id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-251991-ghbpga1s	Harcourt, Jennifer L.	Evaluation of the Calu-3 cell line as a model of in vitro respiratory syncytial virus infection()	2011-03-31		.txt	text/plain	3557	169	43	Respiratory syncytial virus (RSV) replication is primarily limited to the upper respiratory tract epithelium and primary, differentiated normal human bronchial epithelial cells (NHBE) have, therefore, been considered a good system for in vitro analysis of lung tissue response to respiratory virus infection and virus–host interactions. Polarized Calu-3 are susceptible to RSV infection and release infectious virus primarily from the apical surface, consistent with studies in NHBE cells. The mechanisms of cellular responses to RSV infection have been studied extensively in vitro in a variety of immortalized epithelial cell lines grown in monolayer cultures, including but not limited to Vero, Hep-2, A549, and ଝ The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of CDC. Consistent with previous studies in polarized MDCK (Roberts et al., 1995) and in differentiated NHBE, polarized Calu-3 released infectious virus primarily from the apical surface, and infection was persistent, detectable for at least 6 weeks post-infection.	./cache/cord-251991-ghbpga1s.txt	./txt/cord-251991-ghbpga1s.txt