id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-341478-bicg5ozr	Aurisicchio, L	Regulated and prolonged expression of mIFNα in immunocompetent mice mediated by a helper-dependent adenovirus vector	2002-01-30		.txt	text/plain	5934	296	53	To explore the regulated transgene expression in the context of a helper-dependent vector, we constructed the HD-TET-IFN vector, containing the mIFNα gene under the control of the tetracycline inducible transactivator rtTA2(s)-S2. In addition, C57/B6 mice injected at low HD-TET-IFN doses resulted in Doxmediated regulation of liver restricted mIFN␣ expression, which was associated with induction of antiviral genes. These results indicate that HD-TET-IFN allowed a significant expression of mIFN␣ controlled in vitro by Dox. To verify the efficiency and persistence of mIFN␣ secretion, C57/B6 mice were injected i.v. with 1.4 × 10 10 pp of HD-IFN or HD-TET-IFN and the mIFN␣ released in the serum was measured over time. C57/B6 and Balb/C mice were i.v. injected with different doses of HD-TET-IFN and mIFN␣ measured at day 21 p.i. after a treatment for 3 days with Dox, 200 g/ml, present in the drinking water (Figure 7a ).	./cache/cord-341478-bicg5ozr.txt	./txt/cord-341478-bicg5ozr.txt