id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299299-ov6bf1ff	Janowicz, Anna	Multiple Genome Segments Determine Virulence of Bluetongue Virus Serotype 8	2015-03-11		.txt	text/plain	7538	406	54	Replication of BTV8(H) was relatively poor in interferon (IFN)-competent primary ovine endothelial cells compared to replication of BTV8(L), and this phenotype was determined by several viral genomic segments, including Seg4 and Seg9. Here, in order to determine the roles played by specific BTV genomic segments in virus adaptation to tissue culture and attenuation in vivo, we compared genetic and phenotypic differences between BTV8 NET2006 minimally passaged in tissue culture, a derivative of this strain extensively passaged in cell culture, and 34 reassortants between the two viruses. Both viruses replicated very efficiently in IFN-deficient CPT-Tert cells, but BTV8 H reached titers approximately 100-fold higher than its parental virus (Fig. 1A) . The data obtained so far indicate that BTV8 H had accumulated mutations that affected virus replication in IFN-competent cells and resulted in attenuation in vivo both in IFNAR Ϫ/Ϫ mice and in sheep.	./cache/cord-299299-ov6bf1ff.txt	./txt/cord-299299-ov6bf1ff.txt