key: cord-302433-5cch8w1m authors: Soma, Takehisa; Nakagomi, Osamu; Nakagomi, Toyoko; Mochizuki, Masami title: Detection of Norovirus and Sapovirus from diarrheic dogs and cats in Japan date: 2015-03-26 journal: Microbiol Immunol DOI: 10.1111/1348-0421.12223 sha: doc_id: 302433 cord_uid: 5cch8w1m Norovirus (NoV) and sapovirus (SaV) are important causes of human diarrhea. In this study, between 2007 and 2014 fecal samples were collected from 97 dogs and 83 cats with diarrhea and examined to determine the prevalence of NoV and SaV infections in Japan. To detect caliciviruses, approximately 300 bases targeting the polymerase gene were amplified using RT‐PCR and subjected to phylogenetic and homology analyses. Specific PCR products were obtained from four canine and nine feline samples: two canine and one feline isolate were classified as NoV, two canine isolates as SaV and the remaining eight feline isolates as vesivirus (VeV). The three NoV isolates were classified into the same clade as that of known canine and feline NoVs; their homologies (75.9–92.3%) were higher than those with human genogroup IV (GIV) NoVs (59.1–65.9%). The homology of the feline NoV isolate with previously reported feline NoV isolates was particularly high (91.7–92.3%). Regarding SaV, the two canine isolates were classified into the same clade as known canine SaVs and their homologies (72.5–86.5%) were higher than those with other mammal SaVs (20.7–58.0%). The eight feline VeV isolates were assumed to be feline calicivirus. The present study is the first report of the presence of NoV‐ and SaV‐infected dogs and cats in Japan. The findings suggest there are species‐specific circulations of NoV and SaV among dogs and cats, in Japan. NoV, SaV, VeV, lagovirus and nebovirus, belong to the family Caliciviridae, which comprises small non-enveloped viruses containing approximately 7.7 kb singlestranded RNA; both NoV and SaV are clinically important enteric pathogens in humans (1, 2) . Although canine caliciviruses belonging to VeV have been detected and are widely prevalent in dogs in Japan and Korea, they have not yet been established as an enteric pathogen for dogs (3) (4) (5) (6) . On the other hand, FCV, which is a VeV, is a well-known respiratory pathogen in cats (7) . NoV is classified into at least five genogroups (GI, GII, GIII, GIV and GV) based on their VP1 sequences (8) . Additional genogroups (GVI and GVII) have recently been proposed (9) (10) (11) . GI, GII, GIV and GVI are known to infect humans; of these, GII is the most prevalent (8, 9, 11, 12) . GIV and GVI NoVs have been detected in dogs, whereas GIV NoV has been detected in a cat and a lion (13, 14) . The major HuNoV genogroup GII was also detected recently in the feces of three dogs (15) and the roles of canine and feline NoVs in public health and their clinical impact have been inferentially discussed (16, 17) . SaV is classified into 14 genogroups (GI-GV) based on VP1 sequences (18) . SaV has not yet been detected in cats. Because SaV has been detected in only a few dogs, the relationship between SaV of canine and human origin has not yet been elucidated in detail; however, the zoonotic potential of SaV of dog origins has been preliminarily discussed (16) . Because of their close relationship with humans as companion animals, dogs and cats have attracted special attention with regard to zoonosis; thus, investigation of their epidemiology is meaningful. In this study, we therefore examined the feces of clinical cases for caliciviruses, compared them with known isolates and discuss their diarrheagenic potential. From September 2007 to March 2014, the feces of 97 household dogs and 83 cats with diarrhea were submitted by veterinary clinics throughout Japan to a commercial veterinary laboratory (Marupi Lifetech, Osaka, Japan) for detection of parvovirus. The fecal samples were suspended in sterile saline, the suspensions centrifuged and nucleic acids isolated from the supernatants using a QIAamp DNA Mini Kit (Qiagen, Germantown, MD, USA) according to the manufacture's instructions, after which they were preserved in a freezer. Although this kit is designed for DNA purification, RNA can also be extracted and purified, according to the attached instruction and a previous study (19) . Using a Qiagen One Step RT-PCR kit, caliciviruses were detected by RT-PCR with the p290d-p289d primer pair that has been reported to bind to the polymerase gene of the calicivirus (14, 20) . This primer pair is expected to amplify a 331 bp fragment of SaV and VeV and a 319 bp fragment of NoV. Two point five mL of the extracted RNA was added to 25 mL reaction mixtures containing 5 mL of 5 Â buffer (finally 1.5 mM MgCl 2 ), 0.4 mM of deoxynucleoside triphosphate, 10 U RNase inhibitor (Promega, Madison, WI, USA), 0.8 mM of the primers and 1.0 mL of enzyme mix. The RNA was reverse transcribed at 50°C for 30 min, followed by inactivation of reverse transcriptase and denaturation of cDNA template at 95°C for 15 min. The cDNA was amplified in 40 sequential cycles of denaturation at 94°C for 1 min, annealing at 49°C for 1 min and extension at 72°C for 1 min, followed by a final extension at 72°C for 7 min. The PCR products were electrophoresed at 100 V for 35 min in a 2% agarose gel, after which they were stained with ethidium bromide and visualized under UV illumination. The PCR amplicons were purified using ExoSAP-IT, and submitted to direct sequencing of both strands by a dye-terminator cycle sequencing methodology (Bio Matrix Research, Chiba, Japan). The sequences were aligned with reference strains in the GenBank Database using Clustal W and MEGA 6 software (21) . Phylogenetic trees were constructed employing the neighborjoining method (22) , and their homologies were using BLAST search. To investigate other diarrheagenic viruses in the canine samples, CPV-2 (23), CCoV-I and -II (24), CDV (25), CAdV-1 (26) and RV (27) were examined by PCR. For the feline samples, FPLV (23), CPV-2, FCoV-I and -II (24) and RV were examined. The PCR products (319 or 331 bp) amplified with the p290d-p289d primer pair were detected in four and nine of 97 canine and 83 feline diarrheal samples, respectively. When the nucleic acid sequences of the PCR products of these 13 samples were subjected to phylogenetic analysis, two canine and one feline sample were classified into the clade of NoV, two canine samples were classified into the clade of SaV, and the other eight feline samples were classified into the clade of VeV, as shown in Figure 1 . The partial RdRp sequences of each viral genus were compared with the reference strains in the GenBank database. As shown in Figure 1 , the dog-derived NoV isolates (Ca/Osaka72/2014/JP and Ca/Aichi595/2013/ JP) and cat-derived NoV isolate (Fe/Saitama382/2013/ JP) were classified into the same clade as known CaNoV and FeNoV, showing 75.9-92.3% homologies. This homology rate is higher than that (59.1-65.9%) with GIV HuNoV, which clusters in a different clade on the phylogenetic tree. In addition, the cat-derived isolate (Fe/Saitama382/2013/JP) had a very high homology (91.7-92.3%) with known FeNoV strains (CU081210E and Pistoia1387). In contrast, the homologies of the canine isolates in the present study with each previous CaNoV isolate in the same clade were 77.3-91.8%, whereas those with Ca/HKU_Ca035F and Ca/HKU_ Ca026F not classified into the same clade had low homologies (53.6-57.9%). Two isolates from dogs classified as SaV (Ca/Kyoto619/ 2011/JP and Ca/Mie565/2011/JP) clustered into the same clade in the phylogenetic tree as previously isolated CaSaVs in CA, USA (Ca/AN196 and Ca/AN210D) (Fig. 1) . The homologies among these four SaV strains of dog origin were 72.5-86.5%, which are higher than those with SaV isolates from other mammals including humans (20.7-58.0%). VeVs from the other eight cats were classified as FCV species on the basis of the clustering pattern in the phylogenetic tree (Fig. 1) . Table 2 ). Both NoV and SaV, two isolates of each, were detected in the feces of dogs and one isolate of NoV was detected in the feces of a cat. To the best of our knowledge, these are the first such findings in Japan and only a few studies have detected CaSaV and FeNoV in other parts of the world (14, 28) . In the present study, GIV and GVI NoVs derived from canines and felines could not be discriminated by phylogenetic analysis, presumably because only a short part of the polymerase gene was analyzed; however, three NoV isolates detected in dogs and a cat (Ca/Osaka72/ 2014/JP, Ca/Aichi595/2013/JP and Fe/Saitama382/2013/ JP) were classified into the same clade with known CaNoVs and FeNoVs in the phylogenetic tree. In addition, excluding the strains isolated from Chinese dogs (Ca/HKU_Ca035F and Ca/HKU_Ca026F) whose genogroups have not yet been identified (29) , the homologies of these three isolates with known CaNoV and FeNoV (75.9-92.3%) were higher than those with GIV HuNoV (59.1-65.9%), indicating that the present three isolates form a clade with known CaNoVs and FeNoVs that differs from that of HuNoVs in the phylogenetic tree. It has been suggested that NoV has been circulating among dogs (30) ; the findings of the present study support this, as described above. However, the homologies of the two NoV isolates with known CaNoVs ranged from 77.3 to 91.8% even within the same clade, suggesting the presence of several lineages within the CaNoV genogroups, as described previously (9, 10) . Although only a small number of FeNoV strains have been characterized, the homologies observed between the present feline NoV isolate (Fe/Saitama382/2013/JP) and previous cat-and lion-derived isolates (CU081210E and Pistoia1387) were extremely high (91.7-92.3%), suggesting the presence of a unique circulation of NoV in the Felidae that differs from that in dogs. On the other hand, the SaV isolates from dogs (Ca/ Kyoto619/2011/JP and Ca/Mie565/2011/JP) were classified into the same clade as two canine strains previously reported in the USA and the homologies among these (72.5-86.5%) were markedly higher than those in other mammals (20.7-58.0%), suggesting the presence of a unique circulation of SaV in dogs or the Canidae. CaNoV, FeNoV and CaSaV may have an infection cycle in each animal species or family. However, the potential for human infection by some types of NoV and SaV in dogs and cats cannot be ruled out, because of the following observations: (i) GII NoV, which is the most prevalent human NoV, has been detected from dogs (15); (ii) GIV and GVI NoVs, which are the main genogroups in dogs and cats, have been detected in humans (9, 11) ; and (iii) antibodies against GVI NoV have been detected in human sera (17) . Although a significantly high detection rate of CaNoV in dogs with diarrhea has previously been reported (11) , epidemiologic data have been insufficient and the pathogenic potential of NoV and SaV for dogs and cats remains undetermined. In the present study, we were unable to clarify the pathogenicity of NoVs and SaVs in dogs and cats, because mixed infections with CPV-2 or FPLV, which are the most virulent causes of diarrhea in dogs and cats, were noted in all five samples in which NoV or SaV was detected. In previous studies, most dogs infected with CaNoV were approximately 2-3 months old (13, 30) . A high CaNoV prevalence has been reported in kennels (31) . All four dogs in which NoV and SaV were detected in the present study were also 2 months old and purebred, suggesting that CaNoV and CaSaV infections likely also occur in environments where puppies and kittens are ordinarily kept, such as pet shops and breeding colonies. FCV was detected in 9.6% of fecal specimens from cats in the present study. Although FCV is a representative pathogen for upper respiratory tract, it has been demonstrated not only in laryngopharyngeal secretions but also in feces (32) . Although FCV, which replicates in the upper respiratory epithelial cells, potentially passes through the alimentary tract and is excreted in the feces, a difference in resistance to bile salts has been observed between FCV isolates from respiratory secretions and feces, suggesting the presence of enterocyte-infecting FCV (32) . No other diarrheagenic viruses were detected in one FCV-positive sample (Fe/Tokyo671/2013/JP), and only FCoV-I, which generally causes no or mild symptoms of intestinal infections when it infects alone, was detected in three samples (Fe/Yamaguchi165/2011/ JP, Fe/Saitama1650/2013/JP and Fe/Osaka210/2013/JP). These findings suggest that some type(s) of FCV may be enteric pathogens in cats, but this remains to be elucidated in future studies, including experimental infections. 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