id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310535-ay2cdf2w	Cho, Chang-Won	Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages	2017-06-20		.txt	text/plain	4636	262	45	title: Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages As polysaccharides may also have anti-inflammatory functions, we investigated the anti-inflammatory effects and related molecular mechanisms of a crude polysaccharide (HMFO) obtained from HMF of CW in mice with dextran sulfate sodium(DSS-) induced colitis and in lipopolysaccharide-induced RAW 264.7 macrophages. In addition, HMFO inhibited iNOS and COX-2 protein expression, as well as phosphorylated NF-κB p65 levels in the colon tissue of mice with DSS-induced colitis. In macrophages, HMFO inhibited several cytokines and enzymes involved in inflammation such as prostaglandin E(2), nitric oxide, tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2 by attenuating nuclear factor-κB (NF-κB) and mitogen-activated protein kinases. The effects of HMFO on the expression of inflammatory proteins such as COX-2 and iNOS in cytosolic extracts from the colon of colitis mice were detected by Western blot analysis.	./cache/cord-310535-ay2cdf2w.txt	./txt/cord-310535-ay2cdf2w.txt