id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273144-er6irjw3	Powell, Fiona	Development of reagents to study the turkey's immune response: Cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13	2012-06-15		.txt	text/plain	3956	246	58	The availability of genome sequences for other avian species, including the zebrafinch (Warren et al., 2010) , the turkey (Dalloul et al., 2010) and the duck (http://pre.ensembl.org/Anas platyrhyncos/Info/), should allow identification and subsequent characterisation of their immune gene repertoires. Real-time qRT-PCR was used to determine IFN-␥ mRNA expression levels in PHA-stimulated splenocytes (from chicken and turkey) treated with recombinant chicken or turkey IL-10 or mock-transfected COS cell supernatant at various dilutions. Similarly, there was inhibition of IFN-␥ production from PHA-stimulated turkey splenocytes by recombinant chicken and turkey IL-10, although Expression patterns of (A) IL-10 and (B) IL-13 mRNA, as measured by real-time quantitative RT-PCR, in lymphoid tissues, non-lymphoid tissues and digestive tissues of the turkey. At the protein level (72 h post-stimulation), recombinant chicken and turkey IL-10 inhibited the production of IFN-␥ by both chicken and turkey splenocytes at high concentrations, as measured by ELISA, and this effect titrated out with increasing dilution of recombinant IL-10 ( Fig. 3C and D respectively).	./cache/cord-273144-er6irjw3.txt	./txt/cord-273144-er6irjw3.txt