key: cord-331827-amg309uz authors: Keske, Şiran; Gümüş, Terman; Köymen, Tamer; Sandıkçı, Sunay; Tabak, Levet; Ergönül, Önder title: Human metapneumovirus infection: Diagnostic impact of radiologic imaging date: 2019-02-01 journal: J Med Virol DOI: 10.1002/jmv.25402 sha: doc_id: 331827 cord_uid: amg309uz BACKGROUND: Human metapneumovirus (hMPV) is a recently detected virus, which can cause mild to severe respiratory tract infections. Through this study, we aimed to detail the outcomes of hMPV infections. MATERIALS/METHODS: Between January 2012 and November 2017, patients who had hMPV detected in nasopharyngeal or bronchoalveolar lavage by molecular respiratory pathogen tests were evaluated. The Food and Drug Administration cleared multiplexed‐polymerase chain reaction system (Idaho Technology, Salt Lake City, UT) was used for diagnosis. Chest radiography (CR) and computed tomography (CT) were evaluated by an expert radiologist. RESULTS: In total 100 patients were included, the mean age was 22.9 (0‐87) years, and 50% were male. The hospitalization rate was 52%. Lower respiratory system infection (LRTI) was diagnosed in 44 patients with clinical findings, and in 31 patients out of 44 the radiological findings supported the diagnosis. The LRTI rate was significantly higher in adults than children (66.7%‐32.8%; P = 0.001). In CR, peribronchovascular infiltration (PI) was the most common feature seen in 14 out of 18 patients and was generally bilateral (13 out of 18 patients). In CT imaging, ground‐glass opacity was the most common finding seen in 11 out of 16 patients and nodular consolidation in five patients. Ribavirin was given to four patients, three of whom were severe and required respiratory support. None of the patients died of hMPV infection. CONCLUSIONS: The ground‐glass opacity in CT was similar to other respiratory virus infections, and PI in CR was very common and typical; however, nodular consolidation that may mimic bacterial infection was seen in one‐fourth of CT. Human metapneumovirus (hMPV) was defined in 2001. It causes mild to severe respiratory system infections. [1] [2] [3] Due to the increasing availability of molecular based tests, reporting of hMPV infection is increasing all over the world. [4] [5] [6] The hMPV has been reported to be one of the most commonly detected viruses that cause influenza-like illness 7 and community-acquired pneumonia 6 in both adults and children. Radiologic findings of hMPV infection have not been reported to be different from other viral pathogens in studies with high sample sizes; 8 however, it has been reported that studies including radiologic findings of hMPV infections were commonly done among immunocompromised hosts. 9 hMPV infection may cause fatality in patients with hematologic malignancy. 3, 10 The infection presents with a wide variation of clinical conditions from asymptomatic to fatal. We aimed to describe the clinical course of the disease by detailing the clinical and radiological findings and the outcome. We included patients from inpatient and outpatient departments who had hMPV in nasopharyngeal or bronchoalveolar lavage, diagnosed using the molecular respiratory pathogen test. The study was conducted in a 265-bed private hospital in Istanbul between January 2012 and November 2017. This was a retrospective study performed by reviewing the patient charts. The clinical features of the patients, demographic characteristics, chronic diseases including malignant disorders, diabetes mellitus, chronic kidney and liver diseases, and detailed information about antibiotic and antiviral drugs were collected by chart review. Complete blood count, C-reactive protein, and liver and kidney function tests were studied among all patients. Bacterial cultures (throat, sputum, blood, and urine) were obtained, and procalcitonin (PCT) was studied in patients with suspicion of bacterial infection and/or in critical patients. Chlamydia pneumoniae and Mycoplasma pneumoniae were included in multiplex polymerase chain reaction (PCR) tests, and urine Legionella antigen test was performed in case of suspicion. The Food and Drug Administration cleared multiplexed-respiratory PCR system BioFireFilmArray (Idaho Technology), which detects 17 viral pathogens including hMPV, and three bacterial species were used for the molecular detection of hMPV. Chest radiography (CR) and computed tomography (CT) were done if lower respiratory system infection (LRSI) was suspected. The radiological imaging of patients was evaluated by a single dedicated radiologist. The upper respiratory system infection (URSI) was defined based on the modified CENTOR score that was developed for acute tonsillitis/ pharyngitis 11 and deduced from the recommendation of the Centers for Disease Control and Prevention and Infectious Diseases Society of America. 12, 13 The LRSI is defined as infectious inflammation of the lower respiratory tract and refers to acute pneumonia, acute bronchitis, and acute bronchiolitis. Clinical LRSI is a syndrome characterized by symptoms consistent with respiratory tract infection (such as fever, cough, sputum, and dyspnea) and lung auscultation findings (crackles, rhonchus, and decreased lung sounds). 14 Radiological LRSI is defined as radiological findings of the lung, including consolidation, cavitation, peribronchial ground-glass opacity (GGO), airspace consolidation, and small nodules. In the statistical analysis, the t test for continuous variables and the χ 2 test for the comparison of categorical variables were used. In the analysis, STATA 11 (StataCorp, College Station, TX) was used, and P < 0.05 was set as significant. The Institutional Review Board of Koç University approved the study. Our study included 100 patients ( Figure 1 ). The mean age of the patients was 22.9 (0-87) years, and 50% of them were male (Table 1) . Two-third (67%) of the patients were under 18 years old. In 14 patients, more than one virus was detected. Concomitant agents were as follows: seven rhino/enterovirus, five influenza, two coronavirus, and one respiratory syncytial virus. The cases were most commonly seen between November and June, and 21% of the cases were seen in December ( The antibiotics were given in 28% of the patients, despite detection of virus. The rate of antibiotic prescription was higher among LRTI when compared with URSI (54%-21%; P = 0.003). The third generation cephalosporins, β-lactam/β-lactamase inhibitor combination, and quinolones were the most commonly used antibiotics (17%, 11%, and 10%, respectively). Finally, ribavirin was given to four patients, three of whom were severe and required respiratory support. None of the patients died of hMPV infection. Our study demonstrates the radiologic findings of hMPV infections in patients with LRSI (Figures 3 and 4) . One of the most interesting radiologic findings was the presence of nodular consolidation in CT, which is generally expected to be seen in bacterial infection. The PI in CR and GGO in CT are the most common radiologic findings, which are frequently seen in viral pneumonia. In a recent review for radiologic imaging of viral agents that may cause pneumonia, the general radiologic findings of hMPV infections were followed as bilateral centrilobular nodules, GGO, and multilobar infiltrations; however, there was no information about nodular consolidation. In addition, they noted that the radiologic findings of hMPV infections were most commonly reported in patients with hematologic malignancy, but there were limited data on immunocompetent patients. 9 The time from onset of symptoms to hospital admission may affect radiologic findings. In our study, among patients with normal CR, which were LRSI-based on their clinical findings, time from onset of symptoms to hospital admission was lower (2.5 to 3.6 days; P = 0.05 antibiotic prescription rate was still high, which was 54% among patients with LRSI. There is no confirmed antiviral treatment in hMPV infection. Ribavirin use in hMPV infections is limited to case reports in immunocompromised patients. 17 In a recent study, ribavirin use was not associated with a better clinical outcome. They reported that the duration of viral shedding was shorter in patients using ribavirin. 10 A recent review also concluded that there is no evidence to recommend ribavirin in immunocompromised patients. 19 The fourth European Conference on Infections in Leukemia (ECIL-4) guideline also commented that general recommendations for treatment of hMPV infection could not be made with current evidence. 20 In our study, ribavirin was used in four cases, three of whom were severe and required respiratory support and none of them died. 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