id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290396-cy4y8vnt	Kumar, Matam Vijay	Innate immunity in the retina: Toll-like receptor (TLR) signaling in human retinal pigment epithelial cells	2004-07-01		.txt	text/plain	5490	363	61	title: Innate immunity in the retina: Toll-like receptor (TLR) signaling in human retinal pigment epithelial cells We evaluated the expression and function of TLRs in human retinal pigment epithelial (RPE) cells. The presence of TLRs on RPE cells and the resultant TLR signaling in RPE cells suggest that these molecules may play an important role in innate and adaptive immune responses within the retina. Our data suggest that the binding of poly I:C, an analog of dsRNA, to TLR 3 on human RPE cells resulted in the production of IFN-h and other cytokines, chemokines, and adhesion molecules. Total RNA prepared from confluent monolayers of human RPE cells and from suspension cultures of U937 was used to evaluate the constitutive expression of TLR mRNA. To study the effects of TLR activators, human RPE cells were washed with serum-free media (SFM) and incubated in SFM for 12 h in the presence of poly I:C (100 Ag/ml ), LPS (5 Ag/ml), or IFN-g (100 U/ml).	./cache/cord-290396-cy4y8vnt.txt	./txt/cord-290396-cy4y8vnt.txt