id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310110-haukpwtf	Guo, Jinlei	Limited effect of recombinant human mannose-binding lectin on the infection of novel influenza A (H7N9) virus in vitro	2015-02-27		.txt	text/plain	3447	185	53	title: Limited effect of recombinant human mannose-binding lectin on the infection of novel influenza A (H7N9) virus in vitro Our findings suggest that MBL, the host innate molecule, has differential interference effects with human and avian influenza virus and limited antiviral effect against H7N9 virus. Different concentrations of rhMBL were diluted in HBSS containing Ca 2þ and mixed with influenza virus in a total volume of 100 mL and preincubated at 37 C for 1 h, and then transferred to wells precoated with fetuin (Sigma, USA) and incubated at 37 C for 4 h, After washing, 100 mL of HRP-labeled peanut lectin (3 mg/mL) was added and after 1 h at room temperature, the wells were washed and o-phenylenediamine dihydrochloride in citrate buffer was added, reaction was stopped by 2 M H 2 SO 4 , and the OD at 492 nm was measured. Therefore, the strong MBL-H7N9 virus interaction whereas limited effects on viral HA-receptor binding or NA-mediated releasing, might amplify immune dysfunctions in vivo and confer clinical severity of H7N9 infection via activating complement pathway and further investigates are needed. Human mannan-binding lectin inhibits the infection of influenza A virus without complement	./cache/cord-310110-haukpwtf.txt	./txt/cord-310110-haukpwtf.txt