id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325172-a8ntxnmm	Yip, Ming Shum	Antibody-dependent infection of human macrophages by severe acute respiratory syndrome coronavirus	2014-05-06		.txt	text/plain	5791	253	44	More recently, we demonstrated that anti-Spike antibody potentiates infection of both monocytic and lymphoid immune cell lines, not only by SARS-CoVpp but also by replication-competent SARS-coronavirus [16] , thus providing evidence for a novel and versatile mechanism by which SARS-CoV can enter into target cells that do not express the conventional ACE2 virus receptor and are otherwise refractory to the virus. Finally, we have provided evidence that the intracellular signaling motifbut not the IgG binding motifof the FcγR is the key molecular determinant for triggering ADE of SARS-CoVpp. Our findings conclusively demonstrate that anti-spike serum promotes internalization of SARS-CoV by human macrophages. All the endodomain-truncated constructs (FcγRIIA-H.ΔIC, FcγRIIA-R.ΔIC and FcγRIIB.ΔIC, corresponding to constructs 2, 6, 11 respectively) were not susceptible to ADE of infection, indicating that binding of anti-Spike IgG-SARS-CoVpp immune complexes was not sufficient to mediate entry and that the signaling-competent endodomain was required.	./cache/cord-325172-a8ntxnmm.txt	./txt/cord-325172-a8ntxnmm.txt