id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-317573-wp2wr3b5	Peng, Hui	Human memory T cell responses to SARS-CoV E protein	2006-06-30		.txt	text/plain	4124	197	60	In this study, we found that peripheral blood mononuclear cells (PBMCs) from fully recovered SARS individuals rapidly produced IFN-γ and IL-2 following stimulation with a pool of 9 peptides overlapping the entire E protein sequence. To assess memory T cell response specific for E protein after SARS-CoV infection in humans, PBMCs from individuals who had fully recovered from SARS two years after infection were stimulated with a pool of 9 peptides spanning the entire amino acid sequence of the SARS-CoV E protein, or the cells were stimulated with anti-CD3 and anti-CD28 antibodies under the same culture conditions as positive controls. Similarly, the frequency of SARS-CoV E antigen-specific IFN-g-producing cells determined by IFN-g ELISPOT assay in PBMCs from 8 fully recovered SARS individuals was significantly higher than that of the cells from the normal donor controls in response to E peptides (Fig. 1B) .	./cache/cord-317573-wp2wr3b5.txt	./txt/cord-317573-wp2wr3b5.txt