id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300912-w85t4zz6	Wu, Beibei	n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils	2017-10-25		.txt	text/plain	3699	224	50	title: n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils Our previous study investigated the effect of n-butanol extract from Folium isatidis on lipopolysaccharide (LPS)-induced septic shock. The addition of n-butanol extract from Folium isatidis inhibited this LPS-induced downregulation of CXCR1, CXCR2 and CD62L, and decreased the expression of IL-8 on neutrophils. The isolated neutrophils were treated with either vehicle or increasing concentrations (10, 100 and 1,000 ng/ml) of LPS for 4 h, and the expression levels of CXCR1, CXCR2, TLR2, TLR4, CD11b and CD62L were measured using flow cytometry. n-butanol extract from Folium isatidis prevents the LPS-induced downregulation of CXCR1, CXCR2 and CD62L. Subsequent analysis of the cells using flow cytometry indicated that the extract inhibited the LPS-induced downregulation of CXCR1, CXCR2 and CD62L ( Fig. 2A-C) in a dose-dependent manner. Effect of n-butanol extract from Folium isatidis on the gene expression levels of CXCR1, CXCR2 and CD62L.	./cache/cord-300912-w85t4zz6.txt	./txt/cord-300912-w85t4zz6.txt