id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-017959-g0nf1iwm	Lipkin, W. Ian	Diagnosis, Discovery and Dissection of Viral Diseases	2014-02-27		.txt	text/plain	5016	216	37	Nested PCR tests that can employ consensus or specifi c primers in two sequential amplifi cation reactions with either one (hemi-nested) or two (fully nested) primers located 3′ with respect to the fi rst primer set may both accommodate sequence variation and be more sensitive than fl uorescent or beacon-based singleplex assays. Whereas multiplex PCR systems support rapid highthroughput diagnosis with highest sensitivity for a limited number of agents, microarray-based systems provide detection of all known pathogens for which sequence information is available, but at the expense of some degree of sensitivity. Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fl uid microbead-based assay MassTag polymerasechain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused infl uenza-like illness in New York State during	./cache/cord-017959-g0nf1iwm.txt	./txt/cord-017959-g0nf1iwm.txt