id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-352178-irjhmxsg	Saxton-Shaw, Kali D.	O'nyong nyong Virus Molecular Determinants of Unique Vector Specificity Reside in Non-Structural Protein 3	2013-01-24		.txt	text/plain	5953	299	49	Fifteen distinct chimeric viruses were constructed to evaluate both structural and non-structural regions of the genome and infection patterns were determined through artificial infectious feeds in An. gambiae with each of these chimeras. When ONNV non-structural protein 3 (nsP3) replaced nsP3 from CHIKV virus in one of the chimeric viruses, infection rates in An. gambiae went from 0% to 63.5%. Our study analyzed both structural and non-structural regions of the ONNV genome using chimeric viruses and artificially infected Anopheles gambiae mosquitoes. When ONNV non-structural protein 3 (nsP3) replaced nsP3 from chikungunya virus in one of the chimeric viruses, infection rates in An. gambiae went from 0% to 63.5%. Six additional non-structural chimeric viruses were also constructed using a novel type II restriction enzyme cloning strategy to examine the broader genome with respect to ONNV's unique vector specificity for An. gambiae mosquitoes (Figure 2) .	./cache/cord-352178-irjhmxsg.txt	./txt/cord-352178-irjhmxsg.txt