id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004341-co9s26x1	Thukral, Akanksha	A single dose polyanhydride-based nanovaccine against paratuberculosis infection	2020-02-14		.txt	text/plain	6736	375	43	Pre-challenge immune responses For Trial I (i.e., safety study), the T cell response was evaluated at 6 weeks post-vaccination by performing IFN-γ ELISA on spleen derived lymphocytes (described in Methods) (Supplemental Fig. 1 ). Post-challenge immune responses To evaluate T cell response in Trial I/safety study IFN-γ ELISA was performed and result of which depicted no significant differences in IFN-γ levels among the groups (Supplemental Fig. 2b ) while at 12 weeks post challenge Mycopar and PAN-lysate vaccinated animals showed significantly higher IFN-γ levels as compared with control animals given PBS (Supplemental Fig. 2c ). At 12 WPC, multiparametric flow cytometry analysis indicated that mice immunized with PAN-Cf elicited a significantly higher percent of antigen specific double cytokine (IFN-γ, TNF-α + ) and single cytokine (IFN-γ) producing CD8 + T cells compared with non-vaccinated and Mycopar® vaccinated mice (Fig. 4 ).	./cache/cord-004341-co9s26x1.txt	./txt/cord-004341-co9s26x1.txt