id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-258057-ti0rpt0q	Zhao, Kai	Establishment of a Porcine Parvovirus (PPV) LAMP Visual Rapid Detection Method	2020-07-01		.txt	text/plain	3731	223	59	A loop-mediated isothermal amplification (LAMP) assay was established to detect PPV infection. The optimized LAMP program was as follows: 50 min at 59 °C followed by 3 min at 80 °C.The amplified products were analyzed both by visual inspection after staining with SYBR Green I dye and by conventional agarose gel electrophoresis. To verify the specificity of PPV detection by LAMP, the DNA (PPV, PCV2, PRV) and cDNA samples (PRRSV, CSFV) were amplified as sample templates by the LAMP reaction at 59.0℃ for 50 min and terminated at 80℃ for 3 min, respectively. The LAMP detection limit for PPV based on visual observation by addition of J o u r n a l P r e -p r o o f SYBR Green I and by gel electrophoresis analysis was 10 1 copies. Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification Rapid and sensitive diagnosis of porcine parvovirus by loop-mediated isothermal amplification (LAMP) method	./cache/cord-258057-ti0rpt0q.txt	./txt/cord-258057-ti0rpt0q.txt