id sid tid token lemma pos cord-300104-855iw9wi 1 1 key key JJ cord-300104-855iw9wi 2 1 : : : cord-300104-855iw9wi 2 2 cord-300104 cord-300104 NN cord-300104-855iw9wi 2 3 - - HYPH cord-300104-855iw9wi 2 4 855iw9wi 855iw9wi CD cord-300104-855iw9wi 2 5 authors author NNS cord-300104-855iw9wi 2 6 : : : cord-300104-855iw9wi 2 7 Hennion Hennion NNP cord-300104-855iw9wi 2 8 , , , cord-300104-855iw9wi 2 9 Ruth Ruth NNP cord-300104-855iw9wi 2 10 M. M. NNP cord-300104-855iw9wi 2 11 ; ; : cord-300104-855iw9wi 2 12 Hill Hill NNP cord-300104-855iw9wi 2 13 , , , cord-300104-855iw9wi 2 14 Gillian Gillian NNP cord-300104-855iw9wi 2 15 title title NN cord-300104-855iw9wi 2 16 : : : cord-300104-855iw9wi 3 1 The the DT cord-300104-855iw9wi 3 2 Preparation Preparation NNP cord-300104-855iw9wi 3 3 of of IN cord-300104-855iw9wi 3 4 Chicken Chicken NNP cord-300104-855iw9wi 3 5 Kidney kidney NN cord-300104-855iw9wi 3 6 Cell Cell NNP cord-300104-855iw9wi 3 7 Cultures culture NNS cord-300104-855iw9wi 3 8 for for IN cord-300104-855iw9wi 3 9 Virus Virus NNP cord-300104-855iw9wi 3 10 Propagation Propagation NNP cord-300104-855iw9wi 3 11 date date NN cord-300104-855iw9wi 3 12 : : : cord-300104-855iw9wi 3 13 2014 2014 CD cord-300104-855iw9wi 3 14 - - SYM cord-300104-855iw9wi 3 15 12 12 CD cord-300104-855iw9wi 3 16 - - SYM cord-300104-855iw9wi 3 17 18 18 CD cord-300104-855iw9wi 3 18 journal journal NN cord-300104-855iw9wi 3 19 : : : cord-300104-855iw9wi 4 1 Coronaviruses Coronaviruses NNP cord-300104-855iw9wi 4 2 DOI DOI NNP cord-300104-855iw9wi 4 3 : : : cord-300104-855iw9wi 4 4 10.1007/978 10.1007/978 CD cord-300104-855iw9wi 4 5 - - HYPH cord-300104-855iw9wi 4 6 1 1 CD cord-300104-855iw9wi 4 7 - - HYPH cord-300104-855iw9wi 4 8 4939 4939 CD cord-300104-855iw9wi 4 9 - - HYPH cord-300104-855iw9wi 4 10 2438 2438 CD cord-300104-855iw9wi 4 11 - - HYPH cord-300104-855iw9wi 4 12 7_6 7_6 CD cord-300104-855iw9wi 5 1 sha sha NNP cord-300104-855iw9wi 5 2 : : : cord-300104-855iw9wi 6 1 69fa88ffea6e091803ed05debfc5f14578e36e9a 69fa88ffea6e091803ed05debfc5f14578e36e9a CD cord-300104-855iw9wi 6 2 doc_id doc_id CD cord-300104-855iw9wi 7 1 : : : cord-300104-855iw9wi 7 2 300104 300104 CD cord-300104-855iw9wi 7 3 cord_uid cord_uid NNS cord-300104-855iw9wi 7 4 : : : cord-300104-855iw9wi 8 1 855iw9wi 855iw9wi CD cord-300104-855iw9wi 8 2 Chicken chicken NN cord-300104-855iw9wi 8 3 kidney kidney NN cord-300104-855iw9wi 8 4 ( ( -LRB- cord-300104-855iw9wi 8 5 CK CK NNP cord-300104-855iw9wi 8 6 ) ) -RRB- cord-300104-855iw9wi 8 7 cell cell NN cord-300104-855iw9wi 8 8 cultures culture NNS cord-300104-855iw9wi 8 9 have have VBP cord-300104-855iw9wi 8 10 historically historically RB cord-300104-855iw9wi 8 11 proved prove VBN cord-300104-855iw9wi 8 12 useful useful JJ cord-300104-855iw9wi 8 13 for for IN cord-300104-855iw9wi 8 14 the the DT cord-300104-855iw9wi 8 15 assay assay NN cord-300104-855iw9wi 8 16 of of IN cord-300104-855iw9wi 8 17 a a DT cord-300104-855iw9wi 8 18 number number NN cord-300104-855iw9wi 8 19 of of IN cord-300104-855iw9wi 8 20 viruses virus NNS cord-300104-855iw9wi 8 21 including include VBG cord-300104-855iw9wi 8 22 coronaviruses coronaviruse NNS cord-300104-855iw9wi 8 23 . . . cord-300104-855iw9wi 9 1 A a DT cord-300104-855iw9wi 9 2 technique technique NN cord-300104-855iw9wi 9 3 for for IN cord-300104-855iw9wi 9 4 the the DT cord-300104-855iw9wi 9 5 preparation preparation NN cord-300104-855iw9wi 9 6 of of IN cord-300104-855iw9wi 9 7 such such JJ cord-300104-855iw9wi 9 8 cell cell NN cord-300104-855iw9wi 9 9 cultures culture NNS cord-300104-855iw9wi 9 10 , , , cord-300104-855iw9wi 9 11 using use VBG cord-300104-855iw9wi 9 12 a a DT cord-300104-855iw9wi 9 13 combination combination NN cord-300104-855iw9wi 9 14 of of IN cord-300104-855iw9wi 9 15 manual manual JJ cord-300104-855iw9wi 9 16 and and CC cord-300104-855iw9wi 9 17 trypsin trypsin JJ cord-300104-855iw9wi 9 18 disaggregation disaggregation NN cord-300104-855iw9wi 9 19 of of IN cord-300104-855iw9wi 9 20 kidneys kidney NNS cord-300104-855iw9wi 9 21 dissected dissect VBN cord-300104-855iw9wi 9 22 from from IN cord-300104-855iw9wi 9 23 2- 2- CD cord-300104-855iw9wi 9 24 to to IN cord-300104-855iw9wi 9 25 3-week 3-week JJ cord-300104-855iw9wi 9 26 - - JJ cord-300104-855iw9wi 9 27 old old JJ cord-300104-855iw9wi 9 28 birds bird NNS cord-300104-855iw9wi 9 29 is be VBZ cord-300104-855iw9wi 9 30 described describe VBN cord-300104-855iw9wi 9 31 . . . cord-300104-855iw9wi 10 1 This this DT cord-300104-855iw9wi 10 2 technique technique NN cord-300104-855iw9wi 10 3 routinely routinely RB cord-300104-855iw9wi 10 4 gives give VBZ cord-300104-855iw9wi 10 5 high high JJ cord-300104-855iw9wi 10 6 cell cell NN cord-300104-855iw9wi 10 7 yield yield NN cord-300104-855iw9wi 10 8 together together RB cord-300104-855iw9wi 10 9 with with IN cord-300104-855iw9wi 10 10 high high JJ cord-300104-855iw9wi 10 11 viability viability NN cord-300104-855iw9wi 10 12 and and CC cord-300104-855iw9wi 10 13 the the DT cord-300104-855iw9wi 10 14 resultant resultant JJ cord-300104-855iw9wi 10 15 adherent adherent JJ cord-300104-855iw9wi 10 16 primary primary JJ cord-300104-855iw9wi 10 17 cultures culture NNS cord-300104-855iw9wi 10 18 can can MD cord-300104-855iw9wi 10 19 be be VB cord-300104-855iw9wi 10 20 used use VBN cord-300104-855iw9wi 10 21 for for IN cord-300104-855iw9wi 10 22 virus virus NN cord-300104-855iw9wi 10 23 growth growth NN cord-300104-855iw9wi 10 24 and and CC cord-300104-855iw9wi 10 25 plaque plaque NN cord-300104-855iw9wi 10 26 formation formation NN cord-300104-855iw9wi 10 27 . . . cord-300104-855iw9wi 11 1 Techniques technique NNS cord-300104-855iw9wi 11 2 for for IN cord-300104-855iw9wi 11 3 the the DT cord-300104-855iw9wi 11 4 preparation preparation NN cord-300104-855iw9wi 11 5 of of IN cord-300104-855iw9wi 11 6 monolayer monolayer NN cord-300104-855iw9wi 11 7 cultures culture NNS cord-300104-855iw9wi 11 8 from from IN cord-300104-855iw9wi 11 9 adult adult NN cord-300104-855iw9wi 11 10 kidney kidney NN cord-300104-855iw9wi 11 11 cells cell NNS cord-300104-855iw9wi 11 12 suitable suitable JJ cord-300104-855iw9wi 11 13 for for IN cord-300104-855iw9wi 11 14 the the DT cord-300104-855iw9wi 11 15 growth growth NN cord-300104-855iw9wi 11 16 and and CC cord-300104-855iw9wi 11 17 quantitation quantitation NN cord-300104-855iw9wi 11 18 of of IN cord-300104-855iw9wi 11 19 viruses virus NNS cord-300104-855iw9wi 11 20 have have VBP cord-300104-855iw9wi 11 21 been be VBN cord-300104-855iw9wi 11 22 available available JJ cord-300104-855iw9wi 11 23 for for IN cord-300104-855iw9wi 11 24 many many JJ cord-300104-855iw9wi 11 25 years year NNS cord-300104-855iw9wi 11 26 ; ; : cord-300104-855iw9wi 11 27 Dulbecco Dulbecco NNP cord-300104-855iw9wi 11 28 and and CC cord-300104-855iw9wi 11 29 Vogt Vogt NNP cord-300104-855iw9wi 11 30 [ [ -LRB- cord-300104-855iw9wi 11 31 1 1 CD cord-300104-855iw9wi 11 32 ] ] -RRB- cord-300104-855iw9wi 11 33 described describe VBD cord-300104-855iw9wi 11 34 the the DT cord-300104-855iw9wi 11 35 preparation preparation NN cord-300104-855iw9wi 11 36 of of IN cord-300104-855iw9wi 11 37 Monkey Monkey NNP cord-300104-855iw9wi 11 38 Kidney kidney NN cord-300104-855iw9wi 11 39 cultures culture NNS cord-300104-855iw9wi 11 40 in in IN cord-300104-855iw9wi 11 41 1953 1953 CD cord-300104-855iw9wi 11 42 and and CC cord-300104-855iw9wi 11 43 Youngner Youngner NNP cord-300104-855iw9wi 11 44 [ [ -LRB- cord-300104-855iw9wi 11 45 2 2 CD cord-300104-855iw9wi 11 46 ] ] -RRB- cord-300104-855iw9wi 11 47 published publish VBD cord-300104-855iw9wi 11 48 a a DT cord-300104-855iw9wi 11 49 modifi modifi JJ cord-300104-855iw9wi 11 50 cation cation NN cord-300104-855iw9wi 11 51 of of IN cord-300104-855iw9wi 11 52 the the DT cord-300104-855iw9wi 11 53 process process NN cord-300104-855iw9wi 11 54 in in IN cord-300104-855iw9wi 11 55 1954 1954 CD cord-300104-855iw9wi 11 56 . . . cord-300104-855iw9wi 12 1 Maassab maassab RB cord-300104-855iw9wi 12 2 in in IN cord-300104-855iw9wi 12 3 1959 1959 CD cord-300104-855iw9wi 12 4 [ [ -LRB- cord-300104-855iw9wi 12 5 3 3 CD cord-300104-855iw9wi 12 6 ] ] -RRB- cord-300104-855iw9wi 12 7 describes describe VBZ cord-300104-855iw9wi 12 8 the the DT cord-300104-855iw9wi 12 9 preparation preparation NN cord-300104-855iw9wi 12 10 of of IN cord-300104-855iw9wi 12 11 Chicken Chicken NNP cord-300104-855iw9wi 12 12 Kidney kidney NN cord-300104-855iw9wi 12 13 monolayer monolayer NN cord-300104-855iw9wi 12 14 cultures culture NNS cord-300104-855iw9wi 12 15 from from IN cord-300104-855iw9wi 12 16 4-to 4-to CD cord-300104-855iw9wi 12 17 5-day 5-day JJ cord-300104-855iw9wi 12 18 - - HYPH cord-300104-855iw9wi 12 19 old old JJ cord-300104-855iw9wi 12 20 chicks chick NNS cord-300104-855iw9wi 12 21 , , , cord-300104-855iw9wi 12 22 the the DT cord-300104-855iw9wi 12 23 cultures culture NNS cord-300104-855iw9wi 12 24 being be VBG cord-300104-855iw9wi 12 25 used use VBN cord-300104-855iw9wi 12 26 for for IN cord-300104-855iw9wi 12 27 studies study NNS cord-300104-855iw9wi 12 28 with with IN cord-300104-855iw9wi 12 29 some some DT cord-300104-855iw9wi 12 30 human human JJ cord-300104-855iw9wi 12 31 viruses virus NNS cord-300104-855iw9wi 12 32 and and CC cord-300104-855iw9wi 12 33 Churchill Churchill NNP cord-300104-855iw9wi 12 34 [ [ -LRB- cord-300104-855iw9wi 12 35 4 4 CD cord-300104-855iw9wi 12 36 ] ] -RRB- cord-300104-855iw9wi 12 37 reports report VBZ cord-300104-855iw9wi 12 38 the the DT cord-300104-855iw9wi 12 39 use use NN cord-300104-855iw9wi 12 40 of of IN cord-300104-855iw9wi 12 41 chicken chicken NN cord-300104-855iw9wi 12 42 kidney kidney NN cord-300104-855iw9wi 12 43 tissue tissue NN cord-300104-855iw9wi 12 44 cultures culture NNS cord-300104-855iw9wi 12 45 derived derive VBN cord-300104-855iw9wi 12 46 from from IN cord-300104-855iw9wi 12 47 3 3 CD cord-300104-855iw9wi 12 48 to to TO cord-300104-855iw9wi 12 49 8 8 CD cord-300104-855iw9wi 12 50 week week NN cord-300104-855iw9wi 12 51 old old JJ cord-300104-855iw9wi 12 52 chickens chicken NNS cord-300104-855iw9wi 12 53 in in IN cord-300104-855iw9wi 12 54 the the DT cord-300104-855iw9wi 12 55 study study NN cord-300104-855iw9wi 12 56 of of IN cord-300104-855iw9wi 12 57 avian avian JJ cord-300104-855iw9wi 12 58 viruses virus NNS cord-300104-855iw9wi 12 59 including include VBG cord-300104-855iw9wi 12 60 Infectious Infectious NNP cord-300104-855iw9wi 12 61 Bronchitis Bronchitis NNP cord-300104-855iw9wi 12 62 Virus Virus NNP cord-300104-855iw9wi 12 63 ( ( -LRB- cord-300104-855iw9wi 12 64 IBV IBV NNP cord-300104-855iw9wi 12 65 ) ) -RRB- cord-300104-855iw9wi 12 66 . . . cord-300104-855iw9wi 13 1 The the DT cord-300104-855iw9wi 13 2 technique technique NN cord-300104-855iw9wi 13 3 for for IN cord-300104-855iw9wi 13 4 the the DT cord-300104-855iw9wi 13 5 production production NN cord-300104-855iw9wi 13 6 of of IN cord-300104-855iw9wi 13 7 kidney kidney NN cord-300104-855iw9wi 13 8 cell cell NN cord-300104-855iw9wi 13 9 monolayer monolayer NN cord-300104-855iw9wi 13 10 cultures culture NNS cord-300104-855iw9wi 13 11 from from IN cord-300104-855iw9wi 13 12 young young JJ cord-300104-855iw9wi 13 13 birds bird NNS cord-300104-855iw9wi 13 14 , , , cord-300104-855iw9wi 13 15 as as IN cord-300104-855iw9wi 13 16 described describe VBN cord-300104-855iw9wi 13 17 here here RB cord-300104-855iw9wi 13 18 , , , cord-300104-855iw9wi 13 19 is be VBZ cord-300104-855iw9wi 13 20 adapted adapt VBN cord-300104-855iw9wi 13 21 from from IN cord-300104-855iw9wi 13 22 those those DT cord-300104-855iw9wi 13 23 published publish VBN cord-300104-855iw9wi 13 24 by by IN cord-300104-855iw9wi 13 25 Dulbecco Dulbecco NNP cord-300104-855iw9wi 13 26 and and CC cord-300104-855iw9wi 13 27 Vogt Vogt NNP cord-300104-855iw9wi 13 28 [ [ -LRB- cord-300104-855iw9wi 13 29 1 1 CD cord-300104-855iw9wi 13 30 ] ] -RRB- cord-300104-855iw9wi 13 31 , , , cord-300104-855iw9wi 13 32 and and CC cord-300104-855iw9wi 13 33 Youngner Youngner NNP cord-300104-855iw9wi 13 34 [ [ -LRB- cord-300104-855iw9wi 13 35 2 2 CD cord-300104-855iw9wi 13 36 ] ] -RRB- cord-300104-855iw9wi 13 37 for for IN cord-300104-855iw9wi 13 38 monkey monkey NN cord-300104-855iw9wi 13 39 kidney kidney NN cord-300104-855iw9wi 13 40 cells cell NNS cord-300104-855iw9wi 13 41 . . . cord-300104-855iw9wi 14 1 Whilst whilst IN cord-300104-855iw9wi 14 2 titration titration NN cord-300104-855iw9wi 14 3 of of IN cord-300104-855iw9wi 14 4 IBV IBV NNP cord-300104-855iw9wi 14 5 in in IN cord-300104-855iw9wi 14 6 CK CK NNP cord-300104-855iw9wi 14 7 cells cell NNS cord-300104-855iw9wi 14 8 gives give VBZ cord-300104-855iw9wi 14 9 lower low JJR cord-300104-855iw9wi 14 10 titers titer NNS cord-300104-855iw9wi 14 11 than than IN cord-300104-855iw9wi 14 12 those those DT cord-300104-855iw9wi 14 13 obtained obtain VBN cord-300104-855iw9wi 14 14 in in IN cord-300104-855iw9wi 14 15 embryonated embryonate VBN cord-300104-855iw9wi 14 16 eggs egg NNS cord-300104-855iw9wi 14 17 [ [ -LRB- cord-300104-855iw9wi 14 18 5 5 CD cord-300104-855iw9wi 14 19 ] ] -RRB- cord-300104-855iw9wi 14 20 or or CC cord-300104-855iw9wi 14 21 tracheal tracheal JJ cord-300104-855iw9wi 14 22 organ organ NN cord-300104-855iw9wi 14 23 cultures culture NNS cord-300104-855iw9wi 14 24 [ [ -LRB- cord-300104-855iw9wi 14 25 6 6 CD cord-300104-855iw9wi 14 26 ] ] -RRB- cord-300104-855iw9wi 14 27 , , , cord-300104-855iw9wi 14 28 the the DT cord-300104-855iw9wi 14 29 ability ability NN cord-300104-855iw9wi 14 30 of of IN cord-300104-855iw9wi 14 31 CK CK NNP cord-300104-855iw9wi 14 32 cells cell NNS cord-300104-855iw9wi 14 33 to to TO cord-300104-855iw9wi 14 34 support support VB cord-300104-855iw9wi 14 35 the the DT cord-300104-855iw9wi 14 36 growth growth NN cord-300104-855iw9wi 14 37 of of IN cord-300104-855iw9wi 14 38 many many JJ cord-300104-855iw9wi 14 39 strains strain NNS cord-300104-855iw9wi 14 40 of of IN cord-300104-855iw9wi 14 41 IBV IBV NNP cord-300104-855iw9wi 14 42 is be VBZ cord-300104-855iw9wi 14 43 well well RB cord-300104-855iw9wi 14 44 proven prove VBN cord-300104-855iw9wi 14 45 . . . cord-300104-855iw9wi 15 1 Following follow VBG cord-300104-855iw9wi 15 2 adaptation adaptation NN cord-300104-855iw9wi 15 3 in in IN cord-300104-855iw9wi 15 4 embryonated embryonate VBN cord-300104-855iw9wi 15 5 eggs egg NNS cord-300104-855iw9wi 15 6 , , , cord-300104-855iw9wi 15 7 the the DT cord-300104-855iw9wi 15 8 Beaudette Beaudette NNP cord-300104-855iw9wi 15 9 strain strain NN cord-300104-855iw9wi 15 10 of of IN cord-300104-855iw9wi 15 11 IBV IBV NNP cord-300104-855iw9wi 15 12 produced produce VBD cord-300104-855iw9wi 15 13 characteristic characteristic JJ cord-300104-855iw9wi 15 14 cytopathic cytopathic JJ cord-300104-855iw9wi 15 15 effects effect NNS cord-300104-855iw9wi 15 16 ( ( -LRB- cord-300104-855iw9wi 15 17 CPE CPE NNP cord-300104-855iw9wi 15 18 ) ) -RRB- cord-300104-855iw9wi 15 19 on on IN cord-300104-855iw9wi 15 20 fi fi NNP cord-300104-855iw9wi 15 21 rst rst NN cord-300104-855iw9wi 15 22 passage passage NN cord-300104-855iw9wi 15 23 in in IN cord-300104-855iw9wi 15 24 CK CK NNP cord-300104-855iw9wi 15 25 cells cell NNS cord-300104-855iw9wi 15 26 , , , cord-300104-855iw9wi 15 27 whilst whilst IN cord-300104-855iw9wi 15 28 the the DT cord-300104-855iw9wi 15 29 Massachusetts Massachusetts NNP cord-300104-855iw9wi 15 30 strain strain NN cord-300104-855iw9wi 15 31 produced produce VBD cord-300104-855iw9wi 15 32 CPE CPE NNP cord-300104-855iw9wi 15 33 in in IN cord-300104-855iw9wi 15 34 the the DT cord-300104-855iw9wi 15 35 second second JJ cord-300104-855iw9wi 15 36 CK CK NNP cord-300104-855iw9wi 15 37 passage passage NN cord-300104-855iw9wi 15 38 [ [ -LRB- cord-300104-855iw9wi 15 39 4 4 CD cord-300104-855iw9wi 15 40 ] ] -RRB- cord-300104-855iw9wi 15 41 . . . cord-300104-855iw9wi 16 1 CPE CPE NNP cord-300104-855iw9wi 16 2 consists consist VBZ cord-300104-855iw9wi 16 3 of of IN cord-300104-855iw9wi 16 4 syncytia syncytia NN cord-300104-855iw9wi 16 5 formation formation NN cord-300104-855iw9wi 16 6 which which WDT cord-300104-855iw9wi 16 7 occurs occur VBZ cord-300104-855iw9wi 16 8 at at IN cord-300104-855iw9wi 16 9 6 6 CD cord-300104-855iw9wi 16 10 h h NN cord-300104-855iw9wi 16 11 post post NN cord-300104-855iw9wi 16 12 inoculation inoculation NN cord-300104-855iw9wi 16 13 with with IN cord-300104-855iw9wi 16 14 the the DT cord-300104-855iw9wi 16 15 Beaudette Beaudette NNP cord-300104-855iw9wi 16 16 strain strain NN cord-300104-855iw9wi 16 17 [ [ -LRB- cord-300104-855iw9wi 16 18 7 7 CD cord-300104-855iw9wi 16 19 ] ] -RRB- cord-300104-855iw9wi 16 20 . . . cord-300104-855iw9wi 17 1 The the DT cord-300104-855iw9wi 17 2 syncytia syncytia NN cord-300104-855iw9wi 17 3 may may MD cord-300104-855iw9wi 17 4 contain contain VB cord-300104-855iw9wi 17 5 as as RB cord-300104-855iw9wi 17 6 many many JJ cord-300104-855iw9wi 17 7 as as IN cord-300104-855iw9wi 17 8 20 20 CD cord-300104-855iw9wi 17 9 - - SYM cord-300104-855iw9wi 17 10 40 40 CD cord-300104-855iw9wi 17 11 or or CC cord-300104-855iw9wi 17 12 more more JJR cord-300104-855iw9wi 17 13 nuclei nucleus NNS cord-300104-855iw9wi 17 14 and and CC cord-300104-855iw9wi 17 15 they -PRON- PRP cord-300104-855iw9wi 17 16 quickly quickly RB cord-300104-855iw9wi 17 17 round round VBP cord-300104-855iw9wi 17 18 up up RP cord-300104-855iw9wi 17 19 and and CC cord-300104-855iw9wi 17 20 detach detach VB cord-300104-855iw9wi 17 21 from from IN cord-300104-855iw9wi 17 22 the the DT cord-300104-855iw9wi 17 23 culture culture NN cord-300104-855iw9wi 17 24 surface surface NN cord-300104-855iw9wi 17 25 . . . cord-300104-855iw9wi 18 1 Growth growth NN cord-300104-855iw9wi 18 2 curves curve NNS cord-300104-855iw9wi 18 3 of of IN cord-300104-855iw9wi 18 4 IBV IBV NNP cord-300104-855iw9wi 18 5 in in IN cord-300104-855iw9wi 18 6 CK CK NNP cord-300104-855iw9wi 18 7 cells cell NNS cord-300104-855iw9wi 18 8 show show VBP cord-300104-855iw9wi 18 9 a a DT cord-300104-855iw9wi 18 10 lag lag NN cord-300104-855iw9wi 18 11 phase phase NN cord-300104-855iw9wi 18 12 of of IN cord-300104-855iw9wi 18 13 2 2 CD cord-300104-855iw9wi 18 14 - - SYM cord-300104-855iw9wi 18 15 4 4 CD cord-300104-855iw9wi 18 16 h h NN cord-300104-855iw9wi 18 17 and and CC cord-300104-855iw9wi 18 18 maximum maximum JJ cord-300104-855iw9wi 18 19 virus virus NN cord-300104-855iw9wi 18 20 yield yield NN cord-300104-855iw9wi 18 21 in in IN cord-300104-855iw9wi 18 22 18 18 CD cord-300104-855iw9wi 18 23 - - SYM cord-300104-855iw9wi 18 24 20 20 CD cord-300104-855iw9wi 18 25 h h NN cord-300104-855iw9wi 18 26 [ [ -LRB- cord-300104-855iw9wi 18 27 5 5 CD cord-300104-855iw9wi 18 28 ] ] -RRB- cord-300104-855iw9wi 18 29 . . . cord-300104-855iw9wi 19 1 The the DT cord-300104-855iw9wi 19 2 ability ability NN cord-300104-855iw9wi 19 3 of of IN cord-300104-855iw9wi 19 4 CK CK NNP cord-300104-855iw9wi 19 5 cells cell NNS cord-300104-855iw9wi 19 6 to to TO cord-300104-855iw9wi 19 7 support support VB cord-300104-855iw9wi 19 8 the the DT cord-300104-855iw9wi 19 9 growth growth NN cord-300104-855iw9wi 19 10 of of IN cord-300104-855iw9wi 19 11 IBV IBV NNP cord-300104-855iw9wi 19 12 has have VBZ cord-300104-855iw9wi 19 13 been be VBN cord-300104-855iw9wi 19 14 utilized utilize VBN cord-300104-855iw9wi 19 15 in in IN cord-300104-855iw9wi 19 16 wide wide JJ cord-300104-855iw9wi 19 17 ranging range VBG cord-300104-855iw9wi 19 18 studies study NNS cord-300104-855iw9wi 19 19 including include VBG cord-300104-855iw9wi 19 20 the the DT cord-300104-855iw9wi 19 21 assessment assessment NN cord-300104-855iw9wi 19 22 of of IN cord-300104-855iw9wi 19 23 pH pH NNP cord-300104-855iw9wi 19 24 stability stability NN cord-300104-855iw9wi 19 25 of of IN cord-300104-855iw9wi 19 26 a a DT cord-300104-855iw9wi 19 27 series series NN cord-300104-855iw9wi 19 28 of of IN cord-300104-855iw9wi 19 29 IBV IBV NNP cord-300104-855iw9wi 19 30 strains strain NNS cord-300104-855iw9wi 19 31 [ [ -LRB- cord-300104-855iw9wi 19 32 8 8 CD cord-300104-855iw9wi 19 33 ] ] -RRB- cord-300104-855iw9wi 19 34 , , , cord-300104-855iw9wi 19 35 the the DT cord-300104-855iw9wi 19 36 identifi identifi NNP cord-300104-855iw9wi 19 37 cation cation NNP cord-300104-855iw9wi 19 38 of of IN cord-300104-855iw9wi 19 39 the the DT cord-300104-855iw9wi 19 40 presence presence NN cord-300104-855iw9wi 19 41 of of IN cord-300104-855iw9wi 19 42 a a DT cord-300104-855iw9wi 19 43 leader leader NN cord-300104-855iw9wi 19 44 sequence sequence NN cord-300104-855iw9wi 19 45 on on IN cord-300104-855iw9wi 19 46 IBV IBV NNP cord-300104-855iw9wi 19 47 mRNA mrna NN cord-300104-855iw9wi 20 1 A a DT cord-300104-855iw9wi 20 2 [ [ -LRB- cord-300104-855iw9wi 20 3 9 9 CD cord-300104-855iw9wi 20 4 ] ] -RRB- cord-300104-855iw9wi 20 5 , , , cord-300104-855iw9wi 20 6 the the DT cord-300104-855iw9wi 20 7 demonstration demonstration NN cord-300104-855iw9wi 20 8 that that WDT cord-300104-855iw9wi 20 9 the the DT cord-300104-855iw9wi 20 10 spike spike NN cord-300104-855iw9wi 20 11 protein protein NN cord-300104-855iw9wi 20 12 of of IN cord-300104-855iw9wi 20 13 IBV IBV NNP cord-300104-855iw9wi 20 14 is be VBZ cord-300104-855iw9wi 20 15 a a DT cord-300104-855iw9wi 20 16 determinant determinant NN cord-300104-855iw9wi 20 17 of of IN cord-300104-855iw9wi 20 18 cell cell NN cord-300104-855iw9wi 20 19 tropism tropism NN cord-300104-855iw9wi 20 20 [ [ -LRB- cord-300104-855iw9wi 20 21 10 10 CD cord-300104-855iw9wi 20 22 ] ] -RRB- cord-300104-855iw9wi 20 23 , , , cord-300104-855iw9wi 20 24 the the DT cord-300104-855iw9wi 20 25 induction induction NN cord-300104-855iw9wi 20 26 of of IN cord-300104-855iw9wi 20 27 protective protective JJ cord-300104-855iw9wi 20 28 immunity immunity NN cord-300104-855iw9wi 20 29 with with IN cord-300104-855iw9wi 20 30 recombinant recombinant JJ cord-300104-855iw9wi 20 31 IBV IBV NNP cord-300104-855iw9wi 20 32 Beaudette Beaudette NNP cord-300104-855iw9wi 20 33 [ [ -LRB- cord-300104-855iw9wi 20 34 11 11 CD cord-300104-855iw9wi 20 35 ] ] -RRB- cord-300104-855iw9wi 20 36 through through IN cord-300104-855iw9wi 20 37 to to IN cord-300104-855iw9wi 20 38 the the DT cord-300104-855iw9wi 20 39 identifi identifi NNP cord-300104-855iw9wi 20 40 cation cation NNP cord-300104-855iw9wi 20 41 of of IN cord-300104-855iw9wi 20 42 novel novel NNP cord-300104-855iw9wi 20 43 zippered zippered JJ cord-300104-855iw9wi 20 44 ER er NN cord-300104-855iw9wi 20 45 and and CC cord-300104-855iw9wi 20 46 associated associate VBD cord-300104-855iw9wi 20 47 spherules spherule NNS cord-300104-855iw9wi 20 48 induced induce VBN cord-300104-855iw9wi 20 49 by by IN cord-300104-855iw9wi 20 50 IBV IBV NNP cord-300104-855iw9wi 20 51 [ [ -LRB- cord-300104-855iw9wi 20 52 12 12 CD cord-300104-855iw9wi 20 53 ] ] -RRB- cord-300104-855iw9wi 20 54 . . . cord-300104-855iw9wi 21 1 Week week NN cord-300104-855iw9wi 21 2 old old JJ cord-300104-855iw9wi 21 3 chicken(s chicken(s NN cord-300104-855iw9wi 21 4 ) ) -RRB- cord-300104-855iw9wi 21 5 from from IN cord-300104-855iw9wi 21 6 specifi specifi NNP cord-300104-855iw9wi 21 7 c c NNP cord-300104-855iw9wi 21 8 pathogen pathogen NN cord-300104-855iw9wi 21 9 free free JJ cord-300104-855iw9wi 21 10 ( ( -LRB- cord-300104-855iw9wi 21 11 SPF SPF NNP cord-300104-855iw9wi 21 12 ) ) -RRB- cord-300104-855iw9wi 21 13 fl fl NNP cord-300104-855iw9wi 21 14 ock ock NNP cord-300104-855iw9wi 21 15 killed kill VBN cord-300104-855iw9wi 21 16 by by IN cord-300104-855iw9wi 21 17 cervical cervical JJ cord-300104-855iw9wi 21 18 dislocation dislocation NN cord-300104-855iw9wi 21 19 ( ( -LRB- cord-300104-855iw9wi 21 20 see see VB cord-300104-855iw9wi 21 21 Note Note NNP cord-300104-855iw9wi 21 22 1 1 CD cord-300104-855iw9wi 21 23 ) ) -RRB- cord-300104-855iw9wi 21 24 . . . cord-300104-855iw9wi 22 1 13 13 CD cord-300104-855iw9wi 22 2 . . . cord-300104-855iw9wi 23 1 Growth growth NN cord-300104-855iw9wi 23 2 medium medium NN cord-300104-855iw9wi 23 3 : : : cord-300104-855iw9wi 23 4 EMEM EMEM NNP cord-300104-855iw9wi 23 5 , , , cord-300104-855iw9wi 23 6 10 10 CD cord-300104-855iw9wi 23 7 % % NN cord-300104-855iw9wi 23 8 NBBS NBBS NNP cord-300104-855iw9wi 23 9 , , , cord-300104-855iw9wi 23 10 2.95 2.95 CD cord-300104-855iw9wi 23 11 g g NN cord-300104-855iw9wi 23 12 / / SYM cord-300104-855iw9wi 23 13 L L NNP cord-300104-855iw9wi 23 14 TPB TPB NNP cord-300104-855iw9wi 23 15 , , , cord-300104-855iw9wi 23 16 10 10 CD cord-300104-855iw9wi 23 17 mM mm CD cord-300104-855iw9wi 23 18 HEPES HEPES NNP cord-300104-855iw9wi 23 19 , , , cord-300104-855iw9wi 24 1 100 100 CD cord-300104-855iw9wi 24 2 U u NN cord-300104-855iw9wi 24 3 / / SYM cord-300104-855iw9wi 24 4 ml ml CC cord-300104-855iw9wi 24 5 penicillin penicillin NN cord-300104-855iw9wi 24 6 and and CC cord-300104-855iw9wi 24 7 100 100 CD cord-300104-855iw9wi 24 8 U U NNP cord-300104-855iw9wi 24 9 / / SYM cord-300104-855iw9wi 24 10 ml ml NNS cord-300104-855iw9wi 24 11 streptomycin streptomycin NNS cord-300104-855iw9wi 24 12 . . . cord-300104-855iw9wi 25 1 The the DT cord-300104-855iw9wi 25 2 number number NN cord-300104-855iw9wi 25 3 of of IN cord-300104-855iw9wi 25 4 kidney kidney NN cord-300104-855iw9wi 25 5 cells cell NNS cord-300104-855iw9wi 25 6 obtained obtain VBN cord-300104-855iw9wi 25 7 from from IN cord-300104-855iw9wi 25 8 each each DT cord-300104-855iw9wi 25 9 bird bird NN cord-300104-855iw9wi 25 10 will will MD cord-300104-855iw9wi 25 11 vary vary VB cord-300104-855iw9wi 25 12 with with IN cord-300104-855iw9wi 25 13 the the DT cord-300104-855iw9wi 25 14 age age NN cord-300104-855iw9wi 25 15 and and CC cord-300104-855iw9wi 25 16 the the DT cord-300104-855iw9wi 25 17 strain strain NN cord-300104-855iw9wi 25 18 of of IN cord-300104-855iw9wi 25 19 the the DT cord-300104-855iw9wi 25 20 birds bird NNS cord-300104-855iw9wi 25 21 used use VBN cord-300104-855iw9wi 25 22 . . . cord-300104-855iw9wi 26 1 We -PRON- PRP cord-300104-855iw9wi 26 2 have have VBP cord-300104-855iw9wi 26 3 found find VBN cord-300104-855iw9wi 26 4 that that IN cord-300104-855iw9wi 26 5 the the DT cord-300104-855iw9wi 26 6 average average JJ cord-300104-855iw9wi 26 7 cell cell NN cord-300104-855iw9wi 26 8 yield yield NN cord-300104-855iw9wi 26 9 from from IN cord-300104-855iw9wi 26 10 a a DT cord-300104-855iw9wi 26 11 2 2 CD cord-300104-855iw9wi 26 12 week week NN cord-300104-855iw9wi 26 13 old old JJ cord-300104-855iw9wi 26 14 Rhode Rhode NNP cord-300104-855iw9wi 26 15 Island Island NNP cord-300104-855iw9wi 26 16 Red Red NNP cord-300104-855iw9wi 26 17 bird bird NN cord-300104-855iw9wi 26 18 is be VBZ cord-300104-855iw9wi 26 19 approximately approximately RB cord-300104-855iw9wi 26 20 2.0 2.0 CD cord-300104-855iw9wi 26 21 × × CD cord-300104-855iw9wi 26 22 10 10 CD cord-300104-855iw9wi 26 23 8 8 CD cord-300104-855iw9wi 26 24 cells cell NNS cord-300104-855iw9wi 26 25 . . . cord-300104-855iw9wi 27 1 1 1 LS cord-300104-855iw9wi 27 2 . . . cord-300104-855iw9wi 28 1 Aseptically aseptically RB cord-300104-855iw9wi 28 2 prepare prepare VB cord-300104-855iw9wi 28 3 disaggregation disaggregation NN cord-300104-855iw9wi 28 4 mix mix NN cord-300104-855iw9wi 28 5 by by IN cord-300104-855iw9wi 28 6 addition addition NN cord-300104-855iw9wi 28 7 of of IN cord-300104-855iw9wi 28 8 7.5 7.5 CD cord-300104-855iw9wi 28 9 ml ml NNS cord-300104-855iw9wi 28 10 of of IN cord-300104-855iw9wi 28 11 trypsin trypsin NN cord-300104-855iw9wi 28 12 to to IN cord-300104-855iw9wi 28 13 80 80 CD cord-300104-855iw9wi 28 14 ml ml NNS cord-300104-855iw9wi 28 15 of of IN cord-300104-855iw9wi 28 16 EDTA EDTA NNP cord-300104-855iw9wi 28 17 and and CC cord-300104-855iw9wi 28 18 warm warm JJ cord-300104-855iw9wi 28 19 to to IN cord-300104-855iw9wi 28 20 37 37 CD cord-300104-855iw9wi 28 21 ° ° NN cord-300104-855iw9wi 28 22 C c NN cord-300104-855iw9wi 28 23 ( ( -LRB- cord-300104-855iw9wi 28 24 Trypsin/ Trypsin/ NNP cord-300104-855iw9wi 28 25 EDTA EDTA NNP cord-300104-855iw9wi 28 26 ) ) -RRB- cord-300104-855iw9wi 28 27 . . . cord-300104-855iw9wi 29 1 2 2 LS cord-300104-855iw9wi 29 2 . . . cord-300104-855iw9wi 30 1 Add add VB cord-300104-855iw9wi 30 2 50 50 CD cord-300104-855iw9wi 30 3 - - SYM cord-300104-855iw9wi 30 4 100 100 CD cord-300104-855iw9wi 30 5 ml ml NN cord-300104-855iw9wi 30 6 NBBS NBBS NNP cord-300104-855iw9wi 30 7 to to TO cord-300104-855iw9wi 30 8 suitable suitable JJ cord-300104-855iw9wi 30 9 sterile sterile JJ cord-300104-855iw9wi 30 10 fl fl NNP cord-300104-855iw9wi 30 11 ask ask NN cord-300104-855iw9wi 30 12 ( ( -LRB- cord-300104-855iw9wi 30 13 see see VB cord-300104-855iw9wi 30 14 Note Note NNP cord-300104-855iw9wi 30 15 5 5 CD cord-300104-855iw9wi 30 16 ) ) -RRB- cord-300104-855iw9wi 30 17 . . . cord-300104-855iw9wi 31 1 3 3 LS cord-300104-855iw9wi 31 2 . . . cord-300104-855iw9wi 32 1 Spray spray NN cord-300104-855iw9wi 32 2 work work NN cord-300104-855iw9wi 32 3 area area NN cord-300104-855iw9wi 32 4 with with IN cord-300104-855iw9wi 32 5 IMS IMS NNP cord-300104-855iw9wi 32 6 , , , cord-300104-855iw9wi 32 7 protect protect VB cord-300104-855iw9wi 32 8 with with IN cord-300104-855iw9wi 32 9 clean clean JJ cord-300104-855iw9wi 32 10 paper paper NN cord-300104-855iw9wi 32 11 towels towel NNS cord-300104-855iw9wi 32 12 and and CC cord-300104-855iw9wi 32 13 collect collect VB cord-300104-855iw9wi 32 14 sterile sterile JJ cord-300104-855iw9wi 32 15 instruments instrument NNS cord-300104-855iw9wi 32 16 and and CC cord-300104-855iw9wi 32 17 glassware glassware NN cord-300104-855iw9wi 32 18 so so IN cord-300104-855iw9wi 32 19 that that IN cord-300104-855iw9wi 32 20 they -PRON- PRP cord-300104-855iw9wi 32 21 are be VBP cord-300104-855iw9wi 32 22 close close JJ cord-300104-855iw9wi 32 23 to to TO cord-300104-855iw9wi 32 24 hand hand NN cord-300104-855iw9wi 32 25 . . . cord-300104-855iw9wi 33 1 4 4 LS cord-300104-855iw9wi 33 2 . . . cord-300104-855iw9wi 34 1 Spray spray VB cord-300104-855iw9wi 34 2 the the DT cord-300104-855iw9wi 34 3 back back NN cord-300104-855iw9wi 34 4 of of IN cord-300104-855iw9wi 34 5 the the DT cord-300104-855iw9wi 34 6 birds bird NNS cord-300104-855iw9wi 34 7 and and CC cord-300104-855iw9wi 34 8 under under IN cord-300104-855iw9wi 34 9 the the DT cord-300104-855iw9wi 34 10 wings wing NNS cord-300104-855iw9wi 34 11 with with IN cord-300104-855iw9wi 34 12 IMS IMS NNP cord-300104-855iw9wi 34 13 to to TO cord-300104-855iw9wi 34 14 clean clean VB cord-300104-855iw9wi 34 15 and and CC cord-300104-855iw9wi 34 16 dampen dampen VB cord-300104-855iw9wi 34 17 the the DT cord-300104-855iw9wi 34 18 feathers feather NNS cord-300104-855iw9wi 34 19 and and CC cord-300104-855iw9wi 34 20 lay lie VBD cord-300104-855iw9wi 34 21 the the DT cord-300104-855iw9wi 34 22 bird bird NN cord-300104-855iw9wi 34 23 , , , cord-300104-855iw9wi 34 24 dorsal dorsal JJ cord-300104-855iw9wi 34 25 side side NN cord-300104-855iw9wi 34 26 uppermost uppermost JJ cord-300104-855iw9wi 34 27 , , , cord-300104-855iw9wi 34 28 on on IN cord-300104-855iw9wi 34 29 the the DT cord-300104-855iw9wi 34 30 paper paper NN cord-300104-855iw9wi 34 31 towels towel NNS cord-300104-855iw9wi 34 32 . . . cord-300104-855iw9wi 35 1 5 5 LS cord-300104-855iw9wi 35 2 . . . cord-300104-855iw9wi 36 1 Insert insert VB cord-300104-855iw9wi 36 2 the the DT cord-300104-855iw9wi 36 3 blade blade NN cord-300104-855iw9wi 36 4 of of IN cord-300104-855iw9wi 36 5 a a DT cord-300104-855iw9wi 36 6 large large JJ cord-300104-855iw9wi 36 7 , , , cord-300104-855iw9wi 36 8 robust robust JJ cord-300104-855iw9wi 36 9 pair pair NN cord-300104-855iw9wi 36 10 of of IN cord-300104-855iw9wi 36 11 scissors scissor NNS cord-300104-855iw9wi 36 12 just just RB cord-300104-855iw9wi 36 13 below below RB cord-300104-855iw9wi 36 14 where where WRB cord-300104-855iw9wi 36 15 a a DT cord-300104-855iw9wi 36 16 wing wing NN cord-300104-855iw9wi 36 17 attaches attach VBZ cord-300104-855iw9wi 36 18 to to IN cord-300104-855iw9wi 36 19 the the DT cord-300104-855iw9wi 36 20 body body NN cord-300104-855iw9wi 36 21 and and CC cord-300104-855iw9wi 36 22 sever sever VB cord-300104-855iw9wi 36 23 across across IN cord-300104-855iw9wi 36 24 the the DT cord-300104-855iw9wi 36 25 body body NN cord-300104-855iw9wi 36 26 , , , cord-300104-855iw9wi 36 27 through through IN cord-300104-855iw9wi 36 28 the the DT cord-300104-855iw9wi 36 29 spinal spinal JJ cord-300104-855iw9wi 36 30 cord cord NN cord-300104-855iw9wi 36 31 to to IN cord-300104-855iw9wi 36 32 where where WRB cord-300104-855iw9wi 36 33 the the DT cord-300104-855iw9wi 36 34 second second JJ cord-300104-855iw9wi 36 35 wing wing NN cord-300104-855iw9wi 36 36 attaches attach VBZ cord-300104-855iw9wi 36 37 , , , cord-300104-855iw9wi 36 38 taking take VBG cord-300104-855iw9wi 36 39 care care NN cord-300104-855iw9wi 36 40 to to TO cord-300104-855iw9wi 36 41 avoid avoid VB cord-300104-855iw9wi 36 42 piercing pierce VBG cord-300104-855iw9wi 36 43 the the DT cord-300104-855iw9wi 36 44 gut gut NN cord-300104-855iw9wi 36 45 . . . cord-300104-855iw9wi 37 1 6 6 LS cord-300104-855iw9wi 37 2 . . . cord-300104-855iw9wi 38 1 From from IN cord-300104-855iw9wi 38 2 the the DT cord-300104-855iw9wi 38 3 ends end NNS cord-300104-855iw9wi 38 4 of of IN cord-300104-855iw9wi 38 5 this this DT cord-300104-855iw9wi 38 6 fi fi NNP cord-300104-855iw9wi 38 7 rst rst NNP cord-300104-855iw9wi 38 8 cut cut NN cord-300104-855iw9wi 38 9 , , , cord-300104-855iw9wi 38 10 once once RB cord-300104-855iw9wi 38 11 again again RB cord-300104-855iw9wi 38 12 taking take VBG cord-300104-855iw9wi 38 13 care care NN cord-300104-855iw9wi 38 14 to to TO cord-300104-855iw9wi 38 15 avoid avoid VB cord-300104-855iw9wi 38 16 piercing pierce VBG cord-300104-855iw9wi 38 17 the the DT cord-300104-855iw9wi 38 18 gut gut NN cord-300104-855iw9wi 38 19 and and CC cord-300104-855iw9wi 38 20 your -PRON- PRP$ cord-300104-855iw9wi 38 21 own own JJ cord-300104-855iw9wi 38 22 hand hand NN cord-300104-855iw9wi 38 23 , , , cord-300104-855iw9wi 38 24 cut cut VBN cord-300104-855iw9wi 38 25 along along IN cord-300104-855iw9wi 38 26 each each DT cord-300104-855iw9wi 38 27 side side NN cord-300104-855iw9wi 38 28 of of IN cord-300104-855iw9wi 38 29 the the DT cord-300104-855iw9wi 38 30 body body NN cord-300104-855iw9wi 38 31 towards towards IN cord-300104-855iw9wi 38 32 the the DT cord-300104-855iw9wi 38 33 legs leg NNS cord-300104-855iw9wi 38 34 and and CC cord-300104-855iw9wi 38 35 through through IN cord-300104-855iw9wi 38 36 the the DT cord-300104-855iw9wi 38 37 top top NN cord-300104-855iw9wi 38 38 of of IN cord-300104-855iw9wi 38 39 each each DT cord-300104-855iw9wi 38 40 leg leg NN cord-300104-855iw9wi 38 41 . . . cord-300104-855iw9wi 39 1 7 7 CD cord-300104-855iw9wi 39 2 . . . cord-300104-855iw9wi 40 1 Carefully carefully RB cord-300104-855iw9wi 40 2 fold fold VB cord-300104-855iw9wi 40 3 back back RB cord-300104-855iw9wi 40 4 the the DT cord-300104-855iw9wi 40 5 cut cut NN cord-300104-855iw9wi 40 6 section section NN cord-300104-855iw9wi 40 7 of of IN cord-300104-855iw9wi 40 8 the the DT cord-300104-855iw9wi 40 9 bird bird NN cord-300104-855iw9wi 40 10 to to TO cord-300104-855iw9wi 40 11 reveal reveal VB cord-300104-855iw9wi 40 12 the the DT cord-300104-855iw9wi 40 13 internal internal JJ cord-300104-855iw9wi 40 14 organs organ NNS cord-300104-855iw9wi 40 15 . . . cord-300104-855iw9wi 41 1 Move move VB cord-300104-855iw9wi 41 2 the the DT cord-300104-855iw9wi 41 3 intestines intestine NNS cord-300104-855iw9wi 41 4 , , , cord-300104-855iw9wi 41 5 which which WDT cord-300104-855iw9wi 41 6 should should MD cord-300104-855iw9wi 41 7 have have VB cord-300104-855iw9wi 41 8 remained remain VBN cord-300104-855iw9wi 41 9 attached attach VBN cord-300104-855iw9wi 41 10 to to IN cord-300104-855iw9wi 41 11 this this DT cord-300104-855iw9wi 41 12 retracted retract VBN cord-300104-855iw9wi 41 13 section section NN cord-300104-855iw9wi 41 14 , , , cord-300104-855iw9wi 41 15 to to TO cord-300104-855iw9wi 41 16 reveal reveal VB cord-300104-855iw9wi 41 17 the the DT cord-300104-855iw9wi 41 18 kidneys kidney NNS cord-300104-855iw9wi 41 19 . . . cord-300104-855iw9wi 42 1 8 8 CD cord-300104-855iw9wi 42 2 . . . cord-300104-855iw9wi 43 1 Remove remove VB cord-300104-855iw9wi 43 2 the the DT cord-300104-855iw9wi 43 3 kidneys kidney NNS cord-300104-855iw9wi 43 4 using use VBG cord-300104-855iw9wi 43 5 a a DT cord-300104-855iw9wi 43 6 small small JJ cord-300104-855iw9wi 43 7 pair pair NN cord-300104-855iw9wi 43 8 of of IN cord-300104-855iw9wi 43 9 scissors scissor NNS cord-300104-855iw9wi 43 10 and and CC cord-300104-855iw9wi 43 11 forceps forceps NNS cord-300104-855iw9wi 43 12 and and CC cord-300104-855iw9wi 43 13 place place NN cord-300104-855iw9wi 43 14 in in IN cord-300104-855iw9wi 43 15 a a DT cord-300104-855iw9wi 43 16 250 250 CD cord-300104-855iw9wi 43 17 ml ml NN cord-300104-855iw9wi 43 18 beaker beaker NN cord-300104-855iw9wi 43 19 of of IN cord-300104-855iw9wi 43 20 PBSa PBSa NNS cord-300104-855iw9wi 43 21 . . . cord-300104-855iw9wi 44 1 9 9 LS cord-300104-855iw9wi 44 2 . . . cord-300104-855iw9wi 45 1 Repeat repeat NN cord-300104-855iw9wi 45 2 steps step NNS cord-300104-855iw9wi 45 3 4 4 CD cord-300104-855iw9wi 45 4 -8 -8 NNS cord-300104-855iw9wi 45 5 for for IN cord-300104-855iw9wi 45 6 every every DT cord-300104-855iw9wi 45 7 bird bird NN cord-300104-855iw9wi 45 8 . . . cord-300104-855iw9wi 46 1 10 10 CD cord-300104-855iw9wi 46 2 . . . cord-300104-855iw9wi 47 1 When when WRB cord-300104-855iw9wi 47 2 all all PDT cord-300104-855iw9wi 47 3 the the DT cord-300104-855iw9wi 47 4 kidneys kidney NNS cord-300104-855iw9wi 47 5 required require VBN cord-300104-855iw9wi 47 6 have have VBP cord-300104-855iw9wi 47 7 been be VBN cord-300104-855iw9wi 47 8 removed remove VBN cord-300104-855iw9wi 47 9 from from IN cord-300104-855iw9wi 47 10 the the DT cord-300104-855iw9wi 47 11 birds bird NNS cord-300104-855iw9wi 47 12 , , , cord-300104-855iw9wi 47 13 agitate agitate VB cord-300104-855iw9wi 47 14 them -PRON- PRP cord-300104-855iw9wi 47 15 in in IN cord-300104-855iw9wi 47 16 the the DT cord-300104-855iw9wi 47 17 beaker beaker NN cord-300104-855iw9wi 47 18 and and CC cord-300104-855iw9wi 47 19 discard discard VB cord-300104-855iw9wi 47 20 the the DT cord-300104-855iw9wi 47 21 PBSa PBSa NNS cord-300104-855iw9wi 47 22 . . . cord-300104-855iw9wi 48 1 Repeat repeat VB cord-300104-855iw9wi 48 2 this this DT cord-300104-855iw9wi 48 3 process process NN cord-300104-855iw9wi 48 4 until until IN cord-300104-855iw9wi 48 5 the the DT cord-300104-855iw9wi 48 6 wash wash NNP cord-300104-855iw9wi 48 7 PBSa PBSa NNP cord-300104-855iw9wi 48 8 looks look VBZ cord-300104-855iw9wi 48 9 clear clear JJ cord-300104-855iw9wi 48 10 ( ( -LRB- cord-300104-855iw9wi 48 11 see see VB cord-300104-855iw9wi 48 12 Note Note NNP cord-300104-855iw9wi 48 13 6 6 CD cord-300104-855iw9wi 48 14 ) ) -RRB- cord-300104-855iw9wi 48 15 . . . cord-300104-855iw9wi 49 1 11 11 CD cord-300104-855iw9wi 49 2 . . . cord-300104-855iw9wi 50 1 Tip tip VB cord-300104-855iw9wi 50 2 the the DT cord-300104-855iw9wi 50 3 drained drain VBN cord-300104-855iw9wi 50 4 kidneys kidney NNS cord-300104-855iw9wi 50 5 into into IN cord-300104-855iw9wi 50 6 a a DT cord-300104-855iw9wi 50 7 large large JJ cord-300104-855iw9wi 50 8 glass glass NN cord-300104-855iw9wi 50 9 petri petri NN cord-300104-855iw9wi 50 10 dish dish NN cord-300104-855iw9wi 50 11 and and CC cord-300104-855iw9wi 50 12 using use VBG cord-300104-855iw9wi 50 13 two two CD cord-300104-855iw9wi 50 14 scalpels scalpel NNS cord-300104-855iw9wi 50 15 shred shre VBN cord-300104-855iw9wi 50 16 and and CC cord-300104-855iw9wi 50 17 mince mince VB cord-300104-855iw9wi 50 18 the the DT cord-300104-855iw9wi 50 19 kidneys kidney NNS cord-300104-855iw9wi 50 20 into into IN cord-300104-855iw9wi 50 21 very very RB cord-300104-855iw9wi 50 22 small small JJ cord-300104-855iw9wi 50 23 pieces piece NNS cord-300104-855iw9wi 50 24 removing remove VBG cord-300104-855iw9wi 50 25 as as RB cord-300104-855iw9wi 50 26 much much JJ cord-300104-855iw9wi 50 27 clotted clotted JJ cord-300104-855iw9wi 50 28 blood blood NN cord-300104-855iw9wi 50 29 , , , cord-300104-855iw9wi 50 30 connective connective JJ cord-300104-855iw9wi 50 31 tissue tissue NN cord-300104-855iw9wi 50 32 , , , cord-300104-855iw9wi 50 33 and and CC cord-300104-855iw9wi 50 34 kidney kidney NN cord-300104-855iw9wi 50 35 core core NN cord-300104-855iw9wi 50 36 as as IN cord-300104-855iw9wi 50 37 possible possible JJ cord-300104-855iw9wi 50 38 . . . cord-300104-855iw9wi 51 1 12 12 CD cord-300104-855iw9wi 51 2 . . . cord-300104-855iw9wi 52 1 Transfer transfer VB cord-300104-855iw9wi 52 2 the the DT cord-300104-855iw9wi 52 3 minced mince VBN cord-300104-855iw9wi 52 4 tissue tissue NN cord-300104-855iw9wi 52 5 into into IN cord-300104-855iw9wi 52 6 a a DT cord-300104-855iw9wi 52 7 tightly tightly RB cord-300104-855iw9wi 52 8 capped capped JJ cord-300104-855iw9wi 52 9 bottle bottle NN cord-300104-855iw9wi 52 10 ( ( -LRB- cord-300104-855iw9wi 52 11 for for IN cord-300104-855iw9wi 52 12 example example NN cord-300104-855iw9wi 52 13 a a DT cord-300104-855iw9wi 52 14 150 150 CD cord-300104-855iw9wi 52 15 ml ml NNP cord-300104-855iw9wi 52 16 medical medical NNP cord-300104-855iw9wi 52 17 fl fl NNP cord-300104-855iw9wi 52 18 at at IN cord-300104-855iw9wi 52 19 ) ) -RRB- cord-300104-855iw9wi 52 20 and and CC cord-300104-855iw9wi 52 21 wash wash VB cord-300104-855iw9wi 52 22 with with IN cord-300104-855iw9wi 52 23 approximately approximately RB cord-300104-855iw9wi 52 24 80 80 CD cord-300104-855iw9wi 52 25 ml ml NNS cord-300104-855iw9wi 52 26 of of IN cord-300104-855iw9wi 52 27 PBSa PBSa NNS cord-300104-855iw9wi 52 28 until until IN cord-300104-855iw9wi 52 29 the the DT cord-300104-855iw9wi 52 30 supernatant supernatant JJ cord-300104-855iw9wi 52 31 runs run VBZ cord-300104-855iw9wi 52 32 clear clear JJ cord-300104-855iw9wi 52 33 , , , cord-300104-855iw9wi 52 34 allowing allow VBG cord-300104-855iw9wi 52 35 the the DT cord-300104-855iw9wi 52 36 tissue tissue NN cord-300104-855iw9wi 52 37 fragments fragment NNS cord-300104-855iw9wi 52 38 to to TO cord-300104-855iw9wi 52 39 settle settle VB cord-300104-855iw9wi 52 40 for for IN cord-300104-855iw9wi 52 41 1 1 CD cord-300104-855iw9wi 52 42 min min NN cord-300104-855iw9wi 52 43 in in RP cord-300104-855iw9wi 52 44 between between IN cord-300104-855iw9wi 52 45 the the DT cord-300104-855iw9wi 52 46 washes wash NNS cord-300104-855iw9wi 52 47 and and CC cord-300104-855iw9wi 52 48 discarding discard VBG cord-300104-855iw9wi 52 49 the the DT cord-300104-855iw9wi 52 50 PBSa PBSa NNS cord-300104-855iw9wi 52 51 washes wash NNS cord-300104-855iw9wi 52 52 ( ( -LRB- cord-300104-855iw9wi 52 53 see see VB cord-300104-855iw9wi 52 54 Note Note NNP cord-300104-855iw9wi 52 55 6 6 CD cord-300104-855iw9wi 52 56 ) ) -RRB- cord-300104-855iw9wi 52 57 . . . cord-300104-855iw9wi 53 1 13 13 CD cord-300104-855iw9wi 53 2 . . . cord-300104-855iw9wi 54 1 Add add VB cord-300104-855iw9wi 54 2 50 50 CD cord-300104-855iw9wi 54 3 - - SYM cord-300104-855iw9wi 54 4 80 80 CD cord-300104-855iw9wi 54 5 ml ml NNP cord-300104-855iw9wi 54 6 Trypsin Trypsin NNP cord-300104-855iw9wi 54 7 / / SYM cord-300104-855iw9wi 54 8 EDTA EDTA NNP cord-300104-855iw9wi 54 9 to to IN cord-300104-855iw9wi 54 10 drained drained JJ cord-300104-855iw9wi 54 11 tissue tissue NN cord-300104-855iw9wi 54 12 and and CC cord-300104-855iw9wi 54 13 shake shake VB cord-300104-855iw9wi 54 14 moderately moderately RB cord-300104-855iw9wi 54 15 hard hard RB cord-300104-855iw9wi 54 16 for for IN cord-300104-855iw9wi 54 17 2 2 CD cord-300104-855iw9wi 54 18 min min NN cord-300104-855iw9wi 54 19 . . . cord-300104-855iw9wi 55 1 Allow allow VB cord-300104-855iw9wi 55 2 the the DT cord-300104-855iw9wi 55 3 tissue tissue NN cord-300104-855iw9wi 55 4 to to TO cord-300104-855iw9wi 55 5 settle settle VB cord-300104-855iw9wi 55 6 and and CC cord-300104-855iw9wi 55 7 discard discard VB cord-300104-855iw9wi 55 8 the the DT cord-300104-855iw9wi 55 9 supernatant supernatant NN cord-300104-855iw9wi 55 10 ( ( -LRB- cord-300104-855iw9wi 55 11 see see VB cord-300104-855iw9wi 55 12 Note Note NNP cord-300104-855iw9wi 55 13 6 6 CD cord-300104-855iw9wi 55 14 ) ) -RRB- cord-300104-855iw9wi 55 15 . . . cord-300104-855iw9wi 56 1 14 14 CD cord-300104-855iw9wi 56 2 . . . cord-300104-855iw9wi 57 1 Add add VB cord-300104-855iw9wi 57 2 another another DT cord-300104-855iw9wi 57 3 volume volume NN cord-300104-855iw9wi 57 4 of of IN cord-300104-855iw9wi 57 5 Trypsin Trypsin NNP cord-300104-855iw9wi 57 6 / / SYM cord-300104-855iw9wi 57 7 EDTA EDTA NNP cord-300104-855iw9wi 57 8 and and CC cord-300104-855iw9wi 57 9 shake shake VB cord-300104-855iw9wi 57 10 for for IN cord-300104-855iw9wi 57 11 4 4 CD cord-300104-855iw9wi 57 12 min min NN cord-300104-855iw9wi 57 13 . . . cord-300104-855iw9wi 58 1 Allow allow VB cord-300104-855iw9wi 58 2 the the DT cord-300104-855iw9wi 58 3 tissue tissue NN cord-300104-855iw9wi 58 4 to to TO cord-300104-855iw9wi 58 5 settle settle VB cord-300104-855iw9wi 58 6 and and CC cord-300104-855iw9wi 58 7 this this DT cord-300104-855iw9wi 58 8 time time NN cord-300104-855iw9wi 58 9 pour pour VBP cord-300104-855iw9wi 58 10 the the DT cord-300104-855iw9wi 58 11 supernatant supernatant NN cord-300104-855iw9wi 58 12 into into IN cord-300104-855iw9wi 58 13 the the DT cord-300104-855iw9wi 58 14 conical conical JJ cord-300104-855iw9wi 58 15 fl fl NNP cord-300104-855iw9wi 58 16 ask ask NN cord-300104-855iw9wi 58 17 containing contain VBG cord-300104-855iw9wi 58 18 NBBS NBBS NNP cord-300104-855iw9wi 58 19 . . . cord-300104-855iw9wi 59 1 Gently gently RB cord-300104-855iw9wi 59 2 swirl swirl VB cord-300104-855iw9wi 59 3 the the DT cord-300104-855iw9wi 59 4 fl fl NNP cord-300104-855iw9wi 59 5 ask ask NN cord-300104-855iw9wi 59 6 to to TO cord-300104-855iw9wi 59 7 distribute distribute VB cord-300104-855iw9wi 59 8 the the DT cord-300104-855iw9wi 59 9 isolated isolate VBN cord-300104-855iw9wi 59 10 cells cell NNS cord-300104-855iw9wi 59 11 in in IN cord-300104-855iw9wi 59 12 the the DT cord-300104-855iw9wi 59 13 NBBS NBBS NNP cord-300104-855iw9wi 59 14 . . . cord-300104-855iw9wi 60 1 15 15 CD cord-300104-855iw9wi 60 2 . . . cord-300104-855iw9wi 61 1 Repeat repeat VB cord-300104-855iw9wi 61 2 step step NN cord-300104-855iw9wi 61 3 14 14 CD cord-300104-855iw9wi 61 4 until until IN cord-300104-855iw9wi 61 5 no no DT cord-300104-855iw9wi 61 6 more more JJR cord-300104-855iw9wi 61 7 tissue tissue NN cord-300104-855iw9wi 61 8 remains remain VBZ cord-300104-855iw9wi 61 9 ( ( -LRB- cord-300104-855iw9wi 61 10 see see VB cord-300104-855iw9wi 61 11 Note Note NNP cord-300104-855iw9wi 61 12 7 7 CD cord-300104-855iw9wi 61 13 ) ) -RRB- cord-300104-855iw9wi 61 14 . . . cord-300104-855iw9wi 62 1 Step step NN cord-300104-855iw9wi 62 2 14 14 CD cord-300104-855iw9wi 62 3 through through IN cord-300104-855iw9wi 62 4 the the DT cord-300104-855iw9wi 62 5 metal metal NN cord-300104-855iw9wi 62 6 gauze gauze NN cord-300104-855iw9wi 62 7 fi fi NNP cord-300104-855iw9wi 62 8 lter lter NN cord-300104-855iw9wi 62 9 supported support VBN cord-300104-855iw9wi 62 10 in in IN cord-300104-855iw9wi 62 11 a a DT cord-300104-855iw9wi 62 12 funnel funnel NN cord-300104-855iw9wi 62 13 placed place VBN cord-300104-855iw9wi 62 14 in in IN cord-300104-855iw9wi 62 15 a a DT cord-300104-855iw9wi 62 16 fresh fresh JJ cord-300104-855iw9wi 62 17 conical conical JJ cord-300104-855iw9wi 62 18 fl fl NNP cord-300104-855iw9wi 62 19 ask ask NN cord-300104-855iw9wi 62 20 ( ( -LRB- cord-300104-855iw9wi 62 21 see see VB cord-300104-855iw9wi 62 22 Note Note NNP cord-300104-855iw9wi 62 23 8 8 CD cord-300104-855iw9wi 62 24 ) ) -RRB- cord-300104-855iw9wi 62 25 . . . cord-300104-855iw9wi 63 1 Decant decant VB cord-300104-855iw9wi 63 2 fi fi FW cord-300104-855iw9wi 63 3 ltered ltered JJ cord-300104-855iw9wi 63 4 cells cell NNS cord-300104-855iw9wi 63 5 into into IN cord-300104-855iw9wi 63 6 centrifuge centrifuge NN cord-300104-855iw9wi 63 7 tube(s tube(s NNP cord-300104-855iw9wi 63 8 ) ) -RRB- cord-300104-855iw9wi 63 9 and and CC cord-300104-855iw9wi 63 10 centrifuge centrifuge VB cord-300104-855iw9wi 63 11 at at IN cord-300104-855iw9wi 63 12 approximately approximately RB cord-300104-855iw9wi 63 13 300 300 CD cord-300104-855iw9wi 63 14 × × NN cord-300104-855iw9wi 63 15 g g NN cord-300104-855iw9wi 63 16 for for IN cord-300104-855iw9wi 63 17 10 10 CD cord-300104-855iw9wi 63 18 min min NN cord-300104-855iw9wi 63 19 to to TO cord-300104-855iw9wi 63 20 pellet pellet VB cord-300104-855iw9wi 63 21 the the DT cord-300104-855iw9wi 63 22 cells cell NNS cord-300104-855iw9wi 63 23 . . . cord-300104-855iw9wi 64 1 1 1 LS cord-300104-855iw9wi 64 2 . . . cord-300104-855iw9wi 65 1 Warm warm JJ cord-300104-855iw9wi 65 2 growth growth NN cord-300104-855iw9wi 65 3 medium medium NN cord-300104-855iw9wi 65 4 to to IN cord-300104-855iw9wi 65 5 37 37 CD cord-300104-855iw9wi 65 6 ° ° NN cord-300104-855iw9wi 65 7 C c NN cord-300104-855iw9wi 65 8 . . . cord-300104-855iw9wi 65 9 2 2 CD cord-300104-855iw9wi 65 10 . . . cord-300104-855iw9wi 66 1 Working work VBG cord-300104-855iw9wi 66 2 in in IN cord-300104-855iw9wi 66 3 a a DT cord-300104-855iw9wi 66 4 Microbiological Microbiological NNP cord-300104-855iw9wi 66 5 Safety Safety NNP cord-300104-855iw9wi 66 6 Cabinet Cabinet NNP cord-300104-855iw9wi 66 7 ( ( -LRB- cord-300104-855iw9wi 66 8 Class Class NNP cord-300104-855iw9wi 66 9 2 2 CD cord-300104-855iw9wi 66 10 ) ) -RRB- cord-300104-855iw9wi 66 11 , , , cord-300104-855iw9wi 66 12 carefully carefully RB cord-300104-855iw9wi 66 13 discard discard VB cord-300104-855iw9wi 66 14 the the DT cord-300104-855iw9wi 66 15 supernatant supernatant NN cord-300104-855iw9wi 66 16 from from IN cord-300104-855iw9wi 66 17 the the DT cord-300104-855iw9wi 66 18 centrifuge centrifuge NN cord-300104-855iw9wi 66 19 tubes tube NNS cord-300104-855iw9wi 66 20 and and CC cord-300104-855iw9wi 66 21 resuspend resuspend VB cord-300104-855iw9wi 66 22 the the DT cord-300104-855iw9wi 66 23 pelleted pelleted JJ cord-300104-855iw9wi 66 24 cells cell NNS cord-300104-855iw9wi 66 25 in in IN cord-300104-855iw9wi 66 26 growth growth NN cord-300104-855iw9wi 66 27 medium medium NN cord-300104-855iw9wi 66 28 , , , cord-300104-855iw9wi 66 29 triturating triturate VBG cord-300104-855iw9wi 66 30 at at IN cord-300104-855iw9wi 66 31 least least JJS cord-300104-855iw9wi 66 32 fi fi NNP cord-300104-855iw9wi 66 33 ve ve NNP cord-300104-855iw9wi 66 34 times time NNS cord-300104-855iw9wi 66 35 . . . cord-300104-855iw9wi 67 1 3 3 LS cord-300104-855iw9wi 67 2 . . . cord-300104-855iw9wi 68 1 Using use VBG cord-300104-855iw9wi 68 2 a a DT cord-300104-855iw9wi 68 3 50 50 CD cord-300104-855iw9wi 68 4 ml ml NN cord-300104-855iw9wi 68 5 syringe syringe NN cord-300104-855iw9wi 68 6 pass pass VB cord-300104-855iw9wi 68 7 the the DT cord-300104-855iw9wi 68 8 cell cell NN cord-300104-855iw9wi 68 9 suspension suspension NN cord-300104-855iw9wi 68 10 through through IN cord-300104-855iw9wi 68 11 Swinnex Swinnex NNP cord-300104-855iw9wi 68 12 fi fi NNP cord-300104-855iw9wi 68 13 lter lter NN cord-300104-855iw9wi 68 14 1 1 CD cord-300104-855iw9wi 68 15 then then RB cord-300104-855iw9wi 68 16 through through IN cord-300104-855iw9wi 68 17 Swinnex Swinnex NNP cord-300104-855iw9wi 68 18 fi fi NNP cord-300104-855iw9wi 68 19 lter lter NN cord-300104-855iw9wi 68 20 2 2 CD cord-300104-855iw9wi 68 21 collecting collect VBG cord-300104-855iw9wi 68 22 the the DT cord-300104-855iw9wi 68 23 fi fi NNP cord-300104-855iw9wi 68 24 ltrates ltrate NNS cord-300104-855iw9wi 68 25 from from IN cord-300104-855iw9wi 68 26 fi fi NNP cord-300104-855iw9wi 68 27 lter lter NNP cord-300104-855iw9wi 68 28 1 1 CD cord-300104-855iw9wi 68 29 and and CC cord-300104-855iw9wi 68 30 2 2 CD cord-300104-855iw9wi 68 31 in in IN cord-300104-855iw9wi 68 32 a a DT cord-300104-855iw9wi 68 33 fresh fresh JJ cord-300104-855iw9wi 68 34 fl fl NNP cord-300104-855iw9wi 69 1 ask ask VB cord-300104-855iw9wi 69 2 each each DT cord-300104-855iw9wi 69 3 time time NN cord-300104-855iw9wi 69 4 ( ( -LRB- cord-300104-855iw9wi 69 5 see see VB cord-300104-855iw9wi 69 6 Note Note NNP cord-300104-855iw9wi 69 7 8 8 CD cord-300104-855iw9wi 69 8 ) ) -RRB- cord-300104-855iw9wi 69 9 . . . cord-300104-855iw9wi 70 1 4 4 LS cord-300104-855iw9wi 70 2 . . . cord-300104-855iw9wi 71 1 Measure measure NN cord-300104-855iw9wi 71 2 and and CC cord-300104-855iw9wi 71 3 record record VB cord-300104-855iw9wi 71 4 the the DT cord-300104-855iw9wi 71 5 volume volume NN cord-300104-855iw9wi 71 6 . . . cord-300104-855iw9wi 72 1 Take take VB cord-300104-855iw9wi 72 2 0.1 0.1 CD cord-300104-855iw9wi 72 3 ml ml NNS cord-300104-855iw9wi 72 4 of of IN cord-300104-855iw9wi 72 5 cell cell NN cord-300104-855iw9wi 72 6 suspension suspension NN cord-300104-855iw9wi 72 7 add add VB cord-300104-855iw9wi 72 8 to to IN cord-300104-855iw9wi 72 9 0.9 0.9 CD cord-300104-855iw9wi 72 10 ml ml NNS cord-300104-855iw9wi 72 11 of of IN cord-300104-855iw9wi 72 12 trypan trypan NNP cord-300104-855iw9wi 72 13 blue blue NNP cord-300104-855iw9wi 72 14 and and CC cord-300104-855iw9wi 72 15 count count VB cord-300104-855iw9wi 72 16 the the DT cord-300104-855iw9wi 72 17 viable viable JJ cord-300104-855iw9wi 72 18 cells cell NNS cord-300104-855iw9wi 72 19 ( ( -LRB- cord-300104-855iw9wi 72 20 see see VB cord-300104-855iw9wi 72 21 Note Note NNP cord-300104-855iw9wi 72 22 9 9 CD cord-300104-855iw9wi 72 23 ) ) -RRB- cord-300104-855iw9wi 72 24 . . . cord-300104-855iw9wi 73 1 5 5 LS cord-300104-855iw9wi 73 2 . . . cord-300104-855iw9wi 74 1 Dilute dilute JJ cord-300104-855iw9wi 74 2 cell cell NN cord-300104-855iw9wi 74 3 suspension suspension NN cord-300104-855iw9wi 74 4 in in IN cord-300104-855iw9wi 74 5 growth growth NN cord-300104-855iw9wi 74 6 medium medium NN cord-300104-855iw9wi 74 7 to to IN cord-300104-855iw9wi 74 8 the the DT cord-300104-855iw9wi 74 9 cell cell NN cord-300104-855iw9wi 74 10 concentration concentration NN cord-300104-855iw9wi 74 11 required require VBN cord-300104-855iw9wi 74 12 , , , cord-300104-855iw9wi 74 13 seed seed NN cord-300104-855iw9wi 74 14 culture culture NN cord-300104-855iw9wi 74 15 fl fl NNP cord-300104-855iw9wi 74 16 asks ask VBZ cord-300104-855iw9wi 74 17 and and CC cord-300104-855iw9wi 74 18 place place NN cord-300104-855iw9wi 74 19 in in IN cord-300104-855iw9wi 74 20 incubator incubator NN cord-300104-855iw9wi 74 21 until until IN cord-300104-855iw9wi 74 22 intact intact JJ cord-300104-855iw9wi 74 23 monolayer monolayer NN cord-300104-855iw9wi 74 24 forms form NNS cord-300104-855iw9wi 74 25 ( ( -LRB- cord-300104-855iw9wi 74 26 see see VB cord-300104-855iw9wi 74 27 Note Note NNP cord-300104-855iw9wi 74 28 10 10 CD cord-300104-855iw9wi 74 29 ) ) -RRB- cord-300104-855iw9wi 74 30 . . . cord-300104-855iw9wi 75 1 1 1 LS cord-300104-855iw9wi 75 2 . . . cord-300104-855iw9wi 76 1 This this DT cord-300104-855iw9wi 76 2 should should MD cord-300104-855iw9wi 76 3 be be VB cord-300104-855iw9wi 76 4 done do VBN cord-300104-855iw9wi 76 5 immediately immediately RB cord-300104-855iw9wi 76 6 prior prior RB cord-300104-855iw9wi 76 7 to to IN cord-300104-855iw9wi 76 8 the the DT cord-300104-855iw9wi 76 9 removal removal NN cord-300104-855iw9wi 76 10 of of IN cord-300104-855iw9wi 76 11 the the DT cord-300104-855iw9wi 76 12 kidneys kidney NNS cord-300104-855iw9wi 76 13 to to TO cord-300104-855iw9wi 76 14 minimize minimize VB cord-300104-855iw9wi 76 15 the the DT cord-300104-855iw9wi 76 16 buildup buildup NN cord-300104-855iw9wi 76 17 of of IN cord-300104-855iw9wi 76 18 blood blood NN cord-300104-855iw9wi 76 19 clots clot NNS cord-300104-855iw9wi 76 20 . . . cord-300104-855iw9wi 77 1 2 2 LS cord-300104-855iw9wi 77 2 . . . cord-300104-855iw9wi 78 1 Wire wire NN cord-300104-855iw9wi 78 2 mesh mesh NN cord-300104-855iw9wi 78 3 is be VBZ cord-300104-855iw9wi 78 4 obtainable obtainable JJ cord-300104-855iw9wi 78 5 from from IN cord-300104-855iw9wi 78 6 Locker Locker NNP cord-300104-855iw9wi 78 7 Wire Wire NNP cord-300104-855iw9wi 78 8 Weavers Weavers NNPS cord-300104-855iw9wi 78 9 , , , cord-300104-855iw9wi 78 10 www www NN cord-300104-855iw9wi 78 11 . . . cord-300104-855iw9wi 79 1 wiremesh.co.uk wiremesh.co.uk FW cord-300104-855iw9wi 79 2 . . . cord-300104-855iw9wi 80 1 3 3 LS cord-300104-855iw9wi 80 2 . . . cord-300104-855iw9wi 81 1 As as IN cord-300104-855iw9wi 81 2 one one CD cord-300104-855iw9wi 81 3 batch batch NN cord-300104-855iw9wi 81 4 of of IN cord-300104-855iw9wi 81 5 serum serum NN cord-300104-855iw9wi 81 6 may may MD cord-300104-855iw9wi 81 7 not not RB cord-300104-855iw9wi 81 8 support support VB cord-300104-855iw9wi 81 9 the the DT cord-300104-855iw9wi 81 10 growth growth NN cord-300104-855iw9wi 81 11 of of IN cord-300104-855iw9wi 81 12 CK CK NNP cord-300104-855iw9wi 81 13 cells cell NNS cord-300104-855iw9wi 81 14 as as RB cord-300104-855iw9wi 81 15 well well RB cord-300104-855iw9wi 81 16 as as IN cord-300104-855iw9wi 81 17 another another DT cord-300104-855iw9wi 81 18 , , , cord-300104-855iw9wi 81 19 we -PRON- PRP cord-300104-855iw9wi 81 20 recommend recommend VBP cord-300104-855iw9wi 81 21 batch batch JJ cord-300104-855iw9wi 81 22 testing testing NN cord-300104-855iw9wi 81 23 of of IN cord-300104-855iw9wi 81 24 serum serum NN cord-300104-855iw9wi 81 25 prior prior RB cord-300104-855iw9wi 81 26 to to TO cord-300104-855iw9wi 81 27 purchase purchase VB cord-300104-855iw9wi 81 28 and and CC cord-300104-855iw9wi 81 29 use use VB cord-300104-855iw9wi 81 30 . . . cord-300104-855iw9wi 82 1 5 5 LS cord-300104-855iw9wi 82 2 . . . cord-300104-855iw9wi 83 1 The the DT cord-300104-855iw9wi 83 2 volume volume NN cord-300104-855iw9wi 83 3 of of IN cord-300104-855iw9wi 83 4 NBBS NBBS NNP cord-300104-855iw9wi 83 5 , , , cord-300104-855iw9wi 83 6 which which WDT cord-300104-855iw9wi 83 7 is be VBZ cord-300104-855iw9wi 83 8 used use VBN cord-300104-855iw9wi 83 9 to to TO cord-300104-855iw9wi 83 10 inactivate inactivate VB cord-300104-855iw9wi 83 11 the the DT cord-300104-855iw9wi 83 12 Trypsin/ Trypsin/ NNP cord-300104-855iw9wi 83 13 EDTA EDTA NNP cord-300104-855iw9wi 83 14 , , , cord-300104-855iw9wi 83 15 will will MD cord-300104-855iw9wi 83 16 depend depend VB cord-300104-855iw9wi 83 17 on on IN cord-300104-855iw9wi 83 18 the the DT cord-300104-855iw9wi 83 19 number number NN cord-300104-855iw9wi 83 20 of of IN cord-300104-855iw9wi 83 21 birds bird NNS cord-300104-855iw9wi 83 22 . . . cord-300104-855iw9wi 84 1 Use use VB cord-300104-855iw9wi 84 2 at at RB cord-300104-855iw9wi 84 3 least least JJS cord-300104-855iw9wi 84 4 50 50 CD cord-300104-855iw9wi 84 5 ml ml NNS cord-300104-855iw9wi 84 6 for for IN cord-300104-855iw9wi 84 7 up up IN cord-300104-855iw9wi 84 8 to to TO cord-300104-855iw9wi 84 9 6 6 CD cord-300104-855iw9wi 84 10 birds bird NNS cord-300104-855iw9wi 84 11 and and CC cord-300104-855iw9wi 84 12 increase increase VB cord-300104-855iw9wi 84 13 the the DT cord-300104-855iw9wi 84 14 volume volume NN cord-300104-855iw9wi 84 15 by by IN cord-300104-855iw9wi 84 16 10 10 CD cord-300104-855iw9wi 84 17 ml ml NNS cord-300104-855iw9wi 84 18 for for IN cord-300104-855iw9wi 84 19 each each DT cord-300104-855iw9wi 84 20 extra extra JJ cord-300104-855iw9wi 84 21 bird bird NN cord-300104-855iw9wi 84 22 . . . cord-300104-855iw9wi 85 1 The the DT cord-300104-855iw9wi 85 2 fl fl NNP cord-300104-855iw9wi 85 3 ask ask NN cord-300104-855iw9wi 85 4 volume volume NN cord-300104-855iw9wi 85 5 should should MD cord-300104-855iw9wi 85 6 be be VB cord-300104-855iw9wi 85 7 at at RB cord-300104-855iw9wi 85 8 least least JJS cord-300104-855iw9wi 85 9 500 500 CD cord-300104-855iw9wi 85 10 ml ml NN cord-300104-855iw9wi 85 11 . . NN cord-300104-855iw9wi 85 12 6 6 CD cord-300104-855iw9wi 85 13 . . . cord-300104-855iw9wi 86 1 Whilst whilst IN cord-300104-855iw9wi 86 2 some some DT cord-300104-855iw9wi 86 3 kidney kidney NN cord-300104-855iw9wi 86 4 cells cell NNS cord-300104-855iw9wi 86 5 may may MD cord-300104-855iw9wi 86 6 be be VB cord-300104-855iw9wi 86 7 lost lose VBN cord-300104-855iw9wi 86 8 in in IN cord-300104-855iw9wi 86 9 this this DT cord-300104-855iw9wi 86 10 process process NN cord-300104-855iw9wi 86 11 , , , cord-300104-855iw9wi 86 12 it -PRON- PRP cord-300104-855iw9wi 86 13 is be VBZ cord-300104-855iw9wi 86 14 an an DT cord-300104-855iw9wi 86 15 effective effective JJ cord-300104-855iw9wi 86 16 way way NN cord-300104-855iw9wi 86 17 of of IN cord-300104-855iw9wi 86 18 removing remove VBG cord-300104-855iw9wi 86 19 many many JJ cord-300104-855iw9wi 86 20 of of IN cord-300104-855iw9wi 86 21 the the DT cord-300104-855iw9wi 86 22 red red JJ cord-300104-855iw9wi 86 23 blood blood NN cord-300104-855iw9wi 86 24 cells cell NNS cord-300104-855iw9wi 86 25 that that WDT cord-300104-855iw9wi 86 26 are be VBP cord-300104-855iw9wi 86 27 still still RB cord-300104-855iw9wi 86 28 present present JJ cord-300104-855iw9wi 86 29 at at IN cord-300104-855iw9wi 86 30 this this DT cord-300104-855iw9wi 86 31 stage stage NN cord-300104-855iw9wi 86 32 of of IN cord-300104-855iw9wi 86 33 the the DT cord-300104-855iw9wi 86 34 preparation preparation NN cord-300104-855iw9wi 86 35 . . . cord-300104-855iw9wi 87 1 7 7 CD cord-300104-855iw9wi 87 2 . . . cord-300104-855iw9wi 88 1 This this DT cord-300104-855iw9wi 88 2 may may MD cord-300104-855iw9wi 88 3 require require VB cord-300104-855iw9wi 88 4 6 6 CD cord-300104-855iw9wi 88 5 - - SYM cord-300104-855iw9wi 88 6 8 8 CD cord-300104-855iw9wi 88 7 repeats repeat NNS cord-300104-855iw9wi 88 8 . . . cord-300104-855iw9wi 89 1 8 8 CD cord-300104-855iw9wi 89 2 . . . cord-300104-855iw9wi 90 1 This this DT cord-300104-855iw9wi 90 2 process process NN cord-300104-855iw9wi 90 3 helps help VBZ cord-300104-855iw9wi 90 4 to to TO cord-300104-855iw9wi 90 5 remove remove VB cord-300104-855iw9wi 90 6 some some DT cord-300104-855iw9wi 90 7 of of IN cord-300104-855iw9wi 90 8 the the DT cord-300104-855iw9wi 90 9 larger large JJR cord-300104-855iw9wi 90 10 aggregates aggregate NNS cord-300104-855iw9wi 90 11 of of IN cord-300104-855iw9wi 90 12 cells cell NNS cord-300104-855iw9wi 90 13 that that WDT cord-300104-855iw9wi 90 14 remain remain VBP cord-300104-855iw9wi 90 15 in in IN cord-300104-855iw9wi 90 16 the the DT cord-300104-855iw9wi 90 17 preparation preparation NN cord-300104-855iw9wi 90 18 at at IN cord-300104-855iw9wi 90 19 this this DT cord-300104-855iw9wi 90 20 stage stage NN cord-300104-855iw9wi 90 21 . . . cord-300104-855iw9wi 91 1 Removing remove VBG cord-300104-855iw9wi 91 2 the the DT cord-300104-855iw9wi 91 3 aggregates aggregate NNS cord-300104-855iw9wi 91 4 makes make VBZ cord-300104-855iw9wi 91 5 the the DT cord-300104-855iw9wi 91 6 cell cell NN cord-300104-855iw9wi 91 7 counting counting NN cord-300104-855iw9wi 91 8 process process NN cord-300104-855iw9wi 91 9 more more RBR cord-300104-855iw9wi 91 10 accurate accurate JJ cord-300104-855iw9wi 91 11 and and CC cord-300104-855iw9wi 91 12 the the DT cord-300104-855iw9wi 91 13 resultant resultant JJ cord-300104-855iw9wi 91 14 monolayers monolayer NNS cord-300104-855iw9wi 91 15 more more RBR cord-300104-855iw9wi 91 16 evenly evenly RB cord-300104-855iw9wi 91 17 dispersed disperse VBN cord-300104-855iw9wi 91 18 . . . cord-300104-855iw9wi 92 1 9 9 LS cord-300104-855iw9wi 92 2 . . . cord-300104-855iw9wi 93 1 There there EX cord-300104-855iw9wi 93 2 will will MD cord-300104-855iw9wi 93 3 still still RB cord-300104-855iw9wi 93 4 be be VB cord-300104-855iw9wi 93 5 a a DT cord-300104-855iw9wi 93 6 number number NN cord-300104-855iw9wi 93 7 of of IN cord-300104-855iw9wi 93 8 red red JJ cord-300104-855iw9wi 93 9 blood blood NN cord-300104-855iw9wi 93 10 cells cell NNS cord-300104-855iw9wi 93 11 at at IN cord-300104-855iw9wi 93 12 this this DT cord-300104-855iw9wi 93 13 stage stage NN cord-300104-855iw9wi 93 14 . . . cord-300104-855iw9wi 94 1 These these DT cord-300104-855iw9wi 94 2 should should MD cord-300104-855iw9wi 94 3 be be VB cord-300104-855iw9wi 94 4 excluded exclude VBN cord-300104-855iw9wi 94 5 from from IN cord-300104-855iw9wi 94 6 the the DT cord-300104-855iw9wi 94 7 cell cell NN cord-300104-855iw9wi 94 8 count count NN cord-300104-855iw9wi 94 9 . . . cord-300104-855iw9wi 95 1 The the DT cord-300104-855iw9wi 95 2 red red JJ cord-300104-855iw9wi 95 3 blood blood NN cord-300104-855iw9wi 95 4 cells cell NNS cord-300104-855iw9wi 95 5 can can MD cord-300104-855iw9wi 95 6 be be VB cord-300104-855iw9wi 95 7 distinguished distinguish VBN cord-300104-855iw9wi 95 8 from from IN cord-300104-855iw9wi 95 9 other other JJ cord-300104-855iw9wi 95 10 kidney kidney NN cord-300104-855iw9wi 95 11 cells cell NNS cord-300104-855iw9wi 95 12 by by IN cord-300104-855iw9wi 95 13 their -PRON- PRP$ cord-300104-855iw9wi 95 14 size size NN cord-300104-855iw9wi 95 15 and and CC cord-300104-855iw9wi 95 16 shape shape NN cord-300104-855iw9wi 95 17 . . . cord-300104-855iw9wi 96 1 10 10 CD cord-300104-855iw9wi 96 2 . . . cord-300104-855iw9wi 97 1 For for IN cord-300104-855iw9wi 97 2 plaque plaque NN cord-300104-855iw9wi 97 3 assays assay NNS cord-300104-855iw9wi 97 4 and and CC cord-300104-855iw9wi 97 5 viral viral JJ cord-300104-855iw9wi 97 6 growth growth NN cord-300104-855iw9wi 97 7 seed seed NN cord-300104-855iw9wi 97 8 fl fl NNP cord-300104-855iw9wi 97 9 asks ask VBZ cord-300104-855iw9wi 97 10 at at IN cord-300104-855iw9wi 97 11 approximately approximately RB cord-300104-855iw9wi 97 12 0.3 0.3 CD cord-300104-855iw9wi 97 13 × × NN cord-300104-855iw9wi 97 14 10 10 CD cord-300104-855iw9wi 97 15 6 6 CD cord-300104-855iw9wi 97 16 /cm /cm SYM cord-300104-855iw9wi 97 17 2 2 CD cord-300104-855iw9wi 97 18 and and CC cord-300104-855iw9wi 97 19 incubate incubate VB cord-300104-855iw9wi 97 20 for for IN cord-300104-855iw9wi 97 21 72 72 CD cord-300104-855iw9wi 97 22 h h NN cord-300104-855iw9wi 97 23 prior prior RB cord-300104-855iw9wi 97 24 to to IN cord-300104-855iw9wi 97 25 virus virus NN cord-300104-855iw9wi 97 26 introduction introduction NN cord-300104-855iw9wi 97 27 . . . cord-300104-855iw9wi 97 28 _SP cord-300104-855iw9wi 98 1 Plaque plaque NN cord-300104-855iw9wi 98 2 formation formation NN cord-300104-855iw9wi 98 3 and and CC cord-300104-855iw9wi 98 4 isolation isolation NN cord-300104-855iw9wi 98 5 of of IN cord-300104-855iw9wi 98 6 pure pure JJ cord-300104-855iw9wi 98 7 lines line NNS cord-300104-855iw9wi 98 8 with with IN cord-300104-855iw9wi 98 9 poliomyelitis poliomyelitis NN cord-300104-855iw9wi 98 10 viruses virus NNS cord-300104-855iw9wi 98 11 Monolayer Monolayer NNP cord-300104-855iw9wi 98 12 tissue tissue NN cord-300104-855iw9wi 98 13 cultures culture NNS cord-300104-855iw9wi 98 14 . . . cord-300104-855iw9wi 99 1 1 1 LS cord-300104-855iw9wi 99 2 . . . cord-300104-855iw9wi 100 1 Preparation preparation NN cord-300104-855iw9wi 100 2 and and CC cord-300104-855iw9wi 100 3 standardization standardization NN cord-300104-855iw9wi 100 4 of of IN cord-300104-855iw9wi 100 5 suspensions suspension NNS cord-300104-855iw9wi 100 6 of of IN cord-300104-855iw9wi 100 7 trypsin trypsin NN cord-300104-855iw9wi 100 8 - - HYPH cord-300104-855iw9wi 100 9 dispersed disperse VBN cord-300104-855iw9wi 100 10 monkey monkey NN cord-300104-855iw9wi 100 11 kidney kidney NN cord-300104-855iw9wi 100 12 cells cell NNS cord-300104-855iw9wi 101 1 The the DT cord-300104-855iw9wi 101 2 propagation propagation NN cord-300104-855iw9wi 101 3 of of IN cord-300104-855iw9wi 101 4 multiple multiple JJ cord-300104-855iw9wi 101 5 viruses virus NNS cord-300104-855iw9wi 101 6 in in IN cord-300104-855iw9wi 101 7 chick chick NN cord-300104-855iw9wi 101 8 kidney kidney NN cord-300104-855iw9wi 101 9 cultures culture NNS cord-300104-855iw9wi 102 1 The the DT cord-300104-855iw9wi 102 2 use use NN cord-300104-855iw9wi 102 3 of of IN cord-300104-855iw9wi 102 4 chicken chicken NN cord-300104-855iw9wi 102 5 kidney kidney NN cord-300104-855iw9wi 102 6 tissue tissue NN cord-300104-855iw9wi 102 7 culture culture NN cord-300104-855iw9wi 102 8 in in IN cord-300104-855iw9wi 102 9 the the DT cord-300104-855iw9wi 102 10 study study NN cord-300104-855iw9wi 102 11 of of IN cord-300104-855iw9wi 102 12 the the DT cord-300104-855iw9wi 102 13 avian avian JJ cord-300104-855iw9wi 102 14 viruses virus NNS cord-300104-855iw9wi 102 15 of of IN cord-300104-855iw9wi 102 16 Newcastle Newcastle NNP cord-300104-855iw9wi 102 17 Disease Disease NNP cord-300104-855iw9wi 102 18 , , , cord-300104-855iw9wi 102 19 Infectious Infectious NNP cord-300104-855iw9wi 102 20 Laryngo Laryngo NNP cord-300104-855iw9wi 102 21 Tracheitis Tracheitis NNP cord-300104-855iw9wi 102 22 and and CC cord-300104-855iw9wi 102 23 Infectious Infectious NNP cord-300104-855iw9wi 102 24 Bronchitis Bronchitis NNP