id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292593-apdyaujt	Coulter-Mackie, Marion	In vivo and in vitro models of demyelinating diseases XII. Persistence and expression of corona JHM vims functions in RN2-2 Schwannoma cells during latency	1985-10-31		.txt	text/plain	5832	299	55	Interference with vesicular stomatitis virus (VSV) production by JHMV was tested in persistently or latently infected RN-2 cells using as the controls, uninfected RN2-2 cells. Since upon infection of RN2-2 cultures with JHMV at 39.5"C and maintenance in a state of latency at that temperature for several days, the cells commenced yielding pfu upon shift down to 32.5"C (Lucas et al., 1978) , these data suggest that penetration and eclipse of the virus inoculum must have occurred normally at 39.5"C and restriction most probably developed at a subsequent stage. It is very significant that during incubation at the restrictive temperature for as long as 7 days and beyond, when RN2-2 cells had multiplied through 6-7 generations, the 56K antigen was readily detectable (channel I), implying that during latency, in the absence of infectious virus production, translation into JHMV nucleocapsid protein continued.	./cache/cord-292593-apdyaujt.txt	./txt/cord-292593-apdyaujt.txt