id author title date pages extension mime words sentences flesch summary cache txt cord-257644-9f30s0gy Mu, Xingjiang Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila 2013-09-12 .txt text/plain 6265 321 54 title: Comparative transcriptional analysis reveals distinct expression patterns of channel catfish genes after the first infection and re-infection with Aeromonas hydrophila To determine whether transcriptional levels of channel catfish (Ictalurus punctatus) genes are differentially regulated between a first infection with Aeromonas hydrophila and a re-infection, suppression subtractive hybridization (SSH) was performed in this study using anterior kidney cDNA after the re-infection as tester. Of the 96 clones isolated from the SSH library, 28 unique expressed sequence tags (ESTs) were obtained, of which eight were confirmed to be slightly but significantly (P < 0.05) more up-regulated by the re-infection at 6 h post infection (hpi). hydrophila compared to that by a first infection, a total of 96 channel catfish genes reported in literature were screened in this study, including the following: 1) 43 genes induced by Edwardsiella ictaluri [24] ; 2) 28 genes upregulated by Flavobacterium columnare [25] ; 3) 5 toll-like receptors (TLRs) up-regulated by E. ./cache/cord-257644-9f30s0gy.txt ./txt/cord-257644-9f30s0gy.txt