9	the	the	DT
cord-014597-66vd2mdu	1584	10	lipids	lipid	NNS
cord-014597-66vd2mdu	1584	11	and	and	CC
cord-014597-66vd2mdu	1584	12	extracts	extract	NNS
cord-014597-66vd2mdu	1584	13	were	be	VBD
cord-014597-66vd2mdu	1584	14	analyzed	analyze	VBN
cord-014597-66vd2mdu	1584	15	by	by	IN
cord-014597-66vd2mdu	1584	16	HP	HP	NNP
cord-014597-66vd2mdu	1584	17	-	-	HYPH
cord-014597-66vd2mdu	1584	18	TLC	TLC	NNP
cord-014597-66vd2mdu	1584	19	and	and	CC
cord-014597-66vd2mdu	1584	20	MS	MS	NNP
cord-014597-66vd2mdu	1584	21	.	.	.
cord-014597-66vd2mdu	1585	1	The	the	DT
cord-014597-66vd2mdu	1585	2	polar	polar	JJ
cord-014597-66vd2mdu	1585	3	lipids	lipid	NNS
cord-014597-66vd2mdu	1585	4	were	be	VBD
cord-014597-66vd2mdu	1585	5	separated	separate	VBN
cord-014597-66vd2mdu	1585	6	into	into	IN
cord-014597-66vd2mdu	1585	7	different	different	JJ
cord-014597-66vd2mdu	1585	8	categories	category	NNS
cord-014597-66vd2mdu	1585	9	by	by	IN
cord-014597-66vd2mdu	1585	10	2-D	2-d	JJ
cord-014597-66vd2mdu	1585	11	HP	HP	NNP
cord-014597-66vd2mdu	1585	12	-	-	HYPH
cord-014597-66vd2mdu	1585	13	TLC	TLC	NNP
cord-014597-66vd2mdu	1585	14	using	use	VBG
cord-014597-66vd2mdu	1585	15	a	a	DT
cord-014597-66vd2mdu	1585	16	CHCl	CHCl	NNS
cord-014597-66vd2mdu	1585	17	3	3	CD
cord-014597-66vd2mdu	1585	18	-MeOH	-MeOH	NNS
cord-014597-66vd2mdu	1585	19	-	-	HYPH
cord-014597-66vd2mdu	1585	20	H	h	NN
cord-014597-66vd2mdu	1585	21	2	2	CD
cord-014597-66vd2mdu	1585	22	O	o	NN
cord-014597-66vd2mdu	1585	23	(	(	-LRB-
cord-014597-66vd2mdu	1585	24	71:25:2.5	71:25:2.5	NNP
cord-014597-66vd2mdu	1585	25	,	,	,
cord-014597-66vd2mdu	1585	26	v	v	NNP
cord-014597-66vd2mdu	1585	27	/	/	SYM
cord-014597-66vd2mdu	1585	28	v	v	NN
cord-014597-66vd2mdu	1585	29	/	/	SYM
cord-014597-66vd2mdu	1585	30	v	v	NN
cord-014597-66vd2mdu	1585	31	)	)	-RRB-
cord-014597-66vd2mdu	1585	32	solvent	solvent	NN
cord-014597-66vd2mdu	1585	33	system	system	NN
cord-014597-66vd2mdu	1585	34	in	in	IN
cord-014597-66vd2mdu	1585	35	the	the	DT
cord-014597-66vd2mdu	1585	36	first	first	JJ
cord-014597-66vd2mdu	1585	37	dimension	dimension	NN
cord-014597-66vd2mdu	1585	38	and	and	CC
cord-014597-66vd2mdu	1585	39	a	a	DT
cord-014597-66vd2mdu	1585	40	CHCl	CHCl	NNS
cord-014597-66vd2mdu	1585	41	3	3	CD
cord-014597-66vd2mdu	1585	42	-MeOH	-meoh	CD
cord-014597-66vd2mdu	1585	43	-	-	HYPH
cord-014597-66vd2mdu	1585	44	Acetic	acetic	JJ
cord-014597-66vd2mdu	1585	45	acid	acid	NN
cord-014597-66vd2mdu	1585	46	-	-	HYPH
cord-014597-66vd2mdu	1585	47	H	h	NN
cord-014597-66vd2mdu	1585	48	2	2	CD
cord-014597-66vd2mdu	1585	49	O	o	NN
cord-014597-66vd2mdu	1585	50	(	(	-LRB-
cord-014597-66vd2mdu	1585	51	76:9:12:2	76:9:12:2	NNP
cord-014597-66vd2mdu	1585	52	,	,	,
cord-014597-66vd2mdu	1585	53	v	v	NN
cord-014597-66vd2mdu	1585	54	/	/	SYM
cord-014597-66vd2mdu	1585	55	v	v	NN
cord-014597-66vd2mdu	1585	56	/	/	SYM
cord-014597-66vd2mdu	1585	57	v	v	NN
cord-014597-66vd2mdu	1585	58	/	/	SYM
cord-014597-66vd2mdu	1585	59	v	v	NN
cord-014597-66vd2mdu	1585	60	)	)	-RRB-
cord-014597-66vd2mdu	1585	61	solvent	solvent	NN
cord-014597-66vd2mdu	1585	62	system	system	NN
cord-014597-66vd2mdu	1585	63	in	in	IN
cord-014597-66vd2mdu	1585	64	the	the	DT
cord-014597-66vd2mdu	1585	65	second	second	JJ
cord-014597-66vd2mdu	1585	66	dimension	dimension	NN
cord-014597-66vd2mdu	1585	67	.	.	.
cord-014597-66vd2mdu	1586	1	Non	non	JJ
cord-014597-66vd2mdu	1586	2	-	-	JJ
cord-014597-66vd2mdu	1586	3	polar	polar	JJ
cord-014597-66vd2mdu	1586	4	lipids	lipid	NNS
cord-014597-66vd2mdu	1586	5	were	be	VBD
cord-014597-66vd2mdu	1586	6	separated	separate	VBN
cord-014597-66vd2mdu	1586	7	by	by	IN
cord-014597-66vd2mdu	1586	8	1-D	1-d	CD
cord-014597-66vd2mdu	1586	9	HPTLC	HPTLC	NNP
cord-014597-66vd2mdu	1586	10	using	use	VBG
cord-014597-66vd2mdu	1586	11	hexane	hexane	NN
cord-014597-66vd2mdu	1586	12	-	-	HYPH
cord-014597-66vd2mdu	1586	13	diethyl	diethyl	JJ
cord-014597-66vd2mdu	1586	14	ether	ether	NN
cord-014597-66vd2mdu	1586	15	-	-	HYPH
cord-014597-66vd2mdu	1586	16	acetic	acetic	NN
cord-014597-66vd2mdu	1586	17	acid	acid	NN
cord-014597-66vd2mdu	1586	18	.	.	.
cord-014597-66vd2mdu	1587	1	2,7-dichlorofluorescein	2,7-dichlorofluorescein	CD
cord-014597-66vd2mdu	1587	2	dye	dye	NN
cord-014597-66vd2mdu	1587	3	was	be	VBD
cord-014597-66vd2mdu	1587	4	used	use	VBN
cord-014597-66vd2mdu	1587	5	to	to	TO
cord-014597-66vd2mdu	1587	6	visualize	visualize	VB
cord-014597-66vd2mdu	1587	7	both	both	DT
cord-014597-66vd2mdu	1587	8	polar	polar	JJ
cord-014597-66vd2mdu	1587	9	and	and	CC
cord-014597-66vd2mdu	1587	10	non	non	JJ
cord-014597-66vd2mdu	1587	11	-	-	JJ
cord-014597-66vd2mdu	1587	12	polar	polar	JJ
cord-014597-66vd2mdu	1587	13	lipids	lipid	NNS
cord-014597-66vd2mdu	1587	14	.	.	.
cord-014597-66vd2mdu	1588	1	Further	further	JJ
cord-014597-66vd2mdu	1588	2	detailed	detailed	JJ
cord-014597-66vd2mdu	1588	3	analysis	analysis	NN
cord-014597-66vd2mdu	1588	4	was	be	VBD
cord-014597-66vd2mdu	1588	5	performed	perform	VBN
cord-014597-66vd2mdu	1588	6	on	on	IN
cord-014597-66vd2mdu	1588	7	a	a	DT
cord-014597-66vd2mdu	1588	8	QqQ	QqQ	NNP
cord-014597-66vd2mdu	1588	9	mass	mass	NN
cord-014597-66vd2mdu	1588	10	spectrometer	spectrometer	NN
cord-014597-66vd2mdu	1588	11	(	(	-LRB-
cord-014597-66vd2mdu	1588	12	Thermo	Thermo	NNP
cord-014597-66vd2mdu	1588	13	TSQ	TSQ	NNP
cord-014597-66vd2mdu	1588	14	VANTAGE	VANTAGE	NNP
cord-014597-66vd2mdu	1588	15	,	,	,
cord-014597-66vd2mdu	1588	16	San	San	NNP
cord-014597-66vd2mdu	1588	17	Jose	Jose	NNP
cord-014597-66vd2mdu	1588	18	,	,	,
cord-014597-66vd2mdu	1588	19	CA	CA	NNP
cord-014597-66vd2mdu	1588	20	)	)	-RRB-
cord-014597-66vd2mdu	1588	21	using	use	VBG
cord-014597-66vd2mdu	1588	22	negative	negative	JJ
cord-014597-66vd2mdu	1588	23	-	-	HYPH
cord-014597-66vd2mdu	1588	24	ion	ion	NN
cord-014597-66vd2mdu	1588	25	and	and	CC
cord-014597-66vd2mdu	1588	26	positive	positive	JJ
cord-014597-66vd2mdu	1588	27	-	-	HYPH
cord-014597-66vd2mdu	1588	28	ion	ion	NN
cord-014597-66vd2mdu	1588	29	ESI	ESI	NNP
cord-014597-66vd2mdu	1588	30	modes	mode	NNS
cord-014597-66vd2mdu	1588	31	as	as	RB
cord-014597-66vd2mdu	1588	32	well	well	RB
cord-014597-66vd2mdu	1588	33	as	as	IN
cord-014597-66vd2mdu	1588	34	negative	negative	JJ
cord-014597-66vd2mdu	1588	35	-	-	HYPH
cord-014597-66vd2mdu	1588	36	ion	ion	NN
cord-014597-66vd2mdu	1588	37	ESI	ESI	NNP
cord-014597-66vd2mdu	1588	38	mode	mode	NN
cord-014597-66vd2mdu	1588	39	in	in	IN
cord-014597-66vd2mdu	1588	40	the	the	DT
cord-014597-66vd2mdu	1588	41	presence	presence	NN
cord-014597-66vd2mdu	1588	42	of	of	IN
cord-014597-66vd2mdu	1588	43	lithium	lithium	NN
cord-014597-66vd2mdu	1588	44	hydroxide	hydroxide	NN
cord-014597-66vd2mdu	1588	45	.	.	.
cord-014597-66vd2mdu	1589	1	In	in	IN
cord-014597-66vd2mdu	1589	2	this	this	DT
cord-014597-66vd2mdu	1589	3	study	study	NN
cord-014597-66vd2mdu	1589	4	,	,	,
cord-014597-66vd2mdu	1589	5	quantitative	quantitative	JJ
cord-014597-66vd2mdu	1589	6	lipidomics	lipidomic	NNS
cord-014597-66vd2mdu	1589	7	was	be	VBD
cord-014597-66vd2mdu	1589	8	coupled	couple	VBN
cord-014597-66vd2mdu	1589	9	with	with	IN
cord-014597-66vd2mdu	1589	10	transcriptomics	transcriptomic	NNS
cord-014597-66vd2mdu	1589	11	to	to	TO
cord-014597-66vd2mdu	1589	12	further	further	RB
cord-014597-66vd2mdu	1589	13	understand	understand	VB
cord-014597-66vd2mdu	1589	14	the	the	DT
cord-014597-66vd2mdu	1589	15	physiological	physiological	JJ
cord-014597-66vd2mdu	1589	16	pathways	pathway	NNS
cord-014597-66vd2mdu	1589	17	of	of	IN
cord-014597-66vd2mdu	1589	18	HEK	HEK	NNP
cord-014597-66vd2mdu	1589	19	,	,	,
cord-014597-66vd2mdu	1589	20	CHO	CHO	NNP
cord-014597-66vd2mdu	1589	21	-	-	HYPH
cord-014597-66vd2mdu	1589	22	M	M	NNP
cord-014597-66vd2mdu	1589	23	and	and	CC
cord-014597-66vd2mdu	1589	24	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1589	25	cells	cell	NNS
cord-014597-66vd2mdu	1589	26	.	.	.
cord-014597-66vd2mdu	1590	1	Initial	initial	JJ
cord-014597-66vd2mdu	1590	2	HP	HP	NNP
cord-014597-66vd2mdu	1590	3	-	-	HYPH
cord-014597-66vd2mdu	1590	4	TLC	TLC	NNP
cord-014597-66vd2mdu	1590	5	analysis	analysis	NN
cord-014597-66vd2mdu	1590	6	indicated	indicate	VBD
cord-014597-66vd2mdu	1590	7	that	that	IN
cord-014597-66vd2mdu	1590	8	major	major	JJ
cord-014597-66vd2mdu	1590	9	lipids	lipid	NNS
cord-014597-66vd2mdu	1590	10	in	in	IN
cord-014597-66vd2mdu	1590	11	these	these	DT
cord-014597-66vd2mdu	1590	12	industrial	industrial	JJ
cord-014597-66vd2mdu	1590	13	cell	cell	NN
cord-014597-66vd2mdu	1590	14	lines	line	NNS
cord-014597-66vd2mdu	1590	15	were	be	VBD
cord-014597-66vd2mdu	1590	16	PE	PE	NNP
cord-014597-66vd2mdu	1590	17	and	and	CC
cord-014597-66vd2mdu	1590	18	PC	pc	NN
cord-014597-66vd2mdu	1590	19	.	.	.
cord-014597-66vd2mdu	1591	1	Other	other	JJ
cord-014597-66vd2mdu	1591	2	polar	polar	JJ
cord-014597-66vd2mdu	1591	3	lipids	lipid	NNS
cord-014597-66vd2mdu	1591	4	such	such	JJ
cord-014597-66vd2mdu	1591	5	as	as	IN
cord-014597-66vd2mdu	1591	6	PI	PI	NNP
cord-014597-66vd2mdu	1591	7	,	,	,
cord-014597-66vd2mdu	1591	8	PS	PS	NNP
cord-014597-66vd2mdu	1591	9	,	,	,
cord-014597-66vd2mdu	1591	10	PG	PG	NNP
cord-014597-66vd2mdu	1591	11	,	,	,
cord-014597-66vd2mdu	1591	12	PA	PA	NNP
cord-014597-66vd2mdu	1591	13	,	,	,
cord-014597-66vd2mdu	1591	14	and	and	CC
cord-014597-66vd2mdu	1591	15	SM	SM	NNP
cord-014597-66vd2mdu	1591	16	were	be	VBD
cord-014597-66vd2mdu	1591	17	lower	low	JJR
cord-014597-66vd2mdu	1591	18	compared	compare	VBN
cord-014597-66vd2mdu	1591	19	to	to	IN
cord-014597-66vd2mdu	1591	20	PC	pc	NN
cord-014597-66vd2mdu	1591	21	and	and	CC
cord-014597-66vd2mdu	1591	22	PE	PE	NNP
cord-014597-66vd2mdu	1591	23	in	in	IN
cord-014597-66vd2mdu	1591	24	exponential	exponential	JJ
cord-014597-66vd2mdu	1591	25	and	and	CC
cord-014597-66vd2mdu	1591	26	stationary	stationary	JJ
cord-014597-66vd2mdu	1591	27	phases	phase	NNS
cord-014597-66vd2mdu	1591	28	of	of	IN
cord-014597-66vd2mdu	1591	29	each	each	DT
cord-014597-66vd2mdu	1591	30	cell	cell	NN
cord-014597-66vd2mdu	1591	31	line	line	NN
cord-014597-66vd2mdu	1591	32	.	.	.
cord-014597-66vd2mdu	1592	1	Figure	figure	NN
cord-014597-66vd2mdu	1592	2	1	1	CD
cord-014597-66vd2mdu	1592	3	represents	represent	VBZ
cord-014597-66vd2mdu	1592	4	2D	2d	JJ
cord-014597-66vd2mdu	1592	5	HP	HP	NNP
cord-014597-66vd2mdu	1592	6	-	-	HYPH
cord-014597-66vd2mdu	1592	7	TLC	TLC	NNP
cord-014597-66vd2mdu	1592	8	results	result	NNS
cord-014597-66vd2mdu	1592	9	of	of	IN
cord-014597-66vd2mdu	1592	10	HEK	HEK	NNP
cord-014597-66vd2mdu	1592	11	with	with	IN
cord-014597-66vd2mdu	1592	12	the	the	DT
cord-014597-66vd2mdu	1592	13	relative	relative	JJ
cord-014597-66vd2mdu	1592	14	quantitation	quantitation	NN
cord-014597-66vd2mdu	1592	15	of	of	IN
cord-014597-66vd2mdu	1592	16	polar	polar	JJ
cord-014597-66vd2mdu	1592	17	lipids	lipid	NNS
cord-014597-66vd2mdu	1592	18	.	.	.
cord-014597-66vd2mdu	1593	1	In	in	IN
cord-014597-66vd2mdu	1593	2	order	order	NN
cord-014597-66vd2mdu	1593	3	to	to	TO
cord-014597-66vd2mdu	1593	4	investigate	investigate	VB
cord-014597-66vd2mdu	1593	5	the	the	DT
cord-014597-66vd2mdu	1593	6	lipid	lipid	NN
cord-014597-66vd2mdu	1593	7	subgroups	subgroup	NNS
cord-014597-66vd2mdu	1593	8	,	,	,
cord-014597-66vd2mdu	1593	9	shotgun	shotgun	NN
cord-014597-66vd2mdu	1593	10	MS	MS	NNP
cord-014597-66vd2mdu	1593	11	analysis	analysis	NN
cord-014597-66vd2mdu	1593	12	was	be	VBD
cord-014597-66vd2mdu	1593	13	conducted	conduct	VBN
cord-014597-66vd2mdu	1593	14	for	for	IN
cord-014597-66vd2mdu	1593	15	both	both	CC
cord-014597-66vd2mdu	1593	16	exponential	exponential	JJ
cord-014597-66vd2mdu	1593	17	and	and	CC
cord-014597-66vd2mdu	1593	18	stationary	stationary	JJ
cord-014597-66vd2mdu	1593	19	growth	growth	NN
cord-014597-66vd2mdu	1593	20	phases	phase	NNS
cord-014597-66vd2mdu	1593	21	of	of	IN
cord-014597-66vd2mdu	1593	22	the	the	DT
cord-014597-66vd2mdu	1593	23	three	three	CD
cord-014597-66vd2mdu	1593	24	cell	cell	NN
cord-014597-66vd2mdu	1593	25	lines	line	NNS
cord-014597-66vd2mdu	1593	26	.	.	.
cord-014597-66vd2mdu	1594	1	MS	MS	NNP
cord-014597-66vd2mdu	1594	2	analysis	analysis	NN
cord-014597-66vd2mdu	1594	3	indicated	indicate	VBD
cord-014597-66vd2mdu	1594	4	that	that	IN
cord-014597-66vd2mdu	1594	5	lysophosphatidylethanolamine	lysophosphatidylethanolamine	NNP
cord-014597-66vd2mdu	1594	6	(	(	-LRB-
cord-014597-66vd2mdu	1594	7	LPE	LPE	NNP
cord-014597-66vd2mdu	1594	8	)	)	-RRB-
cord-014597-66vd2mdu	1594	9	and	and	CC
cord-014597-66vd2mdu	1594	10	lyso	lyso	NN
cord-014597-66vd2mdu	1594	11	-	-	HYPH
cord-014597-66vd2mdu	1594	12	phosphatidylcholine	phosphatidylcholine	NNP
cord-014597-66vd2mdu	1594	13	(	(	-LRB-
cord-014597-66vd2mdu	1594	14	LPC	LPC	NNP
cord-014597-66vd2mdu	1594	15	)	)	-RRB-
cord-014597-66vd2mdu	1594	16	amounts	amount	NNS
cord-014597-66vd2mdu	1594	17	were	be	VBD
cord-014597-66vd2mdu	1594	18	4	4	CD
cord-014597-66vd2mdu	1594	19	-10	-10	NN
cord-014597-66vd2mdu	1594	20	fold	fold	JJ
cord-014597-66vd2mdu	1594	21	and	and	CC
cord-014597-66vd2mdu	1594	22	2	2	CD
cord-014597-66vd2mdu	1594	23	-	-	SYM
cord-014597-66vd2mdu	1594	24	4	4	CD
cord-014597-66vd2mdu	1594	25	fold	fold	VBP
cord-014597-66vd2mdu	1594	26	higher	higher	RBR
cord-014597-66vd2mdu	1594	27	in	in	IN
cord-014597-66vd2mdu	1594	28	HEK	HEK	NNP
cord-014597-66vd2mdu	1594	29	cells	cell	NNS
cord-014597-66vd2mdu	1594	30	compared	compare	VBN
cord-014597-66vd2mdu	1594	31	to	to	IN
cord-014597-66vd2mdu	1594	32	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1594	33	and	and	CC
cord-014597-66vd2mdu	1594	34	CHO	CHO	NNP
cord-014597-66vd2mdu	1594	35	cell	cell	NN
cord-014597-66vd2mdu	1594	36	lines	line	NNS
cord-014597-66vd2mdu	1594	37	.	.	.
cord-014597-66vd2mdu	1595	1	Sphingomyelin	Sphingomyelin	NNP
cord-014597-66vd2mdu	1595	2	(	(	-LRB-
cord-014597-66vd2mdu	1595	3	SM	SM	NNP
cord-014597-66vd2mdu	1595	4	)	)	-RRB-
cord-014597-66vd2mdu	1595	5	was	be	VBD
cord-014597-66vd2mdu	1595	6	another	another	DT
cord-014597-66vd2mdu	1595	7	lipid	lipid	NN
cord-014597-66vd2mdu	1595	8	subgroup	subgroup	NN
cord-014597-66vd2mdu	1595	9	that	that	WDT
cord-014597-66vd2mdu	1595	10	was	be	VBD
cord-014597-66vd2mdu	1595	11	shown	show	VBN
cord-014597-66vd2mdu	1595	12	to	to	TO
cord-014597-66vd2mdu	1595	13	have	have	VB
cord-014597-66vd2mdu	1595	14	a	a	DT
cord-014597-66vd2mdu	1595	15	major	major	JJ
cord-014597-66vd2mdu	1595	16	difference	difference	NN
cord-014597-66vd2mdu	1595	17	between	between	IN
cord-014597-66vd2mdu	1595	18	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1595	19	and	and	CC
cord-014597-66vd2mdu	1595	20	other	other	JJ
cord-014597-66vd2mdu	1595	21	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1595	22	cell	cell	NN
cord-014597-66vd2mdu	1595	23	lines	line	NNS
cord-014597-66vd2mdu	1595	24	.	.	.
cord-014597-66vd2mdu	1596	1	SM	SM	NNP
cord-014597-66vd2mdu	1596	2	was	be	VBD
cord-014597-66vd2mdu	1596	3	30	30	CD
cord-014597-66vd2mdu	1596	4	-	-	SYM
cord-014597-66vd2mdu	1596	5	65	65	CD
cord-014597-66vd2mdu	1596	6	fold	fold	VB
cord-014597-66vd2mdu	1596	7	lower	lower	RBR
cord-014597-66vd2mdu	1596	8	in	in	IN
cord-014597-66vd2mdu	1596	9	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1596	10	cell	cell	NN
cord-014597-66vd2mdu	1596	11	line	line	NN
cord-014597-66vd2mdu	1596	12	compared	compare	VBN
cord-014597-66vd2mdu	1596	13	to	to	IN
cord-014597-66vd2mdu	1596	14	CHO	CHO	NNP
cord-014597-66vd2mdu	1596	15	and	and	CC
cord-014597-66vd2mdu	1596	16	HEK	HEK	NNP
cord-014597-66vd2mdu	1596	17	.	.	.
cord-014597-66vd2mdu	1597	1	To	to	TO
cord-014597-66vd2mdu	1597	2	understand	understand	VB
cord-014597-66vd2mdu	1597	3	these	these	DT
cord-014597-66vd2mdu	1597	4	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1597	5	differences	difference	NNS
cord-014597-66vd2mdu	1597	6	,	,	,
cord-014597-66vd2mdu	1597	7	transcriptomics	transcriptomic	NNS
cord-014597-66vd2mdu	1597	8	analysis	analysis	NN
cord-014597-66vd2mdu	1597	9	using	use	VBG
cord-014597-66vd2mdu	1597	10	Illumina	Illumina	NNP
cord-014597-66vd2mdu	1597	11	HighSeq	HighSeq	NNP
cord-014597-66vd2mdu	1597	12	and	and	CC
cord-014597-66vd2mdu	1597	13	Gene	Gene	NNP
cord-014597-66vd2mdu	1597	14	Expression	Expression	NNP
cord-014597-66vd2mdu	1597	15	Omnibus	Omnibus	NNP
cord-014597-66vd2mdu	1597	16	was	be	VBD
cord-014597-66vd2mdu	1597	17	conducted	conduct	VBN
cord-014597-66vd2mdu	1597	18	on	on	IN
cord-014597-66vd2mdu	1597	19	these	these	DT
cord-014597-66vd2mdu	1597	20	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1597	21	cells	cell	NNS
cord-014597-66vd2mdu	1597	22	.	.	.
cord-014597-66vd2mdu	1598	1	The	the	DT
cord-014597-66vd2mdu	1598	2	Kyoto	Kyoto	NNP
cord-014597-66vd2mdu	1598	3	Encyclopedia	Encyclopedia	NNP
cord-014597-66vd2mdu	1598	4	of	of	IN
cord-014597-66vd2mdu	1598	5	Genes	Genes	NNPS
cord-014597-66vd2mdu	1598	6	and	and	CC
cord-014597-66vd2mdu	1598	7	Genomes	Genomes	NNPS
cord-014597-66vd2mdu	1598	8	(	(	-LRB-
cord-014597-66vd2mdu	1598	9	KEGG	KEGG	NNP
cord-014597-66vd2mdu	1598	10	)	)	-RRB-
cord-014597-66vd2mdu	1598	11	database	database	NN
cord-014597-66vd2mdu	1598	12	was	be	VBD
cord-014597-66vd2mdu	1598	13	used	use	VBN
cord-014597-66vd2mdu	1598	14	to	to	TO
cord-014597-66vd2mdu	1598	15	map	map	VB
cord-014597-66vd2mdu	1598	16	the	the	DT
cord-014597-66vd2mdu	1598	17	transcriptomics	transcriptomics	NNP
cord-014597-66vd2mdu	1598	18	data	datum	NNS
cord-014597-66vd2mdu	1598	19	to	to	IN
cord-014597-66vd2mdu	1598	20	the	the	DT
cord-014597-66vd2mdu	1598	21	lipid	lipid	NN
cord-014597-66vd2mdu	1598	22	synthetic	synthetic	JJ
cord-014597-66vd2mdu	1598	23	pathways	pathway	NNS
cord-014597-66vd2mdu	1598	24	.	.	.
cord-014597-66vd2mdu	1599	1	Transcriptomics	Transcriptomics	NNP
cord-014597-66vd2mdu	1599	2	data	data	NN
cord-014597-66vd2mdu	1599	3	mapping	mapping	NN
cord-014597-66vd2mdu	1599	4	to	to	IN
cord-014597-66vd2mdu	1599	5	KEGG	KEGG	NNP
cord-014597-66vd2mdu	1599	6	pathways	pathway	NNS
cord-014597-66vd2mdu	1599	7	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	1599	8	that	that	IN
cord-014597-66vd2mdu	1599	9	differences	difference	NNS
cord-014597-66vd2mdu	1599	10	in	in	IN
cord-014597-66vd2mdu	1599	11	LPE	LPE	NNP
cord-014597-66vd2mdu	1599	12	and	and	CC
cord-014597-66vd2mdu	1599	13	LPC	LPC	NNP
cord-014597-66vd2mdu	1599	14	pathways	pathway	NNS
cord-014597-66vd2mdu	1599	15	correlate	correlate	VBP
cord-014597-66vd2mdu	1599	16	with	with	IN
cord-014597-66vd2mdu	1599	17	the	the	DT
cord-014597-66vd2mdu	1599	18	expression	expression	NN
cord-014597-66vd2mdu	1599	19	profiles	profile	NNS
cord-014597-66vd2mdu	1599	20	of	of	IN
cord-014597-66vd2mdu	1599	21	secretory	secretory	JJ
cord-014597-66vd2mdu	1599	22	phospholipase	phospholipase	NN
cord-014597-66vd2mdu	1599	23	A2	A2	NNP
cord-014597-66vd2mdu	1599	24	(	(	-LRB-
cord-014597-66vd2mdu	1599	25	sPLA2	sPLA2	NNP
cord-014597-66vd2mdu	1599	26	)	)	-RRB-
cord-014597-66vd2mdu	1599	27	,	,	,
cord-014597-66vd2mdu	1599	28	lysophospholipid	lysophospholipid	NNP
cord-014597-66vd2mdu	1599	29	acyltransferase	acyltransferase	NNP
cord-014597-66vd2mdu	1599	30	(	(	-LRB-
cord-014597-66vd2mdu	1599	31	LPEAT	LPEAT	NNP
cord-014597-66vd2mdu	1599	32	)	)	-RRB-
cord-014597-66vd2mdu	1599	33	,	,	,
cord-014597-66vd2mdu	1599	34	lysophosphatidylcholine	lysophosphatidylcholine	NNP
cord-014597-66vd2mdu	1599	35	acyltransferase	acyltransferase	NNP
cord-014597-66vd2mdu	1599	36	(	(	-LRB-
cord-014597-66vd2mdu	1599	37	LPCAT	LPCAT	NNP
cord-014597-66vd2mdu	1599	38	)	)	-RRB-
cord-014597-66vd2mdu	1599	39	,	,	,
cord-014597-66vd2mdu	1599	40	and	and	CC
cord-014597-66vd2mdu	1599	41	lysophospholipase	lysophospholipase	NNP
cord-014597-66vd2mdu	1599	42	(	(	-LRB-
cord-014597-66vd2mdu	1599	43	LYPLA	LYPLA	NNP
cord-014597-66vd2mdu	1599	44	)	)	-RRB-
cord-014597-66vd2mdu	1600	1	[	[	-LRB-
cord-014597-66vd2mdu	1600	2	1	1	CD
cord-014597-66vd2mdu	1600	3	]	]	-RRB-
cord-014597-66vd2mdu	1600	4	.	.	.
cord-014597-66vd2mdu	1601	1	The	the	DT
cord-014597-66vd2mdu	1601	2	HP	HP	NNP
cord-014597-66vd2mdu	1601	3	-	-	HYPH
cord-014597-66vd2mdu	1601	4	TLC	TLC	NNP
cord-014597-66vd2mdu	1601	5	and	and	CC
cord-014597-66vd2mdu	1601	6	LC	LC	NNP
cord-014597-66vd2mdu	1601	7	/	/	SYM
cord-014597-66vd2mdu	1601	8	MS	MS	NNP
cord-014597-66vd2mdu	1601	9	findings	finding	NNS
cord-014597-66vd2mdu	1601	10	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	1601	11	that	that	IN
cord-014597-66vd2mdu	1601	12	high	high	JJ
cord-014597-66vd2mdu	1601	13	levels	level	NNS
cord-014597-66vd2mdu	1601	14	of	of	IN
cord-014597-66vd2mdu	1601	15	LPE	LPE	NNP
cord-014597-66vd2mdu	1601	16	and	and	CC
cord-014597-66vd2mdu	1601	17	LPC	LPC	NNP
cord-014597-66vd2mdu	1601	18	existed	exist	VBD
cord-014597-66vd2mdu	1601	19	in	in	IN
cord-014597-66vd2mdu	1601	20	the	the	DT
cord-014597-66vd2mdu	1601	21	HEK	HEK	NNP
cord-014597-66vd2mdu	1601	22	cell	cell	NN
cord-014597-66vd2mdu	1601	23	line	line	NN
cord-014597-66vd2mdu	1601	24	and	and	CC
cord-014597-66vd2mdu	1601	25	low	low	JJ
cord-014597-66vd2mdu	1601	26	levels	level	NNS
cord-014597-66vd2mdu	1601	27	of	of	IN
cord-014597-66vd2mdu	1601	28	SM	SM	NNP
cord-014597-66vd2mdu	1601	29	were	be	VBD
cord-014597-66vd2mdu	1601	30	observed	observe	VBN
cord-014597-66vd2mdu	1601	31	in	in	IN
cord-014597-66vd2mdu	1601	32	the	the	DT
cord-014597-66vd2mdu	1601	33	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1601	34	cell	cell	NN
cord-014597-66vd2mdu	1601	35	line	line	NN
cord-014597-66vd2mdu	1601	36	.	.	.
cord-014597-66vd2mdu	1602	1	Coupling	couple	VBG
cord-014597-66vd2mdu	1602	2	lipidomics	lipidomic	NNS
cord-014597-66vd2mdu	1602	3	with	with	IN
cord-014597-66vd2mdu	1602	4	transcriptomics	transcriptomic	NNS
cord-014597-66vd2mdu	1602	5	provides	provide	VBZ
cord-014597-66vd2mdu	1602	6	us	-PRON-	PRP
cord-014597-66vd2mdu	1602	7	with	with	IN
cord-014597-66vd2mdu	1602	8	an	an	DT
cord-014597-66vd2mdu	1602	9	improved	improved	JJ
cord-014597-66vd2mdu	1602	10	understanding	understanding	NN
cord-014597-66vd2mdu	1602	11	of	of	IN
cord-014597-66vd2mdu	1602	12	the	the	DT
cord-014597-66vd2mdu	1602	13	physiological	physiological	JJ
cord-014597-66vd2mdu	1602	14	differences	difference	NNS
cord-014597-66vd2mdu	1602	15	across	across	IN
cord-014597-66vd2mdu	1602	16	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	1602	17	,	,	,
cord-014597-66vd2mdu	1602	18	CHO	CHO	NNP
cord-014597-66vd2mdu	1602	19	,	,	,
cord-014597-66vd2mdu	1602	20	and	and	CC
cord-014597-66vd2mdu	1602	21	HEK	HEK	NNP
cord-014597-66vd2mdu	1602	22	cell	cell	NN
cord-014597-66vd2mdu	1602	23	lines	line	NNS
cord-014597-66vd2mdu	1602	24	that	that	WDT
cord-014597-66vd2mdu	1602	25	could	could	MD
cord-014597-66vd2mdu	1602	26	be	be	VB
cord-014597-66vd2mdu	1602	27	used	use	VBN
cord-014597-66vd2mdu	1602	28	to	to	TO
cord-014597-66vd2mdu	1602	29	guide	guide	VB
cord-014597-66vd2mdu	1602	30	cell	cell	NN
cord-014597-66vd2mdu	1602	31	engineering	engineering	NN
cord-014597-66vd2mdu	1602	32	efforts	effort	NNS
cord-014597-66vd2mdu	1602	33	with	with	IN
cord-014597-66vd2mdu	1602	34	the	the	DT
cord-014597-66vd2mdu	1602	35	goal	goal	NN
cord-014597-66vd2mdu	1602	36	of	of	IN
cord-014597-66vd2mdu	1602	37	increasing	increase	VBG
cord-014597-66vd2mdu	1602	38	the	the	DT
cord-014597-66vd2mdu	1602	39	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1602	40	protein	protein	NN
cord-014597-66vd2mdu	1602	41	expression	expression	NN
cord-014597-66vd2mdu	1602	42	capabilities	capability	NNS
cord-014597-66vd2mdu	1602	43	of	of	IN
cord-014597-66vd2mdu	1602	44	these	these	DT
cord-014597-66vd2mdu	1602	45	three	three	CD
cord-014597-66vd2mdu	1602	46	cell	cell	NN
cord-014597-66vd2mdu	1602	47	lines	line	NNS
cord-014597-66vd2mdu	1602	48	.	.	.
cord-014597-66vd2mdu	1603	1	Biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	1603	2	are	be	VBP
cord-014597-66vd2mdu	1603	3	a	a	DT
cord-014597-66vd2mdu	1603	4	class	class	NN
cord-014597-66vd2mdu	1603	5	of	of	IN
cord-014597-66vd2mdu	1603	6	biological	biological	JJ
cord-014597-66vd2mdu	1603	7	macromolecules	macromolecule	NNS
cord-014597-66vd2mdu	1603	8	that	that	WDT
cord-014597-66vd2mdu	1603	9	include	include	VBP
cord-014597-66vd2mdu	1603	10	antibodies	antibody	NNS
cord-014597-66vd2mdu	1603	11	and	and	CC
cord-014597-66vd2mdu	1603	12	antibody	antibody	NN
cord-014597-66vd2mdu	1603	13	derivatives	derivative	NNS
cord-014597-66vd2mdu	1603	14	,	,	,
cord-014597-66vd2mdu	1603	15	generally	generally	RB
cord-014597-66vd2mdu	1603	16	produced	produce	VBN
cord-014597-66vd2mdu	1603	17	from	from	IN
cord-014597-66vd2mdu	1603	18	cultured	cultured	JJ
cord-014597-66vd2mdu	1603	19	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1603	20	cell	cell	NN
cord-014597-66vd2mdu	1603	21	lines	line	NNS
cord-014597-66vd2mdu	1603	22	via	via	IN
cord-014597-66vd2mdu	1603	23	secretion	secretion	NN
cord-014597-66vd2mdu	1603	24	directly	directly	RB
cord-014597-66vd2mdu	1603	25	into	into	IN
cord-014597-66vd2mdu	1603	26	the	the	DT
cord-014597-66vd2mdu	1603	27	media	medium	NNS
cord-014597-66vd2mdu	1603	28	.	.	.
cord-014597-66vd2mdu	1604	1	Manufacturing	manufacture	VBG
cord-014597-66vd2mdu	1604	2	at	at	IN
cord-014597-66vd2mdu	1604	3	MedImmune	MedImmune	NNP
cord-014597-66vd2mdu	1604	4	requires	require	VBZ
cord-014597-66vd2mdu	1604	5	the	the	DT
cord-014597-66vd2mdu	1604	6	generation	generation	NN
cord-014597-66vd2mdu	1604	7	of	of	IN
cord-014597-66vd2mdu	1604	8	Chinese	chinese	JJ
cord-014597-66vd2mdu	1604	9	hamster	hamster	NN
cord-014597-66vd2mdu	1604	10	ovary	ovary	NN
cord-014597-66vd2mdu	1604	11	(	(	-LRB-
cord-014597-66vd2mdu	1604	12	CHO	CHO	NNP
cord-014597-66vd2mdu	1604	13	)	)	-RRB-
cord-014597-66vd2mdu	1604	14	clonal	clonal	JJ
cord-014597-66vd2mdu	1604	15	cell	cell	NN
cord-014597-66vd2mdu	1604	16	lines	line	NNS
cord-014597-66vd2mdu	1604	17	capable	capable	JJ
cord-014597-66vd2mdu	1604	18	of	of	IN
cord-014597-66vd2mdu	1604	19	producing	produce	VBG
cord-014597-66vd2mdu	1604	20	the	the	DT
cord-014597-66vd2mdu	1604	21	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	1604	22	product	product	NN
cord-014597-66vd2mdu	1604	23	at	at	IN
cord-014597-66vd2mdu	1604	24	commercially	commercially	RB
cord-014597-66vd2mdu	1604	25	relevant	relevant	JJ
cord-014597-66vd2mdu	1604	26	quantities	quantity	NNS
cord-014597-66vd2mdu	1604	27	with	with	IN
cord-014597-66vd2mdu	1604	28	optimal	optimal	JJ
cord-014597-66vd2mdu	1604	29	product	product	NN
cord-014597-66vd2mdu	1604	30	quality	quality	NN
cord-014597-66vd2mdu	1604	31	.	.	.
cord-014597-66vd2mdu	1605	1	The	the	DT
cord-014597-66vd2mdu	1605	2	isolation	isolation	NN
cord-014597-66vd2mdu	1605	3	of	of	IN
cord-014597-66vd2mdu	1605	4	cell	cell	NN
cord-014597-66vd2mdu	1605	5	clones	clone	NNS
cord-014597-66vd2mdu	1605	6	based	base	VBN
cord-014597-66vd2mdu	1605	7	on	on	IN
cord-014597-66vd2mdu	1605	8	random	random	JJ
cord-014597-66vd2mdu	1605	9	single	single	JJ
cord-014597-66vd2mdu	1605	10	cell	cell	NN
cord-014597-66vd2mdu	1605	11	deposition	deposition	NN
cord-014597-66vd2mdu	1605	12	via	via	IN
cord-014597-66vd2mdu	1605	13	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	1605	14	activated	activate	VBN
cord-014597-66vd2mdu	1605	15	cell	cell	NN
cord-014597-66vd2mdu	1605	16	sorting	sorting	NN
cord-014597-66vd2mdu	1605	17	(	(	-LRB-
cord-014597-66vd2mdu	1605	18	FACS	FACS	NNP
cord-014597-66vd2mdu	1605	19	)	)	-RRB-
cord-014597-66vd2mdu	1605	20	provides	provide	VBZ
cord-014597-66vd2mdu	1605	21	a	a	DT
cord-014597-66vd2mdu	1605	22	heterogeneous	heterogeneous	JJ
cord-014597-66vd2mdu	1605	23	panel	panel	NN
cord-014597-66vd2mdu	1605	24	of	of	IN
cord-014597-66vd2mdu	1605	25	expressers	expresser	NNS
cord-014597-66vd2mdu	1605	26	.	.	.
cord-014597-66vd2mdu	1606	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1606	2	hypothesize	hypothesize	VBP
cord-014597-66vd2mdu	1606	3	that	that	IN
cord-014597-66vd2mdu	1606	4	the	the	DT
cord-014597-66vd2mdu	1606	5	application	application	NN
cord-014597-66vd2mdu	1606	6	of	of	IN
cord-014597-66vd2mdu	1606	7	FACS	FACS	NNP
cord-014597-66vd2mdu	1606	8	to	to	TO
cord-014597-66vd2mdu	1606	9	provide	provide	VB
cord-014597-66vd2mdu	1606	10	an	an	DT
cord-014597-66vd2mdu	1606	11	additional	additional	JJ
cord-014597-66vd2mdu	1606	12	sorting	sorting	NN
cord-014597-66vd2mdu	1606	13	step	step	NN
cord-014597-66vd2mdu	1606	14	based	base	VBN
cord-014597-66vd2mdu	1606	15	on	on	IN
cord-014597-66vd2mdu	1606	16	desirable	desirable	JJ
cord-014597-66vd2mdu	1606	17	cell	cell	NN
cord-014597-66vd2mdu	1606	18	attributes	attribute	NNS
cord-014597-66vd2mdu	1606	19	that	that	WDT
cord-014597-66vd2mdu	1606	20	correlate	correlate	VBP
cord-014597-66vd2mdu	1606	21	with	with	IN
cord-014597-66vd2mdu	1606	22	productivity	productivity	NN
cord-014597-66vd2mdu	1606	23	,	,	,
cord-014597-66vd2mdu	1606	24	product	product	NN
cord-014597-66vd2mdu	1606	25	quality	quality	NN
cord-014597-66vd2mdu	1606	26	or	or	CC
cord-014597-66vd2mdu	1606	27	cell	cell	NN
cord-014597-66vd2mdu	1606	28	growth	growth	NN
cord-014597-66vd2mdu	1606	29	attributes	attribute	NNS
cord-014597-66vd2mdu	1606	30	could	could	MD
cord-014597-66vd2mdu	1606	31	lead	lead	VB
cord-014597-66vd2mdu	1606	32	to	to	IN
cord-014597-66vd2mdu	1606	33	the	the	DT
cord-014597-66vd2mdu	1606	34	isolation	isolation	NN
cord-014597-66vd2mdu	1606	35	of	of	IN
cord-014597-66vd2mdu	1606	36	higher	high	JJR
cord-014597-66vd2mdu	1606	37	producing	produce	VBG
cord-014597-66vd2mdu	1606	38	cell	cell	NN
cord-014597-66vd2mdu	1606	39	lines	line	NNS
cord-014597-66vd2mdu	1606	40	with	with	IN
cord-014597-66vd2mdu	1606	41	enhanced	enhanced	JJ
cord-014597-66vd2mdu	1606	42	product	product	NN
cord-014597-66vd2mdu	1606	43	quality	quality	NN
cord-014597-66vd2mdu	1606	44	attributes	attribute	NNS
cord-014597-66vd2mdu	1606	45	.	.	.
cord-014597-66vd2mdu	1607	1	A	a	DT
cord-014597-66vd2mdu	1607	2	panel	panel	NN
cord-014597-66vd2mdu	1607	3	of	of	IN
cord-014597-66vd2mdu	1607	4	20	20	CD
cord-014597-66vd2mdu	1607	5	cell	cell	NN
cord-014597-66vd2mdu	1607	6	lines	line	NNS
cord-014597-66vd2mdu	1607	7	expressing	express	VBG
cord-014597-66vd2mdu	1607	8	a	a	DT
cord-014597-66vd2mdu	1607	9	model	model	NN
cord-014597-66vd2mdu	1607	10	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1607	11	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1607	12	antibody	antibody	NN
cord-014597-66vd2mdu	1607	13	were	be	VBD
cord-014597-66vd2mdu	1607	14	characterised	characterise	VBN
cord-014597-66vd2mdu	1607	15	in	in	IN
cord-014597-66vd2mdu	1607	16	terms	term	NNS
cord-014597-66vd2mdu	1607	17	of	of	IN
cord-014597-66vd2mdu	1607	18	growth	growth	NN
cord-014597-66vd2mdu	1607	19	,	,	,
cord-014597-66vd2mdu	1607	20	productivity	productivity	NN
cord-014597-66vd2mdu	1607	21	,	,	,
cord-014597-66vd2mdu	1607	22	intracellular	intracellular	JJ
cord-014597-66vd2mdu	1607	23	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1607	24	protein	protein	NN
cord-014597-66vd2mdu	1607	25	and	and	CC
cord-014597-66vd2mdu	1607	26	mRNA	mrna	NN
cord-014597-66vd2mdu	1607	27	amounts	amount	NNS
cord-014597-66vd2mdu	1607	28	.	.	.
cord-014597-66vd2mdu	1608	1	Assays	assay	NNS
cord-014597-66vd2mdu	1608	2	were	be	VBD
cord-014597-66vd2mdu	1608	3	also	also	RB
cord-014597-66vd2mdu	1608	4	developed	develop	VBN
cord-014597-66vd2mdu	1608	5	to	to	TO
cord-014597-66vd2mdu	1608	6	investigate	investigate	VB
cord-014597-66vd2mdu	1608	7	cell	cell	NN
cord-014597-66vd2mdu	1608	8	attributes	attribute	NNS
cord-014597-66vd2mdu	1608	9	using	use	VBG
cord-014597-66vd2mdu	1608	10	the	the	DT
cord-014597-66vd2mdu	1608	11	commercially	commercially	RB
cord-014597-66vd2mdu	1608	12	available	available	JJ
cord-014597-66vd2mdu	1608	13	ImageStream	ImageStream	NNP
cord-014597-66vd2mdu	1608	14	instrument	instrument	NN
cord-014597-66vd2mdu	1608	15	,	,	,
cord-014597-66vd2mdu	1608	16	an	an	DT
cord-014597-66vd2mdu	1608	17	imaging	imaging	NN
cord-014597-66vd2mdu	1608	18	flow	flow	NN
cord-014597-66vd2mdu	1608	19	cytometer	cytometer	NN
cord-014597-66vd2mdu	1608	20	,	,	,
cord-014597-66vd2mdu	1608	21	which	which	WDT
cord-014597-66vd2mdu	1608	22	enables	enable	VBZ
cord-014597-66vd2mdu	1608	23	the	the	DT
cord-014597-66vd2mdu	1608	24	investigation	investigation	NN
cord-014597-66vd2mdu	1608	25	of	of	IN
cord-014597-66vd2mdu	1608	26	cellular	cellular	JJ
cord-014597-66vd2mdu	1608	27	characteristics	characteristic	NNS
cord-014597-66vd2mdu	1608	28	that	that	WDT
cord-014597-66vd2mdu	1608	29	correlate	correlate	VBP
cord-014597-66vd2mdu	1608	30	with	with	IN
cord-014597-66vd2mdu	1608	31	cell	cell	NN
cord-014597-66vd2mdu	1608	32	productivity	productivity	NN
cord-014597-66vd2mdu	1608	33	at	at	IN
cord-014597-66vd2mdu	1608	34	the	the	DT
cord-014597-66vd2mdu	1608	35	single	single	JJ
cord-014597-66vd2mdu	1608	36	cell	cell	NN
cord-014597-66vd2mdu	1608	37	level	level	NN
cord-014597-66vd2mdu	1608	38	.	.	.
cord-014597-66vd2mdu	1609	1	Characterisation	Characterisation	NNP
cord-014597-66vd2mdu	1609	2	revealed	reveal	VBD
cord-014597-66vd2mdu	1609	3	the	the	DT
cord-014597-66vd2mdu	1609	4	cell	cell	NN
cord-014597-66vd2mdu	1609	5	lines	line	NNS
cord-014597-66vd2mdu	1609	6	exhibited	exhibit	VBD
cord-014597-66vd2mdu	1609	7	a	a	DT
cord-014597-66vd2mdu	1609	8	range	range	NN
cord-014597-66vd2mdu	1609	9	of	of	IN
cord-014597-66vd2mdu	1609	10	values	value	NNS
cord-014597-66vd2mdu	1609	11	for	for	IN
cord-014597-66vd2mdu	1609	12	productivity	productivity	NN
cord-014597-66vd2mdu	1609	13	,	,	,
cord-014597-66vd2mdu	1609	14	growth	growth	NN
cord-014597-66vd2mdu	1609	15	,	,	,
cord-014597-66vd2mdu	1609	16	and	and	CC
cord-014597-66vd2mdu	1609	17	intracellular	intracellular	JJ
cord-014597-66vd2mdu	1609	18	(	(	-LRB-
cord-014597-66vd2mdu	1609	19	IC	IC	NNP
cord-014597-66vd2mdu	1609	20	)	)	-RRB-
cord-014597-66vd2mdu	1609	21	antibody	antibody	NN
cord-014597-66vd2mdu	1609	22	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1609	23	and	and	CC
cord-014597-66vd2mdu	1609	24	protein	protein	NN
cord-014597-66vd2mdu	1609	25	expression	expression	NN
cord-014597-66vd2mdu	1609	26	,	,	,
cord-014597-66vd2mdu	1609	27	ideal	ideal	JJ
cord-014597-66vd2mdu	1609	28	for	for	IN
cord-014597-66vd2mdu	1609	29	further	further	JJ
cord-014597-66vd2mdu	1609	30	ImageStream	ImageStream	NNP
cord-014597-66vd2mdu	1609	31	characterisation	characterisation	NN
cord-014597-66vd2mdu	1609	32	.	.	.
cord-014597-66vd2mdu	1610	1	Western	western	JJ
cord-014597-66vd2mdu	1610	2	blot	blot	NN
cord-014597-66vd2mdu	1610	3	and	and	CC
cord-014597-66vd2mdu	1610	4	qRT	qRT	NNP
cord-014597-66vd2mdu	1610	5	-	-	HYPH
cord-014597-66vd2mdu	1610	6	PCR	PCR	NNP
cord-014597-66vd2mdu	1610	7	analysis	analysis	NN
cord-014597-66vd2mdu	1610	8	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	1610	9	that	that	IN
cord-014597-66vd2mdu	1610	10	final	final	JJ
cord-014597-66vd2mdu	1610	11	titre	titre	NN
cord-014597-66vd2mdu	1610	12	correlated	correlate	VBN
cord-014597-66vd2mdu	1610	13	with	with	IN
cord-014597-66vd2mdu	1610	14	both	both	DT
cord-014597-66vd2mdu	1610	15	IC	IC	NNP
cord-014597-66vd2mdu	1610	16	heavy	heavy	JJ
cord-014597-66vd2mdu	1610	17	chain	chain	NN
cord-014597-66vd2mdu	1611	1	(	(	-LRB-
cord-014597-66vd2mdu	1611	2	HC	HC	NNP
cord-014597-66vd2mdu	1611	3	)	)	-RRB-
cord-014597-66vd2mdu	1611	4	protein	protein	NNP
cord-014597-66vd2mdu	1611	5	and	and	CC
cord-014597-66vd2mdu	1611	6	mRNA	mrna	NN
cord-014597-66vd2mdu	1611	7	amounts	amount	NNS
cord-014597-66vd2mdu	1611	8	(	(	-LRB-
cord-014597-66vd2mdu	1611	9	Pearson	Pearson	NNP
cord-014597-66vd2mdu	1611	10	Correlation	Correlation	NNP
cord-014597-66vd2mdu	1611	11	Coefficient	Coefficient	NNP
cord-014597-66vd2mdu	1611	12	(	(	-LRB-
cord-014597-66vd2mdu	1611	13	PCC	PCC	NNP
cord-014597-66vd2mdu	1611	14	)	)	-RRB-
cord-014597-66vd2mdu	1611	15	=	=	SYM
cord-014597-66vd2mdu	1611	16	0.70	0.70	CD
cord-014597-66vd2mdu	1611	17	and	and	CC
cord-014597-66vd2mdu	1611	18	PCC	PCC	NNP
cord-014597-66vd2mdu	1611	19	=	=	SYM
cord-014597-66vd2mdu	1611	20	0.80	0.80	CD
cord-014597-66vd2mdu	1611	21	,	,	,
cord-014597-66vd2mdu	1611	22	respectively	respectively	RB
cord-014597-66vd2mdu	1611	23	)	)	-RRB-
cord-014597-66vd2mdu	1611	24	.	.	.
cord-014597-66vd2mdu	1612	1	To	to	TO
cord-014597-66vd2mdu	1612	2	assess	assess	VB
cord-014597-66vd2mdu	1612	3	productivity	productivity	NN
cord-014597-66vd2mdu	1612	4	at	at	IN
cord-014597-66vd2mdu	1612	5	the	the	DT
cord-014597-66vd2mdu	1612	6	single	single	JJ
cord-014597-66vd2mdu	1612	7	cell	cell	NN
cord-014597-66vd2mdu	1612	8	level	level	NN
cord-014597-66vd2mdu	1612	9	,	,	,
cord-014597-66vd2mdu	1612	10	assays	assay	NNS
cord-014597-66vd2mdu	1612	11	multiplexing	multiplexe	VBG
cord-014597-66vd2mdu	1612	12	IC	IC	NNP
cord-014597-66vd2mdu	1612	13	HC	HC	NNP
cord-014597-66vd2mdu	1612	14	protein	protein	NN
cord-014597-66vd2mdu	1612	15	and	and	CC
cord-014597-66vd2mdu	1612	16	mRNA	mrna	NN
cord-014597-66vd2mdu	1612	17	with	with	IN
cord-014597-66vd2mdu	1612	18	cell	cell	NN
cord-014597-66vd2mdu	1612	19	attributes	attribute	NNS
cord-014597-66vd2mdu	1612	20	were	be	VBD
cord-014597-66vd2mdu	1612	21	therefore	therefore	RB
cord-014597-66vd2mdu	1612	22	developed	develop	VBN
cord-014597-66vd2mdu	1612	23	.	.	.
cord-014597-66vd2mdu	1613	1	Initial	initial	JJ
cord-014597-66vd2mdu	1613	2	assay	assay	NN
cord-014597-66vd2mdu	1613	3	development	development	NN
cord-014597-66vd2mdu	1613	4	focusing	focus	VBG
cord-014597-66vd2mdu	1613	5	on	on	IN
cord-014597-66vd2mdu	1613	6	HC	HC	NNP
cord-014597-66vd2mdu	1613	7	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1613	8	and	and	CC
cord-014597-66vd2mdu	1613	9	protein	protein	NN
cord-014597-66vd2mdu	1613	10	amounts	amount	NNS
cord-014597-66vd2mdu	1613	11	has	have	VBZ
cord-014597-66vd2mdu	1613	12	revealed	reveal	VBN
cord-014597-66vd2mdu	1613	13	interesting	interesting	JJ
cord-014597-66vd2mdu	1613	14	results	result	NNS
cord-014597-66vd2mdu	1613	15	;	;	:
cord-014597-66vd2mdu	1613	16	four	four	CD
cord-014597-66vd2mdu	1613	17	cell	cell	NN
cord-014597-66vd2mdu	1613	18	lines	line	NNS
cord-014597-66vd2mdu	1613	19	displayed	display	VBD
cord-014597-66vd2mdu	1613	20	two	two	CD
cord-014597-66vd2mdu	1613	21	distinct	distinct	JJ
cord-014597-66vd2mdu	1613	22	populations	population	NNS
cord-014597-66vd2mdu	1613	23	,	,	,
cord-014597-66vd2mdu	1613	24	one	one	CD
cord-014597-66vd2mdu	1613	25	producing	produce	VBG
cord-014597-66vd2mdu	1613	26	the	the	DT
cord-014597-66vd2mdu	1613	27	antibody	antibody	NN
cord-014597-66vd2mdu	1613	28	and	and	CC
cord-014597-66vd2mdu	1613	29	another	another	DT
cord-014597-66vd2mdu	1613	30	nonexpressing	nonexpressing	NN
cord-014597-66vd2mdu	1613	31	population	population	NN
cord-014597-66vd2mdu	1613	32	.	.	.
cord-014597-66vd2mdu	1614	1	The	the	DT
cord-014597-66vd2mdu	1614	2	ratio	ratio	NN
cord-014597-66vd2mdu	1614	3	of	of	IN
cord-014597-66vd2mdu	1614	4	these	these	DT
cord-014597-66vd2mdu	1614	5	populations	population	NNS
cord-014597-66vd2mdu	1614	6	varied	varied	JJ
cord-014597-66vd2mdu	1614	7	amongst	amongst	IN
cord-014597-66vd2mdu	1614	8	the	the	DT
cord-014597-66vd2mdu	1614	9	cell	cell	NN
cord-014597-66vd2mdu	1614	10	lines	line	NNS
cord-014597-66vd2mdu	1614	11	.	.	.
cord-014597-66vd2mdu	1615	1	Images	image	NNS
cord-014597-66vd2mdu	1615	2	obtained	obtain	VBN
cord-014597-66vd2mdu	1615	3	from	from	IN
cord-014597-66vd2mdu	1615	4	the	the	DT
cord-014597-66vd2mdu	1615	5	ImageStream	ImageStream	NNP
cord-014597-66vd2mdu	1615	6	have	have	VBP
cord-014597-66vd2mdu	1615	7	shown	show	VBN
cord-014597-66vd2mdu	1615	8	the	the	DT
cord-014597-66vd2mdu	1615	9	cellular	cellular	JJ
cord-014597-66vd2mdu	1615	10	localization	localization	NN
cord-014597-66vd2mdu	1615	11	and	and	CC
cord-014597-66vd2mdu	1615	12	expression	expression	NN
cord-014597-66vd2mdu	1615	13	of	of	IN
cord-014597-66vd2mdu	1615	14	HC	HC	NNP
cord-014597-66vd2mdu	1615	15	and	and	CC
cord-014597-66vd2mdu	1615	16	LC	LC	NNP
cord-014597-66vd2mdu	1615	17	message	message	NN
cord-014597-66vd2mdu	1615	18	and	and	CC
cord-014597-66vd2mdu	1615	19	protein	protein	NN
cord-014597-66vd2mdu	1615	20	(	(	-LRB-
cord-014597-66vd2mdu	1615	21	Fig	Fig	NNP
cord-014597-66vd2mdu	1615	22	.	.	.
cord-014597-66vd2mdu	1615	23	1	1	CD
cord-014597-66vd2mdu	1615	24	)	)	-RRB-
cord-014597-66vd2mdu	1615	25	.	.	.
cord-014597-66vd2mdu	1616	1	For	for	IN
cord-014597-66vd2mdu	1616	2	both	both	DT
cord-014597-66vd2mdu	1616	3	message	message	NN
cord-014597-66vd2mdu	1616	4	and	and	CC
cord-014597-66vd2mdu	1616	5	protein	protein	NN
cord-014597-66vd2mdu	1616	6	,	,	,
cord-014597-66vd2mdu	1616	7	HC	HC	NNP
cord-014597-66vd2mdu	1616	8	and	and	CC
cord-014597-66vd2mdu	1616	9	LC	LC	NNP
cord-014597-66vd2mdu	1616	10	colocalize	colocalize	NN
cord-014597-66vd2mdu	1616	11	in	in	IN
cord-014597-66vd2mdu	1616	12	the	the	DT
cord-014597-66vd2mdu	1616	13	cell	cell	NN
cord-014597-66vd2mdu	1616	14	.	.	.
cord-014597-66vd2mdu	1617	1	Whether	whether	IN
cord-014597-66vd2mdu	1617	2	there	there	EX
cord-014597-66vd2mdu	1617	3	is	be	VBZ
cord-014597-66vd2mdu	1617	4	any	any	DT
cord-014597-66vd2mdu	1617	5	relationship	relationship	NN
cord-014597-66vd2mdu	1617	6	between	between	IN
cord-014597-66vd2mdu	1617	7	IC	IC	NNP
cord-014597-66vd2mdu	1617	8	HC	HC	NNP
cord-014597-66vd2mdu	1617	9	protein	protein	NN
cord-014597-66vd2mdu	1617	10	and	and	CC
cord-014597-66vd2mdu	1617	11	cell	cell	NN
cord-014597-66vd2mdu	1617	12	attributes	attribute	NNS
cord-014597-66vd2mdu	1617	13	at	at	IN
cord-014597-66vd2mdu	1617	14	the	the	DT
cord-014597-66vd2mdu	1617	15	single	single	JJ
cord-014597-66vd2mdu	1617	16	cell	cell	NN
cord-014597-66vd2mdu	1617	17	level	level	NN
cord-014597-66vd2mdu	1617	18	was	be	VBD
cord-014597-66vd2mdu	1617	19	then	then	RB
cord-014597-66vd2mdu	1617	20	also	also	RB
cord-014597-66vd2mdu	1617	21	investigated	investigate	VBN
cord-014597-66vd2mdu	1617	22	,	,	,
cord-014597-66vd2mdu	1617	23	as	as	RB
cord-014597-66vd2mdu	1617	24	well	well	RB
cord-014597-66vd2mdu	1617	25	as	as	IN
cord-014597-66vd2mdu	1617	26	correlations	correlation	NNS
cord-014597-66vd2mdu	1617	27	with	with	IN
cord-014597-66vd2mdu	1617	28	cell	cell	NN
cord-014597-66vd2mdu	1617	29	culture	culture	NN
cord-014597-66vd2mdu	1617	30	parameters	parameter	NNS
cord-014597-66vd2mdu	1617	31	at	at	IN
cord-014597-66vd2mdu	1617	32	the	the	DT
cord-014597-66vd2mdu	1617	33	population	population	NN
cord-014597-66vd2mdu	1617	34	level	level	NN
cord-014597-66vd2mdu	1617	35	.	.	.
cord-014597-66vd2mdu	1618	1	At	at	IN
cord-014597-66vd2mdu	1618	2	the	the	DT
cord-014597-66vd2mdu	1618	3	population	population	NN
cord-014597-66vd2mdu	1618	4	level	level	NN
cord-014597-66vd2mdu	1618	5	,	,	,
cord-014597-66vd2mdu	1618	6	correlations	correlation	NNS
cord-014597-66vd2mdu	1618	7	were	be	VBD
cord-014597-66vd2mdu	1618	8	found	find	VBN
cord-014597-66vd2mdu	1618	9	between	between	IN
cord-014597-66vd2mdu	1618	10	titre	titre	NN
cord-014597-66vd2mdu	1618	11	and	and	CC
cord-014597-66vd2mdu	1618	12	IC	IC	NNP
cord-014597-66vd2mdu	1618	13	HC	HC	NNP
cord-014597-66vd2mdu	1618	14	protein	protein	NN
cord-014597-66vd2mdu	1618	15	and	and	CC
cord-014597-66vd2mdu	1618	16	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1618	17	(	(	-LRB-
cord-014597-66vd2mdu	1618	18	PCC	PCC	NNP
cord-014597-66vd2mdu	1618	19	=	=	SYM
cord-014597-66vd2mdu	1618	20	0.84	0.84	CD
cord-014597-66vd2mdu	1618	21	and	and	CC
cord-014597-66vd2mdu	1618	22	PCC	PCC	NNP
cord-014597-66vd2mdu	1618	23	=	=	SYM
cord-014597-66vd2mdu	1618	24	0.79	0.79	CD
cord-014597-66vd2mdu	1618	25	,	,	,
cord-014597-66vd2mdu	1618	26	respectively	respectively	RB
cord-014597-66vd2mdu	1618	27	)	)	-RRB-
cord-014597-66vd2mdu	1618	28	confirming	confirm	VBG
cord-014597-66vd2mdu	1618	29	the	the	DT
cord-014597-66vd2mdu	1618	30	data	datum	NNS
cord-014597-66vd2mdu	1618	31	obtained	obtain	VBN
cord-014597-66vd2mdu	1618	32	by	by	IN
cord-014597-66vd2mdu	1618	33	western	western	JJ
cord-014597-66vd2mdu	1618	34	blot	blot	NN
cord-014597-66vd2mdu	1618	35	and	and	CC
cord-014597-66vd2mdu	1618	36	qRT	qRT	NNP
cord-014597-66vd2mdu	1618	37	-	-	HYPH
cord-014597-66vd2mdu	1618	38	PCR	PCR	NNP
cord-014597-66vd2mdu	1618	39	analysis	analysis	NN
cord-014597-66vd2mdu	1618	40	.	.	.
cord-014597-66vd2mdu	1619	1	A	a	DT
cord-014597-66vd2mdu	1619	2	panel	panel	NN
cord-014597-66vd2mdu	1619	3	of	of	IN
cord-014597-66vd2mdu	1619	4	20	20	CD
cord-014597-66vd2mdu	1619	5	cell	cell	NN
cord-014597-66vd2mdu	1619	6	lines	line	NNS
cord-014597-66vd2mdu	1619	7	has	have	VBZ
cord-014597-66vd2mdu	1619	8	been	be	VBN
cord-014597-66vd2mdu	1619	9	characterised	characterise	VBN
cord-014597-66vd2mdu	1619	10	at	at	IN
cord-014597-66vd2mdu	1619	11	the	the	DT
cord-014597-66vd2mdu	1619	12	population	population	NN
cord-014597-66vd2mdu	1619	13	level	level	NN
cord-014597-66vd2mdu	1619	14	and	and	CC
cord-014597-66vd2mdu	1619	15	show	show	VBP
cord-014597-66vd2mdu	1619	16	a	a	DT
cord-014597-66vd2mdu	1619	17	wide	wide	JJ
cord-014597-66vd2mdu	1619	18	range	range	NN
cord-014597-66vd2mdu	1619	19	of	of	IN
cord-014597-66vd2mdu	1619	20	antibody	antibody	NN
cord-014597-66vd2mdu	1619	21	expression	expression	NN
cord-014597-66vd2mdu	1619	22	profiles	profile	NNS
cord-014597-66vd2mdu	1619	23	at	at	IN
cord-014597-66vd2mdu	1619	24	both	both	CC
cord-014597-66vd2mdu	1619	25	the	the	DT
cord-014597-66vd2mdu	1619	26	mRNA	mrna	NN
cord-014597-66vd2mdu	1619	27	and	and	CC
cord-014597-66vd2mdu	1619	28	protein	protein	NN
cord-014597-66vd2mdu	1619	29	level	level	NN
cord-014597-66vd2mdu	1619	30	.	.	.
cord-014597-66vd2mdu	1620	1	In	in	IN
cord-014597-66vd2mdu	1620	2	parallel	parallel	NN
cord-014597-66vd2mdu	1620	3	,	,	,
cord-014597-66vd2mdu	1620	4	assays	assay	NNS
cord-014597-66vd2mdu	1620	5	have	have	VBP
cord-014597-66vd2mdu	1620	6	been	be	VBN
cord-014597-66vd2mdu	1620	7	developed	develop	VBN
cord-014597-66vd2mdu	1620	8	for	for	IN
cord-014597-66vd2mdu	1620	9	the	the	DT
cord-014597-66vd2mdu	1620	10	ImageStream	ImageStream	NNP
cord-014597-66vd2mdu	1620	11	to	to	TO
cord-014597-66vd2mdu	1620	12	measure	measure	VB
cord-014597-66vd2mdu	1620	13	HC	HC	NNP
cord-014597-66vd2mdu	1620	14	and	and	CC
cord-014597-66vd2mdu	1620	15	LC	LC	NNP
cord-014597-66vd2mdu	1620	16	message	message	NN
cord-014597-66vd2mdu	1620	17	and	and	CC
cord-014597-66vd2mdu	1620	18	protein	protein	NN
cord-014597-66vd2mdu	1620	19	amounts	amount	NNS
cord-014597-66vd2mdu	1620	20	at	at	IN
cord-014597-66vd2mdu	1620	21	the	the	DT
cord-014597-66vd2mdu	1620	22	single	single	JJ
cord-014597-66vd2mdu	1620	23	cell	cell	NN
cord-014597-66vd2mdu	1620	24	level	level	NN
cord-014597-66vd2mdu	1620	25	.	.	.
cord-014597-66vd2mdu	1621	1	Protein	protein	NN
cord-014597-66vd2mdu	1621	2	and	and	CC
cord-014597-66vd2mdu	1621	3	message	message	NN
cord-014597-66vd2mdu	1621	4	quantification	quantification	NN
cord-014597-66vd2mdu	1621	5	with	with	IN
cord-014597-66vd2mdu	1621	6	the	the	DT
cord-014597-66vd2mdu	1621	7	ImageStream	ImageStream	NNP
cord-014597-66vd2mdu	1621	8	are	be	VBP
cord-014597-66vd2mdu	1621	9	consistent	consistent	JJ
cord-014597-66vd2mdu	1621	10	with	with	IN
cord-014597-66vd2mdu	1621	11	more	more	JJR
cord-014597-66vd2mdu	1621	12	traditional	traditional	JJ
cord-014597-66vd2mdu	1621	13	approaches	approach	NNS
cord-014597-66vd2mdu	1621	14	,	,	,
cord-014597-66vd2mdu	1621	15	such	such	JJ
cord-014597-66vd2mdu	1621	16	as	as	IN
cord-014597-66vd2mdu	1621	17	western	western	JJ
cord-014597-66vd2mdu	1621	18	blots	blot	NNS
cord-014597-66vd2mdu	1621	19	and	and	CC
cord-014597-66vd2mdu	1621	20	qRT	qRT	NNP
cord-014597-66vd2mdu	1621	21	-	-	HYPH
cord-014597-66vd2mdu	1621	22	PCR	PCR	NNP
cord-014597-66vd2mdu	1621	23	,	,	,
cord-014597-66vd2mdu	1621	24	that	that	WDT
cord-014597-66vd2mdu	1621	25	operate	operate	VBP
cord-014597-66vd2mdu	1621	26	at	at	IN
cord-014597-66vd2mdu	1621	27	the	the	DT
cord-014597-66vd2mdu	1621	28	population	population	NN
cord-014597-66vd2mdu	1621	29	level	level	NN
cord-014597-66vd2mdu	1621	30	.	.	.
cord-014597-66vd2mdu	1622	1	The	the	DT
cord-014597-66vd2mdu	1622	2	developed	develop	VBN
cord-014597-66vd2mdu	1622	3	assays	assay	NNS
cord-014597-66vd2mdu	1622	4	are	be	VBP
cord-014597-66vd2mdu	1622	5	now	now	RB
cord-014597-66vd2mdu	1622	6	being	be	VBG
cord-014597-66vd2mdu	1622	7	used	use	VBN
cord-014597-66vd2mdu	1622	8	to	to	TO
cord-014597-66vd2mdu	1622	9	investigate	investigate	VB
cord-014597-66vd2mdu	1622	10	single	single	JJ
cord-014597-66vd2mdu	1622	11	cell	cell	NN
cord-014597-66vd2mdu	1622	12	productivity	productivity	NN
cord-014597-66vd2mdu	1622	13	attributes	attribute	NNS
cord-014597-66vd2mdu	1622	14	and	and	CC
cord-014597-66vd2mdu	1622	15	for	for	IN
cord-014597-66vd2mdu	1622	16	the	the	DT
cord-014597-66vd2mdu	1622	17	isolation	isolation	NN
cord-014597-66vd2mdu	1622	18	of	of	IN
cord-014597-66vd2mdu	1622	19	more	more	JJR
cord-014597-66vd2mdu	1622	20	productive	productive	JJ
cord-014597-66vd2mdu	1622	21	clones	clone	NNS
cord-014597-66vd2mdu	1622	22	.	.	.
cord-014597-66vd2mdu	1623	1	Background	Background	NNP
cord-014597-66vd2mdu	1623	2	Productivity	Productivity	NNP
cord-014597-66vd2mdu	1623	3	and	and	CC
cord-014597-66vd2mdu	1623	4	stability	stability	NN
cord-014597-66vd2mdu	1623	5	are	be	VBP
cord-014597-66vd2mdu	1623	6	key	key	JJ
cord-014597-66vd2mdu	1623	7	factors	factor	NNS
cord-014597-66vd2mdu	1623	8	for	for	IN
cord-014597-66vd2mdu	1623	9	the	the	DT
cord-014597-66vd2mdu	1623	10	selection	selection	NN
cord-014597-66vd2mdu	1623	11	of	of	IN
cord-014597-66vd2mdu	1623	12	cell	cell	NN
cord-014597-66vd2mdu	1623	13	line	line	NN
cord-014597-66vd2mdu	1623	14	in	in	IN
cord-014597-66vd2mdu	1623	15	protein	protein	NN
cord-014597-66vd2mdu	1623	16	drugs	drug	NNS
cord-014597-66vd2mdu	1623	17	production	production	NN
cord-014597-66vd2mdu	1623	18	.	.	.
cord-014597-66vd2mdu	1624	1	Large	large	JJ
cord-014597-66vd2mdu	1624	2	amount	amount	NN
cord-014597-66vd2mdu	1624	3	of	of	IN
cord-014597-66vd2mdu	1624	4	target	target	NN
cord-014597-66vd2mdu	1624	5	gene	gene	NN
cord-014597-66vd2mdu	1624	6	integrated	integrate	VBN
cord-014597-66vd2mdu	1624	7	in	in	IN
cord-014597-66vd2mdu	1624	8	cell	cell	NN
cord-014597-66vd2mdu	1624	9	genome	genome	NN
cord-014597-66vd2mdu	1624	10	could	could	MD
cord-014597-66vd2mdu	1624	11	lead	lead	VB
cord-014597-66vd2mdu	1624	12	to	to	IN
cord-014597-66vd2mdu	1624	13	the	the	DT
cord-014597-66vd2mdu	1624	14	instability	instability	NN
cord-014597-66vd2mdu	1624	15	of	of	IN
cord-014597-66vd2mdu	1624	16	production	production	NN
cord-014597-66vd2mdu	1624	17	.	.	.
cord-014597-66vd2mdu	1625	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1625	2	,	,	,
cord-014597-66vd2mdu	1625	3	cells	cell	NNS
cord-014597-66vd2mdu	1625	4	with	with	IN
cord-014597-66vd2mdu	1625	5	low	low	JJ
cord-014597-66vd2mdu	1625	6	copies	copy	NNS
cord-014597-66vd2mdu	1625	7	of	of	IN
cord-014597-66vd2mdu	1625	8	target	target	NN
cord-014597-66vd2mdu	1625	9	gene	gene	NN
cord-014597-66vd2mdu	1625	10	integrated	integrate	VBN
cord-014597-66vd2mdu	1625	11	in	in	IN
cord-014597-66vd2mdu	1625	12	high	high	JJ
cord-014597-66vd2mdu	1625	13	yield	yield	NN
cord-014597-66vd2mdu	1625	14	sites	site	NNS
cord-014597-66vd2mdu	1625	15	could	could	MD
cord-014597-66vd2mdu	1625	16	be	be	VB
cord-014597-66vd2mdu	1625	17	an	an	DT
cord-014597-66vd2mdu	1625	18	ideal	ideal	JJ
cord-014597-66vd2mdu	1625	19	production	production	NN
cord-014597-66vd2mdu	1625	20	cells	cell	NNS
cord-014597-66vd2mdu	1625	21	for	for	IN
cord-014597-66vd2mdu	1625	22	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1625	23	.	.	.
cord-014597-66vd2mdu	1626	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1626	2	has	have	VBZ
cord-014597-66vd2mdu	1626	3	been	be	VBN
cord-014597-66vd2mdu	1626	4	known	know	VBN
cord-014597-66vd2mdu	1626	5	that	that	IN
cord-014597-66vd2mdu	1626	6	the	the	DT
cord-014597-66vd2mdu	1626	7	transposon	transposon	NN
cord-014597-66vd2mdu	1626	8	system	system	NN
cord-014597-66vd2mdu	1626	9	can	can	MD
cord-014597-66vd2mdu	1626	10	control	control	VB
cord-014597-66vd2mdu	1626	11	the	the	DT
cord-014597-66vd2mdu	1626	12	integrated	integrate	VBN
cord-014597-66vd2mdu	1626	13	copy	copy	NN
cord-014597-66vd2mdu	1626	14	number	number	NN
cord-014597-66vd2mdu	1626	15	of	of	IN
cord-014597-66vd2mdu	1626	16	target	target	NN
cord-014597-66vd2mdu	1626	17	gene	gene	NN
cord-014597-66vd2mdu	1626	18	and	and	CC
cord-014597-66vd2mdu	1626	19	can	can	MD
cord-014597-66vd2mdu	1626	20	generate	generate	VB
cord-014597-66vd2mdu	1626	21	high	high	JJ
cord-014597-66vd2mdu	1626	22	yield	yield	NN
cord-014597-66vd2mdu	1626	23	producing	produce	VBG
cord-014597-66vd2mdu	1626	24	cells	cell	NNS
cord-014597-66vd2mdu	1626	25	,	,	,
cord-014597-66vd2mdu	1626	26	it	-PRON-	PRP
cord-014597-66vd2mdu	1626	27	could	could	MD
cord-014597-66vd2mdu	1626	28	be	be	VB
cord-014597-66vd2mdu	1626	29	a	a	DT
cord-014597-66vd2mdu	1626	30	great	great	JJ
cord-014597-66vd2mdu	1626	31	approach	approach	NN
cord-014597-66vd2mdu	1626	32	to	to	TO
cord-014597-66vd2mdu	1626	33	generate	generate	VB
cord-014597-66vd2mdu	1626	34	stable	stable	JJ
cord-014597-66vd2mdu	1626	35	high	high	JJ
cord-014597-66vd2mdu	1626	36	yield	yield	NN
cord-014597-66vd2mdu	1626	37	producing	produce	VBG
cord-014597-66vd2mdu	1626	38	cell	cell	NN
cord-014597-66vd2mdu	1626	39	lines	line	NNS
cord-014597-66vd2mdu	1626	40	carrying	carry	VBG
cord-014597-66vd2mdu	1626	41	low	low	JJ
cord-014597-66vd2mdu	1626	42	copies	copy	NNS
cord-014597-66vd2mdu	1626	43	of	of	IN
cord-014597-66vd2mdu	1626	44	target	target	NN
cord-014597-66vd2mdu	1626	45	gene	gene	NN
cord-014597-66vd2mdu	1626	46	through	through	IN
cord-014597-66vd2mdu	1626	47	transposon	transposon	NN
cord-014597-66vd2mdu	1626	48	system	system	NN
cord-014597-66vd2mdu	1626	49	.	.	.
cord-014597-66vd2mdu	1627	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1627	2	intended	intend	VBD
cord-014597-66vd2mdu	1627	3	to	to	TO
cord-014597-66vd2mdu	1627	4	develop	develop	VB
cord-014597-66vd2mdu	1627	5	a	a	DT
cord-014597-66vd2mdu	1627	6	platform	platform	NN
cord-014597-66vd2mdu	1627	7	to	to	TO
cord-014597-66vd2mdu	1627	8	generate	generate	VB
cord-014597-66vd2mdu	1627	9	high	high	JJ
cord-014597-66vd2mdu	1627	10	yield	yield	NN
cord-014597-66vd2mdu	1627	11	producing	produce	VBG
cord-014597-66vd2mdu	1627	12	cell	cell	NN
cord-014597-66vd2mdu	1627	13	lines	line	NNS
cord-014597-66vd2mdu	1627	14	carrying	carry	VBG
cord-014597-66vd2mdu	1627	15	1	1	CD
cord-014597-66vd2mdu	1627	16	-	-	SYM
cord-014597-66vd2mdu	1627	17	2	2	CD
cord-014597-66vd2mdu	1627	18	copy	copy	NN
cord-014597-66vd2mdu	1627	19	of	of	IN
cord-014597-66vd2mdu	1627	20	the	the	DT
cord-014597-66vd2mdu	1627	21	integrated	integrated	JJ
cord-014597-66vd2mdu	1627	22	target	target	NN
cord-014597-66vd2mdu	1627	23	gene	gene	NN
cord-014597-66vd2mdu	1627	24	using	use	VBG
cord-014597-66vd2mdu	1627	25	transposon	transposon	NN
cord-014597-66vd2mdu	1627	26	system	system	NN
cord-014597-66vd2mdu	1627	27	.	.	.
cord-014597-66vd2mdu	1628	1	Two	two	CD
cord-014597-66vd2mdu	1628	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1628	3	cell	cell	NN
cord-014597-66vd2mdu	1628	4	lines	line	NNS
cord-014597-66vd2mdu	1628	5	,	,	,
cord-014597-66vd2mdu	1628	6	CHO	CHO	NNP
cord-014597-66vd2mdu	1628	7	-	-	HYPH
cord-014597-66vd2mdu	1628	8	S	S	NNP
cord-014597-66vd2mdu	1628	9	cells	cell	NNS
cord-014597-66vd2mdu	1628	10	and	and	CC
cord-014597-66vd2mdu	1628	11	DXB11	DXB11	NNP
cord-014597-66vd2mdu	1628	12	cells	cell	NNS
cord-014597-66vd2mdu	1628	13	,	,	,
cord-014597-66vd2mdu	1628	14	have	have	VBP
cord-014597-66vd2mdu	1628	15	been	be	VBN
cord-014597-66vd2mdu	1628	16	applied	apply	VBN
cord-014597-66vd2mdu	1628	17	.	.	.
cord-014597-66vd2mdu	1629	1	Cells	cell	NNS
cord-014597-66vd2mdu	1629	2	were	be	VBD
cord-014597-66vd2mdu	1629	3	co	co	VBN
cord-014597-66vd2mdu	1629	4	-	-	VBN
cord-014597-66vd2mdu	1629	5	transfected	transfecte	VBN
cord-014597-66vd2mdu	1629	6	with	with	IN
cord-014597-66vd2mdu	1629	7	transposon	transposon	NN
cord-014597-66vd2mdu	1629	8	and	and	CC
cord-014597-66vd2mdu	1629	9	target	target	NN
cord-014597-66vd2mdu	1629	10	gene	gene	NN
cord-014597-66vd2mdu	1629	11	expression	expression	NN
cord-014597-66vd2mdu	1629	12	plasmids	plasmid	NNS
cord-014597-66vd2mdu	1629	13	.	.	.
cord-014597-66vd2mdu	1630	1	After	after	IN
cord-014597-66vd2mdu	1630	2	drug	drug	NN
cord-014597-66vd2mdu	1630	3	selection	selection	NN
cord-014597-66vd2mdu	1630	4	,	,	,
cord-014597-66vd2mdu	1630	5	the	the	DT
cord-014597-66vd2mdu	1630	6	cell	cell	NN
cord-014597-66vd2mdu	1630	7	pool	pool	NN
cord-014597-66vd2mdu	1630	8	with	with	IN
cord-014597-66vd2mdu	1630	9	highest	high	JJS
cord-014597-66vd2mdu	1630	10	productivity	productivity	NN
cord-014597-66vd2mdu	1630	11	per	per	IN
cord-014597-66vd2mdu	1630	12	target	target	NN
cord-014597-66vd2mdu	1630	13	gene	gene	NN
cord-014597-66vd2mdu	1630	14	copy	copy	NN
cord-014597-66vd2mdu	1630	15	was	be	VBD
cord-014597-66vd2mdu	1630	16	applied	apply	VBN
cord-014597-66vd2mdu	1630	17	to	to	IN
cord-014597-66vd2mdu	1630	18	single	single	JJ
cord-014597-66vd2mdu	1630	19	cell	cell	NN
cord-014597-66vd2mdu	1630	20	cloning	cloning	NN
cord-014597-66vd2mdu	1630	21	.	.	.
cord-014597-66vd2mdu	1631	1	The	the	DT
cord-014597-66vd2mdu	1631	2	productivity	productivity	NN
cord-014597-66vd2mdu	1631	3	and	and	CC
cord-014597-66vd2mdu	1631	4	copy	copy	NN
cord-014597-66vd2mdu	1631	5	number	number	NN
cord-014597-66vd2mdu	1631	6	of	of	IN
cord-014597-66vd2mdu	1631	7	cell	cell	NN
cord-014597-66vd2mdu	1631	8	clones	clone	NNS
cord-014597-66vd2mdu	1631	9	were	be	VBD
cord-014597-66vd2mdu	1631	10	determined	determine	VBN
cord-014597-66vd2mdu	1631	11	,	,	,
cord-014597-66vd2mdu	1631	12	and	and	CC
cord-014597-66vd2mdu	1631	13	the	the	DT
cord-014597-66vd2mdu	1631	14	stability	stability	NN
cord-014597-66vd2mdu	1631	15	of	of	IN
cord-014597-66vd2mdu	1631	16	cell	cell	NN
cord-014597-66vd2mdu	1631	17	clones	clone	NNS
cord-014597-66vd2mdu	1631	18	was	be	VBD
cord-014597-66vd2mdu	1631	19	analysed	analyse	VBN
cord-014597-66vd2mdu	1631	20	after	after	IN
cord-014597-66vd2mdu	1631	21	culture	culture	NN
cord-014597-66vd2mdu	1631	22	of	of	IN
cord-014597-66vd2mdu	1631	23	about	about	RB
cord-014597-66vd2mdu	1631	24	60	60	CD
cord-014597-66vd2mdu	1631	25	generations	generation	NNS
cord-014597-66vd2mdu	1631	26	.	.	.
cord-014597-66vd2mdu	1632	1	In	in	IN
cord-014597-66vd2mdu	1632	2	the	the	DT
cord-014597-66vd2mdu	1632	3	stable	stable	JJ
cord-014597-66vd2mdu	1632	4	pools	pool	NNS
cord-014597-66vd2mdu	1632	5	of	of	IN
cord-014597-66vd2mdu	1632	6	CHO	CHO	NNP
cord-014597-66vd2mdu	1632	7	-	-	HYPH
cord-014597-66vd2mdu	1632	8	S	S	NNP
cord-014597-66vd2mdu	1632	9	and	and	CC
cord-014597-66vd2mdu	1632	10	DXB11	DXB11	NNP
cord-014597-66vd2mdu	1632	11	cells	cell	NNS
cord-014597-66vd2mdu	1632	12	,	,	,
cord-014597-66vd2mdu	1632	13	the	the	DT
cord-014597-66vd2mdu	1632	14	productivities	productivity	NNS
cord-014597-66vd2mdu	1632	15	per	per	IN
cord-014597-66vd2mdu	1632	16	integrated	integrate	VBN
cord-014597-66vd2mdu	1632	17	target	target	NN
cord-014597-66vd2mdu	1632	18	gene	gene	NN
cord-014597-66vd2mdu	1632	19	copy	copy	NN
cord-014597-66vd2mdu	1632	20	were	be	VBD
cord-014597-66vd2mdu	1632	21	about	about	RB
cord-014597-66vd2mdu	1632	22	11	11	CD
cord-014597-66vd2mdu	1632	23	-	-	SYM
cord-014597-66vd2mdu	1632	24	13	13	CD
cord-014597-66vd2mdu	1632	25	mg	mg	NNP
cord-014597-66vd2mdu	1632	26	/	/	SYM
cord-014597-66vd2mdu	1632	27	L	L	NNP
cord-014597-66vd2mdu	1632	28	/	/	SYM
cord-014597-66vd2mdu	1632	29	copy	copy	NN
cord-014597-66vd2mdu	1632	30	and	and	CC
cord-014597-66vd2mdu	1632	31	68	68	CD
cord-014597-66vd2mdu	1632	32	-	-	SYM
cord-014597-66vd2mdu	1632	33	75	75	CD
cord-014597-66vd2mdu	1632	34	mg	mg	NNP
cord-014597-66vd2mdu	1632	35	/	/	SYM
cord-014597-66vd2mdu	1632	36	L	L	NNP
cord-014597-66vd2mdu	1632	37	/	/	SYM
cord-014597-66vd2mdu	1632	38	copy	copy	VBP
cord-014597-66vd2mdu	1632	39	in	in	IN
cord-014597-66vd2mdu	1632	40	a	a	DT
cord-014597-66vd2mdu	1632	41	batch	batch	NN
cord-014597-66vd2mdu	1632	42	culture	culture	NN
cord-014597-66vd2mdu	1632	43	,	,	,
cord-014597-66vd2mdu	1632	44	respectively	respectively	RB
cord-014597-66vd2mdu	1632	45	.	.	.
cord-014597-66vd2mdu	1633	1	After	after	IN
cord-014597-66vd2mdu	1633	2	single	single	JJ
cord-014597-66vd2mdu	1633	3	cell	cell	NN
cord-014597-66vd2mdu	1633	4	cloning	cloning	NN
cord-014597-66vd2mdu	1633	5	,	,	,
cord-014597-66vd2mdu	1633	6	the	the	DT
cord-014597-66vd2mdu	1633	7	integrated	integrated	JJ
cord-014597-66vd2mdu	1633	8	copy	copy	NN
cord-014597-66vd2mdu	1633	9	numbers	number	NNS
cord-014597-66vd2mdu	1633	10	in	in	IN
cord-014597-66vd2mdu	1633	11	most	most	JJS
cord-014597-66vd2mdu	1633	12	cell	cell	NN
cord-014597-66vd2mdu	1633	13	clones	clone	NNS
cord-014597-66vd2mdu	1633	14	were	be	VBD
cord-014597-66vd2mdu	1633	15	less	less	JJR
cord-014597-66vd2mdu	1633	16	than	than	IN
cord-014597-66vd2mdu	1633	17	three	three	CD
cord-014597-66vd2mdu	1633	18	copies	copy	NNS
cord-014597-66vd2mdu	1633	19	per	per	IN
cord-014597-66vd2mdu	1633	20	cell	cell	NN
cord-014597-66vd2mdu	1633	21	.	.	.
cord-014597-66vd2mdu	1634	1	In	in	IN
cord-014597-66vd2mdu	1634	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1634	3	-	-	HYPH
cord-014597-66vd2mdu	1634	4	S	S	NNP
cord-014597-66vd2mdu	1634	5	and	and	CC
cord-014597-66vd2mdu	1634	6	DXB11	DXB11	NNP
cord-014597-66vd2mdu	1634	7	cell	cell	NN
cord-014597-66vd2mdu	1634	8	clones	clone	NNS
cord-014597-66vd2mdu	1634	9	,	,	,
cord-014597-66vd2mdu	1634	10	the	the	DT
cord-014597-66vd2mdu	1634	11	productivities	productivity	NNS
cord-014597-66vd2mdu	1634	12	per	per	IN
cord-014597-66vd2mdu	1634	13	integrated	integrate	VBN
cord-014597-66vd2mdu	1634	14	target	target	NN
cord-014597-66vd2mdu	1634	15	gene	gene	NN
cord-014597-66vd2mdu	1634	16	copy	copy	NN
cord-014597-66vd2mdu	1634	17	were	be	VBD
cord-014597-66vd2mdu	1634	18	20	20	CD
cord-014597-66vd2mdu	1634	19	-	-	SYM
cord-014597-66vd2mdu	1634	20	60	60	CD
cord-014597-66vd2mdu	1634	21	mg	mg	NNP
cord-014597-66vd2mdu	1634	22	/	/	SYM
cord-014597-66vd2mdu	1634	23	L	L	NNP
cord-014597-66vd2mdu	1634	24	/	/	SYM
cord-014597-66vd2mdu	1634	25	copy	copy	NN
cord-014597-66vd2mdu	1634	26	and	and	CC
cord-014597-66vd2mdu	1634	27	60	60	CD
cord-014597-66vd2mdu	1634	28	-	-	SYM
cord-014597-66vd2mdu	1634	29	150	150	CD
cord-014597-66vd2mdu	1634	30	mg	mg	NNP
cord-014597-66vd2mdu	1634	31	/	/	SYM
cord-014597-66vd2mdu	1634	32	L	L	NNP
cord-014597-66vd2mdu	1634	33	/	/	SYM
cord-014597-66vd2mdu	1634	34	copy	copy	VBP
cord-014597-66vd2mdu	1634	35	in	in	IN
cord-014597-66vd2mdu	1634	36	a	a	DT
cord-014597-66vd2mdu	1634	37	batch	batch	NN
cord-014597-66vd2mdu	1634	38	culture	culture	NN
cord-014597-66vd2mdu	1634	39	,	,	,
cord-014597-66vd2mdu	1634	40	respectively	respectively	RB
cord-014597-66vd2mdu	1634	41	.	.	.
cord-014597-66vd2mdu	1635	1	The	the	DT
cord-014597-66vd2mdu	1635	2	productivity	productivity	NN
cord-014597-66vd2mdu	1635	3	per	per	IN
cord-014597-66vd2mdu	1635	4	integrated	integrate	VBN
cord-014597-66vd2mdu	1635	5	target	target	NN
cord-014597-66vd2mdu	1635	6	gene	gene	NN
cord-014597-66vd2mdu	1635	7	in	in	IN
cord-014597-66vd2mdu	1635	8	cell	cell	NN
cord-014597-66vd2mdu	1635	9	clones	clone	NNS
cord-014597-66vd2mdu	1635	10	developed	develop	VBN
cord-014597-66vd2mdu	1635	11	by	by	IN
cord-014597-66vd2mdu	1635	12	the	the	DT
cord-014597-66vd2mdu	1635	13	transposon	transposon	NN
cord-014597-66vd2mdu	1635	14	system	system	NN
cord-014597-66vd2mdu	1635	15	was	be	VBD
cord-014597-66vd2mdu	1635	16	much	much	RB
cord-014597-66vd2mdu	1635	17	higher	high	JJR
cord-014597-66vd2mdu	1635	18	than	than	IN
cord-014597-66vd2mdu	1635	19	that	that	DT
cord-014597-66vd2mdu	1635	20	in	in	IN
cord-014597-66vd2mdu	1635	21	cell	cell	NN
cord-014597-66vd2mdu	1635	22	clones	clone	NNS
cord-014597-66vd2mdu	1635	23	developed	develop	VBN
cord-014597-66vd2mdu	1635	24	by	by	IN
cord-014597-66vd2mdu	1635	25	random	random	JJ
cord-014597-66vd2mdu	1635	26	integration	integration	NN
cord-014597-66vd2mdu	1635	27	(	(	-LRB-
cord-014597-66vd2mdu	1635	28	Fig	Fig	NNP
cord-014597-66vd2mdu	1635	29	.	.	.
cord-014597-66vd2mdu	1635	30	1a	1a	CD
cord-014597-66vd2mdu	1635	31	and	and	CC
cord-014597-66vd2mdu	1635	32	b	b	LS
cord-014597-66vd2mdu	1635	33	)	)	-RRB-
cord-014597-66vd2mdu	1635	34	.	.	.
cord-014597-66vd2mdu	1636	1	To	to	TO
cord-014597-66vd2mdu	1636	2	evaluate	evaluate	VB
cord-014597-66vd2mdu	1636	3	the	the	DT
cord-014597-66vd2mdu	1636	4	productivity	productivity	NN
cord-014597-66vd2mdu	1636	5	stability	stability	NN
cord-014597-66vd2mdu	1636	6	of	of	IN
cord-014597-66vd2mdu	1636	7	cell	cell	NN
cord-014597-66vd2mdu	1636	8	clones	clone	NNS
cord-014597-66vd2mdu	1636	9	developed	develop	VBN
cord-014597-66vd2mdu	1636	10	by	by	IN
cord-014597-66vd2mdu	1636	11	the	the	DT
cord-014597-66vd2mdu	1636	12	transposon	transposon	NN
cord-014597-66vd2mdu	1636	13	system	system	NN
cord-014597-66vd2mdu	1636	14	,	,	,
cord-014597-66vd2mdu	1636	15	ten	ten	CD
cord-014597-66vd2mdu	1636	16	cell	cell	NN
cord-014597-66vd2mdu	1636	17	clones	clone	NNS
cord-014597-66vd2mdu	1636	18	at	at	IN
cord-014597-66vd2mdu	1636	19	generation	generation	NN
cord-014597-66vd2mdu	1636	20	0	0	CD
cord-014597-66vd2mdu	1636	21	,	,	,
cord-014597-66vd2mdu	1636	22	30	30	CD
cord-014597-66vd2mdu	1636	23	,	,	,
cord-014597-66vd2mdu	1636	24	60	60	CD
cord-014597-66vd2mdu	1636	25	,	,	,
cord-014597-66vd2mdu	1636	26	and	and	CC
cord-014597-66vd2mdu	1636	27	100	100	CD
cord-014597-66vd2mdu	1636	28	were	be	VBD
cord-014597-66vd2mdu	1636	29	applied	apply	VBN
cord-014597-66vd2mdu	1636	30	in	in	IN
cord-014597-66vd2mdu	1636	31	the	the	DT
cord-014597-66vd2mdu	1636	32	analysis	analysis	NN
cord-014597-66vd2mdu	1636	33	.	.	.
cord-014597-66vd2mdu	1637	1	Of	of	IN
cord-014597-66vd2mdu	1637	2	interest	interest	NN
cord-014597-66vd2mdu	1637	3	,	,	,
cord-014597-66vd2mdu	1637	4	about	about	RB
cord-014597-66vd2mdu	1637	5	80	80	CD
cord-014597-66vd2mdu	1637	6	%	%	NN
cord-014597-66vd2mdu	1637	7	of	of	IN
cord-014597-66vd2mdu	1637	8	cell	cell	NN
cord-014597-66vd2mdu	1637	9	clones	clone	NNS
cord-014597-66vd2mdu	1637	10	were	be	VBD
cord-014597-66vd2mdu	1637	11	stable	stable	JJ
cord-014597-66vd2mdu	1637	12	at	at	IN
cord-014597-66vd2mdu	1637	13	generation	generation	NN
cord-014597-66vd2mdu	1637	14	60	60	CD
cord-014597-66vd2mdu	1637	15	,	,	,
cord-014597-66vd2mdu	1637	16	but	but	CC
cord-014597-66vd2mdu	1637	17	lost	lose	VBD
cord-014597-66vd2mdu	1637	18	the	the	DT
cord-014597-66vd2mdu	1637	19	productivity	productivity	NN
cord-014597-66vd2mdu	1637	20	at	at	IN
cord-014597-66vd2mdu	1637	21	generation	generation	NN
cord-014597-66vd2mdu	1637	22	100	100	CD
cord-014597-66vd2mdu	1638	1	(	(	-LRB-
cord-014597-66vd2mdu	1638	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1638	3	.	.	.
cord-014597-66vd2mdu	1638	4	1c	1c	NNP
cord-014597-66vd2mdu	1638	5	)	)	-RRB-
cord-014597-66vd2mdu	1638	6	,	,	,
cord-014597-66vd2mdu	1638	7	implying	imply	VBG
cord-014597-66vd2mdu	1638	8	the	the	DT
cord-014597-66vd2mdu	1638	9	most	most	JJS
cord-014597-66vd2mdu	1638	10	cell	cell	NN
cord-014597-66vd2mdu	1638	11	clones	clone	NNS
cord-014597-66vd2mdu	1638	12	could	could	MD
cord-014597-66vd2mdu	1638	13	maintain	maintain	VB
cord-014597-66vd2mdu	1638	14	the	the	DT
cord-014597-66vd2mdu	1638	15	stability	stability	NN
cord-014597-66vd2mdu	1638	16	within	within	IN
cord-014597-66vd2mdu	1638	17	2	2	CD
cord-014597-66vd2mdu	1638	18	months	month	NNS
cord-014597-66vd2mdu	1638	19	.	.	.
cord-014597-66vd2mdu	1639	1	Using	use	VBG
cord-014597-66vd2mdu	1639	2	the	the	DT
cord-014597-66vd2mdu	1639	3	optimized	optimize	VBN
cord-014597-66vd2mdu	1639	4	conditions	condition	NNS
cord-014597-66vd2mdu	1639	5	of	of	IN
cord-014597-66vd2mdu	1639	6	the	the	DT
cord-014597-66vd2mdu	1639	7	transposon	transposon	NN
cord-014597-66vd2mdu	1639	8	system	system	NN
cord-014597-66vd2mdu	1639	9	to	to	TO
cord-014597-66vd2mdu	1639	10	develop	develop	VB
cord-014597-66vd2mdu	1639	11	the	the	DT
cord-014597-66vd2mdu	1639	12	stable	stable	JJ
cord-014597-66vd2mdu	1639	13	gene	gene	NN
cord-014597-66vd2mdu	1639	14	expression	expression	NN
cord-014597-66vd2mdu	1639	15	cells	cell	NNS
cord-014597-66vd2mdu	1639	16	,	,	,
cord-014597-66vd2mdu	1639	17	the	the	DT
cord-014597-66vd2mdu	1639	18	productivity	productivity	NN
cord-014597-66vd2mdu	1639	19	per	per	IN
cord-014597-66vd2mdu	1639	20	integrated	integrate	VBN
cord-014597-66vd2mdu	1639	21	target	target	NN
cord-014597-66vd2mdu	1639	22	gene	gene	NN
cord-014597-66vd2mdu	1639	23	was	be	VBD
cord-014597-66vd2mdu	1639	24	higher	high	JJR
cord-014597-66vd2mdu	1639	25	than	than	IN
cord-014597-66vd2mdu	1639	26	random	random	JJ
cord-014597-66vd2mdu	1639	27	integration	integration	NN
cord-014597-66vd2mdu	1639	28	.	.	.
cord-014597-66vd2mdu	1640	1	These	these	DT
cord-014597-66vd2mdu	1640	2	results	result	NNS
cord-014597-66vd2mdu	1640	3	suggested	suggest	VBD
cord-014597-66vd2mdu	1640	4	that	that	IN
cord-014597-66vd2mdu	1640	5	our	-PRON-	PRP$
cord-014597-66vd2mdu	1640	6	platform	platform	NN
cord-014597-66vd2mdu	1640	7	is	be	VBZ
cord-014597-66vd2mdu	1640	8	capable	capable	JJ
cord-014597-66vd2mdu	1640	9	to	to	TO
cord-014597-66vd2mdu	1640	10	develop	develop	VB
cord-014597-66vd2mdu	1640	11	high	high	JJ
cord-014597-66vd2mdu	1640	12	yield	yield	NN
cord-014597-66vd2mdu	1640	13	producing	produce	VBG
cord-014597-66vd2mdu	1640	14	cells	cell	NNS
cord-014597-66vd2mdu	1640	15	with	with	IN
cord-014597-66vd2mdu	1640	16	1	1	CD
cord-014597-66vd2mdu	1640	17	-	-	SYM
cord-014597-66vd2mdu	1640	18	2	2	CD
cord-014597-66vd2mdu	1640	19	copy	copy	NN
cord-014597-66vd2mdu	1640	20	of	of	IN
cord-014597-66vd2mdu	1640	21	integrated	integrated	JJ
cord-014597-66vd2mdu	1640	22	target	target	NN
cord-014597-66vd2mdu	1640	23	antibody	antibody	NN
cord-014597-66vd2mdu	1640	24	gene	gene	NN
cord-014597-66vd2mdu	1640	25	and	and	CC
cord-014597-66vd2mdu	1640	26	can	can	MD
cord-014597-66vd2mdu	1640	27	be	be	VB
cord-014597-66vd2mdu	1640	28	applied	apply	VBN
cord-014597-66vd2mdu	1640	29	to	to	TO
cord-014597-66vd2mdu	1640	30	identify	identify	VB
cord-014597-66vd2mdu	1640	31	high	high	JJ
cord-014597-66vd2mdu	1640	32	yield	yield	NN
cord-014597-66vd2mdu	1640	33	integration	integration	NN
cord-014597-66vd2mdu	1640	34	sites	site	NNS
cord-014597-66vd2mdu	1640	35	.	.	.
cord-014597-66vd2mdu	1641	1	Background	Background	NNP
cord-014597-66vd2mdu	1641	2	Mammalian	mammalian	JJ
cord-014597-66vd2mdu	1641	3	cells	cell	NNS
cord-014597-66vd2mdu	1641	4	show	show	VBP
cord-014597-66vd2mdu	1641	5	an	an	DT
cord-014597-66vd2mdu	1641	6	inefficient	inefficient	JJ
cord-014597-66vd2mdu	1641	7	metabolism	metabolism	NN
cord-014597-66vd2mdu	1641	8	characterized	characterize	VBN
cord-014597-66vd2mdu	1641	9	by	by	IN
cord-014597-66vd2mdu	1641	10	high	high	JJ
cord-014597-66vd2mdu	1641	11	glucose	glucose	NN
cord-014597-66vd2mdu	1641	12	uptake	uptake	NN
cord-014597-66vd2mdu	1641	13	and	and	CC
cord-014597-66vd2mdu	1641	14	the	the	DT
cord-014597-66vd2mdu	1641	15	production	production	NN
cord-014597-66vd2mdu	1641	16	of	of	IN
cord-014597-66vd2mdu	1641	17	high	high	JJ
cord-014597-66vd2mdu	1641	18	amounts	amount	NNS
cord-014597-66vd2mdu	1641	19	of	of	IN
cord-014597-66vd2mdu	1641	20	lactate	lactate	NN
cord-014597-66vd2mdu	1641	21	,	,	,
cord-014597-66vd2mdu	1641	22	a	a	DT
cord-014597-66vd2mdu	1641	23	widely	widely	RB
cord-014597-66vd2mdu	1641	24	known	know	VBN
cord-014597-66vd2mdu	1641	25	growth	growth	NN
cord-014597-66vd2mdu	1641	26	inhibition	inhibition	NN
cord-014597-66vd2mdu	1641	27	by	by	IN
cord-014597-66vd2mdu	1641	28	-	-	HYPH
cord-014597-66vd2mdu	1641	29	product	product	NN
cord-014597-66vd2mdu	1641	30	[	[	-LRB-
cord-014597-66vd2mdu	1641	31	1	1	CD
cord-014597-66vd2mdu	1641	32	]	]	-RRB-
cord-014597-66vd2mdu	1641	33	.	.	.
cord-014597-66vd2mdu	1642	1	Recently	recently	RB
cord-014597-66vd2mdu	1642	2	,	,	,
cord-014597-66vd2mdu	1642	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1642	4	have	have	VBP
cord-014597-66vd2mdu	1642	5	observed	observe	VBN
cord-014597-66vd2mdu	1642	6	a	a	DT
cord-014597-66vd2mdu	1642	7	different	different	JJ
cord-014597-66vd2mdu	1642	8	glucose	glucose	NN
cord-014597-66vd2mdu	1642	9	-	-	HYPH
cord-014597-66vd2mdu	1642	10	lactate	lactate	NN
cord-014597-66vd2mdu	1642	11	metabolism	metabolism	NN
cord-014597-66vd2mdu	1642	12	in	in	IN
cord-014597-66vd2mdu	1642	13	some	some	DT
cord-014597-66vd2mdu	1642	14	cell	cell	NN
cord-014597-66vd2mdu	1642	15	lines	line	NNS
cord-014597-66vd2mdu	1642	16	.	.	.
cord-014597-66vd2mdu	1643	1	While	while	IN
cord-014597-66vd2mdu	1643	2	some	some	DT
cord-014597-66vd2mdu	1643	3	cell	cell	NN
cord-014597-66vd2mdu	1643	4	lines	line	NNS
cord-014597-66vd2mdu	1643	5	are	be	VBP
cord-014597-66vd2mdu	1643	6	unable	unable	JJ
cord-014597-66vd2mdu	1643	7	to	to	TO
cord-014597-66vd2mdu	1643	8	metabolize	metabolize	VB
cord-014597-66vd2mdu	1643	9	lactate	lactate	NN
cord-014597-66vd2mdu	1643	10	,	,	,
cord-014597-66vd2mdu	1643	11	others	other	NNS
cord-014597-66vd2mdu	1643	12	can	can	MD
cord-014597-66vd2mdu	1643	13	co	co	VB
cord-014597-66vd2mdu	1643	14	-	-	VB
cord-014597-66vd2mdu	1643	15	metabolize	metabolize	VB
cord-014597-66vd2mdu	1643	16	simultaneously	simultaneously	RB
cord-014597-66vd2mdu	1643	17	glucose	glucose	NN
cord-014597-66vd2mdu	1643	18	and	and	CC
cord-014597-66vd2mdu	1643	19	lactate	lactate	NN
cord-014597-66vd2mdu	1643	20	under	under	IN
cord-014597-66vd2mdu	1643	21	certain	certain	JJ
cord-014597-66vd2mdu	1643	22	culture	culture	NN
cord-014597-66vd2mdu	1643	23	conditions	condition	NNS
cord-014597-66vd2mdu	1643	24	,	,	,
cord-014597-66vd2mdu	1643	25	even	even	RB
cord-014597-66vd2mdu	1643	26	during	during	IN
cord-014597-66vd2mdu	1643	27	the	the	DT
cord-014597-66vd2mdu	1643	28	exponential	exponential	JJ
cord-014597-66vd2mdu	1643	29	growth	growth	NN
cord-014597-66vd2mdu	1643	30	phase	phase	NN
cord-014597-66vd2mdu	1643	31	[	[	-LRB-
cord-014597-66vd2mdu	1643	32	2	2	CD
cord-014597-66vd2mdu	1643	33	]	]	-RRB-
cord-014597-66vd2mdu	1643	34	.	.	.
cord-014597-66vd2mdu	1644	1	These	these	DT
cord-014597-66vd2mdu	1644	2	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1644	3	differences	difference	NNS
cord-014597-66vd2mdu	1644	4	between	between	IN
cord-014597-66vd2mdu	1644	5	different	different	JJ
cord-014597-66vd2mdu	1644	6	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1644	7	cell	cell	NN
cord-014597-66vd2mdu	1644	8	lines	line	NNS
cord-014597-66vd2mdu	1644	9	(	(	-LRB-
cord-014597-66vd2mdu	1644	10	CHO	CHO	NNP
cord-014597-66vd2mdu	1644	11	,	,	,
cord-014597-66vd2mdu	1644	12	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1644	13	and	and	CC
cord-014597-66vd2mdu	1644	14	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	1644	15	)	)	-RRB-
cord-014597-66vd2mdu	1644	16	have	have	VBP
cord-014597-66vd2mdu	1644	17	been	be	VBN
cord-014597-66vd2mdu	1644	18	studied	study	VBN
cord-014597-66vd2mdu	1644	19	by	by	IN
cord-014597-66vd2mdu	1644	20	means	mean	NNS
cord-014597-66vd2mdu	1644	21	of	of	IN
cord-014597-66vd2mdu	1644	22	Flux	Flux	NNP
cord-014597-66vd2mdu	1644	23	Balance	Balance	NNP
cord-014597-66vd2mdu	1644	24	Analysis	Analysis	NNP
cord-014597-66vd2mdu	1644	25	(	(	-LRB-
cord-014597-66vd2mdu	1644	26	FBA	FBA	NNP
cord-014597-66vd2mdu	1644	27	)	)	-RRB-
cord-014597-66vd2mdu	1644	28	.	.	.
cord-014597-66vd2mdu	1645	1	Three	three	CD
cord-014597-66vd2mdu	1645	2	different	different	JJ
cord-014597-66vd2mdu	1645	3	cell	cell	NN
cord-014597-66vd2mdu	1645	4	lines	line	NNS
cord-014597-66vd2mdu	1645	5	were	be	VBD
cord-014597-66vd2mdu	1645	6	cultured	culture	VBN
cord-014597-66vd2mdu	1645	7	in	in	IN
cord-014597-66vd2mdu	1645	8	a	a	DT
cord-014597-66vd2mdu	1645	9	2-liter	2-liter	CD
cord-014597-66vd2mdu	1645	10	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	1645	11	:	:	:
cord-014597-66vd2mdu	1645	12	CHO	CHO	NNP
cord-014597-66vd2mdu	1645	13	-	-	HYPH
cord-014597-66vd2mdu	1645	14	S	S	NNP
cord-014597-66vd2mdu	1645	15	,	,	,
cord-014597-66vd2mdu	1645	16	HEK293SF	HEK293SF	NNP
cord-014597-66vd2mdu	1645	17	and	and	CC
cord-014597-66vd2mdu	1645	18	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	1645	19	KB-26.5	KB-26.5	NNS
cord-014597-66vd2mdu	1645	20	.	.	.
cord-014597-66vd2mdu	1646	1	For	for	IN
cord-014597-66vd2mdu	1646	2	the	the	DT
cord-014597-66vd2mdu	1646	3	FBA	FBA	NNP
cord-014597-66vd2mdu	1646	4	,	,	,
cord-014597-66vd2mdu	1646	5	two	two	CD
cord-014597-66vd2mdu	1646	6	adapted	adapt	VBN
cord-014597-66vd2mdu	1646	7	genome	genome	NN
cord-014597-66vd2mdu	1646	8	-	-	HYPH
cord-014597-66vd2mdu	1646	9	scale	scale	NN
cord-014597-66vd2mdu	1646	10	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1646	11	models	model	NNS
cord-014597-66vd2mdu	1646	12	were	be	VBD
cord-014597-66vd2mdu	1646	13	used	use	VBN
cord-014597-66vd2mdu	1646	14	:	:	:
cord-014597-66vd2mdu	1646	15	a	a	DT
cord-014597-66vd2mdu	1646	16	reconstruction	reconstruction	NN
cord-014597-66vd2mdu	1646	17	of	of	IN
cord-014597-66vd2mdu	1646	18	Mus	Mus	NNP
cord-014597-66vd2mdu	1646	19	musculus	musculus	NN
cord-014597-66vd2mdu	1646	20	for	for	IN
cord-014597-66vd2mdu	1646	21	CHO	CHO	NNP
cord-014597-66vd2mdu	1646	22	and	and	CC
cord-014597-66vd2mdu	1646	23	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	1646	24	[	[	-LRB-
cord-014597-66vd2mdu	1646	25	3	3	CD
cord-014597-66vd2mdu	1646	26	]	]	-RRB-
cord-014597-66vd2mdu	1646	27	,	,	,
cord-014597-66vd2mdu	1646	28	and	and	CC
cord-014597-66vd2mdu	1646	29	a	a	DT
cord-014597-66vd2mdu	1646	30	reconstruction	reconstruction	NN
cord-014597-66vd2mdu	1646	31	of	of	IN
cord-014597-66vd2mdu	1646	32	Human	human	JJ
cord-014597-66vd2mdu	1646	33	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1646	34	model	model	NN
cord-014597-66vd2mdu	1646	35	(	(	-LRB-
cord-014597-66vd2mdu	1646	36	Recon	Recon	NNP
cord-014597-66vd2mdu	1646	37	2	2	CD
cord-014597-66vd2mdu	1646	38	)	)	-RRB-
cord-014597-66vd2mdu	1646	39	for	for	IN
cord-014597-66vd2mdu	1646	40	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1646	41	[	[	-LRB-
cord-014597-66vd2mdu	1646	42	4	4	CD
cord-014597-66vd2mdu	1646	43	]	]	-RRB-
cord-014597-66vd2mdu	1646	44	.	.	.
cord-014597-66vd2mdu	1647	1	In	in	IN
cord-014597-66vd2mdu	1647	2	cultures	culture	NNS
cord-014597-66vd2mdu	1647	3	where	where	WRB
cord-014597-66vd2mdu	1647	4	pH	pH	NNP
cord-014597-66vd2mdu	1647	5	was	be	VBD
cord-014597-66vd2mdu	1647	6	not	not	RB
cord-014597-66vd2mdu	1647	7	controlled	control	VBN
cord-014597-66vd2mdu	1647	8	,	,	,
cord-014597-66vd2mdu	1647	9	two	two	CD
cord-014597-66vd2mdu	1647	10	different	different	JJ
cord-014597-66vd2mdu	1647	11	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1647	12	phases	phase	NNS
cord-014597-66vd2mdu	1647	13	were	be	VBD
cord-014597-66vd2mdu	1647	14	observed	observe	VBN
cord-014597-66vd2mdu	1647	15	for	for	IN
cord-014597-66vd2mdu	1647	16	CHO	CHO	NNP
cord-014597-66vd2mdu	1647	17	and	and	CC
cord-014597-66vd2mdu	1647	18	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1647	19	cells	cell	NNS
cord-014597-66vd2mdu	1647	20	.	.	.
cord-014597-66vd2mdu	1648	1	During	during	IN
cord-014597-66vd2mdu	1648	2	the	the	DT
cord-014597-66vd2mdu	1648	3	first	first	JJ
cord-014597-66vd2mdu	1648	4	phase	phase	NN
cord-014597-66vd2mdu	1648	5	both	both	DT
cord-014597-66vd2mdu	1648	6	cell	cell	NN
cord-014597-66vd2mdu	1648	7	lines	line	NNS
cord-014597-66vd2mdu	1648	8	produced	produce	VBD
cord-014597-66vd2mdu	1648	9	large	large	JJ
cord-014597-66vd2mdu	1648	10	amounts	amount	NNS
cord-014597-66vd2mdu	1648	11	of	of	IN
cord-014597-66vd2mdu	1648	12	lactate	lactate	NN
cord-014597-66vd2mdu	1648	13	as	as	IN
cord-014597-66vd2mdu	1648	14	a	a	DT
cord-014597-66vd2mdu	1648	15	consequence	consequence	NN
cord-014597-66vd2mdu	1648	16	of	of	IN
cord-014597-66vd2mdu	1648	17	the	the	DT
cord-014597-66vd2mdu	1648	18	high	high	JJ
cord-014597-66vd2mdu	1648	19	glucose	glucose	NN
cord-014597-66vd2mdu	1648	20	consumption	consumption	NN
cord-014597-66vd2mdu	1648	21	rates	rate	NNS
cord-014597-66vd2mdu	1648	22	.	.	.
cord-014597-66vd2mdu	1649	1	Interestingly	interestingly	RB
cord-014597-66vd2mdu	1649	2	,	,	,
cord-014597-66vd2mdu	1649	3	when	when	WRB
cord-014597-66vd2mdu	1649	4	pH	pH	NNP
cord-014597-66vd2mdu	1649	5	dropped	drop	VBD
cord-014597-66vd2mdu	1649	6	below	below	IN
cord-014597-66vd2mdu	1649	7	6.8	6.8	CD
cord-014597-66vd2mdu	1649	8	,	,	,
cord-014597-66vd2mdu	1649	9	due	due	JJ
cord-014597-66vd2mdu	1649	10	to	to	IN
cord-014597-66vd2mdu	1649	11	acid	acid	JJ
cord-014597-66vd2mdu	1649	12	lactic	lactic	JJ
cord-014597-66vd2mdu	1649	13	secretion	secretion	NN
cord-014597-66vd2mdu	1649	14	and	and	CC
cord-014597-66vd2mdu	1649	15	accumulation	accumulation	NN
cord-014597-66vd2mdu	1649	16	,	,	,
cord-014597-66vd2mdu	1649	17	a	a	DT
cord-014597-66vd2mdu	1649	18	second	second	JJ
cord-014597-66vd2mdu	1649	19	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1649	20	phase	phase	NN
cord-014597-66vd2mdu	1649	21	was	be	VBD
cord-014597-66vd2mdu	1649	22	identified	identify	VBN
cord-014597-66vd2mdu	1649	23	,	,	,
cord-014597-66vd2mdu	1649	24	in	in	IN
cord-014597-66vd2mdu	1649	25	which	which	WDT
cord-014597-66vd2mdu	1649	26	concomitant	concomitant	JJ
cord-014597-66vd2mdu	1649	27	consumption	consumption	NN
cord-014597-66vd2mdu	1649	28	of	of	IN
cord-014597-66vd2mdu	1649	29	glucose	glucose	NN
cord-014597-66vd2mdu	1649	30	and	and	CC
cord-014597-66vd2mdu	1649	31	lactate	lactate	NN
cord-014597-66vd2mdu	1649	32	was	be	VBD
cord-014597-66vd2mdu	1649	33	observed	observe	VBN
cord-014597-66vd2mdu	1649	34	even	even	RB
cord-014597-66vd2mdu	1649	35	during	during	IN
cord-014597-66vd2mdu	1649	36	the	the	DT
cord-014597-66vd2mdu	1649	37	exponential	exponential	JJ
cord-014597-66vd2mdu	1649	38	growth	growth	NN
cord-014597-66vd2mdu	1649	39	phase	phase	NN
cord-014597-66vd2mdu	1649	40	.	.	.
cord-014597-66vd2mdu	1650	1	Conversely	conversely	RB
cord-014597-66vd2mdu	1650	2	,	,	,
cord-014597-66vd2mdu	1650	3	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	1650	4	cells	cell	NNS
cord-014597-66vd2mdu	1650	5	were	be	VBD
cord-014597-66vd2mdu	1650	6	unable	unable	JJ
cord-014597-66vd2mdu	1650	7	to	to	TO
cord-014597-66vd2mdu	1650	8	co	co	VB
cord-014597-66vd2mdu	1650	9	-	-	VB
cord-014597-66vd2mdu	1650	10	consume	consume	VB
cord-014597-66vd2mdu	1650	11	lactate	lactate	NN
cord-014597-66vd2mdu	1650	12	and	and	CC
cord-014597-66vd2mdu	1650	13	glucose	glucose	NN
cord-014597-66vd2mdu	1650	14	simultaneously	simultaneously	RB
cord-014597-66vd2mdu	1650	15	even	even	RB
cord-014597-66vd2mdu	1650	16	under	under	IN
cord-014597-66vd2mdu	1650	17	noncontrolled	noncontrolled	JJ
cord-014597-66vd2mdu	1650	18	pH	pH	NNP
cord-014597-66vd2mdu	1650	19	conditions	condition	NNS
cord-014597-66vd2mdu	1650	20	.	.	.
cord-014597-66vd2mdu	1651	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1651	2	,	,	,
cord-014597-66vd2mdu	1651	3	the	the	DT
cord-014597-66vd2mdu	1651	4	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	1651	5	physiological	physiological	JJ
cord-014597-66vd2mdu	1651	6	data	datum	NNS
cord-014597-66vd2mdu	1651	7	used	use	VBN
cord-014597-66vd2mdu	1651	8	for	for	IN
cord-014597-66vd2mdu	1651	9	the	the	DT
cord-014597-66vd2mdu	1651	10	FBA	FBA	NNP
cord-014597-66vd2mdu	1651	11	corresponded	correspond	VBD
cord-014597-66vd2mdu	1651	12	to	to	IN
cord-014597-66vd2mdu	1651	13	only	only	RB
cord-014597-66vd2mdu	1651	14	phase	phase	NN
cord-014597-66vd2mdu	1651	15	1	1	CD
cord-014597-66vd2mdu	1651	16	of	of	IN
cord-014597-66vd2mdu	1651	17	pHcontrolled	phcontrolle	VBN
cord-014597-66vd2mdu	1651	18	cultures	culture	NNS
cord-014597-66vd2mdu	1651	19	.	.	.
cord-014597-66vd2mdu	1652	1	A	a	DT
cord-014597-66vd2mdu	1652	2	summary	summary	NN
cord-014597-66vd2mdu	1652	3	of	of	IN
cord-014597-66vd2mdu	1652	4	the	the	DT
cord-014597-66vd2mdu	1652	5	main	main	JJ
cord-014597-66vd2mdu	1652	6	cell	cell	NN
cord-014597-66vd2mdu	1652	7	growth	growth	NN
cord-014597-66vd2mdu	1652	8	and	and	CC
cord-014597-66vd2mdu	1652	9	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1652	10	parameters	parameter	NNS
cord-014597-66vd2mdu	1652	11	obtained	obtain	VBN
cord-014597-66vd2mdu	1652	12	from	from	IN
cord-014597-66vd2mdu	1652	13	the	the	DT
cord-014597-66vd2mdu	1652	14	different	different	JJ
cord-014597-66vd2mdu	1652	15	experiments	experiment	NNS
cord-014597-66vd2mdu	1652	16	performed	perform	VBN
cord-014597-66vd2mdu	1652	17	is	be	VBZ
cord-014597-66vd2mdu	1652	18	presented	present	VBN
cord-014597-66vd2mdu	1652	19	in	in	IN
cord-014597-66vd2mdu	1652	20	Table	Table	NNP
cord-014597-66vd2mdu	1652	21	1	1	CD
cord-014597-66vd2mdu	1652	22	.	.	.
cord-014597-66vd2mdu	1653	1	FBA	FBA	NNP
cord-014597-66vd2mdu	1653	2	shows	show	VBZ
cord-014597-66vd2mdu	1653	3	(	(	-LRB-
cord-014597-66vd2mdu	1653	4	Fig	Fig	NNP
cord-014597-66vd2mdu	1653	5	.	.	.
cord-014597-66vd2mdu	1653	6	1	1	CD
cord-014597-66vd2mdu	1653	7	for	for	IN
cord-014597-66vd2mdu	1653	8	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1653	9	cell	cell	NN
cord-014597-66vd2mdu	1653	10	culture	culture	NN
cord-014597-66vd2mdu	1653	11	)	)	-RRB-
cord-014597-66vd2mdu	1653	12	that	that	IN
cord-014597-66vd2mdu	1653	13	lactate	lactate	NN
cord-014597-66vd2mdu	1653	14	is	be	VBZ
cord-014597-66vd2mdu	1653	15	produced	produce	VBN
cord-014597-66vd2mdu	1653	16	in	in	IN
cord-014597-66vd2mdu	1653	17	phase	phase	NN
cord-014597-66vd2mdu	1653	18	1	1	CD
cord-014597-66vd2mdu	1653	19	because	because	IN
cord-014597-66vd2mdu	1653	20	pyruvate	pyruvate	NNP
cord-014597-66vd2mdu	1653	21	has	have	VBZ
cord-014597-66vd2mdu	1653	22	to	to	TO
cord-014597-66vd2mdu	1653	23	be	be	VB
cord-014597-66vd2mdu	1653	24	converted	convert	VBN
cord-014597-66vd2mdu	1653	25	to	to	IN
cord-014597-66vd2mdu	1653	26	lactate	lactate	NN
cord-014597-66vd2mdu	1653	27	to	to	TO
cord-014597-66vd2mdu	1653	28	fulfill	fulfill	VB
cord-014597-66vd2mdu	1653	29	the	the	DT
cord-014597-66vd2mdu	1653	30	NADH	NADH	NNP
cord-014597-66vd2mdu	1653	31	regeneration	regeneration	NN
cord-014597-66vd2mdu	1653	32	in	in	IN
cord-014597-66vd2mdu	1653	33	the	the	DT
cord-014597-66vd2mdu	1653	34	cytoplasm	cytoplasm	NN
cord-014597-66vd2mdu	1653	35	and	and	CC
cord-014597-66vd2mdu	1653	36	only	only	RB
cord-014597-66vd2mdu	1653	37	a	a	DT
cord-014597-66vd2mdu	1653	38	small	small	JJ
cord-014597-66vd2mdu	1653	39	amount	amount	NN
cord-014597-66vd2mdu	1653	40	of	of	IN
cord-014597-66vd2mdu	1653	41	pyruvate	pyruvate	NN
cord-014597-66vd2mdu	1653	42	can	can	MD
cord-014597-66vd2mdu	1653	43	be	be	VB
cord-014597-66vd2mdu	1653	44	transported	transport	VBN
cord-014597-66vd2mdu	1653	45	into	into	IN
cord-014597-66vd2mdu	1653	46	TCA	TCA	NNP
cord-014597-66vd2mdu	1653	47	through	through	IN
cord-014597-66vd2mdu	1653	48	Acetyl	Acetyl	NNP
cord-014597-66vd2mdu	1653	49	-	-	HYPH
cord-014597-66vd2mdu	1653	50	CoA.	CoA.	NNP
cord-014597-66vd2mdu	1653	51	Cell	Cell	NNP
cord-014597-66vd2mdu	1653	52	metabolism	metabolism	NN
cord-014597-66vd2mdu	1653	53	in	in	IN
cord-014597-66vd2mdu	1653	54	phase	phase	NN
cord-014597-66vd2mdu	1653	55	1	1	CD
cord-014597-66vd2mdu	1653	56	is	be	VBZ
cord-014597-66vd2mdu	1653	57	highly	highly	RB
cord-014597-66vd2mdu	1653	58	inefficient	inefficient	JJ
cord-014597-66vd2mdu	1653	59	,	,	,
cord-014597-66vd2mdu	1653	60	as	as	IN
cord-014597-66vd2mdu	1653	61	the	the	DT
cord-014597-66vd2mdu	1653	62	majority	majority	NN
cord-014597-66vd2mdu	1653	63	of	of	IN
cord-014597-66vd2mdu	1653	64	the	the	DT
cord-014597-66vd2mdu	1653	65	carbon	carbon	NN
cord-014597-66vd2mdu	1653	66	source	source	NN
cord-014597-66vd2mdu	1653	67	is	be	VBZ
cord-014597-66vd2mdu	1653	68	not	not	RB
cord-014597-66vd2mdu	1653	69	used	use	VBN
cord-014597-66vd2mdu	1653	70	for	for	IN
cord-014597-66vd2mdu	1653	71	the	the	DT
cord-014597-66vd2mdu	1653	72	generation	generation	NN
cord-014597-66vd2mdu	1653	73	of	of	IN
cord-014597-66vd2mdu	1653	74	energy	energy	NN
cord-014597-66vd2mdu	1653	75	nor	nor	CC
cord-014597-66vd2mdu	1653	76	biomass	biomass	NN
cord-014597-66vd2mdu	1653	77	.	.	.
cord-014597-66vd2mdu	1654	1	In	in	IN
cord-014597-66vd2mdu	1654	2	phase	phase	NN
cord-014597-66vd2mdu	1654	3	2	2	CD
cord-014597-66vd2mdu	1654	4	,	,	,
cord-014597-66vd2mdu	1654	5	in	in	IN
cord-014597-66vd2mdu	1654	6	which	which	WDT
cord-014597-66vd2mdu	1654	7	mitochondrial	mitochondrial	JJ
cord-014597-66vd2mdu	1654	8	LDH	LDH	NNP
cord-014597-66vd2mdu	1654	9	was	be	VBD
cord-014597-66vd2mdu	1654	10	considered	consider	VBN
cord-014597-66vd2mdu	1654	11	,	,	,
cord-014597-66vd2mdu	1654	12	TCA	TCA	NNP
cord-014597-66vd2mdu	1654	13	fluxes	flux	NNS
cord-014597-66vd2mdu	1654	14	could	could	MD
cord-014597-66vd2mdu	1654	15	be	be	VB
cord-014597-66vd2mdu	1654	16	maintained	maintain	VBN
cord-014597-66vd2mdu	1654	17	as	as	IN
cord-014597-66vd2mdu	1654	18	in	in	IN
cord-014597-66vd2mdu	1654	19	phase	phase	NN
cord-014597-66vd2mdu	1654	20	1	1	CD
cord-014597-66vd2mdu	1654	21	at	at	IN
cord-014597-66vd2mdu	1654	22	the	the	DT
cord-014597-66vd2mdu	1654	23	maximal	maximal	JJ
cord-014597-66vd2mdu	1654	24	rate	rate	NN
cord-014597-66vd2mdu	1654	25	encountered	encounter	VBN
cord-014597-66vd2mdu	1654	26	;	;	:
cord-014597-66vd2mdu	1654	27	hence	hence	RB
cord-014597-66vd2mdu	1654	28	,	,	,
cord-014597-66vd2mdu	1654	29	the	the	DT
cord-014597-66vd2mdu	1654	30	energy	energy	NN
cord-014597-66vd2mdu	1654	31	available	available	JJ
cord-014597-66vd2mdu	1654	32	for	for	IN
cord-014597-66vd2mdu	1654	33	cells	cell	NNS
cord-014597-66vd2mdu	1654	34	to	to	TO
cord-014597-66vd2mdu	1654	35	grow	grow	VB
cord-014597-66vd2mdu	1654	36	was	be	VBD
cord-014597-66vd2mdu	1654	37	similar	similar	JJ
cord-014597-66vd2mdu	1654	38	in	in	IN
cord-014597-66vd2mdu	1654	39	both	both	DT
cord-014597-66vd2mdu	1654	40	phases	phase	NNS
cord-014597-66vd2mdu	1654	41	,	,	,
cord-014597-66vd2mdu	1654	42	obtaining	obtain	VBG
cord-014597-66vd2mdu	1654	43	similar	similar	JJ
cord-014597-66vd2mdu	1654	44	growth	growth	NN
cord-014597-66vd2mdu	1654	45	rate	rate	NN
cord-014597-66vd2mdu	1654	46	.	.	.
cord-014597-66vd2mdu	1655	1	Two	two	CD
cord-014597-66vd2mdu	1655	2	different	different	JJ
cord-014597-66vd2mdu	1655	3	glucose	glucose	NN
cord-014597-66vd2mdu	1655	4	and	and	CC
cord-014597-66vd2mdu	1655	5	lactate	lactate	NN
cord-014597-66vd2mdu	1655	6	metabolism	metabolism	NN
cord-014597-66vd2mdu	1655	7	behaviors	behavior	NNS
cord-014597-66vd2mdu	1655	8	have	have	VBP
cord-014597-66vd2mdu	1655	9	been	be	VBN
cord-014597-66vd2mdu	1655	10	observed	observe	VBN
cord-014597-66vd2mdu	1655	11	in	in	IN
cord-014597-66vd2mdu	1655	12	CHO	CHO	NNP
cord-014597-66vd2mdu	1655	13	and	and	CC
cord-014597-66vd2mdu	1655	14	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1655	15	cultures	culture	NNS
cord-014597-66vd2mdu	1655	16	depending	depend	VBG
cord-014597-66vd2mdu	1655	17	on	on	IN
cord-014597-66vd2mdu	1655	18	the	the	DT
cord-014597-66vd2mdu	1655	19	culture	culture	NN
cord-014597-66vd2mdu	1655	20	conditions	condition	NNS
cord-014597-66vd2mdu	1655	21	:	:	:
cord-014597-66vd2mdu	1655	22	phase	phase	NN
cord-014597-66vd2mdu	1655	23	1	1	CD
cord-014597-66vd2mdu	1655	24	)	)	-RRB-
cord-014597-66vd2mdu	1655	25	glucose	glucose	NN
cord-014597-66vd2mdu	1655	26	consumption	consumption	NN
cord-014597-66vd2mdu	1655	27	and	and	CC
cord-014597-66vd2mdu	1655	28	lactate	lactate	NN
cord-014597-66vd2mdu	1655	29	production	production	NN
cord-014597-66vd2mdu	1655	30	,	,	,
cord-014597-66vd2mdu	1655	31	and	and	CC
cord-014597-66vd2mdu	1655	32	phase	phase	NN
cord-014597-66vd2mdu	1655	33	2	2	CD
cord-014597-66vd2mdu	1655	34	)	)	-RRB-
cord-014597-66vd2mdu	1655	35	glucose	glucose	NN
cord-014597-66vd2mdu	1655	36	and	and	CC
cord-014597-66vd2mdu	1655	37	lactate	lactate	VB
cord-014597-66vd2mdu	1655	38	simultaneous	simultaneous	JJ
cord-014597-66vd2mdu	1655	39	consumption	consumption	NN
cord-014597-66vd2mdu	1655	40	.	.	.
cord-014597-66vd2mdu	1656	1	In	in	IN
cord-014597-66vd2mdu	1656	2	contrast	contrast	NN
cord-014597-66vd2mdu	1656	3	,	,	,
cord-014597-66vd2mdu	1656	4	only	only	RB
cord-014597-66vd2mdu	1656	5	phase	phase	NN
cord-014597-66vd2mdu	1656	6	1	1	CD
cord-014597-66vd2mdu	1656	7	was	be	VBD
cord-014597-66vd2mdu	1656	8	observed	observe	VBN
cord-014597-66vd2mdu	1656	9	in	in	IN
cord-014597-66vd2mdu	1656	10	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	1656	11	cultures	culture	NNS
cord-014597-66vd2mdu	1656	12	even	even	RB
cord-014597-66vd2mdu	1656	13	when	when	WRB
cord-014597-66vd2mdu	1656	14	pH	pH	NNP
cord-014597-66vd2mdu	1656	15	was	be	VBD
cord-014597-66vd2mdu	1656	16	non	non	JJ
cord-014597-66vd2mdu	1656	17	-	-	VBN
cord-014597-66vd2mdu	1656	18	controlled	control	VBN
cord-014597-66vd2mdu	1656	19	.	.	.
cord-014597-66vd2mdu	1657	1	FBA	FBA	NNP
cord-014597-66vd2mdu	1657	2	showed	show	VBD
cord-014597-66vd2mdu	1657	3	that	that	IN
cord-014597-66vd2mdu	1657	4	TCA	TCA	NNP
cord-014597-66vd2mdu	1657	5	fluxes	flux	NNS
cord-014597-66vd2mdu	1657	6	in	in	IN
cord-014597-66vd2mdu	1657	7	phase	phase	NN
cord-014597-66vd2mdu	1657	8	1	1	CD
cord-014597-66vd2mdu	1657	9	and	and	CC
cord-014597-66vd2mdu	1657	10	phase	phase	NN
cord-014597-66vd2mdu	1657	11	2	2	CD
cord-014597-66vd2mdu	1657	12	were	be	VBD
cord-014597-66vd2mdu	1657	13	similar	similar	JJ
cord-014597-66vd2mdu	1657	14	,	,	,
cord-014597-66vd2mdu	1657	15	obtaining	obtain	VBG
cord-014597-66vd2mdu	1657	16	similar	similar	JJ
cord-014597-66vd2mdu	1657	17	cell	cell	NN
cord-014597-66vd2mdu	1657	18	growth	growth	NN
cord-014597-66vd2mdu	1657	19	rate	rate	NN
cord-014597-66vd2mdu	1657	20	,	,	,
cord-014597-66vd2mdu	1657	21	but	but	CC
cord-014597-66vd2mdu	1657	22	glucose	glucose	NN
cord-014597-66vd2mdu	1657	23	uptake	uptake	NN
cord-014597-66vd2mdu	1657	24	rate	rate	NN
cord-014597-66vd2mdu	1657	25	was	be	VBD
cord-014597-66vd2mdu	1657	26	much	much	RB
cord-014597-66vd2mdu	1657	27	lower	low	JJR
cord-014597-66vd2mdu	1657	28	in	in	IN
cord-014597-66vd2mdu	1657	29	phase	phase	NN
cord-014597-66vd2mdu	1657	30	2	2	CD
cord-014597-66vd2mdu	1657	31	due	due	IN
cord-014597-66vd2mdu	1657	32	to	to	IN
cord-014597-66vd2mdu	1657	33	the	the	DT
cord-014597-66vd2mdu	1657	34	lactate	lactate	NN
cord-014597-66vd2mdu	1657	35	co	co	NN
cord-014597-66vd2mdu	1657	36	-	-	NN
cord-014597-66vd2mdu	1657	37	consumption	consumption	NN
cord-014597-66vd2mdu	1657	38	.	.	.
cord-014597-66vd2mdu	1658	1	Some	some	DT
cord-014597-66vd2mdu	1658	2	authors	author	NNS
cord-014597-66vd2mdu	1658	3	hypothesize	hypothesize	VBP
cord-014597-66vd2mdu	1658	4	that	that	IN
cord-014597-66vd2mdu	1658	5	cells	cell	NNS
cord-014597-66vd2mdu	1658	6	metabolize	metabolize	VBP
cord-014597-66vd2mdu	1658	7	extracellular	extracellular	JJ
cord-014597-66vd2mdu	1658	8	lactate	lactate	NN
cord-014597-66vd2mdu	1658	9	as	as	IN
cord-014597-66vd2mdu	1658	10	a	a	DT
cord-014597-66vd2mdu	1658	11	strategy	strategy	NN
cord-014597-66vd2mdu	1658	12	for	for	IN
cord-014597-66vd2mdu	1658	13	pH	pH	NNP
cord-014597-66vd2mdu	1658	14	detoxification	detoxification	NN
cord-014597-66vd2mdu	1658	15	[	[	-LRB-
cord-014597-66vd2mdu	1658	16	2	2	CD
cord-014597-66vd2mdu	1658	17	]	]	-RRB-
cord-014597-66vd2mdu	1658	18	.	.	.
cord-014597-66vd2mdu	1659	1	Glucose	glucose	NN
cord-014597-66vd2mdu	1659	2	and	and	CC
cord-014597-66vd2mdu	1659	3	lactate	lactate	NN
cord-014597-66vd2mdu	1659	4	co	co	NN
cord-014597-66vd2mdu	1659	5	-	-	NN
cord-014597-66vd2mdu	1659	6	metabolization	metabolization	NN
cord-014597-66vd2mdu	1659	7	resulted	result	VBD
cord-014597-66vd2mdu	1659	8	in	in	IN
cord-014597-66vd2mdu	1659	9	a	a	DT
cord-014597-66vd2mdu	1659	10	better	well	RBR
cord-014597-66vd2mdu	1659	11	-	-	HYPH
cord-014597-66vd2mdu	1659	12	balanced	balanced	JJ
cord-014597-66vd2mdu	1659	13	cell	cell	NN
cord-014597-66vd2mdu	1659	14	metabolism	metabolism	NN
cord-014597-66vd2mdu	1659	15	,	,	,
cord-014597-66vd2mdu	1659	16	as	as	IN
cord-014597-66vd2mdu	1659	17	can	can	MD
cord-014597-66vd2mdu	1659	18	be	be	VB
cord-014597-66vd2mdu	1659	19	seen	see	VBN
cord-014597-66vd2mdu	1659	20	from	from	IN
cord-014597-66vd2mdu	1659	21	the	the	DT
cord-014597-66vd2mdu	1659	22	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1659	23	fluxes	flux	NNS
cord-014597-66vd2mdu	1659	24	calculated	calculate	VBN
cord-014597-66vd2mdu	1659	25	,	,	,
cord-014597-66vd2mdu	1659	26	with	with	IN
cord-014597-66vd2mdu	1659	27	minor	minor	JJ
cord-014597-66vd2mdu	1659	28	effects	effect	NNS
cord-014597-66vd2mdu	1659	29	on	on	IN
cord-014597-66vd2mdu	1659	30	cell	cell	NN
cord-014597-66vd2mdu	1659	31	growth	growth	NN
cord-014597-66vd2mdu	1659	32	.	.	.
cord-014597-66vd2mdu	1660	1	The	the	DT
cord-014597-66vd2mdu	1660	2	observation	observation	NN
cord-014597-66vd2mdu	1660	3	of	of	IN
cord-014597-66vd2mdu	1660	4	glucose	glucose	NN
cord-014597-66vd2mdu	1660	5	and	and	CC
cord-014597-66vd2mdu	1660	6	lactate	lactate	NN
cord-014597-66vd2mdu	1660	7	co	co	NN
cord-014597-66vd2mdu	1660	8	-	-	JJ
cord-014597-66vd2mdu	1660	9	consumption	consumption	JJ
cord-014597-66vd2mdu	1660	10	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1660	11	behavior	behavior	NN
cord-014597-66vd2mdu	1660	12	and	and	CC
cord-014597-66vd2mdu	1660	13	its	-PRON-	PRP$
cord-014597-66vd2mdu	1660	14	deeper	deep	JJR
cord-014597-66vd2mdu	1660	15	study	study	NN
cord-014597-66vd2mdu	1660	16	and	and	CC
cord-014597-66vd2mdu	1660	17	characterization	characterization	NN
cord-014597-66vd2mdu	1660	18	could	could	MD
cord-014597-66vd2mdu	1660	19	open	open	VB
cord-014597-66vd2mdu	1660	20	the	the	DT
cord-014597-66vd2mdu	1660	21	door	door	NN
cord-014597-66vd2mdu	1660	22	of	of	IN
cord-014597-66vd2mdu	1660	23	novel	novel	NN
cord-014597-66vd2mdu	1660	24	culturing	culturing	NN
cord-014597-66vd2mdu	1660	25	strategies	strategy	NNS
cord-014597-66vd2mdu	1660	26	with	with	IN
cord-014597-66vd2mdu	1660	27	the	the	DT
cord-014597-66vd2mdu	1660	28	aim	aim	NN
cord-014597-66vd2mdu	1660	29	of	of	IN
cord-014597-66vd2mdu	1660	30	increasing	increase	VBG
cord-014597-66vd2mdu	1660	31	bioprocesses	bioprocesse	NNS
cord-014597-66vd2mdu	1660	32	productivity	productivity	NN
cord-014597-66vd2mdu	1660	33	.	.	.
cord-014597-66vd2mdu	1661	1	Background	Background	NNP
cord-014597-66vd2mdu	1661	2	Transient	transient	JJ
cord-014597-66vd2mdu	1661	3	protein	protein	NN
cord-014597-66vd2mdu	1661	4	expression	expression	NN
cord-014597-66vd2mdu	1661	5	in	in	IN
cord-014597-66vd2mdu	1661	6	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1661	7	cell	cell	NN
cord-014597-66vd2mdu	1661	8	lines	line	NNS
cord-014597-66vd2mdu	1661	9	has	have	VBZ
cord-014597-66vd2mdu	1661	10	gained	gain	VBN
cord-014597-66vd2mdu	1661	11	increasing	increase	VBG
cord-014597-66vd2mdu	1661	12	relevance	relevance	NN
cord-014597-66vd2mdu	1661	13	as	as	IN
cord-014597-66vd2mdu	1661	14	it	-PRON-	PRP
cord-014597-66vd2mdu	1661	15	enables	enable	VBZ
cord-014597-66vd2mdu	1661	16	fast	fast	JJ
cord-014597-66vd2mdu	1661	17	and	and	CC
cord-014597-66vd2mdu	1661	18	flexible	flexible	JJ
cord-014597-66vd2mdu	1661	19	production	production	NN
cord-014597-66vd2mdu	1661	20	of	of	IN
cord-014597-66vd2mdu	1661	21	high	high	JJ
cord-014597-66vd2mdu	1661	22	-	-	HYPH
cord-014597-66vd2mdu	1661	23	quality	quality	NN
cord-014597-66vd2mdu	1661	24	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	1661	25	protein	protein	NN
cord-014597-66vd2mdu	1661	26	.	.	.
cord-014597-66vd2mdu	1662	1	Considerable	considerable	JJ
cord-014597-66vd2mdu	1662	2	efforts	effort	NNS
cord-014597-66vd2mdu	1662	3	have	have	VBP
cord-014597-66vd2mdu	1662	4	thus	thus	RB
cord-014597-66vd2mdu	1662	5	been	be	VBN
cord-014597-66vd2mdu	1662	6	made	make	VBN
cord-014597-66vd2mdu	1662	7	to	to	TO
cord-014597-66vd2mdu	1662	8	overcome	overcome	VB
cord-014597-66vd2mdu	1662	9	existing	exist	VBG
cord-014597-66vd2mdu	1662	10	limiting	limit	VBG
cord-014597-66vd2mdu	1662	11	aspects	aspect	NNS
cord-014597-66vd2mdu	1662	12	of	of	IN
cord-014597-66vd2mdu	1662	13	transient	transient	JJ
cord-014597-66vd2mdu	1662	14	gene	gene	NN
cord-014597-66vd2mdu	1662	15	expression	expression	NN
cord-014597-66vd2mdu	1662	16	systems	system	NNS
cord-014597-66vd2mdu	1662	17	,	,	,
cord-014597-66vd2mdu	1662	18	in	in	IN
cord-014597-66vd2mdu	1662	19	terms	term	NNS
cord-014597-66vd2mdu	1662	20	of	of	IN
cord-014597-66vd2mdu	1662	21	cell	cell	NN
cord-014597-66vd2mdu	1662	22	lines	line	NNS
cord-014597-66vd2mdu	1662	23	,	,	,
cord-014597-66vd2mdu	1662	24	cell	cell	NN
cord-014597-66vd2mdu	1662	25	culture	culture	NN
cord-014597-66vd2mdu	1662	26	-	-	HYPH
cord-014597-66vd2mdu	1662	27	based	base	VBN
cord-014597-66vd2mdu	1662	28	systems	system	NNS
cord-014597-66vd2mdu	1662	29	,	,	,
cord-014597-66vd2mdu	1662	30	and	and	CC
cord-014597-66vd2mdu	1662	31	protein	protein	NN
cord-014597-66vd2mdu	1662	32	production	production	NN
cord-014597-66vd2mdu	1662	33	in	in	IN
cord-014597-66vd2mdu	1662	34	a	a	DT
cord-014597-66vd2mdu	1662	35	cost	cost	NN
cord-014597-66vd2mdu	1662	36	-	-	HYPH
cord-014597-66vd2mdu	1662	37	effective	effective	JJ
cord-014597-66vd2mdu	1662	38	manner	manner	NN
cord-014597-66vd2mdu	1662	39	.	.	.
cord-014597-66vd2mdu	1663	1	Milligram	milligram	NN
cord-014597-66vd2mdu	1663	2	amounts	amount	NNS
cord-014597-66vd2mdu	1663	3	of	of	IN
cord-014597-66vd2mdu	1663	4	protein	protein	NN
cord-014597-66vd2mdu	1663	5	per	per	IN
cord-014597-66vd2mdu	1663	6	liter	liter	NN
cord-014597-66vd2mdu	1663	7	can	can	MD
cord-014597-66vd2mdu	1663	8	be	be	VB
cord-014597-66vd2mdu	1663	9	produced	produce	VBN
cord-014597-66vd2mdu	1663	10	within	within	IN
cord-014597-66vd2mdu	1663	11	several	several	JJ
cord-014597-66vd2mdu	1663	12	days	day	NNS
cord-014597-66vd2mdu	1663	13	,	,	,
cord-014597-66vd2mdu	1663	14	allowing	allow	VBG
cord-014597-66vd2mdu	1663	15	a	a	DT
cord-014597-66vd2mdu	1663	16	significant	significant	JJ
cord-014597-66vd2mdu	1663	17	shortening	shortening	NN
cord-014597-66vd2mdu	1663	18	of	of	IN
cord-014597-66vd2mdu	1663	19	the	the	DT
cord-014597-66vd2mdu	1663	20	bioproduction	bioproduction	NN
cord-014597-66vd2mdu	1663	21	process	process	NN
cord-014597-66vd2mdu	1663	22	in	in	IN
cord-014597-66vd2mdu	1663	23	comparison	comparison	NN
cord-014597-66vd2mdu	1663	24	to	to	IN
cord-014597-66vd2mdu	1663	25	protein	protein	NN
cord-014597-66vd2mdu	1663	26	production	production	NN
cord-014597-66vd2mdu	1663	27	from	from	IN
cord-014597-66vd2mdu	1663	28	stable	stable	JJ
cord-014597-66vd2mdu	1663	29	clones	clone	NNS
cord-014597-66vd2mdu	1663	30	.	.	.
cord-014597-66vd2mdu	1664	1	To	to	TO
cord-014597-66vd2mdu	1664	2	ensure	ensure	VB
cord-014597-66vd2mdu	1664	3	the	the	DT
cord-014597-66vd2mdu	1664	4	robustness	robustness	NN
cord-014597-66vd2mdu	1664	5	of	of	IN
cord-014597-66vd2mdu	1664	6	the	the	DT
cord-014597-66vd2mdu	1664	7	process	process	NN
cord-014597-66vd2mdu	1664	8	,	,	,
cord-014597-66vd2mdu	1664	9	it	-PRON-	PRP
cord-014597-66vd2mdu	1664	10	is	be	VBZ
cord-014597-66vd2mdu	1664	11	essential	essential	JJ
cord-014597-66vd2mdu	1664	12	to	to	TO
cord-014597-66vd2mdu	1664	13	have	have	VB
cord-014597-66vd2mdu	1664	14	a	a	DT
cord-014597-66vd2mdu	1664	15	reliable	reliable	JJ
cord-014597-66vd2mdu	1664	16	and	and	CC
cord-014597-66vd2mdu	1664	17	easy	easy	JJ
cord-014597-66vd2mdu	1664	18	-	-	HYPH
cord-014597-66vd2mdu	1664	19	to	to	IN
cord-014597-66vd2mdu	1664	20	-	-	HYPH
cord-014597-66vd2mdu	1664	21	use	use	NN
cord-014597-66vd2mdu	1664	22	transfection	transfection	NN
cord-014597-66vd2mdu	1664	23	method	method	NN
cord-014597-66vd2mdu	1664	24	.	.	.
cord-014597-66vd2mdu	1665	1	To	to	TO
cord-014597-66vd2mdu	1665	2	palliate	palliate	VB
cord-014597-66vd2mdu	1665	3	for	for	IN
cord-014597-66vd2mdu	1665	4	the	the	DT
cord-014597-66vd2mdu	1665	5	need	need	NN
cord-014597-66vd2mdu	1665	6	of	of	IN
cord-014597-66vd2mdu	1665	7	a	a	DT
cord-014597-66vd2mdu	1665	8	reliable	reliable	JJ
cord-014597-66vd2mdu	1665	9	transfection	transfection	NN
cord-014597-66vd2mdu	1665	10	reagent	reagent	NN
cord-014597-66vd2mdu	1665	11	,	,	,
cord-014597-66vd2mdu	1665	12	we	-PRON-	PRP
cord-014597-66vd2mdu	1665	13	developed	develop	VBD
cord-014597-66vd2mdu	1666	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1666	2	®	®	NNPS
cord-014597-66vd2mdu	1666	3	,	,	,
cord-014597-66vd2mdu	1666	4	the	the	DT
cord-014597-66vd2mdu	1666	5	only	only	JJ
cord-014597-66vd2mdu	1666	6	commercially	commercially	RB
cord-014597-66vd2mdu	1666	7	available	available	JJ
cord-014597-66vd2mdu	1666	8	PEI	PEI	NNP
cord-014597-66vd2mdu	1666	9	optimised	optimise	VBD
cord-014597-66vd2mdu	1666	10	for	for	IN
cord-014597-66vd2mdu	1666	11	mid	mid	JJ
cord-014597-66vd2mdu	1666	12	to	to	IN
cord-014597-66vd2mdu	1666	13	large	large	JJ
cord-014597-66vd2mdu	1666	14	-	-	HYPH
cord-014597-66vd2mdu	1666	15	scale	scale	NN
cord-014597-66vd2mdu	1666	16	transient	transient	JJ
cord-014597-66vd2mdu	1666	17	protein	protein	NN
cord-014597-66vd2mdu	1666	18	production	production	NN
cord-014597-66vd2mdu	1666	19	during	during	IN
cord-014597-66vd2mdu	1666	20	process	process	NN
cord-014597-66vd2mdu	1666	21	development	development	NN
cord-014597-66vd2mdu	1666	22	.	.	.
cord-014597-66vd2mdu	1667	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1668	1	®	®	NNP
cord-014597-66vd2mdu	1668	2	is	be	VBZ
cord-014597-66vd2mdu	1668	3	a	a	DT
cord-014597-66vd2mdu	1668	4	non	non	JJ
cord-014597-66vd2mdu	1668	5	-	-	JJ
cord-014597-66vd2mdu	1668	6	polydiperse	polydiperse	JJ
cord-014597-66vd2mdu	1668	7	and	and	CC
cord-014597-66vd2mdu	1668	8	fully	fully	RB
cord-014597-66vd2mdu	1668	9	-	-	HYPH
cord-014597-66vd2mdu	1668	10	characterised	characterise	VBN
cord-014597-66vd2mdu	1668	11	polymer	polymer	NN
cord-014597-66vd2mdu	1668	12	that	that	WDT
cord-014597-66vd2mdu	1668	13	has	have	VBZ
cord-014597-66vd2mdu	1668	14	become	become	VBN
cord-014597-66vd2mdu	1668	15	the	the	DT
cord-014597-66vd2mdu	1668	16	gold	gold	NN
cord-014597-66vd2mdu	1668	17	PEI	PEI	NNP
cord-014597-66vd2mdu	1668	18	standard	standard	NN
cord-014597-66vd2mdu	1668	19	due	due	IN
cord-014597-66vd2mdu	1668	20	to	to	IN
cord-014597-66vd2mdu	1668	21	its	-PRON-	PRP$
cord-014597-66vd2mdu	1668	22	reliability	reliability	NN
cord-014597-66vd2mdu	1668	23	,	,	,
cord-014597-66vd2mdu	1668	24	reproducibility	reproducibility	NN
cord-014597-66vd2mdu	1668	25	in	in	IN
cord-014597-66vd2mdu	1668	26	high	high	JJ
cord-014597-66vd2mdu	1668	27	DNA	dna	NN
cord-014597-66vd2mdu	1668	28	delivery	delivery	NN
cord-014597-66vd2mdu	1668	29	efficiency	efficiency	NN
cord-014597-66vd2mdu	1668	30	and	and	CC
cord-014597-66vd2mdu	1668	31	in	in	IN
cord-014597-66vd2mdu	1668	32	ensuing	ensue	VBG
cord-014597-66vd2mdu	1668	33	high	high	JJ
cord-014597-66vd2mdu	1668	34	protein	protein	NN
cord-014597-66vd2mdu	1668	35	production	production	NN
cord-014597-66vd2mdu	1668	36	yields	yield	NNS
cord-014597-66vd2mdu	1668	37	.	.	.
cord-014597-66vd2mdu	1669	1	Here	here	RB
cord-014597-66vd2mdu	1669	2	,	,	,
cord-014597-66vd2mdu	1669	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1669	4	present	present	VBP
cord-014597-66vd2mdu	1669	5	experimental	experimental	JJ
cord-014597-66vd2mdu	1669	6	data	datum	NNS
cord-014597-66vd2mdu	1669	7	showing	show	VBG
cord-014597-66vd2mdu	1669	8	the	the	DT
cord-014597-66vd2mdu	1669	9	benefits	benefit	NNS
cord-014597-66vd2mdu	1669	10	of	of	IN
cord-014597-66vd2mdu	1669	11	using	use	VBG
cord-014597-66vd2mdu	1669	12	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1669	13	®	®	NNP
cord-014597-66vd2mdu	1669	14	for	for	IN
cord-014597-66vd2mdu	1669	15	protein	protein	NN
cord-014597-66vd2mdu	1669	16	production	production	NN
cord-014597-66vd2mdu	1669	17	in	in	IN
cord-014597-66vd2mdu	1669	18	comparison	comparison	NN
cord-014597-66vd2mdu	1669	19	to	to	IN
cord-014597-66vd2mdu	1669	20	other	other	JJ
cord-014597-66vd2mdu	1669	21	PEIs	pei	NNS
cord-014597-66vd2mdu	1669	22	.	.	.
cord-014597-66vd2mdu	1670	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1670	2	further	further	RB
cord-014597-66vd2mdu	1670	3	demonstrate	demonstrate	VBP
cord-014597-66vd2mdu	1670	4	compatibility	compatibility	NN
cord-014597-66vd2mdu	1670	5	of	of	IN
cord-014597-66vd2mdu	1670	6	using	use	VBG
cord-014597-66vd2mdu	1670	7	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1670	8	®	®	NNP
cord-014597-66vd2mdu	1670	9	for	for	IN
cord-014597-66vd2mdu	1670	10	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1670	11	protein	protein	NN
cord-014597-66vd2mdu	1670	12	production	production	NN
cord-014597-66vd2mdu	1670	13	in	in	IN
cord-014597-66vd2mdu	1670	14	most	most	RBS
cord-014597-66vd2mdu	1670	15	commonly	commonly	RB
cord-014597-66vd2mdu	1670	16	used	use	VBN
cord-014597-66vd2mdu	1670	17	chemicallydefined	chemicallydefined	JJ
cord-014597-66vd2mdu	1670	18	media	medium	NNS
cord-014597-66vd2mdu	1670	19	.	.	.
cord-014597-66vd2mdu	1671	1	Materiel	materiel	NN
cord-014597-66vd2mdu	1671	2	and	and	CC
cord-014597-66vd2mdu	1671	3	methods	method	NNS
cord-014597-66vd2mdu	1671	4	Suspension	Suspension	NNP
cord-014597-66vd2mdu	1671	5	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1671	6	and	and	CC
cord-014597-66vd2mdu	1671	7	CHO	CHO	NNP
cord-014597-66vd2mdu	1671	8	cells	cell	NNS
cord-014597-66vd2mdu	1671	9	were	be	VBD
cord-014597-66vd2mdu	1671	10	cultured	culture	VBN
cord-014597-66vd2mdu	1671	11	in	in	IN
cord-014597-66vd2mdu	1671	12	shaker	shaker	NN
cord-014597-66vd2mdu	1671	13	flasks	flask	NNS
cord-014597-66vd2mdu	1671	14	in	in	IN
cord-014597-66vd2mdu	1671	15	various	various	JJ
cord-014597-66vd2mdu	1671	16	synthetic	synthetic	JJ
cord-014597-66vd2mdu	1671	17	media	medium	NNS
cord-014597-66vd2mdu	1671	18	,	,	,
cord-014597-66vd2mdu	1671	19	as	as	IN
cord-014597-66vd2mdu	1671	20	listed	list	VBN
cord-014597-66vd2mdu	1671	21	in	in	IN
cord-014597-66vd2mdu	1671	22	Table	Table	NNP
cord-014597-66vd2mdu	1671	23	1	1	CD
cord-014597-66vd2mdu	1671	24	.	.	.
cord-014597-66vd2mdu	1672	1	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1673	1	and	and	CC
cord-014597-66vd2mdu	1673	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1673	3	cells	cell	NNS
cord-014597-66vd2mdu	1673	4	were	be	VBD
cord-014597-66vd2mdu	1673	5	resuspended	resuspend	VBN
cord-014597-66vd2mdu	1673	6	at	at	IN
cord-014597-66vd2mdu	1673	7	1×10	1×10	CD
cord-014597-66vd2mdu	1673	8	6	6	CD
cord-014597-66vd2mdu	1673	9	cells	cell	NNS
cord-014597-66vd2mdu	1673	10	/	/	SYM
cord-014597-66vd2mdu	1673	11	ml	ml	NNS
cord-014597-66vd2mdu	1673	12	of	of	IN
cord-014597-66vd2mdu	1673	13	serum	serum	NN
cord-014597-66vd2mdu	1673	14	-	-	HYPH
cord-014597-66vd2mdu	1673	15	free	free	JJ
cord-014597-66vd2mdu	1673	16	medium	medium	NN
cord-014597-66vd2mdu	1673	17	,	,	,
cord-014597-66vd2mdu	1673	18	on	on	IN
cord-014597-66vd2mdu	1673	19	the	the	DT
cord-014597-66vd2mdu	1673	20	day	day	NN
cord-014597-66vd2mdu	1673	21	before	before	IN
cord-014597-66vd2mdu	1673	22	transfection	transfection	NN
cord-014597-66vd2mdu	1673	23	.	.	.
cord-014597-66vd2mdu	1674	1	Cells	cell	NNS
cord-014597-66vd2mdu	1674	2	were	be	VBD
cord-014597-66vd2mdu	1674	3	transfected	transfecte	VBN
cord-014597-66vd2mdu	1674	4	with	with	IN
cord-014597-66vd2mdu	1674	5	0.5	0.5	CD
cord-014597-66vd2mdu	1674	6	-	-	SYM
cord-014597-66vd2mdu	1674	7	1	1	CD
cord-014597-66vd2mdu	1674	8	mg	mg	NN
cord-014597-66vd2mdu	1674	9	of	of	IN
cord-014597-66vd2mdu	1674	10	plasmid	plasmid	NN
cord-014597-66vd2mdu	1674	11	DNA	dna	NN
cord-014597-66vd2mdu	1674	12	encoding	encoding	NN
cord-014597-66vd2mdu	1674	13	for	for	IN
cord-014597-66vd2mdu	1674	14	the	the	DT
cord-014597-66vd2mdu	1674	15	Luciferase	Luciferase	NNP
cord-014597-66vd2mdu	1674	16	gene	gene	NN
cord-014597-66vd2mdu	1674	17	reporter	reporter	NN
cord-014597-66vd2mdu	1674	18	using	use	VBG
cord-014597-66vd2mdu	1674	19	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1674	20	®	®	NNPS
cord-014597-66vd2mdu	1674	21	,	,	,
cord-014597-66vd2mdu	1674	22	PEI	PEI	NNP
cord-014597-66vd2mdu	1674	23	"	"	``
cord-014597-66vd2mdu	1674	24	Max	Max	NNP
cord-014597-66vd2mdu	1674	25	"	"	''
cord-014597-66vd2mdu	1674	26	and	and	CC
cord-014597-66vd2mdu	1674	27	L	L	NNP
cord-014597-66vd2mdu	1674	28	-	-	HYPH
cord-014597-66vd2mdu	1674	29	PEI	PEI	NNP
cord-014597-66vd2mdu	1674	30	25	25	CD
cord-014597-66vd2mdu	1674	31	kDa	kDa	NNS
cord-014597-66vd2mdu	1675	1	(	(	-LRB-
cord-014597-66vd2mdu	1675	2	Polysciences	Polysciences	NNP
cord-014597-66vd2mdu	1675	3	,	,	,
cord-014597-66vd2mdu	1675	4	Warrington	Warrington	NNP
cord-014597-66vd2mdu	1675	5	,	,	,
cord-014597-66vd2mdu	1675	6	PA	PA	NNP
cord-014597-66vd2mdu	1675	7	)	)	-RRB-
cord-014597-66vd2mdu	1675	8	resuspended	resuspend	VBD
cord-014597-66vd2mdu	1675	9	at	at	IN
cord-014597-66vd2mdu	1675	10	1	1	CD
cord-014597-66vd2mdu	1675	11	mg	mg	NNP
cord-014597-66vd2mdu	1675	12	/	/	SYM
cord-014597-66vd2mdu	1675	13	ml	ml	NN
cord-014597-66vd2mdu	1675	14	according	accord	VBG
cord-014597-66vd2mdu	1675	15	to	to	IN
cord-014597-66vd2mdu	1675	16	the	the	DT
cord-014597-66vd2mdu	1675	17	manufacturer	manufacturer	NN
cord-014597-66vd2mdu	1675	18	's	's	POS
cord-014597-66vd2mdu	1675	19	recommendation	recommendation	NN
cord-014597-66vd2mdu	1675	20	.	.	.
cord-014597-66vd2mdu	1676	1	Protein	protein	NN
cord-014597-66vd2mdu	1676	2	expression	expression	NN
cord-014597-66vd2mdu	1676	3	of	of	IN
cord-014597-66vd2mdu	1676	4	the	the	DT
cord-014597-66vd2mdu	1676	5	luciferase	luciferase	NN
cord-014597-66vd2mdu	1676	6	reporter	reporter	NN
cord-014597-66vd2mdu	1676	7	gene	gene	NN
cord-014597-66vd2mdu	1676	8	was	be	VBD
cord-014597-66vd2mdu	1676	9	assayed	assay	VBN
cord-014597-66vd2mdu	1676	10	48	48	CD
cord-014597-66vd2mdu	1676	11	hours	hour	NNS
cord-014597-66vd2mdu	1676	12	post	post	NN
cord-014597-66vd2mdu	1676	13	-	-	NN
cord-014597-66vd2mdu	1676	14	transfection	transfection	NN
cord-014597-66vd2mdu	1676	15	by	by	IN
cord-014597-66vd2mdu	1676	16	affinity	affinity	NN
cord-014597-66vd2mdu	1676	17	chromatography	chromatography	NN
cord-014597-66vd2mdu	1676	18	using	use	VBG
cord-014597-66vd2mdu	1676	19	protein	protein	NN
cord-014597-66vd2mdu	1676	20	G	G	NNP
cord-014597-66vd2mdu	1676	21	(	(	-LRB-
cord-014597-66vd2mdu	1676	22	HPLC	HPLC	NNP
cord-014597-66vd2mdu	1676	23	)	)	-RRB-
cord-014597-66vd2mdu	1676	24	.	.	.
cord-014597-66vd2mdu	1677	1	Comparison	comparison	NN
cord-014597-66vd2mdu	1677	2	of	of	IN
cord-014597-66vd2mdu	1677	3	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1678	1	®	®	VB
cord-014597-66vd2mdu	1678	2	to	to	IN
cord-014597-66vd2mdu	1678	3	other	other	JJ
cord-014597-66vd2mdu	1678	4	commercially	commercially	RB
cord-014597-66vd2mdu	1678	5	available	available	JJ
cord-014597-66vd2mdu	1678	6	PEIs	pei	NNS
cord-014597-66vd2mdu	1678	7	was	be	VBD
cord-014597-66vd2mdu	1678	8	achieved	achieve	VBN
cord-014597-66vd2mdu	1678	9	by	by	IN
cord-014597-66vd2mdu	1678	10	transfecting	transfecte	VBG
cord-014597-66vd2mdu	1678	11	suspension	suspension	NN
cord-014597-66vd2mdu	1679	1	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1679	2	and	and	CC
cord-014597-66vd2mdu	1679	3	CHO	CHO	NNP
cord-014597-66vd2mdu	1679	4	cells	cell	NNS
cord-014597-66vd2mdu	1679	5	with	with	IN
cord-014597-66vd2mdu	1679	6	plasmid	plasmid	NN
cord-014597-66vd2mdu	1679	7	DNA	dna	NN
cord-014597-66vd2mdu	1679	8	encoding	encoding	NN
cord-014597-66vd2mdu	1679	9	for	for	IN
cord-014597-66vd2mdu	1679	10	the	the	DT
cord-014597-66vd2mdu	1679	11	luciferase	luciferase	NN
cord-014597-66vd2mdu	1679	12	gene	gene	NN
cord-014597-66vd2mdu	1679	13	reporter	reporter	NN
cord-014597-66vd2mdu	1679	14	.	.	.
cord-014597-66vd2mdu	1680	1	Luciferase	luciferase	NN
cord-014597-66vd2mdu	1680	2	production	production	NN
cord-014597-66vd2mdu	1680	3	yields	yield	NNS
cord-014597-66vd2mdu	1680	4	obtained	obtain	VBN
cord-014597-66vd2mdu	1680	5	in	in	IN
cord-014597-66vd2mdu	1680	6	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1680	7	and	and	CC
cord-014597-66vd2mdu	1680	8	CHO	CHO	NNP
cord-014597-66vd2mdu	1680	9	cells	cell	NNS
cord-014597-66vd2mdu	1680	10	were	be	VBD
cord-014597-66vd2mdu	1680	11	at	at	IN
cord-014597-66vd2mdu	1680	12	least	least	JJS
cord-014597-66vd2mdu	1680	13	respectively	respectively	RB
cord-014597-66vd2mdu	1680	14	5-fold	5-fold	CD
cord-014597-66vd2mdu	1680	15	and	and	CC
cord-014597-66vd2mdu	1680	16	10-fold	10-fold	JJ
cord-014597-66vd2mdu	1680	17	higher	high	JJR
cord-014597-66vd2mdu	1680	18	when	when	WRB
cord-014597-66vd2mdu	1680	19	using	use	VBG
cord-014597-66vd2mdu	1680	20	a	a	DT
cord-014597-66vd2mdu	1680	21	similar	similar	JJ
cord-014597-66vd2mdu	1680	22	amount	amount	NN
cord-014597-66vd2mdu	1680	23	of	of	IN
cord-014597-66vd2mdu	1680	24	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1680	25	in	in	IN
cord-014597-66vd2mdu	1680	26	comparison	comparison	NN
cord-014597-66vd2mdu	1680	27	to	to	IN
cord-014597-66vd2mdu	1680	28	the	the	DT
cord-014597-66vd2mdu	1680	29	other	other	JJ
cord-014597-66vd2mdu	1680	30	PEIs	pei	NNS
cord-014597-66vd2mdu	1680	31	(	(	-LRB-
cord-014597-66vd2mdu	1680	32	Fig	fig	NN
cord-014597-66vd2mdu	1680	33	.	.	.
cord-014597-66vd2mdu	1680	34	1	1	CD
cord-014597-66vd2mdu	1680	35	)	)	-RRB-
cord-014597-66vd2mdu	1680	36	.	.	.
cord-014597-66vd2mdu	1681	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1681	2	,	,	,
cord-014597-66vd2mdu	1681	3	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1681	4	®	®	NNP
cord-014597-66vd2mdu	1681	5	was	be	VBD
cord-014597-66vd2mdu	1681	6	the	the	DT
cord-014597-66vd2mdu	1681	7	only	only	JJ
cord-014597-66vd2mdu	1681	8	PEI	PEI	NNP
cord-014597-66vd2mdu	1681	9	that	that	WDT
cord-014597-66vd2mdu	1681	10	led	lead	VBD
cord-014597-66vd2mdu	1681	11	to	to	IN
cord-014597-66vd2mdu	1681	12	similar	similar	JJ
cord-014597-66vd2mdu	1681	13	luciferase	luciferase	NN
cord-014597-66vd2mdu	1681	14	production	production	NN
cord-014597-66vd2mdu	1681	15	yields	yield	NNS
cord-014597-66vd2mdu	1681	16	when	when	WRB
cord-014597-66vd2mdu	1681	17	decreasing	decrease	VBG
cord-014597-66vd2mdu	1681	18	the	the	DT
cord-014597-66vd2mdu	1681	19	amount	amount	NN
cord-014597-66vd2mdu	1681	20	of	of	IN
cord-014597-66vd2mdu	1681	21	plasmid	plasmid	NN
cord-014597-66vd2mdu	1681	22	DNA	dna	NN
cord-014597-66vd2mdu	1681	23	per	per	IN
cord-014597-66vd2mdu	1681	24	Liter	liter	NN
cord-014597-66vd2mdu	1681	25	of	of	IN
cord-014597-66vd2mdu	1681	26	cell	cell	NN
cord-014597-66vd2mdu	1681	27	culture	culture	NN
cord-014597-66vd2mdu	1681	28	.	.	.
cord-014597-66vd2mdu	1682	1	Conversely	conversely	RB
cord-014597-66vd2mdu	1682	2	,	,	,
cord-014597-66vd2mdu	1682	3	at	at	IN
cord-014597-66vd2mdu	1682	4	least	least	JJS
cord-014597-66vd2mdu	1682	5	1	1	CD
cord-014597-66vd2mdu	1682	6	mg	mg	NN
cord-014597-66vd2mdu	1682	7	of	of	IN
cord-014597-66vd2mdu	1682	8	plasmid	plasmid	NN
cord-014597-66vd2mdu	1682	9	DNA	dna	NN
cord-014597-66vd2mdu	1682	10	and	and	CC
cord-014597-66vd2mdu	1682	11	4-fold	4-fold	CD
cord-014597-66vd2mdu	1682	12	more	more	JJR
cord-014597-66vd2mdu	1682	13	of	of	IN
cord-014597-66vd2mdu	1682	14	PEI	PEI	NNP
cord-014597-66vd2mdu	1682	15	"	"	``
cord-014597-66vd2mdu	1682	16	Max	Max	NNP
cord-014597-66vd2mdu	1682	17	"	"	''
cord-014597-66vd2mdu	1682	18	and	and	CC
cord-014597-66vd2mdu	1682	19	L	L	NNP
cord-014597-66vd2mdu	1682	20	-	-	HYPH
cord-014597-66vd2mdu	1682	21	PEI	PEI	NNP
cord-014597-66vd2mdu	1682	22	25	25	CD
cord-014597-66vd2mdu	1682	23	kDa	kDa	NNPS
cord-014597-66vd2mdu	1682	24	were	be	VBD
cord-014597-66vd2mdu	1682	25	needed	need	VBN
cord-014597-66vd2mdu	1682	26	to	to	TO
cord-014597-66vd2mdu	1682	27	obtain	obtain	VB
cord-014597-66vd2mdu	1682	28	a	a	DT
cord-014597-66vd2mdu	1682	29	similar	similar	JJ
cord-014597-66vd2mdu	1682	30	Luciferase	Luciferase	NNP
cord-014597-66vd2mdu	1682	31	expression	expression	NN
cord-014597-66vd2mdu	1682	32	range	range	NN
cord-014597-66vd2mdu	1682	33	in	in	IN
cord-014597-66vd2mdu	1682	34	both	both	CC
cord-014597-66vd2mdu	1682	35	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1682	36	and	and	CC
cord-014597-66vd2mdu	1682	37	CHO	CHO	NNP
cord-014597-66vd2mdu	1682	38	cells	cell	NNS
cord-014597-66vd2mdu	1682	39	.	.	.
cord-014597-66vd2mdu	1683	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1683	2	further	further	RB
cord-014597-66vd2mdu	1683	3	assessed	assess	VBD
cord-014597-66vd2mdu	1683	4	the	the	DT
cord-014597-66vd2mdu	1683	5	compatibility	compatibility	NN
cord-014597-66vd2mdu	1683	6	and	and	CC
cord-014597-66vd2mdu	1683	7	versatility	versatility	NN
cord-014597-66vd2mdu	1683	8	of	of	IN
cord-014597-66vd2mdu	1683	9	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1684	1	®	®	VBN
cord-014597-66vd2mdu	1684	2	by	by	IN
cord-014597-66vd2mdu	1684	3	measuring	measure	VBG
cord-014597-66vd2mdu	1684	4	protein	protein	NN
cord-014597-66vd2mdu	1684	5	production	production	NN
cord-014597-66vd2mdu	1684	6	yields	yield	NNS
cord-014597-66vd2mdu	1684	7	obtained	obtain	VBN
cord-014597-66vd2mdu	1684	8	in	in	IN
cord-014597-66vd2mdu	1684	9	most	most	RBS
cord-014597-66vd2mdu	1684	10	commonly	commonly	RB
cord-014597-66vd2mdu	1684	11	used	use	VBN
cord-014597-66vd2mdu	1684	12	animal	animal	NN
cord-014597-66vd2mdu	1684	13	-	-	HYPH
cord-014597-66vd2mdu	1684	14	free	free	JJ
cord-014597-66vd2mdu	1684	15	synthetic	synthetic	JJ
cord-014597-66vd2mdu	1684	16	media	medium	NNS
cord-014597-66vd2mdu	1684	17	.	.	.
cord-014597-66vd2mdu	1685	1	As	as	IN
cord-014597-66vd2mdu	1685	2	shown	show	VBN
cord-014597-66vd2mdu	1685	3	in	in	IN
cord-014597-66vd2mdu	1685	4	Fig	Fig	NNP
cord-014597-66vd2mdu	1685	5	.	.	.
cord-014597-66vd2mdu	1686	1	2	2	LS
cord-014597-66vd2mdu	1686	2	,	,	,
cord-014597-66vd2mdu	1686	3	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1686	4	®	®	NNP
cord-014597-66vd2mdu	1686	5	leads	lead	NNS
cord-014597-66vd2mdu	1686	6	to	to	IN
cord-014597-66vd2mdu	1686	7	high	high	JJ
cord-014597-66vd2mdu	1686	8	protein	protein	NN
cord-014597-66vd2mdu	1686	9	production	production	NN
cord-014597-66vd2mdu	1686	10	yieds	yied	NNS
cord-014597-66vd2mdu	1686	11	in	in	IN
cord-014597-66vd2mdu	1686	12	several	several	JJ
cord-014597-66vd2mdu	1686	13	commercially	commercially	RB
cord-014597-66vd2mdu	1686	14	avaialble	avaialble	JJ
cord-014597-66vd2mdu	1686	15	media	medium	NNS
cord-014597-66vd2mdu	1686	16	formulations	formulation	NNS
cord-014597-66vd2mdu	1686	17	for	for	IN
cord-014597-66vd2mdu	1686	18	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1686	19	anc	anc	NNP
cord-014597-66vd2mdu	1686	20	CHO	CHO	NNP
cord-014597-66vd2mdu	1686	21	cell	cell	NN
cord-014597-66vd2mdu	1686	22	lines	line	NNS
cord-014597-66vd2mdu	1686	23	.	.	.
cord-014597-66vd2mdu	1687	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1688	1	®	®	NNP
cord-014597-66vd2mdu	1688	2	is	be	VBZ
cord-014597-66vd2mdu	1688	3	the	the	DT
cord-014597-66vd2mdu	1688	4	only	only	JJ
cord-014597-66vd2mdu	1688	5	fully	fully	RB
cord-014597-66vd2mdu	1688	6	characterised	characterise	VBN
cord-014597-66vd2mdu	1688	7	PEI	PEI	NNP
cord-014597-66vd2mdu	1688	8	transfection	transfection	NN
cord-014597-66vd2mdu	1688	9	reagent	reagent	NN
cord-014597-66vd2mdu	1688	10	that	that	WDT
cord-014597-66vd2mdu	1688	11	is	be	VBZ
cord-014597-66vd2mdu	1688	12	suitable	suitable	JJ
cord-014597-66vd2mdu	1688	13	for	for	IN
cord-014597-66vd2mdu	1688	14	reliable	reliable	JJ
cord-014597-66vd2mdu	1688	15	and	and	CC
cord-014597-66vd2mdu	1688	16	reproducible	reproducible	JJ
cord-014597-66vd2mdu	1688	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1688	18	protein	protein	NN
cord-014597-66vd2mdu	1688	19	production	production	NN
cord-014597-66vd2mdu	1688	20	,	,	,
cord-014597-66vd2mdu	1688	21	irrespective	irrespective	RB
cord-014597-66vd2mdu	1688	22	of	of	IN
cord-014597-66vd2mdu	1688	23	the	the	DT
cord-014597-66vd2mdu	1688	24	scale	scale	NN
cord-014597-66vd2mdu	1688	25	of	of	IN
cord-014597-66vd2mdu	1688	26	production	production	NN
cord-014597-66vd2mdu	1688	27	and	and	CC
cord-014597-66vd2mdu	1688	28	of	of	IN
cord-014597-66vd2mdu	1688	29	the	the	DT
cord-014597-66vd2mdu	1688	30	type	type	NN
cord-014597-66vd2mdu	1688	31	of	of	IN
cord-014597-66vd2mdu	1688	32	adherent	adherent	NN
cord-014597-66vd2mdu	1688	33	and	and	CC
cord-014597-66vd2mdu	1688	34	suspension	suspension	NN
cord-014597-66vd2mdu	1688	35	cell	cell	NN
cord-014597-66vd2mdu	1688	36	culture	culture	NN
cord-014597-66vd2mdu	1688	37	system	system	NN
cord-014597-66vd2mdu	1688	38	.	.	.
cord-014597-66vd2mdu	1689	1	Fig	Fig	NNP
cord-014597-66vd2mdu	1689	2	.	.	.
cord-014597-66vd2mdu	1689	3	1	1	CD
cord-014597-66vd2mdu	1689	4	(	(	-LRB-
cord-014597-66vd2mdu	1689	5	abstract	abstract	JJ
cord-014597-66vd2mdu	1689	6	P-274	p-274	NN
cord-014597-66vd2mdu	1689	7	)	)	-RRB-
cord-014597-66vd2mdu	1689	8	.	.	.
cord-014597-66vd2mdu	1690	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1690	2	®	®	NNP
cord-014597-66vd2mdu	1690	3	requires	require	VBZ
cord-014597-66vd2mdu	1690	4	less	less	RBR
cord-014597-66vd2mdu	1690	5	reagent	reagent	JJ
cord-014597-66vd2mdu	1690	6	and	and	CC
cord-014597-66vd2mdu	1690	7	similar	similar	JJ
cord-014597-66vd2mdu	1690	8	to	to	IN
cord-014597-66vd2mdu	1690	9	lower	low	JJR
cord-014597-66vd2mdu	1690	10	DNA	dna	NN
cord-014597-66vd2mdu	1690	11	amount	amount	NN
cord-014597-66vd2mdu	1690	12	compared	compare	VBN
cord-014597-66vd2mdu	1690	13	to	to	IN
cord-014597-66vd2mdu	1690	14	other	other	JJ
cord-014597-66vd2mdu	1690	15	PEIs	pei	NNS
cord-014597-66vd2mdu	1690	16	.	.	.
cord-014597-66vd2mdu	1691	1	Suspension	suspension	NN
cord-014597-66vd2mdu	1691	2	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1691	3	and	and	CC
cord-014597-66vd2mdu	1691	4	CHO	CHO	NNP
cord-014597-66vd2mdu	1691	5	cells	cell	NNS
cord-014597-66vd2mdu	1691	6	were	be	VBD
cord-014597-66vd2mdu	1691	7	seeded	seed	VBN
cord-014597-66vd2mdu	1691	8	at	at	IN
cord-014597-66vd2mdu	1691	9	1×10	1×10	CD
cord-014597-66vd2mdu	1691	10	6	6	CD
cord-014597-66vd2mdu	1691	11	cells	cell	NNS
cord-014597-66vd2mdu	1691	12	/	/	SYM
cord-014597-66vd2mdu	1691	13	mL	ml	NN
cord-014597-66vd2mdu	1691	14	in	in	IN
cord-014597-66vd2mdu	1691	15	serum	serum	NN
cord-014597-66vd2mdu	1691	16	free	free	JJ
cord-014597-66vd2mdu	1691	17	medium	medium	NN
cord-014597-66vd2mdu	1691	18	and	and	CC
cord-014597-66vd2mdu	1691	19	transfected	transfecte	VBN
cord-014597-66vd2mdu	1691	20	with	with	IN
cord-014597-66vd2mdu	1691	21	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1691	22	®	®	NNPS
cord-014597-66vd2mdu	1691	23	,	,	,
cord-014597-66vd2mdu	1691	24	PEI	PEI	NNP
cord-014597-66vd2mdu	1691	25	"	"	``
cord-014597-66vd2mdu	1691	26	Max	Max	NNP
cord-014597-66vd2mdu	1691	27	"	"	''
cord-014597-66vd2mdu	1691	28	and	and	CC
cord-014597-66vd2mdu	1691	29	L	L	NNP
cord-014597-66vd2mdu	1691	30	-	-	HYPH
cord-014597-66vd2mdu	1691	31	PEI	PEI	NNP
cord-014597-66vd2mdu	1691	32	25	25	CD
cord-014597-66vd2mdu	1691	33	kDa	kDa	NNS
cord-014597-66vd2mdu	1692	1	(	(	-LRB-
cord-014597-66vd2mdu	1692	2	Polysciences	Polysciences	NNP
cord-014597-66vd2mdu	1692	3	,	,	,
cord-014597-66vd2mdu	1692	4	Warrington	Warrington	NNP
cord-014597-66vd2mdu	1692	5	,	,	,
cord-014597-66vd2mdu	1692	6	PA	PA	NNP
cord-014597-66vd2mdu	1692	7	)	)	-RRB-
cord-014597-66vd2mdu	1692	8	resuspended	resuspend	VBD
cord-014597-66vd2mdu	1692	9	at	at	IN
cord-014597-66vd2mdu	1692	10	1	1	CD
cord-014597-66vd2mdu	1692	11	mg	mg	NNP
cord-014597-66vd2mdu	1692	12	/	/	SYM
cord-014597-66vd2mdu	1692	13	ml	ml	NN
cord-014597-66vd2mdu	1692	14	.	.	.
cord-014597-66vd2mdu	1693	1	Luciferase	Luciferase	NNP
cord-014597-66vd2mdu	1693	2	expression	expression	NN
cord-014597-66vd2mdu	1693	3	was	be	VBD
cord-014597-66vd2mdu	1693	4	assayed	assay	VBN
cord-014597-66vd2mdu	1693	5	48	48	CD
cord-014597-66vd2mdu	1693	6	h	h	NN
cord-014597-66vd2mdu	1693	7	after	after	IN
cord-014597-66vd2mdu	1693	8	transfection	transfection	NN
cord-014597-66vd2mdu	1693	9	using	use	VBG
cord-014597-66vd2mdu	1693	10	a	a	DT
cord-014597-66vd2mdu	1693	11	conventional	conventional	JJ
cord-014597-66vd2mdu	1693	12	luciferase	luciferase	NN
cord-014597-66vd2mdu	1693	13	assay	assay	NN
cord-014597-66vd2mdu	1693	14	Fig	Fig	NNP
cord-014597-66vd2mdu	1693	15	.	.	.
cord-014597-66vd2mdu	1693	16	2	2	LS
cord-014597-66vd2mdu	1693	17	(	(	-LRB-
cord-014597-66vd2mdu	1693	18	abstract	abstract	JJ
cord-014597-66vd2mdu	1693	19	P-274	p-274	NN
cord-014597-66vd2mdu	1693	20	)	)	-RRB-
cord-014597-66vd2mdu	1693	21	.	.	.
cord-014597-66vd2mdu	1694	1	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1695	1	®	®	NNP
cord-014597-66vd2mdu	1695	2	is	be	VBZ
cord-014597-66vd2mdu	1695	3	optimized	optimize	VBN
cord-014597-66vd2mdu	1695	4	for	for	IN
cord-014597-66vd2mdu	1695	5	transfection	transfection	NN
cord-014597-66vd2mdu	1695	6	of	of	IN
cord-014597-66vd2mdu	1695	7	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1695	8	and	and	CC
cord-014597-66vd2mdu	1695	9	CHO	CHO	NNP
cord-014597-66vd2mdu	1695	10	cells	cell	NNS
cord-014597-66vd2mdu	1695	11	in	in	IN
cord-014597-66vd2mdu	1695	12	several	several	JJ
cord-014597-66vd2mdu	1695	13	specific	specific	JJ
cord-014597-66vd2mdu	1695	14	synthetic	synthetic	JJ
cord-014597-66vd2mdu	1695	15	culture	culture	NN
cord-014597-66vd2mdu	1695	16	media	medium	NNS
cord-014597-66vd2mdu	1695	17	.	.	.
cord-014597-66vd2mdu	1696	1	Suspension	suspension	NN
cord-014597-66vd2mdu	1696	2	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1696	3	and	and	CC
cord-014597-66vd2mdu	1696	4	CHO	CHO	NNP
cord-014597-66vd2mdu	1696	5	cells	cell	NNS
cord-014597-66vd2mdu	1696	6	were	be	VBD
cord-014597-66vd2mdu	1696	7	seeded	seed	VBN
cord-014597-66vd2mdu	1696	8	following	follow	VBG
cord-014597-66vd2mdu	1696	9	the	the	DT
cord-014597-66vd2mdu	1696	10	recommended	recommend	VBN
cord-014597-66vd2mdu	1696	11	protocol	protocol	NN
cord-014597-66vd2mdu	1696	12	in	in	IN
cord-014597-66vd2mdu	1696	13	serum	serum	NN
cord-014597-66vd2mdu	1696	14	-	-	HYPH
cord-014597-66vd2mdu	1696	15	free	free	JJ
cord-014597-66vd2mdu	1696	16	media	medium	NNS
cord-014597-66vd2mdu	1696	17	and	and	CC
cord-014597-66vd2mdu	1696	18	transfected	transfecte	VBN
cord-014597-66vd2mdu	1696	19	with	with	IN
cord-014597-66vd2mdu	1696	20	PEIpro	PEIpro	NNP
cord-014597-66vd2mdu	1697	1	®	®	VB
cord-014597-66vd2mdu	1697	2	using	use	VBG
cord-014597-66vd2mdu	1697	3	the	the	DT
cord-014597-66vd2mdu	1697	4	standard	standard	JJ
cord-014597-66vd2mdu	1697	5	conditions	condition	NNS
cord-014597-66vd2mdu	1697	6	.	.	.
cord-014597-66vd2mdu	1698	1	IgG3-Fc	IgG3-Fc	NNP
cord-014597-66vd2mdu	1699	1	production	production	NN
cord-014597-66vd2mdu	1699	2	was	be	VBD
cord-014597-66vd2mdu	1699	3	assayed	assay	VBN
cord-014597-66vd2mdu	1699	4	48	48	CD
cord-014597-66vd2mdu	1699	5	h	h	NN
cord-014597-66vd2mdu	1699	6	after	after	IN
cord-014597-66vd2mdu	1699	7	transfection	transfection	NN
cord-014597-66vd2mdu	1699	8	using	use	VBG
cord-014597-66vd2mdu	1699	9	protein	protein	NN
cord-014597-66vd2mdu	1699	10	G	g	NN
cord-014597-66vd2mdu	1699	11	affinity	affinity	NN
cord-014597-66vd2mdu	1699	12	quantification	quantification	NN
cord-014597-66vd2mdu	1699	13	(	(	-LRB-
cord-014597-66vd2mdu	1699	14	HPLC	HPLC	NNP
cord-014597-66vd2mdu	1699	15	)	)	-RRB-
cord-014597-66vd2mdu	1699	16	Monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1699	17	antibodies	antibody	NNS
cord-014597-66vd2mdu	1699	18	(	(	-LRB-
cord-014597-66vd2mdu	1699	19	MAbs	MAbs	NNP
cord-014597-66vd2mdu	1699	20	)	)	-RRB-
cord-014597-66vd2mdu	1699	21	,	,	,
cord-014597-66vd2mdu	1699	22	which	which	WDT
cord-014597-66vd2mdu	1699	23	are	be	VBP
cord-014597-66vd2mdu	1699	24	widely	widely	RB
cord-014597-66vd2mdu	1699	25	used	use	VBN
cord-014597-66vd2mdu	1699	26	in	in	IN
cord-014597-66vd2mdu	1699	27	anticancer	anticancer	JJ
cord-014597-66vd2mdu	1699	28	therapies	therapy	NNS
cord-014597-66vd2mdu	1699	29	,	,	,
cord-014597-66vd2mdu	1699	30	are	be	VBP
cord-014597-66vd2mdu	1699	31	mainly	mainly	RB
cord-014597-66vd2mdu	1699	32	produced	produce	VBN
cord-014597-66vd2mdu	1699	33	by	by	IN
cord-014597-66vd2mdu	1699	34	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1699	35	cell	cell	NN
cord-014597-66vd2mdu	1699	36	lines	line	NNS
cord-014597-66vd2mdu	1699	37	.	.	.
cord-014597-66vd2mdu	1700	1	MAb	MAb	NNS
cord-014597-66vd2mdu	1700	2	conjugation	conjugation	NN
cord-014597-66vd2mdu	1700	3	to	to	IN
cord-014597-66vd2mdu	1700	4	biological	biological	JJ
cord-014597-66vd2mdu	1700	5	molecules	molecule	NNS
cord-014597-66vd2mdu	1700	6	for	for	IN
cord-014597-66vd2mdu	1700	7	enhancing	enhance	VBG
cord-014597-66vd2mdu	1700	8	their	-PRON-	PRP$
cord-014597-66vd2mdu	1700	9	antitumor	antitumor	JJ
cord-014597-66vd2mdu	1700	10	activity	activity	NN
cord-014597-66vd2mdu	1700	11	offers	offer	VBZ
cord-014597-66vd2mdu	1700	12	a	a	DT
cord-014597-66vd2mdu	1700	13	new	new	JJ
cord-014597-66vd2mdu	1700	14	powerful	powerful	JJ
cord-014597-66vd2mdu	1700	15	tool	tool	NN
cord-014597-66vd2mdu	1700	16	for	for	IN
cord-014597-66vd2mdu	1700	17	anticancer	anticancer	JJ
cord-014597-66vd2mdu	1700	18	therapies	therapy	NNS
cord-014597-66vd2mdu	1700	19	.	.	.
cord-014597-66vd2mdu	1701	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1701	2	have	have	VBP
cord-014597-66vd2mdu	1701	3	assessed	assess	VBN
cord-014597-66vd2mdu	1701	4	the	the	DT
cord-014597-66vd2mdu	1701	5	production	production	NN
cord-014597-66vd2mdu	1701	6	of	of	IN
cord-014597-66vd2mdu	1701	7	commercially	commercially	RB
cord-014597-66vd2mdu	1701	8	approved	approve	VBN
cord-014597-66vd2mdu	1701	9	anti	anti	JJ
cord-014597-66vd2mdu	1701	10	-	-	JJ
cord-014597-66vd2mdu	1701	11	HER2	her2	JJ
cord-014597-66vd2mdu	1701	12	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1701	13	antibody	antibody	NN
cord-014597-66vd2mdu	1701	14	Trastuzumab	Trastuzumab	NNP
cord-014597-66vd2mdu	1701	15	(	(	-LRB-
cord-014597-66vd2mdu	1701	16	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1701	17	)	)	-RRB-
cord-014597-66vd2mdu	1701	18	[	[	-LRB-
cord-014597-66vd2mdu	1701	19	1	1	CD
cord-014597-66vd2mdu	1701	20	]	]	-RRB-
cord-014597-66vd2mdu	1701	21	and	and	CC
cord-014597-66vd2mdu	1701	22	also	also	RB
cord-014597-66vd2mdu	1701	23	its	-PRON-	PRP$
cord-014597-66vd2mdu	1701	24	fusion	fusion	NN
cord-014597-66vd2mdu	1701	25	with	with	IN
cord-014597-66vd2mdu	1701	26	interferon	interferon	NN
cord-014597-66vd2mdu	1701	27	-	-	HYPH
cord-014597-66vd2mdu	1701	28	α2b	α2b	JJ
cord-014597-66vd2mdu	1701	29	(	(	-LRB-
cord-014597-66vd2mdu	1701	30	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1701	31	)	)	-RRB-
cord-014597-66vd2mdu	1701	32	.	.	.
cord-014597-66vd2mdu	1702	1	Two	two	CD
cord-014597-66vd2mdu	1702	2	cloning	clone	VBG
cord-014597-66vd2mdu	1702	3	strategies	strategy	NNS
cord-014597-66vd2mdu	1702	4	consisting	consist	VBG
cord-014597-66vd2mdu	1702	5	in	in	IN
cord-014597-66vd2mdu	1702	6	transfecting	transfecte	VBG
cord-014597-66vd2mdu	1702	7	CHO	CHO	NNP
cord-014597-66vd2mdu	1702	8	-	-	HYPH
cord-014597-66vd2mdu	1702	9	S	S	NNP
cord-014597-66vd2mdu	1702	10	and	and	CC
cord-014597-66vd2mdu	1702	11	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1702	12	cell	cell	NN
cord-014597-66vd2mdu	1702	13	lines	line	NNS
cord-014597-66vd2mdu	1702	14	with	with	IN
cord-014597-66vd2mdu	1702	15	two	two	CD
cord-014597-66vd2mdu	1702	16	bicistronic	bicistronic	JJ
cord-014597-66vd2mdu	1702	17	or	or	CC
cord-014597-66vd2mdu	1702	18	with	with	IN
cord-014597-66vd2mdu	1702	19	a	a	DT
cord-014597-66vd2mdu	1702	20	single	single	JJ
cord-014597-66vd2mdu	1702	21	tricistronic	tricistronic	JJ
cord-014597-66vd2mdu	1702	22	plasmids	plasmid	NNS
cord-014597-66vd2mdu	1702	23	have	have	VBP
cord-014597-66vd2mdu	1702	24	been	be	VBN
cord-014597-66vd2mdu	1702	25	assessed	assess	VBN
cord-014597-66vd2mdu	1702	26	.	.	.
cord-014597-66vd2mdu	1703	1	The	the	DT
cord-014597-66vd2mdu	1703	2	in	in	FW
cord-014597-66vd2mdu	1703	3	vitro	vitro	FW
cord-014597-66vd2mdu	1703	4	efficacy	efficacy	NN
cord-014597-66vd2mdu	1703	5	of	of	IN
cord-014597-66vd2mdu	1703	6	both	both	DT
cord-014597-66vd2mdu	1703	7	antibodies	antibody	NNS
cord-014597-66vd2mdu	1703	8	has	have	VBZ
cord-014597-66vd2mdu	1703	9	been	be	VBN
cord-014597-66vd2mdu	1703	10	tested	test	VBN
cord-014597-66vd2mdu	1703	11	and	and	CC
cord-014597-66vd2mdu	1703	12	compared	compare	VBN
cord-014597-66vd2mdu	1703	13	side	side	NN
cord-014597-66vd2mdu	1703	14	by	by	IN
cord-014597-66vd2mdu	1703	15	side	side	NN
cord-014597-66vd2mdu	1703	16	.	.	.
cord-014597-66vd2mdu	1704	1	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1704	2	heavy	heavy	JJ
cord-014597-66vd2mdu	1704	3	and	and	CC
cord-014597-66vd2mdu	1704	4	light	light	JJ
cord-014597-66vd2mdu	1704	5	chains	chain	NNS
cord-014597-66vd2mdu	1704	6	were	be	VBD
cord-014597-66vd2mdu	1704	7	cloned	clone	VBN
cord-014597-66vd2mdu	1704	8	in	in	IN
cord-014597-66vd2mdu	1704	9	two	two	CD
cord-014597-66vd2mdu	1704	10	bicistronic	bicistronic	JJ
cord-014597-66vd2mdu	1704	11	plasmids	plasmid	NNS
cord-014597-66vd2mdu	1704	12	(	(	-LRB-
cord-014597-66vd2mdu	1704	13	pIRESpuro3	pIRESpuro3	NNPS
cord-014597-66vd2mdu	1704	14	and	and	CC
cord-014597-66vd2mdu	1704	15	pIRESneo3	pIRESneo3	NNP
cord-014597-66vd2mdu	1704	16	,	,	,
cord-014597-66vd2mdu	1704	17	Clontech	Clontech	NNP
cord-014597-66vd2mdu	1704	18	)	)	-RRB-
cord-014597-66vd2mdu	1704	19	and	and	CC
cord-014597-66vd2mdu	1704	20	in	in	IN
cord-014597-66vd2mdu	1704	21	a	a	DT
cord-014597-66vd2mdu	1704	22	tricistronic	tricistronic	JJ
cord-014597-66vd2mdu	1704	23	plasmid	plasmid	NN
cord-014597-66vd2mdu	1704	24	derived	derive	VBN
cord-014597-66vd2mdu	1704	25	from	from	IN
cord-014597-66vd2mdu	1704	26	pIRESpuro3	pIRESpuro3	NNP
cord-014597-66vd2mdu	1704	27	.	.	.
cord-014597-66vd2mdu	1705	1	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1705	2	was	be	VBD
cord-014597-66vd2mdu	1705	3	spliced	splice	VBN
cord-014597-66vd2mdu	1705	4	to	to	IN
cord-014597-66vd2mdu	1705	5	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1705	6	heavy	heavy	JJ
cord-014597-66vd2mdu	1705	7	chain	chain	NN
cord-014597-66vd2mdu	1705	8	by	by	IN
cord-014597-66vd2mdu	1705	9	overlap	overlap	NN
cord-014597-66vd2mdu	1705	10	extension	extension	NN
cord-014597-66vd2mdu	1705	11	PCR	PCR	NNP
cord-014597-66vd2mdu	1705	12	and	and	CC
cord-014597-66vd2mdu	1705	13	the	the	DT
cord-014597-66vd2mdu	1705	14	resulting	result	VBG
cord-014597-66vd2mdu	1705	15	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1705	16	-	-	HYPH
cord-014597-66vd2mdu	1705	17	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1705	18	fusion	fusion	NN
cord-014597-66vd2mdu	1705	19	protein	protein	NN
cord-014597-66vd2mdu	1705	20	was	be	VBD
cord-014597-66vd2mdu	1705	21	also	also	RB
cord-014597-66vd2mdu	1705	22	cloned	clone	VBN
cord-014597-66vd2mdu	1705	23	in	in	IN
cord-014597-66vd2mdu	1705	24	the	the	DT
cord-014597-66vd2mdu	1705	25	expression	expression	NN
cord-014597-66vd2mdu	1705	26	vectors	vector	NNS
cord-014597-66vd2mdu	1705	27	in	in	IN
cord-014597-66vd2mdu	1705	28	the	the	DT
cord-014597-66vd2mdu	1705	29	same	same	JJ
cord-014597-66vd2mdu	1705	30	way	way	NN
cord-014597-66vd2mdu	1705	31	than	than	IN
cord-014597-66vd2mdu	1705	32	non	non	JJ
cord-014597-66vd2mdu	1705	33	-	-	JJ
cord-014597-66vd2mdu	1705	34	modified	modified	JJ
cord-014597-66vd2mdu	1705	35	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1705	36	.	.	.
cord-014597-66vd2mdu	1706	1	Selected	select	VBN
cord-014597-66vd2mdu	1706	2	cell	cell	NN
cord-014597-66vd2mdu	1706	3	pools	pool	NNS
cord-014597-66vd2mdu	1706	4	were	be	VBD
cord-014597-66vd2mdu	1706	5	cultured	culture	VBN
cord-014597-66vd2mdu	1706	6	in	in	IN
cord-014597-66vd2mdu	1706	7	125	125	CD
cord-014597-66vd2mdu	1706	8	ml	ml	NN
cord-014597-66vd2mdu	1706	9	shake	shake	NN
cord-014597-66vd2mdu	1706	10	flasks	flask	NNS
cord-014597-66vd2mdu	1706	11	containing	contain	VBG
cord-014597-66vd2mdu	1706	12	SFMTransfx	sfmtransfx	NN
cord-014597-66vd2mdu	1706	13	supplemented	supplement	VBN
cord-014597-66vd2mdu	1706	14	with	with	IN
cord-014597-66vd2mdu	1706	15	10	10	CD
cord-014597-66vd2mdu	1706	16	%	%	NN
cord-014597-66vd2mdu	1706	17	v	v	NN
cord-014597-66vd2mdu	1706	18	/	/	SYM
cord-014597-66vd2mdu	1706	19	v	v	NN
cord-014597-66vd2mdu	1706	20	of	of	IN
cord-014597-66vd2mdu	1706	21	Cell	Cell	NNP
cord-014597-66vd2mdu	1706	22	Boost	Boost	NNP
cord-014597-66vd2mdu	1706	23	5	5	CD
cord-014597-66vd2mdu	1706	24	(	(	-LRB-
cord-014597-66vd2mdu	1706	25	HyClone	HyClone	NNP
cord-014597-66vd2mdu	1706	26	)	)	-RRB-
cord-014597-66vd2mdu	1706	27	,	,	,
cord-014597-66vd2mdu	1706	28	4	4	CD
cord-014597-66vd2mdu	1706	29	mM	mm	CD
cord-014597-66vd2mdu	1706	30	of	of	IN
cord-014597-66vd2mdu	1706	31	GlutaMAX	GlutaMAX	NNP
cord-014597-66vd2mdu	1707	1	(	(	-LRB-
cord-014597-66vd2mdu	1707	2	Gibco	Gibco	NNP
cord-014597-66vd2mdu	1707	3	)	)	-RRB-
cord-014597-66vd2mdu	1707	4	and	and	CC
cord-014597-66vd2mdu	1707	5	2	2	CD
cord-014597-66vd2mdu	1707	6	μg	μg	NN
cord-014597-66vd2mdu	1707	7	/	/	SYM
cord-014597-66vd2mdu	1707	8	ml	ml	NNS
cord-014597-66vd2mdu	1707	9	of	of	IN
cord-014597-66vd2mdu	1707	10	puromycin	puromycin	NNS
cord-014597-66vd2mdu	1707	11	and	and	CC
cord-014597-66vd2mdu	1707	12	also	also	RB
cord-014597-66vd2mdu	1707	13	with	with	IN
cord-014597-66vd2mdu	1707	14	700	700	CD
cord-014597-66vd2mdu	1707	15	μg	μg	NNP
cord-014597-66vd2mdu	1707	16	/	/	SYM
cord-014597-66vd2mdu	1707	17	ml	ml	CC
cord-014597-66vd2mdu	1707	18	neomycin	neomycin	NN
cord-014597-66vd2mdu	1707	19	in	in	IN
cord-014597-66vd2mdu	1707	20	the	the	DT
cord-014597-66vd2mdu	1707	21	case	case	NN
cord-014597-66vd2mdu	1707	22	of	of	IN
cord-014597-66vd2mdu	1707	23	the	the	DT
cord-014597-66vd2mdu	1707	24	cells	cell	NNS
cord-014597-66vd2mdu	1707	25	transfected	transfecte	VBN
cord-014597-66vd2mdu	1707	26	with	with	IN
cord-014597-66vd2mdu	1707	27	pIRES	pIRES	NNP
cord-014597-66vd2mdu	1707	28	-	-	HYPH
cord-014597-66vd2mdu	1707	29	neo3	neo3	NNP
cord-014597-66vd2mdu	1707	30	.	.	.
cord-014597-66vd2mdu	1708	1	Cells	cell	NNS
cord-014597-66vd2mdu	1708	2	were	be	VBD
cord-014597-66vd2mdu	1708	3	cultivated	cultivate	VBN
cord-014597-66vd2mdu	1708	4	in	in	IN
cord-014597-66vd2mdu	1708	5	the	the	DT
cord-014597-66vd2mdu	1708	6	same	same	JJ
cord-014597-66vd2mdu	1708	7	conditions	condition	NNS
cord-014597-66vd2mdu	1708	8	as	as	IN
cord-014597-66vd2mdu	1708	9	described	describe	VBN
cord-014597-66vd2mdu	1708	10	elsewhere	elsewhere	RB
cord-014597-66vd2mdu	1708	11	[	[	-LRB-
cord-014597-66vd2mdu	1708	12	2	2	CD
cord-014597-66vd2mdu	1708	13	]	]	-RRB-
cord-014597-66vd2mdu	1708	14	.	.	.
cord-014597-66vd2mdu	1709	1	Purified	purify	VBN
cord-014597-66vd2mdu	1709	2	products	product	NNS
cord-014597-66vd2mdu	1709	3	(	(	-LRB-
cord-014597-66vd2mdu	1709	4	using	use	VBG
cord-014597-66vd2mdu	1709	5	protein	protein	NN
cord-014597-66vd2mdu	1709	6	A	a	DT
cord-014597-66vd2mdu	1709	7	chromatography	chromatography	NN
cord-014597-66vd2mdu	1709	8	(	(	-LRB-
cord-014597-66vd2mdu	1709	9	HiTrap	HiTrap	NNP
cord-014597-66vd2mdu	1709	10	MabSelect	MabSelect	NNP
cord-014597-66vd2mdu	1710	1	Sure	sure	UH
cord-014597-66vd2mdu	1710	2	,	,	,
cord-014597-66vd2mdu	1710	3	ÄKTA	ÄKTA	NNP
cord-014597-66vd2mdu	1710	4	Avant	Avant	NNP
cord-014597-66vd2mdu	1710	5	150	150	CD
cord-014597-66vd2mdu	1710	6	)	)	-RRB-
cord-014597-66vd2mdu	1710	7	)	)	-RRB-
cord-014597-66vd2mdu	1710	8	were	be	VBD
cord-014597-66vd2mdu	1710	9	quantified	quantify	VBN
cord-014597-66vd2mdu	1710	10	by	by	IN
cord-014597-66vd2mdu	1710	11	both	both	CC
cord-014597-66vd2mdu	1710	12	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1710	13	and	and	CC
cord-014597-66vd2mdu	1710	14	SDS	SDS	NNP
cord-014597-66vd2mdu	1710	15	-	-	HYPH
cord-014597-66vd2mdu	1710	16	PAGE	PAGE	NNP
cord-014597-66vd2mdu	1710	17	.	.	.
cord-014597-66vd2mdu	1711	1	Antigen	antigen	NN
cord-014597-66vd2mdu	1711	2	binding	binding	JJ
cord-014597-66vd2mdu	1711	3	test	test	NN
cord-014597-66vd2mdu	1711	4	was	be	VBD
cord-014597-66vd2mdu	1711	5	performed	perform	VBN
cord-014597-66vd2mdu	1711	6	in	in	IN
cord-014597-66vd2mdu	1711	7	Sk	Sk	NNP
cord-014597-66vd2mdu	1711	8	-	-	HYPH
cord-014597-66vd2mdu	1711	9	br-3	br-3	NNP
cord-014597-66vd2mdu	1711	10	breast	breast	NN
cord-014597-66vd2mdu	1711	11	cancer	cancer	NN
cord-014597-66vd2mdu	1711	12	cell	cell	NN
cord-014597-66vd2mdu	1711	13	line	line	NN
cord-014597-66vd2mdu	1711	14	by	by	IN
cord-014597-66vd2mdu	1711	15	means	mean	NNS
cord-014597-66vd2mdu	1711	16	of	of	IN
cord-014597-66vd2mdu	1711	17	flow	flow	NN
cord-014597-66vd2mdu	1711	18	cytometry	cytometry	NN
cord-014597-66vd2mdu	1711	19	analysis	analysis	NN
cord-014597-66vd2mdu	1711	20	.	.	.
cord-014597-66vd2mdu	1712	1	The	the	DT
cord-014597-66vd2mdu	1712	2	biological	biological	JJ
cord-014597-66vd2mdu	1712	3	activity	activity	NN
cord-014597-66vd2mdu	1712	4	of	of	IN
cord-014597-66vd2mdu	1712	5	the	the	DT
cord-014597-66vd2mdu	1712	6	different	different	JJ
cord-014597-66vd2mdu	1712	7	candidates	candidate	NNS
cord-014597-66vd2mdu	1712	8	was	be	VBD
cord-014597-66vd2mdu	1712	9	tested	test	VBN
cord-014597-66vd2mdu	1712	10	with	with	IN
cord-014597-66vd2mdu	1712	11	MTT	MTT	NNP
cord-014597-66vd2mdu	1712	12	assay	assay	NN
cord-014597-66vd2mdu	1712	13	.	.	.
cord-014597-66vd2mdu	1713	1	Both	both	CC
cord-014597-66vd2mdu	1713	2	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1713	3	and	and	CC
cord-014597-66vd2mdu	1713	4	the	the	DT
cord-014597-66vd2mdu	1713	5	fusion	fusion	NN
cord-014597-66vd2mdu	1713	6	protein	protein	NN
cord-014597-66vd2mdu	1713	7	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1713	8	-	-	HYPH
cord-014597-66vd2mdu	1713	9	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1713	10	have	have	VBP
cord-014597-66vd2mdu	1713	11	been	be	VBN
cord-014597-66vd2mdu	1713	12	successfully	successfully	RB
cord-014597-66vd2mdu	1713	13	expressed	express	VBN
cord-014597-66vd2mdu	1713	14	in	in	IN
cord-014597-66vd2mdu	1713	15	CHO	CHO	NNP
cord-014597-66vd2mdu	1713	16	-	-	HYPH
cord-014597-66vd2mdu	1713	17	S	S	NNP
cord-014597-66vd2mdu	1713	18	and	and	CC
cord-014597-66vd2mdu	1713	19	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1713	20	,	,	,
cord-014597-66vd2mdu	1713	21	which	which	WDT
cord-014597-66vd2mdu	1713	22	use	use	VBP
cord-014597-66vd2mdu	1713	23	for	for	IN
cord-014597-66vd2mdu	1713	24	heterologous	heterologous	JJ
cord-014597-66vd2mdu	1713	25	protein	protein	NN
cord-014597-66vd2mdu	1713	26	expression	expression	NN
cord-014597-66vd2mdu	1713	27	have	have	VBP
cord-014597-66vd2mdu	1713	28	previously	previously	RB
cord-014597-66vd2mdu	1713	29	been	be	VBN
cord-014597-66vd2mdu	1713	30	optimized	optimize	VBN
cord-014597-66vd2mdu	1713	31	in	in	IN
cord-014597-66vd2mdu	1713	32	prior	prior	JJ
cord-014597-66vd2mdu	1713	33	works	work	NNS
cord-014597-66vd2mdu	1713	34	[	[	-LRB-
cord-014597-66vd2mdu	1713	35	3	3	CD
cord-014597-66vd2mdu	1713	36	]	]	-RRB-
cord-014597-66vd2mdu	1713	37	.	.	.
cord-014597-66vd2mdu	1714	1	The	the	DT
cord-014597-66vd2mdu	1714	2	tricistronic	tricistronic	JJ
cord-014597-66vd2mdu	1714	3	strategy	strategy	NN
cord-014597-66vd2mdu	1714	4	resulted	result	VBD
cord-014597-66vd2mdu	1714	5	in	in	IN
cord-014597-66vd2mdu	1714	6	the	the	DT
cord-014597-66vd2mdu	1714	7	most	most	RBS
cord-014597-66vd2mdu	1714	8	efficient	efficient	JJ
cord-014597-66vd2mdu	1714	9	,	,	,
cord-014597-66vd2mdu	1714	10	showing	show	VBG
cord-014597-66vd2mdu	1714	11	a	a	DT
cord-014597-66vd2mdu	1714	12	3.5fold	3.5fold	CD
cord-014597-66vd2mdu	1714	13	increase	increase	NN
cord-014597-66vd2mdu	1714	14	in	in	IN
cord-014597-66vd2mdu	1714	15	terms	term	NNS
cord-014597-66vd2mdu	1714	16	of	of	IN
cord-014597-66vd2mdu	1714	17	productivity	productivity	NN
cord-014597-66vd2mdu	1714	18	with	with	IN
cord-014597-66vd2mdu	1714	19	respect	respect	NN
cord-014597-66vd2mdu	1714	20	to	to	IN
cord-014597-66vd2mdu	1714	21	the	the	DT
cord-014597-66vd2mdu	1714	22	bicistronic	bicistronic	JJ
cord-014597-66vd2mdu	1714	23	double	double	JJ
cord-014597-66vd2mdu	1714	24	-	-	HYPH
cord-014597-66vd2mdu	1714	25	transfection	transfection	NN
cord-014597-66vd2mdu	1714	26	for	for	IN
cord-014597-66vd2mdu	1714	27	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1714	28	in	in	IN
cord-014597-66vd2mdu	1714	29	CHO	CHO	NNP
cord-014597-66vd2mdu	1714	30	-	-	HYPH
cord-014597-66vd2mdu	1714	31	S	S	NNP
cord-014597-66vd2mdu	1714	32	cells	cell	NNS
cord-014597-66vd2mdu	1714	33	and	and	CC
cord-014597-66vd2mdu	1714	34	a	a	DT
cord-014597-66vd2mdu	1714	35	5-fold	5-fold	CD
cord-014597-66vd2mdu	1714	36	increase	increase	NN
cord-014597-66vd2mdu	1714	37	in	in	IN
cord-014597-66vd2mdu	1714	38	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1714	39	cells	cell	NNS
cord-014597-66vd2mdu	1714	40	(	(	-LRB-
cord-014597-66vd2mdu	1714	41	Fig	fig	NN
cord-014597-66vd2mdu	1714	42	.	.	.
cord-014597-66vd2mdu	1714	43	1a	1a	CD
cord-014597-66vd2mdu	1714	44	)	)	-RRB-
cord-014597-66vd2mdu	1714	45	.	.	.
cord-014597-66vd2mdu	1715	1	In	in	IN
cord-014597-66vd2mdu	1715	2	the	the	DT
cord-014597-66vd2mdu	1715	3	case	case	NN
cord-014597-66vd2mdu	1715	4	of	of	IN
cord-014597-66vd2mdu	1715	5	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1715	6	-	-	HYPH
cord-014597-66vd2mdu	1715	7	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1715	8	,	,	,
cord-014597-66vd2mdu	1715	9	the	the	DT
cord-014597-66vd2mdu	1715	10	tricistronic	tricistronic	NNP
cord-014597-66vd2mdu	1715	11	strategy	strategy	NN
cord-014597-66vd2mdu	1715	12	also	also	RB
cord-014597-66vd2mdu	1715	13	allowed	allow	VBD
cord-014597-66vd2mdu	1715	14	to	to	TO
cord-014597-66vd2mdu	1715	15	achieve	achieve	VB
cord-014597-66vd2mdu	1715	16	higher	high	JJR
cord-014597-66vd2mdu	1715	17	productivities	productivity	NNS
cord-014597-66vd2mdu	1715	18	than	than	IN
cord-014597-66vd2mdu	1715	19	the	the	DT
cord-014597-66vd2mdu	1715	20	bicistronic	bicistronic	JJ
cord-014597-66vd2mdu	1715	21	one	one	CD
cord-014597-66vd2mdu	1715	22	(	(	-LRB-
cord-014597-66vd2mdu	1715	23	Fig	Fig	NNP
cord-014597-66vd2mdu	1715	24	.	.	.
cord-014597-66vd2mdu	1715	25	1b	1b	CD
cord-014597-66vd2mdu	1715	26	)	)	-RRB-
cord-014597-66vd2mdu	1715	27	.	.	.
cord-014597-66vd2mdu	1716	1	Regarding	regard	VBG
cord-014597-66vd2mdu	1716	2	the	the	DT
cord-014597-66vd2mdu	1716	3	differences	difference	NNS
cord-014597-66vd2mdu	1716	4	of	of	IN
cord-014597-66vd2mdu	1716	5	specific	specific	JJ
cord-014597-66vd2mdu	1716	6	productivity	productivity	NN
cord-014597-66vd2mdu	1716	7	between	between	IN
cord-014597-66vd2mdu	1716	8	both	both	DT
cord-014597-66vd2mdu	1716	9	cell	cell	NN
cord-014597-66vd2mdu	1716	10	lines	line	NNS
cord-014597-66vd2mdu	1716	11	tested	test	VBN
cord-014597-66vd2mdu	1716	12	,	,	,
cord-014597-66vd2mdu	1716	13	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1716	14	emerged	emerge	VBD
cord-014597-66vd2mdu	1716	15	as	as	IN
cord-014597-66vd2mdu	1716	16	the	the	DT
cord-014597-66vd2mdu	1716	17	best	good	JJS
cord-014597-66vd2mdu	1716	18	production	production	NN
cord-014597-66vd2mdu	1716	19	host	host	NN
cord-014597-66vd2mdu	1716	20	candidate	candidate	NN
cord-014597-66vd2mdu	1716	21	,	,	,
cord-014597-66vd2mdu	1716	22	for	for	IN
cord-014597-66vd2mdu	1716	23	the	the	DT
cord-014597-66vd2mdu	1716	24	two	two	CD
cord-014597-66vd2mdu	1716	25	tested	test	VBN
cord-014597-66vd2mdu	1716	26	strategies	strategy	NNS
cord-014597-66vd2mdu	1716	27	(	(	-LRB-
cord-014597-66vd2mdu	1716	28	tricistronic	tricistronic	NNP
cord-014597-66vd2mdu	1716	29	and	and	CC
cord-014597-66vd2mdu	1716	30	bicistronic	bicistronic	NNP
cord-014597-66vd2mdu	1716	31	)	)	-RRB-
cord-014597-66vd2mdu	1716	32	and	and	CC
cord-014597-66vd2mdu	1716	33	for	for	IN
cord-014597-66vd2mdu	1716	34	the	the	DT
cord-014597-66vd2mdu	1716	35	two	two	CD
cord-014597-66vd2mdu	1716	36	produced	produce	VBN
cord-014597-66vd2mdu	1716	37	proteins	protein	NNS
cord-014597-66vd2mdu	1716	38	,	,	,
cord-014597-66vd2mdu	1716	39	showing	show	VBG
cord-014597-66vd2mdu	1716	40	a	a	DT
cord-014597-66vd2mdu	1716	41	1.5-fold	1.5-fold	CD
cord-014597-66vd2mdu	1716	42	increase	increase	NN
cord-014597-66vd2mdu	1716	43	in	in	IN
cord-014597-66vd2mdu	1716	44	terms	term	NNS
cord-014597-66vd2mdu	1716	45	of	of	IN
cord-014597-66vd2mdu	1716	46	productivity	productivity	NN
cord-014597-66vd2mdu	1716	47	with	with	IN
cord-014597-66vd2mdu	1716	48	respect	respect	NN
cord-014597-66vd2mdu	1716	49	to	to	IN
cord-014597-66vd2mdu	1716	50	CHO	CHO	NNP
cord-014597-66vd2mdu	1716	51	-	-	HYPH
cord-014597-66vd2mdu	1716	52	S	S	NNP
cord-014597-66vd2mdu	1716	53	cells	cell	NNS
cord-014597-66vd2mdu	1716	54	for	for	IN
cord-014597-66vd2mdu	1716	55	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1716	56	using	use	VBG
cord-014597-66vd2mdu	1716	57	the	the	DT
cord-014597-66vd2mdu	1716	58	tricistronic	tricistronic	NNP
cord-014597-66vd2mdu	1716	59	strategy	strategy	NN
cord-014597-66vd2mdu	1716	60	.	.	.
cord-014597-66vd2mdu	1717	1	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1717	2	and	and	CC
cord-014597-66vd2mdu	1717	3	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1717	4	-	-	HYPH
cord-014597-66vd2mdu	1717	5	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1717	6	were	be	VBD
cord-014597-66vd2mdu	1717	7	analysed	analyse	VBN
cord-014597-66vd2mdu	1717	8	in	in	IN
cord-014597-66vd2mdu	1717	9	terms	term	NNS
cord-014597-66vd2mdu	1717	10	of	of	IN
cord-014597-66vd2mdu	1717	11	their	-PRON-	PRP$
cord-014597-66vd2mdu	1717	12	antigen	antigen	NN
cord-014597-66vd2mdu	1717	13	binding	bind	VBG
cord-014597-66vd2mdu	1717	14	capacity	capacity	NN
cord-014597-66vd2mdu	1717	15	,	,	,
cord-014597-66vd2mdu	1717	16	and	and	CC
cord-014597-66vd2mdu	1717	17	both	both	DT
cord-014597-66vd2mdu	1717	18	were	be	VBD
cord-014597-66vd2mdu	1717	19	find	find	JJ
cord-014597-66vd2mdu	1717	20	to	to	TO
cord-014597-66vd2mdu	1717	21	efficiently	efficiently	RB
cord-014597-66vd2mdu	1717	22	bind	bind	VB
cord-014597-66vd2mdu	1717	23	to	to	IN
cord-014597-66vd2mdu	1717	24	HER2	HER2	NNP
cord-014597-66vd2mdu	1717	25	+	+	CC
cord-014597-66vd2mdu	1718	1	Skbr-3	skbr-3	JJ
cord-014597-66vd2mdu	1718	2	cells	cell	NNS
cord-014597-66vd2mdu	1718	3	(	(	-LRB-
cord-014597-66vd2mdu	1718	4	Fig	Fig	NNP
cord-014597-66vd2mdu	1718	5	.	.	.
cord-014597-66vd2mdu	1718	6	1c	1c	CD
cord-014597-66vd2mdu	1718	7	)	)	-RRB-
cord-014597-66vd2mdu	1718	8	.	.	.
cord-014597-66vd2mdu	1719	1	Thus	thus	RB
cord-014597-66vd2mdu	1719	2	,	,	,
cord-014597-66vd2mdu	1719	3	the	the	DT
cord-014597-66vd2mdu	1719	4	antibody	antibody	NN
cord-014597-66vd2mdu	1719	5	affinity	affinity	NN
cord-014597-66vd2mdu	1719	6	to	to	IN
cord-014597-66vd2mdu	1719	7	HER2	HER2	NNP
cord-014597-66vd2mdu	1719	8	antigen	antigen	NN
cord-014597-66vd2mdu	1719	9	has	have	VBZ
cord-014597-66vd2mdu	1719	10	not	not	RB
cord-014597-66vd2mdu	1719	11	been	be	VBN
cord-014597-66vd2mdu	1719	12	affected	affect	VBN
cord-014597-66vd2mdu	1719	13	when	when	WRB
cord-014597-66vd2mdu	1719	14	fused	fuse	VBN
cord-014597-66vd2mdu	1719	15	to	to	IN
cord-014597-66vd2mdu	1719	16	INF	INF	NNP
cord-014597-66vd2mdu	1719	17	-	-	HYPH
cord-014597-66vd2mdu	1719	18	α2b	α2b	JJ
cord-014597-66vd2mdu	1719	19	.	.	.
cord-014597-66vd2mdu	1720	1	Finally	finally	RB
cord-014597-66vd2mdu	1720	2	,	,	,
cord-014597-66vd2mdu	1720	3	antiproliferative	antiproliferative	JJ
cord-014597-66vd2mdu	1720	4	activity	activity	NN
cord-014597-66vd2mdu	1720	5	of	of	IN
cord-014597-66vd2mdu	1720	6	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1720	7	and	and	CC
cord-014597-66vd2mdu	1720	8	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1720	9	-	-	HYPH
cord-014597-66vd2mdu	1720	10	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1720	11	were	be	VBD
cord-014597-66vd2mdu	1720	12	assessed	assess	VBN
cord-014597-66vd2mdu	1720	13	on	on	IN
cord-014597-66vd2mdu	1720	14	the	the	DT
cord-014597-66vd2mdu	1720	15	same	same	JJ
cord-014597-66vd2mdu	1720	16	Sk	Sk	NNP
cord-014597-66vd2mdu	1720	17	-	-	HYPH
cord-014597-66vd2mdu	1720	18	br-3	br-3	NNP
cord-014597-66vd2mdu	1720	19	cells	cell	NNS
cord-014597-66vd2mdu	1720	20	.	.	.
cord-014597-66vd2mdu	1721	1	At	at	IN
cord-014597-66vd2mdu	1721	2	a	a	DT
cord-014597-66vd2mdu	1721	3	concentration	concentration	NN
cord-014597-66vd2mdu	1721	4	of	of	IN
cord-014597-66vd2mdu	1721	5	500	500	CD
cord-014597-66vd2mdu	1721	6	nM	nm	CD
cord-014597-66vd2mdu	1721	7	of	of	IN
cord-014597-66vd2mdu	1721	8	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1721	9	,	,	,
cord-014597-66vd2mdu	1721	10	and	and	CC
cord-014597-66vd2mdu	1721	11	after	after	IN
cord-014597-66vd2mdu	1721	12	a	a	DT
cord-014597-66vd2mdu	1721	13	72-hour	72-hour	CD
cord-014597-66vd2mdu	1721	14	incubation	incubation	NN
cord-014597-66vd2mdu	1721	15	,	,	,
cord-014597-66vd2mdu	1721	16	Sk	Sk	NNP
cord-014597-66vd2mdu	1721	17	-	-	HYPH
cord-014597-66vd2mdu	1721	18	br-3	br-3	NNP
cord-014597-66vd2mdu	1721	19	cells	cell	NNS
cord-014597-66vd2mdu	1721	20	presented	present	VBD
cord-014597-66vd2mdu	1721	21	a	a	DT
cord-014597-66vd2mdu	1721	22	83	83	CD
cord-014597-66vd2mdu	1721	23	%	%	NN
cord-014597-66vd2mdu	1721	24	growth	growth	NN
cord-014597-66vd2mdu	1721	25	with	with	IN
cord-014597-66vd2mdu	1721	26	respect	respect	NN
cord-014597-66vd2mdu	1721	27	to	to	IN
cord-014597-66vd2mdu	1721	28	the	the	DT
cord-014597-66vd2mdu	1721	29	untreated	untreated	JJ
cord-014597-66vd2mdu	1721	30	control	control	NN
cord-014597-66vd2mdu	1721	31	.	.	.
cord-014597-66vd2mdu	1722	1	However	however	RB
cord-014597-66vd2mdu	1722	2	,	,	,
cord-014597-66vd2mdu	1722	3	no	no	DT
cord-014597-66vd2mdu	1722	4	antiproliferative	antiproliferative	JJ
cord-014597-66vd2mdu	1722	5	effect	effect	NN
cord-014597-66vd2mdu	1722	6	was	be	VBD
cord-014597-66vd2mdu	1722	7	observed	observe	VBN
cord-014597-66vd2mdu	1722	8	for	for	IN
cord-014597-66vd2mdu	1722	9	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1722	10	-	-	HYPH
cord-014597-66vd2mdu	1722	11	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1723	1	(	(	-LRB-
cord-014597-66vd2mdu	1723	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1723	3	.	.	.
cord-014597-66vd2mdu	1723	4	1d	1d	CD
cord-014597-66vd2mdu	1723	5	)	)	-RRB-
cord-014597-66vd2mdu	1723	6	.	.	.
cord-014597-66vd2mdu	1724	1	The	the	DT
cord-014597-66vd2mdu	1724	2	tricistronic	tricistronic	NNP
cord-014597-66vd2mdu	1724	3	strategy	strategy	NN
cord-014597-66vd2mdu	1724	4	provides	provide	VBZ
cord-014597-66vd2mdu	1724	5	higher	high	JJR
cord-014597-66vd2mdu	1724	6	productivity	productivity	NN
cord-014597-66vd2mdu	1724	7	yields	yield	NNS
cord-014597-66vd2mdu	1724	8	in	in	IN
cord-014597-66vd2mdu	1724	9	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1724	10	and	and	CC
cord-014597-66vd2mdu	1724	11	CHO	CHO	NNP
cord-014597-66vd2mdu	1724	12	-	-	HYPH
cord-014597-66vd2mdu	1724	13	S	S	NNP
cord-014597-66vd2mdu	1724	14	cell	cell	NN
cord-014597-66vd2mdu	1724	15	lines	line	NNS
cord-014597-66vd2mdu	1724	16	for	for	IN
cord-014597-66vd2mdu	1724	17	both	both	DT
cord-014597-66vd2mdu	1724	18	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1724	19	proteins	protein	NNS
cord-014597-66vd2mdu	1724	20	(	(	-LRB-
cord-014597-66vd2mdu	1724	21	Trastuzumab	Trastuzumab	NNPS
cord-014597-66vd2mdu	1724	22	and	and	CC
cord-014597-66vd2mdu	1724	23	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1724	24	-	-	HYPH
cord-014597-66vd2mdu	1724	25	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1724	26	)	)	-RRB-
cord-014597-66vd2mdu	1724	27	.	.	.
cord-014597-66vd2mdu	1725	1	Regarding	regard	VBG
cord-014597-66vd2mdu	1725	2	which	which	WDT
cord-014597-66vd2mdu	1725	3	cell	cell	NN
cord-014597-66vd2mdu	1725	4	line	line	NN
cord-014597-66vd2mdu	1725	5	is	be	VBZ
cord-014597-66vd2mdu	1725	6	the	the	DT
cord-014597-66vd2mdu	1725	7	best	good	JJS
cord-014597-66vd2mdu	1725	8	production	production	NN
cord-014597-66vd2mdu	1725	9	host	host	NN
cord-014597-66vd2mdu	1725	10	candidate	candidate	NN
cord-014597-66vd2mdu	1725	11	,	,	,
cord-014597-66vd2mdu	1725	12	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1725	13	achieved	achieve	VBD
cord-014597-66vd2mdu	1725	14	higher	high	JJR
cord-014597-66vd2mdu	1725	15	productivity	productivity	NN
cord-014597-66vd2mdu	1725	16	than	than	IN
cord-014597-66vd2mdu	1725	17	CHO	CHO	NNP
cord-014597-66vd2mdu	1725	18	-	-	HYPH
cord-014597-66vd2mdu	1725	19	S	S	NNP
cord-014597-66vd2mdu	1725	20	cells	cell	NNS
cord-014597-66vd2mdu	1725	21	for	for	IN
cord-014597-66vd2mdu	1725	22	the	the	DT
cord-014597-66vd2mdu	1725	23	two	two	CD
cord-014597-66vd2mdu	1725	24	proteins	protein	NNS
cord-014597-66vd2mdu	1725	25	tested	test	VBN
cord-014597-66vd2mdu	1725	26	.	.	.
cord-014597-66vd2mdu	1726	1	All	all	DT
cord-014597-66vd2mdu	1726	2	constructions	construction	NNS
cord-014597-66vd2mdu	1726	3	performed	perform	VBN
cord-014597-66vd2mdu	1726	4	preserved	preserve	VBD
cord-014597-66vd2mdu	1726	5	the	the	DT
cord-014597-66vd2mdu	1726	6	binding	bind	VBG
cord-014597-66vd2mdu	1726	7	affinity	affinity	NN
cord-014597-66vd2mdu	1726	8	to	to	IN
cord-014597-66vd2mdu	1726	9	its	-PRON-	PRP$
cord-014597-66vd2mdu	1726	10	antigen	antigen	NN
cord-014597-66vd2mdu	1726	11	,	,	,
cord-014597-66vd2mdu	1726	12	Trastuzumab	Trastuzumab	NNP
cord-014597-66vd2mdu	1726	13	and	and	CC
cord-014597-66vd2mdu	1726	14	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1726	15	-	-	HYPH
cord-014597-66vd2mdu	1726	16	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1726	17	bind	bind	VBP
cord-014597-66vd2mdu	1726	18	efficiently	efficiently	RB
cord-014597-66vd2mdu	1726	19	to	to	IN
cord-014597-66vd2mdu	1726	20	the	the	DT
cord-014597-66vd2mdu	1726	21	HER2	HER2	NNP
cord-014597-66vd2mdu	1726	22	antigen	antigen	NN
cord-014597-66vd2mdu	1726	23	present	present	NN
cord-014597-66vd2mdu	1726	24	in	in	IN
cord-014597-66vd2mdu	1726	25	Skbr-3	Skbr-3	NNP
cord-014597-66vd2mdu	1726	26	cells	cell	NNS
cord-014597-66vd2mdu	1726	27	.	.	.
cord-014597-66vd2mdu	1727	1	Finally	finally	RB
cord-014597-66vd2mdu	1727	2	,	,	,
cord-014597-66vd2mdu	1727	3	Tzmb	Tzmb	NNP
cord-014597-66vd2mdu	1727	4	-	-	HYPH
cord-014597-66vd2mdu	1727	5	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1727	6	does	do	VBZ
cord-014597-66vd2mdu	1727	7	not	not	RB
cord-014597-66vd2mdu	1727	8	present	present	VB
cord-014597-66vd2mdu	1727	9	an	an	DT
cord-014597-66vd2mdu	1727	10	improved	improve	VBN
cord-014597-66vd2mdu	1727	11	antiproliferative	antiproliferative	JJ
cord-014597-66vd2mdu	1727	12	effect	effect	NN
cord-014597-66vd2mdu	1727	13	with	with	IN
cord-014597-66vd2mdu	1727	14	respect	respect	NN
cord-014597-66vd2mdu	1727	15	to	to	IN
cord-014597-66vd2mdu	1727	16	Trastuzumab	Trastuzumab	NNP
cord-014597-66vd2mdu	1727	17	when	when	WRB
cord-014597-66vd2mdu	1727	18	compared	compare	VBN
cord-014597-66vd2mdu	1727	19	by	by	IN
cord-014597-66vd2mdu	1727	20	means	mean	NNS
cord-014597-66vd2mdu	1727	21	of	of	IN
cord-014597-66vd2mdu	1727	22	an	an	DT
cord-014597-66vd2mdu	1727	23	in	in	FW
cord-014597-66vd2mdu	1727	24	vitro	vitro	FW
cord-014597-66vd2mdu	1727	25	assay	assay	NN
cord-014597-66vd2mdu	1727	26	.	.	.
cord-014597-66vd2mdu	1728	1	The	the	DT
cord-014597-66vd2mdu	1728	2	genetic	genetic	JJ
cord-014597-66vd2mdu	1728	3	engineering	engineering	NN
cord-014597-66vd2mdu	1728	4	of	of	IN
cord-014597-66vd2mdu	1728	5	patient	patient	NN
cord-014597-66vd2mdu	1728	6	-	-	HYPH
cord-014597-66vd2mdu	1728	7	specific	specific	JJ
cord-014597-66vd2mdu	1728	8	T	t	NN
cord-014597-66vd2mdu	1728	9	cells	cell	NNS
cord-014597-66vd2mdu	1728	10	with	with	IN
cord-014597-66vd2mdu	1728	11	lentiviral	lentiviral	JJ
cord-014597-66vd2mdu	1728	12	vectors	vector	NNS
cord-014597-66vd2mdu	1728	13	(	(	-LRB-
cord-014597-66vd2mdu	1728	14	LVV	LVV	NNP
cord-014597-66vd2mdu	1728	15	)	)	-RRB-
cord-014597-66vd2mdu	1728	16	expressing	express	VBG
cord-014597-66vd2mdu	1728	17	chimeric	chimeric	JJ
cord-014597-66vd2mdu	1728	18	antigen	antigen	NN
cord-014597-66vd2mdu	1728	19	receptors	receptor	NNS
cord-014597-66vd2mdu	1728	20	(	(	-LRB-
cord-014597-66vd2mdu	1728	21	CAR	car	NN
cord-014597-66vd2mdu	1728	22	)	)	-RRB-
cord-014597-66vd2mdu	1728	23	for	for	IN
cord-014597-66vd2mdu	1728	24	late	late	JJ
cord-014597-66vd2mdu	1728	25	phase	phase	NN
cord-014597-66vd2mdu	1728	26	clinical	clinical	JJ
cord-014597-66vd2mdu	1728	27	trials	trial	NNS
cord-014597-66vd2mdu	1728	28	requires	require	VBZ
cord-014597-66vd2mdu	1728	29	the	the	DT
cord-014597-66vd2mdu	1728	30	large	large	JJ
cord-014597-66vd2mdu	1728	31	-	-	HYPH
cord-014597-66vd2mdu	1728	32	scale	scale	NN
cord-014597-66vd2mdu	1728	33	manufacture	manufacture	NN
cord-014597-66vd2mdu	1728	34	of	of	IN
cord-014597-66vd2mdu	1728	35	high	high	JJ
cord-014597-66vd2mdu	1728	36	-	-	HYPH
cord-014597-66vd2mdu	1728	37	titer	titer	NN
cord-014597-66vd2mdu	1728	38	vector	vector	NN
cord-014597-66vd2mdu	1728	39	stocks	stock	NNS
cord-014597-66vd2mdu	1728	40	.	.	.
cord-014597-66vd2mdu	1729	1	The	the	DT
cord-014597-66vd2mdu	1729	2	state	state	NN
cord-014597-66vd2mdu	1729	3	-	-	HYPH
cord-014597-66vd2mdu	1729	4	of	of	IN
cord-014597-66vd2mdu	1729	5	-	-	HYPH
cord-014597-66vd2mdu	1729	6	the	the	DT
cord-014597-66vd2mdu	1729	7	-	-	HYPH
cord-014597-66vd2mdu	1729	8	art	art	NN
cord-014597-66vd2mdu	1729	9	production	production	NN
cord-014597-66vd2mdu	1729	10	of	of	IN
cord-014597-66vd2mdu	1729	11	LVV	LVV	NNP
cord-014597-66vd2mdu	1729	12	is	be	VBZ
cord-014597-66vd2mdu	1729	13	based	base	VBN
cord-014597-66vd2mdu	1729	14	on	on	IN
cord-014597-66vd2mdu	1729	15	10-to	10-to	CD
cord-014597-66vd2mdu	1729	16	40layer	40layer	CD
cord-014597-66vd2mdu	1729	17	cell	cell	NN
cord-014597-66vd2mdu	1729	18	factories	factory	NNS
cord-014597-66vd2mdu	1729	19	transiently	transiently	RB
cord-014597-66vd2mdu	1729	20	transfected	transfecte	VBN
cord-014597-66vd2mdu	1729	21	in	in	IN
cord-014597-66vd2mdu	1729	22	the	the	DT
cord-014597-66vd2mdu	1729	23	presence	presence	NN
cord-014597-66vd2mdu	1729	24	of	of	IN
cord-014597-66vd2mdu	1729	25	serum	serum	NN
cord-014597-66vd2mdu	1729	26	.	.	.
cord-014597-66vd2mdu	1730	1	This	this	DT
cord-014597-66vd2mdu	1730	2	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1730	3	process	process	NN
cord-014597-66vd2mdu	1730	4	is	be	VBZ
cord-014597-66vd2mdu	1730	5	extremely	extremely	RB
cord-014597-66vd2mdu	1730	6	limited	limit	VBN
cord-014597-66vd2mdu	1730	7	by	by	IN
cord-014597-66vd2mdu	1730	8	its	-PRON-	PRP$
cord-014597-66vd2mdu	1730	9	labor	labor	NN
cord-014597-66vd2mdu	1730	10	intensity	intensity	NN
cord-014597-66vd2mdu	1730	11	,	,	,
cord-014597-66vd2mdu	1730	12	open	open	JJ
cord-014597-66vd2mdu	1730	13	-	-	HYPH
cord-014597-66vd2mdu	1730	14	system	system	NN
cord-014597-66vd2mdu	1730	15	handling	handling	NN
cord-014597-66vd2mdu	1730	16	operations	operation	NNS
cord-014597-66vd2mdu	1730	17	,	,	,
cord-014597-66vd2mdu	1730	18	its	-PRON-	PRP$
cord-014597-66vd2mdu	1730	19	requirements	requirement	NNS
cord-014597-66vd2mdu	1730	20	for	for	IN
cord-014597-66vd2mdu	1730	21	significant	significant	JJ
cord-014597-66vd2mdu	1730	22	incubator	incubator	NN
cord-014597-66vd2mdu	1730	23	space	space	NN
cord-014597-66vd2mdu	1730	24	plus	plus	CC
cord-014597-66vd2mdu	1730	25	costs	cost	NNS
cord-014597-66vd2mdu	1730	26	and	and	CC
cord-014597-66vd2mdu	1730	27	patience	patience	NN
cord-014597-66vd2mdu	1730	28	risk	risk	NN
cord-014597-66vd2mdu	1730	29	due	due	JJ
cord-014597-66vd2mdu	1730	30	to	to	IN
cord-014597-66vd2mdu	1730	31	presence	presence	NN
cord-014597-66vd2mdu	1730	32	of	of	IN
cord-014597-66vd2mdu	1730	33	serum	serum	NN
cord-014597-66vd2mdu	1730	34	.	.	.
cord-014597-66vd2mdu	1731	1	To	to	TO
cord-014597-66vd2mdu	1731	2	circumvent	circumvent	VB
cord-014597-66vd2mdu	1731	3	these	these	DT
cord-014597-66vd2mdu	1731	4	limitations	limitation	NNS
cord-014597-66vd2mdu	1731	5	,	,	,
cord-014597-66vd2mdu	1731	6	this	this	DT
cord-014597-66vd2mdu	1731	7	study	study	NN
cord-014597-66vd2mdu	1731	8	aims	aim	VBZ
cord-014597-66vd2mdu	1731	9	to	to	TO
cord-014597-66vd2mdu	1731	10	develop	develop	VB
cord-014597-66vd2mdu	1731	11	a	a	DT
cord-014597-66vd2mdu	1731	12	stable	stable	JJ
cord-014597-66vd2mdu	1731	13	and	and	CC
cord-014597-66vd2mdu	1731	14	serum	serum	NN
cord-014597-66vd2mdu	1731	15	-	-	HYPH
cord-014597-66vd2mdu	1731	16	free	free	JJ
cord-014597-66vd2mdu	1731	17	process	process	NN
cord-014597-66vd2mdu	1731	18	to	to	TO
cord-014597-66vd2mdu	1731	19	produce	produce	VB
cord-014597-66vd2mdu	1731	20	LVV	LVV	NNP
cord-014597-66vd2mdu	1731	21	with	with	IN
cord-014597-66vd2mdu	1731	22	PEI	PEI	NNP
cord-014597-66vd2mdu	1731	23	-	-	HYPH
cord-014597-66vd2mdu	1731	24	mediated	mediate	VBN
cord-014597-66vd2mdu	1731	25	transfection	transfection	NN
cord-014597-66vd2mdu	1731	26	.	.	.
cord-014597-66vd2mdu	1732	1	In	in	IN
cord-014597-66vd2mdu	1732	2	addition	addition	NN
cord-014597-66vd2mdu	1732	3	,	,	,
cord-014597-66vd2mdu	1732	4	this	this	DT
cord-014597-66vd2mdu	1732	5	study	study	NN
cord-014597-66vd2mdu	1732	6	also	also	RB
cord-014597-66vd2mdu	1732	7	focuses	focus	VBZ
cord-014597-66vd2mdu	1732	8	on	on	IN
cord-014597-66vd2mdu	1732	9	the	the	DT
cord-014597-66vd2mdu	1732	10	development	development	NN
cord-014597-66vd2mdu	1732	11	of	of	IN
cord-014597-66vd2mdu	1732	12	a	a	DT
cord-014597-66vd2mdu	1732	13	a	a	DT
cord-014597-66vd2mdu	1732	14	c	c	NN
cord-014597-66vd2mdu	1732	15	b	b	NN
cord-014597-66vd2mdu	1732	16	d	d	NNP
cord-014597-66vd2mdu	1732	17	Fig	Fig	NNP
cord-014597-66vd2mdu	1732	18	.	.	.
cord-014597-66vd2mdu	1733	1	1	1	LS
cord-014597-66vd2mdu	1733	2	(	(	-LRB-
cord-014597-66vd2mdu	1733	3	abstract	abstract	NNP
cord-014597-66vd2mdu	1733	4	P-276	P-276	NNP
cord-014597-66vd2mdu	1733	5	)	)	-RRB-
cord-014597-66vd2mdu	1733	6	.	.	.
cord-014597-66vd2mdu	1734	1	Expression	expression	NN
cord-014597-66vd2mdu	1734	2	of	of	IN
cord-014597-66vd2mdu	1734	3	Trastuzumab	Trastuzumab	NNPS
cord-014597-66vd2mdu	1734	4	(	(	-LRB-
cord-014597-66vd2mdu	1734	5	a	a	LS
cord-014597-66vd2mdu	1734	6	)	)	-RRB-
cord-014597-66vd2mdu	1734	7	and	and	CC
cord-014597-66vd2mdu	1735	1	Trastuzumab	Trastuzumab	NNP
cord-014597-66vd2mdu	1735	2	-	-	HYPH
cord-014597-66vd2mdu	1735	3	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1735	4	(	(	-LRB-
cord-014597-66vd2mdu	1735	5	b	b	NN
cord-014597-66vd2mdu	1735	6	)	)	-RRB-
cord-014597-66vd2mdu	1735	7	from	from	IN
cord-014597-66vd2mdu	1735	8	bicistronic	bicistronic	JJ
cord-014597-66vd2mdu	1735	9	strategy	strategy	NN
cord-014597-66vd2mdu	1735	10	(	(	-LRB-
cord-014597-66vd2mdu	1735	11	BC	BC	NNP
cord-014597-66vd2mdu	1735	12	)	)	-RRB-
cord-014597-66vd2mdu	1735	13	and	and	CC
cord-014597-66vd2mdu	1735	14	tricistronic	tricistronic	NNP
cord-014597-66vd2mdu	1735	15	strategy	strategy	NN
cord-014597-66vd2mdu	1735	16	(	(	-LRB-
cord-014597-66vd2mdu	1735	17	TRI	TRI	NNP
cord-014597-66vd2mdu	1735	18	)	)	-RRB-
cord-014597-66vd2mdu	1735	19	with	with	IN
cord-014597-66vd2mdu	1735	20	CHO	CHO	NNP
cord-014597-66vd2mdu	1735	21	-	-	HYPH
cord-014597-66vd2mdu	1735	22	S	S	NNP
cord-014597-66vd2mdu	1735	23	and	and	CC
cord-014597-66vd2mdu	1735	24	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1735	25	cells	cell	NNS
cord-014597-66vd2mdu	1735	26	.	.	.
cord-014597-66vd2mdu	1736	1	Relative	relative	JJ
cord-014597-66vd2mdu	1736	2	specific	specific	JJ
cord-014597-66vd2mdu	1736	3	productivity	productivity	NN
cord-014597-66vd2mdu	1736	4	units	unit	NNS
cord-014597-66vd2mdu	1736	5	are	be	VBP
cord-014597-66vd2mdu	1736	6	used	use	VBN
cord-014597-66vd2mdu	1736	7	for	for	IN
cord-014597-66vd2mdu	1736	8	comparing	compare	VBG
cord-014597-66vd2mdu	1736	9	the	the	DT
cord-014597-66vd2mdu	1736	10	different	different	JJ
cord-014597-66vd2mdu	1736	11	strategies	strategy	NNS
cord-014597-66vd2mdu	1736	12	.	.	.
cord-014597-66vd2mdu	1737	1	c	c	NNP
cord-014597-66vd2mdu	1737	2	Antigen	Antigen	NNP
cord-014597-66vd2mdu	1737	3	binding	bind	VBG
cord-014597-66vd2mdu	1737	4	analysis	analysis	NN
cord-014597-66vd2mdu	1737	5	of	of	IN
cord-014597-66vd2mdu	1737	6	Trastuzumab	Trastuzumab	NNPS
cord-014597-66vd2mdu	1737	7	and	and	CC
cord-014597-66vd2mdu	1737	8	Trastuzumab	Trastuzumab	NNPS
cord-014597-66vd2mdu	1737	9	-	-	HYPH
cord-014597-66vd2mdu	1737	10	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1737	11	.	.	.
cord-014597-66vd2mdu	1738	1	d	d	NNP
cord-014597-66vd2mdu	1739	1	Antiproliferative	antiproliferative	JJ
cord-014597-66vd2mdu	1739	2	activity	activity	NN
cord-014597-66vd2mdu	1739	3	of	of	IN
cord-014597-66vd2mdu	1739	4	Trastuzumab	Trastuzumab	NNPS
cord-014597-66vd2mdu	1739	5	and	and	CC
cord-014597-66vd2mdu	1739	6	Trastuzumab	Trastuzumab	NNPS
cord-014597-66vd2mdu	1739	7	-	-	HYPH
cord-014597-66vd2mdu	1739	8	IFNα2b	IFNα2b	NNP
cord-014597-66vd2mdu	1739	9	on	on	IN
cord-014597-66vd2mdu	1739	10	Sk	Sk	NNP
cord-014597-66vd2mdu	1739	11	-	-	HYPH
cord-014597-66vd2mdu	1739	12	br-3	br-3	NNP
cord-014597-66vd2mdu	1739	13	cells	cell	NNS
cord-014597-66vd2mdu	1739	14	production	production	NN
cord-014597-66vd2mdu	1739	15	system	system	NN
cord-014597-66vd2mdu	1739	16	not	not	RB
cord-014597-66vd2mdu	1739	17	only	only	RB
cord-014597-66vd2mdu	1739	18	using	use	VBG
cord-014597-66vd2mdu	1739	19	a	a	DT
cord-014597-66vd2mdu	1739	20	GFP	GFP	NNP
cord-014597-66vd2mdu	1739	21	marker	marker	NN
cord-014597-66vd2mdu	1739	22	but	but	CC
cord-014597-66vd2mdu	1739	23	also	also	RB
cord-014597-66vd2mdu	1739	24	a	a	DT
cord-014597-66vd2mdu	1739	25	therapeutically	therapeutically	RB
cord-014597-66vd2mdu	1739	26	relevant	relevant	JJ
cord-014597-66vd2mdu	1739	27	transgene	transgene	NN
cord-014597-66vd2mdu	1739	28	(	(	-LRB-
cord-014597-66vd2mdu	1739	29	CD20-CAR	CD20-CAR	NNP
cord-014597-66vd2mdu	1739	30	)	)	-RRB-
cord-014597-66vd2mdu	1739	31	[	[	-LRB-
cord-014597-66vd2mdu	1739	32	1	1	CD
cord-014597-66vd2mdu	1739	33	]	]	-RRB-
cord-014597-66vd2mdu	1739	34	.	.	.
cord-014597-66vd2mdu	1740	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1740	2	,	,	,
cord-014597-66vd2mdu	1740	3	three	three	CD
cord-014597-66vd2mdu	1740	4	different	different	JJ
cord-014597-66vd2mdu	1740	5	cell	cell	NN
cord-014597-66vd2mdu	1740	6	lines	line	NNS
cord-014597-66vd2mdu	1740	7	(	(	-LRB-
cord-014597-66vd2mdu	1740	8	HEK	HEK	NNP
cord-014597-66vd2mdu	1740	9	293	293	CD
cord-014597-66vd2mdu	1740	10	,	,	,
cord-014597-66vd2mdu	1740	11	293	293	CD
cord-014597-66vd2mdu	1740	12	T	t	NN
cord-014597-66vd2mdu	1740	13	,	,	,
cord-014597-66vd2mdu	1740	14	293FT	293FT	NNP
cord-014597-66vd2mdu	1740	15	)	)	-RRB-
cord-014597-66vd2mdu	1740	16	were	be	VBD
cord-014597-66vd2mdu	1740	17	investigated	investigate	VBN
cord-014597-66vd2mdu	1740	18	concerning	concern	VBG
cord-014597-66vd2mdu	1740	19	their	-PRON-	PRP$
cord-014597-66vd2mdu	1740	20	productivity	productivity	NN
cord-014597-66vd2mdu	1740	21	of	of	IN
cord-014597-66vd2mdu	1740	22	LVV	LVV	NNP
cord-014597-66vd2mdu	1740	23	and	and	CC
cord-014597-66vd2mdu	1740	24	their	-PRON-	PRP$
cord-014597-66vd2mdu	1740	25	growing	grow	VBG
cord-014597-66vd2mdu	1740	26	behavior	behavior	NN
cord-014597-66vd2mdu	1740	27	in	in	IN
cord-014597-66vd2mdu	1740	28	the	the	DT
cord-014597-66vd2mdu	1740	29	in	in	IN
cord-014597-66vd2mdu	1740	30	-	-	HYPH
cord-014597-66vd2mdu	1740	31	house	house	NN
cord-014597-66vd2mdu	1740	32	serum	serum	NN
cord-014597-66vd2mdu	1740	33	-	-	HYPH
cord-014597-66vd2mdu	1740	34	free	free	JJ
cord-014597-66vd2mdu	1740	35	medium	medium	JJ
cord-014597-66vd2mdu	1740	36	TransMACS	transmac	NNS
cord-014597-66vd2mdu	1740	37	.	.	.
cord-014597-66vd2mdu	1741	1	As	as	IN
cord-014597-66vd2mdu	1741	2	part	part	NN
cord-014597-66vd2mdu	1741	3	of	of	IN
cord-014597-66vd2mdu	1741	4	this	this	DT
cord-014597-66vd2mdu	1741	5	,	,	,
cord-014597-66vd2mdu	1741	6	Design	Design	NNP
cord-014597-66vd2mdu	1741	7	of	of	IN
cord-014597-66vd2mdu	1741	8	Experiment	Experiment	NNP
cord-014597-66vd2mdu	1741	9	was	be	VBD
cord-014597-66vd2mdu	1741	10	used	use	VBN
cord-014597-66vd2mdu	1741	11	to	to	TO
cord-014597-66vd2mdu	1741	12	investigate	investigate	VB
cord-014597-66vd2mdu	1741	13	the	the	DT
cord-014597-66vd2mdu	1741	14	optimal	optimal	JJ
cord-014597-66vd2mdu	1741	15	conditions	condition	NNS
cord-014597-66vd2mdu	1741	16	for	for	IN
cord-014597-66vd2mdu	1741	17	PEI/	pei/	JJ
cord-014597-66vd2mdu	1741	18	DNA	dna	NN
cord-014597-66vd2mdu	1741	19	-	-	HYPH
cord-014597-66vd2mdu	1741	20	transfection	transfection	NN
cord-014597-66vd2mdu	1741	21	.	.	.
cord-014597-66vd2mdu	1742	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1742	2	,	,	,
cord-014597-66vd2mdu	1742	3	this	this	DT
cord-014597-66vd2mdu	1742	4	statistical	statistical	JJ
cord-014597-66vd2mdu	1742	5	approach	approach	NN
cord-014597-66vd2mdu	1742	6	was	be	VBD
cord-014597-66vd2mdu	1742	7	used	use	VBN
cord-014597-66vd2mdu	1742	8	focusing	focus	VBG
cord-014597-66vd2mdu	1742	9	an	an	DT
cord-014597-66vd2mdu	1742	10	ideal	ideal	JJ
cord-014597-66vd2mdu	1742	11	ratio	ratio	NN
cord-014597-66vd2mdu	1742	12	between	between	IN
cord-014597-66vd2mdu	1742	13	the	the	DT
cord-014597-66vd2mdu	1742	14	3rd	3rd	JJ
cord-014597-66vd2mdu	1742	15	generation	generation	NN
cord-014597-66vd2mdu	1742	16	plasmids	plasmid	NNS
cord-014597-66vd2mdu	1742	17	(	(	-LRB-
cord-014597-66vd2mdu	1742	18	transfer	transfer	NN
cord-014597-66vd2mdu	1742	19	plasmid	plasmid	NN
cord-014597-66vd2mdu	1742	20	CD20-CAR	CD20-CAR	NNP
cord-014597-66vd2mdu	1742	21	or	or	CC
cord-014597-66vd2mdu	1742	22	GFP	GFP	NNP
cord-014597-66vd2mdu	1742	23	,	,	,
cord-014597-66vd2mdu	1742	24	envelope	envelope	NN
cord-014597-66vd2mdu	1742	25	plasmid	plasmid	NN
cord-014597-66vd2mdu	1742	26	,	,	,
cord-014597-66vd2mdu	1742	27	packaging	packaging	NN
cord-014597-66vd2mdu	1742	28	plasmids	plasmid	NNS
cord-014597-66vd2mdu	1742	29	)	)	-RRB-
cord-014597-66vd2mdu	1742	30	.	.	.
cord-014597-66vd2mdu	1743	1	In	in	IN
cord-014597-66vd2mdu	1743	2	addition	addition	NN
cord-014597-66vd2mdu	1743	3	,	,	,
cord-014597-66vd2mdu	1743	4	different	different	JJ
cord-014597-66vd2mdu	1743	5	enhancers	enhancer	NNS
cord-014597-66vd2mdu	1743	6	(	(	-LRB-
cord-014597-66vd2mdu	1743	7	sodium	sodium	NN
cord-014597-66vd2mdu	1743	8	butyrate	butyrate	NN
cord-014597-66vd2mdu	1743	9	,	,	,
cord-014597-66vd2mdu	1743	10	lithium	lithium	NN
cord-014597-66vd2mdu	1743	11	acetate	acetate	NN
cord-014597-66vd2mdu	1743	12	,	,	,
cord-014597-66vd2mdu	1743	13	caffeine	caffeine	NN
cord-014597-66vd2mdu	1743	14	,	,	,
cord-014597-66vd2mdu	1743	15	trichostatin	trichostatin	NNP
cord-014597-66vd2mdu	1743	16	A	A	NNP
cord-014597-66vd2mdu	1743	17	,	,	,
cord-014597-66vd2mdu	1743	18	cholesterol	cholesterol	NN
cord-014597-66vd2mdu	1743	19	,	,	,
cord-014597-66vd2mdu	1743	20	hydroxyurea	hydroxyurea	NNP
cord-014597-66vd2mdu	1743	21	,	,	,
cord-014597-66vd2mdu	1743	22	valproic	valproic	NN
cord-014597-66vd2mdu	1743	23	acid	acid	NN
cord-014597-66vd2mdu	1743	24	)	)	-RRB-
cord-014597-66vd2mdu	1743	25	were	be	VBD
cord-014597-66vd2mdu	1743	26	investigated	investigate	VBN
cord-014597-66vd2mdu	1743	27	concerning	concern	VBG
cord-014597-66vd2mdu	1743	28	their	-PRON-	PRP$
cord-014597-66vd2mdu	1743	29	effects	effect	NNS
cord-014597-66vd2mdu	1743	30	on	on	IN
cord-014597-66vd2mdu	1743	31	productivity	productivity	NN
cord-014597-66vd2mdu	1743	32	comparing	compare	VBG
cord-014597-66vd2mdu	1743	33	HEK	HEK	NNP
cord-014597-66vd2mdu	1743	34	cultures	culture	NNS
cord-014597-66vd2mdu	1743	35	producing	produce	VBG
cord-014597-66vd2mdu	1743	36	LVV	lvv	NN
cord-014597-66vd2mdu	1743	37	encoding	encode	VBG
cord-014597-66vd2mdu	1743	38	for	for	IN
cord-014597-66vd2mdu	1743	39	GFP	gfp	NN
cord-014597-66vd2mdu	1743	40	-	-	HYPH
cord-014597-66vd2mdu	1743	41	marker	marker	NN
cord-014597-66vd2mdu	1743	42	or	or	CC
cord-014597-66vd2mdu	1743	43	CD20-CAR	cd20-car	NN
cord-014597-66vd2mdu	1743	44	.	.	.
cord-014597-66vd2mdu	1744	1	Concerning	concern	VBG
cord-014597-66vd2mdu	1744	2	productivity	productivity	NN
cord-014597-66vd2mdu	1744	3	and	and	CC
cord-014597-66vd2mdu	1744	4	growing	grow	VBG
cord-014597-66vd2mdu	1744	5	behavior	behavior	NN
cord-014597-66vd2mdu	1744	6	,	,	,
cord-014597-66vd2mdu	1744	7	HEK	HEK	NNP
cord-014597-66vd2mdu	1744	8	293	293	CD
cord-014597-66vd2mdu	1744	9	T	T	NNP
cord-014597-66vd2mdu	1744	10	was	be	VBD
cord-014597-66vd2mdu	1744	11	the	the	DT
cord-014597-66vd2mdu	1744	12	favored	favored	JJ
cord-014597-66vd2mdu	1744	13	cell	cell	NN
cord-014597-66vd2mdu	1744	14	line	line	NN
cord-014597-66vd2mdu	1744	15	for	for	IN
cord-014597-66vd2mdu	1744	16	our	-PRON-	PRP$
cord-014597-66vd2mdu	1744	17	serum	serum	NN
cord-014597-66vd2mdu	1744	18	-	-	HYPH
cord-014597-66vd2mdu	1744	19	free	free	JJ
cord-014597-66vd2mdu	1744	20	LV	LV	NNP
cord-014597-66vd2mdu	1744	21	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	1744	22	process	process	NN
cord-014597-66vd2mdu	1744	23	.	.	.
cord-014597-66vd2mdu	1745	1	In	in	IN
cord-014597-66vd2mdu	1745	2	addition	addition	NN
cord-014597-66vd2mdu	1745	3	,	,	,
cord-014597-66vd2mdu	1745	4	an	an	DT
cord-014597-66vd2mdu	1745	5	additive	additive	NN
cord-014597-66vd2mdu	1745	6	screen	screen	NN
cord-014597-66vd2mdu	1745	7	revealed	reveal	VBD
cord-014597-66vd2mdu	1745	8	that	that	IN
cord-014597-66vd2mdu	1745	9	sodium	sodium	NN
cord-014597-66vd2mdu	1745	10	butyrate	butyrate	NN
cord-014597-66vd2mdu	1745	11	alone	alone	RB
cord-014597-66vd2mdu	1745	12	had	have	VBD
cord-014597-66vd2mdu	1745	13	the	the	DT
cord-014597-66vd2mdu	1745	14	most	most	RBS
cord-014597-66vd2mdu	1745	15	promising	promising	JJ
cord-014597-66vd2mdu	1745	16	effect	effect	NN
cord-014597-66vd2mdu	1745	17	on	on	IN
cord-014597-66vd2mdu	1745	18	both	both	DT
cord-014597-66vd2mdu	1745	19	GFP	GFP	NNP
cord-014597-66vd2mdu	1745	20	-	-	HYPH
cord-014597-66vd2mdu	1745	21	LVV	LVV	NNP
cord-014597-66vd2mdu	1745	22	and	and	CC
cord-014597-66vd2mdu	1745	23	CD20-CAR	CD20-CAR	NNP
cord-014597-66vd2mdu	1745	24	-	-	HYPH
cord-014597-66vd2mdu	1745	25	LVV	LVV	NNP
cord-014597-66vd2mdu	1745	26	production	production	NN
cord-014597-66vd2mdu	1745	27	.	.	.
cord-014597-66vd2mdu	1746	1	After	after	IN
cord-014597-66vd2mdu	1746	2	PEI	PEI	NNP
cord-014597-66vd2mdu	1746	3	/	/	SYM
cord-014597-66vd2mdu	1746	4	DNA	dna	NN
cord-014597-66vd2mdu	1746	5	titration	titration	NN
cord-014597-66vd2mdu	1746	6	,	,	,
cord-014597-66vd2mdu	1746	7	we	-PRON-	PRP
cord-014597-66vd2mdu	1746	8	finally	finally	RB
cord-014597-66vd2mdu	1746	9	could	could	MD
cord-014597-66vd2mdu	1746	10	increase	increase	VB
cord-014597-66vd2mdu	1746	11	LVV	LVV	NNP
cord-014597-66vd2mdu	1746	12	productivity	productivity	NN
cord-014597-66vd2mdu	1746	13	by	by	IN
cord-014597-66vd2mdu	1746	14	lowering	lower	VBG
cord-014597-66vd2mdu	1746	15	PEI	PEI	NNP
cord-014597-66vd2mdu	1746	16	/	/	SYM
cord-014597-66vd2mdu	1746	17	DNA	dna	NN
cord-014597-66vd2mdu	1746	18	amount	amount	NN
cord-014597-66vd2mdu	1746	19	at	at	IN
cord-014597-66vd2mdu	1746	20	higher	high	JJR
cord-014597-66vd2mdu	1746	21	cell	cell	NN
cord-014597-66vd2mdu	1746	22	densities	density	NNS
cord-014597-66vd2mdu	1746	23	referred	refer	VBN
cord-014597-66vd2mdu	1746	24	to	to	IN
cord-014597-66vd2mdu	1746	25	our	-PRON-	PRP$
cord-014597-66vd2mdu	1746	26	standard	standard	JJ
cord-014597-66vd2mdu	1746	27	transfection	transfection	NN
cord-014597-66vd2mdu	1746	28	protocol	protocol	NN
cord-014597-66vd2mdu	1746	29	.	.	.
cord-014597-66vd2mdu	1747	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1747	2	,	,	,
cord-014597-66vd2mdu	1747	3	the	the	DT
cord-014597-66vd2mdu	1747	4	titration	titration	NN
cord-014597-66vd2mdu	1747	5	for	for	IN
cord-014597-66vd2mdu	1747	6	the	the	DT
cord-014597-66vd2mdu	1747	7	optimal	optimal	JJ
cord-014597-66vd2mdu	1747	8	plasmid	plasmid	NN
cord-014597-66vd2mdu	1747	9	ration	ration	NN
cord-014597-66vd2mdu	1747	10	revealed	reveal	VBN
cord-014597-66vd2mdu	1747	11	,	,	,
cord-014597-66vd2mdu	1747	12	that	that	IN
cord-014597-66vd2mdu	1747	13	for	for	IN
cord-014597-66vd2mdu	1747	14	large	large	JJ
cord-014597-66vd2mdu	1747	15	transfer	transfer	NN
cord-014597-66vd2mdu	1747	16	constructs	construct	VBZ
cord-014597-66vd2mdu	1747	17	higher	high	JJR
cord-014597-66vd2mdu	1747	18	amounts	amount	NNS
cord-014597-66vd2mdu	1747	19	of	of	IN
cord-014597-66vd2mdu	1747	20	transfer	transfer	NN
cord-014597-66vd2mdu	1747	21	plasmid	plasmid	NN
cord-014597-66vd2mdu	1747	22	are	be	VBP
cord-014597-66vd2mdu	1747	23	required	require	VBN
cord-014597-66vd2mdu	1747	24	than	than	IN
cord-014597-66vd2mdu	1747	25	for	for	IN
cord-014597-66vd2mdu	1747	26	smaller	small	JJR
cord-014597-66vd2mdu	1747	27	constructs	construct	NNS
cord-014597-66vd2mdu	1747	28	to	to	TO
cord-014597-66vd2mdu	1747	29	achieve	achieve	VB
cord-014597-66vd2mdu	1747	30	a	a	DT
cord-014597-66vd2mdu	1747	31	high	high	JJ
cord-014597-66vd2mdu	1747	32	productivity	productivity	NN
cord-014597-66vd2mdu	1747	33	(	(	-LRB-
cord-014597-66vd2mdu	1747	34	Fig	fig	NN
cord-014597-66vd2mdu	1747	35	.	.	.
cord-014597-66vd2mdu	1747	36	1	1	CD
cord-014597-66vd2mdu	1747	37	)	)	-RRB-
cord-014597-66vd2mdu	1747	38	.	.	.
cord-014597-66vd2mdu	1748	1	The	the	DT
cord-014597-66vd2mdu	1748	2	outcome	outcome	NN
cord-014597-66vd2mdu	1748	3	of	of	IN
cord-014597-66vd2mdu	1748	4	these	these	DT
cord-014597-66vd2mdu	1748	5	experiments	experiment	NNS
cord-014597-66vd2mdu	1748	6	enabled	enable	VBD
cord-014597-66vd2mdu	1748	7	the	the	DT
cord-014597-66vd2mdu	1748	8	development	development	NN
cord-014597-66vd2mdu	1748	9	of	of	IN
cord-014597-66vd2mdu	1748	10	a	a	DT
cord-014597-66vd2mdu	1748	11	robust	robust	JJ
cord-014597-66vd2mdu	1748	12	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1748	13	T	t	NN
cord-014597-66vd2mdu	1748	14	based	base	VBN
cord-014597-66vd2mdu	1748	15	process	process	NN
cord-014597-66vd2mdu	1748	16	to	to	TO
cord-014597-66vd2mdu	1748	17	produce	produce	VB
cord-014597-66vd2mdu	1748	18	clinical	clinical	JJ
cord-014597-66vd2mdu	1748	19	relevant	relevant	JJ
cord-014597-66vd2mdu	1748	20	LVV	LVV	NNP
cord-014597-66vd2mdu	1748	21	under	under	IN
cord-014597-66vd2mdu	1748	22	serumfree	serumfree	JJ
cord-014597-66vd2mdu	1748	23	conditions	condition	NNS
cord-014597-66vd2mdu	1748	24	.	.	.
cord-014597-66vd2mdu	1749	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1749	2	,	,	,
cord-014597-66vd2mdu	1749	3	it	-PRON-	PRP
cord-014597-66vd2mdu	1749	4	provides	provide	VBZ
cord-014597-66vd2mdu	1749	5	an	an	DT
cord-014597-66vd2mdu	1749	6	insight	insight	NN
cord-014597-66vd2mdu	1749	7	how	how	WRB
cord-014597-66vd2mdu	1749	8	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1749	9	genes	gene	NNS
cord-014597-66vd2mdu	1749	10	and	and	CC
cord-014597-66vd2mdu	1749	11	the	the	DT
cord-014597-66vd2mdu	1749	12	expression	expression	NN
cord-014597-66vd2mdu	1749	13	of	of	IN
cord-014597-66vd2mdu	1749	14	its	-PRON-	PRP$
cord-014597-66vd2mdu	1749	15	transgene	transgene	NN
cord-014597-66vd2mdu	1749	16	can	can	MD
cord-014597-66vd2mdu	1749	17	influence	influence	VB
cord-014597-66vd2mdu	1749	18	cell	cell	NN
cord-014597-66vd2mdu	1749	19	productivity	productivity	NN
cord-014597-66vd2mdu	1749	20	.	.	.
cord-014597-66vd2mdu	1750	1	led	lead	VBN
cord-014597-66vd2mdu	1750	2	to	to	IN
cord-014597-66vd2mdu	1750	3	a	a	DT
cord-014597-66vd2mdu	1750	4	vast	vast	JJ
cord-014597-66vd2mdu	1750	5	increase	increase	NN
cord-014597-66vd2mdu	1750	6	in	in	IN
cord-014597-66vd2mdu	1750	7	productivity	productivity	NN
cord-014597-66vd2mdu	1750	8	,	,	,
cord-014597-66vd2mdu	1750	9	CHO	CHO	NNP
cord-014597-66vd2mdu	1750	10	cells	cell	NNS
cord-014597-66vd2mdu	1750	11	yield	yield	VBP
cord-014597-66vd2mdu	1750	12	less	less	JJR
cord-014597-66vd2mdu	1750	13	than	than	IN
cord-014597-66vd2mdu	1750	14	other	other	JJ
cord-014597-66vd2mdu	1750	15	expression	expression	NN
cord-014597-66vd2mdu	1750	16	systems	system	NNS
cord-014597-66vd2mdu	1750	17	like	like	IN
cord-014597-66vd2mdu	1750	18	yeast	yeast	NN
cord-014597-66vd2mdu	1750	19	or	or	CC
cord-014597-66vd2mdu	1750	20	bacteria	bacteria	NNS
cord-014597-66vd2mdu	1750	21	[	[	-LRB-
cord-014597-66vd2mdu	1750	22	1	1	CD
cord-014597-66vd2mdu	1750	23	]	]	-RRB-
cord-014597-66vd2mdu	1750	24	.	.	.
cord-014597-66vd2mdu	1751	1	To	to	TO
cord-014597-66vd2mdu	1751	2	improve	improve	VB
cord-014597-66vd2mdu	1751	3	yields	yield	NNS
cord-014597-66vd2mdu	1751	4	and	and	CC
cord-014597-66vd2mdu	1751	5	find	find	VB
cord-014597-66vd2mdu	1751	6	beneficial	beneficial	JJ
cord-014597-66vd2mdu	1751	7	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	1751	8	phenotypes	phenotype	NNS
cord-014597-66vd2mdu	1751	9	,	,	,
cord-014597-66vd2mdu	1751	10	genetic	genetic	JJ
cord-014597-66vd2mdu	1751	11	engineering	engineering	NN
cord-014597-66vd2mdu	1751	12	plays	play	VBZ
cord-014597-66vd2mdu	1751	13	an	an	DT
cord-014597-66vd2mdu	1751	14	essential	essential	JJ
cord-014597-66vd2mdu	1751	15	role	role	NN
cord-014597-66vd2mdu	1751	16	in	in	IN
cord-014597-66vd2mdu	1751	17	recent	recent	JJ
cord-014597-66vd2mdu	1751	18	research	research	NN
cord-014597-66vd2mdu	1751	19	.	.	.
cord-014597-66vd2mdu	1752	1	The	the	DT
cord-014597-66vd2mdu	1752	2	miR-23	mir-23	NN
cord-014597-66vd2mdu	1752	3	cluster	cluster	NN
cord-014597-66vd2mdu	1752	4	with	with	IN
cord-014597-66vd2mdu	1752	5	its	-PRON-	PRP$
cord-014597-66vd2mdu	1752	6	genomic	genomic	JJ
cord-014597-66vd2mdu	1752	7	paralogues	paralogue	NNS
cord-014597-66vd2mdu	1752	8	(	(	-LRB-
cord-014597-66vd2mdu	1752	9	miR-23a	miR-23a	NNP
cord-014597-66vd2mdu	1752	10	and	and	CC
cord-014597-66vd2mdu	1752	11	miR-23b	miR-23b	NNP
cord-014597-66vd2mdu	1752	12	)	)	-RRB-
cord-014597-66vd2mdu	1752	13	was	be	VBD
cord-014597-66vd2mdu	1752	14	first	first	RB
cord-014597-66vd2mdu	1752	15	identified	identify	VBN
cord-014597-66vd2mdu	1752	16	as	as	IN
cord-014597-66vd2mdu	1752	17	differentially	differentially	RB
cord-014597-66vd2mdu	1752	18	expressed	express	VBN
cord-014597-66vd2mdu	1752	19	during	during	IN
cord-014597-66vd2mdu	1752	20	temperature	temperature	NN
cord-014597-66vd2mdu	1752	21	shift	shift	NN
cord-014597-66vd2mdu	1752	22	,	,	,
cord-014597-66vd2mdu	1752	23	suggesting	suggest	VBG
cord-014597-66vd2mdu	1752	24	its	-PRON-	PRP$
cord-014597-66vd2mdu	1752	25	role	role	NN
cord-014597-66vd2mdu	1752	26	in	in	IN
cord-014597-66vd2mdu	1752	27	proliferation	proliferation	NN
cord-014597-66vd2mdu	1752	28	and	and	CC
cord-014597-66vd2mdu	1752	29	productivity	productivity	NN
cord-014597-66vd2mdu	1752	30	[	[	-LRB-
cord-014597-66vd2mdu	1752	31	2	2	CD
cord-014597-66vd2mdu	1752	32	]	]	-RRB-
cord-014597-66vd2mdu	1752	33	.	.	.
cord-014597-66vd2mdu	1753	1	The	the	DT
cord-014597-66vd2mdu	1753	2	common	common	JJ
cord-014597-66vd2mdu	1753	3	approach	approach	NN
cord-014597-66vd2mdu	1753	4	to	to	TO
cord-014597-66vd2mdu	1753	5	deplete	deplete	VB
cord-014597-66vd2mdu	1753	6	miRNAs	miRNAs	NNP
cord-014597-66vd2mdu	1753	7	is	be	VBZ
cord-014597-66vd2mdu	1753	8	the	the	DT
cord-014597-66vd2mdu	1753	9	use	use	NN
cord-014597-66vd2mdu	1753	10	of	of	IN
cord-014597-66vd2mdu	1753	11	a	a	DT
cord-014597-66vd2mdu	1753	12	sponge	sponge	NN
cord-014597-66vd2mdu	1753	13	decoy	decoy	NN
cord-014597-66vd2mdu	1753	14	which	which	WDT
cord-014597-66vd2mdu	1753	15	,	,	,
cord-014597-66vd2mdu	1753	16	requires	require	VBZ
cord-014597-66vd2mdu	1753	17	the	the	DT
cord-014597-66vd2mdu	1753	18	introduction	introduction	NN
cord-014597-66vd2mdu	1753	19	of	of	IN
cord-014597-66vd2mdu	1753	20	reporter	reporter	NN
cord-014597-66vd2mdu	1753	21	genes	gene	NNS
cord-014597-66vd2mdu	1753	22	.	.	.
cord-014597-66vd2mdu	1754	1	As	as	IN
cord-014597-66vd2mdu	1754	2	an	an	DT
cord-014597-66vd2mdu	1754	3	alternative	alternative	NN
cord-014597-66vd2mdu	1754	4	this	this	DT
cord-014597-66vd2mdu	1754	5	work	work	NN
cord-014597-66vd2mdu	1754	6	aims	aim	VBZ
cord-014597-66vd2mdu	1754	7	to	to	TO
cord-014597-66vd2mdu	1754	8	knockdown	knockdown	VB
cord-014597-66vd2mdu	1754	9	miRNA	miRNA	NNP
cord-014597-66vd2mdu	1754	10	expression	expression	NN
cord-014597-66vd2mdu	1754	11	using	use	VBG
cord-014597-66vd2mdu	1754	12	the	the	DT
cord-014597-66vd2mdu	1754	13	recently	recently	RB
cord-014597-66vd2mdu	1754	14	developed	develop	VBN
cord-014597-66vd2mdu	1754	15	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	1754	16	/	/	SYM
cord-014597-66vd2mdu	1754	17	Cas9	Cas9	NNP
cord-014597-66vd2mdu	1754	18	system	system	NN
cord-014597-66vd2mdu	1754	19	which	which	WDT
cord-014597-66vd2mdu	1754	20	does	do	VBZ
cord-014597-66vd2mdu	1754	21	not	not	RB
cord-014597-66vd2mdu	1754	22	require	require	VB
cord-014597-66vd2mdu	1754	23	a	a	DT
cord-014597-66vd2mdu	1754	24	reporter	reporter	NN
cord-014597-66vd2mdu	1754	25	transcript	transcript	NN
cord-014597-66vd2mdu	1754	26	.	.	.
cord-014597-66vd2mdu	1755	1	This	this	DT
cord-014597-66vd2mdu	1755	2	system	system	NN
cord-014597-66vd2mdu	1755	3	consists	consist	VBZ
cord-014597-66vd2mdu	1755	4	of	of	IN
cord-014597-66vd2mdu	1755	5	two	two	CD
cord-014597-66vd2mdu	1755	6	main	main	JJ
cord-014597-66vd2mdu	1755	7	components	component	NNS
cord-014597-66vd2mdu	1755	8	:	:	:
cord-014597-66vd2mdu	1755	9	the	the	DT
cord-014597-66vd2mdu	1755	10	single	single	JJ
cord-014597-66vd2mdu	1755	11	guide	guide	NN
cord-014597-66vd2mdu	1755	12	RNA	RNA	NNP
cord-014597-66vd2mdu	1755	13	(	(	-LRB-
cord-014597-66vd2mdu	1755	14	sgRNA	sgRNA	VBG
cord-014597-66vd2mdu	1755	15	)	)	-RRB-
cord-014597-66vd2mdu	1755	16	and	and	CC
cord-014597-66vd2mdu	1755	17	an	an	DT
cord-014597-66vd2mdu	1755	18	endonuclease	endonuclease	NN
cord-014597-66vd2mdu	1755	19	(	(	-LRB-
cord-014597-66vd2mdu	1755	20	Cas9	Cas9	NNP
cord-014597-66vd2mdu	1755	21	)	)	-RRB-
cord-014597-66vd2mdu	1755	22	which	which	WDT
cord-014597-66vd2mdu	1755	23	induces	induce	VBZ
cord-014597-66vd2mdu	1755	24	double	double	JJ
cord-014597-66vd2mdu	1755	25	strand	strand	NN
cord-014597-66vd2mdu	1755	26	breaks	break	NNS
cord-014597-66vd2mdu	1755	27	(	(	-LRB-
cord-014597-66vd2mdu	1755	28	DSBs	dsb	NNS
cord-014597-66vd2mdu	1755	29	)	)	-RRB-
cord-014597-66vd2mdu	1755	30	.	.	.
cord-014597-66vd2mdu	1756	1	These	these	DT
cord-014597-66vd2mdu	1756	2	DSBs	dsb	NNS
cord-014597-66vd2mdu	1756	3	can	can	MD
cord-014597-66vd2mdu	1756	4	result	result	VB
cord-014597-66vd2mdu	1756	5	in	in	IN
cord-014597-66vd2mdu	1756	6	insertion	insertion	NN
cord-014597-66vd2mdu	1756	7	or	or	CC
cord-014597-66vd2mdu	1756	8	deletion	deletion	NN
cord-014597-66vd2mdu	1756	9	(	(	-LRB-
cord-014597-66vd2mdu	1756	10	indels	indel	NNS
cord-014597-66vd2mdu	1756	11	)	)	-RRB-
cord-014597-66vd2mdu	1756	12	of	of	IN
cord-014597-66vd2mdu	1756	13	base	base	NN
cord-014597-66vd2mdu	1756	14	pairs	pair	NNS
cord-014597-66vd2mdu	1756	15	which	which	WDT
cord-014597-66vd2mdu	1756	16	can	can	MD
cord-014597-66vd2mdu	1756	17	disrupt	disrupt	VB
cord-014597-66vd2mdu	1756	18	miRNA	miRNA	NNP
cord-014597-66vd2mdu	1756	19	function	function	NN
cord-014597-66vd2mdu	1756	20	and	and	CC
cord-014597-66vd2mdu	1756	21	processing	processing	NN
cord-014597-66vd2mdu	1756	22	[	[	-LRB-
cord-014597-66vd2mdu	1756	23	3	3	CD
cord-014597-66vd2mdu	1756	24	]	]	-RRB-
cord-014597-66vd2mdu	1756	25	.	.	.
cord-014597-66vd2mdu	1757	1	A	a	DT
cord-014597-66vd2mdu	1757	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1757	3	-	-	HYPH
cord-014597-66vd2mdu	1757	4	K1	K1	NNP
cord-014597-66vd2mdu	1757	5	cell	cell	NN
cord-014597-66vd2mdu	1757	6	line	line	NN
cord-014597-66vd2mdu	1757	7	stably	stably	RB
cord-014597-66vd2mdu	1757	8	expressing	express	VBG
cord-014597-66vd2mdu	1757	9	an	an	DT
cord-014597-66vd2mdu	1757	10	IgG	igg	NN
cord-014597-66vd2mdu	1757	11	was	be	VBD
cord-014597-66vd2mdu	1757	12	used	use	VBN
cord-014597-66vd2mdu	1757	13	for	for	IN
cord-014597-66vd2mdu	1757	14	knockdown	knockdown	NN
cord-014597-66vd2mdu	1757	15	experiments	experiment	NNS
cord-014597-66vd2mdu	1757	16	.	.	.
cord-014597-66vd2mdu	1758	1	SgRNAs	sgrna	NNS
cord-014597-66vd2mdu	1758	2	were	be	VBD
cord-014597-66vd2mdu	1758	3	designed	design	VBN
cord-014597-66vd2mdu	1758	4	to	to	TO
cord-014597-66vd2mdu	1758	5	target	target	VB
cord-014597-66vd2mdu	1758	6	the	the	DT
cord-014597-66vd2mdu	1758	7	seed	seed	NN
cord-014597-66vd2mdu	1758	8	region	region	NN
cord-014597-66vd2mdu	1758	9	of	of	IN
cord-014597-66vd2mdu	1758	10	each	each	DT
cord-014597-66vd2mdu	1758	11	miRNA	miRNA	NNP
cord-014597-66vd2mdu	1758	12	member	member	NN
cord-014597-66vd2mdu	1758	13	and	and	CC
cord-014597-66vd2mdu	1758	14	stable	stable	JJ
cord-014597-66vd2mdu	1758	15	mixed	mixed	JJ
cord-014597-66vd2mdu	1758	16	populations	population	NNS
cord-014597-66vd2mdu	1758	17	were	be	VBD
cord-014597-66vd2mdu	1758	18	generated	generate	VBN
cord-014597-66vd2mdu	1758	19	(	(	-LRB-
cord-014597-66vd2mdu	1758	20	Fig	Fig	NNP
cord-014597-66vd2mdu	1758	21	.	.	.
cord-014597-66vd2mdu	1758	22	1a	1a	CD
cord-014597-66vd2mdu	1758	23	)	)	-RRB-
cord-014597-66vd2mdu	1758	24	.	.	.
cord-014597-66vd2mdu	1759	1	Total	total	JJ
cord-014597-66vd2mdu	1759	2	RNA	RNA	NNP
cord-014597-66vd2mdu	1759	3	form	form	NN
cord-014597-66vd2mdu	1759	4	each	each	DT
cord-014597-66vd2mdu	1759	5	mixed	mixed	JJ
cord-014597-66vd2mdu	1759	6	population	population	NN
cord-014597-66vd2mdu	1759	7	was	be	VBD
cord-014597-66vd2mdu	1759	8	reverse	reverse	RB
cord-014597-66vd2mdu	1759	9	transcribed	transcribe	VBN
cord-014597-66vd2mdu	1759	10	into	into	IN
cord-014597-66vd2mdu	1759	11	cDNA	cdna	NN
cord-014597-66vd2mdu	1759	12	using	use	VBG
cord-014597-66vd2mdu	1759	13	miRNA	miRNA	NNP
cord-014597-66vd2mdu	1759	14	specific	specific	JJ
cord-014597-66vd2mdu	1759	15	stemloop	stemloop	JJ
cord-014597-66vd2mdu	1759	16	primers	primer	NNS
cord-014597-66vd2mdu	1759	17	.	.	.
cord-014597-66vd2mdu	1760	1	The	the	DT
cord-014597-66vd2mdu	1760	2	expression	expression	NN
cord-014597-66vd2mdu	1760	3	was	be	VBD
cord-014597-66vd2mdu	1760	4	quantified	quantify	VBN
cord-014597-66vd2mdu	1760	5	by	by	IN
cord-014597-66vd2mdu	1760	6	RT	RT	NNP
cord-014597-66vd2mdu	1760	7	-	-	HYPH
cord-014597-66vd2mdu	1760	8	qPCR	qPCR	NNP
cord-014597-66vd2mdu	1760	9	.	.	.
cord-014597-66vd2mdu	1761	1	To	to	TO
cord-014597-66vd2mdu	1761	2	further	further	RB
cord-014597-66vd2mdu	1761	3	analyse	analyse	VB
cord-014597-66vd2mdu	1761	4	the	the	DT
cord-014597-66vd2mdu	1761	5	range	range	NN
cord-014597-66vd2mdu	1761	6	of	of	IN
cord-014597-66vd2mdu	1761	7	indels	indel	NNS
cord-014597-66vd2mdu	1762	1	the	the	DT
cord-014597-66vd2mdu	1762	2	miR-23a	miR-23a	NNP
cord-014597-66vd2mdu	1762	3	and	and	CC
cord-014597-66vd2mdu	1762	4	miR-23b	miR-23b	NNP
cord-014597-66vd2mdu	1762	5	clusters	cluster	NNS
cord-014597-66vd2mdu	1762	6	were	be	VBD
cord-014597-66vd2mdu	1762	7	amplified	amplify	VBN
cord-014597-66vd2mdu	1762	8	by	by	IN
cord-014597-66vd2mdu	1762	9	a	a	DT
cord-014597-66vd2mdu	1762	10	standard	standard	JJ
cord-014597-66vd2mdu	1762	11	high	high	JJ
cord-014597-66vd2mdu	1762	12	-	-	HYPH
cord-014597-66vd2mdu	1762	13	fidelity	fidelity	NN
cord-014597-66vd2mdu	1762	14	PCR	PCR	NNP
cord-014597-66vd2mdu	1762	15	.	.	.
cord-014597-66vd2mdu	1763	1	Amplicons	amplicon	NNS
cord-014597-66vd2mdu	1763	2	were	be	VBD
cord-014597-66vd2mdu	1763	3	cloned	clone	VBN
cord-014597-66vd2mdu	1763	4	into	into	IN
cord-014597-66vd2mdu	1763	5	pCR	pCR	NNP
cord-014597-66vd2mdu	1763	6	TM	TM	NNP
cord-014597-66vd2mdu	1763	7	-TOPO	-TOPO	.
cord-014597-66vd2mdu	1764	1	®	®	LS
cord-014597-66vd2mdu	1764	2	vector	vector	NN
cord-014597-66vd2mdu	1764	3	and	and	CC
cord-014597-66vd2mdu	1764	4	positive	positive	JJ
cord-014597-66vd2mdu	1764	5	clones	clone	NNS
cord-014597-66vd2mdu	1764	6	were	be	VBD
cord-014597-66vd2mdu	1764	7	analysed	analyse	VBN
cord-014597-66vd2mdu	1764	8	by	by	IN
cord-014597-66vd2mdu	1764	9	Sanger	Sanger	NNP
cord-014597-66vd2mdu	1764	10	sequencing	sequencing	NN
cord-014597-66vd2mdu	1764	11	.	.	.
cord-014597-66vd2mdu	1765	1	Cell	cell	NN
cord-014597-66vd2mdu	1765	2	growth	growth	NN
cord-014597-66vd2mdu	1765	3	was	be	VBD
cord-014597-66vd2mdu	1765	4	monitored	monitor	VBN
cord-014597-66vd2mdu	1765	5	using	use	VBG
cord-014597-66vd2mdu	1765	6	ViaCount	ViaCount	NNP
cord-014597-66vd2mdu	1765	7	TM	TM	NNP
cord-014597-66vd2mdu	1765	8	viability	viability	NN
cord-014597-66vd2mdu	1765	9	stain	stain	NN
cord-014597-66vd2mdu	1765	10	on	on	IN
cord-014597-66vd2mdu	1765	11	a	a	DT
cord-014597-66vd2mdu	1765	12	Guava	Guava	NNP
cord-014597-66vd2mdu	1765	13	TM	TM	NNP
cord-014597-66vd2mdu	1765	14	benchtop	benchtop	NN
cord-014597-66vd2mdu	1765	15	flow	flow	NN
cord-014597-66vd2mdu	1765	16	cytometer	cytometer	NN
cord-014597-66vd2mdu	1765	17	.	.	.
cord-014597-66vd2mdu	1766	1	Productivity	productivity	NN
cord-014597-66vd2mdu	1766	2	was	be	VBD
cord-014597-66vd2mdu	1766	3	assessed	assess	VBN
cord-014597-66vd2mdu	1766	4	by	by	IN
cord-014597-66vd2mdu	1766	5	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1766	6	.	.	.
cord-014597-66vd2mdu	1767	1	Students	student	NNS
cord-014597-66vd2mdu	1767	2	t	t	NN
cord-014597-66vd2mdu	1767	3	-	-	HYPH
cord-014597-66vd2mdu	1767	4	test	test	NN
cord-014597-66vd2mdu	1767	5	was	be	VBD
cord-014597-66vd2mdu	1767	6	used	use	VBN
cord-014597-66vd2mdu	1767	7	for	for	IN
cord-014597-66vd2mdu	1767	8	statistical	statistical	JJ
cord-014597-66vd2mdu	1767	9	analysis	analysis	NN
cord-014597-66vd2mdu	1767	10	.	.	.
cord-014597-66vd2mdu	1768	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1768	2	was	be	VBD
cord-014597-66vd2mdu	1768	3	shown	show	VBN
cord-014597-66vd2mdu	1768	4	that	that	IN
cord-014597-66vd2mdu	1768	5	miRNA	miRNA	NNP
cord-014597-66vd2mdu	1768	6	expression	expression	NN
cord-014597-66vd2mdu	1768	7	was	be	VBD
cord-014597-66vd2mdu	1768	8	significantly	significantly	RB
cord-014597-66vd2mdu	1768	9	reduced	reduce	VBN
cord-014597-66vd2mdu	1768	10	in	in	IN
cord-014597-66vd2mdu	1768	11	mixed	mixed	JJ
cord-014597-66vd2mdu	1768	12	populations	population	NNS
cord-014597-66vd2mdu	1768	13	.	.	.
cord-014597-66vd2mdu	1769	1	A	a	DT
cord-014597-66vd2mdu	1769	2	knockdown	knockdown	NN
cord-014597-66vd2mdu	1769	3	up	up	IN
cord-014597-66vd2mdu	1769	4	to	to	TO
cord-014597-66vd2mdu	1769	5	95	95	CD
cord-014597-66vd2mdu	1769	6	%	%	NN
cord-014597-66vd2mdu	1769	7	was	be	VBD
cord-014597-66vd2mdu	1769	8	achieved	achieve	VBN
cord-014597-66vd2mdu	1769	9	for	for	IN
cord-014597-66vd2mdu	1769	10	miR-23a	miR-23a	NNP
cord-014597-66vd2mdu	1769	11	,	,	,
cord-014597-66vd2mdu	1769	12	miR-23b	miR-23b	NNP
cord-014597-66vd2mdu	1769	13	and	and	CC
cord-014597-66vd2mdu	1769	14	miR-24	miR-24	VBD
cord-014597-66vd2mdu	1769	15	.	.	.
cord-014597-66vd2mdu	1770	1	The	the	DT
cord-014597-66vd2mdu	1770	2	knockdown	knockdown	NN
cord-014597-66vd2mdu	1770	3	in	in	IN
cord-014597-66vd2mdu	1770	4	miR-27a	miR-27a	NNP
cord-014597-66vd2mdu	1770	5	and	and	CC
cord-014597-66vd2mdu	1770	6	miR-27b	miR-27b	NNP
cord-014597-66vd2mdu	1770	7	expression	expression	NN
cord-014597-66vd2mdu	1770	8	was	be	VBD
cord-014597-66vd2mdu	1770	9	considerably	considerably	RB
cord-014597-66vd2mdu	1770	10	less	less	JJR
cord-014597-66vd2mdu	1770	11	-between	-between	$
cord-014597-66vd2mdu	1770	12	70	70	CD
cord-014597-66vd2mdu	1770	13	-	-	SYM
cord-014597-66vd2mdu	1770	14	90	90	CD
cord-014597-66vd2mdu	1770	15	%	%	NN
cord-014597-66vd2mdu	1770	16	(	(	-LRB-
cord-014597-66vd2mdu	1770	17	n=3	n=3	NN
cord-014597-66vd2mdu	1770	18	,	,	,
cord-014597-66vd2mdu	1770	19	*	*	NFP
cord-014597-66vd2mdu	1770	20	P	p	NN
cord-014597-66vd2mdu	1770	21	≤	≤	NN
cord-014597-66vd2mdu	1770	22	0.05	0.05	CD
cord-014597-66vd2mdu	1770	23	,	,	,
cord-014597-66vd2mdu	1771	1	*	*	NFP
cord-014597-66vd2mdu	1771	2	*	*	NFP
cord-014597-66vd2mdu	1771	3	P	p	NN
cord-014597-66vd2mdu	1771	4	≤	≤	NN
cord-014597-66vd2mdu	1771	5	0.01	0.01	CD
cord-014597-66vd2mdu	1771	6	,	,	,
cord-014597-66vd2mdu	1772	1	*	*	NFP
cord-014597-66vd2mdu	1772	2	*	*	NFP
cord-014597-66vd2mdu	1772	3	*	*	NFP
cord-014597-66vd2mdu	1772	4	P	p	NN
cord-014597-66vd2mdu	1772	5	≤	≤	NN
cord-014597-66vd2mdu	1772	6	0.001	0.001	CD
cord-014597-66vd2mdu	1772	7	)	)	-RRB-
cord-014597-66vd2mdu	1773	1	(	(	-LRB-
cord-014597-66vd2mdu	1773	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1773	3	.	.	.
cord-014597-66vd2mdu	1773	4	1b	1b	CD
cord-014597-66vd2mdu	1773	5	)	)	-RRB-
cord-014597-66vd2mdu	1773	6	.	.	.
cord-014597-66vd2mdu	1774	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	1774	2	,	,	,
cord-014597-66vd2mdu	1774	3	it	-PRON-	PRP
cord-014597-66vd2mdu	1774	4	was	be	VBD
cord-014597-66vd2mdu	1774	5	shown	show	VBN
cord-014597-66vd2mdu	1774	6	that	that	IN
cord-014597-66vd2mdu	1774	7	various	various	JJ
cord-014597-66vd2mdu	1774	8	sizes	size	NNS
cord-014597-66vd2mdu	1774	9	of	of	IN
cord-014597-66vd2mdu	1774	10	indels	indel	NNS
cord-014597-66vd2mdu	1774	11	were	be	VBD
cord-014597-66vd2mdu	1774	12	generated	generate	VBN
cord-014597-66vd2mdu	1774	13	by	by	IN
cord-014597-66vd2mdu	1774	14	targeting	target	VBG
cord-014597-66vd2mdu	1774	15	the	the	DT
cord-014597-66vd2mdu	1774	16	seed	seed	NN
cord-014597-66vd2mdu	1774	17	region	region	NN
cord-014597-66vd2mdu	1774	18	.	.	.
cord-014597-66vd2mdu	1775	1	Smaller	small	JJR
cord-014597-66vd2mdu	1775	2	indels	indel	NNS
cord-014597-66vd2mdu	1775	3	(	(	-LRB-
cord-014597-66vd2mdu	1775	4	+1/	+1/	NNP
cord-014597-66vd2mdu	1775	5	+2/-1/-2	+2/-1/-2	NNP
cord-014597-66vd2mdu	1775	6	bps	bp	NNS
cord-014597-66vd2mdu	1775	7	)	)	-RRB-
cord-014597-66vd2mdu	1775	8	seemed	seem	VBD
cord-014597-66vd2mdu	1775	9	to	to	TO
cord-014597-66vd2mdu	1775	10	be	be	VB
cord-014597-66vd2mdu	1775	11	more	more	RBR
cord-014597-66vd2mdu	1775	12	common	common	JJ
cord-014597-66vd2mdu	1775	13	but	but	CC
cord-014597-66vd2mdu	1775	14	larger	large	JJR
cord-014597-66vd2mdu	1775	15	deletions	deletion	NNS
cord-014597-66vd2mdu	1775	16	were	be	VBD
cord-014597-66vd2mdu	1775	17	detected	detect	VBN
cord-014597-66vd2mdu	1775	18	as	as	RB
cord-014597-66vd2mdu	1775	19	well	well	RB
cord-014597-66vd2mdu	1776	1	(	(	-LRB-
cord-014597-66vd2mdu	1776	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1776	3	.	.	.
cord-014597-66vd2mdu	1776	4	1c	1c	CD
cord-014597-66vd2mdu	1776	5	)	)	-RRB-
cord-014597-66vd2mdu	1776	6	.	.	.
cord-014597-66vd2mdu	1777	1	MiR-23a	MiR-23a	NNP
cord-014597-66vd2mdu	1777	2	,	,	,
cord-014597-66vd2mdu	1777	3	miR-23b	miR-23b	NNP
cord-014597-66vd2mdu	1777	4	and	and	CC
cord-014597-66vd2mdu	1777	5	miR-27b	miR-27b	NNP
cord-014597-66vd2mdu	1777	6	showed	show	VBD
cord-014597-66vd2mdu	1777	7	increased	increased	JJ
cord-014597-66vd2mdu	1777	8	viability	viability	NN
cord-014597-66vd2mdu	1777	9	in	in	IN
cord-014597-66vd2mdu	1777	10	late	late	JJ
cord-014597-66vd2mdu	1777	11	stages	stage	NNS
cord-014597-66vd2mdu	1777	12	of	of	IN
cord-014597-66vd2mdu	1777	13	the	the	DT
cord-014597-66vd2mdu	1777	14	culture	culture	NN
cord-014597-66vd2mdu	1777	15	.	.	.
cord-014597-66vd2mdu	1778	1	Depletion	depletion	NN
cord-014597-66vd2mdu	1778	2	of	of	IN
cord-014597-66vd2mdu	1778	3	miR-27a	miR-27a	NNP
cord-014597-66vd2mdu	1778	4	reduced	reduce	VBN
cord-014597-66vd2mdu	1778	5	growth	growth	NN
cord-014597-66vd2mdu	1778	6	significantly	significantly	RB
cord-014597-66vd2mdu	1778	7	whereas	whereas	IN
cord-014597-66vd2mdu	1778	8	knockdown	knockdown	NN
cord-014597-66vd2mdu	1778	9	of	of	IN
cord-014597-66vd2mdu	1778	10	miR-24	mir-24	CC
cord-014597-66vd2mdu	1778	11	showed	show	VBD
cord-014597-66vd2mdu	1778	12	increased	increase	VBN
cord-014597-66vd2mdu	1778	13	proliferation	proliferation	NN
cord-014597-66vd2mdu	1778	14	as	as	RB
cord-014597-66vd2mdu	1778	15	well	well	RB
cord-014597-66vd2mdu	1778	16	as	as	IN
cord-014597-66vd2mdu	1778	17	boosting	boost	VBG
cord-014597-66vd2mdu	1778	18	IgG	igg	NN
cord-014597-66vd2mdu	1778	19	titers	titer	NNS
cord-014597-66vd2mdu	1778	20	(	(	-LRB-
cord-014597-66vd2mdu	1778	21	Table	Table	NNP
cord-014597-66vd2mdu	1778	22	1	1	CD
cord-014597-66vd2mdu	1778	23	)	)	-RRB-
cord-014597-66vd2mdu	1778	24	.	.	.
cord-014597-66vd2mdu	1779	1	In	in	IN
cord-014597-66vd2mdu	1779	2	this	this	DT
cord-014597-66vd2mdu	1779	3	work	work	NN
cord-014597-66vd2mdu	1779	4	,	,	,
cord-014597-66vd2mdu	1779	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1779	6	have	have	VBP
cord-014597-66vd2mdu	1779	7	shown	show	VBN
cord-014597-66vd2mdu	1779	8	that	that	IN
cord-014597-66vd2mdu	1779	9	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	1779	10	/	/	SYM
cord-014597-66vd2mdu	1779	11	Cas9	Cas9	NNP
cord-014597-66vd2mdu	1779	12	can	can	MD
cord-014597-66vd2mdu	1779	13	be	be	VB
cord-014597-66vd2mdu	1779	14	successfully	successfully	RB
cord-014597-66vd2mdu	1779	15	applied	apply	VBN
cord-014597-66vd2mdu	1779	16	as	as	IN
cord-014597-66vd2mdu	1779	17	a	a	DT
cord-014597-66vd2mdu	1779	18	tool	tool	NN
cord-014597-66vd2mdu	1779	19	to	to	TO
cord-014597-66vd2mdu	1779	20	knockdown	knockdown	VB
cord-014597-66vd2mdu	1779	21	miRNA	miRNA	NNP
cord-014597-66vd2mdu	1779	22	expression	expression	NN
cord-014597-66vd2mdu	1779	23	in	in	IN
cord-014597-66vd2mdu	1779	24	CHO	CHO	NNP
cord-014597-66vd2mdu	1779	25	cells	cell	NNS
cord-014597-66vd2mdu	1779	26	.	.	.
cord-014597-66vd2mdu	1780	1	The	the	DT
cord-014597-66vd2mdu	1780	2	data	datum	NNS
cord-014597-66vd2mdu	1780	3	was	be	VBD
cord-014597-66vd2mdu	1780	4	generated	generate	VBN
cord-014597-66vd2mdu	1780	5	using	use	VBG
cord-014597-66vd2mdu	1780	6	mixed	mixed	JJ
cord-014597-66vd2mdu	1780	7	pools	pool	NNS
cord-014597-66vd2mdu	1780	8	and	and	CC
cord-014597-66vd2mdu	1780	9	it	-PRON-	PRP
cord-014597-66vd2mdu	1780	10	remains	remain	VBZ
cord-014597-66vd2mdu	1780	11	to	to	TO
cord-014597-66vd2mdu	1780	12	be	be	VB
cord-014597-66vd2mdu	1780	13	established	establish	VBN
cord-014597-66vd2mdu	1780	14	if	if	IN
cord-014597-66vd2mdu	1780	15	both	both	DT
cord-014597-66vd2mdu	1780	16	alleles	allele	NNS
cord-014597-66vd2mdu	1780	17	can	can	MD
cord-014597-66vd2mdu	1780	18	be	be	VB
cord-014597-66vd2mdu	1780	19	successfully	successfully	RB
cord-014597-66vd2mdu	1780	20	targeted	target	VBN
cord-014597-66vd2mdu	1780	21	e.g.	e.g.	RB
cord-014597-66vd2mdu	1780	22	using	use	VBG
cord-014597-66vd2mdu	1780	23	nextgeneration	nextgeneration	NN
cord-014597-66vd2mdu	1780	24	sequencing	sequencing	NN
cord-014597-66vd2mdu	1780	25	of	of	IN
cord-014597-66vd2mdu	1780	26	individual	individual	JJ
cord-014597-66vd2mdu	1780	27	clones	clone	NNS
cord-014597-66vd2mdu	1780	28	.	.	.
cord-014597-66vd2mdu	1781	1	Background	background	NN
cord-014597-66vd2mdu	1781	2	Chinese	chinese	JJ
cord-014597-66vd2mdu	1781	3	hamster	hamster	NN
cord-014597-66vd2mdu	1781	4	ovary	ovary	NN
cord-014597-66vd2mdu	1781	5	(	(	-LRB-
cord-014597-66vd2mdu	1781	6	CHO	CHO	NNP
cord-014597-66vd2mdu	1781	7	)	)	-RRB-
cord-014597-66vd2mdu	1781	8	cells	cell	NNS
cord-014597-66vd2mdu	1781	9	are	be	VBP
cord-014597-66vd2mdu	1781	10	the	the	DT
cord-014597-66vd2mdu	1781	11	most	most	RBS
cord-014597-66vd2mdu	1781	12	widely	widely	RB
cord-014597-66vd2mdu	1781	13	used	use	VBN
cord-014597-66vd2mdu	1781	14	host	host	NN
cord-014597-66vd2mdu	1781	15	cell	cell	NN
cord-014597-66vd2mdu	1781	16	line	line	NN
cord-014597-66vd2mdu	1781	17	for	for	IN
cord-014597-66vd2mdu	1781	18	the	the	DT
cord-014597-66vd2mdu	1781	19	production	production	NN
cord-014597-66vd2mdu	1781	20	of	of	IN
cord-014597-66vd2mdu	1781	21	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1781	22	antibodies	antibody	NNS
cord-014597-66vd2mdu	1781	23	.	.	.
cord-014597-66vd2mdu	1782	1	Pre	pre	JJ
cord-014597-66vd2mdu	1782	2	-	-	JJ
cord-014597-66vd2mdu	1782	3	and	and	CC
cord-014597-66vd2mdu	1782	4	posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	1782	5	modifications	modification	NNS
cord-014597-66vd2mdu	1782	6	and	and	CC
cord-014597-66vd2mdu	1782	7	optimization	optimization	NN
cord-014597-66vd2mdu	1782	8	of	of	IN
cord-014597-66vd2mdu	1782	9	culture	culture	NN
cord-014597-66vd2mdu	1782	10	methods	method	NNS
cord-014597-66vd2mdu	1782	11	contributed	contribute	VBD
cord-014597-66vd2mdu	1782	12	to	to	TO
cord-014597-66vd2mdu	1782	13	increase	increase	VB
cord-014597-66vd2mdu	1782	14	the	the	DT
cord-014597-66vd2mdu	1782	15	productivity	productivity	NN
cord-014597-66vd2mdu	1782	16	,	,	,
cord-014597-66vd2mdu	1782	17	resulting	result	VBG
cord-014597-66vd2mdu	1782	18	in	in	IN
cord-014597-66vd2mdu	1782	19	a	a	DT
cord-014597-66vd2mdu	1782	20	very	very	RB
cord-014597-66vd2mdu	1782	21	high	high	JJ
cord-014597-66vd2mdu	1782	22	titre	titre	NN
cord-014597-66vd2mdu	1782	23	[	[	-LRB-
cord-014597-66vd2mdu	1782	24	1	1	CD
cord-014597-66vd2mdu	1782	25	,	,	,
cord-014597-66vd2mdu	1782	26	2	2	CD
cord-014597-66vd2mdu	1782	27	]	]	-RRB-
cord-014597-66vd2mdu	1782	28	.	.	.
cord-014597-66vd2mdu	1783	1	However	however	RB
cord-014597-66vd2mdu	1783	2	,	,	,
cord-014597-66vd2mdu	1783	3	it	-PRON-	PRP
cord-014597-66vd2mdu	1783	4	has	have	VBZ
cord-014597-66vd2mdu	1783	5	been	be	VBN
cord-014597-66vd2mdu	1783	6	pointed	point	VBN
cord-014597-66vd2mdu	1783	7	out	out	RP
cord-014597-66vd2mdu	1783	8	that	that	IN
cord-014597-66vd2mdu	1783	9	the	the	DT
cord-014597-66vd2mdu	1783	10	intracellular	intracellular	JJ
cord-014597-66vd2mdu	1783	11	secretion	secretion	NN
cord-014597-66vd2mdu	1783	12	process	process	NN
cord-014597-66vd2mdu	1783	13	is	be	VBZ
cord-014597-66vd2mdu	1783	14	a	a	DT
cord-014597-66vd2mdu	1783	15	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	1783	16	in	in	IN
cord-014597-66vd2mdu	1783	17	the	the	DT
cord-014597-66vd2mdu	1783	18	production	production	NN
cord-014597-66vd2mdu	1783	19	of	of	IN
cord-014597-66vd2mdu	1783	20	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1783	21	antibodies	antibody	NNS
cord-014597-66vd2mdu	1783	22	[	[	-LRB-
cord-014597-66vd2mdu	1783	23	3	3	CD
cord-014597-66vd2mdu	1783	24	]	]	-RRB-
cord-014597-66vd2mdu	1783	25	.	.	.
cord-014597-66vd2mdu	1784	1	In	in	IN
cord-014597-66vd2mdu	1784	2	addition	addition	NN
cord-014597-66vd2mdu	1784	3	,	,	,
cord-014597-66vd2mdu	1784	4	the	the	DT
cord-014597-66vd2mdu	1784	5	details	detail	NNS
cord-014597-66vd2mdu	1784	6	of	of	IN
cord-014597-66vd2mdu	1784	7	the	the	DT
cord-014597-66vd2mdu	1784	8	process	process	NN
cord-014597-66vd2mdu	1784	9	of	of	IN
cord-014597-66vd2mdu	1784	10	secretion	secretion	NN
cord-014597-66vd2mdu	1784	11	of	of	IN
cord-014597-66vd2mdu	1784	12	humanized	humanize	VBN
cord-014597-66vd2mdu	1784	13	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1784	14	antibodies	antibody	NNS
cord-014597-66vd2mdu	1784	15	from	from	IN
cord-014597-66vd2mdu	1784	16	CHO	CHO	NNP
cord-014597-66vd2mdu	1784	17	cells	cell	NNS
cord-014597-66vd2mdu	1784	18	have	have	VBP
cord-014597-66vd2mdu	1784	19	not	not	RB
cord-014597-66vd2mdu	1784	20	been	be	VBN
cord-014597-66vd2mdu	1784	21	well	well	RB
cord-014597-66vd2mdu	1784	22	investigated	investigate	VBN
cord-014597-66vd2mdu	1784	23	.	.	.
cord-014597-66vd2mdu	1785	1	In	in	IN
cord-014597-66vd2mdu	1785	2	this	this	DT
cord-014597-66vd2mdu	1785	3	study	study	NN
cord-014597-66vd2mdu	1785	4	,	,	,
cord-014597-66vd2mdu	1785	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1785	6	thus	thus	RB
cord-014597-66vd2mdu	1785	7	analysed	analyse	VBD
cord-014597-66vd2mdu	1785	8	the	the	DT
cord-014597-66vd2mdu	1785	9	detailed	detailed	JJ
cord-014597-66vd2mdu	1785	10	process	process	NN
cord-014597-66vd2mdu	1785	11	of	of	IN
cord-014597-66vd2mdu	1785	12	secretion	secretion	NN
cord-014597-66vd2mdu	1785	13	of	of	IN
cord-014597-66vd2mdu	1785	14	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1785	15	antibodies	antibody	NNS
cord-014597-66vd2mdu	1785	16	using	use	VBG
cord-014597-66vd2mdu	1785	17	CHO	CHO	NNP
cord-014597-66vd2mdu	1785	18	cell	cell	NN
cord-014597-66vd2mdu	1785	19	lines	line	NNS
cord-014597-66vd2mdu	1785	20	,	,	,
cord-014597-66vd2mdu	1785	21	which	which	WDT
cord-014597-66vd2mdu	1785	22	have	have	VBP
cord-014597-66vd2mdu	1785	23	already	already	RB
cord-014597-66vd2mdu	1785	24	been	be	VBN
cord-014597-66vd2mdu	1785	25	established	establish	VBN
cord-014597-66vd2mdu	1785	26	as	as	IN
cord-014597-66vd2mdu	1785	27	high	high	JJ
cord-014597-66vd2mdu	1785	28	producers	producer	NNS
cord-014597-66vd2mdu	1785	29	,	,	,
cord-014597-66vd2mdu	1785	30	with	with	IN
cord-014597-66vd2mdu	1785	31	the	the	DT
cord-014597-66vd2mdu	1785	32	aim	aim	NN
cord-014597-66vd2mdu	1785	33	of	of	IN
cord-014597-66vd2mdu	1785	34	obtaining	obtain	VBG
cord-014597-66vd2mdu	1785	35	information	information	NN
cord-014597-66vd2mdu	1785	36	for	for	IN
cord-014597-66vd2mdu	1785	37	the	the	DT
cord-014597-66vd2mdu	1785	38	more	more	RBR
cord-014597-66vd2mdu	1785	39	rational	rational	JJ
cord-014597-66vd2mdu	1785	40	and	and	CC
cord-014597-66vd2mdu	1785	41	efficient	efficient	JJ
cord-014597-66vd2mdu	1785	42	establishment	establishment	NN
cord-014597-66vd2mdu	1785	43	of	of	IN
cord-014597-66vd2mdu	1785	44	high	high	JJ
cord-014597-66vd2mdu	1785	45	-	-	HYPH
cord-014597-66vd2mdu	1785	46	producer	producer	NN
cord-014597-66vd2mdu	1785	47	cells	cell	NNS
cord-014597-66vd2mdu	1785	48	.	.	.
cord-014597-66vd2mdu	1786	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1786	2	performed	perform	VBD
cord-014597-66vd2mdu	1786	3	1	1	CD
cord-014597-66vd2mdu	1786	4	)	)	-RRB-
cord-014597-66vd2mdu	1786	5	chase	chase	NN
cord-014597-66vd2mdu	1786	6	assay	assay	NN
cord-014597-66vd2mdu	1786	7	,	,	,
cord-014597-66vd2mdu	1786	8	2	2	CD
cord-014597-66vd2mdu	1786	9	)	)	-RRB-
cord-014597-66vd2mdu	1786	10	immunofluorescent	immunofluorescent	NNP
cord-014597-66vd2mdu	1786	11	microscopy	microscopy	NNP
cord-014597-66vd2mdu	1786	12	observation	observation	NN
cord-014597-66vd2mdu	1786	13	,	,	,
cord-014597-66vd2mdu	1786	14	and	and	CC
cord-014597-66vd2mdu	1786	15	3	3	LS
cord-014597-66vd2mdu	1786	16	)	)	-RRB-
cord-014597-66vd2mdu	1786	17	size	size	NN
cord-014597-66vd2mdu	1786	18	exclusion	exclusion	NN
cord-014597-66vd2mdu	1786	19	chromatography	chromatography	NN
cord-014597-66vd2mdu	1786	20	(	(	-LRB-
cord-014597-66vd2mdu	1786	21	SEC	SEC	NNP
cord-014597-66vd2mdu	1786	22	)	)	-RRB-
cord-014597-66vd2mdu	1786	23	analysis	analysis	NN
cord-014597-66vd2mdu	1786	24	to	to	TO
cord-014597-66vd2mdu	1786	25	investigate	investigate	VB
cord-014597-66vd2mdu	1786	26	the	the	DT
cord-014597-66vd2mdu	1786	27	duration	duration	NN
cord-014597-66vd2mdu	1786	28	of	of	IN
cord-014597-66vd2mdu	1786	29	secretion	secretion	NN
cord-014597-66vd2mdu	1786	30	,	,	,
cord-014597-66vd2mdu	1786	31	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	1786	32	position	position	NN
cord-014597-66vd2mdu	1786	33	,	,	,
cord-014597-66vd2mdu	1786	34	and	and	CC
cord-014597-66vd2mdu	1786	35	formation	formation	NN
cord-014597-66vd2mdu	1786	36	of	of	IN
cord-014597-66vd2mdu	1786	37	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1786	38	IgG	IgG	NNP
cord-014597-66vd2mdu	1786	39	,	,	,
cord-014597-66vd2mdu	1786	40	respectively	respectively	RB
cord-014597-66vd2mdu	1786	41	.	.	.
cord-014597-66vd2mdu	1787	1	High	high	JJ
cord-014597-66vd2mdu	1787	2	-	-	HYPH
cord-014597-66vd2mdu	1787	3	producer	producer	NN
cord-014597-66vd2mdu	1787	4	CHO	CHO	NNP
cord-014597-66vd2mdu	1787	5	cells	cell	NNS
cord-014597-66vd2mdu	1787	6	expressing	express	VBG
cord-014597-66vd2mdu	1787	7	humanized	humanize	VBN
cord-014597-66vd2mdu	1787	8	IgG1	igg1	NN
cord-014597-66vd2mdu	1788	1	[	[	-LRB-
cord-014597-66vd2mdu	1788	2	4	4	CD
cord-014597-66vd2mdu	1788	3	]	]	-RRB-
cord-014597-66vd2mdu	1788	4	and	and	CC
cord-014597-66vd2mdu	1788	5	IgG3	IgG3	NNP
cord-014597-66vd2mdu	1788	6	were	be	VBD
cord-014597-66vd2mdu	1788	7	used	use	VBN
cord-014597-66vd2mdu	1788	8	.	.	.
cord-014597-66vd2mdu	1789	1	For	for	IN
cord-014597-66vd2mdu	1789	2	the	the	DT
cord-014597-66vd2mdu	1789	3	chase	chase	NN
cord-014597-66vd2mdu	1789	4	assay	assay	NN
cord-014597-66vd2mdu	1789	5	,	,	,
cord-014597-66vd2mdu	1789	6	cells	cell	NNS
cord-014597-66vd2mdu	1789	7	were	be	VBD
cord-014597-66vd2mdu	1789	8	cultivated	cultivate	VBN
cord-014597-66vd2mdu	1789	9	in	in	IN
cord-014597-66vd2mdu	1789	10	shake	shake	NN
cord-014597-66vd2mdu	1789	11	flasks	flask	NNS
cord-014597-66vd2mdu	1789	12	with	with	IN
cord-014597-66vd2mdu	1789	13	serum	serum	NN
cord-014597-66vd2mdu	1789	14	-	-	HYPH
cord-014597-66vd2mdu	1789	15	free	free	JJ
cord-014597-66vd2mdu	1789	16	medium	medium	NN
cord-014597-66vd2mdu	1789	17	containing	contain	VBG
cord-014597-66vd2mdu	1789	18	50	50	CD
cord-014597-66vd2mdu	1789	19	μg	μg	NNS
cord-014597-66vd2mdu	1789	20	/	/	SYM
cord-014597-66vd2mdu	1789	21	ml	ml	NN
cord-014597-66vd2mdu	1789	22	cycloheximide	cycloheximide	NN
cord-014597-66vd2mdu	1789	23	(	(	-LRB-
cord-014597-66vd2mdu	1789	24	CHX	CHX	NNP
cord-014597-66vd2mdu	1789	25	)	)	-RRB-
cord-014597-66vd2mdu	1789	26	to	to	TO
cord-014597-66vd2mdu	1789	27	stop	stop	VB
cord-014597-66vd2mdu	1789	28	nascent	nascent	JJ
cord-014597-66vd2mdu	1789	29	peptide	peptide	NN
cord-014597-66vd2mdu	1789	30	synthesis	synthesis	NN
cord-014597-66vd2mdu	1789	31	.	.	.
cord-014597-66vd2mdu	1790	1	The	the	DT
cord-014597-66vd2mdu	1790	2	amounts	amount	NNS
cord-014597-66vd2mdu	1790	3	of	of	IN
cord-014597-66vd2mdu	1790	4	IgG	igg	NN
cord-014597-66vd2mdu	1790	5	both	both	DT
cord-014597-66vd2mdu	1790	6	remaining	remain	VBG
cord-014597-66vd2mdu	1790	7	in	in	IN
cord-014597-66vd2mdu	1790	8	the	the	DT
cord-014597-66vd2mdu	1790	9	cell	cell	NN
cord-014597-66vd2mdu	1790	10	and	and	CC
cord-014597-66vd2mdu	1790	11	secreted	secrete	VBD
cord-014597-66vd2mdu	1790	12	into	into	IN
cord-014597-66vd2mdu	1790	13	the	the	DT
cord-014597-66vd2mdu	1790	14	medium	medium	NN
cord-014597-66vd2mdu	1790	15	at	at	IN
cord-014597-66vd2mdu	1790	16	each	each	DT
cord-014597-66vd2mdu	1790	17	time	time	NN
cord-014597-66vd2mdu	1790	18	point	point	NN
cord-014597-66vd2mdu	1790	19	were	be	VBD
cord-014597-66vd2mdu	1790	20	measured	measure	VBN
cord-014597-66vd2mdu	1790	21	by	by	IN
cord-014597-66vd2mdu	1790	22	quantitative	quantitative	JJ
cord-014597-66vd2mdu	1790	23	western	western	JJ
cord-014597-66vd2mdu	1790	24	blotting	blotting	NN
cord-014597-66vd2mdu	1790	25	.	.	.
cord-014597-66vd2mdu	1791	1	For	for	IN
cord-014597-66vd2mdu	1791	2	immunofluorescent	immunofluorescent	NNP
cord-014597-66vd2mdu	1791	3	microscopy	microscopy	NNP
cord-014597-66vd2mdu	1791	4	observation	observation	NN
cord-014597-66vd2mdu	1791	5	,	,	,
cord-014597-66vd2mdu	1791	6	cells	cell	NNS
cord-014597-66vd2mdu	1791	7	were	be	VBD
cord-014597-66vd2mdu	1791	8	cultivated	cultivate	VBN
cord-014597-66vd2mdu	1791	9	on	on	IN
cord-014597-66vd2mdu	1791	10	coverslips	coverslip	NNS
cord-014597-66vd2mdu	1791	11	with	with	IN
cord-014597-66vd2mdu	1791	12	CHX	CHX	NNP
cord-014597-66vd2mdu	1791	13	for	for	IN
cord-014597-66vd2mdu	1791	14	4	4	CD
cord-014597-66vd2mdu	1791	15	h.	h.	NNP
cord-014597-66vd2mdu	1791	16	Immunofluorescent	Immunofluorescent	NNP
cord-014597-66vd2mdu	1791	17	staining	stain	VBG
cord-014597-66vd2mdu	1791	18	against	against	IN
cord-014597-66vd2mdu	1791	19	the	the	DT
cord-014597-66vd2mdu	1791	20	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1791	21	IgG	igg	NN
cord-014597-66vd2mdu	1791	22	,	,	,
cord-014597-66vd2mdu	1791	23	endoplasmic	endoplasmic	NN
cord-014597-66vd2mdu	1791	24	reticulum	reticulum	NN
cord-014597-66vd2mdu	1791	25	(	(	-LRB-
cord-014597-66vd2mdu	1791	26	ER	ER	NNP
cord-014597-66vd2mdu	1791	27	)	)	-RRB-
cord-014597-66vd2mdu	1791	28	,	,	,
cord-014597-66vd2mdu	1791	29	and	and	CC
cord-014597-66vd2mdu	1791	30	Golgi	Golgi	NNP
cord-014597-66vd2mdu	1791	31	apparatus	apparatus	NN
cord-014597-66vd2mdu	1791	32	was	be	VBD
cord-014597-66vd2mdu	1791	33	performed	perform	VBN
cord-014597-66vd2mdu	1791	34	after	after	IN
cord-014597-66vd2mdu	1791	35	chemical	chemical	JJ
cord-014597-66vd2mdu	1791	36	fixation	fixation	NN
cord-014597-66vd2mdu	1791	37	.	.	.
cord-014597-66vd2mdu	1792	1	For	for	IN
cord-014597-66vd2mdu	1792	2	SEC	SEC	NNP
cord-014597-66vd2mdu	1792	3	,	,	,
cord-014597-66vd2mdu	1792	4	cells	cell	NNS
cord-014597-66vd2mdu	1792	5	cultured	culture	VBN
cord-014597-66vd2mdu	1792	6	with	with	IN
cord-014597-66vd2mdu	1792	7	CHX	CHX	NNP
cord-014597-66vd2mdu	1792	8	were	be	VBD
cord-014597-66vd2mdu	1792	9	re	re	VBN
cord-014597-66vd2mdu	1792	10	-	-	VBN
cord-014597-66vd2mdu	1792	11	suspended	suspend	VBN
cord-014597-66vd2mdu	1792	12	in	in	IN
cord-014597-66vd2mdu	1792	13	a	a	DT
cord-014597-66vd2mdu	1792	14	buffer	buffer	NN
cord-014597-66vd2mdu	1792	15	containing	contain	VBG
cord-014597-66vd2mdu	1792	16	tritonX-100	tritonX-100	NNS
cord-014597-66vd2mdu	1792	17	and	and	CC
cord-014597-66vd2mdu	1792	18	injected	inject	VBD
cord-014597-66vd2mdu	1792	19	into	into	IN
cord-014597-66vd2mdu	1792	20	a	a	DT
cord-014597-66vd2mdu	1792	21	column	column	NN
cord-014597-66vd2mdu	1792	22	.	.	.
cord-014597-66vd2mdu	1793	1	The	the	DT
cord-014597-66vd2mdu	1793	2	amount	amount	NN
cord-014597-66vd2mdu	1793	3	of	of	IN
cord-014597-66vd2mdu	1793	4	IgG	igg	NN
cord-014597-66vd2mdu	1793	5	in	in	IN
cord-014597-66vd2mdu	1793	6	each	each	DT
cord-014597-66vd2mdu	1793	7	fraction	fraction	NN
cord-014597-66vd2mdu	1793	8	was	be	VBD
cord-014597-66vd2mdu	1793	9	measured	measure	VBN
cord-014597-66vd2mdu	1793	10	by	by	IN
cord-014597-66vd2mdu	1793	11	quantitative	quantitative	JJ
cord-014597-66vd2mdu	1793	12	western	western	JJ
cord-014597-66vd2mdu	1793	13	blotting	blotting	NN
cord-014597-66vd2mdu	1793	14	.	.	.
cord-014597-66vd2mdu	1794	1	The	the	DT
cord-014597-66vd2mdu	1794	2	amount	amount	NN
cord-014597-66vd2mdu	1794	3	of	of	IN
cord-014597-66vd2mdu	1794	4	IgG3	igg3	NN
cord-014597-66vd2mdu	1794	5	in	in	IN
cord-014597-66vd2mdu	1794	6	the	the	DT
cord-014597-66vd2mdu	1794	7	supernatant	supernatant	NN
cord-014597-66vd2mdu	1794	8	increased	increase	VBD
cord-014597-66vd2mdu	1794	9	until	until	IN
cord-014597-66vd2mdu	1794	10	4	4	CD
cord-014597-66vd2mdu	1794	11	-	-	SYM
cord-014597-66vd2mdu	1794	12	6	6	CD
cord-014597-66vd2mdu	1794	13	h	h	NN
cord-014597-66vd2mdu	1794	14	after	after	IN
cord-014597-66vd2mdu	1794	15	the	the	DT
cord-014597-66vd2mdu	1794	16	inhibition	inhibition	NN
cord-014597-66vd2mdu	1794	17	of	of	IN
cord-014597-66vd2mdu	1794	18	protein	protein	NN
cord-014597-66vd2mdu	1794	19	synthesis	synthesis	NN
cord-014597-66vd2mdu	1794	20	by	by	IN
cord-014597-66vd2mdu	1794	21	CHX	CHX	NNP
cord-014597-66vd2mdu	1794	22	;	;	:
cord-014597-66vd2mdu	1794	23	however	however	RB
cord-014597-66vd2mdu	1794	24	,	,	,
cord-014597-66vd2mdu	1794	25	it	-PRON-	PRP
cord-014597-66vd2mdu	1794	26	hardly	hardly	RB
cord-014597-66vd2mdu	1794	27	changed	change	VBD
cord-014597-66vd2mdu	1794	28	thereafter	thereafter	RB
cord-014597-66vd2mdu	1795	1	(	(	-LRB-
cord-014597-66vd2mdu	1795	2	Fig	Fig	NNP
cord-014597-66vd2mdu	1795	3	.	.	NNP
cord-014597-66vd2mdu	1795	4	1	1	CD
cord-014597-66vd2mdu	1795	5	,	,	,
cord-014597-66vd2mdu	1795	6	upper	upper	JJ
cord-014597-66vd2mdu	1795	7	panel	panel	NN
cord-014597-66vd2mdu	1795	8	)	)	-RRB-
cord-014597-66vd2mdu	1795	9	.	.	.
cord-014597-66vd2mdu	1796	1	At	at	IN
cord-014597-66vd2mdu	1796	2	this	this	DT
cord-014597-66vd2mdu	1796	3	point	point	NN
cord-014597-66vd2mdu	1796	4	in	in	IN
cord-014597-66vd2mdu	1796	5	time	time	NN
cord-014597-66vd2mdu	1796	6	,	,	,
cord-014597-66vd2mdu	1796	7	however	however	RB
cord-014597-66vd2mdu	1796	8	,	,	,
cord-014597-66vd2mdu	1796	9	around	around	RB
cord-014597-66vd2mdu	1796	10	40	40	CD
cord-014597-66vd2mdu	1796	11	%	%	NN
cord-014597-66vd2mdu	1796	12	of	of	IN
cord-014597-66vd2mdu	1796	13	IgG	IgG	NNP
cord-014597-66vd2mdu	1796	14	still	still	RB
cord-014597-66vd2mdu	1796	15	remained	remain	VBD
cord-014597-66vd2mdu	1796	16	in	in	IN
cord-014597-66vd2mdu	1796	17	the	the	DT
cord-014597-66vd2mdu	1796	18	cells	cell	NNS
cord-014597-66vd2mdu	1796	19	(	(	-LRB-
cord-014597-66vd2mdu	1796	20	Fig	Fig	NNP
cord-014597-66vd2mdu	1796	21	.	.	NNP
cord-014597-66vd2mdu	1796	22	1	1	CD
cord-014597-66vd2mdu	1796	23	,	,	,
cord-014597-66vd2mdu	1796	24	lower	low	JJR
cord-014597-66vd2mdu	1796	25	panel	panel	NN
cord-014597-66vd2mdu	1796	26	)	)	-RRB-
cord-014597-66vd2mdu	1796	27	,	,	,
cord-014597-66vd2mdu	1796	28	meaning	mean	VBG
cord-014597-66vd2mdu	1796	29	that	that	IN
cord-014597-66vd2mdu	1796	30	all	all	DT
cord-014597-66vd2mdu	1796	31	of	of	IN
cord-014597-66vd2mdu	1796	32	the	the	DT
cord-014597-66vd2mdu	1796	33	synthesized	synthesize	VBN
cord-014597-66vd2mdu	1796	34	IgG	igg	NN
cord-014597-66vd2mdu	1796	35	could	could	MD
cord-014597-66vd2mdu	1796	36	not	not	RB
cord-014597-66vd2mdu	1796	37	be	be	VB
cord-014597-66vd2mdu	1796	38	secreted	secrete	VBN
cord-014597-66vd2mdu	1796	39	into	into	IN
cord-014597-66vd2mdu	1796	40	the	the	DT
cord-014597-66vd2mdu	1796	41	medium	medium	NN
cord-014597-66vd2mdu	1796	42	and	and	CC
cord-014597-66vd2mdu	1796	43	remained	remain	VBD
cord-014597-66vd2mdu	1796	44	in	in	IN
cord-014597-66vd2mdu	1796	45	the	the	DT
cord-014597-66vd2mdu	1796	46	cells	cell	NNS
cord-014597-66vd2mdu	1796	47	for	for	IN
cord-014597-66vd2mdu	1796	48	several	several	JJ
cord-014597-66vd2mdu	1796	49	hours	hour	NNS
cord-014597-66vd2mdu	1796	50	.	.	.
cord-014597-66vd2mdu	1797	1	This	this	DT
cord-014597-66vd2mdu	1797	2	result	result	NN
cord-014597-66vd2mdu	1797	3	was	be	VBD
cord-014597-66vd2mdu	1797	4	almost	almost	RB
cord-014597-66vd2mdu	1797	5	the	the	DT
cord-014597-66vd2mdu	1797	6	same	same	JJ
cord-014597-66vd2mdu	1797	7	as	as	IN
cord-014597-66vd2mdu	1797	8	that	that	DT
cord-014597-66vd2mdu	1797	9	of	of	IN
cord-014597-66vd2mdu	1797	10	studies	study	NNS
cord-014597-66vd2mdu	1797	11	using	use	VBG
cord-014597-66vd2mdu	1797	12	IgG1-expressing	igg1-expressing	NN
cord-014597-66vd2mdu	1797	13	cells	cell	NNS
cord-014597-66vd2mdu	1797	14	[	[	-LRB-
cord-014597-66vd2mdu	1797	15	5	5	CD
cord-014597-66vd2mdu	1797	16	,	,	,
cord-014597-66vd2mdu	1797	17	6	6	CD
cord-014597-66vd2mdu	1797	18	]	]	-RRB-
cord-014597-66vd2mdu	1797	19	.	.	.
cord-014597-66vd2mdu	1798	1	The	the	DT
cord-014597-66vd2mdu	1798	2	localization	localization	NN
cord-014597-66vd2mdu	1798	3	of	of	IN
cord-014597-66vd2mdu	1798	4	IgG	igg	NN
cord-014597-66vd2mdu	1798	5	in	in	IN
cord-014597-66vd2mdu	1798	6	the	the	DT
cord-014597-66vd2mdu	1798	7	cells	cell	NNS
cord-014597-66vd2mdu	1798	8	was	be	VBD
cord-014597-66vd2mdu	1798	9	checked	check	VBN
cord-014597-66vd2mdu	1798	10	before	before	IN
cord-014597-66vd2mdu	1798	11	and	and	CC
cord-014597-66vd2mdu	1798	12	after	after	IN
cord-014597-66vd2mdu	1798	13	the	the	DT
cord-014597-66vd2mdu	1798	14	addition	addition	NN
cord-014597-66vd2mdu	1798	15	of	of	IN
cord-014597-66vd2mdu	1798	16	CHX	CHX	NNP
cord-014597-66vd2mdu	1798	17	,	,	,
cord-014597-66vd2mdu	1798	18	with	with	IN
cord-014597-66vd2mdu	1798	19	the	the	DT
cord-014597-66vd2mdu	1798	20	results	result	NNS
cord-014597-66vd2mdu	1798	21	showing	show	VBG
cord-014597-66vd2mdu	1798	22	that	that	IN
cord-014597-66vd2mdu	1798	23	IgG1	IgG1	NNP
cord-014597-66vd2mdu	1798	24	remained	remain	VBD
cord-014597-66vd2mdu	1798	25	in	in	IN
cord-014597-66vd2mdu	1798	26	the	the	DT
cord-014597-66vd2mdu	1798	27	ER	er	NN
cord-014597-66vd2mdu	1798	28	and	and	CC
cord-014597-66vd2mdu	1798	29	was	be	VBD
cord-014597-66vd2mdu	1798	30	hardly	hardly	RB
cord-014597-66vd2mdu	1798	31	seen	see	VBN
cord-014597-66vd2mdu	1798	32	in	in	IN
cord-014597-66vd2mdu	1798	33	the	the	DT
cord-014597-66vd2mdu	1798	34	Golgi	Golgi	NNP
cord-014597-66vd2mdu	1798	35	apparatus	apparatus	NN
cord-014597-66vd2mdu	1798	36	[	[	-LRB-
cord-014597-66vd2mdu	1798	37	5	5	CD
cord-014597-66vd2mdu	1798	38	]	]	-RRB-
cord-014597-66vd2mdu	1798	39	[	[	-LRB-
cord-014597-66vd2mdu	1798	40	6	6	CD
cord-014597-66vd2mdu	1798	41	]	]	-RRB-
cord-014597-66vd2mdu	1798	42	[	[	-LRB-
cord-014597-66vd2mdu	1798	43	7	7	CD
cord-014597-66vd2mdu	1798	44	]	]	-RRB-
cord-014597-66vd2mdu	1798	45	;	;	:
cord-014597-66vd2mdu	1798	46	IgG	IgG	NNP
cord-014597-66vd2mdu	1798	47	did	do	VBD
cord-014597-66vd2mdu	1798	48	not	not	RB
cord-014597-66vd2mdu	1798	49	seem	seem	VB
cord-014597-66vd2mdu	1798	50	to	to	TO
cord-014597-66vd2mdu	1798	51	be	be	VB
cord-014597-66vd2mdu	1798	52	efficiently	efficiently	RB
cord-014597-66vd2mdu	1798	53	transported	transport	VBN
cord-014597-66vd2mdu	1798	54	to	to	IN
cord-014597-66vd2mdu	1798	55	the	the	DT
cord-014597-66vd2mdu	1798	56	Golgi	Golgi	NNP
cord-014597-66vd2mdu	1798	57	apparatus	apparatus	NN
cord-014597-66vd2mdu	1798	58	.	.	.
cord-014597-66vd2mdu	1799	1	The	the	DT
cord-014597-66vd2mdu	1799	2	SEC	SEC	NNP
cord-014597-66vd2mdu	1799	3	experiment	experiment	NN
cord-014597-66vd2mdu	1799	4	showed	show	VBD
cord-014597-66vd2mdu	1799	5	that	that	IN
cord-014597-66vd2mdu	1799	6	most	most	JJS
cord-014597-66vd2mdu	1799	7	of	of	IN
cord-014597-66vd2mdu	1799	8	the	the	DT
cord-014597-66vd2mdu	1799	9	IgG1	IgG1	NNP
cord-014597-66vd2mdu	1799	10	remaining	remain	VBG
cord-014597-66vd2mdu	1799	11	in	in	IN
cord-014597-66vd2mdu	1799	12	the	the	DT
cord-014597-66vd2mdu	1799	13	cell	cell	NN
cord-014597-66vd2mdu	1799	14	seemed	seem	VBD
cord-014597-66vd2mdu	1799	15	to	to	TO
cord-014597-66vd2mdu	1799	16	form	form	VB
cord-014597-66vd2mdu	1799	17	full	full	JJ
cord-014597-66vd2mdu	1799	18	-	-	HYPH
cord-014597-66vd2mdu	1799	19	sized	sized	JJ
cord-014597-66vd2mdu	1799	20	antibodies	antibody	NNS
cord-014597-66vd2mdu	1799	21	[	[	-LRB-
cord-014597-66vd2mdu	1799	22	5	5	CD
cord-014597-66vd2mdu	1799	23	,	,	,
cord-014597-66vd2mdu	1799	24	6	6	CD
cord-014597-66vd2mdu	1799	25	]	]	-RRB-
cord-014597-66vd2mdu	1799	26	,	,	,
cord-014597-66vd2mdu	1799	27	but	but	CC
cord-014597-66vd2mdu	1799	28	it	-PRON-	PRP
cord-014597-66vd2mdu	1799	29	could	could	MD
cord-014597-66vd2mdu	1799	30	not	not	RB
cord-014597-66vd2mdu	1799	31	be	be	VB
cord-014597-66vd2mdu	1799	32	secreted	secrete	VBN
cord-014597-66vd2mdu	1799	33	despite	despite	IN
cord-014597-66vd2mdu	1799	34	this	this	DT
cord-014597-66vd2mdu	1799	35	.	.	.
cord-014597-66vd2mdu	1800	1	The	the	DT
cord-014597-66vd2mdu	1800	2	high	high	JJ
cord-014597-66vd2mdu	1800	3	-	-	HYPH
cord-014597-66vd2mdu	1800	4	producer	producer	NN
cord-014597-66vd2mdu	1800	5	cells	cell	NNS
cord-014597-66vd2mdu	1800	6	could	could	MD
cord-014597-66vd2mdu	1800	7	not	not	RB
cord-014597-66vd2mdu	1800	8	secrete	secrete	VB
cord-014597-66vd2mdu	1800	9	all	all	DT
cord-014597-66vd2mdu	1800	10	of	of	IN
cord-014597-66vd2mdu	1800	11	the	the	DT
cord-014597-66vd2mdu	1800	12	synthesized	synthesize	VBN
cord-014597-66vd2mdu	1800	13	IgG	igg	NN
cord-014597-66vd2mdu	1800	14	,	,	,
cord-014597-66vd2mdu	1800	15	and	and	CC
cord-014597-66vd2mdu	1800	16	around	around	RB
cord-014597-66vd2mdu	1800	17	40	40	CD
cord-014597-66vd2mdu	1800	18	%	%	NN
cord-014597-66vd2mdu	1800	19	of	of	IN
cord-014597-66vd2mdu	1800	20	IgG	igg	NN
cord-014597-66vd2mdu	1800	21	remained	remain	VBD
cord-014597-66vd2mdu	1800	22	in	in	IN
cord-014597-66vd2mdu	1800	23	the	the	DT
cord-014597-66vd2mdu	1800	24	cells	cell	NNS
cord-014597-66vd2mdu	1800	25	for	for	IN
cord-014597-66vd2mdu	1800	26	several	several	JJ
cord-014597-66vd2mdu	1800	27	hours	hour	NNS
cord-014597-66vd2mdu	1800	28	.	.	.
cord-014597-66vd2mdu	1801	1	This	this	DT
cord-014597-66vd2mdu	1801	2	incomplete	incomplete	JJ
cord-014597-66vd2mdu	1801	3	secretion	secretion	NN
cord-014597-66vd2mdu	1801	4	is	be	VBZ
cord-014597-66vd2mdu	1801	5	a	a	DT
cord-014597-66vd2mdu	1801	6	common	common	JJ
cord-014597-66vd2mdu	1801	7	phenomenon	phenomenon	NN
cord-014597-66vd2mdu	1801	8	among	among	IN
cord-014597-66vd2mdu	1801	9	CHO	CHO	NNP
cord-014597-66vd2mdu	1801	10	cells	cell	NNS
cord-014597-66vd2mdu	1801	11	producing	produce	VBG
cord-014597-66vd2mdu	1801	12	different	different	JJ
cord-014597-66vd2mdu	1801	13	types	type	NNS
cord-014597-66vd2mdu	1801	14	of	of	IN
cord-014597-66vd2mdu	1801	15	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1801	16	IgG.	IgG.	NNP
cord-014597-66vd2mdu	1802	1	The	the	DT
cord-014597-66vd2mdu	1802	2	IgG	igg	NN
cord-014597-66vd2mdu	1802	3	could	could	MD
cord-014597-66vd2mdu	1802	4	not	not	RB
cord-014597-66vd2mdu	1802	5	be	be	VB
cord-014597-66vd2mdu	1802	6	transported	transport	VBN
cord-014597-66vd2mdu	1802	7	from	from	IN
cord-014597-66vd2mdu	1802	8	the	the	DT
cord-014597-66vd2mdu	1802	9	ER	ER	NNP
cord-014597-66vd2mdu	1802	10	to	to	IN
cord-014597-66vd2mdu	1802	11	the	the	DT
cord-014597-66vd2mdu	1802	12	Golgi	Golgi	NNP
cord-014597-66vd2mdu	1802	13	despite	despite	IN
cord-014597-66vd2mdu	1802	14	its	-PRON-	PRP$
cord-014597-66vd2mdu	1802	15	formation	formation	NN
cord-014597-66vd2mdu	1802	16	of	of	IN
cord-014597-66vd2mdu	1802	17	fullsized	fullsized	JJ
cord-014597-66vd2mdu	1802	18	antibodies	antibody	NNS
cord-014597-66vd2mdu	1802	19	.	.	.
cord-014597-66vd2mdu	1803	1	Solving	solve	VBG
cord-014597-66vd2mdu	1803	2	this	this	DT
cord-014597-66vd2mdu	1803	3	bottleneck	bottleneck	NN
cord-014597-66vd2mdu	1803	4	in	in	IN
cord-014597-66vd2mdu	1803	5	the	the	DT
cord-014597-66vd2mdu	1803	6	transportation	transportation	NN
cord-014597-66vd2mdu	1803	7	of	of	IN
cord-014597-66vd2mdu	1803	8	IgG	igg	NN
cord-014597-66vd2mdu	1803	9	from	from	IN
cord-014597-66vd2mdu	1803	10	the	the	DT
cord-014597-66vd2mdu	1803	11	ER	ER	NNP
cord-014597-66vd2mdu	1803	12	to	to	IN
cord-014597-66vd2mdu	1803	13	the	the	DT
cord-014597-66vd2mdu	1803	14	Golgi	Golgi	NNP
cord-014597-66vd2mdu	1803	15	and/or	and/or	CC
cord-014597-66vd2mdu	1803	16	achieving	achieve	VBG
cord-014597-66vd2mdu	1803	17	more	more	RBR
cord-014597-66vd2mdu	1803	18	efficient	efficient	JJ
cord-014597-66vd2mdu	1803	19	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1803	20	of	of	IN
cord-014597-66vd2mdu	1803	21	IgG	igg	NN
cord-014597-66vd2mdu	1803	22	after	after	IN
cord-014597-66vd2mdu	1803	23	the	the	DT
cord-014597-66vd2mdu	1803	24	formation	formation	NN
cord-014597-66vd2mdu	1803	25	of	of	IN
cord-014597-66vd2mdu	1803	26	full	full	JJ
cord-014597-66vd2mdu	1803	27	-	-	HYPH
cord-014597-66vd2mdu	1803	28	sized	sized	JJ
cord-014597-66vd2mdu	1803	29	antibodies	antibody	NNS
cord-014597-66vd2mdu	1803	30	might	may	MD
cord-014597-66vd2mdu	1803	31	be	be	VB
cord-014597-66vd2mdu	1803	32	the	the	DT
cord-014597-66vd2mdu	1803	33	next	next	JJ
cord-014597-66vd2mdu	1803	34	target	target	NN
cord-014597-66vd2mdu	1803	35	to	to	TO
cord-014597-66vd2mdu	1803	36	improve	improve	VB
cord-014597-66vd2mdu	1803	37	productivity	productivity	NN
cord-014597-66vd2mdu	1803	38	.	.	.
cord-014597-66vd2mdu	1804	1	Background	background	NN
cord-014597-66vd2mdu	1804	2	Humanized	Humanized	NNP
cord-014597-66vd2mdu	1804	3	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	1804	4	antibodies	antibody	NNS
cord-014597-66vd2mdu	1804	5	(	(	-LRB-
cord-014597-66vd2mdu	1804	6	mAbs	mAbs	NNP
cord-014597-66vd2mdu	1804	7	)	)	-RRB-
cord-014597-66vd2mdu	1804	8	are	be	VBP
cord-014597-66vd2mdu	1804	9	among	among	IN
cord-014597-66vd2mdu	1804	10	the	the	DT
cord-014597-66vd2mdu	1804	11	most	most	RBS
cord-014597-66vd2mdu	1804	12	promising	promising	JJ
cord-014597-66vd2mdu	1804	13	drugs	drug	NNS
cord-014597-66vd2mdu	1804	14	,	,	,
cord-014597-66vd2mdu	1804	15	but	but	CC
cord-014597-66vd2mdu	1804	16	defined	define	VBN
cord-014597-66vd2mdu	1804	17	strategies	strategy	NNS
cord-014597-66vd2mdu	1804	18	for	for	IN
cord-014597-66vd2mdu	1804	19	their	-PRON-	PRP$
cord-014597-66vd2mdu	1804	20	modification	modification	NN
cord-014597-66vd2mdu	1804	21	are	be	VBP
cord-014597-66vd2mdu	1804	22	still	still	RB
cord-014597-66vd2mdu	1804	23	not	not	RB
cord-014597-66vd2mdu	1804	24	available	available	JJ
cord-014597-66vd2mdu	1804	25	.	.	.
cord-014597-66vd2mdu	1805	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	1805	2	work	work	NN
cord-014597-66vd2mdu	1805	3	deals	deal	NNS
cord-014597-66vd2mdu	1805	4	with	with	IN
cord-014597-66vd2mdu	1805	5	humanization	humanization	NN
cord-014597-66vd2mdu	1805	6	of	of	IN
cord-014597-66vd2mdu	1805	7	murine	murine	JJ
cord-014597-66vd2mdu	1805	8	mAb2/	mAb2/	NNS
cord-014597-66vd2mdu	1805	9	3H6	3h6	CD
cord-014597-66vd2mdu	1805	10	.	.	.
cord-014597-66vd2mdu	1806	1	The	the	DT
cord-014597-66vd2mdu	1806	2	superhumanization	superhumanization	NN
cord-014597-66vd2mdu	1806	3	approach	approach	NN
cord-014597-66vd2mdu	1806	4	leads	lead	VBZ
cord-014597-66vd2mdu	1806	5	to	to	IN
cord-014597-66vd2mdu	1806	6	a	a	DT
cord-014597-66vd2mdu	1806	7	loss	loss	NN
cord-014597-66vd2mdu	1806	8	of	of	IN
cord-014597-66vd2mdu	1806	9	binding	bind	VBG
cord-014597-66vd2mdu	1806	10	affinity	affinity	NN
cord-014597-66vd2mdu	1806	11	which	which	WDT
cord-014597-66vd2mdu	1806	12	was	be	VBD
cord-014597-66vd2mdu	1806	13	partially	partially	RB
cord-014597-66vd2mdu	1806	14	restored	restore	VBN
cord-014597-66vd2mdu	1806	15	by	by	IN
cord-014597-66vd2mdu	1806	16	a	a	DT
cord-014597-66vd2mdu	1806	17	single	single	JJ
cord-014597-66vd2mdu	1806	18	human	human	NN
cord-014597-66vd2mdu	1806	19	-	-	HYPH
cord-014597-66vd2mdu	1806	20	to	to	IN
cord-014597-66vd2mdu	1806	21	-	-	HYPH
cord-014597-66vd2mdu	1806	22	mouse	mouse	NN
cord-014597-66vd2mdu	1806	23	backmutation	backmutation	NN
cord-014597-66vd2mdu	1806	24	(	(	-LRB-
cord-014597-66vd2mdu	1806	25	T98hR	t98hr	NN
cord-014597-66vd2mdu	1806	26	)	)	-RRB-
cord-014597-66vd2mdu	1806	27	.	.	.
cord-014597-66vd2mdu	1807	1	[	[	-LRB-
cord-014597-66vd2mdu	1807	2	1	1	CD
cord-014597-66vd2mdu	1807	3	]	]	-RRB-
cord-014597-66vd2mdu	1808	1	This	this	DT
cord-014597-66vd2mdu	1808	2	residue	residue	NN
cord-014597-66vd2mdu	1808	3	was	be	VBD
cord-014597-66vd2mdu	1808	4	selected	select	VBN
cord-014597-66vd2mdu	1808	5	by	by	IN
cord-014597-66vd2mdu	1808	6	synergistic	synergistic	JJ
cord-014597-66vd2mdu	1808	7	combination	combination	NN
cord-014597-66vd2mdu	1808	8	of	of	IN
cord-014597-66vd2mdu	1808	9	sequence	sequence	NN
cord-014597-66vd2mdu	1808	10	analyses	analysis	NNS
cord-014597-66vd2mdu	1808	11	of	of	IN
cord-014597-66vd2mdu	1808	12	antibody	antibody	NN
cord-014597-66vd2mdu	1808	13	framework	framework	NN
cord-014597-66vd2mdu	1808	14	regions	region	NNS
cord-014597-66vd2mdu	1808	15	and	and	CC
cord-014597-66vd2mdu	1808	16	structural	structural	JJ
cord-014597-66vd2mdu	1808	17	information	information	NN
cord-014597-66vd2mdu	1808	18	using	use	VBG
cord-014597-66vd2mdu	1808	19	novel	novel	NN
cord-014597-66vd2mdu	1808	20	in	in	IN
cord-014597-66vd2mdu	1808	21	silico	silico	NN
cord-014597-66vd2mdu	1808	22	simulations	simulation	NNS
cord-014597-66vd2mdu	1808	23	.	.	.
cord-014597-66vd2mdu	1809	1	For	for	IN
cord-014597-66vd2mdu	1809	2	structural	structural	JJ
cord-014597-66vd2mdu	1809	3	stabilization	stabilization	NN
cord-014597-66vd2mdu	1809	4	,	,	,
cord-014597-66vd2mdu	1809	5	a	a	DT
cord-014597-66vd2mdu	1809	6	conglomeration	conglomeration	NN
cord-014597-66vd2mdu	1809	7	of	of	IN
cord-014597-66vd2mdu	1809	8	tyrosine	tyrosine	NN
cord-014597-66vd2mdu	1809	9	residues	residue	NNS
cord-014597-66vd2mdu	1809	10	surrounding	surround	VBG
cord-014597-66vd2mdu	1809	11	T98hR	t98hr	PRP
cord-014597-66vd2mdu	1809	12	was	be	VBD
cord-014597-66vd2mdu	1809	13	identified	identify	VBN
cord-014597-66vd2mdu	1809	14	,	,	,
cord-014597-66vd2mdu	1809	15	the	the	DT
cord-014597-66vd2mdu	1809	16	so	so	RB
cord-014597-66vd2mdu	1809	17	called	call	VBN
cord-014597-66vd2mdu	1809	18	"	"	``
cord-014597-66vd2mdu	1809	19	tyrosine	tyrosine	NN
cord-014597-66vd2mdu	1809	20	cage	cage	NN
cord-014597-66vd2mdu	1809	21	"	"	''
cord-014597-66vd2mdu	1809	22	.	.	.
cord-014597-66vd2mdu	1810	1	[	[	-LRB-
cord-014597-66vd2mdu	1810	2	2	2	LS
cord-014597-66vd2mdu	1810	3	]	]	-RRB-
cord-014597-66vd2mdu	1810	4	Analysis	Analysis	NNP
cord-014597-66vd2mdu	1810	5	of	of	IN
cord-014597-66vd2mdu	1810	6	the	the	DT
cord-014597-66vd2mdu	1810	7	"	"	``
cord-014597-66vd2mdu	1810	8	tyrosine	tyrosine	NN
cord-014597-66vd2mdu	1810	9	cage	cage	NN
cord-014597-66vd2mdu	1810	10	"	"	''
cord-014597-66vd2mdu	1810	11	was	be	VBD
cord-014597-66vd2mdu	1810	12	done	do	VBN
cord-014597-66vd2mdu	1810	13	by	by	IN
cord-014597-66vd2mdu	1810	14	alanine	alanine	NN
cord-014597-66vd2mdu	1810	15	scanning	scanning	NN
cord-014597-66vd2mdu	1810	16	mutations	mutation	NNS
cord-014597-66vd2mdu	1810	17	with	with	IN
cord-014597-66vd2mdu	1810	18	a	a	DT
cord-014597-66vd2mdu	1810	19	double	double	JJ
cord-014597-66vd2mdu	1810	20	mutation	mutation	NN
cord-014597-66vd2mdu	1810	21	variant	variant	NN
cord-014597-66vd2mdu	1810	22	T98hR	t98hr	NN
cord-014597-66vd2mdu	1810	23	+	+	CC
cord-014597-66vd2mdu	1810	24	Y27hA	y27ha	NN
cord-014597-66vd2mdu	1810	25	(	(	-LRB-
cord-014597-66vd2mdu	1810	26	BM09	BM09	NNP
cord-014597-66vd2mdu	1810	27	)	)	-RRB-
cord-014597-66vd2mdu	1810	28	and	and	CC
cord-014597-66vd2mdu	1810	29	a	a	DT
cord-014597-66vd2mdu	1810	30	triple	triple	JJ
cord-014597-66vd2mdu	1810	31	mutation	mutation	NN
cord-014597-66vd2mdu	1810	32	variant	variant	NN
cord-014597-66vd2mdu	1810	33	T98hR	t98hr	XX
cord-014597-66vd2mdu	1811	1	+	+	CC
cord-014597-66vd2mdu	1812	1	Y27hA	y27ha	NN
cord-014597-66vd2mdu	1813	1	+	+	CC
cord-014597-66vd2mdu	1814	1	Y32hA	y32ha	NN
cord-014597-66vd2mdu	1814	2	(	(	-LRB-
cord-014597-66vd2mdu	1814	3	BM10	BM10	NNP
cord-014597-66vd2mdu	1814	4	)	)	-RRB-
cord-014597-66vd2mdu	1814	5	.	.	.
cord-014597-66vd2mdu	1815	1	In	in	IN
cord-014597-66vd2mdu	1815	2	a	a	DT
cord-014597-66vd2mdu	1815	3	recent	recent	JJ
cord-014597-66vd2mdu	1815	4	series	series	NN
cord-014597-66vd2mdu	1815	5	of	of	IN
cord-014597-66vd2mdu	1815	6	experiments	experiment	NNS
cord-014597-66vd2mdu	1815	7	we	-PRON-	PRP
cord-014597-66vd2mdu	1815	8	tried	try	VBD
cord-014597-66vd2mdu	1815	9	to	to	TO
cord-014597-66vd2mdu	1815	10	enhance	enhance	VB
cord-014597-66vd2mdu	1815	11	binding	bind	VBG
cord-014597-66vd2mdu	1815	12	affinity	affinity	NN
cord-014597-66vd2mdu	1815	13	by	by	IN
cord-014597-66vd2mdu	1815	14	three	three	CD
cord-014597-66vd2mdu	1815	15	new	new	JJ
cord-014597-66vd2mdu	1815	16	variants	variant	NNS
cord-014597-66vd2mdu	1815	17	with	with	IN
cord-014597-66vd2mdu	1815	18	backmutations	backmutation	NNS
cord-014597-66vd2mdu	1815	19	in	in	IN
cord-014597-66vd2mdu	1815	20	the	the	DT
cord-014597-66vd2mdu	1815	21	variable	variable	JJ
cord-014597-66vd2mdu	1815	22	light	light	JJ
cord-014597-66vd2mdu	1815	23	chain	chain	NN
cord-014597-66vd2mdu	1815	24	(	(	-LRB-
cord-014597-66vd2mdu	1815	25	VL	VL	NNP
cord-014597-66vd2mdu	1815	26	)	)	-RRB-
cord-014597-66vd2mdu	1815	27	.	.	.
cord-014597-66vd2mdu	1816	1	Originating	originate	VBG
cord-014597-66vd2mdu	1816	2	from	from	IN
cord-014597-66vd2mdu	1816	3	T98hR	t98hr	NN
cord-014597-66vd2mdu	1816	4	,	,	,
cord-014597-66vd2mdu	1816	5	residues	residue	NNS
cord-014597-66vd2mdu	1816	6	in	in	IN
cord-014597-66vd2mdu	1816	7	the	the	DT
cord-014597-66vd2mdu	1816	8	VL	VL	NNP
cord-014597-66vd2mdu	1816	9	were	be	VBD
cord-014597-66vd2mdu	1816	10	selected	select	VBN
cord-014597-66vd2mdu	1816	11	based	base	VBN
cord-014597-66vd2mdu	1816	12	on	on	IN
cord-014597-66vd2mdu	1816	13	their	-PRON-	PRP$
cord-014597-66vd2mdu	1816	14	spatial	spatial	JJ
cord-014597-66vd2mdu	1816	15	proximity	proximity	NN
cord-014597-66vd2mdu	1816	16	to	to	IN
cord-014597-66vd2mdu	1816	17	the	the	DT
cord-014597-66vd2mdu	1816	18	CDR3	CDR3	NNP
cord-014597-66vd2mdu	1816	19	loop	loop	NN
cord-014597-66vd2mdu	1816	20	of	of	IN
cord-014597-66vd2mdu	1816	21	the	the	DT
cord-014597-66vd2mdu	1816	22	variable	variable	JJ
cord-014597-66vd2mdu	1816	23	heavy	heavy	JJ
cord-014597-66vd2mdu	1816	24	chain	chain	NN
cord-014597-66vd2mdu	1816	25	.	.	.
cord-014597-66vd2mdu	1817	1	Affinity	affinity	NN
cord-014597-66vd2mdu	1817	2	improvement	improvement	NN
cord-014597-66vd2mdu	1817	3	of	of	IN
cord-014597-66vd2mdu	1817	4	T98hR	t98hr	NN
cord-014597-66vd2mdu	1817	5	was	be	VBD
cord-014597-66vd2mdu	1817	6	evaluated	evaluate	VBN
cord-014597-66vd2mdu	1817	7	by	by	IN
cord-014597-66vd2mdu	1817	8	VL	VL	NNP
cord-014597-66vd2mdu	1817	9	-	-	HYPH
cord-014597-66vd2mdu	1817	10	double	double	JJ
cord-014597-66vd2mdu	1817	11	backmutation	backmutation	NN
cord-014597-66vd2mdu	1817	12	variants	variant	NNS
cord-014597-66vd2mdu	1818	1	T98hR	t98hr	NN
cord-014597-66vd2mdu	1819	1	+	+	CC
cord-014597-66vd2mdu	1820	1	F46lL	F46lL	NNP
cord-014597-66vd2mdu	1820	2	(	(	-LRB-
cord-014597-66vd2mdu	1820	3	SU01	SU01	NNP
cord-014597-66vd2mdu	1820	4	)	)	-RRB-
cord-014597-66vd2mdu	1821	1	and	and	CC
cord-014597-66vd2mdu	1821	2	T98hR	t98hr	NN
cord-014597-66vd2mdu	1821	3	+	+	SYM
cord-014597-66vd2mdu	1821	4	Q49lS	Q49lS	NNP
cord-014597-66vd2mdu	1821	5	(	(	-LRB-
cord-014597-66vd2mdu	1821	6	SU02	SU02	NNP
cord-014597-66vd2mdu	1821	7	)	)	-RRB-
cord-014597-66vd2mdu	1821	8	and	and	CC
cord-014597-66vd2mdu	1821	9	a	a	DT
cord-014597-66vd2mdu	1821	10	triple	triple	JJ
cord-014597-66vd2mdu	1821	11	backmutation	backmutation	NN
cord-014597-66vd2mdu	1821	12	variant	variant	NN
cord-014597-66vd2mdu	1821	13	T98hR	t98hr	CD
cord-014597-66vd2mdu	1821	14	+	+	CC
cord-014597-66vd2mdu	1822	1	F46lL	F46lL	NNP
cord-014597-66vd2mdu	1823	1	+	+	CC
cord-014597-66vd2mdu	1823	2	Q49lS	Q49lS	NNP
cord-014597-66vd2mdu	1823	3	(	(	-LRB-
cord-014597-66vd2mdu	1823	4	SU03	SU03	NNP
cord-014597-66vd2mdu	1823	5	)	)	-RRB-
cord-014597-66vd2mdu	1823	6	.	.	.
cord-014597-66vd2mdu	1824	1	All	all	DT
cord-014597-66vd2mdu	1824	2	five	five	CD
cord-014597-66vd2mdu	1824	3	variants	variant	NNS
cord-014597-66vd2mdu	1824	4	were	be	VBD
cord-014597-66vd2mdu	1824	5	expressed	express	VBN
cord-014597-66vd2mdu	1824	6	transiently	transiently	RB
cord-014597-66vd2mdu	1824	7	in	in	IN
cord-014597-66vd2mdu	1824	8	HEK293	HEK293	NNP
cord-014597-66vd2mdu	1824	9	-	-	HYPH
cord-014597-66vd2mdu	1824	10	6E	6E	NNP
cord-014597-66vd2mdu	1824	11	cells	cell	NNS
cord-014597-66vd2mdu	1824	12	and	and	CC
cord-014597-66vd2mdu	1824	13	binding	bind	VBG
cord-014597-66vd2mdu	1824	14	affinities	affinity	NNS
cord-014597-66vd2mdu	1824	15	were	be	VBD
cord-014597-66vd2mdu	1824	16	investigated	investigate	VBN
cord-014597-66vd2mdu	1824	17	in	in	IN
cord-014597-66vd2mdu	1824	18	two	two	CD
cord-014597-66vd2mdu	1824	19	individual	individual	JJ
cord-014597-66vd2mdu	1824	20	settings	setting	NNS
cord-014597-66vd2mdu	1824	21	with	with	IN
cord-014597-66vd2mdu	1824	22	bio	bio	NN
cord-014597-66vd2mdu	1824	23	-	-	NN
cord-014597-66vd2mdu	1824	24	layer	layer	NN
cord-014597-66vd2mdu	1824	25	interferometry	interferometry	NN
cord-014597-66vd2mdu	1824	26	.	.	.
cord-014597-66vd2mdu	1825	1	In	in	IN
cord-014597-66vd2mdu	1825	2	the	the	DT
cord-014597-66vd2mdu	1825	3	first	first	JJ
cord-014597-66vd2mdu	1825	4	approach	approach	NN
cord-014597-66vd2mdu	1825	5	concentrated	concentrated	JJ
cord-014597-66vd2mdu	1825	6	cell	cell	NN
cord-014597-66vd2mdu	1825	7	culture	culture	NN
cord-014597-66vd2mdu	1825	8	supernatants	supernatant	NNS
cord-014597-66vd2mdu	1825	9	were	be	VBD
cord-014597-66vd2mdu	1825	10	directly	directly	RB
cord-014597-66vd2mdu	1825	11	applied	apply	VBN
cord-014597-66vd2mdu	1825	12	and	and	CC
cord-014597-66vd2mdu	1825	13	mAbs	mAbs	NNP
cord-014597-66vd2mdu	1825	14	were	be	VBD
cord-014597-66vd2mdu	1825	15	captured	capture	VBN
cord-014597-66vd2mdu	1825	16	on	on	IN
cord-014597-66vd2mdu	1825	17	protein	protein	NN
cord-014597-66vd2mdu	1825	18	A	a	DT
cord-014597-66vd2mdu	1825	19	tips	tip	NNS
cord-014597-66vd2mdu	1825	20	,	,	,
cord-014597-66vd2mdu	1825	21	blocked	block	VBN
cord-014597-66vd2mdu	1825	22	with	with	IN
cord-014597-66vd2mdu	1825	23	3D6scFv	3D6scFv	NNS
cord-014597-66vd2mdu	1825	24	-	-	HYPH
cord-014597-66vd2mdu	1825	25	Fc	Fc	NNP
cord-014597-66vd2mdu	1825	26	and	and	CC
cord-014597-66vd2mdu	1825	27	the	the	DT
cord-014597-66vd2mdu	1825	28	association	association	NN
cord-014597-66vd2mdu	1825	29	and	and	CC
cord-014597-66vd2mdu	1825	30	dissociation	dissociation	NN
cord-014597-66vd2mdu	1825	31	of	of	IN
cord-014597-66vd2mdu	1825	32	2F5	2f5	CD
cord-014597-66vd2mdu	1825	33	IgG	igg	NN
cord-014597-66vd2mdu	1825	34	was	be	VBD
cord-014597-66vd2mdu	1825	35	measured	measure	VBN
cord-014597-66vd2mdu	1825	36	.	.	.
cord-014597-66vd2mdu	1826	1	For	for	IN
cord-014597-66vd2mdu	1826	2	the	the	DT
cord-014597-66vd2mdu	1826	3	second	second	JJ
cord-014597-66vd2mdu	1826	4	approach	approach	NN
cord-014597-66vd2mdu	1826	5	,	,	,
cord-014597-66vd2mdu	1826	6	the	the	DT
cord-014597-66vd2mdu	1826	7	culture	culture	NN
cord-014597-66vd2mdu	1826	8	supernatants	supernatant	NNS
cord-014597-66vd2mdu	1826	9	were	be	VBD
cord-014597-66vd2mdu	1826	10	purified	purify	VBN
cord-014597-66vd2mdu	1826	11	and	and	CC
cord-014597-66vd2mdu	1826	12	the	the	DT
cord-014597-66vd2mdu	1826	13	affinity	affinity	NN
cord-014597-66vd2mdu	1826	14	was	be	VBD
cord-014597-66vd2mdu	1826	15	determined	determine	VBN
cord-014597-66vd2mdu	1826	16	with	with	IN
cord-014597-66vd2mdu	1826	17	streptavidin	streptavidin	JJ
cord-014597-66vd2mdu	1826	18	biosensors	biosensor	NNS
cord-014597-66vd2mdu	1826	19	.	.	.
cord-014597-66vd2mdu	1827	1	First	first	RB
cord-014597-66vd2mdu	1827	2	,	,	,
cord-014597-66vd2mdu	1827	3	biotinylated	biotinylate	VBD
cord-014597-66vd2mdu	1827	4	2F5	2f5	CD
cord-014597-66vd2mdu	1827	5	IgG	igg	NN
cord-014597-66vd2mdu	1827	6	was	be	VBD
cord-014597-66vd2mdu	1827	7	bound	bind	VBN
cord-014597-66vd2mdu	1827	8	and	and	CC
cord-014597-66vd2mdu	1827	9	then	then	RB
cord-014597-66vd2mdu	1827	10	the	the	DT
cord-014597-66vd2mdu	1827	11	association	association	NN
cord-014597-66vd2mdu	1827	12	/	/	SYM
cord-014597-66vd2mdu	1827	13	dissociation	dissociation	NN
cord-014597-66vd2mdu	1827	14	of	of	IN
cord-014597-66vd2mdu	1827	15	the	the	DT
cord-014597-66vd2mdu	1827	16	purified	purify	VBN
cord-014597-66vd2mdu	1827	17	3H6	3h6	CD
cord-014597-66vd2mdu	1827	18	variants	variant	NNS
cord-014597-66vd2mdu	1827	19	was	be	VBD
cord-014597-66vd2mdu	1827	20	measured	measure	VBN
cord-014597-66vd2mdu	1827	21	.	.	.
cord-014597-66vd2mdu	1828	1	Affinity	affinity	NN
cord-014597-66vd2mdu	1828	2	evaluation	evaluation	NN
cord-014597-66vd2mdu	1828	3	of	of	IN
cord-014597-66vd2mdu	1828	4	concentrated	concentrated	JJ
cord-014597-66vd2mdu	1828	5	culture	culture	NN
cord-014597-66vd2mdu	1828	6	supernatants	supernatant	NNS
cord-014597-66vd2mdu	1828	7	with	with	IN
cord-014597-66vd2mdu	1828	8	protein	protein	NN
cord-014597-66vd2mdu	1828	9	A	a	DT
cord-014597-66vd2mdu	1828	10	sensor	sensor	NN
cord-014597-66vd2mdu	1828	11	tips	tip	NNS
cord-014597-66vd2mdu	1828	12	showed	show	VBD
cord-014597-66vd2mdu	1828	13	a	a	DT
cord-014597-66vd2mdu	1828	14	decrease	decrease	NN
cord-014597-66vd2mdu	1828	15	of	of	IN
cord-014597-66vd2mdu	1828	16	binding	bind	VBG
cord-014597-66vd2mdu	1828	17	affinity	affinity	NN
cord-014597-66vd2mdu	1828	18	of	of	IN
cord-014597-66vd2mdu	1828	19	BM09	BM09	NNP
cord-014597-66vd2mdu	1828	20	and	and	CC
cord-014597-66vd2mdu	1828	21	a	a	DT
cord-014597-66vd2mdu	1828	22	loss	loss	NN
cord-014597-66vd2mdu	1828	23	of	of	IN
cord-014597-66vd2mdu	1828	24	binding	binding	NN
cord-014597-66vd2mdu	1828	25	of	of	IN
cord-014597-66vd2mdu	1828	26	BM10	BM10	NNP
cord-014597-66vd2mdu	1828	27	.	.	.
cord-014597-66vd2mdu	1829	1	The	the	DT
cord-014597-66vd2mdu	1829	2	protein	protein	NN
cord-014597-66vd2mdu	1829	3	A	a	DT
cord-014597-66vd2mdu	1829	4	measurement	measurement	NN
cord-014597-66vd2mdu	1829	5	showed	show	VBD
cord-014597-66vd2mdu	1829	6	an	an	DT
cord-014597-66vd2mdu	1829	7	increased	increase	VBN
cord-014597-66vd2mdu	1829	8	binding	binding	NN
cord-014597-66vd2mdu	1829	9	strength	strength	NN
cord-014597-66vd2mdu	1829	10	of	of	IN
cord-014597-66vd2mdu	1829	11	SU01	SU01	NNP
cord-014597-66vd2mdu	1829	12	,	,	,
cord-014597-66vd2mdu	1829	13	SU02	SU02	NNP
cord-014597-66vd2mdu	1829	14	and	and	CC
cord-014597-66vd2mdu	1829	15	SU03	SU03	NNP
cord-014597-66vd2mdu	1829	16	compared	compare	VBN
cord-014597-66vd2mdu	1829	17	to	to	IN
cord-014597-66vd2mdu	1829	18	su3H6	su3h6	CD
cord-014597-66vd2mdu	1829	19	and	and	CC
cord-014597-66vd2mdu	1829	20	BM07	BM07	NNP
cord-014597-66vd2mdu	1829	21	.	.	.
cord-014597-66vd2mdu	1830	1	SU01	SU01	NNP
cord-014597-66vd2mdu	1830	2	and	and	CC
cord-014597-66vd2mdu	1830	3	SU03	SU03	NNP
cord-014597-66vd2mdu	1830	4	result	result	VBP
cord-014597-66vd2mdu	1830	5	in	in	IN
cord-014597-66vd2mdu	1830	6	a	a	DT
cord-014597-66vd2mdu	1830	7	higher	high	JJR
cord-014597-66vd2mdu	1830	8	binding	bind	VBG
cord-014597-66vd2mdu	1830	9	affinity	affinity	NN
cord-014597-66vd2mdu	1830	10	compared	compare	VBN
cord-014597-66vd2mdu	1830	11	to	to	IN
cord-014597-66vd2mdu	1830	12	SU02	SU02	NNP
cord-014597-66vd2mdu	1830	13	.	.	.
cord-014597-66vd2mdu	1831	1	These	these	DT
cord-014597-66vd2mdu	1831	2	results	result	NNS
cord-014597-66vd2mdu	1831	3	can	can	MD
cord-014597-66vd2mdu	1831	4	be	be	VB
cord-014597-66vd2mdu	1831	5	confirmed	confirm	VBN
cord-014597-66vd2mdu	1831	6	with	with	IN
cord-014597-66vd2mdu	1831	7	purified	purify	VBN
cord-014597-66vd2mdu	1831	8	variants	variant	NNS
cord-014597-66vd2mdu	1831	9	by	by	IN
cord-014597-66vd2mdu	1831	10	the	the	DT
cord-014597-66vd2mdu	1831	11	streptavidin	streptavidin	NNP
cord-014597-66vd2mdu	1831	12	bio	bio	NNP
cord-014597-66vd2mdu	1831	13	-	-	HYPH
cord-014597-66vd2mdu	1831	14	assay	assay	NN
cord-014597-66vd2mdu	1831	15	(	(	-LRB-
cord-014597-66vd2mdu	1831	16	Fig	Fig	NNP
cord-014597-66vd2mdu	1831	17	.	.	.
cord-014597-66vd2mdu	1831	18	1	1	CD
cord-014597-66vd2mdu	1831	19	)	)	-RRB-
cord-014597-66vd2mdu	1831	20	.	.	.
cord-014597-66vd2mdu	1832	1	Alanine	alanine	NN
cord-014597-66vd2mdu	1832	2	scanning	scanning	NN
cord-014597-66vd2mdu	1832	3	of	of	IN
cord-014597-66vd2mdu	1832	4	the	the	DT
cord-014597-66vd2mdu	1832	5	tyrosine	tyrosine	NN
cord-014597-66vd2mdu	1832	6	cage	cage	NN
cord-014597-66vd2mdu	1832	7	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	1832	8	a	a	DT
cord-014597-66vd2mdu	1832	9	reduction	reduction	NN
cord-014597-66vd2mdu	1832	10	of	of	IN
cord-014597-66vd2mdu	1832	11	binding	bind	VBG
cord-014597-66vd2mdu	1832	12	affinity	affinity	NN
cord-014597-66vd2mdu	1832	13	(	(	-LRB-
cord-014597-66vd2mdu	1832	14	BM09	BM09	NNP
cord-014597-66vd2mdu	1832	15	)	)	-RRB-
cord-014597-66vd2mdu	1832	16	and	and	CC
cord-014597-66vd2mdu	1832	17	a	a	DT
cord-014597-66vd2mdu	1832	18	severe	severe	JJ
cord-014597-66vd2mdu	1832	19	loss	loss	NN
cord-014597-66vd2mdu	1832	20	of	of	IN
cord-014597-66vd2mdu	1832	21	binding	bind	VBG
cord-014597-66vd2mdu	1832	22	(	(	-LRB-
cord-014597-66vd2mdu	1832	23	BM10	BM10	NNP
cord-014597-66vd2mdu	1832	24	)	)	-RRB-
cord-014597-66vd2mdu	1832	25	,	,	,
cord-014597-66vd2mdu	1832	26	concluding	conclude	VBG
cord-014597-66vd2mdu	1832	27	that	that	IN
cord-014597-66vd2mdu	1832	28	the	the	DT
cord-014597-66vd2mdu	1832	29	tyrosine	tyrosine	NN
cord-014597-66vd2mdu	1832	30	cage	cage	NN
cord-014597-66vd2mdu	1832	31	plays	play	VBZ
cord-014597-66vd2mdu	1832	32	an	an	DT
cord-014597-66vd2mdu	1832	33	important	important	JJ
cord-014597-66vd2mdu	1832	34	role	role	NN
cord-014597-66vd2mdu	1832	35	for	for	IN
cord-014597-66vd2mdu	1832	36	supporting	support	VBG
cord-014597-66vd2mdu	1832	37	a	a	DT
cord-014597-66vd2mdu	1832	38	correct	correct	JJ
cord-014597-66vd2mdu	1832	39	CDR	cdr	NN
cord-014597-66vd2mdu	1832	40	loop	loop	NN
cord-014597-66vd2mdu	1832	41	conformation	conformation	NN
cord-014597-66vd2mdu	1832	42	.	.	.
cord-014597-66vd2mdu	1833	1	Further	further	JJ
cord-014597-66vd2mdu	1833	2	affinity	affinity	NN
cord-014597-66vd2mdu	1833	3	improvement	improvement	NN
cord-014597-66vd2mdu	1833	4	of	of	IN
cord-014597-66vd2mdu	1833	5	the	the	DT
cord-014597-66vd2mdu	1833	6	single	single	JJ
cord-014597-66vd2mdu	1833	7	mutation	mutation	NN
cord-014597-66vd2mdu	1833	8	variant	variant	NN
cord-014597-66vd2mdu	1833	9	T98hR	t98hr	PRP
cord-014597-66vd2mdu	1833	10	could	could	MD
cord-014597-66vd2mdu	1833	11	have	have	VB
cord-014597-66vd2mdu	1833	12	been	be	VBN
cord-014597-66vd2mdu	1833	13	reached	reach	VBN
cord-014597-66vd2mdu	1833	14	via	via	IN
cord-014597-66vd2mdu	1833	15	mutations	mutation	NNS
cord-014597-66vd2mdu	1833	16	in	in	IN
cord-014597-66vd2mdu	1833	17	the	the	DT
cord-014597-66vd2mdu	1833	18	VL	VL	NNP
cord-014597-66vd2mdu	1833	19	.	.	.
cord-014597-66vd2mdu	1834	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1834	2	demonstrates	demonstrate	VBZ
cord-014597-66vd2mdu	1834	3	the	the	DT
cord-014597-66vd2mdu	1834	4	underestimated	underestimated	JJ
cord-014597-66vd2mdu	1834	5	role	role	NN
cord-014597-66vd2mdu	1834	6	of	of	IN
cord-014597-66vd2mdu	1834	7	the	the	DT
cord-014597-66vd2mdu	1834	8	VL	VL	NNP
cord-014597-66vd2mdu	1834	9	for	for	IN
cord-014597-66vd2mdu	1834	10	the	the	DT
cord-014597-66vd2mdu	1834	11	interaction	interaction	NN
cord-014597-66vd2mdu	1834	12	with	with	IN
cord-014597-66vd2mdu	1834	13	its	-PRON-	PRP$
cord-014597-66vd2mdu	1834	14	binding	bind	VBG
cord-014597-66vd2mdu	1834	15	partner	partner	NN
cord-014597-66vd2mdu	1834	16	.	.	.
cord-014597-66vd2mdu	1835	1	Although	although	IN
cord-014597-66vd2mdu	1835	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1835	3	cells	cell	NNS
cord-014597-66vd2mdu	1835	4	are	be	VBP
cord-014597-66vd2mdu	1835	5	a	a	DT
cord-014597-66vd2mdu	1835	6	major	major	JJ
cord-014597-66vd2mdu	1835	7	expression	expression	NN
cord-014597-66vd2mdu	1835	8	system	system	NN
cord-014597-66vd2mdu	1835	9	for	for	IN
cord-014597-66vd2mdu	1835	10	production	production	NN
cord-014597-66vd2mdu	1835	11	of	of	IN
cord-014597-66vd2mdu	1835	12	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1835	13	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	1835	14	,	,	,
cord-014597-66vd2mdu	1835	15	the	the	DT
cord-014597-66vd2mdu	1835	16	molecular	molecular	JJ
cord-014597-66vd2mdu	1835	17	and	and	CC
cord-014597-66vd2mdu	1835	18	cellular	cellular	JJ
cord-014597-66vd2mdu	1835	19	background	background	NN
cord-014597-66vd2mdu	1835	20	characterizing	characterize	VBG
cord-014597-66vd2mdu	1835	21	a	a	DT
cord-014597-66vd2mdu	1835	22	high	high	JJ
cord-014597-66vd2mdu	1835	23	producer	producer	NN
cord-014597-66vd2mdu	1835	24	is	be	VBZ
cord-014597-66vd2mdu	1835	25	largely	largely	RB
cord-014597-66vd2mdu	1835	26	unknown	unknown	JJ
cord-014597-66vd2mdu	1835	27	.	.	.
cord-014597-66vd2mdu	1836	1	It	-PRON-	PRP
cord-014597-66vd2mdu	1836	2	has	have	VBZ
cord-014597-66vd2mdu	1836	3	been	be	VBN
cord-014597-66vd2mdu	1836	4	observed	observe	VBN
cord-014597-66vd2mdu	1836	5	that	that	IN
cord-014597-66vd2mdu	1836	6	in	in	IN
cord-014597-66vd2mdu	1836	7	producer	producer	NN
cord-014597-66vd2mdu	1836	8	cell	cell	NN
cord-014597-66vd2mdu	1836	9	lines	line	NNS
cord-014597-66vd2mdu	1836	10	important	important	JJ
cord-014597-66vd2mdu	1836	11	signaling	signal	VBG
cord-014597-66vd2mdu	1836	12	pathways	pathway	NNS
cord-014597-66vd2mdu	1836	13	like	like	IN
cord-014597-66vd2mdu	1836	14	the	the	DT
cord-014597-66vd2mdu	1836	15	Akt	Akt	NNP
cord-014597-66vd2mdu	1836	16	-	-	HYPH
cord-014597-66vd2mdu	1836	17	signaling	signaling	NN
cord-014597-66vd2mdu	1836	18	are	be	VBP
cord-014597-66vd2mdu	1836	19	altered	alter	VBN
cord-014597-66vd2mdu	1836	20	in	in	IN
cord-014597-66vd2mdu	1836	21	characteristical	characteristical	JJ
cord-014597-66vd2mdu	1836	22	ways	way	NNS
cord-014597-66vd2mdu	1836	23	.	.	.
cord-014597-66vd2mdu	1837	1	Thus	thus	RB
cord-014597-66vd2mdu	1837	2	analyzing	analyze	VBG
cord-014597-66vd2mdu	1837	3	according	accord	VBG
cord-014597-66vd2mdu	1837	4	signaling	signal	VBG
cord-014597-66vd2mdu	1837	5	events	event	NNS
cord-014597-66vd2mdu	1837	6	should	should	MD
cord-014597-66vd2mdu	1837	7	lead	lead	VB
cord-014597-66vd2mdu	1837	8	to	to	IN
cord-014597-66vd2mdu	1837	9	identification	identification	NN
cord-014597-66vd2mdu	1837	10	of	of	IN
cord-014597-66vd2mdu	1837	11	key	key	JJ
cord-014597-66vd2mdu	1837	12	elements	element	NNS
cord-014597-66vd2mdu	1837	13	characterizing	characterize	VBG
cord-014597-66vd2mdu	1837	14	high	high	JJ
cord-014597-66vd2mdu	1837	15	producer	producer	NN
cord-014597-66vd2mdu	1837	16	cells	cell	NNS
cord-014597-66vd2mdu	1837	17	.	.	.
cord-014597-66vd2mdu	1838	1	To	to	TO
cord-014597-66vd2mdu	1838	2	investigate	investigate	VB
cord-014597-66vd2mdu	1838	3	this	this	DT
cord-014597-66vd2mdu	1838	4	,	,	,
cord-014597-66vd2mdu	1838	5	our	-PRON-	PRP$
cord-014597-66vd2mdu	1838	6	emphasis	emphasis	NN
cord-014597-66vd2mdu	1838	7	lies	lie	VBZ
cord-014597-66vd2mdu	1838	8	on	on	IN
cord-014597-66vd2mdu	1838	9	the	the	DT
cord-014597-66vd2mdu	1838	10	phosphorylation	phosphorylation	NN
cord-014597-66vd2mdu	1838	11	status	status	NN
cord-014597-66vd2mdu	1838	12	of	of	IN
cord-014597-66vd2mdu	1838	13	involved	involved	JJ
cord-014597-66vd2mdu	1838	14	proteins	protein	NNS
cord-014597-66vd2mdu	1838	15	as	as	IN
cord-014597-66vd2mdu	1838	16	reversible	reversible	JJ
cord-014597-66vd2mdu	1838	17	switches	switch	NNS
cord-014597-66vd2mdu	1838	18	in	in	IN
cord-014597-66vd2mdu	1838	19	all	all	DT
cord-014597-66vd2mdu	1838	20	signaling	signal	VBG
cord-014597-66vd2mdu	1838	21	pathways	pathway	NNS
cord-014597-66vd2mdu	1838	22	.	.	.
cord-014597-66vd2mdu	1839	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1839	2	aimed	aim	VBD
cord-014597-66vd2mdu	1839	3	to	to	TO
cord-014597-66vd2mdu	1839	4	establish	establish	VB
cord-014597-66vd2mdu	1839	5	a	a	DT
cord-014597-66vd2mdu	1839	6	workflow	workflow	NN
cord-014597-66vd2mdu	1839	7	for	for	IN
cord-014597-66vd2mdu	1839	8	CHO	CHO	NNP
cord-014597-66vd2mdu	1839	9	-	-	HYPH
cord-014597-66vd2mdu	1839	10	specific	specific	JJ
cord-014597-66vd2mdu	1839	11	phosphoproteomics	phosphoproteomic	NNS
cord-014597-66vd2mdu	1839	12	and	and	CC
cord-014597-66vd2mdu	1839	13	focused	focus	VBD
cord-014597-66vd2mdu	1839	14	on	on	IN
cord-014597-66vd2mdu	1839	15	IGF	IGF	NNP
cord-014597-66vd2mdu	1839	16	signaling	signaling	NN
cord-014597-66vd2mdu	1839	17	,	,	,
cord-014597-66vd2mdu	1839	18	as	as	IN
cord-014597-66vd2mdu	1839	19	cell	cell	NN
cord-014597-66vd2mdu	1839	20	culture	culture	NN
cord-014597-66vd2mdu	1839	21	media	medium	NNS
cord-014597-66vd2mdu	1839	22	often	often	RB
cord-014597-66vd2mdu	1839	23	are	be	VBP
cord-014597-66vd2mdu	1839	24	supplemented	supplement	VBN
cord-014597-66vd2mdu	1839	25	with	with	IN
cord-014597-66vd2mdu	1839	26	this	this	DT
cord-014597-66vd2mdu	1839	27	growth	growth	NN
cord-014597-66vd2mdu	1839	28	factor	factor	NN
cord-014597-66vd2mdu	1839	29	.	.	.
cord-014597-66vd2mdu	1840	1	Two	two	CD
cord-014597-66vd2mdu	1840	2	producer	producer	NN
cord-014597-66vd2mdu	1840	3	cell	cell	NN
cord-014597-66vd2mdu	1840	4	lines	line	NNS
cord-014597-66vd2mdu	1840	5	and	and	CC
cord-014597-66vd2mdu	1840	6	the	the	DT
cord-014597-66vd2mdu	1840	7	according	accord	VBG
cord-014597-66vd2mdu	1840	8	parental	parental	JJ
cord-014597-66vd2mdu	1840	9	cells	cell	NNS
cord-014597-66vd2mdu	1840	10	were	be	VBD
cord-014597-66vd2mdu	1840	11	cultivated	cultivate	VBN
cord-014597-66vd2mdu	1840	12	in	in	IN
cord-014597-66vd2mdu	1840	13	a	a	DT
cord-014597-66vd2mdu	1840	14	stable	stable	JJ
cord-014597-66vd2mdu	1840	15	isotope	isotope	NN
cord-014597-66vd2mdu	1840	16	labeling	labeling	NN
cord-014597-66vd2mdu	1840	17	with	with	IN
cord-014597-66vd2mdu	1840	18	amino	amino	JJ
cord-014597-66vd2mdu	1840	19	acids	acid	NNS
cord-014597-66vd2mdu	1840	20	in	in	IN
cord-014597-66vd2mdu	1840	21	cell	cell	NN
cord-014597-66vd2mdu	1840	22	culture	culture	NN
cord-014597-66vd2mdu	1840	23	(	(	-LRB-
cord-014597-66vd2mdu	1840	24	SILAC	SILAC	NNP
cord-014597-66vd2mdu	1840	25	)	)	-RRB-
cord-014597-66vd2mdu	1840	26	experiment	experiment	NN
cord-014597-66vd2mdu	1840	27	,	,	,
cord-014597-66vd2mdu	1840	28	followed	follow	VBN
cord-014597-66vd2mdu	1840	29	by	by	IN
cord-014597-66vd2mdu	1840	30	quantitative	quantitative	JJ
cord-014597-66vd2mdu	1840	31	MS	MS	NNP
cord-014597-66vd2mdu	1840	32	phosphoproteomic	phosphoproteomic	NNP
cord-014597-66vd2mdu	1840	33	analysis	analysis	NN
cord-014597-66vd2mdu	1840	34	including	include	VBG
cord-014597-66vd2mdu	1840	35	CHOspecific	CHOspecific	NNP
cord-014597-66vd2mdu	1840	36	data	data	NN
cord-014597-66vd2mdu	1840	37	evaluation	evaluation	NN
cord-014597-66vd2mdu	1840	38	.	.	.
cord-014597-66vd2mdu	1841	1	The	the	DT
cord-014597-66vd2mdu	1841	2	chosen	choose	VBN
cord-014597-66vd2mdu	1841	3	CHO	CHO	NNP
cord-014597-66vd2mdu	1841	4	cell	cell	NN
cord-014597-66vd2mdu	1841	5	lines	line	NNS
cord-014597-66vd2mdu	1841	6	were	be	VBD
cord-014597-66vd2mdu	1841	7	cultivated	cultivate	VBN
cord-014597-66vd2mdu	1841	8	in	in	IN
cord-014597-66vd2mdu	1841	9	triplicates	triplicate	NNS
cord-014597-66vd2mdu	1841	10	in	in	IN
cord-014597-66vd2mdu	1841	11	SILAC	SILAC	NNP
cord-014597-66vd2mdu	1841	12	media	medium	NNS
cord-014597-66vd2mdu	1841	13	containing	contain	VBG
cord-014597-66vd2mdu	1841	14	isotopically	isotopically	RB
cord-014597-66vd2mdu	1841	15	-	-	HYPH
cord-014597-66vd2mdu	1841	16	labeled	label	VBN
cord-014597-66vd2mdu	1841	17	lysine	lysine	NN
cord-014597-66vd2mdu	1841	18	/	/	SYM
cord-014597-66vd2mdu	1841	19	arginine	arginine	NN
cord-014597-66vd2mdu	1841	20	(	(	-LRB-
cord-014597-66vd2mdu	1841	21	hLys	hLys	NNP
cord-014597-66vd2mdu	1841	22	/	/	SYM
cord-014597-66vd2mdu	1841	23	hArg	hArg	NNP
cord-014597-66vd2mdu	1841	24	)	)	-RRB-
cord-014597-66vd2mdu	1841	25	and	and	CC
cord-014597-66vd2mdu	1841	26	in	in	IN
cord-014597-66vd2mdu	1841	27	parallel	parallel	NN
cord-014597-66vd2mdu	1841	28	in	in	IN
cord-014597-66vd2mdu	1841	29	identical	identical	JJ
cord-014597-66vd2mdu	1841	30	standard	standard	JJ
cord-014597-66vd2mdu	1841	31	media	medium	NNS
cord-014597-66vd2mdu	1841	32	(	(	-LRB-
cord-014597-66vd2mdu	1841	33	lLys	lLys	NNP
cord-014597-66vd2mdu	1841	34	/	/	SYM
cord-014597-66vd2mdu	1841	35	lArg	lArg	NNP
cord-014597-66vd2mdu	1841	36	,	,	,
cord-014597-66vd2mdu	1841	37	TCX10D	tcx10d	NN
cord-014597-66vd2mdu	1841	38	,	,	,
cord-014597-66vd2mdu	1841	39	Xell	Xell	NNP
cord-014597-66vd2mdu	1841	40	)	)	-RRB-
cord-014597-66vd2mdu	1841	41	.	.	.
cord-014597-66vd2mdu	1842	1	Cell	cell	NN
cord-014597-66vd2mdu	1842	2	density	density	NN
cord-014597-66vd2mdu	1842	3	,	,	,
cord-014597-66vd2mdu	1842	4	viability	viability	NN
cord-014597-66vd2mdu	1842	5	,	,	,
cord-014597-66vd2mdu	1842	6	metabolism	metabolism	NN
cord-014597-66vd2mdu	1842	7	and	and	CC
cord-014597-66vd2mdu	1842	8	cell	cell	NN
cord-014597-66vd2mdu	1842	9	cycle	cycle	NN
cord-014597-66vd2mdu	1842	10	distribution	distribution	NN
cord-014597-66vd2mdu	1842	11	were	be	VBD
cord-014597-66vd2mdu	1842	12	monitored	monitor	VBN
cord-014597-66vd2mdu	1842	13	during	during	IN
cord-014597-66vd2mdu	1842	14	50	50	CD
cord-014597-66vd2mdu	1842	15	ml	ml	NN
cord-014597-66vd2mdu	1842	16	batch	batch	NN
cord-014597-66vd2mdu	1842	17	culture	culture	NN
cord-014597-66vd2mdu	1842	18	for	for	IN
cord-014597-66vd2mdu	1842	19	7	7	CD
cord-014597-66vd2mdu	1842	20	-	-	SYM
cord-014597-66vd2mdu	1842	21	8	8	CD
cord-014597-66vd2mdu	1842	22	days	day	NNS
cord-014597-66vd2mdu	1842	23	.	.	.
cord-014597-66vd2mdu	1843	1	At	at	IN
cord-014597-66vd2mdu	1843	2	day	day	NN
cord-014597-66vd2mdu	1843	3	3.25	3.25	CD
cord-014597-66vd2mdu	1843	4	IGF	IGF	NNP
cord-014597-66vd2mdu	1843	5	was	be	VBD
cord-014597-66vd2mdu	1843	6	added	add	VBN
cord-014597-66vd2mdu	1843	7	into	into	IN
cord-014597-66vd2mdu	1843	8	hLys	hLys	NNP
cord-014597-66vd2mdu	1843	9	/	/	SYM
cord-014597-66vd2mdu	1843	10	hArg	hArg	NNP
cord-014597-66vd2mdu	1843	11	cultures	culture	NNS
cord-014597-66vd2mdu	1843	12	.	.	.
cord-014597-66vd2mdu	1844	1	5	5	CD
cord-014597-66vd2mdu	1844	2	min	min	NNS
cord-014597-66vd2mdu	1844	3	later	later	RB
cord-014597-66vd2mdu	1844	4	a	a	DT
cord-014597-66vd2mdu	1844	5	part	part	NN
cord-014597-66vd2mdu	1844	6	of	of	IN
cord-014597-66vd2mdu	1844	7	the	the	DT
cord-014597-66vd2mdu	1844	8	cells	cell	NNS
cord-014597-66vd2mdu	1844	9	was	be	VBD
cord-014597-66vd2mdu	1844	10	harvested	harvest	VBN
cord-014597-66vd2mdu	1844	11	.	.	.
cord-014597-66vd2mdu	1845	1	For	for	IN
cord-014597-66vd2mdu	1845	2	MS	MS	NNP
cord-014597-66vd2mdu	1845	3	analysis	analysis	NN
cord-014597-66vd2mdu	1845	4	IGF	IGF	NNP
cord-014597-66vd2mdu	1845	5	-	-	HYPH
cord-014597-66vd2mdu	1845	6	treated	treat	VBN
cord-014597-66vd2mdu	1845	7	(	(	-LRB-
cord-014597-66vd2mdu	1845	8	hLys	hLys	NNP
cord-014597-66vd2mdu	1845	9	/	/	SYM
cord-014597-66vd2mdu	1845	10	hArg	hArg	NNP
cord-014597-66vd2mdu	1845	11	cultures	culture	NNS
cord-014597-66vd2mdu	1845	12	)	)	-RRB-
cord-014597-66vd2mdu	1845	13	and	and	CC
cord-014597-66vd2mdu	1845	14	control	control	NN
cord-014597-66vd2mdu	1845	15	cultures	culture	NNS
cord-014597-66vd2mdu	1845	16	(	(	-LRB-
cord-014597-66vd2mdu	1845	17	lLys	lLys	NNP
cord-014597-66vd2mdu	1845	18	/	/	SYM
cord-014597-66vd2mdu	1845	19	lArg	lArg	NNP
cord-014597-66vd2mdu	1845	20	cultures	culture	NNS
cord-014597-66vd2mdu	1845	21	)	)	-RRB-
cord-014597-66vd2mdu	1845	22	were	be	VBD
cord-014597-66vd2mdu	1845	23	combined	combine	VBN
cord-014597-66vd2mdu	1845	24	.	.	.
cord-014597-66vd2mdu	1846	1	The	the	DT
cord-014597-66vd2mdu	1846	2	following	follow	VBG
cord-014597-66vd2mdu	1846	3	MS	MS	NNP
cord-014597-66vd2mdu	1846	4	sample	sample	NN
cord-014597-66vd2mdu	1846	5	preparation	preparation	NN
cord-014597-66vd2mdu	1846	6	workflow	workflow	NN
cord-014597-66vd2mdu	1846	7	included	include	VBD
cord-014597-66vd2mdu	1846	8	digestion	digestion	NN
cord-014597-66vd2mdu	1846	9	of	of	IN
cord-014597-66vd2mdu	1846	10	whole	whole	JJ
cord-014597-66vd2mdu	1846	11	protein	protein	NN
cord-014597-66vd2mdu	1846	12	lysate	lysate	NN
cord-014597-66vd2mdu	1846	13	and	and	CC
cord-014597-66vd2mdu	1846	14	phosphopeptide	phosphopeptide	NN
cord-014597-66vd2mdu	1846	15	enrichment	enrichment	NN
cord-014597-66vd2mdu	1846	16	via	via	IN
cord-014597-66vd2mdu	1846	17	TIO	TIO	NNP
cord-014597-66vd2mdu	1846	18	2beads	2beads	CD
cord-014597-66vd2mdu	1846	19	.	.	.
cord-014597-66vd2mdu	1847	1	NanoLC	NanoLC	NNP
cord-014597-66vd2mdu	1847	2	-	-	HYPH
cord-014597-66vd2mdu	1847	3	ESI	ESI	NNP
cord-014597-66vd2mdu	1848	1	-Orbitrap	-orbitrap	IN
cord-014597-66vd2mdu	1848	2	MS	MS	NNP
cord-014597-66vd2mdu	1848	3	(	(	-LRB-
cord-014597-66vd2mdu	1848	4	Q	Q	NNP
cord-014597-66vd2mdu	1848	5	Exactive	Exactive	NNP
cord-014597-66vd2mdu	1848	6	Plus	Plus	NNP
cord-014597-66vd2mdu	1848	7	,	,	,
cord-014597-66vd2mdu	1848	8	Thermo	Thermo	NNP
cord-014597-66vd2mdu	1848	9	Fisher	Fisher	NNP
cord-014597-66vd2mdu	1848	10	Scientific	Scientific	NNP
cord-014597-66vd2mdu	1848	11	)	)	-RRB-
cord-014597-66vd2mdu	1848	12	of	of	IN
cord-014597-66vd2mdu	1848	13	phosphopeptides	phosphopeptides	NNP
cord-014597-66vd2mdu	1848	14	was	be	VBD
cord-014597-66vd2mdu	1848	15	excecuted	excecute	VBN
cord-014597-66vd2mdu	1848	16	with	with	IN
cord-014597-66vd2mdu	1848	17	subsequent	subsequent	JJ
cord-014597-66vd2mdu	1848	18	identification	identification	NN
cord-014597-66vd2mdu	1848	19	and	and	CC
cord-014597-66vd2mdu	1848	20	quantification	quantification	NN
cord-014597-66vd2mdu	1848	21	in	in	IN
cord-014597-66vd2mdu	1848	22	MaxQuant	MaxQuant	NNP
cord-014597-66vd2mdu	1848	23	[	[	-LRB-
cord-014597-66vd2mdu	1848	24	1	1	CD
cord-014597-66vd2mdu	1848	25	]	]	-RRB-
cord-014597-66vd2mdu	1848	26	.	.	.
cord-014597-66vd2mdu	1849	1	In	in	IN
cord-014597-66vd2mdu	1849	2	addition	addition	NN
cord-014597-66vd2mdu	1849	3	to	to	IN
cord-014597-66vd2mdu	1849	4	SILAC	SILAC	NNP
cord-014597-66vd2mdu	1849	5	quantification	quantification	NN
cord-014597-66vd2mdu	1849	6	of	of	IN
cord-014597-66vd2mdu	1849	7	H	H	NNP
cord-014597-66vd2mdu	1849	8	/	/	SYM
cord-014597-66vd2mdu	1849	9	L	L	NNP
cord-014597-66vd2mdu	1849	10	ratios	ratio	NNS
cord-014597-66vd2mdu	1849	11	for	for	IN
cord-014597-66vd2mdu	1849	12	investigation	investigation	NN
cord-014597-66vd2mdu	1849	13	of	of	IN
cord-014597-66vd2mdu	1849	14	IGF	IGF	NNP
cord-014597-66vd2mdu	1849	15	effects	effect	NNS
cord-014597-66vd2mdu	1849	16	,	,	,
cord-014597-66vd2mdu	1849	17	aquired	aquire	VBN
cord-014597-66vd2mdu	1849	18	data	datum	NNS
cord-014597-66vd2mdu	1849	19	was	be	VBD
cord-014597-66vd2mdu	1849	20	also	also	RB
cord-014597-66vd2mdu	1849	21	used	use	VBN
cord-014597-66vd2mdu	1849	22	to	to	TO
cord-014597-66vd2mdu	1849	23	perform	perform	VB
cord-014597-66vd2mdu	1849	24	label	label	NN
cord-014597-66vd2mdu	1849	25	-	-	HYPH
cord-014597-66vd2mdu	1849	26	free	free	JJ
cord-014597-66vd2mdu	1849	27	quantification	quantification	NN
cord-014597-66vd2mdu	1849	28	(	(	-LRB-
cord-014597-66vd2mdu	1849	29	LFQ	LFQ	NNP
cord-014597-66vd2mdu	1849	30	)	)	-RRB-
cord-014597-66vd2mdu	1849	31	in	in	IN
cord-014597-66vd2mdu	1849	32	MaxQuant	MaxQuant	NNP
cord-014597-66vd2mdu	1849	33	[	[	-LRB-
cord-014597-66vd2mdu	1849	34	1	1	CD
cord-014597-66vd2mdu	1849	35	]	]	-RRB-
cord-014597-66vd2mdu	1850	1	for	for	IN
cord-014597-66vd2mdu	1850	2	comparison	comparison	NN
cord-014597-66vd2mdu	1850	3	of	of	IN
cord-014597-66vd2mdu	1850	4	cell	cell	NN
cord-014597-66vd2mdu	1850	5	lines	line	NNS
cord-014597-66vd2mdu	1850	6	.	.	.
cord-014597-66vd2mdu	1851	1	Statistical	statistical	JJ
cord-014597-66vd2mdu	1851	2	significance	significance	NN
cord-014597-66vd2mdu	1851	3	was	be	VBD
cord-014597-66vd2mdu	1851	4	calculated	calculate	VBN
cord-014597-66vd2mdu	1851	5	via	via	IN
cord-014597-66vd2mdu	1851	6	t	t	NN
cord-014597-66vd2mdu	1851	7	-	-	HYPH
cord-014597-66vd2mdu	1851	8	test	test	NN
cord-014597-66vd2mdu	1851	9	(	(	-LRB-
cord-014597-66vd2mdu	1851	10	p<0.05	p<0.05	NNP
cord-014597-66vd2mdu	1851	11	)	)	-RRB-
cord-014597-66vd2mdu	1851	12	or	or	CC
cord-014597-66vd2mdu	1851	13	ANOVA	ANOVA	NNP
cord-014597-66vd2mdu	1851	14	(	(	-LRB-
cord-014597-66vd2mdu	1851	15	permutation	permutation	NN
cord-014597-66vd2mdu	1851	16	-	-	HYPH
cord-014597-66vd2mdu	1851	17	based	base	VBN
cord-014597-66vd2mdu	1851	18	FDR<0.05	fdr<0.05	CD
cord-014597-66vd2mdu	1851	19	)	)	-RRB-
cord-014597-66vd2mdu	1851	20	in	in	IN
cord-014597-66vd2mdu	1851	21	Perseus	Perseus	NNP
cord-014597-66vd2mdu	1851	22	[	[	-LRB-
cord-014597-66vd2mdu	1851	23	2	2	CD
cord-014597-66vd2mdu	1851	24	]	]	-RRB-
cord-014597-66vd2mdu	1851	25	.	.	.
cord-014597-66vd2mdu	1852	1	Results	result	VBZ
cord-014597-66vd2mdu	1852	2	IGF	IGF	NNP
cord-014597-66vd2mdu	1852	3	effects	effect	NNS
cord-014597-66vd2mdu	1852	4	on	on	IN
cord-014597-66vd2mdu	1852	5	growth	growth	NN
cord-014597-66vd2mdu	1852	6	and	and	CC
cord-014597-66vd2mdu	1852	7	production	production	NN
cord-014597-66vd2mdu	1853	1	The	the	DT
cord-014597-66vd2mdu	1853	2	IGF	IGF	NNP
cord-014597-66vd2mdu	1853	3	treatment	treatment	NN
cord-014597-66vd2mdu	1853	4	resulted	result	VBD
cord-014597-66vd2mdu	1853	5	in	in	IN
cord-014597-66vd2mdu	1853	6	a	a	DT
cord-014597-66vd2mdu	1853	7	prolonged	prolonged	JJ
cord-014597-66vd2mdu	1853	8	viability	viability	NN
cord-014597-66vd2mdu	1853	9	for	for	IN
cord-014597-66vd2mdu	1853	10	all	all	DT
cord-014597-66vd2mdu	1853	11	cell	cell	NN
cord-014597-66vd2mdu	1853	12	lines	line	NNS
cord-014597-66vd2mdu	1853	13	.	.	.
cord-014597-66vd2mdu	1854	1	However	however	RB
cord-014597-66vd2mdu	1854	2	,	,	,
cord-014597-66vd2mdu	1854	3	an	an	DT
cord-014597-66vd2mdu	1854	4	increased	increase	VBN
cord-014597-66vd2mdu	1854	5	VCD	vcd	NN
cord-014597-66vd2mdu	1854	6	was	be	VBD
cord-014597-66vd2mdu	1854	7	only	only	RB
cord-014597-66vd2mdu	1854	8	observed	observe	VBN
cord-014597-66vd2mdu	1854	9	for	for	IN
cord-014597-66vd2mdu	1854	10	producer	producer	NN
cord-014597-66vd2mdu	1854	11	cell	cell	NN
cord-014597-66vd2mdu	1854	12	line	line	NN
cord-014597-66vd2mdu	1854	13	1	1	CD
cord-014597-66vd2mdu	1854	14	,	,	,
cord-014597-66vd2mdu	1854	15	yielding	yield	VBG
cord-014597-66vd2mdu	1854	16	in	in	IN
cord-014597-66vd2mdu	1854	17	an	an	DT
cord-014597-66vd2mdu	1854	18	enhanced	enhance	VBN
cord-014597-66vd2mdu	1854	19	integral	integral	NN
cord-014597-66vd2mdu	1854	20	of	of	IN
cord-014597-66vd2mdu	1854	21	VCD	vcd	NN
cord-014597-66vd2mdu	1854	22	(	(	-LRB-
cord-014597-66vd2mdu	1854	23	IVCD	IVCD	NNP
cord-014597-66vd2mdu	1854	24	)	)	-RRB-
cord-014597-66vd2mdu	1854	25	.	.	.
cord-014597-66vd2mdu	1855	1	For	for	IN
cord-014597-66vd2mdu	1855	2	the	the	DT
cord-014597-66vd2mdu	1855	3	parental	parental	JJ
cord-014597-66vd2mdu	1855	4	cells	cell	NNS
cord-014597-66vd2mdu	1855	5	growth	growth	NN
cord-014597-66vd2mdu	1855	6	was	be	VBD
cord-014597-66vd2mdu	1855	7	inhibited	inhibit	VBN
cord-014597-66vd2mdu	1855	8	by	by	IN
cord-014597-66vd2mdu	1855	9	IGF	IGF	NNP
cord-014597-66vd2mdu	1855	10	,	,	,
cord-014597-66vd2mdu	1855	11	although	although	IN
cord-014597-66vd2mdu	1855	12	S	s	NN
cord-014597-66vd2mdu	1855	13	-	-	HYPH
cord-014597-66vd2mdu	1855	14	phase	phase	NN
cord-014597-66vd2mdu	1855	15	cells	cell	NNS
cord-014597-66vd2mdu	1855	16	were	be	VBD
cord-014597-66vd2mdu	1855	17	enriched	enrich	VBN
cord-014597-66vd2mdu	1855	18	at	at	IN
cord-014597-66vd2mdu	1855	19	least	least	JJS
cord-014597-66vd2mdu	1855	20	temporary	temporary	JJ
cord-014597-66vd2mdu	1855	21	(	(	-LRB-
cord-014597-66vd2mdu	1855	22	Fig	fig	NN
cord-014597-66vd2mdu	1855	23	.	.	.
cord-014597-66vd2mdu	1855	24	1a	1a	CD
cord-014597-66vd2mdu	1855	25	)	)	-RRB-
cord-014597-66vd2mdu	1855	26	.	.	.
cord-014597-66vd2mdu	1856	1	Regarding	regard	VBG
cord-014597-66vd2mdu	1856	2	antibody	antibody	NN
cord-014597-66vd2mdu	1856	3	production	production	NN
cord-014597-66vd2mdu	1856	4	IGF	IGF	NNP
cord-014597-66vd2mdu	1856	5	led	lead	VBD
cord-014597-66vd2mdu	1856	6	to	to	IN
cord-014597-66vd2mdu	1856	7	a	a	DT
cord-014597-66vd2mdu	1856	8	decreased	decrease	VBN
cord-014597-66vd2mdu	1856	9	qP	qP	NNP
cord-014597-66vd2mdu	1856	10	and	and	CC
cord-014597-66vd2mdu	1856	11	product	product	NN
cord-014597-66vd2mdu	1856	12	titer	titer	NN
cord-014597-66vd2mdu	1856	13	,	,	,
cord-014597-66vd2mdu	1856	14	concomitantly	concomitantly	RB
cord-014597-66vd2mdu	1856	15	with	with	IN
cord-014597-66vd2mdu	1856	16	an	an	DT
cord-014597-66vd2mdu	1856	17	increase	increase	NN
cord-014597-66vd2mdu	1856	18	in	in	IN
cord-014597-66vd2mdu	1856	19	S	s	NN
cord-014597-66vd2mdu	1856	20	-	-	HYPH
cord-014597-66vd2mdu	1856	21	phase	phase	NN
cord-014597-66vd2mdu	1856	22	cells	cell	NNS
cord-014597-66vd2mdu	1856	23	(	(	-LRB-
cord-014597-66vd2mdu	1856	24	Fig	fig	NN
cord-014597-66vd2mdu	1856	25	.	.	.
cord-014597-66vd2mdu	1856	26	 	 	_SP
cord-014597-66vd2mdu	1856	27	1a	1a	CD
cord-014597-66vd2mdu	1856	28	)	)	-RRB-
cord-014597-66vd2mdu	1856	29	.	.	.
cord-014597-66vd2mdu	1857	1	This	this	DT
cord-014597-66vd2mdu	1857	2	inverse	inverse	JJ
cord-014597-66vd2mdu	1857	3	correlation	correlation	NN
cord-014597-66vd2mdu	1857	4	of	of	IN
cord-014597-66vd2mdu	1857	5	proliferation	proliferation	NN
cord-014597-66vd2mdu	1857	6	and	and	CC
cord-014597-66vd2mdu	1857	7	cell	cell	NN
cord-014597-66vd2mdu	1857	8	specific	specific	JJ
cord-014597-66vd2mdu	1857	9	productivity	productivity	NN
cord-014597-66vd2mdu	1857	10	is	be	VBZ
cord-014597-66vd2mdu	1857	11	known	know	VBN
cord-014597-66vd2mdu	1857	12	from	from	IN
cord-014597-66vd2mdu	1857	13	different	different	JJ
cord-014597-66vd2mdu	1857	14	productivity	productivity	NN
cord-014597-66vd2mdu	1857	15	enhancing	enhance	VBG
cord-014597-66vd2mdu	1857	16	molecules	molecule	NNS
cord-014597-66vd2mdu	1857	17	,	,	,
cord-014597-66vd2mdu	1857	18	like	like	IN
cord-014597-66vd2mdu	1857	19	butyrate	butyrate	NN
cord-014597-66vd2mdu	1857	20	[	[	-LRB-
cord-014597-66vd2mdu	1857	21	3	3	CD
cord-014597-66vd2mdu	1857	22	]	]	-RRB-
cord-014597-66vd2mdu	1857	23	.	.	.
cord-014597-66vd2mdu	1858	1	MS	MS	NNP
cord-014597-66vd2mdu	1858	2	investigation	investigation	NN
cord-014597-66vd2mdu	1858	3	of	of	IN
cord-014597-66vd2mdu	1858	4	signaling	signal	VBG
cord-014597-66vd2mdu	1858	5	events	event	NNS
cord-014597-66vd2mdu	1858	6	The	the	DT
cord-014597-66vd2mdu	1858	7	phosphoproteomic	phosphoproteomic	JJ
cord-014597-66vd2mdu	1858	8	experiment	experiment	NN
cord-014597-66vd2mdu	1858	9	resulted	result	VBD
cord-014597-66vd2mdu	1858	10	in	in	IN
cord-014597-66vd2mdu	1858	11	the	the	DT
cord-014597-66vd2mdu	1858	12	identification	identification	NN
cord-014597-66vd2mdu	1858	13	of	of	IN
cord-014597-66vd2mdu	1858	14	10.485	10.485	CD
cord-014597-66vd2mdu	1858	15	class	class	NN
cord-014597-66vd2mdu	1858	16	I	i	NN
cord-014597-66vd2mdu	1858	17	-	-	HYPH
cord-014597-66vd2mdu	1858	18	phosphorylation	phosphorylation	NN
cord-014597-66vd2mdu	1858	19	sites	site	NNS
cord-014597-66vd2mdu	1858	20	.	.	.
cord-014597-66vd2mdu	1859	1	Statistical	statistical	JJ
cord-014597-66vd2mdu	1859	2	evaluation	evaluation	NN
cord-014597-66vd2mdu	1859	3	of	of	IN
cord-014597-66vd2mdu	1859	4	phosphopeptide	phosphopeptide	JJ
cord-014597-66vd2mdu	1859	5	abundances	abundance	NNS
cord-014597-66vd2mdu	1859	6	in	in	IN
cord-014597-66vd2mdu	1859	7	Perseus	Perseus	NNP
cord-014597-66vd2mdu	1859	8	showed	show	VBD
cord-014597-66vd2mdu	1859	9	up	up	RP
cord-014597-66vd2mdu	1859	10	144	144	CD
cord-014597-66vd2mdu	1859	11	significant	significant	JJ
cord-014597-66vd2mdu	1859	12	differences	difference	NNS
cord-014597-66vd2mdu	1859	13	between	between	IN
cord-014597-66vd2mdu	1859	14	the	the	DT
cord-014597-66vd2mdu	1859	15	cell	cell	NN
cord-014597-66vd2mdu	1859	16	lines	line	NNS
cord-014597-66vd2mdu	1859	17	and	and	CC
cord-014597-66vd2mdu	1859	18	led	lead	VBD
cord-014597-66vd2mdu	1859	19	to	to	IN
cord-014597-66vd2mdu	1859	20	producer	producer	NN
cord-014597-66vd2mdu	1859	21	vs.	vs.	FW
cord-014597-66vd2mdu	1859	22	parental	parental	JJ
cord-014597-66vd2mdu	1859	23	classifications	classification	NNS
cord-014597-66vd2mdu	1859	24	(	(	-LRB-
cord-014597-66vd2mdu	1859	25	Fig	Fig	NNP
cord-014597-66vd2mdu	1859	26	.	.	.
cord-014597-66vd2mdu	1859	27	1b	1b	CD
cord-014597-66vd2mdu	1859	28	)	)	-RRB-
cord-014597-66vd2mdu	1859	29	.	.	.
cord-014597-66vd2mdu	1860	1	The	the	DT
cord-014597-66vd2mdu	1860	2	quantitative	quantitative	JJ
cord-014597-66vd2mdu	1860	3	evaluation	evaluation	NN
cord-014597-66vd2mdu	1860	4	via	via	IN
cord-014597-66vd2mdu	1860	5	SILAC	SILAC	NNP
cord-014597-66vd2mdu	1860	6	yielded	yield	VBD
cord-014597-66vd2mdu	1860	7	in	in	IN
cord-014597-66vd2mdu	1860	8	about	about	RB
cord-014597-66vd2mdu	1860	9	2.408	2.408	CD
cord-014597-66vd2mdu	1860	10	quantifiable	quantifiable	JJ
cord-014597-66vd2mdu	1860	11	phosphosites	phosphosite	NNS
cord-014597-66vd2mdu	1860	12	in	in	IN
cord-014597-66vd2mdu	1860	13	at	at	RB
cord-014597-66vd2mdu	1860	14	least	least	RBS
cord-014597-66vd2mdu	1860	15	6	6	CD
cord-014597-66vd2mdu	1860	16	biological	biological	JJ
cord-014597-66vd2mdu	1860	17	replicates	replicate	NNS
cord-014597-66vd2mdu	1860	18	.	.	.
cord-014597-66vd2mdu	1861	1	Rapid	rapid	JJ
cord-014597-66vd2mdu	1861	2	phosphorylation	phosphorylation	NN
cord-014597-66vd2mdu	1861	3	changes	change	NNS
cord-014597-66vd2mdu	1861	4	after	after	IN
cord-014597-66vd2mdu	1861	5	growth	growth	NN
cord-014597-66vd2mdu	1861	6	factor	factor	NN
cord-014597-66vd2mdu	1861	7	treatment	treatment	NN
cord-014597-66vd2mdu	1861	8	indicated	indicate	VBD
cord-014597-66vd2mdu	1861	9	signaling	signal	VBG
cord-014597-66vd2mdu	1861	10	towards	towards	IN
cord-014597-66vd2mdu	1861	11	protein	protein	NN
cord-014597-66vd2mdu	1861	12	synthesis	synthesis	NN
cord-014597-66vd2mdu	1861	13	,	,	,
cord-014597-66vd2mdu	1861	14	cell	cell	NN
cord-014597-66vd2mdu	1861	15	cycle	cycle	NN
cord-014597-66vd2mdu	1861	16	and	and	CC
cord-014597-66vd2mdu	1861	17	regulation	regulation	NN
cord-014597-66vd2mdu	1861	18	of	of	IN
cord-014597-66vd2mdu	1861	19	actin	actin	NN
cord-014597-66vd2mdu	1861	20	cytoskeleton	cytoskeleton	NN
cord-014597-66vd2mdu	1861	21	amongst	amongst	IN
cord-014597-66vd2mdu	1861	22	others	other	NNS
cord-014597-66vd2mdu	1861	23	.	.	.
cord-014597-66vd2mdu	1862	1	For	for	IN
cord-014597-66vd2mdu	1862	2	201	201	CD
cord-014597-66vd2mdu	1862	3	phosphosites	phosphosite	NNS
cord-014597-66vd2mdu	1862	4	significantly	significantly	RB
cord-014597-66vd2mdu	1862	5	different	different	JJ
cord-014597-66vd2mdu	1862	6	H	H	NNP
cord-014597-66vd2mdu	1862	7	/	/	SYM
cord-014597-66vd2mdu	1862	8	L	l	NN
cord-014597-66vd2mdu	1862	9	ratios	ratio	NNS
cord-014597-66vd2mdu	1862	10	were	be	VBD
cord-014597-66vd2mdu	1862	11	calculated	calculate	VBN
cord-014597-66vd2mdu	1862	12	between	between	IN
cord-014597-66vd2mdu	1862	13	the	the	DT
cord-014597-66vd2mdu	1862	14	two	two	CD
cord-014597-66vd2mdu	1862	15	groups	group	NNS
cord-014597-66vd2mdu	1862	16	parental	parental	JJ
cord-014597-66vd2mdu	1862	17	vs.	vs.	NN
cord-014597-66vd2mdu	1862	18	producer	producer	NN
cord-014597-66vd2mdu	1862	19	,	,	,
cord-014597-66vd2mdu	1862	20	four	four	CD
cord-014597-66vd2mdu	1862	21	of	of	IN
cord-014597-66vd2mdu	1862	22	them	-PRON-	PRP
cord-014597-66vd2mdu	1862	23	are	be	VBP
cord-014597-66vd2mdu	1862	24	listed	list	VBN
cord-014597-66vd2mdu	1863	1	(	(	-LRB-
cord-014597-66vd2mdu	1863	2	Table	table	NN
cord-014597-66vd2mdu	1863	3	1	1	CD
cord-014597-66vd2mdu	1863	4	)	)	-RRB-
cord-014597-66vd2mdu	1863	5	.	.	.
cord-014597-66vd2mdu	1864	1	The	the	DT
cord-014597-66vd2mdu	1864	2	workflow	workflow	NN
cord-014597-66vd2mdu	1864	3	to	to	TO
cord-014597-66vd2mdu	1864	4	study	study	VB
cord-014597-66vd2mdu	1864	5	phosphorylation	phosphorylation	NN
cord-014597-66vd2mdu	1864	6	states	state	NNS
cord-014597-66vd2mdu	1864	7	revealed	reveal	VBD
cord-014597-66vd2mdu	1864	8	differences	difference	NNS
cord-014597-66vd2mdu	1864	9	in	in	IN
cord-014597-66vd2mdu	1864	10	the	the	DT
cord-014597-66vd2mdu	1864	11	related	related	JJ
cord-014597-66vd2mdu	1864	12	cell	cell	NN
cord-014597-66vd2mdu	1864	13	lines	line	NNS
cord-014597-66vd2mdu	1864	14	and	and	CC
cord-014597-66vd2mdu	1864	15	gave	give	VBD
cord-014597-66vd2mdu	1864	16	insights	insight	NNS
cord-014597-66vd2mdu	1864	17	into	into	IN
cord-014597-66vd2mdu	1864	18	signal	signal	NN
cord-014597-66vd2mdu	1864	19	transduction	transduction	NN
cord-014597-66vd2mdu	1864	20	as	as	IN
cord-014597-66vd2mdu	1864	21	a	a	DT
cord-014597-66vd2mdu	1864	22	response	response	NN
cord-014597-66vd2mdu	1864	23	on	on	IN
cord-014597-66vd2mdu	1864	24	IGF	IGF	NNP
cord-014597-66vd2mdu	1864	25	.	.	.
cord-014597-66vd2mdu	1865	1	On	on	IN
cord-014597-66vd2mdu	1865	2	the	the	DT
cord-014597-66vd2mdu	1865	3	one	one	CD
cord-014597-66vd2mdu	1865	4	hand	hand	NN
cord-014597-66vd2mdu	1865	5	,	,	,
cord-014597-66vd2mdu	1865	6	IGF	IGF	NNP
cord-014597-66vd2mdu	1865	7	-	-	HYPH
cord-014597-66vd2mdu	1865	8	treatment	treatment	NN
cord-014597-66vd2mdu	1865	9	resulted	result	VBD
cord-014597-66vd2mdu	1865	10	in	in	IN
cord-014597-66vd2mdu	1865	11	a	a	DT
cord-014597-66vd2mdu	1865	12	fast	fast	JJ
cord-014597-66vd2mdu	1865	13	and	and	CC
cord-014597-66vd2mdu	1865	14	widespread	widespread	JJ
cord-014597-66vd2mdu	1865	15	upregulation	upregulation	NN
cord-014597-66vd2mdu	1865	16	of	of	IN
cord-014597-66vd2mdu	1865	17	phosphorylation	phosphorylation	NN
cord-014597-66vd2mdu	1865	18	sites	site	NNS
cord-014597-66vd2mdu	1865	19	within	within	IN
cord-014597-66vd2mdu	1865	20	Aktand	Aktand	NNP
cord-014597-66vd2mdu	1865	21	MAPK	MAPK	NNP
cord-014597-66vd2mdu	1865	22	-	-	HYPH
cord-014597-66vd2mdu	1865	23	signaling	signal	VBG
cord-014597-66vd2mdu	1865	24	.	.	.
cord-014597-66vd2mdu	1866	1	On	on	IN
cord-014597-66vd2mdu	1866	2	the	the	DT
cord-014597-66vd2mdu	1866	3	other	other	JJ
cord-014597-66vd2mdu	1866	4	hand	hand	NN
cord-014597-66vd2mdu	1866	5	,	,	,
cord-014597-66vd2mdu	1866	6	a	a	DT
cord-014597-66vd2mdu	1866	7	different	different	JJ
cord-014597-66vd2mdu	1866	8	phosphorylation	phosphorylation	NN
cord-014597-66vd2mdu	1866	9	status	status	NN
cord-014597-66vd2mdu	1866	10	for	for	IN
cord-014597-66vd2mdu	1866	11	producer	producer	NN
cord-014597-66vd2mdu	1866	12	compared	compare	VBN
cord-014597-66vd2mdu	1866	13	to	to	IN
cord-014597-66vd2mdu	1866	14	parental	parental	JJ
cord-014597-66vd2mdu	1866	15	cell	cell	NN
cord-014597-66vd2mdu	1866	16	lines	line	NNS
cord-014597-66vd2mdu	1866	17	uncovered	uncover	VBD
cord-014597-66vd2mdu	1866	18	distinctions	distinction	NNS
cord-014597-66vd2mdu	1866	19	in	in	IN
cord-014597-66vd2mdu	1866	20	biological	biological	JJ
cord-014597-66vd2mdu	1866	21	processes	process	NNS
cord-014597-66vd2mdu	1866	22	like	like	IN
cord-014597-66vd2mdu	1866	23	RNA	RNA	NNP
cord-014597-66vd2mdu	1866	24	-	-	:
cord-014597-66vd2mdu	1866	25	and	and	CC
cord-014597-66vd2mdu	1866	26	DNA	dna	NN
cord-014597-66vd2mdu	1866	27	-	-	HYPH
cord-014597-66vd2mdu	1866	28	binding	binding	NN
cord-014597-66vd2mdu	1866	29	and	and	CC
cord-014597-66vd2mdu	1866	30	regulation	regulation	NN
cord-014597-66vd2mdu	1866	31	of	of	IN
cord-014597-66vd2mdu	1866	32	cytoskeleton	cytoskeleton	NN
cord-014597-66vd2mdu	1866	33	.	.	.
cord-014597-66vd2mdu	1867	1	In	in	IN
cord-014597-66vd2mdu	1867	2	sum	sum	NN
cord-014597-66vd2mdu	1867	3	,	,	,
cord-014597-66vd2mdu	1867	4	our	-PRON-	PRP$
cord-014597-66vd2mdu	1867	5	sucessfully	sucessfully	RB
cord-014597-66vd2mdu	1867	6	established	establish	VBN
cord-014597-66vd2mdu	1867	7	phosphoproteomic	phosphoproteomic	JJ
cord-014597-66vd2mdu	1867	8	approach	approach	NN
cord-014597-66vd2mdu	1867	9	allows	allow	VBZ
cord-014597-66vd2mdu	1867	10	to	to	TO
cord-014597-66vd2mdu	1867	11	detect	detect	VB
cord-014597-66vd2mdu	1867	12	important	important	JJ
cord-014597-66vd2mdu	1867	13	signaling	signal	VBG
cord-014597-66vd2mdu	1867	14	key	key	JJ
cord-014597-66vd2mdu	1867	15	players	player	NNS
cord-014597-66vd2mdu	1867	16	in	in	IN
cord-014597-66vd2mdu	1867	17	CHO	CHO	NNP
cord-014597-66vd2mdu	1867	18	cells	cell	NNS
cord-014597-66vd2mdu	1867	19	that	that	WDT
cord-014597-66vd2mdu	1867	20	subsequently	subsequently	RB
cord-014597-66vd2mdu	1867	21	can	can	MD
cord-014597-66vd2mdu	1867	22	be	be	VB
cord-014597-66vd2mdu	1867	23	targeted	target	VBN
cord-014597-66vd2mdu	1867	24	through	through	IN
cord-014597-66vd2mdu	1867	25	cell	cell	NN
cord-014597-66vd2mdu	1867	26	engineering	engineering	NN
cord-014597-66vd2mdu	1867	27	or	or	CC
cord-014597-66vd2mdu	1867	28	small	small	JJ
cord-014597-66vd2mdu	1867	29	molecule	molecule	NN
cord-014597-66vd2mdu	1867	30	treatment	treatment	NN
cord-014597-66vd2mdu	1867	31	.	.	.
cord-014597-66vd2mdu	1868	1	To	to	TO
cord-014597-66vd2mdu	1868	2	improve	improve	VB
cord-014597-66vd2mdu	1868	3	antibody	antibody	NN
cord-014597-66vd2mdu	1868	4	production	production	NN
cord-014597-66vd2mdu	1868	5	in	in	IN
cord-014597-66vd2mdu	1868	6	the	the	DT
cord-014597-66vd2mdu	1868	7	CHO	CHO	NNP
cord-014597-66vd2mdu	1868	8	cell	cell	NN
cord-014597-66vd2mdu	1868	9	expression	expression	NN
cord-014597-66vd2mdu	1868	10	system	system	NN
cord-014597-66vd2mdu	1868	11	,	,	,
cord-014597-66vd2mdu	1868	12	it	-PRON-	PRP
cord-014597-66vd2mdu	1868	13	seems	seem	VBZ
cord-014597-66vd2mdu	1868	14	to	to	TO
cord-014597-66vd2mdu	1868	15	be	be	VB
cord-014597-66vd2mdu	1868	16	useful	useful	JJ
cord-014597-66vd2mdu	1868	17	to	to	TO
cord-014597-66vd2mdu	1868	18	up	up	IN
cord-014597-66vd2mdu	1868	19	-	-	:
cord-014597-66vd2mdu	1868	20	or	or	CC
cord-014597-66vd2mdu	1868	21	downregulate	downregulate	VB
cord-014597-66vd2mdu	1868	22	gene	gene	NN
cord-014597-66vd2mdu	1868	23	expression	expression	NN
cord-014597-66vd2mdu	1868	24	including	include	VBG
cord-014597-66vd2mdu	1868	25	antibody	antibody	NN
cord-014597-66vd2mdu	1868	26	folding	folding	NN
cord-014597-66vd2mdu	1868	27	,	,	,
cord-014597-66vd2mdu	1868	28	secretion	secretion	NN
cord-014597-66vd2mdu	1868	29	,	,	,
cord-014597-66vd2mdu	1868	30	and	and	CC
cord-014597-66vd2mdu	1868	31	cell	cell	NN
cord-014597-66vd2mdu	1868	32	metabolism	metabolism	NN
cord-014597-66vd2mdu	1868	33	.	.	.
cord-014597-66vd2mdu	1869	1	Many	many	JJ
cord-014597-66vd2mdu	1869	2	cell	cell	NN
cord-014597-66vd2mdu	1869	3	engineering	engineering	NN
cord-014597-66vd2mdu	1869	4	approaches	approach	NNS
cord-014597-66vd2mdu	1869	5	,	,	,
cord-014597-66vd2mdu	1869	6	including	include	VBG
cord-014597-66vd2mdu	1869	7	gene	gene	NN
cord-014597-66vd2mdu	1869	8	introduction	introduction	NN
cord-014597-66vd2mdu	1869	9	,	,	,
cord-014597-66vd2mdu	1869	10	knockout	knockout	NN
cord-014597-66vd2mdu	1869	11	and	and	CC
cord-014597-66vd2mdu	1869	12	knockdown	knockdown	NN
cord-014597-66vd2mdu	1869	13	,	,	,
cord-014597-66vd2mdu	1869	14	have	have	VBP
cord-014597-66vd2mdu	1869	15	been	be	VBN
cord-014597-66vd2mdu	1869	16	employed	employ	VBN
cord-014597-66vd2mdu	1869	17	to	to	TO
cord-014597-66vd2mdu	1869	18	enhance	enhance	VB
cord-014597-66vd2mdu	1869	19	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1869	20	antibody	antibody	NN
cord-014597-66vd2mdu	1869	21	production	production	NN
cord-014597-66vd2mdu	1869	22	[	[	-LRB-
cord-014597-66vd2mdu	1869	23	1	1	CD
cord-014597-66vd2mdu	1869	24	]	]	-RRB-
cord-014597-66vd2mdu	1869	25	.	.	.
cord-014597-66vd2mdu	1870	1	However	however	RB
cord-014597-66vd2mdu	1870	2	,	,	,
cord-014597-66vd2mdu	1870	3	identifying	identify	VBG
cord-014597-66vd2mdu	1870	4	production	production	NN
cord-014597-66vd2mdu	1870	5	enhancer	enhancer	NN
cord-014597-66vd2mdu	1870	6	genes	gene	NNS
cord-014597-66vd2mdu	1870	7	is	be	VBZ
cord-014597-66vd2mdu	1870	8	the	the	DT
cord-014597-66vd2mdu	1870	9	rate	rate	NN
cord-014597-66vd2mdu	1870	10	-	-	HYPH
cord-014597-66vd2mdu	1870	11	limiting	limit	VBG
cord-014597-66vd2mdu	1870	12	step	step	NN
cord-014597-66vd2mdu	1870	13	for	for	IN
cord-014597-66vd2mdu	1870	14	CHO	CHO	NNP
cord-014597-66vd2mdu	1870	15	cell	cell	NN
cord-014597-66vd2mdu	1870	16	engineering	engineering	NN
cord-014597-66vd2mdu	1870	17	,	,	,
cord-014597-66vd2mdu	1870	18	because	because	IN
cord-014597-66vd2mdu	1870	19	the	the	DT
cord-014597-66vd2mdu	1870	20	conventional	conventional	JJ
cord-014597-66vd2mdu	1870	21	method	method	NN
cord-014597-66vd2mdu	1870	22	requires	require	VBZ
cord-014597-66vd2mdu	1870	23	a	a	DT
cord-014597-66vd2mdu	1870	24	series	series	NN
cord-014597-66vd2mdu	1870	25	of	of	IN
cord-014597-66vd2mdu	1870	26	experiments	experiment	NNS
cord-014597-66vd2mdu	1870	27	including	include	VBG
cord-014597-66vd2mdu	1870	28	genomic	genomic	JJ
cord-014597-66vd2mdu	1870	29	integration	integration	NN
cord-014597-66vd2mdu	1870	30	of	of	IN
cord-014597-66vd2mdu	1870	31	the	the	DT
cord-014597-66vd2mdu	1870	32	tested	test	VBN
cord-014597-66vd2mdu	1870	33	genes	gene	NNS
cord-014597-66vd2mdu	1870	34	,	,	,
cord-014597-66vd2mdu	1870	35	selection	selection	NN
cord-014597-66vd2mdu	1870	36	of	of	IN
cord-014597-66vd2mdu	1870	37	stable	stable	JJ
cord-014597-66vd2mdu	1870	38	cell	cell	NN
cord-014597-66vd2mdu	1870	39	clones	clone	NNS
cord-014597-66vd2mdu	1870	40	and	and	CC
cord-014597-66vd2mdu	1870	41	cell	cell	NN
cord-014597-66vd2mdu	1870	42	culture	culture	NN
cord-014597-66vd2mdu	1870	43	experiments	experiment	NNS
cord-014597-66vd2mdu	1870	44	of	of	IN
cord-014597-66vd2mdu	1870	45	several	several	JJ
cord-014597-66vd2mdu	1870	46	clones	clone	NNS
cord-014597-66vd2mdu	1870	47	.	.	.
cord-014597-66vd2mdu	1871	1	In	in	IN
cord-014597-66vd2mdu	1871	2	this	this	DT
cord-014597-66vd2mdu	1871	3	study	study	NN
cord-014597-66vd2mdu	1871	4	,	,	,
cord-014597-66vd2mdu	1871	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1871	6	propose	propose	VBP
cord-014597-66vd2mdu	1871	7	an	an	DT
cord-014597-66vd2mdu	1871	8	approach	approach	NN
cord-014597-66vd2mdu	1871	9	for	for	IN
cord-014597-66vd2mdu	1871	10	rapid	rapid	JJ
cord-014597-66vd2mdu	1871	11	evaluation	evaluation	NN
cord-014597-66vd2mdu	1871	12	of	of	IN
cord-014597-66vd2mdu	1871	13	production	production	NN
cord-014597-66vd2mdu	1871	14	enhancer	enhancer	NN
cord-014597-66vd2mdu	1871	15	genes	gene	NNS
cord-014597-66vd2mdu	1871	16	based	base	VBN
cord-014597-66vd2mdu	1871	17	on	on	IN
cord-014597-66vd2mdu	1871	18	an	an	DT
cord-014597-66vd2mdu	1871	19	episomal	episomal	JJ
cord-014597-66vd2mdu	1871	20	expression	expression	NN
cord-014597-66vd2mdu	1871	21	system	system	NN
cord-014597-66vd2mdu	1871	22	.	.	.
cord-014597-66vd2mdu	1872	1	Plasmid	plasmid	NN
cord-014597-66vd2mdu	1872	2	vector	vector	NN
cord-014597-66vd2mdu	1872	3	carrying	carry	VBG
cord-014597-66vd2mdu	1872	4	the	the	DT
cord-014597-66vd2mdu	1872	5	Epstein	Epstein	NNP
cord-014597-66vd2mdu	1872	6	-	-	HYPH
cord-014597-66vd2mdu	1872	7	Barr	Barr	NNP
cord-014597-66vd2mdu	1872	8	virus	virus	NN
cord-014597-66vd2mdu	1872	9	(	(	-LRB-
cord-014597-66vd2mdu	1872	10	EBV	EBV	NNP
cord-014597-66vd2mdu	1872	11	)	)	-RRB-
cord-014597-66vd2mdu	1872	12	encoded	encode	VBD
cord-014597-66vd2mdu	1872	13	nuclear	nuclear	JJ
cord-014597-66vd2mdu	1872	14	antigen	antigen	NN
cord-014597-66vd2mdu	1872	15	1	1	CD
cord-014597-66vd2mdu	1873	1	(	(	-LRB-
cord-014597-66vd2mdu	1873	2	EBNA1	EBNA1	NNP
cord-014597-66vd2mdu	1873	3	)	)	-RRB-
cord-014597-66vd2mdu	1873	4	was	be	VBD
cord-014597-66vd2mdu	1873	5	transfected	transfecte	VBN
cord-014597-66vd2mdu	1873	6	into	into	IN
cord-014597-66vd2mdu	1873	7	CHO	CHO	NNP
cord-014597-66vd2mdu	1873	8	cell	cell	NN
cord-014597-66vd2mdu	1873	9	line	line	NN
cord-014597-66vd2mdu	1873	10	producing	produce	VBG
cord-014597-66vd2mdu	1873	11	IgG1	IgG1	NNP
cord-014597-66vd2mdu	1873	12	antibody	antibody	NN
cord-014597-66vd2mdu	1873	13	.	.	.
cord-014597-66vd2mdu	1874	1	After	after	IN
cord-014597-66vd2mdu	1874	2	G418	G418	NNPS
cord-014597-66vd2mdu	1874	3	selection	selection	NN
cord-014597-66vd2mdu	1874	4	and	and	CC
cord-014597-66vd2mdu	1874	5	single	single	JJ
cord-014597-66vd2mdu	1874	6	colony	colony	NN
cord-014597-66vd2mdu	1874	7	isolation	isolation	NN
cord-014597-66vd2mdu	1874	8	,	,	,
cord-014597-66vd2mdu	1874	9	EBNA1	EBNA1	NNS
cord-014597-66vd2mdu	1874	10	expression	expression	NN
cord-014597-66vd2mdu	1874	11	was	be	VBD
cord-014597-66vd2mdu	1874	12	checked	check	VBN
cord-014597-66vd2mdu	1874	13	with	with	IN
cord-014597-66vd2mdu	1874	14	capillary	capillary	JJ
cord-014597-66vd2mdu	1874	15	electrophoresis	electrophoresis	NN
cord-014597-66vd2mdu	1874	16	system	system	NN
cord-014597-66vd2mdu	1874	17	Wes	Wes	NNP
cord-014597-66vd2mdu	1874	18	(	(	-LRB-
cord-014597-66vd2mdu	1874	19	ProteinSimple	ProteinSimple	NNP
cord-014597-66vd2mdu	1874	20	)	)	-RRB-
cord-014597-66vd2mdu	1874	21	.	.	.
cord-014597-66vd2mdu	1875	1	EBV	EBV	NNP
cord-014597-66vd2mdu	1875	2	EBNA1-antibody	EBNA1-antibody	NNP
cord-014597-66vd2mdu	1875	3	(	(	-LRB-
cord-014597-66vd2mdu	1875	4	1EB12	1EB12	NNS
cord-014597-66vd2mdu	1875	5	)	)	-RRB-
cord-014597-66vd2mdu	1875	6	was	be	VBD
cord-014597-66vd2mdu	1875	7	used	use	VBN
cord-014597-66vd2mdu	1875	8	for	for	IN
cord-014597-66vd2mdu	1875	9	detection	detection	NN
cord-014597-66vd2mdu	1875	10	as	as	IN
cord-014597-66vd2mdu	1875	11	the	the	DT
cord-014597-66vd2mdu	1875	12	primary	primary	JJ
cord-014597-66vd2mdu	1875	13	antibody	antibody	NN
cord-014597-66vd2mdu	1875	14	.	.	.
cord-014597-66vd2mdu	1876	1	The	the	DT
cord-014597-66vd2mdu	1876	2	expression	expression	NN
cord-014597-66vd2mdu	1876	3	vector	vector	NN
cord-014597-66vd2mdu	1876	4	for	for	IN
cord-014597-66vd2mdu	1876	5	the	the	DT
cord-014597-66vd2mdu	1876	6	gene	gene	NN
cord-014597-66vd2mdu	1876	7	of	of	IN
cord-014597-66vd2mdu	1876	8	interest	interest	NN
cord-014597-66vd2mdu	1876	9	was	be	VBD
cord-014597-66vd2mdu	1876	10	prepared	prepare	VBN
cord-014597-66vd2mdu	1876	11	by	by	IN
cord-014597-66vd2mdu	1876	12	inserting	insert	VBG
cord-014597-66vd2mdu	1876	13	1508	1508	CD
cord-014597-66vd2mdu	1876	14	bp	bp	NNP
cord-014597-66vd2mdu	1876	15	of	of	IN
cord-014597-66vd2mdu	1876	16	an	an	DT
cord-014597-66vd2mdu	1876	17	oriP	orip	CD
cord-014597-66vd2mdu	1876	18	DNA	dna	NN
cord-014597-66vd2mdu	1876	19	sequence	sequence	NN
cord-014597-66vd2mdu	1876	20	into	into	IN
cord-014597-66vd2mdu	1876	21	a	a	DT
cord-014597-66vd2mdu	1876	22	plasmid	plasmid	NN
cord-014597-66vd2mdu	1876	23	vector	vector	NN
cord-014597-66vd2mdu	1876	24	carrying	carry	VBG
cord-014597-66vd2mdu	1876	25	CAG	CAG	NNP
cord-014597-66vd2mdu	1876	26	promoter	promoter	NN
cord-014597-66vd2mdu	1876	27	,	,	,
cord-014597-66vd2mdu	1876	28	resulting	result	VBG
cord-014597-66vd2mdu	1876	29	in	in	IN
cord-014597-66vd2mdu	1876	30	the	the	DT
cord-014597-66vd2mdu	1876	31	pOTC	potc	NN
cord-014597-66vd2mdu	1876	32	vector	vector	NN
cord-014597-66vd2mdu	1876	33	.	.	.
cord-014597-66vd2mdu	1877	1	PEI	PEI	NNP
cord-014597-66vd2mdu	1877	2	max	max	NNP
cord-014597-66vd2mdu	1877	3	(	(	-LRB-
cord-014597-66vd2mdu	1877	4	Polysciences	Polysciences	NNP
cord-014597-66vd2mdu	1877	5	,	,	,
cord-014597-66vd2mdu	1877	6	Inc.	Inc.	NNP
cord-014597-66vd2mdu	1877	7	)	)	-RRB-
cord-014597-66vd2mdu	1877	8	and	and	CC
cord-014597-66vd2mdu	1877	9	BalanCD	BalanCD	NNP
cord-014597-66vd2mdu	1877	10	Transfectory	Transfectory	NNP
cord-014597-66vd2mdu	1877	11	CHO	CHO	NNP
cord-014597-66vd2mdu	1877	12	(	(	-LRB-
cord-014597-66vd2mdu	1877	13	Irvine	Irvine	NNP
cord-014597-66vd2mdu	1877	14	Scientific	Scientific	NNP
cord-014597-66vd2mdu	1877	15	)	)	-RRB-
cord-014597-66vd2mdu	1877	16	were	be	VBD
cord-014597-66vd2mdu	1877	17	used	use	VBN
cord-014597-66vd2mdu	1877	18	for	for	IN
cord-014597-66vd2mdu	1877	19	the	the	DT
cord-014597-66vd2mdu	1877	20	transfection	transfection	NN
cord-014597-66vd2mdu	1877	21	.	.	.
cord-014597-66vd2mdu	1878	1	The	the	DT
cord-014597-66vd2mdu	1878	2	number	number	NN
cord-014597-66vd2mdu	1878	3	of	of	IN
cord-014597-66vd2mdu	1878	4	viable	viable	JJ
cord-014597-66vd2mdu	1878	5	cells	cell	NNS
cord-014597-66vd2mdu	1878	6	and	and	CC
cord-014597-66vd2mdu	1878	7	GFP	gfp	NN
cord-014597-66vd2mdu	1878	8	-	-	HYPH
cord-014597-66vd2mdu	1878	9	positive	positive	JJ
cord-014597-66vd2mdu	1878	10	cells	cell	NNS
cord-014597-66vd2mdu	1878	11	were	be	VBD
cord-014597-66vd2mdu	1878	12	counted	count	VBN
cord-014597-66vd2mdu	1878	13	using	use	VBG
cord-014597-66vd2mdu	1878	14	Countess	Countess	NNP
cord-014597-66vd2mdu	1878	15	II	II	NNP
cord-014597-66vd2mdu	1878	16	FL	FL	NNP
cord-014597-66vd2mdu	1878	17	Automated	Automated	NNP
cord-014597-66vd2mdu	1878	18	Cell	Cell	NNP
cord-014597-66vd2mdu	1878	19	Counter	Counter	NNP
cord-014597-66vd2mdu	1878	20	(	(	-LRB-
cord-014597-66vd2mdu	1878	21	Thermo	Thermo	NNP
cord-014597-66vd2mdu	1878	22	Fisher	Fisher	NNP
cord-014597-66vd2mdu	1878	23	Scientific	Scientific	NNP
cord-014597-66vd2mdu	1878	24	)	)	-RRB-
cord-014597-66vd2mdu	1878	25	.	.	.
cord-014597-66vd2mdu	1879	1	The	the	DT
cord-014597-66vd2mdu	1879	2	transfected	transfecte	VBN
cord-014597-66vd2mdu	1879	3	cells	cell	NNS
cord-014597-66vd2mdu	1879	4	were	be	VBD
cord-014597-66vd2mdu	1879	5	cultured	culture	VBN
cord-014597-66vd2mdu	1879	6	in	in	IN
cord-014597-66vd2mdu	1879	7	CELLSTAR	CELLSTAR	NNP
cord-014597-66vd2mdu	1879	8	CELLreactor	CELLreactor	NNP
cord-014597-66vd2mdu	1879	9	tubes	tube	NNS
cord-014597-66vd2mdu	1879	10	.	.	.
cord-014597-66vd2mdu	1880	1	The	the	DT
cord-014597-66vd2mdu	1880	2	tubes	tube	NNS
cord-014597-66vd2mdu	1880	3	were	be	VBD
cord-014597-66vd2mdu	1880	4	incubated	incubate	VBN
cord-014597-66vd2mdu	1880	5	in	in	IN
cord-014597-66vd2mdu	1880	6	a	a	DT
cord-014597-66vd2mdu	1880	7	Climo	Climo	NNP
cord-014597-66vd2mdu	1880	8	-	-	HYPH
cord-014597-66vd2mdu	1880	9	shaker	shaker	NN
cord-014597-66vd2mdu	1880	10	ISF1-X	ISF1-X	NNP
cord-014597-66vd2mdu	1880	11	(	(	-LRB-
cord-014597-66vd2mdu	1880	12	Kuhner	Kuhner	NNP
cord-014597-66vd2mdu	1880	13	)	)	-RRB-
cord-014597-66vd2mdu	1880	14	.	.	.
cord-014597-66vd2mdu	1881	1	Antibody	antibody	NN
cord-014597-66vd2mdu	1881	2	production	production	NN
cord-014597-66vd2mdu	1881	3	was	be	VBD
cord-014597-66vd2mdu	1881	4	measured	measure	VBN
cord-014597-66vd2mdu	1881	5	using	use	VBG
cord-014597-66vd2mdu	1881	6	biolayer	biolayer	NN
cord-014597-66vd2mdu	1881	7	interferometry	interferometry	NN
cord-014597-66vd2mdu	1881	8	with	with	IN
cord-014597-66vd2mdu	1881	9	an	an	DT
cord-014597-66vd2mdu	1881	10	Octet	octet	NN
cord-014597-66vd2mdu	1881	11	QK	QK	NNP
cord-014597-66vd2mdu	1881	12	system	system	NN
cord-014597-66vd2mdu	1881	13	(	(	-LRB-
cord-014597-66vd2mdu	1881	14	Fortebio	Fortebio	NNP
cord-014597-66vd2mdu	1881	15	)	)	-RRB-
cord-014597-66vd2mdu	1881	16	.	.	.
cord-014597-66vd2mdu	1882	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1882	2	constructed	construct	VBD
cord-014597-66vd2mdu	1882	3	four	four	CD
cord-014597-66vd2mdu	1882	4	CHO	CHO	NNP
cord-014597-66vd2mdu	1882	5	cell	cell	NN
cord-014597-66vd2mdu	1882	6	lines	line	NNS
cord-014597-66vd2mdu	1882	7	stably	stably	RB
cord-014597-66vd2mdu	1882	8	expressing	express	VBG
cord-014597-66vd2mdu	1882	9	EBNA1	EBNA1	NNS
cord-014597-66vd2mdu	1882	10	,	,	,
cord-014597-66vd2mdu	1882	11	termed	term	VBD
cord-014597-66vd2mdu	1882	12	IgG1-EB01	IgG1-EB01	NNPS
cord-014597-66vd2mdu	1882	13	to	to	IN
cord-014597-66vd2mdu	1882	14	EB04	EB04	NNP
cord-014597-66vd2mdu	1882	15	.	.	.
cord-014597-66vd2mdu	1883	1	In	in	IN
cord-014597-66vd2mdu	1883	2	capillary	capillary	JJ
cord-014597-66vd2mdu	1883	3	electrophoresis	electrophoresis	NN
cord-014597-66vd2mdu	1883	4	analysis	analysis	NN
cord-014597-66vd2mdu	1883	5	,	,	,
cord-014597-66vd2mdu	1883	6	we	-PRON-	PRP
cord-014597-66vd2mdu	1883	7	observed	observe	VBD
cord-014597-66vd2mdu	1883	8	a	a	DT
cord-014597-66vd2mdu	1883	9	clear	clear	JJ
cord-014597-66vd2mdu	1883	10	peak	peak	NN
cord-014597-66vd2mdu	1883	11	corresponding	correspond	VBG
cord-014597-66vd2mdu	1883	12	to	to	IN
cord-014597-66vd2mdu	1883	13	the	the	DT
cord-014597-66vd2mdu	1883	14	EBNA1	EBNA1	NNS
cord-014597-66vd2mdu	1883	15	expression	expression	NN
cord-014597-66vd2mdu	1883	16	in	in	IN
cord-014597-66vd2mdu	1883	17	all	all	DT
cord-014597-66vd2mdu	1883	18	four	four	CD
cord-014597-66vd2mdu	1883	19	cell	cell	NN
cord-014597-66vd2mdu	1883	20	lines	line	NNS
cord-014597-66vd2mdu	1883	21	.	.	.
cord-014597-66vd2mdu	1884	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1884	2	tested	test	VBD
cord-014597-66vd2mdu	1884	3	the	the	DT
cord-014597-66vd2mdu	1884	4	transfection	transfection	NN
cord-014597-66vd2mdu	1884	5	efficiency	efficiency	NN
cord-014597-66vd2mdu	1884	6	by	by	IN
cord-014597-66vd2mdu	1884	7	pOTC	pOTC	NNS
cord-014597-66vd2mdu	1884	8	-	-	HYPH
cord-014597-66vd2mdu	1884	9	GFP	gfp	NN
cord-014597-66vd2mdu	1884	10	plasmids	plasmid	NNS
cord-014597-66vd2mdu	1884	11	.	.	.
cord-014597-66vd2mdu	1885	1	In	in	IN
cord-014597-66vd2mdu	1885	2	the	the	DT
cord-014597-66vd2mdu	1885	3	best	good	JJS
cord-014597-66vd2mdu	1885	4	transfection	transfection	NN
cord-014597-66vd2mdu	1885	5	condition	condition	NN
cord-014597-66vd2mdu	1885	6	,	,	,
cord-014597-66vd2mdu	1885	7	PEI	PEI	NNP
cord-014597-66vd2mdu	1885	8	/	/	SYM
cord-014597-66vd2mdu	1885	9	DNA	dna	NN
cord-014597-66vd2mdu	1885	10	ratio	ratio	NN
cord-014597-66vd2mdu	1885	11	of	of	IN
cord-014597-66vd2mdu	1885	12	1/1	1/1	CD
cord-014597-66vd2mdu	1885	13	,	,	,
cord-014597-66vd2mdu	1885	14	IgG1-EB01	IgG1-EB01	NNP
cord-014597-66vd2mdu	1885	15	cell	cell	NN
cord-014597-66vd2mdu	1885	16	showed	show	VBD
cord-014597-66vd2mdu	1885	17	the	the	DT
cord-014597-66vd2mdu	1885	18	highest	high	JJS
cord-014597-66vd2mdu	1885	19	GFP	gfp	NN
cord-014597-66vd2mdu	1885	20	-	-	HYPH
cord-014597-66vd2mdu	1885	21	positive	positive	JJ
cord-014597-66vd2mdu	1885	22	cell	cell	NN
cord-014597-66vd2mdu	1885	23	number	number	NN
cord-014597-66vd2mdu	1885	24	(	(	-LRB-
cord-014597-66vd2mdu	1885	25	1.07×10	1.07×10	CD
cord-014597-66vd2mdu	1885	26	7	7	CD
cord-014597-66vd2mdu	1885	27	cell	cell	NN
cord-014597-66vd2mdu	1885	28	/	/	SYM
cord-014597-66vd2mdu	1885	29	mL	ml	NN
cord-014597-66vd2mdu	1885	30	)	)	-RRB-
cord-014597-66vd2mdu	1885	31	and	and	CC
cord-014597-66vd2mdu	1885	32	transfection	transfection	NN
cord-014597-66vd2mdu	1885	33	efficiency	efficiency	NN
cord-014597-66vd2mdu	1885	34	(	(	-LRB-
cord-014597-66vd2mdu	1885	35	95	95	CD
cord-014597-66vd2mdu	1885	36	%	%	NN
cord-014597-66vd2mdu	1885	37	)	)	-RRB-
cord-014597-66vd2mdu	1885	38	among	among	IN
cord-014597-66vd2mdu	1885	39	the	the	DT
cord-014597-66vd2mdu	1885	40	four	four	CD
cord-014597-66vd2mdu	1885	41	cell	cell	NN
cord-014597-66vd2mdu	1885	42	lines	line	NNS
cord-014597-66vd2mdu	1885	43	.	.	.
cord-014597-66vd2mdu	1886	1	Therefore	therefore	RB
cord-014597-66vd2mdu	1886	2	,	,	,
cord-014597-66vd2mdu	1886	3	IgG1-EB01	IgG1-EB01	NNP
cord-014597-66vd2mdu	1886	4	cell	cell	NN
cord-014597-66vd2mdu	1886	5	lines	line	NNS
cord-014597-66vd2mdu	1886	6	were	be	VBD
cord-014597-66vd2mdu	1886	7	selected	select	VBN
cord-014597-66vd2mdu	1886	8	for	for	IN
cord-014597-66vd2mdu	1886	9	further	further	JJ
cord-014597-66vd2mdu	1886	10	study	study	NN
cord-014597-66vd2mdu	1886	11	.	.	.
cord-014597-66vd2mdu	1887	1	After	after	IN
cord-014597-66vd2mdu	1887	2	the	the	DT
cord-014597-66vd2mdu	1887	3	transfection	transfection	NN
cord-014597-66vd2mdu	1887	4	,	,	,
cord-014597-66vd2mdu	1887	5	the	the	DT
cord-014597-66vd2mdu	1887	6	number	number	NN
cord-014597-66vd2mdu	1887	7	of	of	IN
cord-014597-66vd2mdu	1887	8	GFP	gfp	NN
cord-014597-66vd2mdu	1887	9	-	-	HYPH
cord-014597-66vd2mdu	1887	10	positive	positive	JJ
cord-014597-66vd2mdu	1887	11	cells	cell	NNS
cord-014597-66vd2mdu	1887	12	continued	continue	VBD
cord-014597-66vd2mdu	1887	13	to	to	TO
cord-014597-66vd2mdu	1887	14	increase	increase	VB
cord-014597-66vd2mdu	1887	15	even	even	RB
cord-014597-66vd2mdu	1887	16	after	after	IN
cord-014597-66vd2mdu	1887	17	the	the	DT
cord-014597-66vd2mdu	1887	18	passage	passage	NN
cord-014597-66vd2mdu	1887	19	(	(	-LRB-
cord-014597-66vd2mdu	1887	20	Fig	Fig	NNP
cord-014597-66vd2mdu	1887	21	.	.	.
cord-014597-66vd2mdu	1887	22	1	1	CD
cord-014597-66vd2mdu	1887	23	)	)	-RRB-
cord-014597-66vd2mdu	1887	24	,	,	,
cord-014597-66vd2mdu	1887	25	suggesting	suggest	VBG
cord-014597-66vd2mdu	1887	26	that	that	IN
cord-014597-66vd2mdu	1887	27	the	the	DT
cord-014597-66vd2mdu	1887	28	pOTC	potc	CD
cord-014597-66vd2mdu	1887	29	-	-	HYPH
cord-014597-66vd2mdu	1887	30	GFP	gfp	NN
cord-014597-66vd2mdu	1887	31	plasmid	plasmid	NN
cord-014597-66vd2mdu	1887	32	was	be	VBD
cord-014597-66vd2mdu	1887	33	stably	stably	RB
cord-014597-66vd2mdu	1887	34	retained	retain	VBN
cord-014597-66vd2mdu	1887	35	and	and	CC
cord-014597-66vd2mdu	1887	36	replicated	replicate	VBN
cord-014597-66vd2mdu	1887	37	by	by	IN
cord-014597-66vd2mdu	1887	38	EBNA1	EBNA1	NNP
cord-014597-66vd2mdu	1887	39	/	/	SYM
cord-014597-66vd2mdu	1887	40	oriP	orip	CD
cord-014597-66vd2mdu	1887	41	system	system	NN
cord-014597-66vd2mdu	1887	42	in	in	IN
cord-014597-66vd2mdu	1887	43	IgG1-EB01	IgG1-EB01	NNP
cord-014597-66vd2mdu	1887	44	cell	cell	NN
cord-014597-66vd2mdu	1887	45	lines	line	NNS
cord-014597-66vd2mdu	1887	46	.	.	.
cord-014597-66vd2mdu	1888	1	In	in	IN
cord-014597-66vd2mdu	1888	2	preliminary	preliminary	JJ
cord-014597-66vd2mdu	1888	3	experiments	experiment	NNS
cord-014597-66vd2mdu	1888	4	,	,	,
cord-014597-66vd2mdu	1888	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1888	6	introduced	introduce	VBD
cord-014597-66vd2mdu	1888	7	three	three	CD
cord-014597-66vd2mdu	1888	8	genes	gene	NNS
cord-014597-66vd2mdu	1888	9	,	,	,
cord-014597-66vd2mdu	1888	10	MDH2	MDH2	NNP
cord-014597-66vd2mdu	1888	11	,	,	,
cord-014597-66vd2mdu	1888	12	GSS	GSS	NNP
cord-014597-66vd2mdu	1888	13	and	and	CC
cord-014597-66vd2mdu	1888	14	GCLM	GCLM	NNP
cord-014597-66vd2mdu	1888	15	,	,	,
cord-014597-66vd2mdu	1888	16	into	into	IN
cord-014597-66vd2mdu	1888	17	IgG1-EB01	IgG1-EB01	NNP
cord-014597-66vd2mdu	1888	18	cell	cell	NN
cord-014597-66vd2mdu	1888	19	lines	line	NNS
cord-014597-66vd2mdu	1888	20	.	.	.
cord-014597-66vd2mdu	1889	1	Cotransfection	cotransfection	NN
cord-014597-66vd2mdu	1889	2	of	of	IN
cord-014597-66vd2mdu	1889	3	these	these	DT
cord-014597-66vd2mdu	1889	4	three	three	CD
cord-014597-66vd2mdu	1889	5	genes	gene	NNS
cord-014597-66vd2mdu	1889	6	led	lead	VBD
cord-014597-66vd2mdu	1889	7	to	to	IN
cord-014597-66vd2mdu	1889	8	an	an	DT
cord-014597-66vd2mdu	1889	9	increase	increase	NN
cord-014597-66vd2mdu	1889	10	in	in	IN
cord-014597-66vd2mdu	1889	11	IgG1	igg1	CD
cord-014597-66vd2mdu	1889	12	production	production	NN
cord-014597-66vd2mdu	1889	13	from	from	IN
cord-014597-66vd2mdu	1889	14	287±18	287±18	CD
cord-014597-66vd2mdu	1889	15	mg	mg	NNP
cord-014597-66vd2mdu	1889	16	/	/	SYM
cord-014597-66vd2mdu	1889	17	L	L	NNP
cord-014597-66vd2mdu	1889	18	(	(	-LRB-
cord-014597-66vd2mdu	1889	19	control	control	NN
cord-014597-66vd2mdu	1889	20	)	)	-RRB-
cord-014597-66vd2mdu	1889	21	to	to	IN
cord-014597-66vd2mdu	1889	22	334±21	334±21	CD
cord-014597-66vd2mdu	1889	23	mg	mg	NNP
cord-014597-66vd2mdu	1889	24	/	/	SYM
cord-014597-66vd2mdu	1889	25	L	L	NNP
cord-014597-66vd2mdu	1889	26	at	at	IN
cord-014597-66vd2mdu	1889	27	day	day	NN
cord-014597-66vd2mdu	1889	28	8	8	CD
cord-014597-66vd2mdu	1889	29	(	(	-LRB-
cord-014597-66vd2mdu	1889	30	P<0.05	P<0.05	NNS
cord-014597-66vd2mdu	1889	31	,	,	,
cord-014597-66vd2mdu	1889	32	t	t	NN
cord-014597-66vd2mdu	1889	33	-	-	HYPH
cord-014597-66vd2mdu	1889	34	test	test	NN
cord-014597-66vd2mdu	1889	35	,	,	,
cord-014597-66vd2mdu	1889	36	n=3	n=3	NN
cord-014597-66vd2mdu	1889	37	)	)	-RRB-
cord-014597-66vd2mdu	1889	38	.	.	.
cord-014597-66vd2mdu	1890	1	This	this	DT
cord-014597-66vd2mdu	1890	2	result	result	NN
cord-014597-66vd2mdu	1890	3	suggests	suggest	VBZ
cord-014597-66vd2mdu	1890	4	that	that	IN
cord-014597-66vd2mdu	1890	5	these	these	DT
cord-014597-66vd2mdu	1890	6	three	three	CD
cord-014597-66vd2mdu	1890	7	genes	gene	NNS
cord-014597-66vd2mdu	1890	8	work	work	VBP
cord-014597-66vd2mdu	1890	9	as	as	IN
cord-014597-66vd2mdu	1890	10	production	production	NN
cord-014597-66vd2mdu	1890	11	enhancer	enhancer	NN
cord-014597-66vd2mdu	1890	12	genes	gene	NNS
cord-014597-66vd2mdu	1890	13	.	.	.
cord-014597-66vd2mdu	1891	1	Conventional	conventional	JJ
cord-014597-66vd2mdu	1891	2	methods	method	NNS
cord-014597-66vd2mdu	1891	3	based	base	VBN
cord-014597-66vd2mdu	1891	4	on	on	IN
cord-014597-66vd2mdu	1891	5	stable	stable	JJ
cord-014597-66vd2mdu	1891	6	cells	cell	NNS
cord-014597-66vd2mdu	1891	7	take	take	VBP
cord-014597-66vd2mdu	1891	8	up	up	IN
cord-014597-66vd2mdu	1891	9	to	to	TO
cord-014597-66vd2mdu	1891	10	6	6	CD
cord-014597-66vd2mdu	1891	11	months	month	NNS
cord-014597-66vd2mdu	1891	12	to	to	TO
cord-014597-66vd2mdu	1891	13	determine	determine	VB
cord-014597-66vd2mdu	1891	14	whether	whether	IN
cord-014597-66vd2mdu	1891	15	the	the	DT
cord-014597-66vd2mdu	1891	16	gene	gene	NN
cord-014597-66vd2mdu	1891	17	of	of	IN
cord-014597-66vd2mdu	1891	18	interest	interest	NN
cord-014597-66vd2mdu	1891	19	is	be	VBZ
cord-014597-66vd2mdu	1891	20	beneficial	beneficial	JJ
cord-014597-66vd2mdu	1891	21	for	for	IN
cord-014597-66vd2mdu	1891	22	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1891	23	IgG1	igg1	CD
cord-014597-66vd2mdu	1891	24	production	production	NN
cord-014597-66vd2mdu	1891	25	.	.	.
cord-014597-66vd2mdu	1892	1	In	in	IN
cord-014597-66vd2mdu	1892	2	contrast	contrast	NN
cord-014597-66vd2mdu	1892	3	,	,	,
cord-014597-66vd2mdu	1892	4	identification	identification	NN
cord-014597-66vd2mdu	1892	5	of	of	IN
cord-014597-66vd2mdu	1892	6	production	production	NN
cord-014597-66vd2mdu	1892	7	enhancer	enhancer	NN
cord-014597-66vd2mdu	1892	8	genes	gene	NNS
cord-014597-66vd2mdu	1892	9	is	be	VBZ
cord-014597-66vd2mdu	1892	10	achievable	achievable	JJ
cord-014597-66vd2mdu	1892	11	within	within	IN
cord-014597-66vd2mdu	1892	12	10	10	CD
cord-014597-66vd2mdu	1892	13	days	day	NNS
cord-014597-66vd2mdu	1892	14	by	by	IN
cord-014597-66vd2mdu	1892	15	our	-PRON-	PRP$
cord-014597-66vd2mdu	1892	16	proposed	propose	VBN
cord-014597-66vd2mdu	1892	17	method	method	NN
cord-014597-66vd2mdu	1892	18	based	base	VBN
cord-014597-66vd2mdu	1892	19	on	on	IN
cord-014597-66vd2mdu	1892	20	EBNA1	EBNA1	NNS
cord-014597-66vd2mdu	1892	21	/	/	SYM
cord-014597-66vd2mdu	1892	22	oriP	orip	CD
cord-014597-66vd2mdu	1892	23	system	system	NN
cord-014597-66vd2mdu	1892	24	.	.	.
cord-014597-66vd2mdu	1893	1	The	the	DT
cord-014597-66vd2mdu	1893	2	proposed	propose	VBN
cord-014597-66vd2mdu	1893	3	method	method	NN
cord-014597-66vd2mdu	1893	4	makes	make	VBZ
cord-014597-66vd2mdu	1893	5	it	-PRON-	PRP
cord-014597-66vd2mdu	1893	6	possible	possible	JJ
cord-014597-66vd2mdu	1893	7	to	to	TO
cord-014597-66vd2mdu	1893	8	evaluate	evaluate	VB
cord-014597-66vd2mdu	1893	9	production	production	NN
cord-014597-66vd2mdu	1893	10	enhancer	enhancer	NN
cord-014597-66vd2mdu	1893	11	genes	gene	NNS
cord-014597-66vd2mdu	1893	12	in	in	IN
cord-014597-66vd2mdu	1893	13	a	a	DT
cord-014597-66vd2mdu	1893	14	rapid	rapid	JJ
cord-014597-66vd2mdu	1893	15	manner	manner	NN
cord-014597-66vd2mdu	1893	16	.	.	.
cord-014597-66vd2mdu	1894	1	The	the	DT
cord-014597-66vd2mdu	1894	2	proposed	propose	VBN
cord-014597-66vd2mdu	1894	3	method	method	NN
cord-014597-66vd2mdu	1894	4	is	be	VBZ
cord-014597-66vd2mdu	1894	5	a	a	DT
cord-014597-66vd2mdu	1894	6	promising	promising	JJ
cord-014597-66vd2mdu	1894	7	approach	approach	NN
cord-014597-66vd2mdu	1894	8	to	to	TO
cord-014597-66vd2mdu	1894	9	identify	identify	VB
cord-014597-66vd2mdu	1894	10	genes	gene	NNS
cord-014597-66vd2mdu	1894	11	enhancing	enhance	VBG
cord-014597-66vd2mdu	1894	12	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1894	13	antibody	antibody	NN
cord-014597-66vd2mdu	1894	14	production	production	NN
cord-014597-66vd2mdu	1894	15	.	.	.
cord-014597-66vd2mdu	1895	1	Background	background	NN
cord-014597-66vd2mdu	1895	2	2	2	CD
cord-014597-66vd2mdu	1895	3	G	G	NNP
cord-014597-66vd2mdu	1895	4	UNic	UNic	NNP
cord-014597-66vd2mdu	1895	5	™	™	NN
cord-014597-66vd2mdu	1895	6	(	(	-LRB-
cord-014597-66vd2mdu	1895	7	2GUN	2gun	LS
cord-014597-66vd2mdu	1895	8	)	)	-RRB-
cord-014597-66vd2mdu	1895	9	technology	technology	NN
cord-014597-66vd2mdu	1895	10	comprises	comprise	VBZ
cord-014597-66vd2mdu	1895	11	a	a	DT
cord-014597-66vd2mdu	1895	12	set	set	NN
cord-014597-66vd2mdu	1895	13	of	of	IN
cord-014597-66vd2mdu	1895	14	protected	protect	VBN
cord-014597-66vd2mdu	1895	15	genetic	genetic	JJ
cord-014597-66vd2mdu	1895	16	elements	element	NNS
cord-014597-66vd2mdu	1895	17	that	that	WDT
cord-014597-66vd2mdu	1895	18	improve	improve	VBP
cord-014597-66vd2mdu	1895	19	protein	protein	NN
cord-014597-66vd2mdu	1895	20	production	production	NN
cord-014597-66vd2mdu	1895	21	by	by	IN
cord-014597-66vd2mdu	1895	22	acting	act	VBG
cord-014597-66vd2mdu	1895	23	on	on	IN
cord-014597-66vd2mdu	1895	24	transcription	transcription	NN
cord-014597-66vd2mdu	1895	25	as	as	RB
cord-014597-66vd2mdu	1895	26	well	well	RB
cord-014597-66vd2mdu	1895	27	as	as	IN
cord-014597-66vd2mdu	1895	28	on	on	IN
cord-014597-66vd2mdu	1895	29	translation	translation	NN
cord-014597-66vd2mdu	1895	30	.	.	.
cord-014597-66vd2mdu	1896	1	The	the	DT
cord-014597-66vd2mdu	1896	2	elements	element	NNS
cord-014597-66vd2mdu	1896	3	can	can	MD
cord-014597-66vd2mdu	1896	4	either	either	CC
cord-014597-66vd2mdu	1896	5	be	be	VB
cord-014597-66vd2mdu	1896	6	inserted	insert	VBN
cord-014597-66vd2mdu	1896	7	into	into	IN
cord-014597-66vd2mdu	1896	8	existing	exist	VBG
cord-014597-66vd2mdu	1896	9	(	(	-LRB-
cord-014597-66vd2mdu	1896	10	platform	platform	NN
cord-014597-66vd2mdu	1896	11	)	)	-RRB-
cord-014597-66vd2mdu	1896	12	vectors	vector	NNS
cord-014597-66vd2mdu	1896	13	or	or	CC
cord-014597-66vd2mdu	1896	14	be	be	VB
cord-014597-66vd2mdu	1896	15	provided	provide	VBN
cord-014597-66vd2mdu	1896	16	as	as	IN
cord-014597-66vd2mdu	1896	17	complete	complete	JJ
cord-014597-66vd2mdu	1896	18	ready	ready	JJ
cord-014597-66vd2mdu	1896	19	-	-	HYPH
cord-014597-66vd2mdu	1896	20	to	to	IN
cord-014597-66vd2mdu	1896	21	-	-	HYPH
cord-014597-66vd2mdu	1896	22	use	use	NN
cord-014597-66vd2mdu	1896	23	vectors	vector	NNS
cord-014597-66vd2mdu	1896	24	.	.	.
cord-014597-66vd2mdu	1897	1	The	the	DT
cord-014597-66vd2mdu	1897	2	technology	technology	NN
cord-014597-66vd2mdu	1897	3	can	can	MD
cord-014597-66vd2mdu	1897	4	be	be	VB
cord-014597-66vd2mdu	1897	5	used	use	VBN
cord-014597-66vd2mdu	1897	6	in	in	IN
cord-014597-66vd2mdu	1897	7	stable	stable	JJ
cord-014597-66vd2mdu	1897	8	and	and	CC
cord-014597-66vd2mdu	1897	9	in	in	IN
cord-014597-66vd2mdu	1897	10	transient	transient	JJ
cord-014597-66vd2mdu	1897	11	transfection	transfection	NN
cord-014597-66vd2mdu	1897	12	to	to	TO
cord-014597-66vd2mdu	1897	13	boost	boost	VB
cord-014597-66vd2mdu	1897	14	protein	protein	NN
cord-014597-66vd2mdu	1897	15	production	production	NN
cord-014597-66vd2mdu	1897	16	for	for	IN
cord-014597-66vd2mdu	1897	17	product	product	NN
cord-014597-66vd2mdu	1897	18	development	development	NN
cord-014597-66vd2mdu	1897	19	and	and	CC
cord-014597-66vd2mdu	1897	20	is	be	VBZ
cord-014597-66vd2mdu	1897	21	being	be	VBG
cord-014597-66vd2mdu	1897	22	applied	apply	VBN
cord-014597-66vd2mdu	1897	23	in	in	IN
cord-014597-66vd2mdu	1897	24	CLD	CLD	NNP
cord-014597-66vd2mdu	1897	25	for	for	IN
cord-014597-66vd2mdu	1897	26	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	1897	27	proteins	protein	NNS
cord-014597-66vd2mdu	1897	28	.	.	.
cord-014597-66vd2mdu	1898	1	In	in	IN
cord-014597-66vd2mdu	1898	2	combination	combination	NN
cord-014597-66vd2mdu	1898	3	with	with	IN
cord-014597-66vd2mdu	1898	4	antibiotic	antibiotic	JJ
cord-014597-66vd2mdu	1898	5	selection	selection	NN
cord-014597-66vd2mdu	1898	6	or	or	CC
cord-014597-66vd2mdu	1898	7	DHFR	DHFR	NNP
cord-014597-66vd2mdu	1898	8	selection	selection	NN
cord-014597-66vd2mdu	1898	9	,	,	,
cord-014597-66vd2mdu	1898	10	2GUN	2GUN	NNP
cord-014597-66vd2mdu	1898	11	technology	technology	NN
cord-014597-66vd2mdu	1898	12	routinely	routinely	RB
cord-014597-66vd2mdu	1898	13	results	result	VBZ
cord-014597-66vd2mdu	1898	14	in	in	IN
cord-014597-66vd2mdu	1898	15	2	2	CD
cord-014597-66vd2mdu	1898	16	-	-	SYM
cord-014597-66vd2mdu	1898	17	3	3	CD
cord-014597-66vd2mdu	1898	18	fold	fold	VB
cord-014597-66vd2mdu	1898	19	increase	increase	NN
cord-014597-66vd2mdu	1898	20	in	in	IN
cord-014597-66vd2mdu	1898	21	expression	expression	NN
cord-014597-66vd2mdu	1898	22	of	of	IN
cord-014597-66vd2mdu	1898	23	client	client	NN
cord-014597-66vd2mdu	1898	24	antibodies	antibody	NNS
cord-014597-66vd2mdu	1898	25	or	or	CC
cord-014597-66vd2mdu	1898	26	fusion	fusion	NN
cord-014597-66vd2mdu	1898	27	proteins	protein	NNS
cord-014597-66vd2mdu	1898	28	,	,	,
cord-014597-66vd2mdu	1898	29	both	both	CC
cord-014597-66vd2mdu	1898	30	in	in	IN
cord-014597-66vd2mdu	1898	31	pools	pool	NNS
cord-014597-66vd2mdu	1898	32	and	and	CC
cord-014597-66vd2mdu	1898	33	after	after	IN
cord-014597-66vd2mdu	1898	34	clonal	clonal	JJ
cord-014597-66vd2mdu	1898	35	selection	selection	NN
cord-014597-66vd2mdu	1898	36	.	.	.
cord-014597-66vd2mdu	1899	1	Previously	previously	RB
cord-014597-66vd2mdu	1899	2	,	,	,
cord-014597-66vd2mdu	1899	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1899	4	have	have	VBP
cord-014597-66vd2mdu	1899	5	successfully	successfully	RB
cord-014597-66vd2mdu	1899	6	combined	combine	VBN
cord-014597-66vd2mdu	1899	7	2GUN	2gun	CD
cord-014597-66vd2mdu	1899	8	technology	technology	NN
cord-014597-66vd2mdu	1899	9	with	with	IN
cord-014597-66vd2mdu	1899	10	glutamine	glutamine	NN
cord-014597-66vd2mdu	1899	11	synthetase	synthetase	NN
cord-014597-66vd2mdu	1899	12	(	(	-LRB-
cord-014597-66vd2mdu	1899	13	GS	GS	NNP
cord-014597-66vd2mdu	1899	14	)	)	-RRB-
cord-014597-66vd2mdu	1899	15	selection	selection	NN
cord-014597-66vd2mdu	1899	16	and	and	CC
cord-014597-66vd2mdu	1899	17	the	the	DT
cord-014597-66vd2mdu	1899	18	CHO	CHO	NNP
cord-014597-66vd2mdu	1899	19	GS	GS	NNP
cord-014597-66vd2mdu	1899	20	null	null	NN
cord-014597-66vd2mdu	1899	21	cells	cell	NNS
cord-014597-66vd2mdu	1899	22	of	of	IN
cord-014597-66vd2mdu	1899	23	Horizon	Horizon	NNP
cord-014597-66vd2mdu	1899	24	Discovery	Discovery	NNP
cord-014597-66vd2mdu	1899	25	,	,	,
cord-014597-66vd2mdu	1899	26	resulting	result	VBG
cord-014597-66vd2mdu	1899	27	in	in	IN
cord-014597-66vd2mdu	1899	28	clonal	clonal	JJ
cord-014597-66vd2mdu	1899	29	cell	cell	NN
cord-014597-66vd2mdu	1899	30	lines	line	NNS
cord-014597-66vd2mdu	1899	31	producing	produce	VBG
cord-014597-66vd2mdu	1899	32	>	>	XX
cord-014597-66vd2mdu	1900	1	6	6	CD
cord-014597-66vd2mdu	1900	2	g	g	NN
cord-014597-66vd2mdu	1900	3	/	/	SYM
cord-014597-66vd2mdu	1900	4	L	l	NN
cord-014597-66vd2mdu	1900	5	of	of	IN
cord-014597-66vd2mdu	1900	6	a	a	DT
cord-014597-66vd2mdu	1900	7	biosimilar	biosimilar	NNP
cord-014597-66vd2mdu	1900	8	mAb	mab	NN
cord-014597-66vd2mdu	1900	9	in	in	IN
cord-014597-66vd2mdu	1900	10	fed	fed	NNP
cord-014597-66vd2mdu	1900	11	-	-	HYPH
cord-014597-66vd2mdu	1900	12	batch	batch	NN
cord-014597-66vd2mdu	1900	13	assay	assay	NN
cord-014597-66vd2mdu	1900	14	.	.	.
cord-014597-66vd2mdu	1901	1	Here	here	RB
cord-014597-66vd2mdu	1901	2	we	-PRON-	PRP
cord-014597-66vd2mdu	1901	3	present	present	VBP
cord-014597-66vd2mdu	1901	4	data	datum	NNS
cord-014597-66vd2mdu	1901	5	on	on	IN
cord-014597-66vd2mdu	1901	6	the	the	DT
cord-014597-66vd2mdu	1901	7	successful	successful	JJ
cord-014597-66vd2mdu	1901	8	application	application	NN
cord-014597-66vd2mdu	1901	9	of	of	IN
cord-014597-66vd2mdu	1901	10	the	the	DT
cord-014597-66vd2mdu	1901	11	2GUN	2GUN	NNP
cord-014597-66vd2mdu	1901	12	technology	technology	NN
cord-014597-66vd2mdu	1901	13	for	for	IN
cord-014597-66vd2mdu	1901	14	the	the	DT
cord-014597-66vd2mdu	1901	15	enhanced	enhanced	JJ
cord-014597-66vd2mdu	1901	16	expression	expression	NN
cord-014597-66vd2mdu	1901	17	of	of	IN
cord-014597-66vd2mdu	1901	18	a	a	DT
cord-014597-66vd2mdu	1901	19	large	large	JJ
cord-014597-66vd2mdu	1901	20	(	(	-LRB-
cord-014597-66vd2mdu	1901	21	>	>	NFP
cord-014597-66vd2mdu	1901	22	300	300	CD
cord-014597-66vd2mdu	1901	23	kDa	kDa	NNPS
cord-014597-66vd2mdu	1901	24	)	)	-RRB-
cord-014597-66vd2mdu	1901	25	human	human	JJ
cord-014597-66vd2mdu	1901	26	heterotrimeric	heterotrimeric	NNP
cord-014597-66vd2mdu	1901	27	glycoprotein	glycoprotein	NN
cord-014597-66vd2mdu	1901	28	,	,	,
cord-014597-66vd2mdu	1901	29	a	a	DT
cord-014597-66vd2mdu	1901	30	renowned	renowned	JJ
cord-014597-66vd2mdu	1901	31	difficult	difficult	JJ
cord-014597-66vd2mdu	1901	32	-	-	HYPH
cord-014597-66vd2mdu	1901	33	to	to	TO
cord-014597-66vd2mdu	1901	34	-	-	HYPH
cord-014597-66vd2mdu	1901	35	express	express	VB
cord-014597-66vd2mdu	1901	36	(	(	-LRB-
cord-014597-66vd2mdu	1901	37	DTE	DTE	NNP
cord-014597-66vd2mdu	1901	38	)	)	-RRB-
cord-014597-66vd2mdu	1901	39	protein	protein	NN
cord-014597-66vd2mdu	1901	40	.	.	.
cord-014597-66vd2mdu	1902	1	All	all	DT
cord-014597-66vd2mdu	1902	2	expression	expression	NN
cord-014597-66vd2mdu	1902	3	vectors	vector	NNS
cord-014597-66vd2mdu	1902	4	comprised	comprise	VBD
cord-014597-66vd2mdu	1902	5	a	a	DT
cord-014597-66vd2mdu	1902	6	hCMV	hcmv	NN
cord-014597-66vd2mdu	1902	7	promoter	promoter	NN
cord-014597-66vd2mdu	1902	8	and	and	CC
cord-014597-66vd2mdu	1902	9	BGH	BGH	NNP
cord-014597-66vd2mdu	1902	10	polyadenylation	polyadenylation	NN
cord-014597-66vd2mdu	1902	11	sequence	sequence	NN
cord-014597-66vd2mdu	1902	12	in	in	IN
cord-014597-66vd2mdu	1902	13	the	the	DT
cord-014597-66vd2mdu	1902	14	expression	expression	NN
cord-014597-66vd2mdu	1902	15	cassettes	cassette	NNS
cord-014597-66vd2mdu	1902	16	for	for	IN
cord-014597-66vd2mdu	1902	17	the	the	DT
cord-014597-66vd2mdu	1902	18	gene	gene	NN
cord-014597-66vd2mdu	1902	19	of	of	IN
cord-014597-66vd2mdu	1902	20	interest	interest	NN
cord-014597-66vd2mdu	1902	21	,	,	,
cord-014597-66vd2mdu	1902	22	and	and	CC
cord-014597-66vd2mdu	1902	23	a	a	DT
cord-014597-66vd2mdu	1902	24	selection	selection	NN
cord-014597-66vd2mdu	1902	25	marker	marker	NN
cord-014597-66vd2mdu	1902	26	gene	gene	NN
cord-014597-66vd2mdu	1902	27	with	with	IN
cord-014597-66vd2mdu	1902	28	SV40	SV40	NNP
cord-014597-66vd2mdu	1902	29	promoter	promoter	NN
cord-014597-66vd2mdu	1902	30	and	and	CC
cord-014597-66vd2mdu	1902	31	SV40	SV40	NNP
cord-014597-66vd2mdu	1902	32	polyadenylation	polyadenylation	NN
cord-014597-66vd2mdu	1902	33	sequence	sequence	NN
cord-014597-66vd2mdu	1902	34	.	.	.
cord-014597-66vd2mdu	1903	1	2	2	LS
cord-014597-66vd2mdu	1903	2	G	G	NNP
cord-014597-66vd2mdu	1903	3	UNic	UNic	NNP
cord-014597-66vd2mdu	1903	4	™	™	NN
cord-014597-66vd2mdu	1903	5	Vectors	Vectors	NNP
cord-014597-66vd2mdu	1903	6	also	also	RB
cord-014597-66vd2mdu	1903	7	contained	contain	VBD
cord-014597-66vd2mdu	1903	8	genetic	genetic	JJ
cord-014597-66vd2mdu	1903	9	elements	element	NNS
cord-014597-66vd2mdu	1903	10	(	(	-LRB-
cord-014597-66vd2mdu	1903	11	2	2	CD
cord-014597-66vd2mdu	1903	12	G	G	NNP
cord-014597-66vd2mdu	1903	13	UNic	UNic	NNP
cord-014597-66vd2mdu	1903	14	™	™	NN
cord-014597-66vd2mdu	1903	15	technology	technology	NN
cord-014597-66vd2mdu	1903	16	,	,	,
cord-014597-66vd2mdu	1903	17	ProteoNic	ProteoNic	NNP
cord-014597-66vd2mdu	1903	18	)	)	-RRB-
cord-014597-66vd2mdu	1903	19	.	.	.
cord-014597-66vd2mdu	1904	1	CHO	CHO	NNP
cord-014597-66vd2mdu	1904	2	GS	GS	NNP
cord-014597-66vd2mdu	1904	3	null	null	NN
cord-014597-66vd2mdu	1904	4	cells	cell	NNS
cord-014597-66vd2mdu	1904	5	(	(	-LRB-
cord-014597-66vd2mdu	1904	6	Horizon	Horizon	NNP
cord-014597-66vd2mdu	1904	7	Discovery	Discovery	NNP
cord-014597-66vd2mdu	1904	8	)	)	-RRB-
cord-014597-66vd2mdu	1904	9	were	be	VBD
cord-014597-66vd2mdu	1904	10	transfected	transfecte	VBN
cord-014597-66vd2mdu	1904	11	in	in	IN
cord-014597-66vd2mdu	1904	12	duplicate	duplicate	NN
cord-014597-66vd2mdu	1904	13	with	with	IN
cord-014597-66vd2mdu	1904	14	Reference	reference	NN
cord-014597-66vd2mdu	1904	15	or	or	CC
cord-014597-66vd2mdu	1904	16	2GUN	2gun	JJ
cord-014597-66vd2mdu	1904	17	expression	expression	NN
cord-014597-66vd2mdu	1904	18	vectors	vector	NNS
cord-014597-66vd2mdu	1904	19	and	and	CC
cord-014597-66vd2mdu	1904	20	selected	select	VBN
cord-014597-66vd2mdu	1904	21	in	in	IN
cord-014597-66vd2mdu	1904	22	media	medium	NNS
cord-014597-66vd2mdu	1904	23	lacking	lack	VBG
cord-014597-66vd2mdu	1904	24	glutamine	glutamine	NN
cord-014597-66vd2mdu	1904	25	and	and	CC
cord-014597-66vd2mdu	1904	26	containing	contain	VBG
cord-014597-66vd2mdu	1904	27	the	the	DT
cord-014597-66vd2mdu	1904	28	appropriate	appropriate	JJ
cord-014597-66vd2mdu	1904	29	antibiotics	antibiotic	NNS
cord-014597-66vd2mdu	1904	30	.	.	.
cord-014597-66vd2mdu	1905	1	The	the	DT
cord-014597-66vd2mdu	1905	2	bulk	bulk	JJ
cord-014597-66vd2mdu	1905	3	pools	pool	NNS
cord-014597-66vd2mdu	1905	4	were	be	VBD
cord-014597-66vd2mdu	1905	5	seeded	seed	VBN
cord-014597-66vd2mdu	1905	6	at	at	IN
cord-014597-66vd2mdu	1905	7	equal	equal	JJ
cord-014597-66vd2mdu	1905	8	viable	viable	JJ
cord-014597-66vd2mdu	1905	9	cell	cell	NN
cord-014597-66vd2mdu	1905	10	density	density	NN
cord-014597-66vd2mdu	1905	11	after	after	IN
cord-014597-66vd2mdu	1905	12	obtaining	obtain	VBG
cord-014597-66vd2mdu	1905	13	maximum	maximum	JJ
cord-014597-66vd2mdu	1905	14	viability	viability	NN
cord-014597-66vd2mdu	1905	15	and	and	CC
cord-014597-66vd2mdu	1905	16	cultured	culture	VBN
cord-014597-66vd2mdu	1905	17	for	for	IN
cord-014597-66vd2mdu	1905	18	9	9	CD
cord-014597-66vd2mdu	1905	19	days	day	NNS
cord-014597-66vd2mdu	1905	20	without	without	IN
cord-014597-66vd2mdu	1905	21	feeding	feed	VBG
cord-014597-66vd2mdu	1905	22	(	(	-LRB-
cord-014597-66vd2mdu	1905	23	batch	batch	NN
cord-014597-66vd2mdu	1905	24	)	)	-RRB-
cord-014597-66vd2mdu	1905	25	.	.	.
cord-014597-66vd2mdu	1906	1	Expression	expression	NN
cord-014597-66vd2mdu	1906	2	of	of	IN
cord-014597-66vd2mdu	1906	3	the	the	DT
cord-014597-66vd2mdu	1906	4	target	target	NN
cord-014597-66vd2mdu	1906	5	protein	protein	NN
cord-014597-66vd2mdu	1906	6	in	in	IN
cord-014597-66vd2mdu	1906	7	cell	cell	NN
cord-014597-66vd2mdu	1906	8	culture	culture	NN
cord-014597-66vd2mdu	1906	9	supernatants	supernatant	NNS
cord-014597-66vd2mdu	1906	10	of	of	IN
cord-014597-66vd2mdu	1906	11	stable	stable	JJ
cord-014597-66vd2mdu	1906	12	bulk	bulk	JJ
cord-014597-66vd2mdu	1906	13	pools	pool	NNS
cord-014597-66vd2mdu	1906	14	was	be	VBD
cord-014597-66vd2mdu	1906	15	measured	measure	VBN
cord-014597-66vd2mdu	1906	16	by	by	IN
cord-014597-66vd2mdu	1906	17	ELISA	ELISA	NNP
cord-014597-66vd2mdu	1906	18	.	.	.
cord-014597-66vd2mdu	1907	1	The	the	DT
cord-014597-66vd2mdu	1907	2	three	three	CD
cord-014597-66vd2mdu	1907	3	protein	protein	NN
cord-014597-66vd2mdu	1907	4	subunit	subunit	NN
cord-014597-66vd2mdu	1907	5	genes	gene	NNS
cord-014597-66vd2mdu	1907	6	were	be	VBD
cord-014597-66vd2mdu	1907	7	expressed	express	VBN
cord-014597-66vd2mdu	1907	8	from	from	IN
cord-014597-66vd2mdu	1907	9	vectors	vector	NNS
cord-014597-66vd2mdu	1907	10	with	with	IN
cord-014597-66vd2mdu	1907	11	different	different	JJ
cord-014597-66vd2mdu	1907	12	selection	selection	NN
cord-014597-66vd2mdu	1907	13	markers	marker	NNS
cord-014597-66vd2mdu	1907	14	.	.	.
cord-014597-66vd2mdu	1908	1	In	in	IN
cord-014597-66vd2mdu	1908	2	the	the	DT
cord-014597-66vd2mdu	1908	3	Reference	reference	NN
cord-014597-66vd2mdu	1908	4	constructs	construct	NNS
cord-014597-66vd2mdu	1908	5	(	(	-LRB-
cord-014597-66vd2mdu	1908	6	without	without	IN
cord-014597-66vd2mdu	1908	7	2GUN	2GUN	NNP
cord-014597-66vd2mdu	1908	8	)	)	-RRB-
cord-014597-66vd2mdu	1908	9	,	,	,
cord-014597-66vd2mdu	1908	10	the	the	DT
cord-014597-66vd2mdu	1908	11	α	α	NNP
cord-014597-66vd2mdu	1908	12	,	,	,
cord-014597-66vd2mdu	1908	13	β	β	NN
cord-014597-66vd2mdu	1908	14	,	,	,
cord-014597-66vd2mdu	1908	15	and	and	CC
cord-014597-66vd2mdu	1908	16	γ	γ	NN
cord-014597-66vd2mdu	1908	17	chains	chain	NNS
cord-014597-66vd2mdu	1908	18	were	be	VBD
cord-014597-66vd2mdu	1908	19	expressed	express	VBN
cord-014597-66vd2mdu	1908	20	from	from	IN
cord-014597-66vd2mdu	1908	21	vectors	vector	NNS
cord-014597-66vd2mdu	1908	22	with	with	IN
cord-014597-66vd2mdu	1908	23	marker	marker	NN
cord-014597-66vd2mdu	1908	24	genes	gene	NNS
cord-014597-66vd2mdu	1908	25	for	for	IN
cord-014597-66vd2mdu	1908	26	zeocin	zeocin	NNS
cord-014597-66vd2mdu	1908	27	,	,	,
cord-014597-66vd2mdu	1908	28	blasticidin	blasticidin	NNP
cord-014597-66vd2mdu	1908	29	,	,	,
cord-014597-66vd2mdu	1908	30	and	and	CC
cord-014597-66vd2mdu	1908	31	GS	GS	NNP
cord-014597-66vd2mdu	1908	32	,	,	,
cord-014597-66vd2mdu	1908	33	respectively	respectively	RB
cord-014597-66vd2mdu	1908	34	.	.	.
cord-014597-66vd2mdu	1909	1	A	a	DT
cord-014597-66vd2mdu	1909	2	similar	similar	JJ
cord-014597-66vd2mdu	1909	3	3	3	CD
cord-014597-66vd2mdu	1909	4	vector	vector	NN
cord-014597-66vd2mdu	1909	5	combination	combination	NN
cord-014597-66vd2mdu	1909	6	was	be	VBD
cord-014597-66vd2mdu	1909	7	also	also	RB
cord-014597-66vd2mdu	1909	8	generated	generate	VBN
cord-014597-66vd2mdu	1909	9	with	with	IN
cord-014597-66vd2mdu	1909	10	2GUN	2gun	CD
cord-014597-66vd2mdu	1909	11	elements	element	NNS
cord-014597-66vd2mdu	1909	12	integrated	integrate	VBN
cord-014597-66vd2mdu	1909	13	in	in	IN
cord-014597-66vd2mdu	1909	14	each	each	DT
cord-014597-66vd2mdu	1909	15	vector	vector	NN
cord-014597-66vd2mdu	1909	16	.	.	.
cord-014597-66vd2mdu	1910	1	In	in	IN
cord-014597-66vd2mdu	1910	2	addition	addition	NN
cord-014597-66vd2mdu	1910	3	,	,	,
cord-014597-66vd2mdu	1910	4	2	2	CD
cord-014597-66vd2mdu	1910	5	vectors	vector	NNS
cord-014597-66vd2mdu	1910	6	with	with	IN
cord-014597-66vd2mdu	1910	7	2	2	CD
cord-014597-66vd2mdu	1910	8	subunits	subunit	NNS
cord-014597-66vd2mdu	1910	9	(	(	-LRB-
cord-014597-66vd2mdu	1910	10	γ	γ	NN
cord-014597-66vd2mdu	1910	11	-	-	HYPH
cord-014597-66vd2mdu	1910	12	α	α	NN
cord-014597-66vd2mdu	1910	13	and	and	CC
cord-014597-66vd2mdu	1910	14	α	α	NN
cord-014597-66vd2mdu	1910	15	-	-	HYPH
cord-014597-66vd2mdu	1910	16	γ	γ	NNP
cord-014597-66vd2mdu	1910	17	)	)	-RRB-
cord-014597-66vd2mdu	1910	18	,	,	,
cord-014597-66vd2mdu	1910	19	each	each	DT
cord-014597-66vd2mdu	1910	20	with	with	IN
cord-014597-66vd2mdu	1910	21	a	a	DT
cord-014597-66vd2mdu	1910	22	separate	separate	JJ
cord-014597-66vd2mdu	1910	23	2GUN	2gun	CD
cord-014597-66vd2mdu	1910	24	element	element	NN
cord-014597-66vd2mdu	1910	25	,	,	,
cord-014597-66vd2mdu	1910	26	promoter	promoter	NN
cord-014597-66vd2mdu	1910	27	and	and	CC
cord-014597-66vd2mdu	1910	28	polyadenylation	polyadenylation	NN
cord-014597-66vd2mdu	1910	29	signal	signal	NN
cord-014597-66vd2mdu	1910	30	,	,	,
cord-014597-66vd2mdu	1910	31	were	be	VBD
cord-014597-66vd2mdu	1910	32	generated	generate	VBN
cord-014597-66vd2mdu	1910	33	with	with	IN
cord-014597-66vd2mdu	1910	34	a	a	DT
cord-014597-66vd2mdu	1910	35	GS	GS	NNP
cord-014597-66vd2mdu	1910	36	marker	marker	NN
cord-014597-66vd2mdu	1910	37	gene	gene	NN
cord-014597-66vd2mdu	1910	38	.	.	.
cord-014597-66vd2mdu	1911	1	CHO	CHO	NNP
cord-014597-66vd2mdu	1911	2	GS	GS	NNP
cord-014597-66vd2mdu	1911	3	-/cells	-/cells	NNP
cord-014597-66vd2mdu	1911	4	were	be	VBD
cord-014597-66vd2mdu	1911	5	transfected	transfecte	VBN
cord-014597-66vd2mdu	1911	6	with	with	IN
cord-014597-66vd2mdu	1911	7	the	the	DT
cord-014597-66vd2mdu	1911	8	4	4	CD
cord-014597-66vd2mdu	1911	9	appropriate	appropriate	JJ
cord-014597-66vd2mdu	1911	10	vector	vector	NN
cord-014597-66vd2mdu	1911	11	combinations	combination	NNS
cord-014597-66vd2mdu	1911	12	in	in	IN
cord-014597-66vd2mdu	1911	13	equimolar	equimolar	JJ
cord-014597-66vd2mdu	1911	14	ratios	ratio	NNS
cord-014597-66vd2mdu	1911	15	and	and	CC
cord-014597-66vd2mdu	1911	16	selected	select	VBN
cord-014597-66vd2mdu	1911	17	in	in	IN
cord-014597-66vd2mdu	1911	18	bulk	bulk	NN
cord-014597-66vd2mdu	1911	19	in	in	IN
cord-014597-66vd2mdu	1911	20	medium	medium	JJ
cord-014597-66vd2mdu	1911	21	lacking	lack	VBG
cord-014597-66vd2mdu	1911	22	glutamine	glutamine	NN
cord-014597-66vd2mdu	1911	23	and	and	CC
cord-014597-66vd2mdu	1911	24	1	1	CD
cord-014597-66vd2mdu	1911	25	or	or	CC
cord-014597-66vd2mdu	1911	26	2	2	CD
cord-014597-66vd2mdu	1911	27	antibiotics	antibiotic	NNS
cord-014597-66vd2mdu	1911	28	.	.	.
cord-014597-66vd2mdu	1912	1	The	the	DT
cord-014597-66vd2mdu	1912	2	2-vector	2-vector	CD
cord-014597-66vd2mdu	1912	3	transfected	transfecte	VBD
cord-014597-66vd2mdu	1912	4	cell	cell	NN
cord-014597-66vd2mdu	1912	5	pools	pool	NNS
cord-014597-66vd2mdu	1912	6	recovered	recover	VBD
cord-014597-66vd2mdu	1912	7	first	first	RB
cord-014597-66vd2mdu	1912	8	,	,	,
cord-014597-66vd2mdu	1912	9	due	due	IN
cord-014597-66vd2mdu	1912	10	to	to	IN
cord-014597-66vd2mdu	1912	11	the	the	DT
cord-014597-66vd2mdu	1912	12	presence	presence	NN
cord-014597-66vd2mdu	1912	13	of	of	IN
cord-014597-66vd2mdu	1912	14	only	only	RB
cord-014597-66vd2mdu	1912	15	1	1	CD
cord-014597-66vd2mdu	1912	16	antibiotic	antibiotic	NN
cord-014597-66vd2mdu	1912	17	in	in	IN
cord-014597-66vd2mdu	1912	18	the	the	DT
cord-014597-66vd2mdu	1912	19	medium	medium	NN
cord-014597-66vd2mdu	1912	20	(	(	-LRB-
cord-014597-66vd2mdu	1912	21	Fig	Fig	NNP
cord-014597-66vd2mdu	1912	22	.	.	.
cord-014597-66vd2mdu	1912	23	1a	1a	CD
cord-014597-66vd2mdu	1912	24	)	)	-RRB-
cord-014597-66vd2mdu	1912	25	.	.	.
cord-014597-66vd2mdu	1913	1	The	the	DT
cord-014597-66vd2mdu	1913	2	pools	pool	NNS
cord-014597-66vd2mdu	1913	3	transfected	transfecte	VBN
cord-014597-66vd2mdu	1913	4	with	with	IN
cord-014597-66vd2mdu	1913	5	three	three	CD
cord-014597-66vd2mdu	1913	6	2GUN	2gun	CD
cord-014597-66vd2mdu	1913	7	vectors	vector	NNS
cord-014597-66vd2mdu	1913	8	recovered	recover	VBN
cord-014597-66vd2mdu	1913	9	to	to	TO
cord-014597-66vd2mdu	1913	10	maximum	maximum	VB
cord-014597-66vd2mdu	1913	11	viability	viability	NN
cord-014597-66vd2mdu	1913	12	just	just	RB
cord-014597-66vd2mdu	1913	13	a	a	DT
cord-014597-66vd2mdu	1913	14	few	few	JJ
cord-014597-66vd2mdu	1913	15	days	day	NNS
cord-014597-66vd2mdu	1913	16	after	after	IN
cord-014597-66vd2mdu	1913	17	the	the	DT
cord-014597-66vd2mdu	1913	18	2-vector	2-vector	CD
cord-014597-66vd2mdu	1913	19	2GUN	2gun	CD
cord-014597-66vd2mdu	1913	20	pools	pool	NNS
cord-014597-66vd2mdu	1913	21	.	.	.
cord-014597-66vd2mdu	1914	1	Recovery	recovery	NN
cord-014597-66vd2mdu	1914	2	of	of	IN
cord-014597-66vd2mdu	1914	3	the	the	DT
cord-014597-66vd2mdu	1914	4	Reference	Reference	NNP
cord-014597-66vd2mdu	1914	5	pools	pool	NNS
cord-014597-66vd2mdu	1914	6	took	take	VBD
cord-014597-66vd2mdu	1914	7	up	up	RP
cord-014597-66vd2mdu	1914	8	to	to	IN
cord-014597-66vd2mdu	1914	9	a	a	DT
cord-014597-66vd2mdu	1914	10	week	week	NN
cord-014597-66vd2mdu	1914	11	longer	long	RBR
cord-014597-66vd2mdu	1914	12	than	than	IN
cord-014597-66vd2mdu	1914	13	the	the	DT
cord-014597-66vd2mdu	1914	14	2GUN	2gun	JJ
cord-014597-66vd2mdu	1914	15	pools	pool	NNS
cord-014597-66vd2mdu	1914	16	.	.	.
cord-014597-66vd2mdu	1915	1	Production	production	NN
cord-014597-66vd2mdu	1915	2	of	of	IN
cord-014597-66vd2mdu	1915	3	each	each	DT
cord-014597-66vd2mdu	1915	4	pool	pool	NN
cord-014597-66vd2mdu	1915	5	was	be	VBD
cord-014597-66vd2mdu	1915	6	assessed	assess	VBN
cord-014597-66vd2mdu	1915	7	in	in	IN
cord-014597-66vd2mdu	1915	8	a	a	DT
cord-014597-66vd2mdu	1915	9	batch	batch	NN
cord-014597-66vd2mdu	1915	10	production	production	NN
cord-014597-66vd2mdu	1915	11	run	run	NN
cord-014597-66vd2mdu	1915	12	in	in	IN
cord-014597-66vd2mdu	1915	13	shaker	shaker	NN
cord-014597-66vd2mdu	1915	14	flasks	flask	NNS
cord-014597-66vd2mdu	1915	15	.	.	.
cord-014597-66vd2mdu	1916	1	All	all	DT
cord-014597-66vd2mdu	1916	2	2-vector	2-vector	CD
cord-014597-66vd2mdu	1916	3	2GUN	2gun	CD
cord-014597-66vd2mdu	1916	4	pools	pool	NNS
cord-014597-66vd2mdu	1916	5	which	which	WDT
cord-014597-66vd2mdu	1916	6	recovered	recover	VBD
cord-014597-66vd2mdu	1916	7	first	first	RB
cord-014597-66vd2mdu	1916	8	produced	produce	VBD
cord-014597-66vd2mdu	1916	9	titers	titer	NNS
cord-014597-66vd2mdu	1916	10	around	around	IN
cord-014597-66vd2mdu	1916	11	0.1	0.1	CD
cord-014597-66vd2mdu	1916	12	g	g	NN
cord-014597-66vd2mdu	1916	13	/	/	SYM
cord-014597-66vd2mdu	1916	14	L	L	NNP
cord-014597-66vd2mdu	1916	15	,	,	,
cord-014597-66vd2mdu	1916	16	which	which	WDT
cord-014597-66vd2mdu	1916	17	is	be	VBZ
cord-014597-66vd2mdu	1916	18	almost	almost	RB
cord-014597-66vd2mdu	1916	19	10-fold	10-fold	JJ
cord-014597-66vd2mdu	1916	20	higher	high	JJR
cord-014597-66vd2mdu	1916	21	as	as	IN
cord-014597-66vd2mdu	1916	22	compared	compare	VBN
cord-014597-66vd2mdu	1916	23	to	to	IN
cord-014597-66vd2mdu	1916	24	the	the	DT
cord-014597-66vd2mdu	1916	25	production	production	NN
cord-014597-66vd2mdu	1916	26	by	by	IN
cord-014597-66vd2mdu	1916	27	Reference	reference	NN
cord-014597-66vd2mdu	1916	28	pools	pool	NNS
cord-014597-66vd2mdu	1916	29	(	(	-LRB-
cord-014597-66vd2mdu	1916	30	Fig	fig	NN
cord-014597-66vd2mdu	1916	31	.	.	.
cord-014597-66vd2mdu	1916	32	1b	1b	CD
cord-014597-66vd2mdu	1916	33	)	)	-RRB-
cord-014597-66vd2mdu	1916	34	.	.	.
cord-014597-66vd2mdu	1917	1	The	the	DT
cord-014597-66vd2mdu	1917	2	highest	high	JJS
cord-014597-66vd2mdu	1917	3	titers	titer	NNS
cord-014597-66vd2mdu	1917	4	of	of	IN
cord-014597-66vd2mdu	1917	5	0.5	0.5	CD
cord-014597-66vd2mdu	1917	6	g	g	NN
cord-014597-66vd2mdu	1917	7	/	/	SYM
cord-014597-66vd2mdu	1917	8	L	L	NNP
cord-014597-66vd2mdu	1917	9	were	be	VBD
cord-014597-66vd2mdu	1917	10	obtained	obtain	VBN
cord-014597-66vd2mdu	1917	11	in	in	IN
cord-014597-66vd2mdu	1917	12	the	the	DT
cord-014597-66vd2mdu	1917	13	3-vector	3-vector	CD
cord-014597-66vd2mdu	1917	14	2GUN	2gun	CD
cord-014597-66vd2mdu	1917	15	pools	pool	NNS
cord-014597-66vd2mdu	1917	16	.	.	.
cord-014597-66vd2mdu	1918	1	These	these	DT
cord-014597-66vd2mdu	1918	2	data	datum	NNS
cord-014597-66vd2mdu	1918	3	show	show	VBP
cord-014597-66vd2mdu	1918	4	that	that	IN
cord-014597-66vd2mdu	1918	5	the	the	DT
cord-014597-66vd2mdu	1918	6	2	2	CD
cord-014597-66vd2mdu	1918	7	G	G	NNP
cord-014597-66vd2mdu	1918	8	UNic	UNic	NNP
cord-014597-66vd2mdu	1918	9	™	™	VBD
cord-014597-66vd2mdu	1918	10	genetic	genetic	JJ
cord-014597-66vd2mdu	1918	11	elements	element	NNS
cord-014597-66vd2mdu	1918	12	can	can	MD
cord-014597-66vd2mdu	1918	13	be	be	VB
cord-014597-66vd2mdu	1918	14	successfully	successfully	RB
cord-014597-66vd2mdu	1918	15	used	use	VBN
cord-014597-66vd2mdu	1918	16	to	to	TO
cord-014597-66vd2mdu	1918	17	obtain	obtain	VB
cord-014597-66vd2mdu	1918	18	a	a	DT
cord-014597-66vd2mdu	1918	19	significant	significant	JJ
cord-014597-66vd2mdu	1918	20	increase	increase	NN
cord-014597-66vd2mdu	1918	21	in	in	IN
cord-014597-66vd2mdu	1918	22	the	the	DT
cord-014597-66vd2mdu	1918	23	titer	titer	NN
cord-014597-66vd2mdu	1918	24	of	of	IN
cord-014597-66vd2mdu	1918	25	difficult	difficult	JJ
cord-014597-66vd2mdu	1918	26	-	-	HYPH
cord-014597-66vd2mdu	1918	27	to	to	TO
cord-014597-66vd2mdu	1918	28	-	-	HYPH
cord-014597-66vd2mdu	1918	29	express	express	NN
cord-014597-66vd2mdu	1918	30	proteins	protein	NNS
cord-014597-66vd2mdu	1918	31	.	.	.
cord-014597-66vd2mdu	1919	1	Similar	similar	JJ
cord-014597-66vd2mdu	1919	2	results	result	NNS
cord-014597-66vd2mdu	1919	3	have	have	VBP
cord-014597-66vd2mdu	1919	4	been	be	VBN
cord-014597-66vd2mdu	1919	5	obtained	obtain	VBN
cord-014597-66vd2mdu	1919	6	with	with	IN
cord-014597-66vd2mdu	1919	7	other	other	JJ
cord-014597-66vd2mdu	1919	8	DTE	DTE	NNP
cord-014597-66vd2mdu	1919	9	proteins	protein	NNS
cord-014597-66vd2mdu	1919	10	,	,	,
cord-014597-66vd2mdu	1919	11	including	include	VBG
cord-014597-66vd2mdu	1919	12	fc	fc	NN
cord-014597-66vd2mdu	1919	13	-	-	HYPH
cord-014597-66vd2mdu	1919	14	fusion	fusion	NN
cord-014597-66vd2mdu	1919	15	proteins	protein	NNS
cord-014597-66vd2mdu	1919	16	and	and	CC
cord-014597-66vd2mdu	1919	17	bi	bi	JJ
cord-014597-66vd2mdu	1919	18	-	-	JJ
cord-014597-66vd2mdu	1919	19	specific	specific	JJ
cord-014597-66vd2mdu	1919	20	antibodies	antibody	NNS
cord-014597-66vd2mdu	1919	21	(	(	-LRB-
cord-014597-66vd2mdu	1919	22	not	not	RB
cord-014597-66vd2mdu	1919	23	shown	show	VBN
cord-014597-66vd2mdu	1919	24	)	)	-RRB-
cord-014597-66vd2mdu	1919	25	.	.	.
cord-014597-66vd2mdu	1920	1	The	the	DT
cord-014597-66vd2mdu	1920	2	expression	expression	NN
cord-014597-66vd2mdu	1920	3	of	of	IN
cord-014597-66vd2mdu	1920	4	a	a	DT
cord-014597-66vd2mdu	1920	5	large	large	JJ
cord-014597-66vd2mdu	1920	6	,	,	,
cord-014597-66vd2mdu	1920	7	glycosylated	glycosylated	JJ
cord-014597-66vd2mdu	1920	8	multimeric	multimeric	NN
cord-014597-66vd2mdu	1920	9	difficult	difficult	JJ
cord-014597-66vd2mdu	1920	10	to	to	TO
cord-014597-66vd2mdu	1920	11	express	express	VB
cord-014597-66vd2mdu	1920	12	protein	protein	NN
cord-014597-66vd2mdu	1920	13	can	can	MD
cord-014597-66vd2mdu	1920	14	be	be	VB
cord-014597-66vd2mdu	1920	15	increased	increase	VBN
cord-014597-66vd2mdu	1920	16	more	more	JJR
cord-014597-66vd2mdu	1920	17	than	than	IN
cord-014597-66vd2mdu	1920	18	ten	ten	CD
cord-014597-66vd2mdu	1920	19	-	-	HYPH
cord-014597-66vd2mdu	1920	20	fold	fold	NN
cord-014597-66vd2mdu	1920	21	in	in	IN
cord-014597-66vd2mdu	1920	22	CHO	CHO	NNP
cord-014597-66vd2mdu	1920	23	GS	GS	NNP
cord-014597-66vd2mdu	1920	24	pools	pool	NNS
cord-014597-66vd2mdu	1920	25	by	by	IN
cord-014597-66vd2mdu	1920	26	application	application	NN
cord-014597-66vd2mdu	1920	27	of	of	IN
cord-014597-66vd2mdu	1920	28	2	2	CD
cord-014597-66vd2mdu	1920	29	G	G	NNP
cord-014597-66vd2mdu	1920	30	UNic	UNic	NNP
cord-014597-66vd2mdu	1920	31	™	™	VBD
cord-014597-66vd2mdu	1920	32	genetic	genetic	JJ
cord-014597-66vd2mdu	1920	33	elements	element	NNS
cord-014597-66vd2mdu	1920	34	.	.	.
cord-014597-66vd2mdu	1921	1	The	the	DT
cord-014597-66vd2mdu	1921	2	highest	high	JJS
cord-014597-66vd2mdu	1921	3	expression	expression	NN
cord-014597-66vd2mdu	1921	4	of	of	IN
cord-014597-66vd2mdu	1921	5	is	be	VBZ
cord-014597-66vd2mdu	1921	6	obtained	obtain	VBN
cord-014597-66vd2mdu	1921	7	using	use	VBG
cord-014597-66vd2mdu	1921	8	a	a	DT
cord-014597-66vd2mdu	1921	9	separate	separate	JJ
cord-014597-66vd2mdu	1921	10	vector	vector	NN
cord-014597-66vd2mdu	1921	11	for	for	IN
cord-014597-66vd2mdu	1921	12	each	each	DT
cord-014597-66vd2mdu	1921	13	subunit	subunit	NN
cord-014597-66vd2mdu	1921	14	.	.	.
cord-014597-66vd2mdu	1922	1	Characterization	characterization	NN
cord-014597-66vd2mdu	1922	2	of	of	IN
cord-014597-66vd2mdu	1922	3	antibody	antibody	NN
cord-014597-66vd2mdu	1922	4	-	-	HYPH
cord-014597-66vd2mdu	1922	5	producing	produce	VBG
cord-014597-66vd2mdu	1922	6	CHO	CHO	NNP
cord-014597-66vd2mdu	1922	7	cells	cell	NNS
cord-014597-66vd2mdu	1922	8	with	with	IN
cord-014597-66vd2mdu	1922	9	chromosome	chromosome	NN
cord-014597-66vd2mdu	1922	10	aneuploidy	aneuploidy	NN
cord-014597-66vd2mdu	1923	1	Noriko	Noriko	NNP
cord-014597-66vd2mdu	1923	2	Yamano	Yamano	NNP
cord-014597-66vd2mdu	1923	3	1,2	1,2	CD
cord-014597-66vd2mdu	1923	4	,	,	,
cord-014597-66vd2mdu	1923	5	Sho	Sho	NNP
cord-014597-66vd2mdu	1923	6	Tanaka	Tanaka	NNP
cord-014597-66vd2mdu	1923	7	Background	Background	NNP
cord-014597-66vd2mdu	1923	8	Chinese	chinese	JJ
cord-014597-66vd2mdu	1923	9	hamster	hamster	NN
cord-014597-66vd2mdu	1923	10	ovary	ovary	NN
cord-014597-66vd2mdu	1923	11	(	(	-LRB-
cord-014597-66vd2mdu	1923	12	CHO	CHO	NNP
cord-014597-66vd2mdu	1923	13	)	)	-RRB-
cord-014597-66vd2mdu	1923	14	cells	cell	NNS
cord-014597-66vd2mdu	1923	15	are	be	VBP
cord-014597-66vd2mdu	1923	16	commonly	commonly	RB
cord-014597-66vd2mdu	1923	17	used	use	VBN
cord-014597-66vd2mdu	1923	18	as	as	IN
cord-014597-66vd2mdu	1923	19	host	host	NN
cord-014597-66vd2mdu	1923	20	cells	cell	NNS
cord-014597-66vd2mdu	1923	21	to	to	TO
cord-014597-66vd2mdu	1923	22	produce	produce	VB
cord-014597-66vd2mdu	1923	23	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	1923	24	.	.	.
cord-014597-66vd2mdu	1924	1	However	however	RB
cord-014597-66vd2mdu	1924	2	,	,	,
cord-014597-66vd2mdu	1924	3	the	the	DT
cord-014597-66vd2mdu	1924	4	number	number	NN
cord-014597-66vd2mdu	1924	5	of	of	IN
cord-014597-66vd2mdu	1924	6	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	1924	7	in	in	IN
cord-014597-66vd2mdu	1924	8	CHO	CHO	NNP
cord-014597-66vd2mdu	1924	9	cells	cell	NNS
cord-014597-66vd2mdu	1924	10	varies	vary	VBZ
cord-014597-66vd2mdu	1924	11	.	.	.
cord-014597-66vd2mdu	1925	1	Previously	previously	RB
cord-014597-66vd2mdu	1925	2	,	,	,
cord-014597-66vd2mdu	1925	3	DG44-SC20	DG44-SC20	NNP
cord-014597-66vd2mdu	1925	4	and	and	CC
cord-014597-66vd2mdu	1925	5	DG44-SC39	dg44-sc39	JJ
cord-014597-66vd2mdu	1925	6	cell	cell	NN
cord-014597-66vd2mdu	1925	7	lines	line	NNS
cord-014597-66vd2mdu	1925	8	with	with	IN
cord-014597-66vd2mdu	1925	9	modal	modal	JJ
cord-014597-66vd2mdu	1925	10	chromosome	chromosome	NN
cord-014597-66vd2mdu	1925	11	numbers	number	NNS
cord-014597-66vd2mdu	1925	12	of	of	IN
cord-014597-66vd2mdu	1925	13	20	20	CD
cord-014597-66vd2mdu	1925	14	and	and	CC
cord-014597-66vd2mdu	1925	15	39	39	CD
cord-014597-66vd2mdu	1925	16	were	be	VBD
cord-014597-66vd2mdu	1925	17	isolated	isolate	VBN
cord-014597-66vd2mdu	1925	18	from	from	IN
cord-014597-66vd2mdu	1925	19	parental	parental	JJ
cord-014597-66vd2mdu	1925	20	CHO	CHO	NNP
cord-014597-66vd2mdu	1925	21	-	-	HYPH
cord-014597-66vd2mdu	1925	22	DG44	DG44	NNP
cord-014597-66vd2mdu	1925	23	cells	cell	NNS
cord-014597-66vd2mdu	1925	24	,	,	,
cord-014597-66vd2mdu	1925	25	from	from	IN
cord-014597-66vd2mdu	1925	26	which	which	WDT
cord-014597-66vd2mdu	1925	27	IgG3-expressing	igg3-expresse	VBG
cord-014597-66vd2mdu	1925	28	cell	cell	NN
cord-014597-66vd2mdu	1925	29	lines	line	NNS
cord-014597-66vd2mdu	1925	30	named	name	VBN
cord-014597-66vd2mdu	1925	31	IgG3-SC20	IgG3-SC20	NNP
cord-014597-66vd2mdu	1925	32	and	and	CC
cord-014597-66vd2mdu	1925	33	IgG3-SC39	IgG3-SC39	NNP
cord-014597-66vd2mdu	1925	34	were	be	VBD
cord-014597-66vd2mdu	1925	35	established	establish	VBN
cord-014597-66vd2mdu	1925	36	,	,	,
cord-014597-66vd2mdu	1925	37	respectively	respectively	RB
cord-014597-66vd2mdu	1925	38	.	.	.
cord-014597-66vd2mdu	1926	1	The	the	DT
cord-014597-66vd2mdu	1926	2	IgG3-SC39	IgG3-SC39	NNP
cord-014597-66vd2mdu	1926	3	cell	cell	NN
cord-014597-66vd2mdu	1926	4	pool	pool	NN
cord-014597-66vd2mdu	1926	5	showed	show	VBD
cord-014597-66vd2mdu	1926	6	a	a	DT
cord-014597-66vd2mdu	1926	7	higher	higher	RBR
cord-014597-66vd2mdu	1926	8	specific	specific	JJ
cord-014597-66vd2mdu	1926	9	IgG3	igg3	NN
cord-014597-66vd2mdu	1926	10	production	production	NN
cord-014597-66vd2mdu	1926	11	rate	rate	NN
cord-014597-66vd2mdu	1926	12	than	than	IN
cord-014597-66vd2mdu	1926	13	the	the	DT
cord-014597-66vd2mdu	1926	14	IgG3-SC20	IgG3-SC20	``
cord-014597-66vd2mdu	1926	15	cell	cell	NN
cord-014597-66vd2mdu	1926	16	pool	pool	NN
cord-014597-66vd2mdu	1926	17	[	[	-LRB-
cord-014597-66vd2mdu	1926	18	1	1	CD
cord-014597-66vd2mdu	1926	19	]	]	-RRB-
cord-014597-66vd2mdu	1926	20	.	.	.
cord-014597-66vd2mdu	1927	1	Even	even	RB
cord-014597-66vd2mdu	1927	2	though	though	IN
cord-014597-66vd2mdu	1927	3	all	all	DT
cord-014597-66vd2mdu	1927	4	of	of	IN
cord-014597-66vd2mdu	1927	5	the	the	DT
cord-014597-66vd2mdu	1927	6	IgG3-SC20	IgG3-SC20	NNP
cord-014597-66vd2mdu	1927	7	clones	clone	NNS
cord-014597-66vd2mdu	1927	8	and	and	CC
cord-014597-66vd2mdu	1927	9	half	half	NN
cord-014597-66vd2mdu	1927	10	of	of	IN
cord-014597-66vd2mdu	1927	11	the	the	DT
cord-014597-66vd2mdu	1927	12	IgG3-SC39	IgG3-SC39	NNP
cord-014597-66vd2mdu	1927	13	clones	clone	NNS
cord-014597-66vd2mdu	1927	14	contained	contain	VBD
cord-014597-66vd2mdu	1927	15	the	the	DT
cord-014597-66vd2mdu	1927	16	same	same	JJ
cord-014597-66vd2mdu	1927	17	number	number	NN
cord-014597-66vd2mdu	1927	18	of	of	IN
cord-014597-66vd2mdu	1927	19	vector	vector	NN
cord-014597-66vd2mdu	1927	20	integration	integration	NN
cord-014597-66vd2mdu	1927	21	sites	site	NNS
cord-014597-66vd2mdu	1927	22	(	(	-LRB-
cord-014597-66vd2mdu	1927	23	single	single	JJ
cord-014597-66vd2mdu	1927	24	integration	integration	NN
cord-014597-66vd2mdu	1927	25	site	site	NN
cord-014597-66vd2mdu	1927	26	)	)	-RRB-
cord-014597-66vd2mdu	1927	27	,	,	,
cord-014597-66vd2mdu	1927	28	IgG	igg	NN
cord-014597-66vd2mdu	1927	29	-	-	HYPH
cord-014597-66vd2mdu	1927	30	SC39	SC39	NNP
cord-014597-66vd2mdu	1927	31	cell	cell	NN
cord-014597-66vd2mdu	1927	32	clones	clone	NNS
cord-014597-66vd2mdu	1927	33	produced	produce	VBD
cord-014597-66vd2mdu	1927	34	more	more	JJR
cord-014597-66vd2mdu	1927	35	IgG3	igg3	NN
cord-014597-66vd2mdu	1927	36	following	follow	VBG
cord-014597-66vd2mdu	1927	37	the	the	DT
cord-014597-66vd2mdu	1927	38	culture	culture	NN
cord-014597-66vd2mdu	1927	39	of	of	IN
cord-014597-66vd2mdu	1927	40	single	single	JJ
cord-014597-66vd2mdu	1927	41	-	-	HYPH
cord-014597-66vd2mdu	1927	42	cell	cell	NN
cord-014597-66vd2mdu	1927	43	clones	clone	NNS
cord-014597-66vd2mdu	1927	44	than	than	IN
cord-014597-66vd2mdu	1927	45	any	any	DT
cord-014597-66vd2mdu	1927	46	of	of	IN
cord-014597-66vd2mdu	1927	47	the	the	DT
cord-014597-66vd2mdu	1927	48	IgG3-SC20	IgG3-SC20	NNP
cord-014597-66vd2mdu	1927	49	clones	clone	NNS
cord-014597-66vd2mdu	1927	50	[	[	-LRB-
cord-014597-66vd2mdu	1927	51	1	1	CD
cord-014597-66vd2mdu	1927	52	]	]	-RRB-
cord-014597-66vd2mdu	1927	53	.	.	.
cord-014597-66vd2mdu	1928	1	In	in	IN
cord-014597-66vd2mdu	1928	2	this	this	DT
cord-014597-66vd2mdu	1928	3	study	study	NN
cord-014597-66vd2mdu	1928	4	,	,	,
cord-014597-66vd2mdu	1928	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1928	6	performed	perform	VBD
cord-014597-66vd2mdu	1928	7	transcriptome	transcriptome	NN
cord-014597-66vd2mdu	1928	8	analysis	analysis	NN
cord-014597-66vd2mdu	1928	9	to	to	TO
cord-014597-66vd2mdu	1928	10	investigate	investigate	VB
cord-014597-66vd2mdu	1928	11	the	the	DT
cord-014597-66vd2mdu	1928	12	characteristics	characteristic	NNS
cord-014597-66vd2mdu	1928	13	of	of	IN
cord-014597-66vd2mdu	1928	14	high	high	JJ
cord-014597-66vd2mdu	1928	15	-	-	HYPH
cord-014597-66vd2mdu	1928	16	producer	producer	NN
cord-014597-66vd2mdu	1928	17	cells	cell	NNS
cord-014597-66vd2mdu	1928	18	with	with	IN
cord-014597-66vd2mdu	1928	19	chromosome	chromosome	NN
cord-014597-66vd2mdu	1928	20	aneuploidy	aneuploidy	NN
cord-014597-66vd2mdu	1928	21	.	.	.
cord-014597-66vd2mdu	1929	1	Transcriptome	Transcriptome	NNP
cord-014597-66vd2mdu	1929	2	analyses	analysis	NNS
cord-014597-66vd2mdu	1929	3	using	use	VBG
cord-014597-66vd2mdu	1929	4	amplified	amplify	VBN
cord-014597-66vd2mdu	1929	5	fragment	fragment	NN
cord-014597-66vd2mdu	1929	6	length	length	NN
cord-014597-66vd2mdu	1929	7	polymorphism	polymorphism	NN
cord-014597-66vd2mdu	1929	8	(	(	-LRB-
cord-014597-66vd2mdu	1929	9	AFLP)-based	aflp)-based	JJ
cord-014597-66vd2mdu	1929	10	high	high	JJ
cord-014597-66vd2mdu	1929	11	-	-	HYPH
cord-014597-66vd2mdu	1929	12	coverage	coverage	NN
cord-014597-66vd2mdu	1929	13	expression	expression	NN
cord-014597-66vd2mdu	1929	14	profiling	profiling	NN
cord-014597-66vd2mdu	1929	15	(	(	-LRB-
cord-014597-66vd2mdu	1929	16	HiCEP	HiCEP	NNP
cord-014597-66vd2mdu	1929	17	)	)	-RRB-
cord-014597-66vd2mdu	1929	18	and	and	CC
cord-014597-66vd2mdu	1930	1	de	de	NNP
cord-014597-66vd2mdu	1930	2	novo	novo	NNP
cord-014597-66vd2mdu	1930	3	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1930	4	-	-	HYPH
cord-014597-66vd2mdu	1930	5	seq	seq	NNP
cord-014597-66vd2mdu	1930	6	were	be	VBD
cord-014597-66vd2mdu	1930	7	performed	perform	VBN
cord-014597-66vd2mdu	1930	8	on	on	IN
cord-014597-66vd2mdu	1930	9	DG44-SC20	DG44-SC20	NNP
cord-014597-66vd2mdu	1930	10	,	,	,
cord-014597-66vd2mdu	1930	11	DG44-SC39	DG44-SC39	NNP
cord-014597-66vd2mdu	1930	12	,	,	,
cord-014597-66vd2mdu	1930	13	IgG3-SC20	IgG3-SC20	''
cord-014597-66vd2mdu	1930	14	and	and	CC
cord-014597-66vd2mdu	1930	15	IgG3-SC39	IgG3-SC39	NNP
cord-014597-66vd2mdu	1930	16	.	.	.
cord-014597-66vd2mdu	1931	1	To	to	TO
cord-014597-66vd2mdu	1931	2	compare	compare	VB
cord-014597-66vd2mdu	1931	3	cell	cell	NN
cord-014597-66vd2mdu	1931	4	lines	line	NNS
cord-014597-66vd2mdu	1931	5	with	with	IN
cord-014597-66vd2mdu	1931	6	different	different	JJ
cord-014597-66vd2mdu	1931	7	numbers	number	NNS
cord-014597-66vd2mdu	1931	8	of	of	IN
cord-014597-66vd2mdu	1931	9	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	1931	10	,	,	,
cord-014597-66vd2mdu	1931	11	transcriptome	transcriptome	NN
cord-014597-66vd2mdu	1931	12	data	datum	NNS
cord-014597-66vd2mdu	1931	13	from	from	IN
cord-014597-66vd2mdu	1931	14	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1931	15	-	-	HYPH
cord-014597-66vd2mdu	1931	16	seq	seq	NNP
cord-014597-66vd2mdu	1931	17	were	be	VBD
cord-014597-66vd2mdu	1931	18	adjusted	adjust	VBN
cord-014597-66vd2mdu	1931	19	for	for	IN
cord-014597-66vd2mdu	1931	20	cell	cell	NN
cord-014597-66vd2mdu	1931	21	number	number	NN
cord-014597-66vd2mdu	1931	22	using	use	VBG
cord-014597-66vd2mdu	1931	23	RNA	RNA	NNP
cord-014597-66vd2mdu	1931	24	reference	reference	NN
cord-014597-66vd2mdu	1931	25	materials	material	NNS
cord-014597-66vd2mdu	1931	26	(	(	-LRB-
cord-014597-66vd2mdu	1931	27	NMIJ	NMIJ	NNP
cord-014597-66vd2mdu	1931	28	CRM	CRM	NNP
cord-014597-66vd2mdu	1931	29	6204-a	6204-a	CD
cord-014597-66vd2mdu	1931	30	;	;	:
cord-014597-66vd2mdu	1931	31	National	National	NNP
cord-014597-66vd2mdu	1931	32	Institute	Institute	NNP
cord-014597-66vd2mdu	1931	33	of	of	IN
cord-014597-66vd2mdu	1931	34	Advanced	Advanced	NNP
cord-014597-66vd2mdu	1931	35	Industrial	Industrial	NNP
cord-014597-66vd2mdu	1931	36	Science	Science	NNP
cord-014597-66vd2mdu	1931	37	and	and	CC
cord-014597-66vd2mdu	1931	38	Technology	Technology	NNP
cord-014597-66vd2mdu	1931	39	)	)	-RRB-
cord-014597-66vd2mdu	1931	40	mixed	mix	VBN
cord-014597-66vd2mdu	1931	41	at	at	IN
cord-014597-66vd2mdu	1931	42	equal	equal	JJ
cord-014597-66vd2mdu	1931	43	amounts	amount	NNS
cord-014597-66vd2mdu	1931	44	per	per	IN
cord-014597-66vd2mdu	1931	45	cell	cell	NN
cord-014597-66vd2mdu	1931	46	.	.	.
cord-014597-66vd2mdu	1932	1	Pathways	pathway	NNS
cord-014597-66vd2mdu	1932	2	related	relate	VBN
cord-014597-66vd2mdu	1932	3	to	to	IN
cord-014597-66vd2mdu	1932	4	differentially	differentially	RB
cord-014597-66vd2mdu	1932	5	expressed	express	VBN
cord-014597-66vd2mdu	1932	6	genes	gene	NNS
cord-014597-66vd2mdu	1932	7	were	be	VBD
cord-014597-66vd2mdu	1932	8	searched	search	VBN
cord-014597-66vd2mdu	1932	9	using	use	VBG
cord-014597-66vd2mdu	1932	10	KeyMolnet	KeyMolnet	NNP
cord-014597-66vd2mdu	1932	11	(	(	-LRB-
cord-014597-66vd2mdu	1932	12	KM	KM	NNP
cord-014597-66vd2mdu	1932	13	Data	Data	NNP
cord-014597-66vd2mdu	1932	14	)	)	-RRB-
cord-014597-66vd2mdu	1932	15	.	.	.
cord-014597-66vd2mdu	1933	1	High	high	JJ
cord-014597-66vd2mdu	1933	2	-	-	HYPH
cord-014597-66vd2mdu	1933	3	chromosome	chromosome	NN
cord-014597-66vd2mdu	1933	4	-	-	HYPH
cord-014597-66vd2mdu	1933	5	number	number	NN
cord-014597-66vd2mdu	1933	6	CHO	CHO	NNP
cord-014597-66vd2mdu	1933	7	cells	cell	NNS
cord-014597-66vd2mdu	1933	8	showed	show	VBD
cord-014597-66vd2mdu	1933	9	larger	large	JJR
cord-014597-66vd2mdu	1933	10	cell	cell	NN
cord-014597-66vd2mdu	1933	11	diameters	diameter	NNS
cord-014597-66vd2mdu	1933	12	,	,	,
cord-014597-66vd2mdu	1933	13	as	as	IN
cord-014597-66vd2mdu	1933	14	determined	determine	VBN
cord-014597-66vd2mdu	1933	15	by	by	IN
cord-014597-66vd2mdu	1933	16	Vi	Vi	NNP
cord-014597-66vd2mdu	1933	17	-	-	HYPH
cord-014597-66vd2mdu	1933	18	cell	cell	NN
cord-014597-66vd2mdu	1933	19	(	(	-LRB-
cord-014597-66vd2mdu	1933	20	Beckman	Beckman	NNP
cord-014597-66vd2mdu	1933	21	Coulter	Coulter	NNP
cord-014597-66vd2mdu	1933	22	)	)	-RRB-
cord-014597-66vd2mdu	1933	23	measurement	measurement	NN
cord-014597-66vd2mdu	1933	24	.	.	.
cord-014597-66vd2mdu	1934	1	The	the	DT
cord-014597-66vd2mdu	1934	2	predicted	predict	VBN
cord-014597-66vd2mdu	1934	3	volume	volume	NN
cord-014597-66vd2mdu	1934	4	ratios	ratio	NNS
cord-014597-66vd2mdu	1934	5	,	,	,
cord-014597-66vd2mdu	1934	6	based	base	VBN
cord-014597-66vd2mdu	1934	7	on	on	IN
cord-014597-66vd2mdu	1934	8	these	these	DT
cord-014597-66vd2mdu	1934	9	diameters	diameter	NNS
cord-014597-66vd2mdu	1934	10	,	,	,
cord-014597-66vd2mdu	1934	11	are	be	VBP
cord-014597-66vd2mdu	1934	12	2.24	2.24	CD
cord-014597-66vd2mdu	1935	1	(	(	-LRB-
cord-014597-66vd2mdu	1935	2	DG44-SC39	DG44-SC39	NNP
cord-014597-66vd2mdu	1935	3	:	:	:
cord-014597-66vd2mdu	1935	4	DG44-SC20	DG44-SC20	NNP
cord-014597-66vd2mdu	1935	5	)	)	-RRB-
cord-014597-66vd2mdu	1935	6	and	and	CC
cord-014597-66vd2mdu	1935	7	1.59	1.59	CD
cord-014597-66vd2mdu	1935	8	(	(	-LRB-
cord-014597-66vd2mdu	1935	9	IgG3-SC39	igg3-sc39	CD
cord-014597-66vd2mdu	1935	10	:	:	:
cord-014597-66vd2mdu	1935	11	IgG3-SC20	IgG3-SC20	''
cord-014597-66vd2mdu	1935	12	)	)	-RRB-
cord-014597-66vd2mdu	1935	13	.	.	.
cord-014597-66vd2mdu	1936	1	The	the	DT
cord-014597-66vd2mdu	1936	2	levels	level	NNS
cord-014597-66vd2mdu	1936	3	of	of	IN
cord-014597-66vd2mdu	1936	4	β	β	NN
cord-014597-66vd2mdu	1936	5	-	-	HYPH
cord-014597-66vd2mdu	1936	6	actin	actin	NN
cord-014597-66vd2mdu	1936	7	and	and	CC
cord-014597-66vd2mdu	1936	8	the	the	DT
cord-014597-66vd2mdu	1936	9	products	product	NNS
cord-014597-66vd2mdu	1936	10	of	of	IN
cord-014597-66vd2mdu	1936	11	most	most	JJS
cord-014597-66vd2mdu	1936	12	other	other	JJ
cord-014597-66vd2mdu	1936	13	genes	gene	NNS
cord-014597-66vd2mdu	1936	14	that	that	WDT
cord-014597-66vd2mdu	1936	15	were	be	VBD
cord-014597-66vd2mdu	1936	16	detected	detect	VBN
cord-014597-66vd2mdu	1936	17	by	by	IN
cord-014597-66vd2mdu	1936	18	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1936	19	-	-	HYPH
cord-014597-66vd2mdu	1936	20	seq	seq	NNP
cord-014597-66vd2mdu	1936	21	differed	differ	VBD
cord-014597-66vd2mdu	1936	22	by	by	IN
cord-014597-66vd2mdu	1936	23	approximately	approximately	RB
cord-014597-66vd2mdu	1936	24	20	20	CD
cord-014597-66vd2mdu	1936	25	%	%	NN
cord-014597-66vd2mdu	1936	26	in	in	IN
cord-014597-66vd2mdu	1936	27	the	the	DT
cord-014597-66vd2mdu	1936	28	comparison	comparison	NN
cord-014597-66vd2mdu	1936	29	between	between	IN
cord-014597-66vd2mdu	1936	30	SC39	SC39	NNP
cord-014597-66vd2mdu	1936	31	and	and	CC
cord-014597-66vd2mdu	1936	32	SC20	SC20	NNP
cord-014597-66vd2mdu	1937	1	(	(	-LRB-
cord-014597-66vd2mdu	1937	2	SC39	SC39	NNP
cord-014597-66vd2mdu	1937	3	>	>	XX
cord-014597-66vd2mdu	1937	4	SC20	SC20	NNP
cord-014597-66vd2mdu	1937	5	)	)	-RRB-
cord-014597-66vd2mdu	1937	6	.	.	.
cord-014597-66vd2mdu	1938	1	Based	base	VBN
cord-014597-66vd2mdu	1938	2	on	on	IN
cord-014597-66vd2mdu	1938	3	the	the	DT
cord-014597-66vd2mdu	1938	4	analysis	analysis	NN
cord-014597-66vd2mdu	1938	5	of	of	IN
cord-014597-66vd2mdu	1938	6	gene	gene	NN
cord-014597-66vd2mdu	1938	7	expression	expression	NN
cord-014597-66vd2mdu	1938	8	levels	level	NNS
cord-014597-66vd2mdu	1938	9	per	per	IN
cord-014597-66vd2mdu	1938	10	cell	cell	NN
cord-014597-66vd2mdu	1938	11	volume	volume	NN
cord-014597-66vd2mdu	1938	12	,	,	,
cord-014597-66vd2mdu	1938	13	approximately	approximately	RB
cord-014597-66vd2mdu	1938	14	90	90	CD
cord-014597-66vd2mdu	1938	15	%	%	NN
cord-014597-66vd2mdu	1938	16	of	of	IN
cord-014597-66vd2mdu	1938	17	detected	detect	VBN
cord-014597-66vd2mdu	1938	18	genes	gene	NNS
cord-014597-66vd2mdu	1938	19	showed	show	VBD
cord-014597-66vd2mdu	1938	20	lower	low	JJR
cord-014597-66vd2mdu	1938	21	expression	expression	NN
cord-014597-66vd2mdu	1938	22	in	in	IN
cord-014597-66vd2mdu	1938	23	both	both	DT
cord-014597-66vd2mdu	1938	24	DG44-SC39	dg44-sc39	NN
cord-014597-66vd2mdu	1938	25	and	and	CC
cord-014597-66vd2mdu	1938	26	IgG3-SC39	igg3-sc39	CD
cord-014597-66vd2mdu	1938	27	compared	compare	VBN
cord-014597-66vd2mdu	1938	28	with	with	IN
cord-014597-66vd2mdu	1938	29	the	the	DT
cord-014597-66vd2mdu	1938	30	levels	level	NNS
cord-014597-66vd2mdu	1938	31	in	in	IN
cord-014597-66vd2mdu	1938	32	DG44-SC20	DG44-SC20	NNP
cord-014597-66vd2mdu	1938	33	and	and	CC
cord-014597-66vd2mdu	1938	34	IgG3-SC20	IgG3-SC20	NNP
cord-014597-66vd2mdu	1938	35	,	,	,
cord-014597-66vd2mdu	1938	36	respectively	respectively	RB
cord-014597-66vd2mdu	1938	37	.	.	.
cord-014597-66vd2mdu	1939	1	In	in	IN
cord-014597-66vd2mdu	1939	2	addition	addition	NN
cord-014597-66vd2mdu	1939	3	,	,	,
cord-014597-66vd2mdu	1939	4	the	the	DT
cord-014597-66vd2mdu	1939	5	number	number	NN
cord-014597-66vd2mdu	1939	6	of	of	IN
cord-014597-66vd2mdu	1939	7	genes	gene	NNS
cord-014597-66vd2mdu	1939	8	whose	whose	WP$
cord-014597-66vd2mdu	1939	9	expression	expression	NN
cord-014597-66vd2mdu	1939	10	level	level	NN
cord-014597-66vd2mdu	1939	11	was	be	VBD
cord-014597-66vd2mdu	1939	12	decreased	decrease	VBN
cord-014597-66vd2mdu	1939	13	in	in	IN
cord-014597-66vd2mdu	1939	14	IgG3-SC39	igg3-sc39	CD
cord-014597-66vd2mdu	1939	15	compared	compare	VBN
cord-014597-66vd2mdu	1939	16	with	with	IN
cord-014597-66vd2mdu	1939	17	that	that	DT
cord-014597-66vd2mdu	1939	18	in	in	IN
cord-014597-66vd2mdu	1939	19	DG44-SC39	dg44-sc39	NN
cord-014597-66vd2mdu	1939	20	was	be	VBD
cord-014597-66vd2mdu	1939	21	larger	large	JJR
cord-014597-66vd2mdu	1939	22	than	than	IN
cord-014597-66vd2mdu	1939	23	those	those	DT
cord-014597-66vd2mdu	1939	24	showing	show	VBG
cord-014597-66vd2mdu	1939	25	the	the	DT
cord-014597-66vd2mdu	1939	26	opposite	opposite	JJ
cord-014597-66vd2mdu	1939	27	pattern	pattern	NN
cord-014597-66vd2mdu	1939	28	.	.	.
cord-014597-66vd2mdu	1940	1	The	the	DT
cord-014597-66vd2mdu	1940	2	results	result	NNS
cord-014597-66vd2mdu	1940	3	of	of	IN
cord-014597-66vd2mdu	1940	4	the	the	DT
cord-014597-66vd2mdu	1940	5	comparisons	comparison	NNS
cord-014597-66vd2mdu	1940	6	between	between	IN
cord-014597-66vd2mdu	1940	7	IgG3-SC20	igg3-sc20	$
cord-014597-66vd2mdu	1940	8	and	and	CC
cord-014597-66vd2mdu	1940	9	IgG3-SC39	IgG3-SC39	NNP
cord-014597-66vd2mdu	1940	10	indicate	indicate	VBP
cord-014597-66vd2mdu	1940	11	that	that	IN
cord-014597-66vd2mdu	1940	12	differentially	differentially	RB
cord-014597-66vd2mdu	1940	13	expressed	express	VBN
cord-014597-66vd2mdu	1940	14	genes	gene	NNS
cord-014597-66vd2mdu	1940	15	were	be	VBD
cord-014597-66vd2mdu	1940	16	mainly	mainly	RB
cord-014597-66vd2mdu	1940	17	related	relate	VBN
cord-014597-66vd2mdu	1940	18	to	to	IN
cord-014597-66vd2mdu	1940	19	cell	cell	NN
cord-014597-66vd2mdu	1940	20	growth	growth	NN
cord-014597-66vd2mdu	1940	21	(	(	-LRB-
cord-014597-66vd2mdu	1940	22	e.g.	e.g.	RB
cord-014597-66vd2mdu	1940	23	Myc	Myc	NNP
cord-014597-66vd2mdu	1940	24	,	,	,
cord-014597-66vd2mdu	1940	25	SMAD	SMAD	NNP
cord-014597-66vd2mdu	1940	26	)	)	-RRB-
cord-014597-66vd2mdu	1940	27	,	,	,
cord-014597-66vd2mdu	1940	28	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	1940	29	(	(	-LRB-
cord-014597-66vd2mdu	1940	30	e.g.	e.g.	DT
cord-014597-66vd2mdu	1940	31	Caspase	Caspase	NNP
cord-014597-66vd2mdu	1940	32	)	)	-RRB-
cord-014597-66vd2mdu	1940	33	,	,	,
cord-014597-66vd2mdu	1940	34	lipid	lipid	NN
cord-014597-66vd2mdu	1940	35	metabolism	metabolism	NN
cord-014597-66vd2mdu	1940	36	(	(	-LRB-
cord-014597-66vd2mdu	1940	37	e.g.	e.g.	RB
cord-014597-66vd2mdu	1940	38	SREBP	SREBP	NNP
cord-014597-66vd2mdu	1940	39	,	,	,
cord-014597-66vd2mdu	1940	40	PPARγ	PPARγ	NNS
cord-014597-66vd2mdu	1940	41	)	)	-RRB-
cord-014597-66vd2mdu	1940	42	and	and	CC
cord-014597-66vd2mdu	1940	43	epigenetic	epigenetic	JJ
cord-014597-66vd2mdu	1940	44	histone	histone	NN
cord-014597-66vd2mdu	1940	45	modification	modification	NN
cord-014597-66vd2mdu	1940	46	(	(	-LRB-
cord-014597-66vd2mdu	1940	47	e.g.	e.g.	RB
cord-014597-66vd2mdu	1940	48	BRCA	BRCA	NNP
cord-014597-66vd2mdu	1940	49	,	,	,
cord-014597-66vd2mdu	1940	50	HAT	HAT	NNP
cord-014597-66vd2mdu	1940	51	)	)	-RRB-
cord-014597-66vd2mdu	1940	52	pathways	pathway	NNS
cord-014597-66vd2mdu	1940	53	.	.	.
cord-014597-66vd2mdu	1941	1	The	the	DT
cord-014597-66vd2mdu	1941	2	mRNA	mRNA	NNP
cord-014597-66vd2mdu	1941	3	levels	level	NNS
cord-014597-66vd2mdu	1941	4	of	of	IN
cord-014597-66vd2mdu	1941	5	Myc	Myc	NNP
cord-014597-66vd2mdu	1941	6	,	,	,
cord-014597-66vd2mdu	1941	7	SMAD	SMAD	NNP
cord-014597-66vd2mdu	1941	8	,	,	,
cord-014597-66vd2mdu	1941	9	Caspase	Caspase	NNP
cord-014597-66vd2mdu	1941	10	,	,	,
cord-014597-66vd2mdu	1941	11	BRCA	BRCA	NNP
cord-014597-66vd2mdu	1941	12	and	and	CC
cord-014597-66vd2mdu	1941	13	HAT	HAT	NNP
cord-014597-66vd2mdu	1941	14	related	relate	VBN
cord-014597-66vd2mdu	1941	15	genes	gene	NNS
cord-014597-66vd2mdu	1941	16	were	be	VBD
cord-014597-66vd2mdu	1941	17	lower	low	JJR
cord-014597-66vd2mdu	1941	18	in	in	IN
cord-014597-66vd2mdu	1941	19	IgG3-SC39	IgG3-SC39	NNP
cord-014597-66vd2mdu	1941	20	,	,	,
cord-014597-66vd2mdu	1941	21	while	while	IN
cord-014597-66vd2mdu	1941	22	those	those	DT
cord-014597-66vd2mdu	1941	23	of	of	IN
cord-014597-66vd2mdu	1941	24	SREBP	SREBP	NNP
cord-014597-66vd2mdu	1941	25	and	and	CC
cord-014597-66vd2mdu	1941	26	PPARγ	PPARγ	NNS
cord-014597-66vd2mdu	1941	27	related	related	JJ
cord-014597-66vd2mdu	1941	28	genes	gene	NNS
cord-014597-66vd2mdu	1941	29	were	be	VBD
cord-014597-66vd2mdu	1941	30	higher	high	JJR
cord-014597-66vd2mdu	1941	31	in	in	IN
cord-014597-66vd2mdu	1941	32	IgG3-SC39	IgG3-SC39	NNP
cord-014597-66vd2mdu	1941	33	.	.	.
cord-014597-66vd2mdu	1942	1	The	the	DT
cord-014597-66vd2mdu	1942	2	effects	effect	NNS
cord-014597-66vd2mdu	1942	3	of	of	IN
cord-014597-66vd2mdu	1942	4	these	these	DT
cord-014597-66vd2mdu	1942	5	pathways	pathway	NNS
cord-014597-66vd2mdu	1942	6	on	on	IN
cord-014597-66vd2mdu	1942	7	antibody	antibody	NN
cord-014597-66vd2mdu	1942	8	production	production	NN
cord-014597-66vd2mdu	1942	9	should	should	MD
cord-014597-66vd2mdu	1942	10	be	be	VB
cord-014597-66vd2mdu	1942	11	examined	examine	VBN
cord-014597-66vd2mdu	1942	12	in	in	IN
cord-014597-66vd2mdu	1942	13	future	future	NN
cord-014597-66vd2mdu	1942	14	.	.	.
cord-014597-66vd2mdu	1943	1	In	in	IN
cord-014597-66vd2mdu	1943	2	this	this	DT
cord-014597-66vd2mdu	1943	3	study	study	NN
cord-014597-66vd2mdu	1943	4	,	,	,
cord-014597-66vd2mdu	1943	5	we	-PRON-	PRP
cord-014597-66vd2mdu	1943	6	found	find	VBD
cord-014597-66vd2mdu	1943	7	that	that	IN
cord-014597-66vd2mdu	1943	8	high	high	JJ
cord-014597-66vd2mdu	1943	9	-	-	HYPH
cord-014597-66vd2mdu	1943	10	chromosome	chromosome	NN
cord-014597-66vd2mdu	1943	11	-	-	HYPH
cord-014597-66vd2mdu	1943	12	number	number	NN
cord-014597-66vd2mdu	1943	13	CHO	CHO	NNP
cord-014597-66vd2mdu	1943	14	cells	cell	NNS
cord-014597-66vd2mdu	1943	15	have	have	VBP
cord-014597-66vd2mdu	1943	16	lower	low	JJR
cord-014597-66vd2mdu	1943	17	amounts	amount	NNS
cord-014597-66vd2mdu	1943	18	of	of	IN
cord-014597-66vd2mdu	1943	19	mRNA	mrna	NN
cord-014597-66vd2mdu	1943	20	relative	relative	JJ
cord-014597-66vd2mdu	1943	21	to	to	IN
cord-014597-66vd2mdu	1943	22	their	-PRON-	PRP$
cord-014597-66vd2mdu	1943	23	volume	volume	NN
cord-014597-66vd2mdu	1943	24	.	.	.
cord-014597-66vd2mdu	1944	1	A	a	DT
cord-014597-66vd2mdu	1944	2	reduction	reduction	NN
cord-014597-66vd2mdu	1944	3	per	per	IN
cord-014597-66vd2mdu	1944	4	unit	unit	NN
cord-014597-66vd2mdu	1944	5	volume	volume	NN
cord-014597-66vd2mdu	1944	6	in	in	IN
cord-014597-66vd2mdu	1944	7	the	the	DT
cord-014597-66vd2mdu	1944	8	expression	expression	NN
cord-014597-66vd2mdu	1944	9	of	of	IN
cord-014597-66vd2mdu	1944	10	genes	gene	NNS
cord-014597-66vd2mdu	1944	11	that	that	WDT
cord-014597-66vd2mdu	1944	12	are	be	VBP
cord-014597-66vd2mdu	1944	13	required	require	VBN
cord-014597-66vd2mdu	1944	14	for	for	IN
cord-014597-66vd2mdu	1944	15	survival	survival	NN
cord-014597-66vd2mdu	1944	16	might	may	MD
cord-014597-66vd2mdu	1944	17	generate	generate	VB
cord-014597-66vd2mdu	1944	18	additional	additional	JJ
cord-014597-66vd2mdu	1944	19	energy	energy	NN
cord-014597-66vd2mdu	1944	20	for	for	IN
cord-014597-66vd2mdu	1944	21	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1944	22	protein	protein	NN
cord-014597-66vd2mdu	1944	23	production	production	NN
cord-014597-66vd2mdu	1944	24	in	in	IN
cord-014597-66vd2mdu	1944	25	high	high	JJ
cord-014597-66vd2mdu	1944	26	-	-	HYPH
cord-014597-66vd2mdu	1944	27	chromosome	chromosome	NN
cord-014597-66vd2mdu	1944	28	-	-	HYPH
cord-014597-66vd2mdu	1944	29	number	number	NN
cord-014597-66vd2mdu	1944	30	cells	cell	NNS
cord-014597-66vd2mdu	1944	31	.	.	.
cord-014597-66vd2mdu	1945	1	From	from	IN
cord-014597-66vd2mdu	1945	2	an	an	DT
cord-014597-66vd2mdu	1945	3	evolutionary	evolutionary	JJ
cord-014597-66vd2mdu	1945	4	perspective	perspective	NN
cord-014597-66vd2mdu	1945	5	,	,	,
cord-014597-66vd2mdu	1945	6	an	an	DT
cord-014597-66vd2mdu	1945	7	increased	increase	VBN
cord-014597-66vd2mdu	1945	8	set	set	NN
cord-014597-66vd2mdu	1945	9	of	of	IN
cord-014597-66vd2mdu	1945	10	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	1945	11	underlies	underlie	VBZ
cord-014597-66vd2mdu	1945	12	rapid	rapid	JJ
cord-014597-66vd2mdu	1945	13	evolutionary	evolutionary	JJ
cord-014597-66vd2mdu	1945	14	adaptation	adaptation	NN
cord-014597-66vd2mdu	1945	15	.	.	.
cord-014597-66vd2mdu	1946	1	Although	although	IN
cord-014597-66vd2mdu	1946	2	there	there	EX
cord-014597-66vd2mdu	1946	3	are	be	VBP
cord-014597-66vd2mdu	1946	4	issues	issue	NNS
cord-014597-66vd2mdu	1946	5	to	to	TO
cord-014597-66vd2mdu	1946	6	be	be	VB
cord-014597-66vd2mdu	1946	7	considered	consider	VBN
cord-014597-66vd2mdu	1946	8	,	,	,
cord-014597-66vd2mdu	1946	9	such	such	JJ
cord-014597-66vd2mdu	1946	10	as	as	IN
cord-014597-66vd2mdu	1946	11	stability	stability	NN
cord-014597-66vd2mdu	1946	12	,	,	,
cord-014597-66vd2mdu	1946	13	there	there	EX
cord-014597-66vd2mdu	1946	14	may	may	MD
cord-014597-66vd2mdu	1946	15	also	also	RB
cord-014597-66vd2mdu	1946	16	be	be	VB
cord-014597-66vd2mdu	1946	17	advantages	advantage	NNS
cord-014597-66vd2mdu	1946	18	to	to	IN
cord-014597-66vd2mdu	1946	19	using	use	VBG
cord-014597-66vd2mdu	1946	20	high	high	JJ
cord-014597-66vd2mdu	1946	21	-	-	HYPH
cord-014597-66vd2mdu	1946	22	chromosome	chromosome	NN
cord-014597-66vd2mdu	1946	23	-	-	HYPH
cord-014597-66vd2mdu	1946	24	number	number	NN
cord-014597-66vd2mdu	1946	25	aneuploid	aneuploid	JJ
cord-014597-66vd2mdu	1946	26	CHO	CHO	NNP
cord-014597-66vd2mdu	1946	27	cells	cell	NNS
cord-014597-66vd2mdu	1946	28	as	as	IN
cord-014597-66vd2mdu	1946	29	a	a	DT
cord-014597-66vd2mdu	1946	30	production	production	NN
cord-014597-66vd2mdu	1946	31	host	host	NN
cord-014597-66vd2mdu	1946	32	cells	cell	NNS
cord-014597-66vd2mdu	1946	33	of	of	IN
cord-014597-66vd2mdu	1946	34	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1946	35	proteins	protein	NNS
cord-014597-66vd2mdu	1946	36	.	.	.
cord-014597-66vd2mdu	1947	1	Background	Background	NNP
cord-014597-66vd2mdu	1947	2	Human	human	JJ
cord-014597-66vd2mdu	1947	3	growth	growth	NN
cord-014597-66vd2mdu	1947	4	factors	factor	NNS
cord-014597-66vd2mdu	1947	5	have	have	VBP
cord-014597-66vd2mdu	1947	6	an	an	DT
cord-014597-66vd2mdu	1947	7	enormous	enormous	JJ
cord-014597-66vd2mdu	1947	8	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1947	9	potential	potential	NN
cord-014597-66vd2mdu	1947	10	.	.	.
cord-014597-66vd2mdu	1948	1	Among	among	IN
cord-014597-66vd2mdu	1948	2	them	-PRON-	PRP
cord-014597-66vd2mdu	1948	3	,	,	,
cord-014597-66vd2mdu	1948	4	the	the	DT
cord-014597-66vd2mdu	1948	5	bone	bone	NN
cord-014597-66vd2mdu	1948	6	morphogenetic	morphogenetic	JJ
cord-014597-66vd2mdu	1948	7	protein-2	protein-2	.
cord-014597-66vd2mdu	1948	8	(	(	-LRB-
cord-014597-66vd2mdu	1948	9	BMP-2	BMP-2	NNP
cord-014597-66vd2mdu	1948	10	)	)	-RRB-
cord-014597-66vd2mdu	1948	11	can	can	MD
cord-014597-66vd2mdu	1948	12	induce	induce	VB
cord-014597-66vd2mdu	1948	13	de	de	FW
cord-014597-66vd2mdu	1948	14	novo	novo	NNP
cord-014597-66vd2mdu	1948	15	bone	bone	NN
cord-014597-66vd2mdu	1948	16	formation	formation	NN
cord-014597-66vd2mdu	1948	17	endowing	endow	VBG
cord-014597-66vd2mdu	1948	18	the	the	DT
cord-014597-66vd2mdu	1948	19	protein	protein	NN
cord-014597-66vd2mdu	1948	20	a	a	DT
cord-014597-66vd2mdu	1948	21	high	high	JJ
cord-014597-66vd2mdu	1948	22	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	1948	23	potential	potential	NN
cord-014597-66vd2mdu	1948	24	.	.	.
cord-014597-66vd2mdu	1949	1	However	however	RB
cord-014597-66vd2mdu	1949	2	,	,	,
cord-014597-66vd2mdu	1949	3	finding	find	VBG
cord-014597-66vd2mdu	1949	4	a	a	DT
cord-014597-66vd2mdu	1949	5	suitable	suitable	JJ
cord-014597-66vd2mdu	1949	6	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1949	7	production	production	NN
cord-014597-66vd2mdu	1949	8	system	system	NN
cord-014597-66vd2mdu	1949	9	for	for	IN
cord-014597-66vd2mdu	1949	10	such	such	PDT
cord-014597-66vd2mdu	1949	11	a	a	DT
cord-014597-66vd2mdu	1949	12	protein	protein	NN
cord-014597-66vd2mdu	1949	13	still	still	RB
cord-014597-66vd2mdu	1949	14	remains	remain	VBZ
cord-014597-66vd2mdu	1949	15	a	a	DT
cord-014597-66vd2mdu	1949	16	challenge	challenge	NN
cord-014597-66vd2mdu	1949	17	.	.	.
cord-014597-66vd2mdu	1950	1	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	1950	2	expression	expression	NN
cord-014597-66vd2mdu	1950	3	of	of	IN
cord-014597-66vd2mdu	1950	4	hBMP2	hbmp2	NN
cord-014597-66vd2mdu	1950	5	was	be	VBD
cord-014597-66vd2mdu	1950	6	investigated	investigate	VBN
cord-014597-66vd2mdu	1950	7	in	in	IN
cord-014597-66vd2mdu	1950	8	transiently	transiently	RB
cord-014597-66vd2mdu	1950	9	transfected	transfecte	VBN
cord-014597-66vd2mdu	1950	10	HEK-293	hek-293	JJ
cord-014597-66vd2mdu	1950	11	cells	cell	NNS
cord-014597-66vd2mdu	1950	12	and	and	CC
cord-014597-66vd2mdu	1950	13	in	in	IN
cord-014597-66vd2mdu	1950	14	stable	stable	JJ
cord-014597-66vd2mdu	1950	15	clones	clone	NNS
cord-014597-66vd2mdu	1950	16	established	establish	VBN
cord-014597-66vd2mdu	1950	17	in	in	IN
cord-014597-66vd2mdu	1950	18	CHO	CHO	NNP
cord-014597-66vd2mdu	1950	19	-	-	HYPH
cord-014597-66vd2mdu	1950	20	K1	K1	NNP
cord-014597-66vd2mdu	1950	21	cells	cell	NNS
cord-014597-66vd2mdu	1950	22	cultivated	cultivate	VBN
cord-014597-66vd2mdu	1950	23	in	in	IN
cord-014597-66vd2mdu	1950	24	ExCell	ExCell	NNP
cord-014597-66vd2mdu	1950	25	and	and	CC
cord-014597-66vd2mdu	1950	26	Pro	pro	JJ
cord-014597-66vd2mdu	1950	27	-	-	JJ
cord-014597-66vd2mdu	1950	28	CHO5	cho5	JJ
cord-014597-66vd2mdu	1950	29	medium	medium	NN
cord-014597-66vd2mdu	1950	30	,	,	,
cord-014597-66vd2mdu	1950	31	respectively	respectively	RB
cord-014597-66vd2mdu	1950	32	.	.	.
cord-014597-66vd2mdu	1951	1	Protein	protein	NN
cord-014597-66vd2mdu	1951	2	stability	stability	NN
cord-014597-66vd2mdu	1951	3	and	and	CC
cord-014597-66vd2mdu	1951	4	interaction	interaction	NN
cord-014597-66vd2mdu	1951	5	of	of	IN
cord-014597-66vd2mdu	1951	6	the	the	DT
cord-014597-66vd2mdu	1951	7	hBMP2	hbmp2	NN
cord-014597-66vd2mdu	1951	8	with	with	IN
cord-014597-66vd2mdu	1951	9	the	the	DT
cord-014597-66vd2mdu	1951	10	producer	producer	NN
cord-014597-66vd2mdu	1951	11	cells	cell	NNS
cord-014597-66vd2mdu	1951	12	were	be	VBD
cord-014597-66vd2mdu	1951	13	investigated	investigate	VBN
cord-014597-66vd2mdu	1951	14	in	in	IN
cord-014597-66vd2mdu	1951	15	vitro	vitro	FW
cord-014597-66vd2mdu	1951	16	using	use	VBG
cord-014597-66vd2mdu	1951	17	commercially	commercially	RB
cord-014597-66vd2mdu	1951	18	available	available	JJ
cord-014597-66vd2mdu	1951	19	rhBMP2	rhbmp2	NN
cord-014597-66vd2mdu	1951	20	.	.	.
cord-014597-66vd2mdu	1952	1	In	in	IN
cord-014597-66vd2mdu	1952	2	addition	addition	NN
cord-014597-66vd2mdu	1952	3	,	,	,
cord-014597-66vd2mdu	1952	4	we	-PRON-	PRP
cord-014597-66vd2mdu	1952	5	investigated	investigate	VBD
cord-014597-66vd2mdu	1952	6	a	a	DT
cord-014597-66vd2mdu	1952	7	cell	cell	NN
cord-014597-66vd2mdu	1952	8	-	-	HYPH
cord-014597-66vd2mdu	1952	9	free	free	JJ
cord-014597-66vd2mdu	1952	10	protein	protein	NN
cord-014597-66vd2mdu	1952	11	synthesis	synthesis	NN
cord-014597-66vd2mdu	1952	12	system	system	NN
cord-014597-66vd2mdu	1952	13	harboring	harbor	VBG
cord-014597-66vd2mdu	1952	14	translocationally	translocationally	RB
cord-014597-66vd2mdu	1952	15	active	active	JJ
cord-014597-66vd2mdu	1952	16	microsomal	microsomal	JJ
cord-014597-66vd2mdu	1952	17	structures	structure	NNS
cord-014597-66vd2mdu	1952	18	,	,	,
cord-014597-66vd2mdu	1952	19	hence	hence	RB
cord-014597-66vd2mdu	1952	20	having	have	VBG
cord-014597-66vd2mdu	1952	21	the	the	DT
cord-014597-66vd2mdu	1952	22	potential	potential	NN
cord-014597-66vd2mdu	1952	23	to	to	TO
cord-014597-66vd2mdu	1952	24	perform	perform	VB
cord-014597-66vd2mdu	1952	25	post	post	JJ
cord-014597-66vd2mdu	1952	26	-	-	JJ
cord-014597-66vd2mdu	1952	27	translational	translational	JJ
cord-014597-66vd2mdu	1952	28	modifications	modification	NNS
cord-014597-66vd2mdu	1952	29	,	,	,
cord-014597-66vd2mdu	1952	30	as	as	IN
cord-014597-66vd2mdu	1952	31	an	an	DT
cord-014597-66vd2mdu	1952	32	alternative	alternative	JJ
cord-014597-66vd2mdu	1952	33	production	production	NN
cord-014597-66vd2mdu	1952	34	method	method	NN
cord-014597-66vd2mdu	1952	35	.	.	.
cord-014597-66vd2mdu	1953	1	We	-PRON-	PRP
cord-014597-66vd2mdu	1953	2	showed	show	VBD
cord-014597-66vd2mdu	1953	3	that	that	IN
cord-014597-66vd2mdu	1953	4	growth	growth	NN
cord-014597-66vd2mdu	1953	5	rates	rate	NNS
cord-014597-66vd2mdu	1953	6	and	and	CC
cord-014597-66vd2mdu	1953	7	viabilities	viability	NNS
cord-014597-66vd2mdu	1953	8	of	of	IN
cord-014597-66vd2mdu	1953	9	the	the	DT
cord-014597-66vd2mdu	1953	10	rhBMP2producing	rhBMP2producing	NNP
cord-014597-66vd2mdu	1953	11	cells	cell	NNS
cord-014597-66vd2mdu	1953	12	were	be	VBD
cord-014597-66vd2mdu	1953	13	similar	similar	JJ
cord-014597-66vd2mdu	1953	14	to	to	IN
cord-014597-66vd2mdu	1953	15	those	those	DT
cord-014597-66vd2mdu	1953	16	of	of	IN
cord-014597-66vd2mdu	1953	17	the	the	DT
cord-014597-66vd2mdu	1953	18	parent	parent	NN
cord-014597-66vd2mdu	1953	19	cell	cell	NN
cord-014597-66vd2mdu	1953	20	line	line	NN
cord-014597-66vd2mdu	1953	21	,	,	,
cord-014597-66vd2mdu	1953	22	while	while	IN
cord-014597-66vd2mdu	1953	23	entry	entry	NN
cord-014597-66vd2mdu	1953	24	into	into	IN
cord-014597-66vd2mdu	1953	25	the	the	DT
cord-014597-66vd2mdu	1953	26	death	death	NN
cord-014597-66vd2mdu	1953	27	phase	phase	NN
cord-014597-66vd2mdu	1953	28	was	be	VBD
cord-014597-66vd2mdu	1953	29	delayed	delay	VBN
cord-014597-66vd2mdu	1953	30	in	in	IN
cord-014597-66vd2mdu	1953	31	case	case	NN
cord-014597-66vd2mdu	1953	32	of	of	IN
cord-014597-66vd2mdu	1953	33	the	the	DT
cord-014597-66vd2mdu	1953	34	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1953	35	cells	cell	NNS
cord-014597-66vd2mdu	1953	36	.	.	.
cord-014597-66vd2mdu	1954	1	The	the	DT
cord-014597-66vd2mdu	1954	2	maximum	maximum	JJ
cord-014597-66vd2mdu	1954	3	rhBMP2	rhbmp2	NN
cord-014597-66vd2mdu	1954	4	concentration	concentration	NN
cord-014597-66vd2mdu	1954	5	detected	detect	VBN
cord-014597-66vd2mdu	1954	6	in	in	IN
cord-014597-66vd2mdu	1954	7	the	the	DT
cord-014597-66vd2mdu	1954	8	culture	culture	NN
cord-014597-66vd2mdu	1954	9	supernatant	supernatant	NN
cord-014597-66vd2mdu	1954	10	was	be	VBD
cord-014597-66vd2mdu	1954	11	low	low	JJ
cord-014597-66vd2mdu	1954	12	for	for	IN
cord-014597-66vd2mdu	1954	13	stable	stable	JJ
cord-014597-66vd2mdu	1954	14	clones	clone	NNS
cord-014597-66vd2mdu	1954	15	but	but	CC
cord-014597-66vd2mdu	1954	16	can	can	MD
cord-014597-66vd2mdu	1954	17	be	be	VB
cord-014597-66vd2mdu	1954	18	greatly	greatly	RB
cord-014597-66vd2mdu	1954	19	improved	improve	VBN
cord-014597-66vd2mdu	1954	20	combining	combine	VBG
cord-014597-66vd2mdu	1954	21	the	the	DT
cord-014597-66vd2mdu	1954	22	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1954	23	cells	cell	NNS
cord-014597-66vd2mdu	1954	24	transient	transient	JJ
cord-014597-66vd2mdu	1954	25	expression	expression	NN
cord-014597-66vd2mdu	1954	26	system	system	NN
cord-014597-66vd2mdu	1954	27	and	and	CC
cord-014597-66vd2mdu	1954	28	batch	batch	NN
cord-014597-66vd2mdu	1954	29	reactor	reactor	NN
cord-014597-66vd2mdu	1954	30	cultivation	cultivation	NN
cord-014597-66vd2mdu	1954	31	which	which	WDT
cord-014597-66vd2mdu	1954	32	reflects	reflect	VBZ
cord-014597-66vd2mdu	1954	33	a	a	DT
cord-014597-66vd2mdu	1954	34	better	well	JJR
cord-014597-66vd2mdu	1954	35	compatibility	compatibility	NN
cord-014597-66vd2mdu	1954	36	of	of	IN
cord-014597-66vd2mdu	1954	37	the	the	DT
cord-014597-66vd2mdu	1954	38	codon	codon	NN
cord-014597-66vd2mdu	1954	39	usage	usage	NN
cord-014597-66vd2mdu	1954	40	in	in	IN
cord-014597-66vd2mdu	1954	41	the	the	DT
cord-014597-66vd2mdu	1954	42	human	human	JJ
cord-014597-66vd2mdu	1954	43	cells	cell	NNS
cord-014597-66vd2mdu	1954	44	(	(	-LRB-
cord-014597-66vd2mdu	1954	45	Table	table	NN
cord-014597-66vd2mdu	1954	46	1	1	CD
cord-014597-66vd2mdu	1954	47	)	)	-RRB-
cord-014597-66vd2mdu	1954	48	.	.	.
cord-014597-66vd2mdu	1955	1	hBMP2	hBMP2	NNP
cord-014597-66vd2mdu	1956	1	protein	protein	NN
cord-014597-66vd2mdu	1956	2	is	be	VBZ
cord-014597-66vd2mdu	1956	3	sensitive	sensitive	JJ
cord-014597-66vd2mdu	1956	4	to	to	IN
cord-014597-66vd2mdu	1956	5	slightly	slightly	RB
cord-014597-66vd2mdu	1956	6	acidic	acidic	JJ
cord-014597-66vd2mdu	1956	7	pH	ph	NN
cord-014597-66vd2mdu	1956	8	and	and	CC
cord-014597-66vd2mdu	1956	9	to	to	IN
cord-014597-66vd2mdu	1956	10	a	a	DT
cord-014597-66vd2mdu	1956	11	lesser	less	JJR
cord-014597-66vd2mdu	1956	12	extend	extend	NN
cord-014597-66vd2mdu	1956	13	to	to	IN
cord-014597-66vd2mdu	1956	14	proteases	protease	NNS
cord-014597-66vd2mdu	1956	15	(	(	-LRB-
cord-014597-66vd2mdu	1956	16	Fig	Fig	NNP
cord-014597-66vd2mdu	1956	17	.	.	.
cord-014597-66vd2mdu	1956	18	1a	1a	CD
cord-014597-66vd2mdu	1956	19	)	)	-RRB-
cord-014597-66vd2mdu	1956	20	and	and	CC
cord-014597-66vd2mdu	1956	21	binds	bind	VBZ
cord-014597-66vd2mdu	1956	22	to	to	IN
cord-014597-66vd2mdu	1956	23	both	both	DT
cord-014597-66vd2mdu	1956	24	producers	producer	NNS
cord-014597-66vd2mdu	1956	25	cell	cell	NN
cord-014597-66vd2mdu	1956	26	lines	line	NNS
cord-014597-66vd2mdu	1956	27	(	(	-LRB-
cord-014597-66vd2mdu	1956	28	Fig	fig	NN
cord-014597-66vd2mdu	1956	29	.	.	.
cord-014597-66vd2mdu	1956	30	1b	1b	CD
cord-014597-66vd2mdu	1956	31	)	)	-RRB-
cord-014597-66vd2mdu	1957	1	-All	-All	:
cord-014597-66vd2mdu	1958	1	this	this	DT
cord-014597-66vd2mdu	1958	2	could	could	MD
cord-014597-66vd2mdu	1958	3	incidentally	incidentally	RB
cord-014597-66vd2mdu	1958	4	contribute	contribute	VB
cord-014597-66vd2mdu	1958	5	to	to	IN
cord-014597-66vd2mdu	1958	6	the	the	DT
cord-014597-66vd2mdu	1958	7	low	low	JJ
cord-014597-66vd2mdu	1958	8	product	product	NN
cord-014597-66vd2mdu	1958	9	titers	titer	NNS
cord-014597-66vd2mdu	1958	10	.	.	.
cord-014597-66vd2mdu	1959	1	Cell	cell	NN
cord-014597-66vd2mdu	1959	2	-	-	HYPH
cord-014597-66vd2mdu	1959	3	free	free	JJ
cord-014597-66vd2mdu	1959	4	protein	protein	NN
cord-014597-66vd2mdu	1959	5	synthesis	synthesis	NN
cord-014597-66vd2mdu	1959	6	has	have	VBZ
cord-014597-66vd2mdu	1959	7	been	be	VBN
cord-014597-66vd2mdu	1959	8	proposed	propose	VBN
cord-014597-66vd2mdu	1959	9	as	as	IN
cord-014597-66vd2mdu	1959	10	alternative	alternative	NN
cord-014597-66vd2mdu	1959	11	for	for	IN
cord-014597-66vd2mdu	1959	12	"	"	``
cord-014597-66vd2mdu	1959	13	difficult	difficult	JJ
cord-014597-66vd2mdu	1959	14	-	-	HYPH
cord-014597-66vd2mdu	1959	15	to	to	TO
cord-014597-66vd2mdu	1959	16	-	-	HYPH
cord-014597-66vd2mdu	1959	17	express	express	VB
cord-014597-66vd2mdu	1959	18	"	"	''
cord-014597-66vd2mdu	1959	19	proteins	protein	NNS
cord-014597-66vd2mdu	1959	20	.	.	.
cord-014597-66vd2mdu	1960	1	Since	since	IN
cord-014597-66vd2mdu	1960	2	native	native	JJ
cord-014597-66vd2mdu	1960	3	hBMP2	hbmp2	NN
cord-014597-66vd2mdu	1960	4	is	be	VBZ
cord-014597-66vd2mdu	1960	5	glycosylated	glycosylate	VBN
cord-014597-66vd2mdu	1960	6	,	,	,
cord-014597-66vd2mdu	1960	7	a	a	DT
cord-014597-66vd2mdu	1960	8	cell	cell	NN
cord-014597-66vd2mdu	1960	9	-	-	HYPH
cord-014597-66vd2mdu	1960	10	free	free	JJ
cord-014597-66vd2mdu	1960	11	system	system	NN
cord-014597-66vd2mdu	1960	12	based	base	VBN
cord-014597-66vd2mdu	1960	13	on	on	IN
cord-014597-66vd2mdu	1960	14	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	1960	15	cell	cell	NN
cord-014597-66vd2mdu	1960	16	lysates	lysate	NNS
cord-014597-66vd2mdu	1960	17	is	be	VBZ
cord-014597-66vd2mdu	1960	18	required	require	VBN
cord-014597-66vd2mdu	1960	19	for	for	IN
cord-014597-66vd2mdu	1960	20	its	-PRON-	PRP$
cord-014597-66vd2mdu	1960	21	production	production	NN
cord-014597-66vd2mdu	1960	22	.	.	.
cord-014597-66vd2mdu	1961	1	CHO	CHO	NNP
cord-014597-66vd2mdu	1961	2	cell	cell	NN
cord-014597-66vd2mdu	1961	3	lysates	lysate	NNS
cord-014597-66vd2mdu	1961	4	were	be	VBD
cord-014597-66vd2mdu	1961	5	chosen	choose	VBN
cord-014597-66vd2mdu	1961	6	,	,	,
cord-014597-66vd2mdu	1961	7	since	since	IN
cord-014597-66vd2mdu	1961	8	they	-PRON-	PRP
cord-014597-66vd2mdu	1961	9	had	have	VBD
cord-014597-66vd2mdu	1961	10	previously	previously	RB
cord-014597-66vd2mdu	1961	11	been	be	VBN
cord-014597-66vd2mdu	1961	12	established	establish	VBN
cord-014597-66vd2mdu	1961	13	as	as	IN
cord-014597-66vd2mdu	1961	14	the	the	DT
cord-014597-66vd2mdu	1961	15	most	most	RBS
cord-014597-66vd2mdu	1961	16	productive	productive	JJ
cord-014597-66vd2mdu	1961	17	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	1961	18	system	system	NN
cord-014597-66vd2mdu	1961	19	in	in	IN
cord-014597-66vd2mdu	1961	20	our	-PRON-	PRP$
cord-014597-66vd2mdu	1961	21	hands	hand	NNS
cord-014597-66vd2mdu	1961	22	[	[	-LRB-
cord-014597-66vd2mdu	1961	23	1	1	CD
cord-014597-66vd2mdu	1961	24	]	]	-RRB-
cord-014597-66vd2mdu	1961	25	,	,	,
cord-014597-66vd2mdu	1961	26	while	while	IN
cord-014597-66vd2mdu	1961	27	concomitantly	concomitantly	RB
cord-014597-66vd2mdu	1961	28	enabling	enable	VBG
cord-014597-66vd2mdu	1961	29	a	a	DT
cord-014597-66vd2mdu	1961	30	direct	direct	JJ
cord-014597-66vd2mdu	1961	31	comparison	comparison	NN
cord-014597-66vd2mdu	1961	32	to	to	IN
cord-014597-66vd2mdu	1961	33	the	the	DT
cord-014597-66vd2mdu	1961	34	production	production	NN
cord-014597-66vd2mdu	1961	35	of	of	IN
cord-014597-66vd2mdu	1961	36	hBMP2	hbmp2	NN
cord-014597-66vd2mdu	1961	37	in	in	IN
cord-014597-66vd2mdu	1961	38	stable	stable	JJ
cord-014597-66vd2mdu	1961	39	clones	clone	NNS
cord-014597-66vd2mdu	1961	40	established	establish	VBN
cord-014597-66vd2mdu	1961	41	in	in	IN
cord-014597-66vd2mdu	1961	42	CHO	CHO	NNP
cord-014597-66vd2mdu	1961	43	-	-	HYPH
cord-014597-66vd2mdu	1961	44	K1	K1	NNP
cord-014597-66vd2mdu	1961	45	.	.	.
cord-014597-66vd2mdu	1962	1	The	the	DT
cord-014597-66vd2mdu	1962	2	ability	ability	NN
cord-014597-66vd2mdu	1962	3	to	to	TO
cord-014597-66vd2mdu	1962	4	perform	perform	VB
cord-014597-66vd2mdu	1962	5	post	post	JJ
cord-014597-66vd2mdu	1962	6	-	-	JJ
cord-014597-66vd2mdu	1962	7	translational	translational	JJ
cord-014597-66vd2mdu	1962	8	modifications	modification	NNS
cord-014597-66vd2mdu	1962	9	is	be	VBZ
cord-014597-66vd2mdu	1962	10	a	a	DT
cord-014597-66vd2mdu	1962	11	major	major	JJ
cord-014597-66vd2mdu	1962	12	advantage	advantage	NN
cord-014597-66vd2mdu	1962	13	of	of	IN
cord-014597-66vd2mdu	1962	14	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	1962	15	systems	system	NNS
cord-014597-66vd2mdu	1962	16	.	.	.
cord-014597-66vd2mdu	1963	1	The	the	DT
cord-014597-66vd2mdu	1963	2	CHO	CHO	NNP
cord-014597-66vd2mdu	1963	3	lysates	lysate	NNS
cord-014597-66vd2mdu	1963	4	prepared	prepare	VBN
cord-014597-66vd2mdu	1963	5	by	by	IN
cord-014597-66vd2mdu	1963	6	the	the	DT
cord-014597-66vd2mdu	1963	7	protocol	protocol	NN
cord-014597-66vd2mdu	1963	8	used	use	VBN
cord-014597-66vd2mdu	1963	9	here	here	RB
cord-014597-66vd2mdu	1963	10	have	have	VBP
cord-014597-66vd2mdu	1963	11	previously	previously	RB
cord-014597-66vd2mdu	1963	12	been	be	VBN
cord-014597-66vd2mdu	1963	13	shown	show	VBN
cord-014597-66vd2mdu	1963	14	to	to	TO
cord-014597-66vd2mdu	1963	15	contain	contain	VB
cord-014597-66vd2mdu	1963	16	significant	significant	JJ
cord-014597-66vd2mdu	1963	17	amounts	amount	NNS
cord-014597-66vd2mdu	1963	18	of	of	IN
cord-014597-66vd2mdu	1963	19	endogenous	endogenous	JJ
cord-014597-66vd2mdu	1963	20	microsomes	microsome	NNS
cord-014597-66vd2mdu	1963	21	derived	derive	VBN
cord-014597-66vd2mdu	1963	22	from	from	IN
cord-014597-66vd2mdu	1963	23	the	the	DT
cord-014597-66vd2mdu	1963	24	endoplasmatic	endoplasmatic	JJ
cord-014597-66vd2mdu	1963	25	reticulum	reticulum	NN
cord-014597-66vd2mdu	1963	26	during	during	IN
cord-014597-66vd2mdu	1963	27	lysis	lysis	NN
cord-014597-66vd2mdu	1963	28	[	[	-LRB-
cord-014597-66vd2mdu	1963	29	2	2	CD
cord-014597-66vd2mdu	1963	30	]	]	-RRB-
cord-014597-66vd2mdu	1963	31	.	.	.
cord-014597-66vd2mdu	1964	1	To	to	TO
cord-014597-66vd2mdu	1964	2	enforce	enforce	VB
cord-014597-66vd2mdu	1964	3	translocation	translocation	NN
cord-014597-66vd2mdu	1964	4	of	of	IN
cord-014597-66vd2mdu	1964	5	the	the	DT
cord-014597-66vd2mdu	1964	6	target	target	NN
cord-014597-66vd2mdu	1964	7	protein	protein	NN
cord-014597-66vd2mdu	1964	8	into	into	IN
cord-014597-66vd2mdu	1964	9	the	the	DT
cord-014597-66vd2mdu	1964	10	microsomal	microsomal	NNP
cord-014597-66vd2mdu	1964	11	structures	structure	NNS
cord-014597-66vd2mdu	1964	12	,	,	,
cord-014597-66vd2mdu	1964	13	a	a	DT
cord-014597-66vd2mdu	1964	14	melittin	melittin	NNP
cord-014597-66vd2mdu	1964	15	signal	signal	NN
cord-014597-66vd2mdu	1964	16	peptide	peptide	NN
cord-014597-66vd2mdu	1964	17	was	be	VBD
cord-014597-66vd2mdu	1964	18	fused	fuse	VBN
cord-014597-66vd2mdu	1964	19	to	to	IN
cord-014597-66vd2mdu	1964	20	the	the	DT
cord-014597-66vd2mdu	1964	21	hBMP2	hbmp2	NN
cord-014597-66vd2mdu	1964	22	cDNA	cdna	NN
cord-014597-66vd2mdu	1964	23	.	.	.
cord-014597-66vd2mdu	1965	1	The	the	DT
cord-014597-66vd2mdu	1965	2	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	1965	3	of	of	IN
cord-014597-66vd2mdu	1965	4	the	the	DT
cord-014597-66vd2mdu	1965	5	protein	protein	NN
cord-014597-66vd2mdu	1965	6	was	be	VBD
cord-014597-66vd2mdu	1965	7	assessed	assess	VBN
cord-014597-66vd2mdu	1965	8	by	by	IN
cord-014597-66vd2mdu	1965	9	enzymatic	enzymatic	JJ
cord-014597-66vd2mdu	1965	10	treatment	treatment	NN
cord-014597-66vd2mdu	1965	11	(	(	-LRB-
cord-014597-66vd2mdu	1965	12	PNGase	PNGase	NNP
cord-014597-66vd2mdu	1965	13	,	,	,
cord-014597-66vd2mdu	1965	14	EndoH	EndoH	NNP
cord-014597-66vd2mdu	1965	15	)	)	-RRB-
cord-014597-66vd2mdu	1965	16	and	and	CC
cord-014597-66vd2mdu	1965	17	confirmed	confirm	VBD
cord-014597-66vd2mdu	1965	18	using	use	VBG
cord-014597-66vd2mdu	1965	19	14	14	CD
cord-014597-66vd2mdu	1965	20	C	c	NN
cord-014597-66vd2mdu	1965	21	-	-	HYPH
cord-014597-66vd2mdu	1965	22	mannose	mannose	NN
cord-014597-66vd2mdu	1965	23	for	for	IN
cord-014597-66vd2mdu	1965	24	the	the	DT
cord-014597-66vd2mdu	1965	25	de	de	NNP
cord-014597-66vd2mdu	1965	26	novo	novo	NNP
cord-014597-66vd2mdu	1965	27	protein	protein	NNP
cord-014597-66vd2mdu	1965	28	synthesis	synthesis	NN
cord-014597-66vd2mdu	1965	29	.	.	.
cord-014597-66vd2mdu	1966	1	Upon	upon	IN
cord-014597-66vd2mdu	1966	2	cell	cell	NN
cord-014597-66vd2mdu	1966	3	-	-	HYPH
cord-014597-66vd2mdu	1966	4	free	free	JJ
cord-014597-66vd2mdu	1966	5	protein	protein	NN
cord-014597-66vd2mdu	1966	6	synthesis	synthesis	NN
cord-014597-66vd2mdu	1966	7	,	,	,
cord-014597-66vd2mdu	1966	8	the	the	DT
cord-014597-66vd2mdu	1966	9	hBMP2	hbmp2	NN
cord-014597-66vd2mdu	1966	10	yield	yield	NN
cord-014597-66vd2mdu	1966	11	was	be	VBD
cord-014597-66vd2mdu	1966	12	100-fold	100-fold	CD
cord-014597-66vd2mdu	1966	13	higher	high	JJR
cord-014597-66vd2mdu	1966	14	than	than	IN
cord-014597-66vd2mdu	1966	15	the	the	DT
cord-014597-66vd2mdu	1966	16	best	good	JJS
cord-014597-66vd2mdu	1966	17	one	one	CD
cord-014597-66vd2mdu	1966	18	in	in	IN
cord-014597-66vd2mdu	1966	19	the	the	DT
cord-014597-66vd2mdu	1966	20	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	1966	21	cells	cell	NNS
cord-014597-66vd2mdu	1966	22	.	.	.
cord-014597-66vd2mdu	1967	1	The	the	DT
cord-014597-66vd2mdu	1967	2	difference	difference	NN
cord-014597-66vd2mdu	1967	3	becomes	become	VBZ
cord-014597-66vd2mdu	1967	4	even	even	RB
cord-014597-66vd2mdu	1967	5	more	more	RBR
cord-014597-66vd2mdu	1967	6	dramatic	dramatic	JJ
cord-014597-66vd2mdu	1967	7	,	,	,
cord-014597-66vd2mdu	1967	8	when	when	WRB
cord-014597-66vd2mdu	1967	9	productivities	productivity	NNS
cord-014597-66vd2mdu	1967	10	are	be	VBP
cord-014597-66vd2mdu	1967	11	considered	consider	VBN
cord-014597-66vd2mdu	1967	12	(	(	-LRB-
cord-014597-66vd2mdu	1967	13	Table	table	NN
cord-014597-66vd2mdu	1967	14	1	1	CD
cord-014597-66vd2mdu	1967	15	)	)	-RRB-
cord-014597-66vd2mdu	1967	16	,	,	,
cord-014597-66vd2mdu	1967	17	i.e.	i.e.	FW
cord-014597-66vd2mdu	1967	18	the	the	DT
cord-014597-66vd2mdu	1967	19	fact	fact	NN
cord-014597-66vd2mdu	1967	20	that	that	IN
cord-014597-66vd2mdu	1967	21	maximum	maximum	JJ
cord-014597-66vd2mdu	1967	22	product	product	NN
cord-014597-66vd2mdu	1967	23	titers	titer	NNS
cord-014597-66vd2mdu	1967	24	are	be	VBP
cord-014597-66vd2mdu	1967	25	reached	reach	VBN
cord-014597-66vd2mdu	1967	26	within	within	IN
cord-014597-66vd2mdu	1967	27	3	3	CD
cord-014597-66vd2mdu	1967	28	h	h	NN
cord-014597-66vd2mdu	1967	29	in	in	IN
cord-014597-66vd2mdu	1967	30	the	the	DT
cord-014597-66vd2mdu	1967	31	cellfree	cellfree	NNP
cord-014597-66vd2mdu	1967	32	system	system	NN
cord-014597-66vd2mdu	1967	33	compared	compare	VBN
cord-014597-66vd2mdu	1967	34	to	to	IN
cord-014597-66vd2mdu	1967	35	120	120	CD
cord-014597-66vd2mdu	1967	36	h	h	NN
cord-014597-66vd2mdu	1967	37	in	in	IN
cord-014597-66vd2mdu	1967	38	the	the	DT
cord-014597-66vd2mdu	1967	39	cell	cell	NN
cord-014597-66vd2mdu	1967	40	-	-	HYPH
cord-014597-66vd2mdu	1967	41	based	base	VBN
cord-014597-66vd2mdu	1967	42	ones	one	NNS
cord-014597-66vd2mdu	1967	43	.	.	.
cord-014597-66vd2mdu	1968	1	This	this	DT
cord-014597-66vd2mdu	1968	2	demonstrates	demonstrate	VBZ
cord-014597-66vd2mdu	1968	3	that	that	IN
cord-014597-66vd2mdu	1968	4	the	the	DT
cord-014597-66vd2mdu	1968	5	cell	cell	NN
cord-014597-66vd2mdu	1968	6	-	-	HYPH
cord-014597-66vd2mdu	1968	7	free	free	JJ
cord-014597-66vd2mdu	1968	8	expression	expression	NN
cord-014597-66vd2mdu	1968	9	system	system	NN
cord-014597-66vd2mdu	1968	10	is	be	VBZ
cord-014597-66vd2mdu	1968	11	most	most	RBS
cord-014597-66vd2mdu	1968	12	suitable	suitable	JJ
cord-014597-66vd2mdu	1968	13	compared	compare	VBN
cord-014597-66vd2mdu	1968	14	to	to	IN
cord-014597-66vd2mdu	1968	15	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1968	16	cell	cell	NN
cord-014597-66vd2mdu	1968	17	expression	expression	NN
cord-014597-66vd2mdu	1968	18	method	method	NN
cord-014597-66vd2mdu	1968	19	for	for	IN
cord-014597-66vd2mdu	1968	20	the	the	DT
cord-014597-66vd2mdu	1968	21	production	production	NN
cord-014597-66vd2mdu	1968	22	of	of	IN
cord-014597-66vd2mdu	1968	23	glycosylated	glycosylated	JJ
cord-014597-66vd2mdu	1968	24	human	human	JJ
cord-014597-66vd2mdu	1968	25	BMP2	BMP2	NNP
cord-014597-66vd2mdu	1968	26	(	(	-LRB-
cord-014597-66vd2mdu	1968	27	Table	table	NN
cord-014597-66vd2mdu	1968	28	1	1	CD
cord-014597-66vd2mdu	1968	29	)	)	-RRB-
cord-014597-66vd2mdu	1968	30	[	[	-LRB-
cord-014597-66vd2mdu	1968	31	3	3	CD
cord-014597-66vd2mdu	1968	32	]	]	-RRB-
cord-014597-66vd2mdu	1968	33	.	.	.
cord-014597-66vd2mdu	1969	1	Human	human	JJ
cord-014597-66vd2mdu	1969	2	growth	growth	NN
cord-014597-66vd2mdu	1969	3	factors	factor	NNS
cord-014597-66vd2mdu	1969	4	are	be	VBP
cord-014597-66vd2mdu	1969	5	complex	complex	JJ
cord-014597-66vd2mdu	1969	6	molecules	molecule	NNS
cord-014597-66vd2mdu	1969	7	,	,	,
cord-014597-66vd2mdu	1969	8	which	which	WDT
cord-014597-66vd2mdu	1969	9	make	make	VBP
cord-014597-66vd2mdu	1969	10	their	-PRON-	PRP$
cord-014597-66vd2mdu	1969	11	production	production	NN
cord-014597-66vd2mdu	1969	12	in	in	IN
cord-014597-66vd2mdu	1969	13	mammalian	mammalian	JJ
cord-014597-66vd2mdu	1969	14	cells	cell	NNS
cord-014597-66vd2mdu	1969	15	desirable	desirable	JJ
cord-014597-66vd2mdu	1969	16	.	.	.
cord-014597-66vd2mdu	1970	1	However	however	RB
cord-014597-66vd2mdu	1970	2	,	,	,
cord-014597-66vd2mdu	1970	3	low	low	JJ
cord-014597-66vd2mdu	1970	4	product	product	NN
cord-014597-66vd2mdu	1970	5	titers	titer	NNS
cord-014597-66vd2mdu	1970	6	caused	cause	VBN
cord-014597-66vd2mdu	1970	7	by	by	IN
cord-014597-66vd2mdu	1970	8	a	a	DT
cord-014597-66vd2mdu	1970	9	variety	variety	NN
cord-014597-66vd2mdu	1970	10	of	of	IN
cord-014597-66vd2mdu	1970	11	both	both	DT
cord-014597-66vd2mdu	1970	12	cell	cell	NN
cord-014597-66vd2mdu	1970	13	and	and	CC
cord-014597-66vd2mdu	1970	14	process	process	NN
cord-014597-66vd2mdu	1970	15	related	relate	VBN
cord-014597-66vd2mdu	1970	16	effects	effect	NNS
cord-014597-66vd2mdu	1970	17	may	may	MD
cord-014597-66vd2mdu	1970	18	hinder	hinder	VB
cord-014597-66vd2mdu	1970	19	the	the	DT
cord-014597-66vd2mdu	1970	20	development	development	NN
cord-014597-66vd2mdu	1970	21	of	of	IN
cord-014597-66vd2mdu	1970	22	highly	highly	RB
cord-014597-66vd2mdu	1970	23	productive	productive	JJ
cord-014597-66vd2mdu	1970	24	processes	process	NNS
cord-014597-66vd2mdu	1970	25	.	.	.
cord-014597-66vd2mdu	1971	1	In	in	IN
cord-014597-66vd2mdu	1971	2	such	such	JJ
cord-014597-66vd2mdu	1971	3	cases	case	NNS
cord-014597-66vd2mdu	1971	4	,	,	,
cord-014597-66vd2mdu	1971	5	cell	cell	NN
cord-014597-66vd2mdu	1971	6	-	-	HYPH
cord-014597-66vd2mdu	1971	7	free	free	JJ
cord-014597-66vd2mdu	1971	8	protein	protein	NN
cord-014597-66vd2mdu	1971	9	production	production	NN
cord-014597-66vd2mdu	1971	10	using	use	VBG
cord-014597-66vd2mdu	1971	11	CHO	CHO	NNP
cord-014597-66vd2mdu	1971	12	cell	cell	NN
cord-014597-66vd2mdu	1971	13	lysates	lysate	NNS
cord-014597-66vd2mdu	1971	14	containing	contain	VBG
cord-014597-66vd2mdu	1971	15	endogenous	endogenous	JJ
cord-014597-66vd2mdu	1971	16	microsomes	microsome	NNS
cord-014597-66vd2mdu	1971	17	for	for	IN
cord-014597-66vd2mdu	1971	18	posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	1971	19	processing	processing	NN
cord-014597-66vd2mdu	1971	20	,	,	,
cord-014597-66vd2mdu	1971	21	may	may	MD
cord-014597-66vd2mdu	1971	22	eventually	eventually	RB
cord-014597-66vd2mdu	1971	23	present	present	VB
cord-014597-66vd2mdu	1971	24	an	an	DT
cord-014597-66vd2mdu	1971	25	attractive	attractive	JJ
cord-014597-66vd2mdu	1971	26	alternative	alternative	NN
cord-014597-66vd2mdu	1971	27	.	.	.
cord-014597-66vd2mdu	1972	1	In	in	IN
cord-014597-66vd2mdu	1972	2	particular	particular	JJ
cord-014597-66vd2mdu	1972	3	since	since	IN
cord-014597-66vd2mdu	1972	4	these	these	DT
cord-014597-66vd2mdu	1972	5	lysates	lysate	NNS
cord-014597-66vd2mdu	1972	6	can	can	MD
cord-014597-66vd2mdu	1972	7	be	be	VB
cord-014597-66vd2mdu	1972	8	used	use	VBN
cord-014597-66vd2mdu	1972	9	under	under	IN
cord-014597-66vd2mdu	1972	10	tightly	tightly	RB
cord-014597-66vd2mdu	1972	11	controlled	control	VBN
cord-014597-66vd2mdu	1972	12	conditions	condition	NNS
cord-014597-66vd2mdu	1972	13	assuring	assure	VBG
cord-014597-66vd2mdu	1972	14	a	a	DT
cord-014597-66vd2mdu	1972	15	higher	high	JJR
cord-014597-66vd2mdu	1972	16	degree	degree	NN
cord-014597-66vd2mdu	1972	17	of	of	IN
cord-014597-66vd2mdu	1972	18	reproducibility	reproducibility	NN
cord-014597-66vd2mdu	1972	19	,	,	,
cord-014597-66vd2mdu	1972	20	than	than	IN
cord-014597-66vd2mdu	1972	21	,	,	,
cord-014597-66vd2mdu	1972	22	e.g.	e.g.	IN
cord-014597-66vd2mdu	1972	23	transient	transient	JJ
cord-014597-66vd2mdu	1972	24	transfection	transfection	NN
cord-014597-66vd2mdu	1972	25	systems	system	NNS
cord-014597-66vd2mdu	1972	26	.	.	.
cord-014597-66vd2mdu	1973	1	Cell	cell	NN
cord-014597-66vd2mdu	1973	2	-	-	HYPH
cord-014597-66vd2mdu	1973	3	free	free	JJ
cord-014597-66vd2mdu	1973	4	systems	system	NNS
cord-014597-66vd2mdu	1973	5	are	be	VBP
cord-014597-66vd2mdu	1973	6	known	know	VBN
cord-014597-66vd2mdu	1973	7	to	to	TO
cord-014597-66vd2mdu	1973	8	circumvent	circumvent	VB
cord-014597-66vd2mdu	1973	9	typical	typical	JJ
cord-014597-66vd2mdu	1973	10	bottlenecks	bottleneck	NNS
cord-014597-66vd2mdu	1973	11	of	of	IN
cord-014597-66vd2mdu	1973	12	cellbased	cellbased	JJ
cord-014597-66vd2mdu	1973	13	ones	one	NNS
cord-014597-66vd2mdu	1973	14	,	,	,
cord-014597-66vd2mdu	1973	15	e.g.	e.g.	DT
cord-014597-66vd2mdu	1973	16	metabolic	metabolic	JJ
cord-014597-66vd2mdu	1973	17	regulation	regulation	NN
cord-014597-66vd2mdu	1973	18	and	and	CC
cord-014597-66vd2mdu	1973	19	cell	cell	NN
cord-014597-66vd2mdu	1973	20	maintenance	maintenance	NN
cord-014597-66vd2mdu	1973	21	mechanisms	mechanism	NNS
cord-014597-66vd2mdu	1973	22	.	.	.
cord-014597-66vd2mdu	1974	1	In	in	IN
cord-014597-66vd2mdu	1974	2	consequence	consequence	NN
cord-014597-66vd2mdu	1974	3	,	,	,
cord-014597-66vd2mdu	1974	4	the	the	DT
cord-014597-66vd2mdu	1974	5	production	production	NN
cord-014597-66vd2mdu	1974	6	of	of	IN
cord-014597-66vd2mdu	1974	7	a	a	DT
cord-014597-66vd2mdu	1974	8	recombinant	recombinant	JJ
cord-014597-66vd2mdu	1974	9	protein	protein	NN
cord-014597-66vd2mdu	1974	10	is	be	VBZ
cord-014597-66vd2mdu	1974	11	neither	neither	CC
cord-014597-66vd2mdu	1974	12	inhibited	inhibit	VBN
cord-014597-66vd2mdu	1974	13	by	by	IN
cord-014597-66vd2mdu	1974	14	its	-PRON-	PRP$
cord-014597-66vd2mdu	1974	15	accumulation	accumulation	NN
cord-014597-66vd2mdu	1974	16	nor	nor	CC
cord-014597-66vd2mdu	1974	17	by	by	IN
cord-014597-66vd2mdu	1974	18	any	any	DT
cord-014597-66vd2mdu	1974	19	interaction	interaction	NN
cord-014597-66vd2mdu	1974	20	with	with	IN
cord-014597-66vd2mdu	1974	21	the	the	DT
cord-014597-66vd2mdu	1974	22	cells	cell	NNS
cord-014597-66vd2mdu	1974	23	,	,	,
cord-014597-66vd2mdu	1974	24	e.g.	e.g.	RB
cord-014597-66vd2mdu	1974	25	through	through	IN
cord-014597-66vd2mdu	1974	26	the	the	DT
cord-014597-66vd2mdu	1974	27	activation	activation	NN
cord-014597-66vd2mdu	1974	28	of	of	IN
cord-014597-66vd2mdu	1974	29	inhibitory	inhibitory	JJ
cord-014597-66vd2mdu	1974	30	signaling	signal	VBG
cord-014597-66vd2mdu	1974	31	pathways	pathway	NNS
cord-014597-66vd2mdu	1974	32	.	.	.
cord-014597-66vd2mdu	1975	1	core	core	NN
cord-014597-66vd2mdu	1975	2	.	.	.
cord-014597-66vd2mdu	1976	1	Preliminary	preliminary	JJ
cord-014597-66vd2mdu	1976	2	studies	study	NNS
cord-014597-66vd2mdu	1976	3	showed	show	VBD
cord-014597-66vd2mdu	1976	4	that	that	IN
cord-014597-66vd2mdu	1976	5	the	the	DT
cord-014597-66vd2mdu	1976	6	corresponding	correspond	VBG
cord-014597-66vd2mdu	1976	7	polyplexes	polyplexes	NN
cord-014597-66vd2mdu	1976	8	,	,	,
cord-014597-66vd2mdu	1976	9	but	but	CC
cord-014597-66vd2mdu	1976	10	also	also	RB
cord-014597-66vd2mdu	1976	11	some	some	DT
cord-014597-66vd2mdu	1976	12	of	of	IN
cord-014597-66vd2mdu	1976	13	the	the	DT
cord-014597-66vd2mdu	1976	14	cells	cell	NNS
cord-014597-66vd2mdu	1976	15	that	that	WDT
cord-014597-66vd2mdu	1976	16	came	come	VBD
cord-014597-66vd2mdu	1976	17	into	into	IN
cord-014597-66vd2mdu	1976	18	contact	contact	NN
cord-014597-66vd2mdu	1976	19	with	with	IN
cord-014597-66vd2mdu	1976	20	them	-PRON-	PRP
cord-014597-66vd2mdu	1976	21	,	,	,
cord-014597-66vd2mdu	1976	22	became	become	VBD
cord-014597-66vd2mdu	1976	23	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1976	24	and	and	CC
cord-014597-66vd2mdu	1976	25	were	be	VBD
cord-014597-66vd2mdu	1976	26	manageable	manageable	JJ
cord-014597-66vd2mdu	1976	27	by	by	IN
cord-014597-66vd2mdu	1976	28	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1976	29	fields	field	NNS
cord-014597-66vd2mdu	1976	30	[	[	-LRB-
cord-014597-66vd2mdu	1976	31	1	1	CD
cord-014597-66vd2mdu	1976	32	]	]	-RRB-
cord-014597-66vd2mdu	1976	33	[	[	-LRB-
cord-014597-66vd2mdu	1976	34	2	2	CD
cord-014597-66vd2mdu	1976	35	]	]	-RRB-
cord-014597-66vd2mdu	1976	36	[	[	-LRB-
cord-014597-66vd2mdu	1976	37	3	3	CD
cord-014597-66vd2mdu	1976	38	]	]	-RRB-
cord-014597-66vd2mdu	1976	39	.	.	.
cord-014597-66vd2mdu	1977	1	Here	here	RB
cord-014597-66vd2mdu	1977	2	,	,	,
cord-014597-66vd2mdu	1977	3	we	-PRON-	PRP
cord-014597-66vd2mdu	1977	4	present	present	VBP
cord-014597-66vd2mdu	1977	5	a	a	DT
cord-014597-66vd2mdu	1977	6	characterization	characterization	NN
cord-014597-66vd2mdu	1977	7	of	of	IN
cord-014597-66vd2mdu	1977	8	the	the	DT
cord-014597-66vd2mdu	1977	9	influence	influence	NN
cord-014597-66vd2mdu	1977	10	of	of	IN
cord-014597-66vd2mdu	1977	11	structure	structure	NN
cord-014597-66vd2mdu	1977	12	and	and	CC
cord-014597-66vd2mdu	1977	13	composition	composition	NN
cord-014597-66vd2mdu	1977	14	on	on	IN
cord-014597-66vd2mdu	1977	15	the	the	DT
cord-014597-66vd2mdu	1977	16	function	function	NN
cord-014597-66vd2mdu	1977	17	of	of	IN
cord-014597-66vd2mdu	1977	18	these	these	DT
cord-014597-66vd2mdu	1977	19	polymers	polymer	NNS
cord-014597-66vd2mdu	1977	20	using	use	VBG
cord-014597-66vd2mdu	1977	21	a	a	DT
cord-014597-66vd2mdu	1977	22	library	library	NN
cord-014597-66vd2mdu	1977	23	of	of	IN
cord-014597-66vd2mdu	1977	24	highly	highly	RB
cord-014597-66vd2mdu	1977	25	homogeneous	homogeneous	JJ
cord-014597-66vd2mdu	1977	26	,	,	,
cord-014597-66vd2mdu	1977	27	paramagnetic	paramagnetic	JJ
cord-014597-66vd2mdu	1977	28	nano	nano	NN
cord-014597-66vd2mdu	1977	29	-	-	HYPH
cord-014597-66vd2mdu	1977	30	stars	star	NNS
cord-014597-66vd2mdu	1977	31	with	with	IN
cord-014597-66vd2mdu	1977	32	varied	varied	JJ
cord-014597-66vd2mdu	1977	33	arm	arm	NN
cord-014597-66vd2mdu	1977	34	lengths	length	NNS
cord-014597-66vd2mdu	1977	35	and	and	CC
cord-014597-66vd2mdu	1977	36	densities	density	NNS
cord-014597-66vd2mdu	1977	37	[	[	-LRB-
cord-014597-66vd2mdu	1977	38	4	4	CD
cord-014597-66vd2mdu	1977	39	]	]	-RRB-
cord-014597-66vd2mdu	1977	40	.	.	.
cord-014597-66vd2mdu	1978	1	The	the	DT
cord-014597-66vd2mdu	1978	2	paramagnetic	paramagnetic	JJ
cord-014597-66vd2mdu	1978	3	nano	nano	NN
cord-014597-66vd2mdu	1978	4	-	-	HYPH
cord-014597-66vd2mdu	1978	5	stars	star	NNS
cord-014597-66vd2mdu	1978	6	library	library	NN
cord-014597-66vd2mdu	1978	7	was	be	VBD
cord-014597-66vd2mdu	1978	8	synthesized	synthesize	VBN
cord-014597-66vd2mdu	1978	9	by	by	IN
cord-014597-66vd2mdu	1978	10	coating	coat	VBG
cord-014597-66vd2mdu	1978	11	maghemite	maghemite	VBN
cord-014597-66vd2mdu	1978	12	nanoparticles	nanoparticle	NNS
cord-014597-66vd2mdu	1978	13	(	(	-LRB-
cord-014597-66vd2mdu	1978	14	γ	γ	NN
cord-014597-66vd2mdu	1978	15	-	-	HYPH
cord-014597-66vd2mdu	1978	16	Fe	Fe	NNP
cord-014597-66vd2mdu	1978	17	2	2	CD
cord-014597-66vd2mdu	1978	18	O	o	NN
cord-014597-66vd2mdu	1978	19	3	3	CD
cord-014597-66vd2mdu	1978	20	)	)	-RRB-
cord-014597-66vd2mdu	1978	21	with	with	IN
cord-014597-66vd2mdu	1978	22	a	a	DT
cord-014597-66vd2mdu	1978	23	thin	thin	JJ
cord-014597-66vd2mdu	1978	24	silica	silica	NN
cord-014597-66vd2mdu	1978	25	-	-	HYPH
cord-014597-66vd2mdu	1978	26	shell	shell	NN
cord-014597-66vd2mdu	1978	27	functionalized	functionalize	VBN
cord-014597-66vd2mdu	1978	28	with	with	IN
cord-014597-66vd2mdu	1978	29	an	an	DT
cord-014597-66vd2mdu	1978	30	atomic	atomic	JJ
cord-014597-66vd2mdu	1978	31	transfer	transfer	NN
cord-014597-66vd2mdu	1978	32	radical	radical	JJ
cord-014597-66vd2mdu	1978	33	polymerization	polymerization	NN
cord-014597-66vd2mdu	1978	34	(	(	-LRB-
cord-014597-66vd2mdu	1978	35	ATRP	ATRP	NNP
cord-014597-66vd2mdu	1978	36	)	)	-RRB-
cord-014597-66vd2mdu	1978	37	initiator	initiator	NN
cord-014597-66vd2mdu	1978	38	.	.	.
cord-014597-66vd2mdu	1979	1	PDMAEMA	PDMAEMA	NNP
cord-014597-66vd2mdu	1979	2	arms	arm	NNS
cord-014597-66vd2mdu	1979	3	were	be	VBD
cord-014597-66vd2mdu	1979	4	grown	grow	VBN
cord-014597-66vd2mdu	1979	5	from	from	IN
cord-014597-66vd2mdu	1979	6	the	the	DT
cord-014597-66vd2mdu	1979	7	core	core	NN
cord-014597-66vd2mdu	1979	8	particles	particle	NNS
cord-014597-66vd2mdu	1979	9	via	via	IN
cord-014597-66vd2mdu	1979	10	ATRP	ATRP	NNP
cord-014597-66vd2mdu	1979	11	.	.	.
cord-014597-66vd2mdu	1980	1	In	in	IN
cord-014597-66vd2mdu	1980	2	one	one	CD
cord-014597-66vd2mdu	1980	3	case	case	NN
cord-014597-66vd2mdu	1980	4	,	,	,
cord-014597-66vd2mdu	1980	5	the	the	DT
cord-014597-66vd2mdu	1980	6	PDMAEMA	PDMAEMA	NNP
cord-014597-66vd2mdu	1980	7	arms	arm	NNS
cord-014597-66vd2mdu	1980	8	was	be	VBD
cord-014597-66vd2mdu	1980	9	end	end	NN
cord-014597-66vd2mdu	1980	10	-	-	HYPH
cord-014597-66vd2mdu	1980	11	capped	cap	VBN
cord-014597-66vd2mdu	1980	12	with	with	IN
cord-014597-66vd2mdu	1980	13	PDEGMA	PDEGMA	NNP
cord-014597-66vd2mdu	1980	14	blocks	block	NNS
cord-014597-66vd2mdu	1980	15	produced	produce	VBN
cord-014597-66vd2mdu	1980	16	during	during	IN
cord-014597-66vd2mdu	1980	17	a	a	DT
cord-014597-66vd2mdu	1980	18	second	second	JJ
cord-014597-66vd2mdu	1980	19	ATRP	ATRP	NNP
cord-014597-66vd2mdu	1980	20	step	step	NN
cord-014597-66vd2mdu	1980	21	.	.	.
cord-014597-66vd2mdu	1981	1	All	all	DT
cord-014597-66vd2mdu	1981	2	nanostars	nanostar	NNS
cord-014597-66vd2mdu	1981	3	were	be	VBD
cord-014597-66vd2mdu	1981	4	characterized	characterize	VBN
cord-014597-66vd2mdu	1981	5	by	by	IN
cord-014597-66vd2mdu	1981	6	size	size	NN
cord-014597-66vd2mdu	1981	7	exclusion	exclusion	NN
cord-014597-66vd2mdu	1981	8	chromatography	chromatography	NN
cord-014597-66vd2mdu	1981	9	and	and	CC
cord-014597-66vd2mdu	1981	10	thermogravimetry	thermogravimetry	NNP
cord-014597-66vd2mdu	1981	11	to	to	TO
cord-014597-66vd2mdu	1981	12	calculate	calculate	VB
cord-014597-66vd2mdu	1981	13	number	number	NN
cord-014597-66vd2mdu	1981	14	and	and	CC
cord-014597-66vd2mdu	1981	15	length	length	NN
cord-014597-66vd2mdu	1981	16	of	of	IN
cord-014597-66vd2mdu	1981	17	the	the	DT
cord-014597-66vd2mdu	1981	18	PDMAEMA	PDMAEMA	NNP
cord-014597-66vd2mdu	1981	19	arms	arm	NNS
cord-014597-66vd2mdu	1981	20	.	.	.
cord-014597-66vd2mdu	1982	1	The	the	DT
cord-014597-66vd2mdu	1982	2	core	core	NN
cord-014597-66vd2mdu	1982	3	diameter	diameter	NN
cord-014597-66vd2mdu	1982	4	was	be	VBD
cord-014597-66vd2mdu	1982	5	determined	determine	VBN
cord-014597-66vd2mdu	1982	6	by	by	IN
cord-014597-66vd2mdu	1982	7	transmission	transmission	NN
cord-014597-66vd2mdu	1982	8	electron	electron	NN
cord-014597-66vd2mdu	1982	9	microscopy	microscopy	NN
cord-014597-66vd2mdu	1982	10	and	and	CC
cord-014597-66vd2mdu	1982	11	dynamic	dynamic	JJ
cord-014597-66vd2mdu	1982	12	light	light	JJ
cord-014597-66vd2mdu	1982	13	scattering	scattering	NN
cord-014597-66vd2mdu	1982	14	(	(	-LRB-
cord-014597-66vd2mdu	1982	15	DLS	DLS	NNP
cord-014597-66vd2mdu	1982	16	)	)	-RRB-
cord-014597-66vd2mdu	1982	17	.	.	.
cord-014597-66vd2mdu	1983	1	The	the	DT
cord-014597-66vd2mdu	1983	2	different	different	JJ
cord-014597-66vd2mdu	1983	3	variants	variant	NNS
cord-014597-66vd2mdu	1983	4	(	(	-LRB-
cord-014597-66vd2mdu	1983	5	Table	table	NN
cord-014597-66vd2mdu	1983	6	1	1	CD
cord-014597-66vd2mdu	1983	7	)	)	-RRB-
cord-014597-66vd2mdu	1983	8	were	be	VBD
cord-014597-66vd2mdu	1983	9	analyzed	analyze	VBN
cord-014597-66vd2mdu	1983	10	for	for	IN
cord-014597-66vd2mdu	1983	11	their	-PRON-	PRP$
cord-014597-66vd2mdu	1983	12	ability	ability	NN
cord-014597-66vd2mdu	1983	13	to	to	TO
cord-014597-66vd2mdu	1983	14	complex	complex	VB
cord-014597-66vd2mdu	1983	15	pDNA	pdna	RB
cord-014597-66vd2mdu	1983	16	(	(	-LRB-
cord-014597-66vd2mdu	1983	17	pEGFP	pEGFP	NNP
cord-014597-66vd2mdu	1983	18	-	-	HYPH
cord-014597-66vd2mdu	1983	19	N1	N1	NNP
cord-014597-66vd2mdu	1983	20	)	)	-RRB-
cord-014597-66vd2mdu	1983	21	using	use	VBG
cord-014597-66vd2mdu	1983	22	various	various	JJ
cord-014597-66vd2mdu	1983	23	physicochemical	physicochemical	JJ
cord-014597-66vd2mdu	1983	24	methods	method	NNS
cord-014597-66vd2mdu	1983	25	(	(	-LRB-
cord-014597-66vd2mdu	1983	26	DLS	DLS	NNP
cord-014597-66vd2mdu	1983	27	,	,	,
cord-014597-66vd2mdu	1983	28	Zeta	Zeta	NNP
cord-014597-66vd2mdu	1983	29	sizer	sizer	NN
cord-014597-66vd2mdu	1983	30	)	)	-RRB-
cord-014597-66vd2mdu	1983	31	.	.	.
cord-014597-66vd2mdu	1984	1	Transfection	transfection	NN
cord-014597-66vd2mdu	1984	2	efficiency	efficiency	NN
cord-014597-66vd2mdu	1984	3	/	/	SYM
cord-014597-66vd2mdu	1984	4	cytotoxicity	cytotoxicity	NN
cord-014597-66vd2mdu	1984	5	in	in	IN
cord-014597-66vd2mdu	1984	6	CHO	CHO	NNP
cord-014597-66vd2mdu	1984	7	-	-	HYPH
cord-014597-66vd2mdu	1984	8	K1	K1	NNP
cord-014597-66vd2mdu	1984	9	cells	cell	NNS
cord-014597-66vd2mdu	1984	10	were	be	VBD
cord-014597-66vd2mdu	1984	11	determined	determine	VBN
cord-014597-66vd2mdu	1984	12	by	by	IN
cord-014597-66vd2mdu	1984	13	flow	flow	NN
cord-014597-66vd2mdu	1984	14	cytometry	cytometry	NN
cord-014597-66vd2mdu	1984	15	.	.	.
cord-014597-66vd2mdu	1985	1	Transfected	transfected	JJ
cord-014597-66vd2mdu	1985	2	cells	cell	NNS
cord-014597-66vd2mdu	1985	3	were	be	VBD
cord-014597-66vd2mdu	1985	4	placed	place	VBN
cord-014597-66vd2mdu	1985	5	in	in	IN
cord-014597-66vd2mdu	1985	6	a	a	DT
cord-014597-66vd2mdu	1985	7	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1985	8	field	field	NN
cord-014597-66vd2mdu	1985	9	and	and	CC
cord-014597-66vd2mdu	1985	10	the	the	DT
cord-014597-66vd2mdu	1985	11	influence	influence	NN
cord-014597-66vd2mdu	1985	12	of	of	IN
cord-014597-66vd2mdu	1985	13	the	the	DT
cord-014597-66vd2mdu	1985	14	polymer	polymer	NN
cord-014597-66vd2mdu	1985	15	architecture	architecture	NN
cord-014597-66vd2mdu	1985	16	on	on	IN
cord-014597-66vd2mdu	1985	17	the	the	DT
cord-014597-66vd2mdu	1985	18	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1985	19	separation	separation	NN
cord-014597-66vd2mdu	1985	20	was	be	VBD
cord-014597-66vd2mdu	1985	21	investigated	investigate	VBN
cord-014597-66vd2mdu	1985	22	.	.	.
cord-014597-66vd2mdu	1986	1	Nonparametric	Nonparametric	NNP
cord-014597-66vd2mdu	1986	2	Spearman	Spearman	NNP
cord-014597-66vd2mdu	1986	3	analysis	analysis	NN
cord-014597-66vd2mdu	1986	4	was	be	VBD
cord-014597-66vd2mdu	1986	5	used	use	VBN
cord-014597-66vd2mdu	1986	6	to	to	TO
cord-014597-66vd2mdu	1986	7	correlate	correlate	VB
cord-014597-66vd2mdu	1986	8	between	between	IN
cord-014597-66vd2mdu	1986	9	arm	arm	NN
cord-014597-66vd2mdu	1986	10	length	length	NN
cord-014597-66vd2mdu	1986	11	/	/	SYM
cord-014597-66vd2mdu	1986	12	arm	arm	NN
cord-014597-66vd2mdu	1986	13	densities	density	NNS
cord-014597-66vd2mdu	1986	14	,	,	,
cord-014597-66vd2mdu	1986	15	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1986	16	properties	property	NNS
cord-014597-66vd2mdu	1986	17	of	of	IN
cord-014597-66vd2mdu	1986	18	the	the	DT
cord-014597-66vd2mdu	1986	19	cells	cell	NNS
cord-014597-66vd2mdu	1986	20	and	and	CC
cord-014597-66vd2mdu	1986	21	transfection	transfection	NN
cord-014597-66vd2mdu	1986	22	efficiency	efficiency	NN
cord-014597-66vd2mdu	1986	23	.	.	.
cord-014597-66vd2mdu	1987	1	Based	base	VBN
cord-014597-66vd2mdu	1987	2	on	on	IN
cord-014597-66vd2mdu	1987	3	the	the	DT
cord-014597-66vd2mdu	1987	4	hydrodynamic	hydrodynamic	JJ
cord-014597-66vd2mdu	1987	5	radii	radius	NNS
cord-014597-66vd2mdu	1987	6	of	of	IN
cord-014597-66vd2mdu	1987	7	the	the	DT
cord-014597-66vd2mdu	1987	8	polyplexes	polyplexe	NNS
cord-014597-66vd2mdu	1987	9	,	,	,
cord-014597-66vd2mdu	1987	10	the	the	DT
cord-014597-66vd2mdu	1987	11	investigated	investigate	VBN
cord-014597-66vd2mdu	1987	12	nano	nano	NN
cord-014597-66vd2mdu	1987	13	-	-	HYPH
cord-014597-66vd2mdu	1987	14	stars	star	NNS
cord-014597-66vd2mdu	1987	15	could	could	MD
cord-014597-66vd2mdu	1987	16	be	be	VB
cord-014597-66vd2mdu	1987	17	divided	divide	VBN
cord-014597-66vd2mdu	1987	18	into	into	IN
cord-014597-66vd2mdu	1987	19	three	three	CD
cord-014597-66vd2mdu	1987	20	subgroups	subgroup	NNS
cord-014597-66vd2mdu	1987	21	(	(	-LRB-
cord-014597-66vd2mdu	1987	22	Table	table	NN
cord-014597-66vd2mdu	1987	23	1	1	CD
cord-014597-66vd2mdu	1987	24	)	)	-RRB-
cord-014597-66vd2mdu	1987	25	.	.	.
cord-014597-66vd2mdu	1988	1	Middle	middle	JJ
cord-014597-66vd2mdu	1988	2	,	,	,
cord-014597-66vd2mdu	1988	3	but	but	CC
cord-014597-66vd2mdu	1988	4	also	also	RB
cord-014597-66vd2mdu	1988	5	high	high	JJ
cord-014597-66vd2mdu	1988	6	arm	arm	NN
cord-014597-66vd2mdu	1988	7	density	density	NN
cord-014597-66vd2mdu	1988	8	nano	nano	NN
cord-014597-66vd2mdu	1988	9	-	-	HYPH
cord-014597-66vd2mdu	1988	10	stars	star	NNS
cord-014597-66vd2mdu	1988	11	formed	form	VBD
cord-014597-66vd2mdu	1988	12	smaller	small	JJR
cord-014597-66vd2mdu	1988	13	polyplexes	polyplexe	NNS
cord-014597-66vd2mdu	1988	14	with	with	IN
cord-014597-66vd2mdu	1988	15	hydrodynamic	hydrodynamic	JJ
cord-014597-66vd2mdu	1988	16	radii	radius	NNS
cord-014597-66vd2mdu	1988	17	≤	≤	NNP
cord-014597-66vd2mdu	1988	18	300	300	CD
cord-014597-66vd2mdu	1988	19	nm	nm	NN
cord-014597-66vd2mdu	1988	20	,	,	,
cord-014597-66vd2mdu	1988	21	a	a	DT
cord-014597-66vd2mdu	1988	22	size	size	NN
cord-014597-66vd2mdu	1988	23	that	that	WDT
cord-014597-66vd2mdu	1988	24	is	be	VBZ
cord-014597-66vd2mdu	1988	25	considered	consider	VBN
cord-014597-66vd2mdu	1988	26	suitable	suitable	JJ
cord-014597-66vd2mdu	1988	27	for	for	IN
cord-014597-66vd2mdu	1988	28	endocytosis	endocytosis	NN
cord-014597-66vd2mdu	1988	29	and	and	CC
cord-014597-66vd2mdu	1988	30	transfection	transfection	NN
cord-014597-66vd2mdu	1988	31	.	.	.
cord-014597-66vd2mdu	1989	1	Transfection	transfection	NN
cord-014597-66vd2mdu	1989	2	efficiencies	efficiency	NNS
cord-014597-66vd2mdu	1989	3	and	and	CC
cord-014597-66vd2mdu	1989	4	cytotoxicities	cytotoxicity	NNS
cord-014597-66vd2mdu	1989	5	varied	vary	VBN
cord-014597-66vd2mdu	1989	6	systematically	systematically	RB
cord-014597-66vd2mdu	1989	7	with	with	IN
cord-014597-66vd2mdu	1989	8	the	the	DT
cord-014597-66vd2mdu	1989	9	nano	nano	NN
cord-014597-66vd2mdu	1989	10	-	-	HYPH
cord-014597-66vd2mdu	1989	11	stars	star	NNS
cord-014597-66vd2mdu	1989	12	architecture	architecture	NN
cord-014597-66vd2mdu	1989	13	,	,	,
cord-014597-66vd2mdu	1989	14	with	with	IN
cord-014597-66vd2mdu	1989	15	viability	viability	NN
cord-014597-66vd2mdu	1989	16	showing	show	VBG
cord-014597-66vd2mdu	1989	17	a	a	DT
cord-014597-66vd2mdu	1989	18	more	more	RBR
cord-014597-66vd2mdu	1989	19	pronounced	pronounced	JJ
cord-014597-66vd2mdu	1989	20	dependency	dependency	NN
cord-014597-66vd2mdu	1989	21	on	on	IN
cord-014597-66vd2mdu	1989	22	the	the	DT
cord-014597-66vd2mdu	1989	23	characteristics	characteristic	NNS
cord-014597-66vd2mdu	1989	24	of	of	IN
cord-014597-66vd2mdu	1989	25	the	the	DT
cord-014597-66vd2mdu	1989	26	transfection	transfection	NN
cord-014597-66vd2mdu	1989	27	agent	agent	NN
cord-014597-66vd2mdu	1989	28	than	than	IN
cord-014597-66vd2mdu	1989	29	the	the	DT
cord-014597-66vd2mdu	1989	30	transfection	transfection	NN
cord-014597-66vd2mdu	1989	31	efficiency	efficiency	NN
cord-014597-66vd2mdu	1989	32	itself	-PRON-	PRP
cord-014597-66vd2mdu	1989	33	.	.	.
cord-014597-66vd2mdu	1990	1	The	the	DT
cord-014597-66vd2mdu	1990	2	arm	arm	NN
cord-014597-66vd2mdu	1990	3	density	density	NN
cord-014597-66vd2mdu	1990	4	was	be	VBD
cord-014597-66vd2mdu	1990	5	particularly	particularly	RB
cord-014597-66vd2mdu	1990	6	important	important	JJ
cord-014597-66vd2mdu	1990	7	,	,	,
cord-014597-66vd2mdu	1990	8	with	with	IN
cord-014597-66vd2mdu	1990	9	values	value	NNS
cord-014597-66vd2mdu	1990	10	of	of	IN
cord-014597-66vd2mdu	1990	11	approximately	approximately	RB
cord-014597-66vd2mdu	1990	12	0.06	0.06	CD
cord-014597-66vd2mdu	1990	13	arms/	arms/	CD
cord-014597-66vd2mdu	1990	14	nm	nm	NN
cord-014597-66vd2mdu	1990	15	2	2	CD
cord-014597-66vd2mdu	1990	16	yielding	yield	VBG
cord-014597-66vd2mdu	1990	17	the	the	DT
cord-014597-66vd2mdu	1990	18	best	good	JJS
cord-014597-66vd2mdu	1990	19	results	result	NNS
cord-014597-66vd2mdu	1990	20	(	(	-LRB-
cord-014597-66vd2mdu	1990	21	Fig	fig	NN
cord-014597-66vd2mdu	1990	22	.	.	.
cord-014597-66vd2mdu	1990	23	1a	1a	CD
cord-014597-66vd2mdu	1990	24	)	)	-RRB-
cord-014597-66vd2mdu	1990	25	.	.	.
cord-014597-66vd2mdu	1991	1	The	the	DT
cord-014597-66vd2mdu	1991	2	end	end	NN
cord-014597-66vd2mdu	1991	3	-	-	HYPH
cord-014597-66vd2mdu	1991	4	capping	cap	VBG
cord-014597-66vd2mdu	1991	5	the	the	DT
cord-014597-66vd2mdu	1991	6	polycation	polycation	NN
cord-014597-66vd2mdu	1991	7	arms	arm	NNS
cord-014597-66vd2mdu	1991	8	with	with	IN
cord-014597-66vd2mdu	1991	9	PDEGMA	PDEGMA	NNP
cord-014597-66vd2mdu	1991	10	significantly	significantly	RB
cord-014597-66vd2mdu	1991	11	improved	improve	VBD
cord-014597-66vd2mdu	1991	12	the	the	DT
cord-014597-66vd2mdu	1991	13	serum	serum	NN
cord-014597-66vd2mdu	1991	14	compatibility	compatibility	NN
cord-014597-66vd2mdu	1991	15	(	(	-LRB-
cord-014597-66vd2mdu	1991	16	Fig	fig	NN
cord-014597-66vd2mdu	1991	17	.	.	.
cord-014597-66vd2mdu	1991	18	1b	1b	CD
cord-014597-66vd2mdu	1991	19	)	)	-RRB-
cord-014597-66vd2mdu	1991	20	.	.	.
cord-014597-66vd2mdu	1992	1	The	the	DT
cord-014597-66vd2mdu	1992	2	gene	gene	NN
cord-014597-66vd2mdu	1992	3	delivery	delivery	NN
cord-014597-66vd2mdu	1992	4	potential	potential	NN
cord-014597-66vd2mdu	1992	5	of	of	IN
cord-014597-66vd2mdu	1992	6	a	a	DT
cord-014597-66vd2mdu	1992	7	given	give	VBN
cord-014597-66vd2mdu	1992	8	nano	nano	NN
cord-014597-66vd2mdu	1992	9	-	-	HYPH
cord-014597-66vd2mdu	1992	10	star	star	NN
cord-014597-66vd2mdu	1992	11	and	and	CC
cord-014597-66vd2mdu	1992	12	its	-PRON-	PRP$
cord-014597-66vd2mdu	1992	13	ability	ability	NN
cord-014597-66vd2mdu	1992	14	to	to	TO
cord-014597-66vd2mdu	1992	15	render	render	VB
cord-014597-66vd2mdu	1992	16	the	the	DT
cord-014597-66vd2mdu	1992	17	cells	cell	NNS
cord-014597-66vd2mdu	1992	18	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1992	19	did	do	VBD
cord-014597-66vd2mdu	1992	20	not	not	RB
cord-014597-66vd2mdu	1992	21	correlate	correlate	VB
cord-014597-66vd2mdu	1992	22	.	.	.
cord-014597-66vd2mdu	1993	1	Although	although	IN
cord-014597-66vd2mdu	1993	2	,	,	,
cord-014597-66vd2mdu	1993	3	compared	compare	VBN
cord-014597-66vd2mdu	1993	4	to	to	IN
cord-014597-66vd2mdu	1993	5	the	the	DT
cord-014597-66vd2mdu	1993	6	non	non	JJ
cord-014597-66vd2mdu	1993	7	-	-	JJ
cord-014597-66vd2mdu	1993	8	separated	separated	JJ
cord-014597-66vd2mdu	1993	9	cells	cell	NNS
cord-014597-66vd2mdu	1993	10	,	,	,
cord-014597-66vd2mdu	1993	11	EGFP	EGFP	NNP
cord-014597-66vd2mdu	1993	12	-	-	HYPH
cord-014597-66vd2mdu	1993	13	expressing	express	VBG
cord-014597-66vd2mdu	1993	14	cells	cell	NNS
cord-014597-66vd2mdu	1993	15	were	be	VBD
cord-014597-66vd2mdu	1993	16	consistently	consistently	RB
cord-014597-66vd2mdu	1993	17	more	more	RBR
cord-014597-66vd2mdu	1993	18	frequent	frequent	JJ
cord-014597-66vd2mdu	1993	19	in	in	IN
cord-014597-66vd2mdu	1993	20	the	the	DT
cord-014597-66vd2mdu	1993	21	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1993	22	cell	cell	NN
cord-014597-66vd2mdu	1993	23	fraction	fraction	NN
cord-014597-66vd2mdu	1993	24	,	,	,
cord-014597-66vd2mdu	1993	25	while	while	IN
cord-014597-66vd2mdu	1993	26	the	the	DT
cord-014597-66vd2mdu	1993	27	non	non	JJ
cord-014597-66vd2mdu	1993	28	-	-	JJ
cord-014597-66vd2mdu	1993	29	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1993	30	fraction	fraction	NN
cord-014597-66vd2mdu	1993	31	was	be	VBD
cord-014597-66vd2mdu	1993	32	slightly	slightly	RB
cord-014597-66vd2mdu	1993	33	depleted	deplete	VBN
cord-014597-66vd2mdu	1993	34	.	.	.
cord-014597-66vd2mdu	1994	1	When	when	WRB
cord-014597-66vd2mdu	1994	2	the	the	DT
cord-014597-66vd2mdu	1994	3	EGFP	EGFP	NNP
cord-014597-66vd2mdu	1994	4	-	-	HYPH
cord-014597-66vd2mdu	1994	5	expressing	express	VBG
cord-014597-66vd2mdu	1994	6	cells	cell	NNS
cord-014597-66vd2mdu	1994	7	were	be	VBD
cord-014597-66vd2mdu	1994	8	further	further	RB
cord-014597-66vd2mdu	1994	9	divided	divide	VBN
cord-014597-66vd2mdu	1994	10	into	into	IN
cord-014597-66vd2mdu	1994	11	low	low	JJ
cord-014597-66vd2mdu	1994	12	,	,	,
cord-014597-66vd2mdu	1994	13	middle	middle	JJ
cord-014597-66vd2mdu	1994	14	and	and	CC
cord-014597-66vd2mdu	1994	15	high	high	JJ
cord-014597-66vd2mdu	1994	16	producers	producer	NNS
cord-014597-66vd2mdu	1994	17	,	,	,
cord-014597-66vd2mdu	1994	18	a	a	DT
cord-014597-66vd2mdu	1994	19	statistically	statistically	RB
cord-014597-66vd2mdu	1994	20	significant	significant	JJ
cord-014597-66vd2mdu	1994	21	shift	shift	NN
cord-014597-66vd2mdu	1994	22	towards	towards	IN
cord-014597-66vd2mdu	1994	23	the	the	DT
cord-014597-66vd2mdu	1994	24	high	high	JJ
cord-014597-66vd2mdu	1994	25	producers	producer	NNS
cord-014597-66vd2mdu	1994	26	was	be	VBD
cord-014597-66vd2mdu	1994	27	observed	observe	VBN
cord-014597-66vd2mdu	1994	28	in	in	IN
cord-014597-66vd2mdu	1994	29	the	the	DT
cord-014597-66vd2mdu	1994	30	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1994	31	cell	cell	NN
cord-014597-66vd2mdu	1994	32	fraction	fraction	NN
cord-014597-66vd2mdu	1994	33	(	(	-LRB-
cord-014597-66vd2mdu	1994	34	Fig	Fig	NNP
cord-014597-66vd2mdu	1994	35	.	.	.
cord-014597-66vd2mdu	1994	36	1c	1c	CD
cord-014597-66vd2mdu	1994	37	)	)	-RRB-
cord-014597-66vd2mdu	1994	38	.	.	.
cord-014597-66vd2mdu	1995	1	A	a	DT
cord-014597-66vd2mdu	1995	2	nonparametric	nonparametric	JJ
cord-014597-66vd2mdu	1995	3	Spearman	Spearman	NNP
cord-014597-66vd2mdu	1995	4	correlation	correlation	NN
cord-014597-66vd2mdu	1995	5	analysis	analysis	NN
cord-014597-66vd2mdu	1995	6	was	be	VBD
cord-014597-66vd2mdu	1995	7	used	use	VBN
cord-014597-66vd2mdu	1995	8	to	to	TO
cord-014597-66vd2mdu	1995	9	statistically	statistically	RB
cord-014597-66vd2mdu	1995	10	evaluate	evaluate	VB
cord-014597-66vd2mdu	1995	11	possible	possible	JJ
cord-014597-66vd2mdu	1995	12	links	link	NNS
cord-014597-66vd2mdu	1995	13	between	between	IN
cord-014597-66vd2mdu	1995	14	the	the	DT
cord-014597-66vd2mdu	1995	15	molecular	molecular	JJ
cord-014597-66vd2mdu	1995	16	characteristics	characteristic	NNS
cord-014597-66vd2mdu	1995	17	of	of	IN
cord-014597-66vd2mdu	1995	18	the	the	DT
cord-014597-66vd2mdu	1995	19	nano	nano	NN
cord-014597-66vd2mdu	1995	20	-	-	HYPH
cord-014597-66vd2mdu	1995	21	stars	star	NNS
cord-014597-66vd2mdu	1995	22	,	,	,
cord-014597-66vd2mdu	1995	23	the	the	DT
cord-014597-66vd2mdu	1995	24	physicochemical	physicochemical	JJ
cord-014597-66vd2mdu	1995	25	properties	property	NNS
cord-014597-66vd2mdu	1995	26	of	of	IN
cord-014597-66vd2mdu	1995	27	the	the	DT
cord-014597-66vd2mdu	1995	28	corresponding	correspond	VBG
cord-014597-66vd2mdu	1995	29	polyplexes	polyplexe	NNS
cord-014597-66vd2mdu	1995	30	,	,	,
cord-014597-66vd2mdu	1995	31	the	the	DT
cord-014597-66vd2mdu	1995	32	transfection	transfection	NN
cord-014597-66vd2mdu	1995	33	conditions	condition	NNS
cord-014597-66vd2mdu	1995	34	,	,	,
cord-014597-66vd2mdu	1995	35	and	and	CC
cord-014597-66vd2mdu	1995	36	the	the	DT
cord-014597-66vd2mdu	1995	37	cellular	cellular	JJ
cord-014597-66vd2mdu	1995	38	reactions	reaction	NNS
cord-014597-66vd2mdu	1995	39	.	.	.
cord-014597-66vd2mdu	1996	1	The	the	DT
cord-014597-66vd2mdu	1996	2	resulting	result	VBG
cord-014597-66vd2mdu	1996	3	correlogram	correlogram	NN
cord-014597-66vd2mdu	1996	4	is	be	VBZ
cord-014597-66vd2mdu	1996	5	shown	show	VBN
cord-014597-66vd2mdu	1996	6	in	in	IN
cord-014597-66vd2mdu	1996	7	Fig	Fig	NNP
cord-014597-66vd2mdu	1996	8	.	.	.
cord-014597-66vd2mdu	1997	1	1d	1d	NNP
cord-014597-66vd2mdu	1997	2	.	.	.
cord-014597-66vd2mdu	1998	1	Transfection	transfection	NN
cord-014597-66vd2mdu	1998	2	agents	agent	NNS
cord-014597-66vd2mdu	1998	3	with	with	IN
cord-014597-66vd2mdu	1998	4	magnetic	magnetic	JJ
cord-014597-66vd2mdu	1998	5	properties	property	NNS
cord-014597-66vd2mdu	1998	6	enlarge	enlarge	VBP
cord-014597-66vd2mdu	1998	7	the	the	DT
cord-014597-66vd2mdu	1998	8	toolbox	toolbox	NN
cord-014597-66vd2mdu	1998	9	for	for	IN
cord-014597-66vd2mdu	1998	10	studying	study	VBG
cord-014597-66vd2mdu	1998	11	non	non	JJ
cord-014597-66vd2mdu	1998	12	-	-	JJ
cord-014597-66vd2mdu	1998	13	viral	viral	JJ
cord-014597-66vd2mdu	1998	14	gene	gene	NN
cord-014597-66vd2mdu	1998	15	delivery	delivery	NN
cord-014597-66vd2mdu	1998	16	,	,	,
cord-014597-66vd2mdu	1998	17	since	since	IN
cord-014597-66vd2mdu	1998	18	cellular	cellular	JJ
cord-014597-66vd2mdu	1998	19	magnetism	magnetism	NN
cord-014597-66vd2mdu	1998	20	is	be	VBZ
cord-014597-66vd2mdu	1998	21	added	add	VBN
cord-014597-66vd2mdu	1998	22	as	as	IN
cord-014597-66vd2mdu	1998	23	a	a	DT
cord-014597-66vd2mdu	1998	24	new	new	JJ
cord-014597-66vd2mdu	1998	25	parameter	parameter	NN
cord-014597-66vd2mdu	1998	26	.	.	.
cord-014597-66vd2mdu	1999	1	This	this	DT
cord-014597-66vd2mdu	1999	2	allows	allow	VBZ
cord-014597-66vd2mdu	1999	3	,	,	,
cord-014597-66vd2mdu	1999	4	inter	inter	NNP
cord-014597-66vd2mdu	1999	5	alia	alia	NNP
cord-014597-66vd2mdu	1999	6	,	,	,
cord-014597-66vd2mdu	1999	7	a	a	DT
cord-014597-66vd2mdu	1999	8	distinction	distinction	NN
cord-014597-66vd2mdu	1999	9	between	between	IN
cord-014597-66vd2mdu	1999	10	mere	mere	JJ
cord-014597-66vd2mdu	1999	11	cellular	cellular	JJ
cord-014597-66vd2mdu	1999	12	interaction	interaction	NN
cord-014597-66vd2mdu	1999	13	and	and	CC
cord-014597-66vd2mdu	1999	14	actual	actual	JJ
cord-014597-66vd2mdu	1999	15	uptake	uptake	NN
cord-014597-66vd2mdu	1999	16	,	,	,
cord-014597-66vd2mdu	1999	17	which	which	WDT
cord-014597-66vd2mdu	1999	18	is	be	VBZ
cord-014597-66vd2mdu	1999	19	otherwise	otherwise	RB
cord-014597-66vd2mdu	1999	20	difficult	difficult	JJ
cord-014597-66vd2mdu	1999	21	.	.	.
cord-014597-66vd2mdu	2000	1	Viability	viability	NN
cord-014597-66vd2mdu	2000	2	showed	show	VBD
cord-014597-66vd2mdu	2000	3	a	a	DT
cord-014597-66vd2mdu	2000	4	much	much	RB
cord-014597-66vd2mdu	2000	5	more	more	RBR
cord-014597-66vd2mdu	2000	6	pronounced	pronounced	JJ
cord-014597-66vd2mdu	2000	7	dependency	dependency	NN
cord-014597-66vd2mdu	2000	8	on	on	IN
cord-014597-66vd2mdu	2000	9	the	the	DT
cord-014597-66vd2mdu	2000	10	characteristics	characteristic	NNS
cord-014597-66vd2mdu	2000	11	of	of	IN
cord-014597-66vd2mdu	2000	12	the	the	DT
cord-014597-66vd2mdu	2000	13	transfection	transfection	NN
cord-014597-66vd2mdu	2000	14	agent	agent	NN
cord-014597-66vd2mdu	2000	15	/	/	SYM
cord-014597-66vd2mdu	2000	16	polyplex	polyplex	NN
cord-014597-66vd2mdu	2000	17	than	than	IN
cord-014597-66vd2mdu	2000	18	the	the	DT
cord-014597-66vd2mdu	2000	19	transfection	transfection	NN
cord-014597-66vd2mdu	2000	20	efficiency	efficiency	NN
cord-014597-66vd2mdu	2000	21	itself	-PRON-	PRP
cord-014597-66vd2mdu	2000	22	,	,	,
cord-014597-66vd2mdu	2000	23	which	which	WDT
cord-014597-66vd2mdu	2000	24	should	should	MD
cord-014597-66vd2mdu	2000	25	be	be	VB
cord-014597-66vd2mdu	2000	26	taken	take	VBN
cord-014597-66vd2mdu	2000	27	into	into	IN
cord-014597-66vd2mdu	2000	28	account	account	NN
cord-014597-66vd2mdu	2000	29	during	during	IN
cord-014597-66vd2mdu	2000	30	method	method	NN
cord-014597-66vd2mdu	2000	31	optimization	optimization	NN
cord-014597-66vd2mdu	2000	32	.	.	.
cord-014597-66vd2mdu	2001	1	End	end	NN
cord-014597-66vd2mdu	2001	2	-	-	HYPH
cord-014597-66vd2mdu	2001	3	capping	cap	VBG
cord-014597-66vd2mdu	2001	4	the	the	DT
cord-014597-66vd2mdu	2001	5	polycationic	polycationic	NNP
cord-014597-66vd2mdu	2001	6	PDMAEMA	PDMAEMA	NNP
cord-014597-66vd2mdu	2001	7	-	-	HYPH
cord-014597-66vd2mdu	2001	8	arms	arm	NNS
cord-014597-66vd2mdu	2001	9	with	with	IN
cord-014597-66vd2mdu	2001	10	PDEGMA	PDEGMA	NNP
cord-014597-66vd2mdu	2001	11	-	-	HYPH
cord-014597-66vd2mdu	2001	12	blocks	block	NNS
cord-014597-66vd2mdu	2001	13	improved	improve	VBD
cord-014597-66vd2mdu	2001	14	the	the	DT
cord-014597-66vd2mdu	2001	15	compatibility	compatibility	NN
cord-014597-66vd2mdu	2001	16	of	of	IN
cord-014597-66vd2mdu	2001	17	the	the	DT
cord-014597-66vd2mdu	2001	18	polycationic	polycationic	NNP
cord-014597-66vd2mdu	2001	19	nano	nano	NNP
cord-014597-66vd2mdu	2001	20	-	-	HYPH
cord-014597-66vd2mdu	2001	21	stars	star	NNS
cord-014597-66vd2mdu	2001	22	with	with	IN
cord-014597-66vd2mdu	2001	23	serum	serum	NN
cord-014597-66vd2mdu	2001	24	components	component	NNS
cord-014597-66vd2mdu	2001	25	.	.	.
cord-014597-66vd2mdu	2002	1	In	in	IN
cord-014597-66vd2mdu	2002	2	future	future	NN
cord-014597-66vd2mdu	2002	3	optimized	optimize	VBN
cord-014597-66vd2mdu	2002	4	,	,	,
cord-014597-66vd2mdu	2002	5	blood	blood	NN
cord-014597-66vd2mdu	2002	6	-	-	HYPH
cord-014597-66vd2mdu	2002	7	compatible	compatible	JJ
cord-014597-66vd2mdu	2002	8	,	,	,
cord-014597-66vd2mdu	2002	9	nano	nano	NN
cord-014597-66vd2mdu	2002	10	-	-	HYPH
cord-014597-66vd2mdu	2002	11	stars	star	NNS
cord-014597-66vd2mdu	2002	12	,	,	,
cord-014597-66vd2mdu	2002	13	which	which	WDT
cord-014597-66vd2mdu	2002	14	can	can	MD
cord-014597-66vd2mdu	2002	15	be	be	VB
cord-014597-66vd2mdu	2002	16	retained	retain	VBN
cord-014597-66vd2mdu	2002	17	/	/	SYM
cord-014597-66vd2mdu	2002	18	directed	direct	VBN
cord-014597-66vd2mdu	2002	19	by	by	IN
cord-014597-66vd2mdu	2002	20	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2002	21	fields	field	NNS
cord-014597-66vd2mdu	2002	22	,	,	,
cord-014597-66vd2mdu	2002	23	could	could	MD
cord-014597-66vd2mdu	2002	24	become	become	VB
cord-014597-66vd2mdu	2002	25	options	option	NNS
cord-014597-66vd2mdu	2002	26	for	for	IN
cord-014597-66vd2mdu	2002	27	non	non	JJ
cord-014597-66vd2mdu	2002	28	-	-	JJ
cord-014597-66vd2mdu	2002	29	viral	viral	JJ
cord-014597-66vd2mdu	2002	30	gene	gene	NN
cord-014597-66vd2mdu	2002	31	delivery	delivery	NN
cord-014597-66vd2mdu	2002	32	in	in	IN
cord-014597-66vd2mdu	2002	33	vivo	vivo	NNP
cord-014597-66vd2mdu	2002	34	.	.	.
cord-014597-66vd2mdu	2003	1	The	the	DT
cord-014597-66vd2mdu	2003	2	increasing	increase	VBG
cord-014597-66vd2mdu	2003	3	demand	demand	NN
cord-014597-66vd2mdu	2003	4	for	for	IN
cord-014597-66vd2mdu	2003	5	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	2003	6	antibodies	antibody	NNS
cord-014597-66vd2mdu	2003	7	has	have	VBZ
cord-014597-66vd2mdu	2003	8	necessitated	necessitate	VBN
cord-014597-66vd2mdu	2003	9	the	the	DT
cord-014597-66vd2mdu	2003	10	need	need	NN
cord-014597-66vd2mdu	2003	11	to	to	TO
cord-014597-66vd2mdu	2003	12	increase	increase	VB
cord-014597-66vd2mdu	2003	13	the	the	DT
cord-014597-66vd2mdu	2003	14	productivity	productivity	NN
cord-014597-66vd2mdu	2003	15	of	of	IN
cord-014597-66vd2mdu	2003	16	current	current	JJ
cord-014597-66vd2mdu	2003	17	industrial	industrial	JJ
cord-014597-66vd2mdu	2003	18	cell	cell	NN
cord-014597-66vd2mdu	2003	19	lines	line	NNS
cord-014597-66vd2mdu	2003	20	.	.	.
cord-014597-66vd2mdu	2004	1	In	in	IN
cord-014597-66vd2mdu	2004	2	our	-PRON-	PRP$
cord-014597-66vd2mdu	2004	3	earlier	early	JJR
cord-014597-66vd2mdu	2004	4	study	study	NN
cord-014597-66vd2mdu	2004	5	[	[	-LRB-
cord-014597-66vd2mdu	2004	6	1	1	CD
cord-014597-66vd2mdu	2004	7	]	]	-RRB-
cord-014597-66vd2mdu	2004	8	,	,	,
cord-014597-66vd2mdu	2004	9	we	-PRON-	PRP
cord-014597-66vd2mdu	2004	10	had	have	VBD
cord-014597-66vd2mdu	2004	11	shown	show	VBN
cord-014597-66vd2mdu	2004	12	that	that	DT
cord-014597-66vd2mdu	2004	13	treatment	treatment	NN
cord-014597-66vd2mdu	2004	14	with	with	IN
cord-014597-66vd2mdu	2004	15	ER	er	NN
cord-014597-66vd2mdu	2004	16	-	-	HYPH
cord-014597-66vd2mdu	2004	17	stress	stress	NN
cord-014597-66vd2mdu	2004	18	inducer	inducer	NN
cord-014597-66vd2mdu	2004	19	,	,	,
cord-014597-66vd2mdu	2004	20	Tunicamycin	Tunicamycin	NNP
cord-014597-66vd2mdu	2004	21	significantly	significantly	RB
cord-014597-66vd2mdu	2004	22	increased	increase	VBD
cord-014597-66vd2mdu	2004	23	the	the	DT
cord-014597-66vd2mdu	2004	24	titers	titer	NNS
cord-014597-66vd2mdu	2004	25	and	and	CC
cord-014597-66vd2mdu	2004	26	productivity	productivity	NN
cord-014597-66vd2mdu	2004	27	in	in	IN
cord-014597-66vd2mdu	2004	28	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2004	29	CHO	CHO	NNP
cord-014597-66vd2mdu	2004	30	cell	cell	NN
cord-014597-66vd2mdu	2004	31	lines	line	NNS
cord-014597-66vd2mdu	2004	32	with	with	IN
cord-014597-66vd2mdu	2004	33	a	a	DT
cord-014597-66vd2mdu	2004	34	simultaneous	simultaneous	JJ
cord-014597-66vd2mdu	2004	35	upregulation	upregulation	NN
cord-014597-66vd2mdu	2004	36	of	of	IN
cord-014597-66vd2mdu	2004	37	many	many	JJ
cord-014597-66vd2mdu	2004	38	genes	gene	NNS
cord-014597-66vd2mdu	2004	39	from	from	IN
cord-014597-66vd2mdu	2004	40	the	the	DT
cord-014597-66vd2mdu	2004	41	unfolded	unfolded	JJ
cord-014597-66vd2mdu	2004	42	protein	protein	NN
cord-014597-66vd2mdu	2004	43	response	response	NN
cord-014597-66vd2mdu	2004	44	pathway	pathway	NN
cord-014597-66vd2mdu	2004	45	(	(	-LRB-
cord-014597-66vd2mdu	2004	46	UPR	UPR	NNP
cord-014597-66vd2mdu	2004	47	)	)	-RRB-
cord-014597-66vd2mdu	2004	48	.	.	.
cord-014597-66vd2mdu	2005	1	However	however	RB
cord-014597-66vd2mdu	2005	2	the	the	DT
cord-014597-66vd2mdu	2005	3	loss	loss	NN
cord-014597-66vd2mdu	2005	4	in	in	IN
cord-014597-66vd2mdu	2005	5	cell	cell	NN
cord-014597-66vd2mdu	2005	6	viability	viability	NN
cord-014597-66vd2mdu	2005	7	prevented	prevent	VBD
cord-014597-66vd2mdu	2005	8	a	a	DT
cord-014597-66vd2mdu	2005	9	sustained	sustained	JJ
cord-014597-66vd2mdu	2005	10	increase	increase	NN
cord-014597-66vd2mdu	2005	11	in	in	IN
cord-014597-66vd2mdu	2005	12	titers	titer	NNS
cord-014597-66vd2mdu	2005	13	.	.	.
cord-014597-66vd2mdu	2006	1	In	in	IN
cord-014597-66vd2mdu	2006	2	the	the	DT
cord-014597-66vd2mdu	2006	3	current	current	JJ
cord-014597-66vd2mdu	2006	4	study	study	NN
cord-014597-66vd2mdu	2006	5	we	-PRON-	PRP
cord-014597-66vd2mdu	2006	6	explore	explore	VBP
cord-014597-66vd2mdu	2006	7	the	the	DT
cord-014597-66vd2mdu	2006	8	effect	effect	NN
cord-014597-66vd2mdu	2006	9	of	of	IN
cord-014597-66vd2mdu	2006	10	varying	vary	VBG
cord-014597-66vd2mdu	2006	11	concentrations	concentration	NNS
cord-014597-66vd2mdu	2006	12	of	of	IN
cord-014597-66vd2mdu	2006	13	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2006	14	and	and	CC
cord-014597-66vd2mdu	2006	15	treatment	treatment	NN
cord-014597-66vd2mdu	2006	16	times	time	NNS
cord-014597-66vd2mdu	2006	17	,	,	,
cord-014597-66vd2mdu	2006	18	such	such	JJ
cord-014597-66vd2mdu	2006	19	as	as	IN
cord-014597-66vd2mdu	2006	20	to	to	TO
cord-014597-66vd2mdu	2006	21	modulate	modulate	VB
cord-014597-66vd2mdu	2006	22	the	the	DT
cord-014597-66vd2mdu	2006	23	increase	increase	NN
cord-014597-66vd2mdu	2006	24	in	in	IN
cord-014597-66vd2mdu	2006	25	protein	protein	NN
cord-014597-66vd2mdu	2006	26	folding	folding	NN
cord-014597-66vd2mdu	2006	27	capacity	capacity	NN
cord-014597-66vd2mdu	2006	28	while	while	IN
cord-014597-66vd2mdu	2006	29	preventing	prevent	VBG
cord-014597-66vd2mdu	2006	30	induction	induction	NN
cord-014597-66vd2mdu	2006	31	of	of	IN
cord-014597-66vd2mdu	2006	32	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	2006	33	.	.	.
cord-014597-66vd2mdu	2007	1	Anti	anti	JJ
cord-014597-66vd2mdu	2007	2	-	-	JJ
cord-014597-66vd2mdu	2007	3	rhesus	rhesus	JJ
cord-014597-66vd2mdu	2007	4	IgG	igg	NN
cord-014597-66vd2mdu	2007	5	-	-	HYPH
cord-014597-66vd2mdu	2007	6	secreting	secrete	VBG
cord-014597-66vd2mdu	2007	7	CHO	CHO	NNP
cord-014597-66vd2mdu	2007	8	cells	cell	NNS
cord-014597-66vd2mdu	2007	9	[	[	-LRB-
cord-014597-66vd2mdu	2007	10	2	2	CD
cord-014597-66vd2mdu	2007	11	]	]	-RRB-
cord-014597-66vd2mdu	2007	12	were	be	VBD
cord-014597-66vd2mdu	2007	13	cultured	culture	VBN
cord-014597-66vd2mdu	2007	14	in	in	IN
cord-014597-66vd2mdu	2007	15	SF	SF	NNP
cord-014597-66vd2mdu	2007	16	-	-	HYPH
cord-014597-66vd2mdu	2007	17	CDM	CDM	NNP
cord-014597-66vd2mdu	2007	18	in	in	IN
cord-014597-66vd2mdu	2007	19	125	125	CD
cord-014597-66vd2mdu	2007	20	mL	ml	NN
cord-014597-66vd2mdu	2007	21	shake	shake	NN
cord-014597-66vd2mdu	2007	22	flasks	flask	NNS
cord-014597-66vd2mdu	2007	23	.	.	.
cord-014597-66vd2mdu	2008	1	The	the	DT
cord-014597-66vd2mdu	2008	2	cells	cell	NNS
cord-014597-66vd2mdu	2008	3	were	be	VBD
cord-014597-66vd2mdu	2008	4	treated	treat	VBN
cord-014597-66vd2mdu	2008	5	with	with	IN
cord-014597-66vd2mdu	2008	6	varying	vary	VBG
cord-014597-66vd2mdu	2008	7	concentrations	concentration	NNS
cord-014597-66vd2mdu	2008	8	(	(	-LRB-
cord-014597-66vd2mdu	2008	9	30	30	CD
cord-014597-66vd2mdu	2008	10	-	-	SYM
cord-014597-66vd2mdu	2008	11	500	500	CD
cord-014597-66vd2mdu	2008	12	ng	ng	NN
cord-014597-66vd2mdu	2008	13	/	/	SYM
cord-014597-66vd2mdu	2008	14	mL	ml	NN
cord-014597-66vd2mdu	2008	15	)	)	-RRB-
cord-014597-66vd2mdu	2008	16	of	of	IN
cord-014597-66vd2mdu	2008	17	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2008	18	in	in	IN
cord-014597-66vd2mdu	2008	19	a	a	DT
cord-014597-66vd2mdu	2008	20	batch	batch	NN
cord-014597-66vd2mdu	2008	21	culture	culture	NN
cord-014597-66vd2mdu	2008	22	.	.	.
cord-014597-66vd2mdu	2009	1	Further	further	RB
cord-014597-66vd2mdu	2009	2	,	,	,
cord-014597-66vd2mdu	2009	3	the	the	DT
cord-014597-66vd2mdu	2009	4	effect	effect	NN
cord-014597-66vd2mdu	2009	5	of	of	IN
cord-014597-66vd2mdu	2009	6	treatment	treatment	NN
cord-014597-66vd2mdu	2009	7	with	with	IN
cord-014597-66vd2mdu	2009	8	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2009	9	for	for	IN
cord-014597-66vd2mdu	2009	10	short	short	JJ
cord-014597-66vd2mdu	2009	11	periods	period	NNS
cord-014597-66vd2mdu	2009	12	of	of	IN
cord-014597-66vd2mdu	2009	13	time	time	NN
cord-014597-66vd2mdu	2009	14	(	(	-LRB-
cord-014597-66vd2mdu	2009	15	24	24	CD
cord-014597-66vd2mdu	2009	16	hrs	hrs	NN
cord-014597-66vd2mdu	2009	17	)	)	-RRB-
cord-014597-66vd2mdu	2009	18	was	be	VBD
cord-014597-66vd2mdu	2009	19	also	also	RB
cord-014597-66vd2mdu	2009	20	evaluated	evaluate	VBN
cord-014597-66vd2mdu	2009	21	.	.	.
cord-014597-66vd2mdu	2010	1	IgG	IgG	NNP
cord-014597-66vd2mdu	2010	2	titers	titers	NNP
cord-014597-66vd2mdu	2010	3	and	and	CC
cord-014597-66vd2mdu	2010	4	mRNA	mRNA	NNP
cord-014597-66vd2mdu	2010	5	expression	expression	NN
cord-014597-66vd2mdu	2010	6	levels	level	NNS
cord-014597-66vd2mdu	2010	7	were	be	VBD
cord-014597-66vd2mdu	2010	8	quantified	quantify	VBN
cord-014597-66vd2mdu	2010	9	using	use	VBG
cord-014597-66vd2mdu	2010	10	ELISA	ELISA	NNP
cord-014597-66vd2mdu	2010	11	and	and	CC
cord-014597-66vd2mdu	2010	12	qRT	qRT	NNP
cord-014597-66vd2mdu	2010	13	-	-	HYPH
cord-014597-66vd2mdu	2010	14	PCR	PCR	NNP
cord-014597-66vd2mdu	2010	15	(	(	-LRB-
cord-014597-66vd2mdu	2010	16	Illumina	Illumina	NNP
cord-014597-66vd2mdu	2010	17	)	)	-RRB-
cord-014597-66vd2mdu	2010	18	,	,	,
cord-014597-66vd2mdu	2010	19	respectively	respectively	RB
cord-014597-66vd2mdu	2010	20	.	.	.
cord-014597-66vd2mdu	2011	1	Results	result	NNS
cord-014597-66vd2mdu	2011	2	CHO	CHO	NNP
cord-014597-66vd2mdu	2011	3	cells	cell	NNS
cord-014597-66vd2mdu	2011	4	were	be	VBD
cord-014597-66vd2mdu	2011	5	treated	treat	VBN
cord-014597-66vd2mdu	2011	6	with	with	IN
cord-014597-66vd2mdu	2011	7	different	different	JJ
cord-014597-66vd2mdu	2011	8	concentrations	concentration	NNS
cord-014597-66vd2mdu	2011	9	of	of	IN
cord-014597-66vd2mdu	2011	10	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2011	11	and	and	CC
cord-014597-66vd2mdu	2011	12	cultured	culture	VBN
cord-014597-66vd2mdu	2011	13	in	in	IN
cord-014597-66vd2mdu	2011	14	a	a	DT
cord-014597-66vd2mdu	2011	15	batch	batch	NN
cord-014597-66vd2mdu	2011	16	for	for	IN
cord-014597-66vd2mdu	2011	17	8	8	CD
cord-014597-66vd2mdu	2011	18	days	day	NNS
cord-014597-66vd2mdu	2011	19	(	(	-LRB-
cord-014597-66vd2mdu	2011	20	referred	refer	VBN
cord-014597-66vd2mdu	2011	21	as	as	IN
cord-014597-66vd2mdu	2011	22	Continuous	continuous	JJ
cord-014597-66vd2mdu	2011	23	treatment	treatment	NN
cord-014597-66vd2mdu	2011	24	/	/	SYM
cord-014597-66vd2mdu	2011	25	CTE	CTE	NNP
cord-014597-66vd2mdu	2011	26	)	)	-RRB-
cord-014597-66vd2mdu	2011	27	.	.	.
cord-014597-66vd2mdu	2012	1	Figure	figure	NN
cord-014597-66vd2mdu	2012	2	1a	1a	CD
cord-014597-66vd2mdu	2012	3	presents	present	VBZ
cord-014597-66vd2mdu	2012	4	the	the	DT
cord-014597-66vd2mdu	2012	5	maximum	maximum	JJ
cord-014597-66vd2mdu	2012	6	VCD	vcd	NN
cord-014597-66vd2mdu	2012	7	and	and	CC
cord-014597-66vd2mdu	2012	8	%	%	NN
cord-014597-66vd2mdu	2012	9	drop	drop	NN
cord-014597-66vd2mdu	2012	10	in	in	IN
cord-014597-66vd2mdu	2012	11	viability	viability	NN
cord-014597-66vd2mdu	2012	12	under	under	IN
cord-014597-66vd2mdu	2012	13	treatment	treatment	NN
cord-014597-66vd2mdu	2012	14	.	.	.
cord-014597-66vd2mdu	2013	1	A	a	DT
cord-014597-66vd2mdu	2013	2	dose	dose	NN
cord-014597-66vd2mdu	2013	3	-	-	HYPH
cord-014597-66vd2mdu	2013	4	dependent	dependent	JJ
cord-014597-66vd2mdu	2013	5	inhibitory	inhibitory	JJ
cord-014597-66vd2mdu	2013	6	effect	effect	NN
cord-014597-66vd2mdu	2013	7	is	be	VBZ
cord-014597-66vd2mdu	2013	8	observed	observe	VBN
cord-014597-66vd2mdu	2013	9	on	on	IN
cord-014597-66vd2mdu	2013	10	growth	growth	NN
cord-014597-66vd2mdu	2013	11	and	and	CC
cord-014597-66vd2mdu	2013	12	viability	viability	NN
cord-014597-66vd2mdu	2013	13	of	of	IN
cord-014597-66vd2mdu	2013	14	cells	cell	NNS
cord-014597-66vd2mdu	2013	15	in	in	IN
cord-014597-66vd2mdu	2013	16	CTE	CTE	NNP
cord-014597-66vd2mdu	2013	17	-	-	HYPH
cord-014597-66vd2mdu	2013	18	cultures	culture	NNS
cord-014597-66vd2mdu	2013	19	,	,	,
cord-014597-66vd2mdu	2013	20	with	with	IN
cord-014597-66vd2mdu	2013	21	minimal	minimal	JJ
cord-014597-66vd2mdu	2013	22	inhibition	inhibition	NN
cord-014597-66vd2mdu	2013	23	as	as	IN
cord-014597-66vd2mdu	2013	24	lower	low	JJR
cord-014597-66vd2mdu	2013	25	concentrations	concentration	NNS
cord-014597-66vd2mdu	2013	26	.	.	.
cord-014597-66vd2mdu	2014	1	Contrastingly	contrastingly	RB
cord-014597-66vd2mdu	2014	2	,	,	,
cord-014597-66vd2mdu	2014	3	IgG	igg	NN
cord-014597-66vd2mdu	2014	4	titers	titer	NNS
cord-014597-66vd2mdu	2014	5	(	(	-LRB-
cord-014597-66vd2mdu	2014	6	Fig	Fig	NNP
cord-014597-66vd2mdu	2014	7	.	.	.
cord-014597-66vd2mdu	2014	8	1b	1b	LS
cord-014597-66vd2mdu	2014	9	)	)	-RRB-
cord-014597-66vd2mdu	2014	10	were	be	VBD
cord-014597-66vd2mdu	2014	11	higher	high	JJR
cord-014597-66vd2mdu	2014	12	in	in	IN
cord-014597-66vd2mdu	2014	13	treated	treat	VBN
cord-014597-66vd2mdu	2014	14	cultures	culture	NNS
cord-014597-66vd2mdu	2014	15	w.r.t	w.r.t	JJ
cord-014597-66vd2mdu	2014	16	.	.	.
cord-014597-66vd2mdu	2014	17	control	control	NN
cord-014597-66vd2mdu	2014	18	in	in	IN
cord-014597-66vd2mdu	2014	19	initial	initial	JJ
cord-014597-66vd2mdu	2014	20	phase	phase	NN
cord-014597-66vd2mdu	2014	21	of	of	IN
cord-014597-66vd2mdu	2014	22	the	the	DT
cord-014597-66vd2mdu	2014	23	cultures	culture	NNS
cord-014597-66vd2mdu	2014	24	at	at	IN
cord-014597-66vd2mdu	2014	25	all	all	PDT
cord-014597-66vd2mdu	2014	26	the	the	DT
cord-014597-66vd2mdu	2014	27	concentrations	concentration	NNS
cord-014597-66vd2mdu	2014	28	of	of	IN
cord-014597-66vd2mdu	2014	29	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2014	30	.	.	.
cord-014597-66vd2mdu	2015	1	The	the	DT
cord-014597-66vd2mdu	2015	2	per	per	IN
cord-014597-66vd2mdu	2015	3	-	-	HYPH
cord-014597-66vd2mdu	2015	4	cell	cell	NN
cord-014597-66vd2mdu	2015	5	productivity	productivity	NN
cord-014597-66vd2mdu	2015	6	(	(	-LRB-
cord-014597-66vd2mdu	2015	7	Fig	Fig	NNP
cord-014597-66vd2mdu	2015	8	.	.	.
cord-014597-66vd2mdu	2015	9	1c	1c	NNP
cord-014597-66vd2mdu	2015	10	)	)	-RRB-
cord-014597-66vd2mdu	2015	11	also	also	RB
cord-014597-66vd2mdu	2015	12	showed	show	VBD
cord-014597-66vd2mdu	2015	13	a	a	DT
cord-014597-66vd2mdu	2015	14	significant	significant	JJ
cord-014597-66vd2mdu	2015	15	increase	increase	NN
cord-014597-66vd2mdu	2015	16	w.r.t	w.r.t	JJ
cord-014597-66vd2mdu	2015	17	control	control	NN
cord-014597-66vd2mdu	2015	18	at	at	IN
cord-014597-66vd2mdu	2015	19	all	all	PDT
cord-014597-66vd2mdu	2015	20	the	the	DT
cord-014597-66vd2mdu	2015	21	concentrations	concentration	NNS
cord-014597-66vd2mdu	2015	22	of	of	IN
cord-014597-66vd2mdu	2015	23	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2015	24	.	.	.
cord-014597-66vd2mdu	2016	1	However	however	RB
cord-014597-66vd2mdu	2016	2	,	,	,
cord-014597-66vd2mdu	2016	3	the	the	DT
cord-014597-66vd2mdu	2016	4	increased	increase	VBN
cord-014597-66vd2mdu	2016	5	productivity	productivity	NN
cord-014597-66vd2mdu	2016	6	due	due	IN
cord-014597-66vd2mdu	2016	7	to	to	IN
cord-014597-66vd2mdu	2016	8	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2016	9	was	be	VBD
cord-014597-66vd2mdu	2016	10	not	not	RB
cord-014597-66vd2mdu	2016	11	sustained	sustain	VBN
cord-014597-66vd2mdu	2016	12	and	and	CC
cord-014597-66vd2mdu	2016	13	levels	level	NNS
cord-014597-66vd2mdu	2016	14	become	become	VBP
cord-014597-66vd2mdu	2016	15	similar	similar	JJ
cord-014597-66vd2mdu	2016	16	to	to	TO
cord-014597-66vd2mdu	2016	17	control	control	NN
cord-014597-66vd2mdu	2016	18	after	after	IN
cord-014597-66vd2mdu	2016	19	day	day	NN
cord-014597-66vd2mdu	2016	20	3	3	CD
cord-014597-66vd2mdu	2016	21	(	(	-LRB-
cord-014597-66vd2mdu	2016	22	data	datum	NNS
cord-014597-66vd2mdu	2016	23	not	not	RB
cord-014597-66vd2mdu	2016	24	shown	show	VBN
cord-014597-66vd2mdu	2016	25	)	)	-RRB-
cord-014597-66vd2mdu	2016	26	.	.	.
cord-014597-66vd2mdu	2017	1	To	to	TO
cord-014597-66vd2mdu	2017	2	prevent	prevent	VB
cord-014597-66vd2mdu	2017	3	loss	loss	NN
cord-014597-66vd2mdu	2017	4	of	of	IN
cord-014597-66vd2mdu	2017	5	viability	viability	NN
cord-014597-66vd2mdu	2017	6	due	due	IN
cord-014597-66vd2mdu	2017	7	to	to	IN
cord-014597-66vd2mdu	2017	8	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2017	9	,	,	,
cord-014597-66vd2mdu	2017	10	the	the	DT
cord-014597-66vd2mdu	2017	11	effect	effect	NN
cord-014597-66vd2mdu	2017	12	of	of	IN
cord-014597-66vd2mdu	2017	13	short	short	JJ
cord-014597-66vd2mdu	2017	14	-	-	HYPH
cord-014597-66vd2mdu	2017	15	term	term	NN
cord-014597-66vd2mdu	2017	16	treatment	treatment	NN
cord-014597-66vd2mdu	2017	17	(	(	-LRB-
cord-014597-66vd2mdu	2017	18	STE	STE	NNP
cord-014597-66vd2mdu	2017	19	)	)	-RRB-
cord-014597-66vd2mdu	2017	20	with	with	IN
cord-014597-66vd2mdu	2017	21	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2017	22	was	be	VBD
cord-014597-66vd2mdu	2017	23	explored	explore	VBN
cord-014597-66vd2mdu	2017	24	.	.	.
cord-014597-66vd2mdu	2018	1	Cells	cell	NNS
cord-014597-66vd2mdu	2018	2	treated	treat	VBN
cord-014597-66vd2mdu	2018	3	with	with	IN
cord-014597-66vd2mdu	2018	4	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2018	5	for	for	IN
cord-014597-66vd2mdu	2018	6	24	24	CD
cord-014597-66vd2mdu	2018	7	hours	hour	NNS
cord-014597-66vd2mdu	2018	8	were	be	VBD
cord-014597-66vd2mdu	2018	9	harvested	harvest	VBN
cord-014597-66vd2mdu	2018	10	(	(	-LRB-
cord-014597-66vd2mdu	2018	11	corresponding	correspond	VBG
cord-014597-66vd2mdu	2018	12	to	to	IN
cord-014597-66vd2mdu	2018	13	day	day	NN
cord-014597-66vd2mdu	2018	14	2	2	CD
cord-014597-66vd2mdu	2018	15	of	of	IN
cord-014597-66vd2mdu	2018	16	CTE	CTE	NNP
cord-014597-66vd2mdu	2018	17	cultures	culture	NNS
cord-014597-66vd2mdu	2018	18	)	)	-RRB-
cord-014597-66vd2mdu	2018	19	and	and	CC
cord-014597-66vd2mdu	2018	20	inoculated	inoculate	VBN
cord-014597-66vd2mdu	2018	21	in	in	IN
cord-014597-66vd2mdu	2018	22	fresh	fresh	JJ
cord-014597-66vd2mdu	2018	23	media	medium	NNS
cord-014597-66vd2mdu	2018	24	.	.	.
cord-014597-66vd2mdu	2019	1	The	the	DT
cord-014597-66vd2mdu	2019	2	STE	STE	NNP
cord-014597-66vd2mdu	2019	3	-	-	HYPH
cord-014597-66vd2mdu	2019	4	cultures	culture	NNS
cord-014597-66vd2mdu	2019	5	showed	show	VBD
cord-014597-66vd2mdu	2019	6	improved	improved	JJ
cord-014597-66vd2mdu	2019	7	viability	viability	NN
cord-014597-66vd2mdu	2019	8	and	and	CC
cord-014597-66vd2mdu	2019	9	higher	high	JJR
cord-014597-66vd2mdu	2019	10	maximum	maximum	JJ
cord-014597-66vd2mdu	2019	11	VCD	vcd	NN
cord-014597-66vd2mdu	2019	12	as	as	IN
cord-014597-66vd2mdu	2019	13	compared	compare	VBN
cord-014597-66vd2mdu	2019	14	to	to	IN
cord-014597-66vd2mdu	2019	15	CTE	CTE	NNP
cord-014597-66vd2mdu	2019	16	-	-	HYPH
cord-014597-66vd2mdu	2019	17	cultures	culture	NNS
cord-014597-66vd2mdu	2019	18	(	(	-LRB-
cord-014597-66vd2mdu	2019	19	Fig	Fig	NNP
cord-014597-66vd2mdu	2019	20	.	.	.
cord-014597-66vd2mdu	2019	21	1a	1a	CD
cord-014597-66vd2mdu	2019	22	)	)	-RRB-
cord-014597-66vd2mdu	2019	23	.	.	.
cord-014597-66vd2mdu	2020	1	The	the	DT
cord-014597-66vd2mdu	2020	2	fold	fold	JJ
cord-014597-66vd2mdu	2020	3	increase	increase	NN
cord-014597-66vd2mdu	2020	4	in	in	IN
cord-014597-66vd2mdu	2020	5	IgG	igg	NN
cord-014597-66vd2mdu	2020	6	titers	titer	NNS
cord-014597-66vd2mdu	2020	7	was	be	VBD
cord-014597-66vd2mdu	2020	8	not	not	RB
cord-014597-66vd2mdu	2020	9	sustained	sustain	VBN
cord-014597-66vd2mdu	2020	10	beyond	beyond	IN
cord-014597-66vd2mdu	2020	11	day	day	NN
cord-014597-66vd2mdu	2020	12	1	1	CD
cord-014597-66vd2mdu	2020	13	-	-	SYM
cord-014597-66vd2mdu	2020	14	2	2	CD
cord-014597-66vd2mdu	2020	15	in	in	IN
cord-014597-66vd2mdu	2020	16	STEcultures	steculture	NNS
cord-014597-66vd2mdu	2021	1	(	(	-LRB-
cord-014597-66vd2mdu	2021	2	Fig	Fig	NNP
cord-014597-66vd2mdu	2021	3	.	.	.
cord-014597-66vd2mdu	2021	4	1d	1d	CD
cord-014597-66vd2mdu	2021	5	)	)	-RRB-
cord-014597-66vd2mdu	2022	1	but	but	CC
cord-014597-66vd2mdu	2022	2	significant	significant	JJ
cord-014597-66vd2mdu	2022	3	increase	increase	NN
cord-014597-66vd2mdu	2022	4	in	in	IN
cord-014597-66vd2mdu	2022	5	productivity	productivity	NN
cord-014597-66vd2mdu	2022	6	was	be	VBD
cord-014597-66vd2mdu	2022	7	seen	see	VBN
cord-014597-66vd2mdu	2022	8	in	in	IN
cord-014597-66vd2mdu	2022	9	the	the	DT
cord-014597-66vd2mdu	2022	10	initial	initial	JJ
cord-014597-66vd2mdu	2022	11	phase	phase	NN
cord-014597-66vd2mdu	2022	12	(	(	-LRB-
cord-014597-66vd2mdu	2022	13	Fig	Fig	NNP
cord-014597-66vd2mdu	2022	14	.	.	.
cord-014597-66vd2mdu	2022	15	1e	1e	LS
cord-014597-66vd2mdu	2022	16	)	)	-RRB-
cord-014597-66vd2mdu	2022	17	.	.	.
cord-014597-66vd2mdu	2023	1	Further	further	RB
cord-014597-66vd2mdu	2023	2	,	,	,
cord-014597-66vd2mdu	2023	3	the	the	DT
cord-014597-66vd2mdu	2023	4	cells	cell	NNS
cord-014597-66vd2mdu	2023	5	were	be	VBD
cord-014597-66vd2mdu	2023	6	adapted	adapt	VBN
cord-014597-66vd2mdu	2023	7	over	over	IN
cord-014597-66vd2mdu	2023	8	25	25	CD
cord-014597-66vd2mdu	2023	9	continuous	continuous	JJ
cord-014597-66vd2mdu	2023	10	generations	generation	NNS
cord-014597-66vd2mdu	2023	11	under	under	IN
cord-014597-66vd2mdu	2023	12	30ng	30ng	NN
cord-014597-66vd2mdu	2023	13	/	/	SYM
cord-014597-66vd2mdu	2023	14	mL	ml	NN
cord-014597-66vd2mdu	2023	15	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2023	16	.	.	.
cord-014597-66vd2mdu	2024	1	The	the	DT
cord-014597-66vd2mdu	2024	2	adapted	adapt	VBN
cord-014597-66vd2mdu	2024	3	cells	cell	NNS
cord-014597-66vd2mdu	2024	4	had	have	VBD
cord-014597-66vd2mdu	2024	5	overall	overall	RB
cord-014597-66vd2mdu	2024	6	1.3-fold	1.3-fold	CD
cord-014597-66vd2mdu	2024	7	higher	high	JJR
cord-014597-66vd2mdu	2024	8	productivity	productivity	NN
cord-014597-66vd2mdu	2024	9	,	,	,
cord-014597-66vd2mdu	2024	10	as	as	IN
cord-014597-66vd2mdu	2024	11	compared	compare	VBN
cord-014597-66vd2mdu	2024	12	to	to	TO
cord-014597-66vd2mdu	2024	13	control	control	VB
cord-014597-66vd2mdu	2024	14	(	(	-LRB-
cord-014597-66vd2mdu	2024	15	Fig	Fig	NNP
cord-014597-66vd2mdu	2024	16	.	.	.
cord-014597-66vd2mdu	2024	17	1f	1f	CD
cord-014597-66vd2mdu	2024	18	)	)	-RRB-
cord-014597-66vd2mdu	2024	19	,	,	,
cord-014597-66vd2mdu	2024	20	in	in	IN
cord-014597-66vd2mdu	2024	21	a	a	DT
cord-014597-66vd2mdu	2024	22	batch	batch	NN
cord-014597-66vd2mdu	2024	23	culture	culture	NN
cord-014597-66vd2mdu	2024	24	.	.	.
cord-014597-66vd2mdu	2025	1	To	to	TO
cord-014597-66vd2mdu	2025	2	understand	understand	VB
cord-014597-66vd2mdu	2025	3	the	the	DT
cord-014597-66vd2mdu	2025	4	molecular	molecular	JJ
cord-014597-66vd2mdu	2025	5	basis	basis	NN
cord-014597-66vd2mdu	2025	6	of	of	IN
cord-014597-66vd2mdu	2025	7	increase	increase	NN
cord-014597-66vd2mdu	2025	8	in	in	IN
cord-014597-66vd2mdu	2025	9	productivity	productivity	NN
cord-014597-66vd2mdu	2025	10	,	,	,
cord-014597-66vd2mdu	2025	11	mRNA	mrna	NN
cord-014597-66vd2mdu	2025	12	expression	expression	NN
cord-014597-66vd2mdu	2025	13	level	level	NN
cord-014597-66vd2mdu	2025	14	of	of	IN
cord-014597-66vd2mdu	2025	15	key	key	JJ
cord-014597-66vd2mdu	2025	16	genes	gene	NNS
cord-014597-66vd2mdu	2025	17	was	be	VBD
cord-014597-66vd2mdu	2025	18	determined	determine	VBN
cord-014597-66vd2mdu	2025	19	.	.	.
cord-014597-66vd2mdu	2026	1	XBP1s	XBP1s	NNP
cord-014597-66vd2mdu	2026	2	is	be	VBZ
cord-014597-66vd2mdu	2026	3	a	a	DT
cord-014597-66vd2mdu	2026	4	transcription	transcription	NN
cord-014597-66vd2mdu	2026	5	factor	factor	NN
cord-014597-66vd2mdu	2026	6	involved	involve	VBN
cord-014597-66vd2mdu	2026	7	in	in	IN
cord-014597-66vd2mdu	2026	8	activation	activation	NN
cord-014597-66vd2mdu	2026	9	of	of	IN
cord-014597-66vd2mdu	2026	10	chaperones	chaperone	NNS
cord-014597-66vd2mdu	2026	11	(	(	-LRB-
cord-014597-66vd2mdu	2026	12	like	like	IN
cord-014597-66vd2mdu	2026	13	Grp78	Grp78	NNP
cord-014597-66vd2mdu	2026	14	,	,	,
cord-014597-66vd2mdu	2026	15	Calreticulin	Calreticulin	NNP
cord-014597-66vd2mdu	2026	16	)	)	-RRB-
cord-014597-66vd2mdu	2026	17	and	and	CC
cord-014597-66vd2mdu	2026	18	apoptotic	apoptotic	JJ
cord-014597-66vd2mdu	2026	19	genes	gene	NNS
cord-014597-66vd2mdu	2026	20	(	(	-LRB-
cord-014597-66vd2mdu	2026	21	such	such	JJ
cord-014597-66vd2mdu	2026	22	as	as	IN
cord-014597-66vd2mdu	2026	23	CHOP	CHOP	NNP
cord-014597-66vd2mdu	2026	24	)	)	-RRB-
cord-014597-66vd2mdu	2026	25	.	.	.
cord-014597-66vd2mdu	2027	1	Significant	significant	JJ
cord-014597-66vd2mdu	2027	2	increase	increase	NN
cord-014597-66vd2mdu	2027	3	in	in	IN
cord-014597-66vd2mdu	2027	4	the	the	DT
cord-014597-66vd2mdu	2027	5	levels	level	NNS
cord-014597-66vd2mdu	2027	6	of	of	IN
cord-014597-66vd2mdu	2027	7	calreticulin	calreticulin	NNP
cord-014597-66vd2mdu	2027	8	was	be	VBD
cord-014597-66vd2mdu	2027	9	seen	see	VBN
cord-014597-66vd2mdu	2027	10	on	on	IN
cord-014597-66vd2mdu	2027	11	treatment	treatment	NN
cord-014597-66vd2mdu	2027	12	with	with	IN
cord-014597-66vd2mdu	2027	13	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2027	14	(	(	-LRB-
cord-014597-66vd2mdu	2027	15	Fig	Fig	NNP
cord-014597-66vd2mdu	2027	16	.	.	.
cord-014597-66vd2mdu	2027	17	1	1	CD
cord-014597-66vd2mdu	2027	18	g	g	NN
cord-014597-66vd2mdu	2027	19	)	)	-RRB-
cord-014597-66vd2mdu	2027	20	.	.	.
cord-014597-66vd2mdu	2028	1	Both	both	CC
cord-014597-66vd2mdu	2028	2	XBP1s	XBP1s	NNP
cord-014597-66vd2mdu	2028	3	and	and	CC
cord-014597-66vd2mdu	2029	1	Grp78	Grp78	NNP
cord-014597-66vd2mdu	2029	2	were	be	VBD
cord-014597-66vd2mdu	2029	3	marginally	marginally	RB
cord-014597-66vd2mdu	2029	4	induced	induce	VBN
cord-014597-66vd2mdu	2029	5	when	when	WRB
cord-014597-66vd2mdu	2029	6	treated	treat	VBN
cord-014597-66vd2mdu	2029	7	with	with	IN
cord-014597-66vd2mdu	2029	8	30ng	30ng	NN
cord-014597-66vd2mdu	2029	9	/	/	SYM
cord-014597-66vd2mdu	2029	10	mL	ml	NN
cord-014597-66vd2mdu	2029	11	of	of	IN
cord-014597-66vd2mdu	2029	12	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2029	13	in	in	IN
cord-014597-66vd2mdu	2029	14	both	both	DT
cord-014597-66vd2mdu	2029	15	CTE	CTE	NNP
cord-014597-66vd2mdu	2029	16	-	-	HYPH
cord-014597-66vd2mdu	2029	17	and	and	CC
cord-014597-66vd2mdu	2029	18	STE	STE	NNP
cord-014597-66vd2mdu	2029	19	-	-	HYPH
cord-014597-66vd2mdu	2029	20	cultures	culture	NNS
cord-014597-66vd2mdu	2029	21	(	(	-LRB-
cord-014597-66vd2mdu	2029	22	Fig	Fig	NNP
cord-014597-66vd2mdu	2029	23	.	.	.
cord-014597-66vd2mdu	2029	24	1h	1h	CD
cord-014597-66vd2mdu	2029	25	)	)	-RRB-
cord-014597-66vd2mdu	2029	26	,	,	,
cord-014597-66vd2mdu	2029	27	and	and	CC
cord-014597-66vd2mdu	2029	28	significantly	significantly	RB
cord-014597-66vd2mdu	2029	29	up	up	RB
cord-014597-66vd2mdu	2029	30	-	-	HYPH
cord-014597-66vd2mdu	2029	31	regulated	regulate	VBN
cord-014597-66vd2mdu	2029	32	when	when	WRB
cord-014597-66vd2mdu	2029	33	treated	treat	VBN
cord-014597-66vd2mdu	2029	34	with	with	IN
cord-014597-66vd2mdu	2029	35	500ng	500ng	NNP
cord-014597-66vd2mdu	2029	36	/	/	SYM
cord-014597-66vd2mdu	2029	37	mL	ml	NN
cord-014597-66vd2mdu	2029	38	of	of	IN
cord-014597-66vd2mdu	2029	39	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2029	40	.	.	.
cord-014597-66vd2mdu	2030	1	The	the	DT
cord-014597-66vd2mdu	2030	2	CHOP	chop	NN
cord-014597-66vd2mdu	2030	3	mRNA	mrna	NN
cord-014597-66vd2mdu	2030	4	levels	level	NNS
cord-014597-66vd2mdu	2030	5	also	also	RB
cord-014597-66vd2mdu	2030	6	increase	increase	VBP
cord-014597-66vd2mdu	2030	7	with	with	IN
cord-014597-66vd2mdu	2030	8	increasing	increase	VBG
cord-014597-66vd2mdu	2030	9	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2030	10	concentrations	concentration	NNS
cord-014597-66vd2mdu	2030	11	,	,	,
cord-014597-66vd2mdu	2030	12	with	with	IN
cord-014597-66vd2mdu	2030	13	levels	level	NNS
cord-014597-66vd2mdu	2030	14	in	in	IN
cord-014597-66vd2mdu	2030	15	STE	STE	NNP
cord-014597-66vd2mdu	2030	16	-	-	HYPH
cord-014597-66vd2mdu	2030	17	cultures	culture	NNS
cord-014597-66vd2mdu	2030	18	lower	low	JJR
cord-014597-66vd2mdu	2030	19	than	than	IN
cord-014597-66vd2mdu	2030	20	CTE	CTE	NNP
cord-014597-66vd2mdu	2030	21	-	-	HYPH
cord-014597-66vd2mdu	2030	22	cultures	culture	NNS
cord-014597-66vd2mdu	2030	23	(	(	-LRB-
cord-014597-66vd2mdu	2030	24	Fig	Fig	NNP
cord-014597-66vd2mdu	2030	25	.	.	.
cord-014597-66vd2mdu	2030	26	1h	1h	CD
cord-014597-66vd2mdu	2030	27	)	)	-RRB-
cord-014597-66vd2mdu	2030	28	.	.	.
cord-014597-66vd2mdu	2031	1	The	the	DT
cord-014597-66vd2mdu	2031	2	results	result	NNS
cord-014597-66vd2mdu	2031	3	suggest	suggest	VBP
cord-014597-66vd2mdu	2031	4	that	that	IN
cord-014597-66vd2mdu	2031	5	UPR	UPR	NNP
cord-014597-66vd2mdu	2031	6	induction	induction	NN
cord-014597-66vd2mdu	2031	7	may	may	MD
cord-014597-66vd2mdu	2031	8	be	be	VB
cord-014597-66vd2mdu	2031	9	important	important	JJ
cord-014597-66vd2mdu	2031	10	to	to	TO
cord-014597-66vd2mdu	2031	11	increase	increase	VB
cord-014597-66vd2mdu	2031	12	productivity	productivity	NN
cord-014597-66vd2mdu	2031	13	in	in	IN
cord-014597-66vd2mdu	2031	14	these	these	DT
cord-014597-66vd2mdu	2031	15	CTE	CTE	NNP
cord-014597-66vd2mdu	2031	16	/	/	SYM
cord-014597-66vd2mdu	2031	17	STE	STE	NNP
cord-014597-66vd2mdu	2031	18	-	-	HYPH
cord-014597-66vd2mdu	2031	19	cultures	culture	NNS
cord-014597-66vd2mdu	2031	20	.	.	.
cord-014597-66vd2mdu	2032	1	Note	note	VB
cord-014597-66vd2mdu	2032	2	that	that	IN
cord-014597-66vd2mdu	2032	3	,	,	,
cord-014597-66vd2mdu	2032	4	tunicamycin	tunicamycin	NNP
cord-014597-66vd2mdu	2032	5	had	have	VBD
cord-014597-66vd2mdu	2032	6	no	no	DT
cord-014597-66vd2mdu	2032	7	effect	effect	NN
cord-014597-66vd2mdu	2032	8	on	on	IN
cord-014597-66vd2mdu	2032	9	the	the	DT
cord-014597-66vd2mdu	2032	10	expression	expression	NN
cord-014597-66vd2mdu	2032	11	levels	level	NNS
cord-014597-66vd2mdu	2032	12	of	of	IN
cord-014597-66vd2mdu	2032	13	IgG	igg	NN
cord-014597-66vd2mdu	2032	14	heavy	heavy	JJ
cord-014597-66vd2mdu	2032	15	-	-	HYPH
cord-014597-66vd2mdu	2032	16	chain	chain	NN
cord-014597-66vd2mdu	2032	17	,	,	,
cord-014597-66vd2mdu	2032	18	thus	thus	RB
cord-014597-66vd2mdu	2032	19	eliminating	eliminate	VBG
cord-014597-66vd2mdu	2032	20	the	the	DT
cord-014597-66vd2mdu	2032	21	involvement	involvement	NN
cord-014597-66vd2mdu	2032	22	of	of	IN
cord-014597-66vd2mdu	2032	23	IgGHC	IgGHC	NNP
cord-014597-66vd2mdu	2032	24	-	-	HYPH
cord-014597-66vd2mdu	2032	25	mRNA	mRNA	NNP
cord-014597-66vd2mdu	2032	26	in	in	IN
cord-014597-66vd2mdu	2032	27	increasing	increase	VBG
cord-014597-66vd2mdu	2032	28	productivity	productivity	NN
cord-014597-66vd2mdu	2032	29	(	(	-LRB-
cord-014597-66vd2mdu	2032	30	Fig	Fig	NNP
cord-014597-66vd2mdu	2032	31	.	.	.
cord-014597-66vd2mdu	2032	32	1i	1i	LS
cord-014597-66vd2mdu	2032	33	)	)	-RRB-
cord-014597-66vd2mdu	2032	34	.	.	.
cord-014597-66vd2mdu	2033	1	Tunicamycin	Tunicamycin	NNP
cord-014597-66vd2mdu	2033	2	induced	induce	VBN
cord-014597-66vd2mdu	2033	3	ER	ER	NNP
cord-014597-66vd2mdu	2033	4	-	-	HYPH
cord-014597-66vd2mdu	2033	5	stress	stress	NN
cord-014597-66vd2mdu	2033	6	increased	increase	VBD
cord-014597-66vd2mdu	2033	7	productivity	productivity	NN
cord-014597-66vd2mdu	2033	8	in	in	IN
cord-014597-66vd2mdu	2033	9	the	the	DT
cord-014597-66vd2mdu	2033	10	initial	initial	JJ
cord-014597-66vd2mdu	2033	11	phase	phase	NN
cord-014597-66vd2mdu	2033	12	of	of	IN
cord-014597-66vd2mdu	2033	13	the	the	DT
cord-014597-66vd2mdu	2033	14	culture	culture	NN
cord-014597-66vd2mdu	2033	15	and	and	CC
cord-014597-66vd2mdu	2033	16	enhanced	enhance	VBN
cord-014597-66vd2mdu	2033	17	UPR	UPR	NNP
cord-014597-66vd2mdu	2033	18	-	-	HYPH
cord-014597-66vd2mdu	2033	19	mediated	mediate	VBN
cord-014597-66vd2mdu	2033	20	folding	folding	NN
cord-014597-66vd2mdu	2033	21	capacity	capacity	NN
cord-014597-66vd2mdu	2033	22	can	can	MD
cord-014597-66vd2mdu	2033	23	be	be	VB
cord-014597-66vd2mdu	2033	24	attributed	attribute	VBN
cord-014597-66vd2mdu	2033	25	as	as	IN
cord-014597-66vd2mdu	2033	26	one	one	CD
cord-014597-66vd2mdu	2033	27	of	of	IN
cord-014597-66vd2mdu	2033	28	the	the	DT
cord-014597-66vd2mdu	2033	29	reasons	reason	NNS
cord-014597-66vd2mdu	2033	30	for	for	IN
cord-014597-66vd2mdu	2033	31	it	-PRON-	PRP
cord-014597-66vd2mdu	2033	32	.	.	.
cord-014597-66vd2mdu	2034	1	At	at	IN
cord-014597-66vd2mdu	2034	2	lower	low	JJR
cord-014597-66vd2mdu	2034	3	concentrations	concentration	NNS
cord-014597-66vd2mdu	2034	4	of	of	IN
cord-014597-66vd2mdu	2034	5	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2034	6	,	,	,
cord-014597-66vd2mdu	2034	7	a	a	DT
cord-014597-66vd2mdu	2034	8	fine	fine	JJ
cord-014597-66vd2mdu	2034	9	balance	balance	NN
cord-014597-66vd2mdu	2034	10	between	between	IN
cord-014597-66vd2mdu	2034	11	optimum	optimum	JJ
cord-014597-66vd2mdu	2034	12	UPR	UPR	NNP
cord-014597-66vd2mdu	2034	13	induction	induction	NN
cord-014597-66vd2mdu	2034	14	and	and	CC
cord-014597-66vd2mdu	2034	15	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	2034	16	can	can	MD
cord-014597-66vd2mdu	2034	17	be	be	VB
cord-014597-66vd2mdu	2034	18	achieved	achieve	VBN
cord-014597-66vd2mdu	2034	19	,	,	,
cord-014597-66vd2mdu	2034	20	as	as	IN
cord-014597-66vd2mdu	2034	21	seen	see	VBN
cord-014597-66vd2mdu	2034	22	in	in	IN
cord-014597-66vd2mdu	2034	23	30ng	30ng	NN
cord-014597-66vd2mdu	2034	24	/	/	SYM
cord-014597-66vd2mdu	2034	25	mL	ml	NN
cord-014597-66vd2mdu	2034	26	tunicamycin	tunicamycin	NNS
cord-014597-66vd2mdu	2034	27	STE	STE	NNP
cord-014597-66vd2mdu	2034	28	-	-	HYPH
cord-014597-66vd2mdu	2034	29	cultures	culture	NNS
cord-014597-66vd2mdu	2034	30	.	.	.
cord-014597-66vd2mdu	2035	1	In	in	IN
cord-014597-66vd2mdu	2035	2	summary	summary	NN
cord-014597-66vd2mdu	2035	3	,	,	,
cord-014597-66vd2mdu	2035	4	this	this	DT
cord-014597-66vd2mdu	2035	5	study	study	NN
cord-014597-66vd2mdu	2035	6	demonstrates	demonstrate	VBZ
cord-014597-66vd2mdu	2035	7	an	an	DT
cord-014597-66vd2mdu	2035	8	alternate	alternate	JJ
cord-014597-66vd2mdu	2035	9	approach	approach	NN
cord-014597-66vd2mdu	2035	10	to	to	TO
cord-014597-66vd2mdu	2035	11	enhance	enhance	VB
cord-014597-66vd2mdu	2035	12	productivity	productivity	NN
cord-014597-66vd2mdu	2035	13	of	of	IN
cord-014597-66vd2mdu	2035	14	current	current	JJ
cord-014597-66vd2mdu	2035	15	industrial	industrial	JJ
cord-014597-66vd2mdu	2035	16	cell	cell	NN
cord-014597-66vd2mdu	2035	17	lines	line	NNS
cord-014597-66vd2mdu	2035	18	.	.	.
cord-014597-66vd2mdu	2036	1	Background	background	NN
cord-014597-66vd2mdu	2036	2	Chinese	chinese	JJ
cord-014597-66vd2mdu	2036	3	hamster	hamster	NN
cord-014597-66vd2mdu	2036	4	ovary	ovary	NN
cord-014597-66vd2mdu	2036	5	(	(	-LRB-
cord-014597-66vd2mdu	2036	6	CHO	CHO	NNP
cord-014597-66vd2mdu	2036	7	)	)	-RRB-
cord-014597-66vd2mdu	2036	8	cells	cell	NNS
cord-014597-66vd2mdu	2036	9	have	have	VBP
cord-014597-66vd2mdu	2036	10	been	be	VBN
cord-014597-66vd2mdu	2036	11	widely	widely	RB
cord-014597-66vd2mdu	2036	12	used	use	VBN
cord-014597-66vd2mdu	2036	13	for	for	IN
cord-014597-66vd2mdu	2036	14	the	the	DT
cord-014597-66vd2mdu	2036	15	large	large	JJ
cord-014597-66vd2mdu	2036	16	-	-	HYPH
cord-014597-66vd2mdu	2036	17	scale	scale	NN
cord-014597-66vd2mdu	2036	18	production	production	NN
cord-014597-66vd2mdu	2036	19	of	of	IN
cord-014597-66vd2mdu	2036	20	biopharmaceuticals	biopharmaceutical	NNS
cord-014597-66vd2mdu	2036	21	[	[	-LRB-
cord-014597-66vd2mdu	2036	22	1	1	CD
cord-014597-66vd2mdu	2036	23	]	]	-RRB-
cord-014597-66vd2mdu	2036	24	.	.	.
cord-014597-66vd2mdu	2037	1	To	to	TO
cord-014597-66vd2mdu	2037	2	construct	construct	VB
cord-014597-66vd2mdu	2037	3	antibody	antibody	NN
cord-014597-66vd2mdu	2037	4	-	-	HYPH
cord-014597-66vd2mdu	2037	5	producing	produce	VBG
cord-014597-66vd2mdu	2037	6	CHO	CHO	NNP
cord-014597-66vd2mdu	2037	7	cells	cell	NNS
cord-014597-66vd2mdu	2037	8	,	,	,
cord-014597-66vd2mdu	2037	9	exogenous	exogenous	JJ
cord-014597-66vd2mdu	2037	10	genes	gene	NNS
cord-014597-66vd2mdu	2037	11	encoding	encode	VBG
cord-014597-66vd2mdu	2037	12	antibodies	antibody	NNS
cord-014597-66vd2mdu	2037	13	are	be	VBP
cord-014597-66vd2mdu	2037	14	usually	usually	RB
cord-014597-66vd2mdu	2037	15	integrated	integrate	VBN
cord-014597-66vd2mdu	2037	16	into	into	IN
cord-014597-66vd2mdu	2037	17	unspecified	unspecified	JJ
cord-014597-66vd2mdu	2037	18	regions	region	NNS
cord-014597-66vd2mdu	2037	19	of	of	IN
cord-014597-66vd2mdu	2037	20	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	2037	21	(	(	-LRB-
cord-014597-66vd2mdu	2037	22	random	random	JJ
cord-014597-66vd2mdu	2037	23	integration	integration	NN
cord-014597-66vd2mdu	2037	24	)	)	-RRB-
cord-014597-66vd2mdu	2037	25	.	.	.
cord-014597-66vd2mdu	2038	1	However	however	RB
cord-014597-66vd2mdu	2038	2	,	,	,
cord-014597-66vd2mdu	2038	3	the	the	DT
cord-014597-66vd2mdu	2038	4	chromatin	chromatin	NN
cord-014597-66vd2mdu	2038	5	structure	structure	NN
cord-014597-66vd2mdu	2038	6	differs	differ	VBZ
cord-014597-66vd2mdu	2038	7	depending	depend	VBG
cord-014597-66vd2mdu	2038	8	on	on	IN
cord-014597-66vd2mdu	2038	9	the	the	DT
cord-014597-66vd2mdu	2038	10	location	location	NN
cord-014597-66vd2mdu	2038	11	of	of	IN
cord-014597-66vd2mdu	2038	12	the	the	DT
cord-014597-66vd2mdu	2038	13	chromosomal	chromosomal	JJ
cord-014597-66vd2mdu	2038	14	region	region	NN
cord-014597-66vd2mdu	2038	15	,	,	,
cord-014597-66vd2mdu	2038	16	which	which	WDT
cord-014597-66vd2mdu	2038	17	affects	affect	VBZ
cord-014597-66vd2mdu	2038	18	the	the	DT
cord-014597-66vd2mdu	2038	19	expression	expression	NN
cord-014597-66vd2mdu	2038	20	level	level	NN
cord-014597-66vd2mdu	2038	21	of	of	IN
cord-014597-66vd2mdu	2038	22	the	the	DT
cord-014597-66vd2mdu	2038	23	gene	gene	NN
cord-014597-66vd2mdu	2038	24	of	of	IN
cord-014597-66vd2mdu	2038	25	interest	interest	NN
cord-014597-66vd2mdu	2038	26	[	[	-LRB-
cord-014597-66vd2mdu	2038	27	2	2	CD
cord-014597-66vd2mdu	2038	28	]	]	-RRB-
cord-014597-66vd2mdu	2038	29	.	.	.
cord-014597-66vd2mdu	2039	1	Recently	recently	RB
cord-014597-66vd2mdu	2039	2	,	,	,
cord-014597-66vd2mdu	2039	3	gene	gene	NN
cord-014597-66vd2mdu	2039	4	-	-	HYPH
cord-014597-66vd2mdu	2039	5	targeting	target	VBG
cord-014597-66vd2mdu	2039	6	methods	method	NNS
cord-014597-66vd2mdu	2039	7	that	that	WDT
cord-014597-66vd2mdu	2039	8	enable	enable	VBP
cord-014597-66vd2mdu	2039	9	site	site	NN
cord-014597-66vd2mdu	2039	10	-	-	HYPH
cord-014597-66vd2mdu	2039	11	specific	specific	JJ
cord-014597-66vd2mdu	2039	12	integration	integration	NN
cord-014597-66vd2mdu	2039	13	of	of	IN
cord-014597-66vd2mdu	2039	14	expression	expression	NN
cord-014597-66vd2mdu	2039	15	vectors	vector	NNS
cord-014597-66vd2mdu	2039	16	have	have	VBP
cord-014597-66vd2mdu	2039	17	been	be	VBN
cord-014597-66vd2mdu	2039	18	developed	develop	VBN
cord-014597-66vd2mdu	2039	19	.	.	.
cord-014597-66vd2mdu	2040	1	However	however	RB
cord-014597-66vd2mdu	2040	2	,	,	,
cord-014597-66vd2mdu	2040	3	the	the	DT
cord-014597-66vd2mdu	2040	4	regions	region	NNS
cord-014597-66vd2mdu	2040	5	that	that	WDT
cord-014597-66vd2mdu	2040	6	are	be	VBP
cord-014597-66vd2mdu	2040	7	most	most	RBS
cord-014597-66vd2mdu	2040	8	efficient	efficient	JJ
cord-014597-66vd2mdu	2040	9	for	for	IN
cord-014597-66vd2mdu	2040	10	exogenous	exogenous	JJ
cord-014597-66vd2mdu	2040	11	gene	gene	NN
cord-014597-66vd2mdu	2040	12	expression	expression	NN
cord-014597-66vd2mdu	2040	13	have	have	VBP
cord-014597-66vd2mdu	2040	14	not	not	RB
cord-014597-66vd2mdu	2040	15	been	be	VBN
cord-014597-66vd2mdu	2040	16	clarified	clarify	VBN
cord-014597-66vd2mdu	2040	17	.	.	.
cord-014597-66vd2mdu	2041	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2041	2	previously	previously	RB
cord-014597-66vd2mdu	2041	3	constructed	construct	VBD
cord-014597-66vd2mdu	2041	4	a	a	DT
cord-014597-66vd2mdu	2041	5	CHO	CHO	NNP
cord-014597-66vd2mdu	2041	6	genomic	genomic	JJ
cord-014597-66vd2mdu	2041	7	bacterial	bacterial	JJ
cord-014597-66vd2mdu	2041	8	artificial	artificial	JJ
cord-014597-66vd2mdu	2041	9	chromosome	chromosome	NN
cord-014597-66vd2mdu	2041	10	(	(	-LRB-
cord-014597-66vd2mdu	2041	11	BAC	BAC	NNP
cord-014597-66vd2mdu	2041	12	)	)	-RRB-
cord-014597-66vd2mdu	2041	13	library	library	NN
cord-014597-66vd2mdu	2041	14	generated	generate	VBN
cord-014597-66vd2mdu	2041	15	from	from	IN
cord-014597-66vd2mdu	2041	16	the	the	DT
cord-014597-66vd2mdu	2041	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2041	18	CHO	CHO	NNP
cord-014597-66vd2mdu	2041	19	-	-	HYPH
cord-014597-66vd2mdu	2041	20	DG44	DG44	NNP
cord-014597-66vd2mdu	2041	21	cell	cell	NN
cord-014597-66vd2mdu	2041	22	line	line	NN
cord-014597-66vd2mdu	2041	23	.	.	.
cord-014597-66vd2mdu	2042	1	It	-PRON-	PRP
cord-014597-66vd2mdu	2042	2	was	be	VBD
cord-014597-66vd2mdu	2042	3	expected	expect	VBN
cord-014597-66vd2mdu	2042	4	to	to	TO
cord-014597-66vd2mdu	2042	5	cover	cover	VB
cord-014597-66vd2mdu	2042	6	the	the	DT
cord-014597-66vd2mdu	2042	7	entire	entire	JJ
cord-014597-66vd2mdu	2042	8	CHO	CHO	NNP
cord-014597-66vd2mdu	2042	9	genome	genome	NN
cord-014597-66vd2mdu	2042	10	five	five	CD
cord-014597-66vd2mdu	2042	11	times	time	NNS
cord-014597-66vd2mdu	2042	12	.	.	.
cord-014597-66vd2mdu	2043	1	The	the	DT
cord-014597-66vd2mdu	2043	2	20	20	CD
cord-014597-66vd2mdu	2043	3	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	2043	4	in	in	IN
cord-014597-66vd2mdu	2043	5	CHO	CHO	NNP
cord-014597-66vd2mdu	2043	6	-	-	HYPH
cord-014597-66vd2mdu	2043	7	DG44	DG44	NNP
cord-014597-66vd2mdu	2043	8	cells	cell	NNS
cord-014597-66vd2mdu	2043	9	were	be	VBD
cord-014597-66vd2mdu	2043	10	aligned	align	VBN
cord-014597-66vd2mdu	2043	11	in	in	IN
cord-014597-66vd2mdu	2043	12	decreasing	decrease	VBG
cord-014597-66vd2mdu	2043	13	order	order	NN
cord-014597-66vd2mdu	2043	14	of	of	IN
cord-014597-66vd2mdu	2043	15	size	size	NN
cord-014597-66vd2mdu	2043	16	and	and	CC
cord-014597-66vd2mdu	2043	17	assigned	assign	VBN
cord-014597-66vd2mdu	2043	18	letters	letter	NNS
cord-014597-66vd2mdu	2043	19	from	from	IN
cord-014597-66vd2mdu	2043	20	A	a	NN
cord-014597-66vd2mdu	2043	21	to	to	IN
cord-014597-66vd2mdu	2043	22	T	T	NNP
cord-014597-66vd2mdu	2043	23	[	[	-LRB-
cord-014597-66vd2mdu	2043	24	3	3	CD
cord-014597-66vd2mdu	2043	25	]	]	-RRB-
cord-014597-66vd2mdu	2043	26	.	.	.
cord-014597-66vd2mdu	2044	1	Three	three	CD
cord-014597-66vd2mdu	2044	2	hundred	hundred	CD
cord-014597-66vd2mdu	2044	3	and	and	CC
cord-014597-66vd2mdu	2044	4	four	four	CD
cord-014597-66vd2mdu	2044	5	BAC	BAC	NNP
cord-014597-66vd2mdu	2044	6	clones	clone	NNS
cord-014597-66vd2mdu	2044	7	were	be	VBD
cord-014597-66vd2mdu	2044	8	mapped	map	VBN
cord-014597-66vd2mdu	2044	9	to	to	IN
cord-014597-66vd2mdu	2044	10	every	every	DT
cord-014597-66vd2mdu	2044	11	chromosome	chromosome	NN
cord-014597-66vd2mdu	2044	12	of	of	IN
cord-014597-66vd2mdu	2044	13	CHO	CHO	NNP
cord-014597-66vd2mdu	2044	14	-	-	HYPH
cord-014597-66vd2mdu	2044	15	DG44	DG44	NNP
cord-014597-66vd2mdu	2044	16	.	.	.
cord-014597-66vd2mdu	2045	1	Among	among	IN
cord-014597-66vd2mdu	2045	2	the	the	DT
cord-014597-66vd2mdu	2045	3	karyotypes	karyotype	NNS
cord-014597-66vd2mdu	2045	4	of	of	IN
cord-014597-66vd2mdu	2045	5	CHO	CHO	NNP
cord-014597-66vd2mdu	2045	6	-	-	HYPH
cord-014597-66vd2mdu	2045	7	DG44	DG44	NNP
cord-014597-66vd2mdu	2045	8	,	,	,
cord-014597-66vd2mdu	2045	9	CHO	CHO	NNP
cord-014597-66vd2mdu	2045	10	-	-	HYPH
cord-014597-66vd2mdu	2045	11	K1	K1	NNP
cord-014597-66vd2mdu	2045	12	and	and	CC
cord-014597-66vd2mdu	2045	13	primary	primary	JJ
cord-014597-66vd2mdu	2045	14	Chinese	chinese	JJ
cord-014597-66vd2mdu	2045	15	hamster	hamster	NN
cord-014597-66vd2mdu	2045	16	cells	cell	NNS
cord-014597-66vd2mdu	2045	17	,	,	,
cord-014597-66vd2mdu	2046	1	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	2046	2	A	A	NNP
cord-014597-66vd2mdu	2046	3	and	and	CC
cord-014597-66vd2mdu	2046	4	B	b	NN
cord-014597-66vd2mdu	2046	5	are	be	VBP
cord-014597-66vd2mdu	2046	6	considered	consider	VBN
cord-014597-66vd2mdu	2046	7	as	as	IN
cord-014597-66vd2mdu	2046	8	the	the	DT
cord-014597-66vd2mdu	2046	9	sole	sole	NN
cord-014597-66vd2mdu	2046	10	paired	pair	VBN
cord-014597-66vd2mdu	2046	11	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	2046	12	corresponding	correspond	VBG
cord-014597-66vd2mdu	2046	13	to	to	IN
cord-014597-66vd2mdu	2046	14	chromosome	chromosome	NN
cord-014597-66vd2mdu	2046	15	1	1	CD
cord-014597-66vd2mdu	2046	16	in	in	IN
cord-014597-66vd2mdu	2046	17	primary	primary	JJ
cord-014597-66vd2mdu	2046	18	Chinese	chinese	JJ
cord-014597-66vd2mdu	2046	19	hamster	hamster	NN
cord-014597-66vd2mdu	2046	20	cells	cell	NNS
cord-014597-66vd2mdu	2046	21	.	.	.
cord-014597-66vd2mdu	2047	1	Hence	hence	RB
cord-014597-66vd2mdu	2047	2	,	,	,
cord-014597-66vd2mdu	2047	3	chromosomes	chromosome	VBZ
cord-014597-66vd2mdu	2047	4	A	a	NN
cord-014597-66vd2mdu	2047	5	and	and	CC
cord-014597-66vd2mdu	2047	6	B	b	NN
cord-014597-66vd2mdu	2047	7	are	be	VBP
cord-014597-66vd2mdu	2047	8	considered	consider	VBN
cord-014597-66vd2mdu	2047	9	to	to	TO
cord-014597-66vd2mdu	2047	10	be	be	VB
cord-014597-66vd2mdu	2047	11	stable	stable	JJ
cord-014597-66vd2mdu	2047	12	[	[	-LRB-
cord-014597-66vd2mdu	2047	13	4	4	CD
cord-014597-66vd2mdu	2047	14	]	]	-RRB-
cord-014597-66vd2mdu	2047	15	.	.	.
cord-014597-66vd2mdu	2048	1	In	in	IN
cord-014597-66vd2mdu	2048	2	this	this	DT
cord-014597-66vd2mdu	2048	3	study	study	NN
cord-014597-66vd2mdu	2048	4	,	,	,
cord-014597-66vd2mdu	2048	5	we	-PRON-	PRP
cord-014597-66vd2mdu	2048	6	constructed	construct	VBD
cord-014597-66vd2mdu	2048	7	antibody	antibody	NN
cord-014597-66vd2mdu	2048	8	-	-	HYPH
cord-014597-66vd2mdu	2048	9	producing	produce	VBG
cord-014597-66vd2mdu	2048	10	cells	cell	NNS
cord-014597-66vd2mdu	2048	11	by	by	IN
cord-014597-66vd2mdu	2048	12	using	use	VBG
cord-014597-66vd2mdu	2048	13	a	a	DT
cord-014597-66vd2mdu	2048	14	gene	gene	NN
cord-014597-66vd2mdu	2048	15	-	-	HYPH
cord-014597-66vd2mdu	2048	16	targeting	targeting	NN
cord-014597-66vd2mdu	2048	17	method	method	NN
cord-014597-66vd2mdu	2048	18	,	,	,
cord-014597-66vd2mdu	2048	19	which	which	WDT
cord-014597-66vd2mdu	2048	20	focused	focus	VBD
cord-014597-66vd2mdu	2048	21	on	on	IN
cord-014597-66vd2mdu	2048	22	the	the	DT
cord-014597-66vd2mdu	2048	23	stable	stable	JJ
cord-014597-66vd2mdu	2048	24	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	2048	25	.	.	.
cord-014597-66vd2mdu	2049	1	A	a	DT
cord-014597-66vd2mdu	2049	2	gene	gene	NN
cord-014597-66vd2mdu	2049	3	map	map	NN
cord-014597-66vd2mdu	2049	4	of	of	IN
cord-014597-66vd2mdu	2049	5	chromosome	chromosome	NN
cord-014597-66vd2mdu	2049	6	1	1	CD
cord-014597-66vd2mdu	2049	7	was	be	VBD
cord-014597-66vd2mdu	2049	8	constructed	construct	VBN
cord-014597-66vd2mdu	2049	9	by	by	IN
cord-014597-66vd2mdu	2049	10	combining	combine	VBG
cord-014597-66vd2mdu	2049	11	the	the	DT
cord-014597-66vd2mdu	2049	12	BAC	bac	NN
cord-014597-66vd2mdu	2049	13	-	-	HYPH
cord-014597-66vd2mdu	2049	14	fluorescence	fluorescence	NN
cord-014597-66vd2mdu	2049	15	in	in	IN
cord-014597-66vd2mdu	2049	16	situ	situ	NN
cord-014597-66vd2mdu	2049	17	hybridization	hybridization	NN
cord-014597-66vd2mdu	2049	18	(	(	-LRB-
cord-014597-66vd2mdu	2049	19	FISH)-based	fish)-based	JJ
cord-014597-66vd2mdu	2049	20	chromosome	chromosome	NN
cord-014597-66vd2mdu	2049	21	physical	physical	JJ
cord-014597-66vd2mdu	2049	22	map	map	NN
cord-014597-66vd2mdu	2049	23	and	and	CC
cord-014597-66vd2mdu	2049	24	sequence	sequence	NN
cord-014597-66vd2mdu	2049	25	data	datum	NNS
cord-014597-66vd2mdu	2049	26	of	of	IN
cord-014597-66vd2mdu	2049	27	mapped	map	VBN
cord-014597-66vd2mdu	2049	28	BAC	BAC	NNP
cord-014597-66vd2mdu	2049	29	clones	clone	NNS
cord-014597-66vd2mdu	2049	30	.	.	.
cord-014597-66vd2mdu	2050	1	The	the	DT
cord-014597-66vd2mdu	2050	2	sequences	sequence	NNS
cord-014597-66vd2mdu	2050	3	of	of	IN
cord-014597-66vd2mdu	2050	4	BAC	BAC	NNP
cord-014597-66vd2mdu	2050	5	clones	clone	NNS
cord-014597-66vd2mdu	2050	6	were	be	VBD
cord-014597-66vd2mdu	2050	7	searched	search	VBN
cord-014597-66vd2mdu	2050	8	by	by	IN
cord-014597-66vd2mdu	2050	9	BLAST	blast	NN
cord-014597-66vd2mdu	2050	10	with	with	IN
cord-014597-66vd2mdu	2050	11	NCBI	NCBI	NNP
cord-014597-66vd2mdu	2050	12	and	and	CC
cord-014597-66vd2mdu	2050	13	CHOgenome.org	CHOgenome.org	NNP
cord-014597-66vd2mdu	2050	14	databases	database	NNS
cord-014597-66vd2mdu	2050	15	.	.	.
cord-014597-66vd2mdu	2051	1	Three	three	CD
cord-014597-66vd2mdu	2051	2	different	different	JJ
cord-014597-66vd2mdu	2051	3	regions	region	NNS
cord-014597-66vd2mdu	2051	4	on	on	IN
cord-014597-66vd2mdu	2051	5	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	2051	6	A	a	NN
cord-014597-66vd2mdu	2051	7	and	and	CC
cord-014597-66vd2mdu	2051	8	B	B	NNP
cord-014597-66vd2mdu	2051	9	were	be	VBD
cord-014597-66vd2mdu	2051	10	selected	select	VBN
cord-014597-66vd2mdu	2051	11	based	base	VBN
cord-014597-66vd2mdu	2051	12	on	on	IN
cord-014597-66vd2mdu	2051	13	CHO	CHO	NNP
cord-014597-66vd2mdu	2051	14	genomic	genomic	JJ
cord-014597-66vd2mdu	2051	15	BAC	BAC	NNP
cord-014597-66vd2mdu	2051	16	library	library	NN
cord-014597-66vd2mdu	2051	17	sequences	sequence	NNS
cord-014597-66vd2mdu	2051	18	as	as	IN
cord-014597-66vd2mdu	2051	19	target	target	NN
cord-014597-66vd2mdu	2051	20	sites	site	NNS
cord-014597-66vd2mdu	2051	21	.	.	.
cord-014597-66vd2mdu	2052	1	CHO	CHO	NNP
cord-014597-66vd2mdu	2052	2	-	-	HYPH
cord-014597-66vd2mdu	2052	3	K1	K1	NNP
cord-014597-66vd2mdu	2052	4	cells	cell	NNS
cord-014597-66vd2mdu	2052	5	were	be	VBD
cord-014597-66vd2mdu	2052	6	stably	stably	RB
cord-014597-66vd2mdu	2052	7	transfected	transfecte	VBN
cord-014597-66vd2mdu	2052	8	by	by	IN
cord-014597-66vd2mdu	2052	9	lipofection	lipofection	NN
cord-014597-66vd2mdu	2052	10	.	.	.
cord-014597-66vd2mdu	2053	1	The	the	DT
cord-014597-66vd2mdu	2053	2	target	target	NN
cord-014597-66vd2mdu	2053	3	sequences	sequence	NNS
cord-014597-66vd2mdu	2053	4	were	be	VBD
cord-014597-66vd2mdu	2053	5	broken	break	VBN
cord-014597-66vd2mdu	2053	6	using	use	VBG
cord-014597-66vd2mdu	2053	7	the	the	DT
cord-014597-66vd2mdu	2053	8	clustered	cluster	VBN
cord-014597-66vd2mdu	2053	9	regularly	regularly	RB
cord-014597-66vd2mdu	2053	10	interspaced	interspace	VBD
cord-014597-66vd2mdu	2053	11	short	short	JJ
cord-014597-66vd2mdu	2053	12	palindromic	palindromic	JJ
cord-014597-66vd2mdu	2053	13	repeats	repeat	NNS
cord-014597-66vd2mdu	2054	1	(	(	-LRB-
cord-014597-66vd2mdu	2054	2	CRISPR)/CRISPR	CRISPR)/CRISPR	NNP
cord-014597-66vd2mdu	2054	3	-	-	HYPH
cord-014597-66vd2mdu	2054	4	associated	associate	VBN
cord-014597-66vd2mdu	2054	5	protein	protein	NN
cord-014597-66vd2mdu	2054	6	9	9	CD
cord-014597-66vd2mdu	2054	7	(	(	-LRB-
cord-014597-66vd2mdu	2054	8	Cas9	Cas9	NNP
cord-014597-66vd2mdu	2054	9	)	)	-RRB-
cord-014597-66vd2mdu	2054	10	system	system	NN
cord-014597-66vd2mdu	2054	11	and	and	CC
cord-014597-66vd2mdu	2054	12	humanized	humanize	VBN
cord-014597-66vd2mdu	2054	13	IgG1	IgG1	NNP
cord-014597-66vd2mdu	2054	14	genes	gene	NNS
cord-014597-66vd2mdu	2054	15	were	be	VBD
cord-014597-66vd2mdu	2054	16	integrated	integrate	VBN
cord-014597-66vd2mdu	2054	17	by	by	IN
cord-014597-66vd2mdu	2054	18	non	non	JJ
cord-014597-66vd2mdu	2054	19	-	-	JJ
cord-014597-66vd2mdu	2054	20	homologous	homologous	JJ
cord-014597-66vd2mdu	2054	21	end	end	NN
cord-014597-66vd2mdu	2054	22	joining	join	VBG
cord-014597-66vd2mdu	2054	23	recombination	recombination	NN
cord-014597-66vd2mdu	2054	24	.	.	.
cord-014597-66vd2mdu	2055	1	Transfection	transfection	NN
cord-014597-66vd2mdu	2055	2	without	without	IN
cord-014597-66vd2mdu	2055	3	using	use	VBG
cord-014597-66vd2mdu	2055	4	the	the	DT
cord-014597-66vd2mdu	2055	5	CRISPR/	CRISPR/	NNP
cord-014597-66vd2mdu	2055	6	Cas9	Cas9	NNP
cord-014597-66vd2mdu	2055	7	system	system	NN
cord-014597-66vd2mdu	2055	8	was	be	VBD
cord-014597-66vd2mdu	2055	9	also	also	RB
cord-014597-66vd2mdu	2055	10	performed	perform	VBN
cord-014597-66vd2mdu	2055	11	.	.	.
cord-014597-66vd2mdu	2056	1	These	these	DT
cord-014597-66vd2mdu	2056	2	cell	cell	NN
cord-014597-66vd2mdu	2056	3	pools	pool	NNS
cord-014597-66vd2mdu	2056	4	were	be	VBD
cord-014597-66vd2mdu	2056	5	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2056	6	for	for	IN
cord-014597-66vd2mdu	2056	7	six	six	CD
cord-014597-66vd2mdu	2056	8	days	day	NNS
cord-014597-66vd2mdu	2056	9	with	with	IN
cord-014597-66vd2mdu	2056	10	serum	serum	NN
cord-014597-66vd2mdu	2056	11	-	-	HYPH
cord-014597-66vd2mdu	2056	12	supplemented	supplement	VBN
cord-014597-66vd2mdu	2056	13	medium	medium	NN
cord-014597-66vd2mdu	2056	14	,	,	,
cord-014597-66vd2mdu	2056	15	and	and	CC
cord-014597-66vd2mdu	2056	16	their	-PRON-	PRP$
cord-014597-66vd2mdu	2056	17	levels	level	NNS
cord-014597-66vd2mdu	2056	18	of	of	IN
cord-014597-66vd2mdu	2056	19	antibody	antibody	NN
cord-014597-66vd2mdu	2056	20	productivity	productivity	NN
cord-014597-66vd2mdu	2056	21	were	be	VBD
cord-014597-66vd2mdu	2056	22	evaluated	evaluate	VBN
cord-014597-66vd2mdu	2056	23	by	by	IN
cord-014597-66vd2mdu	2056	24	ELISA	ELISA	NNP
cord-014597-66vd2mdu	2056	25	.	.	.
cord-014597-66vd2mdu	2057	1	Copy	copy	JJ
cord-014597-66vd2mdu	2057	2	number	number	NN
cord-014597-66vd2mdu	2057	3	analysis	analysis	NN
cord-014597-66vd2mdu	2057	4	was	be	VBD
cord-014597-66vd2mdu	2057	5	also	also	RB
cord-014597-66vd2mdu	2057	6	performed	perform	VBN
cord-014597-66vd2mdu	2057	7	using	use	VBG
cord-014597-66vd2mdu	2057	8	real	real	JJ
cord-014597-66vd2mdu	2057	9	-	-	HYPH
cord-014597-66vd2mdu	2057	10	time	time	NN
cord-014597-66vd2mdu	2057	11	PCR	PCR	NNP
cord-014597-66vd2mdu	2057	12	.	.	.
cord-014597-66vd2mdu	2058	1	Results	result	NNS
cord-014597-66vd2mdu	2058	2	and	and	CC
cord-014597-66vd2mdu	2058	3	discussion	discussion	NN
cord-014597-66vd2mdu	2058	4	Construction	construction	NN
cord-014597-66vd2mdu	2058	5	of	of	IN
cord-014597-66vd2mdu	2058	6	gene	gene	NN
cord-014597-66vd2mdu	2058	7	map	map	NN
cord-014597-66vd2mdu	2058	8	of	of	IN
cord-014597-66vd2mdu	2058	9	chromosome	chromosome	NN
cord-014597-66vd2mdu	2058	10	1	1	CD
cord-014597-66vd2mdu	2058	11	:	:	:
cord-014597-66vd2mdu	2059	1	Eighty	eighty	CD
cord-014597-66vd2mdu	2059	2	-	-	HYPH
cord-014597-66vd2mdu	2059	3	three	three	CD
cord-014597-66vd2mdu	2059	4	BAC	BAC	NNP
cord-014597-66vd2mdu	2059	5	clones	clone	NNS
cord-014597-66vd2mdu	2059	6	were	be	VBD
cord-014597-66vd2mdu	2059	7	mapped	map	VBN
cord-014597-66vd2mdu	2059	8	onto	onto	IN
cord-014597-66vd2mdu	2059	9	chromosomes	chromosome	NNS
cord-014597-66vd2mdu	2059	10	A	a	NN
cord-014597-66vd2mdu	2059	11	and	and	CC
cord-014597-66vd2mdu	2059	12	B	b	NN
cord-014597-66vd2mdu	2059	13	(	(	-LRB-
cord-014597-66vd2mdu	2059	14	each	each	DT
cord-014597-66vd2mdu	2059	15	clone	clone	NN
cord-014597-66vd2mdu	2059	16	contained	contain	VBD
cord-014597-66vd2mdu	2059	17	100	100	CD
cord-014597-66vd2mdu	2059	18	-	-	SYM
cord-014597-66vd2mdu	2059	19	150	150	CD
cord-014597-66vd2mdu	2059	20	kb	kb	NNS
cord-014597-66vd2mdu	2059	21	of	of	IN
cord-014597-66vd2mdu	2059	22	the	the	DT
cord-014597-66vd2mdu	2059	23	CHO	CHO	NNP
cord-014597-66vd2mdu	2059	24	genome	genome	NN
cord-014597-66vd2mdu	2059	25	sequence	sequence	NN
cord-014597-66vd2mdu	2059	26	)	)	-RRB-
cord-014597-66vd2mdu	2059	27	.	.	.
cord-014597-66vd2mdu	2060	1	As	as	IN
cord-014597-66vd2mdu	2060	2	a	a	DT
cord-014597-66vd2mdu	2060	3	result	result	NN
cord-014597-66vd2mdu	2060	4	of	of	IN
cord-014597-66vd2mdu	2060	5	annotations	annotation	NNS
cord-014597-66vd2mdu	2060	6	of	of	IN
cord-014597-66vd2mdu	2060	7	83	83	CD
cord-014597-66vd2mdu	2060	8	BAC	BAC	NNP
cord-014597-66vd2mdu	2060	9	clone	clone	NN
cord-014597-66vd2mdu	2060	10	sequences	sequence	NNS
cord-014597-66vd2mdu	2060	11	,	,	,
cord-014597-66vd2mdu	2060	12	91	91	CD
cord-014597-66vd2mdu	2060	13	genes	gene	NNS
cord-014597-66vd2mdu	2060	14	were	be	VBD
cord-014597-66vd2mdu	2060	15	mapped	map	VBN
cord-014597-66vd2mdu	2060	16	on	on	IN
cord-014597-66vd2mdu	2060	17	chromosome	chromosome	NN
cord-014597-66vd2mdu	2060	18	1	1	CD
cord-014597-66vd2mdu	2060	19	.	.	.
cord-014597-66vd2mdu	2061	1	Investigation	investigation	NN
cord-014597-66vd2mdu	2061	2	of	of	IN
cord-014597-66vd2mdu	2061	3	the	the	DT
cord-014597-66vd2mdu	2061	4	differences	difference	NNS
cord-014597-66vd2mdu	2061	5	of	of	IN
cord-014597-66vd2mdu	2061	6	productivity	productivity	NN
cord-014597-66vd2mdu	2061	7	among	among	IN
cord-014597-66vd2mdu	2061	8	antibodyproducing	antibodyproduce	VBG
cord-014597-66vd2mdu	2061	9	cells	cell	NNS
cord-014597-66vd2mdu	2061	10	that	that	WDT
cord-014597-66vd2mdu	2061	11	were	be	VBD
cord-014597-66vd2mdu	2061	12	constructed	construct	VBN
cord-014597-66vd2mdu	2061	13	by	by	IN
cord-014597-66vd2mdu	2061	14	chromosome	chromosome	NN
cord-014597-66vd2mdu	2061	15	1	1	CD
cord-014597-66vd2mdu	2061	16	targeting	targeting	NN
cord-014597-66vd2mdu	2061	17	and/or	and/or	CC
cord-014597-66vd2mdu	2061	18	random	random	JJ
cord-014597-66vd2mdu	2061	19	integration	integration	NN
cord-014597-66vd2mdu	2061	20	:	:	:
cord-014597-66vd2mdu	2061	21	Cell	cell	NN
cord-014597-66vd2mdu	2061	22	growth	growth	NN
cord-014597-66vd2mdu	2061	23	was	be	VBD
cord-014597-66vd2mdu	2061	24	not	not	RB
cord-014597-66vd2mdu	2061	25	affected	affect	VBN
cord-014597-66vd2mdu	2061	26	by	by	IN
cord-014597-66vd2mdu	2061	27	the	the	DT
cord-014597-66vd2mdu	2061	28	gene	gene	NN
cord-014597-66vd2mdu	2061	29	targeting	target	VBG
cord-014597-66vd2mdu	2061	30	site	site	NN
cord-014597-66vd2mdu	2061	31	.	.	.
cord-014597-66vd2mdu	2062	1	The	the	DT
cord-014597-66vd2mdu	2062	2	specific	specific	JJ
cord-014597-66vd2mdu	2062	3	production	production	NN
cord-014597-66vd2mdu	2062	4	rates	rate	NNS
cord-014597-66vd2mdu	2062	5	of	of	IN
cord-014597-66vd2mdu	2062	6	antibodyproducing	antibodyproduce	VBG
cord-014597-66vd2mdu	2062	7	cell	cell	NN
cord-014597-66vd2mdu	2062	8	pools	pool	NNS
cord-014597-66vd2mdu	2062	9	constructed	construct	VBN
cord-014597-66vd2mdu	2062	10	by	by	IN
cord-014597-66vd2mdu	2062	11	gene	gene	NN
cord-014597-66vd2mdu	2062	12	targeting	targeting	NN
cord-014597-66vd2mdu	2062	13	of	of	IN
cord-014597-66vd2mdu	2062	14	chromosome	chromosome	NN
cord-014597-66vd2mdu	2062	15	1	1	CD
cord-014597-66vd2mdu	2062	16	were	be	VBD
cord-014597-66vd2mdu	2062	17	higher	high	JJR
cord-014597-66vd2mdu	2062	18	than	than	IN
cord-014597-66vd2mdu	2062	19	those	those	DT
cord-014597-66vd2mdu	2062	20	of	of	IN
cord-014597-66vd2mdu	2062	21	the	the	DT
cord-014597-66vd2mdu	2062	22	cell	cell	NN
cord-014597-66vd2mdu	2062	23	pool	pool	NN
cord-014597-66vd2mdu	2062	24	constructed	construct	VBN
cord-014597-66vd2mdu	2062	25	by	by	IN
cord-014597-66vd2mdu	2062	26	random	random	JJ
cord-014597-66vd2mdu	2062	27	integration	integration	NN
cord-014597-66vd2mdu	2062	28	.	.	.
cord-014597-66vd2mdu	2063	1	All	all	DT
cord-014597-66vd2mdu	2063	2	cell	cell	NN
cord-014597-66vd2mdu	2063	3	pools	pool	NNS
cord-014597-66vd2mdu	2063	4	constructed	construct	VBN
cord-014597-66vd2mdu	2063	5	by	by	IN
cord-014597-66vd2mdu	2063	6	gene	gene	NN
cord-014597-66vd2mdu	2063	7	targeting	targeting	NN
cord-014597-66vd2mdu	2063	8	showed	show	VBD
cord-014597-66vd2mdu	2063	9	lower	low	JJR
cord-014597-66vd2mdu	2063	10	copy	copy	NN
cord-014597-66vd2mdu	2063	11	numbers	number	NNS
cord-014597-66vd2mdu	2063	12	of	of	IN
cord-014597-66vd2mdu	2063	13	heavy	heavy	JJ
cord-014597-66vd2mdu	2063	14	chain	chain	NN
cord-014597-66vd2mdu	2063	15	and	and	CC
cord-014597-66vd2mdu	2063	16	light	light	JJ
cord-014597-66vd2mdu	2063	17	chain	chain	NN
cord-014597-66vd2mdu	2063	18	in	in	IN
cord-014597-66vd2mdu	2063	19	genomic	genomic	JJ
cord-014597-66vd2mdu	2063	20	DNA	dna	NN
cord-014597-66vd2mdu	2063	21	than	than	IN
cord-014597-66vd2mdu	2063	22	those	those	DT
cord-014597-66vd2mdu	2063	23	in	in	IN
cord-014597-66vd2mdu	2063	24	the	the	DT
cord-014597-66vd2mdu	2063	25	cell	cell	NN
cord-014597-66vd2mdu	2063	26	pool	pool	NN
cord-014597-66vd2mdu	2063	27	constructed	construct	VBN
cord-014597-66vd2mdu	2063	28	by	by	IN
cord-014597-66vd2mdu	2063	29	random	random	JJ
cord-014597-66vd2mdu	2063	30	integration	integration	NN
cord-014597-66vd2mdu	2063	31	,	,	,
cord-014597-66vd2mdu	2063	32	despite	despite	IN
cord-014597-66vd2mdu	2063	33	showing	show	VBG
cord-014597-66vd2mdu	2063	34	high	high	JJ
cord-014597-66vd2mdu	2063	35	productivity	productivity	NN
cord-014597-66vd2mdu	2063	36	.	.	.
cord-014597-66vd2mdu	2064	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	2064	2	results	result	NNS
cord-014597-66vd2mdu	2064	3	indicate	indicate	VBP
cord-014597-66vd2mdu	2064	4	that	that	IN
cord-014597-66vd2mdu	2064	5	high	high	JJ
cord-014597-66vd2mdu	2064	6	productivity	productivity	NN
cord-014597-66vd2mdu	2064	7	of	of	IN
cord-014597-66vd2mdu	2064	8	the	the	DT
cord-014597-66vd2mdu	2064	9	cells	cell	NNS
cord-014597-66vd2mdu	2064	10	constructed	construct	VBN
cord-014597-66vd2mdu	2064	11	by	by	IN
cord-014597-66vd2mdu	2064	12	gene	gene	NN
cord-014597-66vd2mdu	2064	13	targeting	targeting	NN
cord-014597-66vd2mdu	2064	14	of	of	IN
cord-014597-66vd2mdu	2064	15	chromosome	chromosome	NN
cord-014597-66vd2mdu	2064	16	1	1	CD
cord-014597-66vd2mdu	2064	17	does	do	VBZ
cord-014597-66vd2mdu	2064	18	not	not	RB
cord-014597-66vd2mdu	2064	19	depend	depend	VB
cord-014597-66vd2mdu	2064	20	on	on	IN
cord-014597-66vd2mdu	2064	21	the	the	DT
cord-014597-66vd2mdu	2064	22	increase	increase	NN
cord-014597-66vd2mdu	2064	23	of	of	IN
cord-014597-66vd2mdu	2064	24	the	the	DT
cord-014597-66vd2mdu	2064	25	antibody	antibody	NN
cord-014597-66vd2mdu	2064	26	copy	copy	NN
cord-014597-66vd2mdu	2064	27	number	number	NN
cord-014597-66vd2mdu	2064	28	,	,	,
cord-014597-66vd2mdu	2064	29	and	and	CC
cord-014597-66vd2mdu	2064	30	that	that	IN
cord-014597-66vd2mdu	2064	31	the	the	DT
cord-014597-66vd2mdu	2064	32	environments	environment	NNS
cord-014597-66vd2mdu	2064	33	around	around	IN
cord-014597-66vd2mdu	2064	34	these	these	DT
cord-014597-66vd2mdu	2064	35	target	target	NN
cord-014597-66vd2mdu	2064	36	regions	region	NNS
cord-014597-66vd2mdu	2064	37	are	be	VBP
cord-014597-66vd2mdu	2064	38	suitable	suitable	JJ
cord-014597-66vd2mdu	2064	39	for	for	IN
cord-014597-66vd2mdu	2064	40	exogenous	exogenous	JJ
cord-014597-66vd2mdu	2064	41	gene	gene	NN
cord-014597-66vd2mdu	2064	42	expression	expression	NN
cord-014597-66vd2mdu	2064	43	.	.	.
cord-014597-66vd2mdu	2065	1	The	the	DT
cord-014597-66vd2mdu	2065	2	approach	approach	NN
cord-014597-66vd2mdu	2065	3	of	of	IN
cord-014597-66vd2mdu	2065	4	using	use	VBG
cord-014597-66vd2mdu	2065	5	gene	gene	NN
cord-014597-66vd2mdu	2065	6	targeting	target	VBG
cord-014597-66vd2mdu	2065	7	to	to	IN
cord-014597-66vd2mdu	2065	8	chromosome	chromosome	NN
cord-014597-66vd2mdu	2065	9	1	1	CD
cord-014597-66vd2mdu	2065	10	may	may	MD
cord-014597-66vd2mdu	2065	11	be	be	VB
cord-014597-66vd2mdu	2065	12	promising	promise	VBG
cord-014597-66vd2mdu	2065	13	for	for	IN
cord-014597-66vd2mdu	2065	14	constructing	construct	VBG
cord-014597-66vd2mdu	2065	15	antibodyproducing	antibodyproduce	VBG
cord-014597-66vd2mdu	2065	16	cells	cell	NNS
cord-014597-66vd2mdu	2065	17	.	.	.
cord-014597-66vd2mdu	2066	1	Retroviral	retroviral	NN
cord-014597-66vd2mdu	2066	2	vectors	vector	NNS
cord-014597-66vd2mdu	2066	3	have	have	VBP
cord-014597-66vd2mdu	2066	4	been	be	VBN
cord-014597-66vd2mdu	2066	5	widely	widely	RB
cord-014597-66vd2mdu	2066	6	used	use	VBN
cord-014597-66vd2mdu	2066	7	as	as	IN
cord-014597-66vd2mdu	2066	8	gene	gene	NN
cord-014597-66vd2mdu	2066	9	delivery	delivery	NN
cord-014597-66vd2mdu	2066	10	tools	tool	NNS
cord-014597-66vd2mdu	2066	11	in	in	IN
cord-014597-66vd2mdu	2066	12	various	various	JJ
cord-014597-66vd2mdu	2066	13	biotechnology	biotechnology	NN
cord-014597-66vd2mdu	2066	14	fields	field	NNS
cord-014597-66vd2mdu	2066	15	.	.	.
cord-014597-66vd2mdu	2067	1	However	however	RB
cord-014597-66vd2mdu	2067	2	,	,	,
cord-014597-66vd2mdu	2067	3	the	the	DT
cord-014597-66vd2mdu	2067	4	random	random	JJ
cord-014597-66vd2mdu	2067	5	integration	integration	NN
cord-014597-66vd2mdu	2067	6	feature	feature	NN
cord-014597-66vd2mdu	2067	7	of	of	IN
cord-014597-66vd2mdu	2067	8	retroviral	retroviral	JJ
cord-014597-66vd2mdu	2067	9	vectors	vector	NNS
cord-014597-66vd2mdu	2067	10	seems	seem	VBZ
cord-014597-66vd2mdu	2067	11	to	to	TO
cord-014597-66vd2mdu	2067	12	cause	cause	VB
cord-014597-66vd2mdu	2067	13	problems	problem	NNS
cord-014597-66vd2mdu	2067	14	such	such	JJ
cord-014597-66vd2mdu	2067	15	as	as	IN
cord-014597-66vd2mdu	2067	16	insertional	insertional	JJ
cord-014597-66vd2mdu	2067	17	mutagenesis	mutagenesis	NN
cord-014597-66vd2mdu	2067	18	and	and	CC
cord-014597-66vd2mdu	2067	19	gene	gene	NN
cord-014597-66vd2mdu	2067	20	silencing	silencing	NN
cord-014597-66vd2mdu	2067	21	.	.	.
cord-014597-66vd2mdu	2068	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2068	2	previously	previously	RB
cord-014597-66vd2mdu	2068	3	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	2068	4	Cre	Cre	NNP
cord-014597-66vd2mdu	2068	5	-	-	HYPH
cord-014597-66vd2mdu	2068	6	mediated	mediate	VBN
cord-014597-66vd2mdu	2068	7	retroviral	retroviral	NN
cord-014597-66vd2mdu	2068	8	transgene	transgene	NN
cord-014597-66vd2mdu	2068	9	insertion	insertion	NN
cord-014597-66vd2mdu	2068	10	into	into	IN
cord-014597-66vd2mdu	2068	11	a	a	DT
cord-014597-66vd2mdu	2068	12	pre	pre	JJ
cord-014597-66vd2mdu	2068	13	-	-	JJ
cord-014597-66vd2mdu	2068	14	determined	determined	JJ
cord-014597-66vd2mdu	2068	15	site	site	NN
cord-014597-66vd2mdu	2068	16	of	of	IN
cord-014597-66vd2mdu	2068	17	the	the	DT
cord-014597-66vd2mdu	2068	18	founder	founder	NN
cord-014597-66vd2mdu	2068	19	cells	cell	NNS
cord-014597-66vd2mdu	2068	20	using	use	VBG
cord-014597-66vd2mdu	2068	21	integrasedefective	integrasedefective	JJ
cord-014597-66vd2mdu	2068	22	retroviral	retroviral	NN
cord-014597-66vd2mdu	2068	23	vectors	vector	NNS
cord-014597-66vd2mdu	2068	24	(	(	-LRB-
cord-014597-66vd2mdu	2068	25	IDRVs	IDRVs	NNP
cord-014597-66vd2mdu	2068	26	)	)	-RRB-
cord-014597-66vd2mdu	2068	27	,	,	,
cord-014597-66vd2mdu	2068	28	where	where	WRB
cord-014597-66vd2mdu	2068	29	a	a	DT
cord-014597-66vd2mdu	2068	30	Cre	cre	JJ
cord-014597-66vd2mdu	2068	31	expression	expression	NN
cord-014597-66vd2mdu	2068	32	plasmid	plasmid	NN
cord-014597-66vd2mdu	2068	33	was	be	VBD
cord-014597-66vd2mdu	2068	34	transfected	transfecte	VBN
cord-014597-66vd2mdu	2068	35	into	into	IN
cord-014597-66vd2mdu	2068	36	the	the	DT
cord-014597-66vd2mdu	2068	37	cells	cell	NNS
cord-014597-66vd2mdu	2068	38	prior	prior	RB
cord-014597-66vd2mdu	2068	39	to	to	IN
cord-014597-66vd2mdu	2068	40	retroviral	retroviral	JJ
cord-014597-66vd2mdu	2068	41	transduction	transduction	NN
cord-014597-66vd2mdu	2068	42	[	[	-LRB-
cord-014597-66vd2mdu	2068	43	1	1	CD
cord-014597-66vd2mdu	2068	44	]	]	-RRB-
cord-014597-66vd2mdu	2068	45	.	.	.
cord-014597-66vd2mdu	2069	1	Recently	recently	RB
cord-014597-66vd2mdu	2069	2	,	,	,
cord-014597-66vd2mdu	2069	3	we	-PRON-	PRP
cord-014597-66vd2mdu	2069	4	reported	report	VBD
cord-014597-66vd2mdu	2069	5	novel	novel	JJ
cord-014597-66vd2mdu	2069	6	hybrid	hybrid	NN
cord-014597-66vd2mdu	2069	7	IDRVs	idrv	NNS
cord-014597-66vd2mdu	2069	8	(	(	-LRB-
cord-014597-66vd2mdu	2069	9	Cre	Cre	NNP
cord-014597-66vd2mdu	2069	10	-	-	HYPH
cord-014597-66vd2mdu	2069	11	IDRVs	IDRVs	NNP
cord-014597-66vd2mdu	2069	12	)	)	-RRB-
cord-014597-66vd2mdu	2070	1	incorporating	incorporate	VBG
cord-014597-66vd2mdu	2070	2	bioactive	bioactive	JJ
cord-014597-66vd2mdu	2070	3	Cre	Cre	NNP
cord-014597-66vd2mdu	2070	4	recombinase	recombinase	NN
cord-014597-66vd2mdu	2070	5	protein	protein	NN
cord-014597-66vd2mdu	2070	6	,	,	,
cord-014597-66vd2mdu	2070	7	and	and	CC
cord-014597-66vd2mdu	2070	8	validated	validate	VBD
cord-014597-66vd2mdu	2070	9	site	site	NN
cord-014597-66vd2mdu	2070	10	-	-	HYPH
cord-014597-66vd2mdu	2070	11	specific	specific	JJ
cord-014597-66vd2mdu	2070	12	gene	gene	NN
cord-014597-66vd2mdu	2070	13	integration	integration	NN
cord-014597-66vd2mdu	2070	14	of	of	IN
cord-014597-66vd2mdu	2070	15	an	an	DT
cord-014597-66vd2mdu	2070	16	scFv	scFv	NNP
cord-014597-66vd2mdu	2070	17	-	-	HYPH
cord-014597-66vd2mdu	2070	18	Fc	Fc	NNP
cord-014597-66vd2mdu	2070	19	antibody	antibody	NN
cord-014597-66vd2mdu	2070	20	expression	expression	NN
cord-014597-66vd2mdu	2070	21	unit	unit	NN
cord-014597-66vd2mdu	2070	22	into	into	IN
cord-014597-66vd2mdu	2070	23	the	the	DT
cord-014597-66vd2mdu	2070	24	Chinese	chinese	JJ
cord-014597-66vd2mdu	2070	25	hamster	hamster	NN
cord-014597-66vd2mdu	2070	26	ovary	ovary	NN
cord-014597-66vd2mdu	2070	27	(	(	-LRB-
cord-014597-66vd2mdu	2070	28	CHO	CHO	NNP
cord-014597-66vd2mdu	2070	29	)	)	-RRB-
cord-014597-66vd2mdu	2070	30	cell	cell	NN
cord-014597-66vd2mdu	2070	31	genome	genome	NN
cord-014597-66vd2mdu	2070	32	[	[	-LRB-
cord-014597-66vd2mdu	2070	33	2	2	CD
cord-014597-66vd2mdu	2070	34	]	]	-RRB-
cord-014597-66vd2mdu	2070	35	.	.	.
cord-014597-66vd2mdu	2071	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2071	2	also	also	RB
cord-014597-66vd2mdu	2071	3	developed	develop	VBD
cord-014597-66vd2mdu	2071	4	an	an	DT
cord-014597-66vd2mdu	2071	5	accumulative	accumulative	JJ
cord-014597-66vd2mdu	2071	6	site	site	NN
cord-014597-66vd2mdu	2071	7	-	-	HYPH
cord-014597-66vd2mdu	2071	8	specific	specific	JJ
cord-014597-66vd2mdu	2071	9	gene	gene	NN
cord-014597-66vd2mdu	2071	10	integration	integration	NN
cord-014597-66vd2mdu	2071	11	system	system	NN
cord-014597-66vd2mdu	2071	12	,	,	,
cord-014597-66vd2mdu	2071	13	which	which	WDT
cord-014597-66vd2mdu	2071	14	enables	enable	VBZ
cord-014597-66vd2mdu	2071	15	repeated	repeat	VBN
cord-014597-66vd2mdu	2071	16	integration	integration	NN
cord-014597-66vd2mdu	2071	17	of	of	IN
cord-014597-66vd2mdu	2071	18	multiple	multiple	JJ
cord-014597-66vd2mdu	2071	19	transgenes	transgene	NNS
cord-014597-66vd2mdu	2071	20	into	into	IN
cord-014597-66vd2mdu	2071	21	a	a	DT
cord-014597-66vd2mdu	2071	22	pre	pre	JJ
cord-014597-66vd2mdu	2071	23	-	-	JJ
cord-014597-66vd2mdu	2071	24	determined	determined	JJ
cord-014597-66vd2mdu	2071	25	locus	locus	NN
cord-014597-66vd2mdu	2071	26	of	of	IN
cord-014597-66vd2mdu	2071	27	the	the	DT
cord-014597-66vd2mdu	2071	28	cell	cell	NN
cord-014597-66vd2mdu	2071	29	genome	genome	NN
cord-014597-66vd2mdu	2071	30	[	[	-LRB-
cord-014597-66vd2mdu	2071	31	3	3	CD
cord-014597-66vd2mdu	2071	32	]	]	-RRB-
cord-014597-66vd2mdu	2071	33	.	.	.
cord-014597-66vd2mdu	2072	1	Here	here	RB
cord-014597-66vd2mdu	2072	2	,	,	,
cord-014597-66vd2mdu	2072	3	we	-PRON-	PRP
cord-014597-66vd2mdu	2072	4	attempted	attempt	VBD
cord-014597-66vd2mdu	2072	5	repeated	repeated	JJ
cord-014597-66vd2mdu	2072	6	integration	integration	NN
cord-014597-66vd2mdu	2072	7	of	of	IN
cord-014597-66vd2mdu	2072	8	transgenes	transgene	NNS
cord-014597-66vd2mdu	2072	9	using	use	VBG
cord-014597-66vd2mdu	2072	10	Cre	Cre	NNP
cord-014597-66vd2mdu	2072	11	-	-	HYPH
cord-014597-66vd2mdu	2072	12	IDRVs	IDRVs	NNP
cord-014597-66vd2mdu	2072	13	.	.	.
cord-014597-66vd2mdu	2073	1	A	a	DT
cord-014597-66vd2mdu	2073	2	viral	viral	JJ
cord-014597-66vd2mdu	2073	3	vector	vector	NN
cord-014597-66vd2mdu	2073	4	plasmid	plasmid	NN
cord-014597-66vd2mdu	2073	5	(	(	-LRB-
cord-014597-66vd2mdu	2073	6	pQMSCV	pQMSCV	NNP
cord-014597-66vd2mdu	2073	7	/	/	SYM
cord-014597-66vd2mdu	2073	8	HD[scFv	HD[scFv	NNP
cord-014597-66vd2mdu	2073	9	-	-	HYPH
cord-014597-66vd2mdu	2073	10	Fc	Fc	NNP
cord-014597-66vd2mdu	2073	11	]	]	-RRB-
cord-014597-66vd2mdu	2073	12	)	)	-RRB-
cord-014597-66vd2mdu	2074	1	encoding	encode	VBG
cord-014597-66vd2mdu	2074	2	reporter	reporter	NN
cord-014597-66vd2mdu	2074	3	genes	gene	NNS
cord-014597-66vd2mdu	2074	4	and	and	CC
cord-014597-66vd2mdu	2074	5	an	an	DT
cord-014597-66vd2mdu	2074	6	scFv	scFv	NNP
cord-014597-66vd2mdu	2074	7	-	-	HYPH
cord-014597-66vd2mdu	2074	8	Fc	Fc	NNP
cord-014597-66vd2mdu	2074	9	expression	expression	NN
cord-014597-66vd2mdu	2074	10	unit	unit	NN
cord-014597-66vd2mdu	2074	11	flanked	flank	VBN
cord-014597-66vd2mdu	2074	12	with	with	IN
cord-014597-66vd2mdu	2074	13	wild	wild	JJ
cord-014597-66vd2mdu	2074	14	-	-	HYPH
cord-014597-66vd2mdu	2074	15	type	type	NN
cord-014597-66vd2mdu	2074	16	and	and	CC
cord-014597-66vd2mdu	2074	17	mutant	mutant	JJ
cord-014597-66vd2mdu	2074	18	loxPs	loxp	NNS
cord-014597-66vd2mdu	2074	19	was	be	VBD
cord-014597-66vd2mdu	2074	20	constructed	construct	VBN
cord-014597-66vd2mdu	2074	21	for	for	IN
cord-014597-66vd2mdu	2074	22	the	the	DT
cord-014597-66vd2mdu	2074	23	production	production	NN
cord-014597-66vd2mdu	2074	24	of	of	IN
cord-014597-66vd2mdu	2074	25	IDRVs	idrvs	PRP
cord-014597-66vd2mdu	2074	26	.	.	.
cord-014597-66vd2mdu	2075	1	Cre	Cre	NNP
cord-014597-66vd2mdu	2075	2	-	-	HYPH
cord-014597-66vd2mdu	2075	3	IDRVs	idrv	NNS
cord-014597-66vd2mdu	2075	4	were	be	VBD
cord-014597-66vd2mdu	2075	5	produced	produce	VBN
cord-014597-66vd2mdu	2075	6	described	describe	VBN
cord-014597-66vd2mdu	2075	7	previously	previously	RB
cord-014597-66vd2mdu	2075	8	[	[	-LRB-
cord-014597-66vd2mdu	2075	9	2	2	CD
cord-014597-66vd2mdu	2075	10	]	]	-RRB-
cord-014597-66vd2mdu	2075	11	.	.	.
cord-014597-66vd2mdu	2076	1	Results	result	NNS
cord-014597-66vd2mdu	2076	2	and	and	CC
cord-014597-66vd2mdu	2076	3	discussion	discussion	NN
cord-014597-66vd2mdu	2076	4	Figure	figure	NN
cord-014597-66vd2mdu	2076	5	1a	1a	NN
cord-014597-66vd2mdu	2076	6	shows	show	VBZ
cord-014597-66vd2mdu	2076	7	a	a	DT
cord-014597-66vd2mdu	2076	8	schematic	schematic	JJ
cord-014597-66vd2mdu	2076	9	drawing	drawing	NN
cord-014597-66vd2mdu	2076	10	of	of	IN
cord-014597-66vd2mdu	2076	11	each	each	DT
cord-014597-66vd2mdu	2076	12	round	round	NN
cord-014597-66vd2mdu	2076	13	of	of	IN
cord-014597-66vd2mdu	2076	14	targeted	target	VBN
cord-014597-66vd2mdu	2076	15	transgene	transgene	NN
cord-014597-66vd2mdu	2076	16	integration	integration	NN
cord-014597-66vd2mdu	2076	17	using	use	VBG
cord-014597-66vd2mdu	2076	18	Cre	Cre	NNP
cord-014597-66vd2mdu	2076	19	-	-	HYPH
cord-014597-66vd2mdu	2076	20	IDRVs	IDRVs	NNP
cord-014597-66vd2mdu	2076	21	harboring	harbor	VBG
cord-014597-66vd2mdu	2076	22	an	an	DT
cord-014597-66vd2mdu	2076	23	scFv	scFv	NNP
cord-014597-66vd2mdu	2076	24	-	-	HYPH
cord-014597-66vd2mdu	2076	25	Fc	Fc	NNP
cord-014597-66vd2mdu	2076	26	expression	expression	NN
cord-014597-66vd2mdu	2076	27	unit	unit	NN
cord-014597-66vd2mdu	2076	28	.	.	.
cord-014597-66vd2mdu	2077	1	(	(	-LRB-
cord-014597-66vd2mdu	2077	2	Fig	Fig	NNP
cord-014597-66vd2mdu	2077	3	.	.	.
cord-014597-66vd2mdu	2077	4	1b	1b	CD
cord-014597-66vd2mdu	2077	5	)	)	-RRB-
cord-014597-66vd2mdu	2077	6	.	.	.
cord-014597-66vd2mdu	2078	1	Genomic	genomic	JJ
cord-014597-66vd2mdu	2078	2	DNA	dna	NN
cord-014597-66vd2mdu	2078	3	extracted	extract	VBN
cord-014597-66vd2mdu	2078	4	from	from	IN
cord-014597-66vd2mdu	2078	5	the	the	DT
cord-014597-66vd2mdu	2078	6	cells	cell	NNS
cord-014597-66vd2mdu	2078	7	were	be	VBD
cord-014597-66vd2mdu	2078	8	subjected	subject	VBN
cord-014597-66vd2mdu	2078	9	to	to	IN
cord-014597-66vd2mdu	2078	10	PCR	PCR	NNP
cord-014597-66vd2mdu	2078	11	using	use	VBG
cord-014597-66vd2mdu	2078	12	specific	specific	JJ
cord-014597-66vd2mdu	2078	13	primer	primer	NN
cord-014597-66vd2mdu	2078	14	pairs	pair	NNS
cord-014597-66vd2mdu	2078	15	α	α	NN
cord-014597-66vd2mdu	2078	16	and	and	CC
cord-014597-66vd2mdu	2078	17	β	β	NN
cord-014597-66vd2mdu	2078	18	,	,	,
cord-014597-66vd2mdu	2078	19	and	and	CC
cord-014597-66vd2mdu	2078	20	γ	γ	NN
cord-014597-66vd2mdu	2078	21	and	and	CC
cord-014597-66vd2mdu	2078	22	δ	δ	NN
cord-014597-66vd2mdu	2078	23	to	to	TO
cord-014597-66vd2mdu	2078	24	confirm	confirm	VB
cord-014597-66vd2mdu	2078	25	site	site	NN
cord-014597-66vd2mdu	2078	26	-	-	HYPH
cord-014597-66vd2mdu	2078	27	specific	specific	JJ
cord-014597-66vd2mdu	2078	28	integration	integration	NN
cord-014597-66vd2mdu	2078	29	.	.	.
cord-014597-66vd2mdu	2079	1	DNA	dna	NN
cord-014597-66vd2mdu	2079	2	fragments	fragment	NNS
cord-014597-66vd2mdu	2079	3	with	with	IN
cord-014597-66vd2mdu	2079	4	expected	expect	VBN
cord-014597-66vd2mdu	2079	5	sizes	size	NNS
cord-014597-66vd2mdu	2079	6	were	be	VBD
cord-014597-66vd2mdu	2079	7	amplified	amplify	VBN
cord-014597-66vd2mdu	2079	8	in	in	IN
cord-014597-66vd2mdu	2079	9	each	each	DT
cord-014597-66vd2mdu	2079	10	cell	cell	NN
cord-014597-66vd2mdu	2079	11	clone	clone	NN
cord-014597-66vd2mdu	2079	12	(	(	-LRB-
cord-014597-66vd2mdu	2079	13	Fig	Fig	NNP
cord-014597-66vd2mdu	2079	14	.	.	.
cord-014597-66vd2mdu	2079	15	1c	1c	CD
cord-014597-66vd2mdu	2079	16	)	)	-RRB-
cord-014597-66vd2mdu	2079	17	.	.	.
cord-014597-66vd2mdu	2080	1	These	these	DT
cord-014597-66vd2mdu	2080	2	results	result	NNS
cord-014597-66vd2mdu	2080	3	indicate	indicate	VBP
cord-014597-66vd2mdu	2080	4	that	that	IN
cord-014597-66vd2mdu	2080	5	site	site	NN
cord-014597-66vd2mdu	2080	6	-	-	HYPH
cord-014597-66vd2mdu	2080	7	specific	specific	JJ
cord-014597-66vd2mdu	2080	8	repeated	repeat	VBN
cord-014597-66vd2mdu	2080	9	integration	integration	NN
cord-014597-66vd2mdu	2080	10	was	be	VBD
cord-014597-66vd2mdu	2080	11	achieved	achieve	VBN
cord-014597-66vd2mdu	2080	12	using	use	VBG
cord-014597-66vd2mdu	2080	13	Cre	Cre	NNP
cord-014597-66vd2mdu	2080	14	-	-	HYPH
cord-014597-66vd2mdu	2080	15	IDRVs	IDRVs	NNP
cord-014597-66vd2mdu	2080	16	.	.	.
cord-014597-66vd2mdu	2081	1	In	in	IN
cord-014597-66vd2mdu	2081	2	contrast	contrast	NN
cord-014597-66vd2mdu	2081	3	,	,	,
cord-014597-66vd2mdu	2081	4	scFv	scFv	NNP
cord-014597-66vd2mdu	2081	5	-	-	HYPH
cord-014597-66vd2mdu	2081	6	Fc	Fc	NNP
cord-014597-66vd2mdu	2081	7	productivity	productivity	NN
cord-014597-66vd2mdu	2081	8	in	in	IN
cord-014597-66vd2mdu	2081	9	CHO	CHO	NNP
cord-014597-66vd2mdu	2081	10	/	/	SYM
cord-014597-66vd2mdu	2081	11	HD[scFv	HD[scFv	NNP
cord-014597-66vd2mdu	2081	12	-	-	HYPH
cord-014597-66vd2mdu	2081	13	Fc]×2	Fc]×2	NNP
cord-014597-66vd2mdu	2081	14	cells	cell	NNS
cord-014597-66vd2mdu	2081	15	was	be	VBD
cord-014597-66vd2mdu	2081	16	slightly	slightly	RB
cord-014597-66vd2mdu	2081	17	decreased	decrease	VBN
cord-014597-66vd2mdu	2081	18	compared	compare	VBN
cord-014597-66vd2mdu	2081	19	with	with	IN
cord-014597-66vd2mdu	2081	20	that	that	DT
cord-014597-66vd2mdu	2081	21	of	of	IN
cord-014597-66vd2mdu	2081	22	CHO	CHO	NNP
cord-014597-66vd2mdu	2081	23	/	/	SYM
cord-014597-66vd2mdu	2081	24	NE[scFv	NE[scFv	NNP
cord-014597-66vd2mdu	2082	1	-Fc]×1	-Fc]×1	NFP
cord-014597-66vd2mdu	2082	2	(	(	-LRB-
cord-014597-66vd2mdu	2082	3	data	datum	NNS
cord-014597-66vd2mdu	2082	4	not	not	RB
cord-014597-66vd2mdu	2082	5	shown	show	VBN
cord-014597-66vd2mdu	2082	6	)	)	-RRB-
cord-014597-66vd2mdu	2082	7	.	.	.
cord-014597-66vd2mdu	2083	1	Although	although	IN
cord-014597-66vd2mdu	2083	2	the	the	DT
cord-014597-66vd2mdu	2083	3	reason	reason	NN
cord-014597-66vd2mdu	2083	4	remains	remain	VBZ
cord-014597-66vd2mdu	2083	5	unclear	unclear	JJ
cord-014597-66vd2mdu	2083	6	,	,	,
cord-014597-66vd2mdu	2083	7	repeat	repeat	VB
cord-014597-66vd2mdu	2083	8	-	-	HYPH
cord-014597-66vd2mdu	2083	9	induced	induce	VBN
cord-014597-66vd2mdu	2083	10	gene	gene	NN
cord-014597-66vd2mdu	2083	11	silencing	silencing	NN
cord-014597-66vd2mdu	2083	12	might	may	MD
cord-014597-66vd2mdu	2083	13	occur	occur	VB
cord-014597-66vd2mdu	2083	14	due	due	IN
cord-014597-66vd2mdu	2083	15	to	to	TO
cord-014597-66vd2mdu	2083	16	tandem	tandem	JJ
cord-014597-66vd2mdu	2083	17	repeat	repeat	NN
cord-014597-66vd2mdu	2083	18	structure	structure	NN
cord-014597-66vd2mdu	2083	19	of	of	IN
cord-014597-66vd2mdu	2083	20	expression	expression	NN
cord-014597-66vd2mdu	2083	21	units	unit	NNS
cord-014597-66vd2mdu	2083	22	.	.	.
cord-014597-66vd2mdu	2084	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2084	2	reported	report	VBD
cord-014597-66vd2mdu	2084	3	improved	improve	VBN
cord-014597-66vd2mdu	2084	4	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2084	5	antibody	antibody	NN
cord-014597-66vd2mdu	2084	6	production	production	NN
cord-014597-66vd2mdu	2084	7	using	use	VBG
cord-014597-66vd2mdu	2084	8	a	a	DT
cord-014597-66vd2mdu	2084	9	production	production	NN
cord-014597-66vd2mdu	2084	10	enhancer	enhancer	NN
cord-014597-66vd2mdu	2084	11	element	element	NN
cord-014597-66vd2mdu	2084	12	[	[	-LRB-
cord-014597-66vd2mdu	2084	13	4	4	CD
cord-014597-66vd2mdu	2084	14	]	]	-RRB-
cord-014597-66vd2mdu	2084	15	.	.	.
cord-014597-66vd2mdu	2085	1	Such	such	JJ
cord-014597-66vd2mdu	2085	2	a	a	DT
cord-014597-66vd2mdu	2085	3	cis	cis	JJ
cord-014597-66vd2mdu	2085	4	-	-	HYPH
cord-014597-66vd2mdu	2085	5	regulatory	regulatory	JJ
cord-014597-66vd2mdu	2085	6	element	element	NN
cord-014597-66vd2mdu	2085	7	might	may	MD
cord-014597-66vd2mdu	2085	8	be	be	VB
cord-014597-66vd2mdu	2085	9	a	a	DT
cord-014597-66vd2mdu	2085	10	feasible	feasible	JJ
cord-014597-66vd2mdu	2085	11	approach	approach	NN
cord-014597-66vd2mdu	2085	12	to	to	TO
cord-014597-66vd2mdu	2085	13	enhance	enhance	VB
cord-014597-66vd2mdu	2085	14	the	the	DT
cord-014597-66vd2mdu	2085	15	productivity	productivity	NN
cord-014597-66vd2mdu	2085	16	.	.	.
cord-014597-66vd2mdu	2086	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2086	2	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	2086	3	site	site	NN
cord-014597-66vd2mdu	2086	4	-	-	HYPH
cord-014597-66vd2mdu	2086	5	specific	specific	JJ
cord-014597-66vd2mdu	2086	6	repeated	repeat	VBN
cord-014597-66vd2mdu	2086	7	transgene	transgene	NN
cord-014597-66vd2mdu	2086	8	integration	integration	NN
cord-014597-66vd2mdu	2086	9	into	into	IN
cord-014597-66vd2mdu	2086	10	a	a	DT
cord-014597-66vd2mdu	2086	11	pre	pre	JJ
cord-014597-66vd2mdu	2086	12	-	-	JJ
cord-014597-66vd2mdu	2086	13	determined	determined	JJ
cord-014597-66vd2mdu	2086	14	chromosomal	chromosomal	JJ
cord-014597-66vd2mdu	2086	15	locus	locus	NN
cord-014597-66vd2mdu	2086	16	using	use	VBG
cord-014597-66vd2mdu	2086	17	Cre	Cre	NNP
cord-014597-66vd2mdu	2086	18	-	-	HYPH
cord-014597-66vd2mdu	2086	19	IDRVs	IDRVs	NNP
cord-014597-66vd2mdu	2086	20	for	for	IN
cord-014597-66vd2mdu	2086	21	the	the	DT
cord-014597-66vd2mdu	2086	22	production	production	NN
cord-014597-66vd2mdu	2086	23	of	of	IN
cord-014597-66vd2mdu	2086	24	an	an	DT
cord-014597-66vd2mdu	2086	25	scFv	scFv	NNP
cord-014597-66vd2mdu	2086	26	-	-	HYPH
cord-014597-66vd2mdu	2086	27	Fc	Fc	NNP
cord-014597-66vd2mdu	2086	28	antibody	antibody	NN
cord-014597-66vd2mdu	2086	29	.	.	.
cord-014597-66vd2mdu	2087	1	If	if	IN
cord-014597-66vd2mdu	2087	2	lipids	lipid	NNS
cord-014597-66vd2mdu	2087	3	role	role	NN
cord-014597-66vd2mdu	2087	4	in	in	IN
cord-014597-66vd2mdu	2087	5	the	the	DT
cord-014597-66vd2mdu	2087	6	cell	cell	NN
cord-014597-66vd2mdu	2087	7	have	have	VBP
cord-014597-66vd2mdu	2087	8	been	be	VBN
cord-014597-66vd2mdu	2087	9	reduced	reduce	VBN
cord-014597-66vd2mdu	2087	10	for	for	IN
cord-014597-66vd2mdu	2087	11	a	a	DT
cord-014597-66vd2mdu	2087	12	long	long	JJ
cord-014597-66vd2mdu	2087	13	time	time	NN
cord-014597-66vd2mdu	2087	14	to	to	IN
cord-014597-66vd2mdu	2087	15	cell	cell	NN
cord-014597-66vd2mdu	2087	16	membrane	membrane	NN
cord-014597-66vd2mdu	2087	17	formation	formation	NN
cord-014597-66vd2mdu	2087	18	,	,	,
cord-014597-66vd2mdu	2087	19	it	-PRON-	PRP
cord-014597-66vd2mdu	2087	20	is	be	VBZ
cord-014597-66vd2mdu	2087	21	now	now	RB
cord-014597-66vd2mdu	2087	22	understood	understand	VBN
cord-014597-66vd2mdu	2087	23	that	that	IN
cord-014597-66vd2mdu	2087	24	lipids	lipid	NNS
cord-014597-66vd2mdu	2087	25	plays	play	VBZ
cord-014597-66vd2mdu	2087	26	also	also	RB
cord-014597-66vd2mdu	2087	27	a	a	DT
cord-014597-66vd2mdu	2087	28	role	role	NN
cord-014597-66vd2mdu	2087	29	into	into	IN
cord-014597-66vd2mdu	2087	30	energy	energy	NN
cord-014597-66vd2mdu	2087	31	metabolism	metabolism	NN
cord-014597-66vd2mdu	2087	32	,	,	,
cord-014597-66vd2mdu	2087	33	vesicular	vesicular	JJ
cord-014597-66vd2mdu	2087	34	transport	transport	NN
cord-014597-66vd2mdu	2087	35	,	,	,
cord-014597-66vd2mdu	2087	36	membrane	membrane	NN
cord-014597-66vd2mdu	2087	37	structure	structure	NN
cord-014597-66vd2mdu	2087	38	,	,	,
cord-014597-66vd2mdu	2087	39	dynamics	dynamic	NNS
cord-014597-66vd2mdu	2087	40	and	and	CC
cord-014597-66vd2mdu	2087	41	signaling	signaling	NN
cord-014597-66vd2mdu	2087	42	.	.	.
cord-014597-66vd2mdu	2088	1	However	however	RB
cord-014597-66vd2mdu	2088	2	,	,	,
cord-014597-66vd2mdu	2088	3	the	the	DT
cord-014597-66vd2mdu	2088	4	exact	exact	JJ
cord-014597-66vd2mdu	2088	5	mechanism	mechanism	NN
cord-014597-66vd2mdu	2088	6	of	of	IN
cord-014597-66vd2mdu	2088	7	how	how	WRB
cord-014597-66vd2mdu	2088	8	compositional	compositional	JJ
cord-014597-66vd2mdu	2088	9	complexity	complexity	NN
cord-014597-66vd2mdu	2088	10	affects	affect	VBZ
cord-014597-66vd2mdu	2088	11	cell	cell	NN
cord-014597-66vd2mdu	2088	12	homeostasis	homeostasis	NN
cord-014597-66vd2mdu	2088	13	remains	remain	VBZ
cord-014597-66vd2mdu	2088	14	unclear	unclear	JJ
cord-014597-66vd2mdu	2088	15	.	.	.
cord-014597-66vd2mdu	2089	1	Thanks	thank	NNS
cord-014597-66vd2mdu	2089	2	to	to	IN
cord-014597-66vd2mdu	2089	3	recent	recent	JJ
cord-014597-66vd2mdu	2089	4	advances	advance	NNS
cord-014597-66vd2mdu	2089	5	in	in	IN
cord-014597-66vd2mdu	2089	6	mass	mass	NN
cord-014597-66vd2mdu	2089	7	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	2089	8	,	,	,
cord-014597-66vd2mdu	2089	9	it	-PRON-	PRP
cord-014597-66vd2mdu	2089	10	is	be	VBZ
cord-014597-66vd2mdu	2089	11	now	now	RB
cord-014597-66vd2mdu	2089	12	possible	possible	JJ
cord-014597-66vd2mdu	2089	13	to	to	TO
cord-014597-66vd2mdu	2089	14	study	study	VB
cord-014597-66vd2mdu	2089	15	a	a	DT
cord-014597-66vd2mdu	2089	16	wide	wide	JJ
cord-014597-66vd2mdu	2089	17	range	range	NN
cord-014597-66vd2mdu	2089	18	of	of	IN
cord-014597-66vd2mdu	2089	19	lipids	lipid	NNS
cord-014597-66vd2mdu	2089	20	,	,	,
cord-014597-66vd2mdu	2089	21	providing	provide	VBG
cord-014597-66vd2mdu	2089	22	a	a	DT
cord-014597-66vd2mdu	2089	23	better	well	JJR
cord-014597-66vd2mdu	2089	24	understanding	understanding	NN
cord-014597-66vd2mdu	2089	25	of	of	IN
cord-014597-66vd2mdu	2089	26	lipid	lipid	NN
cord-014597-66vd2mdu	2089	27	homeostasis	homeostasis	NN
cord-014597-66vd2mdu	2089	28	in	in	IN
cord-014597-66vd2mdu	2089	29	high	high	JJ
cord-014597-66vd2mdu	2089	30	performance	performance	NN
cord-014597-66vd2mdu	2089	31	cell	cell	NN
cord-014597-66vd2mdu	2089	32	culture	culture	NN
cord-014597-66vd2mdu	2089	33	processes	process	NNS
cord-014597-66vd2mdu	2089	34	.	.	.
cord-014597-66vd2mdu	2090	1	The	the	DT
cord-014597-66vd2mdu	2090	2	purpose	purpose	NN
cord-014597-66vd2mdu	2090	3	of	of	IN
cord-014597-66vd2mdu	2090	4	this	this	DT
cord-014597-66vd2mdu	2090	5	work	work	NN
cord-014597-66vd2mdu	2090	6	was	be	VBD
cord-014597-66vd2mdu	2090	7	to	to	TO
cord-014597-66vd2mdu	2090	8	develop	develop	VB
cord-014597-66vd2mdu	2090	9	a	a	DT
cord-014597-66vd2mdu	2090	10	robust	robust	JJ
cord-014597-66vd2mdu	2090	11	lipidomics	lipidomic	NNS
cord-014597-66vd2mdu	2090	12	method	method	NN
cord-014597-66vd2mdu	2090	13	applied	apply	VBN
cord-014597-66vd2mdu	2090	14	to	to	IN
cord-014597-66vd2mdu	2090	15	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2090	16	cell	cell	NN
cord-014597-66vd2mdu	2090	17	cultures	culture	NNS
cord-014597-66vd2mdu	2090	18	in	in	IN
cord-014597-66vd2mdu	2090	19	a	a	DT
cord-014597-66vd2mdu	2090	20	three	three	CD
cord-014597-66vd2mdu	2090	21	step	step	NN
cord-014597-66vd2mdu	2090	22	method	method	NN
cord-014597-66vd2mdu	2090	23	:	:	:
cord-014597-66vd2mdu	2090	24	extraction	extraction	NN
cord-014597-66vd2mdu	2090	25	,	,	,
cord-014597-66vd2mdu	2090	26	separation	separation	NN
cord-014597-66vd2mdu	2090	27	and	and	CC
cord-014597-66vd2mdu	2090	28	detection	detection	NN
cord-014597-66vd2mdu	2090	29	(	(	-LRB-
cord-014597-66vd2mdu	2090	30	Fig	Fig	NNP
cord-014597-66vd2mdu	2090	31	.	.	.
cord-014597-66vd2mdu	2090	32	1	1	CD
cord-014597-66vd2mdu	2090	33	)	)	-RRB-
cord-014597-66vd2mdu	2090	34	.	.	.
cord-014597-66vd2mdu	2091	1	Both	both	DT
cord-014597-66vd2mdu	2091	2	Matyash	Matyash	NNP
cord-014597-66vd2mdu	2091	3	[	[	-LRB-
cord-014597-66vd2mdu	2091	4	1	1	CD
cord-014597-66vd2mdu	2091	5	]	]	-RRB-
cord-014597-66vd2mdu	2091	6	and	and	CC
cord-014597-66vd2mdu	2091	7	Folch	Folch	NNP
cord-014597-66vd2mdu	2091	8	[	[	-LRB-
cord-014597-66vd2mdu	2091	9	2	2	CD
cord-014597-66vd2mdu	2091	10	]	]	-RRB-
cord-014597-66vd2mdu	2091	11	extraction	extraction	NN
cord-014597-66vd2mdu	2091	12	method	method	NN
cord-014597-66vd2mdu	2091	13	were	be	VBD
cord-014597-66vd2mdu	2091	14	performed	perform	VBN
cord-014597-66vd2mdu	2091	15	on	on	IN
cord-014597-66vd2mdu	2091	16	our	-PRON-	PRP$
cord-014597-66vd2mdu	2091	17	cells	cell	NNS
cord-014597-66vd2mdu	2091	18	to	to	TO
cord-014597-66vd2mdu	2091	19	reach	reach	VB
cord-014597-66vd2mdu	2091	20	the	the	DT
cord-014597-66vd2mdu	2091	21	highest	high	JJS
cord-014597-66vd2mdu	2091	22	yield	yield	NN
cord-014597-66vd2mdu	2091	23	.	.	.
cord-014597-66vd2mdu	2092	1	Two	two	CD
cord-014597-66vd2mdu	2092	2	separation	separation	NN
cord-014597-66vd2mdu	2092	3	techniques	technique	NNS
cord-014597-66vd2mdu	2092	4	were	be	VBD
cord-014597-66vd2mdu	2092	5	also	also	RB
cord-014597-66vd2mdu	2092	6	tested	test	VBN
cord-014597-66vd2mdu	2092	7	:	:	:
cord-014597-66vd2mdu	2092	8	hydrophilic	hydrophilic	JJ
cord-014597-66vd2mdu	2092	9	interaction	interaction	NN
cord-014597-66vd2mdu	2092	10	liquid	liquid	NN
cord-014597-66vd2mdu	2092	11	chromatography	chromatography	NN
cord-014597-66vd2mdu	2092	12	(	(	-LRB-
cord-014597-66vd2mdu	2092	13	HILIC	HILIC	NNP
cord-014597-66vd2mdu	2092	14	)	)	-RRB-
cord-014597-66vd2mdu	2092	15	and	and	CC
cord-014597-66vd2mdu	2092	16	reverse	reverse	VB
cord-014597-66vd2mdu	2092	17	phase	phase	NN
cord-014597-66vd2mdu	2092	18	chromatography	chromatography	NN
cord-014597-66vd2mdu	2092	19	.	.	.
cord-014597-66vd2mdu	2093	1	Finally	finally	RB
cord-014597-66vd2mdu	2093	2	lipid	lipid	NN
cord-014597-66vd2mdu	2093	3	classes	class	NNS
cord-014597-66vd2mdu	2093	4	'	'	POS
cord-014597-66vd2mdu	2093	5	identification	identification	NN
cord-014597-66vd2mdu	2093	6	was	be	VBD
cord-014597-66vd2mdu	2093	7	achieved	achieve	VBN
cord-014597-66vd2mdu	2093	8	by	by	IN
cord-014597-66vd2mdu	2093	9	tandem	tandem	JJ
cord-014597-66vd2mdu	2093	10	mass	mass	NN
cord-014597-66vd2mdu	2093	11	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	2093	12	analysis	analysis	NN
cord-014597-66vd2mdu	2093	13	thanks	thank	NNS
cord-014597-66vd2mdu	2093	14	to	to	IN
cord-014597-66vd2mdu	2093	15	structure	structure	NN
cord-014597-66vd2mdu	2093	16	-	-	HYPH
cord-014597-66vd2mdu	2093	17	specific	specific	JJ
cord-014597-66vd2mdu	2093	18	fragmentation	fragmentation	NN
cord-014597-66vd2mdu	2093	19	ions	ion	NNS
cord-014597-66vd2mdu	2093	20	.	.	.
cord-014597-66vd2mdu	2094	1	The	the	DT
cord-014597-66vd2mdu	2094	2	yield	yield	NN
cord-014597-66vd2mdu	2094	3	obtained	obtain	VBN
cord-014597-66vd2mdu	2094	4	with	with	IN
cord-014597-66vd2mdu	2094	5	Matyash	Matyash	NNP
cord-014597-66vd2mdu	2094	6	extraction	extraction	NN
cord-014597-66vd2mdu	2094	7	method	method	NN
cord-014597-66vd2mdu	2094	8	was	be	VBD
cord-014597-66vd2mdu	2094	9	higher	high	JJR
cord-014597-66vd2mdu	2094	10	than	than	IN
cord-014597-66vd2mdu	2094	11	with	with	IN
cord-014597-66vd2mdu	2094	12	Folch	Folch	NNP
cord-014597-66vd2mdu	2094	13	method	method	NN
cord-014597-66vd2mdu	2094	14	for	for	IN
cord-014597-66vd2mdu	2094	15	each	each	DT
cord-014597-66vd2mdu	2094	16	lipid	lipid	NN
cord-014597-66vd2mdu	2094	17	class	class	NN
cord-014597-66vd2mdu	2094	18	tested	test	VBN
cord-014597-66vd2mdu	2094	19	.	.	.
cord-014597-66vd2mdu	2095	1	Besides	besides	RB
cord-014597-66vd2mdu	2095	2	,	,	,
cord-014597-66vd2mdu	2095	3	Matyash	Matyash	NNP
cord-014597-66vd2mdu	2095	4	method	method	NN
cord-014597-66vd2mdu	2095	5	presents	present	VBZ
cord-014597-66vd2mdu	2095	6	also	also	RB
cord-014597-66vd2mdu	2095	7	the	the	DT
cord-014597-66vd2mdu	2095	8	advantage	advantage	NN
cord-014597-66vd2mdu	2095	9	to	to	TO
cord-014597-66vd2mdu	2095	10	be	be	VB
cord-014597-66vd2mdu	2095	11	less	less	RBR
cord-014597-66vd2mdu	2095	12	toxic	toxic	JJ
cord-014597-66vd2mdu	2095	13	and	and	CC
cord-014597-66vd2mdu	2095	14	suitable	suitable	JJ
cord-014597-66vd2mdu	2095	15	for	for	IN
cord-014597-66vd2mdu	2095	16	high	high	JJ
cord-014597-66vd2mdu	2095	17	throughput	throughput	NN
cord-014597-66vd2mdu	2095	18	analysis	analysis	NN
cord-014597-66vd2mdu	2095	19	since	since	IN
cord-014597-66vd2mdu	2095	20	the	the	DT
cord-014597-66vd2mdu	2095	21	organic	organic	JJ
cord-014597-66vd2mdu	2095	22	layer	layer	NN
cord-014597-66vd2mdu	2095	23	is	be	VBZ
cord-014597-66vd2mdu	2095	24	above	above	IN
cord-014597-66vd2mdu	2095	25	the	the	DT
cord-014597-66vd2mdu	2095	26	aqueous	aqueous	JJ
cord-014597-66vd2mdu	2095	27	layer	layer	NN
cord-014597-66vd2mdu	2095	28	.	.	.
cord-014597-66vd2mdu	2096	1	Lipids	lipid	NNS
cord-014597-66vd2mdu	2096	2	separation	separation	NN
cord-014597-66vd2mdu	2096	3	by	by	IN
cord-014597-66vd2mdu	2096	4	HILIC	HILIC	NNP
cord-014597-66vd2mdu	2096	5	is	be	VBZ
cord-014597-66vd2mdu	2096	6	based	base	VBN
cord-014597-66vd2mdu	2096	7	on	on	IN
cord-014597-66vd2mdu	2096	8	their	-PRON-	PRP$
cord-014597-66vd2mdu	2096	9	polar	polar	JJ
cord-014597-66vd2mdu	2096	10	head	head	NN
cord-014597-66vd2mdu	2096	11	.	.	.
cord-014597-66vd2mdu	2097	1	Since	since	IN
cord-014597-66vd2mdu	2097	2	lipid	lipid	NN
cord-014597-66vd2mdu	2097	3	classes	class	NNS
cord-014597-66vd2mdu	2097	4	are	be	VBP
cord-014597-66vd2mdu	2097	5	defined	define	VBN
cord-014597-66vd2mdu	2097	6	by	by	IN
cord-014597-66vd2mdu	2097	7	polar	polar	JJ
cord-014597-66vd2mdu	2097	8	head	head	NN
cord-014597-66vd2mdu	2097	9	,	,	,
cord-014597-66vd2mdu	2097	10	the	the	DT
cord-014597-66vd2mdu	2097	11	lipids	lipid	NNS
cord-014597-66vd2mdu	2097	12	are	be	VBP
cord-014597-66vd2mdu	2097	13	eluted	elute	VBN
cord-014597-66vd2mdu	2097	14	class	class	NN
cord-014597-66vd2mdu	2097	15	by	by	IN
cord-014597-66vd2mdu	2097	16	class	class	NN
cord-014597-66vd2mdu	2097	17	,	,	,
cord-014597-66vd2mdu	2097	18	making	make	VBG
cord-014597-66vd2mdu	2097	19	their	-PRON-	PRP$
cord-014597-66vd2mdu	2097	20	identification	identification	NN
cord-014597-66vd2mdu	2097	21	easier	easy	JJR
cord-014597-66vd2mdu	2097	22	.	.	.
cord-014597-66vd2mdu	2098	1	The	the	DT
cord-014597-66vd2mdu	2098	2	separation	separation	NN
cord-014597-66vd2mdu	2098	3	of	of	IN
cord-014597-66vd2mdu	2098	4	lipids	lipid	NNS
cord-014597-66vd2mdu	2098	5	by	by	IN
cord-014597-66vd2mdu	2098	6	reverse	reverse	JJ
cord-014597-66vd2mdu	2098	7	phase	phase	NN
cord-014597-66vd2mdu	2098	8	was	be	VBD
cord-014597-66vd2mdu	2098	9	correct	correct	JJ
cord-014597-66vd2mdu	2098	10	but	but	CC
cord-014597-66vd2mdu	2098	11	the	the	DT
cord-014597-66vd2mdu	2098	12	method	method	NN
cord-014597-66vd2mdu	2098	13	is	be	VBZ
cord-014597-66vd2mdu	2098	14	longer	long	JJR
cord-014597-66vd2mdu	2098	15	and	and	CC
cord-014597-66vd2mdu	2098	16	we	-PRON-	PRP
cord-014597-66vd2mdu	2098	17	observed	observe	VBD
cord-014597-66vd2mdu	2098	18	a	a	DT
cord-014597-66vd2mdu	2098	19	massive	massive	JJ
cord-014597-66vd2mdu	2098	20	carryover	carryover	NN
cord-014597-66vd2mdu	2098	21	of	of	IN
cord-014597-66vd2mdu	2098	22	triglycerides	triglyceride	NNS
cord-014597-66vd2mdu	2098	23	on	on	IN
cord-014597-66vd2mdu	2098	24	the	the	DT
cord-014597-66vd2mdu	2098	25	column	column	NN
cord-014597-66vd2mdu	2098	26	.	.	.
cord-014597-66vd2mdu	2099	1	Finally	finally	RB
cord-014597-66vd2mdu	2099	2	each	each	DT
cord-014597-66vd2mdu	2099	3	lipid	lipid	NN
cord-014597-66vd2mdu	2099	4	class	class	NN
cord-014597-66vd2mdu	2099	5	was	be	VBD
cord-014597-66vd2mdu	2099	6	screened	screen	VBN
cord-014597-66vd2mdu	2099	7	in	in	IN
cord-014597-66vd2mdu	2099	8	MS	MS	NNP
cord-014597-66vd2mdu	2099	9	/	/	SYM
cord-014597-66vd2mdu	2099	10	MS	MS	NNP
cord-014597-66vd2mdu	2099	11	parent	parent	NN
cord-014597-66vd2mdu	2099	12	ion	ion	NN
cord-014597-66vd2mdu	2099	13	mode	mode	NN
cord-014597-66vd2mdu	2099	14	.	.	.
cord-014597-66vd2mdu	2100	1	Target	Target	NNP
cord-014597-66vd2mdu	2100	2	daughter	daughter	NN
cord-014597-66vd2mdu	2100	3	ion	ion	NN
cord-014597-66vd2mdu	2100	4	was	be	VBD
cord-014597-66vd2mdu	2100	5	set	set	VBN
cord-014597-66vd2mdu	2100	6	according	accord	VBG
cord-014597-66vd2mdu	2100	7	to	to	IN
cord-014597-66vd2mdu	2100	8	the	the	DT
cord-014597-66vd2mdu	2100	9	lipid	lipid	NN
cord-014597-66vd2mdu	2100	10	class	class	NN
cord-014597-66vd2mdu	2100	11	structure	structure	NN
cord-014597-66vd2mdu	2100	12	and	and	CC
cord-014597-66vd2mdu	2100	13	fragmentation	fragmentation	NN
cord-014597-66vd2mdu	2100	14	pattern	pattern	NN
cord-014597-66vd2mdu	2100	15	.	.	.
cord-014597-66vd2mdu	2101	1	This	this	DT
cord-014597-66vd2mdu	2101	2	detection	detection	NN
cord-014597-66vd2mdu	2101	3	technique	technique	NN
cord-014597-66vd2mdu	2101	4	enabled	enable	VBD
cord-014597-66vd2mdu	2101	5	the	the	DT
cord-014597-66vd2mdu	2101	6	identification	identification	NN
cord-014597-66vd2mdu	2101	7	of	of	IN
cord-014597-66vd2mdu	2101	8	50	50	CD
cord-014597-66vd2mdu	2101	9	different	different	JJ
cord-014597-66vd2mdu	2101	10	lipids	lipid	NNS
cord-014597-66vd2mdu	2101	11	.	.	.
cord-014597-66vd2mdu	2102	1	To	to	TO
cord-014597-66vd2mdu	2102	2	ensure	ensure	VB
cord-014597-66vd2mdu	2102	3	the	the	DT
cord-014597-66vd2mdu	2102	4	absolute	absolute	JJ
cord-014597-66vd2mdu	2102	5	quantification	quantification	NN
cord-014597-66vd2mdu	2102	6	of	of	IN
cord-014597-66vd2mdu	2102	7	the	the	DT
cord-014597-66vd2mdu	2102	8	detected	detect	VBN
cord-014597-66vd2mdu	2102	9	lipids	lipid	NNS
cord-014597-66vd2mdu	2102	10	and	and	CC
cord-014597-66vd2mdu	2102	11	to	to	TO
cord-014597-66vd2mdu	2102	12	guarantee	guarantee	VB
cord-014597-66vd2mdu	2102	13	comparable	comparable	JJ
cord-014597-66vd2mdu	2102	14	results	result	NNS
cord-014597-66vd2mdu	2102	15	between	between	IN
cord-014597-66vd2mdu	2102	16	batches	batch	NNS
cord-014597-66vd2mdu	2102	17	labeled	label	VBN
cord-014597-66vd2mdu	2102	18	internal	internal	JJ
cord-014597-66vd2mdu	2102	19	standard	standard	NN
cord-014597-66vd2mdu	2102	20	were	be	VBD
cord-014597-66vd2mdu	2102	21	added	add	VBN
cord-014597-66vd2mdu	2102	22	prior	prior	RB
cord-014597-66vd2mdu	2102	23	to	to	IN
cord-014597-66vd2mdu	2102	24	extraction	extraction	NN
cord-014597-66vd2mdu	2102	25	.	.	.
cord-014597-66vd2mdu	2103	1	This	this	DT
cord-014597-66vd2mdu	2103	2	method	method	NN
cord-014597-66vd2mdu	2103	3	was	be	VBD
cord-014597-66vd2mdu	2103	4	optimized	optimize	VBN
cord-014597-66vd2mdu	2103	5	in	in	IN
cord-014597-66vd2mdu	2103	6	a	a	DT
cord-014597-66vd2mdu	2103	7	stepwise	stepwise	JJ
cord-014597-66vd2mdu	2103	8	process	process	NN
cord-014597-66vd2mdu	2103	9	to	to	TO
cord-014597-66vd2mdu	2103	10	ensure	ensure	VB
cord-014597-66vd2mdu	2103	11	a	a	DT
cord-014597-66vd2mdu	2103	12	sensitive	sensitive	JJ
cord-014597-66vd2mdu	2103	13	and	and	CC
cord-014597-66vd2mdu	2103	14	selective	selective	JJ
cord-014597-66vd2mdu	2103	15	measurement	measurement	NN
cord-014597-66vd2mdu	2103	16	of	of	IN
cord-014597-66vd2mdu	2103	17	the	the	DT
cord-014597-66vd2mdu	2103	18	lipids	lipid	NNS
cord-014597-66vd2mdu	2103	19	.	.	.
cord-014597-66vd2mdu	2104	1	Lipids	lipid	NNS
cord-014597-66vd2mdu	2104	2	were	be	VBD
cord-014597-66vd2mdu	2104	3	extracted	extract	VBN
cord-014597-66vd2mdu	2104	4	by	by	IN
cord-014597-66vd2mdu	2104	5	Matyash	Matyash	NNP
cord-014597-66vd2mdu	2104	6	method	method	NN
cord-014597-66vd2mdu	2104	7	,	,	,
cord-014597-66vd2mdu	2104	8	separated	separate	VBN
cord-014597-66vd2mdu	2104	9	by	by	IN
cord-014597-66vd2mdu	2104	10	HILIC	HILIC	NNP
cord-014597-66vd2mdu	2104	11	and	and	CC
cord-014597-66vd2mdu	2104	12	detected	detect	VBN
cord-014597-66vd2mdu	2104	13	by	by	IN
cord-014597-66vd2mdu	2104	14	tandem	tandem	JJ
cord-014597-66vd2mdu	2104	15	mass	mass	NN
cord-014597-66vd2mdu	2104	16	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	2104	17	.	.	.
cord-014597-66vd2mdu	2105	1	This	this	DT
cord-014597-66vd2mdu	2105	2	method	method	NN
cord-014597-66vd2mdu	2105	3	is	be	VBZ
cord-014597-66vd2mdu	2105	4	suitable	suitable	JJ
cord-014597-66vd2mdu	2105	5	for	for	IN
cord-014597-66vd2mdu	2105	6	both	both	DT
cord-014597-66vd2mdu	2105	7	in	in	IN
cord-014597-66vd2mdu	2105	8	process	process	NN
cord-014597-66vd2mdu	2105	9	sample	sample	NN
cord-014597-66vd2mdu	2105	10	lipid	lipid	NN
cord-014597-66vd2mdu	2105	11	analysis	analysis	NN
cord-014597-66vd2mdu	2105	12	providing	provide	VBG
cord-014597-66vd2mdu	2105	13	information	information	NN
cord-014597-66vd2mdu	2105	14	on	on	IN
cord-014597-66vd2mdu	2105	15	the	the	DT
cord-014597-66vd2mdu	2105	16	cell	cell	NN
cord-014597-66vd2mdu	2105	17	lipid	lipid	NN
cord-014597-66vd2mdu	2105	18	content	content	NN
cord-014597-66vd2mdu	2105	19	,	,	,
cord-014597-66vd2mdu	2105	20	and	and	CC
cord-014597-66vd2mdu	2105	21	for	for	IN
cord-014597-66vd2mdu	2105	22	harvest	harvest	NN
cord-014597-66vd2mdu	2105	23	samples	sample	NNS
cord-014597-66vd2mdu	2105	24	,	,	,
cord-014597-66vd2mdu	2105	25	enabling	enable	VBG
cord-014597-66vd2mdu	2105	26	to	to	TO
cord-014597-66vd2mdu	2105	27	follow	follow	VB
cord-014597-66vd2mdu	2105	28	the	the	DT
cord-014597-66vd2mdu	2105	29	lipid	lipid	NN
cord-014597-66vd2mdu	2105	30	release	release	NN
cord-014597-66vd2mdu	2105	31	during	during	IN
cord-014597-66vd2mdu	2105	32	the	the	DT
cord-014597-66vd2mdu	2105	33	different	different	JJ
cord-014597-66vd2mdu	2105	34	harvest	harvest	NN
cord-014597-66vd2mdu	2105	35	steps	step	NNS
cord-014597-66vd2mdu	2105	36	.	.	.
cord-014597-66vd2mdu	2106	1	This	this	DT
cord-014597-66vd2mdu	2106	2	non	non	JJ
cord-014597-66vd2mdu	2106	3	-	-	JJ
cord-014597-66vd2mdu	2106	4	targeted	targeted	JJ
cord-014597-66vd2mdu	2106	5	lipidomic	lipidomic	JJ
cord-014597-66vd2mdu	2106	6	quantitation	quantitation	NN
cord-014597-66vd2mdu	2106	7	method	method	NN
cord-014597-66vd2mdu	2106	8	will	will	MD
cord-014597-66vd2mdu	2106	9	enable	enable	VB
cord-014597-66vd2mdu	2106	10	us	-PRON-	PRP
cord-014597-66vd2mdu	2106	11	to	to	TO
cord-014597-66vd2mdu	2106	12	better	well	RBR
cord-014597-66vd2mdu	2106	13	control	control	VB
cord-014597-66vd2mdu	2106	14	lipid	lipid	NN
cord-014597-66vd2mdu	2106	15	synthesis	synthesis	NN
cord-014597-66vd2mdu	2106	16	during	during	IN
cord-014597-66vd2mdu	2106	17	biopharmaceutical	biopharmaceutical	JJ
cord-014597-66vd2mdu	2106	18	fed	fed	NNP
cord-014597-66vd2mdu	2106	19	batch	batch	NN
cord-014597-66vd2mdu	2106	20	production	production	NN
cord-014597-66vd2mdu	2106	21	through	through	IN
cord-014597-66vd2mdu	2106	22	clone	clone	NN
cord-014597-66vd2mdu	2106	23	selection	selection	NN
cord-014597-66vd2mdu	2106	24	,	,	,
cord-014597-66vd2mdu	2106	25	metabolomics	metabolomic	NNS
cord-014597-66vd2mdu	2106	26	studies	study	NNS
cord-014597-66vd2mdu	2106	27	and	and	CC
cord-014597-66vd2mdu	2106	28	harvest	harvest	VB
cord-014597-66vd2mdu	2106	29	development	development	NN
cord-014597-66vd2mdu	2106	30	.	.	.
cord-014597-66vd2mdu	2107	1	Background	Background	NNP
cord-014597-66vd2mdu	2107	2	Human	human	JJ
cord-014597-66vd2mdu	2107	3	mesenchymal	mesenchymal	JJ
cord-014597-66vd2mdu	2107	4	stem	stem	NN
cord-014597-66vd2mdu	2107	5	/	/	SYM
cord-014597-66vd2mdu	2107	6	stromal	stromal	NN
cord-014597-66vd2mdu	2107	7	cells	cell	NNS
cord-014597-66vd2mdu	2107	8	(	(	-LRB-
cord-014597-66vd2mdu	2107	9	hMSC	hmsc	NN
cord-014597-66vd2mdu	2107	10	)	)	-RRB-
cord-014597-66vd2mdu	2107	11	can	can	MD
cord-014597-66vd2mdu	2107	12	easily	easily	RB
cord-014597-66vd2mdu	2107	13	be	be	VB
cord-014597-66vd2mdu	2107	14	isolated	isolate	VBN
cord-014597-66vd2mdu	2107	15	from	from	IN
cord-014597-66vd2mdu	2107	16	e.g.	e.g.	JJ
cord-014597-66vd2mdu	2107	17	bone	bone	NN
cord-014597-66vd2mdu	2107	18	marrow	marrow	NN
cord-014597-66vd2mdu	2107	19	,	,	,
cord-014597-66vd2mdu	2107	20	fat	fat	JJ
cord-014597-66vd2mdu	2107	21	tissue	tissue	NN
cord-014597-66vd2mdu	2107	22	or	or	CC
cord-014597-66vd2mdu	2107	23	umbilical	umbilical	JJ
cord-014597-66vd2mdu	2107	24	cord	cord	NN
cord-014597-66vd2mdu	2107	25	blood	blood	NN
cord-014597-66vd2mdu	2107	26	and	and	CC
cord-014597-66vd2mdu	2107	27	are	be	VBP
cord-014597-66vd2mdu	2107	28	therefore	therefore	RB
cord-014597-66vd2mdu	2107	29	a	a	DT
cord-014597-66vd2mdu	2107	30	central	central	JJ
cord-014597-66vd2mdu	2107	31	player	player	NN
cord-014597-66vd2mdu	2107	32	in	in	IN
cord-014597-66vd2mdu	2107	33	regenerative	regenerative	JJ
cord-014597-66vd2mdu	2107	34	medicine	medicine	NN
cord-014597-66vd2mdu	2107	35	,	,	,
cord-014597-66vd2mdu	2107	36	gene	gene	NN
cord-014597-66vd2mdu	2107	37	therapy	therapy	NN
cord-014597-66vd2mdu	2107	38	and	and	CC
cord-014597-66vd2mdu	2107	39	cell	cell	NN
cord-014597-66vd2mdu	2107	40	therapy	therapy	NN
cord-014597-66vd2mdu	2107	41	[	[	-LRB-
cord-014597-66vd2mdu	2107	42	1	1	CD
cord-014597-66vd2mdu	2107	43	]	]	-RRB-
cord-014597-66vd2mdu	2107	44	[	[	-LRB-
cord-014597-66vd2mdu	2107	45	2	2	CD
cord-014597-66vd2mdu	2107	46	]	]	-RRB-
cord-014597-66vd2mdu	2107	47	[	[	-LRB-
cord-014597-66vd2mdu	2107	48	3	3	CD
cord-014597-66vd2mdu	2107	49	]	]	-RRB-
cord-014597-66vd2mdu	2107	50	.	.	.
cord-014597-66vd2mdu	2108	1	The	the	DT
cord-014597-66vd2mdu	2108	2	necessary	necessary	JJ
cord-014597-66vd2mdu	2108	3	gene	gene	NN
cord-014597-66vd2mdu	2108	4	shuttle	shuttle	NN
cord-014597-66vd2mdu	2108	5	is	be	VBZ
cord-014597-66vd2mdu	2108	6	mainly	mainly	RB
cord-014597-66vd2mdu	2108	7	provided	provide	VBN
cord-014597-66vd2mdu	2108	8	by	by	IN
cord-014597-66vd2mdu	2108	9	viruses	virus	NNS
cord-014597-66vd2mdu	2108	10	associated	associate	VBN
cord-014597-66vd2mdu	2108	11	with	with	IN
cord-014597-66vd2mdu	2108	12	diseases	disease	NNS
cord-014597-66vd2mdu	2108	13	,	,	,
cord-014597-66vd2mdu	2108	14	like	like	IN
cord-014597-66vd2mdu	2108	15	retrovirus	retrovirus	NN
cord-014597-66vd2mdu	2108	16	or	or	CC
cord-014597-66vd2mdu	2108	17	adenovirus	adenovirus	NN
cord-014597-66vd2mdu	2108	18	[	[	-LRB-
cord-014597-66vd2mdu	2108	19	4	4	CD
cord-014597-66vd2mdu	2108	20	]	]	-RRB-
cord-014597-66vd2mdu	2108	21	[	[	-LRB-
cord-014597-66vd2mdu	2108	22	5	5	CD
cord-014597-66vd2mdu	2108	23	]	]	-RRB-
cord-014597-66vd2mdu	2108	24	[	[	-LRB-
cord-014597-66vd2mdu	2108	25	6	6	CD
cord-014597-66vd2mdu	2108	26	]	]	-RRB-
cord-014597-66vd2mdu	2108	27	[	[	-LRB-
cord-014597-66vd2mdu	2108	28	7	7	CD
cord-014597-66vd2mdu	2108	29	]	]	-RRB-
cord-014597-66vd2mdu	2108	30	.	.	.
cord-014597-66vd2mdu	2109	1	These	these	DT
cord-014597-66vd2mdu	2109	2	possible	possible	JJ
cord-014597-66vd2mdu	2109	3	pathogen	pathogen	NN
cord-014597-66vd2mdu	2109	4	viruses	virus	NNS
cord-014597-66vd2mdu	2109	5	demand	demand	VBP
cord-014597-66vd2mdu	2109	6	for	for	IN
cord-014597-66vd2mdu	2109	7	high	high	JJ
cord-014597-66vd2mdu	2109	8	safety	safety	NN
cord-014597-66vd2mdu	2109	9	standards	standard	NNS
cord-014597-66vd2mdu	2109	10	.	.	.
cord-014597-66vd2mdu	2110	1	Also	also	RB
cord-014597-66vd2mdu	2110	2	,	,	,
cord-014597-66vd2mdu	2110	3	they	-PRON-	PRP
cord-014597-66vd2mdu	2110	4	are	be	VBP
cord-014597-66vd2mdu	2110	5	prone	prone	JJ
cord-014597-66vd2mdu	2110	6	to	to	IN
cord-014597-66vd2mdu	2110	7	genomic	genomic	JJ
cord-014597-66vd2mdu	2110	8	alterations	alteration	NNS
cord-014597-66vd2mdu	2110	9	and	and	CC
cord-014597-66vd2mdu	2110	10	there	there	EX
cord-014597-66vd2mdu	2110	11	is	be	VBZ
cord-014597-66vd2mdu	2110	12	the	the	DT
cord-014597-66vd2mdu	2110	13	possibility	possibility	NN
cord-014597-66vd2mdu	2110	14	of	of	IN
cord-014597-66vd2mdu	2110	15	virus	virus	NN
cord-014597-66vd2mdu	2110	16	inactivation	inactivation	NN
cord-014597-66vd2mdu	2110	17	,	,	,
cord-014597-66vd2mdu	2110	18	triggered	trigger	VBD
cord-014597-66vd2mdu	2110	19	due	due	IN
cord-014597-66vd2mdu	2110	20	to	to	IN
cord-014597-66vd2mdu	2110	21	pre	pre	VB
cord-014597-66vd2mdu	2110	22	-	-	JJ
cord-014597-66vd2mdu	2110	23	existing	existing	JJ
cord-014597-66vd2mdu	2110	24	immunity	immunity	NN
cord-014597-66vd2mdu	2110	25	in	in	IN
cord-014597-66vd2mdu	2110	26	the	the	DT
cord-014597-66vd2mdu	2110	27	patient	patient	NN
cord-014597-66vd2mdu	2110	28	[	[	-LRB-
cord-014597-66vd2mdu	2110	29	8	8	CD
cord-014597-66vd2mdu	2110	30	]	]	-RRB-
cord-014597-66vd2mdu	2110	31	[	[	-LRB-
cord-014597-66vd2mdu	2110	32	9	9	CD
cord-014597-66vd2mdu	2110	33	]	]	-RRB-
cord-014597-66vd2mdu	2110	34	[	[	-LRB-
cord-014597-66vd2mdu	2110	35	10	10	CD
cord-014597-66vd2mdu	2110	36	]	]	-RRB-
cord-014597-66vd2mdu	2110	37	.	.	.
cord-014597-66vd2mdu	2111	1	In	in	IN
cord-014597-66vd2mdu	2111	2	this	this	DT
cord-014597-66vd2mdu	2111	3	context	context	NN
cord-014597-66vd2mdu	2111	4	,	,	,
cord-014597-66vd2mdu	2111	5	the	the	DT
cord-014597-66vd2mdu	2111	6	Autographa	Autographa	NNP
cord-014597-66vd2mdu	2111	7	californica	californica	NN
cord-014597-66vd2mdu	2111	8	multicapsid	multicapsid	NNP
cord-014597-66vd2mdu	2111	9	nucleopolyhedrovirus	nucleopolyhedrovirus	NNP
cord-014597-66vd2mdu	2111	10	(	(	-LRB-
cord-014597-66vd2mdu	2111	11	AcMNPV	AcMNPV	NNP
cord-014597-66vd2mdu	2111	12	)	)	-RRB-
cord-014597-66vd2mdu	2111	13	is	be	VBZ
cord-014597-66vd2mdu	2111	14	a	a	DT
cord-014597-66vd2mdu	2111	15	safe	safe	JJ
cord-014597-66vd2mdu	2111	16	alternative	alternative	NN
cord-014597-66vd2mdu	2111	17	.	.	.
cord-014597-66vd2mdu	2112	1	The	the	DT
cord-014597-66vd2mdu	2112	2	virus	virus	NN
cord-014597-66vd2mdu	2112	3	replication	replication	NN
cord-014597-66vd2mdu	2112	4	is	be	VBZ
cord-014597-66vd2mdu	2112	5	hostspecific	hostspecific	JJ
cord-014597-66vd2mdu	2112	6	for	for	IN
cord-014597-66vd2mdu	2112	7	insects	insect	NNS
cord-014597-66vd2mdu	2112	8	[	[	-LRB-
cord-014597-66vd2mdu	2112	9	11	11	CD
cord-014597-66vd2mdu	2112	10	]	]	-RRB-
cord-014597-66vd2mdu	2112	11	,	,	,
cord-014597-66vd2mdu	2112	12	but	but	CC
cord-014597-66vd2mdu	2112	13	it	-PRON-	PRP
cord-014597-66vd2mdu	2112	14	is	be	VBZ
cord-014597-66vd2mdu	2112	15	known	know	VBN
cord-014597-66vd2mdu	2112	16	since	since	IN
cord-014597-66vd2mdu	2112	17	the	the	DT
cord-014597-66vd2mdu	2112	18	mid-90s	mid-90	NNS
cord-014597-66vd2mdu	2112	19	,	,	,
cord-014597-66vd2mdu	2112	20	that	that	IN
cord-014597-66vd2mdu	2112	21	a	a	DT
cord-014597-66vd2mdu	2112	22	temporary	temporary	JJ
cord-014597-66vd2mdu	2112	23	transduction	transduction	NN
cord-014597-66vd2mdu	2112	24	of	of	IN
cord-014597-66vd2mdu	2112	25	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2112	26	cells	cell	NNS
cord-014597-66vd2mdu	2112	27	is	be	VBZ
cord-014597-66vd2mdu	2112	28	possible	possible	JJ
cord-014597-66vd2mdu	2112	29	[	[	-LRB-
cord-014597-66vd2mdu	2112	30	12	12	CD
cord-014597-66vd2mdu	2112	31	]	]	-RRB-
cord-014597-66vd2mdu	2112	32	.	.	.
cord-014597-66vd2mdu	2113	1	Some	some	DT
cord-014597-66vd2mdu	2113	2	modifications	modification	NNS
cord-014597-66vd2mdu	2113	3	of	of	IN
cord-014597-66vd2mdu	2113	4	the	the	DT
cord-014597-66vd2mdu	2113	5	virus	virus	NN
cord-014597-66vd2mdu	2113	6	increased	increase	VBD
cord-014597-66vd2mdu	2113	7	the	the	DT
cord-014597-66vd2mdu	2113	8	applicability	applicability	NN
cord-014597-66vd2mdu	2113	9	in	in	IN
cord-014597-66vd2mdu	2113	10	stem	stem	NN
cord-014597-66vd2mdu	2113	11	cells	cell	NNS
cord-014597-66vd2mdu	2113	12	.	.	.
cord-014597-66vd2mdu	2114	1	Pseudotyping	pseudotype	VBG
cord-014597-66vd2mdu	2114	2	the	the	DT
cord-014597-66vd2mdu	2114	3	virus	virus	NN
cord-014597-66vd2mdu	2114	4	with	with	IN
cord-014597-66vd2mdu	2114	5	the	the	DT
cord-014597-66vd2mdu	2114	6	vesicular	vesicular	JJ
cord-014597-66vd2mdu	2114	7	stomatitis	stomatitis	NN
cord-014597-66vd2mdu	2114	8	glycoprotein	glycoprotein	NN
cord-014597-66vd2mdu	2114	9	(	(	-LRB-
cord-014597-66vd2mdu	2114	10	VSV	VSV	NNP
cord-014597-66vd2mdu	2114	11	-	-	HYPH
cord-014597-66vd2mdu	2114	12	G	G	NNP
cord-014597-66vd2mdu	2114	13	)	)	-RRB-
cord-014597-66vd2mdu	2114	14	led	lead	VBD
cord-014597-66vd2mdu	2114	15	to	to	IN
cord-014597-66vd2mdu	2114	16	an	an	DT
cord-014597-66vd2mdu	2114	17	expansion	expansion	NN
cord-014597-66vd2mdu	2114	18	of	of	IN
cord-014597-66vd2mdu	2114	19	the	the	DT
cord-014597-66vd2mdu	2114	20	transducable	transducable	JJ
cord-014597-66vd2mdu	2114	21	cell	cell	NN
cord-014597-66vd2mdu	2114	22	[	[	-LRB-
cord-014597-66vd2mdu	2114	23	13	13	CD
cord-014597-66vd2mdu	2114	24	,	,	,
cord-014597-66vd2mdu	2114	25	14	14	CD
cord-014597-66vd2mdu	2114	26	]	]	-RRB-
cord-014597-66vd2mdu	2114	27	and	and	CC
cord-014597-66vd2mdu	2114	28	the	the	DT
cord-014597-66vd2mdu	2114	29	integration	integration	NN
cord-014597-66vd2mdu	2114	30	of	of	IN
cord-014597-66vd2mdu	2114	31	the	the	DT
cord-014597-66vd2mdu	2114	32	woodchuck	woodchuck	NNP
cord-014597-66vd2mdu	2114	33	hepatitis	hepatitis	NNP
cord-014597-66vd2mdu	2114	34	virus	virus	NN
cord-014597-66vd2mdu	2114	35	post	post	JJ
cord-014597-66vd2mdu	2114	36	-	-	JJ
cord-014597-66vd2mdu	2114	37	transcriptional	transcriptional	JJ
cord-014597-66vd2mdu	2114	38	regulatory	regulatory	JJ
cord-014597-66vd2mdu	2114	39	element	element	NN
cord-014597-66vd2mdu	2114	40	(	(	-LRB-
cord-014597-66vd2mdu	2114	41	WPRE	WPRE	NNP
cord-014597-66vd2mdu	2114	42	)	)	-RRB-
cord-014597-66vd2mdu	2114	43	prolonged	prolong	VBD
cord-014597-66vd2mdu	2114	44	the	the	DT
cord-014597-66vd2mdu	2114	45	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2114	46	protein	protein	NN
cord-014597-66vd2mdu	2114	47	expression	expression	NN
cord-014597-66vd2mdu	2114	48	[	[	-LRB-
cord-014597-66vd2mdu	2114	49	15	15	CD
cord-014597-66vd2mdu	2114	50	,	,	,
cord-014597-66vd2mdu	2114	51	16	16	CD
cord-014597-66vd2mdu	2114	52	]	]	-RRB-
cord-014597-66vd2mdu	2114	53	.	.	.
cord-014597-66vd2mdu	2115	1	For	for	IN
cord-014597-66vd2mdu	2115	2	achieving	achieve	VBG
cord-014597-66vd2mdu	2115	3	a	a	DT
cord-014597-66vd2mdu	2115	4	baculovirus	baculovirus	NN
cord-014597-66vd2mdu	2115	5	-	-	HYPH
cord-014597-66vd2mdu	2115	6	induced	induce	VBN
cord-014597-66vd2mdu	2115	7	differentiation	differentiation	NN
cord-014597-66vd2mdu	2115	8	of	of	IN
cord-014597-66vd2mdu	2115	9	hMSCs	hMSCs	NNP
cord-014597-66vd2mdu	2115	10	,	,	,
cord-014597-66vd2mdu	2115	11	the	the	DT
cord-014597-66vd2mdu	2115	12	promotor	promotor	NN
cord-014597-66vd2mdu	2115	13	and	and	CC
cord-014597-66vd2mdu	2115	14	the	the	DT
cord-014597-66vd2mdu	2115	15	expression	expression	NN
cord-014597-66vd2mdu	2115	16	strength	strength	NN
cord-014597-66vd2mdu	2115	17	of	of	IN
cord-014597-66vd2mdu	2115	18	the	the	DT
cord-014597-66vd2mdu	2115	19	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2115	20	protein	protein	NN
cord-014597-66vd2mdu	2115	21	are	be	VBP
cord-014597-66vd2mdu	2115	22	crucial	crucial	JJ
cord-014597-66vd2mdu	2115	23	factors	factor	NNS
cord-014597-66vd2mdu	2115	24	.	.	.
cord-014597-66vd2mdu	2116	1	Still	still	RB
cord-014597-66vd2mdu	2116	2	,	,	,
cord-014597-66vd2mdu	2116	3	there	there	EX
cord-014597-66vd2mdu	2116	4	are	be	VBP
cord-014597-66vd2mdu	2116	5	still	still	RB
cord-014597-66vd2mdu	2116	6	few	few	JJ
cord-014597-66vd2mdu	2116	7	comparative	comparative	JJ
cord-014597-66vd2mdu	2116	8	promotor	promotor	NN
cord-014597-66vd2mdu	2116	9	studies	study	NNS
cord-014597-66vd2mdu	2116	10	[	[	-LRB-
cord-014597-66vd2mdu	2116	11	17	17	CD
cord-014597-66vd2mdu	2116	12	,	,	,
cord-014597-66vd2mdu	2116	13	18	18	CD
cord-014597-66vd2mdu	2116	14	]	]	-RRB-
cord-014597-66vd2mdu	2116	15	.	.	.
cord-014597-66vd2mdu	2117	1	However	however	RB
cord-014597-66vd2mdu	2117	2	,	,	,
cord-014597-66vd2mdu	2117	3	a	a	DT
cord-014597-66vd2mdu	2117	4	successful	successful	JJ
cord-014597-66vd2mdu	2117	5	virus	virus	NN
cord-014597-66vd2mdu	2117	6	uptake	uptake	NN
cord-014597-66vd2mdu	2117	7	is	be	VBZ
cord-014597-66vd2mdu	2117	8	the	the	DT
cord-014597-66vd2mdu	2117	9	prerequisite	prerequisite	NN
cord-014597-66vd2mdu	2117	10	for	for	IN
cord-014597-66vd2mdu	2117	11	a	a	DT
cord-014597-66vd2mdu	2117	12	successful	successful	JJ
cord-014597-66vd2mdu	2117	13	protein	protein	NN
cord-014597-66vd2mdu	2117	14	expression	expression	NN
cord-014597-66vd2mdu	2117	15	.	.	.
cord-014597-66vd2mdu	2118	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2118	2	therefore	therefore	RB
cord-014597-66vd2mdu	2118	3	investigated	investigate	VBD
cord-014597-66vd2mdu	2118	4	factors	factor	NNS
cord-014597-66vd2mdu	2118	5	significantly	significantly	RB
cord-014597-66vd2mdu	2118	6	influencing	influence	VBG
cord-014597-66vd2mdu	2118	7	the	the	DT
cord-014597-66vd2mdu	2118	8	transduction	transduction	NN
cord-014597-66vd2mdu	2118	9	process	process	NN
cord-014597-66vd2mdu	2118	10	by	by	IN
cord-014597-66vd2mdu	2118	11	applying	apply	VBG
cord-014597-66vd2mdu	2118	12	design	design	NN
cord-014597-66vd2mdu	2118	13	of	of	IN
cord-014597-66vd2mdu	2118	14	experiments	experiment	NNS
cord-014597-66vd2mdu	2118	15	(	(	-LRB-
cord-014597-66vd2mdu	2118	16	s.	s.	NNP
cord-014597-66vd2mdu	2118	17	Fig	Fig	NNP
cord-014597-66vd2mdu	2118	18	.	.	.
cord-014597-66vd2mdu	2118	19	1a	1a	CD
cord-014597-66vd2mdu	2118	20	)	)	-RRB-
cord-014597-66vd2mdu	2118	21	.	.	.
cord-014597-66vd2mdu	2119	1	The	the	DT
cord-014597-66vd2mdu	2119	2	experimental	experimental	JJ
cord-014597-66vd2mdu	2119	3	design	design	NN
cord-014597-66vd2mdu	2119	4	comprises	comprise	VBZ
cord-014597-66vd2mdu	2119	5	a	a	DT
cord-014597-66vd2mdu	2119	6	two	two	CD
cord-014597-66vd2mdu	2119	7	level	level	NN
cord-014597-66vd2mdu	2119	8	factorial	factorial	NN
cord-014597-66vd2mdu	2119	9	screening	screening	NN
cord-014597-66vd2mdu	2119	10	,	,	,
cord-014597-66vd2mdu	2119	11	set	set	NN
cord-014597-66vd2mdu	2119	12	-	-	HYPH
cord-014597-66vd2mdu	2119	13	up	up	NN
cord-014597-66vd2mdu	2119	14	using	use	VBG
cord-014597-66vd2mdu	2119	15	Design	Design	NNP
cord-014597-66vd2mdu	2119	16	Expert	expert	NN
cord-014597-66vd2mdu	2119	17	V9.For	V9.For	NNP
cord-014597-66vd2mdu	2120	1	the	the	DT
cord-014597-66vd2mdu	2120	2	transduction	transduction	NN
cord-014597-66vd2mdu	2120	3	60,000	60,000	CD
cord-014597-66vd2mdu	2120	4	c	c	NN
cord-014597-66vd2mdu	2120	5	/	/	SYM
cord-014597-66vd2mdu	2120	6	cm	cm	NNP
cord-014597-66vd2mdu	2120	7	2	2	CD
cord-014597-66vd2mdu	2120	8	were	be	VBD
cord-014597-66vd2mdu	2120	9	seeded	seed	VBN
cord-014597-66vd2mdu	2120	10	in	in	IN
cord-014597-66vd2mdu	2120	11	24-well	24-well	CD
cord-014597-66vd2mdu	2120	12	plates	plate	NNS
cord-014597-66vd2mdu	2120	13	with	with	IN
cord-014597-66vd2mdu	2120	14	DMEM	DMEM	NNP
cord-014597-66vd2mdu	2120	15	+	+	CC
cord-014597-66vd2mdu	2120	16	10	10	CD
cord-014597-66vd2mdu	2120	17	%	%	NN
cord-014597-66vd2mdu	2120	18	FCS	FCS	NNP
cord-014597-66vd2mdu	2120	19	and	and	CC
cord-014597-66vd2mdu	2120	20	incubated	incubate	VBN
cord-014597-66vd2mdu	2120	21	overnight	overnight	RB
cord-014597-66vd2mdu	2120	22	at	at	IN
cord-014597-66vd2mdu	2120	23	37	37	CD
cord-014597-66vd2mdu	2120	24	°	°	NN
cord-014597-66vd2mdu	2120	25	C	c	NN
cord-014597-66vd2mdu	2120	26	,	,	,
cord-014597-66vd2mdu	2120	27	8	8	CD
cord-014597-66vd2mdu	2120	28	%	%	NN
cord-014597-66vd2mdu	2120	29	CO	co	NN
cord-014597-66vd2mdu	2120	30	2	2	CD
cord-014597-66vd2mdu	2120	31	and	and	CC
cord-014597-66vd2mdu	2120	32	humidified	humidified	JJ
cord-014597-66vd2mdu	2120	33	atmosphere	atmosphere	NN
cord-014597-66vd2mdu	2120	34	.	.	.
cord-014597-66vd2mdu	2121	1	The	the	DT
cord-014597-66vd2mdu	2121	2	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2121	3	baculovirus	baculovirus	NN
cord-014597-66vd2mdu	2121	4	using	use	VBG
cord-014597-66vd2mdu	2121	5	an	an	DT
cord-014597-66vd2mdu	2121	6	integrated	integrate	VBN
cord-014597-66vd2mdu	2121	7	EF1α	EF1α	NNP
cord-014597-66vd2mdu	2121	8	promoter	promoter	NN
cord-014597-66vd2mdu	2121	9	to	to	TO
cord-014597-66vd2mdu	2121	10	control	control	VB
cord-014597-66vd2mdu	2121	11	GFP	GFP	NNP
cord-014597-66vd2mdu	2121	12	expression	expression	NN
cord-014597-66vd2mdu	2121	13	,	,	,
cord-014597-66vd2mdu	2121	14	described	describe	VBN
cord-014597-66vd2mdu	2121	15	elsewhere	elsewhere	RB
cord-014597-66vd2mdu	2121	16	[	[	-LRB-
cord-014597-66vd2mdu	2121	17	18	18	CD
cord-014597-66vd2mdu	2121	18	]	]	-RRB-
cord-014597-66vd2mdu	2121	19	,	,	,
cord-014597-66vd2mdu	2121	20	was	be	VBD
cord-014597-66vd2mdu	2121	21	diluted	dilute	VBN
cord-014597-66vd2mdu	2121	22	to	to	IN
cord-014597-66vd2mdu	2121	23	the	the	DT
cord-014597-66vd2mdu	2121	24	respective	respective	JJ
cord-014597-66vd2mdu	2121	25	concentrations	concentration	NNS
cord-014597-66vd2mdu	2121	26	in	in	IN
cord-014597-66vd2mdu	2121	27	the	the	DT
cord-014597-66vd2mdu	2121	28	different	different	JJ
cord-014597-66vd2mdu	2121	29	surrounding	surround	VBG
cord-014597-66vd2mdu	2121	30	fluids	fluid	NNS
cord-014597-66vd2mdu	2121	31	.	.	.
cord-014597-66vd2mdu	2122	1	After	after	IN
cord-014597-66vd2mdu	2122	2	discarding	discard	VBG
cord-014597-66vd2mdu	2122	3	the	the	DT
cord-014597-66vd2mdu	2122	4	cultivation	cultivation	NN
cord-014597-66vd2mdu	2122	5	medium	medium	NN
cord-014597-66vd2mdu	2122	6	of	of	IN
cord-014597-66vd2mdu	2122	7	the	the	DT
cord-014597-66vd2mdu	2122	8	hMSC	hmsc	NN
cord-014597-66vd2mdu	2122	9	-	-	HYPH
cord-014597-66vd2mdu	2122	10	TERT	TERT	NNP
cord-014597-66vd2mdu	2122	11	,	,	,
cord-014597-66vd2mdu	2122	12	1	1	CD
cord-014597-66vd2mdu	2122	13	mL	ml	NN
cord-014597-66vd2mdu	2122	14	of	of	IN
cord-014597-66vd2mdu	2122	15	virus	virus	NN
cord-014597-66vd2mdu	2122	16	containing	contain	VBG
cord-014597-66vd2mdu	2122	17	solution	solution	NN
cord-014597-66vd2mdu	2122	18	was	be	VBD
cord-014597-66vd2mdu	2122	19	added	add	VBN
cord-014597-66vd2mdu	2122	20	to	to	IN
cord-014597-66vd2mdu	2122	21	the	the	DT
cord-014597-66vd2mdu	2122	22	cells	cell	NNS
cord-014597-66vd2mdu	2122	23	.	.	.
cord-014597-66vd2mdu	2123	1	The	the	DT
cord-014597-66vd2mdu	2123	2	following	follow	VBG
cord-014597-66vd2mdu	2123	3	incubation	incubation	NN
cord-014597-66vd2mdu	2123	4	was	be	VBD
cord-014597-66vd2mdu	2123	5	varied	varied	JJ
cord-014597-66vd2mdu	2123	6	in	in	IN
cord-014597-66vd2mdu	2123	7	duration	duration	NN
cord-014597-66vd2mdu	2123	8	before	before	IN
cord-014597-66vd2mdu	2123	9	replacing	replace	VBG
cord-014597-66vd2mdu	2123	10	the	the	DT
cord-014597-66vd2mdu	2123	11	virus	virus	NN
cord-014597-66vd2mdu	2123	12	solution	solution	NN
cord-014597-66vd2mdu	2123	13	with	with	IN
cord-014597-66vd2mdu	2123	14	growth	growth	NN
cord-014597-66vd2mdu	2123	15	medium	medium	NN
cord-014597-66vd2mdu	2123	16	and	and	CC
cord-014597-66vd2mdu	2123	17	an	an	DT
cord-014597-66vd2mdu	2123	18	incubation	incubation	NN
cord-014597-66vd2mdu	2123	19	overnight	overnight	RB
cord-014597-66vd2mdu	2123	20	.	.	.
cord-014597-66vd2mdu	2124	1	24	24	CD
cord-014597-66vd2mdu	2124	2	h	h	NN
cord-014597-66vd2mdu	2124	3	post	post	NN
cord-014597-66vd2mdu	2124	4	transduction	transduction	NN
cord-014597-66vd2mdu	2124	5	(	(	-LRB-
cord-014597-66vd2mdu	2124	6	hpt	hpt	NNP
cord-014597-66vd2mdu	2124	7	)	)	-RRB-
cord-014597-66vd2mdu	2124	8	the	the	DT
cord-014597-66vd2mdu	2124	9	cells	cell	NNS
cord-014597-66vd2mdu	2124	10	were	be	VBD
cord-014597-66vd2mdu	2124	11	washed	wash	VBN
cord-014597-66vd2mdu	2124	12	with	with	IN
cord-014597-66vd2mdu	2124	13	PBS	PBS	NNP
cord-014597-66vd2mdu	2124	14	,	,	,
cord-014597-66vd2mdu	2124	15	trypsinized	trypsinize	VBN
cord-014597-66vd2mdu	2124	16	with	with	IN
cord-014597-66vd2mdu	2124	17	100	100	CD
cord-014597-66vd2mdu	2124	18	μL	μL	NNP
cord-014597-66vd2mdu	2124	19	Trypsin	Trypsin	NNP
cord-014597-66vd2mdu	2124	20	/	/	SYM
cord-014597-66vd2mdu	2124	21	EDTA	EDTA	NNP
cord-014597-66vd2mdu	2124	22	and	and	CC
cord-014597-66vd2mdu	2124	23	incubated	incubate	VBN
cord-014597-66vd2mdu	2124	24	for	for	IN
cord-014597-66vd2mdu	2124	25	5	5	CD
cord-014597-66vd2mdu	2124	26	min	min	NN
cord-014597-66vd2mdu	2124	27	at	at	IN
cord-014597-66vd2mdu	2124	28	37	37	CD
cord-014597-66vd2mdu	2124	29	°	°	NN
cord-014597-66vd2mdu	2124	30	C	c	NN
cord-014597-66vd2mdu	2124	31	.	.	.
cord-014597-66vd2mdu	2125	1	Trypsination	trypsination	NN
cord-014597-66vd2mdu	2125	2	was	be	VBD
cord-014597-66vd2mdu	2125	3	then	then	RB
cord-014597-66vd2mdu	2125	4	stopped	stop	VBN
cord-014597-66vd2mdu	2125	5	applying	apply	VBG
cord-014597-66vd2mdu	2125	6	100	100	CD
cord-014597-66vd2mdu	2125	7	μL	μl	CD
cord-014597-66vd2mdu	2125	8	soybean	soybean	NN
cord-014597-66vd2mdu	2125	9	trypsin	trypsin	NN
cord-014597-66vd2mdu	2125	10	inhibitor	inhibitor	NN
cord-014597-66vd2mdu	2125	11	and	and	CC
cord-014597-66vd2mdu	2125	12	the	the	DT
cord-014597-66vd2mdu	2125	13	cells	cell	NNS
cord-014597-66vd2mdu	2125	14	were	be	VBD
cord-014597-66vd2mdu	2125	15	analyzed	analyze	VBN
cord-014597-66vd2mdu	2125	16	using	use	VBG
cord-014597-66vd2mdu	2125	17	flow	flow	NN
cord-014597-66vd2mdu	2125	18	cytometry	cytometry	NN
cord-014597-66vd2mdu	2125	19	.	.	.
cord-014597-66vd2mdu	2126	1	As	as	IN
cord-014597-66vd2mdu	2126	2	shown	show	VBN
cord-014597-66vd2mdu	2126	3	in	in	IN
cord-014597-66vd2mdu	2126	4	Fig	Fig	NNP
cord-014597-66vd2mdu	2126	5	.	.	.
cord-014597-66vd2mdu	2127	1	1a	1a	LS
cord-014597-66vd2mdu	2127	2	,	,	,
cord-014597-66vd2mdu	2127	3	the	the	DT
cord-014597-66vd2mdu	2127	4	virus	virus	NN
cord-014597-66vd2mdu	2127	5	concentration	concentration	NN
cord-014597-66vd2mdu	2127	6	and	and	CC
cord-014597-66vd2mdu	2127	7	incubation	incubation	NN
cord-014597-66vd2mdu	2127	8	time	time	NN
cord-014597-66vd2mdu	2127	9	exert	exert	VB
cord-014597-66vd2mdu	2127	10	the	the	DT
cord-014597-66vd2mdu	2127	11	highest	high	JJS
cord-014597-66vd2mdu	2127	12	influence	influence	NN
cord-014597-66vd2mdu	2127	13	on	on	IN
cord-014597-66vd2mdu	2127	14	the	the	DT
cord-014597-66vd2mdu	2127	15	transduction	transduction	NN
cord-014597-66vd2mdu	2127	16	efficiency	efficiency	NN
cord-014597-66vd2mdu	2127	17	.	.	.
cord-014597-66vd2mdu	2128	1	Obviously	obviously	RB
cord-014597-66vd2mdu	2128	2	,	,	,
cord-014597-66vd2mdu	2128	3	a	a	DT
cord-014597-66vd2mdu	2128	4	higher	high	JJR
cord-014597-66vd2mdu	2128	5	concentration	concentration	NN
cord-014597-66vd2mdu	2128	6	of	of	IN
cord-014597-66vd2mdu	2128	7	viral	viral	JJ
cord-014597-66vd2mdu	2128	8	particles	particle	NNS
cord-014597-66vd2mdu	2128	9	and	and	CC
cord-014597-66vd2mdu	2128	10	longer	long	JJR
cord-014597-66vd2mdu	2128	11	incubation	incubation	NN
cord-014597-66vd2mdu	2128	12	of	of	IN
cord-014597-66vd2mdu	2128	13	cells	cell	NNS
cord-014597-66vd2mdu	2128	14	with	with	IN
cord-014597-66vd2mdu	2128	15	virus	virus	NN
cord-014597-66vd2mdu	2128	16	increases	increase	VBZ
cord-014597-66vd2mdu	2128	17	the	the	DT
cord-014597-66vd2mdu	2128	18	probability	probability	NN
cord-014597-66vd2mdu	2128	19	for	for	IN
cord-014597-66vd2mdu	2128	20	hits	hit	NNS
cord-014597-66vd2mdu	2128	21	between	between	IN
cord-014597-66vd2mdu	2128	22	cells	cell	NNS
cord-014597-66vd2mdu	2128	23	and	and	CC
cord-014597-66vd2mdu	2128	24	virus	virus	NN
cord-014597-66vd2mdu	2128	25	particles	particle	NNS
cord-014597-66vd2mdu	2128	26	.	.	.
cord-014597-66vd2mdu	2129	1	Additionally	additionally	RB
cord-014597-66vd2mdu	2129	2	,	,	,
cord-014597-66vd2mdu	2129	3	the	the	DT
cord-014597-66vd2mdu	2129	4	surrounding	surround	VBG
cord-014597-66vd2mdu	2129	5	fluid	fluid	NN
cord-014597-66vd2mdu	2129	6	can	can	MD
cord-014597-66vd2mdu	2129	7	have	have	VB
cord-014597-66vd2mdu	2129	8	a	a	DT
cord-014597-66vd2mdu	2129	9	negative	negative	JJ
cord-014597-66vd2mdu	2129	10	impact	impact	NN
cord-014597-66vd2mdu	2129	11	on	on	IN
cord-014597-66vd2mdu	2129	12	the	the	DT
cord-014597-66vd2mdu	2129	13	transduction	transduction	NN
cord-014597-66vd2mdu	2129	14	.	.	.
cord-014597-66vd2mdu	2130	1	This	this	DT
cord-014597-66vd2mdu	2130	2	is	be	VBZ
cord-014597-66vd2mdu	2130	3	due	due	JJ
cord-014597-66vd2mdu	2130	4	to	to	IN
cord-014597-66vd2mdu	2130	5	the	the	DT
cord-014597-66vd2mdu	2130	6	interaction	interaction	NN
cord-014597-66vd2mdu	2130	7	of	of	IN
cord-014597-66vd2mdu	2130	8	medium	medium	JJ
cord-014597-66vd2mdu	2130	9	components	component	NNS
cord-014597-66vd2mdu	2130	10	with	with	IN
cord-014597-66vd2mdu	2130	11	the	the	DT
cord-014597-66vd2mdu	2130	12	baculovirus	baculovirus	NN
cord-014597-66vd2mdu	2130	13	.	.	.
cord-014597-66vd2mdu	2131	1	Therefore	therefore	RB
cord-014597-66vd2mdu	2131	2	,	,	,
cord-014597-66vd2mdu	2131	3	PBS	PBS	NNP
cord-014597-66vd2mdu	2131	4	containing	contain	VBG
cord-014597-66vd2mdu	2131	5	Ca	Ca	NNP
cord-014597-66vd2mdu	2131	6	2	2	CD
cord-014597-66vd2mdu	2131	7	+	+	SYM
cord-014597-66vd2mdu	2131	8	&	&	CC
cord-014597-66vd2mdu	2131	9	Mg	Mg	NNP
cord-014597-66vd2mdu	2131	10	2	2	CD
cord-014597-66vd2mdu	2132	1	+	+	UH
cord-014597-66vd2mdu	2132	2	is	be	VBZ
cord-014597-66vd2mdu	2132	3	recommended	recommend	VBN
cord-014597-66vd2mdu	2132	4	as	as	IN
cord-014597-66vd2mdu	2132	5	surrounding	surround	VBG
cord-014597-66vd2mdu	2132	6	fluid	fluid	NN
cord-014597-66vd2mdu	2132	7	for	for	IN
cord-014597-66vd2mdu	2132	8	transduction	transduction	NN
cord-014597-66vd2mdu	2132	9	experiments	experiment	NNS
cord-014597-66vd2mdu	2132	10	.	.	.
cord-014597-66vd2mdu	2133	1	In	in	IN
cord-014597-66vd2mdu	2133	2	Fig	Fig	NNP
cord-014597-66vd2mdu	2133	3	.	.	.
cord-014597-66vd2mdu	2134	1	1B	1B	NNP
cord-014597-66vd2mdu	2134	2	,	,	,
cord-014597-66vd2mdu	2134	3	the	the	DT
cord-014597-66vd2mdu	2134	4	transduction	transduction	NN
cord-014597-66vd2mdu	2134	5	conditions	condition	NNS
cord-014597-66vd2mdu	2134	6	resulting	result	VBG
cord-014597-66vd2mdu	2134	7	in	in	IN
cord-014597-66vd2mdu	2134	8	the	the	DT
cord-014597-66vd2mdu	2134	9	highest	high	JJS
cord-014597-66vd2mdu	2134	10	percentage	percentage	NN
cord-014597-66vd2mdu	2134	11	of	of	IN
cord-014597-66vd2mdu	2134	12	GFP+	GFP+	NNP
cord-014597-66vd2mdu	2134	13	cells	cell	NNS
cord-014597-66vd2mdu	2134	14	are	be	VBP
cord-014597-66vd2mdu	2134	15	displayed	display	VBN
cord-014597-66vd2mdu	2134	16	:	:	:
cord-014597-66vd2mdu	2134	17	150	150	CD
cord-014597-66vd2mdu	2134	18	virus	virus	NN
cord-014597-66vd2mdu	2134	19	particles	particle	NNS
cord-014597-66vd2mdu	2134	20	per	per	IN
cord-014597-66vd2mdu	2134	21	cell	cell	NN
cord-014597-66vd2mdu	2134	22	(	(	-LRB-
cord-014597-66vd2mdu	2134	23	PPC	PPC	NNP
cord-014597-66vd2mdu	2134	24	)	)	-RRB-
cord-014597-66vd2mdu	2134	25	and	and	CC
cord-014597-66vd2mdu	2134	26	an	an	DT
cord-014597-66vd2mdu	2134	27	incubation	incubation	NN
cord-014597-66vd2mdu	2134	28	time	time	NN
cord-014597-66vd2mdu	2134	29	of	of	IN
cord-014597-66vd2mdu	2134	30	5	5	CD
cord-014597-66vd2mdu	2134	31	h	h	NN
cord-014597-66vd2mdu	2134	32	with	with	IN
cord-014597-66vd2mdu	2134	33	hMSC	hmsc	NN
cord-014597-66vd2mdu	2134	34	-	-	HYPH
cord-014597-66vd2mdu	2134	35	TERT	tert	NN
cord-014597-66vd2mdu	2134	36	.	.	.
cord-014597-66vd2mdu	2135	1	The	the	DT
cord-014597-66vd2mdu	2135	2	experiments	experiment	NNS
cord-014597-66vd2mdu	2135	3	show	show	VBP
cord-014597-66vd2mdu	2135	4	,	,	,
cord-014597-66vd2mdu	2135	5	that	that	IN
cord-014597-66vd2mdu	2135	6	especially	especially	RB
cord-014597-66vd2mdu	2135	7	the	the	DT
cord-014597-66vd2mdu	2135	8	virus	virus	NN
cord-014597-66vd2mdu	2135	9	concentration	concentration	NN
cord-014597-66vd2mdu	2135	10	and	and	CC
cord-014597-66vd2mdu	2135	11	the	the	DT
cord-014597-66vd2mdu	2135	12	incubation	incubation	NN
cord-014597-66vd2mdu	2135	13	time	time	NN
cord-014597-66vd2mdu	2135	14	of	of	IN
cord-014597-66vd2mdu	2135	15	cells	cell	NNS
cord-014597-66vd2mdu	2135	16	with	with	IN
cord-014597-66vd2mdu	2135	17	virus	virus	NN
cord-014597-66vd2mdu	2135	18	influence	influence	NN
cord-014597-66vd2mdu	2135	19	the	the	DT
cord-014597-66vd2mdu	2135	20	transduction	transduction	NN
cord-014597-66vd2mdu	2135	21	efficiency	efficiency	NN
cord-014597-66vd2mdu	2135	22	.	.	.
cord-014597-66vd2mdu	2136	1	Based	base	VBN
cord-014597-66vd2mdu	2136	2	on	on	IN
cord-014597-66vd2mdu	2136	3	the	the	DT
cord-014597-66vd2mdu	2136	4	results	result	NNS
cord-014597-66vd2mdu	2136	5	of	of	IN
cord-014597-66vd2mdu	2136	6	the	the	DT
cord-014597-66vd2mdu	2136	7	screening	screening	NN
cord-014597-66vd2mdu	2136	8	,	,	,
cord-014597-66vd2mdu	2136	9	further	further	JJ
cord-014597-66vd2mdu	2136	10	optimization	optimization	NN
cord-014597-66vd2mdu	2136	11	of	of	IN
cord-014597-66vd2mdu	2136	12	the	the	DT
cord-014597-66vd2mdu	2136	13	transduction	transduction	NN
cord-014597-66vd2mdu	2136	14	conditions	condition	NNS
cord-014597-66vd2mdu	2136	15	will	will	MD
cord-014597-66vd2mdu	2136	16	be	be	VB
cord-014597-66vd2mdu	2136	17	done	do	VBN
cord-014597-66vd2mdu	2136	18	using	use	VBG
cord-014597-66vd2mdu	2136	19	a	a	DT
cord-014597-66vd2mdu	2136	20	face	face	NN
cord-014597-66vd2mdu	2136	21	centered	center	VBN
cord-014597-66vd2mdu	2136	22	central	central	JJ
cord-014597-66vd2mdu	2136	23	composite	composite	JJ
cord-014597-66vd2mdu	2136	24	design	design	NN
cord-014597-66vd2mdu	2136	25	with	with	IN
cord-014597-66vd2mdu	2136	26	PBS	PBS	NNP
cord-014597-66vd2mdu	2136	27	containing	contain	VBG
cord-014597-66vd2mdu	2136	28	Ca	Ca	NNP
cord-014597-66vd2mdu	2136	29	2	2	CD
cord-014597-66vd2mdu	2137	1	+	+	CC
cord-014597-66vd2mdu	2138	1	&	&	CC
cord-014597-66vd2mdu	2138	2	Mg	Mg	NNP
cord-014597-66vd2mdu	2138	3	2	2	CD
cord-014597-66vd2mdu	2138	4	+	+	SYM
cord-014597-66vd2mdu	2138	5	as	as	IN
cord-014597-66vd2mdu	2138	6	surrounding	surround	VBG
cord-014597-66vd2mdu	2138	7	fluid	fluid	NN
cord-014597-66vd2mdu	2138	8	and	and	CC
cord-014597-66vd2mdu	2138	9	at	at	IN
cord-014597-66vd2mdu	2138	10	an	an	DT
cord-014597-66vd2mdu	2138	11	incubation	incubation	NN
cord-014597-66vd2mdu	2138	12	temperature	temperature	NN
cord-014597-66vd2mdu	2138	13	of	of	IN
cord-014597-66vd2mdu	2138	14	37	37	CD
cord-014597-66vd2mdu	2138	15	°	°	NN
cord-014597-66vd2mdu	2138	16	C	c	NN
cord-014597-66vd2mdu	2138	17	.	.	.
cord-014597-66vd2mdu	2139	1	Background	background	NN
cord-014597-66vd2mdu	2139	2	Breast	breast	NN
cord-014597-66vd2mdu	2139	3	cancer	cancer	NN
cord-014597-66vd2mdu	2139	4	is	be	VBZ
cord-014597-66vd2mdu	2139	5	the	the	DT
cord-014597-66vd2mdu	2139	6	second	second	JJ
cord-014597-66vd2mdu	2139	7	main	main	JJ
cord-014597-66vd2mdu	2139	8	cause	cause	NN
cord-014597-66vd2mdu	2139	9	of	of	IN
cord-014597-66vd2mdu	2139	10	cancer	cancer	NN
cord-014597-66vd2mdu	2139	11	related	relate	VBN
cord-014597-66vd2mdu	2139	12	deaths	death	NNS
cord-014597-66vd2mdu	2139	13	for	for	IN
cord-014597-66vd2mdu	2139	14	women	woman	NNS
cord-014597-66vd2mdu	2139	15	worldwide	worldwide	RB
cord-014597-66vd2mdu	2139	16	and	and	CC
cord-014597-66vd2mdu	2139	17	among	among	IN
cord-014597-66vd2mdu	2139	18	them	-PRON-	PRP
cord-014597-66vd2mdu	2139	19	the	the	DT
cord-014597-66vd2mdu	2139	20	triple	triple	JJ
cord-014597-66vd2mdu	2139	21	negative	negative	JJ
cord-014597-66vd2mdu	2139	22	subtype	subtype	NN
cord-014597-66vd2mdu	2139	23	(	(	-LRB-
cord-014597-66vd2mdu	2139	24	TNBC	TNBC	NNP
cord-014597-66vd2mdu	2139	25	)	)	-RRB-
cord-014597-66vd2mdu	2139	26	represents	represent	VBZ
cord-014597-66vd2mdu	2139	27	a	a	DT
cord-014597-66vd2mdu	2139	28	clinical	clinical	JJ
cord-014597-66vd2mdu	2139	29	challenge	challenge	NN
cord-014597-66vd2mdu	2139	30	by	by	IN
cord-014597-66vd2mdu	2139	31	being	be	VBG
cord-014597-66vd2mdu	2139	32	associated	associate	VBN
cord-014597-66vd2mdu	2139	33	with	with	IN
cord-014597-66vd2mdu	2139	34	high	high	JJ
cord-014597-66vd2mdu	2139	35	mortality	mortality	NN
cord-014597-66vd2mdu	2139	36	and	and	CC
cord-014597-66vd2mdu	2139	37	having	have	VBG
cord-014597-66vd2mdu	2139	38	no	no	DT
cord-014597-66vd2mdu	2139	39	effective	effective	JJ
cord-014597-66vd2mdu	2139	40	therapies	therapy	NNS
cord-014597-66vd2mdu	2139	41	against	against	IN
cord-014597-66vd2mdu	2139	42	it	-PRON-	PRP
cord-014597-66vd2mdu	2139	43	[	[	-LRB-
cord-014597-66vd2mdu	2139	44	1	1	CD
cord-014597-66vd2mdu	2139	45	]	]	-RRB-
cord-014597-66vd2mdu	2139	46	,	,	,
cord-014597-66vd2mdu	2139	47	[	[	-LRB-
cord-014597-66vd2mdu	2139	48	2	2	CD
cord-014597-66vd2mdu	2139	49	]	]	-RRB-
cord-014597-66vd2mdu	2139	50	.	.	.
cord-014597-66vd2mdu	2140	1	Accordingly	accordingly	RB
cord-014597-66vd2mdu	2140	2	,	,	,
cord-014597-66vd2mdu	2140	3	there	there	EX
cord-014597-66vd2mdu	2140	4	is	be	VBZ
cord-014597-66vd2mdu	2140	5	an	an	DT
cord-014597-66vd2mdu	2140	6	urgent	urgent	JJ
cord-014597-66vd2mdu	2140	7	need	need	NN
cord-014597-66vd2mdu	2140	8	to	to	TO
cord-014597-66vd2mdu	2140	9	design	design	VB
cord-014597-66vd2mdu	2140	10	new	new	JJ
cord-014597-66vd2mdu	2140	11	and	and	CC
cord-014597-66vd2mdu	2140	12	more	more	RBR
cord-014597-66vd2mdu	2140	13	effective	effective	JJ
cord-014597-66vd2mdu	2140	14	drugs	drug	NNS
cord-014597-66vd2mdu	2140	15	to	to	TO
cord-014597-66vd2mdu	2140	16	treat	treat	VB
cord-014597-66vd2mdu	2140	17	breast	breast	NN
cord-014597-66vd2mdu	2140	18	cancer	cancer	NN
cord-014597-66vd2mdu	2140	19	.	.	.
cord-014597-66vd2mdu	2141	1	Notch	notch	NN
cord-014597-66vd2mdu	2141	2	signaling	signaling	NN
cord-014597-66vd2mdu	2141	3	is	be	VBZ
cord-014597-66vd2mdu	2141	4	an	an	DT
cord-014597-66vd2mdu	2141	5	evolutionary	evolutionary	JJ
cord-014597-66vd2mdu	2141	6	conserved	conserved	JJ
cord-014597-66vd2mdu	2141	7	cell	cell	NN
cord-014597-66vd2mdu	2141	8	-	-	HYPH
cord-014597-66vd2mdu	2141	9	to	to	IN
cord-014597-66vd2mdu	2141	10	-	-	HYPH
cord-014597-66vd2mdu	2141	11	cell	cell	NN
cord-014597-66vd2mdu	2141	12	communication	communication	NN
cord-014597-66vd2mdu	2141	13	pathway	pathway	NN
cord-014597-66vd2mdu	2141	14	crucial	crucial	JJ
cord-014597-66vd2mdu	2141	15	during	during	IN
cord-014597-66vd2mdu	2141	16	embryonic	embryonic	JJ
cord-014597-66vd2mdu	2141	17	and	and	CC
cord-014597-66vd2mdu	2141	18	breast	breast	NN
cord-014597-66vd2mdu	2141	19	development	development	NN
cord-014597-66vd2mdu	2141	20	and	and	CC
cord-014597-66vd2mdu	2141	21	tissue	tissue	NN
cord-014597-66vd2mdu	2141	22	homeostasis	homeostasis	NN
cord-014597-66vd2mdu	2141	23	.	.	.
cord-014597-66vd2mdu	2142	1	This	this	DT
cord-014597-66vd2mdu	2142	2	pathway	pathway	NN
cord-014597-66vd2mdu	2142	3	is	be	VBZ
cord-014597-66vd2mdu	2142	4	often	often	RB
cord-014597-66vd2mdu	2142	5	hyper	hyper	RB
cord-014597-66vd2mdu	2142	6	-	-	JJ
cord-014597-66vd2mdu	2142	7	activated	activate	VBN
cord-014597-66vd2mdu	2142	8	by	by	IN
cord-014597-66vd2mdu	2142	9	overexpression	overexpression	NN
cord-014597-66vd2mdu	2142	10	of	of	IN
cord-014597-66vd2mdu	2142	11	Notch	notch	NN
cord-014597-66vd2mdu	2142	12	receptors	receptor	NNS
cord-014597-66vd2mdu	2142	13	and/or	and/or	CC
cord-014597-66vd2mdu	2142	14	its	-PRON-	PRP$
cord-014597-66vd2mdu	2142	15	ligands	ligand	NNS
cord-014597-66vd2mdu	2142	16	in	in	IN
cord-014597-66vd2mdu	2142	17	several	several	JJ
cord-014597-66vd2mdu	2142	18	types	type	NNS
cord-014597-66vd2mdu	2142	19	of	of	IN
cord-014597-66vd2mdu	2142	20	cancers	cancer	NNS
cord-014597-66vd2mdu	2142	21	,	,	,
cord-014597-66vd2mdu	2142	22	such	such	JJ
cord-014597-66vd2mdu	2142	23	as	as	IN
cord-014597-66vd2mdu	2142	24	breast	breast	NN
cord-014597-66vd2mdu	2142	25	cancer	cancer	NN
cord-014597-66vd2mdu	2142	26	(	(	-LRB-
cord-014597-66vd2mdu	2142	27	TNBC	TNBC	NNP
cord-014597-66vd2mdu	2142	28	included	include	VBD
cord-014597-66vd2mdu	2142	29	)	)	-RRB-
cord-014597-66vd2mdu	2142	30	,	,	,
cord-014597-66vd2mdu	2142	31	where	where	WRB
cord-014597-66vd2mdu	2142	32	it	-PRON-	PRP
cord-014597-66vd2mdu	2142	33	contributes	contribute	VBZ
cord-014597-66vd2mdu	2142	34	to	to	IN
cord-014597-66vd2mdu	2142	35	its	-PRON-	PRP$
cord-014597-66vd2mdu	2142	36	development	development	NN
cord-014597-66vd2mdu	2142	37	,	,	,
cord-014597-66vd2mdu	2142	38	progression	progression	NN
cord-014597-66vd2mdu	2142	39	and	and	CC
cord-014597-66vd2mdu	2142	40	drug	drug	NN
cord-014597-66vd2mdu	2142	41	resistance	resistance	NN
cord-014597-66vd2mdu	2142	42	[	[	-LRB-
cord-014597-66vd2mdu	2142	43	3	3	CD
cord-014597-66vd2mdu	2142	44	]	]	-RRB-
cord-014597-66vd2mdu	2142	45	,	,	,
cord-014597-66vd2mdu	2142	46	[	[	-LRB-
cord-014597-66vd2mdu	2142	47	4	4	CD
cord-014597-66vd2mdu	2142	48	]	]	-RRB-
cord-014597-66vd2mdu	2142	49	,	,	,
cord-014597-66vd2mdu	2142	50	[	[	-LRB-
cord-014597-66vd2mdu	2142	51	5	5	CD
cord-014597-66vd2mdu	2142	52	]	]	-RRB-
cord-014597-66vd2mdu	2142	53	.	.	.
cord-014597-66vd2mdu	2143	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	2143	2	aim	aim	NN
cord-014597-66vd2mdu	2143	3	is	be	VBZ
cord-014597-66vd2mdu	2143	4	to	to	TO
cord-014597-66vd2mdu	2143	5	generate	generate	VB
cord-014597-66vd2mdu	2143	6	a	a	DT
cord-014597-66vd2mdu	2143	7	function	function	NN
cord-014597-66vd2mdu	2143	8	blocking	block	VBG
cord-014597-66vd2mdu	2143	9	antibody	antibody	NN
cord-014597-66vd2mdu	2143	10	against	against	IN
cord-014597-66vd2mdu	2143	11	the	the	DT
cord-014597-66vd2mdu	2143	12	Notch	Notch	NNP
cord-014597-66vd2mdu	2143	13	Delta	Delta	NNP
cord-014597-66vd2mdu	2143	14	-	-	HYPH
cord-014597-66vd2mdu	2143	15	like-1	like-1	NNP
cord-014597-66vd2mdu	2143	16	(	(	-LRB-
cord-014597-66vd2mdu	2143	17	DLL1	DLL1	NNP
cord-014597-66vd2mdu	2143	18	)	)	-RRB-
cord-014597-66vd2mdu	2143	19	ligand	ligand	NN
cord-014597-66vd2mdu	2143	20	with	with	IN
cord-014597-66vd2mdu	2143	21	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2143	22	efficacy	efficacy	NN
cord-014597-66vd2mdu	2143	23	against	against	IN
cord-014597-66vd2mdu	2143	24	breast	breast	NN
cord-014597-66vd2mdu	2143	25	cancer	cancer	NN
cord-014597-66vd2mdu	2143	26	.	.	.
cord-014597-66vd2mdu	2144	1	Materials	material	NNS
cord-014597-66vd2mdu	2144	2	and	and	CC
cord-014597-66vd2mdu	2144	3	methods	method	NNS
cord-014597-66vd2mdu	2144	4	DNA	dna	NN
cord-014597-66vd2mdu	2144	5	of	of	IN
cord-014597-66vd2mdu	2144	6	human	human	JJ
cord-014597-66vd2mdu	2144	7	DLL1	DLL1	NNP
cord-014597-66vd2mdu	2144	8	full	full	JJ
cord-014597-66vd2mdu	2144	9	length	length	NN
cord-014597-66vd2mdu	2144	10	extracellular	extracellular	JJ
cord-014597-66vd2mdu	2144	11	domain	domain	NN
cord-014597-66vd2mdu	2144	12	(	(	-LRB-
cord-014597-66vd2mdu	2144	13	DLL1-ECD	DLL1-ECD	NNP
cord-014597-66vd2mdu	2144	14	)	)	-RRB-
cord-014597-66vd2mdu	2144	15	and	and	CC
cord-014597-66vd2mdu	2144	16	a	a	DT
cord-014597-66vd2mdu	2144	17	truncated	truncated	JJ
cord-014597-66vd2mdu	2144	18	version	version	NN
cord-014597-66vd2mdu	2144	19	,	,	,
cord-014597-66vd2mdu	2144	20	containing	contain	VBG
cord-014597-66vd2mdu	2144	21	the	the	DT
cord-014597-66vd2mdu	2144	22	minimal	minimal	JJ
cord-014597-66vd2mdu	2144	23	binding	bind	VBG
cord-014597-66vd2mdu	2144	24	region	region	NN
cord-014597-66vd2mdu	2144	25	to	to	IN
cord-014597-66vd2mdu	2144	26	the	the	DT
cord-014597-66vd2mdu	2144	27	Notch	Notch	NNP
cord-014597-66vd2mdu	2144	28	receptor	receptor	NN
cord-014597-66vd2mdu	2144	29	(	(	-LRB-
cord-014597-66vd2mdu	2144	30	DLL1-EGF3	DLL1-EGF3	NNP
cord-014597-66vd2mdu	2144	31	)	)	-RRB-
cord-014597-66vd2mdu	2144	32	,	,	,
cord-014597-66vd2mdu	2144	33	were	be	VBD
cord-014597-66vd2mdu	2144	34	cloned	clone	VBN
cord-014597-66vd2mdu	2144	35	into	into	IN
cord-014597-66vd2mdu	2144	36	pFUSE	pfuse	NN
cord-014597-66vd2mdu	2144	37	-	-	HYPH
cord-014597-66vd2mdu	2144	38	Fc1-IgG1	fc1-igg1	NN
cord-014597-66vd2mdu	2144	39	,	,	,
cord-014597-66vd2mdu	2144	40	and	and	CC
cord-014597-66vd2mdu	2144	41	expressed	express	VBD
cord-014597-66vd2mdu	2144	42	in	in	IN
cord-014597-66vd2mdu	2144	43	HEK293E6cells	hek293e6cell	NNS
cord-014597-66vd2mdu	2144	44	.	.	.
cord-014597-66vd2mdu	2145	1	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	2145	2	proteins	protein	NNS
cord-014597-66vd2mdu	2145	3	were	be	VBD
cord-014597-66vd2mdu	2145	4	purified	purify	VBN
cord-014597-66vd2mdu	2145	5	from	from	IN
cord-014597-66vd2mdu	2145	6	culture	culture	NN
cord-014597-66vd2mdu	2145	7	media	medium	NNS
cord-014597-66vd2mdu	2145	8	by	by	IN
cord-014597-66vd2mdu	2145	9	Protein	Protein	NNP
cord-014597-66vd2mdu	2145	10	-	-	HYPH
cord-014597-66vd2mdu	2145	11	A	a	DT
cord-014597-66vd2mdu	2145	12	affinity	affinity	NN
cord-014597-66vd2mdu	2145	13	and	and	CC
cord-014597-66vd2mdu	2145	14	size	size	NN
cord-014597-66vd2mdu	2145	15	exclusion	exclusion	NN
cord-014597-66vd2mdu	2145	16	chromatography	chromatography	NN
cord-014597-66vd2mdu	2145	17	.	.	.
cord-014597-66vd2mdu	2146	1	The	the	DT
cord-014597-66vd2mdu	2146	2	human	human	JJ
cord-014597-66vd2mdu	2146	3	scFv	scFv	HYPH
cord-014597-66vd2mdu	2146	4	phage	phage	NN
cord-014597-66vd2mdu	2146	5	display	display	NN
cord-014597-66vd2mdu	2147	1	Tomlinson	Tomlinson	NNP
cord-014597-66vd2mdu	2147	2	I+J	I+J	NNP
cord-014597-66vd2mdu	2147	3	library	library	NN
cord-014597-66vd2mdu	2147	4	was	be	VBD
cord-014597-66vd2mdu	2147	5	used	use	VBN
cord-014597-66vd2mdu	2147	6	to	to	TO
cord-014597-66vd2mdu	2147	7	select	select	VB
cord-014597-66vd2mdu	2147	8	specific	specific	JJ
cord-014597-66vd2mdu	2147	9	scFv	scfv	NN
cord-014597-66vd2mdu	2147	10	against	against	IN
cord-014597-66vd2mdu	2147	11	peptides	peptide	NNS
cord-014597-66vd2mdu	2147	12	targeting	target	VBG
cord-014597-66vd2mdu	2147	13	DLL1	dll1	JJ
cord-014597-66vd2mdu	2147	14	binding	bind	VBG
cord-014597-66vd2mdu	2147	15	regions	region	NNS
cord-014597-66vd2mdu	2147	16	to	to	IN
cord-014597-66vd2mdu	2147	17	Notch	notch	NN
cord-014597-66vd2mdu	2147	18	.	.	.
cord-014597-66vd2mdu	2148	1	The	the	DT
cord-014597-66vd2mdu	2148	2	binding	bind	VBG
cord-014597-66vd2mdu	2148	3	ability	ability	NN
cord-014597-66vd2mdu	2148	4	and	and	CC
cord-014597-66vd2mdu	2148	5	specificity	specificity	NN
cord-014597-66vd2mdu	2148	6	of	of	IN
cord-014597-66vd2mdu	2148	7	the	the	DT
cord-014597-66vd2mdu	2148	8	selected	select	VBN
cord-014597-66vd2mdu	2148	9	scFv	scfv	JJ
cord-014597-66vd2mdu	2148	10	clones	clone	NNS
cord-014597-66vd2mdu	2148	11	was	be	VBD
cord-014597-66vd2mdu	2148	12	evaluated	evaluate	VBN
cord-014597-66vd2mdu	2148	13	by	by	IN
cord-014597-66vd2mdu	2148	14	scFv	scfv	NN
cord-014597-66vd2mdu	2148	15	-	-	HYPH
cord-014597-66vd2mdu	2148	16	on	on	IN
cord-014597-66vd2mdu	2148	17	-	-	HYPH
cord-014597-66vd2mdu	2148	18	Phage	phage	NN
cord-014597-66vd2mdu	2148	19	ELISA	ELISA	NNP
cord-014597-66vd2mdu	2148	20	.	.	.
cord-014597-66vd2mdu	2149	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	2149	2	strategy	strategy	NN
cord-014597-66vd2mdu	2149	3	allowed	allow	VBD
cord-014597-66vd2mdu	2149	4	us	-PRON-	PRP
cord-014597-66vd2mdu	2149	5	to	to	TO
cord-014597-66vd2mdu	2149	6	obtain	obtain	VB
cord-014597-66vd2mdu	2149	7	20	20	CD
cord-014597-66vd2mdu	2149	8	mg	mg	NNS
cord-014597-66vd2mdu	2149	9	of	of	IN
cord-014597-66vd2mdu	2149	10	pure	pure	JJ
cord-014597-66vd2mdu	2149	11	(	(	-LRB-
cord-014597-66vd2mdu	2149	12	>	>	NFP
cord-014597-66vd2mdu	2149	13	95	95	CD
cord-014597-66vd2mdu	2149	14	%	%	NN
cord-014597-66vd2mdu	2149	15	)	)	-RRB-
cord-014597-66vd2mdu	2149	16	and	and	CC
cord-014597-66vd2mdu	2149	17	stable	stable	JJ
cord-014597-66vd2mdu	2149	18	DLL1-ECD	DLL1-ECD	NNP
cord-014597-66vd2mdu	2149	19	-	-	HYPH
cord-014597-66vd2mdu	2149	20	FC	FC	NNP
cord-014597-66vd2mdu	2149	21	as	as	IN
cord-014597-66vd2mdu	2149	22	confirmed	confirm	VBN
cord-014597-66vd2mdu	2149	23	by	by	IN
cord-014597-66vd2mdu	2149	24	SDS	SDS	NNP
cord-014597-66vd2mdu	2149	25	PAGE	PAGE	NNP
cord-014597-66vd2mdu	2149	26	and	and	CC
cord-014597-66vd2mdu	2149	27	thermofluor	thermofluor	NNP
cord-014597-66vd2mdu	2149	28	assay	assay	NN
cord-014597-66vd2mdu	2149	29	.	.	.
cord-014597-66vd2mdu	2150	1	DLL1-EGF3-FC	dll1-egf3-fc	JJ
cord-014597-66vd2mdu	2150	2	yield	yield	NN
cord-014597-66vd2mdu	2150	3	was	be	VBD
cord-014597-66vd2mdu	2150	4	very	very	RB
cord-014597-66vd2mdu	2150	5	low	low	JJ
cord-014597-66vd2mdu	2150	6	and	and	CC
cord-014597-66vd2mdu	2150	7	buffer	buffer	NN
cord-014597-66vd2mdu	2150	8	screenings	screening	NNS
cord-014597-66vd2mdu	2150	9	are	be	VBP
cord-014597-66vd2mdu	2150	10	ongoing	ongoing	JJ
cord-014597-66vd2mdu	2150	11	to	to	TO
cord-014597-66vd2mdu	2150	12	optimize	optimize	VB
cord-014597-66vd2mdu	2150	13	protein	protein	NN
cord-014597-66vd2mdu	2150	14	stability	stability	NN
cord-014597-66vd2mdu	2150	15	.	.	.
cord-014597-66vd2mdu	2151	1	Functional	functional	JJ
cord-014597-66vd2mdu	2151	2	studies	study	NNS
cord-014597-66vd2mdu	2151	3	performed	perform	VBN
cord-014597-66vd2mdu	2151	4	in	in	IN
cord-014597-66vd2mdu	2151	5	human	human	JJ
cord-014597-66vd2mdu	2151	6	breast	breast	NN
cord-014597-66vd2mdu	2151	7	cancer	cancer	NN
cord-014597-66vd2mdu	2152	1	MCF7	MCF7	NNP
cord-014597-66vd2mdu	2152	2	cells	cell	NNS
cord-014597-66vd2mdu	2152	3	showed	show	VBD
cord-014597-66vd2mdu	2152	4	that	that	IN
cord-014597-66vd2mdu	2152	5	both	both	DT
cord-014597-66vd2mdu	2152	6	ligands	ligand	NNS
cord-014597-66vd2mdu	2152	7	are	be	VBP
cord-014597-66vd2mdu	2152	8	biologically	biologically	RB
cord-014597-66vd2mdu	2152	9	active	active	JJ
cord-014597-66vd2mdu	2152	10	as	as	IN
cord-014597-66vd2mdu	2152	11	they	-PRON-	PRP
cord-014597-66vd2mdu	2152	12	increased	increase	VBD
cord-014597-66vd2mdu	2152	13	the	the	DT
cord-014597-66vd2mdu	2152	14	expression	expression	NN
cord-014597-66vd2mdu	2152	15	of	of	IN
cord-014597-66vd2mdu	2152	16	the	the	DT
cord-014597-66vd2mdu	2152	17	Notch	notch	NN
cord-014597-66vd2mdu	2152	18	-	-	HYPH
cord-014597-66vd2mdu	2152	19	dependent	dependent	JJ
cord-014597-66vd2mdu	2152	20	genes	gene	NNS
cord-014597-66vd2mdu	2152	21	HES-1	HES-1	NNP
cord-014597-66vd2mdu	2152	22	,	,	,
cord-014597-66vd2mdu	2152	23	HEY	HEY	NNP
cord-014597-66vd2mdu	2152	24	-	-	HYPH
cord-014597-66vd2mdu	2152	25	L	l	NN
cord-014597-66vd2mdu	2152	26	and	and	CC
cord-014597-66vd2mdu	2152	27	HEY-1	hey-1	RB
cord-014597-66vd2mdu	2152	28	.	.	.
cord-014597-66vd2mdu	2153	1	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	2153	2	DLL1	DLL1	NNP
cord-014597-66vd2mdu	2153	3	and	and	CC
cord-014597-66vd2mdu	2153	4	peptides	peptide	NNS
cord-014597-66vd2mdu	2153	5	were	be	VBD
cord-014597-66vd2mdu	2153	6	used	use	VBN
cord-014597-66vd2mdu	2153	7	to	to	TO
cord-014597-66vd2mdu	2153	8	select	select	VB
cord-014597-66vd2mdu	2153	9	for	for	IN
cord-014597-66vd2mdu	2153	10	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	2153	11	antibodies	antibody	NNS
cord-014597-66vd2mdu	2153	12	by	by	IN
cord-014597-66vd2mdu	2153	13	Phage	Phage	NNP
cord-014597-66vd2mdu	2153	14	Display	Display	NNP
cord-014597-66vd2mdu	2153	15	.	.	.
cord-014597-66vd2mdu	2154	1	After	after	IN
cord-014597-66vd2mdu	2154	2	three	three	CD
cord-014597-66vd2mdu	2154	3	rounds	round	NNS
cord-014597-66vd2mdu	2154	4	of	of	IN
cord-014597-66vd2mdu	2154	5	panning	pan	VBG
cord-014597-66vd2mdu	2154	6	with	with	IN
cord-014597-66vd2mdu	2154	7	DLL1	DLL1	NNP
cord-014597-66vd2mdu	2154	8	peptides	peptide	NNS
cord-014597-66vd2mdu	2154	9	we	-PRON-	PRP
cord-014597-66vd2mdu	2154	10	identified	identify	VBD
cord-014597-66vd2mdu	2154	11	13	13	CD
cord-014597-66vd2mdu	2154	12	scFv	scfv	JJ
cord-014597-66vd2mdu	2154	13	positive	positive	JJ
cord-014597-66vd2mdu	2154	14	clones	clone	NNS
cord-014597-66vd2mdu	2154	15	,	,	,
cord-014597-66vd2mdu	2154	16	2	2	CD
cord-014597-66vd2mdu	2154	17	of	of	IN
cord-014597-66vd2mdu	2154	18	which	which	WDT
cord-014597-66vd2mdu	2154	19	presented	present	VBD
cord-014597-66vd2mdu	2154	20	high	high	JJ
cord-014597-66vd2mdu	2154	21	affinity	affinity	NN
cord-014597-66vd2mdu	2154	22	to	to	IN
cord-014597-66vd2mdu	2154	23	DLL1-ECD	DLL1-ECD	NNP
cord-014597-66vd2mdu	2154	24	-	-	HYPH
cord-014597-66vd2mdu	2154	25	FC	FC	NNP
cord-014597-66vd2mdu	2154	26	.	.	.
cord-014597-66vd2mdu	2155	1	Currently	currently	RB
cord-014597-66vd2mdu	2155	2	we	-PRON-	PRP
cord-014597-66vd2mdu	2155	3	are	be	VBP
cord-014597-66vd2mdu	2155	4	performing	perform	VBG
cord-014597-66vd2mdu	2155	5	more	more	JJR
cord-014597-66vd2mdu	2155	6	phage	phage	NN
cord-014597-66vd2mdu	2155	7	display	display	NN
cord-014597-66vd2mdu	2155	8	selections	selection	NNS
cord-014597-66vd2mdu	2155	9	to	to	TO
cord-014597-66vd2mdu	2155	10	increase	increase	VB
cord-014597-66vd2mdu	2155	11	the	the	DT
cord-014597-66vd2mdu	2155	12	number	number	NN
cord-014597-66vd2mdu	2155	13	of	of	IN
cord-014597-66vd2mdu	2155	14	positive	positive	JJ
cord-014597-66vd2mdu	2155	15	clones	clone	NNS
cord-014597-66vd2mdu	2155	16	.	.	.
cord-014597-66vd2mdu	2156	1	scFv	scFv	NNP
cord-014597-66vd2mdu	2156	2	with	with	IN
cord-014597-66vd2mdu	2156	3	higher	high	JJR
cord-014597-66vd2mdu	2156	4	affinities	affinity	NNS
cord-014597-66vd2mdu	2156	5	will	will	MD
cord-014597-66vd2mdu	2156	6	be	be	VB
cord-014597-66vd2mdu	2156	7	reformatted	reformatte	VBN
cord-014597-66vd2mdu	2156	8	into	into	IN
cord-014597-66vd2mdu	2156	9	IgGs	IgGs	NNP
cord-014597-66vd2mdu	2156	10	and	and	CC
cord-014597-66vd2mdu	2156	11	their	-PRON-	PRP$
cord-014597-66vd2mdu	2156	12	ability	ability	NN
cord-014597-66vd2mdu	2156	13	to	to	TO
cord-014597-66vd2mdu	2156	14	inhibit	inhibit	VB
cord-014597-66vd2mdu	2156	15	the	the	DT
cord-014597-66vd2mdu	2156	16	Notch	Notch	NNP
cord-014597-66vd2mdu	2156	17	pathway	pathway	NN
cord-014597-66vd2mdu	2156	18	will	will	MD
cord-014597-66vd2mdu	2156	19	be	be	VB
cord-014597-66vd2mdu	2156	20	evaluated	evaluate	VBN
cord-014597-66vd2mdu	2156	21	.	.	.
cord-014597-66vd2mdu	2157	1	The	the	DT
cord-014597-66vd2mdu	2157	2	anti	anti	JJ
cord-014597-66vd2mdu	2157	3	-	-	JJ
cord-014597-66vd2mdu	2157	4	oncogenic	oncogenic	JJ
cord-014597-66vd2mdu	2157	5	effects	effect	NNS
cord-014597-66vd2mdu	2157	6	of	of	IN
cord-014597-66vd2mdu	2157	7	anti	anti	JJ
cord-014597-66vd2mdu	2157	8	-	-	JJ
cord-014597-66vd2mdu	2157	9	DLL1	dll1	JJ
cord-014597-66vd2mdu	2157	10	IgGs	iggs	NN
cord-014597-66vd2mdu	2157	11	will	will	MD
cord-014597-66vd2mdu	2157	12	be	be	VB
cord-014597-66vd2mdu	2157	13	assessed	assess	VBN
cord-014597-66vd2mdu	2157	14	in	in	IN
cord-014597-66vd2mdu	2157	15	breast	breast	NN
cord-014597-66vd2mdu	2157	16	cancer	cancer	NN
cord-014597-66vd2mdu	2157	17	cells	cell	NNS
cord-014597-66vd2mdu	2157	18	in	in	IN
cord-014597-66vd2mdu	2157	19	viability	viability	NN
cord-014597-66vd2mdu	2157	20	/	/	SYM
cord-014597-66vd2mdu	2157	21	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	2157	22	,	,	,
cord-014597-66vd2mdu	2157	23	proliferation	proliferation	NN
cord-014597-66vd2mdu	2157	24	,	,	,
cord-014597-66vd2mdu	2157	25	migration	migration	NN
cord-014597-66vd2mdu	2157	26	,	,	,
cord-014597-66vd2mdu	2157	27	and	and	CC
cord-014597-66vd2mdu	2157	28	invasion	invasion	NN
cord-014597-66vd2mdu	2157	29	assays	assay	NNS
cord-014597-66vd2mdu	2157	30	.	.	.
cord-014597-66vd2mdu	2158	1	An	an	DT
cord-014597-66vd2mdu	2158	2	anti	anti	JJ
cord-014597-66vd2mdu	2158	3	-	-	JJ
cord-014597-66vd2mdu	2158	4	DLL1	dll1	JJ
cord-014597-66vd2mdu	2158	5	IgG	igg	NN
cord-014597-66vd2mdu	2158	6	with	with	IN
cord-014597-66vd2mdu	2158	7	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2158	8	efficacy	efficacy	NN
cord-014597-66vd2mdu	2158	9	against	against	IN
cord-014597-66vd2mdu	2158	10	breast	breast	NN
cord-014597-66vd2mdu	2158	11	cancer	cancer	NN
cord-014597-66vd2mdu	2158	12	will	will	MD
cord-014597-66vd2mdu	2158	13	demonstrate	demonstrate	VB
cord-014597-66vd2mdu	2158	14	that	that	IN
cord-014597-66vd2mdu	2158	15	targeting	target	VBG
cord-014597-66vd2mdu	2158	16	DLL1	DLL1	NNP
cord-014597-66vd2mdu	2158	17	could	could	MD
cord-014597-66vd2mdu	2158	18	be	be	VB
cord-014597-66vd2mdu	2158	19	one	one	CD
cord-014597-66vd2mdu	2158	20	of	of	IN
cord-014597-66vd2mdu	2158	21	the	the	DT
cord-014597-66vd2mdu	2158	22	key	key	JJ
cord-014597-66vd2mdu	2158	23	factors	factor	NNS
cord-014597-66vd2mdu	2158	24	for	for	IN
cord-014597-66vd2mdu	2158	25	successfully	successfully	RB
cord-014597-66vd2mdu	2158	26	targeting	target	VBG
cord-014597-66vd2mdu	2158	27	breast	breast	NN
cord-014597-66vd2mdu	2158	28	cancer	cancer	NN
cord-014597-66vd2mdu	2158	29	.	.	.
cord-014597-66vd2mdu	2159	1	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	2159	2	adeno	adeno	NN
cord-014597-66vd2mdu	2159	3	-	-	HYPH
cord-014597-66vd2mdu	2159	4	associated	associate	VBN
cord-014597-66vd2mdu	2159	5	virus	virus	NN
cord-014597-66vd2mdu	2159	6	(	(	-LRB-
cord-014597-66vd2mdu	2159	7	rAAV	rAAV	NNP
cord-014597-66vd2mdu	2159	8	)	)	-RRB-
cord-014597-66vd2mdu	2159	9	approaches	approach	NNS
cord-014597-66vd2mdu	2159	10	have	have	VBP
cord-014597-66vd2mdu	2159	11	an	an	DT
cord-014597-66vd2mdu	2159	12	outstanding	outstanding	JJ
cord-014597-66vd2mdu	2159	13	reputation	reputation	NN
cord-014597-66vd2mdu	2159	14	in	in	IN
cord-014597-66vd2mdu	2159	15	gene	gene	NN
cord-014597-66vd2mdu	2159	16	therapy	therapy	NN
cord-014597-66vd2mdu	2159	17	and	and	CC
cord-014597-66vd2mdu	2159	18	are	be	VBP
cord-014597-66vd2mdu	2159	19	evaluated	evaluate	VBN
cord-014597-66vd2mdu	2159	20	for	for	IN
cord-014597-66vd2mdu	2159	21	cancer	cancer	NN
cord-014597-66vd2mdu	2159	22	therapy	therapy	NN
cord-014597-66vd2mdu	2159	23	[	[	-LRB-
cord-014597-66vd2mdu	2159	24	1	1	CD
cord-014597-66vd2mdu	2159	25	]	]	-RRB-
cord-014597-66vd2mdu	2159	26	.	.	.
cord-014597-66vd2mdu	2160	1	Advantages	advantage	NNS
cord-014597-66vd2mdu	2160	2	include	include	VBP
cord-014597-66vd2mdu	2160	3	long	long	JJ
cord-014597-66vd2mdu	2160	4	-	-	HYPH
cord-014597-66vd2mdu	2160	5	term	term	NN
cord-014597-66vd2mdu	2160	6	gene	gene	NN
cord-014597-66vd2mdu	2160	7	expression	expression	NN
cord-014597-66vd2mdu	2160	8	,	,	,
cord-014597-66vd2mdu	2160	9	targeting	target	VBG
cord-014597-66vd2mdu	2160	10	of	of	IN
cord-014597-66vd2mdu	2160	11	dividing	divide	VBG
cord-014597-66vd2mdu	2160	12	and	and	CC
cord-014597-66vd2mdu	2160	13	non	non	JJ
cord-014597-66vd2mdu	2160	14	-	-	JJ
cord-014597-66vd2mdu	2160	15	dividing	dividing	JJ
cord-014597-66vd2mdu	2160	16	cells	cell	NNS
cord-014597-66vd2mdu	2160	17	,	,	,
cord-014597-66vd2mdu	2160	18	and	and	CC
cord-014597-66vd2mdu	2160	19	low	low	JJ
cord-014597-66vd2mdu	2160	20	immunogenicity	immunogenicity	NN
cord-014597-66vd2mdu	2160	21	.	.	.
cord-014597-66vd2mdu	2161	1	Established	established	JJ
cord-014597-66vd2mdu	2161	2	rAAV	raav	NN
cord-014597-66vd2mdu	2161	3	production	production	NN
cord-014597-66vd2mdu	2161	4	utilizes	utilize	VBZ
cord-014597-66vd2mdu	2161	5	triple	triple	JJ
cord-014597-66vd2mdu	2161	6	transfection	transfection	NN
cord-014597-66vd2mdu	2161	7	of	of	IN
cord-014597-66vd2mdu	2161	8	adherent	adherent	JJ
cord-014597-66vd2mdu	2161	9	HEK	HEK	NNP
cord-014597-66vd2mdu	2161	10	293	293	CD
cord-014597-66vd2mdu	2161	11	cells	cell	NNS
cord-014597-66vd2mdu	2161	12	,	,	,
cord-014597-66vd2mdu	2161	13	which	which	WDT
cord-014597-66vd2mdu	2161	14	hardly	hardly	RB
cord-014597-66vd2mdu	2161	15	meets	meet	VBZ
cord-014597-66vd2mdu	2161	16	product	product	NN
cord-014597-66vd2mdu	2161	17	yield	yield	NN
cord-014597-66vd2mdu	2161	18	requirements	requirement	NNS
cord-014597-66vd2mdu	2161	19	for	for	IN
cord-014597-66vd2mdu	2161	20	clinical	clinical	JJ
cord-014597-66vd2mdu	2161	21	applications	application	NNS
cord-014597-66vd2mdu	2161	22	.	.	.
cord-014597-66vd2mdu	2162	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2162	2	transferred	transfer	VBD
cord-014597-66vd2mdu	2162	3	the	the	DT
cord-014597-66vd2mdu	2162	4	AAV	AAV	NNP
cord-014597-66vd2mdu	2162	5	production	production	NN
cord-014597-66vd2mdu	2162	6	system	system	NN
cord-014597-66vd2mdu	2162	7	to	to	IN
cord-014597-66vd2mdu	2162	8	HEK	HEK	NNP
cord-014597-66vd2mdu	2162	9	293-F	293-F	NNP
cord-014597-66vd2mdu	2162	10	suspension	suspension	NN
cord-014597-66vd2mdu	2162	11	cells	cell	NNS
cord-014597-66vd2mdu	2162	12	.	.	.
cord-014597-66vd2mdu	2163	1	This	this	DT
cord-014597-66vd2mdu	2163	2	process	process	NN
cord-014597-66vd2mdu	2163	3	is	be	VBZ
cord-014597-66vd2mdu	2163	4	scalable	scalable	JJ
cord-014597-66vd2mdu	2163	5	and	and	CC
cord-014597-66vd2mdu	2163	6	uses	use	VBZ
cord-014597-66vd2mdu	2163	7	serum	serum	NN
cord-014597-66vd2mdu	2163	8	-	-	HYPH
cord-014597-66vd2mdu	2163	9	free	free	JJ
cord-014597-66vd2mdu	2163	10	media	medium	NNS
cord-014597-66vd2mdu	2163	11	streamlining	streamline	VBG
cord-014597-66vd2mdu	2163	12	downstream	downstream	JJ
cord-014597-66vd2mdu	2163	13	procedures	procedure	NNS
cord-014597-66vd2mdu	2163	14	.	.	.
cord-014597-66vd2mdu	2164	1	After	after	IN
cord-014597-66vd2mdu	2164	2	optimization	optimization	NN
cord-014597-66vd2mdu	2164	3	of	of	IN
cord-014597-66vd2mdu	2164	4	transfection	transfection	NN
cord-014597-66vd2mdu	2164	5	efficiencies	efficiency	NNS
cord-014597-66vd2mdu	2164	6	and	and	CC
cord-014597-66vd2mdu	2164	7	shaker	shaker	NN
cord-014597-66vd2mdu	2164	8	cultivations	cultivation	NNS
cord-014597-66vd2mdu	2164	9	,	,	,
cord-014597-66vd2mdu	2164	10	we	-PRON-	PRP
cord-014597-66vd2mdu	2164	11	produced	produce	VBD
cord-014597-66vd2mdu	2164	12	titers	titer	NNS
cord-014597-66vd2mdu	2164	13	of	of	IN
cord-014597-66vd2mdu	2164	14	1×10	1×10	CD
cord-014597-66vd2mdu	2164	15	5	5	CD
cord-014597-66vd2mdu	2164	16	viral	viral	JJ
cord-014597-66vd2mdu	2164	17	genomes	genome	NNS
cord-014597-66vd2mdu	2164	18	per	per	IN
cord-014597-66vd2mdu	2164	19	cell	cell	NN
cord-014597-66vd2mdu	2164	20	in	in	IN
cord-014597-66vd2mdu	2164	21	a	a	DT
cord-014597-66vd2mdu	2164	22	2	2	CD
cord-014597-66vd2mdu	2164	23	l	l	NN
cord-014597-66vd2mdu	2164	24	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2164	25	.	.	.
cord-014597-66vd2mdu	2165	1	The	the	DT
cord-014597-66vd2mdu	2165	2	suspension	suspension	NN
cord-014597-66vd2mdu	2165	3	adapted	adapt	VBD
cord-014597-66vd2mdu	2165	4	HEK	HEK	NNP
cord-014597-66vd2mdu	2165	5	-	-	HYPH
cord-014597-66vd2mdu	2165	6	FreeStyle	FreeStyle	NNP
cord-014597-66vd2mdu	2165	7	293-F	293-f	CD
cord-014597-66vd2mdu	2165	8	cell	cell	NN
cord-014597-66vd2mdu	2165	9	line	line	NN
cord-014597-66vd2mdu	2165	10	was	be	VBD
cord-014597-66vd2mdu	2165	11	used	use	VBN
cord-014597-66vd2mdu	2165	12	for	for	IN
cord-014597-66vd2mdu	2165	13	the	the	DT
cord-014597-66vd2mdu	2165	14	experiments	experiment	NNS
cord-014597-66vd2mdu	2165	15	in	in	IN
cord-014597-66vd2mdu	2165	16	chemically	chemically	RB
cord-014597-66vd2mdu	2165	17	defined	define	VBN
cord-014597-66vd2mdu	2165	18	animal	animal	NN
cord-014597-66vd2mdu	2165	19	component	component	NN
cord-014597-66vd2mdu	2165	20	free	free	JJ
cord-014597-66vd2mdu	2165	21	media	medium	NNS
cord-014597-66vd2mdu	2165	22	(	(	-LRB-
cord-014597-66vd2mdu	2165	23	HEK	HEK	NNP
cord-014597-66vd2mdu	2165	24	-	-	HYPH
cord-014597-66vd2mdu	2165	25	TF	TF	NNP
cord-014597-66vd2mdu	2165	26	,	,	,
cord-014597-66vd2mdu	2165	27	HEK	HEK	NNP
cord-014597-66vd2mdu	2165	28	-	-	HYPH
cord-014597-66vd2mdu	2165	29	GM	GM	NNP
cord-014597-66vd2mdu	2165	30	(	(	-LRB-
cord-014597-66vd2mdu	2165	31	Xell	Xell	NNP
cord-014597-66vd2mdu	2165	32	AG	AG	NNP
cord-014597-66vd2mdu	2165	33	)	)	-RRB-
cord-014597-66vd2mdu	2165	34	,	,	,
cord-014597-66vd2mdu	2165	35	Freestyle	Freestyle	NNP
cord-014597-66vd2mdu	2165	36	F17	F17	NNP
cord-014597-66vd2mdu	2165	37	(	(	-LRB-
cord-014597-66vd2mdu	2165	38	Thermo	Thermo	NNP
cord-014597-66vd2mdu	2165	39	Fisher	Fisher	NNP
cord-014597-66vd2mdu	2165	40	Scientific	Scientific	NNP
cord-014597-66vd2mdu	2165	41	)	)	-RRB-
cord-014597-66vd2mdu	2165	42	)	)	-RRB-
cord-014597-66vd2mdu	2165	43	.	.	.
cord-014597-66vd2mdu	2166	1	Samples	sample	NNS
cord-014597-66vd2mdu	2166	2	for	for	IN
cord-014597-66vd2mdu	2166	3	viable	viable	JJ
cord-014597-66vd2mdu	2166	4	cell	cell	NN
cord-014597-66vd2mdu	2166	5	density	density	NN
cord-014597-66vd2mdu	2166	6	and	and	CC
cord-014597-66vd2mdu	2166	7	viabilities	viability	NNS
cord-014597-66vd2mdu	2166	8	were	be	VBD
cord-014597-66vd2mdu	2166	9	taken	take	VBN
cord-014597-66vd2mdu	2166	10	daily	daily	RB
cord-014597-66vd2mdu	2166	11	and	and	CC
cord-014597-66vd2mdu	2166	12	analyzed	analyze	VBD
cord-014597-66vd2mdu	2166	13	using	use	VBG
cord-014597-66vd2mdu	2166	14	an	an	DT
cord-014597-66vd2mdu	2166	15	automated	automate	VBN
cord-014597-66vd2mdu	2166	16	cell	cell	NN
cord-014597-66vd2mdu	2166	17	counting	counting	NN
cord-014597-66vd2mdu	2166	18	system	system	NN
cord-014597-66vd2mdu	2166	19	(	(	-LRB-
cord-014597-66vd2mdu	2166	20	CEDEX	CEDEX	NNP
cord-014597-66vd2mdu	2166	21	,	,	,
cord-014597-66vd2mdu	2166	22	Roche	Roche	NNP
cord-014597-66vd2mdu	2166	23	Diagnostics	Diagnostics	NNP
cord-014597-66vd2mdu	2166	24	)	)	-RRB-
cord-014597-66vd2mdu	2166	25	.	.	.
cord-014597-66vd2mdu	2167	1	Transient	transient	JJ
cord-014597-66vd2mdu	2167	2	transfection	transfection	NN
cord-014597-66vd2mdu	2167	3	of	of	IN
cord-014597-66vd2mdu	2167	4	3×10	3×10	CD
cord-014597-66vd2mdu	2167	5	6	6	CD
cord-014597-66vd2mdu	2167	6	cells	cell	NNS
cord-014597-66vd2mdu	2167	7	/	/	SYM
cord-014597-66vd2mdu	2167	8	ml	ml	NNS
cord-014597-66vd2mdu	2167	9	was	be	VBD
cord-014597-66vd2mdu	2167	10	carried	carry	VBN
cord-014597-66vd2mdu	2167	11	out	out	RP
cord-014597-66vd2mdu	2167	12	with	with	IN
cord-014597-66vd2mdu	2167	13	polyethylenimine	polyethylenimine	NNP
cord-014597-66vd2mdu	2167	14	Max	Max	NNP
cord-014597-66vd2mdu	2167	15	in	in	IN
cord-014597-66vd2mdu	2167	16	a	a	DT
cord-014597-66vd2mdu	2167	17	1:4	1:4	CD
cord-014597-66vd2mdu	2167	18	DNA	DNA	NNP
cord-014597-66vd2mdu	2167	19	-	-	HYPH
cord-014597-66vd2mdu	2167	20	PEI	PEI	NNP
cord-014597-66vd2mdu	2167	21	ratio	ratio	NN
cord-014597-66vd2mdu	2167	22	(	(	-LRB-
cord-014597-66vd2mdu	2167	23	w	w	NNP
cord-014597-66vd2mdu	2167	24	/	/	SYM
cord-014597-66vd2mdu	2167	25	w	w	NN
cord-014597-66vd2mdu	2167	26	)	)	-RRB-
cord-014597-66vd2mdu	2167	27	with	with	IN
cord-014597-66vd2mdu	2167	28	2	2	CD
cord-014597-66vd2mdu	2167	29	μg	μg	NN
cord-014597-66vd2mdu	2167	30	DNA	DNA	NNP
cord-014597-66vd2mdu	2167	31	.	.	.
cord-014597-66vd2mdu	2168	1	Three	three	CD
cord-014597-66vd2mdu	2168	2	plasmids	plasmid	NNS
cord-014597-66vd2mdu	2168	3	(	(	-LRB-
cord-014597-66vd2mdu	2168	4	pGOI	pgoi	FW
cord-014597-66vd2mdu	2168	5	,	,	,
cord-014597-66vd2mdu	2168	6	pRepCap	pRepCap	NNP
cord-014597-66vd2mdu	2168	7	,	,	,
cord-014597-66vd2mdu	2168	8	pHelper	pHelper	NNP
cord-014597-66vd2mdu	2168	9	)	)	-RRB-
cord-014597-66vd2mdu	2168	10	were	be	VBD
cord-014597-66vd2mdu	2168	11	applied	apply	VBN
cord-014597-66vd2mdu	2168	12	in	in	IN
cord-014597-66vd2mdu	2168	13	a	a	DT
cord-014597-66vd2mdu	2168	14	molar	molar	JJ
cord-014597-66vd2mdu	2168	15	1:1:1	1:1:1	CD
cord-014597-66vd2mdu	2168	16	ratio	ratio	NN
cord-014597-66vd2mdu	2168	17	(	(	-LRB-
cord-014597-66vd2mdu	2168	18	Fig	Fig	NNP
cord-014597-66vd2mdu	2168	19	.	.	.
cord-014597-66vd2mdu	2168	20	1a	1a	CD
cord-014597-66vd2mdu	2168	21	)	)	-RRB-
cord-014597-66vd2mdu	2168	22	.	.	.
cord-014597-66vd2mdu	2169	1	Pretests	pretest	NNS
cord-014597-66vd2mdu	2169	2	were	be	VBD
cord-014597-66vd2mdu	2169	3	performed	perform	VBN
cord-014597-66vd2mdu	2169	4	in	in	IN
cord-014597-66vd2mdu	2169	5	orbital	orbital	JJ
cord-014597-66vd2mdu	2169	6	shaking	shake	VBG
cord-014597-66vd2mdu	2169	7	tube	tube	NN
cord-014597-66vd2mdu	2169	8	spin	spin	NN
cord-014597-66vd2mdu	2169	9	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	2169	10	.	.	.
cord-014597-66vd2mdu	2170	1	For	for	IN
cord-014597-66vd2mdu	2170	2	scale	scale	NN
cord-014597-66vd2mdu	2170	3	-	-	HYPH
cord-014597-66vd2mdu	2170	4	up	up	RP
cord-014597-66vd2mdu	2170	5	,	,	,
cord-014597-66vd2mdu	2170	6	batch	batch	NN
cord-014597-66vd2mdu	2170	7	processes	process	NNS
cord-014597-66vd2mdu	2170	8	were	be	VBD
cord-014597-66vd2mdu	2170	9	carried	carry	VBN
cord-014597-66vd2mdu	2170	10	out	out	RP
cord-014597-66vd2mdu	2170	11	in	in	IN
cord-014597-66vd2mdu	2170	12	125	125	CD
cord-014597-66vd2mdu	2170	13	ml	ml	NN
cord-014597-66vd2mdu	2170	14	shake	shake	NN
cord-014597-66vd2mdu	2170	15	flasks	flask	NNS
cord-014597-66vd2mdu	2170	16	as	as	RB
cord-014597-66vd2mdu	2170	17	well	well	RB
cord-014597-66vd2mdu	2170	18	as	as	IN
cord-014597-66vd2mdu	2170	19	in	in	IN
cord-014597-66vd2mdu	2170	20	2	2	CD
cord-014597-66vd2mdu	2170	21	l	l	NN
cord-014597-66vd2mdu	2170	22	stirred	stir	VBD
cord-014597-66vd2mdu	2170	23	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	2170	24	at	at	IN
cord-014597-66vd2mdu	2170	25	30	30	CD
cord-014597-66vd2mdu	2170	26	%	%	NN
cord-014597-66vd2mdu	2170	27	air	air	NN
cord-014597-66vd2mdu	2170	28	saturation	saturation	NN
cord-014597-66vd2mdu	2170	29	and	and	CC
cord-014597-66vd2mdu	2170	30	pH	ph	NN
cord-014597-66vd2mdu	2170	31	7.1	7.1	CD
cord-014597-66vd2mdu	2170	32	.	.	.
cord-014597-66vd2mdu	2171	1	Transfection	transfection	NN
cord-014597-66vd2mdu	2171	2	efficiencies	efficiency	NNS
cord-014597-66vd2mdu	2171	3	and	and	CC
cord-014597-66vd2mdu	2171	4	rAAV	raav	NN
cord-014597-66vd2mdu	2171	5	production	production	NN
cord-014597-66vd2mdu	2171	6	were	be	VBD
cord-014597-66vd2mdu	2171	7	quantified	quantify	VBN
cord-014597-66vd2mdu	2171	8	by	by	IN
cord-014597-66vd2mdu	2171	9	flow	flow	NN
cord-014597-66vd2mdu	2171	10	cytometry	cytometry	NN
cord-014597-66vd2mdu	2171	11	using	use	VBG
cord-014597-66vd2mdu	2171	12	a	a	DT
cord-014597-66vd2mdu	2171	13	GOI	GOI	NNP
cord-014597-66vd2mdu	2171	14	coding	code	VBG
cord-014597-66vd2mdu	2171	15	for	for	IN
cord-014597-66vd2mdu	2171	16	a	a	DT
cord-014597-66vd2mdu	2171	17	fluorescent	fluorescent	JJ
cord-014597-66vd2mdu	2171	18	protein	protein	NN
cord-014597-66vd2mdu	2171	19	and	and	CC
cord-014597-66vd2mdu	2171	20	qPCR	qpcr	NN
cord-014597-66vd2mdu	2171	21	of	of	IN
cord-014597-66vd2mdu	2171	22	genomic	genomic	JJ
cord-014597-66vd2mdu	2171	23	copies	copy	NNS
cord-014597-66vd2mdu	2171	24	,	,	,
cord-014597-66vd2mdu	2171	25	respectively	respectively	RB
cord-014597-66vd2mdu	2171	26	.	.	.
cord-014597-66vd2mdu	2172	1	By	by	IN
cord-014597-66vd2mdu	2172	2	optimizing	optimize	VBG
cord-014597-66vd2mdu	2172	3	the	the	DT
cord-014597-66vd2mdu	2172	4	DNA	dna	NN
cord-014597-66vd2mdu	2172	5	amount	amount	NN
cord-014597-66vd2mdu	2172	6	for	for	IN
cord-014597-66vd2mdu	2172	7	transfection	transfection	NN
cord-014597-66vd2mdu	2172	8	of	of	IN
cord-014597-66vd2mdu	2172	9	293-F	293-f	CD
cord-014597-66vd2mdu	2172	10	cells	cell	NNS
cord-014597-66vd2mdu	2172	11	more	more	JJR
cord-014597-66vd2mdu	2172	12	than	than	IN
cord-014597-66vd2mdu	2172	13	90	90	CD
cord-014597-66vd2mdu	2172	14	%	%	NN
cord-014597-66vd2mdu	2172	15	of	of	IN
cord-014597-66vd2mdu	2172	16	the	the	DT
cord-014597-66vd2mdu	2172	17	cells	cell	NNS
cord-014597-66vd2mdu	2172	18	were	be	VBD
cord-014597-66vd2mdu	2172	19	reproducibly	reproducibly	RB
cord-014597-66vd2mdu	2172	20	transfected	transfecte	VBN
cord-014597-66vd2mdu	2172	21	.	.	.
cord-014597-66vd2mdu	2173	1	Batch	batch	NN
cord-014597-66vd2mdu	2173	2	cultivations	cultivation	NNS
cord-014597-66vd2mdu	2173	3	in	in	IN
cord-014597-66vd2mdu	2173	4	shaker	shaker	NN
cord-014597-66vd2mdu	2173	5	flasks	flask	NNS
cord-014597-66vd2mdu	2173	6	revealed	reveal	VBD
cord-014597-66vd2mdu	2173	7	that	that	IN
cord-014597-66vd2mdu	2173	8	rAAV	rAAV	NNP
cord-014597-66vd2mdu	2173	9	were	be	VBD
cord-014597-66vd2mdu	2173	10	produced	produce	VBN
cord-014597-66vd2mdu	2173	11	in	in	IN
cord-014597-66vd2mdu	2173	12	the	the	DT
cord-014597-66vd2mdu	2173	13	first	first	JJ
cord-014597-66vd2mdu	2173	14	24	24	CD
cord-014597-66vd2mdu	2173	15	-	-	SYM
cord-014597-66vd2mdu	2173	16	96	96	CD
cord-014597-66vd2mdu	2173	17	h	h	NN
cord-014597-66vd2mdu	2173	18	after	after	IN
cord-014597-66vd2mdu	2173	19	transfection	transfection	NN
cord-014597-66vd2mdu	2173	20	.	.	.
cord-014597-66vd2mdu	2174	1	Figure	figure	NN
cord-014597-66vd2mdu	2174	2	1b	1b	CD
cord-014597-66vd2mdu	2174	3	shows	show	VBZ
cord-014597-66vd2mdu	2174	4	viable	viable	JJ
cord-014597-66vd2mdu	2174	5	cell	cell	NN
cord-014597-66vd2mdu	2174	6	densities	density	NNS
cord-014597-66vd2mdu	2174	7	and	and	CC
cord-014597-66vd2mdu	2174	8	viabilities	viability	NNS
cord-014597-66vd2mdu	2174	9	in	in	IN
cord-014597-66vd2mdu	2174	10	relation	relation	NN
cord-014597-66vd2mdu	2174	11	to	to	IN
cord-014597-66vd2mdu	2174	12	the	the	DT
cord-014597-66vd2mdu	2174	13	genomic	genomic	JJ
cord-014597-66vd2mdu	2174	14	titer	titer	NN
cord-014597-66vd2mdu	2174	15	.	.	.
cord-014597-66vd2mdu	2175	1	Genomic	genomic	JJ
cord-014597-66vd2mdu	2175	2	titers	titer	NNS
cord-014597-66vd2mdu	2175	3	were	be	VBD
cord-014597-66vd2mdu	2175	4	determined	determine	VBN
cord-014597-66vd2mdu	2175	5	from	from	IN
cord-014597-66vd2mdu	2175	6	raw	raw	JJ
cord-014597-66vd2mdu	2175	7	cell	cell	NN
cord-014597-66vd2mdu	2175	8	extracts	extract	NNS
cord-014597-66vd2mdu	2175	9	and	and	CC
cord-014597-66vd2mdu	2175	10	up	up	IN
cord-014597-66vd2mdu	2175	11	to	to	TO
cord-014597-66vd2mdu	2175	12	10	10	CD
cord-014597-66vd2mdu	2175	13	9	9	CD
cord-014597-66vd2mdu	2175	14	copies	copy	NNS
cord-014597-66vd2mdu	2175	15	/	/	SYM
cord-014597-66vd2mdu	2175	16	ml	ml	NNS
cord-014597-66vd2mdu	2175	17	were	be	VBD
cord-014597-66vd2mdu	2175	18	repetitively	repetitively	RB
cord-014597-66vd2mdu	2175	19	achievable	achievable	JJ
cord-014597-66vd2mdu	2175	20	.	.	.
cord-014597-66vd2mdu	2176	1	A	a	DT
cord-014597-66vd2mdu	2176	2	decrease	decrease	NN
cord-014597-66vd2mdu	2176	3	in	in	IN
cord-014597-66vd2mdu	2176	4	viability	viability	NN
cord-014597-66vd2mdu	2176	5	marked	mark	VBD
cord-014597-66vd2mdu	2176	6	the	the	DT
cord-014597-66vd2mdu	2176	7	decline	decline	NN
cord-014597-66vd2mdu	2176	8	in	in	IN
cord-014597-66vd2mdu	2176	9	genomic	genomic	JJ
cord-014597-66vd2mdu	2176	10	copies	copy	NNS
cord-014597-66vd2mdu	2176	11	per	per	IN
cord-014597-66vd2mdu	2176	12	ml	ml	NN
cord-014597-66vd2mdu	2176	13	showing	show	VBG
cord-014597-66vd2mdu	2176	14	that	that	IN
cord-014597-66vd2mdu	2176	15	a	a	DT
cord-014597-66vd2mdu	2176	16	prolongation	prolongation	NN
cord-014597-66vd2mdu	2176	17	of	of	IN
cord-014597-66vd2mdu	2176	18	the	the	DT
cord-014597-66vd2mdu	2176	19	process	process	NN
cord-014597-66vd2mdu	2176	20	e.g.	e.g.	RB
cord-014597-66vd2mdu	2176	21	by	by	IN
cord-014597-66vd2mdu	2176	22	addition	addition	NN
cord-014597-66vd2mdu	2176	23	of	of	IN
cord-014597-66vd2mdu	2176	24	a	a	DT
cord-014597-66vd2mdu	2176	25	feed	feed	NN
cord-014597-66vd2mdu	2176	26	would	would	MD
cord-014597-66vd2mdu	2176	27	probably	probably	RB
cord-014597-66vd2mdu	2176	28	not	not	RB
cord-014597-66vd2mdu	2176	29	increase	increase	VB
cord-014597-66vd2mdu	2176	30	yield	yield	NN
cord-014597-66vd2mdu	2176	31	.	.	.
cord-014597-66vd2mdu	2177	1	In	in	IN
cord-014597-66vd2mdu	2177	2	a	a	DT
cord-014597-66vd2mdu	2177	3	first	first	JJ
cord-014597-66vd2mdu	2177	4	scale	scale	NN
cord-014597-66vd2mdu	2177	5	-	-	HYPH
cord-014597-66vd2mdu	2177	6	up	up	NN
cord-014597-66vd2mdu	2177	7	,	,	,
cord-014597-66vd2mdu	2177	8	the	the	DT
cord-014597-66vd2mdu	2177	9	rAAV	rAAV	NNP
cord-014597-66vd2mdu	2177	10	production	production	NN
cord-014597-66vd2mdu	2177	11	was	be	VBD
cord-014597-66vd2mdu	2177	12	transferred	transfer	VBN
cord-014597-66vd2mdu	2177	13	to	to	IN
cord-014597-66vd2mdu	2177	14	a	a	DT
cord-014597-66vd2mdu	2177	15	2	2	CD
cord-014597-66vd2mdu	2177	16	l	l	NN
cord-014597-66vd2mdu	2177	17	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2177	18	(	(	-LRB-
cord-014597-66vd2mdu	2177	19	Fig	Fig	NNP
cord-014597-66vd2mdu	2177	20	.	.	.
cord-014597-66vd2mdu	2177	21	1c	1c	CD
cord-014597-66vd2mdu	2177	22	)	)	-RRB-
cord-014597-66vd2mdu	2177	23	.	.	.
cord-014597-66vd2mdu	2178	1	Transfection	transfection	NN
cord-014597-66vd2mdu	2178	2	efficiencies	efficiency	NNS
cord-014597-66vd2mdu	2178	3	in	in	IN
cord-014597-66vd2mdu	2178	4	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	2178	5	of	of	IN
cord-014597-66vd2mdu	2178	6	up	up	IN
cord-014597-66vd2mdu	2178	7	to	to	TO
cord-014597-66vd2mdu	2178	8	55	55	CD
cord-014597-66vd2mdu	2178	9	%	%	NN
cord-014597-66vd2mdu	2178	10	were	be	VBD
cord-014597-66vd2mdu	2178	11	comparable	comparable	JJ
cord-014597-66vd2mdu	2178	12	to	to	IN
cord-014597-66vd2mdu	2178	13	that	that	DT
cord-014597-66vd2mdu	2178	14	obtained	obtain	VBN
cord-014597-66vd2mdu	2178	15	in	in	IN
cord-014597-66vd2mdu	2178	16	a	a	DT
cord-014597-66vd2mdu	2178	17	simultaneous	simultaneous	JJ
cord-014597-66vd2mdu	2178	18	shaker	shaker	NN
cord-014597-66vd2mdu	2178	19	flask	flask	NN
cord-014597-66vd2mdu	2178	20	experiment	experiment	NN
cord-014597-66vd2mdu	2178	21	.	.	.
cord-014597-66vd2mdu	2179	1	Transfection	transfection	NN
cord-014597-66vd2mdu	2179	2	efficiencies	efficiency	NNS
cord-014597-66vd2mdu	2179	3	were	be	VBD
cord-014597-66vd2mdu	2179	4	lower	low	JJR
cord-014597-66vd2mdu	2179	5	compared	compare	VBN
cord-014597-66vd2mdu	2179	6	to	to	IN
cord-014597-66vd2mdu	2179	7	prior	prior	JJ
cord-014597-66vd2mdu	2179	8	experiments	experiment	NNS
cord-014597-66vd2mdu	2179	9	due	due	JJ
cord-014597-66vd2mdu	2179	10	to	to	IN
cord-014597-66vd2mdu	2179	11	controlled	control	VBN
cord-014597-66vd2mdu	2179	12	conditions	condition	NNS
cord-014597-66vd2mdu	2179	13	in	in	IN
cord-014597-66vd2mdu	2179	14	the	the	DT
cord-014597-66vd2mdu	2179	15	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2179	16	.	.	.
cord-014597-66vd2mdu	2180	1	Nonetheless	nonetheless	RB
cord-014597-66vd2mdu	2180	2	the	the	DT
cord-014597-66vd2mdu	2180	3	titer	titer	NN
cord-014597-66vd2mdu	2180	4	with	with	IN
cord-014597-66vd2mdu	2180	5	up	up	IN
cord-014597-66vd2mdu	2180	6	to	to	TO
cord-014597-66vd2mdu	2180	7	1×10	1×10	CD
cord-014597-66vd2mdu	2180	8	5	5	CD
cord-014597-66vd2mdu	2180	9	genomic	genomic	JJ
cord-014597-66vd2mdu	2180	10	copies	copy	NNS
cord-014597-66vd2mdu	2180	11	per	per	IN
cord-014597-66vd2mdu	2180	12	cell	cell	NN
cord-014597-66vd2mdu	2180	13	was	be	VBD
cord-014597-66vd2mdu	2180	14	elevated	elevate	VBN
cord-014597-66vd2mdu	2180	15	compared	compare	VBN
cord-014597-66vd2mdu	2180	16	to	to	IN
cord-014597-66vd2mdu	2180	17	that	that	DT
cord-014597-66vd2mdu	2180	18	of	of	IN
cord-014597-66vd2mdu	2180	19	shaker	shaker	NN
cord-014597-66vd2mdu	2180	20	flasks	flask	NNS
cord-014597-66vd2mdu	2180	21	.	.	.
cord-014597-66vd2mdu	2181	1	First	first	JJ
cord-014597-66vd2mdu	2181	2	experiments	experiment	NNS
cord-014597-66vd2mdu	2181	3	with	with	IN
cord-014597-66vd2mdu	2181	4	293-F	293-f	CD
cord-014597-66vd2mdu	2181	5	cells	cell	NNS
cord-014597-66vd2mdu	2181	6	in	in	IN
cord-014597-66vd2mdu	2181	7	HEK	HEK	NNP
cord-014597-66vd2mdu	2181	8	TF	TF	NNP
cord-014597-66vd2mdu	2181	9	medium	medium	NN
cord-014597-66vd2mdu	2181	10	showed	show	VBD
cord-014597-66vd2mdu	2181	11	promising	promising	JJ
cord-014597-66vd2mdu	2181	12	results	result	NNS
cord-014597-66vd2mdu	2181	13	of	of	IN
cord-014597-66vd2mdu	2181	14	transferring	transfer	VBG
cord-014597-66vd2mdu	2181	15	rAAV	raav	NN
cord-014597-66vd2mdu	2181	16	production	production	NN
cord-014597-66vd2mdu	2181	17	from	from	IN
cord-014597-66vd2mdu	2181	18	the	the	DT
cord-014597-66vd2mdu	2181	19	adherent	adherent	JJ
cord-014597-66vd2mdu	2181	20	system	system	NN
cord-014597-66vd2mdu	2181	21	to	to	IN
cord-014597-66vd2mdu	2181	22	suspension	suspension	NN
cord-014597-66vd2mdu	2181	23	.	.	.
cord-014597-66vd2mdu	2182	1	After	after	IN
cord-014597-66vd2mdu	2182	2	improvement	improvement	NN
cord-014597-66vd2mdu	2182	3	of	of	IN
cord-014597-66vd2mdu	2182	4	transfections	transfection	NNS
cord-014597-66vd2mdu	2182	5	by	by	IN
cord-014597-66vd2mdu	2182	6	the	the	DT
cord-014597-66vd2mdu	2182	7	adjustment	adjustment	NN
cord-014597-66vd2mdu	2182	8	of	of	IN
cord-014597-66vd2mdu	2182	9	DNA	dna	NN
cord-014597-66vd2mdu	2182	10	amounts	amount	NNS
cord-014597-66vd2mdu	2182	11	in	in	IN
cord-014597-66vd2mdu	2182	12	small	small	JJ
cord-014597-66vd2mdu	2182	13	scale	scale	NN
cord-014597-66vd2mdu	2182	14	experiments	experiment	NNS
cord-014597-66vd2mdu	2182	15	,	,	,
cord-014597-66vd2mdu	2182	16	AAV	AAV	NNP
cord-014597-66vd2mdu	2182	17	production	production	NN
cord-014597-66vd2mdu	2182	18	was	be	VBD
cord-014597-66vd2mdu	2182	19	analyzed	analyze	VBN
cord-014597-66vd2mdu	2182	20	in	in	IN
cord-014597-66vd2mdu	2182	21	shaker	shaker	NN
cord-014597-66vd2mdu	2182	22	flasks	flask	NNS
cord-014597-66vd2mdu	2182	23	.	.	.
cord-014597-66vd2mdu	2183	1	The	the	DT
cord-014597-66vd2mdu	2183	2	batch	batch	NN
cord-014597-66vd2mdu	2183	3	process	process	NN
cord-014597-66vd2mdu	2183	4	showed	show	VBD
cord-014597-66vd2mdu	2183	5	an	an	DT
cord-014597-66vd2mdu	2183	6	expected	expect	VBN
cord-014597-66vd2mdu	2183	7	increase	increase	NN
cord-014597-66vd2mdu	2183	8	in	in	IN
cord-014597-66vd2mdu	2183	9	cell	cell	NN
cord-014597-66vd2mdu	2183	10	density	density	NN
cord-014597-66vd2mdu	2183	11	with	with	IN
cord-014597-66vd2mdu	2183	12	low	low	JJ
cord-014597-66vd2mdu	2183	13	variability	variability	NN
cord-014597-66vd2mdu	2183	14	between	between	IN
cord-014597-66vd2mdu	2183	15	biological	biological	JJ
cord-014597-66vd2mdu	2183	16	replicates	replicate	NNS
cord-014597-66vd2mdu	2183	17	(	(	-LRB-
cord-014597-66vd2mdu	2183	18	Fig	Fig	NNP
cord-014597-66vd2mdu	2183	19	.	.	.
cord-014597-66vd2mdu	2183	20	1b	1b	CD
cord-014597-66vd2mdu	2183	21	)	)	-RRB-
cord-014597-66vd2mdu	2183	22	.	.	.
cord-014597-66vd2mdu	2184	1	The	the	DT
cord-014597-66vd2mdu	2184	2	genomic	genomic	JJ
cord-014597-66vd2mdu	2184	3	titer	titer	NN
cord-014597-66vd2mdu	2184	4	increased	increase	VBD
cord-014597-66vd2mdu	2184	5	according	accord	VBG
cord-014597-66vd2mdu	2184	6	to	to	IN
cord-014597-66vd2mdu	2184	7	the	the	DT
cord-014597-66vd2mdu	2184	8	viable	viable	JJ
cord-014597-66vd2mdu	2184	9	cell	cell	NN
cord-014597-66vd2mdu	2184	10	density	density	NN
cord-014597-66vd2mdu	2184	11	until	until	IN
cord-014597-66vd2mdu	2184	12	day	day	NN
cord-014597-66vd2mdu	2184	13	four	four	CD
cord-014597-66vd2mdu	2184	14	where	where	WRB
cord-014597-66vd2mdu	2184	15	a	a	DT
cord-014597-66vd2mdu	2184	16	sudden	sudden	JJ
cord-014597-66vd2mdu	2184	17	drop	drop	NN
cord-014597-66vd2mdu	2184	18	started	start	VBD
cord-014597-66vd2mdu	2184	19	.	.	.
cord-014597-66vd2mdu	2185	1	This	this	DT
cord-014597-66vd2mdu	2185	2	observation	observation	NN
cord-014597-66vd2mdu	2185	3	was	be	VBD
cord-014597-66vd2mdu	2185	4	made	make	VBN
cord-014597-66vd2mdu	2185	5	for	for	IN
cord-014597-66vd2mdu	2185	6	AAV	AAV	NNP
cord-014597-66vd2mdu	2185	7	productions	production	NNS
cord-014597-66vd2mdu	2185	8	in	in	IN
cord-014597-66vd2mdu	2185	9	HEK	HEK	NNP
cord-014597-66vd2mdu	2185	10	-	-	HYPH
cord-014597-66vd2mdu	2185	11	TF	TF	NNP
cord-014597-66vd2mdu	2185	12	,	,	,
cord-014597-66vd2mdu	2185	13	HEK	HEK	NNP
cord-014597-66vd2mdu	2185	14	-	-	HYPH
cord-014597-66vd2mdu	2185	15	GM	GM	NNP
cord-014597-66vd2mdu	2185	16	and	and	CC
cord-014597-66vd2mdu	2185	17	Freestyle	Freestyle	NNP
cord-014597-66vd2mdu	2185	18	F17	F17	NNP
cord-014597-66vd2mdu	2185	19	medium	medium	NN
cord-014597-66vd2mdu	2185	20	.	.	.
cord-014597-66vd2mdu	2186	1	For	for	IN
cord-014597-66vd2mdu	2186	2	optimal	optimal	JJ
cord-014597-66vd2mdu	2186	3	yields	yield	NNS
cord-014597-66vd2mdu	2186	4	,	,	,
cord-014597-66vd2mdu	2186	5	we	-PRON-	PRP
cord-014597-66vd2mdu	2186	6	assume	assume	VBP
cord-014597-66vd2mdu	2186	7	that	that	IN
cord-014597-66vd2mdu	2186	8	a	a	DT
cord-014597-66vd2mdu	2186	9	slight	slight	JJ
cord-014597-66vd2mdu	2186	10	decrease	decrease	NN
cord-014597-66vd2mdu	2186	11	in	in	IN
cord-014597-66vd2mdu	2186	12	viability	viability	NN
cord-014597-66vd2mdu	2186	13	marks	mark	VBZ
cord-014597-66vd2mdu	2186	14	the	the	DT
cord-014597-66vd2mdu	2186	15	point	point	NN
cord-014597-66vd2mdu	2186	16	in	in	IN
cord-014597-66vd2mdu	2186	17	time	time	NN
cord-014597-66vd2mdu	2186	18	for	for	IN
cord-014597-66vd2mdu	2186	19	harvest	harvest	NN
cord-014597-66vd2mdu	2186	20	.	.	.
cord-014597-66vd2mdu	2187	1	From	from	IN
cord-014597-66vd2mdu	2187	2	optimized	optimize	VBN
cord-014597-66vd2mdu	2187	3	protocols	protocol	NNS
cord-014597-66vd2mdu	2187	4	,	,	,
cord-014597-66vd2mdu	2187	5	a	a	DT
cord-014597-66vd2mdu	2187	6	batch	batch	NN
cord-014597-66vd2mdu	2187	7	process	process	NN
cord-014597-66vd2mdu	2187	8	in	in	IN
cord-014597-66vd2mdu	2187	9	a	a	DT
cord-014597-66vd2mdu	2187	10	2	2	CD
cord-014597-66vd2mdu	2187	11	l	l	NN
cord-014597-66vd2mdu	2187	12	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2187	13	was	be	VBD
cord-014597-66vd2mdu	2187	14	carried	carry	VBN
cord-014597-66vd2mdu	2187	15	out	out	RP
cord-014597-66vd2mdu	2187	16	.	.	.
cord-014597-66vd2mdu	2188	1	Interestingly	interestingly	RB
cord-014597-66vd2mdu	2188	2	the	the	DT
cord-014597-66vd2mdu	2188	3	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2188	4	cultivation	cultivation	NN
cord-014597-66vd2mdu	2188	5	resulted	result	VBD
cord-014597-66vd2mdu	2188	6	in	in	IN
cord-014597-66vd2mdu	2188	7	lower	low	JJR
cord-014597-66vd2mdu	2188	8	overall	overall	JJ
cord-014597-66vd2mdu	2188	9	viable	viable	JJ
cord-014597-66vd2mdu	2188	10	cell	cell	NN
cord-014597-66vd2mdu	2188	11	densities	density	NNS
cord-014597-66vd2mdu	2188	12	but	but	CC
cord-014597-66vd2mdu	2188	13	in	in	IN
cord-014597-66vd2mdu	2188	14	higher	high	JJR
cord-014597-66vd2mdu	2188	15	genomic	genomic	JJ
cord-014597-66vd2mdu	2188	16	copies	copy	NNS
cord-014597-66vd2mdu	2188	17	per	per	IN
cord-014597-66vd2mdu	2188	18	cell	cell	NN
cord-014597-66vd2mdu	2188	19	compared	compare	VBN
cord-014597-66vd2mdu	2188	20	to	to	IN
cord-014597-66vd2mdu	2188	21	shaker	shaker	NN
cord-014597-66vd2mdu	2188	22	flasks	flask	NNS
cord-014597-66vd2mdu	2188	23	(	(	-LRB-
cord-014597-66vd2mdu	2188	24	Fig	Fig	NNP
cord-014597-66vd2mdu	2188	25	.	.	.
cord-014597-66vd2mdu	2188	26	1c	1c	CD
cord-014597-66vd2mdu	2188	27	)	)	-RRB-
cord-014597-66vd2mdu	2188	28	.	.	.
cord-014597-66vd2mdu	2189	1	These	these	DT
cord-014597-66vd2mdu	2189	2	results	result	NNS
cord-014597-66vd2mdu	2189	3	are	be	VBP
cord-014597-66vd2mdu	2189	4	comparable	comparable	JJ
cord-014597-66vd2mdu	2189	5	to	to	IN
cord-014597-66vd2mdu	2189	6	already	already	RB
cord-014597-66vd2mdu	2189	7	published	publish	VBN
cord-014597-66vd2mdu	2189	8	data	datum	NNS
cord-014597-66vd2mdu	2189	9	for	for	IN
cord-014597-66vd2mdu	2189	10	suspension	suspension	NN
cord-014597-66vd2mdu	2189	11	cells	cell	NNS
cord-014597-66vd2mdu	2189	12	[	[	-LRB-
cord-014597-66vd2mdu	2189	13	2	2	CD
cord-014597-66vd2mdu	2189	14	]	]	-RRB-
cord-014597-66vd2mdu	2189	15	.	.	.
cord-014597-66vd2mdu	2190	1	Subsequent	subsequent	JJ
cord-014597-66vd2mdu	2190	2	optimization	optimization	NN
cord-014597-66vd2mdu	2190	3	of	of	IN
cord-014597-66vd2mdu	2190	4	the	the	DT
cord-014597-66vd2mdu	2190	5	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2190	6	protocol	protocol	NN
cord-014597-66vd2mdu	2190	7	will	will	MD
cord-014597-66vd2mdu	2190	8	lead	lead	VB
cord-014597-66vd2mdu	2190	9	to	to	IN
cord-014597-66vd2mdu	2190	10	further	further	JJ
cord-014597-66vd2mdu	2190	11	increase	increase	VB
cord-014597-66vd2mdu	2190	12	in	in	IN
cord-014597-66vd2mdu	2190	13	rAAV	raav	JJ
cord-014597-66vd2mdu	2190	14	yield	yield	NN
cord-014597-66vd2mdu	2190	15	.	.	.
cord-014597-66vd2mdu	2191	1	Genethon	Genethon	NNP
cord-014597-66vd2mdu	2191	2	and	and	CC
cord-014597-66vd2mdu	2191	3	Pall	Pall	NNP
cord-014597-66vd2mdu	2191	4	have	have	VBP
cord-014597-66vd2mdu	2191	5	collaborated	collaborate	VBN
cord-014597-66vd2mdu	2191	6	to	to	TO
cord-014597-66vd2mdu	2191	7	assess	assess	VB
cord-014597-66vd2mdu	2191	8	Pall	Pall	NNP
cord-014597-66vd2mdu	2191	9	's	's	POS
cord-014597-66vd2mdu	2191	10	single	single	JJ
cord-014597-66vd2mdu	2191	11	-	-	HYPH
cord-014597-66vd2mdu	2191	12	use	use	NN
cord-014597-66vd2mdu	2191	13	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2191	14	fixed	fix	VBN
cord-014597-66vd2mdu	2191	15	-	-	HYPH
cord-014597-66vd2mdu	2191	16	bed	bed	NN
cord-014597-66vd2mdu	2191	17	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2191	18	for	for	IN
cord-014597-66vd2mdu	2191	19	viral	viral	JJ
cord-014597-66vd2mdu	2191	20	vector	vector	NN
cord-014597-66vd2mdu	2191	21	production	production	NN
cord-014597-66vd2mdu	2191	22	.	.	.
cord-014597-66vd2mdu	2192	1	Clinical	clinical	JJ
cord-014597-66vd2mdu	2192	2	use	use	NN
cord-014597-66vd2mdu	2192	3	of	of	IN
cord-014597-66vd2mdu	2192	4	gene	gene	NN
cord-014597-66vd2mdu	2192	5	therapies	therapy	NNS
cord-014597-66vd2mdu	2192	6	to	to	TO
cord-014597-66vd2mdu	2192	7	treat	treat	VB
cord-014597-66vd2mdu	2192	8	formerly	formerly	RB
cord-014597-66vd2mdu	2192	9	incurable	incurable	JJ
cord-014597-66vd2mdu	2192	10	genetic	genetic	JJ
cord-014597-66vd2mdu	2192	11	diseases	disease	NNS
cord-014597-66vd2mdu	2192	12	is	be	VBZ
cord-014597-66vd2mdu	2192	13	advancing	advance	VBG
cord-014597-66vd2mdu	2192	14	rapidly	rapidly	RB
cord-014597-66vd2mdu	2192	15	.	.	.
cord-014597-66vd2mdu	2193	1	Viral	viral	JJ
cord-014597-66vd2mdu	2193	2	vectors	vector	NNS
cord-014597-66vd2mdu	2193	3	are	be	VBP
cord-014597-66vd2mdu	2193	4	an	an	DT
cord-014597-66vd2mdu	2193	5	important	important	JJ
cord-014597-66vd2mdu	2193	6	tool	tool	NN
cord-014597-66vd2mdu	2193	7	for	for	IN
cord-014597-66vd2mdu	2193	8	introducing	introduce	VBG
cord-014597-66vd2mdu	2193	9	genes	gene	NNS
cord-014597-66vd2mdu	2193	10	into	into	IN
cord-014597-66vd2mdu	2193	11	target	target	NN
cord-014597-66vd2mdu	2193	12	cells	cell	NNS
cord-014597-66vd2mdu	2193	13	.	.	.
cord-014597-66vd2mdu	2194	1	Many	many	JJ
cord-014597-66vd2mdu	2194	2	gene	gene	NN
cord-014597-66vd2mdu	2194	3	therapies	therapy	NNS
cord-014597-66vd2mdu	2194	4	have	have	VBP
cord-014597-66vd2mdu	2194	5	been	be	VBN
cord-014597-66vd2mdu	2194	6	developed	develop	VBN
cord-014597-66vd2mdu	2194	7	using	use	VBG
cord-014597-66vd2mdu	2194	8	adherent	adherent	JJ
cord-014597-66vd2mdu	2194	9	cells	cell	NNS
cord-014597-66vd2mdu	2194	10	in	in	IN
cord-014597-66vd2mdu	2194	11	2-dimensional	2-dimensional	JJ
cord-014597-66vd2mdu	2194	12	flatware	flatware	NN
cord-014597-66vd2mdu	2194	13	or	or	CC
cord-014597-66vd2mdu	2194	14	roller	roller	NN
cord-014597-66vd2mdu	2194	15	bottles	bottle	NNS
cord-014597-66vd2mdu	2194	16	but	but	CC
cord-014597-66vd2mdu	2194	17	using	use	VBG
cord-014597-66vd2mdu	2194	18	these	these	DT
cord-014597-66vd2mdu	2194	19	technologies	technology	NNS
cord-014597-66vd2mdu	2194	20	to	to	TO
cord-014597-66vd2mdu	2194	21	reach	reach	VB
cord-014597-66vd2mdu	2194	22	commercial	commercial	JJ
cord-014597-66vd2mdu	2194	23	-	-	HYPH
cord-014597-66vd2mdu	2194	24	scale	scale	NN
cord-014597-66vd2mdu	2194	25	production	production	NN
cord-014597-66vd2mdu	2194	26	represents	represent	VBZ
cord-014597-66vd2mdu	2194	27	a	a	DT
cord-014597-66vd2mdu	2194	28	significant	significant	JJ
cord-014597-66vd2mdu	2194	29	challenge	challenge	NN
cord-014597-66vd2mdu	2194	30	.	.	.
cord-014597-66vd2mdu	2195	1	The	the	DT
cord-014597-66vd2mdu	2195	2	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2195	3	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2195	4	enables	enable	VBZ
cord-014597-66vd2mdu	2195	5	large	large	JJ
cord-014597-66vd2mdu	2195	6	-	-	HYPH
cord-014597-66vd2mdu	2195	7	scale	scale	NN
cord-014597-66vd2mdu	2195	8	viral	viral	JJ
cord-014597-66vd2mdu	2195	9	vector	vector	NN
cord-014597-66vd2mdu	2195	10	production	production	NN
cord-014597-66vd2mdu	2195	11	by	by	IN
cord-014597-66vd2mdu	2195	12	providing	provide	VBG
cord-014597-66vd2mdu	2195	13	a	a	DT
cord-014597-66vd2mdu	2195	14	3-dimensional	3-dimensional	CD
cord-014597-66vd2mdu	2195	15	matrix	matrix	NN
cord-014597-66vd2mdu	2195	16	for	for	IN
cord-014597-66vd2mdu	2195	17	cell	cell	NN
cord-014597-66vd2mdu	2195	18	growth	growth	NN
cord-014597-66vd2mdu	2195	19	in	in	IN
cord-014597-66vd2mdu	2195	20	a	a	DT
cord-014597-66vd2mdu	2195	21	compact	compact	JJ
cord-014597-66vd2mdu	2195	22	configuration	configuration	NN
cord-014597-66vd2mdu	2195	23	(	(	-LRB-
cord-014597-66vd2mdu	2195	24	Fig	Fig	NNP
cord-014597-66vd2mdu	2195	25	.	.	.
cord-014597-66vd2mdu	2195	26	1	1	CD
cord-014597-66vd2mdu	2195	27	)	)	-RRB-
cord-014597-66vd2mdu	2195	28	.	.	.
cord-014597-66vd2mdu	2196	1	Up	up	IN
cord-014597-66vd2mdu	2196	2	to	to	TO
cord-014597-66vd2mdu	2196	3	500	500	CD
cord-014597-66vd2mdu	2196	4	m	m	CD
cord-014597-66vd2mdu	2196	5	2	2	CD
cord-014597-66vd2mdu	2196	6	of	of	IN
cord-014597-66vd2mdu	2196	7	surface	surface	NN
cord-014597-66vd2mdu	2196	8	area	area	NN
cord-014597-66vd2mdu	2196	9	is	be	VBZ
cord-014597-66vd2mdu	2196	10	available	available	JJ
cord-014597-66vd2mdu	2196	11	in	in	IN
cord-014597-66vd2mdu	2196	12	a	a	DT
cord-014597-66vd2mdu	2196	13	compact	compact	JJ
cord-014597-66vd2mdu	2196	14	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2196	15	measuring	measure	VBG
cord-014597-66vd2mdu	2196	16	88	88	CD
cord-014597-66vd2mdu	2196	17	mm	mm	NNS
cord-014597-66vd2mdu	2196	18	in	in	IN
cord-014597-66vd2mdu	2196	19	diameter	diameter	NN
cord-014597-66vd2mdu	2196	20	in	in	IN
cord-014597-66vd2mdu	2196	21	a	a	DT
cord-014597-66vd2mdu	2196	22	total	total	JJ
cord-014597-66vd2mdu	2196	23	volume	volume	NN
cord-014597-66vd2mdu	2196	24	of	of	IN
cord-014597-66vd2mdu	2196	25	75	75	CD
cord-014597-66vd2mdu	2196	26	L	l	NN
cord-014597-66vd2mdu	2196	27	with	with	IN
cord-014597-66vd2mdu	2196	28	pH	ph	NN
cord-014597-66vd2mdu	2196	29	,	,	,
cord-014597-66vd2mdu	2196	30	DO	do	VB
cord-014597-66vd2mdu	2196	31	and	and	CC
cord-014597-66vd2mdu	2196	32	temperature	temperature	NN
cord-014597-66vd2mdu	2196	33	control	control	NN
cord-014597-66vd2mdu	2196	34	.	.	.
cord-014597-66vd2mdu	2197	1	A	a	DT
cord-014597-66vd2mdu	2197	2	key	key	JJ
cord-014597-66vd2mdu	2197	3	feature	feature	NN
cord-014597-66vd2mdu	2197	4	of	of	IN
cord-014597-66vd2mdu	2197	5	the	the	DT
cord-014597-66vd2mdu	2197	6	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2197	7	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2197	8	is	be	VBZ
cord-014597-66vd2mdu	2197	9	that	that	IN
cord-014597-66vd2mdu	2197	10	it	-PRON-	PRP
cord-014597-66vd2mdu	2197	11	scales	scale	VBZ
cord-014597-66vd2mdu	2197	12	by	by	IN
cord-014597-66vd2mdu	2197	13	increasing	increase	VBG
cord-014597-66vd2mdu	2197	14	the	the	DT
cord-014597-66vd2mdu	2197	15	diameter	diameter	NN
cord-014597-66vd2mdu	2197	16	of	of	IN
cord-014597-66vd2mdu	2197	17	the	the	DT
cord-014597-66vd2mdu	2197	18	fixed	fix	VBN
cord-014597-66vd2mdu	2197	19	-	-	HYPH
cord-014597-66vd2mdu	2197	20	bed	bed	NN
cord-014597-66vd2mdu	2197	21	while	while	IN
cord-014597-66vd2mdu	2197	22	keeping	keep	VBG
cord-014597-66vd2mdu	2197	23	the	the	DT
cord-014597-66vd2mdu	2197	24	height	height	NN
cord-014597-66vd2mdu	2197	25	constant	constant	JJ
cord-014597-66vd2mdu	2197	26	with	with	IN
cord-014597-66vd2mdu	2197	27	no	no	DT
cord-014597-66vd2mdu	2197	28	change	change	NN
cord-014597-66vd2mdu	2197	29	in	in	IN
cord-014597-66vd2mdu	2197	30	aspect	aspect	NN
cord-014597-66vd2mdu	2197	31	ratios	ratio	NNS
cord-014597-66vd2mdu	2197	32	.	.	.
cord-014597-66vd2mdu	2198	1	The	the	DT
cord-014597-66vd2mdu	2198	2	height	height	NN
cord-014597-66vd2mdu	2198	3	of	of	IN
cord-014597-66vd2mdu	2198	4	the	the	DT
cord-014597-66vd2mdu	2198	5	fixed	fix	VBN
cord-014597-66vd2mdu	2198	6	-	-	HYPH
cord-014597-66vd2mdu	2198	7	bed	bed	NN
cord-014597-66vd2mdu	2198	8	can	can	MD
cord-014597-66vd2mdu	2198	9	be	be	VB
cord-014597-66vd2mdu	2198	10	varied	varied	JJ
cord-014597-66vd2mdu	2198	11	(	(	-LRB-
cord-014597-66vd2mdu	2198	12	2	2	CD
cord-014597-66vd2mdu	2198	13	,	,	,
cord-014597-66vd2mdu	2198	14	4	4	CD
cord-014597-66vd2mdu	2198	15	and	and	CC
cord-014597-66vd2mdu	2198	16	10	10	CD
cord-014597-66vd2mdu	2198	17	cm	cm	NNS
cord-014597-66vd2mdu	2198	18	)	)	-RRB-
cord-014597-66vd2mdu	2198	19	as	as	RB
cord-014597-66vd2mdu	2198	20	well	well	RB
cord-014597-66vd2mdu	2198	21	as	as	IN
cord-014597-66vd2mdu	2198	22	density	density	NN
cord-014597-66vd2mdu	2198	23	of	of	IN
cord-014597-66vd2mdu	2198	24	carrier	carrier	NN
cord-014597-66vd2mdu	2198	25	packing	packing	NN
cord-014597-66vd2mdu	2198	26	(	(	-LRB-
cord-014597-66vd2mdu	2198	27	96	96	CD
cord-014597-66vd2mdu	2198	28	gm	gm	NNP
cord-014597-66vd2mdu	2198	29	/	/	SYM
cord-014597-66vd2mdu	2198	30	L	L	NNP
cord-014597-66vd2mdu	2198	31	or	or	CC
cord-014597-66vd2mdu	2198	32	144	144	CD
cord-014597-66vd2mdu	2198	33	gm	gm	NNP
cord-014597-66vd2mdu	2198	34	/	/	SYM
cord-014597-66vd2mdu	2198	35	L	L	NNP
cord-014597-66vd2mdu	2198	36	)	)	-RRB-
cord-014597-66vd2mdu	2198	37	.	.	.
cord-014597-66vd2mdu	2199	1	The	the	DT
cord-014597-66vd2mdu	2199	2	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2199	3	system	system	NN
cord-014597-66vd2mdu	2199	4	comes	come	VBZ
cord-014597-66vd2mdu	2199	5	in	in	IN
cord-014597-66vd2mdu	2199	6	two	two	CD
cord-014597-66vd2mdu	2199	7	formats	format	NNS
cord-014597-66vd2mdu	2199	8	,	,	,
cord-014597-66vd2mdu	2199	9	the	the	DT
cord-014597-66vd2mdu	2199	10	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2199	11	Nano	Nano	NNP
cord-014597-66vd2mdu	2199	12	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2199	13	(	(	-LRB-
cord-014597-66vd2mdu	2199	14	0.53	0.53	CD
cord-014597-66vd2mdu	2199	15	-	-	SYM
cord-014597-66vd2mdu	2199	16	4.0	4.0	CD
cord-014597-66vd2mdu	2199	17	m	m	CD
cord-014597-66vd2mdu	2199	18	2	2	CD
cord-014597-66vd2mdu	2199	19	)	)	-RRB-
cord-014597-66vd2mdu	2199	20	and	and	CC
cord-014597-66vd2mdu	2199	21	the	the	DT
cord-014597-66vd2mdu	2199	22	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2199	23	500	500	CD
cord-014597-66vd2mdu	2199	24	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2199	25	(	(	-LRB-
cord-014597-66vd2mdu	2199	26	66	66	CD
cord-014597-66vd2mdu	2199	27	-	-	SYM
cord-014597-66vd2mdu	2199	28	500	500	CD
cord-014597-66vd2mdu	2199	29	m	m	CD
cord-014597-66vd2mdu	2199	30	2	2	CD
cord-014597-66vd2mdu	2199	31	)	)	-RRB-
cord-014597-66vd2mdu	2199	32	.	.	.
cord-014597-66vd2mdu	2200	1	Processes	process	NNS
cord-014597-66vd2mdu	2200	2	developed	develop	VBN
cord-014597-66vd2mdu	2200	3	in	in	IN
cord-014597-66vd2mdu	2200	4	the	the	DT
cord-014597-66vd2mdu	2200	5	bench	bench	NN
cord-014597-66vd2mdu	2200	6	top	top	NN
cord-014597-66vd2mdu	2200	7	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2200	8	Nano	Nano	NNP
cord-014597-66vd2mdu	2200	9	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2200	10	can	can	MD
cord-014597-66vd2mdu	2200	11	be	be	VB
cord-014597-66vd2mdu	2200	12	directly	directly	RB
cord-014597-66vd2mdu	2200	13	transferred	transfer	VBN
cord-014597-66vd2mdu	2200	14	to	to	IN
cord-014597-66vd2mdu	2200	15	the	the	DT
cord-014597-66vd2mdu	2200	16	corresponding	corresponding	JJ
cord-014597-66vd2mdu	2200	17	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2200	18	500	500	CD
cord-014597-66vd2mdu	2200	19	system	system	NN
cord-014597-66vd2mdu	2200	20	.	.	.
cord-014597-66vd2mdu	2201	1	The	the	DT
cord-014597-66vd2mdu	2201	2	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2201	3	Nano	Nano	NNP
cord-014597-66vd2mdu	2201	4	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2201	5	enables	enable	VBZ
cord-014597-66vd2mdu	2201	6	an	an	DT
cord-014597-66vd2mdu	2201	7	efficient	efficient	JJ
cord-014597-66vd2mdu	2201	8	platform	platform	NN
cord-014597-66vd2mdu	2201	9	for	for	IN
cord-014597-66vd2mdu	2201	10	process	process	NN
cord-014597-66vd2mdu	2201	11	optimization	optimization	NN
cord-014597-66vd2mdu	2201	12	.	.	.
cord-014597-66vd2mdu	2201	13	 	 	_SP
cord-014597-66vd2mdu	2202	1	The	the	DT
cord-014597-66vd2mdu	2202	2	Genethon	Genethon	NNP
cord-014597-66vd2mdu	2203	1	rAAV-8	raav-8	LS
cord-014597-66vd2mdu	2203	2	process	process	NN
cord-014597-66vd2mdu	2203	3	was	be	VBD
cord-014597-66vd2mdu	2203	4	transferred	transfer	VBN
cord-014597-66vd2mdu	2203	5	to	to	IN
cord-014597-66vd2mdu	2203	6	an	an	DT
cord-014597-66vd2mdu	2203	7	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2203	8	Nano	Nano	NNP
cord-014597-66vd2mdu	2203	9	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2203	10	0.8	0.8	CD
cord-014597-66vd2mdu	2203	11	m	m	CD
cord-014597-66vd2mdu	2203	12	2	2	CD
cord-014597-66vd2mdu	2203	13	(	(	-LRB-
cord-014597-66vd2mdu	2203	14	2	2	CD
cord-014597-66vd2mdu	2203	15	cm	cm	NN
cord-014597-66vd2mdu	2203	16	bed	bed	NN
cord-014597-66vd2mdu	2203	17	height	height	NN
cord-014597-66vd2mdu	2203	18	,	,	,
cord-014597-66vd2mdu	2203	19	144	144	CD
cord-014597-66vd2mdu	2203	20	gm	gm	NNP
cord-014597-66vd2mdu	2203	21	/	/	SYM
cord-014597-66vd2mdu	2203	22	L	l	NN
cord-014597-66vd2mdu	2203	23	density	density	NN
cord-014597-66vd2mdu	2203	24	)	)	-RRB-
cord-014597-66vd2mdu	2203	25	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2203	26	using	use	VBG
cord-014597-66vd2mdu	2203	27	FreeStyle	FreeStyle	NNP
cord-014597-66vd2mdu	2203	28	media	medium	NNS
cord-014597-66vd2mdu	2203	29	.	.	.
cord-014597-66vd2mdu	2204	1	The	the	DT
cord-014597-66vd2mdu	2204	2	initial	initial	JJ
cord-014597-66vd2mdu	2204	3	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2204	4	Nano	Nano	NNP
cord-014597-66vd2mdu	2204	5	process	process	NN
cord-014597-66vd2mdu	2204	6	was	be	VBD
cord-014597-66vd2mdu	2204	7	established	establish	VBN
cord-014597-66vd2mdu	2204	8	as	as	IN
cord-014597-66vd2mdu	2204	9	(	(	-LRB-
cord-014597-66vd2mdu	2204	10	1	1	CD
cord-014597-66vd2mdu	2204	11	)	)	-RRB-
cord-014597-66vd2mdu	2204	12	seed	seed	NN
cord-014597-66vd2mdu	2204	13	on	on	IN
cord-014597-66vd2mdu	2204	14	Day	day	NN
cord-014597-66vd2mdu	2204	15	1	1	CD
cord-014597-66vd2mdu	2204	16	,	,	,
cord-014597-66vd2mdu	2204	17	(	(	-LRB-
cord-014597-66vd2mdu	2204	18	2	2	LS
cord-014597-66vd2mdu	2204	19	)	)	-RRB-
cord-014597-66vd2mdu	2204	20	transfect	transfect	VB
cord-014597-66vd2mdu	2204	21	at	at	IN
cord-014597-66vd2mdu	2204	22	Day	day	NN
cord-014597-66vd2mdu	2204	23	5	5	CD
cord-014597-66vd2mdu	2204	24	,	,	,
cord-014597-66vd2mdu	2204	25	(	(	-LRB-
cord-014597-66vd2mdu	2204	26	3	3	LS
cord-014597-66vd2mdu	2204	27	)	)	-RRB-
cord-014597-66vd2mdu	2204	28	harvest	harvest	NN
cord-014597-66vd2mdu	2204	29	at	at	IN
cord-014597-66vd2mdu	2204	30	Day	day	NN
cord-014597-66vd2mdu	2204	31	8	8	CD
cord-014597-66vd2mdu	2204	32	and	and	CC
cord-014597-66vd2mdu	2204	33	yielded	yield	VBD
cord-014597-66vd2mdu	2204	34	<	<	NNP
cord-014597-66vd2mdu	2204	35	1x	1x	CD
cord-014597-66vd2mdu	2204	36	10	10	CD
cord-014597-66vd2mdu	2204	37	9	9	CD
cord-014597-66vd2mdu	2204	38	vp	vp	NNP
cord-014597-66vd2mdu	2204	39	/	/	SYM
cord-014597-66vd2mdu	2204	40	cm	cm	NNP
cord-014597-66vd2mdu	2204	41	2	2	CD
cord-014597-66vd2mdu	2204	42	(	(	-LRB-
cord-014597-66vd2mdu	2204	43	n=3	n=3	NN
cord-014597-66vd2mdu	2204	44	)	)	-RRB-
cord-014597-66vd2mdu	2204	45	.	.	.
cord-014597-66vd2mdu	2205	1	Media	Media	NNP
cord-014597-66vd2mdu	2205	2	exchange	exchange	NN
cord-014597-66vd2mdu	2205	3	,	,	,
cord-014597-66vd2mdu	2205	4	cell	cell	NN
cord-014597-66vd2mdu	2205	5	density	density	NN
cord-014597-66vd2mdu	2205	6	at	at	IN
cord-014597-66vd2mdu	2205	7	transfection	transfection	NN
cord-014597-66vd2mdu	2205	8	,	,	,
cord-014597-66vd2mdu	2205	9	pDNA	pdna	JJ
cord-014597-66vd2mdu	2205	10	/	/	SYM
cord-014597-66vd2mdu	2205	11	cell	cell	NN
cord-014597-66vd2mdu	2205	12	ratio	ratio	NN
cord-014597-66vd2mdu	2205	13	,	,	,
cord-014597-66vd2mdu	2205	14	and	and	CC
cord-014597-66vd2mdu	2205	15	lysis	lysis	NN
cord-014597-66vd2mdu	2205	16	method	method	NN
cord-014597-66vd2mdu	2205	17	were	be	VBD
cord-014597-66vd2mdu	2205	18	then	then	RB
cord-014597-66vd2mdu	2205	19	changed	change	VBN
cord-014597-66vd2mdu	2205	20	to	to	TO
cord-014597-66vd2mdu	2205	21	determine	determine	VB
cord-014597-66vd2mdu	2205	22	the	the	DT
cord-014597-66vd2mdu	2205	23	effect	effect	NN
cord-014597-66vd2mdu	2205	24	on	on	IN
cord-014597-66vd2mdu	2205	25	productivity	productivity	NN
cord-014597-66vd2mdu	2205	26	.	.	.
cord-014597-66vd2mdu	2206	1	The	the	DT
cord-014597-66vd2mdu	2206	2	modified	modified	JJ
cord-014597-66vd2mdu	2206	3	process	process	NN
cord-014597-66vd2mdu	2206	4	was	be	VBD
cord-014597-66vd2mdu	2206	5	then	then	RB
cord-014597-66vd2mdu	2206	6	scaled	scale	VBN
cord-014597-66vd2mdu	2206	7	from	from	IN
cord-014597-66vd2mdu	2206	8	0.8	0.8	CD
cord-014597-66vd2mdu	2206	9	m	m	CD
cord-014597-66vd2mdu	2206	10	2	2	CD
cord-014597-66vd2mdu	2206	11	to	to	IN
cord-014597-66vd2mdu	2206	12	4.0	4.0	CD
cord-014597-66vd2mdu	2206	13	m	m	CD
cord-014597-66vd2mdu	2206	14	2	2	CD
cord-014597-66vd2mdu	2206	15	(	(	-LRB-
cord-014597-66vd2mdu	2206	16	10	10	CD
cord-014597-66vd2mdu	2206	17	cm	cm	NN
cord-014597-66vd2mdu	2206	18	bed	bed	NN
cord-014597-66vd2mdu	2206	19	height	height	NN
cord-014597-66vd2mdu	2206	20	,	,	,
cord-014597-66vd2mdu	2206	21	144	144	CD
cord-014597-66vd2mdu	2206	22	gm	gm	NNP
cord-014597-66vd2mdu	2206	23	/	/	SYM
cord-014597-66vd2mdu	2206	24	L	l	NN
cord-014597-66vd2mdu	2206	25	density	density	NN
cord-014597-66vd2mdu	2206	26	)	)	-RRB-
cord-014597-66vd2mdu	2207	1	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2207	2	Nano	Nano	NNP
cord-014597-66vd2mdu	2207	3	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2207	4	.	.	.
cord-014597-66vd2mdu	2208	1	-Media	-Media	NNP
cord-014597-66vd2mdu	2209	1	:	:	:
cord-014597-66vd2mdu	2209	2	A	a	DT
cord-014597-66vd2mdu	2209	3	media	media	NN
cord-014597-66vd2mdu	2209	4	exchange	exchange	NN
cord-014597-66vd2mdu	2209	5	at	at	IN
cord-014597-66vd2mdu	2209	6	5	5	CD
cord-014597-66vd2mdu	2209	7	hours	hour	NNS
cord-014597-66vd2mdu	2209	8	post	post	NN
cord-014597-66vd2mdu	2209	9	transfection	transfection	NN
cord-014597-66vd2mdu	2209	10	with	with	IN
cord-014597-66vd2mdu	2209	11	DMEM	DMEM	NNP
cord-014597-66vd2mdu	2209	12	substituted	substitute	VBN
cord-014597-66vd2mdu	2209	13	for	for	IN
cord-014597-66vd2mdu	2209	14	FreeStyle	FreeStyle	NNP
cord-014597-66vd2mdu	2209	15	medium	medium	NN
cord-014597-66vd2mdu	2209	16	resulted	result	VBD
cord-014597-66vd2mdu	2209	17	in	in	IN
cord-014597-66vd2mdu	2209	18	an	an	DT
cord-014597-66vd2mdu	2209	19	8x	8x	CD
cord-014597-66vd2mdu	2209	20	increase	increase	NN
cord-014597-66vd2mdu	2209	21	in	in	IN
cord-014597-66vd2mdu	2209	22	specific	specific	JJ
cord-014597-66vd2mdu	2209	23	productivity	productivity	NN
cord-014597-66vd2mdu	2209	24	.	.	.
cord-014597-66vd2mdu	2210	1	1	1	LS
cord-014597-66vd2mdu	2211	1	(	(	-LRB-
cord-014597-66vd2mdu	2211	2	abstract	abstract	NNP
cord-014597-66vd2mdu	2211	3	P-349	P-349	NNP
cord-014597-66vd2mdu	2211	4	)	)	-RRB-
cord-014597-66vd2mdu	2211	5	.	.	.
cord-014597-66vd2mdu	2212	1	a	a	DT
cord-014597-66vd2mdu	2212	2	Schematic	schematic	JJ
cord-014597-66vd2mdu	2212	3	overview	overview	NN
cord-014597-66vd2mdu	2212	4	of	of	IN
cord-014597-66vd2mdu	2212	5	rAAV	raav	NN
cord-014597-66vd2mdu	2212	6	production	production	NN
cord-014597-66vd2mdu	2212	7	in	in	IN
cord-014597-66vd2mdu	2212	8	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2212	9	cells	cell	NNS
cord-014597-66vd2mdu	2212	10	with	with	IN
cord-014597-66vd2mdu	2212	11	triple	triple	JJ
cord-014597-66vd2mdu	2212	12	-	-	HYPH
cord-014597-66vd2mdu	2212	13	transfection	transfection	NN
cord-014597-66vd2mdu	2212	14	system	system	NN
cord-014597-66vd2mdu	2212	15	.	.	.
cord-014597-66vd2mdu	2213	1	b	b	LS
cord-014597-66vd2mdu	2213	2	Viable	viable	JJ
cord-014597-66vd2mdu	2213	3	cell	cell	NN
cord-014597-66vd2mdu	2213	4	densities	density	NNS
cord-014597-66vd2mdu	2213	5	(	(	-LRB-
cord-014597-66vd2mdu	2213	6	VCD	VCD	NNP
cord-014597-66vd2mdu	2213	7	)	)	-RRB-
cord-014597-66vd2mdu	2213	8	,	,	,
cord-014597-66vd2mdu	2213	9	viabilities	viability	NNS
cord-014597-66vd2mdu	2213	10	and	and	CC
cord-014597-66vd2mdu	2213	11	genomic	genomic	JJ
cord-014597-66vd2mdu	2213	12	copies	copy	NNS
cord-014597-66vd2mdu	2213	13	per	per	IN
cord-014597-66vd2mdu	2213	14	ml	ml	NN
cord-014597-66vd2mdu	2213	15	(	(	-LRB-
cord-014597-66vd2mdu	2213	16	GC	GC	NNP
cord-014597-66vd2mdu	2213	17	)	)	-RRB-
cord-014597-66vd2mdu	2213	18	of	of	IN
cord-014597-66vd2mdu	2213	19	a	a	DT
cord-014597-66vd2mdu	2213	20	rAAV	raav	JJ
cord-014597-66vd2mdu	2213	21	production	production	NN
cord-014597-66vd2mdu	2213	22	with	with	IN
cord-014597-66vd2mdu	2213	23	293-F	293-f	CD
cord-014597-66vd2mdu	2213	24	batch	batch	NN
cord-014597-66vd2mdu	2213	25	cultivations	cultivation	NNS
cord-014597-66vd2mdu	2213	26	in	in	IN
cord-014597-66vd2mdu	2213	27	shaker	shaker	NN
cord-014597-66vd2mdu	2213	28	flasks	flask	NNS
cord-014597-66vd2mdu	2213	29	.	.	.
cord-014597-66vd2mdu	2214	1	Genomic	genomic	JJ
cord-014597-66vd2mdu	2214	2	copies	copy	NNS
cord-014597-66vd2mdu	2214	3	per	per	IN
cord-014597-66vd2mdu	2214	4	ml	ml	NNS
cord-014597-66vd2mdu	2214	5	refer	refer	NN
cord-014597-66vd2mdu	2214	6	to	to	IN
cord-014597-66vd2mdu	2214	7	the	the	DT
cord-014597-66vd2mdu	2214	8	titer	titer	NN
cord-014597-66vd2mdu	2214	9	determined	determine	VBN
cord-014597-66vd2mdu	2214	10	in	in	IN
cord-014597-66vd2mdu	2214	11	1	1	CD
cord-014597-66vd2mdu	2214	12	ml	ml	NN
cord-014597-66vd2mdu	2214	13	culture	culture	NN
cord-014597-66vd2mdu	2214	14	volume	volume	NN
cord-014597-66vd2mdu	2214	15	.	.	.
cord-014597-66vd2mdu	2215	1	Error	error	NN
cord-014597-66vd2mdu	2215	2	bars	bar	NNS
cord-014597-66vd2mdu	2215	3	represent	represent	VBP
cord-014597-66vd2mdu	2215	4	biological	biological	JJ
cord-014597-66vd2mdu	2215	5	and	and	CC
cord-014597-66vd2mdu	2215	6	technical	technical	JJ
cord-014597-66vd2mdu	2215	7	duplicate	duplicate	JJ
cord-014597-66vd2mdu	2215	8	measurements	measurement	NNS
cord-014597-66vd2mdu	2215	9	of	of	IN
cord-014597-66vd2mdu	2215	10	samples	sample	NNS
cord-014597-66vd2mdu	2215	11	.	.	.
cord-014597-66vd2mdu	2216	1	c	c	LS
cord-014597-66vd2mdu	2216	2	Viable	viable	JJ
cord-014597-66vd2mdu	2216	3	cell	cell	NN
cord-014597-66vd2mdu	2216	4	densities	density	NNS
cord-014597-66vd2mdu	2216	5	and	and	CC
cord-014597-66vd2mdu	2216	6	genomic	genomic	JJ
cord-014597-66vd2mdu	2216	7	copies	copy	NNS
cord-014597-66vd2mdu	2216	8	per	per	IN
cord-014597-66vd2mdu	2216	9	cell	cell	NN
cord-014597-66vd2mdu	2216	10	of	of	IN
cord-014597-66vd2mdu	2216	11	a	a	DT
cord-014597-66vd2mdu	2216	12	rAAV	raav	NN
cord-014597-66vd2mdu	2216	13	production	production	NN
cord-014597-66vd2mdu	2216	14	with	with	IN
cord-014597-66vd2mdu	2216	15	293-F	293-f	CD
cord-014597-66vd2mdu	2216	16	batch	batch	NN
cord-014597-66vd2mdu	2216	17	cultivation	cultivation	NN
cord-014597-66vd2mdu	2216	18	in	in	IN
cord-014597-66vd2mdu	2216	19	a	a	DT
cord-014597-66vd2mdu	2216	20	2	2	CD
cord-014597-66vd2mdu	2216	21	l	l	NN
cord-014597-66vd2mdu	2216	22	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2216	23	.	.	.
cord-014597-66vd2mdu	2217	1	For	for	IN
cord-014597-66vd2mdu	2217	2	reasons	reason	NNS
cord-014597-66vd2mdu	2217	3	of	of	IN
cord-014597-66vd2mdu	2217	4	comparability	comparability	NN
cord-014597-66vd2mdu	2217	5	between	between	IN
cord-014597-66vd2mdu	2217	6	shaker	shaker	NN
cord-014597-66vd2mdu	2217	7	and	and	CC
cord-014597-66vd2mdu	2217	8	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2217	9	data	datum	NNS
cord-014597-66vd2mdu	2217	10	genomic	genomic	JJ
cord-014597-66vd2mdu	2217	11	copies	copy	NNS
cord-014597-66vd2mdu	2217	12	are	be	VBP
cord-014597-66vd2mdu	2217	13	given	give	VBN
cord-014597-66vd2mdu	2217	14	per	per	IN
cord-014597-66vd2mdu	2217	15	cell	cell	NN
cord-014597-66vd2mdu	2217	16	.	.	.
cord-014597-66vd2mdu	2218	1	Error	error	NN
cord-014597-66vd2mdu	2218	2	bars	bar	NNS
cord-014597-66vd2mdu	2218	3	represent	represent	VBP
cord-014597-66vd2mdu	2218	4	technical	technical	JJ
cord-014597-66vd2mdu	2218	5	duplicate	duplicate	JJ
cord-014597-66vd2mdu	2218	6	measurements	measurement	NNS
cord-014597-66vd2mdu	2218	7	of	of	IN
cord-014597-66vd2mdu	2218	8	samples	sample	NNS
cord-014597-66vd2mdu	2218	9	-Cell	-Cell	JJR
cord-014597-66vd2mdu	2218	10	density	density	NN
cord-014597-66vd2mdu	2218	11	at	at	IN
cord-014597-66vd2mdu	2218	12	transfection	transfection	NN
cord-014597-66vd2mdu	2218	13	:	:	:
cord-014597-66vd2mdu	2219	1	Cells	cell	NNS
cord-014597-66vd2mdu	2219	2	were	be	VBD
cord-014597-66vd2mdu	2219	3	seeded	seed	VBN
cord-014597-66vd2mdu	2219	4	at	at	IN
cord-014597-66vd2mdu	2219	5	6,000	6,000	CD
cord-014597-66vd2mdu	2219	6	cells	cell	NNS
cord-014597-66vd2mdu	2219	7	/	/	SYM
cord-014597-66vd2mdu	2219	8	cm	cm	NN
cord-014597-66vd2mdu	2219	9	2	2	CD
cord-014597-66vd2mdu	2219	10	and	and	CC
cord-014597-66vd2mdu	2219	11	reached	reach	VBD
cord-014597-66vd2mdu	2219	12	200,000	200,000	CD
cord-014597-66vd2mdu	2219	13	cells	cell	NNS
cord-014597-66vd2mdu	2219	14	/	/	SYM
cord-014597-66vd2mdu	2219	15	cm	cm	NN
cord-014597-66vd2mdu	2219	16	2	2	CD
cord-014597-66vd2mdu	2219	17	at	at	IN
cord-014597-66vd2mdu	2219	18	Day	day	NN
cord-014597-66vd2mdu	2219	19	5	5	CD
cord-014597-66vd2mdu	2219	20	which	which	WDT
cord-014597-66vd2mdu	2219	21	was	be	VBD
cord-014597-66vd2mdu	2219	22	determined	determine	VBN
cord-014597-66vd2mdu	2219	23	to	to	TO
cord-014597-66vd2mdu	2219	24	be	be	VB
cord-014597-66vd2mdu	2219	25	the	the	DT
cord-014597-66vd2mdu	2219	26	optimal	optimal	JJ
cord-014597-66vd2mdu	2219	27	cell	cell	NN
cord-014597-66vd2mdu	2219	28	density	density	NN
cord-014597-66vd2mdu	2219	29	for	for	IN
cord-014597-66vd2mdu	2219	30	transfection	transfection	NN
cord-014597-66vd2mdu	2219	31	.	.	.
cord-014597-66vd2mdu	2220	1	-pDNA	-pDNA	.
cord-014597-66vd2mdu	2221	1	/cell	/cell	NNP
cord-014597-66vd2mdu	2221	2	ratio	ratio	NN
cord-014597-66vd2mdu	2221	3	:	:	:
cord-014597-66vd2mdu	2222	1	Reducing	reduce	VBG
cord-014597-66vd2mdu	2222	2	pDNA	pdna	RB
cord-014597-66vd2mdu	2222	3	by	by	IN
cord-014597-66vd2mdu	2222	4	50	50	CD
cord-014597-66vd2mdu	2222	5	%	%	NN
cord-014597-66vd2mdu	2222	6	had	have	VBD
cord-014597-66vd2mdu	2222	7	no	no	DT
cord-014597-66vd2mdu	2222	8	significant	significant	JJ
cord-014597-66vd2mdu	2222	9	effect	effect	NN
cord-014597-66vd2mdu	2222	10	on	on	IN
cord-014597-66vd2mdu	2222	11	productivity	productivity	NN
cord-014597-66vd2mdu	2222	12	.	.	.
cord-014597-66vd2mdu	2223	1	-Lysis	-lysis	NN
cord-014597-66vd2mdu	2224	1	:	:	:
cord-014597-66vd2mdu	2224	2	Use	Use	NNP
cord-014597-66vd2mdu	2224	3	of	of	IN
cord-014597-66vd2mdu	2224	4	Trion	Trion	NNP
cord-014597-66vd2mdu	2224	5	x-100	x-100	NNS
cord-014597-66vd2mdu	2224	6	at	at	IN
cord-014597-66vd2mdu	2224	7	0.5	0.5	CD
cord-014597-66vd2mdu	2224	8	%	%	NN
cord-014597-66vd2mdu	2224	9	with	with	IN
cord-014597-66vd2mdu	2224	10	100	100	CD
cord-014597-66vd2mdu	2224	11	mM	mm	CD
cord-014597-66vd2mdu	2224	12	NaCl	nacl	NN
cord-014597-66vd2mdu	2224	13	at	at	IN
cord-014597-66vd2mdu	2224	14	pH	pH	NNP
cord-014597-66vd2mdu	2224	15	8	8	CD
cord-014597-66vd2mdu	2224	16	resulted	result	VBD
cord-014597-66vd2mdu	2224	17	in	in	IN
cord-014597-66vd2mdu	2224	18	>	>	XX
cord-014597-66vd2mdu	2224	19	100	100	CD
cord-014597-66vd2mdu	2224	20	%	%	NN
cord-014597-66vd2mdu	2224	21	virus	virus	NN
cord-014597-66vd2mdu	2224	22	recovery	recovery	NN
cord-014597-66vd2mdu	2224	23	compared	compare	VBN
cord-014597-66vd2mdu	2224	24	to	to	IN
cord-014597-66vd2mdu	2224	25	sampled	sample	VBN
cord-014597-66vd2mdu	2224	26	carriers	carrier	NNS
cord-014597-66vd2mdu	2224	27	.	.	.
cord-014597-66vd2mdu	2225	1	-Scaling	-scale	VBG
cord-014597-66vd2mdu	2225	2	:	:	:
cord-014597-66vd2mdu	2225	3	Specific	specific	JJ
cord-014597-66vd2mdu	2225	4	productivity	productivity	NN
cord-014597-66vd2mdu	2225	5	was	be	VBD
cord-014597-66vd2mdu	2225	6	maintained	maintain	VBN
cord-014597-66vd2mdu	2225	7	as	as	IN
cord-014597-66vd2mdu	2225	8	the	the	DT
cord-014597-66vd2mdu	2225	9	system	system	NN
cord-014597-66vd2mdu	2225	10	was	be	VBD
cord-014597-66vd2mdu	2225	11	scaled	scale	VBN
cord-014597-66vd2mdu	2225	12	from	from	IN
cord-014597-66vd2mdu	2225	13	0.8	0.8	CD
cord-014597-66vd2mdu	2225	14	m	m	CD
cord-014597-66vd2mdu	2225	15	2	2	CD
cord-014597-66vd2mdu	2225	16	to	to	IN
cord-014597-66vd2mdu	2225	17	4.0	4.0	CD
cord-014597-66vd2mdu	2225	18	m	m	CD
cord-014597-66vd2mdu	2225	19	2	2	CD
cord-014597-66vd2mdu	2225	20	.	.	.
cord-014597-66vd2mdu	2226	1	-Overall	-overall	UH
cord-014597-66vd2mdu	2226	2	,	,	,
cord-014597-66vd2mdu	2226	3	an	an	DT
cord-014597-66vd2mdu	2226	4	average	average	JJ
cord-014597-66vd2mdu	2226	5	yield	yield	NN
cord-014597-66vd2mdu	2226	6	of	of	IN
cord-014597-66vd2mdu	2226	7	4x10	4x10	CD
cord-014597-66vd2mdu	2226	8	13	13	CD
cord-014597-66vd2mdu	2226	9	VG	VG	NNP
cord-014597-66vd2mdu	2226	10	/	/	SYM
cord-014597-66vd2mdu	2226	11	m	m	NN
cord-014597-66vd2mdu	2226	12	2	2	CD
cord-014597-66vd2mdu	2226	13	was	be	VBD
cord-014597-66vd2mdu	2226	14	achieved	achieve	VBN
cord-014597-66vd2mdu	2226	15	.	.	.
cord-014597-66vd2mdu	2227	1	The	the	DT
cord-014597-66vd2mdu	2227	2	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2227	3	technology	technology	NN
cord-014597-66vd2mdu	2227	4	is	be	VBZ
cord-014597-66vd2mdu	2227	5	being	be	VBG
cord-014597-66vd2mdu	2227	6	adopted	adopt	VBN
cord-014597-66vd2mdu	2227	7	widely	widely	RB
cord-014597-66vd2mdu	2227	8	for	for	IN
cord-014597-66vd2mdu	2227	9	viral	viral	JJ
cord-014597-66vd2mdu	2227	10	vector	vector	NN
cord-014597-66vd2mdu	2227	11	production	production	NN
cord-014597-66vd2mdu	2227	12	.	.	.
cord-014597-66vd2mdu	2228	1	Transferring	transfer	VBG
cord-014597-66vd2mdu	2228	2	a	a	DT
cord-014597-66vd2mdu	2228	3	process	process	NN
cord-014597-66vd2mdu	2228	4	to	to	IN
cord-014597-66vd2mdu	2228	5	the	the	DT
cord-014597-66vd2mdu	2228	6	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2228	7	Nano	Nano	NNP
cord-014597-66vd2mdu	2228	8	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2228	9	can	can	MD
cord-014597-66vd2mdu	2228	10	be	be	VB
cord-014597-66vd2mdu	2228	11	easily	easily	RB
cord-014597-66vd2mdu	2228	12	achieved	achieve	VBN
cord-014597-66vd2mdu	2228	13	and	and	CC
cord-014597-66vd2mdu	2228	14	once	once	RB
cord-014597-66vd2mdu	2228	15	in	in	IN
cord-014597-66vd2mdu	2228	16	place	place	NN
cord-014597-66vd2mdu	2228	17	can	can	MD
cord-014597-66vd2mdu	2228	18	be	be	VB
cord-014597-66vd2mdu	2228	19	optimized	optimize	VBN
cord-014597-66vd2mdu	2228	20	to	to	TO
cord-014597-66vd2mdu	2228	21	provide	provide	VB
cord-014597-66vd2mdu	2228	22	significant	significant	JJ
cord-014597-66vd2mdu	2228	23	productivity	productivity	NN
cord-014597-66vd2mdu	2228	24	increases	increase	NNS
cord-014597-66vd2mdu	2228	25	and	and	CC
cord-014597-66vd2mdu	2228	26	cost	cost	NN
cord-014597-66vd2mdu	2228	27	savings	saving	NNS
cord-014597-66vd2mdu	2228	28	such	such	JJ
cord-014597-66vd2mdu	2228	29	as	as	IN
cord-014597-66vd2mdu	2228	30	reduced	reduce	VBN
cord-014597-66vd2mdu	2228	31	pDNA	pdna	RB
cord-014597-66vd2mdu	2228	32	.	.	.
cord-014597-66vd2mdu	2229	1	The	the	DT
cord-014597-66vd2mdu	2229	2	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2229	3	Nano	Nano	NNP
cord-014597-66vd2mdu	2229	4	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2229	5	is	be	VBZ
cord-014597-66vd2mdu	2229	6	an	an	DT
cord-014597-66vd2mdu	2229	7	efficient	efficient	JJ
cord-014597-66vd2mdu	2229	8	bench	bench	NN
cord-014597-66vd2mdu	2229	9	-	-	HYPH
cord-014597-66vd2mdu	2229	10	top	top	NN
cord-014597-66vd2mdu	2229	11	system	system	NN
cord-014597-66vd2mdu	2229	12	the	the	DT
cord-014597-66vd2mdu	2229	13	results	result	NNS
cord-014597-66vd2mdu	2229	14	of	of	IN
cord-014597-66vd2mdu	2229	15	which	which	WDT
cord-014597-66vd2mdu	2229	16	can	can	MD
cord-014597-66vd2mdu	2229	17	be	be	VB
cord-014597-66vd2mdu	2229	18	readily	readily	RB
cord-014597-66vd2mdu	2229	19	scaled	scale	VBN
cord-014597-66vd2mdu	2229	20	to	to	IN
cord-014597-66vd2mdu	2229	21	the	the	DT
cord-014597-66vd2mdu	2229	22	iCELLis	iCELLis	NNP
cord-014597-66vd2mdu	2229	23	500	500	CD
cord-014597-66vd2mdu	2229	24	system	system	NN
cord-014597-66vd2mdu	2229	25	.	.	.
cord-014597-66vd2mdu	2230	1	Background	Background	NNP
cord-014597-66vd2mdu	2230	2	TissUse	TissUse	NNP
cord-014597-66vd2mdu	2230	3	Multi	Multi	NNP
cord-014597-66vd2mdu	2230	4	-	-	HYPH
cord-014597-66vd2mdu	2230	5	Organ	Organ	NNP
cord-014597-66vd2mdu	2230	6	-	-	HYPH
cord-014597-66vd2mdu	2230	7	Chip	Chip	NNP
cord-014597-66vd2mdu	2230	8	(	(	-LRB-
cord-014597-66vd2mdu	2230	9	MOC	MOC	NNP
cord-014597-66vd2mdu	2230	10	)	)	-RRB-
cord-014597-66vd2mdu	2230	11	platform	platform	NN
cord-014597-66vd2mdu	2230	12	contributes	contribute	VBZ
cord-014597-66vd2mdu	2230	13	to	to	IN
cord-014597-66vd2mdu	2230	14	the	the	DT
cord-014597-66vd2mdu	2230	15	ongoing	ongoing	JJ
cord-014597-66vd2mdu	2230	16	advancement	advancement	NN
cord-014597-66vd2mdu	2230	17	in	in	IN
cord-014597-66vd2mdu	2230	18	systemic	systemic	JJ
cord-014597-66vd2mdu	2230	19	substance	substance	NN
cord-014597-66vd2mdu	2230	20	testing	testing	NN
cord-014597-66vd2mdu	2230	21	in	in	IN
cord-014597-66vd2mdu	2230	22	vitro	vitro	FW
cord-014597-66vd2mdu	2230	23	.	.	.
cord-014597-66vd2mdu	2231	1	Current	current	JJ
cord-014597-66vd2mdu	2231	2	in	in	IN
cord-014597-66vd2mdu	2231	3	vitro	vitro	FW
cord-014597-66vd2mdu	2231	4	and	and	CC
cord-014597-66vd2mdu	2231	5	animal	animal	NN
cord-014597-66vd2mdu	2231	6	tests	test	NNS
cord-014597-66vd2mdu	2231	7	for	for	IN
cord-014597-66vd2mdu	2231	8	drug	drug	NN
cord-014597-66vd2mdu	2231	9	development	development	NN
cord-014597-66vd2mdu	2231	10	are	be	VBP
cord-014597-66vd2mdu	2231	11	failing	fail	VBG
cord-014597-66vd2mdu	2231	12	to	to	TO
cord-014597-66vd2mdu	2231	13	emulate	emulate	VB
cord-014597-66vd2mdu	2231	14	the	the	DT
cord-014597-66vd2mdu	2231	15	systemic	systemic	JJ
cord-014597-66vd2mdu	2231	16	organ	organ	NN
cord-014597-66vd2mdu	2231	17	complexity	complexity	NN
cord-014597-66vd2mdu	2231	18	of	of	IN
cord-014597-66vd2mdu	2231	19	the	the	DT
cord-014597-66vd2mdu	2231	20	human	human	JJ
cord-014597-66vd2mdu	2231	21	body	body	NN
cord-014597-66vd2mdu	2231	22	and	and	CC
cord-014597-66vd2mdu	2231	23	,	,	,
cord-014597-66vd2mdu	2231	24	therefore	therefore	RB
cord-014597-66vd2mdu	2231	25	,	,	,
cord-014597-66vd2mdu	2231	26	often	often	RB
cord-014597-66vd2mdu	2231	27	do	do	VBP
cord-014597-66vd2mdu	2231	28	not	not	RB
cord-014597-66vd2mdu	2231	29	accurately	accurately	RB
cord-014597-66vd2mdu	2231	30	predict	predict	VB
cord-014597-66vd2mdu	2231	31	drug	drug	NN
cord-014597-66vd2mdu	2231	32	toxicity	toxicity	NN
cord-014597-66vd2mdu	2231	33	.	.	.
cord-014597-66vd2mdu	2232	1	Especially	especially	RB
cord-014597-66vd2mdu	2232	2	,	,	,
cord-014597-66vd2mdu	2232	3	cardiotoxicity	cardiotoxicity	NN
cord-014597-66vd2mdu	2232	4	is	be	VBZ
cord-014597-66vd2mdu	2232	5	one	one	CD
cord-014597-66vd2mdu	2232	6	of	of	IN
cord-014597-66vd2mdu	2232	7	the	the	DT
cord-014597-66vd2mdu	2232	8	main	main	JJ
cord-014597-66vd2mdu	2232	9	reasons	reason	NNS
cord-014597-66vd2mdu	2232	10	why	why	WRB
cord-014597-66vd2mdu	2232	11	new	new	JJ
cord-014597-66vd2mdu	2232	12	compounds	compound	NNS
cord-014597-66vd2mdu	2232	13	are	be	VBP
cord-014597-66vd2mdu	2232	14	failing	fail	VBG
cord-014597-66vd2mdu	2232	15	in	in	IN
cord-014597-66vd2mdu	2232	16	clinical	clinical	JJ
cord-014597-66vd2mdu	2232	17	trials	trial	NNS
cord-014597-66vd2mdu	2232	18	.	.	.
cord-014597-66vd2mdu	2233	1	Therefore	therefore	RB
cord-014597-66vd2mdu	2233	2	,	,	,
cord-014597-66vd2mdu	2233	3	we	-PRON-	PRP
cord-014597-66vd2mdu	2233	4	aimed	aim	VBD
cord-014597-66vd2mdu	2233	5	to	to	TO
cord-014597-66vd2mdu	2233	6	establish	establish	VB
cord-014597-66vd2mdu	2233	7	an	an	DT
cord-014597-66vd2mdu	2233	8	autologous	autologous	JJ
cord-014597-66vd2mdu	2233	9	dynamic	dynamic	JJ
cord-014597-66vd2mdu	2233	10	multiorgan	multiorgan	NN
cord-014597-66vd2mdu	2233	11	-	-	HYPH
cord-014597-66vd2mdu	2233	12	device	device	NN
cord-014597-66vd2mdu	2233	13	integrating	integrate	VBG
cord-014597-66vd2mdu	2233	14	cardiomyocytes	cardiomyocyte	NNS
cord-014597-66vd2mdu	2233	15	for	for	IN
cord-014597-66vd2mdu	2233	16	substance	substance	NN
cord-014597-66vd2mdu	2233	17	testing	testing	NN
cord-014597-66vd2mdu	2233	18	.	.	.
cord-014597-66vd2mdu	2234	1	Generic	generic	JJ
cord-014597-66vd2mdu	2234	2	2D	2d	NN
cord-014597-66vd2mdu	2234	3	monolayer	monolayer	NN
cord-014597-66vd2mdu	2234	4	and	and	CC
cord-014597-66vd2mdu	2234	5	3D	3D	NNP
cord-014597-66vd2mdu	2234	6	suspension	suspension	NN
cord-014597-66vd2mdu	2234	7	iPSC	iPSC	NNP
cord-014597-66vd2mdu	2234	8	derived	derive	VBN
cord-014597-66vd2mdu	2234	9	cardiomyocytes	cardiomyocyte	NNS
cord-014597-66vd2mdu	2234	10	differentiation	differentiation	NN
cord-014597-66vd2mdu	2234	11	protocols	protocol	NNS
cord-014597-66vd2mdu	2234	12	were	be	VBD
cord-014597-66vd2mdu	2234	13	established	establish	VBN
cord-014597-66vd2mdu	2234	14	.	.	.
cord-014597-66vd2mdu	2235	1	Beating	beat	VBG
cord-014597-66vd2mdu	2235	2	cardiomyocytes	cardiomyocyte	NNS
cord-014597-66vd2mdu	2235	3	were	be	VBD
cord-014597-66vd2mdu	2235	4	first	first	RB
cord-014597-66vd2mdu	2235	5	seen	see	VBN
cord-014597-66vd2mdu	2235	6	on	on	IN
cord-014597-66vd2mdu	2235	7	day	day	NN
cord-014597-66vd2mdu	2235	8	8	8	CD
cord-014597-66vd2mdu	2235	9	in	in	IN
cord-014597-66vd2mdu	2235	10	monolayer	monolayer	NN
cord-014597-66vd2mdu	2235	11	as	as	RB
cord-014597-66vd2mdu	2235	12	well	well	RB
cord-014597-66vd2mdu	2235	13	as	as	IN
cord-014597-66vd2mdu	2235	14	in	in	IN
cord-014597-66vd2mdu	2235	15	spheroid	spheroid	NN
cord-014597-66vd2mdu	2235	16	culture	culture	NN
cord-014597-66vd2mdu	2235	17	.	.	.
cord-014597-66vd2mdu	2236	1	Cardiomyocytes	cardiomyocyte	NNS
cord-014597-66vd2mdu	2236	2	show	show	VBP
cord-014597-66vd2mdu	2236	3	up	up	RP
cord-014597-66vd2mdu	2236	4	to	to	TO
cord-014597-66vd2mdu	2236	5	64	64	CD
cord-014597-66vd2mdu	2236	6	%	%	NN
cord-014597-66vd2mdu	2236	7	cardiac	cardiac	NN
cord-014597-66vd2mdu	2236	8	troponin	troponin	VBD
cord-014597-66vd2mdu	2236	9	T	T	NNP
cord-014597-66vd2mdu	2236	10	positive	positive	JJ
cord-014597-66vd2mdu	2236	11	cells	cell	NNS
cord-014597-66vd2mdu	2236	12	and	and	CC
cord-014597-66vd2mdu	2236	13	44	44	CD
cord-014597-66vd2mdu	2236	14	%	%	NN
cord-014597-66vd2mdu	2236	15	myosin	myosin	NN
cord-014597-66vd2mdu	2236	16	heavy	heavy	JJ
cord-014597-66vd2mdu	2236	17	chain	chain	NN
cord-014597-66vd2mdu	2236	18	positive	positive	JJ
cord-014597-66vd2mdu	2236	19	cells	cell	NNS
cord-014597-66vd2mdu	2236	20	by	by	IN
cord-014597-66vd2mdu	2236	21	flow	flow	NN
cord-014597-66vd2mdu	2236	22	cytometry	cytometry	NN
cord-014597-66vd2mdu	2236	23	(	(	-LRB-
cord-014597-66vd2mdu	2236	24	Fig	fig	NN
cord-014597-66vd2mdu	2236	25	.	.	.
cord-014597-66vd2mdu	2236	26	1	1	CD
cord-014597-66vd2mdu	2236	27	g	g	NN
cord-014597-66vd2mdu	2236	28	,	,	,
cord-014597-66vd2mdu	2236	29	h	h	NNP
cord-014597-66vd2mdu	2236	30	)	)	-RRB-
cord-014597-66vd2mdu	2236	31	.	.	.
cord-014597-66vd2mdu	2237	1	Myosin	myosin	NN
cord-014597-66vd2mdu	2237	2	II	II	NNP
cord-014597-66vd2mdu	2237	3	heavy	heavy	JJ
cord-014597-66vd2mdu	2237	4	chain	chain	NN
cord-014597-66vd2mdu	2237	5	,	,	,
cord-014597-66vd2mdu	2237	6	α	α	NN
cord-014597-66vd2mdu	2237	7	-	-	HYPH
cord-014597-66vd2mdu	2237	8	actinin	actinin	NNP
cord-014597-66vd2mdu	2237	9	,	,	,
cord-014597-66vd2mdu	2237	10	myosin	myosin	NN
cord-014597-66vd2mdu	2237	11	9/10	9/10	CD
cord-014597-66vd2mdu	2237	12	,	,	,
cord-014597-66vd2mdu	2237	13	myosin	myosin	NN
cord-014597-66vd2mdu	2237	14	11	11	CD
cord-014597-66vd2mdu	2237	15	and	and	CC
cord-014597-66vd2mdu	2237	16	caldesmon	caldesmon	JJ
cord-014597-66vd2mdu	2237	17	expression	expression	NN
cord-014597-66vd2mdu	2237	18	was	be	VBD
cord-014597-66vd2mdu	2237	19	shown	show	VBN
cord-014597-66vd2mdu	2237	20	by	by	IN
cord-014597-66vd2mdu	2237	21	immunohistochemistry	immunohistochemistry	FW
cord-014597-66vd2mdu	2237	22	(	(	-LRB-
cord-014597-66vd2mdu	2237	23	Fig	Fig	NNP
cord-014597-66vd2mdu	2237	24	.	.	.
cord-014597-66vd2mdu	2237	25	1a	1a	CD
cord-014597-66vd2mdu	2237	26	-	-	HYPH
cord-014597-66vd2mdu	2237	27	d	d	NN
cord-014597-66vd2mdu	2237	28	)	)	-RRB-
cord-014597-66vd2mdu	2237	29	.	.	.
cord-014597-66vd2mdu	2238	1	Due	due	IN
cord-014597-66vd2mdu	2238	2	to	to	IN
cord-014597-66vd2mdu	2238	3	the	the	DT
cord-014597-66vd2mdu	2238	4	exclusion	exclusion	NN
cord-014597-66vd2mdu	2238	5	of	of	IN
cord-014597-66vd2mdu	2238	6	a	a	DT
cord-014597-66vd2mdu	2238	7	lactate	lactate	NN
cord-014597-66vd2mdu	2238	8	enrichment	enrichment	NN
cord-014597-66vd2mdu	2238	9	of	of	IN
cord-014597-66vd2mdu	2238	10	cardiomyocytes	cardiomyocyte	NNS
cord-014597-66vd2mdu	2238	11	,	,	,
cord-014597-66vd2mdu	2238	12	cardiac	cardiac	JJ
cord-014597-66vd2mdu	2238	13	fibroblasts	fibroblast	NNS
cord-014597-66vd2mdu	2238	14	are	be	VBP
cord-014597-66vd2mdu	2238	15	also	also	RB
cord-014597-66vd2mdu	2238	16	expressed	express	VBN
cord-014597-66vd2mdu	2238	17	in	in	IN
cord-014597-66vd2mdu	2238	18	the	the	DT
cord-014597-66vd2mdu	2238	19	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2238	20	shown	show	VBN
cord-014597-66vd2mdu	2238	21	by	by	IN
cord-014597-66vd2mdu	2238	22	vimentin	vimentin	NN
cord-014597-66vd2mdu	2238	23	staining	staining	NN
cord-014597-66vd2mdu	2238	24	.	.	.
cord-014597-66vd2mdu	2239	1	Those	those	DT
cord-014597-66vd2mdu	2239	2	cardiac	cardiac	JJ
cord-014597-66vd2mdu	2239	3	fibroblasts	fibroblast	NNS
cord-014597-66vd2mdu	2239	4	lead	lead	VBP
cord-014597-66vd2mdu	2239	5	to	to	IN
cord-014597-66vd2mdu	2239	6	a	a	DT
cord-014597-66vd2mdu	2239	7	physiological	physiological	JJ
cord-014597-66vd2mdu	2239	8	heterologous	heterologous	JJ
cord-014597-66vd2mdu	2239	9	cell	cell	NN
cord-014597-66vd2mdu	2239	10	population	population	NN
cord-014597-66vd2mdu	2239	11	similar	similar	JJ
cord-014597-66vd2mdu	2239	12	to	to	IN
cord-014597-66vd2mdu	2239	13	the	the	DT
cord-014597-66vd2mdu	2239	14	human	human	JJ
cord-014597-66vd2mdu	2239	15	heart	heart	NN
cord-014597-66vd2mdu	2239	16	.	.	.
cord-014597-66vd2mdu	2240	1	Beating	beat	VBG
cord-014597-66vd2mdu	2240	2	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2240	3	were	be	VBD
cord-014597-66vd2mdu	2240	4	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2240	5	for	for	IN
cord-014597-66vd2mdu	2240	6	7	7	CD
cord-014597-66vd2mdu	2240	7	days	day	NNS
cord-014597-66vd2mdu	2240	8	under	under	IN
cord-014597-66vd2mdu	2240	9	dynamic	dynamic	JJ
cord-014597-66vd2mdu	2240	10	culture	culture	NN
cord-014597-66vd2mdu	2240	11	conditions	condition	NNS
cord-014597-66vd2mdu	2240	12	in	in	IN
cord-014597-66vd2mdu	2240	13	the	the	DT
cord-014597-66vd2mdu	2240	14	Multi	Multi	NNP
cord-014597-66vd2mdu	2240	15	-	-	NNP
cord-014597-66vd2mdu	2240	16	Organ	Organ	NNP
cord-014597-66vd2mdu	2240	17	-	-	HYPH
cord-014597-66vd2mdu	2240	18	Chip	Chip	NNP
cord-014597-66vd2mdu	2240	19	.	.	.
cord-014597-66vd2mdu	2241	1	The	the	DT
cord-014597-66vd2mdu	2241	2	integrated	integrate	VBN
cord-014597-66vd2mdu	2241	3	on	on	IN
cord-014597-66vd2mdu	2241	4	-	-	HYPH
cord-014597-66vd2mdu	2241	5	chip	chip	NN
cord-014597-66vd2mdu	2241	6	micropump	micropump	NN
cord-014597-66vd2mdu	2241	7	provides	provide	VBZ
cord-014597-66vd2mdu	2241	8	physiological	physiological	JJ
cord-014597-66vd2mdu	2241	9	-	-	HYPH
cord-014597-66vd2mdu	2241	10	like	like	JJ
cord-014597-66vd2mdu	2241	11	pulsatile	pulsatile	JJ
cord-014597-66vd2mdu	2241	12	circulation	circulation	NN
cord-014597-66vd2mdu	2241	13	at	at	IN
cord-014597-66vd2mdu	2241	14	a	a	DT
cord-014597-66vd2mdu	2241	15	microliter	microliter	JJ
cord-014597-66vd2mdu	2241	16	scale	scale	NN
cord-014597-66vd2mdu	2241	17	and	and	CC
cord-014597-66vd2mdu	2241	18	leads	lead	VBZ
cord-014597-66vd2mdu	2241	19	to	to	IN
cord-014597-66vd2mdu	2241	20	better	well	JJR
cord-014597-66vd2mdu	2241	21	nutrition	nutrition	NN
cord-014597-66vd2mdu	2241	22	and	and	CC
cord-014597-66vd2mdu	2241	23	oxygen	oxygen	NN
cord-014597-66vd2mdu	2241	24	supply	supply	NN
cord-014597-66vd2mdu	2241	25	.	.	.
cord-014597-66vd2mdu	2242	1	The	the	DT
cord-014597-66vd2mdu	2242	2	next	next	JJ
cord-014597-66vd2mdu	2242	3	significant	significant	JJ
cord-014597-66vd2mdu	2242	4	step	step	NN
cord-014597-66vd2mdu	2242	5	is	be	VBZ
cord-014597-66vd2mdu	2242	6	to	to	TO
cord-014597-66vd2mdu	2242	7	combine	combine	VB
cord-014597-66vd2mdu	2242	8	multiple	multiple	JJ
cord-014597-66vd2mdu	2242	9	autologous	autologous	JJ
cord-014597-66vd2mdu	2242	10	3D	3d	JJ
cord-014597-66vd2mdu	2242	11	organ	organ	NN
cord-014597-66vd2mdu	2242	12	equivalents	equivalent	NNS
cord-014597-66vd2mdu	2242	13	in	in	IN
cord-014597-66vd2mdu	2242	14	our	-PRON-	PRP$
cord-014597-66vd2mdu	2242	15	Multi	multi	JJ
cord-014597-66vd2mdu	2242	16	-	-	HYPH
cord-014597-66vd2mdu	2242	17	Organ	Organ	NNP
cord-014597-66vd2mdu	2242	18	-	-	HYPH
cord-014597-66vd2mdu	2242	19	Chip	Chip	NNP
cord-014597-66vd2mdu	2242	20	using	use	VBG
cord-014597-66vd2mdu	2242	21	iPSC	ipsc	JJ
cord-014597-66vd2mdu	2242	22	differentiation	differentiation	NN
cord-014597-66vd2mdu	2242	23	technology	technology	NN
cord-014597-66vd2mdu	2242	24	.	.	.
cord-014597-66vd2mdu	2243	1	Differentiating	differentiate	VBG
cord-014597-66vd2mdu	2243	2	all	all	DT
cord-014597-66vd2mdu	2243	3	cell	cell	NN
cord-014597-66vd2mdu	2243	4	types	type	NNS
cord-014597-66vd2mdu	2243	5	from	from	IN
cord-014597-66vd2mdu	2243	6	one	one	CD
cord-014597-66vd2mdu	2243	7	iPSC	ipsc	JJ
cord-014597-66vd2mdu	2243	8	donor	donor	NN
cord-014597-66vd2mdu	2243	9	is	be	VBZ
cord-014597-66vd2mdu	2243	10	crucial	crucial	JJ
cord-014597-66vd2mdu	2243	11	to	to	TO
cord-014597-66vd2mdu	2243	12	overcome	overcome	VB
cord-014597-66vd2mdu	2243	13	source	source	NN
cord-014597-66vd2mdu	2243	14	and	and	CC
cord-014597-66vd2mdu	2243	15	rejection	rejection	NN
cord-014597-66vd2mdu	2243	16	problems	problem	NNS
cord-014597-66vd2mdu	2243	17	.	.	.
cord-014597-66vd2mdu	2244	1	Combining	combine	VBG
cord-014597-66vd2mdu	2244	2	our	-PRON-	PRP$
cord-014597-66vd2mdu	2244	3	Multi	multi	JJ
cord-014597-66vd2mdu	2244	4	-	-	HYPH
cord-014597-66vd2mdu	2244	5	Organ	Organ	NNP
cord-014597-66vd2mdu	2244	6	-	-	HYPH
cord-014597-66vd2mdu	2244	7	Chip	Chip	NNP
cord-014597-66vd2mdu	2244	8	platform	platform	NN
cord-014597-66vd2mdu	2244	9	with	with	IN
cord-014597-66vd2mdu	2244	10	iPSC	ipsc	JJ
cord-014597-66vd2mdu	2244	11	differentiation	differentiation	NN
cord-014597-66vd2mdu	2244	12	technology	technology	NN
cord-014597-66vd2mdu	2244	13	will	will	MD
cord-014597-66vd2mdu	2244	14	eventually	eventually	RB
cord-014597-66vd2mdu	2244	15	lead	lead	VB
cord-014597-66vd2mdu	2244	16	to	to	IN
cord-014597-66vd2mdu	2244	17	a	a	DT
cord-014597-66vd2mdu	2244	18	personalized	personalized	JJ
cord-014597-66vd2mdu	2244	19	system	system	NN
cord-014597-66vd2mdu	2244	20	for	for	IN
cord-014597-66vd2mdu	2244	21	drug	drug	NN
cord-014597-66vd2mdu	2244	22	and	and	CC
cord-014597-66vd2mdu	2244	23	substance	substance	NN
cord-014597-66vd2mdu	2244	24	testing	testing	NN
cord-014597-66vd2mdu	2244	25	.	.	.
cord-014597-66vd2mdu	2245	1	Lab	lab	NN
cord-014597-66vd2mdu	2245	2	as	as	IN
cord-014597-66vd2mdu	2245	3	a	a	DT
cord-014597-66vd2mdu	2245	4	service	service	NN
cord-014597-66vd2mdu	2245	5	-automated	-automated	CD
cord-014597-66vd2mdu	2245	6	cell	cell	NN
cord-014597-66vd2mdu	2245	7	-	-	HYPH
cord-014597-66vd2mdu	2245	8	based	base	VBN
cord-014597-66vd2mdu	2245	9	assays	assay	NNS
cord-014597-66vd2mdu	2245	10	Lena	Lena	NNP
cord-014597-66vd2mdu	2245	11	Schober	Schober	NNP
cord-014597-66vd2mdu	2245	12	,	,	,
cord-014597-66vd2mdu	2245	13	Moriz	Moriz	NNP
cord-014597-66vd2mdu	2245	14	Walter	Walter	NNP
cord-014597-66vd2mdu	2245	15	,	,	,
cord-014597-66vd2mdu	2245	16	Andrea	Andrea	NNP
cord-014597-66vd2mdu	2245	17	Traube	Traube	NNP
cord-014597-66vd2mdu	2245	18	Laboratory	Laboratory	NNP
cord-014597-66vd2mdu	2245	19	Automation	Automation	NNP
cord-014597-66vd2mdu	2245	20	and	and	CC
cord-014597-66vd2mdu	2245	21	Biomanufacturing	Biomanufacturing	NNP
cord-014597-66vd2mdu	2245	22	Engineering	Engineering	NNP
cord-014597-66vd2mdu	2245	23	,	,	,
cord-014597-66vd2mdu	2245	24	Fraunhofer	Fraunhofer	NNP
cord-014597-66vd2mdu	2245	25	IPA	IPA	NNP
cord-014597-66vd2mdu	2245	26	,	,	,
cord-014597-66vd2mdu	2246	1	Stuttgart	Stuttgart	NNP
cord-014597-66vd2mdu	2246	2	,	,	,
cord-014597-66vd2mdu	2246	3	Germany	Germany	NNP
cord-014597-66vd2mdu	2246	4	Correspondence	Correspondence	NNP
cord-014597-66vd2mdu	2246	5	:	:	:
cord-014597-66vd2mdu	2247	1	Lena	Lena	NNP
cord-014597-66vd2mdu	2247	2	Schober	Schober	NNP
cord-014597-66vd2mdu	2247	3	(	(	-LRB-
cord-014597-66vd2mdu	2247	4	lena.schober@ipa.fraunhofer.de	lena.schober@ipa.fraunhofer.de	NNP
cord-014597-66vd2mdu	2247	5	)	)	-RRB-
cord-014597-66vd2mdu	2248	1	BMC	BMC	NNP
cord-014597-66vd2mdu	2248	2	Proceedings	Proceedings	NNP
cord-014597-66vd2mdu	2248	3	2018	2018	CD
cord-014597-66vd2mdu	2248	4	,	,	,
cord-014597-66vd2mdu	2249	1	12(Suppl	12(suppl	CD
cord-014597-66vd2mdu	2249	2	1):P-365	1):p-365	CD
cord-014597-66vd2mdu	2250	1	Background	background	NN
cord-014597-66vd2mdu	2251	1	The	the	DT
cord-014597-66vd2mdu	2251	2	use	use	NN
cord-014597-66vd2mdu	2251	3	of	of	IN
cord-014597-66vd2mdu	2251	4	cell	cell	NN
cord-014597-66vd2mdu	2251	5	-	-	HYPH
cord-014597-66vd2mdu	2251	6	based	base	VBN
cord-014597-66vd2mdu	2251	7	assays	assay	NNS
cord-014597-66vd2mdu	2251	8	in	in	IN
cord-014597-66vd2mdu	2251	9	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	2251	10	industry	industry	NN
cord-014597-66vd2mdu	2251	11	and	and	CC
cord-014597-66vd2mdu	2251	12	academic	academic	JJ
cord-014597-66vd2mdu	2251	13	research	research	NN
cord-014597-66vd2mdu	2251	14	is	be	VBZ
cord-014597-66vd2mdu	2251	15	a	a	DT
cord-014597-66vd2mdu	2251	16	growing	grow	VBG
cord-014597-66vd2mdu	2251	17	trend	trend	NN
cord-014597-66vd2mdu	2251	18	that	that	WDT
cord-014597-66vd2mdu	2251	19	is	be	VBZ
cord-014597-66vd2mdu	2251	20	a	a	DT
cord-014597-66vd2mdu	2251	21	driving	drive	VBG
cord-014597-66vd2mdu	2251	22	force	force	NN
cord-014597-66vd2mdu	2251	23	to	to	TO
cord-014597-66vd2mdu	2251	24	reduce	reduce	VB
cord-014597-66vd2mdu	2251	25	costs	cost	NNS
cord-014597-66vd2mdu	2251	26	for	for	IN
cord-014597-66vd2mdu	2251	27	drug	drug	NN
cord-014597-66vd2mdu	2251	28	development	development	NN
cord-014597-66vd2mdu	2251	29	.	.	.
cord-014597-66vd2mdu	2252	1	Academic	academic	JJ
cord-014597-66vd2mdu	2252	2	research	research	NN
cord-014597-66vd2mdu	2252	3	is	be	VBZ
cord-014597-66vd2mdu	2252	4	gaining	gain	VBG
cord-014597-66vd2mdu	2252	5	information	information	NN
cord-014597-66vd2mdu	2252	6	about	about	IN
cord-014597-66vd2mdu	2252	7	intracellular	intracellular	JJ
cord-014597-66vd2mdu	2252	8	targets	target	NNS
cord-014597-66vd2mdu	2252	9	or	or	CC
cord-014597-66vd2mdu	2252	10	functional	functional	JJ
cord-014597-66vd2mdu	2252	11	mechanisms	mechanism	NNS
cord-014597-66vd2mdu	2252	12	through	through	IN
cord-014597-66vd2mdu	2252	13	the	the	DT
cord-014597-66vd2mdu	2252	14	variety	variety	NN
cord-014597-66vd2mdu	2252	15	of	of	IN
cord-014597-66vd2mdu	2252	16	different	different	JJ
cord-014597-66vd2mdu	2252	17	assays	assay	NNS
cord-014597-66vd2mdu	2252	18	.	.	.
cord-014597-66vd2mdu	2253	1	These	these	DT
cord-014597-66vd2mdu	2253	2	benefits	benefit	NNS
cord-014597-66vd2mdu	2253	3	can	can	MD
cord-014597-66vd2mdu	2253	4	be	be	VB
cord-014597-66vd2mdu	2253	5	used	use	VBN
cord-014597-66vd2mdu	2253	6	in	in	IN
cord-014597-66vd2mdu	2253	7	preclinical	preclinical	JJ
cord-014597-66vd2mdu	2253	8	studies	study	NNS
cord-014597-66vd2mdu	2253	9	and	and	CC
cord-014597-66vd2mdu	2253	10	furthermore	furthermore	RB
cord-014597-66vd2mdu	2253	11	costly	costly	JJ
cord-014597-66vd2mdu	2253	12	late	late	JJ
cord-014597-66vd2mdu	2253	13	-	-	HYPH
cord-014597-66vd2mdu	2253	14	stage	stage	NN
cord-014597-66vd2mdu	2253	15	drug	drug	NN
cord-014597-66vd2mdu	2253	16	failures	failure	NNS
cord-014597-66vd2mdu	2253	17	may	may	MD
cord-014597-66vd2mdu	2253	18	be	be	VB
cord-014597-66vd2mdu	2253	19	reduced	reduce	VBN
cord-014597-66vd2mdu	2253	20	by	by	IN
cord-014597-66vd2mdu	2253	21	the	the	DT
cord-014597-66vd2mdu	2253	22	use	use	NN
cord-014597-66vd2mdu	2253	23	of	of	IN
cord-014597-66vd2mdu	2253	24	cell	cell	NN
cord-014597-66vd2mdu	2253	25	-	-	HYPH
cord-014597-66vd2mdu	2253	26	based	base	VBN
cord-014597-66vd2mdu	2253	27	assays	assay	NNS
cord-014597-66vd2mdu	2253	28	.	.	.
cord-014597-66vd2mdu	2254	1	The	the	DT
cord-014597-66vd2mdu	2254	2	use	use	NN
cord-014597-66vd2mdu	2254	3	of	of	IN
cord-014597-66vd2mdu	2254	4	automated	automate	VBN
cord-014597-66vd2mdu	2254	5	systems	system	NNS
cord-014597-66vd2mdu	2254	6	is	be	VBZ
cord-014597-66vd2mdu	2254	7	also	also	RB
cord-014597-66vd2mdu	2254	8	in	in	IN
cord-014597-66vd2mdu	2254	9	great	great	JJ
cord-014597-66vd2mdu	2254	10	demand	demand	NN
cord-014597-66vd2mdu	2254	11	and	and	CC
cord-014597-66vd2mdu	2254	12	will	will	MD
cord-014597-66vd2mdu	2254	13	change	change	VB
cord-014597-66vd2mdu	2254	14	the	the	DT
cord-014597-66vd2mdu	2254	15	testing	testing	NN
cord-014597-66vd2mdu	2254	16	of	of	IN
cord-014597-66vd2mdu	2254	17	substances	substance	NNS
cord-014597-66vd2mdu	2254	18	and	and	CC
cord-014597-66vd2mdu	2254	19	research	research	NN
cord-014597-66vd2mdu	2254	20	activities	activity	NNS
cord-014597-66vd2mdu	2254	21	.	.	.
cord-014597-66vd2mdu	2255	1	Nevertheless	nevertheless	RB
cord-014597-66vd2mdu	2255	2	,	,	,
cord-014597-66vd2mdu	2255	3	there	there	EX
cord-014597-66vd2mdu	2255	4	are	be	VBP
cord-014597-66vd2mdu	2255	5	a	a	DT
cord-014597-66vd2mdu	2255	6	lot	lot	NN
cord-014597-66vd2mdu	2255	7	of	of	IN
cord-014597-66vd2mdu	2255	8	barriers	barrier	NNS
cord-014597-66vd2mdu	2255	9	at	at	IN
cord-014597-66vd2mdu	2255	10	the	the	DT
cord-014597-66vd2mdu	2255	11	moment	moment	NN
cord-014597-66vd2mdu	2255	12	limiting	limit	VBG
cord-014597-66vd2mdu	2255	13	the	the	DT
cord-014597-66vd2mdu	2255	14	successful	successful	JJ
cord-014597-66vd2mdu	2255	15	application	application	NN
cord-014597-66vd2mdu	2255	16	of	of	IN
cord-014597-66vd2mdu	2255	17	automated	automate	VBN
cord-014597-66vd2mdu	2255	18	systems	system	NNS
cord-014597-66vd2mdu	2255	19	in	in	IN
cord-014597-66vd2mdu	2255	20	this	this	DT
cord-014597-66vd2mdu	2255	21	field	field	NN
cord-014597-66vd2mdu	2255	22	.	.	.
cord-014597-66vd2mdu	2256	1	By	by	IN
cord-014597-66vd2mdu	2256	2	the	the	DT
cord-014597-66vd2mdu	2256	3	lack	lack	NN
cord-014597-66vd2mdu	2256	4	of	of	IN
cord-014597-66vd2mdu	2256	5	flexibility	flexibility	NN
cord-014597-66vd2mdu	2256	6	and	and	CC
cord-014597-66vd2mdu	2256	7	the	the	DT
cord-014597-66vd2mdu	2256	8	demand	demand	NN
cord-014597-66vd2mdu	2256	9	for	for	IN
cord-014597-66vd2mdu	2256	10	skilled	skilled	JJ
cord-014597-66vd2mdu	2256	11	computer	computer	NN
cord-014597-66vd2mdu	2256	12	scientists	scientist	NNS
cord-014597-66vd2mdu	2256	13	&	&	CC
cord-014597-66vd2mdu	2256	14	engineers	engineer	NNS
cord-014597-66vd2mdu	2256	15	just	just	RB
cord-014597-66vd2mdu	2256	16	the	the	DT
cord-014597-66vd2mdu	2256	17	two	two	CD
cord-014597-66vd2mdu	2256	18	main	main	JJ
cord-014597-66vd2mdu	2256	19	aspects	aspect	NNS
cord-014597-66vd2mdu	2256	20	stated	state	VBN
cord-014597-66vd2mdu	2256	21	by	by	IN
cord-014597-66vd2mdu	2256	22	experts	expert	NNS
cord-014597-66vd2mdu	2256	23	shall	shall	MD
cord-014597-66vd2mdu	2256	24	be	be	VB
cord-014597-66vd2mdu	2256	25	mentioned	mention	VBN
cord-014597-66vd2mdu	2256	26	.	.	.
cord-014597-66vd2mdu	2257	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	2257	2	strong	strong	JJ
cord-014597-66vd2mdu	2257	3	background	background	NN
cord-014597-66vd2mdu	2257	4	on	on	IN
cord-014597-66vd2mdu	2257	5	automated	automated	JJ
cord-014597-66vd2mdu	2257	6	cell	cell	NN
cord-014597-66vd2mdu	2257	7	culture	culture	NN
cord-014597-66vd2mdu	2257	8	technologies	technology	NNS
cord-014597-66vd2mdu	2257	9	and	and	CC
cord-014597-66vd2mdu	2257	10	expertise	expertise	NN
cord-014597-66vd2mdu	2257	11	,	,	,
cord-014597-66vd2mdu	2257	12	gained	gain	VBN
cord-014597-66vd2mdu	2257	13	in	in	IN
cord-014597-66vd2mdu	2257	14	several	several	JJ
cord-014597-66vd2mdu	2257	15	projects	project	NNS
cord-014597-66vd2mdu	2257	16	,	,	,
cord-014597-66vd2mdu	2257	17	let	let	VB
cord-014597-66vd2mdu	2257	18	us	-PRON-	PRP
cord-014597-66vd2mdu	2257	19	rethink	rethink	VB
cord-014597-66vd2mdu	2257	20	the	the	DT
cord-014597-66vd2mdu	2257	21	overall	overall	JJ
cord-014597-66vd2mdu	2257	22	process	process	NN
cord-014597-66vd2mdu	2257	23	chain	chain	NN
cord-014597-66vd2mdu	2257	24	and	and	CC
cord-014597-66vd2mdu	2257	25	overcome	overcome	VB
cord-014597-66vd2mdu	2257	26	established	establish	VBD
cord-014597-66vd2mdu	2257	27	principles	principle	NNS
cord-014597-66vd2mdu	2257	28	.	.	.
cord-014597-66vd2mdu	2258	1	A	a	DT
cord-014597-66vd2mdu	2258	2	new	new	JJ
cord-014597-66vd2mdu	2258	3	service	service	NN
cord-014597-66vd2mdu	2258	4	orientated	orientate	VBN
cord-014597-66vd2mdu	2258	5	platform	platform	NN
cord-014597-66vd2mdu	2258	6	for	for	IN
cord-014597-66vd2mdu	2258	7	the	the	DT
cord-014597-66vd2mdu	2258	8	execution	execution	NN
cord-014597-66vd2mdu	2258	9	of	of	IN
cord-014597-66vd2mdu	2258	10	cell	cell	NN
cord-014597-66vd2mdu	2258	11	-	-	HYPH
cord-014597-66vd2mdu	2258	12	based	base	VBN
cord-014597-66vd2mdu	2258	13	assays	assay	NNS
cord-014597-66vd2mdu	2258	14	that	that	WDT
cord-014597-66vd2mdu	2258	15	are	be	VBP
cord-014597-66vd2mdu	2258	16	commonly	commonly	RB
cord-014597-66vd2mdu	2258	17	used	use	VBN
cord-014597-66vd2mdu	2258	18	will	will	MD
cord-014597-66vd2mdu	2258	19	be	be	VB
cord-014597-66vd2mdu	2258	20	introduced	introduce	VBN
cord-014597-66vd2mdu	2258	21	.	.	.
cord-014597-66vd2mdu	2259	1	The	the	DT
cord-014597-66vd2mdu	2259	2	main	main	JJ
cord-014597-66vd2mdu	2259	3	idea	idea	NN
cord-014597-66vd2mdu	2259	4	is	be	VBZ
cord-014597-66vd2mdu	2259	5	to	to	TO
cord-014597-66vd2mdu	2259	6	give	give	VB
cord-014597-66vd2mdu	2259	7	access	access	NN
cord-014597-66vd2mdu	2259	8	to	to	IN
cord-014597-66vd2mdu	2259	9	automated	automate	VBN
cord-014597-66vd2mdu	2259	10	infrastructure	infrastructure	NN
cord-014597-66vd2mdu	2259	11	for	for	IN
cord-014597-66vd2mdu	2259	12	academic	academic	JJ
cord-014597-66vd2mdu	2259	13	research	research	NN
cord-014597-66vd2mdu	2259	14	or	or	CC
cord-014597-66vd2mdu	2259	15	spin	spin	NN
cord-014597-66vd2mdu	2259	16	-	-	HYPH
cord-014597-66vd2mdu	2259	17	offs	off	NNS
cord-014597-66vd2mdu	2259	18	which	which	WDT
cord-014597-66vd2mdu	2259	19	can	can	MD
cord-014597-66vd2mdu	2259	20	not	not	RB
cord-014597-66vd2mdu	2259	21	afford	afford	VB
cord-014597-66vd2mdu	2259	22	the	the	DT
cord-014597-66vd2mdu	2259	23	special	special	JJ
cord-014597-66vd2mdu	2259	24	infrastructure	infrastructure	NN
cord-014597-66vd2mdu	2259	25	.	.	.
cord-014597-66vd2mdu	2259	26	 	 	_SP
cord-014597-66vd2mdu	2260	1	Nowadays	nowadays	RB
cord-014597-66vd2mdu	2260	2	it	-PRON-	PRP
cord-014597-66vd2mdu	2260	3	is	be	VBZ
cord-014597-66vd2mdu	2260	4	known	know	VBN
cord-014597-66vd2mdu	2260	5	that	that	IN
cord-014597-66vd2mdu	2260	6	the	the	DT
cord-014597-66vd2mdu	2260	7	development	development	NN
cord-014597-66vd2mdu	2260	8	of	of	IN
cord-014597-66vd2mdu	2260	9	inhibitory	inhibitory	JJ
cord-014597-66vd2mdu	2260	10	antibodies	antibody	NNS
cord-014597-66vd2mdu	2260	11	by	by	IN
cord-014597-66vd2mdu	2260	12	hemophiliac	hemophiliac	NN
cord-014597-66vd2mdu	2260	13	patients	patient	NNS
cord-014597-66vd2mdu	2260	14	is	be	VBZ
cord-014597-66vd2mdu	2260	15	closely	closely	RB
cord-014597-66vd2mdu	2260	16	related	relate	VBN
cord-014597-66vd2mdu	2260	17	with	with	IN
cord-014597-66vd2mdu	2260	18	immunogenic	immunogenic	JJ
cord-014597-66vd2mdu	2260	19	epitopes	epitope	NNS
cord-014597-66vd2mdu	2260	20	present	present	JJ
cord-014597-66vd2mdu	2260	21	in	in	IN
cord-014597-66vd2mdu	2260	22	the	the	DT
cord-014597-66vd2mdu	2260	23	coagulation	coagulation	NN
cord-014597-66vd2mdu	2260	24	factors	factor	NNS
cord-014597-66vd2mdu	2260	25	.	.	.
cord-014597-66vd2mdu	2261	1	These	these	DT
cord-014597-66vd2mdu	2261	2	proteins	protein	NNS
cord-014597-66vd2mdu	2261	3	are	be	VBP
cord-014597-66vd2mdu	2261	4	produced	produce	VBN
cord-014597-66vd2mdu	2261	5	in	in	IN
cord-014597-66vd2mdu	2261	6	hamsters	hamster	NNS
cord-014597-66vd2mdu	2261	7	cells	cell	NNS
cord-014597-66vd2mdu	2261	8	[	[	-LRB-
cord-014597-66vd2mdu	2261	9	1	1	CD
cord-014597-66vd2mdu	2261	10	-4	-4	CD
cord-014597-66vd2mdu	2261	11	]	]	-RRB-
cord-014597-66vd2mdu	2261	12	which	which	WDT
cord-014597-66vd2mdu	2261	13	insert	insert	VBP
cord-014597-66vd2mdu	2261	14	a	a	DT
cord-014597-66vd2mdu	2261	15	different	different	JJ
cord-014597-66vd2mdu	2261	16	posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	2261	17	modification	modification	NN
cord-014597-66vd2mdu	2261	18	profile	profile	NN
cord-014597-66vd2mdu	2261	19	when	when	WRB
cord-014597-66vd2mdu	2261	20	compared	compare	VBN
cord-014597-66vd2mdu	2261	21	with	with	IN
cord-014597-66vd2mdu	2261	22	the	the	DT
cord-014597-66vd2mdu	2261	23	human	human	JJ
cord-014597-66vd2mdu	2261	24	profile	profile	NN
cord-014597-66vd2mdu	2261	25	.	.	.
cord-014597-66vd2mdu	2262	1	Patients	patient	NNS
cord-014597-66vd2mdu	2262	2	with	with	IN
cord-014597-66vd2mdu	2262	3	high	high	JJ
cord-014597-66vd2mdu	2262	4	-	-	HYPH
cord-014597-66vd2mdu	2262	5	titer	titer	NN
cord-014597-66vd2mdu	2262	6	/	/	SYM
cord-014597-66vd2mdu	2262	7	high	high	RB
cord-014597-66vd2mdu	2262	8	-	-	HYPH
cord-014597-66vd2mdu	2262	9	responding	respond	VBG
cord-014597-66vd2mdu	2262	10	inhibitors	inhibitor	NNS
cord-014597-66vd2mdu	2262	11	must	must	MD
cord-014597-66vd2mdu	2262	12	be	be	VB
cord-014597-66vd2mdu	2262	13	treated	treat	VBN
cord-014597-66vd2mdu	2262	14	with	with	IN
cord-014597-66vd2mdu	2262	15	bypassing	bypass	VBG
cord-014597-66vd2mdu	2262	16	agents	agent	NNS
cord-014597-66vd2mdu	2262	17	that	that	WDT
cord-014597-66vd2mdu	2262	18	can	can	MD
cord-014597-66vd2mdu	2262	19	achieve	achieve	VB
cord-014597-66vd2mdu	2262	20	hemostasis	hemostasis	NN
cord-014597-66vd2mdu	2262	21	.	.	.
cord-014597-66vd2mdu	2263	1	Activated	activate	VBN
cord-014597-66vd2mdu	2263	2	factor	factor	NN
cord-014597-66vd2mdu	2263	3	VII	vii	NN
cord-014597-66vd2mdu	2263	4	(	(	-LRB-
cord-014597-66vd2mdu	2263	5	FVIIa	FVIIa	NNS
cord-014597-66vd2mdu	2263	6	)	)	-RRB-
cord-014597-66vd2mdu	2263	7	is	be	VBZ
cord-014597-66vd2mdu	2263	8	an	an	DT
cord-014597-66vd2mdu	2263	9	attractive	attractive	JJ
cord-014597-66vd2mdu	2263	10	candidate	candidate	NN
cord-014597-66vd2mdu	2263	11	for	for	IN
cord-014597-66vd2mdu	2263	12	hemostasis	hemostasis	NN
cord-014597-66vd2mdu	2263	13	,	,	,
cord-014597-66vd2mdu	2263	14	independent	independent	JJ
cord-014597-66vd2mdu	2263	15	of	of	IN
cord-014597-66vd2mdu	2263	16	FVIII	FVIII	NNP
cord-014597-66vd2mdu	2263	17	/	/	SYM
cord-014597-66vd2mdu	2263	18	FIX	fix	NN
cord-014597-66vd2mdu	2263	19	,	,	,
cord-014597-66vd2mdu	2263	20	making	make	VBG
cord-014597-66vd2mdu	2263	21	this	this	DT
cord-014597-66vd2mdu	2263	22	coagulation	coagulation	NN
cord-014597-66vd2mdu	2263	23	factor	factor	NN
cord-014597-66vd2mdu	2263	24	an	an	DT
cord-014597-66vd2mdu	2263	25	alternative	alternative	NN
cord-014597-66vd2mdu	2263	26	for	for	IN
cord-014597-66vd2mdu	2263	27	hemophilia	hemophilia	NN
cord-014597-66vd2mdu	2263	28	patients	patient	NNS
cord-014597-66vd2mdu	2263	29	with	with	IN
cord-014597-66vd2mdu	2263	30	inhibitory	inhibitory	JJ
cord-014597-66vd2mdu	2263	31	antibodies	antibody	NNS
cord-014597-66vd2mdu	2263	32	.	.	.
cord-014597-66vd2mdu	2264	1	However	however	RB
cord-014597-66vd2mdu	2264	2	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2264	3	factor	factor	NN
cord-014597-66vd2mdu	2264	4	VII	VII	NNP
cord-014597-66vd2mdu	2264	5	is	be	VBZ
cord-014597-66vd2mdu	2264	6	produced	produce	VBN
cord-014597-66vd2mdu	2264	7	in	in	IN
cord-014597-66vd2mdu	2264	8	BHK-21	BHK-21	NNP
cord-014597-66vd2mdu	2264	9	cells	cell	NNS
cord-014597-66vd2mdu	2264	10	(	(	-LRB-
cord-014597-66vd2mdu	2264	11	Baby	Baby	NNP
cord-014597-66vd2mdu	2264	12	hamster	hamster	NN
cord-014597-66vd2mdu	2264	13	kidney	kidney	NN
cord-014597-66vd2mdu	2264	14	cells	cell	NNS
cord-014597-66vd2mdu	2264	15	)	)	-RRB-
cord-014597-66vd2mdu	2264	16	and	and	CC
cord-014597-66vd2mdu	2264	17	as	as	RB
cord-014597-66vd2mdu	2264	18	well	well	RB
cord-014597-66vd2mdu	2264	19	as	as	IN
cord-014597-66vd2mdu	2264	20	the	the	DT
cord-014597-66vd2mdu	2264	21	others	other	NNS
cord-014597-66vd2mdu	2264	22	coagulation	coagulation	NN
cord-014597-66vd2mdu	2264	23	factors	factor	NNS
cord-014597-66vd2mdu	2264	24	,	,	,
cord-014597-66vd2mdu	2264	25	it	-PRON-	PRP
cord-014597-66vd2mdu	2264	26	may	may	MD
cord-014597-66vd2mdu	2264	27	contain	contain	VB
cord-014597-66vd2mdu	2264	28	immunogenic	immunogenic	JJ
cord-014597-66vd2mdu	2264	29	epitopes	epitope	NNS
cord-014597-66vd2mdu	2264	30	[	[	-LRB-
cord-014597-66vd2mdu	2264	31	5	5	CD
cord-014597-66vd2mdu	2264	32	-7	-7	CD
cord-014597-66vd2mdu	2264	33	]	]	-RRB-
cord-014597-66vd2mdu	2264	34	.	.	.
cord-014597-66vd2mdu	2265	1	In	in	IN
cord-014597-66vd2mdu	2265	2	this	this	DT
cord-014597-66vd2mdu	2265	3	context	context	NN
cord-014597-66vd2mdu	2265	4	,	,	,
cord-014597-66vd2mdu	2265	5	becomes	become	VBZ
cord-014597-66vd2mdu	2265	6	extremely	extremely	RB
cord-014597-66vd2mdu	2265	7	important	important	JJ
cord-014597-66vd2mdu	2265	8	to	to	TO
cord-014597-66vd2mdu	2265	9	produce	produce	VB
cord-014597-66vd2mdu	2265	10	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2265	11	proteins	protein	NNS
cord-014597-66vd2mdu	2265	12	with	with	IN
cord-014597-66vd2mdu	2265	13	complex	complex	JJ
cord-014597-66vd2mdu	2265	14	posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	2265	15	modifications	modification	NNS
cord-014597-66vd2mdu	2265	16	in	in	IN
cord-014597-66vd2mdu	2265	17	a	a	DT
cord-014597-66vd2mdu	2265	18	cell	cell	NN
cord-014597-66vd2mdu	2265	19	line	line	NN
cord-014597-66vd2mdu	2265	20	not	not	RB
cord-014597-66vd2mdu	2265	21	yet	yet	RB
cord-014597-66vd2mdu	2265	22	used	use	VBN
cord-014597-66vd2mdu	2265	23	[	[	-LRB-
cord-014597-66vd2mdu	2265	24	8	8	CD
cord-014597-66vd2mdu	2265	25	-10	-10	NN
cord-014597-66vd2mdu	2265	26	]	]	-RRB-
cord-014597-66vd2mdu	2265	27	.	.	.
cord-014597-66vd2mdu	2266	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2266	2	have	have	VBP
cord-014597-66vd2mdu	2266	3	been	be	VBN
cord-014597-66vd2mdu	2266	4	using	use	VBG
cord-014597-66vd2mdu	2266	5	the	the	DT
cord-014597-66vd2mdu	2266	6	Sk	Sk	NNP
cord-014597-66vd2mdu	2266	7	-	-	HYPH
cord-014597-66vd2mdu	2266	8	Hep-1	Hep-1	NNP
cord-014597-66vd2mdu	2266	9	human	human	JJ
cord-014597-66vd2mdu	2266	10	cell	cell	NN
cord-014597-66vd2mdu	2266	11	line	line	NN
cord-014597-66vd2mdu	2266	12	for	for	IN
cord-014597-66vd2mdu	2266	13	the	the	DT
cord-014597-66vd2mdu	2266	14	production	production	NN
cord-014597-66vd2mdu	2266	15	of	of	IN
cord-014597-66vd2mdu	2266	16	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2266	17	FVII	FVII	NNP
cord-014597-66vd2mdu	2266	18	.	.	.
cord-014597-66vd2mdu	2267	1	To	to	TO
cord-014597-66vd2mdu	2267	2	generate	generate	VB
cord-014597-66vd2mdu	2267	3	the	the	DT
cord-014597-66vd2mdu	2267	4	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2267	5	cell	cell	NN
cord-014597-66vd2mdu	2267	6	line	line	NN
cord-014597-66vd2mdu	2267	7	we	-PRON-	PRP
cord-014597-66vd2mdu	2267	8	have	have	VBP
cord-014597-66vd2mdu	2267	9	used	use	VBN
cord-014597-66vd2mdu	2267	10	a	a	DT
cord-014597-66vd2mdu	2267	11	bicistronic	bicistronic	JJ
cord-014597-66vd2mdu	2267	12	lentiviral	lentiviral	JJ
cord-014597-66vd2mdu	2267	13	vector	vector	NN
cord-014597-66vd2mdu	2267	14	,	,	,
cord-014597-66vd2mdu	2267	15	1054-GFP	1054-gfp	CD
cord-014597-66vd2mdu	2267	16	,	,	,
cord-014597-66vd2mdu	2267	17	containing	contain	VBG
cord-014597-66vd2mdu	2267	18	a	a	DT
cord-014597-66vd2mdu	2267	19	FVII	FVII	NNP
cord-014597-66vd2mdu	2267	20	gene	gene	NN
cord-014597-66vd2mdu	2267	21	and	and	CC
cord-014597-66vd2mdu	2267	22	the	the	DT
cord-014597-66vd2mdu	2267	23	GFP	GFP	NNP
cord-014597-66vd2mdu	2267	24	selection	selection	NN
cord-014597-66vd2mdu	2267	25	marker	marker	NN
cord-014597-66vd2mdu	2267	26	gene	gene	NN
cord-014597-66vd2mdu	2267	27	.	.	.
cord-014597-66vd2mdu	2268	1	A	a	DT
cord-014597-66vd2mdu	2268	2	master	master	NN
cord-014597-66vd2mdu	2268	3	cell	cell	NN
cord-014597-66vd2mdu	2268	4	bank	bank	NN
cord-014597-66vd2mdu	2268	5	and	and	CC
cord-014597-66vd2mdu	2268	6	a	a	DT
cord-014597-66vd2mdu	2268	7	work	work	NN
cord-014597-66vd2mdu	2268	8	cell	cell	NN
cord-014597-66vd2mdu	2268	9	bank	bank	NN
cord-014597-66vd2mdu	2268	10	were	be	VBD
cord-014597-66vd2mdu	2268	11	generated	generate	VBN
cord-014597-66vd2mdu	2268	12	in	in	IN
cord-014597-66vd2mdu	2268	13	GMP	GMP	NNP
cord-014597-66vd2mdu	2268	14	conditions	condition	NNS
cord-014597-66vd2mdu	2268	15	.	.	.
cord-014597-66vd2mdu	2269	1	The	the	DT
cord-014597-66vd2mdu	2269	2	rFVII	rfvii	NN
cord-014597-66vd2mdu	2269	3	analyses	analysis	NNS
cord-014597-66vd2mdu	2269	4	were	be	VBD
cord-014597-66vd2mdu	2269	5	made	make	VBN
cord-014597-66vd2mdu	2269	6	by	by	IN
cord-014597-66vd2mdu	2269	7	ELISA	ELISA	NNP
cord-014597-66vd2mdu	2269	8	assay	assay	NN
cord-014597-66vd2mdu	2269	9	,	,	,
cord-014597-66vd2mdu	2269	10	western	western	JJ
cord-014597-66vd2mdu	2269	11	blot	blot	NN
cord-014597-66vd2mdu	2269	12	,	,	,
cord-014597-66vd2mdu	2269	13	gene	gene	NN
cord-014597-66vd2mdu	2269	14	expression	expression	NN
cord-014597-66vd2mdu	2269	15	quantification	quantification	NN
cord-014597-66vd2mdu	2269	16	and	and	CC
cord-014597-66vd2mdu	2269	17	biological	biological	JJ
cord-014597-66vd2mdu	2269	18	activity	activity	NN
cord-014597-66vd2mdu	2269	19	using	use	VBG
cord-014597-66vd2mdu	2269	20	the	the	DT
cord-014597-66vd2mdu	2269	21	Prothrombin	Prothrombin	NNP
cord-014597-66vd2mdu	2269	22	Time	Time	NNP
cord-014597-66vd2mdu	2269	23	(	(	-LRB-
cord-014597-66vd2mdu	2269	24	PT	PT	NNP
cord-014597-66vd2mdu	2269	25	)	)	-RRB-
cord-014597-66vd2mdu	2269	26	assay	assay	NN
cord-014597-66vd2mdu	2269	27	.	.	.
cord-014597-66vd2mdu	2270	1	rFVII	rFVII	NNP
cord-014597-66vd2mdu	2270	2	purification	purification	NN
cord-014597-66vd2mdu	2270	3	by	by	IN
cord-014597-66vd2mdu	2270	4	affinity	affinity	NN
cord-014597-66vd2mdu	2270	5	chromatography	chromatography	NN
cord-014597-66vd2mdu	2270	6	using	use	VBG
cord-014597-66vd2mdu	2270	7	VIISelect	VIISelect	NNP
cord-014597-66vd2mdu	2270	8	(	(	-LRB-
cord-014597-66vd2mdu	2270	9	GE	GE	NNP
cord-014597-66vd2mdu	2270	10	)	)	-RRB-
cord-014597-66vd2mdu	2270	11	column	column	NN
cord-014597-66vd2mdu	2270	12	.	.	.
cord-014597-66vd2mdu	2271	1	After	after	IN
cord-014597-66vd2mdu	2271	2	purification	purification	NN
cord-014597-66vd2mdu	2271	3	the	the	DT
cord-014597-66vd2mdu	2271	4	rFVII	rfvii	NN
cord-014597-66vd2mdu	2271	5	was	be	VBD
cord-014597-66vd2mdu	2271	6	formulated	formulate	VBN
cord-014597-66vd2mdu	2271	7	and	and	CC
cord-014597-66vd2mdu	2271	8	dry	dry	JJ
cord-014597-66vd2mdu	2271	9	froze	freeze	VBN
cord-014597-66vd2mdu	2271	10	to	to	TO
cord-014597-66vd2mdu	2271	11	be	be	VB
cord-014597-66vd2mdu	2271	12	used	use	VBN
cord-014597-66vd2mdu	2271	13	in	in	IN
cord-014597-66vd2mdu	2271	14	in	in	IN
cord-014597-66vd2mdu	2271	15	vivo	vivo	NN
cord-014597-66vd2mdu	2271	16	experiments	experiment	NNS
cord-014597-66vd2mdu	2271	17	.	.	.
cord-014597-66vd2mdu	2272	1	In	in	IN
cord-014597-66vd2mdu	2272	2	static	static	JJ
cord-014597-66vd2mdu	2272	3	conditions	condition	NNS
cord-014597-66vd2mdu	2272	4	Sk	Sk	NNP
cord-014597-66vd2mdu	2272	5	-	-	HYPH
cord-014597-66vd2mdu	2272	6	Hep-1	Hep-1	NNP
cord-014597-66vd2mdu	2272	7	cells	cell	NNS
cord-014597-66vd2mdu	2272	8	showed	show	VBD
cord-014597-66vd2mdu	2272	9	,	,	,
cord-014597-66vd2mdu	2272	10	for	for	IN
cord-014597-66vd2mdu	2272	11	a	a	DT
cord-014597-66vd2mdu	2272	12	period	period	NN
cord-014597-66vd2mdu	2272	13	of	of	IN
cord-014597-66vd2mdu	2272	14	6	6	CD
cord-014597-66vd2mdu	2272	15	months	month	NNS
cord-014597-66vd2mdu	2272	16	,	,	,
cord-014597-66vd2mdu	2272	17	a	a	DT
cord-014597-66vd2mdu	2272	18	stable	stable	JJ
cord-014597-66vd2mdu	2272	19	FVII	FVII	NNP
cord-014597-66vd2mdu	2272	20	production	production	NN
cord-014597-66vd2mdu	2272	21	with	with	IN
cord-014597-66vd2mdu	2272	22	an	an	DT
cord-014597-66vd2mdu	2272	23	average	average	NN
cord-014597-66vd2mdu	2272	24	of	of	IN
cord-014597-66vd2mdu	2272	25	8,03	8,03	CD
cord-014597-66vd2mdu	2272	26	IU	IU	NNP
cord-014597-66vd2mdu	2272	27	/	/	,
cord-014597-66vd2mdu	2272	28	mL	ml	NN
cord-014597-66vd2mdu	2272	29	of	of	IN
cord-014597-66vd2mdu	2272	30	FVII	FVII	NNP
cord-014597-66vd2mdu	2272	31	,	,	,
cord-014597-66vd2mdu	2272	32	83	83	CD
cord-014597-66vd2mdu	2272	33	%	%	NN
cord-014597-66vd2mdu	2272	34	of	of	IN
cord-014597-66vd2mdu	2272	35	cell	cell	NN
cord-014597-66vd2mdu	2272	36	viability	viability	NN
cord-014597-66vd2mdu	2272	37	and	and	CC
cord-014597-66vd2mdu	2272	38	77	77	CD
cord-014597-66vd2mdu	2272	39	%	%	NN
cord-014597-66vd2mdu	2272	40	of	of	IN
cord-014597-66vd2mdu	2272	41	cells	cell	NNS
cord-014597-66vd2mdu	2272	42	expressing	express	VBG
cord-014597-66vd2mdu	2272	43	the	the	DT
cord-014597-66vd2mdu	2272	44	GFP	GFP	NNP
cord-014597-66vd2mdu	2272	45	gene	gene	NN
cord-014597-66vd2mdu	2272	46	.	.	.
cord-014597-66vd2mdu	2273	1	After	after	IN
cord-014597-66vd2mdu	2273	2	purification	purification	NN
cord-014597-66vd2mdu	2273	3	with	with	IN
cord-014597-66vd2mdu	2273	4	VIISelect	viiselect	JJ
cord-014597-66vd2mdu	2273	5	column	column	NN
cord-014597-66vd2mdu	2273	6	it	-PRON-	PRP
cord-014597-66vd2mdu	2273	7	was	be	VBD
cord-014597-66vd2mdu	2273	8	possible	possible	JJ
cord-014597-66vd2mdu	2273	9	observe	observe	VB
cord-014597-66vd2mdu	2273	10	a	a	DT
cord-014597-66vd2mdu	2273	11	recover	recover	NN
cord-014597-66vd2mdu	2273	12	of	of	IN
cord-014597-66vd2mdu	2273	13	65	65	CD
cord-014597-66vd2mdu	2273	14	%	%	NN
cord-014597-66vd2mdu	2273	15	of	of	IN
cord-014597-66vd2mdu	2273	16	the	the	DT
cord-014597-66vd2mdu	2273	17	purified	purify	VBN
cord-014597-66vd2mdu	2273	18	protein	protein	NN
cord-014597-66vd2mdu	2273	19	with	with	IN
cord-014597-66vd2mdu	2273	20	95	95	CD
cord-014597-66vd2mdu	2273	21	%	%	NN
cord-014597-66vd2mdu	2273	22	degree	degree	NN
cord-014597-66vd2mdu	2273	23	of	of	IN
cord-014597-66vd2mdu	2273	24	purity	purity	NN
cord-014597-66vd2mdu	2273	25	(	(	-LRB-
cord-014597-66vd2mdu	2273	26	Fig	Fig	NNP
cord-014597-66vd2mdu	2273	27	.	.	.
cord-014597-66vd2mdu	2273	28	1	1	CD
cord-014597-66vd2mdu	2273	29	)	)	-RRB-
cord-014597-66vd2mdu	2273	30	.	.	.
cord-014597-66vd2mdu	2274	1	This	this	DT
cord-014597-66vd2mdu	2274	2	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2274	3	purified	purify	VBN
cord-014597-66vd2mdu	2274	4	FVII	FVII	NNP
cord-014597-66vd2mdu	2274	5	is	be	VBZ
cord-014597-66vd2mdu	2274	6	being	be	VBG
cord-014597-66vd2mdu	2274	7	used	use	VBN
cord-014597-66vd2mdu	2274	8	in	in	IN
cord-014597-66vd2mdu	2274	9	in	in	IN
cord-014597-66vd2mdu	2274	10	vivo	vivo	NN
cord-014597-66vd2mdu	2274	11	experiments	experiment	NNS
cord-014597-66vd2mdu	2274	12	to	to	TO
cord-014597-66vd2mdu	2274	13	determine	determine	VB
cord-014597-66vd2mdu	2274	14	the	the	DT
cord-014597-66vd2mdu	2274	15	pharmacokinetics	pharmacokinetic	NNS
cord-014597-66vd2mdu	2274	16	parameters	parameter	NNS
cord-014597-66vd2mdu	2274	17	and	and	CC
cord-014597-66vd2mdu	2274	18	to	to	TO
cord-014597-66vd2mdu	2274	19	evaluate	evaluate	VB
cord-014597-66vd2mdu	2274	20	the	the	DT
cord-014597-66vd2mdu	2274	21	posttranslation	posttranslation	NN
cord-014597-66vd2mdu	2274	22	modifications	modification	NNS
cord-014597-66vd2mdu	2274	23	profile	profile	RB
cord-014597-66vd2mdu	2274	24	.	.	.
cord-014597-66vd2mdu	2275	1	In	in	IN
cord-014597-66vd2mdu	2275	2	conclusion	conclusion	NN
cord-014597-66vd2mdu	2275	3	,	,	,
cord-014597-66vd2mdu	2275	4	this	this	DT
cord-014597-66vd2mdu	2275	5	study	study	NN
cord-014597-66vd2mdu	2275	6	reports	report	VBZ
cord-014597-66vd2mdu	2275	7	the	the	DT
cord-014597-66vd2mdu	2275	8	use	use	NN
cord-014597-66vd2mdu	2275	9	of	of	IN
cord-014597-66vd2mdu	2275	10	Sk	Sk	NNP
cord-014597-66vd2mdu	2275	11	-	-	HYPH
cord-014597-66vd2mdu	2275	12	Hep-1	Hep-1	NNP
cord-014597-66vd2mdu	2275	13	cell	cell	NN
cord-014597-66vd2mdu	2275	14	line	line	NN
cord-014597-66vd2mdu	2275	15	for	for	IN
cord-014597-66vd2mdu	2275	16	high	high	JJ
cord-014597-66vd2mdu	2275	17	-	-	HYPH
cord-014597-66vd2mdu	2275	18	level	level	NN
cord-014597-66vd2mdu	2275	19	production	production	NN
cord-014597-66vd2mdu	2275	20	of	of	IN
cord-014597-66vd2mdu	2275	21	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2275	22	factor	factor	NN
cord-014597-66vd2mdu	2275	23	VII	VII	NNP
cord-014597-66vd2mdu	2275	24	.	.	.
cord-014597-66vd2mdu	2276	1	These	these	DT
cord-014597-66vd2mdu	2276	2	cells	cell	NNS
cord-014597-66vd2mdu	2276	3	have	have	VBP
cord-014597-66vd2mdu	2276	4	proven	prove	VBN
cord-014597-66vd2mdu	2276	5	to	to	TO
cord-014597-66vd2mdu	2276	6	be	be	VB
cord-014597-66vd2mdu	2276	7	effective	effective	JJ
cord-014597-66vd2mdu	2276	8	in	in	IN
cord-014597-66vd2mdu	2276	9	the	the	DT
cord-014597-66vd2mdu	2276	10	production	production	NN
cord-014597-66vd2mdu	2276	11	of	of	IN
cord-014597-66vd2mdu	2276	12	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2276	13	protein	protein	NN
cord-014597-66vd2mdu	2276	14	and	and	CC
cord-014597-66vd2mdu	2276	15	can	can	MD
cord-014597-66vd2mdu	2276	16	be	be	VB
cord-014597-66vd2mdu	2276	17	used	use	VBN
cord-014597-66vd2mdu	2276	18	as	as	IN
cord-014597-66vd2mdu	2276	19	a	a	DT
cord-014597-66vd2mdu	2276	20	new	new	JJ
cord-014597-66vd2mdu	2276	21	platform	platform	NN
cord-014597-66vd2mdu	2276	22	for	for	IN
cord-014597-66vd2mdu	2276	23	the	the	DT
cord-014597-66vd2mdu	2276	24	production	production	NN
cord-014597-66vd2mdu	2276	25	of	of	IN
cord-014597-66vd2mdu	2276	26	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2276	27	proteins	protein	NNS
cord-014597-66vd2mdu	2276	28	.	.	.
cord-014597-66vd2mdu	2277	1	Fig	Fig	NNP
cord-014597-66vd2mdu	2277	2	.	.	.
cord-014597-66vd2mdu	2277	3	1	1	CD
cord-014597-66vd2mdu	2277	4	(	(	-LRB-
cord-014597-66vd2mdu	2277	5	abstract	abstract	NNP
cord-014597-66vd2mdu	2277	6	P-366	P-366	NNP
cord-014597-66vd2mdu	2277	7	)	)	-RRB-
cord-014597-66vd2mdu	2277	8	.	.	.
cord-014597-66vd2mdu	2278	1	a	a	DT
cord-014597-66vd2mdu	2278	2	Determination	determination	NN
cord-014597-66vd2mdu	2278	3	of	of	IN
cord-014597-66vd2mdu	2278	4	protein	protein	NN
cord-014597-66vd2mdu	2278	5	yield	yield	NN
cord-014597-66vd2mdu	2278	6	of	of	IN
cord-014597-66vd2mdu	2278	7	EGFR	EGFR	NNP
cord-014597-66vd2mdu	2278	8	(	(	-LRB-
cord-014597-66vd2mdu	2278	9	Epidermal	epidermal	JJ
cord-014597-66vd2mdu	2278	10	growth	growth	NN
cord-014597-66vd2mdu	2278	11	factor	factor	NN
cord-014597-66vd2mdu	2278	12	receptor	receptor	NN
cord-014597-66vd2mdu	2278	13	)	)	-RRB-
cord-014597-66vd2mdu	2278	14	synthesized	synthesize	VBN
cord-014597-66vd2mdu	2278	15	in	in	IN
cord-014597-66vd2mdu	2278	16	a	a	DT
cord-014597-66vd2mdu	2278	17	CHO	CHO	NNP
cord-014597-66vd2mdu	2278	18	CECF	CECF	NNP
cord-014597-66vd2mdu	2278	19	system	system	NN
cord-014597-66vd2mdu	2278	20	.	.	.
cord-014597-66vd2mdu	2279	1	Analysis	analysis	NN
cord-014597-66vd2mdu	2279	2	of	of	IN
cord-014597-66vd2mdu	2279	3	EGFR	EGFR	NNP
cord-014597-66vd2mdu	2279	4	protein	protein	NN
cord-014597-66vd2mdu	2279	5	yield	yield	NN
cord-014597-66vd2mdu	2279	6	obtained	obtain	VBN
cord-014597-66vd2mdu	2279	7	in	in	IN
cord-014597-66vd2mdu	2279	8	a	a	DT
cord-014597-66vd2mdu	2279	9	various	various	JJ
cord-014597-66vd2mdu	2279	10	batches	batch	NNS
cord-014597-66vd2mdu	2279	11	of	of	IN
cord-014597-66vd2mdu	2279	12	CECF	CECF	NNP
cord-014597-66vd2mdu	2279	13	formatted	format	VBN
cord-014597-66vd2mdu	2279	14	reaction	reaction	NN
cord-014597-66vd2mdu	2279	15	.	.	.
cord-014597-66vd2mdu	2280	1	CECF	CECF	NNP
cord-014597-66vd2mdu	2280	2	synthesis	synthesis	NN
cord-014597-66vd2mdu	2280	3	was	be	VBD
cord-014597-66vd2mdu	2280	4	performed	perform	VBN
cord-014597-66vd2mdu	2280	5	in	in	IN
cord-014597-66vd2mdu	2280	6	the	the	DT
cord-014597-66vd2mdu	2280	7	presence	presence	NN
cord-014597-66vd2mdu	2280	8	of	of	IN
cord-014597-66vd2mdu	2280	9	14	14	CD
cord-014597-66vd2mdu	2280	10	C	c	NN
cord-014597-66vd2mdu	2280	11	leucine	leucine	NN
cord-014597-66vd2mdu	2280	12	for	for	IN
cord-014597-66vd2mdu	2280	13	radio	radio	NN
cord-014597-66vd2mdu	2280	14	labeling	labeling	NN
cord-014597-66vd2mdu	2280	15	of	of	IN
cord-014597-66vd2mdu	2280	16	target	target	NN
cord-014597-66vd2mdu	2280	17	proteins	protein	NNS
cord-014597-66vd2mdu	2280	18	.	.	.
cord-014597-66vd2mdu	2281	1	Radio	radio	NN
cord-014597-66vd2mdu	2281	2	labeled	label	VBD
cord-014597-66vd2mdu	2281	3	proteins	protein	NNS
cord-014597-66vd2mdu	2281	4	were	be	VBD
cord-014597-66vd2mdu	2281	5	precipitated	precipitate	VBN
cord-014597-66vd2mdu	2281	6	using	use	VBG
cord-014597-66vd2mdu	2281	7	TCA	TCA	NNP
cord-014597-66vd2mdu	2281	8	followed	follow	VBN
cord-014597-66vd2mdu	2281	9	by	by	IN
cord-014597-66vd2mdu	2281	10	scintillation	scintillation	NN
cord-014597-66vd2mdu	2281	11	measurement	measurement	NN
cord-014597-66vd2mdu	2281	12	.	.	.
cord-014597-66vd2mdu	2282	1	b	b	LS
cord-014597-66vd2mdu	2282	2	Detection	detection	NN
cord-014597-66vd2mdu	2282	3	of	of	IN
cord-014597-66vd2mdu	2282	4	radio	radio	NN
cord-014597-66vd2mdu	2282	5	labeled	label	VBN
cord-014597-66vd2mdu	2282	6	EGFR	EGFR	NNP
cord-014597-66vd2mdu	2282	7	by	by	IN
cord-014597-66vd2mdu	2282	8	autoradiography	autoradiography	NNP
cord-014597-66vd2mdu	2282	9	.	.	.
cord-014597-66vd2mdu	2283	1	A	a	DT
cord-014597-66vd2mdu	2283	2	no	no	DT
cord-014597-66vd2mdu	2283	3	template	template	NN
cord-014597-66vd2mdu	2283	4	control	control	NN
cord-014597-66vd2mdu	2283	5	(	(	-LRB-
cord-014597-66vd2mdu	2283	6	NTC	NTC	NNP
cord-014597-66vd2mdu	2283	7	)	)	-RRB-
cord-014597-66vd2mdu	2283	8	was	be	VBD
cord-014597-66vd2mdu	2283	9	prepared	prepare	VBN
cord-014597-66vd2mdu	2283	10	containing	contain	VBG
cord-014597-66vd2mdu	2283	11	no	no	DT
cord-014597-66vd2mdu	2283	12	EGFR	EGFR	NNP
cord-014597-66vd2mdu	2283	13	DNA	dna	NN
cord-014597-66vd2mdu	2283	14	template	template	NN
cord-014597-66vd2mdu	2283	15	Background	Background	NNP
cord-014597-66vd2mdu	2283	16	Emergence	Emergence	NNP
cord-014597-66vd2mdu	2283	17	of	of	IN
cord-014597-66vd2mdu	2283	18	stem	stem	NN
cord-014597-66vd2mdu	2283	19	cell	cell	NN
cord-014597-66vd2mdu	2283	20	-	-	HYPH
cord-014597-66vd2mdu	2283	21	based	base	VBN
cord-014597-66vd2mdu	2283	22	regenerative	regenerative	JJ
cord-014597-66vd2mdu	2283	23	medicine	medicine	NN
cord-014597-66vd2mdu	2283	24	recently	recently	RB
cord-014597-66vd2mdu	2283	25	leaded	lead	VBD
cord-014597-66vd2mdu	2283	26	to	to	IN
cord-014597-66vd2mdu	2283	27	the	the	DT
cord-014597-66vd2mdu	2283	28	necessity	necessity	NN
cord-014597-66vd2mdu	2283	29	to	to	TO
cord-014597-66vd2mdu	2283	30	reach	reach	VB
cord-014597-66vd2mdu	2283	31	a	a	DT
cord-014597-66vd2mdu	2283	32	sustained	sustained	JJ
cord-014597-66vd2mdu	2283	33	production	production	NN
cord-014597-66vd2mdu	2283	34	of	of	IN
cord-014597-66vd2mdu	2283	35	such	such	JJ
cord-014597-66vd2mdu	2283	36	cells	cell	NNS
cord-014597-66vd2mdu	2283	37	[	[	-LRB-
cord-014597-66vd2mdu	2283	38	1	1	CD
cord-014597-66vd2mdu	2283	39	]	]	-RRB-
cord-014597-66vd2mdu	2283	40	.	.	.
cord-014597-66vd2mdu	2284	1	Hence	hence	RB
cord-014597-66vd2mdu	2284	2	,	,	,
cord-014597-66vd2mdu	2284	3	new	new	JJ
cord-014597-66vd2mdu	2284	4	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	2284	5	and	and	CC
cord-014597-66vd2mdu	2284	6	carriers	carrier	NNS
cord-014597-66vd2mdu	2284	7	were	be	VBD
cord-014597-66vd2mdu	2284	8	designed	design	VBN
cord-014597-66vd2mdu	2284	9	for	for	IN
cord-014597-66vd2mdu	2284	10	cell	cell	NN
cord-014597-66vd2mdu	2284	11	expansion	expansion	NN
cord-014597-66vd2mdu	2284	12	.	.	.
cord-014597-66vd2mdu	2285	1	However	however	RB
cord-014597-66vd2mdu	2285	2	,	,	,
cord-014597-66vd2mdu	2285	3	to	to	TO
cord-014597-66vd2mdu	2285	4	meet	meet	VB
cord-014597-66vd2mdu	2285	5	this	this	DT
cord-014597-66vd2mdu	2285	6	increasing	increase	VBG
cord-014597-66vd2mdu	2285	7	demand	demand	NN
cord-014597-66vd2mdu	2285	8	,	,	,
cord-014597-66vd2mdu	2285	9	improvement	improvement	NN
cord-014597-66vd2mdu	2285	10	of	of	IN
cord-014597-66vd2mdu	2285	11	both	both	CC
cord-014597-66vd2mdu	2285	12	quality	quality	NN
cord-014597-66vd2mdu	2285	13	and	and	CC
cord-014597-66vd2mdu	2285	14	quantity	quantity	NN
cord-014597-66vd2mdu	2285	15	of	of	IN
cord-014597-66vd2mdu	2285	16	stem	stem	NN
cord-014597-66vd2mdu	2285	17	cells	cell	NNS
cord-014597-66vd2mdu	2285	18	remains	remain	VBZ
cord-014597-66vd2mdu	2285	19	necessary	necessary	JJ
cord-014597-66vd2mdu	2285	20	.	.	.
cord-014597-66vd2mdu	2286	1	Soft	soft	JJ
cord-014597-66vd2mdu	2286	2	biocompatible	biocompatible	JJ
cord-014597-66vd2mdu	2286	3	microcarriers	microcarrier	NNS
cord-014597-66vd2mdu	2286	4	mimicking	mimic	VBG
cord-014597-66vd2mdu	2286	5	extracellular	extracellular	JJ
cord-014597-66vd2mdu	2286	6	matrix	matrix	NN
cord-014597-66vd2mdu	2286	7	in	in	IN
cord-014597-66vd2mdu	2286	8	term	term	NN
cord-014597-66vd2mdu	2286	9	of	of	IN
cord-014597-66vd2mdu	2286	10	structure	structure	NN
cord-014597-66vd2mdu	2286	11	and	and	CC
cord-014597-66vd2mdu	2286	12	stiffness	stiffness	NN
cord-014597-66vd2mdu	2286	13	should	should	MD
cord-014597-66vd2mdu	2286	14	be	be	VB
cord-014597-66vd2mdu	2286	15	of	of	IN
cord-014597-66vd2mdu	2286	16	valuable	valuable	JJ
cord-014597-66vd2mdu	2286	17	utility	utility	NN
cord-014597-66vd2mdu	2286	18	as	as	IN
cord-014597-66vd2mdu	2286	19	substrate	substrate	NN
cord-014597-66vd2mdu	2286	20	stiffness	stiffness	NN
cord-014597-66vd2mdu	2286	21	strongly	strongly	RB
cord-014597-66vd2mdu	2286	22	influence	influence	VBP
cord-014597-66vd2mdu	2286	23	in	in	IN
cord-014597-66vd2mdu	2286	24	vitro	vitro	FW
cord-014597-66vd2mdu	2286	25	stem	stem	NN
cord-014597-66vd2mdu	2286	26	cell	cell	NN
cord-014597-66vd2mdu	2286	27	fate	fate	NN
cord-014597-66vd2mdu	2286	28	and	and	CC
cord-014597-66vd2mdu	2286	29	differentiation	differentiation	NN
cord-014597-66vd2mdu	2286	30	[	[	-LRB-
cord-014597-66vd2mdu	2286	31	2	2	CD
cord-014597-66vd2mdu	2286	32	,	,	,
cord-014597-66vd2mdu	2286	33	3	3	CD
cord-014597-66vd2mdu	2286	34	]	]	-RRB-
cord-014597-66vd2mdu	2286	35	.	.	.
cord-014597-66vd2mdu	2287	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	2287	2	expertise	expertise	NN
cord-014597-66vd2mdu	2287	3	in	in	IN
cord-014597-66vd2mdu	2287	4	the	the	DT
cord-014597-66vd2mdu	2287	5	field	field	NN
cord-014597-66vd2mdu	2287	6	of	of	IN
cord-014597-66vd2mdu	2287	7	microbeads	microbead	NNS
cord-014597-66vd2mdu	2287	8	design	design	NN
cord-014597-66vd2mdu	2287	9	using	use	VBG
cord-014597-66vd2mdu	2287	10	jetcutting	jetcutte	VBG
cord-014597-66vd2mdu	2287	11	technology	technology	NN
cord-014597-66vd2mdu	2287	12	[	[	-LRB-
cord-014597-66vd2mdu	2287	13	4	4	CD
cord-014597-66vd2mdu	2287	14	]	]	-RRB-
cord-014597-66vd2mdu	2287	15	enabled	enable	VBD
cord-014597-66vd2mdu	2287	16	us	-PRON-	PRP
cord-014597-66vd2mdu	2287	17	to	to	TO
cord-014597-66vd2mdu	2287	18	engineer	engineer	VB
cord-014597-66vd2mdu	2287	19	+	+	CC
cord-014597-66vd2mdu	2287	20	/-200	/-200	.
cord-014597-66vd2mdu	2287	21	μm	μm	NN
cord-014597-66vd2mdu	2287	22	alginate	alginate	NN
cord-014597-66vd2mdu	2287	23	beads	bead	NNS
cord-014597-66vd2mdu	2287	24	of	of	IN
cord-014597-66vd2mdu	2287	25	various	various	JJ
cord-014597-66vd2mdu	2287	26	G	g	NN
cord-014597-66vd2mdu	2287	27	/	/	SYM
cord-014597-66vd2mdu	2287	28	M	M	NNP
cord-014597-66vd2mdu	2287	29	monomer	monomer	NN
cord-014597-66vd2mdu	2287	30	ratio	ratio	NN
cord-014597-66vd2mdu	2287	31	.	.	.
cord-014597-66vd2mdu	2288	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2288	2	used	use	VBD
cord-014597-66vd2mdu	2288	3	jetcutter	jetcutter	NNP
cord-014597-66vd2mdu	2288	4	(	(	-LRB-
cord-014597-66vd2mdu	2288	5	geniaLab	geniaLab	NNP
cord-014597-66vd2mdu	2288	6	Gmbh	Gmbh	NNP
cord-014597-66vd2mdu	2288	7	)	)	-RRB-
cord-014597-66vd2mdu	2288	8	with	with	IN
cord-014597-66vd2mdu	2288	9	100	100	CD
cord-014597-66vd2mdu	2288	10	μm	μm	NN
cord-014597-66vd2mdu	2288	11	nozzle	nozzle	NN
cord-014597-66vd2mdu	2288	12	at	at	IN
cord-014597-66vd2mdu	2288	13	max	max	NN
cord-014597-66vd2mdu	2288	14	speed	speed	NN
cord-014597-66vd2mdu	2288	15	12000	12000	CD
cord-014597-66vd2mdu	2288	16	rpm	rpm	NN
cord-014597-66vd2mdu	2288	17	.	.	.
cord-014597-66vd2mdu	2289	1	Alginate	alginate	NN
cord-014597-66vd2mdu	2289	2	solutions	solution	NNS
cord-014597-66vd2mdu	2289	3	with	with	IN
cord-014597-66vd2mdu	2289	4	concentrations	concentration	NNS
cord-014597-66vd2mdu	2289	5	2	2	CD
cord-014597-66vd2mdu	2289	6	%	%	NN
cord-014597-66vd2mdu	2289	7	to	to	TO
cord-014597-66vd2mdu	2289	8	4	4	CD
cord-014597-66vd2mdu	2289	9	%	%	NN
cord-014597-66vd2mdu	2289	10	were	be	VBD
cord-014597-66vd2mdu	2289	11	gelifyed	gelifye	VBN
cord-014597-66vd2mdu	2289	12	in	in	IN
cord-014597-66vd2mdu	2289	13	2	2	CD
cord-014597-66vd2mdu	2289	14	%	%	NN
cord-014597-66vd2mdu	2289	15	CaCl2	cacl2	NN
cord-014597-66vd2mdu	2289	16	EtOH	EtOH	NNP
cord-014597-66vd2mdu	2289	17	50	50	CD
cord-014597-66vd2mdu	2289	18	%	%	NN
cord-014597-66vd2mdu	2289	19	solution	solution	NN
cord-014597-66vd2mdu	2289	20	.	.	.
cord-014597-66vd2mdu	2290	1	Alginates	Alginates	NNP
cord-014597-66vd2mdu	2290	2	with	with	IN
cord-014597-66vd2mdu	2290	3	estimated	estimate	VBN
cord-014597-66vd2mdu	2290	4	viscosity	viscosity	NN
cord-014597-66vd2mdu	2290	5	(	(	-LRB-
cord-014597-66vd2mdu	2290	6	@1	@1	:
cord-014597-66vd2mdu	2290	7	%	%	NN
cord-014597-66vd2mdu	2290	8	)	)	-RRB-
cord-014597-66vd2mdu	2290	9	from	from	IN
cord-014597-66vd2mdu	2290	10	30	30	CD
cord-014597-66vd2mdu	2290	11	to	to	IN
cord-014597-66vd2mdu	2290	12	720	720	CD
cord-014597-66vd2mdu	2290	13	mPa	mPa	NNS
cord-014597-66vd2mdu	2290	14	were	be	VBD
cord-014597-66vd2mdu	2290	15	tested	test	VBN
cord-014597-66vd2mdu	2290	16	.	.	.
cord-014597-66vd2mdu	2291	1	A	a	DT
cord-014597-66vd2mdu	2291	2	further	further	JJ
cord-014597-66vd2mdu	2291	3	surface	surface	NN
cord-014597-66vd2mdu	2291	4	treatment	treatment	NN
cord-014597-66vd2mdu	2291	5	with	with	IN
cord-014597-66vd2mdu	2291	6	gelatine	gelatine	NN
cord-014597-66vd2mdu	2291	7	(	(	-LRB-
cord-014597-66vd2mdu	2291	8	0,1	0,1	CD
cord-014597-66vd2mdu	2291	9	%	%	NN
cord-014597-66vd2mdu	2291	10	,	,	,
cord-014597-66vd2mdu	2291	11	1	1	CD
cord-014597-66vd2mdu	2291	12	%	%	NN
cord-014597-66vd2mdu	2291	13	)	)	-RRB-
cord-014597-66vd2mdu	2291	14	and	and	CC
cord-014597-66vd2mdu	2291	15	poly	poly	NNP
cord-014597-66vd2mdu	2291	16	-	-	HYPH
cord-014597-66vd2mdu	2291	17	L	l	NN
cord-014597-66vd2mdu	2291	18	-	-	HYPH
cord-014597-66vd2mdu	2291	19	lysine	lysine	NN
cord-014597-66vd2mdu	2291	20	(	(	-LRB-
cord-014597-66vd2mdu	2291	21	0,1	0,1	CD
cord-014597-66vd2mdu	2291	22	%	%	NN
cord-014597-66vd2mdu	2291	23	)	)	-RRB-
cord-014597-66vd2mdu	2291	24	was	be	VBD
cord-014597-66vd2mdu	2291	25	carried	carry	VBN
cord-014597-66vd2mdu	2291	26	out	out	RP
cord-014597-66vd2mdu	2291	27	to	to	TO
cord-014597-66vd2mdu	2291	28	reach	reach	VB
cord-014597-66vd2mdu	2291	29	an	an	DT
cord-014597-66vd2mdu	2291	30	optimal	optimal	JJ
cord-014597-66vd2mdu	2291	31	cell	cell	NN
cord-014597-66vd2mdu	2291	32	anchoring	anchoring	NN
cord-014597-66vd2mdu	2291	33	of	of	IN
cord-014597-66vd2mdu	2291	34	human	human	JJ
cord-014597-66vd2mdu	2291	35	adiposederived	adiposederive	VBN
cord-014597-66vd2mdu	2291	36	mesenchymal	mesenchymal	JJ
cord-014597-66vd2mdu	2291	37	stem	stem	NN
cord-014597-66vd2mdu	2291	38	cells	cell	NNS
cord-014597-66vd2mdu	2291	39	(	(	-LRB-
cord-014597-66vd2mdu	2291	40	ATCC	ATCC	NNP
cord-014597-66vd2mdu	2291	41	-	-	HYPH
cord-014597-66vd2mdu	2291	42	PSC-500	PSC-500	NNP
cord-014597-66vd2mdu	2291	43	-	-	HYPH
cord-014597-66vd2mdu	2291	44	011	011	CD
cord-014597-66vd2mdu	2291	45	)	)	-RRB-
cord-014597-66vd2mdu	2291	46	in	in	IN
cord-014597-66vd2mdu	2291	47	MesemPro	MesemPro	NNP
cord-014597-66vd2mdu	2291	48	RS	rs	NN
cord-014597-66vd2mdu	2291	49	medium	medium	NN
cord-014597-66vd2mdu	2291	50	(	(	-LRB-
cord-014597-66vd2mdu	2291	51	Gibco	Gibco	NNP
cord-014597-66vd2mdu	2291	52	)	)	-RRB-
cord-014597-66vd2mdu	2291	53	.	.	.
cord-014597-66vd2mdu	2292	1	Jetcutter	Jetcutter	NNP
cord-014597-66vd2mdu	2292	2	technology	technology	NN
cord-014597-66vd2mdu	2292	3	allowed	allow	VBD
cord-014597-66vd2mdu	2292	4	us	-PRON-	PRP
cord-014597-66vd2mdu	2292	5	to	to	TO
cord-014597-66vd2mdu	2292	6	obtain	obtain	VB
cord-014597-66vd2mdu	2292	7	alginate	alginate	NN
cord-014597-66vd2mdu	2292	8	microcarriers	microcarrier	NNS
cord-014597-66vd2mdu	2292	9	with	with	IN
cord-014597-66vd2mdu	2292	10	a	a	DT
cord-014597-66vd2mdu	2292	11	good	good	JJ
cord-014597-66vd2mdu	2292	12	homogeneity	homogeneity	NN
cord-014597-66vd2mdu	2292	13	in	in	IN
cord-014597-66vd2mdu	2292	14	size	size	NN
cord-014597-66vd2mdu	2292	15	around	around	IN
cord-014597-66vd2mdu	2292	16	200	200	CD
cord-014597-66vd2mdu	2292	17	μm	μm	NNS
cord-014597-66vd2mdu	2292	18	and	and	CC
cord-014597-66vd2mdu	2292	19	sphericity	sphericity	NN
cord-014597-66vd2mdu	2292	20	comparable	comparable	JJ
cord-014597-66vd2mdu	2292	21	to	to	IN
cord-014597-66vd2mdu	2292	22	commercial	commercial	JJ
cord-014597-66vd2mdu	2292	23	carriers	carrier	NNS
cord-014597-66vd2mdu	2292	24	(	(	-LRB-
cord-014597-66vd2mdu	2292	25	Table	table	NN
cord-014597-66vd2mdu	2292	26	1	1	CD
cord-014597-66vd2mdu	2292	27	)	)	-RRB-
cord-014597-66vd2mdu	2292	28	.	.	.
cord-014597-66vd2mdu	2293	1	Best	good	JJS
cord-014597-66vd2mdu	2293	2	adhesion	adhesion	NN
cord-014597-66vd2mdu	2293	3	of	of	IN
cord-014597-66vd2mdu	2293	4	human	human	JJ
cord-014597-66vd2mdu	2293	5	adipose	adipose	NN
cord-014597-66vd2mdu	2293	6	-	-	HYPH
cord-014597-66vd2mdu	2293	7	derived	derive	VBN
cord-014597-66vd2mdu	2293	8	mesenchymal	mesenchymal	JJ
cord-014597-66vd2mdu	2293	9	stem	stem	NN
cord-014597-66vd2mdu	2293	10	cells	cell	NNS
cord-014597-66vd2mdu	2293	11	was	be	VBD
cord-014597-66vd2mdu	2293	12	obtained	obtain	VBN
cord-014597-66vd2mdu	2293	13	on	on	IN
cord-014597-66vd2mdu	2293	14	0,1	0,1	CD
cord-014597-66vd2mdu	2293	15	%	%	NN
cord-014597-66vd2mdu	2293	16	gelatine	gelatine	NN
cord-014597-66vd2mdu	2293	17	coated	coat	VBN
cord-014597-66vd2mdu	2293	18	alginate	alginate	NN
cord-014597-66vd2mdu	2293	19	carriers	carrier	NNS
cord-014597-66vd2mdu	2293	20	(	(	-LRB-
cord-014597-66vd2mdu	2293	21	Fig	fig	NN
cord-014597-66vd2mdu	2293	22	.	.	NNP
cord-014597-66vd2mdu	2293	23	1	1	CD
cord-014597-66vd2mdu	2293	24	)	)	-RRB-
cord-014597-66vd2mdu	2293	25	.	.	.
cord-014597-66vd2mdu	2294	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2294	2	observed	observe	VBD
cord-014597-66vd2mdu	2294	3	limited	limited	JJ
cord-014597-66vd2mdu	2294	4	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	2294	5	and	and	CC
cord-014597-66vd2mdu	2294	6	human	human	JJ
cord-014597-66vd2mdu	2294	7	adipose	adipose	NN
cord-014597-66vd2mdu	2294	8	-	-	HYPH
cord-014597-66vd2mdu	2294	9	derived	derive	VBN
cord-014597-66vd2mdu	2294	10	mesenchymal	mesenchymal	JJ
cord-014597-66vd2mdu	2294	11	cells	cell	NNS
cord-014597-66vd2mdu	2294	12	stemness	stemness	JJ
cord-014597-66vd2mdu	2294	13	was	be	VBD
cord-014597-66vd2mdu	2294	14	conserved	conserve	VBN
cord-014597-66vd2mdu	2294	15	after	after	IN
cord-014597-66vd2mdu	2294	16	14	14	CD
cord-014597-66vd2mdu	2294	17	days	day	NNS
cord-014597-66vd2mdu	2294	18	in	in	IN
cord-014597-66vd2mdu	2294	19	culture	culture	NN
cord-014597-66vd2mdu	2294	20	(	(	-LRB-
cord-014597-66vd2mdu	2294	21	data	datum	NNS
cord-014597-66vd2mdu	2294	22	not	not	RB
cord-014597-66vd2mdu	2294	23	shown	show	VBN
cord-014597-66vd2mdu	2294	24	)	)	-RRB-
cord-014597-66vd2mdu	2294	25	.	.	.
cord-014597-66vd2mdu	2295	1	Cellular	cellular	JJ
cord-014597-66vd2mdu	2295	2	bioassays	bioassay	NNS
cord-014597-66vd2mdu	2295	3	developed	develop	VBD
cord-014597-66vd2mdu	2295	4	with	with	IN
cord-014597-66vd2mdu	2295	5	functionally	functionally	RB
cord-014597-66vd2mdu	2295	6	immortalized	immortalize	VBN
cord-014597-66vd2mdu	2295	7	cell	cell	NN
cord-014597-66vd2mdu	2295	8	lines	line	NNS
cord-014597-66vd2mdu	2295	9	Aileen	Aileen	NNP
cord-014597-66vd2mdu	2295	10	Bleisch	Bleisch	NNP
cord-014597-66vd2mdu	2295	11	1	1	CD
cord-014597-66vd2mdu	2295	12	,	,	,
cord-014597-66vd2mdu	2295	13	Aleksandra	Aleksandra	NNP
cord-014597-66vd2mdu	2295	14	Velkova	Velkova	NNP
cord-014597-66vd2mdu	2295	15	3	3	CD
cord-014597-66vd2mdu	2295	16	,	,	,
cord-014597-66vd2mdu	2295	17	Tom	Tom	NNP
cord-014597-66vd2mdu	2295	18	Wahlicht	Wahlicht	NNP
cord-014597-66vd2mdu	2295	19	2	2	CD
cord-014597-66vd2mdu	2295	20	,	,	,
cord-014597-66vd2mdu	2295	21	Dagmar	Dagmar	NNP
cord-014597-66vd2mdu	2295	22	Wirth	Wirth	NNP
cord-014597-66vd2mdu	2295	23	2	2	CD
cord-014597-66vd2mdu	2295	24	,	,	,
cord-014597-66vd2mdu	2295	25	Tobias	Tobias	NNP
cord-014597-66vd2mdu	2295	26	May	May	NNP
cord-014597-66vd2mdu	2295	27	1	1	CD
cord-014597-66vd2mdu	2295	28	1	1	CD
cord-014597-66vd2mdu	2295	29	InSCREENeX	inscreenex	NN
cord-014597-66vd2mdu	2295	30	GmbH	GmbH	NNP
cord-014597-66vd2mdu	2295	31	,	,	,
cord-014597-66vd2mdu	2295	32	Braunschweig	Braunschweig	NNP
cord-014597-66vd2mdu	2295	33	,	,	,
cord-014597-66vd2mdu	2295	34	Germany	Germany	NNP
cord-014597-66vd2mdu	2295	35	;	;	:
cord-014597-66vd2mdu	2295	36	2	2	CD
cord-014597-66vd2mdu	2295	37	MSYS	MSYS	NNP
cord-014597-66vd2mdu	2295	38	,	,	,
cord-014597-66vd2mdu	2295	39	Helmholtz	Helmholtz	NNP
cord-014597-66vd2mdu	2295	40	Centre	Centre	NNP
cord-014597-66vd2mdu	2295	41	for	for	IN
cord-014597-66vd2mdu	2295	42	Infection	Infection	NNP
cord-014597-66vd2mdu	2295	43	Research	Research	NNP
cord-014597-66vd2mdu	2295	44	,	,	,
cord-014597-66vd2mdu	2295	45	Braunschweig	Braunschweig	NNP
cord-014597-66vd2mdu	2295	46	,	,	,
cord-014597-66vd2mdu	2295	47	Germany	Germany	NNP
cord-014597-66vd2mdu	2295	48	;	;	:
cord-014597-66vd2mdu	2295	49	3	3	CD
cord-014597-66vd2mdu	2295	50	Greiner	Greiner	NNP
cord-014597-66vd2mdu	2295	51	Bio	Bio	NNP
cord-014597-66vd2mdu	2295	52	-	-	HYPH
cord-014597-66vd2mdu	2295	53	One	one	CD
cord-014597-66vd2mdu	2295	54	GmbH	GmbH	NNP
cord-014597-66vd2mdu	2295	55	,	,	,
cord-014597-66vd2mdu	2295	56	Frickenhausen	Frickenhausen	NNP
cord-014597-66vd2mdu	2295	57	,	,	,
cord-014597-66vd2mdu	2295	58	Germany	Germany	NNP
cord-014597-66vd2mdu	2295	59	BMC	BMC	NNP
cord-014597-66vd2mdu	2295	60	Proceedings	Proceedings	NNP
cord-014597-66vd2mdu	2295	61	2018	2018	CD
cord-014597-66vd2mdu	2295	62	,	,	,
cord-014597-66vd2mdu	2296	1	12(Suppl	12(suppl	CD
cord-014597-66vd2mdu	2296	2	1):P-381	1):p-381	CD
cord-014597-66vd2mdu	2297	1	Background	background	VB
cord-014597-66vd2mdu	2297	2	A	a	DT
cord-014597-66vd2mdu	2297	3	major	major	JJ
cord-014597-66vd2mdu	2297	4	challenge	challenge	NN
cord-014597-66vd2mdu	2297	5	of	of	IN
cord-014597-66vd2mdu	2297	6	current	current	JJ
cord-014597-66vd2mdu	2297	7	research	research	NN
cord-014597-66vd2mdu	2297	8	is	be	VBZ
cord-014597-66vd2mdu	2297	9	the	the	DT
cord-014597-66vd2mdu	2297	10	limited	limited	JJ
cord-014597-66vd2mdu	2297	11	availability	availability	NN
cord-014597-66vd2mdu	2297	12	of	of	IN
cord-014597-66vd2mdu	2297	13	physiologically	physiologically	RB
cord-014597-66vd2mdu	2297	14	relevant	relevant	JJ
cord-014597-66vd2mdu	2297	15	cells	cell	NNS
cord-014597-66vd2mdu	2297	16	[	[	-LRB-
cord-014597-66vd2mdu	2297	17	1	1	CD
cord-014597-66vd2mdu	2297	18	]	]	-RRB-
cord-014597-66vd2mdu	2297	19	.	.	.
cord-014597-66vd2mdu	2298	1	Thus	thus	RB
cord-014597-66vd2mdu	2298	2	the	the	DT
cord-014597-66vd2mdu	2298	3	development	development	NN
cord-014597-66vd2mdu	2298	4	of	of	IN
cord-014597-66vd2mdu	2298	5	relevant	relevant	JJ
cord-014597-66vd2mdu	2298	6	cellular	cellular	JJ
cord-014597-66vd2mdu	2298	7	bioassays	bioassay	NNS
cord-014597-66vd2mdu	2298	8	that	that	WDT
cord-014597-66vd2mdu	2298	9	are	be	VBP
cord-014597-66vd2mdu	2298	10	robust	robust	JJ
cord-014597-66vd2mdu	2298	11	,	,	,
cord-014597-66vd2mdu	2298	12	reproducible	reproducible	JJ
cord-014597-66vd2mdu	2298	13	and	and	CC
cord-014597-66vd2mdu	2298	14	scalable	scalable	JJ
cord-014597-66vd2mdu	2298	15	is	be	VBZ
cord-014597-66vd2mdu	2298	16	hindered	hinder	VBN
cord-014597-66vd2mdu	2298	17	.	.	.
cord-014597-66vd2mdu	2299	1	To	to	TO
cord-014597-66vd2mdu	2299	2	overcome	overcome	VB
cord-014597-66vd2mdu	2299	3	current	current	JJ
cord-014597-66vd2mdu	2299	4	limitations	limitation	NNS
cord-014597-66vd2mdu	2299	5	we	-PRON-	PRP
cord-014597-66vd2mdu	2299	6	developed	develop	VBD
cord-014597-66vd2mdu	2299	7	an	an	DT
cord-014597-66vd2mdu	2299	8	immortalization	immortalization	NN
cord-014597-66vd2mdu	2299	9	strategy	strategy	NN
cord-014597-66vd2mdu	2299	10	allowing	allow	VBG
cord-014597-66vd2mdu	2299	11	the	the	DT
cord-014597-66vd2mdu	2299	12	efficient	efficient	JJ
cord-014597-66vd2mdu	2299	13	and	and	CC
cord-014597-66vd2mdu	2299	14	reproducible	reproducible	JJ
cord-014597-66vd2mdu	2299	15	establishment	establishment	NN
cord-014597-66vd2mdu	2299	16	of	of	IN
cord-014597-66vd2mdu	2299	17	novel	novel	JJ
cord-014597-66vd2mdu	2299	18	cell	cell	NN
cord-014597-66vd2mdu	2299	19	lines	line	NNS
cord-014597-66vd2mdu	2299	20	showing	show	VBG
cord-014597-66vd2mdu	2299	21	an	an	DT
cord-014597-66vd2mdu	2299	22	in	in	IN
cord-014597-66vd2mdu	2299	23	vivo	vivo	NN
cord-014597-66vd2mdu	2299	24	-	-	HYPH
cord-014597-66vd2mdu	2299	25	like	like	JJ
cord-014597-66vd2mdu	2299	26	phenotype	phenotype	NN
cord-014597-66vd2mdu	2299	27	.	.	.
cord-014597-66vd2mdu	2300	1	The	the	DT
cord-014597-66vd2mdu	2300	2	main	main	JJ
cord-014597-66vd2mdu	2300	3	feature	feature	NN
cord-014597-66vd2mdu	2300	4	of	of	IN
cord-014597-66vd2mdu	2300	5	our	-PRON-	PRP$
cord-014597-66vd2mdu	2300	6	CI	ci	NN
cord-014597-66vd2mdu	2300	7	-	-	HYPH
cord-014597-66vd2mdu	2300	8	SCREEN	screen	NN
cord-014597-66vd2mdu	2300	9	technology	technology	NN
cord-014597-66vd2mdu	2300	10	is	be	VBZ
cord-014597-66vd2mdu	2300	11	the	the	DT
cord-014597-66vd2mdu	2300	12	ability	ability	NN
cord-014597-66vd2mdu	2300	13	to	to	TO
cord-014597-66vd2mdu	2300	14	combine	combine	VB
cord-014597-66vd2mdu	2300	15	the	the	DT
cord-014597-66vd2mdu	2300	16	advantage	advantage	NN
cord-014597-66vd2mdu	2300	17	of	of	IN
cord-014597-66vd2mdu	2300	18	cell	cell	NN
cord-014597-66vd2mdu	2300	19	linesthe	linesthe	DT
cord-014597-66vd2mdu	2300	20	unlimited	unlimited	JJ
cord-014597-66vd2mdu	2300	21	cell	cell	NN
cord-014597-66vd2mdu	2300	22	supplywith	supplywith	NN
cord-014597-66vd2mdu	2300	23	the	the	DT
cord-014597-66vd2mdu	2300	24	advantage	advantage	NN
cord-014597-66vd2mdu	2300	25	of	of	IN
cord-014597-66vd2mdu	2300	26	primary	primary	JJ
cord-014597-66vd2mdu	2300	27	cellsthe	cellsthe	DT
cord-014597-66vd2mdu	2300	28	physiological	physiological	JJ
cord-014597-66vd2mdu	2300	29	relevance	relevance	NN
cord-014597-66vd2mdu	2300	30	.	.	.
cord-014597-66vd2mdu	2301	1	Using	use	VBG
cord-014597-66vd2mdu	2301	2	this	this	DT
cord-014597-66vd2mdu	2301	3	technology	technology	NN
cord-014597-66vd2mdu	2301	4	we	-PRON-	PRP
cord-014597-66vd2mdu	2301	5	have	have	VBP
cord-014597-66vd2mdu	2301	6	immortalized	immortalize	VBN
cord-014597-66vd2mdu	2301	7	,	,	,
cord-014597-66vd2mdu	2301	8	amongst	amongst	IN
cord-014597-66vd2mdu	2301	9	others	other	NNS
cord-014597-66vd2mdu	2301	10	,	,	,
cord-014597-66vd2mdu	2301	11	a	a	DT
cord-014597-66vd2mdu	2301	12	human	human	JJ
cord-014597-66vd2mdu	2301	13	osteoblast	osteoblast	JJ
cord-014597-66vd2mdu	2301	14	cell	cell	NN
cord-014597-66vd2mdu	2301	15	line	line	NN
cord-014597-66vd2mdu	2301	16	(	(	-LRB-
cord-014597-66vd2mdu	2301	17	CI	CI	NNP
cord-014597-66vd2mdu	2301	18	-	-	HYPH
cord-014597-66vd2mdu	2301	19	huOB	huOB	NNP
cord-014597-66vd2mdu	2301	20	)	)	-RRB-
cord-014597-66vd2mdu	2301	21	[	[	-LRB-
cord-014597-66vd2mdu	2301	22	2	2	CD
cord-014597-66vd2mdu	2301	23	]	]	-RRB-
cord-014597-66vd2mdu	2301	24	.	.	.
cord-014597-66vd2mdu	2302	1	In	in	IN
cord-014597-66vd2mdu	2302	2	the	the	DT
cord-014597-66vd2mdu	2302	3	present	present	JJ
cord-014597-66vd2mdu	2302	4	study	study	NN
cord-014597-66vd2mdu	2302	5	,	,	,
cord-014597-66vd2mdu	2302	6	the	the	DT
cord-014597-66vd2mdu	2302	7	in	in	IN
cord-014597-66vd2mdu	2302	8	vivo	vivo	NN
cord-014597-66vd2mdu	2302	9	-	-	HYPH
cord-014597-66vd2mdu	2302	10	like	like	JJ
cord-014597-66vd2mdu	2302	11	phenotype	phenotype	NN
cord-014597-66vd2mdu	2302	12	and	and	CC
cord-014597-66vd2mdu	2302	13	functionality	functionality	NN
cord-014597-66vd2mdu	2302	14	of	of	IN
cord-014597-66vd2mdu	2302	15	the	the	DT
cord-014597-66vd2mdu	2302	16	novel	novel	JJ
cord-014597-66vd2mdu	2302	17	CI	CI	NNP
cord-014597-66vd2mdu	2302	18	-	-	HYPH
cord-014597-66vd2mdu	2302	19	huOB	huOB	NNP
cord-014597-66vd2mdu	2302	20	was	be	VBD
cord-014597-66vd2mdu	2302	21	examined	examine	VBN
cord-014597-66vd2mdu	2302	22	.	.	.
cord-014597-66vd2mdu	2303	1	Therefore	therefore	RB
cord-014597-66vd2mdu	2303	2	,	,	,
cord-014597-66vd2mdu	2303	3	CI	CI	NNP
cord-014597-66vd2mdu	2303	4	-	-	HYPH
cord-014597-66vd2mdu	2303	5	huOB	huOB	NNP
cord-014597-66vd2mdu	2303	6	cells	cell	NNS
cord-014597-66vd2mdu	2303	7	were	be	VBD
cord-014597-66vd2mdu	2303	8	used	use	VBN
cord-014597-66vd2mdu	2303	9	to	to	TO
cord-014597-66vd2mdu	2303	10	develop	develop	VB
cord-014597-66vd2mdu	2303	11	a	a	DT
cord-014597-66vd2mdu	2303	12	3D	3d	JJ
cord-014597-66vd2mdu	2303	13	cell	cell	NN
cord-014597-66vd2mdu	2303	14	culture	culture	NN
cord-014597-66vd2mdu	2303	15	model	model	NN
cord-014597-66vd2mdu	2303	16	by	by	IN
cord-014597-66vd2mdu	2303	17	using	use	VBG
cord-014597-66vd2mdu	2303	18	the	the	DT
cord-014597-66vd2mdu	2303	19	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2303	20	3D	3D	NNP
cord-014597-66vd2mdu	2303	21	bioprinting	bioprinting	NNP
cord-014597-66vd2mdu	2303	22	technology	technology	NNP
cord-014597-66vd2mdu	2303	23	(	(	-LRB-
cord-014597-66vd2mdu	2303	24	Nano3D	Nano3D	NNP
cord-014597-66vd2mdu	2303	25	Biosciences	Biosciences	NNP
cord-014597-66vd2mdu	2303	26	,	,	,
cord-014597-66vd2mdu	2303	27	Houston	Houston	NNP
cord-014597-66vd2mdu	2303	28	,	,	,
cord-014597-66vd2mdu	2303	29	TX	TX	NNP
cord-014597-66vd2mdu	2303	30	,	,	,
cord-014597-66vd2mdu	2303	31	USA	USA	NNP
cord-014597-66vd2mdu	2303	32	)	)	-RRB-
cord-014597-66vd2mdu	2303	33	[	[	-LRB-
cord-014597-66vd2mdu	2303	34	3	3	CD
cord-014597-66vd2mdu	2303	35	]	]	-RRB-
cord-014597-66vd2mdu	2303	36	.	.	.
cord-014597-66vd2mdu	2304	1	The	the	DT
cord-014597-66vd2mdu	2304	2	CI	CI	NNP
cord-014597-66vd2mdu	2304	3	-	-	HYPH
cord-014597-66vd2mdu	2304	4	huOB	huOB	NNP
cord-014597-66vd2mdu	2304	5	cell	cell	NN
cord-014597-66vd2mdu	2304	6	line	line	NN
cord-014597-66vd2mdu	2304	7	was	be	VBD
cord-014597-66vd2mdu	2304	8	recently	recently	RB
cord-014597-66vd2mdu	2304	9	described	describe	VBN
cord-014597-66vd2mdu	2304	10	and	and	CC
cord-014597-66vd2mdu	2304	11	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2304	12	in	in	IN
cord-014597-66vd2mdu	2304	13	huOB	huOB	NNP
cord-014597-66vd2mdu	2304	14	Maintenance	Maintenance	NNP
cord-014597-66vd2mdu	2304	15	Medium	medium	NN
cord-014597-66vd2mdu	2304	16	(	(	-LRB-
cord-014597-66vd2mdu	2304	17	InSCREENeX	inscreenex	NN
cord-014597-66vd2mdu	2304	18	,	,	,
cord-014597-66vd2mdu	2304	19	Germany	Germany	NNP
cord-014597-66vd2mdu	2304	20	)	)	-RRB-
cord-014597-66vd2mdu	2304	21	.	.	.
cord-014597-66vd2mdu	2305	1	For	for	IN
cord-014597-66vd2mdu	2305	2	spheroid	spheroid	NN
cord-014597-66vd2mdu	2305	3	creation	creation	NN
cord-014597-66vd2mdu	2305	4	CI	CI	NNP
cord-014597-66vd2mdu	2305	5	-	-	HYPH
cord-014597-66vd2mdu	2305	6	huOBs	huOBs	NNP
cord-014597-66vd2mdu	2305	7	were	be	VBD
cord-014597-66vd2mdu	2305	8	grown	grow	VBN
cord-014597-66vd2mdu	2305	9	in	in	IN
cord-014597-66vd2mdu	2305	10	a	a	DT
cord-014597-66vd2mdu	2305	11	monolayer	monolayer	NN
cord-014597-66vd2mdu	2305	12	,	,	,
cord-014597-66vd2mdu	2305	13	magnetized	magnetize	VBN
cord-014597-66vd2mdu	2305	14	by	by	IN
cord-014597-66vd2mdu	2305	15	adding	add	VBG
cord-014597-66vd2mdu	2305	16	a	a	DT
cord-014597-66vd2mdu	2305	17	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2305	18	nanoparticle	nanoparticle	NN
cord-014597-66vd2mdu	2305	19	assembly	assembly	NN
cord-014597-66vd2mdu	2305	20	(	(	-LRB-
cord-014597-66vd2mdu	2305	21	NanoShuttle	NanoShuttle	NNP
cord-014597-66vd2mdu	2305	22	,	,	,
cord-014597-66vd2mdu	2305	23	NS	NS	NNP
cord-014597-66vd2mdu	2305	24	,	,	,
cord-014597-66vd2mdu	2305	25	Nano3D	Nano3D	NNP
cord-014597-66vd2mdu	2305	26	Biosciences	Biosciences	NNP
cord-014597-66vd2mdu	2305	27	,	,	,
cord-014597-66vd2mdu	2305	28	Houston	Houston	NNP
cord-014597-66vd2mdu	2305	29	,	,	,
cord-014597-66vd2mdu	2305	30	TX	TX	NNP
cord-014597-66vd2mdu	2305	31	,	,	,
cord-014597-66vd2mdu	2305	32	USA	USA	NNP
cord-014597-66vd2mdu	2305	33	)	)	-RRB-
cord-014597-66vd2mdu	2305	34	at	at	IN
cord-014597-66vd2mdu	2305	35	a	a	DT
cord-014597-66vd2mdu	2305	36	concentration	concentration	NN
cord-014597-66vd2mdu	2305	37	of	of	IN
cord-014597-66vd2mdu	2305	38	4μl	4μl	JJ
cord-014597-66vd2mdu	2305	39	NS	NS	NNP
cord-014597-66vd2mdu	2305	40	/	/	SYM
cord-014597-66vd2mdu	2305	41	cm	cm	NNP
cord-014597-66vd2mdu	2305	42	2	2	CD
cord-014597-66vd2mdu	2305	43	growth	growth	NN
cord-014597-66vd2mdu	2305	44	area	area	NN
cord-014597-66vd2mdu	2305	45	.	.	.
cord-014597-66vd2mdu	2306	1	After	after	IN
cord-014597-66vd2mdu	2306	2	an	an	DT
cord-014597-66vd2mdu	2306	3	overnight	overnight	JJ
cord-014597-66vd2mdu	2306	4	incubation	incubation	NN
cord-014597-66vd2mdu	2306	5	magnetized	magnetize	VBD
cord-014597-66vd2mdu	2306	6	CI	CI	NNP
cord-014597-66vd2mdu	2306	7	-	-	HYPH
cord-014597-66vd2mdu	2306	8	huOB	huOB	NNP
cord-014597-66vd2mdu	2306	9	were	be	VBD
cord-014597-66vd2mdu	2306	10	detached	detach	VBN
cord-014597-66vd2mdu	2306	11	and	and	CC
cord-014597-66vd2mdu	2306	12	seeded	seed	VBN
cord-014597-66vd2mdu	2306	13	into	into	IN
cord-014597-66vd2mdu	2306	14	Cellstar	Cellstar	NNP
cord-014597-66vd2mdu	2306	15	®	®	.
cord-014597-66vd2mdu	2306	16	Cell	cell	NN
cord-014597-66vd2mdu	2306	17	-	-	HYPH
cord-014597-66vd2mdu	2306	18	Repellent	Repellent	NNP
cord-014597-66vd2mdu	2306	19	96-well	96-well	CD
cord-014597-66vd2mdu	2306	20	plates	plate	NNS
cord-014597-66vd2mdu	2307	1	(	(	-LRB-
cord-014597-66vd2mdu	2307	2	Greiner	Greiner	NNP
cord-014597-66vd2mdu	2307	3	Bio	Bio	NNP
cord-014597-66vd2mdu	2307	4	-	-	HYPH
cord-014597-66vd2mdu	2307	5	One	One	NNP
cord-014597-66vd2mdu	2307	6	,	,	,
cord-014597-66vd2mdu	2307	7	Frickenhausen	Frickenhausen	NNP
cord-014597-66vd2mdu	2307	8	,	,	,
cord-014597-66vd2mdu	2307	9	Germany	Germany	NNP
cord-014597-66vd2mdu	2307	10	)	)	-RRB-
cord-014597-66vd2mdu	2307	11	.	.	.
cord-014597-66vd2mdu	2308	1	With	with	IN
cord-014597-66vd2mdu	2308	2	the	the	DT
cord-014597-66vd2mdu	2308	3	help	help	NN
cord-014597-66vd2mdu	2308	4	of	of	IN
cord-014597-66vd2mdu	2308	5	mild	mild	JJ
cord-014597-66vd2mdu	2308	6	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2308	7	forces	force	NNS
cord-014597-66vd2mdu	2308	8	cells	cell	NNS
cord-014597-66vd2mdu	2308	9	were	be	VBD
cord-014597-66vd2mdu	2308	10	printed	print	VBN
cord-014597-66vd2mdu	2308	11	into	into	IN
cord-014597-66vd2mdu	2308	12	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2308	13	within	within	IN
cord-014597-66vd2mdu	2308	14	2h	2h	CD
cord-014597-66vd2mdu	2308	15	.	.	.
cord-014597-66vd2mdu	2309	1	These	these	DT
cord-014597-66vd2mdu	2309	2	consist	consist	VBP
cord-014597-66vd2mdu	2309	3	of	of	IN
cord-014597-66vd2mdu	2309	4	1.000	1.000	CD
cord-014597-66vd2mdu	2309	5	-	-	SYM
cord-014597-66vd2mdu	2309	6	50.000	50.000	CD
cord-014597-66vd2mdu	2309	7	cells	cell	NNS
cord-014597-66vd2mdu	2309	8	and	and	CC
cord-014597-66vd2mdu	2309	9	were	be	VBD
cord-014597-66vd2mdu	2309	10	cultured	culture	VBN
cord-014597-66vd2mdu	2309	11	for	for	IN
cord-014597-66vd2mdu	2309	12	a	a	DT
cord-014597-66vd2mdu	2309	13	period	period	NN
cord-014597-66vd2mdu	2309	14	of	of	IN
cord-014597-66vd2mdu	2309	15	up	up	IN
cord-014597-66vd2mdu	2309	16	to	to	TO
cord-014597-66vd2mdu	2309	17	50	50	CD
cord-014597-66vd2mdu	2309	18	days	day	NNS
cord-014597-66vd2mdu	2309	19	.	.	.
cord-014597-66vd2mdu	2310	1	The	the	DT
cord-014597-66vd2mdu	2310	2	cell	cell	NN
cord-014597-66vd2mdu	2310	3	viability	viability	NN
cord-014597-66vd2mdu	2310	4	was	be	VBD
cord-014597-66vd2mdu	2310	5	analyzed	analyze	VBN
cord-014597-66vd2mdu	2310	6	by	by	IN
cord-014597-66vd2mdu	2310	7	a	a	DT
cord-014597-66vd2mdu	2310	8	Propidium	Propidium	NNP
cord-014597-66vd2mdu	2310	9	iodide	iodide	NN
cord-014597-66vd2mdu	2310	10	(	(	-LRB-
cord-014597-66vd2mdu	2310	11	PI	PI	NNP
cord-014597-66vd2mdu	2310	12	)	)	-RRB-
cord-014597-66vd2mdu	2310	13	and	and	CC
cord-014597-66vd2mdu	2310	14	Calcein	Calcein	NNP
cord-014597-66vd2mdu	2310	15	AM	am	VBP
cord-014597-66vd2mdu	2310	16	staining	stain	VBG
cord-014597-66vd2mdu	2310	17	.	.	.
cord-014597-66vd2mdu	2311	1	To	to	TO
cord-014597-66vd2mdu	2311	2	improve	improve	VB
cord-014597-66vd2mdu	2311	3	spheroid	spheroid	NN
cord-014597-66vd2mdu	2311	4	functionality	functionality	NN
cord-014597-66vd2mdu	2311	5	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2311	6	were	be	VBD
cord-014597-66vd2mdu	2311	7	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2311	8	with	with	IN
cord-014597-66vd2mdu	2311	9	huOB	huOB	NNP
cord-014597-66vd2mdu	2311	10	Differentiation	Differentiation	NNP
cord-014597-66vd2mdu	2311	11	Medium	Medium	NNP
cord-014597-66vd2mdu	2311	12	(	(	-LRB-
cord-014597-66vd2mdu	2311	13	InSCREENeX	inscreenex	NN
cord-014597-66vd2mdu	2311	14	,	,	,
cord-014597-66vd2mdu	2311	15	Germany	Germany	NNP
cord-014597-66vd2mdu	2311	16	)	)	-RRB-
cord-014597-66vd2mdu	2311	17	.	.	.
cord-014597-66vd2mdu	2312	1	"	"	``
cord-014597-66vd2mdu	2312	2	Mini	mini	JJ
cord-014597-66vd2mdu	2312	3	bone	bone	NN
cord-014597-66vd2mdu	2312	4	"	"	''
cord-014597-66vd2mdu	2312	5	tissue	tissue	NN
cord-014597-66vd2mdu	2312	6	functionality	functionality	NN
cord-014597-66vd2mdu	2312	7	and	and	CC
cord-014597-66vd2mdu	2312	8	thus	thus	RB
cord-014597-66vd2mdu	2312	9	mineralization	mineralization	NN
cord-014597-66vd2mdu	2312	10	was	be	VBD
cord-014597-66vd2mdu	2312	11	analyzed	analyze	VBN
cord-014597-66vd2mdu	2312	12	by	by	IN
cord-014597-66vd2mdu	2312	13	an	an	DT
cord-014597-66vd2mdu	2312	14	alkaline	alkaline	JJ
cord-014597-66vd2mdu	2312	15	phosphatase	phosphatase	NN
cord-014597-66vd2mdu	2312	16	(	(	-LRB-
cord-014597-66vd2mdu	2312	17	alkaline	alkaline	JJ
cord-014597-66vd2mdu	2312	18	phosphatase	phosphatase	NN
cord-014597-66vd2mdu	2312	19	activity	activity	NN
cord-014597-66vd2mdu	2312	20	)	)	-RRB-
cord-014597-66vd2mdu	2312	21	and	and	CC
cord-014597-66vd2mdu	2312	22	an	an	DT
cord-014597-66vd2mdu	2312	23	alizarin	alizarin	JJ
cord-014597-66vd2mdu	2312	24	red	red	JJ
cord-014597-66vd2mdu	2312	25	S	s	NN
cord-014597-66vd2mdu	2312	26	Staining	Staining	NNP
cord-014597-66vd2mdu	2312	27	(	(	-LRB-
cord-014597-66vd2mdu	2312	28	Ca	Ca	NNP
cord-014597-66vd2mdu	2312	29	2	2	CD
cord-014597-66vd2mdu	2312	30	+	+	SYM
cord-014597-66vd2mdu	2312	31	deposits	deposit	NNS
cord-014597-66vd2mdu	2312	32	)	)	-RRB-
cord-014597-66vd2mdu	2312	33	.	.	.
cord-014597-66vd2mdu	2313	1	The	the	DT
cord-014597-66vd2mdu	2313	2	combination	combination	NN
cord-014597-66vd2mdu	2313	3	of	of	IN
cord-014597-66vd2mdu	2313	4	CI	CI	NNP
cord-014597-66vd2mdu	2313	5	-	-	HYPH
cord-014597-66vd2mdu	2313	6	huOB	huOB	NNP
cord-014597-66vd2mdu	2313	7	cells	cell	NNS
cord-014597-66vd2mdu	2313	8	with	with	IN
cord-014597-66vd2mdu	2313	9	the	the	DT
cord-014597-66vd2mdu	2313	10	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2313	11	3D	3D	NNP
cord-014597-66vd2mdu	2313	12	bioprinting	bioprinting	NNP
cord-014597-66vd2mdu	2313	13	technology	technology	NN
cord-014597-66vd2mdu	2313	14	enabled	enable	VBD
cord-014597-66vd2mdu	2313	15	the	the	DT
cord-014597-66vd2mdu	2313	16	establishment	establishment	NN
cord-014597-66vd2mdu	2313	17	of	of	IN
cord-014597-66vd2mdu	2313	18	reproducible	reproducible	JJ
cord-014597-66vd2mdu	2313	19	and	and	CC
cord-014597-66vd2mdu	2313	20	consistent	consistent	JJ
cord-014597-66vd2mdu	2313	21	3D	3d	JJ
cord-014597-66vd2mdu	2313	22	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2313	23	.	.	.
cord-014597-66vd2mdu	2314	1	Single	single	JJ
cord-014597-66vd2mdu	2314	2	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2314	3	per	per	IN
cord-014597-66vd2mdu	2314	4	well	well	RB
cord-014597-66vd2mdu	2314	5	were	be	VBD
cord-014597-66vd2mdu	2314	6	formed	form	VBN
cord-014597-66vd2mdu	2314	7	independent	independent	JJ
cord-014597-66vd2mdu	2314	8	of	of	IN
cord-014597-66vd2mdu	2314	9	the	the	DT
cord-014597-66vd2mdu	2314	10	amount	amount	NN
cord-014597-66vd2mdu	2314	11	of	of	IN
cord-014597-66vd2mdu	2314	12	cells	cell	NNS
cord-014597-66vd2mdu	2314	13	(	(	-LRB-
cord-014597-66vd2mdu	2314	14	1.000	1.000	CD
cord-014597-66vd2mdu	2314	15	-	-	SYM
cord-014597-66vd2mdu	2314	16	50.000	50.000	CD
cord-014597-66vd2mdu	2314	17	cells	cell	NNS
cord-014597-66vd2mdu	2314	18	)	)	-RRB-
cord-014597-66vd2mdu	2315	1	(	(	-LRB-
cord-014597-66vd2mdu	2315	2	Fig	Fig	NNP
cord-014597-66vd2mdu	2315	3	.	.	.
cord-014597-66vd2mdu	2315	4	1a	1a	CD
cord-014597-66vd2mdu	2315	5	)	)	-RRB-
cord-014597-66vd2mdu	2315	6	.	.	.
cord-014597-66vd2mdu	2316	1	Formed	form	VBN
cord-014597-66vd2mdu	2316	2	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2316	3	were	be	VBD
cord-014597-66vd2mdu	2316	4	stable	stable	JJ
cord-014597-66vd2mdu	2316	5	for	for	IN
cord-014597-66vd2mdu	2316	6	a	a	DT
cord-014597-66vd2mdu	2316	7	culture	culture	NN
cord-014597-66vd2mdu	2316	8	period	period	NN
cord-014597-66vd2mdu	2316	9	of	of	IN
cord-014597-66vd2mdu	2316	10	up	up	IN
cord-014597-66vd2mdu	2316	11	to	to	TO
cord-014597-66vd2mdu	2316	12	50	50	CD
cord-014597-66vd2mdu	2316	13	days	day	NNS
cord-014597-66vd2mdu	2316	14	(	(	-LRB-
cord-014597-66vd2mdu	2316	15	Fig	fig	NN
cord-014597-66vd2mdu	2316	16	.	.	.
cord-014597-66vd2mdu	2316	17	1b	1b	CD
cord-014597-66vd2mdu	2316	18	)	)	-RRB-
cord-014597-66vd2mdu	2316	19	.	.	.
cord-014597-66vd2mdu	2317	1	Neither	neither	DT
cord-014597-66vd2mdu	2317	2	cell	cell	NN
cord-014597-66vd2mdu	2317	3	death	death	NN
cord-014597-66vd2mdu	2317	4	nor	nor	CC
cord-014597-66vd2mdu	2317	5	cell	cell	NN
cord-014597-66vd2mdu	2317	6	proliferation	proliferation	NN
cord-014597-66vd2mdu	2317	7	were	be	VBD
cord-014597-66vd2mdu	2317	8	observed	observe	VBN
cord-014597-66vd2mdu	2317	9	in	in	IN
cord-014597-66vd2mdu	2317	10	the	the	DT
cord-014597-66vd2mdu	2317	11	bioprinted	bioprinte	VBN
cord-014597-66vd2mdu	2317	12	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2317	13	which	which	WDT
cord-014597-66vd2mdu	2317	14	is	be	VBZ
cord-014597-66vd2mdu	2317	15	indicated	indicate	VBN
cord-014597-66vd2mdu	2317	16	by	by	IN
cord-014597-66vd2mdu	2317	17	the	the	DT
cord-014597-66vd2mdu	2317	18	stable	stable	JJ
cord-014597-66vd2mdu	2317	19	size	size	NN
cord-014597-66vd2mdu	2317	20	of	of	IN
cord-014597-66vd2mdu	2317	21	the	the	DT
cord-014597-66vd2mdu	2317	22	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2317	23	throughout	throughout	IN
cord-014597-66vd2mdu	2317	24	the	the	DT
cord-014597-66vd2mdu	2317	25	cultivation	cultivation	NN
cord-014597-66vd2mdu	2317	26	(	(	-LRB-
cord-014597-66vd2mdu	2317	27	Fig	Fig	NNP
cord-014597-66vd2mdu	2317	28	.	.	.
cord-014597-66vd2mdu	2317	29	1c	1c	CD
cord-014597-66vd2mdu	2317	30	)	)	-RRB-
cord-014597-66vd2mdu	2317	31	.	.	.
cord-014597-66vd2mdu	2318	1	After	after	IN
cord-014597-66vd2mdu	2318	2	treatment	treatment	NN
cord-014597-66vd2mdu	2318	3	with	with	IN
cord-014597-66vd2mdu	2318	4	a	a	DT
cord-014597-66vd2mdu	2318	5	differentiation	differentiation	NN
cord-014597-66vd2mdu	2318	6	stimulus	stimulus	NN
cord-014597-66vd2mdu	2318	7	the	the	DT
cord-014597-66vd2mdu	2318	8	3D	3D	NNP
cord-014597-66vd2mdu	2318	9	bioprinted	bioprinted	NNP
cord-014597-66vd2mdu	2318	10	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2318	11	became	become	VBD
cord-014597-66vd2mdu	2318	12	fully	fully	RB
cord-014597-66vd2mdu	2318	13	functional	functional	JJ
cord-014597-66vd2mdu	2318	14	"	"	''
cord-014597-66vd2mdu	2318	15	mini	mini	JJ
cord-014597-66vd2mdu	2318	16	bones	bone	NNS
cord-014597-66vd2mdu	2318	17	"	"	''
cord-014597-66vd2mdu	2318	18	.	.	.
cord-014597-66vd2mdu	2319	1	This	this	DT
cord-014597-66vd2mdu	2319	2	was	be	VBD
cord-014597-66vd2mdu	2319	3	highlighted	highlight	VBN
cord-014597-66vd2mdu	2319	4	by	by	IN
cord-014597-66vd2mdu	2319	5	the	the	DT
cord-014597-66vd2mdu	2319	6	alkaline	alkaline	JJ
cord-014597-66vd2mdu	2319	7	phosphatase	phosphatase	NN
cord-014597-66vd2mdu	2319	8	activity	activity	NN
cord-014597-66vd2mdu	2319	9	and	and	CC
cord-014597-66vd2mdu	2319	10	the	the	DT
cord-014597-66vd2mdu	2319	11	Ca	Ca	NNP
cord-014597-66vd2mdu	2319	12	2	2	CD
cord-014597-66vd2mdu	2319	13	+	+	SYM
cord-014597-66vd2mdu	2319	14	deposits	deposit	NNS
cord-014597-66vd2mdu	2319	15	within	within	IN
cord-014597-66vd2mdu	2319	16	the	the	DT
cord-014597-66vd2mdu	2319	17	3D	3D	NNP
cord-014597-66vd2mdu	2319	18	bioprinted	bioprinte	VBN
cord-014597-66vd2mdu	2319	19	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2319	20	(	(	-LRB-
cord-014597-66vd2mdu	2319	21	Fig	Fig	NNP
cord-014597-66vd2mdu	2319	22	.	.	NNP
cord-014597-66vd2mdu	2319	23	1d	1d	NNP
cord-014597-66vd2mdu	2319	24	,	,	,
cord-014597-66vd2mdu	2319	25	e	e	LS
cord-014597-66vd2mdu	2319	26	)	)	-RRB-
cord-014597-66vd2mdu	2319	27	.	.	.
cord-014597-66vd2mdu	2320	1	Taken	take	VBN
cord-014597-66vd2mdu	2320	2	together	together	RB
cord-014597-66vd2mdu	2320	3	,	,	,
cord-014597-66vd2mdu	2320	4	these	these	DT
cord-014597-66vd2mdu	2320	5	results	result	NNS
cord-014597-66vd2mdu	2320	6	demonstrated	demonstrate	VBD
cord-014597-66vd2mdu	2320	7	that	that	IN
cord-014597-66vd2mdu	2320	8	the	the	DT
cord-014597-66vd2mdu	2320	9	functional	functional	JJ
cord-014597-66vd2mdu	2320	10	immortalization	immortalization	NN
cord-014597-66vd2mdu	2320	11	technology	technology	NN
cord-014597-66vd2mdu	2320	12	provides	provide	VBZ
cord-014597-66vd2mdu	2320	13	physiologically	physiologically	RB
cord-014597-66vd2mdu	2320	14	relevant	relevant	JJ
cord-014597-66vd2mdu	2320	15	cells	cell	NNS
cord-014597-66vd2mdu	2320	16	in	in	IN
cord-014597-66vd2mdu	2320	17	sufficient	sufficient	JJ
cord-014597-66vd2mdu	2320	18	numbers	number	NNS
cord-014597-66vd2mdu	2320	19	and	and	CC
cord-014597-66vd2mdu	2320	20	that	that	IN
cord-014597-66vd2mdu	2320	21	the	the	DT
cord-014597-66vd2mdu	2320	22	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2320	23	3D	3D	NNP
cord-014597-66vd2mdu	2320	24	Bioprinting	Bioprinting	NNP
cord-014597-66vd2mdu	2320	25	technology	technology	NN
cord-014597-66vd2mdu	2320	26	enabled	enable	VBD
cord-014597-66vd2mdu	2320	27	a	a	DT
cord-014597-66vd2mdu	2320	28	fast	fast	JJ
cord-014597-66vd2mdu	2320	29	,	,	,
cord-014597-66vd2mdu	2320	30	consistent	consistent	JJ
cord-014597-66vd2mdu	2320	31	cell	cell	NN
cord-014597-66vd2mdu	2320	32	aggregation	aggregation	NN
cord-014597-66vd2mdu	2320	33	and	and	CC
cord-014597-66vd2mdu	2320	34	the	the	DT
cord-014597-66vd2mdu	2320	35	formation	formation	NN
cord-014597-66vd2mdu	2320	36	of	of	IN
cord-014597-66vd2mdu	2320	37	stable	stable	JJ
cord-014597-66vd2mdu	2320	38	uniform	uniform	JJ
cord-014597-66vd2mdu	2320	39	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2320	40	.	.	.
cord-014597-66vd2mdu	2321	1	Importantly	importantly	RB
cord-014597-66vd2mdu	2321	2	,	,	,
cord-014597-66vd2mdu	2321	3	these	these	DT
cord-014597-66vd2mdu	2321	4	immortalized	immortalize	VBN
cord-014597-66vd2mdu	2321	5	cells	cell	NNS
cord-014597-66vd2mdu	2321	6	are	be	VBP
cord-014597-66vd2mdu	2321	7	capable	capable	JJ
cord-014597-66vd2mdu	2321	8	to	to	TO
cord-014597-66vd2mdu	2321	9	differentiate	differentiate	VB
cord-014597-66vd2mdu	2321	10	when	when	WRB
cord-014597-66vd2mdu	2321	11	a	a	DT
cord-014597-66vd2mdu	2321	12	suitable	suitable	JJ
cord-014597-66vd2mdu	2321	13	stimulus	stimulus	NN
cord-014597-66vd2mdu	2321	14	is	be	VBZ
cord-014597-66vd2mdu	2321	15	provided	provide	VBN
cord-014597-66vd2mdu	2321	16	.	.	.
cord-014597-66vd2mdu	2322	1	For	for	IN
cord-014597-66vd2mdu	2322	2	differentiation	differentiation	NN
cord-014597-66vd2mdu	2322	3	into	into	IN
cord-014597-66vd2mdu	2322	4	mini	mini	JJ
cord-014597-66vd2mdu	2322	5	bones	bone	NNS
cord-014597-66vd2mdu	2322	6	,	,	,
cord-014597-66vd2mdu	2322	7	3D	3d	JJ
cord-014597-66vd2mdu	2322	8	spheroid	spheroid	NN
cord-014597-66vd2mdu	2322	9	cultivation	cultivation	NN
cord-014597-66vd2mdu	2322	10	and	and	CC
cord-014597-66vd2mdu	2322	11	additional	additional	JJ
cord-014597-66vd2mdu	2322	12	stimulation	stimulation	NN
cord-014597-66vd2mdu	2322	13	by	by	IN
cord-014597-66vd2mdu	2322	14	small	small	JJ
cord-014597-66vd2mdu	2322	15	molecules	molecule	NNS
cord-014597-66vd2mdu	2322	16	are	be	VBP
cord-014597-66vd2mdu	2322	17	required	require	VBN
cord-014597-66vd2mdu	2322	18	.	.	.
cord-014597-66vd2mdu	2323	1	The	the	DT
cord-014597-66vd2mdu	2323	2	combination	combination	NN
cord-014597-66vd2mdu	2323	3	of	of	IN
cord-014597-66vd2mdu	2323	4	physiologically	physiologically	RB
cord-014597-66vd2mdu	2323	5	relevant	relevant	JJ
cord-014597-66vd2mdu	2323	6	cell	cell	NN
cord-014597-66vd2mdu	2323	7	systems	system	NNS
cord-014597-66vd2mdu	2323	8	with	with	IN
cord-014597-66vd2mdu	2323	9	three	three	CD
cord-014597-66vd2mdu	2323	10	dimensional	dimensional	JJ
cord-014597-66vd2mdu	2323	11	culturing	culturing	NN
cord-014597-66vd2mdu	2323	12	will	will	MD
cord-014597-66vd2mdu	2323	13	help	help	VB
cord-014597-66vd2mdu	2323	14	to	to	TO
cord-014597-66vd2mdu	2323	15	generate	generate	VB
cord-014597-66vd2mdu	2323	16	in	in	IN
cord-014597-66vd2mdu	2323	17	vitro	vitro	FW
cord-014597-66vd2mdu	2323	18	test	test	NN
cord-014597-66vd2mdu	2323	19	systems	system	NNS
cord-014597-66vd2mdu	2323	20	which	which	WDT
cord-014597-66vd2mdu	2323	21	closely	closely	RB
cord-014597-66vd2mdu	2323	22	resemble	resemble	VBP
cord-014597-66vd2mdu	2323	23	the	the	DT
cord-014597-66vd2mdu	2323	24	in	in	IN
cord-014597-66vd2mdu	2323	25	vivo	vivo	NN
cord-014597-66vd2mdu	2323	26	physiology	physiology	NN
cord-014597-66vd2mdu	2323	27	and	and	CC
cord-014597-66vd2mdu	2323	28	thereby	thereby	RB
cord-014597-66vd2mdu	2323	29	supporting	support	VBG
cord-014597-66vd2mdu	2323	30	future	future	JJ
cord-014597-66vd2mdu	2323	31	drug	drug	NN
cord-014597-66vd2mdu	2323	32	discovery	discovery	NN
cord-014597-66vd2mdu	2323	33	approaches	approach	NNS
cord-014597-66vd2mdu	2323	34	.	.	.
cord-014597-66vd2mdu	2324	1	Fig	Fig	NNP
cord-014597-66vd2mdu	2324	2	.	.	.
cord-014597-66vd2mdu	2324	3	1	1	CD
cord-014597-66vd2mdu	2324	4	(	(	-LRB-
cord-014597-66vd2mdu	2324	5	abstract	abstract	NNP
cord-014597-66vd2mdu	2324	6	P-381	P-381	NNP
cord-014597-66vd2mdu	2324	7	)	)	-RRB-
cord-014597-66vd2mdu	2324	8	.	.	.
cord-014597-66vd2mdu	2325	1	Characterization	characterization	NN
cord-014597-66vd2mdu	2325	2	of	of	IN
cord-014597-66vd2mdu	2325	3	spheroid	spheroid	NN
cord-014597-66vd2mdu	2325	4	"	"	``
cord-014597-66vd2mdu	2325	5	mini	mini	JJ
cord-014597-66vd2mdu	2325	6	bones	bone	NNS
cord-014597-66vd2mdu	2325	7	"	"	''
cord-014597-66vd2mdu	2325	8	.	.	.
cord-014597-66vd2mdu	2326	1	a	a	DT
cord-014597-66vd2mdu	2326	2	Different	different	JJ
cord-014597-66vd2mdu	2326	3	number	number	NN
cord-014597-66vd2mdu	2326	4	(	(	-LRB-
cord-014597-66vd2mdu	2326	5	1.000	1.000	CD
cord-014597-66vd2mdu	2326	6	-	-	SYM
cord-014597-66vd2mdu	2326	7	50.000	50.000	CD
cord-014597-66vd2mdu	2326	8	cells	cell	NNS
cord-014597-66vd2mdu	2326	9	)	)	-RRB-
cord-014597-66vd2mdu	2326	10	of	of	IN
cord-014597-66vd2mdu	2326	11	CI	CI	NNP
cord-014597-66vd2mdu	2326	12	-	-	HYPH
cord-014597-66vd2mdu	2326	13	huOB	huOB	NNP
cord-014597-66vd2mdu	2326	14	cells	cell	NNS
cord-014597-66vd2mdu	2326	15	were	be	VBD
cord-014597-66vd2mdu	2326	16	printed	print	VBN
cord-014597-66vd2mdu	2326	17	into	into	IN
cord-014597-66vd2mdu	2326	18	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2326	19	.	.	.
cord-014597-66vd2mdu	2327	1	b	b	LS
cord-014597-66vd2mdu	2327	2	20.000	20.000	CD
cord-014597-66vd2mdu	2327	3	CI	CI	NNP
cord-014597-66vd2mdu	2327	4	-	-	HYPH
cord-014597-66vd2mdu	2327	5	huOBs	huOBs	NNP
cord-014597-66vd2mdu	2327	6	were	be	VBD
cord-014597-66vd2mdu	2327	7	printed	print	VBN
cord-014597-66vd2mdu	2327	8	into	into	IN
cord-014597-66vd2mdu	2327	9	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2327	10	and	and	CC
cord-014597-66vd2mdu	2327	11	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2327	12	for	for	IN
cord-014597-66vd2mdu	2327	13	indicated	indicate	VBN
cord-014597-66vd2mdu	2327	14	time	time	NN
cord-014597-66vd2mdu	2327	15	points	point	NNS
cord-014597-66vd2mdu	2327	16	.	.	.
cord-014597-66vd2mdu	2328	1	c	c	NNP
cord-014597-66vd2mdu	2329	1	For	for	IN
cord-014597-66vd2mdu	2329	2	analyzing	analyze	VBG
cord-014597-66vd2mdu	2329	3	spheroid	spheroid	NN
cord-014597-66vd2mdu	2329	4	sizes	size	NNS
cord-014597-66vd2mdu	2329	5	,	,	,
cord-014597-66vd2mdu	2329	6	pictures	picture	NNS
cord-014597-66vd2mdu	2329	7	were	be	VBD
cord-014597-66vd2mdu	2329	8	taken	take	VBN
cord-014597-66vd2mdu	2329	9	and	and	CC
cord-014597-66vd2mdu	2329	10	quantified	quantify	VBN
cord-014597-66vd2mdu	2329	11	by	by	IN
cord-014597-66vd2mdu	2330	1	ImageJ.	ImageJ.	NNP
cord-014597-66vd2mdu	2330	2	(	(	-LRB-
cord-014597-66vd2mdu	2330	3	D	D	NNP
cord-014597-66vd2mdu	2330	4	/	/	SYM
cord-014597-66vd2mdu	2330	5	E	e	NN
cord-014597-66vd2mdu	2330	6	)	)	-RRB-
cord-014597-66vd2mdu	2330	7	20.000	20.000	CD
cord-014597-66vd2mdu	2330	8	CI	CI	NNP
cord-014597-66vd2mdu	2330	9	-	-	HYPH
cord-014597-66vd2mdu	2330	10	huOB	huOB	NNP
cord-014597-66vd2mdu	2330	11	cells	cell	NNS
cord-014597-66vd2mdu	2330	12	were	be	VBD
cord-014597-66vd2mdu	2330	13	printed	print	VBN
cord-014597-66vd2mdu	2330	14	into	into	IN
cord-014597-66vd2mdu	2330	15	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2330	16	and	and	CC
cord-014597-66vd2mdu	2330	17	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2330	18	with	with	IN
cord-014597-66vd2mdu	2330	19	(	(	-LRB-
cord-014597-66vd2mdu	2330	20	huOB	huOB	NNP
cord-014597-66vd2mdu	2330	21	Differentiation	Differentiation	NNP
cord-014597-66vd2mdu	2330	22	Medium	Medium	NNP
cord-014597-66vd2mdu	2330	23	)	)	-RRB-
cord-014597-66vd2mdu	2330	24	or	or	CC
cord-014597-66vd2mdu	2330	25	without	without	IN
cord-014597-66vd2mdu	2330	26	a	a	DT
cord-014597-66vd2mdu	2330	27	differentiation	differentiation	NN
cord-014597-66vd2mdu	2330	28	stimulus	stimulus	NN
cord-014597-66vd2mdu	2330	29	for	for	IN
cord-014597-66vd2mdu	2330	30	two	two	CD
cord-014597-66vd2mdu	2330	31	weeks	week	NNS
cord-014597-66vd2mdu	2330	32	.	.	.
cord-014597-66vd2mdu	2331	1	Afterwards	afterwards	RB
cord-014597-66vd2mdu	2331	2	,	,	,
cord-014597-66vd2mdu	2331	3	bioprinted	bioprinted	JJ
cord-014597-66vd2mdu	2331	4	spheroids	spheroid	NNS
cord-014597-66vd2mdu	2331	5	were	be	VBD
cord-014597-66vd2mdu	2331	6	sectioned	section	VBN
cord-014597-66vd2mdu	2331	7	by	by	IN
cord-014597-66vd2mdu	2331	8	a	a	DT
cord-014597-66vd2mdu	2331	9	cryo	cryo	NN
cord-014597-66vd2mdu	2331	10	microtome	microtome	NN
cord-014597-66vd2mdu	2331	11	and	and	CC
cord-014597-66vd2mdu	2331	12	d	d	LS
cord-014597-66vd2mdu	2331	13	stained	stain	VBN
cord-014597-66vd2mdu	2331	14	for	for	IN
cord-014597-66vd2mdu	2331	15	Ca	Ca	NNP
cord-014597-66vd2mdu	2331	16	2	2	CD
cord-014597-66vd2mdu	2331	17	+	+	SYM
cord-014597-66vd2mdu	2331	18	deposits	deposit	NNS
cord-014597-66vd2mdu	2331	19	(	(	-LRB-
cord-014597-66vd2mdu	2331	20	Alizarin	Alizarin	NNP
cord-014597-66vd2mdu	2331	21	Red	Red	NNP
cord-014597-66vd2mdu	2331	22	S	S	NNP
cord-014597-66vd2mdu	2331	23	)	)	-RRB-
cord-014597-66vd2mdu	2331	24	or	or	CC
cord-014597-66vd2mdu	2331	25	e	e	LS
cord-014597-66vd2mdu	2331	26	stained	stain	VBN
cord-014597-66vd2mdu	2331	27	for	for	IN
cord-014597-66vd2mdu	2331	28	alkaline	alkaline	JJ
cord-014597-66vd2mdu	2331	29	phosphatase	phosphatase	NN
cord-014597-66vd2mdu	2331	30	activity	activity	NN
cord-014597-66vd2mdu	2331	31	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	2331	32	/	/	,
cord-014597-66vd2mdu	2331	33	cas9	cas9	XX
cord-014597-66vd2mdu	2331	34	,	,	,
cord-014597-66vd2mdu	2331	35	a	a	DT
cord-014597-66vd2mdu	2331	36	novel	novel	JJ
cord-014597-66vd2mdu	2331	37	genomic	genomic	JJ
cord-014597-66vd2mdu	2331	38	tool	tool	NN
cord-014597-66vd2mdu	2331	39	to	to	TO
cord-014597-66vd2mdu	2331	40	knock	knock	VB
cord-014597-66vd2mdu	2331	41	down	down	RP
cord-014597-66vd2mdu	2331	42	microRNA	microRNA	NNP
cord-014597-66vd2mdu	2331	43	in	in	IN
cord-014597-66vd2mdu	2331	44	vitro	vitro	FW
cord-014597-66vd2mdu	2331	45	and	and	CC
cord-014597-66vd2mdu	2331	46	in	in	IN
cord-014597-66vd2mdu	2331	47	vivo	vivo	NNP
cord-014597-66vd2mdu	2331	48	Degrontagged	degrontagge	VBN
cord-014597-66vd2mdu	2331	49	dCas9	dCas9	NNP
cord-014597-66vd2mdu	2331	50	/	/	SYM
cord-014597-66vd2mdu	2331	51	Cpf1	Cpf1	NNP
cord-014597-66vd2mdu	2331	52	Effectors	Effectors	NNP
cord-014597-66vd2mdu	2331	53	for	for	IN
cord-014597-66vd2mdu	2331	54	Multi	multi	JJ
cord-014597-66vd2mdu	2331	55	-	-	JJ
cord-014597-66vd2mdu	2331	56	directional	directional	JJ
cord-014597-66vd2mdu	2331	57	Drug	drug	NN
cord-014597-66vd2mdu	2331	58	-	-	HYPH
cord-014597-66vd2mdu	2331	59	inducible	inducible	JJ
cord-014597-66vd2mdu	2331	60	control	control	NN
cord-014597-66vd2mdu	2331	61	of	of	IN
cord-014597-66vd2mdu	2331	62	Synthetic	Synthetic	NNP
cord-014597-66vd2mdu	2331	63	Gene	Gene	NNP
cord-014597-66vd2mdu	2331	64	Regulation	Regulation	NNP
cord-014597-66vd2mdu	2331	65	Assessing	assess	VBG
cord-014597-66vd2mdu	2331	66	the	the	DT
cord-014597-66vd2mdu	2331	67	Variability	Variability	NNP
cord-014597-66vd2mdu	2331	68	of	of	IN
cord-014597-66vd2mdu	2331	69	an	an	DT
cord-014597-66vd2mdu	2331	70	Innovator	Innovator	NNP
cord-014597-66vd2mdu	2331	71	Molecule	Molecule	NNP
cord-014597-66vd2mdu	2331	72	N	N	NNP
cord-014597-66vd2mdu	2331	73	-	-	HYPH
cord-014597-66vd2mdu	2331	74	Glycan	Glycan	NNP
cord-014597-66vd2mdu	2331	75	Profile	Profile	NNP
cord-014597-66vd2mdu	2331	76	Correct	correct	VB
cord-014597-66vd2mdu	2331	77	primary	primary	JJ
cord-014597-66vd2mdu	2331	78	structure	structure	NN
cord-014597-66vd2mdu	2331	79	assessment	assessment	NN
cord-014597-66vd2mdu	2331	80	and	and	CC
cord-014597-66vd2mdu	2331	81	extensive	extensive	JJ
cord-014597-66vd2mdu	2331	82	glyco	glyco	NNP
cord-014597-66vd2mdu	2331	83	-	-	HYPH
cord-014597-66vd2mdu	2331	84	profiling	profiling	NN
cord-014597-66vd2mdu	2331	85	of	of	IN
cord-014597-66vd2mdu	2331	86	cetuximab	cetuximab	NNS
cord-014597-66vd2mdu	2331	87	by	by	IN
cord-014597-66vd2mdu	2331	88	a	a	DT
cord-014597-66vd2mdu	2331	89	combination	combination	NN
cord-014597-66vd2mdu	2331	90	of	of	IN
cord-014597-66vd2mdu	2331	91	intact	intact	JJ
cord-014597-66vd2mdu	2331	92	,	,	,
cord-014597-66vd2mdu	2331	93	middle	middle	NN
cord-014597-66vd2mdu	2331	94	-	-	HYPH
cord-014597-66vd2mdu	2331	95	up	up	NN
cord-014597-66vd2mdu	2331	96	,	,	,
cord-014597-66vd2mdu	2331	97	middle	middle	NN
cord-014597-66vd2mdu	2331	98	-	-	HYPH
cord-014597-66vd2mdu	2331	99	down	down	NN
cord-014597-66vd2mdu	2331	100	and	and	CC
cord-014597-66vd2mdu	2331	101	bottom	bottom	NN
cord-014597-66vd2mdu	2331	102	-	-	HYPH
cord-014597-66vd2mdu	2331	103	up	up	NN
cord-014597-66vd2mdu	2331	104	ESI	ESI	NNP
cord-014597-66vd2mdu	2331	105	and	and	CC
cord-014597-66vd2mdu	2331	106	MALDI	MALDI	NNP
cord-014597-66vd2mdu	2331	107	mass	mass	NN
cord-014597-66vd2mdu	2331	108	spectrometry	spectrometry	NN
cord-014597-66vd2mdu	2331	109	techniques	technique	NNS
cord-014597-66vd2mdu	2331	110	2D	2D	NNP
cord-014597-66vd2mdu	2331	111	-	-	HYPH
cord-014597-66vd2mdu	2331	112	DIGE	dige	NN
cord-014597-66vd2mdu	2331	113	screening	screening	NN
cord-014597-66vd2mdu	2331	114	of	of	IN
cord-014597-66vd2mdu	2331	115	high	high	JJ
cord-014597-66vd2mdu	2331	116	productive	productive	JJ
cord-014597-66vd2mdu	2331	117	CHO	CHO	NNP
cord-014597-66vd2mdu	2331	118	cells	cell	NNS
cord-014597-66vd2mdu	2331	119	under	under	IN
cord-014597-66vd2mdu	2331	120	glucoselimitation	glucoselimitation	NN
cord-014597-66vd2mdu	2331	121	-basic	-basic	HYPH
cord-014597-66vd2mdu	2331	122	changes	change	NNS
cord-014597-66vd2mdu	2331	123	in	in	IN
cord-014597-66vd2mdu	2331	124	the	the	DT
cord-014597-66vd2mdu	2331	125	proteome	proteome	NN
cord-014597-66vd2mdu	2331	126	equipment	equipment	NN
cord-014597-66vd2mdu	2331	127	and	and	CC
cord-014597-66vd2mdu	2331	128	hints	hint	NNS
cord-014597-66vd2mdu	2331	129	for	for	IN
cord-014597-66vd2mdu	2331	130	epigenetic	epigenetic	JJ
cord-014597-66vd2mdu	2331	131	effects	effect	NNS
cord-014597-66vd2mdu	2331	132	Dependence	dependence	NN
cord-014597-66vd2mdu	2331	133	on	on	IN
cord-014597-66vd2mdu	2331	134	Glucose	Glucose	NNP
cord-014597-66vd2mdu	2331	135	Limitation	Limitation	NNP
cord-014597-66vd2mdu	2331	136	of	of	IN
cord-014597-66vd2mdu	2331	137	the	the	DT
cord-014597-66vd2mdu	2331	138	pCO2	pCO2	NNP
cord-014597-66vd2mdu	2331	139	Influences	Influences	NNPS
cord-014597-66vd2mdu	2331	140	on	on	IN
cord-014597-66vd2mdu	2331	141	CHO	CHO	NNP
cord-014597-66vd2mdu	2331	142	Cell	Cell	NNP
cord-014597-66vd2mdu	2331	143	Growth	Growth	NNP
cord-014597-66vd2mdu	2331	144	,	,	,
cord-014597-66vd2mdu	2331	145	Metabolism	metabolism	NN
cord-014597-66vd2mdu	2331	146	and	and	CC
cord-014597-66vd2mdu	2331	147	IgG	IgG	NNP
cord-014597-66vd2mdu	2331	148	Production	Production	NNP
cord-014597-66vd2mdu	2331	149	Fcgalactosylation	Fcgalactosylation	NNP
cord-014597-66vd2mdu	2331	150	modulates	modulate	VBZ
cord-014597-66vd2mdu	2331	151	antibody	antibody	NN
cord-014597-66vd2mdu	2331	152	-	-	HYPH
cord-014597-66vd2mdu	2331	153	dependent	dependent	JJ
cord-014597-66vd2mdu	2331	154	cellular	cellular	JJ
cord-014597-66vd2mdu	2331	155	cytotoxicity	cytotoxicity	NN
cord-014597-66vd2mdu	2331	156	of	of	IN
cord-014597-66vd2mdu	2331	157	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2331	158	antibodies	antibody	NNS
cord-014597-66vd2mdu	2332	1	Fc	Fc	NNP
cord-014597-66vd2mdu	2332	2	glycans	glycan	NNS
cord-014597-66vd2mdu	2332	3	of	of	IN
cord-014597-66vd2mdu	2332	4	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2332	5	antibodies	antibody	NNS
cord-014597-66vd2mdu	2332	6	as	as	IN
cord-014597-66vd2mdu	2332	7	critical	critical	JJ
cord-014597-66vd2mdu	2332	8	quality	quality	NN
cord-014597-66vd2mdu	2332	9	attributes	attribute	VBZ
cord-014597-66vd2mdu	2332	10	Development	Development	NNP
cord-014597-66vd2mdu	2332	11	of	of	IN
cord-014597-66vd2mdu	2332	12	an	an	DT
cord-014597-66vd2mdu	2332	13	automated	automated	JJ
cord-014597-66vd2mdu	2332	14	,	,	,
cord-014597-66vd2mdu	2332	15	multiwell	multiwell	NNP
cord-014597-66vd2mdu	2332	16	plate	plate	NN
cord-014597-66vd2mdu	2332	17	based	base	VBN
cord-014597-66vd2mdu	2332	18	screening	screening	NN
cord-014597-66vd2mdu	2332	19	system	system	NN
cord-014597-66vd2mdu	2332	20	for	for	IN
cord-014597-66vd2mdu	2332	21	suspension	suspension	NN
cord-014597-66vd2mdu	2332	22	cell	cell	NN
cord-014597-66vd2mdu	2332	23	culture	culture	NN
cord-014597-66vd2mdu	2333	1	Global	global	JJ
cord-014597-66vd2mdu	2333	2	cancer	cancer	NN
cord-014597-66vd2mdu	2333	3	statistics	statistic	NNS
cord-014597-66vd2mdu	2333	4	Remission	remission	NN
cord-014597-66vd2mdu	2333	5	of	of	IN
cord-014597-66vd2mdu	2333	6	disseminated	disseminate	VBN
cord-014597-66vd2mdu	2333	7	cancer	cancer	NN
cord-014597-66vd2mdu	2333	8	after	after	IN
cord-014597-66vd2mdu	2333	9	systemic	systemic	JJ
cord-014597-66vd2mdu	2333	10	oncolytic	oncolytic	VBN
cord-014597-66vd2mdu	2333	11	virotherapy	virotherapy	NN
cord-014597-66vd2mdu	2334	1	Screening	screen	VBG
cord-014597-66vd2mdu	2334	2	different	different	JJ
cord-014597-66vd2mdu	2334	3	host	host	NN
cord-014597-66vd2mdu	2334	4	cell	cell	NN
cord-014597-66vd2mdu	2334	5	lines	line	NNS
cord-014597-66vd2mdu	2334	6	for	for	IN
cord-014597-66vd2mdu	2334	7	the	the	DT
cord-014597-66vd2mdu	2334	8	dynamic	dynamic	JJ
cord-014597-66vd2mdu	2334	9	production	production	NN
cord-014597-66vd2mdu	2334	10	of	of	IN
cord-014597-66vd2mdu	2334	11	measles	measle	NNS
cord-014597-66vd2mdu	2334	12	virus	virus	NN
cord-014597-66vd2mdu	2335	1	Beitrag	Beitrag	NNP
cord-014597-66vd2mdu	2335	2	zur	zur	NNP
cord-014597-66vd2mdu	2335	3	kollektiven	kollektiven	NNP
cord-014597-66vd2mdu	2335	4	Behandlung	Behandlung	NNP
cord-014597-66vd2mdu	2335	5	pharmakologischer	pharmakologischer	NNP
cord-014597-66vd2mdu	2336	1	Reihenversuche	reihenversuche	NN
cord-014597-66vd2mdu	2337	1	A	a	DT
cord-014597-66vd2mdu	2337	2	simple	simple	JJ
cord-014597-66vd2mdu	2337	3	method	method	NN
cord-014597-66vd2mdu	2337	4	of	of	IN
cord-014597-66vd2mdu	2337	5	estimating	estimate	VBG
cord-014597-66vd2mdu	2337	6	fifty	fifty	CD
cord-014597-66vd2mdu	2337	7	per	per	IN
cord-014597-66vd2mdu	2337	8	cent	cent	NN
cord-014597-66vd2mdu	2337	9	endpoints	endpoint	NNS
cord-014597-66vd2mdu	2337	10	Webinar	Webinar	NNP
cord-014597-66vd2mdu	2337	11	:	:	:
cord-014597-66vd2mdu	2337	12	ambr15	ambr15	NN
cord-014597-66vd2mdu	2337	13	as	as	IN
cord-014597-66vd2mdu	2337	14	a	a	DT
cord-014597-66vd2mdu	2337	15	sedimentation	sedimentation	NN
cord-014597-66vd2mdu	2337	16	-	-	HYPH
cord-014597-66vd2mdu	2337	17	perfusion	perfusion	NN
cord-014597-66vd2mdu	2337	18	model	model	NN
cord-014597-66vd2mdu	2337	19	for	for	IN
cord-014597-66vd2mdu	2337	20	cultivation	cultivation	NN
cord-014597-66vd2mdu	2337	21	characteristics	characteristic	NNS
cord-014597-66vd2mdu	2337	22	and	and	CC
cord-014597-66vd2mdu	2337	23	product	product	NN
cord-014597-66vd2mdu	2337	24	quality	quality	NN
cord-014597-66vd2mdu	2337	25	prediction	prediction	NN
cord-014597-66vd2mdu	2337	26	De	De	NNP
cord-014597-66vd2mdu	2337	27	novo	novo	NNP
cord-014597-66vd2mdu	2337	28	"	"	``
cord-014597-66vd2mdu	2337	29	high	high	JJ
cord-014597-66vd2mdu	2337	30	density	density	NN
cord-014597-66vd2mdu	2337	31	perfusion	perfusion	NN
cord-014597-66vd2mdu	2337	32	medium	medium	NN
cord-014597-66vd2mdu	2337	33	:	:	:
cord-014597-66vd2mdu	2337	34	increased	increase	VBN
cord-014597-66vd2mdu	2337	35	productivity	productivity	NN
cord-014597-66vd2mdu	2337	36	and	and	CC
cord-014597-66vd2mdu	2337	37	reduced	reduce	VBN
cord-014597-66vd2mdu	2337	38	perfusion	perfusion	NN
cord-014597-66vd2mdu	2337	39	rates	rate	NNS
cord-014597-66vd2mdu	2338	1	A	a	DT
cord-014597-66vd2mdu	2338	2	high	high	RB
cord-014597-66vd2mdu	2338	3	-	-	HYPH
cord-014597-66vd2mdu	2338	4	yielding	yield	VBG
cord-014597-66vd2mdu	2338	5	CHO	CHO	NNP
cord-014597-66vd2mdu	2338	6	transient	transient	JJ
cord-014597-66vd2mdu	2338	7	system	system	NN
cord-014597-66vd2mdu	2338	8	:	:	:
cord-014597-66vd2mdu	2338	9	Co	co	NN
cord-014597-66vd2mdu	2338	10	-	-	NN
cord-014597-66vd2mdu	2338	11	expression	expression	NN
cord-014597-66vd2mdu	2338	12	of	of	IN
cord-014597-66vd2mdu	2338	13	genes	gene	NNS
cord-014597-66vd2mdu	2338	14	encoding	encode	VBG
cord-014597-66vd2mdu	2338	15	EBNA-1	EBNA-1	NNP
cord-014597-66vd2mdu	2338	16	and	and	CC
cord-014597-66vd2mdu	2338	17	GS	GS	NNP
cord-014597-66vd2mdu	2338	18	enhances	enhance	VBZ
cord-014597-66vd2mdu	2338	19	transient	transient	JJ
cord-014597-66vd2mdu	2338	20	protein	protein	NN
cord-014597-66vd2mdu	2338	21	expression	expression	NN
cord-014597-66vd2mdu	2339	1	An	an	DT
cord-014597-66vd2mdu	2339	2	integrated	integrated	JJ
cord-014597-66vd2mdu	2339	3	vector	vector	NN
cord-014597-66vd2mdu	2339	4	system	system	NN
cord-014597-66vd2mdu	2339	5	for	for	IN
cord-014597-66vd2mdu	2339	6	the	the	DT
cord-014597-66vd2mdu	2339	7	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	2339	8	expression	expression	NN
cord-014597-66vd2mdu	2339	9	of	of	IN
cord-014597-66vd2mdu	2339	10	antibodies	antibody	NNS
cord-014597-66vd2mdu	2339	11	or	or	CC
cord-014597-66vd2mdu	2339	12	their	-PRON-	PRP$
cord-014597-66vd2mdu	2339	13	fragments	fragment	NNS
cord-014597-66vd2mdu	2339	14	after	after	IN
cord-014597-66vd2mdu	2339	15	selection	selection	NN
cord-014597-66vd2mdu	2339	16	from	from	IN
cord-014597-66vd2mdu	2339	17	phage	phage	NN
cord-014597-66vd2mdu	2339	18	display	display	NN
cord-014597-66vd2mdu	2339	19	libraries	library	NNS
cord-014597-66vd2mdu	2339	20	Towards	towards	IN
cord-014597-66vd2mdu	2339	21	the	the	DT
cord-014597-66vd2mdu	2339	22	development	development	NN
cord-014597-66vd2mdu	2339	23	of	of	IN
cord-014597-66vd2mdu	2339	24	a	a	DT
cord-014597-66vd2mdu	2339	25	surface	surface	NN
cord-014597-66vd2mdu	2339	26	plasmon	plasmon	NN
cord-014597-66vd2mdu	2339	27	resonance	resonance	NN
cord-014597-66vd2mdu	2339	28	assay	assay	NN
cord-014597-66vd2mdu	2339	29	to	to	TO
cord-014597-66vd2mdu	2339	30	evaluate	evaluate	VB
cord-014597-66vd2mdu	2339	31	the	the	DT
cord-014597-66vd2mdu	2339	32	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	2339	33	pattern	pattern	NN
cord-014597-66vd2mdu	2339	34	of	of	IN
cord-014597-66vd2mdu	2339	35	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	2339	36	antibodies	antibody	NNS
cord-014597-66vd2mdu	2339	37	using	use	VBG
cord-014597-66vd2mdu	2339	38	the	the	DT
cord-014597-66vd2mdu	2339	39	extracellular	extracellular	JJ
cord-014597-66vd2mdu	2339	40	domains	domain	NNS
cord-014597-66vd2mdu	2339	41	of	of	IN
cord-014597-66vd2mdu	2339	42	CD16a	CD16a	NNP
cord-014597-66vd2mdu	2339	43	and	and	CC
cord-014597-66vd2mdu	2339	44	CD64	CD64	NNP
cord-014597-66vd2mdu	2339	45	Biotinylation	Biotinylation	NNP
cord-014597-66vd2mdu	2339	46	of	of	IN
cord-014597-66vd2mdu	2339	47	the	the	DT
cord-014597-66vd2mdu	2339	48	Fc	Fc	NNP
cord-014597-66vd2mdu	2339	49	gamma	gamma	NN
cord-014597-66vd2mdu	2339	50	receptor	receptor	NN
cord-014597-66vd2mdu	2339	51	ectodomains	ectodomain	NNS
cord-014597-66vd2mdu	2339	52	by	by	IN
cord-014597-66vd2mdu	2339	53	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2339	54	cell	cell	NN
cord-014597-66vd2mdu	2339	55	co	co	NN
cord-014597-66vd2mdu	2339	56	-	-	NN
cord-014597-66vd2mdu	2339	57	transfection	transfection	JJ
cord-014597-66vd2mdu	2339	58	:	:	:
cord-014597-66vd2mdu	2339	59	application	application	NN
cord-014597-66vd2mdu	2339	60	to	to	IN
cord-014597-66vd2mdu	2339	61	the	the	DT
cord-014597-66vd2mdu	2339	62	development	development	NN
cord-014597-66vd2mdu	2339	63	of	of	IN
cord-014597-66vd2mdu	2339	64	a	a	DT
cord-014597-66vd2mdu	2339	65	surface	surface	NN
cord-014597-66vd2mdu	2339	66	plasmon	plasmon	JJ
cord-014597-66vd2mdu	2339	67	resonance	resonance	NN
cord-014597-66vd2mdu	2339	68	-	-	HYPH
cord-014597-66vd2mdu	2339	69	based	base	VBN
cord-014597-66vd2mdu	2339	70	assay	assay	NN
cord-014597-66vd2mdu	2339	71	ambr	ambr	NNP
cord-014597-66vd2mdu	2339	72	™	™	NNP
cord-014597-66vd2mdu	2339	73	Mini	mini	JJ
cord-014597-66vd2mdu	2339	74	-	-	HYPH
cord-014597-66vd2mdu	2339	75	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2339	76	as	as	IN
cord-014597-66vd2mdu	2339	77	a	a	DT
cord-014597-66vd2mdu	2339	78	High	High	NNP
cord-014597-66vd2mdu	2339	79	-	-	HYPH
cord-014597-66vd2mdu	2339	80	Throughput	throughput	NN
cord-014597-66vd2mdu	2339	81	Tool	tool	NN
cord-014597-66vd2mdu	2339	82	for	for	IN
cord-014597-66vd2mdu	2339	83	Culture	Culture	NNP
cord-014597-66vd2mdu	2339	84	Process	Process	NNP
cord-014597-66vd2mdu	2339	85	Development	Development	NNP
cord-014597-66vd2mdu	2339	86	to	to	TO
cord-014597-66vd2mdu	2339	87	Accelerate	accelerate	VB
cord-014597-66vd2mdu	2339	88	Transfer	Transfer	NNP
cord-014597-66vd2mdu	2339	89	to	to	IN
cord-014597-66vd2mdu	2339	90	Stainless	Stainless	NNP
cord-014597-66vd2mdu	2339	91	Steel	Steel	NNP
cord-014597-66vd2mdu	2339	92	Manufacturing	Manufacturing	NNP
cord-014597-66vd2mdu	2339	93	Scale	scale	NN
cord-014597-66vd2mdu	2340	1	:	:	:
cord-014597-66vd2mdu	2340	2	Comparability	comparability	NN
cord-014597-66vd2mdu	2340	3	study	study	NN
cord-014597-66vd2mdu	2340	4	from	from	IN
cord-014597-66vd2mdu	2340	5	Process	Process	NNP
cord-014597-66vd2mdu	2340	6	Performance	Performance	NNP
cord-014597-66vd2mdu	2340	7	to	to	IN
cord-014597-66vd2mdu	2340	8	Product	Product	NNP
cord-014597-66vd2mdu	2340	9	Quality	quality	NN
cord-014597-66vd2mdu	2340	10	Attributes	Attributes	NNPS
cord-014597-66vd2mdu	2340	11	Maximizing	maximize	VBG
cord-014597-66vd2mdu	2340	12	binding	bind	VBG
cord-014597-66vd2mdu	2340	13	capacity	capacity	NN
cord-014597-66vd2mdu	2340	14	for	for	IN
cord-014597-66vd2mdu	2340	15	protein	protein	NN
cord-014597-66vd2mdu	2340	16	A	a	DT
cord-014597-66vd2mdu	2340	17	chromatography	chromatography	NN
cord-014597-66vd2mdu	2340	18	Protein	Protein	NNP
cord-014597-66vd2mdu	2340	19	Glycosylation	Glycosylation	NNP
cord-014597-66vd2mdu	2340	20	and	and	CC
cord-014597-66vd2mdu	2340	21	its	-PRON-	PRP$
cord-014597-66vd2mdu	2340	22	Role	role	NN
cord-014597-66vd2mdu	2340	23	in	in	IN
cord-014597-66vd2mdu	2340	24	Protein	Protein	NNP
cord-014597-66vd2mdu	2340	25	Folding	Folding	NNP
cord-014597-66vd2mdu	2340	26	Afucosylated	Afucosylated	NNP
cord-014597-66vd2mdu	2340	27	antibodies	antibody	NNS
cord-014597-66vd2mdu	2340	28	increase	increase	VBP
cord-014597-66vd2mdu	2340	29	activation	activation	NN
cord-014597-66vd2mdu	2340	30	of	of	IN
cord-014597-66vd2mdu	2340	31	FcγRIIIa	fcγriiia	NN
cord-014597-66vd2mdu	2340	32	-	-	HYPH
cord-014597-66vd2mdu	2340	33	dependent	dependent	JJ
cord-014597-66vd2mdu	2340	34	signaling	signal	VBG
cord-014597-66vd2mdu	2340	35	components	component	NNS
cord-014597-66vd2mdu	2340	36	to	to	TO
cord-014597-66vd2mdu	2340	37	intensify	intensify	VB
cord-014597-66vd2mdu	2340	38	processes	process	NNS
cord-014597-66vd2mdu	2340	39	promoting	promote	VBG
cord-014597-66vd2mdu	2340	40	ADCC	ADCC	NNP
cord-014597-66vd2mdu	2340	41	Sialic	Sialic	NNP
cord-014597-66vd2mdu	2340	42	Acids	Acids	NNPS
cord-014597-66vd2mdu	2340	43	and	and	CC
cord-014597-66vd2mdu	2340	44	other	other	JJ
cord-014597-66vd2mdu	2340	45	Nonulosonic	Nonulosonic	NNP
cord-014597-66vd2mdu	2340	46	Acids	Acids	NNPS
cord-014597-66vd2mdu	2340	47	Production	Production	NNP
cord-014597-66vd2mdu	2340	48	of	of	IN
cord-014597-66vd2mdu	2340	49	antibody	antibody	NN
cord-014597-66vd2mdu	2340	50	in	in	IN
cord-014597-66vd2mdu	2340	51	insect	insect	NN
cord-014597-66vd2mdu	2340	52	cells	cell	NNS
cord-014597-66vd2mdu	2340	53	Suitability	suitability	NN
cord-014597-66vd2mdu	2340	54	and	and	CC
cord-014597-66vd2mdu	2340	55	perspectives	perspective	NNS
cord-014597-66vd2mdu	2340	56	on	on	IN
cord-014597-66vd2mdu	2340	57	using	use	VBG
cord-014597-66vd2mdu	2340	58	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2340	59	insect	insect	NN
cord-014597-66vd2mdu	2340	60	cells	cell	NNS
cord-014597-66vd2mdu	2340	61	for	for	IN
cord-014597-66vd2mdu	2340	62	the	the	DT
cord-014597-66vd2mdu	2340	63	production	production	NN
cord-014597-66vd2mdu	2340	64	of	of	IN
cord-014597-66vd2mdu	2340	65	virus	virus	NN
cord-014597-66vd2mdu	2340	66	-	-	HYPH
cord-014597-66vd2mdu	2340	67	like	like	JJ
cord-014597-66vd2mdu	2340	68	particles	particle	NNS
cord-014597-66vd2mdu	2340	69	Cloning	clone	VBG
cord-014597-66vd2mdu	2340	70	of	of	IN
cord-014597-66vd2mdu	2340	71	cDNA	cdna	NN
cord-014597-66vd2mdu	2340	72	and	and	CC
cord-014597-66vd2mdu	2340	73	characterization	characterization	NN
cord-014597-66vd2mdu	2340	74	of	of	IN
cord-014597-66vd2mdu	2340	75	anti	anti	JJ
cord-014597-66vd2mdu	2340	76	-	-	JJ
cord-014597-66vd2mdu	2340	77	RNase	rnase	JJ
cord-014597-66vd2mdu	2340	78	A	a	DT
cord-014597-66vd2mdu	2340	79	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	2340	80	antibody	antibody	NN
cord-014597-66vd2mdu	2340	81	3A21	3a21	CD
cord-014597-66vd2mdu	2340	82	Production	production	NN
cord-014597-66vd2mdu	2340	83	of	of	IN
cord-014597-66vd2mdu	2340	84	functional	functional	JJ
cord-014597-66vd2mdu	2340	85	antibody	antibody	NN
cord-014597-66vd2mdu	2340	86	Fab	fab	JJ
cord-014597-66vd2mdu	2340	87	fragment	fragment	NN
cord-014597-66vd2mdu	2340	88	by	by	IN
cord-014597-66vd2mdu	2340	89	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2340	90	insect	insect	NN
cord-014597-66vd2mdu	2340	91	cells	cell	NNS
cord-014597-66vd2mdu	2340	92	Optimization	optimization	NN
cord-014597-66vd2mdu	2340	93	of	of	IN
cord-014597-66vd2mdu	2340	94	HEK-293S	hek-293s	JJ
cord-014597-66vd2mdu	2340	95	cell	cell	NN
cord-014597-66vd2mdu	2340	96	cultures	culture	NNS
cord-014597-66vd2mdu	2340	97	for	for	IN
cord-014597-66vd2mdu	2340	98	the	the	DT
cord-014597-66vd2mdu	2340	99	production	production	NN
cord-014597-66vd2mdu	2340	100	of	of	IN
cord-014597-66vd2mdu	2340	101	adenoviral	adenoviral	JJ
cord-014597-66vd2mdu	2340	102	vectors	vector	NNS
cord-014597-66vd2mdu	2340	103	in	in	IN
cord-014597-66vd2mdu	2340	104	bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	2340	105	using	use	VBG
cord-014597-66vd2mdu	2340	106	on	on	IN
cord-014597-66vd2mdu	2340	107	-	-	HYPH
cord-014597-66vd2mdu	2340	108	line	line	NN
cord-014597-66vd2mdu	2340	109	OUR	our	PRP$
cord-014597-66vd2mdu	2340	110	measurements	measurement	NNS
cord-014597-66vd2mdu	2340	111	Enhancing	enhance	VBG
cord-014597-66vd2mdu	2340	112	heterologous	heterologous	JJ
cord-014597-66vd2mdu	2340	113	protein	protein	NN
cord-014597-66vd2mdu	2340	114	expression	expression	NN
cord-014597-66vd2mdu	2340	115	and	and	CC
cord-014597-66vd2mdu	2340	116	secretion	secretion	NN
cord-014597-66vd2mdu	2340	117	in	in	IN
cord-014597-66vd2mdu	2340	118	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2340	119	cells	cell	NNS
cord-014597-66vd2mdu	2340	120	by	by	IN
cord-014597-66vd2mdu	2340	121	means	mean	NNS
cord-014597-66vd2mdu	2340	122	of	of	IN
cord-014597-66vd2mdu	2340	123	combination	combination	NN
cord-014597-66vd2mdu	2340	124	of	of	IN
cord-014597-66vd2mdu	2340	125	CMV	CMV	NNP
cord-014597-66vd2mdu	2340	126	promoter	promoter	NN
cord-014597-66vd2mdu	2340	127	and	and	CC
cord-014597-66vd2mdu	2340	128	IFNα2	ifnα2	JJ
cord-014597-66vd2mdu	2340	129	signal	signal	NN
cord-014597-66vd2mdu	2340	130	peptide	peptide	NN
cord-014597-66vd2mdu	2340	131	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2340	132	cell	cell	NN
cord-014597-66vd2mdu	2340	133	culture	culture	NN
cord-014597-66vd2mdu	2340	134	media	medium	NNS
cord-014597-66vd2mdu	2340	135	study	study	NN
cord-014597-66vd2mdu	2340	136	towards	towards	IN
cord-014597-66vd2mdu	2340	137	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	2340	138	optimization	optimization	NN
cord-014597-66vd2mdu	2340	139	:	:	:
cord-014597-66vd2mdu	2340	140	animal	animal	NN
cord-014597-66vd2mdu	2340	141	derived	derive	VBN
cord-014597-66vd2mdu	2340	142	component	component	NN
cord-014597-66vd2mdu	2340	143	free	free	JJ
cord-014597-66vd2mdu	2340	144	and	and	CC
cord-014597-66vd2mdu	2340	145	animal	animal	NN
cord-014597-66vd2mdu	2340	146	derived	derive	VBN
cord-014597-66vd2mdu	2340	147	component	component	NN
cord-014597-66vd2mdu	2340	148	containing	contain	VBG
cord-014597-66vd2mdu	2340	149	platforms	platform	NNS
cord-014597-66vd2mdu	2340	150	Comparison	comparison	NN
cord-014597-66vd2mdu	2340	151	of	of	IN
cord-014597-66vd2mdu	2340	152	control	control	NN
cord-014597-66vd2mdu	2340	153	strategies	strategy	NNS
cord-014597-66vd2mdu	2340	154	for	for	IN
cord-014597-66vd2mdu	2340	155	fed	fed	JJ
cord-014597-66vd2mdu	2340	156	-	-	HYPH
cord-014597-66vd2mdu	2340	157	batch	batch	NN
cord-014597-66vd2mdu	2340	158	culture	culture	NN
cord-014597-66vd2mdu	2340	159	of	of	IN
cord-014597-66vd2mdu	2340	160	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	2340	161	cells	cell	NNS
cord-014597-66vd2mdu	2340	162	based	base	VBN
cord-014597-66vd2mdu	2340	163	on	on	IN
cord-014597-66vd2mdu	2340	164	on	on	IN
cord-014597-66vd2mdu	2340	165	-	-	HYPH
cord-014597-66vd2mdu	2340	166	line	line	NN
cord-014597-66vd2mdu	2340	167	monitoring	monitoring	NN
cord-014597-66vd2mdu	2340	168	of	of	IN
cord-014597-66vd2mdu	2340	169	oxygen	oxygen	NN
cord-014597-66vd2mdu	2340	170	uptake	uptake	NN
cord-014597-66vd2mdu	2340	171	rate	rate	NN
cord-014597-66vd2mdu	2340	172	,	,	,
cord-014597-66vd2mdu	2340	173	optical	optical	JJ
cord-014597-66vd2mdu	2340	174	cell	cell	NN
cord-014597-66vd2mdu	2340	175	density	density	NN
cord-014597-66vd2mdu	2340	176	and	and	CC
cord-014597-66vd2mdu	2340	177	glucose	glucose	NN
cord-014597-66vd2mdu	2340	178	concentration	concentration	NN
cord-014597-66vd2mdu	2340	179	Continuous	Continuous	NNP
cord-014597-66vd2mdu	2340	180	bioprocessing	bioprocessing	NN
cord-014597-66vd2mdu	2340	181	:	:	:
cord-014597-66vd2mdu	2340	182	The	the	DT
cord-014597-66vd2mdu	2340	183	real	real	JJ
cord-014597-66vd2mdu	2340	184	thing	thing	NN
cord-014597-66vd2mdu	2340	185	this	this	DT
cord-014597-66vd2mdu	2340	186	time	time	NN
cord-014597-66vd2mdu	2340	187	?	?	.
cord-014597-66vd2mdu	2341	1	mAbs	mAbs	NNP
cord-014597-66vd2mdu	2341	2	White	white	JJ
cord-014597-66vd2mdu	2341	3	paper	paper	NN
cord-014597-66vd2mdu	2341	4	on	on	IN
cord-014597-66vd2mdu	2341	5	continuous	continuous	JJ
cord-014597-66vd2mdu	2341	6	bioprocessing	bioprocesse	VBG
cord-014597-66vd2mdu	2341	7	FDA	FDA	NNP
cord-014597-66vd2mdu	2341	8	Perspective	Perspective	NNP
cord-014597-66vd2mdu	2341	9	on	on	IN
cord-014597-66vd2mdu	2341	10	Continuous	Continuous	NNP
cord-014597-66vd2mdu	2341	11	Manufacturing	Manufacturing	NNP
cord-014597-66vd2mdu	2341	12	.	.	.
cord-014597-66vd2mdu	2342	1	IFPAC	IFPAC	NNP
cord-014597-66vd2mdu	2342	2	Annu	Annu	NNP
cord-014597-66vd2mdu	2342	3	.	.	.
cord-014597-66vd2mdu	2343	1	Meet	meet	VB
cord-014597-66vd2mdu	2343	2	Screening	Screening	NNP
cord-014597-66vd2mdu	2343	3	and	and	CC
cord-014597-66vd2mdu	2343	4	Assessment	Assessment	NNP
cord-014597-66vd2mdu	2343	5	of	of	IN
cord-014597-66vd2mdu	2343	6	Performance	Performance	NNP
cord-014597-66vd2mdu	2343	7	and	and	CC
cord-014597-66vd2mdu	2343	8	Molecule	Molecule	NNP
cord-014597-66vd2mdu	2343	9	Quality	Quality	NNP
cord-014597-66vd2mdu	2343	10	Attributes	Attributes	NNPS
cord-014597-66vd2mdu	2343	11	of	of	IN
cord-014597-66vd2mdu	2343	12	Industrial	Industrial	NNP
cord-014597-66vd2mdu	2343	13	Cell	Cell	NNP
cord-014597-66vd2mdu	2343	14	Lines	line	NNS
cord-014597-66vd2mdu	2343	15	across	across	IN
cord-014597-66vd2mdu	2343	16	Different	different	JJ
cord-014597-66vd2mdu	2343	17	Fed	Fed	NNP
cord-014597-66vd2mdu	2343	18	-	-	HYPH
cord-014597-66vd2mdu	2343	19	Batch	Batch	NNP
cord-014597-66vd2mdu	2343	20	Systems	Systems	NNPS
cord-014597-66vd2mdu	2343	21	Amanullah	Amanullah	NNP
cord-014597-66vd2mdu	2343	22	:	:	:
cord-014597-66vd2mdu	2344	1	Quantitative	quantitative	JJ
cord-014597-66vd2mdu	2344	2	modeling	modeling	NN
cord-014597-66vd2mdu	2344	3	of	of	IN
cord-014597-66vd2mdu	2344	4	viable	viable	JJ
cord-014597-66vd2mdu	2344	5	cell	cell	NN
cord-014597-66vd2mdu	2344	6	density	density	NN
cord-014597-66vd2mdu	2344	7	,	,	,
cord-014597-66vd2mdu	2344	8	cell	cell	NN
cord-014597-66vd2mdu	2344	9	size	size	NN
cord-014597-66vd2mdu	2344	10	,	,	,
cord-014597-66vd2mdu	2344	11	intracellular	intracellular	JJ
cord-014597-66vd2mdu	2344	12	conductivity	conductivity	NN
cord-014597-66vd2mdu	2344	13	,	,	,
cord-014597-66vd2mdu	2344	14	and	and	CC
cord-014597-66vd2mdu	2344	15	membrane	membrane	NN
cord-014597-66vd2mdu	2344	16	capacitance	capacitance	NN
cord-014597-66vd2mdu	2344	17	in	in	IN
cord-014597-66vd2mdu	2344	18	batch	batch	NN
cord-014597-66vd2mdu	2344	19	and	and	CC
cord-014597-66vd2mdu	2344	20	fed	fed	JJ
cord-014597-66vd2mdu	2344	21	-	-	HYPH
cord-014597-66vd2mdu	2344	22	batch	batch	NN
cord-014597-66vd2mdu	2344	23	CHO	CHO	NNP
cord-014597-66vd2mdu	2344	24	processes	process	NNS
cord-014597-66vd2mdu	2344	25	using	use	VBG
cord-014597-66vd2mdu	2344	26	dielectric	dielectric	NN
cord-014597-66vd2mdu	2344	27	spectroscopy	spectroscopy	NN
cord-014597-66vd2mdu	2344	28	Optimal	Optimal	NNP
cord-014597-66vd2mdu	2344	29	and	and	CC
cord-014597-66vd2mdu	2344	30	consistent	consistent	JJ
cord-014597-66vd2mdu	2344	31	protein	protein	NN
cord-014597-66vd2mdu	2344	32	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	2344	33	in	in	IN
cord-014597-66vd2mdu	2344	34	mammcalian	mammcalian	JJ
cord-014597-66vd2mdu	2344	35	cell	cell	NN
cord-014597-66vd2mdu	2344	36	culture	culture	NN
cord-014597-66vd2mdu	2344	37	Journal	Journal	NNP
cord-014597-66vd2mdu	2344	38	of	of	IN
cord-014597-66vd2mdu	2344	39	laboratory	laboratory	NNP
cord-014597-66vd2mdu	2344	40	automation	automation	NN
cord-014597-66vd2mdu	2344	41	.	.	.
cord-014597-66vd2mdu	2345	1	Designs	design	NNS
cord-014597-66vd2mdu	2345	2	and	and	CC
cord-014597-66vd2mdu	2345	3	Concept	Concept	NNP
cord-014597-66vd2mdu	2345	4	-	-	HYPH
cord-014597-66vd2mdu	2345	5	Reliance	Reliance	NNP
cord-014597-66vd2mdu	2345	6	of	of	IN
cord-014597-66vd2mdu	2345	7	a	a	DT
cord-014597-66vd2mdu	2345	8	Fully	fully	RB
cord-014597-66vd2mdu	2345	9	Automated	Automated	NNP
cord-014597-66vd2mdu	2345	10	High	High	NNP
cord-014597-66vd2mdu	2345	11	Content	Content	NNP
cord-014597-66vd2mdu	2345	12	Screening	Screening	NNP
cord-014597-66vd2mdu	2345	13	Platform	Platform	NNP
cord-014597-66vd2mdu	2345	14	Mini	Mini	NNP
cord-014597-66vd2mdu	2345	15	-	-	HYPH
cord-014597-66vd2mdu	2345	16	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2345	17	as	as	IN
cord-014597-66vd2mdu	2345	18	a	a	DT
cord-014597-66vd2mdu	2345	19	highthroughput	highthroughput	NN
cord-014597-66vd2mdu	2345	20	tool	tool	NN
cord-014597-66vd2mdu	2345	21	for	for	IN
cord-014597-66vd2mdu	2345	22	culture	culture	NN
cord-014597-66vd2mdu	2345	23	process	process	NN
cord-014597-66vd2mdu	2345	24	development	development	NN
cord-014597-66vd2mdu	2345	25	to	to	TO
cord-014597-66vd2mdu	2345	26	accelerate	accelerate	VB
cord-014597-66vd2mdu	2345	27	transfer	transfer	NN
cord-014597-66vd2mdu	2345	28	to	to	IN
cord-014597-66vd2mdu	2345	29	stainless	stainless	JJ
cord-014597-66vd2mdu	2345	30	steel	steel	NN
cord-014597-66vd2mdu	2345	31	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	2345	32	scale	scale	NN
cord-014597-66vd2mdu	2345	33	:	:	:
cord-014597-66vd2mdu	2345	34	comparability	comparability	NN
cord-014597-66vd2mdu	2345	35	study	study	NN
cord-014597-66vd2mdu	2345	36	from	from	IN
cord-014597-66vd2mdu	2345	37	process	process	NN
cord-014597-66vd2mdu	2345	38	performance	performance	NN
cord-014597-66vd2mdu	2345	39	to	to	TO
cord-014597-66vd2mdu	2345	40	product	product	NN
cord-014597-66vd2mdu	2345	41	quality	quality	NN
cord-014597-66vd2mdu	2345	42	attributes	attribute	VBZ
cord-014597-66vd2mdu	2345	43	Perfusion	perfusion	NN
cord-014597-66vd2mdu	2345	44	media	medium	NNS
cord-014597-66vd2mdu	2345	45	development	development	NN
cord-014597-66vd2mdu	2345	46	using	use	VBG
cord-014597-66vd2mdu	2345	47	cell	cell	NN
cord-014597-66vd2mdu	2345	48	settling	settle	VBG
cord-014597-66vd2mdu	2345	49	in	in	IN
cord-014597-66vd2mdu	2345	50	automated	automated	JJ
cord-014597-66vd2mdu	2345	51	cell	cell	NN
cord-014597-66vd2mdu	2345	52	culture	culture	NN
cord-014597-66vd2mdu	2345	53	system	system	NN
cord-014597-66vd2mdu	2345	54	Model	model	NN
cord-014597-66vd2mdu	2345	55	-	-	HYPH
cord-014597-66vd2mdu	2345	56	based	base	VBN
cord-014597-66vd2mdu	2345	57	Design	Design	NNP
cord-014597-66vd2mdu	2345	58	of	of	IN
cord-014597-66vd2mdu	2345	59	Process	Process	NNP
cord-014597-66vd2mdu	2345	60	Strategies	Strategies	NNPS
cord-014597-66vd2mdu	2345	61	for	for	IN
cord-014597-66vd2mdu	2345	62	Cell	cell	NN
cord-014597-66vd2mdu	2345	63	Culture	culture	NN
cord-014597-66vd2mdu	2345	64	Bioprocesses	Bioprocesses	NNP
cord-014597-66vd2mdu	2345	65	:	:	:
cord-014597-66vd2mdu	2345	66	State	state	NN
cord-014597-66vd2mdu	2345	67	of	of	IN
cord-014597-66vd2mdu	2345	68	the	the	DT
cord-014597-66vd2mdu	2345	69	Art	Art	NNP
cord-014597-66vd2mdu	2345	70	and	and	CC
cord-014597-66vd2mdu	2345	71	New	New	NNP
cord-014597-66vd2mdu	2345	72	Perspectives	Perspectives	NNPS
cord-014597-66vd2mdu	2345	73	Model	Model	NNP
cord-014597-66vd2mdu	2345	74	-	-	HYPH
cord-014597-66vd2mdu	2345	75	based	base	VBN
cord-014597-66vd2mdu	2345	76	strategy	strategy	NN
cord-014597-66vd2mdu	2345	77	for	for	IN
cord-014597-66vd2mdu	2345	78	cell	cell	NN
cord-014597-66vd2mdu	2345	79	culture	culture	NN
cord-014597-66vd2mdu	2345	80	seed	seed	NN
cord-014597-66vd2mdu	2345	81	train	train	NN
cord-014597-66vd2mdu	2345	82	layout	layout	NN
cord-014597-66vd2mdu	2345	83	verified	verify	VBN
cord-014597-66vd2mdu	2345	84	at	at	IN
cord-014597-66vd2mdu	2345	85	lab	lab	NN
cord-014597-66vd2mdu	2345	86	scale	scale	NN
cord-014597-66vd2mdu	2346	1	Hyperosmotic	hyperosmotic	JJ
cord-014597-66vd2mdu	2346	2	stimulus	stimulus	NN
cord-014597-66vd2mdu	2346	3	study	study	NN
cord-014597-66vd2mdu	2346	4	discloses	disclose	VBZ
cord-014597-66vd2mdu	2346	5	benefits	benefit	NNS
cord-014597-66vd2mdu	2346	6	in	in	IN
cord-014597-66vd2mdu	2346	7	ATP	ATP	NNP
cord-014597-66vd2mdu	2346	8	supply	supply	NN
cord-014597-66vd2mdu	2346	9	and	and	CC
cord-014597-66vd2mdu	2346	10	reveals	reveal	VBZ
cord-014597-66vd2mdu	2346	11	miRNA	miRNA	NNP
cord-014597-66vd2mdu	2346	12	/	/	SYM
cord-014597-66vd2mdu	2346	13	mRNA	mRNA	NNP
cord-014597-66vd2mdu	2346	14	targets	target	VBZ
cord-014597-66vd2mdu	2346	15	to	to	TO
cord-014597-66vd2mdu	2346	16	improve	improve	VB
cord-014597-66vd2mdu	2346	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2346	18	protein	protein	NN
cord-014597-66vd2mdu	2346	19	production	production	NN
cord-014597-66vd2mdu	2346	20	of	of	IN
cord-014597-66vd2mdu	2346	21	CHO	CHO	NNP
cord-014597-66vd2mdu	2346	22	cells	cell	NNS
cord-014597-66vd2mdu	2346	23	Effects	effect	NNS
cord-014597-66vd2mdu	2346	24	of	of	IN
cord-014597-66vd2mdu	2346	25	osmoprotectant	osmoprotectant	JJ
cord-014597-66vd2mdu	2346	26	compounds	compound	NNS
cord-014597-66vd2mdu	2346	27	on	on	IN
cord-014597-66vd2mdu	2346	28	NCAM	NCAM	NNP
cord-014597-66vd2mdu	2346	29	polysialylation	polysialylation	NN
cord-014597-66vd2mdu	2346	30	under	under	IN
cord-014597-66vd2mdu	2346	31	hyperosmotic	hyperosmotic	JJ
cord-014597-66vd2mdu	2346	32	stress	stress	NN
cord-014597-66vd2mdu	2346	33	and	and	CC
cord-014597-66vd2mdu	2346	34	elevated	elevate	VBN
cord-014597-66vd2mdu	2346	35	pCO	pco	CD
cord-014597-66vd2mdu	2346	36	2	2	CD
cord-014597-66vd2mdu	2346	37	Evaluating	evaluate	VBG
cord-014597-66vd2mdu	2346	38	the	the	DT
cord-014597-66vd2mdu	2346	39	bottlenecks	bottleneck	NNS
cord-014597-66vd2mdu	2346	40	of	of	IN
cord-014597-66vd2mdu	2346	41	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2346	42	IgM	IgM	NNP
cord-014597-66vd2mdu	2346	43	production	production	NN
cord-014597-66vd2mdu	2346	44	in	in	IN
cord-014597-66vd2mdu	2346	45	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2346	46	cells	cell	NNS
cord-014597-66vd2mdu	2346	47	IgM	IgM	NNP
cord-014597-66vd2mdu	2346	48	characterization	characterization	NN
cord-014597-66vd2mdu	2346	49	directly	directly	RB
cord-014597-66vd2mdu	2346	50	performed	perform	VBN
cord-014597-66vd2mdu	2346	51	in	in	IN
cord-014597-66vd2mdu	2346	52	crude	crude	JJ
cord-014597-66vd2mdu	2346	53	culture	culture	NN
cord-014597-66vd2mdu	2346	54	supernatants	supernatant	NNS
cord-014597-66vd2mdu	2346	55	by	by	IN
cord-014597-66vd2mdu	2346	56	a	a	DT
cord-014597-66vd2mdu	2346	57	new	new	JJ
cord-014597-66vd2mdu	2346	58	simple	simple	JJ
cord-014597-66vd2mdu	2346	59	electrophoretic	electrophoretic	JJ
cord-014597-66vd2mdu	2346	60	method	method	NN
cord-014597-66vd2mdu	2346	61	Effect	effect	NN
cord-014597-66vd2mdu	2346	62	of	of	IN
cord-014597-66vd2mdu	2346	63	culture	culture	NN
cord-014597-66vd2mdu	2347	1	pH	pH	NNP
cord-014597-66vd2mdu	2347	2	on	on	IN
cord-014597-66vd2mdu	2347	3	erythropoietin	erythropoietin	NN
cord-014597-66vd2mdu	2347	4	production	production	NN
cord-014597-66vd2mdu	2347	5	by	by	IN
cord-014597-66vd2mdu	2347	6	Chinese	chinese	JJ
cord-014597-66vd2mdu	2347	7	hamster	hamster	NN
cord-014597-66vd2mdu	2347	8	ovary	ovary	NN
cord-014597-66vd2mdu	2347	9	cells	cell	NNS
cord-014597-66vd2mdu	2347	10	grown	grow	VBN
cord-014597-66vd2mdu	2347	11	in	in	IN
cord-014597-66vd2mdu	2347	12	suspension	suspension	NN
cord-014597-66vd2mdu	2347	13	at	at	IN
cord-014597-66vd2mdu	2347	14	32.5	32.5	CD
cord-014597-66vd2mdu	2347	15	and	and	CC
cord-014597-66vd2mdu	2347	16	37.0	37.0	CD
cord-014597-66vd2mdu	2347	17	°	°	NN
cord-014597-66vd2mdu	2347	18	C	C	NNP
cord-014597-66vd2mdu	2347	19	Enhancing	Enhancing	NNP
cord-014597-66vd2mdu	2347	20	Protein	Protein	NNP
cord-014597-66vd2mdu	2347	21	Expression	Expression	NNP
cord-014597-66vd2mdu	2347	22	in	in	IN
cord-014597-66vd2mdu	2347	23	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	2347	24	Cells	cell	NNS
cord-014597-66vd2mdu	2347	25	by	by	IN
cord-014597-66vd2mdu	2347	26	Lowering	lower	VBG
cord-014597-66vd2mdu	2347	27	Culture	culture	NN
cord-014597-66vd2mdu	2347	28	Temperature	temperature	NN
cord-014597-66vd2mdu	2347	29	Relationship	relationship	NN
cord-014597-66vd2mdu	2347	30	between	between	IN
cord-014597-66vd2mdu	2347	31	tissue	tissue	NN
cord-014597-66vd2mdu	2347	32	plasminogen	plasminogen	NN
cord-014597-66vd2mdu	2347	33	activator	activator	NN
cord-014597-66vd2mdu	2347	34	production	production	NN
cord-014597-66vd2mdu	2347	35	and	and	CC
cord-014597-66vd2mdu	2347	36	specific	specific	JJ
cord-014597-66vd2mdu	2347	37	growth	growth	NN
cord-014597-66vd2mdu	2347	38	rate	rate	NN
cord-014597-66vd2mdu	2347	39	in	in	IN
cord-014597-66vd2mdu	2347	40	Chinese	chinese	JJ
cord-014597-66vd2mdu	2347	41	Hamster	Hamster	NNP
cord-014597-66vd2mdu	2347	42	Ovary	Ovary	NNP
cord-014597-66vd2mdu	2347	43	cells	cell	NNS
cord-014597-66vd2mdu	2347	44	cultured	culture	VBN
cord-014597-66vd2mdu	2347	45	in	in	IN
cord-014597-66vd2mdu	2347	46	mannose	mannose	NN
cord-014597-66vd2mdu	2347	47	at	at	IN
cord-014597-66vd2mdu	2347	48	low	low	JJ
cord-014597-66vd2mdu	2347	49	temperature	temperature	NN
cord-014597-66vd2mdu	2348	1	A	a	DT
cord-014597-66vd2mdu	2348	2	quantitative	quantitative	JJ
cord-014597-66vd2mdu	2348	3	proteomic	proteomic	JJ
cord-014597-66vd2mdu	2348	4	analysis	analysis	NN
cord-014597-66vd2mdu	2348	5	of	of	IN
cord-014597-66vd2mdu	2348	6	cellular	cellular	JJ
cord-014597-66vd2mdu	2348	7	responses	response	NNS
cord-014597-66vd2mdu	2348	8	to	to	IN
cord-014597-66vd2mdu	2348	9	high	high	JJ
cord-014597-66vd2mdu	2348	10	glucose	glucose	NN
cord-014597-66vd2mdu	2348	11	media	medium	NNS
cord-014597-66vd2mdu	2348	12	in	in	IN
cord-014597-66vd2mdu	2348	13	Chinese	chinese	JJ
cord-014597-66vd2mdu	2348	14	hamster	hamster	NN
cord-014597-66vd2mdu	2348	15	ovary	ovary	NN
cord-014597-66vd2mdu	2348	16	cells	cell	NNS
cord-014597-66vd2mdu	2349	1	Process	process	NN
cord-014597-66vd2mdu	2349	2	analytical	analytical	JJ
cord-014597-66vd2mdu	2349	3	technologies	technology	NNS
cord-014597-66vd2mdu	2349	4	in	in	IN
cord-014597-66vd2mdu	2349	5	the	the	DT
cord-014597-66vd2mdu	2349	6	pharmaceutical	pharmaceutical	NN
cord-014597-66vd2mdu	2349	7	industry	industry	NN
cord-014597-66vd2mdu	2349	8	:	:	:
cord-014597-66vd2mdu	2349	9	the	the	DT
cord-014597-66vd2mdu	2349	10	FDA	FDA	NNP
cord-014597-66vd2mdu	2349	11	's	's	POS
cord-014597-66vd2mdu	2349	12	PAT	PAT	NNP
cord-014597-66vd2mdu	2349	13	initiative	initiative	NN
cord-014597-66vd2mdu	2349	14	Effects	effect	NNS
cord-014597-66vd2mdu	2349	15	of	of	IN
cord-014597-66vd2mdu	2349	16	ammonium	ammonium	NN
cord-014597-66vd2mdu	2349	17	and	and	CC
cord-014597-66vd2mdu	2349	18	lactate	lactate	NN
cord-014597-66vd2mdu	2349	19	on	on	IN
cord-014597-66vd2mdu	2349	20	growth	growth	NN
cord-014597-66vd2mdu	2349	21	and	and	CC
cord-014597-66vd2mdu	2349	22	metabolism	metabolism	NN
cord-014597-66vd2mdu	2349	23	of	of	IN
cord-014597-66vd2mdu	2349	24	a	a	DT
cord-014597-66vd2mdu	2349	25	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2349	26	Chinese	chinese	JJ
cord-014597-66vd2mdu	2349	27	hamster	hamster	NN
cord-014597-66vd2mdu	2349	28	ovary	ovary	NN
cord-014597-66vd2mdu	2349	29	cell	cell	NN
cord-014597-66vd2mdu	2349	30	culture	culture	NN
cord-014597-66vd2mdu	2349	31	Glycosylation	Glycosylation	NNP
cord-014597-66vd2mdu	2349	32	in	in	IN
cord-014597-66vd2mdu	2349	33	Cell	Cell	NNP
cord-014597-66vd2mdu	2349	34	Culture	Culture	NNP
cord-014597-66vd2mdu	2349	35	Structural	structural	JJ
cord-014597-66vd2mdu	2349	36	mechanism	mechanism	NN
cord-014597-66vd2mdu	2349	37	of	of	IN
cord-014597-66vd2mdu	2349	38	high	high	JJ
cord-014597-66vd2mdu	2349	39	affinity	affinity	NN
cord-014597-66vd2mdu	2349	40	FcgammaRI	FcgammaRI	NNP
cord-014597-66vd2mdu	2349	41	recognition	recognition	NN
cord-014597-66vd2mdu	2349	42	of	of	IN
cord-014597-66vd2mdu	2349	43	immunoglobulin	immunoglobulin	NN
cord-014597-66vd2mdu	2349	44	G	G	NNP
cord-014597-66vd2mdu	2349	45	Inhibition	inhibition	NN
cord-014597-66vd2mdu	2349	46	of	of	IN
cord-014597-66vd2mdu	2349	47	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	2349	48	on	on	IN
cord-014597-66vd2mdu	2349	49	a	a	DT
cord-014597-66vd2mdu	2349	50	camelid	camelid	NN
cord-014597-66vd2mdu	2349	51	antibody	antibody	NN
cord-014597-66vd2mdu	2349	52	uniquely	uniquely	RB
cord-014597-66vd2mdu	2349	53	affects	affect	VBZ
cord-014597-66vd2mdu	2349	54	its	-PRON-	PRP$
cord-014597-66vd2mdu	2349	55	FcγRI	FcγRI	NNP
cord-014597-66vd2mdu	2349	56	binding	bind	VBG
cord-014597-66vd2mdu	2349	57	activity	activity	NN
cord-014597-66vd2mdu	2349	58	Investigation	Investigation	NNP
cord-014597-66vd2mdu	2349	59	of	of	IN
cord-014597-66vd2mdu	2349	60	CHO	CHO	NNP
cord-014597-66vd2mdu	2349	61	Secretome	Secretome	NNP
cord-014597-66vd2mdu	2349	62	:	:	:
cord-014597-66vd2mdu	2349	63	Potential	potential	JJ
cord-014597-66vd2mdu	2349	64	Way	way	NN
cord-014597-66vd2mdu	2349	65	to	to	TO
cord-014597-66vd2mdu	2349	66	Improve	improve	VB
cord-014597-66vd2mdu	2349	67	Recombinant	Recombinant	NNP
cord-014597-66vd2mdu	2349	68	Protein	Protein	NNP
cord-014597-66vd2mdu	2349	69	Production	Production	NNP
cord-014597-66vd2mdu	2349	70	from	from	IN
cord-014597-66vd2mdu	2349	71	Bioprocess	Bioprocess	NNP
cord-014597-66vd2mdu	2349	72	Bladder	Bladder	NNP
cord-014597-66vd2mdu	2349	73	cancer	cancer	NN
cord-014597-66vd2mdu	2350	1	cell	cell	NN
cord-014597-66vd2mdu	2350	2	-	-	HYPH
cord-014597-66vd2mdu	2350	3	derived	derive	VBN
cord-014597-66vd2mdu	2350	4	exosomes	exosome	NNS
cord-014597-66vd2mdu	2350	5	inhibit	inhibit	NN
cord-014597-66vd2mdu	2350	6	tumor	tumor	NN
cord-014597-66vd2mdu	2350	7	cell	cell	NN
cord-014597-66vd2mdu	2350	8	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	2350	9	and	and	CC
cord-014597-66vd2mdu	2350	10	induce	induce	VB
cord-014597-66vd2mdu	2350	11	cell	cell	NN
cord-014597-66vd2mdu	2350	12	proliferation	proliferation	NN
cord-014597-66vd2mdu	2350	13	in	in	IN
cord-014597-66vd2mdu	2350	14	vitro	vitro	FW
cord-014597-66vd2mdu	2350	15	Exploring	explore	VBG
cord-014597-66vd2mdu	2350	16	Packaged	package	VBN
cord-014597-66vd2mdu	2350	17	Microvesicle	Microvesicle	NNP
cord-014597-66vd2mdu	2350	18	Proteome	Proteome	NNP
cord-014597-66vd2mdu	2350	19	Composition	Composition	NNP
cord-014597-66vd2mdu	2350	20	of	of	IN
cord-014597-66vd2mdu	2350	21	Chinese	chinese	JJ
cord-014597-66vd2mdu	2350	22	Hamster	Hamster	NNP
cord-014597-66vd2mdu	2350	23	Ovary	Ovary	NNP
cord-014597-66vd2mdu	2350	24	Secretome	Secretome	NNP
cord-014597-66vd2mdu	2350	25	Glycome	Glycome	NNP
cord-014597-66vd2mdu	2350	26	mapping	mapping	NN
cord-014597-66vd2mdu	2350	27	on	on	IN
cord-014597-66vd2mdu	2350	28	DNA	dna	NN
cord-014597-66vd2mdu	2350	29	sequencing	sequencing	NN
cord-014597-66vd2mdu	2350	30	equipment	equipment	NN
cord-014597-66vd2mdu	2350	31	Iron	iron	NN
cord-014597-66vd2mdu	2350	32	(	(	-LRB-
cord-014597-66vd2mdu	2350	33	III	III	NNP
cord-014597-66vd2mdu	2350	34	)	)	-RRB-
cord-014597-66vd2mdu	2351	1	citrate	citrate	NNP
cord-014597-66vd2mdu	2351	2	inhibits	inhibit	VBZ
cord-014597-66vd2mdu	2351	3	polyethylenimine	polyethylenimine	NNP
cord-014597-66vd2mdu	2351	4	-	-	HYPH
cord-014597-66vd2mdu	2351	5	mediated	mediate	VBN
cord-014597-66vd2mdu	2351	6	transient	transient	JJ
cord-014597-66vd2mdu	2351	7	transfection	transfection	NN
cord-014597-66vd2mdu	2351	8	of	of	IN
cord-014597-66vd2mdu	2351	9	Chinese	chinese	JJ
cord-014597-66vd2mdu	2351	10	hamster	hamster	NN
cord-014597-66vd2mdu	2351	11	ovary	ovary	NN
cord-014597-66vd2mdu	2351	12	cells	cell	NNS
cord-014597-66vd2mdu	2351	13	in	in	IN
cord-014597-66vd2mdu	2351	14	serum	serum	NN
cord-014597-66vd2mdu	2351	15	-	-	HYPH
cord-014597-66vd2mdu	2351	16	free	free	JJ
cord-014597-66vd2mdu	2351	17	medium	medium	NN
cord-014597-66vd2mdu	2351	18	Efficient	efficient	JJ
cord-014597-66vd2mdu	2351	19	high	high	JJ
cord-014597-66vd2mdu	2351	20	-	-	HYPH
cord-014597-66vd2mdu	2351	21	throughput	throughput	NN
cord-014597-66vd2mdu	2351	22	biological	biological	JJ
cord-014597-66vd2mdu	2351	23	process	process	NN
cord-014597-66vd2mdu	2351	24	characterization	characterization	NN
cord-014597-66vd2mdu	2351	25	Scale	scale	NN
cord-014597-66vd2mdu	2351	26	-	-	HYPH
cord-014597-66vd2mdu	2351	27	up	up	NN
cord-014597-66vd2mdu	2351	28	of	of	IN
cord-014597-66vd2mdu	2351	29	a	a	DT
cord-014597-66vd2mdu	2351	30	stirred	stir	VBN
cord-014597-66vd2mdu	2351	31	single	single	JJ
cord-014597-66vd2mdu	2351	32	-	-	HYPH
cord-014597-66vd2mdu	2351	33	use	use	NN
cord-014597-66vd2mdu	2351	34	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2351	35	family	family	NN
cord-014597-66vd2mdu	2351	36	Quality	quality	NN
cord-014597-66vd2mdu	2351	37	control	control	NN
cord-014597-66vd2mdu	2351	38	:	:	:
cord-014597-66vd2mdu	2351	39	ER	er	NN
cord-014597-66vd2mdu	2351	40	-	-	HYPH
cord-014597-66vd2mdu	2351	41	associated	associate	VBN
cord-014597-66vd2mdu	2351	42	degradation	degradation	NN
cord-014597-66vd2mdu	2351	43	:	:	:
cord-014597-66vd2mdu	2351	44	protein	protein	NN
cord-014597-66vd2mdu	2351	45	quality	quality	NN
cord-014597-66vd2mdu	2351	46	control	control	NN
cord-014597-66vd2mdu	2351	47	and	and	CC
cord-014597-66vd2mdu	2351	48	beyond	beyond	IN
cord-014597-66vd2mdu	2352	1	Pharmacological	pharmacological	JJ
cord-014597-66vd2mdu	2352	2	targeting	targeting	NN
cord-014597-66vd2mdu	2352	3	of	of	IN
cord-014597-66vd2mdu	2352	4	endoplasmic	endoplasmic	NN
cord-014597-66vd2mdu	2352	5	reticulum	reticulum	NN
cord-014597-66vd2mdu	2352	6	stress	stress	NN
cord-014597-66vd2mdu	2352	7	signaling	signal	VBG
cord-014597-66vd2mdu	2352	8	in	in	IN
cord-014597-66vd2mdu	2352	9	cancer	cancer	NN
cord-014597-66vd2mdu	2352	10	When	when	WRB
cord-014597-66vd2mdu	2352	11	ER	ER	NNP
cord-014597-66vd2mdu	2352	12	stress	stress	NN
cord-014597-66vd2mdu	2352	13	reaches	reach	VBZ
cord-014597-66vd2mdu	2352	14	a	a	DT
cord-014597-66vd2mdu	2352	15	dead	dead	JJ
cord-014597-66vd2mdu	2352	16	end	end	NN
cord-014597-66vd2mdu	2352	17	Oxidative	oxidative	JJ
cord-014597-66vd2mdu	2352	18	stress	stress	NN
cord-014597-66vd2mdu	2352	19	and	and	CC
cord-014597-66vd2mdu	2352	20	antioxidant	antioxidant	NN
cord-014597-66vd2mdu	2352	21	defense	defense	NN
cord-014597-66vd2mdu	2353	1	The	the	DT
cord-014597-66vd2mdu	2353	2	antioxidant	antioxidant	JJ
cord-014597-66vd2mdu	2353	3	edaravone	edaravone	NN
cord-014597-66vd2mdu	2353	4	attenuates	attenuate	VBZ
cord-014597-66vd2mdu	2353	5	ER	er	NN
cord-014597-66vd2mdu	2353	6	-	-	HYPH
cord-014597-66vd2mdu	2353	7	stress	stress	NN
cord-014597-66vd2mdu	2353	8	-	-	HYPH
cord-014597-66vd2mdu	2353	9	mediated	mediate	VBN
cord-014597-66vd2mdu	2353	10	cardiac	cardiac	JJ
cord-014597-66vd2mdu	2353	11	apoptosis	apoptosis	NN
cord-014597-66vd2mdu	2353	12	and	and	CC
cord-014597-66vd2mdu	2353	13	dysfunction	dysfunction	NN
cord-014597-66vd2mdu	2353	14	in	in	IN
cord-014597-66vd2mdu	2353	15	rats	rat	NNS
cord-014597-66vd2mdu	2353	16	with	with	IN
cord-014597-66vd2mdu	2353	17	autoimmune	autoimmune	JJ
cord-014597-66vd2mdu	2353	18	myocarditis	myocarditis	NN
cord-014597-66vd2mdu	2353	19	Advanced	Advanced	NNP
cord-014597-66vd2mdu	2353	20	Process	Process	NNP
cord-014597-66vd2mdu	2353	21	and	and	CC
cord-014597-66vd2mdu	2353	22	Control	Control	NNP
cord-014597-66vd2mdu	2353	23	Strategies	Strategies	NNPS
cord-014597-66vd2mdu	2353	24	for	for	IN
cord-014597-66vd2mdu	2353	25	Bioreactors	bioreactor	NNS
cord-014597-66vd2mdu	2353	26	.	.	.
cord-014597-66vd2mdu	2354	1	Book	Book	NNP
cord-014597-66vd2mdu	2354	2	chapter	chapter	NN
cord-014597-66vd2mdu	2354	3	Model	Model	NNP
cord-014597-66vd2mdu	2354	4	-	-	HYPH
cord-014597-66vd2mdu	2354	5	based	base	VBN
cord-014597-66vd2mdu	2354	6	strategy	strategy	NN
cord-014597-66vd2mdu	2354	7	for	for	IN
cord-014597-66vd2mdu	2354	8	cell	cell	NN
cord-014597-66vd2mdu	2354	9	culture	culture	NN
cord-014597-66vd2mdu	2354	10	seed	seed	NN
cord-014597-66vd2mdu	2354	11	train	train	NN
cord-014597-66vd2mdu	2354	12	layout	layout	NN
cord-014597-66vd2mdu	2354	13	verified	verify	VBN
cord-014597-66vd2mdu	2354	14	at	at	IN
cord-014597-66vd2mdu	2354	15	lab	lab	NN
cord-014597-66vd2mdu	2354	16	scale	scale	NN
cord-014597-66vd2mdu	2354	17	Seed	seed	NN
cord-014597-66vd2mdu	2354	18	train	train	NN
cord-014597-66vd2mdu	2354	19	optimization	optimization	NN
cord-014597-66vd2mdu	2354	20	for	for	IN
cord-014597-66vd2mdu	2354	21	cell	cell	NN
cord-014597-66vd2mdu	2354	22	culture	culture	NN
cord-014597-66vd2mdu	2354	23	.	.	.
cord-014597-66vd2mdu	2355	1	Chapter	chapter	NN
cord-014597-66vd2mdu	2355	2	High	high	JJ
cord-014597-66vd2mdu	2355	3	Cell	Cell	NNP
cord-014597-66vd2mdu	2355	4	Density	density	NN
cord-014597-66vd2mdu	2355	5	Cultivation	Cultivation	NNP
cord-014597-66vd2mdu	2355	6	of	of	IN
cord-014597-66vd2mdu	2355	7	Human	Human	NNP
cord-014597-66vd2mdu	2355	8	Leukemia	Leukemia	NNP
cord-014597-66vd2mdu	2355	9	T	T	NNP
cord-014597-66vd2mdu	2355	10	Cells	cell	NNS
cord-014597-66vd2mdu	2355	11	(	(	-LRB-
cord-014597-66vd2mdu	2355	12	Jurkat	Jurkat	NNP
cord-014597-66vd2mdu	2355	13	Cells	Cells	NNP
cord-014597-66vd2mdu	2355	14	)	)	-RRB-
cord-014597-66vd2mdu	2355	15	in	in	IN
cord-014597-66vd2mdu	2355	16	Semipermeable	Semipermeable	NNP
cord-014597-66vd2mdu	2355	17	Polyelectrolyte	Polyelectrolyte	NNP
cord-014597-66vd2mdu	2355	18	Microcapsules	Microcapsules	NNP
cord-014597-66vd2mdu	2355	19	.	.	.
cord-014597-66vd2mdu	2356	1	Eng	Eng	NNP
cord-014597-66vd2mdu	2356	2	.	.	.
cord-014597-66vd2mdu	2357	1	Life	Life	NNP
cord-014597-66vd2mdu	2357	2	Sci	Sci	NNP
cord-014597-66vd2mdu	2357	3	Cell	Cell	NNP
cord-014597-66vd2mdu	2357	4	retention	retention	NN
cord-014597-66vd2mdu	2357	5	by	by	IN
cord-014597-66vd2mdu	2357	6	encapsulation	encapsulation	NN
cord-014597-66vd2mdu	2357	7	for	for	IN
cord-014597-66vd2mdu	2357	8	the	the	DT
cord-014597-66vd2mdu	2357	9	cultivation	cultivation	NN
cord-014597-66vd2mdu	2357	10	of	of	IN
cord-014597-66vd2mdu	2357	11	Jurkat	Jurkat	NNP
cord-014597-66vd2mdu	2357	12	cells	cell	NNS
cord-014597-66vd2mdu	2357	13	in	in	IN
cord-014597-66vd2mdu	2357	14	fixed	fix	VBN
cord-014597-66vd2mdu	2357	15	and	and	CC
cord-014597-66vd2mdu	2357	16	fluidized	fluidized	JJ
cord-014597-66vd2mdu	2357	17	bed	bed	NN
cord-014597-66vd2mdu	2357	18	reactors	reactor	NNS
cord-014597-66vd2mdu	2358	1	The	the	DT
cord-014597-66vd2mdu	2358	2	role	role	NN
cord-014597-66vd2mdu	2358	3	of	of	IN
cord-014597-66vd2mdu	2358	4	interleukin-2	interleukin-2	NN
cord-014597-66vd2mdu	2358	5	during	during	IN
cord-014597-66vd2mdu	2358	6	homeostasis	homeostasis	NN
cord-014597-66vd2mdu	2358	7	and	and	CC
cord-014597-66vd2mdu	2358	8	activation	activation	NN
cord-014597-66vd2mdu	2358	9	of	of	IN
cord-014597-66vd2mdu	2358	10	the	the	DT
cord-014597-66vd2mdu	2358	11	immune	immune	JJ
cord-014597-66vd2mdu	2358	12	system	system	NN
cord-014597-66vd2mdu	2358	13	Creating	create	VBG
cord-014597-66vd2mdu	2358	14	a	a	DT
cord-014597-66vd2mdu	2358	15	Biomimetic	Biomimetic	NNP
cord-014597-66vd2mdu	2358	16	Microenvironment	Microenvironment	NNP
cord-014597-66vd2mdu	2358	17	for	for	IN
cord-014597-66vd2mdu	2358	18	the	the	DT
cord-014597-66vd2mdu	2358	19	Ex	Ex	NNP
cord-014597-66vd2mdu	2358	20	Vivo	Vivo	NNP
cord-014597-66vd2mdu	2358	21	Expansion	Expansion	NNP
cord-014597-66vd2mdu	2358	22	of	of	IN
cord-014597-66vd2mdu	2358	23	Primary	Primary	NNP
cord-014597-66vd2mdu	2358	24	Human	Human	NNP
cord-014597-66vd2mdu	2359	1	The	the	DT
cord-014597-66vd2mdu	2359	2	Era	Era	NNP
cord-014597-66vd2mdu	2359	3	of	of	IN
cord-014597-66vd2mdu	2359	4	Digital	Digital	NNP
cord-014597-66vd2mdu	2359	5	Biomanufacturing	Biomanufacturing	NNP
cord-014597-66vd2mdu	2360	1	The	the	DT
cord-014597-66vd2mdu	2360	2	Multivariate	Multivariate	NNP
cord-014597-66vd2mdu	2360	3	Normal	normal	JJ
cord-014597-66vd2mdu	2360	4	Distribution	distribution	NN
cord-014597-66vd2mdu	2360	5	Recovery	Recovery	NNP
cord-014597-66vd2mdu	2360	6	from	from	IN
cord-014597-66vd2mdu	2360	7	rabies	rabies	NN
cord-014597-66vd2mdu	2360	8	:	:	:
cord-014597-66vd2mdu	2361	1	A	a	DT
cord-014597-66vd2mdu	2361	2	call	call	NN
cord-014597-66vd2mdu	2361	3	to	to	IN
cord-014597-66vd2mdu	2361	4	arms	arm	NNS
cord-014597-66vd2mdu	2362	1	The	the	DT
cord-014597-66vd2mdu	2362	2	role	role	NN
cord-014597-66vd2mdu	2362	3	of	of	IN
cord-014597-66vd2mdu	2362	4	vaccination	vaccination	NN
cord-014597-66vd2mdu	2362	5	in	in	IN
cord-014597-66vd2mdu	2362	6	rabies	rabies	NN
cord-014597-66vd2mdu	2362	7	prevention	prevention	NN
cord-014597-66vd2mdu	2363	1	Eliminating	eliminate	VBG
cord-014597-66vd2mdu	2363	2	canine	canine	JJ
cord-014597-66vd2mdu	2363	3	rabies	rabie	NNS
cord-014597-66vd2mdu	2363	4	,	,	,
cord-014597-66vd2mdu	2363	5	the	the	DT
cord-014597-66vd2mdu	2363	6	principal	principal	JJ
cord-014597-66vd2mdu	2363	7	source	source	NN
cord-014597-66vd2mdu	2363	8	of	of	IN
cord-014597-66vd2mdu	2363	9	human	human	JJ
cord-014597-66vd2mdu	2363	10	infection	infection	NN
cord-014597-66vd2mdu	2363	11	:	:	:
cord-014597-66vd2mdu	2363	12	What	what	WP
cord-014597-66vd2mdu	2363	13	will	will	MD
cord-014597-66vd2mdu	2363	14	it	-PRON-	PRP
cord-014597-66vd2mdu	2363	15	take	take	VB
cord-014597-66vd2mdu	2363	16	?	?	.
cord-014597-66vd2mdu	2364	1	Rabies	rabies	NN
cord-014597-66vd2mdu	2364	2	virus	virus	NN
cord-014597-66vd2mdu	2364	3	-	-	HYPH
cord-014597-66vd2mdu	2364	4	like	like	JJ
cord-014597-66vd2mdu	2364	5	particles	particle	NNS
cord-014597-66vd2mdu	2364	6	expressed	express	VBN
cord-014597-66vd2mdu	2364	7	in	in	IN
cord-014597-66vd2mdu	2364	8	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2364	9	cells	cell	NNS
cord-014597-66vd2mdu	2364	10	Immunogenic	immunogenic	JJ
cord-014597-66vd2mdu	2364	11	virus	virus	NN
cord-014597-66vd2mdu	2364	12	-	-	HYPH
cord-014597-66vd2mdu	2364	13	like	like	JJ
cord-014597-66vd2mdu	2364	14	particles	particle	NNS
cord-014597-66vd2mdu	2364	15	continuously	continuously	RB
cord-014597-66vd2mdu	2364	16	expressed	express	VBN
cord-014597-66vd2mdu	2364	17	in	in	IN
cord-014597-66vd2mdu	2364	18	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2364	19	cells	cell	NNS
cord-014597-66vd2mdu	2364	20	as	as	IN
cord-014597-66vd2mdu	2364	21	a	a	DT
cord-014597-66vd2mdu	2364	22	veterinary	veterinary	JJ
cord-014597-66vd2mdu	2364	23	rabies	rabies	NN
cord-014597-66vd2mdu	2364	24	vaccine	vaccine	NN
cord-014597-66vd2mdu	2364	25	candidate	candidate	NN
cord-014597-66vd2mdu	2364	26	An	an	DT
cord-014597-66vd2mdu	2364	27	avian	avian	JJ
cord-014597-66vd2mdu	2364	28	cell	cell	NN
cord-014597-66vd2mdu	2364	29	line	line	NN
cord-014597-66vd2mdu	2364	30	designed	design	VBN
cord-014597-66vd2mdu	2364	31	for	for	IN
cord-014597-66vd2mdu	2364	32	production	production	NN
cord-014597-66vd2mdu	2364	33	of	of	IN
cord-014597-66vd2mdu	2364	34	highly	highly	RB
cord-014597-66vd2mdu	2364	35	attenuated	attenuated	JJ
cord-014597-66vd2mdu	2364	36	viruses	virus	NNS
cord-014597-66vd2mdu	2364	37	A	a	DT
cord-014597-66vd2mdu	2364	38	chemically	chemically	RB
cord-014597-66vd2mdu	2364	39	defined	define	VBN
cord-014597-66vd2mdu	2364	40	production	production	NN
cord-014597-66vd2mdu	2364	41	process	process	NN
cord-014597-66vd2mdu	2364	42	for	for	IN
cord-014597-66vd2mdu	2364	43	highly	highly	RB
cord-014597-66vd2mdu	2364	44	attenuated	attenuate	VBN
cord-014597-66vd2mdu	2364	45	poxviruses	poxvirus	NNS
cord-014597-66vd2mdu	2365	1	A	a	DT
cord-014597-66vd2mdu	2365	2	genotype	genotype	NN
cord-014597-66vd2mdu	2365	3	of	of	IN
cord-014597-66vd2mdu	2365	4	modified	modify	VBN
cord-014597-66vd2mdu	2365	5	vaccinia	vaccinia	NN
cord-014597-66vd2mdu	2365	6	Ankara	Ankara	NNP
cord-014597-66vd2mdu	2365	7	(	(	-LRB-
cord-014597-66vd2mdu	2365	8	MVA	MVA	NNP
cord-014597-66vd2mdu	2365	9	)	)	-RRB-
cord-014597-66vd2mdu	2365	10	that	that	WDT
cord-014597-66vd2mdu	2365	11	facilitates	facilitate	VBZ
cord-014597-66vd2mdu	2365	12	replication	replication	NN
cord-014597-66vd2mdu	2365	13	in	in	IN
cord-014597-66vd2mdu	2365	14	suspension	suspension	NN
cord-014597-66vd2mdu	2365	15	cultures	culture	NNS
cord-014597-66vd2mdu	2365	16	in	in	IN
cord-014597-66vd2mdu	2365	17	chemically	chemically	RB
cord-014597-66vd2mdu	2365	18	defined	define	VBN
cord-014597-66vd2mdu	2365	19	medium	medium	NN
cord-014597-66vd2mdu	2365	20	Easy	easy	JJ
cord-014597-66vd2mdu	2365	21	and	and	CC
cord-014597-66vd2mdu	2365	22	efficient	efficient	JJ
cord-014597-66vd2mdu	2365	23	protocols	protocol	NNS
cord-014597-66vd2mdu	2365	24	for	for	IN
cord-014597-66vd2mdu	2365	25	working	work	VBG
cord-014597-66vd2mdu	2365	26	with	with	IN
cord-014597-66vd2mdu	2365	27	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2365	28	vaccinia	vaccinia	NN
cord-014597-66vd2mdu	2365	29	virus	virus	NN
cord-014597-66vd2mdu	2365	30	MVA	MVA	NNP
cord-014597-66vd2mdu	2365	31	Large	large	JJ
cord-014597-66vd2mdu	2365	32	-	-	HYPH
cord-014597-66vd2mdu	2365	33	scale	scale	NN
cord-014597-66vd2mdu	2365	34	transfection	transfection	NN
cord-014597-66vd2mdu	2365	35	of	of	IN
cord-014597-66vd2mdu	2365	36	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2365	37	cells	cell	NNS
cord-014597-66vd2mdu	2365	38	for	for	IN
cord-014597-66vd2mdu	2365	39	the	the	DT
cord-014597-66vd2mdu	2365	40	fast	fast	JJ
cord-014597-66vd2mdu	2365	41	production	production	NN
cord-014597-66vd2mdu	2365	42	of	of	IN
cord-014597-66vd2mdu	2365	43	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2365	44	protein	protein	NN
cord-014597-66vd2mdu	2365	45	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	2365	46	protein	protein	NN
cord-014597-66vd2mdu	2365	47	production	production	NN
cord-014597-66vd2mdu	2365	48	by	by	IN
cord-014597-66vd2mdu	2365	49	large	large	JJ
cord-014597-66vd2mdu	2365	50	-	-	HYPH
cord-014597-66vd2mdu	2365	51	scale	scale	NN
cord-014597-66vd2mdu	2365	52	transient	transient	JJ
cord-014597-66vd2mdu	2365	53	gene	gene	NN
cord-014597-66vd2mdu	2365	54	expression	expression	NN
cord-014597-66vd2mdu	2365	55	in	in	IN
cord-014597-66vd2mdu	2365	56	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2365	57	cells	cell	NNS
cord-014597-66vd2mdu	2365	58	:	:	:
cord-014597-66vd2mdu	2365	59	state	state	NN
cord-014597-66vd2mdu	2365	60	of	of	IN
cord-014597-66vd2mdu	2365	61	the	the	DT
cord-014597-66vd2mdu	2365	62	art	art	NN
cord-014597-66vd2mdu	2365	63	and	and	CC
cord-014597-66vd2mdu	2365	64	future	future	JJ
cord-014597-66vd2mdu	2365	65	perspectives	perspective	NNS
cord-014597-66vd2mdu	2365	66	High	high	JJ
cord-014597-66vd2mdu	2365	67	density	density	NN
cord-014597-66vd2mdu	2365	68	transfection	transfection	NN
cord-014597-66vd2mdu	2365	69	with	with	IN
cord-014597-66vd2mdu	2365	70	HEK	HEK	NNP
cord-014597-66vd2mdu	2365	71	293	293	CD
cord-014597-66vd2mdu	2365	72	cells	cell	NNS
cord-014597-66vd2mdu	2365	73	allows	allow	VBZ
cord-014597-66vd2mdu	2365	74	doubling	double	VBG
cord-014597-66vd2mdu	2365	75	of	of	IN
cord-014597-66vd2mdu	2365	76	transient	transient	JJ
cord-014597-66vd2mdu	2365	77	titers	titer	NNS
cord-014597-66vd2mdu	2365	78	and	and	CC
cord-014597-66vd2mdu	2365	79	removes	remove	VBZ
cord-014597-66vd2mdu	2365	80	need	need	NN
cord-014597-66vd2mdu	2365	81	for	for	IN
cord-014597-66vd2mdu	2365	82	a	a	DT
cord-014597-66vd2mdu	2365	83	priori	priori	FW
cord-014597-66vd2mdu	2365	84	DNA	dna	NN
cord-014597-66vd2mdu	2365	85	complex	complex	JJ
cord-014597-66vd2mdu	2365	86	formation	formation	NN
cord-014597-66vd2mdu	2365	87	with	with	IN
cord-014597-66vd2mdu	2365	88	PEI	PEI	NNP
cord-014597-66vd2mdu	2365	89	Regulation	Regulation	NNP
cord-014597-66vd2mdu	2365	90	of	of	IN
cord-014597-66vd2mdu	2365	91	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2365	92	protein	protein	NN
cord-014597-66vd2mdu	2365	93	expression	expression	NN
cord-014597-66vd2mdu	2365	94	during	during	IN
cord-014597-66vd2mdu	2365	95	CHOBRI	CHOBRI	NNP
cord-014597-66vd2mdu	2365	96	/	/	SYM
cord-014597-66vd2mdu	2365	97	rcTA	rcTA	NNP
cord-014597-66vd2mdu	2365	98	pool	pool	NN
cord-014597-66vd2mdu	2365	99	generation	generation	NN
cord-014597-66vd2mdu	2365	100	increases	increase	VBZ
cord-014597-66vd2mdu	2365	101	productivity	productivity	NN
cord-014597-66vd2mdu	2365	102	and	and	CC
cord-014597-66vd2mdu	2365	103	stability	stability	NN
cord-014597-66vd2mdu	2365	104	QC	QC	NNP
cord-014597-66vd2mdu	2365	105	,	,	,
cord-014597-66vd2mdu	2365	106	Canada	Canada	NNP
cord-014597-66vd2mdu	2365	107	;	;	:
cord-014597-66vd2mdu	2365	108	2	2	CD
cord-014597-66vd2mdu	2365	109	Department	Department	NNP
cord-014597-66vd2mdu	2365	110	of	of	IN
cord-014597-66vd2mdu	2365	111	microbiology	microbiology	NN
cord-014597-66vd2mdu	2365	112	,	,	,
cord-014597-66vd2mdu	2365	113	infectiology	infectiology	NN
cord-014597-66vd2mdu	2365	114	and	and	CC
cord-014597-66vd2mdu	2365	115	immunology	immunology	NN
cord-014597-66vd2mdu	2366	1	The	the	DT
cord-014597-66vd2mdu	2366	2	cumate	cumate	NN
cord-014597-66vd2mdu	2366	3	gene	gene	NN
cord-014597-66vd2mdu	2366	4	-	-	HYPH
cord-014597-66vd2mdu	2366	5	switch	switch	NN
cord-014597-66vd2mdu	2366	6	:	:	:
cord-014597-66vd2mdu	2366	7	a	a	DT
cord-014597-66vd2mdu	2366	8	system	system	NN
cord-014597-66vd2mdu	2366	9	for	for	IN
cord-014597-66vd2mdu	2366	10	regulated	regulated	JJ
cord-014597-66vd2mdu	2366	11	expression	expression	NN
cord-014597-66vd2mdu	2366	12	in	in	IN
cord-014597-66vd2mdu	2366	13	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2366	14	cells	cell	NNS
cord-014597-66vd2mdu	2366	15	Rapid	rapid	JJ
cord-014597-66vd2mdu	2366	16	protein	protein	NN
cord-014597-66vd2mdu	2366	17	production	production	NN
cord-014597-66vd2mdu	2366	18	from	from	IN
cord-014597-66vd2mdu	2366	19	stable	stable	JJ
cord-014597-66vd2mdu	2366	20	CHO	CHO	NNP
cord-014597-66vd2mdu	2366	21	cell	cell	NN
cord-014597-66vd2mdu	2366	22	pools	pool	NNS
cord-014597-66vd2mdu	2366	23	using	use	VBG
cord-014597-66vd2mdu	2366	24	plasmid	plasmid	NN
cord-014597-66vd2mdu	2366	25	vector	vector	NN
cord-014597-66vd2mdu	2366	26	and	and	CC
cord-014597-66vd2mdu	2366	27	the	the	DT
cord-014597-66vd2mdu	2366	28	cumate	cumate	NNP
cord-014597-66vd2mdu	2366	29	gene	gene	NN
cord-014597-66vd2mdu	2366	30	-	-	HYPH
cord-014597-66vd2mdu	2366	31	switch	switch	NN
cord-014597-66vd2mdu	2366	32	Christoph	Christoph	NNP
cord-014597-66vd2mdu	2366	33	Zehe	Zehe	NNP
cord-014597-66vd2mdu	2366	34	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	2366	35	Stedim	Stedim	NNP
cord-014597-66vd2mdu	2366	36	Cellca	Cellca	NNP
cord-014597-66vd2mdu	2366	37	GmbH	GmbH	NNP
cord-014597-66vd2mdu	2366	38	,	,	,
cord-014597-66vd2mdu	2366	39	88471	88471	CD
cord-014597-66vd2mdu	2366	40	Laupheim	Laupheim	NNP
cord-014597-66vd2mdu	2366	41	Animal	Animal	NNP
cord-014597-66vd2mdu	2366	42	Cell	Cell	NNP
cord-014597-66vd2mdu	2366	43	Technology	Technology	NNP
cord-014597-66vd2mdu	2366	44	:	:	:
cord-014597-66vd2mdu	2367	1	From	from	IN
cord-014597-66vd2mdu	2367	2	Target	Target	NNP
cord-014597-66vd2mdu	2367	3	to	to	IN
cord-014597-66vd2mdu	2368	1	Market	Market	NNP
cord-014597-66vd2mdu	2368	2	Recent	recent	JJ
cord-014597-66vd2mdu	2368	3	advances	advance	NNS
cord-014597-66vd2mdu	2368	4	in	in	IN
cord-014597-66vd2mdu	2368	5	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2368	6	protein	protein	NN
cord-014597-66vd2mdu	2368	7	production	production	NN
cord-014597-66vd2mdu	2368	8	Molecular	Molecular	NNP
cord-014597-66vd2mdu	2368	9	mechanisms	mechanism	NNS
cord-014597-66vd2mdu	2368	10	of	of	IN
cord-014597-66vd2mdu	2368	11	RNA	RNA	NNP
cord-014597-66vd2mdu	2368	12	interference	interference	NN
cord-014597-66vd2mdu	2368	13	.	.	.
cord-014597-66vd2mdu	2369	1	Annual	annual	JJ
cord-014597-66vd2mdu	2369	2	review	review	NN
cord-014597-66vd2mdu	2369	3	of	of	IN
cord-014597-66vd2mdu	2369	4	biophysics	biophysics	NNP
cord-014597-66vd2mdu	2369	5	Ribosome	Ribosome	NNP
cord-014597-66vd2mdu	2369	6	profiling	profiling	NN
cord-014597-66vd2mdu	2369	7	-	-	HYPH
cord-014597-66vd2mdu	2369	8	guided	guide	VBN
cord-014597-66vd2mdu	2369	9	depletion	depletion	NN
cord-014597-66vd2mdu	2369	10	of	of	IN
cord-014597-66vd2mdu	2369	11	an	an	DT
cord-014597-66vd2mdu	2369	12	mRNA	mRNA	NNP
cord-014597-66vd2mdu	2369	13	increases	increase	VBZ
cord-014597-66vd2mdu	2369	14	cell	cell	NN
cord-014597-66vd2mdu	2369	15	growth	growth	NN
cord-014597-66vd2mdu	2369	16	rate	rate	NN
cord-014597-66vd2mdu	2369	17	and	and	CC
cord-014597-66vd2mdu	2369	18	protein	protein	NN
cord-014597-66vd2mdu	2369	19	secretion	secretion	NN
cord-014597-66vd2mdu	2370	1	Ubiquitous	Ubiquitous	NNP
cord-014597-66vd2mdu	2370	2	Chromatin	Chromatin	NNP
cord-014597-66vd2mdu	2370	3	-	-	HYPH
cord-014597-66vd2mdu	2370	4	opening	open	VBG
cord-014597-66vd2mdu	2370	5	Elements	element	NNS
cord-014597-66vd2mdu	2370	6	(	(	-LRB-
cord-014597-66vd2mdu	2370	7	UCOEs	ucoe	NNS
cord-014597-66vd2mdu	2370	8	)	)	-RRB-
cord-014597-66vd2mdu	2370	9	:	:	:
cord-014597-66vd2mdu	2371	1	Applications	application	NNS
cord-014597-66vd2mdu	2371	2	in	in	IN
cord-014597-66vd2mdu	2371	3	biomanufacturing	biomanufacturing	NN
cord-014597-66vd2mdu	2371	4	and	and	CC
cord-014597-66vd2mdu	2371	5	gene	gene	NN
cord-014597-66vd2mdu	2371	6	therapy	therapy	NN
cord-014597-66vd2mdu	2372	1	The	the	DT
cord-014597-66vd2mdu	2372	2	art	art	NN
cord-014597-66vd2mdu	2372	3	of	of	IN
cord-014597-66vd2mdu	2372	4	CHO	CHO	NNP
cord-014597-66vd2mdu	2372	5	cell	cell	NN
cord-014597-66vd2mdu	2372	6	engineering	engineering	NN
cord-014597-66vd2mdu	2372	7	:	:	:
cord-014597-66vd2mdu	2373	1	A	a	DT
cord-014597-66vd2mdu	2373	2	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	2373	3	retrospect	retrospect	NN
cord-014597-66vd2mdu	2373	4	and	and	CC
cord-014597-66vd2mdu	2373	5	future	future	JJ
cord-014597-66vd2mdu	2373	6	perspectives	perspective	NNS
cord-014597-66vd2mdu	2374	1	A	a	DT
cord-014597-66vd2mdu	2374	2	novel	novel	NN
cord-014597-66vd2mdu	2374	3	Bxb1	Bxb1	NNP
cord-014597-66vd2mdu	2374	4	integrase	integrase	NN
cord-014597-66vd2mdu	2374	5	RMCE	RMCE	NNP
cord-014597-66vd2mdu	2374	6	system	system	NN
cord-014597-66vd2mdu	2374	7	for	for	IN
cord-014597-66vd2mdu	2374	8	high	high	JJ
cord-014597-66vd2mdu	2374	9	fidelity	fidelity	NN
cord-014597-66vd2mdu	2374	10	site	site	NN
cord-014597-66vd2mdu	2374	11	-	-	HYPH
cord-014597-66vd2mdu	2374	12	specific	specific	JJ
cord-014597-66vd2mdu	2374	13	integration	integration	NN
cord-014597-66vd2mdu	2374	14	of	of	IN
cord-014597-66vd2mdu	2374	15	mAb	mab	JJ
cord-014597-66vd2mdu	2374	16	expression	expression	NN
cord-014597-66vd2mdu	2374	17	cassette	cassette	NN
cord-014597-66vd2mdu	2374	18	in	in	IN
cord-014597-66vd2mdu	2374	19	CHO	CHO	NNP
cord-014597-66vd2mdu	2374	20	Cells	Cells	NNP
cord-014597-66vd2mdu	2374	21	High	High	NNP
cord-014597-66vd2mdu	2374	22	-	-	HYPH
cord-014597-66vd2mdu	2374	23	troughput	troughput	NN
cord-014597-66vd2mdu	2374	24	Lipidomic	Lipidomic	NNP
cord-014597-66vd2mdu	2374	25	and	and	CC
cord-014597-66vd2mdu	2374	26	Transcriptomic	Transcriptomic	NNP
cord-014597-66vd2mdu	2374	27	Analysis	Analysis	NNP
cord-014597-66vd2mdu	2374	28	to	to	TO
cord-014597-66vd2mdu	2374	29	Compare	compare	VB
cord-014597-66vd2mdu	2375	1	SP2/0	SP2/0	NNP
cord-014597-66vd2mdu	2375	2	,	,	,
cord-014597-66vd2mdu	2375	3	CHO	CHO	NNP
cord-014597-66vd2mdu	2375	4	,	,	,
cord-014597-66vd2mdu	2375	5	and	and	CC
cord-014597-66vd2mdu	2375	6	HEK-293	HEK-293	NNP
cord-014597-66vd2mdu	2375	7	Mammalian	Mammalian	NNP
cord-014597-66vd2mdu	2375	8	Cell	Cell	NNP
cord-014597-66vd2mdu	2375	9	Lines	Lines	NNP
cord-014597-66vd2mdu	2375	10	Single	single	JJ
cord-014597-66vd2mdu	2375	11	cell	cell	NN
cord-014597-66vd2mdu	2375	12	characterisation	characterisation	NN
cord-014597-66vd2mdu	2375	13	of	of	IN
cord-014597-66vd2mdu	2375	14	Chinese	chinese	JJ
cord-014597-66vd2mdu	2375	15	hamster	hamster	NN
cord-014597-66vd2mdu	2375	16	ovary	ovary	NN
cord-014597-66vd2mdu	2375	17	(	(	-LRB-
cord-014597-66vd2mdu	2375	18	CHO	CHO	NNP
cord-014597-66vd2mdu	2375	19	)	)	-RRB-
cord-014597-66vd2mdu	2375	20	cells	cell	NNS
cord-014597-66vd2mdu	2375	21	Eva	Eva	NNP
cord-014597-66vd2mdu	2375	22	Pekle	Pekle	NNP
cord-014597-66vd2mdu	2375	23	1,2	1,2	CD
cord-014597-66vd2mdu	2375	24	,	,	,
cord-014597-66vd2mdu	2375	25	Guglielmo	Guglielmo	NNP
cord-014597-66vd2mdu	2375	26	Rosignoli	Rosignoli	NNP
cord-014597-66vd2mdu	2376	1	1	1	LS
cord-014597-66vd2mdu	2376	2	Generation	generation	NN
cord-014597-66vd2mdu	2376	3	of	of	IN
cord-014597-66vd2mdu	2376	4	stable	stable	JJ
cord-014597-66vd2mdu	2376	5	Chinese	chinese	JJ
cord-014597-66vd2mdu	2376	6	hamster	hamster	NN
cord-014597-66vd2mdu	2376	7	ovary	ovary	NN
cord-014597-66vd2mdu	2376	8	pools	pool	NNS
cord-014597-66vd2mdu	2376	9	yielding	yield	VBG
cord-014597-66vd2mdu	2376	10	antibody	antibody	NN
cord-014597-66vd2mdu	2376	11	titers	titer	NNS
cord-014597-66vd2mdu	2376	12	of	of	IN
cord-014597-66vd2mdu	2376	13	up	up	IN
cord-014597-66vd2mdu	2376	14	to	to	TO
cord-014597-66vd2mdu	2376	15	7.6	7.6	CD
cord-014597-66vd2mdu	2376	16	g	g	NN
cord-014597-66vd2mdu	2376	17	/	/	SYM
cord-014597-66vd2mdu	2376	18	L	l	NN
cord-014597-66vd2mdu	2376	19	using	use	VBG
cord-014597-66vd2mdu	2376	20	the	the	DT
cord-014597-66vd2mdu	2376	21	piggyBac	piggybac	JJ
cord-014597-66vd2mdu	2376	22	transposon	transposon	NN
cord-014597-66vd2mdu	2376	23	system	system	NN
cord-014597-66vd2mdu	2376	24	Comparison	Comparison	NNP
cord-014597-66vd2mdu	2376	25	of	of	IN
cord-014597-66vd2mdu	2376	26	three	three	CD
cord-014597-66vd2mdu	2376	27	transposons	transposon	NNS
cord-014597-66vd2mdu	2376	28	for	for	IN
cord-014597-66vd2mdu	2376	29	the	the	DT
cord-014597-66vd2mdu	2376	30	generation	generation	NN
cord-014597-66vd2mdu	2376	31	of	of	IN
cord-014597-66vd2mdu	2376	32	highly	highly	RB
cord-014597-66vd2mdu	2376	33	productive	productive	JJ
cord-014597-66vd2mdu	2376	34	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2376	35	CHO	CHO	NNP
cord-014597-66vd2mdu	2376	36	cell	cell	NN
cord-014597-66vd2mdu	2376	37	pools	pool	NNS
cord-014597-66vd2mdu	2376	38	and	and	CC
cord-014597-66vd2mdu	2376	39	cell	cell	NN
cord-014597-66vd2mdu	2376	40	lines	line	NNS
cord-014597-66vd2mdu	2376	41	Effects	effect	NNS
cord-014597-66vd2mdu	2376	42	of	of	IN
cord-014597-66vd2mdu	2376	43	ammonia	ammonia	NN
cord-014597-66vd2mdu	2376	44	and	and	CC
cord-014597-66vd2mdu	2376	45	lactate	lactate	NN
cord-014597-66vd2mdu	2376	46	on	on	IN
cord-014597-66vd2mdu	2376	47	hybridoma	hybridoma	NN
cord-014597-66vd2mdu	2376	48	growth	growth	NN
cord-014597-66vd2mdu	2376	49	,	,	,
cord-014597-66vd2mdu	2376	50	metabolism	metabolism	NN
cord-014597-66vd2mdu	2376	51	,	,	,
cord-014597-66vd2mdu	2376	52	and	and	CC
cord-014597-66vd2mdu	2376	53	antibody	antibody	NN
cord-014597-66vd2mdu	2376	54	production	production	NN
cord-014597-66vd2mdu	2376	55	Lactate	lactate	NN
cord-014597-66vd2mdu	2376	56	and	and	CC
cord-014597-66vd2mdu	2376	57	glucose	glucose	NN
cord-014597-66vd2mdu	2376	58	concomitant	concomitant	JJ
cord-014597-66vd2mdu	2376	59	consumption	consumption	NN
cord-014597-66vd2mdu	2376	60	as	as	IN
cord-014597-66vd2mdu	2376	61	a	a	DT
cord-014597-66vd2mdu	2376	62	self	self	NN
cord-014597-66vd2mdu	2376	63	-	-	HYPH
cord-014597-66vd2mdu	2376	64	regulated	regulate	VBN
cord-014597-66vd2mdu	2376	65	pH	pH	NNP
cord-014597-66vd2mdu	2376	66	detoxification	detoxification	NN
cord-014597-66vd2mdu	2376	67	mechanism	mechanism	NN
cord-014597-66vd2mdu	2376	68	in	in	IN
cord-014597-66vd2mdu	2376	69	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2376	70	cell	cell	NN
cord-014597-66vd2mdu	2376	71	cultures	culture	NNS
cord-014597-66vd2mdu	2377	1	Flux	flux	NN
cord-014597-66vd2mdu	2377	2	balance	balance	NN
cord-014597-66vd2mdu	2377	3	analysis	analysis	NN
cord-014597-66vd2mdu	2377	4	of	of	IN
cord-014597-66vd2mdu	2377	5	CHO	CHO	NNP
cord-014597-66vd2mdu	2377	6	cells	cell	NNS
cord-014597-66vd2mdu	2377	7	before	before	RB
cord-014597-66vd2mdu	2377	8	and	and	CC
cord-014597-66vd2mdu	2377	9	after	after	IN
cord-014597-66vd2mdu	2377	10	a	a	DT
cord-014597-66vd2mdu	2377	11	metabolic	metabolic	JJ
cord-014597-66vd2mdu	2377	12	switch	switch	NN
cord-014597-66vd2mdu	2377	13	from	from	IN
cord-014597-66vd2mdu	2377	14	lactate	lactate	NN
cord-014597-66vd2mdu	2377	15	production	production	NN
cord-014597-66vd2mdu	2377	16	to	to	IN
cord-014597-66vd2mdu	2377	17	consumption	consumption	NN
cord-014597-66vd2mdu	2377	18	Reducing	reduce	VBG
cord-014597-66vd2mdu	2377	19	Recon	Recon	NNP
cord-014597-66vd2mdu	2377	20	2	2	CD
cord-014597-66vd2mdu	2377	21	for	for	IN
cord-014597-66vd2mdu	2377	22	steady	steady	JJ
cord-014597-66vd2mdu	2377	23	-	-	HYPH
cord-014597-66vd2mdu	2377	24	state	state	NN
cord-014597-66vd2mdu	2377	25	flux	flux	NN
cord-014597-66vd2mdu	2377	26	analysis	analysis	NN
cord-014597-66vd2mdu	2377	27	of	of	IN
cord-014597-66vd2mdu	2377	28	HEK	HEK	NNP
cord-014597-66vd2mdu	2377	29	cell	cell	NN
cord-014597-66vd2mdu	2377	30	culture	culture	NN
cord-014597-66vd2mdu	2378	1	Trastuzumab	Trastuzumab	NNP
cord-014597-66vd2mdu	2378	2	-Mechanism	-mechanism	NN
cord-014597-66vd2mdu	2378	3	of	of	IN
cord-014597-66vd2mdu	2378	4	action	action	NN
cord-014597-66vd2mdu	2378	5	and	and	CC
cord-014597-66vd2mdu	2378	6	use	use	NN
cord-014597-66vd2mdu	2378	7	in	in	IN
cord-014597-66vd2mdu	2378	8	clinical	clinical	JJ
cord-014597-66vd2mdu	2378	9	practice	practice	NN
cord-014597-66vd2mdu	2378	10	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2378	11	cell	cell	NN
cord-014597-66vd2mdu	2378	12	culture	culture	NN
cord-014597-66vd2mdu	2378	13	media	medium	NNS
cord-014597-66vd2mdu	2378	14	study	study	NN
cord-014597-66vd2mdu	2378	15	towards	towards	IN
cord-014597-66vd2mdu	2378	16	bioprocess	bioprocess	NN
cord-014597-66vd2mdu	2378	17	optimization	optimization	NN
cord-014597-66vd2mdu	2378	18	:	:	:
cord-014597-66vd2mdu	2378	19	Animal	animal	NN
cord-014597-66vd2mdu	2378	20	derived	derive	VBN
cord-014597-66vd2mdu	2378	21	component	component	NN
cord-014597-66vd2mdu	2378	22	free	free	JJ
cord-014597-66vd2mdu	2378	23	and	and	CC
cord-014597-66vd2mdu	2378	24	animal	animal	NN
cord-014597-66vd2mdu	2378	25	derived	derive	VBN
cord-014597-66vd2mdu	2378	26	component	component	NN
cord-014597-66vd2mdu	2378	27	containing	contain	VBG
cord-014597-66vd2mdu	2378	28	platforms	platform	NNS
cord-014597-66vd2mdu	2378	29	Enhancing	enhance	VBG
cord-014597-66vd2mdu	2378	30	heterologous	heterologous	JJ
cord-014597-66vd2mdu	2378	31	protein	protein	NN
cord-014597-66vd2mdu	2378	32	expression	expression	NN
cord-014597-66vd2mdu	2378	33	and	and	CC
cord-014597-66vd2mdu	2378	34	secretion	secretion	NN
cord-014597-66vd2mdu	2378	35	in	in	IN
cord-014597-66vd2mdu	2378	36	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2378	37	cells	cell	NNS
cord-014597-66vd2mdu	2378	38	by	by	IN
cord-014597-66vd2mdu	2378	39	means	mean	NNS
cord-014597-66vd2mdu	2378	40	of	of	IN
cord-014597-66vd2mdu	2378	41	combination	combination	NN
cord-014597-66vd2mdu	2378	42	of	of	IN
cord-014597-66vd2mdu	2378	43	CMV	CMV	NNP
cord-014597-66vd2mdu	2378	44	promoter	promoter	NN
cord-014597-66vd2mdu	2378	45	and	and	CC
cord-014597-66vd2mdu	2378	46	IFNα2	ifnα2	JJ
cord-014597-66vd2mdu	2378	47	signal	signal	NN
cord-014597-66vd2mdu	2378	48	peptide	peptide	NN
cord-014597-66vd2mdu	2378	49	Production	Production	NNP
cord-014597-66vd2mdu	2378	50	of	of	IN
cord-014597-66vd2mdu	2378	51	lentiviral	lentiviral	JJ
cord-014597-66vd2mdu	2378	52	vectors	vector	NNS
cord-014597-66vd2mdu	2379	1	References	reference	NNS
cord-014597-66vd2mdu	2379	2	1	1	CD
cord-014597-66vd2mdu	2379	3	.	.	.
cord-014597-66vd2mdu	2380	1	Wurm	Wurm	NNP
cord-014597-66vd2mdu	2380	2	F	F	NNP
cord-014597-66vd2mdu	2380	3	M	M	NNP
cord-014597-66vd2mdu	2380	4	:	:	:
cord-014597-66vd2mdu	2380	5	Production	production	NN
cord-014597-66vd2mdu	2380	6	of	of	IN
cord-014597-66vd2mdu	2380	7	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2380	8	protein	protein	NN
cord-014597-66vd2mdu	2380	9	therapeutics	therapeutic	NNS
cord-014597-66vd2mdu	2380	10	in	in	IN
cord-014597-66vd2mdu	2380	11	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2380	12	mamammalian	mamammalian	NN
cord-014597-66vd2mdu	2380	13	cells	cell	NNS
cord-014597-66vd2mdu	2380	14	Initial	initial	JJ
cord-014597-66vd2mdu	2380	15	identification	identification	NN
cord-014597-66vd2mdu	2380	16	of	of	IN
cord-014597-66vd2mdu	2380	17	low	low	JJ
cord-014597-66vd2mdu	2380	18	temperature	temperature	NN
cord-014597-66vd2mdu	2380	19	and	and	CC
cord-014597-66vd2mdu	2380	20	culture	culture	NN
cord-014597-66vd2mdu	2380	21	stage	stage	NN
cord-014597-66vd2mdu	2380	22	induction	induction	NN
cord-014597-66vd2mdu	2380	23	of	of	IN
cord-014597-66vd2mdu	2380	24	miRNA	miRNA	NNP
cord-014597-66vd2mdu	2380	25	expression	expression	NN
cord-014597-66vd2mdu	2380	26	in	in	IN
cord-014597-66vd2mdu	2380	27	suspension	suspension	NN
cord-014597-66vd2mdu	2380	28	CHO	CHO	NNP
cord-014597-66vd2mdu	2380	29	-	-	HYPH
cord-014597-66vd2mdu	2380	30	K1	K1	NNP
cord-014597-66vd2mdu	2380	31	cells	cell	NNS
cord-014597-66vd2mdu	2380	32	Small	small	JJ
cord-014597-66vd2mdu	2380	33	indels	indel	NNS
cord-014597-66vd2mdu	2380	34	induced	induce	VBN
cord-014597-66vd2mdu	2380	35	by	by	IN
cord-014597-66vd2mdu	2380	36	CRISPR	CRISPR	NNP
cord-014597-66vd2mdu	2380	37	/	/	SYM
cord-014597-66vd2mdu	2380	38	Cas9	Cas9	NNP
cord-014597-66vd2mdu	2380	39	in	in	IN
cord-014597-66vd2mdu	2380	40	the	the	DT
cord-014597-66vd2mdu	2380	41	5	5	CD
cord-014597-66vd2mdu	2380	42	'	'	''
cord-014597-66vd2mdu	2380	43	region	region	NN
cord-014597-66vd2mdu	2380	44	of	of	IN
cord-014597-66vd2mdu	2380	45	microRNA	microRNA	NNP
cord-014597-66vd2mdu	2380	46	lead	lead	NN
cord-014597-66vd2mdu	2380	47	to	to	IN
cord-014597-66vd2mdu	2380	48	its	-PRON-	PRP$
cord-014597-66vd2mdu	2380	49	depletion	depletion	NN
cord-014597-66vd2mdu	2380	50	and	and	CC
cord-014597-66vd2mdu	2380	51	Drosha	Drosha	NNP
cord-014597-66vd2mdu	2380	52	processing	processing	NN
cord-014597-66vd2mdu	2380	53	retardance	retardance	NN
cord-014597-66vd2mdu	2380	54	Production	production	NN
cord-014597-66vd2mdu	2380	55	of	of	IN
cord-014597-66vd2mdu	2380	56	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2380	57	protein	protein	NN
cord-014597-66vd2mdu	2380	58	therapeutics	therapeutic	NNS
cord-014597-66vd2mdu	2380	59	in	in	IN
cord-014597-66vd2mdu	2380	60	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2380	61	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2380	62	cells	cell	NNS
cord-014597-66vd2mdu	2380	63	Improved	improve	VBN
cord-014597-66vd2mdu	2380	64	antibody	antibody	NN
cord-014597-66vd2mdu	2380	65	production	production	NN
cord-014597-66vd2mdu	2380	66	in	in	IN
cord-014597-66vd2mdu	2380	67	Chinese	chinese	JJ
cord-014597-66vd2mdu	2380	68	hamster	hamster	NN
cord-014597-66vd2mdu	2380	69	ovary	ovary	NN
cord-014597-66vd2mdu	2380	70	cells	cell	NNS
cord-014597-66vd2mdu	2380	71	by	by	IN
cord-014597-66vd2mdu	2380	72	ATF4	ATF4	NNP
cord-014597-66vd2mdu	2380	73	overexpression	overexpression	NN
cord-014597-66vd2mdu	2381	1	The	the	DT
cord-014597-66vd2mdu	2381	2	vesicle	vesicle	NN
cord-014597-66vd2mdu	2381	3	-	-	HYPH
cord-014597-66vd2mdu	2381	4	trafficking	traffic	VBG
cord-014597-66vd2mdu	2381	5	protein	protein	NN
cord-014597-66vd2mdu	2381	6	munc18b	munc18b	NNP
cord-014597-66vd2mdu	2381	7	increases	increase	VBZ
cord-014597-66vd2mdu	2381	8	the	the	DT
cord-014597-66vd2mdu	2381	9	secretory	secretory	JJ
cord-014597-66vd2mdu	2381	10	capacity	capacity	NN
cord-014597-66vd2mdu	2381	11	of	of	IN
cord-014597-66vd2mdu	2381	12	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2381	13	cells	cell	NNS
cord-014597-66vd2mdu	2381	14	Rapid	rapid	JJ
cord-014597-66vd2mdu	2381	15	evaluation	evaluation	NN
cord-014597-66vd2mdu	2381	16	of	of	IN
cord-014597-66vd2mdu	2381	17	N	n	CD
cord-014597-66vd2mdu	2381	18	-	-	HYPH
cord-014597-66vd2mdu	2381	19	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	2381	20	status	status	NN
cord-014597-66vd2mdu	2381	21	of	of	IN
cord-014597-66vd2mdu	2381	22	antibodies	antibody	NNS
cord-014597-66vd2mdu	2381	23	with	with	IN
cord-014597-66vd2mdu	2381	24	chemiluminescent	chemiluminescent	NN
cord-014597-66vd2mdu	2381	25	lectin	lectin	NN
cord-014597-66vd2mdu	2381	26	-	-	HYPH
cord-014597-66vd2mdu	2381	27	binding	bind	VBG
cord-014597-66vd2mdu	2381	28	assay	assay	NN
cord-014597-66vd2mdu	2382	1	Intracellular	Intracellular	NNP
cord-014597-66vd2mdu	2382	2	secretion	secretion	NN
cord-014597-66vd2mdu	2382	3	pathway	pathway	NN
cord-014597-66vd2mdu	2382	4	analysis	analysis	NN
cord-014597-66vd2mdu	2382	5	for	for	IN
cord-014597-66vd2mdu	2382	6	constructing	construct	VBG
cord-014597-66vd2mdu	2382	7	highly	highly	RB
cord-014597-66vd2mdu	2382	8	producible	producible	JJ
cord-014597-66vd2mdu	2382	9	engineered	engineer	VBN
cord-014597-66vd2mdu	2382	10	CHO	CHO	NNP
cord-014597-66vd2mdu	2382	11	cells	cell	NNS
cord-014597-66vd2mdu	2382	12	.	.	.
cord-014597-66vd2mdu	2383	1	16th	16th	NNP
cord-014597-66vd2mdu	2383	2	annual	annual	NNP
cord-014597-66vd2mdu	2383	3	peptalk	peptalk	NNP
cord-014597-66vd2mdu	2383	4	Intracellular	Intracellular	NNP
cord-014597-66vd2mdu	2383	5	secretion	secretion	NN
cord-014597-66vd2mdu	2383	6	analysis	analysis	NN
cord-014597-66vd2mdu	2383	7	of	of	IN
cord-014597-66vd2mdu	2383	8	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2383	9	antibodies	antibody	NNS
cord-014597-66vd2mdu	2383	10	in	in	IN
cord-014597-66vd2mdu	2383	11	engineered	engineer	VBN
cord-014597-66vd2mdu	2383	12	high	high	RB
cord-014597-66vd2mdu	2383	13	-	-	HYPH
cord-014597-66vd2mdu	2383	14	producible	producible	JJ
cord-014597-66vd2mdu	2383	15	CHO	CHO	NNP
cord-014597-66vd2mdu	2383	16	cells	cell	NNS
cord-014597-66vd2mdu	2383	17	Analysis	Analysis	NNP
cord-014597-66vd2mdu	2383	18	of	of	IN
cord-014597-66vd2mdu	2383	19	intracellular	intracellular	JJ
cord-014597-66vd2mdu	2383	20	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2383	21	IgG	igg	NN
cord-014597-66vd2mdu	2383	22	secretion	secretion	NN
cord-014597-66vd2mdu	2383	23	in	in	IN
cord-014597-66vd2mdu	2383	24	engineered	engineer	VBN
cord-014597-66vd2mdu	2383	25	CHO	CHO	NNP
cord-014597-66vd2mdu	2383	26	cells	cell	NNS
cord-014597-66vd2mdu	2383	27	.	.	.
cord-014597-66vd2mdu	2384	1	The	the	DT
cord-014597-66vd2mdu	2384	2	29th	29th	JJ
cord-014597-66vd2mdu	2384	3	annual	annual	JJ
cord-014597-66vd2mdu	2384	4	and	and	CC
cord-014597-66vd2mdu	2384	5	international	international	JJ
cord-014597-66vd2mdu	2384	6	meeting	meeting	NN
cord-014597-66vd2mdu	2384	7	of	of	IN
cord-014597-66vd2mdu	2384	8	the	the	DT
cord-014597-66vd2mdu	2384	9	Japanese	Japanese	NNP
cord-014597-66vd2mdu	2384	10	Association	Association	NNP
cord-014597-66vd2mdu	2384	11	for	for	IN
cord-014597-66vd2mdu	2384	12	animal	animal	NN
cord-014597-66vd2mdu	2384	13	cell	cell	NN
cord-014597-66vd2mdu	2384	14	technology	technology	NN
cord-014597-66vd2mdu	2384	15	Humanization	Humanization	NNP
cord-014597-66vd2mdu	2384	16	strategies	strategy	NNS
cord-014597-66vd2mdu	2384	17	for	for	IN
cord-014597-66vd2mdu	2384	18	an	an	DT
cord-014597-66vd2mdu	2384	19	anti	anti	JJ
cord-014597-66vd2mdu	2384	20	-	-	JJ
cord-014597-66vd2mdu	2384	21	idiotypic	idiotypic	JJ
cord-014597-66vd2mdu	2384	22	antibody	antibody	NN
cord-014597-66vd2mdu	2384	23	mimicking	mimic	VBG
cord-014597-66vd2mdu	2384	24	HIV	HIV	NNP
cord-014597-66vd2mdu	2384	25	-	-	HYPH
cord-014597-66vd2mdu	2384	26	I	I	NNP
cord-014597-66vd2mdu	2384	27	gp41	gp41	NNP
cord-014597-66vd2mdu	2384	28	Antibody	Antibody	NNP
cord-014597-66vd2mdu	2384	29	humanization	humanization	NN
cord-014597-66vd2mdu	2384	30	by	by	IN
cord-014597-66vd2mdu	2384	31	molecular	molecular	JJ
cord-014597-66vd2mdu	2384	32	dynamics	dynamic	NNS
cord-014597-66vd2mdu	2384	33	simulations	simulation	NNS
cord-014597-66vd2mdu	2384	34	-	-	HYPH
cord-014597-66vd2mdu	2384	35	in	in	IN
cord-014597-66vd2mdu	2384	36	-	-	HYPH
cord-014597-66vd2mdu	2384	37	silico	silico	NN
cord-014597-66vd2mdu	2384	38	guided	guide	VBN
cord-014597-66vd2mdu	2384	39	selection	selection	NN
cord-014597-66vd2mdu	2384	40	of	of	IN
cord-014597-66vd2mdu	2384	41	critical	critical	JJ
cord-014597-66vd2mdu	2384	42	backmutations	backmutation	NNS
cord-014597-66vd2mdu	2384	43	MaxQuant	MaxQuant	NNP
cord-014597-66vd2mdu	2384	44	enables	enable	VBZ
cord-014597-66vd2mdu	2384	45	high	high	JJ
cord-014597-66vd2mdu	2384	46	peptide	peptide	NN
cord-014597-66vd2mdu	2384	47	identification	identification	NN
cord-014597-66vd2mdu	2384	48	rates	rate	NNS
cord-014597-66vd2mdu	2384	49	,	,	,
cord-014597-66vd2mdu	2384	50	individualized	individualized	JJ
cord-014597-66vd2mdu	2384	51	p.p.b.-range	p.p.b.-range	IN
cord-014597-66vd2mdu	2384	52	mass	mass	NN
cord-014597-66vd2mdu	2384	53	accuracies	accuracy	NNS
cord-014597-66vd2mdu	2384	54	and	and	CC
cord-014597-66vd2mdu	2384	55	proteome	proteome	NN
cord-014597-66vd2mdu	2384	56	-	-	HYPH
cord-014597-66vd2mdu	2384	57	wide	wide	JJ
cord-014597-66vd2mdu	2384	58	protein	protein	NN
cord-014597-66vd2mdu	2384	59	quantification	quantification	NN
cord-014597-66vd2mdu	2385	1	The	the	DT
cord-014597-66vd2mdu	2385	2	Perseus	Perseus	NNP
cord-014597-66vd2mdu	2385	3	computational	computational	JJ
cord-014597-66vd2mdu	2385	4	platform	platform	NN
cord-014597-66vd2mdu	2385	5	for	for	IN
cord-014597-66vd2mdu	2385	6	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	2385	7	analysis	analysis	NN
cord-014597-66vd2mdu	2385	8	of	of	IN
cord-014597-66vd2mdu	2386	1	(	(	-LRB-
cord-014597-66vd2mdu	2386	2	prote)omics	prote)omics	NNP
cord-014597-66vd2mdu	2386	3	data	datum	NNS
cord-014597-66vd2mdu	2386	4	Hoffrogge	hoffrogge	NN
cord-014597-66vd2mdu	2386	5	:	:	:
cord-014597-66vd2mdu	2386	6	Label	label	NN
cord-014597-66vd2mdu	2386	7	-	-	HYPH
cord-014597-66vd2mdu	2386	8	free	free	JJ
cord-014597-66vd2mdu	2386	9	protein	protein	NN
cord-014597-66vd2mdu	2386	10	quantification	quantification	NN
cord-014597-66vd2mdu	2386	11	of	of	IN
cord-014597-66vd2mdu	2386	12	sodium	sodium	NN
cord-014597-66vd2mdu	2386	13	butyrate	butyrate	NN
cord-014597-66vd2mdu	2386	14	treated	treat	VBD
cord-014597-66vd2mdu	2386	15	CHO	CHO	NNP
cord-014597-66vd2mdu	2386	16	cells	cell	NNS
cord-014597-66vd2mdu	2386	17	by	by	IN
cord-014597-66vd2mdu	2386	18	ESI	ESI	NNP
cord-014597-66vd2mdu	2386	19	-	-	HYPH
cord-014597-66vd2mdu	2386	20	UHR	UHR	NNP
cord-014597-66vd2mdu	2386	21	-	-	HYPH
cord-014597-66vd2mdu	2386	22	TOF	TOF	NNP
cord-014597-66vd2mdu	2386	23	-	-	HYPH
cord-014597-66vd2mdu	2386	24	MS	MS	NNP
cord-014597-66vd2mdu	2387	1	The	the	DT
cord-014597-66vd2mdu	2387	2	art	art	NN
cord-014597-66vd2mdu	2387	3	of	of	IN
cord-014597-66vd2mdu	2387	4	CHO	CHO	NNP
cord-014597-66vd2mdu	2387	5	cell	cell	NN
cord-014597-66vd2mdu	2387	6	engineering	engineering	NN
cord-014597-66vd2mdu	2387	7	:	:	:
cord-014597-66vd2mdu	2387	8	a	a	DT
cord-014597-66vd2mdu	2387	9	comprehensive	comprehensive	JJ
cord-014597-66vd2mdu	2387	10	retrospect	retrospect	NN
cord-014597-66vd2mdu	2387	11	and	and	CC
cord-014597-66vd2mdu	2387	12	future	future	JJ
cord-014597-66vd2mdu	2387	13	perspectives	perspective	NNS
cord-014597-66vd2mdu	2387	14	GS	GS	NNP
cord-014597-66vd2mdu	2387	15	system	system	NN
cord-014597-66vd2mdu	2387	16	for	for	IN
cord-014597-66vd2mdu	2387	17	increased	increased	JJ
cord-014597-66vd2mdu	2387	18	expression	expression	NN
cord-014597-66vd2mdu	2387	19	of	of	IN
cord-014597-66vd2mdu	2387	20	difficult	difficult	JJ
cord-014597-66vd2mdu	2387	21	-	-	HYPH
cord-014597-66vd2mdu	2387	22	to	to	TO
cord-014597-66vd2mdu	2387	23	-	-	HYPH
cord-014597-66vd2mdu	2387	24	express	express	NN
cord-014597-66vd2mdu	2387	25	proteins	protein	NNS
cord-014597-66vd2mdu	2388	1	The	the	DT
cord-014597-66vd2mdu	2388	2	Netherlands	Netherlands	NNP
cord-014597-66vd2mdu	2388	3	Correspondence	Correspondence	NNP
cord-014597-66vd2mdu	2388	4	:	:	:
cord-014597-66vd2mdu	2388	5	Maurice	Maurice	NNP
cord-014597-66vd2mdu	2388	6	van	van	NNP
cord-014597-66vd2mdu	2388	7	der	der	NNP
cord-014597-66vd2mdu	2388	8	Heijden	Heijden	NNP
cord-014597-66vd2mdu	2388	9	(	(	-LRB-
cord-014597-66vd2mdu	2388	10	heijden@proteonic.nl	heijden@proteonic.nl	''
cord-014597-66vd2mdu	2388	11	)	)	-RRB-
cord-014597-66vd2mdu	2389	1	BMC	BMC	NNP
cord-014597-66vd2mdu	2389	2	Proceedings	Proceedings	NNP
cord-014597-66vd2mdu	2389	3	Increased	increase	VBD
cord-014597-66vd2mdu	2389	4	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2389	5	protein	protein	NN
cord-014597-66vd2mdu	2389	6	production	production	NN
cord-014597-66vd2mdu	2389	7	owing	owe	VBG
cord-014597-66vd2mdu	2389	8	to	to	IN
cord-014597-66vd2mdu	2389	9	expanded	expand	VBN
cord-014597-66vd2mdu	2389	10	opportunities	opportunity	NNS
cord-014597-66vd2mdu	2389	11	for	for	IN
cord-014597-66vd2mdu	2389	12	vector	vector	NN
cord-014597-66vd2mdu	2389	13	integration	integration	NN
cord-014597-66vd2mdu	2389	14	in	in	IN
cord-014597-66vd2mdu	2389	15	high	high	JJ
cord-014597-66vd2mdu	2389	16	chromosome	chromosome	NN
cord-014597-66vd2mdu	2389	17	number	number	NN
cord-014597-66vd2mdu	2389	18	Chinese	chinese	JJ
cord-014597-66vd2mdu	2389	19	hamster	hamster	NN
cord-014597-66vd2mdu	2389	20	ovary	ovary	NN
cord-014597-66vd2mdu	2389	21	cells	cell	NNS
cord-014597-66vd2mdu	2389	22	IRES	IRES	NNP
cord-014597-66vd2mdu	2389	23	-	-	HYPH
cord-014597-66vd2mdu	2389	24	mediated	mediate	VBN
cord-014597-66vd2mdu	2389	25	translation	translation	NN
cord-014597-66vd2mdu	2389	26	of	of	IN
cord-014597-66vd2mdu	2389	27	membrane	membrane	NN
cord-014597-66vd2mdu	2389	28	proteins	protein	NNS
cord-014597-66vd2mdu	2389	29	and	and	CC
cord-014597-66vd2mdu	2389	30	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	2389	31	in	in	IN
cord-014597-66vd2mdu	2389	32	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	2389	33	cell	cell	NN
cord-014597-66vd2mdu	2389	34	-	-	HYPH
cord-014597-66vd2mdu	2389	35	free	free	JJ
cord-014597-66vd2mdu	2389	36	systems	system	NNS
cord-014597-66vd2mdu	2389	37	Cell	cell	NN
cord-014597-66vd2mdu	2389	38	-	-	HYPH
cord-014597-66vd2mdu	2389	39	free	free	JJ
cord-014597-66vd2mdu	2389	40	protein	protein	NN
cord-014597-66vd2mdu	2389	41	expression	expression	NN
cord-014597-66vd2mdu	2389	42	based	base	VBN
cord-014597-66vd2mdu	2389	43	on	on	IN
cord-014597-66vd2mdu	2389	44	extracts	extract	NNS
cord-014597-66vd2mdu	2389	45	from	from	IN
cord-014597-66vd2mdu	2389	46	CHO	CHO	NNP
cord-014597-66vd2mdu	2389	47	cells	cell	NNS
cord-014597-66vd2mdu	2389	48	Comparison	comparison	NN
cord-014597-66vd2mdu	2389	49	of	of	IN
cord-014597-66vd2mdu	2389	50	cell	cell	NN
cord-014597-66vd2mdu	2389	51	-	-	HYPH
cord-014597-66vd2mdu	2389	52	based	base	VBN
cord-014597-66vd2mdu	2389	53	vs.	vs.	IN
cord-014597-66vd2mdu	2389	54	cell	cell	NN
cord-014597-66vd2mdu	2389	55	-	-	HYPH
cord-014597-66vd2mdu	2389	56	free	free	JJ
cord-014597-66vd2mdu	2389	57	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2389	58	systems	system	NNS
cord-014597-66vd2mdu	2389	59	for	for	IN
cord-014597-66vd2mdu	2389	60	the	the	DT
cord-014597-66vd2mdu	2389	61	production	production	NN
cord-014597-66vd2mdu	2389	62	of	of	IN
cord-014597-66vd2mdu	2389	63	a	a	DT
cord-014597-66vd2mdu	2389	64	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2389	65	human	human	JJ
cord-014597-66vd2mdu	2389	66	bone	bone	NN
cord-014597-66vd2mdu	2389	67	morphogenic	morphogenic	JJ
cord-014597-66vd2mdu	2389	68	growth	growth	NN
cord-014597-66vd2mdu	2389	69	factor	factor	NN
cord-014597-66vd2mdu	2389	70	.	.	.
cord-014597-66vd2mdu	2390	1	Eng	Eng	NNP
cord-014597-66vd2mdu	2390	2	Dual	dual	JJ
cord-014597-66vd2mdu	2390	3	-	-	HYPH
cord-014597-66vd2mdu	2390	4	responsive	responsive	JJ
cord-014597-66vd2mdu	2390	5	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2390	6	core	core	NN
cord-014597-66vd2mdu	2390	7	-	-	HYPH
cord-014597-66vd2mdu	2390	8	shell	shell	NN
cord-014597-66vd2mdu	2390	9	nanoparticles	nanoparticle	NNS
cord-014597-66vd2mdu	2390	10	for	for	IN
cord-014597-66vd2mdu	2390	11	nonviral	nonviral	JJ
cord-014597-66vd2mdu	2390	12	gene	gene	NN
cord-014597-66vd2mdu	2390	13	delivery	delivery	NN
cord-014597-66vd2mdu	2390	14	and	and	CC
cord-014597-66vd2mdu	2390	15	cell	cell	NN
cord-014597-66vd2mdu	2390	16	separation	separation	NN
cord-014597-66vd2mdu	2390	17	PDMAEMA	PDMAEMA	NNP
cord-014597-66vd2mdu	2390	18	-	-	HYPH
cord-014597-66vd2mdu	2390	19	grafted	graft	VBN
cord-014597-66vd2mdu	2390	20	core	core	NN
cord-014597-66vd2mdu	2390	21	-	-	HYPH
cord-014597-66vd2mdu	2390	22	shell	shell	NN
cord-014597-66vd2mdu	2390	23	-	-	HYPH
cord-014597-66vd2mdu	2390	24	corona	corona	NN
cord-014597-66vd2mdu	2390	25	particles	particle	NNS
cord-014597-66vd2mdu	2390	26	for	for	IN
cord-014597-66vd2mdu	2390	27	nonviral	nonviral	JJ
cord-014597-66vd2mdu	2390	28	gene	gene	NN
cord-014597-66vd2mdu	2390	29	delivery	delivery	NN
cord-014597-66vd2mdu	2390	30	and	and	CC
cord-014597-66vd2mdu	2390	31	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2390	32	cell	cell	NN
cord-014597-66vd2mdu	2390	33	separation	separation	NN
cord-014597-66vd2mdu	2390	34	Influence	influence	NN
cord-014597-66vd2mdu	2390	35	of	of	IN
cord-014597-66vd2mdu	2390	36	polyplex	polyplex	NNP
cord-014597-66vd2mdu	2390	37	formation	formation	NN
cord-014597-66vd2mdu	2390	38	on	on	IN
cord-014597-66vd2mdu	2390	39	the	the	DT
cord-014597-66vd2mdu	2390	40	performance	performance	NN
cord-014597-66vd2mdu	2390	41	of	of	IN
cord-014597-66vd2mdu	2390	42	starshaped	starshaped	NNP
cord-014597-66vd2mdu	2390	43	polycationic	polycationic	NNP
cord-014597-66vd2mdu	2390	44	transfection	transfection	NN
cord-014597-66vd2mdu	2390	45	agents	agent	NNS
cord-014597-66vd2mdu	2390	46	for	for	IN
cord-014597-66vd2mdu	2390	47	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2390	48	cells	cell	NNS
cord-014597-66vd2mdu	2390	49	Systematic	systematic	JJ
cord-014597-66vd2mdu	2390	50	Study	Study	NNP
cord-014597-66vd2mdu	2390	51	of	of	IN
cord-014597-66vd2mdu	2390	52	a	a	DT
cord-014597-66vd2mdu	2390	53	Library	Library	NNP
cord-014597-66vd2mdu	2390	54	of	of	IN
cord-014597-66vd2mdu	2390	55	PDMAEMA	PDMAEMA	NNP
cord-014597-66vd2mdu	2390	56	-	-	HYPH
cord-014597-66vd2mdu	2390	57	Based	Based	NNP
cord-014597-66vd2mdu	2390	58	,	,	,
cord-014597-66vd2mdu	2390	59	Superparamagnetic	Superparamagnetic	NNP
cord-014597-66vd2mdu	2390	60	Nano	Nano	NNP
cord-014597-66vd2mdu	2390	61	-	-	HYPH
cord-014597-66vd2mdu	2390	62	Stars	Stars	NNP
cord-014597-66vd2mdu	2390	63	for	for	IN
cord-014597-66vd2mdu	2390	64	the	the	DT
cord-014597-66vd2mdu	2390	65	Transfection	Transfection	NNP
cord-014597-66vd2mdu	2390	66	of	of	IN
cord-014597-66vd2mdu	2390	67	CHO	CHO	NNP
cord-014597-66vd2mdu	2390	68	-	-	HYPH
cord-014597-66vd2mdu	2390	69	K1	K1	NNP
cord-014597-66vd2mdu	2390	70	Cells	Cells	NNPS
cord-014597-66vd2mdu	2390	71	Systems	Systems	NNPS
cord-014597-66vd2mdu	2390	72	biology	biology	NN
cord-014597-66vd2mdu	2390	73	of	of	IN
cord-014597-66vd2mdu	2390	74	unfolded	unfold	VBN
cord-014597-66vd2mdu	2390	75	protein	protein	NN
cord-014597-66vd2mdu	2390	76	response	response	NN
cord-014597-66vd2mdu	2390	77	in	in	IN
cord-014597-66vd2mdu	2390	78	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2390	79	CHO	CHO	NNP
cord-014597-66vd2mdu	2390	80	cells	cell	NNS
cord-014597-66vd2mdu	2390	81	Dynamics	dynamic	NNS
cord-014597-66vd2mdu	2390	82	of	of	IN
cord-014597-66vd2mdu	2390	83	unfolded	unfold	VBN
cord-014597-66vd2mdu	2390	84	protein	protein	NN
cord-014597-66vd2mdu	2390	85	response	response	NN
cord-014597-66vd2mdu	2390	86	in	in	IN
cord-014597-66vd2mdu	2390	87	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2390	88	CHO	CHO	NNP
cord-014597-66vd2mdu	2390	89	cells	cell	NNS
cord-014597-66vd2mdu	2390	90	Cells	cell	NNS
cord-014597-66vd2mdu	2390	91	were	be	VBD
cord-014597-66vd2mdu	2390	92	transfected	transfecte	VBN
cord-014597-66vd2mdu	2390	93	with	with	IN
cord-014597-66vd2mdu	2390	94	NP@(PDMAEMA	NP@(PDMAEMA	NNP
cord-014597-66vd2mdu	2390	95	1037	1037	CD
cord-014597-66vd2mdu	2390	96	)	)	-RRB-
cord-014597-66vd2mdu	2391	1	46	46	CD
cord-014597-66vd2mdu	2392	1	(	(	-LRB-
cord-014597-66vd2mdu	2392	2	N	N	NNP
cord-014597-66vd2mdu	2392	3	/	/	SYM
cord-014597-66vd2mdu	2392	4	P	P	NNP
cord-014597-66vd2mdu	2392	5	15	15	CD
cord-014597-66vd2mdu	2392	6	)	)	-RRB-
cord-014597-66vd2mdu	2392	7	,	,	,
cord-014597-66vd2mdu	2392	8	separated	separate	VBD
cord-014597-66vd2mdu	2392	9	24	24	CD
cord-014597-66vd2mdu	2392	10	h	h	NN
cord-014597-66vd2mdu	2392	11	post	post	NN
cord-014597-66vd2mdu	2392	12	transfection	transfection	NN
cord-014597-66vd2mdu	2392	13	(	(	-LRB-
cord-014597-66vd2mdu	2392	14	t	t	NN
cord-014597-66vd2mdu	2392	15	=	=	SYM
cord-014597-66vd2mdu	2392	16	0	0	NFP
cord-014597-66vd2mdu	2392	17	)	)	-RRB-
cord-014597-66vd2mdu	2392	18	by	by	IN
cord-014597-66vd2mdu	2392	19	magnetically	magnetically	RB
cord-014597-66vd2mdu	2392	20	-	-	HYPH
cord-014597-66vd2mdu	2392	21	assisted	assist	VBN
cord-014597-66vd2mdu	2392	22	cell	cell	NN
cord-014597-66vd2mdu	2392	23	sorting	sorting	NN
cord-014597-66vd2mdu	2392	24	and	and	CC
cord-014597-66vd2mdu	2392	25	placed	place	VBN
cord-014597-66vd2mdu	2392	26	into	into	IN
cord-014597-66vd2mdu	2392	27	separated	separated	JJ
cord-014597-66vd2mdu	2392	28	cultures	culture	NNS
cord-014597-66vd2mdu	2392	29	.	.	.
cord-014597-66vd2mdu	2393	1	The	the	DT
cord-014597-66vd2mdu	2393	2	bars	bar	NNS
cord-014597-66vd2mdu	2393	3	represent	represent	VBP
cord-014597-66vd2mdu	2393	4	the	the	DT
cord-014597-66vd2mdu	2393	5	overall	overall	JJ
cord-014597-66vd2mdu	2393	6	transfection	transfection	NN
cord-014597-66vd2mdu	2393	7	efficiency	efficiency	NN
cord-014597-66vd2mdu	2393	8	.	.	.
cord-014597-66vd2mdu	2394	1	Distribution	distribution	NN
cord-014597-66vd2mdu	2394	2	of	of	IN
cord-014597-66vd2mdu	2394	3	low	low	JJ
cord-014597-66vd2mdu	2394	4	(	(	-LRB-
cord-014597-66vd2mdu	2394	5	light	light	JJ
cord-014597-66vd2mdu	2394	6	green	green	JJ
cord-014597-66vd2mdu	2394	7	)	)	-RRB-
cord-014597-66vd2mdu	2394	8	,	,	,
cord-014597-66vd2mdu	2394	9	middle	middle	JJ
cord-014597-66vd2mdu	2394	10	(	(	-LRB-
cord-014597-66vd2mdu	2394	11	green	green	JJ
cord-014597-66vd2mdu	2394	12	)	)	-RRB-
cord-014597-66vd2mdu	2394	13	,	,	,
cord-014597-66vd2mdu	2394	14	and	and	CC
cord-014597-66vd2mdu	2394	15	high	high	JJ
cord-014597-66vd2mdu	2394	16	(	(	-LRB-
cord-014597-66vd2mdu	2394	17	dark	dark	JJ
cord-014597-66vd2mdu	2394	18	green	green	JJ
cord-014597-66vd2mdu	2394	19	)	)	-RRB-
cord-014597-66vd2mdu	2394	20	producers	producer	NNS
cord-014597-66vd2mdu	2394	21	within	within	IN
cord-014597-66vd2mdu	2394	22	the	the	DT
cord-014597-66vd2mdu	2394	23	EGFP	EGFP	NNP
cord-014597-66vd2mdu	2394	24	-	-	HYPH
cord-014597-66vd2mdu	2394	25	expressing	express	VBG
cord-014597-66vd2mdu	2394	26	cell	cell	NN
cord-014597-66vd2mdu	2394	27	fraction	fraction	NN
cord-014597-66vd2mdu	2394	28	.	.	.
cord-014597-66vd2mdu	2395	1	Data	datum	NNS
cord-014597-66vd2mdu	2395	2	represent	represent	VBP
cord-014597-66vd2mdu	2395	3	one	one	CD
cord-014597-66vd2mdu	2395	4	experiment	experiment	NN
cord-014597-66vd2mdu	2395	5	carried	carry	VBN
cord-014597-66vd2mdu	2395	6	out	out	RP
cord-014597-66vd2mdu	2395	7	in	in	IN
cord-014597-66vd2mdu	2395	8	duplicate	duplicate	NN
cord-014597-66vd2mdu	2395	9	,	,	,
cord-014597-66vd2mdu	2395	10	with	with	IN
cord-014597-66vd2mdu	2395	11	random	random	JJ
cord-014597-66vd2mdu	2395	12	experimental	experimental	JJ
cord-014597-66vd2mdu	2395	13	error	error	NN
cord-014597-66vd2mdu	2395	14	shown	show	VBN
cord-014597-66vd2mdu	2395	15	.	.	.
cord-014597-66vd2mdu	2396	1	d	d	LS
cord-014597-66vd2mdu	2396	2	Correlogram	Correlogram	NNP
cord-014597-66vd2mdu	2396	3	between	between	IN
cord-014597-66vd2mdu	2396	4	the	the	DT
cord-014597-66vd2mdu	2396	5	molecular	molecular	JJ
cord-014597-66vd2mdu	2396	6	characteristics	characteristic	NNS
cord-014597-66vd2mdu	2396	7	of	of	IN
cord-014597-66vd2mdu	2396	8	the	the	DT
cord-014597-66vd2mdu	2396	9	nano	nano	NN
cord-014597-66vd2mdu	2396	10	-	-	HYPH
cord-014597-66vd2mdu	2396	11	stars	star	NNS
cord-014597-66vd2mdu	2396	12	(	(	-LRB-
cord-014597-66vd2mdu	2396	13	core	core	NN
cord-014597-66vd2mdu	2396	14	diameter	diameter	NN
cord-014597-66vd2mdu	2396	15	,	,	,
cord-014597-66vd2mdu	2396	16	arm	arm	NN
cord-014597-66vd2mdu	2396	17	density	density	NN
cord-014597-66vd2mdu	2396	18	,	,	,
cord-014597-66vd2mdu	2396	19	arm	arm	NN
cord-014597-66vd2mdu	2396	20	length	length	NN
cord-014597-66vd2mdu	2396	21	,	,	,
cord-014597-66vd2mdu	2396	22	number	number	NN
cord-014597-66vd2mdu	2396	23	of	of	IN
cord-014597-66vd2mdu	2396	24	monomeric	monomeric	JJ
cord-014597-66vd2mdu	2396	25	units	unit	NNS
cord-014597-66vd2mdu	2396	26	per	per	IN
cord-014597-66vd2mdu	2396	27	nano	nano	JJ
cord-014597-66vd2mdu	2396	28	-	-	HYPH
cord-014597-66vd2mdu	2396	29	star	star	NN
cord-014597-66vd2mdu	2396	30	)	)	-RRB-
cord-014597-66vd2mdu	2396	31	,	,	,
cord-014597-66vd2mdu	2396	32	the	the	DT
cord-014597-66vd2mdu	2396	33	physicochemical	physicochemical	JJ
cord-014597-66vd2mdu	2396	34	properties	property	NNS
cord-014597-66vd2mdu	2396	35	of	of	IN
cord-014597-66vd2mdu	2396	36	the	the	DT
cord-014597-66vd2mdu	2396	37	corresponding	correspond	VBG
cord-014597-66vd2mdu	2396	38	polyplexes	polyplexes	JJ
cord-014597-66vd2mdu	2396	39	(	(	-LRB-
cord-014597-66vd2mdu	2396	40	hydrodynamic	hydrodynamic	JJ
cord-014597-66vd2mdu	2396	41	radius	radius	NN
cord-014597-66vd2mdu	2396	42	,	,	,
cord-014597-66vd2mdu	2396	43	zeta	zeta	NNP
cord-014597-66vd2mdu	2396	44	potential	potential	NN
cord-014597-66vd2mdu	2396	45	)	)	-RRB-
cord-014597-66vd2mdu	2396	46	,	,	,
cord-014597-66vd2mdu	2396	47	the	the	DT
cord-014597-66vd2mdu	2396	48	transfection	transfection	NN
cord-014597-66vd2mdu	2396	49	conditions	condition	NNS
cord-014597-66vd2mdu	2396	50	(	(	-LRB-
cord-014597-66vd2mdu	2396	51	N	N	NNP
cord-014597-66vd2mdu	2396	52	/	/	SYM
cord-014597-66vd2mdu	2396	53	P	p	NN
cord-014597-66vd2mdu	2396	54	ratio	ratio	NN
cord-014597-66vd2mdu	2396	55	,	,	,
cord-014597-66vd2mdu	2396	56	amount	amount	NN
cord-014597-66vd2mdu	2396	57	of	of	IN
cord-014597-66vd2mdu	2396	58	polymer	polymer	NN
cord-014597-66vd2mdu	2396	59	)	)	-RRB-
cord-014597-66vd2mdu	2396	60	,	,	,
cord-014597-66vd2mdu	2396	61	and	and	CC
cord-014597-66vd2mdu	2396	62	the	the	DT
cord-014597-66vd2mdu	2396	63	cellular	cellular	JJ
cord-014597-66vd2mdu	2396	64	reactions	reaction	NNS
cord-014597-66vd2mdu	2396	65	(	(	-LRB-
cord-014597-66vd2mdu	2396	66	transfection	transfection	NN
cord-014597-66vd2mdu	2396	67	efficiency	efficiency	NN
cord-014597-66vd2mdu	2396	68	,	,	,
cord-014597-66vd2mdu	2396	69	magnetism	magnetism	NN
cord-014597-66vd2mdu	2396	70	,	,	.
cord-014597-66vd2mdu	2397	1	viability	viability	NN
cord-014597-66vd2mdu	2397	2	)	)	-RRB-
cord-014597-66vd2mdu	2397	3	Production	production	NN
cord-014597-66vd2mdu	2397	4	of	of	IN
cord-014597-66vd2mdu	2397	5	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2397	6	protein	protein	NN
cord-014597-66vd2mdu	2397	7	therapeutics	therapeutic	NNS
cord-014597-66vd2mdu	2397	8	in	in	IN
cord-014597-66vd2mdu	2397	9	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2397	10	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2397	11	cells	cell	NNS
cord-014597-66vd2mdu	2397	12	Position	position	VBP
cord-014597-66vd2mdu	2397	13	effects	effect	NNS
cord-014597-66vd2mdu	2397	14	on	on	IN
cord-014597-66vd2mdu	2397	15	eukaryotic	eukaryotic	JJ
cord-014597-66vd2mdu	2397	16	gene	gene	NN
cord-014597-66vd2mdu	2397	17	expression	expression	NN
cord-014597-66vd2mdu	2397	18	Bacterial	bacterial	JJ
cord-014597-66vd2mdu	2397	19	artificial	artificial	JJ
cord-014597-66vd2mdu	2397	20	chromosome	chromosome	NN
cord-014597-66vd2mdu	2397	21	library	library	NN
cord-014597-66vd2mdu	2397	22	for	for	IN
cord-014597-66vd2mdu	2397	23	genome	genome	NN
cord-014597-66vd2mdu	2397	24	-	-	HYPH
cord-014597-66vd2mdu	2397	25	wide	wide	JJ
cord-014597-66vd2mdu	2397	26	analysis	analysis	NN
cord-014597-66vd2mdu	2397	27	of	of	IN
cord-014597-66vd2mdu	2397	28	Chinese	chinese	JJ
cord-014597-66vd2mdu	2397	29	hamster	hamster	NN
cord-014597-66vd2mdu	2397	30	ovary	ovary	NN
cord-014597-66vd2mdu	2397	31	cells	cell	NNS
cord-014597-66vd2mdu	2397	32	Construction	Construction	NNP
cord-014597-66vd2mdu	2397	33	of	of	IN
cord-014597-66vd2mdu	2397	34	BAC	BAC	NNP
cord-014597-66vd2mdu	2397	35	-	-	HYPH
cord-014597-66vd2mdu	2397	36	based	base	VBN
cord-014597-66vd2mdu	2397	37	physical	physical	JJ
cord-014597-66vd2mdu	2397	38	map	map	NN
cord-014597-66vd2mdu	2397	39	and	and	CC
cord-014597-66vd2mdu	2397	40	analysis	analysis	NN
cord-014597-66vd2mdu	2397	41	of	of	IN
cord-014597-66vd2mdu	2397	42	chromosome	chromosome	NN
cord-014597-66vd2mdu	2397	43	rearrangement	rearrangement	NN
cord-014597-66vd2mdu	2397	44	in	in	IN
cord-014597-66vd2mdu	2397	45	Chinese	chinese	JJ
cord-014597-66vd2mdu	2397	46	hamster	hamster	NN
cord-014597-66vd2mdu	2397	47	ovary	ovary	NN
cord-014597-66vd2mdu	2397	48	cell	cell	NN
cord-014597-66vd2mdu	2397	49	lines	line	NNS
cord-014597-66vd2mdu	2397	50	Suguru	Suguru	NNP
cord-014597-66vd2mdu	2397	51	Imanishi	Imanishi	NNP
cord-014597-66vd2mdu	2397	52	1	1	CD
cord-014597-66vd2mdu	2397	53	,	,	,
cord-014597-66vd2mdu	2397	54	Akira	Akira	NNP
cord-014597-66vd2mdu	2397	55	Ito	Ito	NNP
cord-014597-66vd2mdu	2397	56	1	1	CD
cord-014597-66vd2mdu	2397	57	,	,	,
cord-014597-66vd2mdu	2397	58	Masamichi	Masamichi	NNP
cord-014597-66vd2mdu	2397	59	Kamihira	Kamihira	NNP
cord-014597-66vd2mdu	2398	1	1,2	1,2	CD
cord-014597-66vd2mdu	2398	2	1	1	CD
cord-014597-66vd2mdu	2398	3	Department	Department	NNP
cord-014597-66vd2mdu	2398	4	of	of	IN
cord-014597-66vd2mdu	2398	5	Chemical	Chemical	NNP
cord-014597-66vd2mdu	2398	6	Engineering	Engineering	NNP
cord-014597-66vd2mdu	2398	7	Cre	Cre	NNP
cord-014597-66vd2mdu	2398	8	recombinase	recombinase	NN
cord-014597-66vd2mdu	2398	9	-	-	HYPH
cord-014597-66vd2mdu	2398	10	mediated	mediate	VBN
cord-014597-66vd2mdu	2398	11	sitespecific	sitespecific	NN
cord-014597-66vd2mdu	2398	12	modification	modification	NN
cord-014597-66vd2mdu	2398	13	of	of	IN
cord-014597-66vd2mdu	2398	14	a	a	DT
cord-014597-66vd2mdu	2398	15	cellular	cellular	JJ
cord-014597-66vd2mdu	2398	16	genome	genome	NN
cord-014597-66vd2mdu	2398	17	using	use	VBG
cord-014597-66vd2mdu	2398	18	an	an	DT
cord-014597-66vd2mdu	2398	19	integrasedefective	integrasedefective	JJ
cord-014597-66vd2mdu	2398	20	retroviral	retroviral	NN
cord-014597-66vd2mdu	2398	21	vector	vector	NN
cord-014597-66vd2mdu	2398	22	Targeted	target	VBN
cord-014597-66vd2mdu	2398	23	transgene	transgene	NN
cord-014597-66vd2mdu	2398	24	insertion	insertion	NN
cord-014597-66vd2mdu	2398	25	into	into	IN
cord-014597-66vd2mdu	2398	26	the	the	DT
cord-014597-66vd2mdu	2398	27	CHO	CHO	NNP
cord-014597-66vd2mdu	2398	28	cell	cell	NN
cord-014597-66vd2mdu	2398	29	genome	genome	NN
cord-014597-66vd2mdu	2398	30	using	use	VBG
cord-014597-66vd2mdu	2398	31	Cre	Cre	NNP
cord-014597-66vd2mdu	2398	32	recombinase	recombinase	NN
cord-014597-66vd2mdu	2398	33	-	-	HYPH
cord-014597-66vd2mdu	2398	34	incorporating	incorporate	VBG
cord-014597-66vd2mdu	2398	35	integrase	integrase	JJ
cord-014597-66vd2mdu	2398	36	-	-	HYPH
cord-014597-66vd2mdu	2398	37	defective	defective	JJ
cord-014597-66vd2mdu	2398	38	retroviral	retroviral	NN
cord-014597-66vd2mdu	2398	39	vectors	vector	NNS
cord-014597-66vd2mdu	2398	40	An	an	DT
cord-014597-66vd2mdu	2398	41	accumulative	accumulative	JJ
cord-014597-66vd2mdu	2398	42	site	site	NN
cord-014597-66vd2mdu	2398	43	-	-	HYPH
cord-014597-66vd2mdu	2398	44	specific	specific	JJ
cord-014597-66vd2mdu	2398	45	gene	gene	NN
cord-014597-66vd2mdu	2398	46	integration	integration	NN
cord-014597-66vd2mdu	2398	47	system	system	NN
cord-014597-66vd2mdu	2398	48	using	use	VBG
cord-014597-66vd2mdu	2398	49	Cre	Cre	NNP
cord-014597-66vd2mdu	2398	50	recombinase	recombinase	NN
cord-014597-66vd2mdu	2398	51	-	-	HYPH
cord-014597-66vd2mdu	2398	52	mediated	mediate	VBN
cord-014597-66vd2mdu	2398	53	cassette	cassette	NN
cord-014597-66vd2mdu	2398	54	exchange	exchange	NN
cord-014597-66vd2mdu	2398	55	Improved	improve	VBN
cord-014597-66vd2mdu	2398	56	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2398	57	antibody	antibody	NN
cord-014597-66vd2mdu	2398	58	production	production	NN
cord-014597-66vd2mdu	2398	59	by	by	IN
cord-014597-66vd2mdu	2398	60	CHO	CHO	NNP
cord-014597-66vd2mdu	2398	61	cells	cell	NNS
cord-014597-66vd2mdu	2398	62	using	use	VBG
cord-014597-66vd2mdu	2398	63	a	a	DT
cord-014597-66vd2mdu	2398	64	production	production	NN
cord-014597-66vd2mdu	2398	65	enhancer	enhancer	NN
cord-014597-66vd2mdu	2398	66	DNA	dna	NN
cord-014597-66vd2mdu	2398	67	element	element	NN
cord-014597-66vd2mdu	2398	68	with	with	IN
cord-014597-66vd2mdu	2398	69	repeated	repeat	VBN
cord-014597-66vd2mdu	2398	70	transgene	transgene	NN
cord-014597-66vd2mdu	2398	71	integration	integration	NN
cord-014597-66vd2mdu	2398	72	at	at	IN
cord-014597-66vd2mdu	2398	73	a	a	DT
cord-014597-66vd2mdu	2398	74	predetermined	predetermine	VBN
cord-014597-66vd2mdu	2398	75	chromosomal	chromosomal	JJ
cord-014597-66vd2mdu	2398	76	site	site	NN
cord-014597-66vd2mdu	2398	77	Lipid	lipid	NN
cord-014597-66vd2mdu	2398	78	extraction	extraction	NN
cord-014597-66vd2mdu	2398	79	by	by	IN
cord-014597-66vd2mdu	2398	80	methyl	methyl	NN
cord-014597-66vd2mdu	2398	81	-	-	HYPH
cord-014597-66vd2mdu	2398	82	tert	tert	NN
cord-014597-66vd2mdu	2398	83	-	-	HYPH
cord-014597-66vd2mdu	2398	84	butyl	butyl	NN
cord-014597-66vd2mdu	2398	85	ether	ether	NN
cord-014597-66vd2mdu	2398	86	for	for	IN
cord-014597-66vd2mdu	2398	87	high	high	JJ
cord-014597-66vd2mdu	2398	88	-	-	HYPH
cord-014597-66vd2mdu	2398	89	throughput	throughput	NN
cord-014597-66vd2mdu	2398	90	lipidomics	lipidomic	NNS
cord-014597-66vd2mdu	2399	1	A	a	DT
cord-014597-66vd2mdu	2399	2	simple	simple	JJ
cord-014597-66vd2mdu	2399	3	method	method	NN
cord-014597-66vd2mdu	2399	4	for	for	IN
cord-014597-66vd2mdu	2399	5	the	the	DT
cord-014597-66vd2mdu	2399	6	isolation	isolation	NN
cord-014597-66vd2mdu	2399	7	and	and	CC
cord-014597-66vd2mdu	2399	8	purification	purification	NN
cord-014597-66vd2mdu	2399	9	of	of	IN
cord-014597-66vd2mdu	2399	10	total	total	JJ
cord-014597-66vd2mdu	2399	11	lipids	lipid	NNS
cord-014597-66vd2mdu	2399	12	from	from	IN
cord-014597-66vd2mdu	2399	13	animal	animal	NN
cord-014597-66vd2mdu	2399	14	tissues	tissue	NNS
cord-014597-66vd2mdu	2399	15	Using	use	VBG
cord-014597-66vd2mdu	2399	16	Baculovirus	Baculovirus	NNP
cord-014597-66vd2mdu	2399	17	as	as	IN
cord-014597-66vd2mdu	2399	18	a	a	DT
cord-014597-66vd2mdu	2399	19	gene	gene	NN
cord-014597-66vd2mdu	2399	20	shuttle	shuttle	NN
cord-014597-66vd2mdu	2399	21	in	in	IN
cord-014597-66vd2mdu	2399	22	hMSC	hmsc	NN
cord-014597-66vd2mdu	2399	23	:	:	:
cord-014597-66vd2mdu	2399	24	optimization	optimization	NN
cord-014597-66vd2mdu	2399	25	of	of	IN
cord-014597-66vd2mdu	2399	26	transduction	transduction	NN
cord-014597-66vd2mdu	2399	27	efficacy	efficacy	NN
cord-014597-66vd2mdu	2399	28	Gundula	Gundula	NNP
cord-014597-66vd2mdu	2399	29	Sprick	Sprick	NNP
cord-014597-66vd2mdu	2399	30	1	1	CD
cord-014597-66vd2mdu	2399	31	Clinical	clinical	JJ
cord-014597-66vd2mdu	2399	32	applications	application	NNS
cord-014597-66vd2mdu	2399	33	of	of	IN
cord-014597-66vd2mdu	2399	34	mesenchymal	mesenchymal	JJ
cord-014597-66vd2mdu	2399	35	stem	stem	NN
cord-014597-66vd2mdu	2399	36	cells	cell	NNS
cord-014597-66vd2mdu	2400	1	Concise	concise	JJ
cord-014597-66vd2mdu	2400	2	review	review	NN
cord-014597-66vd2mdu	2400	3	:	:	:
cord-014597-66vd2mdu	2401	1	Mesenchymal	Mesenchymal	NNS
cord-014597-66vd2mdu	2401	2	stem	stem	VBP
cord-014597-66vd2mdu	2401	3	cell	cell	NN
cord-014597-66vd2mdu	2401	4	treatment	treatment	NN
cord-014597-66vd2mdu	2401	5	of	of	IN
cord-014597-66vd2mdu	2401	6	the	the	DT
cord-014597-66vd2mdu	2401	7	complications	complication	NNS
cord-014597-66vd2mdu	2401	8	of	of	IN
cord-014597-66vd2mdu	2401	9	diabetes	diabetes	NN
cord-014597-66vd2mdu	2401	10	mellitus	mellitus	NN
cord-014597-66vd2mdu	2401	11	Wharton	Wharton	NNP
cord-014597-66vd2mdu	2401	12	's	's	POS
cord-014597-66vd2mdu	2401	13	Jelly	Jelly	NNP
cord-014597-66vd2mdu	2401	14	-	-	HYPH
cord-014597-66vd2mdu	2401	15	Derived	derive	VBN
cord-014597-66vd2mdu	2401	16	Mesenchymal	Mesenchymal	NNP
cord-014597-66vd2mdu	2401	17	Stromal	Stromal	NNP
cord-014597-66vd2mdu	2401	18	Cells	Cells	NNP
cord-014597-66vd2mdu	2401	19	as	as	IN
cord-014597-66vd2mdu	2401	20	a	a	DT
cord-014597-66vd2mdu	2401	21	Promising	promise	VBG
cord-014597-66vd2mdu	2401	22	Cellular	Cellular	NNP
cord-014597-66vd2mdu	2401	23	Therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2401	24	Strategy	Strategy	NNP
cord-014597-66vd2mdu	2401	25	for	for	IN
cord-014597-66vd2mdu	2401	26	the	the	DT
cord-014597-66vd2mdu	2401	27	Management	Management	NNP
cord-014597-66vd2mdu	2401	28	of	of	IN
cord-014597-66vd2mdu	2401	29	Graft	graft	NN
cord-014597-66vd2mdu	2401	30	-	-	HYPH
cord-014597-66vd2mdu	2401	31	versus	versus	IN
cord-014597-66vd2mdu	2401	32	-	-	HYPH
cord-014597-66vd2mdu	2401	33	Host	Host	NNP
cord-014597-66vd2mdu	2401	34	Disease	Disease	NNP
cord-014597-66vd2mdu	2401	35	Gene	Gene	NNP
cord-014597-66vd2mdu	2401	36	therapy	therapy	NN
cord-014597-66vd2mdu	2401	37	:	:	:
cord-014597-66vd2mdu	2401	38	twenty	twenty	CD
cord-014597-66vd2mdu	2401	39	-	-	HYPH
cord-014597-66vd2mdu	2401	40	first	first	JJ
cord-014597-66vd2mdu	2401	41	century	century	NN
cord-014597-66vd2mdu	2401	42	medicine	medicine	NN
cord-014597-66vd2mdu	2401	43	State	state	NN
cord-014597-66vd2mdu	2401	44	-	-	HYPH
cord-014597-66vd2mdu	2401	45	of	of	IN
cord-014597-66vd2mdu	2401	46	-	-	HYPH
cord-014597-66vd2mdu	2401	47	the	the	DT
cord-014597-66vd2mdu	2401	48	-	-	HYPH
cord-014597-66vd2mdu	2401	49	art	art	NN
cord-014597-66vd2mdu	2401	50	gene	gene	NN
cord-014597-66vd2mdu	2401	51	-	-	HYPH
cord-014597-66vd2mdu	2401	52	based	base	VBN
cord-014597-66vd2mdu	2401	53	therapies	therapy	NNS
cord-014597-66vd2mdu	2401	54	:	:	:
cord-014597-66vd2mdu	2402	1	the	the	DT
cord-014597-66vd2mdu	2402	2	road	road	NN
cord-014597-66vd2mdu	2402	3	ahead	ahead	RB
cord-014597-66vd2mdu	2402	4	Viral	viral	JJ
cord-014597-66vd2mdu	2402	5	vectors	vector	NNS
cord-014597-66vd2mdu	2402	6	:	:	:
cord-014597-66vd2mdu	2402	7	a	a	DT
cord-014597-66vd2mdu	2402	8	look	look	NN
cord-014597-66vd2mdu	2402	9	back	back	RB
cord-014597-66vd2mdu	2402	10	and	and	CC
cord-014597-66vd2mdu	2402	11	ahead	ahead	RB
cord-014597-66vd2mdu	2402	12	on	on	IN
cord-014597-66vd2mdu	2402	13	gene	gene	NN
cord-014597-66vd2mdu	2402	14	transfer	transfer	NN
cord-014597-66vd2mdu	2402	15	technology	technology	NN
cord-014597-66vd2mdu	2402	16	Basic	basic	JJ
cord-014597-66vd2mdu	2402	17	Biology	Biology	NNP
cord-014597-66vd2mdu	2402	18	of	of	IN
cord-014597-66vd2mdu	2402	19	Adeno	Adeno	NNP
cord-014597-66vd2mdu	2402	20	-	-	HYPH
cord-014597-66vd2mdu	2402	21	Associated	Associated	NNP
cord-014597-66vd2mdu	2402	22	Virus	Virus	NNP
cord-014597-66vd2mdu	2402	23	(	(	-LRB-
cord-014597-66vd2mdu	2402	24	AAV	AAV	NNP
cord-014597-66vd2mdu	2402	25	)	)	-RRB-
cord-014597-66vd2mdu	2402	26	Vectors	Vectors	NNP
cord-014597-66vd2mdu	2402	27	Used	use	VBN
cord-014597-66vd2mdu	2402	28	in	in	IN
cord-014597-66vd2mdu	2402	29	Gene	Gene	NNP
cord-014597-66vd2mdu	2402	30	Therapy	Therapy	NNP
cord-014597-66vd2mdu	2402	31	Biosafety	Biosafety	NNP
cord-014597-66vd2mdu	2402	32	challenges	challenge	VBZ
cord-014597-66vd2mdu	2402	33	for	for	IN
cord-014597-66vd2mdu	2402	34	use	use	NN
cord-014597-66vd2mdu	2402	35	of	of	IN
cord-014597-66vd2mdu	2402	36	lentiviral	lentiviral	JJ
cord-014597-66vd2mdu	2402	37	vectors	vector	NNS
cord-014597-66vd2mdu	2402	38	in	in	IN
cord-014597-66vd2mdu	2402	39	gene	gene	NN
cord-014597-66vd2mdu	2402	40	therapy	therapy	NN
cord-014597-66vd2mdu	2402	41	Adenoviral	Adenoviral	NNP
cord-014597-66vd2mdu	2402	42	vector	vector	NN
cord-014597-66vd2mdu	2402	43	-	-	HYPH
cord-014597-66vd2mdu	2402	44	mediated	mediate	VBN
cord-014597-66vd2mdu	2402	45	gene	gene	NN
cord-014597-66vd2mdu	2402	46	therapy	therapy	NN
cord-014597-66vd2mdu	2402	47	for	for	IN
cord-014597-66vd2mdu	2402	48	gliomas	glioma	NNS
cord-014597-66vd2mdu	2402	49	:	:	:
cord-014597-66vd2mdu	2403	1	coming	come	VBG
cord-014597-66vd2mdu	2403	2	of	of	IN
cord-014597-66vd2mdu	2403	3	age	age	NN
cord-014597-66vd2mdu	2403	4	Manufacturing	manufacturing	NN
cord-014597-66vd2mdu	2403	5	of	of	IN
cord-014597-66vd2mdu	2403	6	viral	viral	JJ
cord-014597-66vd2mdu	2403	7	vectors	vector	NNS
cord-014597-66vd2mdu	2403	8	for	for	IN
cord-014597-66vd2mdu	2403	9	gene	gene	NN
cord-014597-66vd2mdu	2403	10	therapy	therapy	NN
cord-014597-66vd2mdu	2403	11	:	:	:
cord-014597-66vd2mdu	2404	1	part	part	NN
cord-014597-66vd2mdu	2404	2	I.	I.	NNP
cord-014597-66vd2mdu	2404	3	Upstream	Upstream	NNP
cord-014597-66vd2mdu	2404	4	processing	process	VBG
cord-014597-66vd2mdu	2404	5	The	the	DT
cord-014597-66vd2mdu	2404	6	complete	complete	JJ
cord-014597-66vd2mdu	2404	7	DNA	dna	NN
cord-014597-66vd2mdu	2404	8	sequence	sequence	NN
cord-014597-66vd2mdu	2404	9	of	of	IN
cord-014597-66vd2mdu	2404	10	Autographa	Autographa	NNP
cord-014597-66vd2mdu	2404	11	californica	californica	NNP
cord-014597-66vd2mdu	2404	12	nuclear	nuclear	JJ
cord-014597-66vd2mdu	2404	13	polyhedrosis	polyhedrosis	NN
cord-014597-66vd2mdu	2404	14	virus	virus	NN
cord-014597-66vd2mdu	2404	15	Efficient	efficient	JJ
cord-014597-66vd2mdu	2404	16	gene	gene	NN
cord-014597-66vd2mdu	2404	17	transfer	transfer	NN
cord-014597-66vd2mdu	2404	18	into	into	IN
cord-014597-66vd2mdu	2404	19	human	human	JJ
cord-014597-66vd2mdu	2404	20	hepatocytes	hepatocyte	NNS
cord-014597-66vd2mdu	2404	21	by	by	IN
cord-014597-66vd2mdu	2404	22	baculovirus	baculovirus	NN
cord-014597-66vd2mdu	2404	23	vectors	vector	NNS
cord-014597-66vd2mdu	2404	24	Efficient	Efficient	NNP
cord-014597-66vd2mdu	2404	25	Transduction	Transduction	NNP
cord-014597-66vd2mdu	2404	26	of	of	IN
cord-014597-66vd2mdu	2404	27	Mammalian	mammalian	JJ
cord-014597-66vd2mdu	2404	28	Cells	Cells	NNPS
cord-014597-66vd2mdu	2404	29	by	by	IN
cord-014597-66vd2mdu	2404	30	a	a	DT
cord-014597-66vd2mdu	2404	31	Recombinant	Recombinant	NNP
cord-014597-66vd2mdu	2404	32	Baculovirus	Baculovirus	NNP
cord-014597-66vd2mdu	2404	33	Having	have	VBG
cord-014597-66vd2mdu	2404	34	the	the	DT
cord-014597-66vd2mdu	2404	35	Vesicular	Vesicular	NNP
cord-014597-66vd2mdu	2404	36	Stomatitis	Stomatitis	NNP
cord-014597-66vd2mdu	2404	37	Virus	Virus	NNP
cord-014597-66vd2mdu	2404	38	G	g	NN
cord-014597-66vd2mdu	2404	39	Glycoprotein	glycoprotein	NN
cord-014597-66vd2mdu	2404	40	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	2404	41	baculoviruses	baculoviruse	NNS
cord-014597-66vd2mdu	2404	42	as	as	IN
cord-014597-66vd2mdu	2404	43	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2404	44	cell	cell	NN
cord-014597-66vd2mdu	2404	45	gene	gene	NN
cord-014597-66vd2mdu	2404	46	-	-	HYPH
cord-014597-66vd2mdu	2404	47	delivery	delivery	NN
cord-014597-66vd2mdu	2404	48	vectors	vector	NNS
cord-014597-66vd2mdu	2405	1	Post	post	JJ
cord-014597-66vd2mdu	2405	2	-	-	JJ
cord-014597-66vd2mdu	2405	3	transcriptional	transcriptional	JJ
cord-014597-66vd2mdu	2405	4	regulatory	regulatory	JJ
cord-014597-66vd2mdu	2405	5	element	element	NN
cord-014597-66vd2mdu	2405	6	boosts	boost	VBZ
cord-014597-66vd2mdu	2405	7	baculovirusmediated	baculovirusmediated	JJ
cord-014597-66vd2mdu	2405	8	gene	gene	NN
cord-014597-66vd2mdu	2405	9	expression	expression	NN
cord-014597-66vd2mdu	2405	10	in	in	IN
cord-014597-66vd2mdu	2405	11	vertebrate	vertebrate	JJ
cord-014597-66vd2mdu	2405	12	cells	cell	NNS
cord-014597-66vd2mdu	2406	1	Baculoviral	Baculoviral	NNP
cord-014597-66vd2mdu	2406	2	Vector	Vector	NNP
cord-014597-66vd2mdu	2406	3	-	-	HYPH
cord-014597-66vd2mdu	2406	4	Mediated	mediate	VBN
cord-014597-66vd2mdu	2406	5	Transient	Transient	NNP
cord-014597-66vd2mdu	2406	6	and	and	CC
cord-014597-66vd2mdu	2406	7	Stable	stable	JJ
cord-014597-66vd2mdu	2406	8	Transgene	transgene	NN
cord-014597-66vd2mdu	2406	9	Expression	expression	NN
cord-014597-66vd2mdu	2406	10	in	in	IN
cord-014597-66vd2mdu	2406	11	Human	Human	NNP
cord-014597-66vd2mdu	2406	12	Embryonic	Embryonic	NNP
cord-014597-66vd2mdu	2406	13	Stem	stem	NN
cord-014597-66vd2mdu	2406	14	Cells	cell	NNS
cord-014597-66vd2mdu	2407	1	Systematic	systematic	JJ
cord-014597-66vd2mdu	2407	2	comparison	comparison	NN
cord-014597-66vd2mdu	2407	3	of	of	IN
cord-014597-66vd2mdu	2407	4	constitutive	constitutive	JJ
cord-014597-66vd2mdu	2407	5	promoters	promoter	NNS
cord-014597-66vd2mdu	2407	6	and	and	CC
cord-014597-66vd2mdu	2407	7	the	the	DT
cord-014597-66vd2mdu	2407	8	doxycycline	doxycycline	NN
cord-014597-66vd2mdu	2407	9	-	-	HYPH
cord-014597-66vd2mdu	2407	10	inducible	inducible	JJ
cord-014597-66vd2mdu	2407	11	promoter	promoter	NN
cord-014597-66vd2mdu	2407	12	Baculovirus	Baculovirus	NNP
cord-014597-66vd2mdu	2407	13	-	-	HYPH
cord-014597-66vd2mdu	2407	14	induced	induce	VBN
cord-014597-66vd2mdu	2407	15	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2407	16	protein	protein	NN
cord-014597-66vd2mdu	2407	17	expression	expression	NN
cord-014597-66vd2mdu	2407	18	in	in	IN
cord-014597-66vd2mdu	2407	19	human	human	JJ
cord-014597-66vd2mdu	2407	20	mesenchymal	mesenchymal	JJ
cord-014597-66vd2mdu	2407	21	stromal	stromal	JJ
cord-014597-66vd2mdu	2407	22	stem	stem	NN
cord-014597-66vd2mdu	2407	23	cells	cell	NNS
cord-014597-66vd2mdu	2407	24	:	:	:
cord-014597-66vd2mdu	2407	25	A	a	DT
cord-014597-66vd2mdu	2407	26	promoter	promoter	NN
cord-014597-66vd2mdu	2407	27	study	study	NN
cord-014597-66vd2mdu	2407	28	Triple	Triple	NNP
cord-014597-66vd2mdu	2407	29	-	-	HYPH
cord-014597-66vd2mdu	2407	30	Negative	negative	JJ
cord-014597-66vd2mdu	2407	31	Breast	Breast	NNP
cord-014597-66vd2mdu	2407	32	Cancer	Cancer	NNP
cord-014597-66vd2mdu	2407	33	:	:	:
cord-014597-66vd2mdu	2408	1	An	an	DT
cord-014597-66vd2mdu	2408	2	Unmet	unmet	JJ
cord-014597-66vd2mdu	2408	3	Medical	Medical	NNP
cord-014597-66vd2mdu	2408	4	Need	need	NN
cord-014597-66vd2mdu	2408	5	The	the	DT
cord-014597-66vd2mdu	2408	6	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2408	7	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	2408	8	antibody	antibody	NN
cord-014597-66vd2mdu	2408	9	market	market	NN
cord-014597-66vd2mdu	2408	10	.	.	.
cord-014597-66vd2mdu	2409	1	mabs	mabs	NNP
cord-014597-66vd2mdu	2409	2	A	a	DT
cord-014597-66vd2mdu	2409	3	monoclonal	monoclonal	JJ
cord-014597-66vd2mdu	2409	4	Antibody	Antibody	NNP
cord-014597-66vd2mdu	2409	5	against	against	IN
cord-014597-66vd2mdu	2409	6	Human	Human	NNP
cord-014597-66vd2mdu	2409	7	Notch1	Notch1	NNP
cord-014597-66vd2mdu	2409	8	Ligand	Ligand	NNP
cord-014597-66vd2mdu	2409	9	-	-	HYPH
cord-014597-66vd2mdu	2409	10	Binding	Binding	NNP
cord-014597-66vd2mdu	2409	11	Domain	Domain	NNP
cord-014597-66vd2mdu	2409	12	Depletes	deplete	VBZ
cord-014597-66vd2mdu	2409	13	Subpopulation	Subpopulation	NNP
cord-014597-66vd2mdu	2409	14	of	of	IN
cord-014597-66vd2mdu	2409	15	Putative	Putative	NNP
cord-014597-66vd2mdu	2409	16	Breast	Breast	NNP
cord-014597-66vd2mdu	2409	17	Cancer	Cancer	NNP
cord-014597-66vd2mdu	2410	1	Stem	stem	NN
cord-014597-66vd2mdu	2410	2	-	-	HYPH
cord-014597-66vd2mdu	2410	3	like	like	JJ
cord-014597-66vd2mdu	2410	4	Cells	cell	NNS
cord-014597-66vd2mdu	2410	5	Notch	Notch	NNP
cord-014597-66vd2mdu	2410	6	activation	activation	NN
cord-014597-66vd2mdu	2410	7	stimulates	stimulate	VBZ
cord-014597-66vd2mdu	2410	8	migration	migration	NN
cord-014597-66vd2mdu	2410	9	of	of	IN
cord-014597-66vd2mdu	2410	10	breast	breast	NN
cord-014597-66vd2mdu	2410	11	cancer	cancer	NN
cord-014597-66vd2mdu	2410	12	cells	cell	NNS
cord-014597-66vd2mdu	2410	13	and	and	CC
cord-014597-66vd2mdu	2410	14	promotes	promote	VBZ
cord-014597-66vd2mdu	2410	15	tumor	tumor	NN
cord-014597-66vd2mdu	2410	16	growth	growth	NN
cord-014597-66vd2mdu	2410	17	Notch	notch	NN
cord-014597-66vd2mdu	2410	18	-	-	HYPH
cord-014597-66vd2mdu	2410	19	out	out	NN
cord-014597-66vd2mdu	2410	20	for	for	IN
cord-014597-66vd2mdu	2410	21	breast	breast	NN
cord-014597-66vd2mdu	2410	22	cancer	cancer	NN
cord-014597-66vd2mdu	2410	23	therapies	therapy	NNS
cord-014597-66vd2mdu	2411	1	AAV	AAV	NNP
cord-014597-66vd2mdu	2411	2	production	production	NN
cord-014597-66vd2mdu	2411	3	in	in	IN
cord-014597-66vd2mdu	2411	4	suspension	suspension	NN
cord-014597-66vd2mdu	2411	5	:	:	:
cord-014597-66vd2mdu	2411	6	evaluation	evaluation	NN
cord-014597-66vd2mdu	2411	7	of	of	IN
cord-014597-66vd2mdu	2411	8	different	different	JJ
cord-014597-66vd2mdu	2411	9	cell	cell	NN
cord-014597-66vd2mdu	2411	10	culture	culture	NN
cord-014597-66vd2mdu	2411	11	media	medium	NNS
cord-014597-66vd2mdu	2411	12	and	and	CC
cord-014597-66vd2mdu	2411	13	scale	scale	NN
cord-014597-66vd2mdu	2411	14	-	-	HYPH
cord-014597-66vd2mdu	2411	15	up	up	NN
cord-014597-66vd2mdu	2411	16	potential	potential	JJ
cord-014597-66vd2mdu	2411	17	Modular	modular	JJ
cord-014597-66vd2mdu	2411	18	adeno	adeno	NN
cord-014597-66vd2mdu	2411	19	-	-	HYPH
cord-014597-66vd2mdu	2411	20	associated	associate	VBN
cord-014597-66vd2mdu	2411	21	virus	virus	NN
cord-014597-66vd2mdu	2411	22	(	(	-LRB-
cord-014597-66vd2mdu	2411	23	rAAV	rAAV	NNP
cord-014597-66vd2mdu	2411	24	)	)	-RRB-
cord-014597-66vd2mdu	2411	25	vectors	vector	NNS
cord-014597-66vd2mdu	2411	26	used	use	VBN
cord-014597-66vd2mdu	2411	27	for	for	IN
cord-014597-66vd2mdu	2411	28	cellular	cellular	JJ
cord-014597-66vd2mdu	2411	29	virusdirected	virusdirected	JJ
cord-014597-66vd2mdu	2411	30	enzyme	enzyme	NN
cord-014597-66vd2mdu	2411	31	prodrug	prodrug	NN
cord-014597-66vd2mdu	2411	32	therapy	therapy	NN
cord-014597-66vd2mdu	2411	33	Production	production	NN
cord-014597-66vd2mdu	2411	34	of	of	IN
cord-014597-66vd2mdu	2411	35	Recombinant	Recombinant	NNP
cord-014597-66vd2mdu	2411	36	Adenoassociated	Adenoassociated	NNP
cord-014597-66vd2mdu	2411	37	Virus	Virus	NNP
cord-014597-66vd2mdu	2411	38	Vectors	vector	NNS
cord-014597-66vd2mdu	2411	39	Using	use	VBG
cord-014597-66vd2mdu	2411	40	Suspension	Suspension	NNP
cord-014597-66vd2mdu	2411	41	HEK293	HEK293	NNP
cord-014597-66vd2mdu	2411	42	Cells	Cells	NNPS
cord-014597-66vd2mdu	2411	43	and	and	CC
cord-014597-66vd2mdu	2411	44	Continuous	continuous	JJ
cord-014597-66vd2mdu	2411	45	Harvest	Harvest	NNP
cord-014597-66vd2mdu	2411	46	of	of	IN
cord-014597-66vd2mdu	2411	47	Vector	Vector	NNP
cord-014597-66vd2mdu	2411	48	From	from	IN
cord-014597-66vd2mdu	2411	49	the	the	DT
cord-014597-66vd2mdu	2411	50	Culture	Culture	NNP
cord-014597-66vd2mdu	2411	51	Media	medium	NNS
cord-014597-66vd2mdu	2411	52	for	for	IN
cord-014597-66vd2mdu	2411	53	GMP	GMP	NNP
cord-014597-66vd2mdu	2411	54	FIX	FIX	NNP
cord-014597-66vd2mdu	2411	55	and	and	CC
cord-014597-66vd2mdu	2411	56	FLT1	FLT1	NNP
cord-014597-66vd2mdu	2411	57	Clinical	Clinical	NNP
cord-014597-66vd2mdu	2411	58	Vector	Vector	NNP
cord-014597-66vd2mdu	2411	59	Development	Development	NNP
cord-014597-66vd2mdu	2411	60	of	of	IN
cord-014597-66vd2mdu	2411	61	a	a	DT
cord-014597-66vd2mdu	2411	62	cost	cost	NN
cord-014597-66vd2mdu	2411	63	-	-	HYPH
cord-014597-66vd2mdu	2411	64	efficient	efficient	JJ
cord-014597-66vd2mdu	2411	65	scalable	scalable	JJ
cord-014597-66vd2mdu	2411	66	production	production	NN
cord-014597-66vd2mdu	2411	67	process	process	NN
cord-014597-66vd2mdu	2411	68	for	for	IN
cord-014597-66vd2mdu	2411	69	RAAV-8	RAAV-8	NNP
cord-014597-66vd2mdu	2411	70	based	base	VBN
cord-014597-66vd2mdu	2411	71	gene	gene	NN
cord-014597-66vd2mdu	2411	72	therapy	therapy	NN
cord-014597-66vd2mdu	2411	73	by	by	IN
cord-014597-66vd2mdu	2411	74	transfection	transfection	NN
cord-014597-66vd2mdu	2411	75	of	of	IN
cord-014597-66vd2mdu	2411	76	Hek-293	Hek-293	NNP
cord-014597-66vd2mdu	2411	77	cells	cell	NNS
cord-014597-66vd2mdu	2411	78	Simon	Simon	NNP
cord-014597-66vd2mdu	2411	79	Arias	Arias	NNP
cord-014597-66vd2mdu	2411	80	2	2	CD
cord-014597-66vd2mdu	2411	81	,	,	,
cord-014597-66vd2mdu	2411	82	Mustapha	Mustapha	NNP
cord-014597-66vd2mdu	2411	83	Hohoud	Hohoud	NNP
cord-014597-66vd2mdu	2411	84	1	1	CD
cord-014597-66vd2mdu	2411	85	,	,	,
cord-014597-66vd2mdu	2411	86	Roel	Roel	NNP
cord-014597-66vd2mdu	2411	87	Lievrouw	Lievrouw	NNP
cord-014597-66vd2mdu	2411	88	1	1	CD
cord-014597-66vd2mdu	2411	89	,	,	,
cord-014597-66vd2mdu	2411	90	Fabien	Fabien	NNP
cord-014597-66vd2mdu	2411	91	Moncaubeig	Moncaubeig	NNP
cord-014597-66vd2mdu	2411	92	1	1	CD
cord-014597-66vd2mdu	2412	1	B-1120	B-1120	NNP
cord-014597-66vd2mdu	2412	2	Brussels	Brussels	NNP
cord-014597-66vd2mdu	2412	3	,	,	,
cord-014597-66vd2mdu	2412	4	Belgium	Belgium	NNP
cord-014597-66vd2mdu	2412	5	;	;	:
cord-014597-66vd2mdu	2412	6	2	2	CD
cord-014597-66vd2mdu	2412	7	Généthon	Généthon	NNP
cord-014597-66vd2mdu	2412	8	,	,	,
cord-014597-66vd2mdu	2412	9	Rue	Rue	NNP
cord-014597-66vd2mdu	2412	10	de	de	NNP
cord-014597-66vd2mdu	2412	11	l'Internationale	l'Internationale	NNP
cord-014597-66vd2mdu	2412	12	1	1	CD
cord-014597-66vd2mdu	2412	13	Cell	Cell	NNP
cord-014597-66vd2mdu	2412	14	-	-	HYPH
cord-014597-66vd2mdu	2412	15	Free	Free	NNP
cord-014597-66vd2mdu	2412	16	Systems	Systems	NNPS
cord-014597-66vd2mdu	2412	17	Based	base	VBN
cord-014597-66vd2mdu	2412	18	on	on	IN
cord-014597-66vd2mdu	2412	19	CHO	CHO	NNP
cord-014597-66vd2mdu	2412	20	Cell	Cell	NNP
cord-014597-66vd2mdu	2412	21	Lysates	Lysates	NNP
cord-014597-66vd2mdu	2412	22	:	:	:
cord-014597-66vd2mdu	2413	1	Optimization	Optimization	NNP
cord-014597-66vd2mdu	2413	2	Strategies	Strategies	NNPS
cord-014597-66vd2mdu	2413	3	,	,	,
cord-014597-66vd2mdu	2413	4	Synthesis	Synthesis	NNP
cord-014597-66vd2mdu	2413	5	of	of	IN
cord-014597-66vd2mdu	2413	6	"	"	``
cord-014597-66vd2mdu	2413	7	Difficult	difficult	JJ
cord-014597-66vd2mdu	2413	8	-	-	HYPH
cord-014597-66vd2mdu	2413	9	to	to	IN
cord-014597-66vd2mdu	2413	10	-	-	HYPH
cord-014597-66vd2mdu	2413	11	Express	Express	NNP
cord-014597-66vd2mdu	2413	12	"	"	``
cord-014597-66vd2mdu	2413	13	Proteins	Proteins	NNPS
cord-014597-66vd2mdu	2413	14	and	and	CC
cord-014597-66vd2mdu	2413	15	Future	Future	NNP
cord-014597-66vd2mdu	2413	16	Perspectives	Perspectives	NNPS
cord-014597-66vd2mdu	2413	17	Cell	cell	NN
cord-014597-66vd2mdu	2413	18	-	-	HYPH
cord-014597-66vd2mdu	2413	19	free	free	JJ
cord-014597-66vd2mdu	2413	20	protein	protein	NN
cord-014597-66vd2mdu	2413	21	expression	expression	NN
cord-014597-66vd2mdu	2413	22	based	base	VBN
cord-014597-66vd2mdu	2413	23	on	on	IN
cord-014597-66vd2mdu	2413	24	extracts	extract	NNS
cord-014597-66vd2mdu	2413	25	from	from	IN
cord-014597-66vd2mdu	2413	26	CHO	CHO	NNP
cord-014597-66vd2mdu	2413	27	cells	cell	NNS
cord-014597-66vd2mdu	2413	28	Comparison	comparison	NN
cord-014597-66vd2mdu	2413	29	of	of	IN
cord-014597-66vd2mdu	2413	30	cell	cell	NN
cord-014597-66vd2mdu	2413	31	-	-	HYPH
cord-014597-66vd2mdu	2413	32	based	base	VBN
cord-014597-66vd2mdu	2413	33	vs.	vs.	IN
cord-014597-66vd2mdu	2413	34	cell	cell	NN
cord-014597-66vd2mdu	2413	35	-	-	HYPH
cord-014597-66vd2mdu	2413	36	free	free	JJ
cord-014597-66vd2mdu	2413	37	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2413	38	systems	system	NNS
cord-014597-66vd2mdu	2413	39	for	for	IN
cord-014597-66vd2mdu	2413	40	the	the	DT
cord-014597-66vd2mdu	2413	41	production	production	NN
cord-014597-66vd2mdu	2413	42	of	of	IN
cord-014597-66vd2mdu	2413	43	a	a	DT
cord-014597-66vd2mdu	2413	44	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2413	45	human	human	JJ
cord-014597-66vd2mdu	2413	46	bone	bone	NN
cord-014597-66vd2mdu	2413	47	morphogenic	morphogenic	JJ
cord-014597-66vd2mdu	2413	48	growth	growth	NN
cord-014597-66vd2mdu	2413	49	factor	factor	NN
cord-014597-66vd2mdu	2413	50	IRES	IRES	NNP
cord-014597-66vd2mdu	2413	51	-	-	HYPH
cord-014597-66vd2mdu	2413	52	Mediated	mediate	VBN
cord-014597-66vd2mdu	2413	53	Translation	translation	NN
cord-014597-66vd2mdu	2413	54	of	of	IN
cord-014597-66vd2mdu	2413	55	Membrane	Membrane	NNP
cord-014597-66vd2mdu	2413	56	Proteins	Proteins	NNPS
cord-014597-66vd2mdu	2413	57	and	and	CC
cord-014597-66vd2mdu	2413	58	Glycoproteins	Glycoproteins	NNPS
cord-014597-66vd2mdu	2413	59	in	in	IN
cord-014597-66vd2mdu	2413	60	Production	production	NN
cord-014597-66vd2mdu	2413	61	of	of	IN
cord-014597-66vd2mdu	2413	62	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2413	63	factor	factor	NN
cord-014597-66vd2mdu	2413	64	VII	vii	NN
cord-014597-66vd2mdu	2413	65	in	in	IN
cord-014597-66vd2mdu	2413	66	Sk	Sk	NNP
cord-014597-66vd2mdu	2413	67	-	-	HYPH
cord-014597-66vd2mdu	2413	68	Hep-1	Hep-1	NNP
cord-014597-66vd2mdu	2413	69	human	human	JJ
cord-014597-66vd2mdu	2413	70	cell	cell	NN
cord-014597-66vd2mdu	2413	71	line	line	NN
cord-014597-66vd2mdu	2413	72	ZIP	zip	NN
cord-014597-66vd2mdu	2413	73	14049	14049	CD
cord-014597-66vd2mdu	2413	74	-	-	SYM
cord-014597-66vd2mdu	2413	75	900	900	CD
cord-014597-66vd2mdu	2413	76	,	,	,
cord-014597-66vd2mdu	2413	77	Brazil	Brazil	NNP
cord-014597-66vd2mdu	2413	78	;	;	:
cord-014597-66vd2mdu	2413	79	3	3	CD
cord-014597-66vd2mdu	2413	80	Department	Department	NNP
cord-014597-66vd2mdu	2413	81	of	of	IN
cord-014597-66vd2mdu	2413	82	Clinical	Clinical	NNP
cord-014597-66vd2mdu	2413	83	,	,	,
cord-014597-66vd2mdu	2413	84	Toxicological	toxicological	JJ
cord-014597-66vd2mdu	2413	85	and	and	CC
cord-014597-66vd2mdu	2413	86	Food	Food	NNP
cord-014597-66vd2mdu	2413	87	Science	Science	NNP
cord-014597-66vd2mdu	2413	88	Analysis	Analysis	NNP
cord-014597-66vd2mdu	2413	89	,	,	,
cord-014597-66vd2mdu	2413	90	Faculty	Faculty	NNP
cord-014597-66vd2mdu	2413	91	of	of	IN
cord-014597-66vd2mdu	2413	92	Pharmaceutical	Pharmaceutical	NNP
cord-014597-66vd2mdu	2413	93	Sciences	Sciences	NNPS
cord-014597-66vd2mdu	2413	94	of	of	IN
cord-014597-66vd2mdu	2413	95	Ribeirão	Ribeirão	NNP
cord-014597-66vd2mdu	2413	96	Preto	Preto	NNP
cord-014597-66vd2mdu	2413	97	Human	Human	NNP
cord-014597-66vd2mdu	2413	98	cell	cell	NN
cord-014597-66vd2mdu	2413	99	lines	line	NNS
cord-014597-66vd2mdu	2413	100	for	for	IN
cord-014597-66vd2mdu	2413	101	the	the	DT
cord-014597-66vd2mdu	2413	102	production	production	NN
cord-014597-66vd2mdu	2413	103	of	of	IN
cord-014597-66vd2mdu	2413	104	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2413	105	proteins	protein	NNS
cord-014597-66vd2mdu	2413	106	:	:	:
cord-014597-66vd2mdu	2413	107	on	on	IN
cord-014597-66vd2mdu	2413	108	the	the	DT
cord-014597-66vd2mdu	2413	109	horizon	horizon	NN
cord-014597-66vd2mdu	2413	110	Production	Production	NNP
cord-014597-66vd2mdu	2413	111	of	of	IN
cord-014597-66vd2mdu	2413	112	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2413	113	protein	protein	NN
cord-014597-66vd2mdu	2413	114	therapeutics	therapeutic	NNS
cord-014597-66vd2mdu	2413	115	in	in	IN
cord-014597-66vd2mdu	2413	116	cultivated	cultivate	VBN
cord-014597-66vd2mdu	2413	117	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2413	118	cells	cell	NNS
cord-014597-66vd2mdu	2414	1	Recombinant	recombinant	JJ
cord-014597-66vd2mdu	2414	2	protein	protein	NN
cord-014597-66vd2mdu	2414	3	therapeutics	therapeutic	NNS
cord-014597-66vd2mdu	2414	4	from	from	IN
cord-014597-66vd2mdu	2414	5	cho	cho	NNP
cord-014597-66vd2mdu	2414	6	cells	cell	NNS
cord-014597-66vd2mdu	2414	7	-20	-20	CD
cord-014597-66vd2mdu	2414	8	years	year	NNS
cord-014597-66vd2mdu	2414	9	and	and	CC
cord-014597-66vd2mdu	2414	10	counting	count	VBG
cord-014597-66vd2mdu	2414	11	Establishment	Establishment	NNP
cord-014597-66vd2mdu	2414	12	of	of	IN
cord-014597-66vd2mdu	2414	13	a	a	DT
cord-014597-66vd2mdu	2414	14	cell	cell	NN
cord-014597-66vd2mdu	2414	15	line	line	NN
cord-014597-66vd2mdu	2414	16	expressing	express	VBG
cord-014597-66vd2mdu	2414	17	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2414	18	factor	factor	NN
cord-014597-66vd2mdu	2414	19	VII	vii	NN
cord-014597-66vd2mdu	2414	20	and	and	CC
cord-014597-66vd2mdu	2414	21	its	-PRON-	PRP$
cord-014597-66vd2mdu	2414	22	subsequent	subsequent	JJ
cord-014597-66vd2mdu	2414	23	conversion	conversion	NN
cord-014597-66vd2mdu	2414	24	to	to	IN
cord-014597-66vd2mdu	2414	25	active	active	JJ
cord-014597-66vd2mdu	2414	26	form	form	NN
cord-014597-66vd2mdu	2414	27	FVIIa	FVIIa	NNS
cord-014597-66vd2mdu	2414	28	through	through	IN
cord-014597-66vd2mdu	2414	29	hepsin	hepsin	NNP
cord-014597-66vd2mdu	2414	30	by	by	IN
cord-014597-66vd2mdu	2414	31	genetic	genetic	JJ
cord-014597-66vd2mdu	2414	32	engineering	engineering	NN
cord-014597-66vd2mdu	2414	33	method	method	NN
cord-014597-66vd2mdu	2414	34	Expression	expression	NN
cord-014597-66vd2mdu	2414	35	and	and	CC
cord-014597-66vd2mdu	2414	36	fast	fast	JJ
cord-014597-66vd2mdu	2414	37	preparation	preparation	NN
cord-014597-66vd2mdu	2414	38	of	of	IN
cord-014597-66vd2mdu	2414	39	biologically	biologically	RB
cord-014597-66vd2mdu	2414	40	active	active	JJ
cord-014597-66vd2mdu	2414	41	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2414	42	human	human	JJ
cord-014597-66vd2mdu	2414	43	coagulation	coagulation	NN
cord-014597-66vd2mdu	2414	44	factor	factor	NN
cord-014597-66vd2mdu	2414	45	VII	vii	NN
cord-014597-66vd2mdu	2414	46	in	in	IN
cord-014597-66vd2mdu	2414	47	CHO	CHO	NNP
cord-014597-66vd2mdu	2414	48	-	-	HYPH
cord-014597-66vd2mdu	2414	49	K1	K1	NNP
cord-014597-66vd2mdu	2414	50	cells	cell	NNS
cord-014597-66vd2mdu	2414	51	Implications	implication	NNS
cord-014597-66vd2mdu	2414	52	of	of	IN
cord-014597-66vd2mdu	2414	53	the	the	DT
cord-014597-66vd2mdu	2414	54	presence	presence	NN
cord-014597-66vd2mdu	2414	55	of	of	IN
cord-014597-66vd2mdu	2414	56	N	N	NNP
cord-014597-66vd2mdu	2414	57	-	-	HYPH
cord-014597-66vd2mdu	2414	58	glycolylneuraminic	glycolylneuraminic	NN
cord-014597-66vd2mdu	2414	59	acid	acid	NN
cord-014597-66vd2mdu	2414	60	in	in	IN
cord-014597-66vd2mdu	2414	61	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2414	62	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2414	63	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	2415	1	Uniquely	uniquely	RB
cord-014597-66vd2mdu	2415	2	human	human	JJ
cord-014597-66vd2mdu	2415	3	evolution	evolution	NN
cord-014597-66vd2mdu	2415	4	of	of	IN
cord-014597-66vd2mdu	2415	5	sialic	sialic	JJ
cord-014597-66vd2mdu	2415	6	acid	acid	NN
cord-014597-66vd2mdu	2415	7	genetics	genetic	NNS
cord-014597-66vd2mdu	2415	8	and	and	CC
cord-014597-66vd2mdu	2415	9	biology	biology	NN
cord-014597-66vd2mdu	2415	10	Production	production	NN
cord-014597-66vd2mdu	2415	11	platforms	platform	NNS
cord-014597-66vd2mdu	2415	12	for	for	IN
cord-014597-66vd2mdu	2415	13	biotherapeutic	biotherapeutic	JJ
cord-014597-66vd2mdu	2415	14	glycoproteins	glycoprotein	NNS
cord-014597-66vd2mdu	2415	15	.	.	.
cord-014597-66vd2mdu	2416	1	Occurrence	occurrence	NN
cord-014597-66vd2mdu	2416	2	,	,	,
cord-014597-66vd2mdu	2416	3	impact	impact	NN
cord-014597-66vd2mdu	2416	4	,	,	,
cord-014597-66vd2mdu	2416	5	and	and	CC
cord-014597-66vd2mdu	2416	6	challenges	challenge	NNS
cord-014597-66vd2mdu	2416	7	of	of	IN
cord-014597-66vd2mdu	2416	8	non	non	JJ
cord-014597-66vd2mdu	2416	9	-	-	JJ
cord-014597-66vd2mdu	2416	10	human	human	JJ
cord-014597-66vd2mdu	2416	11	sialylation	sialylation	NN
cord-014597-66vd2mdu	2416	12	Human	Human	NNP
cord-014597-66vd2mdu	2416	13	cells	cell	NNS
cord-014597-66vd2mdu	2416	14	:	:	:
cord-014597-66vd2mdu	2416	15	new	new	JJ
cord-014597-66vd2mdu	2416	16	platform	platform	NN
cord-014597-66vd2mdu	2416	17	for	for	IN
cord-014597-66vd2mdu	2416	18	recombinant	recombinant	JJ
cord-014597-66vd2mdu	2416	19	therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2416	20	protein	protein	NN
cord-014597-66vd2mdu	2416	21	production	production	NN
cord-014597-66vd2mdu	2416	22	Therapeutic	therapeutic	JJ
cord-014597-66vd2mdu	2416	23	glycoprotein	glycoprotein	NN
cord-014597-66vd2mdu	2416	24	production	production	NN
cord-014597-66vd2mdu	2416	25	in	in	IN
cord-014597-66vd2mdu	2416	26	mammalian	mammalian	JJ
cord-014597-66vd2mdu	2416	27	cells	cell	NNS
cord-014597-66vd2mdu	2417	1	Masthering	masthere	VBG
cord-014597-66vd2mdu	2417	2	industrialization	industrialization	NN
cord-014597-66vd2mdu	2417	3	of	of	IN
cord-014597-66vd2mdu	2417	4	cell	cell	NN
cord-014597-66vd2mdu	2417	5	therapy	therapy	NN
cord-014597-66vd2mdu	2417	6	products	product	NNS
cord-014597-66vd2mdu	2418	1	Tissue	tissue	NN
cord-014597-66vd2mdu	2418	2	cells	cell	NNS
cord-014597-66vd2mdu	2418	3	feel	feel	VBP
cord-014597-66vd2mdu	2418	4	and	and	CC
cord-014597-66vd2mdu	2418	5	respond	respond	VBP
cord-014597-66vd2mdu	2418	6	to	to	IN
cord-014597-66vd2mdu	2418	7	the	the	DT
cord-014597-66vd2mdu	2418	8	stiffness	stiffness	NN
cord-014597-66vd2mdu	2418	9	of	of	IN
cord-014597-66vd2mdu	2418	10	their	-PRON-	PRP$
cord-014597-66vd2mdu	2418	11	substrate	substrate	NN
cord-014597-66vd2mdu	2418	12	Matrix	Matrix	NNP
cord-014597-66vd2mdu	2418	13	elasticity	elasticity	NN
cord-014597-66vd2mdu	2418	14	directs	direct	VBZ
cord-014597-66vd2mdu	2418	15	stem	stem	NN
cord-014597-66vd2mdu	2418	16	cell	cell	NN
cord-014597-66vd2mdu	2418	17	Lineage	lineage	NN
cord-014597-66vd2mdu	2418	18	specification	specification	NN
cord-014597-66vd2mdu	2418	19	Continuous	continuous	JJ
cord-014597-66vd2mdu	2418	20	cider	cider	NN
cord-014597-66vd2mdu	2418	21	fermentation	fermentation	NN
cord-014597-66vd2mdu	2418	22	with	with	IN
cord-014597-66vd2mdu	2418	23	co	co	JJ
cord-014597-66vd2mdu	2418	24	-	-	JJ
cord-014597-66vd2mdu	2418	25	immobilized	immobilize	VBN
cord-014597-66vd2mdu	2418	26	yeast	yeast	NN
cord-014597-66vd2mdu	2418	27	and	and	CC
cord-014597-66vd2mdu	2418	28	Leuconostoc	Leuconostoc	NNP
cord-014597-66vd2mdu	2418	29	oenos	oenos	NN
cord-014597-66vd2mdu	2418	30	cells	cell	VBZ
cord-014597-66vd2mdu	2418	31	Eternity	eternity	NN
cord-014597-66vd2mdu	2418	32	and	and	CC
cord-014597-66vd2mdu	2418	33	functionality	functionality	NN
cord-014597-66vd2mdu	2418	34	-rational	-rational	JJ
cord-014597-66vd2mdu	2418	35	access	access	NN
cord-014597-66vd2mdu	2418	36	to	to	IN
cord-014597-66vd2mdu	2418	37	physiologically	physiologically	RB
cord-014597-66vd2mdu	2418	38	relevant	relevant	JJ
cord-014597-66vd2mdu	2418	39	cell	cell	NN
cord-014597-66vd2mdu	2418	40	lines	line	NNS
cord-014597-66vd2mdu	2418	41	Generation	generation	NN
cord-014597-66vd2mdu	2418	42	and	and	CC
cord-014597-66vd2mdu	2418	43	characterization	characterization	NN
cord-014597-66vd2mdu	2418	44	of	of	IN
cord-014597-66vd2mdu	2418	45	two	two	CD
cord-014597-66vd2mdu	2418	46	immortalized	immortalize	VBN
cord-014597-66vd2mdu	2418	47	human	human	JJ
cord-014597-66vd2mdu	2418	48	osteoblastic	osteoblastic	JJ
cord-014597-66vd2mdu	2418	49	cell	cell	NN
cord-014597-66vd2mdu	2418	50	lines	line	NNS
cord-014597-66vd2mdu	2418	51	useful	useful	JJ
cord-014597-66vd2mdu	2418	52	for	for	IN
cord-014597-66vd2mdu	2418	53	epigenetic	epigenetic	JJ
cord-014597-66vd2mdu	2418	54	studies	study	NNS
cord-014597-66vd2mdu	2418	55	Biocompatibility	Biocompatibility	NNP
cord-014597-66vd2mdu	2418	56	of	of	IN
cord-014597-66vd2mdu	2418	57	NanoShuttleTM	NanoShuttleTM	NNP
cord-014597-66vd2mdu	2418	58	and	and	CC
cord-014597-66vd2mdu	2419	1	the	the	DT
cord-014597-66vd2mdu	2419	2	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2419	3	field	field	NN
cord-014597-66vd2mdu	2419	4	in	in	IN
cord-014597-66vd2mdu	2419	5	magnetic	magnetic	JJ
cord-014597-66vd2mdu	2419	6	3D	3D	NNP
cord-014597-66vd2mdu	2419	7	bioprinting	bioprinting	NNP
cord-014597-66vd2mdu	2419	8	Publisher	Publisher	NNP
cord-014597-66vd2mdu	2419	9	's	's	POS
cord-014597-66vd2mdu	2419	10	Note	Note	NNP
cord-014597-66vd2mdu	2419	11	Springer	Springer	NNP
cord-014597-66vd2mdu	2419	12	Nature	Nature	NNP
cord-014597-66vd2mdu	2419	13	remains	remain	VBZ
cord-014597-66vd2mdu	2419	14	neutral	neutral	JJ
cord-014597-66vd2mdu	2419	15	with	with	IN
cord-014597-66vd2mdu	2419	16	regard	regard	NN
cord-014597-66vd2mdu	2419	17	to	to	IN
cord-014597-66vd2mdu	2419	18	jurisdictional	jurisdictional	JJ
cord-014597-66vd2mdu	2419	19	claims	claim	NNS
cord-014597-66vd2mdu	2419	20	in	in	IN
cord-014597-66vd2mdu	2419	21	published	publish	VBN
cord-014597-66vd2mdu	2419	22	maps	map	NNS
cord-014597-66vd2mdu	2419	23	and	and	CC
cord-014597-66vd2mdu	2419	24	institutional	institutional	JJ
cord-014597-66vd2mdu	2419	25	affiliations	affiliation	NNS
cord-014597-66vd2mdu	2420	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2420	2	accept	accept	VBP
cord-014597-66vd2mdu	2420	3	pre	pre	JJ
cord-014597-66vd2mdu	2420	4	-	-	JJ
cord-014597-66vd2mdu	2420	5	submission	submission	JJ
cord-014597-66vd2mdu	2420	6	inquiries	inquiry	NNS
cord-014597-66vd2mdu	2421	1	•	•	NNP
cord-014597-66vd2mdu	2422	1	Our	-PRON-	PRP$
cord-014597-66vd2mdu	2422	2	selector	selector	NN
cord-014597-66vd2mdu	2422	3	tool	tool	NN
cord-014597-66vd2mdu	2422	4	helps	help	VBZ
cord-014597-66vd2mdu	2422	5	you	-PRON-	PRP
cord-014597-66vd2mdu	2422	6	to	to	TO
cord-014597-66vd2mdu	2422	7	find	find	VB
cord-014597-66vd2mdu	2422	8	the	the	DT
cord-014597-66vd2mdu	2422	9	most	most	RBS
cord-014597-66vd2mdu	2422	10	relevant	relevant	JJ
cord-014597-66vd2mdu	2422	11	journal	journal	NN
cord-014597-66vd2mdu	2423	1	•	•	NNP
cord-014597-66vd2mdu	2424	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2424	2	provide	provide	VBP
cord-014597-66vd2mdu	2424	3	round	round	IN
cord-014597-66vd2mdu	2424	4	the	the	DT
cord-014597-66vd2mdu	2424	5	clock	clock	NN
cord-014597-66vd2mdu	2424	6	customer	customer	NN
cord-014597-66vd2mdu	2424	7	support	support	NN
cord-014597-66vd2mdu	2425	1	•	•	NNP
cord-014597-66vd2mdu	2426	1	Convenient	convenient	JJ
cord-014597-66vd2mdu	2426	2	online	online	JJ
cord-014597-66vd2mdu	2426	3	submission	submission	NN
cord-014597-66vd2mdu	2427	1	•	•	NNP
cord-014597-66vd2mdu	2428	1	Thorough	thorough	JJ
cord-014597-66vd2mdu	2428	2	peer	peer	NN
cord-014597-66vd2mdu	2428	3	review	review	NN
cord-014597-66vd2mdu	2429	1	•	•	NNP
cord-014597-66vd2mdu	2430	1	Inclusion	inclusion	NN
cord-014597-66vd2mdu	2430	2	in	in	IN
cord-014597-66vd2mdu	2430	3	PubMed	PubMed	NNP
cord-014597-66vd2mdu	2430	4	and	and	CC
cord-014597-66vd2mdu	2430	5	all	all	DT
cord-014597-66vd2mdu	2430	6	major	major	JJ
cord-014597-66vd2mdu	2430	7	indexing	indexing	NN
cord-014597-66vd2mdu	2430	8	services	service	NNS
cord-014597-66vd2mdu	2431	1	•	•	NNP
cord-014597-66vd2mdu	2432	1	Maximum	maximum	JJ
cord-014597-66vd2mdu	2432	2	visibility	visibility	NN
cord-014597-66vd2mdu	2432	3	for	for	IN
cord-014597-66vd2mdu	2432	4	your	-PRON-	PRP$
cord-014597-66vd2mdu	2432	5	research	research	NN
cord-014597-66vd2mdu	2432	6	Submit	submit	VB
cord-014597-66vd2mdu	2432	7	your	-PRON-	PRP$
cord-014597-66vd2mdu	2432	8	manuscript	manuscript	NN
cord-014597-66vd2mdu	2432	9	at	at	IN
cord-014597-66vd2mdu	2432	10	www	www	NNP
cord-014597-66vd2mdu	2432	11	Submit	submit	VB
cord-014597-66vd2mdu	2432	12	your	-PRON-	PRP$
cord-014597-66vd2mdu	2432	13	next	next	JJ
cord-014597-66vd2mdu	2432	14	manuscript	manuscript	NN
cord-014597-66vd2mdu	2432	15	to	to	IN
cord-014597-66vd2mdu	2432	16	BioMed	BioMed	NNP
cord-014597-66vd2mdu	2432	17	Central	Central	NNP
cord-014597-66vd2mdu	2432	18	and	and	CC
cord-014597-66vd2mdu	2432	19	we	-PRON-	PRP
cord-014597-66vd2mdu	2432	20	will	will	MD
cord-014597-66vd2mdu	2432	21	help	help	VB
cord-014597-66vd2mdu	2432	22	you	-PRON-	PRP
cord-014597-66vd2mdu	2432	23	at	at	IN
cord-014597-66vd2mdu	2432	24	every	every	DT
cord-014597-66vd2mdu	2432	25	step	step	NN
cord-014597-66vd2mdu	2433	1	Authors	author	NNS
cord-014597-66vd2mdu	2433	2	thankfully	thankfully	RB
cord-014597-66vd2mdu	2433	3	acknowledge	acknowledge	VBP
cord-014597-66vd2mdu	2433	4	the	the	DT
cord-014597-66vd2mdu	2433	5	Biotechnology	Biotechnology	NNP
cord-014597-66vd2mdu	2433	6	and	and	CC
cord-014597-66vd2mdu	2433	7	Biological	Biological	NNP
cord-014597-66vd2mdu	2433	8	Sciences	Sciences	NNPS
cord-014597-66vd2mdu	2433	9	Research	Research	NNP
cord-014597-66vd2mdu	2433	10	Council	Council	NNP
cord-014597-66vd2mdu	2433	11	for	for	IN
cord-014597-66vd2mdu	2433	12	funding	fund	VBG
cord-014597-66vd2mdu	2433	13	this	this	DT
cord-014597-66vd2mdu	2433	14	research	research	NN
cord-014597-66vd2mdu	2433	15	work	work	NN
cord-014597-66vd2mdu	2433	16	.	.	.
cord-014597-66vd2mdu	2434	1	SNS	SNS	NNP
cord-014597-66vd2mdu	2434	2	thanks	thank	NNS
cord-014597-66vd2mdu	2434	3	ESACT	ESACT	NNP
cord-014597-66vd2mdu	2434	4	2017	2017	CD
cord-014597-66vd2mdu	2434	5	for	for	IN
cord-014597-66vd2mdu	2434	6	providing	provide	VBG
cord-014597-66vd2mdu	2434	7	her	-PRON-	PRP
cord-014597-66vd2mdu	2434	8	with	with	IN
cord-014597-66vd2mdu	2434	9	the	the	DT
cord-014597-66vd2mdu	2434	10	opportunity	opportunity	NN
cord-014597-66vd2mdu	2434	11	to	to	TO
cord-014597-66vd2mdu	2434	12	present	present	VB
cord-014597-66vd2mdu	2434	13	her	-PRON-	PRP$
cord-014597-66vd2mdu	2434	14	work	work	NN
cord-014597-66vd2mdu	2434	15	at	at	IN
cord-014597-66vd2mdu	2434	16	the	the	DT
cord-014597-66vd2mdu	2434	17	meeting	meeting	NN
cord-014597-66vd2mdu	2434	18	.	.	.
cord-014597-66vd2mdu	2435	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2435	2	would	would	MD
cord-014597-66vd2mdu	2435	3	like	like	VB
cord-014597-66vd2mdu	2435	4	to	to	TO
cord-014597-66vd2mdu	2435	5	thank	thank	VB
cord-014597-66vd2mdu	2435	6	Moritz	Moritz	NNP
cord-014597-66vd2mdu	2435	7	Frei	Frei	NNP
cord-014597-66vd2mdu	2435	8	for	for	IN
cord-014597-66vd2mdu	2435	9	his	-PRON-	PRP$
cord-014597-66vd2mdu	2435	10	support	support	NN
cord-014597-66vd2mdu	2435	11	for	for	IN
cord-014597-66vd2mdu	2435	12	the	the	DT
cord-014597-66vd2mdu	2435	13	generation	generation	NN
cord-014597-66vd2mdu	2435	14	of	of	IN
cord-014597-66vd2mdu	2435	15	the	the	DT
cord-014597-66vd2mdu	2435	16	NGS	NGS	NNP
cord-014597-66vd2mdu	2435	17	transcriptomics	transcriptomics	NNPS
cord-014597-66vd2mdu	2435	18	data	datum	NNS
cord-014597-66vd2mdu	2435	19	.	.	.
cord-014597-66vd2mdu	2435	20	 	 	_SP
cord-014597-66vd2mdu	2436	1	Many	many	JJ
cord-014597-66vd2mdu	2436	2	thanks	thank	NNS
cord-014597-66vd2mdu	2436	3	to	to	IN
cord-014597-66vd2mdu	2436	4	Valentine	Valentine	NNP
cord-014597-66vd2mdu	2436	5	Chevallier	Chevallier	NNP
cord-014597-66vd2mdu	2436	6	for	for	IN
cord-014597-66vd2mdu	2436	7	her	-PRON-	PRP$
cord-014597-66vd2mdu	2436	8	precious	precious	JJ
cord-014597-66vd2mdu	2436	9	advices	advice	NNS
cord-014597-66vd2mdu	2436	10	,	,	,
cord-014597-66vd2mdu	2436	11	to	to	IN
cord-014597-66vd2mdu	2436	12	Stefanos	Stefanos	NNP
cord-014597-66vd2mdu	2436	13	Grammatikos	Grammatikos	NNP
cord-014597-66vd2mdu	2436	14	for	for	IN
cord-014597-66vd2mdu	2436	15	his	-PRON-	PRP$
cord-014597-66vd2mdu	2436	16	support	support	NN
cord-014597-66vd2mdu	2436	17	and	and	CC
cord-014597-66vd2mdu	2436	18	to	to	IN
cord-014597-66vd2mdu	2436	19	the	the	DT
cord-014597-66vd2mdu	2436	20	whole	whole	JJ
cord-014597-66vd2mdu	2436	21	Upstream	Upstream	NNP
cord-014597-66vd2mdu	2436	22	Process	Process	NNP
cord-014597-66vd2mdu	2436	23	Sciences	Sciences	NNPS
cord-014597-66vd2mdu	2436	24	team	team	NN
cord-014597-66vd2mdu	2436	25	.	.	.
cord-014597-66vd2mdu	2437	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2437	2	thank	thank	VBP
cord-014597-66vd2mdu	2437	3	David	David	NNP
cord-014597-66vd2mdu	2437	4	Bruehlmann	Bruehlmann	NNP
cord-014597-66vd2mdu	2437	5	and	and	CC
cord-014597-66vd2mdu	2437	6	Thomas	Thomas	NNP
cord-014597-66vd2mdu	2437	7	Vuillemin	Vuillemin	NNP
cord-014597-66vd2mdu	2437	8	from	from	IN
cord-014597-66vd2mdu	2437	9	Merck	Merck	NNP
cord-014597-66vd2mdu	2437	10	(	(	-LRB-
cord-014597-66vd2mdu	2437	11	Vevey	Vevey	NNP
cord-014597-66vd2mdu	2437	12	,	,	,
cord-014597-66vd2mdu	2437	13	Switzerland	Switzerland	NNP
cord-014597-66vd2mdu	2437	14	)	)	-RRB-
cord-014597-66vd2mdu	2437	15	for	for	IN
cord-014597-66vd2mdu	2437	16	providing	provide	VBG
cord-014597-66vd2mdu	2437	17	the	the	DT
cord-014597-66vd2mdu	2437	18	IgG	IgG	NNP
cord-014597-66vd2mdu	2437	19	glycan	glycan	NN
cord-014597-66vd2mdu	2437	20	variants	variant	NNS
cord-014597-66vd2mdu	2437	21	and	and	CC
cord-014597-66vd2mdu	2437	22	the	the	DT
cord-014597-66vd2mdu	2437	23	2-AB	2-ab	CD
cord-014597-66vd2mdu	2437	24	-	-	HYPH
cord-014597-66vd2mdu	2437	25	UPLC	UPLC	NNP
cord-014597-66vd2mdu	2437	26	glycan	glycan	NN
cord-014597-66vd2mdu	2437	27	data	datum	NNS
cord-014597-66vd2mdu	2437	28	.	.	.
cord-014597-66vd2mdu	2438	1	Polyplus	polyplus	NN
cord-014597-66vd2mdu	2438	2	-	-	HYPH
cord-014597-66vd2mdu	2438	3	transfection	transfection	NN
cord-014597-66vd2mdu	2438	4	would	would	MD
cord-014597-66vd2mdu	2438	5	like	like	VB
cord-014597-66vd2mdu	2438	6	to	to	TO
cord-014597-66vd2mdu	2438	7	thank	thank	VB
cord-014597-66vd2mdu	2438	8	Généthon	Généthon	NNP
cord-014597-66vd2mdu	2438	9	for	for	IN
cord-014597-66vd2mdu	2438	10	their	-PRON-	PRP$
cord-014597-66vd2mdu	2438	11	kindly	kindly	RB
cord-014597-66vd2mdu	2438	12	provided	provide	VBN
cord-014597-66vd2mdu	2438	13	data	datum	NNS
cord-014597-66vd2mdu	2438	14	.	.	.
cord-014597-66vd2mdu	2439	1	Acknowledgements	acknowledgement	NNS
cord-014597-66vd2mdu	2439	2	Asmita	Asmita	NNP
cord-014597-66vd2mdu	2439	3	Mukerji	Mukerji	NNP
cord-014597-66vd2mdu	2439	4	,	,	,
cord-014597-66vd2mdu	2439	5	Reetesh	Reetesh	NNP
cord-014597-66vd2mdu	2439	6	PM	PM	NNP
cord-014597-66vd2mdu	2439	7	,	,	,
cord-014597-66vd2mdu	2439	8	Sasi	Sasi	NNP
cord-014597-66vd2mdu	2439	9	Kumar	Kumar	NNP
cord-014597-66vd2mdu	2439	10	K	K	NNP
cord-014597-66vd2mdu	2439	11	,	,	,
cord-014597-66vd2mdu	2439	12	Pilot	Pilot	NNP
cord-014597-66vd2mdu	2439	13	plant	plant	NN
cord-014597-66vd2mdu	2439	14	team	team	NN
cord-014597-66vd2mdu	2439	15	 	 	_SP
cord-014597-66vd2mdu	2439	16	Acknowledgment	Acknowledgment	NNP
cord-014597-66vd2mdu	2439	17	to	to	IN
cord-014597-66vd2mdu	2439	18	Cedric	Cedric	NNP
cord-014597-66vd2mdu	2439	19	Allier	Allier	NNP
cord-014597-66vd2mdu	2439	20	from	from	IN
cord-014597-66vd2mdu	2439	21	CEA	CEA	NNP
cord-014597-66vd2mdu	2439	22	Leti	Leti	NNP
cord-014597-66vd2mdu	2439	23	,	,	,
cord-014597-66vd2mdu	2439	24	Grenoble	Grenoble	NNP
cord-014597-66vd2mdu	2439	25	,	,	,
cord-014597-66vd2mdu	2439	26	France	France	NNP
cord-014597-66vd2mdu	2439	27	.	.	.
cord-014597-66vd2mdu	2440	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2440	2	would	would	MD
cord-014597-66vd2mdu	2440	3	like	like	VB
cord-014597-66vd2mdu	2440	4	to	to	TO
cord-014597-66vd2mdu	2440	5	thank	thank	VB
cord-014597-66vd2mdu	2440	6	A.	A.	NNP
cord-014597-66vd2mdu	2440	7	Schemel	Schemel	NNP
cord-014597-66vd2mdu	2440	8	and	and	CC
cord-014597-66vd2mdu	2440	9	A.	A.	NNP
cord-014597-66vd2mdu	2440	10	Ehrlich	Ehrlich	NNP
cord-014597-66vd2mdu	2440	11	for	for	IN
cord-014597-66vd2mdu	2440	12	technical	technical	JJ
cord-014597-66vd2mdu	2440	13	assistance	assistance	NN
cord-014597-66vd2mdu	2440	14	.	.	.
cord-014597-66vd2mdu	2441	1	The	the	DT
cord-014597-66vd2mdu	2441	2	authors	author	NNS
cord-014597-66vd2mdu	2441	3	would	would	MD
cord-014597-66vd2mdu	2441	4	like	like	VB
cord-014597-66vd2mdu	2441	5	to	to	TO
cord-014597-66vd2mdu	2441	6	mention	mention	VB
cord-014597-66vd2mdu	2441	7	that	that	IN
cord-014597-66vd2mdu	2441	8	this	this	DT
cord-014597-66vd2mdu	2441	9	research	research	NN
cord-014597-66vd2mdu	2441	10	was	be	VBD
cord-014597-66vd2mdu	2441	11	supported	support	VBN
cord-014597-66vd2mdu	2441	12	by	by	IN
cord-014597-66vd2mdu	2441	13	the	the	DT
cord-014597-66vd2mdu	2441	14	FI	FI	NNP
cord-014597-66vd2mdu	2441	15	-	-	HYPH
cord-014597-66vd2mdu	2441	16	DGR	DGR	NNP
cord-014597-66vd2mdu	2441	17	(	(	-LRB-
cord-014597-66vd2mdu	2441	18	2017	2017	CD
cord-014597-66vd2mdu	2441	19	)	)	-RRB-
cord-014597-66vd2mdu	2441	20	from	from	IN
cord-014597-66vd2mdu	2441	21	Spanish	Spanish	NNP
cord-014597-66vd2mdu	2441	22	Government	Government	NNP
cord-014597-66vd2mdu	2441	23	and	and	CC
cord-014597-66vd2mdu	2441	24	the	the	DT
cord-014597-66vd2mdu	2441	25	project	project	NN
cord-014597-66vd2mdu	2441	26	was	be	VBD
cord-014597-66vd2mdu	2441	27	led	lead	VBN
cord-014597-66vd2mdu	2441	28	by	by	IN
cord-014597-66vd2mdu	2441	29	Prof.	Prof.	NNP
cord-014597-66vd2mdu	2441	30	Jordi	Jordi	NNP
cord-014597-66vd2mdu	2441	31	Joan	Joan	NNP
cord-014597-66vd2mdu	2441	32	Cairó	Cairó	NNP
cord-014597-66vd2mdu	2441	33	Badillo	Badillo	NNP
cord-014597-66vd2mdu	2441	34	.	.	.
cord-014597-66vd2mdu	2442	1	The	the	DT
cord-014597-66vd2mdu	2442	2	authors	author	NNS
cord-014597-66vd2mdu	2442	3	want	want	VBP
cord-014597-66vd2mdu	2442	4	to	to	TO
cord-014597-66vd2mdu	2442	5	thank	thank	VB
cord-014597-66vd2mdu	2442	6	the	the	DT
cord-014597-66vd2mdu	2442	7	Biotech	Biotech	NNP
cord-014597-66vd2mdu	2442	8	Process	Process	NNP
cord-014597-66vd2mdu	2442	9	Sciences	Sciences	NNPS
cord-014597-66vd2mdu	2442	10	team	team	NN
cord-014597-66vd2mdu	2442	11	at	at	IN
cord-014597-66vd2mdu	2442	12	Merck	Merck	NNP
cord-014597-66vd2mdu	2442	13	in	in	IN
cord-014597-66vd2mdu	2442	14	Corsier	Corsier	NNP
cord-014597-66vd2mdu	2442	15	-	-	HYPH
cord-014597-66vd2mdu	2442	16	sur	sur	FW
cord-014597-66vd2mdu	2442	17	-	-	HYPH
cord-014597-66vd2mdu	2442	18	Vevey	Vevey	NNP
cord-014597-66vd2mdu	2442	19	for	for	IN
cord-014597-66vd2mdu	2442	20	their	-PRON-	PRP$
cord-014597-66vd2mdu	2442	21	support	support	NN
cord-014597-66vd2mdu	2442	22	and	and	CC
cord-014597-66vd2mdu	2442	23	also	also	RB
cord-014597-66vd2mdu	2442	24	the	the	DT
cord-014597-66vd2mdu	2442	25	members	member	NNS
cord-014597-66vd2mdu	2442	26	of	of	IN
cord-014597-66vd2mdu	2442	27	the	the	DT
cord-014597-66vd2mdu	2442	28	Morbidelli	Morbidelli	NNP
cord-014597-66vd2mdu	2442	29	group	group	NN
cord-014597-66vd2mdu	2442	30	at	at	IN
cord-014597-66vd2mdu	2442	31	ETH	ETH	NNP
cord-014597-66vd2mdu	2442	32	Zürich	Zürich	NNP
cord-014597-66vd2mdu	2442	33	for	for	IN
cord-014597-66vd2mdu	2442	34	their	-PRON-	PRP$
cord-014597-66vd2mdu	2442	35	input	input	NN
cord-014597-66vd2mdu	2442	36	and	and	CC
cord-014597-66vd2mdu	2442	37	collaboration	collaboration	NN
cord-014597-66vd2mdu	2442	38	.	.	.
cord-014597-66vd2mdu	2443	1	Authors	author	NNS
cord-014597-66vd2mdu	2443	2	would	would	MD
cord-014597-66vd2mdu	2443	3	like	like	VB
cord-014597-66vd2mdu	2443	4	to	to	TO
cord-014597-66vd2mdu	2443	5	thank	thank	VB
cord-014597-66vd2mdu	2443	6	Dr.	Dr.	NNP
cord-014597-66vd2mdu	2443	7	Benjamin	Benjamin	NNP
cord-014597-66vd2mdu	2443	8	Youn	Youn	NNP
cord-014597-66vd2mdu	2443	9	in	in	IN
cord-014597-66vd2mdu	2443	10	Manufacturing	Manufacturing	NNP
cord-014597-66vd2mdu	2443	11	Science	Science	NNP
cord-014597-66vd2mdu	2443	12	and	and	CC
cord-014597-66vd2mdu	2443	13	Technology	Technology	NNP
cord-014597-66vd2mdu	2443	14	(	(	-LRB-
cord-014597-66vd2mdu	2443	15	MSAT	MSAT	NNP
cord-014597-66vd2mdu	2443	16	)	)	-RRB-
cord-014597-66vd2mdu	2443	17	at	at	IN
cord-014597-66vd2mdu	2443	18	BioMarin	BioMarin	NNP
cord-014597-66vd2mdu	2443	19	for	for	IN
cord-014597-66vd2mdu	2443	20	his	-PRON-	PRP$
cord-014597-66vd2mdu	2443	21	help	help	NN
cord-014597-66vd2mdu	2443	22	on	on	IN
cord-014597-66vd2mdu	2443	23	coding	code	VBG
cord-014597-66vd2mdu	2443	24	the	the	DT
cord-014597-66vd2mdu	2443	25	Excel	Excel	NNP
cord-014597-66vd2mdu	2443	26	Macro	Macro	NNP
cord-014597-66vd2mdu	2443	27	program	program	NN
cord-014597-66vd2mdu	2443	28	for	for	IN
cord-014597-66vd2mdu	2443	29	AMBR15	AMBR15	NNP
cord-014597-66vd2mdu	2443	30	,	,	,
cord-014597-66vd2mdu	2443	31	and	and	CC
cord-014597-66vd2mdu	2443	32	Dr.	Dr.	NNP
cord-014597-66vd2mdu	2443	33	Donald	Donald	NNP
cord-014597-66vd2mdu	2443	34	L.	L.	NNP
cord-014597-66vd2mdu	2443	35	Traul	Traul	NNP
cord-014597-66vd2mdu	2443	36	from	from	IN
cord-014597-66vd2mdu	2443	37	TAP	TAP	NNP
cord-014597-66vd2mdu	2443	38	Biosystems	Biosystems	NNP
cord-014597-66vd2mdu	2443	39	(	(	-LRB-
cord-014597-66vd2mdu	2443	40	now	now	RB
cord-014597-66vd2mdu	2443	41	part	part	NN
cord-014597-66vd2mdu	2443	42	of	of	IN
cord-014597-66vd2mdu	2443	43	the	the	DT
cord-014597-66vd2mdu	2443	44	Sartorius	Sartorius	NNP
cord-014597-66vd2mdu	2443	45	Stedim	Stedim	NNP
cord-014597-66vd2mdu	2443	46	Biotech	Biotech	NNP
cord-014597-66vd2mdu	2443	47	Group	Group	NNP
cord-014597-66vd2mdu	2443	48	)	)	-RRB-
cord-014597-66vd2mdu	2443	49	for	for	IN
cord-014597-66vd2mdu	2443	50	his	-PRON-	PRP$
cord-014597-66vd2mdu	2443	51	assistance	assistance	NN
cord-014597-66vd2mdu	2443	52	on	on	IN
cord-014597-66vd2mdu	2443	53	AMBR15	AMBR15	NNP
cord-014597-66vd2mdu	2443	54	operations	operation	NNS
cord-014597-66vd2mdu	2443	55	.	.	.
cord-014597-66vd2mdu	2444	1	Thanks	thank	NNS
cord-014597-66vd2mdu	2444	2	to	to	IN
cord-014597-66vd2mdu	2444	3	the	the	DT
cord-014597-66vd2mdu	2444	4	bioreactor	bioreactor	NN
cord-014597-66vd2mdu	2444	5	team	team	NN
cord-014597-66vd2mdu	2444	6	of	of	IN
cord-014597-66vd2mdu	2444	7	Dustin	Dustin	NNP
cord-014597-66vd2mdu	2444	8	Davis	Davis	NNP
cord-014597-66vd2mdu	2444	9	,	,	,
cord-014597-66vd2mdu	2444	10	Amer	Amer	NNP
cord-014597-66vd2mdu	2444	11	Al	Al	NNP
cord-014597-66vd2mdu	2444	12	-	-	HYPH
cord-014597-66vd2mdu	2444	13	Lozi	Lozi	NNP
cord-014597-66vd2mdu	2444	14	,	,	,
cord-014597-66vd2mdu	2444	15	and	and	CC
cord-014597-66vd2mdu	2444	16	Jana	Jana	NNP
cord-014597-66vd2mdu	2444	17	Mahadevan	Mahadevan	NNP
cord-014597-66vd2mdu	2444	18	.	.	.
cord-014597-66vd2mdu	2445	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2445	2	 	 	_SP
cord-014597-66vd2mdu	2445	3	Organic	Organic	NNP
cord-014597-66vd2mdu	2445	4	Vaccines	Vaccines	NNPS
cord-014597-66vd2mdu	2445	5	TM	TM	NNP
cord-014597-66vd2mdu	2445	6	and	and	CC
cord-014597-66vd2mdu	2445	7	the	the	DT
cord-014597-66vd2mdu	2445	8	NIH	NIH	NNP
cord-014597-66vd2mdu	2445	9	,	,	,
cord-014597-66vd2mdu	2445	10	who	who	WP
cord-014597-66vd2mdu	2445	11	kindly	kindly	RB
cord-014597-66vd2mdu	2445	12	provided	provide	VBD
cord-014597-66vd2mdu	2445	13	to	to	IN
cord-014597-66vd2mdu	2445	14	Univercells	Univercells	NNP
cord-014597-66vd2mdu	2445	15	.	.	.
cord-014597-66vd2mdu	2446	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2446	2	thank	thank	VBP
cord-014597-66vd2mdu	2446	3	Polymun	Polymun	NNP
cord-014597-66vd2mdu	2446	4	Scientific	Scientific	NNP
cord-014597-66vd2mdu	2446	5	Immunbiologische	Immunbiologische	NNP
cord-014597-66vd2mdu	2446	6	Forschung	Forschung	NNP
cord-014597-66vd2mdu	2446	7	GmbH	GmbH	NNP
cord-014597-66vd2mdu	2446	8	for	for	IN
cord-014597-66vd2mdu	2446	9	providing	provide	VBG
cord-014597-66vd2mdu	2446	10	the	the	DT
cord-014597-66vd2mdu	2446	11	antibodies	antibody	NNS
cord-014597-66vd2mdu	2446	12	IgM103	IgM103	NNP
cord-014597-66vd2mdu	2446	13	,	,	,
cord-014597-66vd2mdu	2446	14	IgM104	IgM104	NNP
cord-014597-66vd2mdu	2446	15	and	and	CC
cord-014597-66vd2mdu	2446	16	IgM617	IgM617	NNP
cord-014597-66vd2mdu	2446	17	as	as	IN
cord-014597-66vd2mdu	2446	18	a	a	DT
cord-014597-66vd2mdu	2446	19	kind	kind	JJ
cord-014597-66vd2mdu	2446	20	gift	gift	NN
cord-014597-66vd2mdu	2446	21	.	.	.
cord-014597-66vd2mdu	2447	1	This	this	DT
cord-014597-66vd2mdu	2447	2	Allison	Allison	NNP
cord-014597-66vd2mdu	2447	3	Kurz	Kurz	NNP
cord-014597-66vd2mdu	2447	4	and	and	CC
cord-014597-66vd2mdu	2447	5	Gian	Gian	NNP
cord-014597-66vd2mdu	2447	6	Andrea	Andrea	NNP
cord-014597-66vd2mdu	2447	7	Signorell	Signorell	NNP
cord-014597-66vd2mdu	2447	8	,	,	,
cord-014597-66vd2mdu	2447	9	Genedata	Genedata	NNP
cord-014597-66vd2mdu	2447	10	AG	AG	NNP
cord-014597-66vd2mdu	2447	11	,	,	,
cord-014597-66vd2mdu	2447	12	Basel	Basel	NNP
cord-014597-66vd2mdu	2447	13	,	,	,
cord-014597-66vd2mdu	2447	14	Switzerland	Switzerland	NNP
cord-014597-66vd2mdu	2447	15	Allison	Allison	NNP
cord-014597-66vd2mdu	2447	16	Kurz	Kurz	NNP
cord-014597-66vd2mdu	2447	17	,	,	,
cord-014597-66vd2mdu	2447	18	Gian	Gian	NNP
cord-014597-66vd2mdu	2447	19	Andrea	Andrea	NNP
cord-014597-66vd2mdu	2447	20	Signorell	Signorell	NNP
cord-014597-66vd2mdu	2447	21	,	,	,
cord-014597-66vd2mdu	2447	22	Genedata	Genedata	NNP
cord-014597-66vd2mdu	2447	23	,	,	,
cord-014597-66vd2mdu	2447	24	Basel	Basel	NNP
cord-014597-66vd2mdu	2447	25	,	,	,
cord-014597-66vd2mdu	2447	26	Switzerland	Switzerland	NNP
cord-014597-66vd2mdu	2447	27	.	.	.
cord-014597-66vd2mdu	2448	1	Allison	Allison	NNP
cord-014597-66vd2mdu	2448	2	Kurz	Kurz	NNP
cord-014597-66vd2mdu	2448	3	,	,	,
cord-014597-66vd2mdu	2448	4	Gian	Gian	NNP
cord-014597-66vd2mdu	2448	5	Andrea	Andrea	NNP
cord-014597-66vd2mdu	2448	6	Signorell	Signorell	NNP
cord-014597-66vd2mdu	2448	7	,	,	,
cord-014597-66vd2mdu	2448	8	Genedata	Genedata	NNP
cord-014597-66vd2mdu	2448	9	AG	AG	NNP
cord-014597-66vd2mdu	2448	10	High	high	JJ
cord-014597-66vd2mdu	2448	11	glucose	glucose	NN
cord-014597-66vd2mdu	2448	12	concentration	concentration	NN
cord-014597-66vd2mdu	2448	13	and	and	CC
cord-014597-66vd2mdu	2448	14	low	low	JJ
cord-014597-66vd2mdu	2448	15	specific	specific	JJ
cord-014597-66vd2mdu	2448	16	cell	cell	NN
cord-014597-66vd2mdu	2448	17	growth	growth	NN
cord-014597-66vd2mdu	2448	18	rate	rate	NN
cord-014597-66vd2mdu	2448	19	improve	improve	VBP
cord-014597-66vd2mdu	2448	20	specific	specific	JJ
cord-014597-66vd2mdu	2448	21	r	r	NN
cord-014597-66vd2mdu	2448	22	-	-	HYPH
cord-014597-66vd2mdu	2448	23	tPA	tpa	NN
cord-014597-66vd2mdu	2448	24	productivity	productivity	NN
cord-014597-66vd2mdu	2448	25	in	in	IN
cord-014597-66vd2mdu	2448	26	chemostat	chemostat	NN
cord-014597-66vd2mdu	2448	27	culture	culture	NN
cord-014597-66vd2mdu	2448	28	of	of	IN
cord-014597-66vd2mdu	2448	29	CHO	CHO	NNP
cord-014597-66vd2mdu	2448	30	Acknowledgements	Acknowledgements	NNP
cord-014597-66vd2mdu	2448	31	R.	R.	NNP
cord-014597-66vd2mdu	2448	32	Boraston	Boraston	NNP
cord-014597-66vd2mdu	2448	33	for	for	IN
cord-014597-66vd2mdu	2448	34	photographs	photograph	NNS
cord-014597-66vd2mdu	2448	35	in	in	IN
cord-014597-66vd2mdu	2448	36	Fig	Fig	NNP
cord-014597-66vd2mdu	2448	37	.	.	.
cord-014597-66vd2mdu	2449	1	1	1	LS
cord-014597-66vd2mdu	2449	2	.	.	.
cord-014597-66vd2mdu	2450	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2450	2	acknowledge	acknowledge	VBP
cord-014597-66vd2mdu	2450	3	Atum	Atum	NNP
cord-014597-66vd2mdu	2450	4	for	for	IN
cord-014597-66vd2mdu	2450	5	their	-PRON-	PRP$
cord-014597-66vd2mdu	2450	6	contributions	contribution	NNS
cord-014597-66vd2mdu	2450	7	to	to	IN
cord-014597-66vd2mdu	2450	8	vector	vector	NN
cord-014597-66vd2mdu	2450	9	design	design	NN
cord-014597-66vd2mdu	2450	10	and	and	CC
cord-014597-66vd2mdu	2450	11	construction	construction	NN
cord-014597-66vd2mdu	2450	12	.	.	.
cord-014597-66vd2mdu	2451	1	Austrian	Austrian	NNP
cord-014597-66vd2mdu	2451	2	BMWFW	BMWFW	NNP
cord-014597-66vd2mdu	2451	3	,	,	,
cord-014597-66vd2mdu	2451	4	BMVIT	BMVIT	NNP
cord-014597-66vd2mdu	2451	5	,	,	,
cord-014597-66vd2mdu	2451	6	SFG	SFG	NNP
cord-014597-66vd2mdu	2451	7	,	,	,
cord-014597-66vd2mdu	2451	8	Standortagentur	Standortagentur	NNP
cord-014597-66vd2mdu	2451	9	Tirol	Tirol	NNP
cord-014597-66vd2mdu	2451	10	,	,	,
cord-014597-66vd2mdu	2451	11	Government	Government	NNP
cord-014597-66vd2mdu	2451	12	of	of	IN
cord-014597-66vd2mdu	2451	13	Lower	Lower	NNP
cord-014597-66vd2mdu	2451	14	Austria	Austria	NNP
cord-014597-66vd2mdu	2451	15	and	and	CC
cord-014597-66vd2mdu	2451	16	Business	Business	NNP
cord-014597-66vd2mdu	2451	17	Agency	Agency	NNP
cord-014597-66vd2mdu	2451	18	Vienna	Vienna	NNP
cord-014597-66vd2mdu	2451	19	through	through	IN
cord-014597-66vd2mdu	2451	20	the	the	DT
cord-014597-66vd2mdu	2451	21	Austrian	austrian	JJ
cord-014597-66vd2mdu	2451	22	FFG	FFG	NNP
cord-014597-66vd2mdu	2451	23	-	-	HYPH
cord-014597-66vd2mdu	2451	24	COMET	COMET	NNP
cord-014597-66vd2mdu	2451	25	-	-	HYPH
cord-014597-66vd2mdu	2451	26	K2	K2	NNP
cord-014597-66vd2mdu	2451	27	.	.	.
cord-014597-66vd2mdu	2452	1	This	this	DT
cord-014597-66vd2mdu	2452	2	research	research	NN
cord-014597-66vd2mdu	2452	3	is	be	VBZ
cord-014597-66vd2mdu	2452	4	supported	support	VBN
cord-014597-66vd2mdu	2452	5	by	by	IN
cord-014597-66vd2mdu	2452	6	SFI	SFI	NNP
cord-014597-66vd2mdu	2452	7	Grant	Grant	NNP
cord-014597-66vd2mdu	2452	8	13	13	CD
cord-014597-66vd2mdu	2453	1	/IA/1841	/IA/1841	NFP
cord-014597-66vd2mdu	2453	2	.	.	.
cord-014597-66vd2mdu	2453	3	 	 	_SP
cord-014597-66vd2mdu	2454	1	Financial	financial	JJ
cord-014597-66vd2mdu	2454	2	support	support	NN
cord-014597-66vd2mdu	2454	3	of	of	IN
cord-014597-66vd2mdu	2454	4	the	the	DT
cord-014597-66vd2mdu	2454	5	Austrian	Austrian	NNP
cord-014597-66vd2mdu	2454	6	Science	Science	NNP
cord-014597-66vd2mdu	2454	7	Fund	Fund	NNP
cord-014597-66vd2mdu	2454	8	(	(	-LRB-
cord-014597-66vd2mdu	2454	9	FWF	FWF	NNP
cord-014597-66vd2mdu	2454	10	;	;	,
cord-014597-66vd2mdu	2454	11	grant	grant	NN
cord-014597-66vd2mdu	2454	12	number	number	NN
cord-014597-66vd2mdu	2454	13	P	p	NN
cord-014597-66vd2mdu	2454	14	25056	25056	CD
cord-014597-66vd2mdu	2454	15	)	)	-RRB-
cord-014597-66vd2mdu	2454	16	is	be	VBZ
cord-014597-66vd2mdu	2454	17	gratefully	gratefully	RB
cord-014597-66vd2mdu	2454	18	acknowledged	acknowledge	VBN
cord-014597-66vd2mdu	2454	19	.	.	.
cord-014597-66vd2mdu	2455	1	We	-PRON-	PRP
cord-014597-66vd2mdu	2455	2	would	would	MD
cord-014597-66vd2mdu	2455	3	like	like	VB
cord-014597-66vd2mdu	2455	4	to	to	TO
cord-014597-66vd2mdu	2455	5	thank	thank	VB
cord-014597-66vd2mdu	2455	6	the	the	DT
cord-014597-66vd2mdu	2455	7	Australian	Australian	NNP
cord-014597-66vd2mdu	2455	8	Institute	Institute	NNP
cord-014597-66vd2mdu	2455	9	for	for	IN
cord-014597-66vd2mdu	2455	10	Bioengineering	Bioengineering	NNP
cord-014597-66vd2mdu	2455	11	and	and	CC
cord-014597-66vd2mdu	2455	12	Nanotechnology	Nanotechnology	NNP
cord-014597-66vd2mdu	2455	13	,	,	,
cord-014597-66vd2mdu	2455	14	University	University	NNP
cord-014597-66vd2mdu	2455	15	of	of	IN
cord-014597-66vd2mdu	2455	16	Queensland	Queensland	NNP
cord-014597-66vd2mdu	2455	17	-	-	HYPH
cord-014597-66vd2mdu	2455	18	Brisbane	Brisbane	NNP
cord-014597-66vd2mdu	2455	19	,	,	,
cord-014597-66vd2mdu	2455	20	Australia	Australia	NNP
cord-014597-66vd2mdu	2455	21	(	(	-LRB-
cord-014597-66vd2mdu	2455	22	AIBN	AIBN	NNP
cord-014597-66vd2mdu	2455	23	)	)	-RRB-
cord-014597-66vd2mdu	2455	24	for	for	IN
cord-014597-66vd2mdu	2455	25	providing	provide	VBG
cord-014597-66vd2mdu	2455	26	the	the	DT
cord-014597-66vd2mdu	2455	27	CHO	CHO	NNP
cord-014597-66vd2mdu	2455	28	clones	clone	NNS
cord-014597-66vd2mdu	2455	29	.	.	.
cord-014597-66vd2mdu	2456	1	This	this	DT
cord-014597-66vd2mdu	2456	2	research	research	NN
cord-014597-66vd2mdu	2456	3	is	be	VBZ
cord-014597-66vd2mdu	2456	4	partially	partially	RB
cord-014597-66vd2mdu	2456	5	supported	support	VBN
cord-014597-66vd2mdu	2456	6	by	by	IN
cord-014597-66vd2mdu	2456	7	the	the	DT
cord-014597-66vd2mdu	2456	8	developing	develop	VBG
cord-014597-66vd2mdu	2456	9	key	key	JJ
cord-014597-66vd2mdu	2456	10	technologies	technology	NNS
cord-014597-66vd2mdu	2456	11	for	for	IN
cord-014597-66vd2mdu	2456	12	discovering	discover	VBG
cord-014597-66vd2mdu	2456	13	and	and	CC
cord-014597-66vd2mdu	2456	14	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	2456	15	pharmaceuticals	pharmaceutical	NNS
cord-014597-66vd2mdu	2456	16	used	use	VBN
cord-014597-66vd2mdu	2456	17	for	for	IN
cord-014597-66vd2mdu	2456	18	next	next	JJ
cord-014597-66vd2mdu	2456	19	-	-	HYPH
cord-014597-66vd2mdu	2456	20	generation	generation	NN
cord-014597-66vd2mdu	2456	21	treatments	treatment	NNS
cord-014597-66vd2mdu	2456	22	and	and	CC
cord-014597-66vd2mdu	2456	23	diagnoses	diagnosis	NNS
cord-014597-66vd2mdu	2456	24	both	both	DT
cord-014597-66vd2mdu	2456	25	from	from	IN
cord-014597-66vd2mdu	2456	26	METI	METI	NNP
cord-014597-66vd2mdu	2456	27	and	and	CC
cord-014597-66vd2mdu	2456	28	AMED	AMED	NNP
cord-014597-66vd2mdu	2456	29	,	,	,
cord-014597-66vd2mdu	2456	30	Japan	Japan	NNP
cord-014597-66vd2mdu	2456	31	.	.	.
cord-014597-66vd2mdu	2456	32	 	 	_SP
cord-014597-66vd2mdu	2457	1	This	this	DT
cord-014597-66vd2mdu	2457	2	work	work	NN
cord-014597-66vd2mdu	2457	3	was	be	VBD
cord-014597-66vd2mdu	2457	4	supported	support	VBN
cord-014597-66vd2mdu	2457	5	in	in	IN
cord-014597-66vd2mdu	2457	6	part	part	NN
cord-014597-66vd2mdu	2457	7	by	by	IN
cord-014597-66vd2mdu	2457	8	grants	grant	NNS
cord-014597-66vd2mdu	2457	9	for	for	IN
cord-014597-66vd2mdu	2457	10	developing	develop	VBG
cord-014597-66vd2mdu	2457	11	key	key	JJ
cord-014597-66vd2mdu	2457	12	technologies	technology	NNS
cord-014597-66vd2mdu	2457	13	for	for	IN
cord-014597-66vd2mdu	2457	14	discovering	discover	VBG
cord-014597-66vd2mdu	2457	15	and	and	CC
cord-014597-66vd2mdu	2457	16	manufacturing	manufacturing	NN
cord-014597-66vd2mdu	2457	17	pharmaceuticals	pharmaceutical	NNS
cord-014597-66vd2mdu	2457	18	used	use	VBN
cord-014597-66vd2mdu	2457	19	for	for	IN
cord-014597-66vd2mdu	2457	20	next	next	JJ
cord-014597-66vd2mdu	2457	21	-	-	HYPH
cord-014597-66vd2mdu	2457	22	generation	generation	NN
cord-014597-66vd2mdu	2457	23	treatment	treatment	NN
cord-014597-66vd2mdu	2457	24	and	and	CC
cord-014597-66vd2mdu	2457	25	diagnoses	diagnosis	NNS
cord-014597-66vd2mdu	2457	26	,	,	,
cord-014597-66vd2mdu	2457	27	both	both	CC
cord-014597-66vd2mdu	2457	28	from	from	IN
cord-014597-66vd2mdu	2457	29	the	the	DT
cord-014597-66vd2mdu	2457	30	Ministry	Ministry	NNP
cord-014597-66vd2mdu	2457	31	of	of	IN
cord-014597-66vd2mdu	2457	32	Economy	Economy	NNP
cord-014597-66vd2mdu	2457	33	,	,	,
cord-014597-66vd2mdu	2457	34	Trade	Trade	NNP
cord-014597-66vd2mdu	2457	35	and	and	CC
cord-014597-66vd2mdu	2457	36	Industry	Industry	NNP
cord-014597-66vd2mdu	2457	37	(	(	-LRB-
cord-014597-66vd2mdu	2457	38	METI	METI	NNP
cord-014597-66vd2mdu	2457	39	)	)	-RRB-
cord-014597-66vd2mdu	2457	40	,	,	,
cord-014597-66vd2mdu	2457	41	Japan	Japan	NNP
cord-014597-66vd2mdu	2457	42	and	and	CC
cord-014597-66vd2mdu	2457	43	from	from	IN
cord-014597-66vd2mdu	2457	44	the	the	DT
cord-014597-66vd2mdu	2457	45	Japan	Japan	NNP
cord-014597-66vd2mdu	2457	46	Agency	Agency	NNP
cord-014597-66vd2mdu	2457	47	for	for	IN
cord-014597-66vd2mdu	2457	48	Medical	Medical	NNP
cord-014597-66vd2mdu	2457	49	Research	Research	NNP
cord-014597-66vd2mdu	2457	50	and	and	CC
cord-014597-66vd2mdu	2457	51	Developments	Developments	NNPS
cord-014597-66vd2mdu	2457	52	(	(	-LRB-
cord-014597-66vd2mdu	2457	53	AMED	AMED	NNP
cord-014597-66vd2mdu	2457	54	)	)	-RRB-
cord-014597-66vd2mdu	2457	55	.	.	.
cord-014597-66vd2mdu	2458	1	Many	many	JJ
cord-014597-66vd2mdu	2458	2	thanks	thank	NNS
cord-014597-66vd2mdu	2458	3	Stefanos	Stefanos	NNP
cord-014597-66vd2mdu	2458	4	Grammatikos	Grammatikos	NNP
cord-014597-66vd2mdu	2458	5	for	for	IN
cord-014597-66vd2mdu	2458	6	his	-PRON-	PRP$
cord-014597-66vd2mdu	2458	7	support	support	NN
cord-014597-66vd2mdu	2458	8	and	and	CC
cord-014597-66vd2mdu	2458	9	to	to	IN
cord-014597-66vd2mdu	2458	10	the	the	DT
cord-014597-66vd2mdu	2458	11	whole	whole	JJ
cord-014597-66vd2mdu	2458	12	Upstream	Upstream	NNP
cord-014597-66vd2mdu	2458	13	Process	Process	NNP
cord-014597-66vd2mdu	2458	14	Sciences	Sciences	NNPS
cord-014597-66vd2mdu	2458	15	team	team	NN
cord-014597-66vd2mdu	2458	16	.	.	.
cord-014597-66vd2mdu	2459	1	The	the	DT
cord-014597-66vd2mdu	2459	2	authors	author	NNS
cord-014597-66vd2mdu	2459	3	thank	thank	VBP
cord-014597-66vd2mdu	2459	4	the	the	DT
cord-014597-66vd2mdu	2459	5	Hessen	Hessen	NNP
cord-014597-66vd2mdu	2459	6	State	State	NNP
cord-014597-66vd2mdu	2459	7	Ministry	Ministry	NNP
cord-014597-66vd2mdu	2459	8	of	of	IN
cord-014597-66vd2mdu	2459	9	Higher	Higher	NNP
cord-014597-66vd2mdu	2459	10	Education	Education	NNP
cord-014597-66vd2mdu	2459	11	,	,	,
cord-014597-66vd2mdu	2459	12	Research	Research	NNP
cord-014597-66vd2mdu	2459	13	and	and	CC
cord-014597-66vd2mdu	2459	14	the	the	DT
cord-014597-66vd2mdu	2459	15	Arts	art	NNS
cord-014597-66vd2mdu	2459	16	within	within	IN
cord-014597-66vd2mdu	2459	17	the	the	DT
cord-014597-66vd2mdu	2459	18	Hessen	Hessen	NNP
cord-014597-66vd2mdu	2459	19	initiative	initiative	NN
cord-014597-66vd2mdu	2459	20	for	for	IN
cord-014597-66vd2mdu	2459	21	scientific	scientific	JJ
cord-014597-66vd2mdu	2459	22	and	and	CC
cord-014597-66vd2mdu	2459	23	economic	economic	JJ
cord-014597-66vd2mdu	2459	24	excellence	excellence	NN
cord-014597-66vd2mdu	2459	25	(	(	-LRB-
cord-014597-66vd2mdu	2459	26	LOEWE	LOEWE	NNP
cord-014597-66vd2mdu	2459	27	Program	Program	NNP
cord-014597-66vd2mdu	2459	28	)	)	-RRB-
cord-014597-66vd2mdu	2459	29	for	for	IN
cord-014597-66vd2mdu	2459	30	the	the	DT
cord-014597-66vd2mdu	2459	31	financial	financial	JJ
cord-014597-66vd2mdu	2459	32	support	support	NN
cord-014597-66vd2mdu	2459	33	.	.	.
cord-014597-66vd2mdu	2459	34	 	 	_SP
cord-014597-66vd2mdu	2460	1	The	the	DT
cord-014597-66vd2mdu	2460	2	authors	author	NNS
cord-014597-66vd2mdu	2460	3	thank	thank	VBP
cord-014597-66vd2mdu	2460	4	Xell	Xell	NNP
cord-014597-66vd2mdu	2460	5	AG	AG	NNP
cord-014597-66vd2mdu	2460	6	,	,	,
cord-014597-66vd2mdu	2460	7	Bielefeld	Bielefeld	NNP
cord-014597-66vd2mdu	2460	8	,	,	,
cord-014597-66vd2mdu	2460	9	for	for	IN
cord-014597-66vd2mdu	2460	10	providing	provide	VBG
cord-014597-66vd2mdu	2460	11	HEK	HEK	NNP
cord-014597-66vd2mdu	2460	12	serum	serum	NN
cord-014597-66vd2mdu	2460	13	-	-	HYPH
cord-014597-66vd2mdu	2460	14	free	free	JJ
cord-014597-66vd2mdu	2460	15	media	medium	NNS
cord-014597-66vd2mdu	2460	16	(	(	-LRB-
cord-014597-66vd2mdu	2460	17	HEK	HEK	NNP
cord-014597-66vd2mdu	2460	18	GM	GM	NNP
cord-014597-66vd2mdu	2460	19	and	and	CC
cord-014597-66vd2mdu	2460	20	HEK	HEK	NNP
cord-014597-66vd2mdu	2460	21	TF	TF	NNP
cord-014597-66vd2mdu	2460	22	)	)	-RRB-
cord-014597-66vd2mdu	2460	23	and	and	CC
cord-014597-66vd2mdu	2460	24	for	for	IN
cord-014597-66vd2mdu	2460	25	fruitful	fruitful	JJ
cord-014597-66vd2mdu	2460	26	discussions	discussion	NNS
cord-014597-66vd2mdu	2460	27	.	.	.
cord-014597-66vd2mdu	2461	1	The	the	DT
cord-014597-66vd2mdu	2461	2	authors	author	NNS
cord-014597-66vd2mdu	2461	3	would	would	MD
cord-014597-66vd2mdu	2461	4	like	like	VB
cord-014597-66vd2mdu	2461	5	to	to	TO
cord-014597-66vd2mdu	2461	6	thank	thank	VB
cord-014597-66vd2mdu	2461	7	Dana	Dana	NNP
cord-014597-66vd2mdu	2461	8	Wenzel	Wenzel	NNP
cord-014597-66vd2mdu	2461	9	for	for	IN
cord-014597-66vd2mdu	2461	10	CHO	CHO	NNP
cord-014597-66vd2mdu	2461	11	lysate	lysate	NN
cord-014597-66vd2mdu	2461	12	preparation	preparation	NN
cord-014597-66vd2mdu	2461	13	(	(	-LRB-
cord-014597-66vd2mdu	2461	14	Fraunhofer	Fraunhofer	NNP
cord-014597-66vd2mdu	2461	15	IZI	IZI	NNP
cord-014597-66vd2mdu	2461	16	,	,	,
cord-014597-66vd2mdu	2461	17	Potsdam	Potsdam	NNP
cord-014597-66vd2mdu	2461	18	-	-	HYPH
cord-014597-66vd2mdu	2461	19	Golm	Golm	NNP
cord-014597-66vd2mdu	2461	20	,	,	,
cord-014597-66vd2mdu	2461	21	Germany	Germany	NNP
cord-014597-66vd2mdu	2461	22	)	)	-RRB-
cord-014597-66vd2mdu	2461	23	.	.	.
cord-014597-66vd2mdu	2462	1	This	this	DT
cord-014597-66vd2mdu	2462	2	work	work	NN
cord-014597-66vd2mdu	2462	3	is	be	VBZ
cord-014597-66vd2mdu	2462	4	supported	support	VBN
cord-014597-66vd2mdu	2462	5	by	by	IN
cord-014597-66vd2mdu	2462	6	the	the	DT
cord-014597-66vd2mdu	2462	7	European	European	NNP
cord-014597-66vd2mdu	2462	8	Regional	Regional	NNP
cord-014597-66vd2mdu	2462	9	Development	Development	NNP
cord-014597-66vd2mdu	2462	10	Fund	Fund	NNP
cord-014597-66vd2mdu	2462	11	(	(	-LRB-
cord-014597-66vd2mdu	2462	12	EFRE	EFRE	NNP
cord-014597-66vd2mdu	2462	13	)	)	-RRB-
cord-014597-66vd2mdu	2462	14	and	and	CC
cord-014597-66vd2mdu	2462	15	the	the	DT
cord-014597-66vd2mdu	2462	16	German	German	NNP
cord-014597-66vd2mdu	2462	17	Ministry	Ministry	NNP
cord-014597-66vd2mdu	2462	18	of	of	IN
cord-014597-66vd2mdu	2462	19	Education	Education	NNP
cord-014597-66vd2mdu	2462	20	and	and	CC
cord-014597-66vd2mdu	2462	21	Research	Research	NNP
cord-014597-66vd2mdu	2462	22	(	(	-LRB-
cord-014597-66vd2mdu	2462	23	BMBF	BMBF	NNP
cord-014597-66vd2mdu	2462	24	,	,	,
cord-014597-66vd2mdu	2462	25	No	no	NN
cord-014597-66vd2mdu	2462	26	.	.	NN
cord-014597-66vd2mdu	2462	27	031B0078A	031b0078a	CD
cord-014597-66vd2mdu	2462	28	)	)	-RRB-
cord-014597-66vd2mdu	2462	29	.	.	.
cord-014597-66vd2mdu	2463	1	The	the	DT
cord-014597-66vd2mdu	2463	2	authors	author	NNS
cord-014597-66vd2mdu	2463	3	acknowledge	acknowledge	VBP
cord-014597-66vd2mdu	2463	4	São	São	NNP
cord-014597-66vd2mdu	2463	5	Paulo	Paulo	NNP
cord-014597-66vd2mdu	2463	6	Research	Research	NNP
cord-014597-66vd2mdu	2463	7	Foundation	Foundation	NNP
cord-014597-66vd2mdu	2463	8	-FAPESP	-FAPESP	.
cord-014597-66vd2mdu	2464	1	(	(	-LRB-
cord-014597-66vd2mdu	2464	2	2015/	2015/	CD
cord-014597-66vd2mdu	2464	3	19017	19017	CD
cord-014597-66vd2mdu	2464	4	-	-	SYM
cord-014597-66vd2mdu	2464	5	6	6	CD
cord-014597-66vd2mdu	2464	6	)	)	-RRB-
cord-014597-66vd2mdu	2464	7	,	,	,
cord-014597-66vd2mdu	2464	8	Centro	Centro	NNP
cord-014597-66vd2mdu	2464	9	de	de	NNP
cord-014597-66vd2mdu	2464	10	Pesquisa	Pesquisa	NNP
cord-014597-66vd2mdu	2464	11	,	,	,
cord-014597-66vd2mdu	2464	12	Inovação	Inovação	NNP
cord-014597-66vd2mdu	2464	13	e	e	NN
cord-014597-66vd2mdu	2464	14	Difusão	Difusão	NNP
cord-014597-66vd2mdu	2464	15	(	(	-LRB-
cord-014597-66vd2mdu	2464	16	CEPID	CEPID	NNP
cord-014597-66vd2mdu	2464	17	)	)	-RRB-
cord-014597-66vd2mdu	2464	18	,	,	,
cord-014597-66vd2mdu	2464	19	and	and	CC
cord-014597-66vd2mdu	2464	20	National	National	NNP
cord-014597-66vd2mdu	2464	21	Institute	Institute	NNP
cord-014597-66vd2mdu	2464	22	of	of	IN
cord-014597-66vd2mdu	2464	23	Science	Science	NNP
cord-014597-66vd2mdu	2464	24	and	and	CC
cord-014597-66vd2mdu	2464	25	Technology	Technology	NNP
cord-014597-66vd2mdu	2464	26	in	in	IN
cord-014597-66vd2mdu	2464	27	Stem	Stem	NNP
cord-014597-66vd2mdu	2464	28	Cell	Cell	NNP
cord-014597-66vd2mdu	2464	29	and	and	CC
cord-014597-66vd2mdu	2464	30	Cell	Cell	NNP
cord-014597-66vd2mdu	2464	31	Therapy	Therapy	NNP
cord-014597-66vd2mdu	2464	32	-INCTC	-INCTC	.
cord-014597-66vd2mdu	2464	33	for	for	IN
cord-014597-66vd2mdu	2464	34	financial	financial	JJ
cord-014597-66vd2mdu	2464	35	support	support	NN
cord-014597-66vd2mdu	2464	36	.	.	.
cord-014597-66vd2mdu	2465	1	This	this	DT
cord-014597-66vd2mdu	2465	2	work	work	NN
cord-014597-66vd2mdu	2465	3	was	be	VBD
cord-014597-66vd2mdu	2465	4	supported	support	VBN
cord-014597-66vd2mdu	2465	5	by	by	IN
cord-014597-66vd2mdu	2465	6	grants	grant	NNS
cord-014597-66vd2mdu	2465	7	from	from	IN
cord-014597-66vd2mdu	2465	8	the	the	DT
cord-014597-66vd2mdu	2465	9	Niedersächsisches	Niedersächsisches	NNP
cord-014597-66vd2mdu	2465	10	Ministerium	Ministerium	NNP
cord-014597-66vd2mdu	2465	11	für	für	IN
cord-014597-66vd2mdu	2465	12	Wissenschaft	Wissenschaft	NNP
cord-014597-66vd2mdu	2466	1	und	und	IN
cord-014597-66vd2mdu	2466	2	Kultur	Kultur	NNP
cord-014597-66vd2mdu	2466	3	(	(	-LRB-
cord-014597-66vd2mdu	2466	4	80029155	80029155	CD
cord-014597-66vd2mdu	2466	5	)	)	-RRB-
cord-014597-66vd2mdu	2466	6	and	and	CC
cord-014597-66vd2mdu	2466	7	the	the	DT
cord-014597-66vd2mdu	2466	8	German	German	NNP
cord-014597-66vd2mdu	2466	9	Ministry	Ministry	NNP
cord-014597-66vd2mdu	2466	10	for	for	IN
cord-014597-66vd2mdu	2466	11	Economic	Economic	NNP
cord-014597-66vd2mdu	2466	12	Affairs	Affairs	NNPS
cord-014597-66vd2mdu	2466	13	and	and	CC
cord-014597-66vd2mdu	2466	14	Energy	Energy	NNP
cord-014597-66vd2mdu	2466	15	(	(	-LRB-
cord-014597-66vd2mdu	2466	16	IGF	IGF	NNP
cord-014597-66vd2mdu	2466	17	16153	16153	CD
cord-014597-66vd2mdu	2466	18	N	n	CD
cord-014597-66vd2mdu	2466	19	)	)	-RRB-
cord-014597-66vd2mdu	2466	20	.	.	.
cord-014597-66vd2mdu	2467	1	The	the	DT
cord-014597-66vd2mdu	2467	2	infrastructure	infrastructure	NN
cord-014597-66vd2mdu	2467	3	which	which	WDT
cord-014597-66vd2mdu	2467	4	was	be	VBD
cord-014597-66vd2mdu	2467	5	modularly	modularly	RB
cord-014597-66vd2mdu	2467	6	built	build	VBN
cord-014597-66vd2mdu	2467	7	up	up	RP
cord-014597-66vd2mdu	2467	8	,	,	,
cord-014597-66vd2mdu	2467	9	consists	consist	VBZ
cord-014597-66vd2mdu	2467	10	of	of	IN
cord-014597-66vd2mdu	2467	11	automated	automate	VBN
cord-014597-66vd2mdu	2467	12	liquid	liquid	NN
cord-014597-66vd2mdu	2467	13	handling	handling	NN
cord-014597-66vd2mdu	2467	14	robots	robot	NNS
cord-014597-66vd2mdu	2467	15	,	,	,
cord-014597-66vd2mdu	2467	16	plate	plate	NN
cord-014597-66vd2mdu	2467	17	and	and	CC
cord-014597-66vd2mdu	2467	18	tube	tube	NN
cord-014597-66vd2mdu	2467	19	handling	handle	VBG
cord-014597-66vd2mdu	2467	20	robots	robot	NNS
cord-014597-66vd2mdu	2467	21	as	as	RB
cord-014597-66vd2mdu	2467	22	well	well	RB
cord-014597-66vd2mdu	2467	23	as	as	IN
cord-014597-66vd2mdu	2467	24	incubators	incubator	NNS
cord-014597-66vd2mdu	2467	25	,	,	,
cord-014597-66vd2mdu	2467	26	refrigerator	refrigerator	NN
cord-014597-66vd2mdu	2467	27	and	and	CC
cord-014597-66vd2mdu	2467	28	analysis	analysis	NN
cord-014597-66vd2mdu	2467	29	systems	system	NNS
cord-014597-66vd2mdu	2467	30	as	as	IN
cord-014597-66vd2mdu	2467	31	for	for	IN
cord-014597-66vd2mdu	2467	32	example	example	NN
cord-014597-66vd2mdu	2467	33	an	an	DT
cord-014597-66vd2mdu	2467	34	imaging	imaging	NN
cord-014597-66vd2mdu	2467	35	system	system	NN
cord-014597-66vd2mdu	2467	36	.	.	.
cord-014597-66vd2mdu	2468	1	The	the	DT
cord-014597-66vd2mdu	2468	2	aim	aim	NN
cord-014597-66vd2mdu	2468	3	is	be	VBZ
cord-014597-66vd2mdu	2468	4	to	to	TO
cord-014597-66vd2mdu	2468	5	address	address	VB
cord-014597-66vd2mdu	2468	6	the	the	DT
cord-014597-66vd2mdu	2468	7	need	need	NN
cord-014597-66vd2mdu	2468	8	on	on	IN
cord-014597-66vd2mdu	2468	9	reproducibility	reproducibility	NN
cord-014597-66vd2mdu	2468	10	and	and	CC
cord-014597-66vd2mdu	2468	11	reliability	reliability	NN
cord-014597-66vd2mdu	2468	12	of	of	IN
cord-014597-66vd2mdu	2468	13	results	result	NNS
cord-014597-66vd2mdu	2468	14	and	and	CC
cord-014597-66vd2mdu	2468	15	to	to	TO
cord-014597-66vd2mdu	2468	16	offer	offer	VB
cord-014597-66vd2mdu	2468	17	access	access	NN
cord-014597-66vd2mdu	2468	18	to	to	IN
cord-014597-66vd2mdu	2468	19	a	a	DT
cord-014597-66vd2mdu	2468	20	maximal	maximal	RB
cord-014597-66vd2mdu	2468	21	controlled	control	VBN
cord-014597-66vd2mdu	2468	22	and	and	CC
cord-014597-66vd2mdu	2468	23	automated	automate	VBN
cord-014597-66vd2mdu	2468	24	environment	environment	NN
cord-014597-66vd2mdu	2468	25	.	.	.
cord-014597-66vd2mdu	2469	1	With	with	IN
cord-014597-66vd2mdu	2469	2	the	the	DT
cord-014597-66vd2mdu	2469	3	help	help	NN
cord-014597-66vd2mdu	2469	4	of	of	IN
cord-014597-66vd2mdu	2469	5	a	a	DT
cord-014597-66vd2mdu	2469	6	web	web	NN
cord-014597-66vd2mdu	2469	7	-	-	HYPH
cord-014597-66vd2mdu	2469	8	based	base	VBN
cord-014597-66vd2mdu	2469	9	configurator	configurator	NN
cord-014597-66vd2mdu	2469	10	assay	assay	NN
cord-014597-66vd2mdu	2469	11	selection	selection	NN
cord-014597-66vd2mdu	2469	12	as	as	RB
cord-014597-66vd2mdu	2469	13	well	well	RB
cord-014597-66vd2mdu	2469	14	as	as	IN
cord-014597-66vd2mdu	2469	15	parameterization	parameterization	NN
cord-014597-66vd2mdu	2469	16	of	of	IN
cord-014597-66vd2mdu	2469	17	the	the	DT
cord-014597-66vd2mdu	2469	18	assays	assay	NNS
cord-014597-66vd2mdu	2469	19	can	can	MD
cord-014597-66vd2mdu	2469	20	be	be	VB
cord-014597-66vd2mdu	2469	21	done	do	VBN
cord-014597-66vd2mdu	2469	22	in	in	IN
cord-014597-66vd2mdu	2469	23	an	an	DT
cord-014597-66vd2mdu	2469	24	easy	easy	JJ
cord-014597-66vd2mdu	2469	25	way	way	NN
cord-014597-66vd2mdu	2469	26	.	.	.
cord-014597-66vd2mdu	2470	1	After	after	IN
cord-014597-66vd2mdu	2470	2	the	the	DT
cord-014597-66vd2mdu	2470	3	order	order	NN
cord-014597-66vd2mdu	2470	4	process	process	NN
cord-014597-66vd2mdu	2470	5	,	,	,
cord-014597-66vd2mdu	2470	6	test	test	NN
cord-014597-66vd2mdu	2470	7	items	item	NNS
cord-014597-66vd2mdu	2470	8	can	can	MD
cord-014597-66vd2mdu	2470	9	be	be	VB
cord-014597-66vd2mdu	2470	10	shipped	ship	VBN
cord-014597-66vd2mdu	2470	11	to	to	IN
cord-014597-66vd2mdu	2470	12	the	the	DT
cord-014597-66vd2mdu	2470	13	lab	lab	NN
cord-014597-66vd2mdu	2470	14	.	.	.
cord-014597-66vd2mdu	2471	1	Assays	assay	NNS
cord-014597-66vd2mdu	2471	2	will	will	MD
cord-014597-66vd2mdu	2471	3	be	be	VB
cord-014597-66vd2mdu	2471	4	executed	execute	VBN
cord-014597-66vd2mdu	2471	5	on	on	IN
cord-014597-66vd2mdu	2471	6	the	the	DT
cord-014597-66vd2mdu	2471	7	fully	fully	RB
cord-014597-66vd2mdu	2471	8	automated	automate	VBN
cord-014597-66vd2mdu	2471	9	platform	platform	NN
cord-014597-66vd2mdu	2471	10	.	.	.
cord-014597-66vd2mdu	2472	1	By	by	IN
cord-014597-66vd2mdu	2472	2	capturing	capture	VBG
cord-014597-66vd2mdu	2472	3	in	in	IN
cord-014597-66vd2mdu	2472	4	process	process	NN
cord-014597-66vd2mdu	2472	5	data	datum	NNS
cord-014597-66vd2mdu	2472	6	as	as	RB
cord-014597-66vd2mdu	2472	7	well	well	RB
cord-014597-66vd2mdu	2472	8	as	as	IN
cord-014597-66vd2mdu	2472	9	environmental	environmental	JJ
cord-014597-66vd2mdu	2472	10	conditions	condition	NNS
cord-014597-66vd2mdu	2472	11	,	,	,
cord-014597-66vd2mdu	2472	12	a	a	DT
cord-014597-66vd2mdu	2472	13	real	real	JJ
cord-014597-66vd2mdu	2472	14	complete	complete	JJ
cord-014597-66vd2mdu	2472	15	data	data	NN
cord-014597-66vd2mdu	2472	16	set	set	NN
cord-014597-66vd2mdu	2472	17	is	be	VBZ
cord-014597-66vd2mdu	2472	18	leading	lead	VBG
cord-014597-66vd2mdu	2472	19	to	to	IN
cord-014597-66vd2mdu	2472	20	comprehensively	comprehensively	RB
cord-014597-66vd2mdu	2472	21	results	result	NNS
cord-014597-66vd2mdu	2472	22	.	.	.
cord-014597-66vd2mdu	2473	1	As	as	RB
cord-014597-66vd2mdu	2473	2	soon	soon	RB
cord-014597-66vd2mdu	2473	3	as	as	IN
cord-014597-66vd2mdu	2473	4	results	result	NNS
cord-014597-66vd2mdu	2473	5	are	be	VBP
cord-014597-66vd2mdu	2473	6	available	available	JJ
cord-014597-66vd2mdu	2473	7	during	during	IN
cord-014597-66vd2mdu	2473	8	the	the	DT
cord-014597-66vd2mdu	2473	9	process	process	NN
cord-014597-66vd2mdu	2473	10	,	,	,
cord-014597-66vd2mdu	2473	11	the	the	DT
cord-014597-66vd2mdu	2473	12	view	view	NN
cord-014597-66vd2mdu	2473	13	and	and	CC
cord-014597-66vd2mdu	2473	14	analysing	analysing	NN
cord-014597-66vd2mdu	2473	15	can	can	MD
cord-014597-66vd2mdu	2473	16	be	be	VB
cord-014597-66vd2mdu	2473	17	done	do	VBN
cord-014597-66vd2mdu	2473	18	in	in	IN
cord-014597-66vd2mdu	2473	19	a	a	DT
cord-014597-66vd2mdu	2473	20	secure	secure	JJ
cord-014597-66vd2mdu	2473	21	cloud	cloud	NN
cord-014597-66vd2mdu	2473	22	.	.	.
cord-014597-66vd2mdu	2474	1	The	the	DT
cord-014597-66vd2mdu	2474	2	service	service	NN
cord-014597-66vd2mdu	2474	3	can	can	MD
cord-014597-66vd2mdu	2474	4	be	be	VB
cord-014597-66vd2mdu	2474	5	used	use	VBN
cord-014597-66vd2mdu	2474	6	for	for	IN
cord-014597-66vd2mdu	2474	7	single	single	JJ
cord-014597-66vd2mdu	2474	8	experiments	experiment	NNS
cord-014597-66vd2mdu	2474	9	in	in	IN
cord-014597-66vd2mdu	2474	10	low	low	JJ
cord-014597-66vd2mdu	2474	11	throughput	throughput	NN
cord-014597-66vd2mdu	2474	12	applications	application	NNS
cord-014597-66vd2mdu	2474	13	and	and	CC
cord-014597-66vd2mdu	2474	14	is	be	VBZ
cord-014597-66vd2mdu	2474	15	therefore	therefore	RB
cord-014597-66vd2mdu	2474	16	a	a	DT
cord-014597-66vd2mdu	2474	17	benefit	benefit	NN
cord-014597-66vd2mdu	2474	18	for	for	IN
cord-014597-66vd2mdu	2474	19	labs	lab	NNS
cord-014597-66vd2mdu	2474	20	which	which	WDT
cord-014597-66vd2mdu	2474	21	can	can	MD
cord-014597-66vd2mdu	2474	22	not	not	RB
cord-014597-66vd2mdu	2474	23	afford	afford	VB
cord-014597-66vd2mdu	2474	24	automated	automate	VBN
cord-014597-66vd2mdu	2474	25	infrastructure	infrastructure	NN
cord-014597-66vd2mdu	2474	26	or	or	CC
cord-014597-66vd2mdu	2474	27	the	the	DT
cord-014597-66vd2mdu	2474	28	staff	staff	NN
cord-014597-66vd2mdu	2474	29	for	for	IN
cord-014597-66vd2mdu	2474	30	the	the	DT
cord-014597-66vd2mdu	2474	31	maintenance	maintenance	NN
cord-014597-66vd2mdu	2474	32	for	for	IN
cord-014597-66vd2mdu	2474	33	such	such	JJ
cord-014597-66vd2mdu	2474	34	platforms	platform	NNS
cord-014597-66vd2mdu	2474	35	.	.	.
cord-014597-66vd2mdu	2475	1	Extensive	extensive	JJ
cord-014597-66vd2mdu	2475	2	monitoring	monitoring	NN
cord-014597-66vd2mdu	2475	3	and	and	CC
cord-014597-66vd2mdu	2475	4	data	datum	NNS
cord-014597-66vd2mdu	2475	5	capturing	capture	VBG
cord-014597-66vd2mdu	2475	6	during	during	IN
cord-014597-66vd2mdu	2475	7	the	the	DT
cord-014597-66vd2mdu	2475	8	run	run	NN
cord-014597-66vd2mdu	2475	9	leads	lead	VBZ
cord-014597-66vd2mdu	2475	10	to	to	IN
cord-014597-66vd2mdu	2475	11	a	a	DT
cord-014597-66vd2mdu	2475	12	gapless	gapless	JJ
cord-014597-66vd2mdu	2475	13	data	data	NN
cord-014597-66vd2mdu	2475	14	trail	trail	NN
cord-014597-66vd2mdu	2475	15	and	and	CC
cord-014597-66vd2mdu	2475	16	the	the	DT
cord-014597-66vd2mdu	2475	17	possibility	possibility	NN
cord-014597-66vd2mdu	2475	18	of	of	IN
cord-014597-66vd2mdu	2475	19	detailed	detailed	JJ
cord-014597-66vd2mdu	2475	20	result	result	NN
cord-014597-66vd2mdu	2475	21	analysis	analysis	NN
cord-014597-66vd2mdu	2475	22	.	.	.
cord-014597-66vd2mdu	2476	1	Due	due	IN
cord-014597-66vd2mdu	2476	2	to	to	IN
cord-014597-66vd2mdu	2476	3	automated	automate	VBN
cord-014597-66vd2mdu	2476	4	processing	processing	NN
cord-014597-66vd2mdu	2476	5	the	the	DT
cord-014597-66vd2mdu	2476	6	reproducibility	reproducibility	NN
cord-014597-66vd2mdu	2476	7	is	be	VBZ
cord-014597-66vd2mdu	2476	8	increased	increase	VBN
cord-014597-66vd2mdu	2476	9	associated	associate	VBN
cord-014597-66vd2mdu	2476	10	with	with	IN
cord-014597-66vd2mdu	2476	11	direct	direct	JJ
cord-014597-66vd2mdu	2476	12	reduction	reduction	NN
cord-014597-66vd2mdu	2476	13	of	of	IN
cord-014597-66vd2mdu	2476	14	costs	cost	NNS
cord-014597-66vd2mdu	2476	15	and	and	CC
cord-014597-66vd2mdu	2476	16	time	time	NN
cord-014597-66vd2mdu	2476	17	.	.	.
cord-014597-66vd2mdu	2477	1	The	the	DT
cord-014597-66vd2mdu	2477	2	centralized	centralized	JJ
cord-014597-66vd2mdu	2477	3	service	service	NN
cord-014597-66vd2mdu	2477	4	paired	pair	VBN
cord-014597-66vd2mdu	2477	5	with	with	IN
cord-014597-66vd2mdu	2477	6	specific	specific	JJ
cord-014597-66vd2mdu	2477	7	know	know	VB
cord-014597-66vd2mdu	2477	8	-	-	HYPH
cord-014597-66vd2mdu	2477	9	how	how	NN
cord-014597-66vd2mdu	2477	10	allows	allow	VBZ
cord-014597-66vd2mdu	2477	11	up	up	RB
cord-014597-66vd2mdu	2477	12	-	-	HYPH
cord-014597-66vd2mdu	2477	13	scaling	scaling	NN
cord-014597-66vd2mdu	2477	14	of	of	IN
cord-014597-66vd2mdu	2477	15	processes	process	NNS
cord-014597-66vd2mdu	2477	16	at	at	IN
cord-014597-66vd2mdu	2477	17	any	any	DT
cord-014597-66vd2mdu	2477	18	time	time	NN
cord-014597-66vd2mdu	2477	19	.	.	.
cord-014597-66vd2mdu	2478	1	The	the	DT
cord-014597-66vd2mdu	2478	2	web	web	NN
cord-014597-66vd2mdu	2478	3	-	-	HYPH
cord-014597-66vd2mdu	2478	4	based	base	VBN
cord-014597-66vd2mdu	2478	5	interface	interface	NN
cord-014597-66vd2mdu	2478	6	provides	provide	VBZ
cord-014597-66vd2mdu	2478	7	a	a	DT
cord-014597-66vd2mdu	2478	8	flexible	flexible	JJ
cord-014597-66vd2mdu	2478	9	guidance	guidance	NN
cord-014597-66vd2mdu	2478	10	for	for	IN
cord-014597-66vd2mdu	2478	11	the	the	DT
cord-014597-66vd2mdu	2478	12	user	user	NN
cord-014597-66vd2mdu	2478	13	and	and	CC
cord-014597-66vd2mdu	2478	14	the	the	DT
cord-014597-66vd2mdu	2478	15	online	online	JJ
cord-014597-66vd2mdu	2478	16	order	order	NN
cord-014597-66vd2mdu	2478	17	gives	give	VBZ
cord-014597-66vd2mdu	2478	18	24/7	24/7	RP
cord-014597-66vd2mdu	2478	19	access	access	NN
cord-014597-66vd2mdu	2478	20	on	on	IN
cord-014597-66vd2mdu	2478	21	the	the	DT
cord-014597-66vd2mdu	2478	22	infrastructure	infrastructure	NN
cord-014597-66vd2mdu	2478	23	,	,	,
cord-014597-66vd2mdu	2478	24	leading	lead	VBG
cord-014597-66vd2mdu	2478	25	to	to	IN
cord-014597-66vd2mdu	2478	26	a	a	DT
cord-014597-66vd2mdu	2478	27	fast	fast	JJ
cord-014597-66vd2mdu	2478	28	reliable	reliable	JJ
cord-014597-66vd2mdu	2478	29	result	result	NN
cord-014597-66vd2mdu	2478	30	generation	generation	NN
cord-014597-66vd2mdu	2478	31	.	.	.
cord-014597-66vd2mdu	2479	1	Furthermore	furthermore	RB
cord-014597-66vd2mdu	2479	2	the	the	DT
cord-014597-66vd2mdu	2479	3	secure	secure	JJ
cord-014597-66vd2mdu	2479	4	interaction	interaction	NN
cord-014597-66vd2mdu	2479	5	with	with	IN
cord-014597-66vd2mdu	2479	6	additional	additional	JJ
cord-014597-66vd2mdu	2479	7	services	service	NNS
cord-014597-66vd2mdu	2479	8	e.g.	e.g.	IN
cord-014597-66vd2mdu	2479	9	other	other	JJ
cord-014597-66vd2mdu	2479	10	specific	specific	JJ
cord-014597-66vd2mdu	2479	11	data	data	NN
cord-014597-66vd2mdu	2479	12	analysis	analysis	NN
cord-014597-66vd2mdu	2479	13	tool	tool	NN
cord-014597-66vd2mdu	2479	14	is	be	VBZ
cord-014597-66vd2mdu	2479	15	possible	possible	JJ
cord-014597-66vd2mdu	2479	16	.	.	.
cord-014597-66vd2mdu	2480	1	This	this	DT
cord-014597-66vd2mdu	2480	2	dynamic	dynamic	JJ
cord-014597-66vd2mdu	2480	3	access	access	NN
cord-014597-66vd2mdu	2480	4	to	to	IN
cord-014597-66vd2mdu	2480	5	automation	automation	NN
cord-014597-66vd2mdu	2480	6	offers	offer	VBZ
cord-014597-66vd2mdu	2480	7	high	high	JJ
cord-014597-66vd2mdu	2480	8	flexibility	flexibility	NN
cord-014597-66vd2mdu	2480	9	for	for	IN
cord-014597-66vd2mdu	2480	10	low	low	JJ
cord-014597-66vd2mdu	2480	11	throughput	throughput	NN
cord-014597-66vd2mdu	2480	12	experiments	experiment	NNS
cord-014597-66vd2mdu	2480	13	and	and	CC
cord-014597-66vd2mdu	2480	14	will	will	MD
cord-014597-66vd2mdu	2480	15	push	push	VB
cord-014597-66vd2mdu	2480	16	high	high	JJ
cord-014597-66vd2mdu	2480	17	quality	quality	NN
cord-014597-66vd2mdu	2480	18	research	research	NN
cord-014597-66vd2mdu	2480	19	and	and	CC
cord-014597-66vd2mdu	2480	20	drug	drug	NN
cord-014597-66vd2mdu	2480	21	development	development	NN
cord-014597-66vd2mdu	2480	22	in	in	IN
cord-014597-66vd2mdu	2480	23	early	early	JJ
cord-014597-66vd2mdu	2480	24	stage	stage	NN
cord-014597-66vd2mdu	2480	25	.	.	.
cord-014597-66vd2mdu	2481	1	Development	development	NN
cord-014597-66vd2mdu	2481	2	of	of	IN
cord-014597-66vd2mdu	2481	3	alternative	alternative	JJ
cord-014597-66vd2mdu	2481	4	animal	animal	NN
cord-014597-66vd2mdu	2481	5	cell	cell	NN
cord-014597-66vd2mdu	2481	6	technology	technology	NN
cord-014597-66vd2mdu	2481	7	platforms	platform	NNS
cord-014597-66vd2mdu	2481	8	:	:	:
cord-014597-66vd2mdu	2482	1	CHO	CHO	NNP
cord-014597-66vd2mdu	2482	2	based	base	VBN
cord-014597-66vd2mdu	2482	3	cell	cell	NN
cord-014597-66vd2mdu	2482	4	-	-	HYPH
cord-014597-66vd2mdu	2482	5	free	free	JJ
cord-014597-66vd2mdu	2482	6	protein	protein	NN
cord-014597-66vd2mdu	2482	7	synthesis	synthesis	NN
cord-014597-66vd2mdu	2482	8	systems	system	NNS
cord-014597-66vd2mdu	2482	9	for	for	IN
cord-014597-66vd2mdu	2482	10	the	the	DT
cord-014597-66vd2mdu	2482	11	production	production	NN
cord-014597-66vd2mdu	2482	12	of	of	IN
cord-014597-66vd2mdu	2482	13	"	"	``
cord-014597-66vd2mdu	2482	14	difficult	difficult	JJ
cord-014597-66vd2mdu	2482	15	-	-	HYPH
cord-014597-66vd2mdu	2482	16	to	to	TO
cord-014597-66vd2mdu	2482	17	-	-	HYPH
cord-014597-66vd2mdu	2482	18	express	express	VB
cord-014597-66vd2mdu	2482	19	"	"	''
cord-014597-66vd2mdu	2482	20	proteins	protein	NNS
cord-014597-66vd2mdu	2482	21	Lena	Lena	NNP
cord-014597-66vd2mdu	2482	22	Thoring	Thoring	NNP
cord-014597-66vd2mdu	2482	23	1	1	CD
cord-014597-66vd2mdu	2482	24	,	,	,
cord-014597-66vd2mdu	2483	1	2	2	LS
cord-014597-66vd2mdu	2483	2	Background	Background	NNP
cord-014597-66vd2mdu	2483	3	Nowadays	nowadays	RB
cord-014597-66vd2mdu	2483	4	,	,	,
cord-014597-66vd2mdu	2483	5	animal	animal	NN
cord-014597-66vd2mdu	2483	6	cell	cell	NN
cord-014597-66vd2mdu	2483	7	technologies	technology	NNS
cord-014597-66vd2mdu	2483	8	are	be	VBP
cord-014597-66vd2mdu	2483	9	commonly	commonly	RB
cord-014597-66vd2mdu	2483	10	used	use	VBN
cord-014597-66vd2mdu	2483	11	for	for	IN
cord-014597-66vd2mdu	2483	12	a	a	DT
cord-014597-66vd2mdu	2483	13	broad	broad	JJ
cord-014597-66vd2mdu	2483	14	range	range	NN
cord-014597-66vd2mdu	2483	15	of	of	IN
cord-014597-66vd2mdu	2483	16	medical	medical	JJ
cord-014597-66vd2mdu	2483	17	and	and	CC
cord-014597-66vd2mdu	2483	18	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	2483	19	applications	application	NNS
cord-014597-66vd2mdu	2483	20	.	.	.
cord-014597-66vd2mdu	2484	1	One	one	CD
cord-014597-66vd2mdu	2484	2	main	main	JJ
cord-014597-66vd2mdu	2484	3	topic	topic	NN
cord-014597-66vd2mdu	2484	4	of	of	IN
cord-014597-66vd2mdu	2484	5	these	these	DT
cord-014597-66vd2mdu	2484	6	technologies	technology	NNS
cord-014597-66vd2mdu	2484	7	is	be	VBZ
cord-014597-66vd2mdu	2484	8	the	the	DT
cord-014597-66vd2mdu	2484	9	production	production	NN
cord-014597-66vd2mdu	2484	10	of	of	IN
cord-014597-66vd2mdu	2484	11	proteins	protein	NNS
cord-014597-66vd2mdu	2484	12	used	use	VBN
cord-014597-66vd2mdu	2484	13	for	for	IN
cord-014597-66vd2mdu	2484	14	therapeutical	therapeutical	JJ
cord-014597-66vd2mdu	2484	15	purposes	purpose	NNS
cord-014597-66vd2mdu	2484	16	.	.	.
cord-014597-66vd2mdu	2485	1	These	these	DT
cord-014597-66vd2mdu	2485	2	in	in	IN
cord-014597-66vd2mdu	2485	3	vivo	vivo	NN
cord-014597-66vd2mdu	2485	4	production	production	NN
cord-014597-66vd2mdu	2485	5	processes	process	NNS
cord-014597-66vd2mdu	2485	6	are	be	VBP
cord-014597-66vd2mdu	2485	7	often	often	RB
cord-014597-66vd2mdu	2485	8	time	time	NN
cord-014597-66vd2mdu	2485	9	consuming	consume	VBG
cord-014597-66vd2mdu	2485	10	and	and	CC
cord-014597-66vd2mdu	2485	11	limited	limit	VBN
cord-014597-66vd2mdu	2485	12	in	in	IN
cord-014597-66vd2mdu	2485	13	production	production	NN
cord-014597-66vd2mdu	2485	14	of	of	IN
cord-014597-66vd2mdu	2485	15	so	so	RB
cord-014597-66vd2mdu	2485	16	called	call	VBN
cord-014597-66vd2mdu	2485	17	"	"	``
cord-014597-66vd2mdu	2485	18	difficult	difficult	JJ
cord-014597-66vd2mdu	2485	19	-	-	HYPH
cord-014597-66vd2mdu	2485	20	to	to	TO
cord-014597-66vd2mdu	2485	21	-	-	HYPH
cord-014597-66vd2mdu	2485	22	express	express	VB
cord-014597-66vd2mdu	2485	23	"	"	''
cord-014597-66vd2mdu	2485	24	proteins	protein	NNS
cord-014597-66vd2mdu	2485	25	including	include	VBG
cord-014597-66vd2mdu	2485	26	the	the	DT
cord-014597-66vd2mdu	2485	27	pharmaceutical	pharmaceutical	JJ
cord-014597-66vd2mdu	2485	28	relevant	relevant	JJ
cord-014597-66vd2mdu	2485	29	class	class	NN
cord-014597-66vd2mdu	2485	30	of	of	IN
cord-014597-66vd2mdu	2485	31	membrane	membrane	NN
cord-014597-66vd2mdu	2485	32	proteins	protein	NNS
cord-014597-66vd2mdu	2485	33	.	.	.
cord-014597-66vd2mdu	2486	1	To	to	TO
cord-014597-66vd2mdu	2486	2	overcome	overcome	VB
cord-014597-66vd2mdu	2486	3	these	these	DT
cord-014597-66vd2mdu	2486	4	issues	issue	NNS
cord-014597-66vd2mdu	2486	5	,	,	,
cord-014597-66vd2mdu	2486	6	novel	novel	JJ
cord-014597-66vd2mdu	2486	7	cell	cell	NN
cord-014597-66vd2mdu	2486	8	-	-	HYPH
cord-014597-66vd2mdu	2486	9	free	free	JJ
cord-014597-66vd2mdu	2486	10	protein	protein	NN
cord-014597-66vd2mdu	2486	11	synthesis	synthesis	NN
cord-014597-66vd2mdu	2486	12	platforms	platform	NNS
cord-014597-66vd2mdu	2486	13	were	be	VBD
cord-014597-66vd2mdu	2486	14	developed	develop	VBN
cord-014597-66vd2mdu	2486	15	based	base	VBN
cord-014597-66vd2mdu	2486	16	on	on	IN
cord-014597-66vd2mdu	2486	17	the	the	DT
cord-014597-66vd2mdu	2486	18	industrial	industrial	JJ
cord-014597-66vd2mdu	2486	19	working	working	NN
cord-014597-66vd2mdu	2486	20	horse	horse	NN
cord-014597-66vd2mdu	2486	21	CHO	CHO	NNP
cord-014597-66vd2mdu	2486	22	cells	cell	NNS
cord-014597-66vd2mdu	2486	23	[	[	-LRB-
cord-014597-66vd2mdu	2486	24	1	1	CD
cord-014597-66vd2mdu	2486	25	]	]	-RRB-
cord-014597-66vd2mdu	2486	26	.	.	.
cord-014597-66vd2mdu	2487	1	Cell	cell	NN
cord-014597-66vd2mdu	2487	2	lysates	lysate	NNS
cord-014597-66vd2mdu	2487	3	provide	provide	VBP
cord-014597-66vd2mdu	2487	4	a	a	DT
cord-014597-66vd2mdu	2487	5	basis	basis	NN
cord-014597-66vd2mdu	2487	6	for	for	IN
cord-014597-66vd2mdu	2487	7	this	this	DT
cord-014597-66vd2mdu	2487	8	technology	technology	NN
cord-014597-66vd2mdu	2487	9	by	by	IN
cord-014597-66vd2mdu	2487	10	including	include	VBG
cord-014597-66vd2mdu	2487	11	all	all	DT
cord-014597-66vd2mdu	2487	12	components	component	NNS
cord-014597-66vd2mdu	2487	13	of	of	IN
cord-014597-66vd2mdu	2487	14	the	the	DT
cord-014597-66vd2mdu	2487	15	translational	translational	JJ
cord-014597-66vd2mdu	2487	16	machinery	machinery	NN
cord-014597-66vd2mdu	2487	17	and	and	CC
cord-014597-66vd2mdu	2487	18	enabling	enable	VBG
cord-014597-66vd2mdu	2487	19	protein	protein	NN
cord-014597-66vd2mdu	2487	20	production	production	NN
cord-014597-66vd2mdu	2487	21	within	within	IN
cord-014597-66vd2mdu	2487	22	a	a	DT
cord-014597-66vd2mdu	2487	23	few	few	JJ
cord-014597-66vd2mdu	2487	24	hours	hour	NNS
cord-014597-66vd2mdu	2487	25	.	.	.
cord-014597-66vd2mdu	2488	1	Microsomal	Microsomal	NNP
cord-014597-66vd2mdu	2488	2	structures	structure	NNS
cord-014597-66vd2mdu	2488	3	present	present	JJ
cord-014597-66vd2mdu	2488	4	in	in	IN
cord-014597-66vd2mdu	2488	5	CHO	CHO	NNP
cord-014597-66vd2mdu	2488	6	cell	cell	NN
cord-014597-66vd2mdu	2488	7	lysates	lysate	NNS
cord-014597-66vd2mdu	2488	8	enable	enable	VBP
cord-014597-66vd2mdu	2488	9	posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	2488	10	modification	modification	NN
cord-014597-66vd2mdu	2488	11	of	of	IN
cord-014597-66vd2mdu	2488	12	target	target	NN
cord-014597-66vd2mdu	2488	13	proteins	protein	NNS
cord-014597-66vd2mdu	2488	14	and	and	CC
cord-014597-66vd2mdu	2488	15	insertion	insertion	NN
cord-014597-66vd2mdu	2488	16	of	of	IN
cord-014597-66vd2mdu	2488	17	membrane	membrane	NN
cord-014597-66vd2mdu	2488	18	proteins	protein	NNS
cord-014597-66vd2mdu	2488	19	into	into	IN
cord-014597-66vd2mdu	2488	20	lipid	lipid	NN
cord-014597-66vd2mdu	2488	21	bilayer	bilayer	NN
cord-014597-66vd2mdu	2488	22	.	.	.
cord-014597-66vd2mdu	2489	1	In	in	IN
cord-014597-66vd2mdu	2489	2	this	this	DT
cord-014597-66vd2mdu	2489	3	study	study	NN
cord-014597-66vd2mdu	2489	4	a	a	DT
cord-014597-66vd2mdu	2489	5	cell	cell	NN
cord-014597-66vd2mdu	2489	6	-	-	HYPH
cord-014597-66vd2mdu	2489	7	free	free	JJ
cord-014597-66vd2mdu	2489	8	protein	protein	NN
cord-014597-66vd2mdu	2489	9	synthesis	synthesis	NN
cord-014597-66vd2mdu	2489	10	platform	platform	NN
cord-014597-66vd2mdu	2489	11	was	be	VBD
cord-014597-66vd2mdu	2489	12	developed	develop	VBN
cord-014597-66vd2mdu	2489	13	based	base	VBN
cord-014597-66vd2mdu	2489	14	on	on	IN
cord-014597-66vd2mdu	2489	15	a	a	DT
cord-014597-66vd2mdu	2489	16	combination	combination	NN
cord-014597-66vd2mdu	2489	17	of	of	IN
cord-014597-66vd2mdu	2489	18	CHO	CHO	NNP
cord-014597-66vd2mdu	2489	19	cell	cell	NN
cord-014597-66vd2mdu	2489	20	lysates	lysate	NNS
cord-014597-66vd2mdu	2489	21	and	and	CC
cord-014597-66vd2mdu	2489	22	a	a	DT
cord-014597-66vd2mdu	2489	23	continuous	continuous	JJ
cord-014597-66vd2mdu	2489	24	exchange	exchange	NN
cord-014597-66vd2mdu	2489	25	reaction	reaction	NN
cord-014597-66vd2mdu	2489	26	format	format	NN
cord-014597-66vd2mdu	2489	27	.	.	.
cord-014597-66vd2mdu	2490	1	The	the	DT
cord-014597-66vd2mdu	2490	2	continuous	continuous	JJ
cord-014597-66vd2mdu	2490	3	exchange	exchange	NN
cord-014597-66vd2mdu	2490	4	reactor	reactor	NN
cord-014597-66vd2mdu	2490	5	consists	consist	VBZ
cord-014597-66vd2mdu	2490	6	of	of	IN
cord-014597-66vd2mdu	2490	7	a	a	DT
cord-014597-66vd2mdu	2490	8	twochamber	twochamber	NN
cord-014597-66vd2mdu	2490	9	system	system	NN
cord-014597-66vd2mdu	2490	10	,	,	,
cord-014597-66vd2mdu	2490	11	a	a	DT
cord-014597-66vd2mdu	2490	12	reaction	reaction	NN
cord-014597-66vd2mdu	2490	13	and	and	CC
cord-014597-66vd2mdu	2490	14	a	a	DT
cord-014597-66vd2mdu	2490	15	feeding	feeding	NN
cord-014597-66vd2mdu	2490	16	chamber	chamber	NN
cord-014597-66vd2mdu	2490	17	,	,	,
cord-014597-66vd2mdu	2490	18	separated	separate	VBN
cord-014597-66vd2mdu	2490	19	by	by	IN
cord-014597-66vd2mdu	2490	20	a	a	DT
cord-014597-66vd2mdu	2490	21	semipermeable	semipermeable	JJ
cord-014597-66vd2mdu	2490	22	membrane	membrane	NN
cord-014597-66vd2mdu	2490	23	.	.	.
cord-014597-66vd2mdu	2491	1	Due	due	IN
cord-014597-66vd2mdu	2491	2	to	to	IN
cord-014597-66vd2mdu	2491	3	concentration	concentration	NN
cord-014597-66vd2mdu	2491	4	gradients	gradient	NNS
cord-014597-66vd2mdu	2491	5	,	,	,
cord-014597-66vd2mdu	2491	6	energy	energy	NN
cord-014597-66vd2mdu	2491	7	components	component	NNS
cord-014597-66vd2mdu	2491	8	can	can	MD
cord-014597-66vd2mdu	2491	9	diffuse	diffuse	VB
cord-014597-66vd2mdu	2491	10	to	to	IN
cord-014597-66vd2mdu	2491	11	the	the	DT
cord-014597-66vd2mdu	2491	12	reaction	reaction	NN
cord-014597-66vd2mdu	2491	13	chamber	chamber	NN
cord-014597-66vd2mdu	2491	14	,	,	,
cord-014597-66vd2mdu	2491	15	while	while	IN
cord-014597-66vd2mdu	2491	16	inhibitory	inhibitory	JJ
cord-014597-66vd2mdu	2491	17	byproducts	byproduct	NNS
cord-014597-66vd2mdu	2491	18	are	be	VBP
cord-014597-66vd2mdu	2491	19	continuously	continuously	RB
cord-014597-66vd2mdu	2491	20	removed	remove	VBN
cord-014597-66vd2mdu	2491	21	.	.	.
cord-014597-66vd2mdu	2492	1	Different	different	JJ
cord-014597-66vd2mdu	2492	2	classes	class	NNS
cord-014597-66vd2mdu	2492	3	of	of	IN
cord-014597-66vd2mdu	2492	4	proteins	protein	NNS
cord-014597-66vd2mdu	2492	5	were	be	VBD
cord-014597-66vd2mdu	2492	6	selected	select	VBN
cord-014597-66vd2mdu	2492	7	to	to	TO
cord-014597-66vd2mdu	2492	8	evaluate	evaluate	VB
cord-014597-66vd2mdu	2492	9	the	the	DT
cord-014597-66vd2mdu	2492	10	quality	quality	NN
cord-014597-66vd2mdu	2492	11	of	of	IN
cord-014597-66vd2mdu	2492	12	the	the	DT
cord-014597-66vd2mdu	2492	13	CHO	CHO	NNP
cord-014597-66vd2mdu	2492	14	CECF	CECF	NNP
cord-014597-66vd2mdu	2492	15	system	system	NN
cord-014597-66vd2mdu	2492	16	including	include	VBG
cord-014597-66vd2mdu	2492	17	a	a	DT
cord-014597-66vd2mdu	2492	18	transmembrane	transmembrane	NN
cord-014597-66vd2mdu	2492	19	receptor	receptor	NN
cord-014597-66vd2mdu	2492	20	,	,	,
cord-014597-66vd2mdu	2492	21	a	a	DT
cord-014597-66vd2mdu	2492	22	single	single	JJ
cord-014597-66vd2mdu	2492	23	chain	chain	NN
cord-014597-66vd2mdu	2492	24	variable	variable	NN
cord-014597-66vd2mdu	2492	25	fragment	fragment	NN
cord-014597-66vd2mdu	2492	26	and	and	CC
cord-014597-66vd2mdu	2492	27	an	an	DT
cord-014597-66vd2mdu	2492	28	ion	ion	NN
cord-014597-66vd2mdu	2492	29	channel	channel	NN
cord-014597-66vd2mdu	2492	30	.	.	.
cord-014597-66vd2mdu	2493	1	Cell	cell	NN
cord-014597-66vd2mdu	2493	2	-	-	HYPH
cord-014597-66vd2mdu	2493	3	free	free	JJ
cord-014597-66vd2mdu	2493	4	protein	protein	NN
cord-014597-66vd2mdu	2493	5	synthesis	synthesis	NN
cord-014597-66vd2mdu	2493	6	was	be	VBD
cord-014597-66vd2mdu	2493	7	performed	perform	VBN
cord-014597-66vd2mdu	2493	8	in	in	IN
cord-014597-66vd2mdu	2493	9	the	the	DT
cord-014597-66vd2mdu	2493	10	presence	presence	NN
cord-014597-66vd2mdu	2493	11	of	of	IN
cord-014597-66vd2mdu	2493	12	14	14	CD
cord-014597-66vd2mdu	2493	13	C	c	NN
cord-014597-66vd2mdu	2493	14	leucine	leucine	NN
cord-014597-66vd2mdu	2493	15	for	for	IN
cord-014597-66vd2mdu	2493	16	radio	radio	NN
cord-014597-66vd2mdu	2493	17	labeling	labeling	NN
cord-014597-66vd2mdu	2493	18	of	of	IN
cord-014597-66vd2mdu	2493	19	synthesized	synthesize	VBN
cord-014597-66vd2mdu	2493	20	proteins	protein	NNS
cord-014597-66vd2mdu	2493	21	.	.	.
cord-014597-66vd2mdu	2494	1	Protein	protein	NN
cord-014597-66vd2mdu	2494	2	yield	yield	NN
cord-014597-66vd2mdu	2494	3	was	be	VBD
cord-014597-66vd2mdu	2494	4	quantified	quantify	VBN
cord-014597-66vd2mdu	2494	5	by	by	IN
cord-014597-66vd2mdu	2494	6	TCA	TCA	NNP
cord-014597-66vd2mdu	2494	7	precipitation	precipitation	NN
cord-014597-66vd2mdu	2494	8	of	of	IN
cord-014597-66vd2mdu	2494	9	radio	radio	NN
cord-014597-66vd2mdu	2494	10	labeled	label	VBN
cord-014597-66vd2mdu	2494	11	proteins	protein	NNS
cord-014597-66vd2mdu	2494	12	followed	follow	VBN
cord-014597-66vd2mdu	2494	13	by	by	IN
cord-014597-66vd2mdu	2494	14	scintillation	scintillation	NN
cord-014597-66vd2mdu	2494	15	measurement	measurement	NN
cord-014597-66vd2mdu	2494	16	and	and	CC
cord-014597-66vd2mdu	2494	17	molecular	molecular	JJ
cord-014597-66vd2mdu	2494	18	mass	mass	NN
cord-014597-66vd2mdu	2494	19	was	be	VBD
cord-014597-66vd2mdu	2494	20	detected	detect	VBN
cord-014597-66vd2mdu	2494	21	by	by	IN
cord-014597-66vd2mdu	2494	22	autoradiography	autoradiography	NNP
cord-014597-66vd2mdu	2494	23	.	.	.
cord-014597-66vd2mdu	2495	1	Posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	2495	2	modifications	modification	NNS
cord-014597-66vd2mdu	2495	3	and	and	CC
cord-014597-66vd2mdu	2495	4	activities	activity	NNS
cord-014597-66vd2mdu	2495	5	of	of	IN
cord-014597-66vd2mdu	2495	6	proteins	protein	NNS
cord-014597-66vd2mdu	2495	7	were	be	VBD
cord-014597-66vd2mdu	2495	8	estimated	estimate	VBN
cord-014597-66vd2mdu	2495	9	by	by	IN
cord-014597-66vd2mdu	2495	10	kinase	kinase	NN
cord-014597-66vd2mdu	2495	11	assays	assay	NNS
cord-014597-66vd2mdu	2495	12	,	,	,
cord-014597-66vd2mdu	2495	13	ELISA	ELISA	NNP
cord-014597-66vd2mdu	2495	14	,	,	,
cord-014597-66vd2mdu	2495	15	endoglycosidase	endoglycosidase	NN
cord-014597-66vd2mdu	2495	16	treatment	treatment	NN
cord-014597-66vd2mdu	2495	17	and	and	CC
cord-014597-66vd2mdu	2495	18	electrophysiological	electrophysiological	JJ
cord-014597-66vd2mdu	2495	19	measurements	measurement	NNS
cord-014597-66vd2mdu	2495	20	.	.	.
cord-014597-66vd2mdu	2496	1	The	the	DT
cord-014597-66vd2mdu	2496	2	demonstrated	demonstrate	VBN
cord-014597-66vd2mdu	2496	3	results	result	NNS
cord-014597-66vd2mdu	2496	4	showed	show	VBD
cord-014597-66vd2mdu	2496	5	a	a	DT
cord-014597-66vd2mdu	2496	6	protein	protein	NN
cord-014597-66vd2mdu	2496	7	production	production	NN
cord-014597-66vd2mdu	2496	8	of	of	IN
cord-014597-66vd2mdu	2496	9	up	up	IN
cord-014597-66vd2mdu	2496	10	to	to	IN
cord-014597-66vd2mdu	2496	11	around	around	IN
cord-014597-66vd2mdu	2496	12	1	1	CD
cord-014597-66vd2mdu	2496	13	g	g	NN
cord-014597-66vd2mdu	2496	14	/	/	SYM
cord-014597-66vd2mdu	2496	15	l	l	NN
cord-014597-66vd2mdu	2496	16	while	while	IN
cord-014597-66vd2mdu	2496	17	detecting	detect	VBG
cord-014597-66vd2mdu	2496	18	correct	correct	JJ
cord-014597-66vd2mdu	2496	19	molecular	molecular	JJ
cord-014597-66vd2mdu	2496	20	weights	weight	NNS
cord-014597-66vd2mdu	2496	21	by	by	IN
cord-014597-66vd2mdu	2496	22	autoradiography	autoradiography	NNP
cord-014597-66vd2mdu	2496	23	.	.	.
cord-014597-66vd2mdu	2497	1	Analysis	analysis	NN
cord-014597-66vd2mdu	2497	2	of	of	IN
cord-014597-66vd2mdu	2497	3	the	the	DT
cord-014597-66vd2mdu	2497	4	productivity	productivity	NN
cord-014597-66vd2mdu	2497	5	using	use	VBG
cord-014597-66vd2mdu	2497	6	different	different	JJ
cord-014597-66vd2mdu	2497	7	lysate	lysate	NN
cord-014597-66vd2mdu	2497	8	batches	batch	NNS
cord-014597-66vd2mdu	2497	9	by	by	IN
cord-014597-66vd2mdu	2497	10	the	the	DT
cord-014597-66vd2mdu	2497	11	production	production	NN
cord-014597-66vd2mdu	2497	12	of	of	IN
cord-014597-66vd2mdu	2497	13	the	the	DT
cord-014597-66vd2mdu	2497	14	membrane	membrane	NN
cord-014597-66vd2mdu	2497	15	protein	protein	NN
cord-014597-66vd2mdu	2497	16	EGFR	EGFR	NNP
cord-014597-66vd2mdu	2497	17	revealed	reveal	VBD
cord-014597-66vd2mdu	2497	18	only	only	RB
cord-014597-66vd2mdu	2497	19	minimal	minimal	JJ
cord-014597-66vd2mdu	2497	20	batch	batch	NN
cord-014597-66vd2mdu	2497	21	-	-	HYPH
cord-014597-66vd2mdu	2497	22	to	to	IN
cord-014597-66vd2mdu	2497	23	-	-	HYPH
cord-014597-66vd2mdu	2497	24	batch	batch	NN
cord-014597-66vd2mdu	2497	25	variations	variation	NNS
cord-014597-66vd2mdu	2497	26	(	(	-LRB-
cord-014597-66vd2mdu	2497	27	Fig	Fig	NNP
cord-014597-66vd2mdu	2497	28	.	.	.
cord-014597-66vd2mdu	2497	29	1a	1a	CD
cord-014597-66vd2mdu	2497	30	)	)	-RRB-
cord-014597-66vd2mdu	2497	31	.	.	.
cord-014597-66vd2mdu	2498	1	Posttranslational	posttranslational	JJ
cord-014597-66vd2mdu	2498	2	modifications	modification	NNS
cord-014597-66vd2mdu	2498	3	of	of	IN
cord-014597-66vd2mdu	2498	4	proteins	protein	NNS
cord-014597-66vd2mdu	2498	5	,	,	,
cord-014597-66vd2mdu	2498	6	including	include	VBG
cord-014597-66vd2mdu	2498	7	phosphorylation	phosphorylation	NN
cord-014597-66vd2mdu	2498	8	and	and	CC
cord-014597-66vd2mdu	2498	9	glycosylation	glycosylation	NN
cord-014597-66vd2mdu	2498	10	,	,	,
cord-014597-66vd2mdu	2498	11	were	be	VBD
cord-014597-66vd2mdu	2498	12	detected	detect	VBN
cord-014597-66vd2mdu	2498	13	using	use	VBG
cord-014597-66vd2mdu	2498	14	western	western	JJ
cord-014597-66vd2mdu	2498	15	blot	blot	NN
cord-014597-66vd2mdu	2498	16	and	and	CC
cord-014597-66vd2mdu	2498	17	autoradiography	autoradiography	NN
cord-014597-66vd2mdu	2498	18	(	(	-LRB-
cord-014597-66vd2mdu	2498	19	Fig	Fig	NNP
cord-014597-66vd2mdu	2498	20	.	.	.
cord-014597-66vd2mdu	2498	21	1b	1b	CD
cord-014597-66vd2mdu	2498	22	)	)	-RRB-
cord-014597-66vd2mdu	2498	23	.	.	.
cord-014597-66vd2mdu	2499	1	Evaluation	evaluation	NN
cord-014597-66vd2mdu	2499	2	of	of	IN
cord-014597-66vd2mdu	2499	3	localization	localization	NN
cord-014597-66vd2mdu	2499	4	of	of	IN
cord-014597-66vd2mdu	2499	5	membrane	membrane	NN
cord-014597-66vd2mdu	2499	6	embedded	embed	VBN
cord-014597-66vd2mdu	2499	7	eYFP	eYFP	NNP
cord-014597-66vd2mdu	2499	8	fusion	fusion	NN
cord-014597-66vd2mdu	2499	9	proteins	protein	NNS
cord-014597-66vd2mdu	2499	10	by	by	IN
cord-014597-66vd2mdu	2499	11	confocal	confocal	JJ
cord-014597-66vd2mdu	2499	12	laser	laser	NN
cord-014597-66vd2mdu	2499	13	scanning	scanning	NN
cord-014597-66vd2mdu	2499	14	microscopy	microscopy	NNS
cord-014597-66vd2mdu	2499	15	resulted	result	VBN
cord-014597-66vd2mdu	2499	16	in	in	IN
cord-014597-66vd2mdu	2499	17	the	the	DT
cord-014597-66vd2mdu	2499	18	detection	detection	NN
cord-014597-66vd2mdu	2499	19	of	of	IN
cord-014597-66vd2mdu	2499	20	proteins	protein	NNS
cord-014597-66vd2mdu	2499	21	in	in	IN
cord-014597-66vd2mdu	2499	22	the	the	DT
cord-014597-66vd2mdu	2499	23	microsomal	microsomal	NNP
cord-014597-66vd2mdu	2499	24	fraction	fraction	NN
cord-014597-66vd2mdu	2499	25	of	of	IN
cord-014597-66vd2mdu	2499	26	CHO	CHO	NNP
cord-014597-66vd2mdu	2499	27	cell	cell	NN
cord-014597-66vd2mdu	2499	28	lysate	lysate	NN
cord-014597-66vd2mdu	2499	29	.	.	.
cord-014597-66vd2mdu	2500	1	Produced	produce	VBN
cord-014597-66vd2mdu	2500	2	single	single	JJ
cord-014597-66vd2mdu	2500	3	chain	chain	NN
cord-014597-66vd2mdu	2500	4	variable	variable	NN
cord-014597-66vd2mdu	2500	5	fragments	fragment	NNS
cord-014597-66vd2mdu	2500	6	showed	show	VBD
cord-014597-66vd2mdu	2500	7	binding	bind	VBG
cord-014597-66vd2mdu	2500	8	specificity	specificity	NN
cord-014597-66vd2mdu	2500	9	in	in	IN
cord-014597-66vd2mdu	2500	10	ELISA	ELISA	NNP
cord-014597-66vd2mdu	2500	11	experiments	experiment	NNS
cord-014597-66vd2mdu	2500	12	.	.	.
cord-014597-66vd2mdu	2501	1	The	the	DT
cord-014597-66vd2mdu	2501	2	activity	activity	NN
cord-014597-66vd2mdu	2501	3	of	of	IN
cord-014597-66vd2mdu	2501	4	synthesized	synthesize	VBN
cord-014597-66vd2mdu	2501	5	ion	ion	NN
cord-014597-66vd2mdu	2501	6	channels	channel	NNS
cord-014597-66vd2mdu	2501	7	was	be	VBD
cord-014597-66vd2mdu	2501	8	underlined	underline	VBN
cord-014597-66vd2mdu	2501	9	by	by	IN
cord-014597-66vd2mdu	2501	10	electrophysiological	electrophysiological	JJ
cord-014597-66vd2mdu	2501	11	measurements	measurement	NNS
cord-014597-66vd2mdu	2501	12	and	and	CC
cord-014597-66vd2mdu	2501	13	detected	detect	VBD
cord-014597-66vd2mdu	2501	14	single	single	JJ
cord-014597-66vd2mdu	2501	15	channel	channel	NN
cord-014597-66vd2mdu	2501	16	activities	activity	NNS
cord-014597-66vd2mdu	2501	17	.	.	.
cord-014597-66vd2mdu	2502	1	A	a	DT
cord-014597-66vd2mdu	2502	2	cell	cell	NN
cord-014597-66vd2mdu	2502	3	-	-	HYPH
cord-014597-66vd2mdu	2502	4	free	free	JJ
cord-014597-66vd2mdu	2502	5	system	system	NN
cord-014597-66vd2mdu	2502	6	based	base	VBN
cord-014597-66vd2mdu	2502	7	on	on	IN
cord-014597-66vd2mdu	2502	8	CHO	CHO	NNP
cord-014597-66vd2mdu	2502	9	cell	cell	NN
cord-014597-66vd2mdu	2502	10	lysates	lysate	NNS
cord-014597-66vd2mdu	2502	11	for	for	IN
cord-014597-66vd2mdu	2502	12	high	high	JJ
cord-014597-66vd2mdu	2502	13	yield	yield	NN
cord-014597-66vd2mdu	2502	14	production	production	NN
cord-014597-66vd2mdu	2502	15	of	of	IN
cord-014597-66vd2mdu	2502	16	proteins	protein	NNS
cord-014597-66vd2mdu	2502	17	was	be	VBD
cord-014597-66vd2mdu	2502	18	developed	develop	VBN
cord-014597-66vd2mdu	2502	19	that	that	WDT
cord-014597-66vd2mdu	2502	20	provides	provide	VBZ
cord-014597-66vd2mdu	2502	21	a	a	DT
cord-014597-66vd2mdu	2502	22	platform	platform	NN
cord-014597-66vd2mdu	2502	23	for	for	IN
cord-014597-66vd2mdu	2502	24	efficient	efficient	JJ
cord-014597-66vd2mdu	2502	25	production	production	NN
cord-014597-66vd2mdu	2502	26	of	of	IN
cord-014597-66vd2mdu	2502	27	"	"	``
cord-014597-66vd2mdu	2502	28	difficult	difficult	JJ
cord-014597-66vd2mdu	2502	29	-	-	HYPH
cord-014597-66vd2mdu	2502	30	to	to	TO
cord-014597-66vd2mdu	2502	31	-	-	HYPH
cord-014597-66vd2mdu	2502	32	express	express	VB
cord-014597-66vd2mdu	2502	33	"	"	''
cord-014597-66vd2mdu	2502	34	proteins	protein	NNS
cord-014597-66vd2mdu	2502	35	.	.	.
cord-014597-66vd2mdu	2503	1	The	the	DT
cord-014597-66vd2mdu	2503	2	combination	combination	NN
cord-014597-66vd2mdu	2503	3	of	of	IN
cord-014597-66vd2mdu	2503	4	a	a	DT
cord-014597-66vd2mdu	2503	5	CHO	CHO	NNP
cord-014597-66vd2mdu	2503	6	lysate	lysate	NN
cord-014597-66vd2mdu	2503	7	based	base	VBN
cord-014597-66vd2mdu	2503	8	cell	cell	NN
cord-014597-66vd2mdu	2503	9	-	-	HYPH
cord-014597-66vd2mdu	2503	10	free	free	JJ
cord-014597-66vd2mdu	2503	11	system	system	NN
cord-014597-66vd2mdu	2503	12	and	and	CC
cord-014597-66vd2mdu	2503	13	a	a	DT
cord-014597-66vd2mdu	2503	14	continuous	continuous	JJ
cord-014597-66vd2mdu	2503	15	exchange	exchange	NN
cord-014597-66vd2mdu	2503	16	cell	cell	NN
cord-014597-66vd2mdu	2503	17	-	-	HYPH
cord-014597-66vd2mdu	2503	18	free	free	JJ
cord-014597-66vd2mdu	2503	19	system	system	NN
cord-014597-66vd2mdu	2503	20	leads	lead	VBZ
cord-014597-66vd2mdu	2503	21	to	to	TO
cord-014597-66vd2mdu	2503	22	be	be	VB
cord-014597-66vd2mdu	2503	23	a	a	DT
cord-014597-66vd2mdu	2503	24	highly	highly	RB
cord-014597-66vd2mdu	2503	25	efficient	efficient	JJ
cord-014597-66vd2mdu	2503	26	production	production	NN
cord-014597-66vd2mdu	2503	27	system	system	NN
cord-014597-66vd2mdu	2503	28	for	for	IN
cord-014597-66vd2mdu	2503	29	various	various	JJ
cord-014597-66vd2mdu	2503	30	classes	class	NNS
cord-014597-66vd2mdu	2503	31	of	of	IN
cord-014597-66vd2mdu	2503	32	"	"	``
cord-014597-66vd2mdu	2503	33	difficult	difficult	JJ
cord-014597-66vd2mdu	2503	34	-	-	HYPH
cord-014597-66vd2mdu	2503	35	to	to	TO
cord-014597-66vd2mdu	2503	36	-	-	HYPH
cord-014597-66vd2mdu	2503	37	express	express	VB
cord-014597-66vd2mdu	2503	38	"	"	''
cord-014597-66vd2mdu	2503	39	proteins	protein	NNS
cord-014597-66vd2mdu	2503	40	.	.	.
cord-014597-66vd2mdu	2504	1	This	this	DT
cord-014597-66vd2mdu	2504	2	approach	approach	NN
cord-014597-66vd2mdu	2504	3	opens	open	VBZ
cord-014597-66vd2mdu	2504	4	up	up	RP
cord-014597-66vd2mdu	2504	5	a	a	DT
cord-014597-66vd2mdu	2504	6	fast	fast	JJ
cord-014597-66vd2mdu	2504	7	and	and	CC
cord-014597-66vd2mdu	2504	8	cost	cost	NN
cord-014597-66vd2mdu	2504	9	-	-	HYPH
cord-014597-66vd2mdu	2504	10	effective	effective	JJ
cord-014597-66vd2mdu	2504	11	process	process	NN
cord-014597-66vd2mdu	2504	12	pipeline	pipeline	NN
cord-014597-66vd2mdu	2504	13	for	for	IN
cord-014597-66vd2mdu	2504	14	the	the	DT
cord-014597-66vd2mdu	2504	15	production	production	NN
cord-014597-66vd2mdu	2504	16	of	of	IN
cord-014597-66vd2mdu	2504	17	"	"	``
cord-014597-66vd2mdu	2504	18	difficult	difficult	JJ
cord-014597-66vd2mdu	2504	19	-	-	HYPH
cord-014597-66vd2mdu	2504	20	to	to	TO
cord-014597-66vd2mdu	2504	21	-	-	HYPH
cord-014597-66vd2mdu	2504	22	express	express	VB
cord-014597-66vd2mdu	2504	23	"	"	''
cord-014597-66vd2mdu	2504	24	proteins	protein	NNS
cord-014597-66vd2mdu	2504	25	and	and	CC
cord-014597-66vd2mdu	2504	26	shows	show	VBZ
cord-014597-66vd2mdu	2504	27	a	a	DT
cord-014597-66vd2mdu	2504	28	high	high	JJ
cord-014597-66vd2mdu	2504	29	potential	potential	NN
cord-014597-66vd2mdu	2504	30	for	for	IN
cord-014597-66vd2mdu	2504	31	industrial	industrial	JJ
cord-014597-66vd2mdu	2504	32	applications	application	NNS
cord-014597-66vd2mdu	2504	33	including	include	VBG
cord-014597-66vd2mdu	2504	34	screening	screening	NN
cord-014597-66vd2mdu	2504	35	technologies	technology	NNS
cord-014597-66vd2mdu	2504	36	,	,	,
cord-014597-66vd2mdu	2504	37	protein	protein	NN
cord-014597-66vd2mdu	2504	38	structure	structure	NN
cord-014597-66vd2mdu	2504	39	determination	determination	NN
cord-014597-66vd2mdu	2504	40	and	and	CC
cord-014597-66vd2mdu	2504	41	just	just	RB
cord-014597-66vd2mdu	2504	42	-	-	HYPH
cord-014597-66vd2mdu	2504	43	in	in	IN
cord-014597-66vd2mdu	2504	44	-	-	HYPH
cord-014597-66vd2mdu	2504	45	time	time	NN
cord-014597-66vd2mdu	2504	46	protein	protein	NN
cord-014597-66vd2mdu	2504	47	production	production	NN
cord-014597-66vd2mdu	2504	48	processes	process	NNS
cord-014597-66vd2mdu	2504	49	.	.	.