id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-265658-wjqezs0v	Carranza-Rosales, Pilar	Modeling tuberculosis pathogenesis through ex vivo lung tissue infection	2017-09-12		.txt	text/plain	3239	162	45	Several in vitro and in vivo experimental models have been used to study TB pathogenesis and induction of immune response during Mycobacterium tuberculosis infection. Precision cut lung tissue slices (PCLTS) is an experimental model, in which all the usual cell types of the organ are found, the tissue architecture and the interactions amongst the different cells are maintained. PCLTS in good physiological conditions, monitored by MTT assay and histology, were infected with either virulent Mycobacterium tuberculosis strain H37Rv or the TB vaccine strain Mycobacterium bovis BCG. Lung tissue slices have been used for toxicity studies [3] , biotransformation [4] , metabolism of xenobiotics [5] , and to study infectious agents like coronavirus [6] , retrovirus [7] , influenza and parainfluenza viral strains [2,8e12] , viruses of the bovine respiratory disease complex [13, 14] , respiratory syncytial virus (RSV), and only one bacteria Chlamydophila pneumonia [15] .	./cache/cord-265658-wjqezs0v.txt	./txt/cord-265658-wjqezs0v.txt