id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003342-wmmbkmrg	Wang, De-Guo	Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification	2015-04-07		.txt	text/plain	2869	129	42	In this study, a set of LAMP primers were designed targeting the prfA gene sequence of Listeria monocytogenes, and dimethyl sulfoxide (DMSO) as well as Touchdown LAMP were employed to increase the sensitivity and specificity of the LAMP reactions. The results indicate that the detection limit of this novel LAMP assay with the newly designed primers and additives was 10 fg per reaction, which is ten-fold more sensitive than a commercial Isothermal Amplification Kit and hundred-fold more sensitive than previously reported LAMP assays. This highly sensitive LAMP assay has been shown to detect 11 strains of Listeria monocytogenes, and does not detect other Listeria species (including Listeria innocua and Listeria invanovii), providing some advantages in specificity over commercial Isothermal Amplification Kits and previously reported LAMP assay. Loop-mediated isothermal amplification, developed and reported by Notomi et al., in 2000 [1] , can specifically, sensitively and rapidly amplify nucleic acids with two pairs of primers recognizing 6 independent sequences of a target gene under isothermal conditions.	./cache/cord-003342-wmmbkmrg.txt	./txt/cord-003342-wmmbkmrg.txt